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<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC0</accession>
    <name>Q67JC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3248"/>
    <dbReference type="EnsemblBacteria" id="BAD42230">
        <property type="protein sequence ID" value="BAD42230"/>
        <property type="gene ID" value="STH3248"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3248"/>
    <dbReference type="eggNOG" id="COG1978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128607_0_0_9"/>
    <dbReference type="OMA" id="MYFISPT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007405">
        <property type="entry name" value="Phage_KVP40_Orf299"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39961">
        <property type="entry name" value="PTHR39961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04308">
        <property type="entry name" value="RNaseH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="17828" checksum="25CA57FF12FC0BC1" modified="2004-10-11" version="1">MEDGRFYSPTRGPLTLDQVVEDLLAFMADDPHGSYRLIVGTDSQTRTDLCFVTAIIVHRKGKGARFYYRRRRQSKMRSLRQRIFYEAALSLATAERLAQCLSNCGATGLNVEIHLDVGEHGETRELIRDVVGMIAGSGFHAEIKPNSYGASKVADRYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE2</accession>
    <name>Q67JE2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197339.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42208">
        <property type="protein sequence ID" value="BAD42208"/>
        <property type="gene ID" value="STH3226"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3226"/>
    <dbReference type="HOGENOM" id="CLU_2060232_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007060">
        <property type="entry name" value="FtsL/DivIC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04977">
        <property type="entry name" value="DivIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="119" mass="13482" checksum="722E0D30B5F07E71" modified="2004-10-11" version="1">MRTPYRRADTAATAPEPGRRARGDPLRRHWLTGALIAAVGAVLSFAALELYEARMQLEHALQAQAQVESELRALQEKNRRLSETLERVTSDESMELKAKQLGFTWPDEQVYQTIVPRGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK8</accession>
    <name>Y3160_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase STH3160</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH3160</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197273.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JK8"/>
    <dbReference type="SMR" id="Q67JK8"/>
    <dbReference type="STRING" id="292459.STH3160"/>
    <dbReference type="EnsemblBacteria" id="BAD42142">
        <property type="protein sequence ID" value="BAD42142"/>
        <property type="gene ID" value="STH3160"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3160"/>
    <dbReference type="eggNOG" id="COG2220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_4_1_9"/>
    <dbReference type="OMA" id="WHGHSTW"/>
    <dbReference type="OrthoDB" id="767622at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000367220" description="UPF0173 metal-dependent hydrolase STH3160">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <sequence length="224" mass="24069" checksum="463847F35B5A5639" modified="2004-10-11" version="1">MQIRYLGHSAFEITDGKWNLLIDPFITGNPACPVKAEELHPQYILVTHLHDDHVGDTVAIAKRTGATVITSFEGGQALAAQGVKVADMALGGKRRFDFGLVRVTLAFHGFGPTGGHACGFVIHIGGKRIYHAGDTALFSDMKLLNGVIEEPGIDVAMLPIGDNYTMGPEDAAVAVEWIRPKVVIPMHWGTFPVLVQDASGFAARVRETGASQPVVLRPGETYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL1</accession>
    <name>Q67JL1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 9">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">greA</name>
        <name evidence="12" type="synonym">greA2</name>
        <name evidence="12" type="ordered locus">STH3157</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197270.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3157"/>
    <dbReference type="EnsemblBacteria" id="BAD42139">
        <property type="protein sequence ID" value="BAD42139"/>
        <property type="gene ID" value="STH3157"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3157"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_2_1_9"/>
    <dbReference type="OMA" id="TWLTQEA"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="11">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="10">
        <location>
            <begin position="84"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17618" checksum="42B232373D425FDD" modified="2004-10-11" version="1">MAEKEVLLSLDGLKQLEQELLYLRTVKRMEVAERIKQAREYGDISENSEYEDAKNEQAFIEGRILVLEKMLRNARVIDEPVGQQEVVVLGSTVVLRDLEYGDEHTYTIVGTTEADPAHNRISNESPVGRAIMGQRVGTVVAVDAPDGVFKYEIVAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL4</accession>
    <name>Q67JL4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3154"/>
    <dbReference type="EnsemblBacteria" id="BAD42136">
        <property type="protein sequence ID" value="BAD42136"/>
        <property type="gene ID" value="STH3154"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3154"/>
    <dbReference type="HOGENOM" id="CLU_1354028_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269866" evidence="2">
        <location>
            <begin position="29"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="202" mass="21575" checksum="480018DF3207FCA0" modified="2004-10-11" version="1">MVRWARSPATALPVATLLLLLLPGKAAASHLSGGESDLMGIYGGVALVLGFAGLLGVLGLVPARWRWRGYRWGRWLLAAGLLVLIGGGFHAATVSAVEQRAGDEAFGRLATQQLFGTLAVFLAFWGGVYYLALRSGVLNMGESVKYAVLRNGDPPDRTAFRRERPGEHRLMWIPVAAMGILAAFFTAGVLIAILRLPPGARL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-21" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT3</accession>
    <name>RPOB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="1">RNAP subunit beta</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoB</name>
        <name type="ordered locus">STH3085</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197200.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JT3"/>
    <dbReference type="SMR" id="Q67JT3"/>
    <dbReference type="STRING" id="292459.STH3085"/>
    <dbReference type="EnsemblBacteria" id="BAD42067">
        <property type="protein sequence ID" value="BAD42067"/>
        <property type="gene ID" value="STH3085"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3085"/>
    <dbReference type="eggNOG" id="COG0085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_4_1_9"/>
    <dbReference type="OMA" id="FMTWEGY"/>
    <dbReference type="OrthoDB" id="9601at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000224113" description="DNA-directed RNA polymerase subunit beta">
        <location>
            <begin position="1"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1139"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1208"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1250" mass="139532" checksum="0AEA3C755AB24C48" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV6</accession>
    <name>RS14Z_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S14 type Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsZ</name>
        <name evidence="1" type="synonym">rpsN</name>
        <name type="ordered locus">STH3062</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S3 and S10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197177.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV6"/>
    <dbReference type="SMR" id="Q67JV6"/>
    <dbReference type="STRING" id="292459.STH3062"/>
    <dbReference type="EnsemblBacteria" id="BAD42044">
        <property type="protein sequence ID" value="BAD42044"/>
        <property type="gene ID" value="STH3062"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3062"/>
    <dbReference type="eggNOG" id="COG0199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_3_0_9"/>
    <dbReference type="OMA" id="RAYTRCN"/>
    <dbReference type="OrthoDB" id="2032609at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_B">
        <property type="entry name" value="Ribosomal_S14_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023053">
        <property type="entry name" value="Ribosomal_S14_Z"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="PTHR19836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000269151" description="30S ribosomal protein S14 type Z">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <sequence length="61" mass="7150" checksum="7C877CD99E3F89AD" modified="2004-10-11" version="1">MAKTSLKVKAARPQKFKVRAYNRCKLCGRPRAYMRKFGLCRHCFRKLAHEGQIPGIRKASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ0</accession>
    <name>Q67JZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine proteinase inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3027"/>
    <dbReference type="EnsemblBacteria" id="BAD42010">
        <property type="protein sequence ID" value="BAD42010"/>
        <property type="gene ID" value="STH3027"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3027"/>
    <dbReference type="eggNOG" id="COG4826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_0_3_9"/>
    <dbReference type="OMA" id="KDVWRGL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="PTHR11461"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="SSF56574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERPIN" evidence="2">
        <location>
            <begin position="14"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="371" mass="40357" checksum="55483023EF8EB026" modified="2004-10-11" version="1">MDPNLASAADRFGIRLLQEVHRERGAGNLFLSPASAQIILSLAANGARGETREEMLEAMGYGDMDLGAVNRAIADLRGILAHPGAGVELTAANAVWHQKGMTVAPAFREVAVQQYGAQVRETTFGEPASAEAINRWVSDQTRGRIPQLVEGTRPDDVMWLVNVLYFKGAWQKPFDPARTYDRPFHLPDGGTAEVPFMHQTAVFGYLAEDGLVGVRLPYGEGDLALYAFMPDEWEGFVEGLTPERYAGWVERMGRQEIRLAFPKIRLTDRAELADPLAAMGMERAFTPGRADLGGLFEGSRELFISRVIQKTFLEINEEGTEAAAATGVAVTVTSAPAGQPPEVVLDKPFLLAIRDDRTGVTLFLGAIADPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K00</accession>
    <name>Q67K00_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3017"/>
    <dbReference type="EnsemblBacteria" id="BAD42000">
        <property type="protein sequence ID" value="BAD42000"/>
        <property type="gene ID" value="STH3017"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3017"/>
    <dbReference type="eggNOG" id="COG2768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1642874_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="105"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="16601" checksum="6834A42D99F7AD21" modified="2004-10-11" version="1">MLQPHCNIGAHVRAILRSDYSRASTLALPAGDERSPREPGGTGGAGAVPLQNRPRPGPDPTAIAVISPHGLSVPGCVTLRAGDVLGVSTITGLTPFGRLFSPKRSQPPVNPTAPERSCGQCMACVAACPSGALTHPGDVWRLDLSICLGCRLCHLACPNPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K20</accession>
    <name>Q67K20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagellar hook-length control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fliK</name>
        <name evidence="3" type="ordered locus">STH2997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197112.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2997"/>
    <dbReference type="EnsemblBacteria" id="BAD41980">
        <property type="protein sequence ID" value="BAD41980"/>
        <property type="gene ID" value="STH2997"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2997"/>
    <dbReference type="eggNOG" id="COG3144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_619517_0_0_9"/>
    <dbReference type="OMA" id="PANHATH"/>
    <dbReference type="OrthoDB" id="1796543at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038610">
        <property type="entry name" value="FliK-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="2">
        <location>
            <begin position="321"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="442" mass="43001" checksum="B6CE6CA40380726C" modified="2004-10-11" version="1">MTAPIVSAGGAPQAPVATRGEGRRRSEASPADGTFAAVLAALAPMPSPPDAPGAGAAVPTGQAPPAGAGLPDPIQALLGPANPAGPQAMLPAAFPGEAAGAPDGQADAAAAPAGLTVEVIAAEQAAPGQAGQDPLANLLRPQGPEPPGPGTGDLPAGSPAQGGQAAPAEAGEAWMAARDRPQPAVLPDGAPAGQAAEADAAARPSAGPAPGGMPDGAARPSAGPAPGGMPDGAAVRGATESLASEGSAAGDGASAEEGSPQGTAVRQAPDAAPATGPDPGGGAEVAARPVSMRGLERLTAGRLQAEHLVNELTRALPELPDGQYRLTLRLHPEHLGEVRLELHLSGREVYAAMEVSSADARQALESRGEQLRQGLSDAGYQLAGFEVATGQGRQARQGGDEDPAGWPRASGRSVKATEAEAALAAAAGRSAGPRSGRLDTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K23</accession>
    <name>Q67K23_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Flagellar protein FliL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fliL</name>
        <name evidence="9" type="ordered locus">STH2994</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Controls the rotational direction of flagella during chemotaxis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the FliL family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197109.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2994"/>
    <dbReference type="EnsemblBacteria" id="BAD41977">
        <property type="protein sequence ID" value="BAD41977"/>
        <property type="gene ID" value="STH2994"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2994"/>
    <dbReference type="eggNOG" id="COG1580">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703346_0_0_9"/>
    <dbReference type="OrthoDB" id="1873899at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005503">
        <property type="entry name" value="FliL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35091">
        <property type="entry name" value="PTHR35091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03748">
        <property type="entry name" value="FliL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16033" checksum="5B851C1F8C794F4F" modified="2004-10-11" version="1">MNRGLITALVAAVVILAVGVAGLAAYVIFILPQSGGLVAAGGGGGTAVVAGEPVLEAAYYELDEFVTNLADKDRLRYIKFSVALGLRSDNSEQIARDAAPQVRDVVLSQVRELTAADLSGADGKERLAQAIKNGLDGLLPGHVTRVYVTDMVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K41</accession>
    <name>Q67K41_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliC</name>
        <name evidence="6" type="ordered locus">STH2974</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197089.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2974"/>
    <dbReference type="EnsemblBacteria" id="BAD41957">
        <property type="protein sequence ID" value="BAD41957"/>
        <property type="gene ID" value="STH2974"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2974"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011142_2_2_9"/>
    <dbReference type="OMA" id="FEHTINN"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042187">
        <property type="entry name" value="Flagellin_C_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00207">
        <property type="entry name" value="FLAGELLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="190"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="29768" checksum="7AD3154809AB75AB" modified="2004-10-11" version="1">MRINTNLAALNAWRALTINHGAMTKSLEKLSSGYRVNRAADDAAGLAVSEKMRAQIRGLNMAVRNAQDGVSLIQTAEGGAQKIQDMLQRMRELAVQAASDTLDDPDRQLLNKEFAQLIEEIERTANSTTFNEKELLNADAASATFDIHVGPGADPDNDVITLQMADLTDTGLKISELTISTKSDATTAISTLDNAIDTVSSTRATFGAYQNRLEGAIETLKIQAENLTAAESRIRDADMALEMATFTKYQILQQASTAMLAQANMSTQSILSLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K57</accession>
    <name>Q67K57_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spore cortex-lytic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2958</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SleB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2958"/>
    <dbReference type="EnsemblBacteria" id="BAD41941">
        <property type="protein sequence ID" value="BAD41941"/>
        <property type="gene ID" value="STH2958"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2958"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053345_0_0_9"/>
    <dbReference type="OMA" id="PVNPWIW"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011105">
        <property type="entry name" value="Cell_wall_hydrolase_SleB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042047">
        <property type="entry name" value="SleB_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014224">
        <property type="entry name" value="Spore_cortex_SleB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07486">
        <property type="entry name" value="Hydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02869">
        <property type="entry name" value="spore_SleB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0309">Germination</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0749">Sporulation</keyword>
    <feature type="domain" description="PG_binding_1" evidence="6">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_2" evidence="7">
        <location>
            <begin position="138"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="237" mass="25872" checksum="F15B87D36E66CE4A" modified="2004-10-11" version="1">MAQDDRHGRLERMGKRTMAAAVVALLLFLSYPWRAGAEARATLRWGSRGSDVCLAQRRLKAWGYYRGEVDCIYGRLTYEAVTLFQRRNGLTVDGIVGPDTWAALGYWGGPPSTAAAAAGTREADRDLLARVVSAEARGEPYEGQVAVAAVILNRVRDSRFPNTIAGVVYQAHAFESVTNGSVYAAPTESAVRAAQDALNGWDPSGGALFFWNPAKATSQWIWSRPIIKRIGNHVFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB2</accession>
    <name>Q67KB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3 5">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">moaC</name>
        <name evidence="8" type="ordered locus">STH2903</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3 5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197020.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2903"/>
    <dbReference type="EnsemblBacteria" id="BAD41886">
        <property type="protein sequence ID" value="BAD41886"/>
        <property type="gene ID" value="STH2903"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2903"/>
    <dbReference type="eggNOG" id="COG0315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074693_1_1_9"/>
    <dbReference type="OMA" id="IASCKVI"/>
    <dbReference type="OrthoDB" id="1595361at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01420">
        <property type="entry name" value="MoaC_PE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_B">
        <property type="entry name" value="MoaC_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="Mo_CF_biosynth-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="SSF55040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaC" evidence="7">
        <location>
            <begin position="46"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="144"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="20392" checksum="D09D284B33F573EA" modified="2004-10-11" version="1">MSNINHADAPRHGTDERGDNAQLAGQTEGGGEARLTHLDERGHARMVDVGPKPETERVAVARAEVRMKPETLTLIQSGGVPKGDVLGVARVAGIMAAKRTGDLIPLCHPLPITQASVDFAFDEAGSRVLIEARVSTVGRTGVEMEALTACAAAALTIYDMAKAVDKGMVIGEVRLVKKTGGKSGTYIRPGEDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD6</accession>
    <name>Q67KD6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flottilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family. Flotillin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196996.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2879"/>
    <dbReference type="EnsemblBacteria" id="BAD41862">
        <property type="protein sequence ID" value="BAD41862"/>
        <property type="gene ID" value="STH2879"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2879"/>
    <dbReference type="eggNOG" id="COG2268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038134_0_1_9"/>
    <dbReference type="OMA" id="ERQYDSE"/>
    <dbReference type="OrthoDB" id="534274at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031905">
        <property type="entry name" value="Flotillin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027705">
        <property type="entry name" value="Flotillin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13806">
        <property type="entry name" value="PTHR13806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15975">
        <property type="entry name" value="Flot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="5">
        <location>
            <begin position="23"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="480"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="205"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="515" mass="56225" checksum="87148A118470162D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD7</accession>
    <name>Q67KD7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2878"/>
    <dbReference type="EnsemblBacteria" id="BAD41861">
        <property type="protein sequence ID" value="BAD41861"/>
        <property type="gene ID" value="STH2878"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2878"/>
    <dbReference type="eggNOG" id="COG1585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313957_0_0_9"/>
    <dbReference type="OMA" id="IAYVRRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="217" mass="22830" checksum="F1F721300D273C55" modified="2004-10-11" version="1">MTSLFLYSFYVGFGLTLVSVILGFLNLGGDAGDLSHGGHLGPGGDLSLDAGGDLSVDLATDVPMAHDGSVGKATGKAAALSPVNFQTLVAALMGFGGAGYLTSHYGAPVALAGAAAGGFATGWLVYRFQRFLLRGERPLPPTRYTGIVGRLTVPIREGGTGEVVYTLNQTRTVSAARSVDGHPIPKGEEVVILRYERGIAYVQPWREFMKEENLKEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE6</accession>
    <name>GRDA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Glycine/sarcosine/betaine reductase complex component A</fullName>
            <ecNumber>1.21.4.2</ecNumber>
            <ecNumber>1.21.4.3</ecNumber>
            <ecNumber>1.21.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Selenoprotein PA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Thioredoxin reductase complex selenoprotein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">grdA</name>
        <name type="ordered locus">STH2869</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">In the first step of glycine, betaine and sarcosine reductases, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[thioredoxin]-disulfide + acetyl phosphate + H2O + NH4(+) = [thioredoxin]-dithiol + glycine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="EC" id="1.21.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[thioredoxin]-disulfide + acetyl phosphate + H2O + methylamine = [thioredoxin]-dithiol + H(+) + phosphate + sarcosine</text>
            <dbReference type="Rhea" id="RHEA:12825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57433"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
            <dbReference type="EC" id="1.21.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[thioredoxin]-disulfide + acetyl phosphate + H2O + trimethylamine = [thioredoxin]-dithiol + glycine betaine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11848"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17750"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58389"/>
            <dbReference type="EC" id="1.21.4.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Component of the glycine, sarcosine and betaine reductase complexes, together with components B and C.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GrdA family.</text>
    </comment>
    <dbReference type="EC" id="1.21.4.2"/>
    <dbReference type="EC" id="1.21.4.3"/>
    <dbReference type="EC" id="1.21.4.4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2869"/>
    <dbReference type="KEGG" id="sth:STH2869"/>
    <dbReference type="eggNOG" id="ENOG50313TT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142275_0_0_9"/>
    <dbReference type="OMA" id="FASTECF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00826">
        <property type="entry name" value="GRDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006812">
        <property type="entry name" value="GRDA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04723">
        <property type="entry name" value="GRDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000181">
        <property type="entry name" value="Grc_selenoprot_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <feature type="chain" id="PRO_0000249763" description="Glycine/sarcosine/betaine reductase complex component A">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="155" mass="16786" checksum="6B4BC016ADAF3A7E" modified="2008-02-26" version="2">MELKGKKAIILGDRDGIPGPALEACVRSAGAEVVFASTECFVUTSAGAMDMENQRRVKELAEKYPKEDLVVILGGAEAEASGLAAETVSNGDPAWAGPLAGVQLGLKAYHIFEPEIKEQIDPNVYEEQVSLMEMVLDVEAIVQEVRSIREKYAAW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH4</accession>
    <name>HISZ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisZ</name>
        <name type="synonym">hisS2</name>
        <name type="ordered locus">STH2839</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KH4"/>
    <dbReference type="SMR" id="Q67KH4"/>
    <dbReference type="STRING" id="292459.STH2839"/>
    <dbReference type="EnsemblBacteria" id="BAD41824">
        <property type="protein sequence ID" value="BAD41824"/>
        <property type="gene ID" value="STH2839"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2839"/>
    <dbReference type="eggNOG" id="COG0124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_0_0_9"/>
    <dbReference type="OMA" id="ELVMPPM"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242860" description="ATP phosphoribosyltransferase regulatory subunit">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00125"/>
        </source>
    </evidence>
    <sequence length="399" mass="42441" checksum="0372C1233169CC92" modified="2004-10-11" version="1">MSTPVTRTQVPDGVRDRLPSEAAAMRRLSARLDNVFRAWGYREVSTPVIEYLEAVAAGAANWGRREDLYQFFDRKGRTLALRPDMTTPIARLMATRLADEPLPLRLSYFAPVFRHRELRAGATSEIWQAGVELVGAPGEAADAEVISLACAAVRAADLTGFRIGLGHVGVVEGLFESAGVDPQAAAVLKEAMVARDLVAFEQGVARAGLSGERAERLLALVHFHGSYAEAVARFGGVGGRVAEALQHLGRVLEVLEALGVAEQVNLDLGLVRSLGYYTGVVFEGYLPGIGAPVLGGGRYDNLVAEFGRPLAATGFALEVDRLLMAQEQQGALAAEPGLDAVIACPPGQEAAAMAWAARLRAQGLAVEVDFLDRTGEELAAYARARAAARVLRPGVPSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN0</accession>
    <name>Q67KN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196902.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2783"/>
    <dbReference type="EnsemblBacteria" id="BAD41768">
        <property type="protein sequence ID" value="BAD41768"/>
        <property type="gene ID" value="STH2783"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2783"/>
    <dbReference type="eggNOG" id="COG1728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121413_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005585">
        <property type="entry name" value="DUF327"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024042">
        <property type="entry name" value="TM1646-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03885">
        <property type="entry name" value="DUF327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158397">
        <property type="entry name" value="SSF158397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="17648" checksum="785C3EB21ACABB33" modified="2004-10-11" version="1">MERVRLITGLTRIATTGHGERATPATRVPFREQLARSHAMQFNERIDRALQEIDELGRRLTESLSQHDLQLYREAIGRLFRDLTHHMMEVRQNLEWDSYAWEQRTMVTIQRVDQRLEELARMVLEQEQDRLAILAAVDEIRGLLLDVRM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-12-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP3</accession>
    <name>NUOK2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit K 2</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit K 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit K 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoK2</name>
        <name type="ordered locus">STH2770</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KP3"/>
    <dbReference type="SMR" id="Q67KP3"/>
    <dbReference type="STRING" id="292459.STH2770"/>
    <dbReference type="EnsemblBacteria" id="BAD41755">
        <property type="protein sequence ID" value="BAD41755"/>
        <property type="gene ID" value="STH2770"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2770"/>
    <dbReference type="eggNOG" id="COG0713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_1_1_9"/>
    <dbReference type="OMA" id="NFVAFSY"/>
    <dbReference type="OrthoDB" id="2081663at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="PTHR11434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000390259" description="NADH-quinone oxidoreductase subunit K 2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01456"/>
        </source>
    </evidence>
    <sequence length="107" mass="11700" checksum="089AD5B260E223AB" modified="2004-10-11" version="1">MELPIYYPLGLGALLFGLGLWGALTQKNAVRILMFIEIMLNGVNLNLITFSRYYWQTSPEMAARAPILTLFVMTVAAAEASVGLAIILAMVRNRGVVEVDKATLLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS0</accession>
    <name>Q67KS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2742</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2742"/>
    <dbReference type="EnsemblBacteria" id="BAD41727">
        <property type="protein sequence ID" value="BAD41727"/>
        <property type="gene ID" value="STH2742"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2742"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_2_9"/>
    <dbReference type="OMA" id="TSGKWAD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="411" mass="44218" checksum="20101D55C4DB2ADC" modified="2004-10-11" version="1">MEVGVMHEERGVLALLRHRNYMLYWFGFLVSNAGAWIQSVAQGWLVYDLSNSAAWLGTVGFVRAFPLIFLSLIGGTVADRLPKRRILYVTQTVQMISAALLGTLTLLGHIQVWHVVLLSAVSACAQAFDQPARHALVPHLVPRHNLHAAISFNSIAFNGAALFGPSLTGVLVPLIGFAGCFYVNAVSFVAVFIALALMEFPPHAPGGGKTSMLEDLRAGLSFIGHNQVILALISMAAVTSFFARPYQQFITVFARDVVHGDVDTAGLMQAAPALGTVIFMLAIASATNIQWKGKLLLGAGILFPASLIAFSWSTNLYVSLALLVLVGGLNMTWQTTLNTLLQTNVGDEMRGRVMSAYTMTALAMMPLGQGPMGMAFDYLGPSLALTLNALIALAWTVYMGLIRVKAIRTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS7</accession>
    <name>Q67KS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196854.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2735"/>
    <dbReference type="EnsemblBacteria" id="BAD41720">
        <property type="protein sequence ID" value="BAD41720"/>
        <property type="gene ID" value="STH2735"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2735"/>
    <dbReference type="HOGENOM" id="CLU_1634532_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038144">
        <property type="entry name" value="IPI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269250" evidence="1">
        <location>
            <begin position="25"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="18086" checksum="206114ED24F62F23" modified="2004-10-11" version="1">MRRVLFPVLVVLALALSLAGCGRAQGPQWGNLNLTLEDVQQNENHWTARMVLSNPTDKVQVIEYTADARYTMIVTRNGQTVLEQPFDILRDGDPRILNLSPGVSKEYVVAWTYRNKEGERVEPGTYEVSVRLDAVTVVQEAGKTQPTVIEPRTVGPVKVQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW6</accession>
    <name>Q67KW6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2695</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2695"/>
    <dbReference type="EnsemblBacteria" id="BAD41680">
        <property type="protein sequence ID" value="BAD41680"/>
        <property type="gene ID" value="STH2695"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2695"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_57_3_9"/>
    <dbReference type="OMA" id="GYINNAN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="43294" checksum="2FAC31D5C289A6C6" modified="2004-10-11" version="1">MLWVGNFLTACSFSLVMPFLPRFIAELGVTANLYTWSGLIFSVTFLSSSIMAPIWGNLADRYGRRPMLIRAGVAISIVYLLMSFVTSHVQLFWLRLLNGAFSGFIPSAVALVATNTPEDRVGRYLAILQTGMATGTILGPLLGGTLAELLGIRWTMRVASVLVLISVLLVIFLVRERVLGAGKARTSVLSDLKLAVSNRRLLALMISALLIQASLQSLQPILSNFIPTLTREGWIAAVTRSLFGEGEATDFVVGFVFSLPAIATVLTAPRLARAGERIGFARLLSAGLLAAGLLILPQSLVQSIGWLILLRFVFGIATAAVQPSVNAALAEVVHPSFRGRAYGINTSANNLGSVLGPSLGGWVADAFGPRAVFVVTGLVMLAASAWVQRMLVHGPEEPLVGAPEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY1</accession>
    <name>Q67KY1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CoA_binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2680"/>
    <dbReference type="EnsemblBacteria" id="BAD41665">
        <property type="protein sequence ID" value="BAD41665"/>
        <property type="gene ID" value="STH2680"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2680"/>
    <dbReference type="eggNOG" id="COG1832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112567_0_0_9"/>
    <dbReference type="OMA" id="MVMDRCP"/>
    <dbReference type="OrthoDB" id="1910664at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA_binding" evidence="1">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="138" mass="15291" checksum="1E1635B96C6AE867" modified="2004-10-11" version="1">MAVNDPKTVAWVLDGPKVLAVVGLSDNPERPSYRVSAEMQRRGYRIVPVNPKGGEILGEKVYSSLREIPFPVDVVQVFRAPQYALDVVREIGEMVARPRVLWMQEGVVNDEAVAEAEALGLLTVQDRCLYKEALKAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L23</accession>
    <name>Q67L23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Succinate dehydrogenase membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2638</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2638"/>
    <dbReference type="EnsemblBacteria" id="BAD41623">
        <property type="protein sequence ID" value="BAD41623"/>
        <property type="gene ID" value="STH2638"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2638"/>
    <dbReference type="eggNOG" id="COG2142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127125_0_0_9"/>
    <dbReference type="OMA" id="WLFMRLS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12404" checksum="7F5BE264DB8D2486" modified="2004-10-11" version="1">MNQVKGSLWAWILHRITAVLLVVLVAIHFGIMHFVDPTAVITFADSQMRLQSALYLVVDSGLLVLGLYHGLNGIRNVVLDYWPRSGRVVGWILAVIGVVAAGYGSMALAAFLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L45</accession>
    <name>Q67L45_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper-exporting P-type ATPase</fullName>
            <ecNumber evidence="3">7.2.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Copper-exporting P-type ATPase A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Cu(+)-exporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">STH2616</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + Cu(+)(in) + H2O = ADP + Cu(+)(out) + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25792"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.8" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2616"/>
    <dbReference type="EnsemblBacteria" id="BAD41601">
        <property type="protein sequence ID" value="BAD41601"/>
        <property type="gene ID" value="STH2616"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2616"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_9"/>
    <dbReference type="OMA" id="ITFFGWM"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140581">
        <property type="term" value="F:P-type monovalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006122">
        <property type="entry name" value="HMA_Cu_ion-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00003">
        <property type="entry name" value="TIGR00003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-0187">Copper transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="759"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="784"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="816"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="16">
        <location>
            <begin position="18"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="16">
        <location>
            <begin position="86"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD41601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="949" mass="100541" checksum="14E33E2FA8586135" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L80</accession>
    <name>Q67L80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2581"/>
    <dbReference type="EnsemblBacteria" id="BAD41566">
        <property type="protein sequence ID" value="BAD41566"/>
        <property type="gene ID" value="STH2581"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2581"/>
    <dbReference type="HOGENOM" id="CLU_507061_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="537" mass="56676" checksum="42A688093BA0EEDD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI4</accession>
    <name>Q67LI4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA dehydrogenase, short-chain specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2477</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2477"/>
    <dbReference type="EnsemblBacteria" id="BAD41462">
        <property type="protein sequence ID" value="BAD41462"/>
        <property type="gene ID" value="STH2477"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2477"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_2_9"/>
    <dbReference type="OMA" id="NTHDVVY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013107">
        <property type="entry name" value="Acyl-CoA_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08028">
        <property type="entry name" value="Acyl-CoA_dh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="5">
        <location>
            <begin position="53"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="4">
        <location>
            <begin position="160"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_2" evidence="6">
        <location>
            <begin position="281"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="434" mass="48112" checksum="2D967C61C2392D41" modified="2004-10-11" version="1">MKRMQGRTKNPGMLQEFRRAAAKLSTTEVNPVTLGPWFFGQTEVDWQAYEMADDLADRFAQTAAEYDRTGTFPFAHFEAMRERGYLALTVPREDGGLGAGVHAMVFAQERLARGDGSTALAVGWHLYVVGKQAHSRTWPEPVRRRLFGEVVTDGALLNACASEPETGSPSRGGLPRTTARRLPDGSWVLTGRKNFTTLSPILRYFLVSATLDGTEEAAWFLVPRDAPGLHVEETWDSLGMRATGSHDLRLEEVHLPADALLESARERTEGRTEGWLLHVPAVYLGVAQAARDFAVKYAVERRTVNPPGHPEPTSRLADRPHVQRLIGEMDLALLPARHLLMGLARRWDERPEWRSRMVDAVAACKPFVVETALRVVDQAMRVAGGASLARSLPLERFYRDARAGLHNPPMEDVVIGNLARSAIRRAEAGEDAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL4</accession>
    <name>Q67LL4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2447"/>
    <dbReference type="EnsemblBacteria" id="BAD41432">
        <property type="protein sequence ID" value="BAD41432"/>
        <property type="gene ID" value="STH2447"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2447"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102167_0_0_9"/>
    <dbReference type="OMA" id="HDYLYTY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039248">
        <property type="entry name" value="Ptase_RsbX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35801">
        <property type="entry name" value="PTHR35801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07228">
        <property type="entry name" value="SpoIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="25285" checksum="2252390E86CF244B" modified="2004-10-11" version="1">MQVTMAVAKVPKFGLVESGDSVELLERPRGGLTAILADGQRHGRSAKRLSQLVVAKAMQLVADGVRDGAVARGVHDYLYAVRDGKVSTELVLLSVDARTGTLVVTRNTHVPVLVRRGDGSVLRLDEPVEPIGMRETLRPVIAEIPLEPGLVALAMTDGVYAAGRWHGRQWPVEALEAIVREADPASMQALADRVLAEAMRLDEQRPADDMAVLAVGVTAGGEDRIRRMSVTMEW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM3</accession>
    <name>APT_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="1">APRT</shortName>
            <ecNumber evidence="1">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apt</name>
        <name type="ordered locus">STH2438</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196561.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LM3"/>
    <dbReference type="SMR" id="Q67LM3"/>
    <dbReference type="STRING" id="292459.STH2438"/>
    <dbReference type="EnsemblBacteria" id="BAD41423">
        <property type="protein sequence ID" value="BAD41423"/>
        <property type="gene ID" value="STH2438"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2438"/>
    <dbReference type="eggNOG" id="COG0503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_3_0_9"/>
    <dbReference type="OMA" id="KPGIVFR"/>
    <dbReference type="OrthoDB" id="1532478at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149473" description="Adenine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <sequence length="170" mass="18653" checksum="0B906914FBB8CCD7" modified="2004-10-11" version="1">MDFKSKIRTVDDFPKPGISFKDITTLLKDGEAFHAAIETMAAHFEPLGINMVTGPEARGYIFASALAFRLRAGFVPIRKPGKLPWKTRSISYQLEYGEDRLEVHEDAFQPGQKVLVVDDLLATGGTIRATIDLVESLGAKVVGVAVLIELSDLGGRKRLEGHDVISLVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ5</accession>
    <name>Q67LQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2406"/>
    <dbReference type="EnsemblBacteria" id="BAD41391">
        <property type="protein sequence ID" value="BAD41391"/>
        <property type="gene ID" value="STH2406"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2406"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2095637_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="13039" checksum="46F1E8FF17DF8548" modified="2004-10-11" version="1">MYRSLLDLMLSPPWPAPDDGSQLTPQWGEDERFLTLRFRTAGLDPKSIRVEVGPTAVSVSGYRTHEERVEARGYFRASSSMSAVRRTFGLPCLVVPGAASVRWRSDDELEVRLQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT1</accession>
    <name>Q67LT1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2380"/>
    <dbReference type="EnsemblBacteria" id="BAD41365">
        <property type="protein sequence ID" value="BAD41365"/>
        <property type="gene ID" value="STH2380"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2380"/>
    <dbReference type="HOGENOM" id="CLU_1730490_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="16640" checksum="6629C218762CBE06" modified="2004-10-11" version="1">MPKRFTACPQEWAASPSRPMDGICCTPPAISAIAGRRTAPWCLTSRPWRLLRAPTWFCSGSVNFNWGDFGRMARCDVTVERIPALIDAWGQASGANPTGKMVISVTDPNREDVVARSVGSNQTVSFNVGNHGVFGTYEIRYSTPHSHKWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT9</accession>
    <name>Q67LT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2372"/>
    <dbReference type="EnsemblBacteria" id="BAD41357">
        <property type="protein sequence ID" value="BAD41357"/>
        <property type="gene ID" value="STH2372"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2372"/>
    <dbReference type="HOGENOM" id="CLU_2902615_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="62" mass="6987" checksum="561ADAF7777ECE04" modified="2004-10-11" version="1">MKPVWVHPQTLEYMGRSIVEKVYAVKVPKGLRGVRRAGRQPGRALSRRRAHHAGVHSQPVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW8</accession>
    <name>Q67LW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2343</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196466.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2343"/>
    <dbReference type="EnsemblBacteria" id="BAD41328">
        <property type="protein sequence ID" value="BAD41328"/>
        <property type="gene ID" value="STH2343"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2343"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_9"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="37586" checksum="D1F2BA74ABDA2550" modified="2004-10-11" version="1">MKKRSVSIKEISELAGVSVATVSRVINRNGRYSKETEQRVLQIIEEYGYQPDLVARGLRTRKLQVVGVIVPDITNEFFARVTQELQNNLFALGYSTIICNTNEEYEKEKRYLAMLRAQKVSGLIYISGLRTEGQPSLLVPTVYIDRKPPRTSETAELVFIESANEEGGYLATRSLLDSGCRRVGLITYRPDISSHGDRLRGYRKALEERGLPFDQDLVVQVSRVSMSSGFEAALQLMRAHPEIDGFFCTADLLAYGVLSCLSGPAGMAAGRRIRLVGFDDLSFSGTEAFSLSSVRQSVEEFGRLGAYAVVQMIEGKPLERKHYRLPVELVLRNTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX9</accession>
    <name>Q67LX9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Anaerobic dimethyl sulfoxide reductase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dmsA1</name>
        <name evidence="10" type="ordered locus">STH2332</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196455.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2332"/>
    <dbReference type="EnsemblBacteria" id="BAD41317">
        <property type="protein sequence ID" value="BAD41317"/>
        <property type="gene ID" value="STH2332"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2332"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_9"/>
    <dbReference type="OMA" id="ANGEQSM"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009389">
        <property type="term" value="F:dimethyl sulfoxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011888">
        <property type="entry name" value="Anaer_DMSO_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10518">
        <property type="entry name" value="TAT_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02166">
        <property type="entry name" value="dmsA_ynfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="9">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="792" mass="86830" checksum="B23AEBEB01923EAE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ2</accession>
    <name>Q67LZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196442.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2319"/>
    <dbReference type="EnsemblBacteria" id="BAD41304">
        <property type="protein sequence ID" value="BAD41304"/>
        <property type="gene ID" value="STH2319"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2319"/>
    <dbReference type="eggNOG" id="COG3214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043035_0_1_9"/>
    <dbReference type="OMA" id="FEYWAHE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009351">
        <property type="entry name" value="YcaQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06224">
        <property type="entry name" value="HTH_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="474" mass="53333" checksum="54A0302813B25B84" modified="2004-10-11" version="1">MRVRVSKAAARRFLLYAQGLLEVPGVIQPWAKELRGRSGLLRALERLEAVQLDPIAVVERNHHLVLRNRVGGYRPEHLDGLFAAGQVFEYLANARCVLPMRDLPDFWPIMLAARQLPARAALADVMTDVLRRLYREGPLPPRALGNDGPRLLGLGYNPPDKASKASGRAIDLLWLGGEVFVARREGNEKWYDLAERVIPRNVAADLPGDFSNLRSPMLRAHAGVGAEGQETGPERAGDSAHGAGARGEGGGGEGERRALPGDSPVLTGVDVSRPWSTPDVGAGEAEWQRFLLDKYMRAYRLVDLGDFRFGWQSYPSARRRALAAEKVAKGEWVEVEVDGVRRRYFALAEDRPCLEVAGDWEPRRTVRFLAPLDNLLWRRERLQDLFDFDYTWEVYTPAAKRRFGYYAMPILYGDRLIGRLDPKLDRENGVLVVNRLQLELDGVDRRGLETSLEKALYAFARWHGAEDVRILQRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-19" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M16</accession>
    <name>RL252_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L25 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">General stress protein CTC 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplY2</name>
        <name evidence="1" type="synonym">ctc2</name>
        <name type="ordered locus">STH2294</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196417.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67M16"/>
    <dbReference type="SMR" id="Q67M16"/>
    <dbReference type="STRING" id="292459.STH2294"/>
    <dbReference type="EnsemblBacteria" id="BAD41279">
        <property type="protein sequence ID" value="BAD41279"/>
        <property type="gene ID" value="STH2294"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2294"/>
    <dbReference type="eggNOG" id="COG1825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075939_2_0_9"/>
    <dbReference type="OMA" id="KEVQTHY"/>
    <dbReference type="OrthoDB" id="1673077at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000181605" description="50S ribosomal protein L25 2">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <sequence length="194" mass="20874" checksum="6FED874E41CF8BB1" modified="2004-10-11" version="1">MQLEATPRGTGSRASRRLRQAGFVPGIIYGPGVEPLAVSVRSTQLERLVERQGRGHLIHVQVEGEANPRQVVIKQLQRDILTQQVTHVDFLQVDMNRTITLTVPIVVVGEEQARRRGLLITHELDEVEVECRPTEIPEAVTLDVSGLTEPGPVTAASLSAPPGVRVLEDPDTVVVSCTVIGGGEEEEASTGPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M50</accession>
    <name>Q67M50_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196384.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2260"/>
    <dbReference type="EnsemblBacteria" id="BAD41245">
        <property type="protein sequence ID" value="BAD41245"/>
        <property type="gene ID" value="STH2260"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2260"/>
    <dbReference type="eggNOG" id="COG1630">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_716910_0_0_9"/>
    <dbReference type="OMA" id="RWDEYAR"/>
    <dbReference type="OrthoDB" id="577691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="43610" checksum="867EB44C338F44F4" modified="2004-10-11" version="1">MLHPDALTGIKEQIIRLAEADRALLDDLRREVRDLAGSVRVIRPRSSTAISLVASDGGNNKLVFDPFYVQVVRVVDSYGKELLVDVVSPTTDPDSLLTNHFTPSGDPKSALGRLMADLGVRSLSALSHMIPDSRRVRERPETVSPSWVQVYRDICEWAVLYERICYQTFASDTLIVRDGLLRSKVFRGEGFIRMRERMEEAIERVWRQDRRRIYLVGLAKHSQVLNRYGLAMAIEETLPAGDARYVAIPRELEAKAYKWQEWARGAETEEGEAPKFVAGDMFFVRFGPHSGDPVWAVDIFSAQRDRADEILGHLLADARDGFPVPYYPRCLQRAHEHAQLADFDWAILQDQILDAVRGLLPREKQFLLDAYRLRADVTGERYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA2</accession>
    <name>Q67MA2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">XdhC_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2206"/>
    <dbReference type="EnsemblBacteria" id="BAD41191">
        <property type="protein sequence ID" value="BAD41191"/>
        <property type="gene ID" value="STH2206"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2206"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_3_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_C" evidence="1">
        <location>
            <begin position="98"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="27773" checksum="7ED784F9F7ABC4A1" modified="2004-10-11" version="1">MRHMSYLNALAQEIAAERPVAVAKVVEGPWAGCAVVAGPGGIHYVDPRLQAAPEGLAEGMAELAAGLLVRGESGCHRLRLPAGSVRVYVESHLPPPVLLVVGAGHVGREVARAGALAGFQVWVVDDRREYAHPAHFPTAYRVICVPLPEELAALAPGERHHVVLVTRGHAQDAACLAALAGAPVPYVGMIGSRKRVGAVFDALRAGGVDEEWLGRVRAPIGLDIGARTPGEIAIAVVAEVIATRRGGTGKPLSALDRPLIHLSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB5</accession>
    <name>Q67MB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196318.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2193"/>
    <dbReference type="EnsemblBacteria" id="BAD41178">
        <property type="protein sequence ID" value="BAD41178"/>
        <property type="gene ID" value="STH2193"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2193"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="QITNIFH"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="30316" checksum="7008F3E65371C849" modified="2004-10-11" version="1">MEPEIILETVDVAKSFGGLAANAGISIKVPARTLRTVIGPNGAGKTTLFNLISGIYKPTAGRILFRGEDITGLPPHRIARKGIGRSFQITNLFPELTVLENVRLAAQARGRHSWHLWRSAEALREYAEKAEEALRTVGLLHRARALARELAHGEKRKLEIAILLATDPVVMLLDEPTAGMSREEVPGIIEVIERIKAQRSRTILMVEHKMDIVLGISDAITVLAQGEVIAEGTPAEIVANEAVQAAYLGTTHGYTLAGSRGRQGADPGREAPDIPAAQARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC8</accession>
    <name>Q67MC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycerol-3-phosphate ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2180"/>
    <dbReference type="EnsemblBacteria" id="BAD41165">
        <property type="protein sequence ID" value="BAD41165"/>
        <property type="gene ID" value="STH2180"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2180"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_9"/>
    <dbReference type="OMA" id="IAFFIAW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="71"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="30712" checksum="C1195F40F46EE681" modified="2004-10-11" version="1">MSVQSRLTRLLLYAALCLAALFMVFPILYAVSISLMPPAEVNTFPPRFLPSSPRWANYAEVARSVPVGRYLLNSLVVSSAVTLGQIATASLSAYAFAFMNFRGKAALFALFMSTMMVPWEVTLIPNYLTIRSLRLLDSYTGLVLPFLATAFGTFLLRQFFLQIPRELEEAARIDGCGRFRFFWSIALPLARPGLLTLGAYTFLSTWNQYLWPLLVTNTRLMRTVQIGVRFLMNEEGLQYHRVMAGVVLCMAPALVVLVLGQRYLVRGLMAGGLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME7</accession>
    <name>Q67ME7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched-chain alpha-keto acid dehydrogenase E1 beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196286.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2161"/>
    <dbReference type="EnsemblBacteria" id="BAD41146">
        <property type="protein sequence ID" value="BAD41146"/>
        <property type="gene ID" value="STH2161"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2161"/>
    <dbReference type="eggNOG" id="COG0022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_9"/>
    <dbReference type="OMA" id="SEAYYMA"/>
    <dbReference type="OrthoDB" id="900125at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="327" mass="35480" checksum="CA006717F8350E64" modified="2004-10-11" version="1">MPVMNLVEAVRDALRTEMRRDPDVWIVGEDVGKKGGVFGATLGLYDEFGPQRVMDSPLTESAIVGVGIGAALYGTKPVCEIQFADFIFPAMNQIVSEAAKMRYRSNSAWTVPMVIRAPFGGGVHGGLYHSQSVEQYFTNTAGLKVVVPSTPYDAKGLLISAIRDPDPVLFFEHKGLYRAVKGEVPEGDYTIPIGKAEVKRDGTDITVITYGKVVHFCLEAAELLAREGYEALVLDLRTLLPLDREAIVAAARKTGKVLIAHEAGKTHGVGAEVAAIIAEECLFDLDAPIKRLCGPDVPAMPYAGPMEKFYMLSTEKCLAAMRELAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI7</accession>
    <name>Q67MI7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transketolase</fullName>
            <ecNumber evidence="4">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2121</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2121"/>
    <dbReference type="EnsemblBacteria" id="BAD41106">
        <property type="protein sequence ID" value="BAD41106"/>
        <property type="gene ID" value="STH2121"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2121"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_9"/>
    <dbReference type="OMA" id="HHTEGIE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transket_pyr" evidence="9">
        <location>
            <begin position="334"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="650" mass="71308" checksum="DE29C3409D5844D8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR9</accession>
    <name>Q67MR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Biphenyl dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2039</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196165.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2039"/>
    <dbReference type="EnsemblBacteria" id="BAD41024">
        <property type="protein sequence ID" value="BAD41024"/>
        <property type="gene ID" value="STH2039"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2039"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_2_9"/>
    <dbReference type="OMA" id="TLECWLH"/>
    <dbReference type="OrthoDB" id="1681886at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="4">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="101" mass="10863" checksum="2D1A43AA4648BE9B" modified="2004-10-11" version="1">MALVKVAELGEIPPGGRKRVLVKRHPIALFHLEDGFYAIDDTCTHAEASLCEGQLMGDQVACPLHGARFDIRTGKALTLPAVTPVTTYPVVVQGNDILVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N03</accession>
    <name>Q67N03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196082.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1955"/>
    <dbReference type="EnsemblBacteria" id="BAD40940">
        <property type="protein sequence ID" value="BAD40940"/>
        <property type="gene ID" value="STH1955"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1955"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_608231_0_0_9"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="78"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="450" mass="48885" checksum="A6752D2A390C9D0A" modified="2004-10-11" version="1">MSAAQKGSRLPLIAGGLLVGLLLLAAVFADEITAVSPHYWDARSSILEGRPPFPPGPGHPLGTDEWGRDVWSRVVYGTRWSLLFAALVTAGRMLIGLAATFAAVYGPRRLGWLVDKLYVMTTAIPPLVTYLLLLSSPVKNTIGLWPNVALTACVLTLVEWPRVAVTLKGRLQQLMAEPFVEGAVAVGGSRWHVFHIHLLPHLWPTLLHLVAAEMARALSVIAQMGIFGILFGGGIMVVGDARNPDRWYQVSGLPEWGSLLSDGRLQVLSRPWIAFPPAVAFLIAVTGFTLLSQGLEGLNLPVARIKERTTGRLSRRWRWALAALPLFGLLWYYQGLPWDRAAGIQALATRQAAALSAGDAEGYAGTVAPGSDALRADARLLAAALAGERVESVDVAMEEVRLRGARAQARLTMTVTFADSPPLHLSRPVDLVRRLGTWYVEDRELYTLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N78</accession>
    <name>Q67N78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1880</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1880"/>
    <dbReference type="EnsemblBacteria" id="BAD40865">
        <property type="protein sequence ID" value="BAD40865"/>
        <property type="gene ID" value="STH1880"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1880"/>
    <dbReference type="eggNOG" id="COG1121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_9"/>
    <dbReference type="OMA" id="FYFDPFT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="250" mass="27795" checksum="2DA2744A86F3FDA7" modified="2004-10-11" version="1">MRNVSFGYGKDLVLDRVSLTVGRGEYLGLIGPNGSGKTTLLRLILGLLPLTQGEIRLFGTPVQQFRERWRVGYVAQKAASFNSAFPATVEEVVSTGLTARRGLFRRFTASDRLAVRQALDRVGLLPYKDRLVGRLSGGQQQRVFIARALVSRPELLILDEPTVGVDASAEGQFYRLIRRLREEMGLTVILVSHDIGAITHEVSALACLNRRLFYHGSPADFGTEEQLCELYGHDVLRIHHHHHRGEGDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA3</accession>
    <name>Q67NA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage III sporulation protein AH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoIIIAH</name>
        <name evidence="2" type="ordered locus">STH1855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195983.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1855"/>
    <dbReference type="EnsemblBacteria" id="BAD40840">
        <property type="protein sequence ID" value="BAD40840"/>
        <property type="gene ID" value="STH1855"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1855"/>
    <dbReference type="eggNOG" id="ENOG5032YS3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1395691_0_0_9"/>
    <dbReference type="OMA" id="PTHESMP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024232">
        <property type="entry name" value="SpoIIIAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038503">
        <property type="entry name" value="SpoIIIAH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12685">
        <property type="entry name" value="SpoIIIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="21241" checksum="25FEDC26F5BB04FB" modified="2004-10-11" version="1">MFVVKRGSDLLRFALYVLVVASLVWYVVSRFGEWRAAQAPSRGPVLADGPAPVVGPEPEEAEEALVRPDEEVLAEGAEGTDYFAEYRIERERTRGALGDRLREVMVAEGASAEVRQEAAAQYLELGRRAALESQAEALVRARGFTDVIVHLTDGSAQVVVKARSLSQQQVAQIIDTVSRTTGVRATAITVMARDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK3</accession>
    <name>Q67NK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1755"/>
    <dbReference type="EnsemblBacteria" id="BAD40740">
        <property type="protein sequence ID" value="BAD40740"/>
        <property type="gene ID" value="STH1755"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1755"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_7_1_9"/>
    <dbReference type="OMA" id="LMAMDPI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="30871" checksum="97B448BA1A60125B" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLEGQRGEERSGPAVEAAVPRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL2</accession>
    <name>HFQ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein Hfq</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hfq</name>
        <name type="ordered locus">STH1746</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Hfq family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195874.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NL2"/>
    <dbReference type="SMR" id="Q67NL2"/>
    <dbReference type="STRING" id="292459.STH1746"/>
    <dbReference type="EnsemblBacteria" id="BAD40731">
        <property type="protein sequence ID" value="BAD40731"/>
        <property type="gene ID" value="STH1746"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1746"/>
    <dbReference type="eggNOG" id="COG1923">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113688_3_0_9"/>
    <dbReference type="OMA" id="QQMVYKH"/>
    <dbReference type="OrthoDB" id="1989988at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01716">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00436">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34772">
        <property type="entry name" value="PTHR34772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02383">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000095663" description="RNA-binding protein Hfq">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00436"/>
        </source>
    </evidence>
    <sequence length="90" mass="9939" checksum="28EFB18D111E51AA" modified="2004-10-11" version="1">MTKASASLQDGFLNLLRRENIPATIYLVNGYQLKGYIRGFDNFTVAVEVDGRVQLVYKHALSTITPARPLPVSVSQIMRAGEGQEVEGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT8</accession>
    <name>Q67NT8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">30S ribosomal protein S1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1670"/>
    <dbReference type="EnsemblBacteria" id="BAD40655">
        <property type="protein sequence ID" value="BAD40655"/>
        <property type="gene ID" value="STH1670"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1670"/>
    <dbReference type="eggNOG" id="COG0539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015805_4_0_9"/>
    <dbReference type="OrthoDB" id="1235756at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="56"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="145"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="231"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="316"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="441" mass="48243" checksum="EBEE7050B1DD5BBF" modified="2004-10-11" version="1">MHGSSRSRRCRTWSEPSRRSTIVQEFDTQGRKNVETMEEAEARAELEAALRVPKPGDIVRGQVVRVSDDSIMVDVGYKSEGIIPLNELSHRPLRSAHDAGLKVGDEVQVVVLSVDTKGEGGLRISKRRADEQLAWAEVERKFEAGEVIEAEVTEAVKGGLVVDLGLRAFLPASQVDRGYVADLSKFVGQKIRVKIIELDPYKGRVILSRKQVLEAERTAARERFWSTVQEGDVLEGTVKSITDFGAFIDLGGVDGLLHISEMSYGRIKHPSQVVSEGERIKVKVLRLDREKGKVSLGLKQVLPDPWDSVEEKYPEGAIVEGTVARLTTFGAFVELEPGVDGLIHISQMADRRINNPGEVVSIGDVVKVKVINVDAQNRRISLSLRAAIEELAAEAIAADVATPYRDVVPAEAGEAPSGAEQQDEPAREAEESGSAEETQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU8</accession>
    <name>Q67NU8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1660</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit F family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1660"/>
    <dbReference type="EnsemblBacteria" id="BAD40645">
        <property type="protein sequence ID" value="BAD40645"/>
        <property type="gene ID" value="STH1660"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1660"/>
    <dbReference type="eggNOG" id="COG2212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125825_1_3_9"/>
    <dbReference type="OMA" id="ARYMERG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007208">
        <property type="entry name" value="MrpF/PhaF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34702">
        <property type="entry name" value="PTHR34702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04066">
        <property type="entry name" value="MrpF_PhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="11943" checksum="4895F2214EA0BBC9" modified="2004-10-11" version="1">MLQAVLWISLVGMVVSLGFAAYRTFRGPTVPDRIAAIDAFGTVLIQTLIVGAILLNDPQYLAYAVVLAAINYVSTVALGKYLQRGGVIGHGDPHRPVGPAGDRGGGAGDAGARPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P33</accession>
    <name>Q67P33_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195704.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1575"/>
    <dbReference type="EnsemblBacteria" id="BAD40560">
        <property type="protein sequence ID" value="BAD40560"/>
        <property type="gene ID" value="STH1575"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1575"/>
    <dbReference type="eggNOG" id="ENOG5032S8H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142305_0_0_9"/>
    <dbReference type="OMA" id="ALNFVMY"/>
    <dbReference type="OrthoDB" id="1787530at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="11787" checksum="BE124C07BDF3DC4E" modified="2004-10-11" version="1">MARRIRLIDICHARSGDKGDASDISLFANDDAAWEIIRKHVTKERVAEYFSPVATGPVERWEVPNVQALKFVVHGALGGGAPRSLRSDNLGKTFAAALLRMEITVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P38</accession>
    <name>Q67P38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1570"/>
    <dbReference type="EnsemblBacteria" id="BAD40555">
        <property type="protein sequence ID" value="BAD40555"/>
        <property type="gene ID" value="STH1570"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1570"/>
    <dbReference type="HOGENOM" id="CLU_511828_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="532" mass="54022" checksum="038E7DFD7B861F50" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P77</accession>
    <name>PNP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide phosphorylase</fullName>
            <shortName evidence="1">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pnp</name>
        <name type="ordered locus">STH1531</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195661.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P77"/>
    <dbReference type="SMR" id="Q67P77"/>
    <dbReference type="STRING" id="292459.STH1531"/>
    <dbReference type="PRIDE" id="Q67P77"/>
    <dbReference type="EnsemblBacteria" id="BAD40516">
        <property type="protein sequence ID" value="BAD40516"/>
        <property type="gene ID" value="STH1531"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1531"/>
    <dbReference type="eggNOG" id="COG1185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004217_2_2_9"/>
    <dbReference type="OMA" id="LHILDVM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="SSF46915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000329893" description="Polyribonucleotide nucleotidyltransferase">
        <location>
            <begin position="1"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="569"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="643"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="711"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="762" mass="82820" checksum="09DA829CDDDAF97C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P84</accession>
    <name>Q67P84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exopolyphosphatase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1524"/>
    <dbReference type="EnsemblBacteria" id="BAD40509">
        <property type="protein sequence ID" value="BAD40509"/>
        <property type="gene ID" value="STH1524"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1524"/>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039720_0_0_9"/>
    <dbReference type="OMA" id="CTRWVES"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="20"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="238"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="324" mass="34934" checksum="20F2460A0EF6EF72" modified="2004-10-11" version="1">MSKLRVDLQAASRMIAGAQRILFFMHVQPDGDSIGSTLGMIRALRQMGKEAIMVGADPIPPVFHFLPGWDEYCVPWQEVEGEWDLSCFLDCGDLERVGPALPAVRRGRTTLNVDHHATNTAFAEYNYLDFSAAAVGELAYRILREIGAPIDAGTALCLYTSLVTDTGGFRYDSTGPGTHRVAADLIELGARPYDVAQAVFETESLSRLALLSRALQTLQLDETGRVAWMVVTRKLLAEAGATDHDVEGVVNYARSVAGVEVGILFKETEDGGVRVNLRSRRTVDVGAIAQRFGGGGHARAAGCTLNVPMEDAVAQVMAAVKVVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA6</accession>
    <name>SYP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">STH1502</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195632.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PA6"/>
    <dbReference type="SMR" id="Q67PA6"/>
    <dbReference type="STRING" id="292459.STH1502"/>
    <dbReference type="EnsemblBacteria" id="BAD40487">
        <property type="protein sequence ID" value="BAD40487"/>
        <property type="gene ID" value="STH1502"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1502"/>
    <dbReference type="eggNOG" id="COG0441">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_9"/>
    <dbReference type="OMA" id="EVYWVTH"/>
    <dbReference type="OrthoDB" id="665824at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PTHR43382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="SSF64586"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000249152" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <sequence length="488" mass="56295" checksum="0B2670FE7DAD5123" modified="2004-10-11" version="1">MKEEKSFVKEITPQSEDFSRWYIDVIRKADLMDYTPVRGCIVFKPDGYELWERIQAGLDKRFKETGHRNAYFPMLIPESFFQKEKEHVEGFNPELPWVTEAGGEKLEERLALRPTSETIIGHMYAQWIQSYRDLPVLINQWANVFRWEKRTLPFLRTSEFLWQEGHTAHATEEEAREETMRMLEVYRDFVETEMAIPLYVGQKTPSEKFAGAVDTYSIEAMMKDGKALQAGTSHYLGQNFAKGFEIKFLDRDNQLKYVHTTSWGVSTRLIGALIMVHGDDRGLALPPRLAPIQVIMIPVGPPKFRDKVMARFDPLFDALKAAGVRVKADLREETPGWKFNEWEMRGVPLRIEIGPRDVDNKQCLMVRRDTGEKIPVPLDEAVERVQALLEEIQRNMFVKAKEFRDAHSHLHINTLAELKAHIARCEETGEIAGFVLAGWCGDAACEAKVKEETKFTSRNIPFHPPARKQVCLCCGKEAQHTVWFARAY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-11-08" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC6</accession>
    <name>TRMFO_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO</fullName>
            <ecNumber evidence="1">2.1.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Folate-dependent tRNA (uracil-5-)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Folate-dependent tRNA(M-5-U54)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmFO</name>
        <name type="synonym">gid</name>
        <name type="ordered locus">STH1482</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:16873"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH + uridine(54) in tRNA = (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine(54) in tRNA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:62372"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.74"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family. TrmFO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.74" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195612.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PC6"/>
    <dbReference type="SMR" id="Q67PC6"/>
    <dbReference type="STRING" id="292459.STH1482"/>
    <dbReference type="PRIDE" id="Q67PC6"/>
    <dbReference type="EnsemblBacteria" id="BAD40467">
        <property type="protein sequence ID" value="BAD40467"/>
        <property type="gene ID" value="STH1482"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1482"/>
    <dbReference type="eggNOG" id="COG1206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033057_1_0_9"/>
    <dbReference type="OMA" id="MHRNTFL"/>
    <dbReference type="OrthoDB" id="516534at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030698">
        <property type="term" value="F:5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047151">
        <property type="term" value="F:methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01037">
        <property type="entry name" value="TrmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004417">
        <property type="entry name" value="TrmFO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF2">
        <property type="entry name" value="PTHR11806:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00137">
        <property type="entry name" value="gid_trmFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000117280" description="Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01037"/>
        </source>
    </evidence>
    <sequence length="447" mass="48790" checksum="4179462DA339AC52" modified="2004-10-11" version="1">MTEPHVTVIGAGLAGSEAAWQAARLGVKVTLYEMRPHVTTAVHRTGLFAELVCSNSLRGAGLENAVGLLKEEMRRLGSLILREALQHAVPAGGALAVSREEFAAGVTAALTSHPNITVVREEVREIPPDGVVVIASGPLTSAPLAEAIRRFTGEESLAFYDAAAPIVNIETVNMDKVFRMSRYGKGEGDDYLNCPLTREEYEAFYEALVTAEKAMPHNPEDAQVCFFEGCLPVEEIARRGPDALRYGPMKPVGLIDPRTGRRPWAVVQLRQDNAAGTLYNMVGFQTSLKWSEQKRVFRMIPGLEEAEFERYGVIHRNTFMKSPRLLYPTGESRQRAGLFFAGQMTGVEGYVESAAGGLVAGINAARRALGLEPVTFPRETAIGSLLHYITHADPEHFQPMNIAFGLMPPLEGPKIRDKRARKRAISERALDVLAAWAPGHLGAPLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC7</accession>
    <name>Q67PC7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">topA</name>
        <name evidence="13" type="ordered locus">STH1481</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1481"/>
    <dbReference type="EnsemblBacteria" id="BAD40466">
        <property type="protein sequence ID" value="BAD40466"/>
        <property type="gene ID" value="STH1481"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1481"/>
    <dbReference type="eggNOG" id="COG0550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_4_3_9"/>
    <dbReference type="OMA" id="PECKYTR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="12">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="163"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="359"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="735" mass="83210" checksum="2C0A3C19F04B345F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE5</accession>
    <name>Q67PE5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MazG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195593.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1463"/>
    <dbReference type="EnsemblBacteria" id="BAD40448">
        <property type="protein sequence ID" value="BAD40448"/>
        <property type="gene ID" value="STH1463"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1463"/>
    <dbReference type="eggNOG" id="COG1694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142229_1_0_9"/>
    <dbReference type="OMA" id="MADVLWV"/>
    <dbReference type="OrthoDB" id="1760741at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11531">
        <property type="entry name" value="NTP-PPase_BsYpjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012359">
        <property type="entry name" value="MazG-related_YpjD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42692:SF1">
        <property type="entry name" value="PTHR42692:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029904">
        <property type="entry name" value="UCP029904_pph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="12206" checksum="F1C0F9A5C8E96301" modified="2004-10-11" version="1">MDLRDVQREVDRWVAPFGYWDPLANLVRLTEEVGELAREINHRWGPKKKKPTEDTGDVALELGDILFVVAVLANQLNIDLEEAFRRVMDKYRTRDADRWRPKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG8</accession>
    <name>Q67PG8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195570.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1440"/>
    <dbReference type="EnsemblBacteria" id="BAD40425">
        <property type="protein sequence ID" value="BAD40425"/>
        <property type="gene ID" value="STH1440"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1440"/>
    <dbReference type="eggNOG" id="COG1193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078484_3_2_9"/>
    <dbReference type="OMA" id="GAREYAD"/>
    <dbReference type="OrthoDB" id="1602369at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="155" mass="17575" checksum="B0E7885390FDD1BA" modified="2004-10-11" version="1">MEIMALIDRLEELIQQATRVPLTGKILLDPDEILAIVDEMREVVPSEIREANRVARDRETILAEAREQAEEILREARALAAQLTSEAAVTKEAQTQADELIDQAKRVAREIRQNALEWADELFARAQPELERIAADTQRAVAAVRKAREELQNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH4</accession>
    <name>Q67PH4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1434</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1434"/>
    <dbReference type="REBASE" id="10268">
        <property type="entry name" value="M1.StpORF1434P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40419">
        <property type="protein sequence ID" value="BAD40419"/>
        <property type="gene ID" value="STH1434"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1434"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060286_1_0_9"/>
    <dbReference type="OMA" id="VFIGDMY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0680">Restriction system</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="6">
        <location>
            <begin position="55"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="33610" checksum="7CD21F069A982F8C" modified="2004-10-11" version="1">MATTSSGQKGLKPHHIDMGERGIYHTDNRLNDLTGKEWVFATRSVINKSYPPSFQFALRSRHGGQKPPELCADLIRTFTKSGERVLDPFMGVGGTLIGATISGRRAVGVEINPRWIAIYREVCSLEGLPEQEAVCGDSRTVLAGFEPESFDLVLTDVPYWNMDRRRRSKGKFKRADGPAVEPRRSKLSPFAPDETETDVTGMQGKEEWLDTMRAVFAAALRLLRPRRYMAVFIGDMYHSGRYHMLSAELAGLLESLGLVLKANLIWYDVSKKLHVYGYRYEFIPSMVHQNILVLRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK8</accession>
    <name>Q67PK8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional ligase/repressor BirA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Biotin--[acetyl-CoA-carboxylase] ligase</fullName>
            <ecNumber evidence="2">6.3.4.15</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Biotin--protein ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Biotin-[acetyl-CoA carboxylase] synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">birA</name>
        <name evidence="4" type="ordered locus">STH1400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:11756"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.15" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195530.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1400"/>
    <dbReference type="EnsemblBacteria" id="BAD40385">
        <property type="protein sequence ID" value="BAD40385"/>
        <property type="gene ID" value="STH1400"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1400"/>
    <dbReference type="eggNOG" id="COG0340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_0_0_9"/>
    <dbReference type="OMA" id="AVWKHIE"/>
    <dbReference type="OrthoDB" id="1842956at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00978">
        <property type="entry name" value="Bifunct_BirA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030855">
        <property type="entry name" value="Bifunct_BirA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0092">Biotin</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="3">
        <location>
            <begin position="71"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Biotin binding" evidence="2">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="35980" checksum="26E88765F8FB3A60" modified="2004-10-11" version="1">MTSRTAPELLSLLTAAGGGWVSGAEISRRLGVSRTAIWKHIEALRLLGYEIEAAPRKGYRLVARPDLLTPEEVRAGLATRRLGRVVEYRETVGSTNDLAKEMARQGAPEGLLVLAEQQTAGKGRLGRSWTTPPGAAIAMSLVLRPDLPPHAAPRITLAAAVAVTEAVREAAGVAAGIKWPNDVQVNGRKLCGILTEMEAEMDRVAFVVLGIGLNVTLRREQMDPSFRETATSLALEGAPPVRRAALVQAILARFEAVYDELLGDRFDRVLDRWRALSVTLGQAVRALGLDGQPVLEGVAEEVDQEGALLVRDGAGQVHRVVSGEVSLRPAHAQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX0</accession>
    <name>Q67PX0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195419.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40273">
        <property type="protein sequence ID" value="BAD40273"/>
        <property type="gene ID" value="STH1288"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1288"/>
    <dbReference type="eggNOG" id="ENOG5033IW8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1874384_0_0_9"/>
    <dbReference type="OMA" id="YAIFACE"/>
    <dbReference type="OrthoDB" id="1984331at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="15311" checksum="68FE54AA30B128D4" modified="2004-10-11" version="1">MQHIRNPRCFLVYALAPEGGSPAEANRLLNAYVADERRGLAVFHDHFIGRPGGVAIFFAETAEQRAAIADLGPLEGWHVEIRPLIYSRSPAGFDEQTAYTLRAYRNADWEKLRVEQRPRYGDPAREAETAEEDVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ5</accession>
    <name>Q67PZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195394.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1263"/>
    <dbReference type="EnsemblBacteria" id="BAD40248">
        <property type="protein sequence ID" value="BAD40248"/>
        <property type="gene ID" value="STH1263"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1263"/>
    <dbReference type="eggNOG" id="COG2871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2496747_0_0_9"/>
    <dbReference type="OrthoDB" id="1837979at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="86" mass="10078" checksum="8B3EF92AD1422B41" modified="2004-10-11" version="1">MRRIKYRDEEYLVHTRSGMRLIDALVEQGVPVTCDCDGRAHTTEQCLVQWPKDTAFLLTEPTEFERRQLGDRLEKGHRLACQALFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q30</accession>
    <name>SEPF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sepF</name>
        <name type="ordered locus">STH1228</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Localizes to the division site, in a FtsZ-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SepF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195359.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q30"/>
    <dbReference type="SMR" id="Q67Q30"/>
    <dbReference type="STRING" id="292459.STH1228"/>
    <dbReference type="EnsemblBacteria" id="BAD40213">
        <property type="protein sequence ID" value="BAD40213"/>
        <property type="gene ID" value="STH1228"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1228"/>
    <dbReference type="eggNOG" id="COG1799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1348356_0_0_9"/>
    <dbReference type="OMA" id="EMHRIAN"/>
    <dbReference type="OrthoDB" id="2064885at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01197">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023052">
        <property type="entry name" value="Cell_div_SepF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35798">
        <property type="entry name" value="PTHR35798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000334121" description="Cell division protein SepF">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="167"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="203" mass="22169" checksum="9A664980ED80FE4A" modified="2004-10-11" version="1">MPNKPGLWSRLVDYLGFGPEEDEFEDGELEQVQPAYQEEPPRRSAPERRGQVVPISAVPSKQGTVKVVVVEPRSFEEVQTIVDQMKARRPVILNLESLDKDLAQKILNFLNGAIYALNGETQRVSAGIFFYAPPGIDVSTMGRGLTGTAIGGGAVDLPPGVLEKLMGTASGSQEGDLLARTARRSEEGDRTGADRSKFDWRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ9</accession>
    <name>Q67QJ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195191.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1059"/>
    <dbReference type="EnsemblBacteria" id="BAD40044">
        <property type="protein sequence ID" value="BAD40044"/>
        <property type="gene ID" value="STH1059"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1059"/>
    <dbReference type="HOGENOM" id="CLU_1389611_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="196" mass="20911" checksum="62499E581D69CAA8" modified="2004-10-11" version="1">MSTPDTAPQPENHAQRHGLAVSLLRGLGLVVLMAAISAGTAFLIVELRMNAIAAATAAAEDDLTALRQATLDEVAALRAELASTTAELQAEVRRQREELEAEMKRVQEAAVAAGVLLEQEGELTGLEARLKEIETLKLDLKSAREEMDAKLQALEASLKDQLASSEQETAAALSLEMSVKGLLIKAQGEVLLAQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR1</accession>
    <name>Q67QR1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum transport system permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH997</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the binding-protein-dependent transport system for molybdenum; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195129.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH997"/>
    <dbReference type="EnsemblBacteria" id="BAD39982">
        <property type="protein sequence ID" value="BAD39982"/>
        <property type="gene ID" value="STH997"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH997"/>
    <dbReference type="eggNOG" id="COG4149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_3_9"/>
    <dbReference type="OMA" id="MYSFIET"/>
    <dbReference type="OrthoDB" id="1915735at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011867">
        <property type="entry name" value="ModB_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02141">
        <property type="entry name" value="modB_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="12"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="23382" checksum="E5CA20646E3777D7" modified="2004-10-11" version="1">MSDLGAIDWFPVVLSLRVAFLATLSAALAGVPLAYGLARRRLPAPRLLSALTTLPLVLPPTVVGYYLLLLIGRQGPLGRLLEGMGIRLVFTWHAAVLAAAVVSFPLVVRSAQAAFESMDPALEAAARTLGRSELSIFFTVTAPLAWRGILAGAVLAFARAMGEFGATLMVAGNIPGRTQTLSVAIYDAVQSGQYGLANALVLLISLVSVGVLVVLTGFGEGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW7</accession>
    <name>Q67QW7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH941"/>
    <dbReference type="EnsemblBacteria" id="BAD39926">
        <property type="protein sequence ID" value="BAD39926"/>
        <property type="gene ID" value="STH941"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH941"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1019144_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_12" evidence="1">
        <location>
            <begin position="31"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="29433" checksum="92D4E459B2D6F66E" modified="2004-10-11" version="1">MHGIAAQMALYRESARRLCELAGLRPGMAVVDLGAGTGLAGLAALELVPEGLELVVVEPNPSLLAAARARIGDRAGAYHTVDPVAAADLVPGKVDRVICNLALLSFRDPEGVLRAWRNRIKPTGRFCFSLSGTYFNVLRDQVTPQYVFIQELHRQGLLPRGLPSVERLPNQRSVEGTLKGAGYKPARYEVQEIPTARPETEPGGELYNLMRIHPALPGKDHRDAVERTLAALPAAAPAIASRGARWQVVHFVAQPALTPEELLLERFGGGPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY1</accession>
    <name>Q67QY1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH927"/>
    <dbReference type="EnsemblBacteria" id="BAD39912">
        <property type="protein sequence ID" value="BAD39912"/>
        <property type="gene ID" value="STH927"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH927"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_8_1_9"/>
    <dbReference type="OMA" id="EYVPAYY"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="16229" checksum="AEB65560D3916335" modified="2004-10-11" version="1">MRLGSPMPPLEGATGSYNAEGFDPAQLSGHPVLIHFWAVSCHICHEVMDQVLQYRDMYQPKGLKVVAIHMPKEEGDLDTAKVERDIAQYGITQPVLLDHRHALAQAFQNQFVPAFFLFDAQGNLFFRAAGDKGLQNLGPKLEQLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R29</accession>
    <name>Q67R29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein, proline-rich</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195011.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH879"/>
    <dbReference type="EnsemblBacteria" id="BAD39864">
        <property type="protein sequence ID" value="BAD39864"/>
        <property type="gene ID" value="STH879"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH879"/>
    <dbReference type="eggNOG" id="COG1559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1209288_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="24140" checksum="591BBB99FDD7A8F9" modified="2004-10-11" version="1">MTWILLAAGLLLVVLGVWGGPATRRRPEKAERIPREATVQEAWDILTSPVEYDEELIKIPDHQPWFLPGGDRRFQRGLAVGLGAGLMLAVLLAPLIARPKAPASPEPGDNTGVVQGPGQQDPGGWAPDPSFPPNPPPAADPEPDPEPEPDPGNAGEPEPAPSQTVTVTVAMGSSSQDIAALLKEAGLVKSEEDFLSVVAELGVETRLKAGTFDIPTDASPIEIVNLLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R46</accession>
    <name>Q67R46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH862"/>
    <dbReference type="EnsemblBacteria" id="BAD39847">
        <property type="protein sequence ID" value="BAD39847"/>
        <property type="gene ID" value="STH862"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH862"/>
    <dbReference type="HOGENOM" id="CLU_1365623_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="21300" checksum="30C05CA2EC92AEC4" modified="2004-10-11" version="1">MAVAAVPGHRRRVPFECEVWRGRGLRYRRGVAVAAPKRHEVSYGAHDQAERRRSRSSLTWPSVPTRKTPAPSRFVLQRASDVPQQVSCTVRAGALARFQVSVGAGAGKRSGPSPSSVTAGVGLAPATSSRNAAQVDMGVPRTAELSNPRASFDSKRSPNVLVPHMRTGNRMGGRLARSNCAQASPGDSKSRIWPIPTGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R96</accession>
    <name>Q67R96_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39797">
        <property type="protein sequence ID" value="BAD39797"/>
        <property type="gene ID" value="STH812"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH812"/>
    <dbReference type="HOGENOM" id="CLU_1460592_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="185" mass="21360" checksum="01577C19C3C16167" modified="2004-10-11" version="1">MQSPEGLHKSGENFVLATKRYLEATLVIDVLWTVYDGNGMTVLRGLDGQPHTFDALARLKEGEKRPIYIEAKKRTSANINAEYDDFIAKSFSCYMAERWRPDWNPYFMFVTDHPFKMKDYARLLEADYLLTFLGTFEKYGIANAALEHVVDFKDRVWLVIWSNRQELMCVTNPALYQKLVVDGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC9</accession>
    <name>Q67RC9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipopolysaccharide N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194912.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH779"/>
    <dbReference type="EnsemblBacteria" id="BAD39764">
        <property type="protein sequence ID" value="BAD39764"/>
        <property type="gene ID" value="STH779"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH779"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_38_2_9"/>
    <dbReference type="OMA" id="VHNEMFA"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="21"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="188"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="381" mass="42623" checksum="70BB5461E99995B2" modified="2004-10-11" version="1">MRSLMICTEKLPVPPVRGGAIQTYIHAVAPRLGRHHRVTVLGVADPELPDREIRDGVEYWRVPAGTFPQYRDRVVNLLRRAGRKFDVVHLFNRPRLVLPVREALPESRLILSLHNEMFRPEKIGPEEARAAIAATERIVTVSNFIGRGVEALYPEARGKWSTIYSGVDLDRFLPIWTPEARRIRQRLRAEHGLGRGPILLYVGRLSPKKGADVVARALGPVARRYPEATLVLVGSKWYGEDRISDYVAYVRALAARAPMKVLATGYVPADRVHEWFAAGDLFICASQWEEPLARVHYEAMAAGLPIITTARGGNPEVVRGQGNGLVVEEAGSPEIFARAILRLLGDADLRTRMSRRGRALAEAHFGWDRVAAEIRALWERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD3</accession>
    <name>Q67RD3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194908.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH775"/>
    <dbReference type="EnsemblBacteria" id="BAD39760">
        <property type="protein sequence ID" value="BAD39760"/>
        <property type="gene ID" value="STH775"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH775"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_10_0_9"/>
    <dbReference type="OMA" id="RMRAFNG"/>
    <dbReference type="OrthoDB" id="415910at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="2">
        <location>
            <begin position="12"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="1">
        <location>
            <begin position="139"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="393" mass="43037" checksum="FA1D59C263328D93" modified="2004-10-11" version="1">MLLTPRHELDLRIGRLQAALRDLGLDGALLHSTTSLLYFTGSAQQGHLWVPANGEPRYLVRRVLERARRECALPGIEPLTTLRALPDHLGGARRIGMELDRLPVAQFEVYRRHLPGVDAADVGPVLRRLRSVKSPWEVERIRAAARAADETYRALFAALREGMTELELSVVGEGAQRLAGAQGMIRWHAHNAFESPAMMVLAGETALAFSFADTPFGGEGLTPAAPYGASRRPIRRDEPVCVDFPTVVDGYVHDQTRTMVIGKLPRQLVEAHDTARQILDMVAREARPGATGQQLWERSVDIARRAGLEEHFMGAGNSRVRFVGHGVGLELDEWPILAPKQTTPLEAGNVIAVEPKFFFPGVGAVGLESTFLVTPDGAERLSITPEELGVKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG1</accession>
    <name>Q67RG1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH747</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194880.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH747"/>
    <dbReference type="EnsemblBacteria" id="BAD39732">
        <property type="protein sequence ID" value="BAD39732"/>
        <property type="gene ID" value="STH747"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH747"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_2_9"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="1763210at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="63"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="283" mass="30313" checksum="20FF988DB9457547" modified="2004-10-11" version="1">MTRQQIADRVATAVLWAAGILILLILAGFLLPILIEGLPVVDWQFLTTRSSAYRAGGGVGAQLFNTIYITGLSLLFSLPVGVGAGIYMAEYAKPGRMTDLIRLSVEALASVPSIVLGLFGMILFVNYMGWGFTILGGSLSLALLNLPVLVRNTEEAVRAVPQSYREGSLGLGATQWQTISRVVLPSALPGILTGLTLVAGRALGETAVLIYTAGVTASRHFPNPDLMIAGETLAVRIWSVTSEGLVPDADRIAMGASALLILLVLFVNFLIALPLYRYRRRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH1</accession>
    <name>Q67RH1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oligoendopeptidase F</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH737</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Has oligopeptidase activity and degrades a variety of small bioactive peptides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194870.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH737"/>
    <dbReference type="EnsemblBacteria" id="BAD39722">
        <property type="protein sequence ID" value="BAD39722"/>
        <property type="gene ID" value="STH737"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH737"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_2_0_9"/>
    <dbReference type="OMA" id="YRQTMFA"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004438">
        <property type="entry name" value="Peptidase_M3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00181">
        <property type="entry name" value="pepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="8">
        <location>
            <begin position="113"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="7">
        <location>
            <begin position="204"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="599" mass="68484" checksum="FE9A2661E27A1A8E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH2</accession>
    <name>Q67RH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH736</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194869.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH736"/>
    <dbReference type="EnsemblBacteria" id="BAD39721">
        <property type="protein sequence ID" value="BAD39721"/>
        <property type="gene ID" value="STH736"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH736"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049710_2_0_9"/>
    <dbReference type="OMA" id="SAWLCVI"/>
    <dbReference type="OrthoDB" id="955123at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017562">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03144">
        <property type="entry name" value="cytochr_II_ccsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="7">
        <location>
            <begin position="75"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="399" mass="43958" checksum="FF6563ACD20857F7" modified="2004-10-11" version="1">MIQVSSYLFLGAFAAYVASAILYVTRLTLRRRDPRYFDLADRLGRHGYHAAVAGVILQGLAIVARWMGSGQVPLANMFEYMSFLGWSIMLFFVILSAWYKLPGLGAFVAPVGVVVIAYASVFPTEVRPLAPVLQSVWLPLHVSVAALGEGAFAVSFGAALMYLLRVRKEQSSRWEEMGLEFFFFCGAMLTAFIVLALGFKLSGYRAAVTNLAGGTTVLEYTLPPFIGPLGSTRGEASFLGLQLPLVHAPTWMRGAYAARNLNTLVLSTLFGVILYGILRLIARGPLRELGTRAVGNMNPKLLDEVSYRGIAVGFPLFTLGGLVFAMIWAQKAWGRYWAWDPKETWALITWLFYSGYLHMRIVRGWEGRAAAWVSALGFIIVLFTLVGVNLLVSGLHSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH5</accession>
    <name>Q67RH5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194866.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH733"/>
    <dbReference type="EnsemblBacteria" id="BAD39718">
        <property type="protein sequence ID" value="BAD39718"/>
        <property type="gene ID" value="STH733"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH733"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1089591_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="27266" checksum="8647A8221B3E7CD5" modified="2004-10-11" version="1">MPTCPSCGHEATGPYCDVCGGPVAPHRDRPRRGAKAPAPAPARGGHSPAQGRSPRRHVPTVALALCGLLLFGVGFLSGVWLGRGSAVTGTALYPTPGIDQAALETLTPLAQANFFLETGVALLNQGHRSAAVSEFRKAITAFETVLRSEPDNLFAGTYLGLTCYYAGDRQKAEEALQAVLERDPGYLWAIFNLAWIYEVEGETDAAVAMYQRYLNAAPQERENTLKYAEQPDLIDQQINAAREAVARLSGGGNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ3</accession>
    <name>Q67RJ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194848.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39700">
        <property type="protein sequence ID" value="BAD39700"/>
        <property type="gene ID" value="STH715"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH715"/>
    <dbReference type="HOGENOM" id="CLU_2884306_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="63" mass="7047" checksum="4D5A045F13C4E8AC" modified="2004-10-11" version="1">MDSFQLVFLFVAFLVLVFTVLKWVRLLRKLDAALQAAQEAARRQRGQDAGLQDAGSSSPVIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK4</accession>
    <name>Q67RK4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH704"/>
    <dbReference type="EnsemblBacteria" id="BAD39689">
        <property type="protein sequence ID" value="BAD39689"/>
        <property type="gene ID" value="STH704"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH704"/>
    <dbReference type="eggNOG" id="COG1022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_5_9"/>
    <dbReference type="OMA" id="GEGEKMP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="18"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="659" mass="73885" checksum="0DCD538AE56C225D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP9</accession>
    <name>Q67RP9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase III subunit alpha</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnaE1</name>
        <name evidence="14" type="ordered locus">STH659</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-C family. DnaE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH659"/>
    <dbReference type="EnsemblBacteria" id="BAD39644">
        <property type="protein sequence ID" value="BAD39644"/>
        <property type="gene ID" value="STH659"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH659"/>
    <dbReference type="eggNOG" id="COG0587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001600_0_0_9"/>
    <dbReference type="OMA" id="KQRFHLC"/>
    <dbReference type="OrthoDB" id="561611at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041931">
        <property type="entry name" value="DNA_pol3_alpha_thumb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00594">
        <property type="entry name" value="polc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="13">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1080"/>
            <end position="1178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD39644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1252" mass="138788" checksum="C777AD07583D79BD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S19</accession>
    <name>Q67S19_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Magnesium transporter MgtE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH539"/>
    <dbReference type="EnsemblBacteria" id="BAD39524">
        <property type="protein sequence ID" value="BAD39524"/>
        <property type="gene ID" value="STH539"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH539"/>
    <dbReference type="eggNOG" id="COG2239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037408_1_1_9"/>
    <dbReference type="OMA" id="TMTVAVR"/>
    <dbReference type="OrthoDB" id="1239195at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006669">
        <property type="entry name" value="MgtE_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43773">
        <property type="entry name" value="PTHR43773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00924">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="SSF161093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00400">
        <property type="entry name" value="mgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="138"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="8">
        <location>
            <begin position="202"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="453" mass="48797" checksum="7B42D50BC86C7487" modified="2004-10-11" version="1">MNQELIGTLDQTIVLLQQHRLDELRELLSELHPADLAEVLQELEEEDRTAVVQLLAGPTAAEAISELEPEEQAELVTALPPAQAKAILDEMSADDAADLFAELDPEKAGALLDLMAQEEATDVRELLGFREDSAGGIMTTEFVAVQAHRTAAEAIEQLRREAPSAETAYYVYVIDAQERLVGVLSLRELIVAPPETEVRAIMRTNPVSVHVNEDQEEVARVVQKYNLLAVPVVDDQGILRGIVTVDDVIDVLEEEATEDIYRAGGVSEAEYETDLEGAIWPSVRARLPWLLGLLFLSLLSGKVIETFTPLINQVSALAVFITTMAGGSGNAATQALTVMVRGLATGEVERGQLIRAVWREARIGLVTGLICGLVLGGAAMLWNGSPWVGLIVGLALTINLMLAKAAGSLVPVIIQRLGLDPAVASGPFIATITDTFSMLVYFTIASLVLHAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S68</accession>
    <name>Q67S68_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194624.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH490"/>
    <dbReference type="EnsemblBacteria" id="BAD39475">
        <property type="protein sequence ID" value="BAD39475"/>
        <property type="gene ID" value="STH490"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH490"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_4_9"/>
    <dbReference type="OMA" id="FSEEPYG"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269655" evidence="4">
        <location>
            <begin position="28"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="52"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="29868" checksum="9F4C370076F06DD4" modified="2004-10-11" version="1">MKRRWGATVAALLCAAVLAGCGGRSSAPAGGGAGGGASDLPAQVQKIRDAGVLRVGVKEDVPKFGYFDPAKNAHEGLEIDLARRIAKEIFGDESKVQFTGVNAKTRGPALDNGEIDLVIATFTITEERKKSWNFSDPYFTDYVGFLVLADSGIQSWKDLDGKVIGVAQSASTRAALTEQAEKDGIKVEFMEFATYPEIKAALDSRRIDAFSVDQSILWGYKDDKNVMLPDKFAPQEYGIATKLSNTELAEFVNNILKGMKESGELDQLYEKWGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S70</accession>
    <name>Q67S70_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH488</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH488"/>
    <dbReference type="EnsemblBacteria" id="BAD39473">
        <property type="protein sequence ID" value="BAD39473"/>
        <property type="gene ID" value="STH488"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH488"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_0_9"/>
    <dbReference type="OMA" id="WQSMKLV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="21"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="219" mass="24754" checksum="359A5F7F38F16EDB" modified="2004-10-11" version="1">MIEELAVLAEWHNVRFMLDGLLITLRISALTIVFSFFLGTLLGVMRYSGHWLFARIAAVYIESFRASPLILLILLFRFTMPMKPVDSGTLAMTLFTAAIVGEIVRGGLNSVDKGQWEAARSQGFTWWQTMQHIVLPQALRKMIPPLTGQFITVVKDTSYVWVTGVQELTGKGVIILGKHGSTVQFFAIFGTIGLIYYVLCLTINRIGLWQERRMSWLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S87</accession>
    <name>Q67S87_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="8">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">potA</name>
        <name evidence="10" type="ordered locus">STH471</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194605.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH471"/>
    <dbReference type="EnsemblBacteria" id="BAD39456">
        <property type="protein sequence ID" value="BAD39456"/>
        <property type="gene ID" value="STH471"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH471"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="IHVMRFN"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015594">
        <property type="term" value="F:ABC-type putrescine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03300">
        <property type="entry name" value="ABC_PotA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017879">
        <property type="entry name" value="PotA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="348" mass="38480" checksum="3652E5D14383F0A4" modified="2004-10-11" version="1">MVEFRGVSKRFDSTEALNDLTLEIRPGEFLTLLGPSGCGKTTTLRLLAGFEEPTSGEIWLAGRPVQGLPAYKRPVNTVFQHYALFPHMTVFDNVAFGLRMKKVAPAEIRRRVAEALELVRLGDMGSRRPHELSGGQQQRVALARALVMEPKVLLLDEPLGALDLQLRRAMQVELRQLQRKLGATFLYVTHDQEEALAMSDRIAVMRNGRVEQVGTPEEVYLRPATRFVAQFLGEANLLEGEGAANGRLRWGDYHLQVSRHLSPGKTTVALRPEQVQVRWPGSTEPARPAENRVHGIVAERLFVGTGTRLMVEVAGRRLAALLPAGPGPDIGDQVELRFAAEHCVVVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S90</accession>
    <name>Q67S90_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spermidine/putrescine ABC transporter spermidine/putrescine-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH468"/>
    <dbReference type="EnsemblBacteria" id="BAD39453">
        <property type="protein sequence ID" value="BAD39453"/>
        <property type="gene ID" value="STH468"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH468"/>
    <dbReference type="eggNOG" id="COG0687">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_1_3_9"/>
    <dbReference type="OMA" id="SNWTEYM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019808">
        <property type="term" value="F:polyamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015846">
        <property type="term" value="P:polyamine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001188">
        <property type="entry name" value="Sperm_putr-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019574">
        <property type="entry name" value="Periplasmic_polyamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00909">
        <property type="entry name" value="SPERMDNBNDNG"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Spermidine-binding" evidence="1">
        <location>
            <begin position="174"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR019574-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="353" mass="39410" checksum="54B7B1E20F6C93E4" modified="2004-10-11" version="1">MALSGCSVGGSTGAASDLPRPERDPSVDMSRLSAELNVFTWTEYLPQSIIREFERVYNVRVNYDTYSSNEEMHAKLKAGASGYDIIIPEIYMIKVLRREGLLEELDHANIPNLKNIDDRFRGLDHDPELRYSVPYVWGTTGIVINTEKISPDQMTSWADLWNPEFQGRLVVPDDAREMIGIALRTEGYGVNTTDPDALARAKEKLKALKPNVKAFNSDSPKDLLLGGEVWGGVVWSGEAALVMRESDAFQFILPPEGVTMWVDNVAIPKDAPHKYTAEVFINFLLDPVVSARISAEYPYGNPNKAAMPYISAEDLANPAINPPAEWLRNAESLDDLGDQMNQIYDQIWTEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB2</accession>
    <name>Q67SB2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH446"/>
    <dbReference type="EnsemblBacteria" id="BAD39431">
        <property type="protein sequence ID" value="BAD39431"/>
        <property type="gene ID" value="STH446"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH446"/>
    <dbReference type="eggNOG" id="COG2931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_485638_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="561" mass="58515" checksum="8342A5EB7367BC0B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI0</accession>
    <name>Q67SI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DinB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194512.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH378"/>
    <dbReference type="EnsemblBacteria" id="BAD39363">
        <property type="protein sequence ID" value="BAD39363"/>
        <property type="gene ID" value="STH378"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH378"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116635_0_0_9"/>
    <dbReference type="OrthoDB" id="2047691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007837">
        <property type="entry name" value="DinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05163">
        <property type="entry name" value="DinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="16844" checksum="47D57E47E4C4C821" modified="2004-10-11" version="1">MLEGNRRLTLRVVELFPEDALFHFTPAPPLRPFADMVNEILEMEEGYVRGIATREWTFSPKRVVKTKQELLEACAAVRRRTLELWPRITAARLTEEEIDPFFGSEPQSNLSRLVYTLENEIHHRGQGYTYLRILGIQPPAFYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ4</accession>
    <name>Q67SJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Permeases of drug/metabolite transporter superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH364"/>
    <dbReference type="EnsemblBacteria" id="BAD39349">
        <property type="protein sequence ID" value="BAD39349"/>
        <property type="gene ID" value="STH364"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH364"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_21_3_9"/>
    <dbReference type="OMA" id="TFLAVQY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="9"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="150"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="31806" checksum="348B8A2A289F6AC2" modified="2004-10-11" version="1">MQIRTWQADLVLLGITAIWGATFPMVKNATSLEAGGVPTYWFLAVRFLMAALLLAAVFHRRLRRLPAATWRAGVLLGIFLFAGYAFQTFGLAYTTSAKAAFITGLSVVIVPVLSLVWLRTAPSPAAWLGVLTALAGLAMLSLDGSLAPSRGDLLVLMGAFGFGLHVAGVSRFGAAHDRYALAVIQLGTAGVLSALLHLADRGTVLPGVAGVLWWGGPAHVGTAVIVCGLLATAAAYLLQNLLQPYTTATHTALIFAAEPVWGAVFAYLLAGEVLTPRGYAGAALIIAGMLMAELPAGRDRRAAAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL5</accession>
    <name>RNPH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease PH</fullName>
            <shortName evidence="1">RNase PH</shortName>
            <ecNumber evidence="1">2.7.7.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rph</name>
        <name type="ordered locus">STH343</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + tRNA(n+1) = a ribonucleoside 5'-diphosphate + tRNA(n)</text>
            <dbReference type="Rhea" id="RHEA:10628"/>
            <dbReference type="Rhea" id="RHEA-COMP:17343"/>
            <dbReference type="Rhea" id="RHEA-COMP:17344"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:173114"/>
            <dbReference type="EC" id="2.7.7.56"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexameric ring arranged as a trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.56" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194477.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SL5"/>
    <dbReference type="SMR" id="Q67SL5"/>
    <dbReference type="STRING" id="292459.STH343"/>
    <dbReference type="EnsemblBacteria" id="BAD39328">
        <property type="protein sequence ID" value="BAD39328"/>
        <property type="gene ID" value="STH343"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH343"/>
    <dbReference type="eggNOG" id="COG0689">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050858_0_0_9"/>
    <dbReference type="OMA" id="KGKGQGW"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009022">
        <property type="term" value="F:tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11362">
        <property type="entry name" value="RNase_PH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00564">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002381">
        <property type="entry name" value="RNase_PH_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018336">
        <property type="entry name" value="RNase_PH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01966">
        <property type="entry name" value="RNasePH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01277">
        <property type="entry name" value="RIBONUCLEASE_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000139940" description="Ribonuclease PH">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <note>substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <note>substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="273" mass="28989" checksum="9E19D363135329F5" modified="2004-10-11" version="1">MRQDGRLPHEMRPVRITRHYNIHAEGSVLIEVGRTRVICTATLEDRVPPFLRGRGEGWITAEYGMLPRATGQRTAREAARGRQGGRTMEIQRLIGRALRSVIDLAALGERTLIIDCDVIQADGGTRTASITGAYVAMVDALAGLRAAGLIDRLPVKDYLAATSVGVVGGVPVLDLTYEEDSRAAVDLNLVMTGSGEVVEIQGTGEERPFTRRELEELLALAESGVRRLVALQREQLGPLGVEVGAVVTEASGIGHQEPGEGAGVSLAPGGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW8</accession>
    <name>Q67SW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194375.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH240"/>
    <dbReference type="EnsemblBacteria" id="BAD39225">
        <property type="protein sequence ID" value="BAD39225"/>
        <property type="gene ID" value="STH240"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH240"/>
    <dbReference type="eggNOG" id="COG3428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129146_1_0_9"/>
    <dbReference type="OMA" id="LMNINID"/>
    <dbReference type="OrthoDB" id="1973893at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030153">
        <property type="term" value="P:bacteriocin immunity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009589">
        <property type="entry name" value="SunI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06713">
        <property type="entry name" value="bPH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="16168" checksum="41BEA4C7F606D406" modified="2004-10-11" version="1">MRYFPSERSLWFGLIVWVVVPGVVAFLLVLLLREPTRWWDWILAGAAPAALEGLLIWIWFGTGYAVTENELVIRSAFLTWRIPLAAIRRVRPTRSPLTSPALSMDRLEVRTNKGSAPLISPRNRSEFLALLRERCPQADIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T40</accession>
    <name>Q67T40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched-chain amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH168"/>
    <dbReference type="EnsemblBacteria" id="BAD39153">
        <property type="protein sequence ID" value="BAD39153"/>
        <property type="gene ID" value="STH168"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH168"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_447534_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="265"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="610" mass="64122" checksum="0081309C0C451BFD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T52</accession>
    <name>Q67T52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH156</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194291.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH156"/>
    <dbReference type="EnsemblBacteria" id="BAD39141">
        <property type="protein sequence ID" value="BAD39141"/>
        <property type="gene ID" value="STH156"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH156"/>
    <dbReference type="eggNOG" id="COG0351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_3_9"/>
    <dbReference type="OMA" id="DNRHTHG"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="3">
        <location>
            <begin position="13"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="28998" checksum="81CBE739B93799F4" modified="2004-10-11" version="1">MTLPRALTIAGSDSGGGAGIQADLKTFTALGVCGTSAITCVTAQNTLGVTGVYPLTAEQVAAQLDAVLADIGTDSAKTGMLYDPQIIAAVADRIRRYGIRNLVVDPVMIAKSGHPLLLPEAQAALRDLLVPLALVVTPNLPEAEALTGMAIRTRADMEAAGRRLLQMGARAAVVKGGHLGGGRAADLLIAADGSRLWLEADRVETRHTHGTGCTFSAAIAAGLARGLELPEAVSLAKEFITLAIRTAPGIGAGNGPTNHLAWLERGRRQPGEGGDRREHTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T60</accession>
    <name>Q67T60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194283.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH148"/>
    <dbReference type="EnsemblBacteria" id="BAD39133">
        <property type="protein sequence ID" value="BAD39133"/>
        <property type="gene ID" value="STH148"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH148"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_2_9"/>
    <dbReference type="OMA" id="SYQFRQP"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="99"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="36164" checksum="2C20A84AF490204A" modified="2004-10-11" version="1">MARYLTKRILNMIPLLIIVTLLSFGIMQLAPGGPEYVILSAEADAMSDPQKVEELREKWGLNDPIPVQYMRWVGNMLKGDFGKSYYYRLPVKEVLASALPNTIRLNLFALIFIYGISIPLGVISAVRQYSKADMLVTATAFAGQAAPSFWVASLLIWGVAMKSGGLIPTNGIATPGITLETHGWLTVFTDRAKYMLLPLVVIVFGGLTGLTRYMRSSMLEVLREDYVRTARAKGLAEKVVIYKHALRNALLPILAVSGGLLSGLVGGSVIIENIFSWPGIGRVAWQAVNNRDYNVSMSVMLLSAVLTMIGYLLVDIAYVWVDPRIKYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T91</accession>
    <name>FABZ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
            <ecNumber evidence="1">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
            <shortName evidence="1">(3R)-hydroxymyristoyl-ACP dehydrase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabZ</name>
        <name type="ordered locus">STH117</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194252.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T91"/>
    <dbReference type="SMR" id="Q67T91"/>
    <dbReference type="STRING" id="292459.STH117"/>
    <dbReference type="EnsemblBacteria" id="BAD39102">
        <property type="protein sequence ID" value="BAD39102"/>
        <property type="gene ID" value="STH117"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH117"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078912_3_0_9"/>
    <dbReference type="OMA" id="HFPHEPV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000091749" description="3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00406"/>
        </source>
    </evidence>
    <sequence length="142" mass="15449" checksum="06C314FD13289981" modified="2004-10-11" version="1">MEIIPHRHPFLLIDRVLELEPGRRVVAVKNVSMNEPVFQGHYPGNPIFPGVLILEAMAQAGAVAVLSQPEFAGKVPLFAGIDDARFRRPVLPGDQLRLEVEMVAMRRGLGVGKGMAYVGDELKAEATLKFALVDAATPEPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T98</accession>
    <name>Q67T98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polysulfide reductase subunit A, C-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH110"/>
    <dbReference type="EnsemblBacteria" id="BAD39095">
        <property type="protein sequence ID" value="BAD39095"/>
        <property type="gene ID" value="STH110"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH110"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747797_0_0_9"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="2">
        <location>
            <begin position="32"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="3">
        <location>
            <begin position="209"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="36940" checksum="3C48F84B98D4C2AD" modified="2004-10-11" version="1">MRFDGMGTKYPLSLGSIQAIPLVVESGKPYPLKAAIFFRVNPVKSSGDQRRWIEALKRLDLVVAIDTQMSETAMLAHYVLPEHHYLERMDAISVVGDTVSIRQPVVEPMYNTRSCLEILQGLAKVVGIEQYFNFTMEQWNNALLGPTGWTVKHLKEKGVIKVSATPPDYSKLPTSSGKAELVHKGFALSGGHEVVTWVPPKTQPEGDRLRLLHGHMAVHTNGYTQNVPALYARMPENDLWIHPTAAAARGIQDGDLVEVANEYGVQRIRARVTEGIRPDCVWMCHGFGTMAPEQRLAYGKGAADGWFYPILVTPVSAALGQGDATVTVRKVGEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA6</accession>
    <name>Q67TA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194237.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH102"/>
    <dbReference type="EnsemblBacteria" id="BAD39087">
        <property type="protein sequence ID" value="BAD39087"/>
        <property type="gene ID" value="STH102"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH102"/>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1133136_0_0_9"/>
    <dbReference type="OMA" id="FEVRMRG"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="2">
        <location>
            <begin position="143"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="24949" checksum="4336D1092BAB55E9" modified="2004-10-11" version="1">MKPVRGWPALWRGDLPPERRMALAAAAVLFLLIGGMAWYQMRPAPDAWPAPDAVGSADPAGFPEVTDSVDAAAAGSRSGSALRAGTGAASAAGDPRLTAGAGEAVADEPAEEAVAFSWPLSGRPRVVMAFGAVDGSLGDYRLSDGVALEATPGQPVTAAAAGTVALVEEHPVDGITVVLDHGGGKRSRYAGLAEAAVTEGQAVASGERLGRIGLPSPLRSDLGPHLLFGLMVDEEPVDPLPYLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE5</accession>
    <name>Q67TE5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative acyl-CoA hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH63</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194198.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH63"/>
    <dbReference type="EnsemblBacteria" id="BAD39048">
        <property type="protein sequence ID" value="BAD39048"/>
        <property type="gene ID" value="STH63"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH63"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149200_0_0_9"/>
    <dbReference type="OMA" id="YAGDYIE"/>
    <dbReference type="OrthoDB" id="1712258at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="143" mass="14978" checksum="ECA3FCB8DF06DDFE" modified="2004-10-11" version="1">MEATLRVRMSSKDAHYGGNLVDGARVLGLFGDVATELLIRHDGDEGLFVAYDSVTFKAPVYAGDFLEVRGRITRVGNTSRGMAFEAYKVIAADPTPEQPSRARVLEEPILVATATGTCVVPKPFQRGPGAGAGAAPTGDGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH4</accession>
    <name>Q67TH4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-dependent alcohol dehydrogenases and related dehydrogenases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH34</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194169.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH34"/>
    <dbReference type="EnsemblBacteria" id="BAD39019">
        <property type="protein sequence ID" value="BAD39019"/>
        <property type="gene ID" value="STH34"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH34"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826176_0_0_9"/>
    <dbReference type="OMA" id="GKMQNPV"/>
    <dbReference type="OrthoDB" id="1083526at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="35761" checksum="656A8CC98184777F" modified="2004-10-11" version="1">MTMGCPYGTHRVLEPAGSLPQPAWRLNDDPKIYDNEILIDVQRLNIDSASFRQISEEAGGDPDRIAAIIRRIVTERGKMHNPVTGSGGMLIGTVREVGPALNADVRPGDRIATLVSLSLTPLRIEAIHRVLPESDQVEVTGTAVLFETGIYAKLPDDIPDNLALAALDVCGAPAQTARLVKPGDRVLILGGGGKSGLLCSYAARRAGAAEVICFDYSDESLARARRLGAAHRYIQGDARDALGVMRAVGEKVDLTLSLVNVPGAELGAILATKPTGKIYFFSMATSFTAAALGAEGVGSQVEMLIGNGYYPGHADLTLNLLRESPELRALFEEIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ1</accession>
    <name>Q67TJ1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="3">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Spermidine synthase</fullName>
            <shortName evidence="3">SPDS</shortName>
            <shortName evidence="3">SPDSY</shortName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">speE</name>
        <name evidence="8" type="ordered locus">STH17</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194152.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH17"/>
    <dbReference type="EnsemblBacteria" id="BAD39002">
        <property type="protein sequence ID" value="BAD39002"/>
        <property type="gene ID" value="STH17"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH17"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_0_9"/>
    <dbReference type="OMA" id="HEMVAHP"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="PTHR11558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-methyl-5'-thioadenosine binding" evidence="3">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Spermidine binding" evidence="3">
        <location>
            <begin position="156"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="32648" checksum="E66DE6C12602D3A6" modified="2004-10-11" version="1">MELFLTENQTEDMRLSVRATRVLHHEKTPYQELLVVETPQWGRLMALDGFFQTCDRDEFVYHEMGAHVPLCTHPNPRRVLIIGGGDGGMAREAARHPEVERVDLVEIDERVVECCKQYFPQIAVALSGNPKVHVHIADGIQWVQEHEAEYDVVIIDSSEPIGPGEGLFTANFYARVFRCLKDDGILVAQTESPWVNAPVIQRAYRGIRQSFPITRLYTCAVPTYPTGLWSFTLGSKKYDPLQAEPEKRVGTLGELRYYTPEVHRAAFQLPVFVQRILAEAEAEEEQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-11-01" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q08897</accession>
    <accession>Q67KH1</accession>
    <name>TPL_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Tyrosine phenol-lyase</fullName>
            <ecNumber>4.1.99.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Beta-tyrosinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tpl</name>
        <name type="ordered locus">STH2842</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1993" name="Appl. Microbiol. Biotechnol." volume="39" first="341" last="346">
            <title>Cloning, nucleotide sequence, and overexpression in Escherichia coli of the beta-tyrosinase gene from an obligately symbiotic thermophile, Symbiobacterium thermophilum.</title>
            <authorList>
                <person name="Hirahara T."/>
                <person name="Horinouchi S."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="7763716"/>
            <dbReference type="DOI" id="10.1007/BF00192089"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-7; 270-279 AND 440-450</scope>
        <source>
            <strain>IAM 13621</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + L-tyrosine = NH4(+) + phenol + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21704"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15882"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="4.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text>Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-eliminating lyase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD41827" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.1.99.2"/>
    <dbReference type="EMBL" id="D13002">
        <property type="protein sequence ID" value="BAA02365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41827.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A48380">
        <property type="entry name" value="A48380"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_043714225.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q08897"/>
    <dbReference type="SMR" id="Q08897"/>
    <dbReference type="STRING" id="292459.STH2842"/>
    <dbReference type="EnsemblBacteria" id="BAD41827">
        <property type="protein sequence ID" value="BAD41827"/>
        <property type="gene ID" value="STH2842"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2842"/>
    <dbReference type="eggNOG" id="COG3033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047223_0_0_9"/>
    <dbReference type="OMA" id="VYTYAHM"/>
    <dbReference type="OrthoDB" id="91973at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050371">
        <property type="term" value="F:tyrosine phenol-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00543">
        <property type="entry name" value="Tyr_phenol_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011166">
        <property type="entry name" value="Beta-eliminating_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018176">
        <property type="entry name" value="Tryptophanase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013441">
        <property type="entry name" value="Tyr_phenol_ly"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001386">
        <property type="entry name" value="Trpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02618">
        <property type="entry name" value="tyr_phenol_ly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00853">
        <property type="entry name" value="BETA_ELIM_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000195638" description="Tyrosine phenol-lyase">
        <location>
            <begin position="1"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAA02365." evidence="2" ref="1">
        <original>A</original>
        <variation>R</variation>
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAA02365." evidence="2" ref="1">
        <original>D</original>
        <variation>H</variation>
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="458" mass="52163" checksum="77FD1321CCF089B2" modified="2004-11-23" version="2">MQRPWAEPYKIKAVEPIRMTTREYREQAIREAGYNTFLLRSEDVYIDLLTDSGTNAMSDRQWGALMMGDEAYAGARSFFRLEEAVREIYGFKYVVPTHQGRGAEHLISRILIKPGDYIPGNMYFTTTRTHQELQGGTFVDVIIDEAHDPQASHPFKGNVDIAKFEALIDRVGADKIPYINVALTVNMAGGQPVSMANLREVRKVCDRHGIRMWSDATRAVENAYFIKEREEGYQDKPVREILKEMMSYFDGCTMSGKKDCLVNIGGFLAMNEEWILQKAREQVVIFEGMPTYGGLAGRDMEAIAQGIYEMVDDDYIAHRIHQVRYLGEQLLEAGIPIVQPIGGHAVFLDARAFLPHIPQDQFPAQALAAALYVDSGVRAMERGIVSAGRNPQTGEHNYPKLELVRLTIPRRVYTDRHMDVVAYSVKHLWKERDTIRGLRMVYEPPTLRFFTARFEPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J45</accession>
    <name>Q67J45_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197435.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3324"/>
    <dbReference type="EnsemblBacteria" id="BAD42305">
        <property type="protein sequence ID" value="BAD42305"/>
        <property type="gene ID" value="STH3324"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3324"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1488314_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="181" mass="20243" checksum="0C32236CB28E88E7" modified="2004-10-11" version="1">MEHDERSAILKEADDLYARGNLEEALERYRRVLAEDETVAWAHSRTGAILAQLGDLDAAEVHLRRAIELDPKLPQAYSNLGNLDYSRGAYEAALEKYKKAVELDPDNPTFYENLHAAYKRLGKVYEAVEAIKKAQRLKRSQFRQEAGQDMANMSGSLKRRLGCLPTGVLLALAVLGLAICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J88</accession>
    <name>Q67J88_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B3_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197393.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3281"/>
    <dbReference type="EnsemblBacteria" id="BAD42262">
        <property type="protein sequence ID" value="BAD42262"/>
        <property type="gene ID" value="STH3281"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3281"/>
    <dbReference type="eggNOG" id="COG3382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076869_2_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B3_4" evidence="1">
        <location>
            <begin position="65"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="227" mass="24701" checksum="740713C0A1E89235" modified="2004-10-11" version="1">MLELQLKIDPELGRQLPFLTLGLLQIEGLENGPATAALQALADATVSQTRAALQLEEITRLPAISGWRKALKALGTDPARYRVSSERLLRRIVKGDGLPRVNLIVDLVNVWSAASGLPIGLYDADRLEGDTLAFGAGREGERYRTLAGTDMETLGKPVLRDAAGPCGSPITDSDRTATHEGTRRCIAVVFGPPLYDRDEFERHCDLLADWVRHFTTGRVTARGCVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J91</accession>
    <name>Q67J91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3278</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197390.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3278"/>
    <dbReference type="EnsemblBacteria" id="BAD42259">
        <property type="protein sequence ID" value="BAD42259"/>
        <property type="gene ID" value="STH3278"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3278"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_9"/>
    <dbReference type="OMA" id="TEMLIQD"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="34"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="354"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="387"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="612" mass="68737" checksum="41E69503B11342F8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J98</accession>
    <name>Q67J98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197383.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3271"/>
    <dbReference type="EnsemblBacteria" id="BAD42252">
        <property type="protein sequence ID" value="BAD42252"/>
        <property type="gene ID" value="STH3271"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3271"/>
    <dbReference type="eggNOG" id="ENOG5032YCW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2034049_0_0_9"/>
    <dbReference type="OrthoDB" id="1702657at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="125" mass="13962" checksum="18A3D17DD5A7095B" modified="2004-10-11" version="1">MRSRLLRLIYYGLPALVVAVSVLALNSGILLKRPSGEHDDVAGHLRLLLHHADTQRWEEARDAARKAGEAWLRMRGRIHLTSARDEVETFDLELAGLRGALETGDPVQARIAVHRLLALWEDLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA6</accession>
    <name>Q67JA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pyruvate ferredoxin oxidoreductase alpha-beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3262</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197375.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3262"/>
    <dbReference type="EnsemblBacteria" id="BAD42244">
        <property type="protein sequence ID" value="BAD42244"/>
        <property type="gene ID" value="STH3262"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3262"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020913_0_0_9"/>
    <dbReference type="OMA" id="GFFTSHQ"/>
    <dbReference type="OrthoDB" id="37118at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="POR_N" evidence="7">
        <location>
            <begin position="26"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="9">
        <location>
            <begin position="273"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="8">
        <location>
            <begin position="536"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="755" mass="83640" checksum="43425A329934A989" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE7</accession>
    <name>Q67JE7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH3221</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197334.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3221"/>
    <dbReference type="EnsemblBacteria" id="BAD42203">
        <property type="protein sequence ID" value="BAD42203"/>
        <property type="gene ID" value="STH3221"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3221"/>
    <dbReference type="eggNOG" id="COG2770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031034_0_0_9"/>
    <dbReference type="OMA" id="LHGMQER"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033463">
        <property type="entry name" value="sCache_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017204">
        <property type="entry name" value="Sig_transdc_His_kin_STH3221"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17203">
        <property type="entry name" value="sCache_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037433">
        <property type="entry name" value="STHK_STH3221_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="197"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="279"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="454"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="477" mass="52593" checksum="D24C98CA2CF4A5E2" modified="2004-10-11" version="1">MRRQEASGRPRLRVFGIRAKLISVLLALTIALGLVSILQLQATLPRTLREELDKRALSIARNVALRVTDPLLTANPLEVREILADVQATNPDVRYAFVLDSRGRLVAHTFAGGFPRDLLTQRPAAPDRTEDVQWLLSEEGVIHDATALIVDGLAGHVRVGLSEAHLMAMLRDMRRRQMLTLALACVLAVLLGYLGIWKVTVRVPQLVRAAQAVGRGDLTVRVRPGPADEFGHLAEAFNQMVRDLARTRALVVRKEAARKALLQKVLTAQEEERARISRELHDEVGQALTGLIVGLRVLEEGDAERQSQVTYLRDLAAGTLESVRRLSRDLRPAVLDDIGLVAAIRRYAGDFARYHGIEGTVQVVGDESVRLPPVVETTLYRITQEALTNVARHSQARHFGIVLDLRGPDVRLVIEDDGRGFDPGAPREGTGLTGISERLALVSGRMTIESSPESGTTLFIAVPREKEDESDAHLDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF8</accession>
    <name>Q67JF8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3210"/>
    <dbReference type="EnsemblBacteria" id="BAD42192">
        <property type="protein sequence ID" value="BAD42192"/>
        <property type="gene ID" value="STH3210"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3210"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064706_5_1_9"/>
    <dbReference type="OMA" id="LHASHNI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042150">
        <property type="entry name" value="MmRce1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35797">
        <property type="entry name" value="PTHR35797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="27129" checksum="4A264091904B0819" modified="2004-10-11" version="1">MVMAGSAAVVALGWHQLLMFVPALTALVMMLVIGRDGYTRDGWRRLGLGRAGVRRWPAALLLPAAALGGGYLAATAAGAAAPEPLPGAPGAARALNYLLLVAFYTFTFSLGEEVGWRGYLLPRLMAFGHRRGHLICGLAWAAFHYPVIFLSDLYLPDGNRLLGAGLFTLMVLPLSVVIGELRLRTDSLWVASLMHSAHNAFDEVVGSQVRMTAPLGPYLAGETGIVTLLIYLAAALWLLHRSGRRTGRAARSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH1</accession>
    <name>Q67JH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3197</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3197"/>
    <dbReference type="EnsemblBacteria" id="BAD42179">
        <property type="protein sequence ID" value="BAD42179"/>
        <property type="gene ID" value="STH3197"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3197"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_20_6_9"/>
    <dbReference type="OMA" id="PHAALWR"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="163" mass="18071" checksum="38C228E9A928E6EE" modified="2004-10-11" version="1">MEMAGVFRRERGCARMGRAYAGYPMPSCHALIRDGDRVLLVQRATPPLQGYWGLPGGRVELGETVEQALLREVREETGLQVDIERYLGYIDAIDRDEAGRVRYHYVVHYFTARPAGGSLRAADDAADARWVALSEVGGLPLTDAVQLCLNWDRQEAPPRAGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM3</accession>
    <name>Q67JM3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3145"/>
    <dbReference type="EnsemblBacteria" id="BAD42127">
        <property type="protein sequence ID" value="BAD42127"/>
        <property type="gene ID" value="STH3145"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3145"/>
    <dbReference type="HOGENOM" id="CLU_3048734_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="54" mass="6164" checksum="16843A4543058192" modified="2004-10-11" version="1">MFDLAYLLGYASPIPTPWYHGVRVSEATELLIVLTLFLGVLAAVYFIQRWIGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR9</accession>
    <name>Q67JR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Formate dehydrogenase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3099</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3099"/>
    <dbReference type="KEGG" id="sth:STH3099"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_1_2_9"/>
    <dbReference type="OMA" id="SELWFFY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047111">
        <property type="term" value="F:formate dehydrogenase (cytochrome-c-553) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006443">
        <property type="entry name" value="Formate-DH-alph_fdnG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01553">
        <property type="entry name" value="formate-DH-alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0711">Selenium</keyword>
    <keyword evidence="4 10" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="9">
        <location>
            <begin position="43"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="10">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1021" mass="112969" checksum="A250FFD82D472637" modified="2008-02-26" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS9</accession>
    <name>RL1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplA</name>
        <name type="ordered locus">STH3089</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197204.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JS9"/>
    <dbReference type="SMR" id="Q67JS9"/>
    <dbReference type="STRING" id="292459.STH3089"/>
    <dbReference type="EnsemblBacteria" id="BAD42071">
        <property type="protein sequence ID" value="BAD42071"/>
        <property type="gene ID" value="STH3089"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3089"/>
    <dbReference type="eggNOG" id="COG0081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_0_0_9"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="1671455at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125757" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <sequence length="235" mass="25035" checksum="43427844979B3A14" modified="2004-10-11" version="1">MPKHGKKYRSAAALVDRNKLYDPMEALELVKKTATANFDETIEVAYRLGVDVRHADQQIRGAVVLPGGTGKEVKVLVFAKGEKAKEAEAAGADYVGAEDLVAKIQEGWLDFDVAVATPDMMSLVGRLGRILGPRGLMPNPKTGTVTFDVANAIQEIKAGKVEYRTDKAGIVAAPIGKASFDVERLAANFRALTEALLRAKPAAAKGQYMKSVTISATMGPGIKVNPARLTATAEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW1</accession>
    <name>RL30_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmD</name>
        <name type="ordered locus">STH3057</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197172.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW1"/>
    <dbReference type="SMR" id="Q67JW1"/>
    <dbReference type="STRING" id="292459.STH3057"/>
    <dbReference type="EnsemblBacteria" id="BAD42039">
        <property type="protein sequence ID" value="BAD42039"/>
        <property type="gene ID" value="STH3057"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3057"/>
    <dbReference type="eggNOG" id="COG1841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131047_2_1_9"/>
    <dbReference type="OMA" id="ALFQIAH"/>
    <dbReference type="OrthoDB" id="2084745at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_B">
        <property type="entry name" value="Ribosomal_L30_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="PTHR15892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002211">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000056122" description="50S ribosomal protein L30">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <sequence length="60" mass="6497" checksum="D46CF6DC610220C0" modified="2004-10-11" version="1">MAGTLKITLKKGLAGRDQRIIATLKSLGLTKLNKTVERPDNPAVRGMIARVAHMVEVQEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW2</accession>
    <name>RL15_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplO</name>
        <name type="ordered locus">STH3056</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197171.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW2"/>
    <dbReference type="SMR" id="Q67JW2"/>
    <dbReference type="STRING" id="292459.STH3056"/>
    <dbReference type="EnsemblBacteria" id="BAD42038">
        <property type="protein sequence ID" value="BAD42038"/>
        <property type="gene ID" value="STH3056"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3056"/>
    <dbReference type="eggNOG" id="COG0200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055188_4_2_9"/>
    <dbReference type="OMA" id="FKNINRV"/>
    <dbReference type="OrthoDB" id="1874945at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104835" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="155" mass="16520" checksum="2D3222DDF295E591" modified="2004-10-11" version="1">MKLHELAPNPGARHERKRVGRGIGSGLGKTSGRGHKGQKARSGGGVRPGFEGGQMPLTRRLPKRGFSNAPFKVRYEVVNVADLERFEAGTVVTPELLDETRVCKNATKGVKVLGTGELTKALTVRAHAVSESARQKIEAAGGKVEVIGEATEEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY3</accession>
    <name>Q67JY3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197149.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3034"/>
    <dbReference type="EnsemblBacteria" id="BAD42017">
        <property type="protein sequence ID" value="BAD42017"/>
        <property type="gene ID" value="STH3034"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3034"/>
    <dbReference type="eggNOG" id="COG3874">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115880_1_1_9"/>
    <dbReference type="OMA" id="MDSFITT"/>
    <dbReference type="OrthoDB" id="1904616at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014229">
        <property type="entry name" value="Spore_YtfJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39162">
        <property type="entry name" value="PTHR39162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09579">
        <property type="entry name" value="Spore_YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021377">
        <property type="entry name" value="YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="12569" checksum="4E31127966351387" modified="2004-10-11" version="1">MAAEMLNSVMATLEKHLHIKSIVGEPMTVGDVTLIPVMDLTFGFGAGGGEGRDEKQGGSGVGGGGGARLAAKAVIVIKDGEVSVLPLGRGTAIDKIMEKIPGILEKVNVNVNAGGKEKDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K06</accession>
    <name>Q67K06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetyl-CoA acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197126.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3011"/>
    <dbReference type="EnsemblBacteria" id="BAD41994">
        <property type="protein sequence ID" value="BAD41994"/>
        <property type="gene ID" value="STH3011"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3011"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_9"/>
    <dbReference type="OMA" id="DYYWGMG"/>
    <dbReference type="OrthoDB" id="330994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_N" evidence="6">
        <location>
            <begin position="5"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="7">
        <location>
            <begin position="268"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="392" mass="41060" checksum="5468C027F98D08ED" modified="2004-10-11" version="1">MREAVIVTGVRTALGKAPRGSLRHVRPEDLAATVIKEAIARTPGLDPAAVEDVVLGCAFPEGEQGLNIARNAAIRAGLPTSVPGFTINRFCSSGLNAIAIAANAVMAGNLDIAIAGGVESMSRVPMGGHTPRPHPEVVESYPQLYMSMGHTAEVVCARYNVSREDMDAFALASHRKAAAAIDAGKFKDEIVPITVKERVFDGKRVVEKEFVFDTDEGVRRDTSMEALAKLRPAFSANGNVTAGNSSQTTDGAAAVVVMSAEKAQEMGLKPLAIFRSFAVGGVDPEVMGIGPVVAVPKALKQAGITLADVDLIELNEAFASQSLAVIRKLEMDPDKVNVNGGAIALGHPLGCSGARLTVTIMHEARRRKARYGLVTMCVGGGMGAAGVLEFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K50</accession>
    <name>Q67K50_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2965</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2965"/>
    <dbReference type="EnsemblBacteria" id="BAD41948">
        <property type="protein sequence ID" value="BAD41948"/>
        <property type="gene ID" value="STH2965"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2965"/>
    <dbReference type="eggNOG" id="COG2077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_12_0_9"/>
    <dbReference type="OMA" id="DICETTV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03014">
        <property type="entry name" value="PRX_Atyp2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002065">
        <property type="entry name" value="TPX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018219">
        <property type="entry name" value="Tpx_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01265">
        <property type="entry name" value="TPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="66"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="217" mass="23275" checksum="DC427A335FC2D847" modified="2004-10-11" version="1">MCLLQGIDAAAPAAVTGTLRRDPSRPVRPERPNTGKPTTPLLEGNAVERAGAFQFKGEVTLVGPELRVGDQAPAFTAIGNDLSTVDSRTFAGKVRIISSVPSLDTGVCDTQTRKFNEAAASLGDEVVVLTISADLPFAQKRWCGNAGVDRVITLSDYRDHAFSKAYGTYIKEWALCSRAVFVVDRNDRLVHVEYVPVAGQEPNYDAALEAARAALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K59</accession>
    <name>Q67K59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2956"/>
    <dbReference type="EnsemblBacteria" id="BAD41939">
        <property type="protein sequence ID" value="BAD41939"/>
        <property type="gene ID" value="STH2956"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2956"/>
    <dbReference type="HOGENOM" id="CLU_3277684_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="41" mass="4399" checksum="8FAB94E9EF63FA9C" modified="2004-10-11" version="1">MRARFNPAVITAVAMVALFAGVWLVGYLAGFSGEPETFLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K71</accession>
    <name>Q67K71_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2944"/>
    <dbReference type="EnsemblBacteria" id="BAD41927">
        <property type="protein sequence ID" value="BAD41927"/>
        <property type="gene ID" value="STH2944"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2944"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_9"/>
    <dbReference type="OMA" id="MKNFRDD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="12740" checksum="CB565E830018A18E" modified="2004-10-11" version="1">MNNSADLRREPTRPADREALARTLLRNVYDPEIGLNIIDLGLVYDLQVDEQDRCRVLMTFTTAGCPVGGHLVNEVYEALTVLGFADVKVDITFDPPWSPQRMSPEARQQLGWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K96</accession>
    <name>Q67K96_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2919"/>
    <dbReference type="EnsemblBacteria" id="BAD41902">
        <property type="protein sequence ID" value="BAD41902"/>
        <property type="gene ID" value="STH2919"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2919"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068892_1_1_9"/>
    <dbReference type="OMA" id="PIGYPGI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="46"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="33196" checksum="5958624F29F8E358" modified="2004-10-11" version="1">MEILQAIQTIQSPLLDRVLGLITNLHHEAVYILVLPLIYWLYDKRFGRYMFSVFVIGFWSNDFLKEVFRTPRPDPAQVRVILAETGGGYAFPSGHAQTPLVFWGAIADHVRRRWFTWAAGILVFLIGFSRLYIGVHWPLDVIGGWAIGLVVLWLMVRSRRFWTGEGQSLGLQALLAFLLPGAALGLLALLGPVDEIAWMVMGTWSGLLLGSALEEQWVRFDPRAGTPLQHLLKIIVGIVLVLAAKEGLKLVLPDSGVGDMVRYGLVAFTATLVAPWLFARFIARPPAVQLEQKTHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE8</accession>
    <name>Q67KE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine reductase complex C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2865</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196984.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2865"/>
    <dbReference type="EnsemblBacteria" id="BAD41850">
        <property type="protein sequence ID" value="BAD41850"/>
        <property type="gene ID" value="STH2865"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2865"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_327526_0_0_9"/>
    <dbReference type="OrthoDB" id="97643at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045984">
        <property type="entry name" value="DUF5940"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19364">
        <property type="entry name" value="DUF5940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="2">
        <location>
            <begin position="253"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5940" evidence="3">
        <location>
            <begin position="345"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="890" mass="93738" checksum="826AADDDC7FAA396" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH2</accession>
    <name>Q67KH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2841</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196960.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2841"/>
    <dbReference type="EnsemblBacteria" id="BAD41826">
        <property type="protein sequence ID" value="BAD41826"/>
        <property type="gene ID" value="STH2841"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2841"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030184_0_0_9"/>
    <dbReference type="OMA" id="PYLWFAK"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="410"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="543"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="622" mass="64897" checksum="BBEE5AC74AA2100A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK2</accession>
    <name>Q67KK2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2811"/>
    <dbReference type="EnsemblBacteria" id="BAD41796">
        <property type="protein sequence ID" value="BAD41796"/>
        <property type="gene ID" value="STH2811"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2811"/>
    <dbReference type="eggNOG" id="COG3324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1069303_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="140"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="27867" checksum="4A6BCD47C8E7740C" modified="2004-10-11" version="1">MEALFAPRLGGVFSADIAVPEHERELRFYARVLRTGPNPLWCEDLMNNLGIPIIGLGPRMPEYAHLPLQWMPHIQVADVAASVRRALDLGGRALMHQKDAAGNSQWAVLQDPNGAAFGVIPVIPREAIPPVPPAAGPVGRIARLDLTVSDAAAARDFYREVIGWSVQDVPVEDGDGRYADYAMLGGDGQGAATIRHARGPNAGLPPVWMLYLTVGDIAESLRRAEAEGGRVIKEVRDRDGAYVCAAIQDPVGVCFGLVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM1</accession>
    <name>Q67KM1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase_S49 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2792"/>
    <dbReference type="EnsemblBacteria" id="BAD41777">
        <property type="protein sequence ID" value="BAD41777"/>
        <property type="gene ID" value="STH2792"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2792"/>
    <dbReference type="eggNOG" id="COG0616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_2_9"/>
    <dbReference type="OMA" id="YVYDEIR"/>
    <dbReference type="OrthoDB" id="1318587at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269246" description="Peptidase_S49 domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="3">
        <location>
            <begin position="115"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="32387" checksum="20246D0B3C679367" modified="2004-10-11" version="1">MKNTMKRWIAAGAIVAIVVLSLAVAFWQGTGREVGSPKGPSGGQVALVRVEGTIVSGEGSGSLLSGAGAGSETIVKHLDRAAEDPAVKAVVLRVNSPGGSVVASWEIAEAVRRVQDAGKPVVVSMGESAASGGYWISAGADRIIASPDTMTGSIGVILQVGNLSEVYEKVGYKTYTFKSGPFKDMGSPDREMTDAERDLLQDLVDETYEAFVPVVAEGRGMDEAQVRKIADGRILTGRKALELGLVDELGDLKRAVQVAAELAGLPGEPEVREMNRANGLLGALLGRGILPPASLGLPSGLYALEPGPVSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS4</accession>
    <name>Q67KS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2738</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196857.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2738"/>
    <dbReference type="EnsemblBacteria" id="BAD41723">
        <property type="protein sequence ID" value="BAD41723"/>
        <property type="gene ID" value="STH2738"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2738"/>
    <dbReference type="eggNOG" id="COG1279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_4_0_9"/>
    <dbReference type="OMA" id="FARPRAW"/>
    <dbReference type="OrthoDB" id="1953934at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="23720" checksum="468DAFFA50685ADC" modified="2004-10-11" version="1">MGTALLHGLVLSFGLILPLGAQNTFVLTQGALHRRWTGALPSVLTAAVSDTLLITLAVSGLSLVLLTVPWLQMALQWCGVLFLLYMGWSTWRAAAAVDRDSGGTPGTARGAAPGGGPSGPAGDAAPDEAWPARRQVAFAASVSLLNPHAILDTVAVIGTGALAYTGTARLAFSLACIAVSWVWFFGLSLAGHLMGVLAAGANLQRWLIRASAVIMWGVALQLLRSLLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS5</accession>
    <name>Q67KS5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2737"/>
    <dbReference type="EnsemblBacteria" id="BAD41722">
        <property type="protein sequence ID" value="BAD41722"/>
        <property type="gene ID" value="STH2737"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2737"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_6_9"/>
    <dbReference type="OMA" id="AFHHAVN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="19"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="31751" checksum="CA5872F485A18E21" modified="2004-10-11" version="1">MRALTIRPLPDRPFTAPRPVVLAHQGASGHAPSNTMEAFRLALEQGADILELDVHMTRDGVVVVSHDETIDRMSDGTGLIKEMTLAELRRYDFGYRFTPDGGLTYPYRGKGVTIPTLEEVLQAFPEVPVNIEIKQADPPMEAQLWELIQRYGAEDRVLVASFHGTVAKRWRDLAGDRVATSAPVEHMYLVAAHYLSHLDRLYAPAHDAFQVPVAQKAGPLTVRFDTERFLRMAERVNVAVHYWTINDEDEMRRLYQLGAHGIITDYPDRAVKVLRELGLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX1</accession>
    <name>Q67KX1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Two-component response regulator variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2690"/>
    <dbReference type="EnsemblBacteria" id="BAD41675">
        <property type="protein sequence ID" value="BAD41675"/>
        <property type="gene ID" value="STH2690"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2690"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_3_9"/>
    <dbReference type="OMA" id="TRIMNEV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="106"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="309" mass="34851" checksum="92E359883A4B4F46" modified="2004-10-11" version="1">MRPAPTFSCRSHTARCACWKPSRNCSRRRRAKPMHDDIAGLTRAELEREVRYWRTQSLLLARDFGGVRADLSRQAAEVGLLRAQLLSYAEDLNREYQLVRERTVALERLLVATVGALANSIEARDPYTRGHTDRVARITLAFCERLGWERERLSIARMGALLHDIGKIGVPDAILRKPGRLTAEEYELMKQHTTIGAQILQGIDALVPAIPFVLCHHERWDGKGYPQGLAGEEIPIEGRILAIVDAFDAMTSVRVYRAPLTMETAVNEIRRCAGTQFDPALVPAFVRLYQDGLIHQALEEKGTRELYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L46</accession>
    <name>Q67L46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196737.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2615"/>
    <dbReference type="EnsemblBacteria" id="BAD41600">
        <property type="protein sequence ID" value="BAD41600"/>
        <property type="gene ID" value="STH2615"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2615"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_562483_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="485" mass="52548" checksum="A6ABA8A2FF41A80E" modified="2004-10-11" version="1">MTDKRFSPDEQAFLTETGLKAGDLAPLVADAPPLPDDVLARIRGRAREKAGLAHAAAAAPAAAPPDVPTASRRPRRWLVAAAAALLLVGGTLALSNPEGALAAIHRLVRLVPGIGLTETDGETWILPEPVSVEQDGVRVTVTGAISNRDGTQVRYRVDWPADEPLDKVEFASARESAFPELRLPDGTVIRRWGGSLAGGTRDMVGQYNFGPLPPGTSEITLVLPYLSGVAEPVEIPVTLVNAEEAGLAGAHPGNWSEERLGVQVGVPYWTAVDDRIVVSLDTRLPDGTRVIDYRDWIEPHGFVEPVLIDDQGHTYPLIIDEADRSGPWRRAVFQGPLAPDARQLTLTVPYLILADYTAEARLTVPLEQLAEGKPLRLNEELQIGSHRFTVETVTRIDADTFGFTLDLGPEEGGVLLQKVGIRHRMFGGPRAWSAHGENGQFTSMEVDFRSAPRRNLVIVFADPGYRVHGEWQVELPVPAGRSDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L97</accession>
    <name>Q67L97_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">VKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH2564</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VKOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196686.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2564"/>
    <dbReference type="EnsemblBacteria" id="BAD41549">
        <property type="protein sequence ID" value="BAD41549"/>
        <property type="gene ID" value="STH2564"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2564"/>
    <dbReference type="eggNOG" id="COG4243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146740_0_0_9"/>
    <dbReference type="OrthoDB" id="1932901at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12916">
        <property type="entry name" value="VKOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012932">
        <property type="entry name" value="VKOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044698">
        <property type="entry name" value="VKOR/LTO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038354">
        <property type="entry name" value="VKOR_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07884">
        <property type="entry name" value="VKOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00756">
        <property type="entry name" value="VKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="VKc" evidence="11">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD41549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="15655" checksum="A062DB8EA689984C" modified="2004-10-11" version="1">MSLQRLLRTIGVLALVGLGISLYLTWVYTTDRVAICLGSGGCETVQYSPYAWILGIPIPTLGAGAYLMVLALAVLGLRGSQPAEWVVLALFGTALVGLLFSAYLTYLELFVIHAICLWCAVSAVIQLFLFTLAVVVWRRFQEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB4</accession>
    <name>Q67LB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196670.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2547"/>
    <dbReference type="EnsemblBacteria" id="BAD41532">
        <property type="protein sequence ID" value="BAD41532"/>
        <property type="gene ID" value="STH2547"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2547"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114835_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="23147" checksum="78986D7709E1A300" modified="2004-10-11" version="1">MLESYTIRDIRQLKALSHTVRVRILAALAEKPMTAKQLADLFGEEPAKTSYHVKQLLKVGLVELKFTRETQNGIVEKYYQAIAREFQTDPLLTTQPGGRSRLEPALVRELNQVQSDFLRAYRADREQVGADGQAGGPQDPADGSAVHHGLHRLRLNAGQREAFLRELRDLIARYEDAEGDYAFHFLAYPHPHQAGAPGPVTSARGHT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-01-04" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG6</accession>
    <name>UPPP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppP</name>
        <name type="ordered locus">STH2495</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LG6"/>
    <dbReference type="SMR" id="Q67LG6"/>
    <dbReference type="STRING" id="292459.STH2495"/>
    <dbReference type="EnsemblBacteria" id="BAD41480">
        <property type="protein sequence ID" value="BAD41480"/>
        <property type="gene ID" value="STH2495"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2495"/>
    <dbReference type="eggNOG" id="COG1968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060296_1_0_9"/>
    <dbReference type="OMA" id="WEFLATG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0046">Antibiotic resistance</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000151222" description="Undecaprenyl-diphosphatase">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <sequence length="276" mass="29199" checksum="6C1B484FCF3E1EC9" modified="2004-10-11" version="1">MLFIGKTTEGFPVTVLQAVILGIVQGLAEFLPISSSAHLILVPWFLGWSPSGLAFDLALHVGTLIAVTIYFWRDLLNLAVEGLTKGMKTPTGRLAWGIVLGTVPGAIFGLLMEDVVEAVFRQSILTIALLLAVVGVILYLADRFGPKRRSLEEIRVSDVIWIGLAQALAVIPGVSRSGATITAGLALGLKRETAAKVSFLLGWPIILGGAVLVIPDADPAMFTPSFFAGVAAAAVSGYAVIAALLRYLQRGSYLVFAWYRGLVAAVTVALLLARGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL1</accession>
    <name>Q67LL1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Alpha-galactosidase/6-phospho-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="8">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2450"/>
    <dbReference type="EnsemblBacteria" id="BAD41435">
        <property type="protein sequence ID" value="BAD41435"/>
        <property type="gene ID" value="STH2450"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2450"/>
    <dbReference type="eggNOG" id="COG1486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045951_0_1_9"/>
    <dbReference type="OMA" id="IDMLCDG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019802">
        <property type="entry name" value="GlycHydrolase_4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001088">
        <property type="entry name" value="Glyco_hydro_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022616">
        <property type="entry name" value="Glyco_hydro_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32092">
        <property type="entry name" value="PTHR32092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02056">
        <property type="entry name" value="Glyco_hydro_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11975">
        <property type="entry name" value="Glyco_hydro_4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00732">
        <property type="entry name" value="GLHYDRLASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01324">
        <property type="entry name" value="GLYCOSYL_HYDROL_F4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_4C" evidence="9">
        <location>
            <begin position="214"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="451" mass="49621" checksum="D4815988E77B20E7" modified="2004-10-11" version="1">MAGAGEVSGKGWHATPQAREGDRMKLTIIGGASAYTPDIILGLLQDHALYAGGELCLHDIDGANLRVIERLARALVRAAGADLRVTATLDRAEAITGSRFILTQPRVGGLQHRALDEKIPLKHGLIGQETLGLGGFAFAWRTIPVMLEIVEEVQRLAPEAWIINYANPAGMVTEAVIRRFPDARFIGLCDMPTGLQWAIGRLLRVDYRRIALDYAGINHGGWVSRVLLDGEDVTARLRRWAAALGPVASLLPLGEQTGTVRLFRQHGMVPDPYLRYYYYKDQILKKLLRAERTRAEFLIERVQQLYRHYADVAGQEQPVLKEHRGHPAHSDLASQVIAAMAANRPTRFVIQQRGAGAVRHLPPEEAAQFSAIVDGSGWTPIPQPALPRKEAELIARIKASETLNVRAAMEGDRSLAVEAAELNPLGAPRPLAEKVVDELLAAHRKYLPQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM6</accession>
    <name>Q67LM6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oligoendopeptidase F</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Has oligopeptidase activity and degrades a variety of small bioactive peptides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196558.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2435"/>
    <dbReference type="EnsemblBacteria" id="BAD41420">
        <property type="protein sequence ID" value="BAD41420"/>
        <property type="gene ID" value="STH2435"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2435"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_1_1_9"/>
    <dbReference type="OMA" id="TELAPHM"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09609">
        <property type="entry name" value="M3B_PepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034009">
        <property type="entry name" value="M3B_PepF_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004438">
        <property type="entry name" value="Peptidase_M3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00181">
        <property type="entry name" value="pepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="8">
        <location>
            <begin position="112"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="7">
        <location>
            <begin position="206"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="597" mass="66999" checksum="AA599D4229F5E8D7" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM8</accession>
    <name>Q67LM8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2433</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196556.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2433"/>
    <dbReference type="EnsemblBacteria" id="BAD41418">
        <property type="protein sequence ID" value="BAD41418"/>
        <property type="gene ID" value="STH2433"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2433"/>
    <dbReference type="eggNOG" id="COG0641">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_3_3_9"/>
    <dbReference type="OMA" id="YPCYRFV"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024025">
        <property type="entry name" value="SCIFF_rSAM_maturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03974">
        <property type="entry name" value="rSAM_six_Cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="104"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="483" mass="53510" checksum="FA931F63F0727C12" modified="2004-10-11" version="1">MTIRRDTTGRDATVQHGEIHRFEMQGTRLVLDVHSGSLHLVDDVVWDLLEYGPEGPDEAGLERLRARHGAAGVEEALAEIAELRAQGLLFAPPPEWEPVMPKPGDPLRAICLNVAHACNLRCTYCFADDGTYGGPVKLMPFEVARQAVDLLIRLSGPRPVCEVDFFGGEPLMNWKVVRETIAYAREAGRRAGKAFTFTLTTNATLLTPEILDELDREQVSLILSLDGRPEVHDAKRSGSSRRVEEAIRMVLERRAPGGKPVWEYGAAQGATGRGAYAVLRGTYTADNLDFAEDALYMVDRMHSPYFSLEPVVATPDAPYALREEHLPRLLAEYERLAAEVDRRRREGRPITFHHFAVEAETGPCLPRRVQGCGAGVQYLAVTPEGDLYPCHQFVGRERFRLGNVAEGVVEKELQARLAGCHIYTKESCPTCWARYYCSGGCHANADLLNGDIFKPESIGCALTKKRVECGLWLKAQALLDTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ3</accession>
    <name>Q67LQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HIT family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2408"/>
    <dbReference type="EnsemblBacteria" id="BAD41393">
        <property type="protein sequence ID" value="BAD41393"/>
        <property type="gene ID" value="STH2408"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2408"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_818679_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025365">
        <property type="entry name" value="DUF4269"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="PTHR46648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14091">
        <property type="entry name" value="DUF4269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="202"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="297"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="339" mass="37075" checksum="BC28E8DB7CBB20DD" modified="2004-10-11" version="1">MTCRAGRLERVINWVDLRSGTAAQRQAYAALQELRIMEVLAPYDPVLAGTFPLDLNVPGSDLDIICEVHDGEAFGRTLTEAYGHLPGFAVRSAVKHGLPAVICGFRYGDLPVEVFGQPMPSRENHAWRHMAAEARLLALAGPGAAEEIRRLKAGGLKTEPAFAEYFALPGDPYETLLALADAPDNELGGLVERARRTRAECLFCRIATGGEASRVYEDPYTLAFMNIRQANPGHVLVIPKRHVEQVFDLDSDLDSDLAGRLGQTIAAVSRAIRAAFGVTDLNVFQNNGEAAGQEIYHVHFHLFPRRPGDGLFQVYPGHLPPPQPRAVLDDLAGRIRAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS5</accession>
    <name>Q67LS5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196509.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2386"/>
    <dbReference type="EnsemblBacteria" id="BAD41371">
        <property type="protein sequence ID" value="BAD41371"/>
        <property type="gene ID" value="STH2386"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2386"/>
    <dbReference type="HOGENOM" id="CLU_2866165_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="6834" checksum="97C7033F4B95DC86" modified="2004-10-11" version="1">MRYNGAFISGMLAGLALGAILVVALTPQTRQPVMQGMSRMGKGMRRMWNDGMDAVADAMTGDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU7</accession>
    <name>Q67LU7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2364"/>
    <dbReference type="EnsemblBacteria" id="BAD41349">
        <property type="protein sequence ID" value="BAD41349"/>
        <property type="gene ID" value="STH2364"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2364"/>
    <dbReference type="eggNOG" id="COG3214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047003_0_1_9"/>
    <dbReference type="OMA" id="PMRGTLH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009351">
        <property type="entry name" value="YcaQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06224">
        <property type="entry name" value="HTH_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="409" mass="45100" checksum="1F0C7285EF13C7C3" modified="2004-10-11" version="1">MVGAGYTSFRKRKSPLPVGRGLFAFARVQAPPGGGTCPTREERSEMRDARCVQCERYAQNQWLGRERRTGGVVELARHLVGIQSQLRTTPDLAARARLAGARPGDVRRELEETRRLVRTWTVRGTLHVVAAEDLPLLWRALRPEWESRWSKYLDKHVTRVQREAAAAACLQILARGPATRAELLEGAQQILGCRDEWVAYLFSSWGGVLKDLAYAGRVVYGPERDGEVQFVRTEDWLDLPVADMDPDDALAVLLERYLRAYSPARIQDFAHWSGVSVRRAREALTRLGGRVAVRDGWLVPEGEGGGSCAPDRQAGGPGADAGCVRLLPKFDPFLLAHADKFYLDEDHYKAVFRAAADVAAVILSGGRVIGTWRAEGSRVVPALFGPVDDAAAAELAQEVEAVSAWLRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB1</accession>
    <name>Q67MB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative S-layer associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196322.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2197"/>
    <dbReference type="EnsemblBacteria" id="BAD41182">
        <property type="protein sequence ID" value="BAD41182"/>
        <property type="gene ID" value="STH2197"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2197"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_230721_0_0_9"/>
    <dbReference type="OMA" id="RTEHYLI"/>
    <dbReference type="OrthoDB" id="1639867at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025883">
        <property type="entry name" value="Cadherin-like_b_sandwich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12733">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269266" evidence="2">
        <location>
            <begin position="27"/>
            <end position="2244"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="3">
        <location>
            <begin position="429"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="1726"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="5">
        <location>
            <begin position="2051"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="5">
        <location>
            <begin position="2115"/>
            <end position="2175"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="5">
        <location>
            <begin position="2182"/>
            <end position="2244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="2244" mass="241422" checksum="3013AA9D0CFADC2A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB7</accession>
    <name>Q67MB7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl-carrier-protein) synthase III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196316.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2191"/>
    <dbReference type="EnsemblBacteria" id="BAD41176">
        <property type="protein sequence ID" value="BAD41176"/>
        <property type="gene ID" value="STH2191"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2191"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_2_1_9"/>
    <dbReference type="OMA" id="DLNQKCA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="2">
        <location>
            <begin position="116"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="1">
        <location>
            <begin position="255"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="344" mass="36742" checksum="5250C93F90256303" modified="2004-10-11" version="1">MDRQTTASAGPPAVSLLGVSVYMPDRVEPAEAIAEQAGLPREVVEAKFGLKAKRRAAPDEHVSDMAVRAARPLLDLVDPAAIDAVIYFGSPHKDYYVWMAAPKIQHELGLSSATAFEVAAVSAGCPIALNVARSLLIANPALRNVLLVGASRESYLIDYGNVRSRFMFNFGDGAAAALVGRGPGKARVLATAAVTDGSFHDFVRVPAGGSRHPASHETVAGRMHCFDVTDPAAMKERLDPVSAQRFIGVARAAAEQSGFRPEEIRFVGMLHTKRSLFDQVMAALGIPPERQVYLDEYGHMSAIDPLVALYEAERRALLAPGDLAVLLSAGTGYTWAATAIRWGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD0</accession>
    <name>Q67MD0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41163">
        <property type="protein sequence ID" value="BAD41163"/>
        <property type="gene ID" value="STH2178"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2178"/>
    <dbReference type="HOGENOM" id="CLU_2208715_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="12060" checksum="31A2E439E9076E96" modified="2004-10-11" version="1">MREDDGRYRATASLTNKSGAGVELLYDCGSLLHLWYPDARLQLDSGQICPAVHSMLFPAGETMQREFVFDAREQGFPDVVVVYSRSLNGSHRHALRAVLEPIRENDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG8</accession>
    <name>Q67MG8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2140"/>
    <dbReference type="EnsemblBacteria" id="BAD41125">
        <property type="protein sequence ID" value="BAD41125"/>
        <property type="gene ID" value="STH2140"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2140"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_9"/>
    <dbReference type="OMA" id="AHPEIGC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="21602" checksum="C07475661B1090C5" modified="2004-10-11" version="1">MLQSEPDLELVGEAGRGEEAVELTKALKPDVVIMDISLPDMSGIEATRLIKEAVPETRVIGLTMHEEEPFVLEFLRAGADAYIVKRSAAADLVGAIRAVMEGQAVLDPAVTRAVVEGYVSRPGRAVGQPEEPCPLTPREREILVLIAEGLTNAEIARRLYISEKTVQTHRSNMLDKLGIHDRTELVRYAIRQGLVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN4</accession>
    <name>Q67MN4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196199.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2074"/>
    <dbReference type="EnsemblBacteria" id="BAD41059">
        <property type="protein sequence ID" value="BAD41059"/>
        <property type="gene ID" value="STH2074"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2074"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_4_2_9"/>
    <dbReference type="OMA" id="FTLMQWR"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="26172" checksum="8FC2CC83F7822446" modified="2004-10-11" version="1">MWIVFRGLLSGALRDRISLFYALIFPAAALAVLAYFFEDPGYRRHLLVGVTALGGFFFTMSGTGFEVMRQRQRGVYKLLRATPFPTGAFVSALTAARGLIALVCALFVYVGGALFLGAGIAPAGLLLMLPALALGTVCFTALGFILGNLGNTETQVAGINNLFVLPMTCASPVFYSLEGAPRWVQLLSEAMPLRHLVAALQAAAAVDPVAMIRPLAILCGFTAAILLVAVATFRWDPDARLMRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN6</accession>
    <name>Q67MN6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AraC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2072"/>
    <dbReference type="EnsemblBacteria" id="BAD41057">
        <property type="protein sequence ID" value="BAD41057"/>
        <property type="gene ID" value="STH2072"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2072"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_1_9"/>
    <dbReference type="OMA" id="HLRRMFS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010499">
        <property type="entry name" value="AraC_E-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00871">
        <property type="entry name" value="AraC_E_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="32964" checksum="549C11D018D13759" modified="2004-10-11" version="1">MVGHEGGHDVNPLHDLNGALDYIEAHLDGEVDIRAAARIAGCSEYHFRRMFATLAGMPISAYVRRRRLTLAAQELTAGARVTDVALKYGYDSPDAFARAFQAEHGFPPSQARLPGRALNAFSRMAFQLTIRGGTDMRYRIVEKEPFRIVGIMRRVRLQYEGVNPEIAAMWKDLGMEQIQELIALSNVEPKGIIQASLNFSEGRADGGSLEHWVGAATDQPCPPGFQALEVPALTWAVFESVGPFPETLQSTWARIYAEWFPSSGYQAAEGPEILWNETDDVSSPEFRSEIWIPVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ4</accession>
    <name>Q67MQ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase similar to anaerobic dimethyl sulfoxide reductase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196180.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2054"/>
    <dbReference type="EnsemblBacteria" id="BAD41039">
        <property type="protein sequence ID" value="BAD41039"/>
        <property type="gene ID" value="STH2054"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2054"/>
    <dbReference type="eggNOG" id="COG3302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064909_1_1_9"/>
    <dbReference type="OrthoDB" id="909963at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019645">
        <property type="term" value="P:anaerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007059">
        <property type="entry name" value="DmsC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38095">
        <property type="entry name" value="PTHR38095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04976">
        <property type="entry name" value="DmsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="33082" checksum="E3F7330B0C59E726" modified="2004-10-11" version="1">MAAWLHEAQYSLVIFTVLIQLTVGALWALAAVDLRLAGAADPARSRFTRLGTAVLVPAAAVGLLFSTTHLGRPLLAVRALRHWETSWMSREIWATGLFFGLIALYTLLWWWRPSADKARRAVGAAGAAVGALTVVTQAMIYLIPGRPMWNHWSTVVLFFGSAFTLGPLAVAAAQGLAAGLAGSGADEVLRSGFGRLGSAVLGGAAVYALGLAGRLTYLSAGAAAALATAGAAGKAAVGADTVKTALLVARQAVEANGLLLGAEVALAIGAPAAVGAALWLICRRGGSLRLCRGLASFGMVLTLLGHLAGRALFYLTGQPWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS5</accession>
    <name>Q67MS5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196159.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2033"/>
    <dbReference type="EnsemblBacteria" id="BAD41018">
        <property type="protein sequence ID" value="BAD41018"/>
        <property type="gene ID" value="STH2033"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2033"/>
    <dbReference type="eggNOG" id="COG2345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078469_1_1_9"/>
    <dbReference type="OMA" id="LLNCPFH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="25828" checksum="3FAAC6ED9C06CE83" modified="2004-10-11" version="1">MERDPQKISAVLADPTRYSIYQYVLVAPDPVSAAEVARRFDLHPNVARMHLNRLTEVGLLRQRTEKTGRGGRPSYVYEPSGSAVSLTTVPPRDFQLLADLLVQSLALLGEGGKEAVEQIGHSFGRRLGQEALSKLAPSLNAANGATGPQALLEACAMALSRLGVSARVIKGPGDRPSLVLKSCGFHEVASAHPEQVCHLCKAMVEGVAQTCAEVPPAVTQAGTVPRGDLECVYEVDGLIRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV0</accession>
    <name>Q67MV0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196135.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2008"/>
    <dbReference type="EnsemblBacteria" id="BAD40993">
        <property type="protein sequence ID" value="BAD40993"/>
        <property type="gene ID" value="STH2008"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2008"/>
    <dbReference type="eggNOG" id="ENOG502ZA24">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_520484_0_0_9"/>
    <dbReference type="OrthoDB" id="1783261at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="425" mass="46317" checksum="43E247107FB53B12" modified="2004-10-11" version="1">MERIMDVVAEAHRIGGAGVLWPGFDLAAIPILLYGTEEAWLIGHPAPPEGYGHAGEVAGRPVYRGPVRPELAGNTAAPVGGHLTALVNLSDSPPTDPGALARLILHEAFHVFQQTAFPDLPRMDAAALQAMAAYPENDPANNAMAIVENRLLARALEGDPAAPGAFVSMRQHRHRQLVRMDRGDAVVYEQTVEWVEGTPTYVELKAGAPTDGLVERLQTHNLGGRHAAYRRFYDTGAAQALLLDRVAPGWQARIADAGGCLQRLLEESLTEPLPPVNQVVVAEGLAALLEAEQEAEAERQARIAALLRQLDEGPGLAVEIRLPPEVQGLMWDPTNLLTISPGRRLHTRFCGAVGPDGLRVTIHALCLEEWGERGRRFRLRLPGRPEVQRGGRLRIVTEALSVDAPYGHIEEEPGLMRVQLWLSRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-01-19" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N11</accession>
    <name>PSUG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pseudouridine-5'-phosphate glycosidase</fullName>
            <shortName evidence="1">PsiMP glycosidase</shortName>
            <ecNumber evidence="1">4.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psuG</name>
        <name type="ordered locus">STH1947</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribose 5-phosphate + uracil = H2O + psi-UMP</text>
            <dbReference type="Rhea" id="RHEA:18337"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="4.2.1.70"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine-5'-phosphate glycosidase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.70" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196074.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N11"/>
    <dbReference type="SMR" id="Q67N11"/>
    <dbReference type="STRING" id="292459.STH1947"/>
    <dbReference type="EnsemblBacteria" id="BAD40932">
        <property type="protein sequence ID" value="BAD40932"/>
        <property type="gene ID" value="STH1947"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1947"/>
    <dbReference type="eggNOG" id="COG2313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012201_0_1_9"/>
    <dbReference type="OMA" id="RKLGHRD"/>
    <dbReference type="OrthoDB" id="1294333at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046113">
        <property type="term" value="P:nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="PTHR42909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="SSF110581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000390552" description="Pseudouridine-5'-phosphate glycosidase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01876"/>
        </source>
    </evidence>
    <sequence length="308" mass="32442" checksum="FB19BF928EADF8DF" modified="2004-10-11" version="1">MKYLLSYTPEVREALDRNRPVVALESTIISHGMPYPENLRTAREVEEIVRSQGAVPATIAVIGGRCKVGLTDEELELLATSPEAVKVSLRDLPVVLARKSLGATTVATTATIAAAAGIEVFVTGGIGGVHRKSPGDPAQMWDVSADLTVLGRTDITVVCAGAKSVLDIGATLEVLETLGVTVLGYRTDRFPGFYTRDTGFGVDARVDTPEEAAAVIHARQQTMLPGGVLVVNPVPEEHAMDPDEVERHIADALKAMAAEGVTGKAVTPYLLARLKEVTSGRALQTNIALVKHNALVGAQIAVALKAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N51</accession>
    <name>Q67N51_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196034.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1907"/>
    <dbReference type="EnsemblBacteria" id="BAD40892">
        <property type="protein sequence ID" value="BAD40892"/>
        <property type="gene ID" value="STH1907"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1907"/>
    <dbReference type="HOGENOM" id="CLU_1730489_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="16520" checksum="343AB3F4B2381647" modified="2004-10-11" version="1">MLNHGIGFSLERLRLRPAAFWAAGAASAGLFVDWLLRVESFSPLDENSGKALLGLTFLAAGLFGWAVLIAIGEWALRQRIRSPWAPLAWGALLTLLTIGWFPPGAMLGSLYVGTLLADRLGATRRVPWPVVWPFWLAGSLGITFAILRFFV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N94</accession>
    <name>EFP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor P</fullName>
            <shortName evidence="1">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">efp</name>
        <name type="ordered locus">STH1864</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the elongation factor P family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195992.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N94"/>
    <dbReference type="SMR" id="Q67N94"/>
    <dbReference type="STRING" id="292459.STH1864"/>
    <dbReference type="EnsemblBacteria" id="BAD40849">
        <property type="protein sequence ID" value="BAD40849"/>
        <property type="gene ID" value="STH1864"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1864"/>
    <dbReference type="eggNOG" id="COG0231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074944_0_1_9"/>
    <dbReference type="OMA" id="WSVVEFQ"/>
    <dbReference type="OrthoDB" id="1260763at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094349" description="Elongation factor P">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <sequence length="185" mass="20644" checksum="19A7F04B9F70316C" modified="2004-10-11" version="1">MISVNDLRNGMTIEMDGTVYQVIEFLHVKPGKGAAFVRTKLKNILTGATIETTFRAGEKVEQANVDRREYQFLYADQGVWVFMNNETFEQIELTEEQVGNAPNFLLENMTVQIASWKGQVIGVDLPNTVELKVVETEPGFKGDTATGTYKPAKLETGYVVQVPLFVNTGDVIKVDTRTGEYLSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA2</accession>
    <name>Q67NA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage III sporulation protein AG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoIIIAG</name>
        <name evidence="1" type="ordered locus">STH1856</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195984.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1856"/>
    <dbReference type="EnsemblBacteria" id="BAD40841">
        <property type="protein sequence ID" value="BAD40841"/>
        <property type="gene ID" value="STH1856"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1856"/>
    <dbReference type="eggNOG" id="ENOG50330Z5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071454_2_1_9"/>
    <dbReference type="OMA" id="NVQIKQT"/>
    <dbReference type="OrthoDB" id="1762318at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014195">
        <property type="entry name" value="Spore_III_AG"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02830">
        <property type="entry name" value="spore_III_AG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="22147" checksum="F41BDDB7A51D7744" modified="2004-10-11" version="1">MGERKAPGNGPGSPWSWLGLGLSKEQAKLVPLIAVLLFIGILMLQSDELFGIDADSPPLDPAPGASLVGPVGAEDELTRLERQKAAELEEMLGQIEGAGRVRVMVTLAAGPAIQVVKNTTVDQSTTTEEAADSSTRRIESINTREDHVFTRSGSSEQPVIAQTSAPEIAGVLIVAEGARDVRIRARLLDAAMVALNVPANRIQVVPADGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND0</accession>
    <name>Q67ND0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphotransacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1828</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1828"/>
    <dbReference type="EnsemblBacteria" id="BAD40813">
        <property type="protein sequence ID" value="BAD40813"/>
        <property type="gene ID" value="STH1828"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1828"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056531_0_0_9"/>
    <dbReference type="OMA" id="CIVDGPF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012147">
        <property type="entry name" value="P_Ac_Bu_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000428">
        <property type="entry name" value="P_Ac_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTA_PTB" evidence="2">
        <location>
            <begin position="32"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="28884" checksum="726A91D3BB72AA2D" modified="2004-10-11" version="1">MITSLDQLIPAAQAAVGGRPPVMAVAQADDPHVEEAVSEAERLGLVRGYRISRADPAEAARLAVDAVRAGEAQLLMKGLLQTAEILRAVLNRDAGLRTGRALSHACVVQVPGFPRLLHISDIALNIRPDLARKADILRNQVAVARALGLECPKVAAIAAVEKVNPDMPATVDARALQEMAERGEFGDCVVQGPLALDLALSAEAGAVKGVTGPVVGAADVLIMPDLETGNVLYKALIHLAGAASAGIVVGARCPIVLLSRSDPPAFKLNSIALAVLAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF5</accession>
    <name>Q67NF5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Segregation and condensation protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">scpA</name>
        <name evidence="4" type="ordered locus">STH1803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Associated with two foci at the outer edges of the nucleoid region in young cells, and at four foci within both cell halves in older cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ScpA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1803"/>
    <dbReference type="EnsemblBacteria" id="BAD40788">
        <property type="protein sequence ID" value="BAD40788"/>
        <property type="gene ID" value="STH1803"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1803"/>
    <dbReference type="eggNOG" id="COG1354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038686_3_1_9"/>
    <dbReference type="OMA" id="YLVMAAM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01805">
        <property type="entry name" value="ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="29980" checksum="85F9F1BFBC71A8D6" modified="2004-10-11" version="1">MDRSTRPEPYVVHLANFEGPLDLLLHLIRREEMNIYDIPIARITEQYLAAIQDLADLDLDRASEFLVMAATLLRIKSQMLLPKPPRLPADEEGPEEPEEDPREALVRQLVAYSRFKEAAELLREREREMLRVYTRGLFVEEPGGPPPLEGLDLADLVRAFQEVLKEEWNWREVPRAEIPLREKIREIRFRLSRSPGGVRFRDLFARGCSRLEVVVTFLALLELVRRRQVRVVQQALFGEILVLQAEPPQDEAEGGREDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG3</accession>
    <name>Q67NG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195923.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1795"/>
    <dbReference type="EnsemblBacteria" id="BAD40780">
        <property type="protein sequence ID" value="BAD40780"/>
        <property type="gene ID" value="STH1795"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1795"/>
    <dbReference type="eggNOG" id="COG3285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_1_0_9"/>
    <dbReference type="OMA" id="TTAWWKE"/>
    <dbReference type="OrthoDB" id="2061599at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014145">
        <property type="entry name" value="LigD_pol_dom"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02778">
        <property type="entry name" value="ligD_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="307" mass="34013" checksum="0FCA19DB87BE5A61" modified="2004-10-11" version="1">MARTERRWVRVGGRELAVTNLEKPMWPADGLTKGDLISHYVSVAPYLLPYLRGRPVVLTRYPHGVGGKWFYQKDAPQGLPDWIPTWADRAEDGRVIRYLRIEEPAALAYVANLGAIELHPWLSSCDSPDHPDWAVIDLDPAEGATFADVLVVARLVRQILGEVQVQGFPKLSGATGVHIFCPAAPGTTYAETAAFCEQVGRILLRAYPEKVTLERAVARRGPKVYVDYLQNRRGQTITSVYGLRPLPGAPVSAPVTWEELEGEPPRITMRTLPARLAAVGDLFAPMLQATQDLQAATERLRRYLTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM8</accession>
    <name>Q67NM8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195858.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1730"/>
    <dbReference type="EnsemblBacteria" id="BAD40715">
        <property type="protein sequence ID" value="BAD40715"/>
        <property type="gene ID" value="STH1730"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1730"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049710_2_0_9"/>
    <dbReference type="OMA" id="LHFRITH"/>
    <dbReference type="OrthoDB" id="955123at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="6">
        <location>
            <begin position="80"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="439" mass="48167" checksum="E12C983D2705E285" modified="2004-10-11" version="1">MTPANQFLLDAAQWLLLGAFASYLVAFLLYVSGTLGRRISGGTRFTRTPGHGTVANVIGVVLHGLAILARWQGSGFWPTSNMYEFIGFMAFSSMVAFLVLHGMYRLYVLGALVTPVTIALLAYSYVFPPEVTPLIPALQSYWLPLHVSLAALGEGFFAVAFGAALLYLLRVRGMEIAQARAVSEAAAGMETAAPMAGAAPMAGAAPGVAAESRWERIWGVRLLEIVFYLILVLLGFTVLALFFRYAGFQWIFNNGMTTYHLPPIIGPYGAEVGEKGTILGIPLPTVVVPFGWRGKHLNTLLYSVVIGAFLYGFIRRFVTRGRIGDALALRVTADPELLDEISYRAVAIGYPIFTLGGLIFAMMWAKEAWGRYWMWDPKETWAFIAWLVYSAYLHFRITHGWEGRRSAWLAVLGFGVILFTLVGVNLLIVGLHSYAGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW0</accession>
    <name>Q67NW0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1648"/>
    <dbReference type="EnsemblBacteria" id="BAD40633">
        <property type="protein sequence ID" value="BAD40633"/>
        <property type="gene ID" value="STH1648"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1648"/>
    <dbReference type="eggNOG" id="COG2350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110355_8_0_9"/>
    <dbReference type="OMA" id="TVHEWNT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="97" mass="10577" checksum="BDCB03EB5D0BF1D2" modified="2004-10-11" version="1">METCYMIRLTRRPGCVITDELMRAHRAHLKRLAEQGRLVLCGPFADRTGGMTVIRAASLAEAEAVAAADPLVASGMEDCEIREWIITGGEGRPLEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ9</accession>
    <name>Q67NZ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1232 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195738.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1609"/>
    <dbReference type="EnsemblBacteria" id="BAD40594">
        <property type="protein sequence ID" value="BAD40594"/>
        <property type="gene ID" value="STH1609"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1609"/>
    <dbReference type="eggNOG" id="COG3339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_874144_0_0_9"/>
    <dbReference type="OrthoDB" id="2017850at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF1232" evidence="5">
        <location>
            <begin position="227"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="34422" checksum="91F94F6822C4D717" modified="2004-10-11" version="1">MLRATGVTLTLTVTDVEEALAGFAAGAVQDLRVGLESGAILVRLRVPVEPVRMLVPVELRFTVMAAAGNLVKLGVAWTNMGLLPAFVKEKALQKAFEALPGEYQDGVWRLDLGEVLEHVPVSFRLKGVHVDRDGIVVELADVMAFPIDTSALGALSPGALVPVPSAGEQTIPEHQGFYDKLRERVRRYAAEKAPQWAQPLIPWLMAVPDFFVLLVRLARDPRVPPAAKVIAGATVAYFVSPIDLIPDPIPLIGEVDDLGLALLAVEQIARLVPHDVVQEAWPGEGDVLQLVREGTNLITRVLPNRVLTALKKVLEQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA8</accession>
    <name>Q67PA8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc metalloprotease</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195630.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1500"/>
    <dbReference type="EnsemblBacteria" id="BAD40485">
        <property type="protein sequence ID" value="BAD40485"/>
        <property type="gene ID" value="STH1500"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1500"/>
    <dbReference type="eggNOG" id="COG0750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025778_1_3_9"/>
    <dbReference type="OMA" id="QYMVGFG"/>
    <dbReference type="OrthoDB" id="1395197at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00054">
        <property type="entry name" value="TIGR00054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="10">
        <location>
            <begin position="10"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="344" mass="37632" checksum="309D8CE2C5A879C4" modified="2004-10-11" version="1">MTLADWLWAIPVFGLMIFMHELGHFAVAKFFDIRVHEFALGFGPALVGFNRGETRYSLRAIPLGGFVRMAGMDPSEPDDPRGFNSKPIYQRALTIFAGPFMNFLLASLLLSGYIYAQGVPVSEPIFGDVLAECNGQPCPAAMAGLQKGDRVLTIGGSPVENWSDILTYVGTSEGAPLEIRFERDGQEMTTVLTPVYMDGRWMIGIQQATRPGSFWKALAQGPSITWEYSKAWVASLVQAVTGRTELELSGPVGITREIATQASAGLTNLLWLTAFLSINLGLFNLLPIPALDGSHLLFMAVEAVRGRRLDPERVNMVHFFGFLLLMGLILVVTYGDLRAAILGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH3</accession>
    <name>Q67PH3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1435</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195565.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1435"/>
    <dbReference type="REBASE" id="10269">
        <property type="entry name" value="M2.StpORF1434P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40420">
        <property type="protein sequence ID" value="BAD40420"/>
        <property type="gene ID" value="STH1435"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1435"/>
    <dbReference type="eggNOG" id="COG3392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034356_0_0_9"/>
    <dbReference type="OMA" id="IFCDLFA"/>
    <dbReference type="OrthoDB" id="51297at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012327">
        <property type="entry name" value="MeTrfase_D12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02086">
        <property type="entry name" value="MethyltransfD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00505">
        <property type="entry name" value="D12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="347" mass="39589" checksum="00A966DBA1384C20" modified="2004-10-11" version="1">MKPLWVQNRKYIGSKHNLLPFIEQVVAERAPEARSLADPFTGSAVVAYHFAARGLTVAAADNLYHNYIAARCFLGGRPGEVRWDRVAELIAHLNRLPPEQGYCWREYGGTYFTPENAGRIDAVREQIARWRQERIIGEQEEAVLLTSLIYAADKVANTCGQYDAFLKHLGAKPYAEDGTHLVDATVYKPLELGLPQVVESEANRVYCGDADHLIDRMEADVLYLDPPYNSRQYIDNYHVLENIARWEKPRLYGKTRKFARDGLKSAYSRRSTAGAHLTRLIQRARARHILLSYNNEGIIPDEVIRAALEQRGPVELFERRYAIFGNGAGRSGRRPIVERLFYCRVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH8</accession>
    <name>Q67PH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195560.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1430"/>
    <dbReference type="EnsemblBacteria" id="BAD40415">
        <property type="protein sequence ID" value="BAD40415"/>
        <property type="gene ID" value="STH1430"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1430"/>
    <dbReference type="HOGENOM" id="CLU_169738_2_1_9"/>
    <dbReference type="OMA" id="MMANQNG"/>
    <dbReference type="OrthoDB" id="2064087at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="7632" checksum="46C08E349CB7C935" modified="2004-10-11" version="1">MPGNNNSILVSQARAALEQMKNETAAELGIQNYAQQYKGDLPSRVNGSVGGYMVKKMIALAEQQLAGGTGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ7</accession>
    <name>Q67PJ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">trpS</name>
        <name evidence="10" type="ordered locus">STH1411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195541.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1411"/>
    <dbReference type="EnsemblBacteria" id="BAD40396">
        <property type="protein sequence ID" value="BAD40396"/>
        <property type="gene ID" value="STH1411"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1411"/>
    <dbReference type="eggNOG" id="COG0180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_1_1_9"/>
    <dbReference type="OMA" id="GWGQFKP"/>
    <dbReference type="OrthoDB" id="951354at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_B">
        <property type="entry name" value="Trp_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024109">
        <property type="entry name" value="Trp-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="37348" checksum="32C30106EB05D762" modified="2004-10-11" version="1">MSTKPIKVVFSGMQPTSGVTLGNYLGAMRNFVKLQHEAQCFFCVVDLHALTIPQDPVQLRKNTLDMARLYVAAGIDPDVATIFVQSHVSAHAEMGWLMEVSCYYGELQRMTQFKDKSAKAEVVTTGLFTYPALMAADILLYDTTHVPVGDDQKQHLELARDIAVRLNNRFGREVVVVPEPYIPPREAGGRIMSLQNPTQKMSKSDPDESATIHMLDTPDAIRKKIKRAVTDSESEVRYDPEKKPGVSNLMVILSLCTGMSLDEIAGRYTGYGQFKKDVAEAVVATLEPIQQRYKELCEPGVIEEILEKGAQKAEAVSTPVLRRLQDALGLLPRKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ8</accession>
    <name>Q67PJ8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallophos_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195540.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1410"/>
    <dbReference type="EnsemblBacteria" id="BAD40395">
        <property type="protein sequence ID" value="BAD40395"/>
        <property type="gene ID" value="STH1410"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1410"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_3_2_9"/>
    <dbReference type="OMA" id="LGDYPAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="54"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="29009" checksum="2D34835289EFD1E7" modified="2004-10-11" version="1">MKRRHFLVRAAVGLIGLTLGLTGYGRFVETRRVRFEERTAAVPGLPRHLEGYTIGVLSDLHVGPTTPAAWVRRVVEQLAERRPDLVVAVGDFIGSHDHVAEANWCLEPVQGALGVLGNWDYYDGRIRKALTNVRLLENRGVLVADGLWVGGVDEPALGKPDVDLALSGAPPGAVRILLCHEPDYADALVRPEHRVALMISGHSHGGQVRLPVVGPLILPPGGRKYHTGFYQAAHCRVYTSRGVGTAHLPIRLFCPPEVTLLRLVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP6</accession>
    <name>Q67PP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195492.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1362"/>
    <dbReference type="EnsemblBacteria" id="BAD40347">
        <property type="protein sequence ID" value="BAD40347"/>
        <property type="gene ID" value="STH1362"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1362"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_0_7_9"/>
    <dbReference type="OMA" id="SADFEMI"/>
    <dbReference type="OrthoDB" id="865276at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="27161" checksum="662DA17B184F9A54" modified="2004-10-11" version="1">MPDNPKVTVVIPAYNAARYLAEAVESALNQTYSNLEVLVVDDGSTDRTPFLLERYGRAIRTIRKRNGGTPSALNEGIRQARGEWIAWLSADDAFLPEKLAKQMAYAEAHPECALIYTNWFVVDGRGKTVSHLASPTFRSRADQVEKLLRGCVINGSTTLVRRDVYLRAGLFDESLPQAHDWDMWLRLARDYRFGHVAEPLLRYRWHGENLSARPDALAYNDRVLAKARAYLQGWLPGGGGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX6</accession>
    <name>Q67PX6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1282"/>
    <dbReference type="EnsemblBacteria" id="BAD40267">
        <property type="protein sequence ID" value="BAD40267"/>
        <property type="gene ID" value="STH1282"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1282"/>
    <dbReference type="HOGENOM" id="CLU_206044_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="7284" checksum="00C8BC88FBF1FFC6" modified="2004-10-11" version="1">MLTFLLWLLLLVIAPPVALVALLLYPLVWLILLPFRIVGIAVDGVLRLVWAVVTLPARILTRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY1</accession>
    <name>Q67PY1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PTS system mannose-specific IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195408.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1277"/>
    <dbReference type="EnsemblBacteria" id="BAD40262">
        <property type="protein sequence ID" value="BAD40262"/>
        <property type="gene ID" value="STH1277"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1277"/>
    <dbReference type="eggNOG" id="COG2893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123235_1_1_9"/>
    <dbReference type="OMA" id="IVHNAQN"/>
    <dbReference type="OrthoDB" id="2008912at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00006">
        <property type="entry name" value="PTS_IIA_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033887">
        <property type="entry name" value="PTS_IIA_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-4" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="14736" checksum="EE0A2AD979709E2A" modified="2004-10-11" version="1">MIGVLIVTHGRFGEALLESAGMLVSDLDQVRTVSFLPGQGVEDLEAAVGDALRELGESEGILALVDIPGGTPARVLGGVALQRADLELVSGVNLAMLVEVLFARLSATLPELVGMAVRGGWDGIRDLGAAVRHEATVGGDAPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q53</accession>
    <name>Q67Q53_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Soprulation-specific penicillin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1205</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transpeptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195336.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1205"/>
    <dbReference type="EnsemblBacteria" id="BAD40190">
        <property type="protein sequence ID" value="BAD40190"/>
        <property type="gene ID" value="STH1205"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1205"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_0_9"/>
    <dbReference type="OMA" id="SCDTYYY"/>
    <dbReference type="OrthoDB" id="213680at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="5">
        <location>
            <begin position="578"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="5">
        <location>
            <begin position="642"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="707" mass="77011" checksum="8FB8777EC11D7888" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE5</accession>
    <name>Q67QE5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1113</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0702 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1113"/>
    <dbReference type="EnsemblBacteria" id="BAD40098">
        <property type="protein sequence ID" value="BAD40098"/>
        <property type="gene ID" value="STH1113"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1113"/>
    <dbReference type="eggNOG" id="COG2323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1179724_0_0_9"/>
    <dbReference type="OMA" id="QMTSFDL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007353">
        <property type="entry name" value="DUF421"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023090">
        <property type="entry name" value="UPF0702_alpha/beta_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04239">
        <property type="entry name" value="DUF421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="26381" checksum="27648E079D1ACA16" modified="2004-10-11" version="1">MWILAQIFTSDWAKELIQHVPVLLMRTVLTFILVMIVVRWTGKRSIANLAPFDLAMMIMIGEVAAIPIGELDVDFLHGLIPVALLGGLHVALTTVNLHWKSFERWTEGFPTLLVKDGRVLRRNLLKERVSMADLMTALRHKEVEDVSEVKEAWMEQAGGISVILKREAGPATPRDVELVVERVLARRLPGVVQEAVERAVRDAIAAAGKPSAEAHARPRQPDGRRWYREGDDVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG1</accession>
    <name>Q67QG1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195229.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1097"/>
    <dbReference type="EnsemblBacteria" id="BAD40082">
        <property type="protein sequence ID" value="BAD40082"/>
        <property type="gene ID" value="STH1097"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1097"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_3_2_9"/>
    <dbReference type="OMA" id="FLAEGPQ"/>
    <dbReference type="OrthoDB" id="1697536at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="35"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="28029" checksum="21F8403C9EF98FB9" modified="2004-10-11" version="1">MTRAETPLGPKHALVRQARELARERRAREESGLAHMEGVRLVEEALAAGVEVAYLLYTPELAERPRGAELLRTAAARGVPLHPVTPEALERAADTRTPQGVVGVFRPLSRTLDDLGPGLVLVLDGLQDPGNLGTAIRSLEAMGGGGAVVAGGVDPYNPKVVRGAMGSLFRLPVVKRPIGDALAGLRAAGRRIYLAEAGGALAPWAVDLARGAAVVVGSEAAGPSPEARALADGVVSIPMAGPTESLNAGVAASLLAYEAMRQQGAAAPPALSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG5</accession>
    <name>RL20_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplT</name>
        <name type="ordered locus">STH1093</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195225.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QG5"/>
    <dbReference type="SMR" id="Q67QG5"/>
    <dbReference type="STRING" id="292459.STH1093"/>
    <dbReference type="EnsemblBacteria" id="BAD40078">
        <property type="protein sequence ID" value="BAD40078"/>
        <property type="gene ID" value="STH1093"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1093"/>
    <dbReference type="eggNOG" id="COG0292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123265_0_1_9"/>
    <dbReference type="OMA" id="GRRKNVW"/>
    <dbReference type="OrthoDB" id="1932144at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000177244" description="50S ribosomal protein L20">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <sequence length="117" mass="13318" checksum="A18AE9534A83AF62" modified="2004-10-11" version="1">MARTKPGKTTRARHKKILKLAKGYYGARSKHFRPANETVMKALFYARRDRRQRKRNFRRLWIARINAAARMNGLTYSQFINGLNKAGVQLNRKVLADIAVNDAAGFSALVEKAKASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH4</accession>
    <name>Q67QH4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec-independent protein translocase protein TatC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tatC</name>
        <name evidence="6" type="ordered locus">STH1084</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with TatA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195216.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1084"/>
    <dbReference type="EnsemblBacteria" id="BAD40069">
        <property type="protein sequence ID" value="BAD40069"/>
        <property type="gene ID" value="STH1084"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1084"/>
    <dbReference type="eggNOG" id="COG0805">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031942_3_1_9"/>
    <dbReference type="OMA" id="RMLNYSA"/>
    <dbReference type="OrthoDB" id="1848013at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019820">
        <property type="entry name" value="Sec-indep_translocase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="PTHR30371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01218">
        <property type="entry name" value="TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="26595" checksum="1E9ADC8C0B6B2552" modified="2004-10-11" version="1">MQDKELTLVEHLTELRERIIKSLIAVAIGFFAAVFLSQPVFQHIMEQAKAEGVQLIQYNFADTFITQIKLALILGLVLAFPVVLYQVIAFILPALKPEEKRLLFIGLPFATGLFLAGWAFGYFIVVPITKEFFKSMAQASLVEVAVTPSNYLSFILSICNPLGIAFELPLVVLILARIGLVTHQFLGRVRKYAFLALMILAAVLSPPDVISMMIFLVPLYGLYEFSIILARFAQPKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL9</accession>
    <name>Q67QL9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyrimidine-nucleoside phosphorylase</fullName>
            <ecNumber evidence="7">2.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH1039</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes phosphorolysis of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and ribose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2'-deoxyuridine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:22824"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + thymidine = 2-deoxy-alpha-D-ribose 1-phosphate + thymine</text>
            <dbReference type="Rhea" id="RHEA:16037"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:17821"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>phosphate + uridine = alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:24388"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.2" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195171.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1039"/>
    <dbReference type="EnsemblBacteria" id="BAD40024">
        <property type="protein sequence ID" value="BAD40024"/>
        <property type="gene ID" value="STH1039"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1039"/>
    <dbReference type="eggNOG" id="COG0213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025040_0_1_9"/>
    <dbReference type="OMA" id="DVWRRMI"/>
    <dbReference type="OrthoDB" id="1724909at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016154">
        <property type="term" value="F:pyrimidine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006213">
        <property type="term" value="P:pyrimidine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018090">
        <property type="entry name" value="Pyrmidine_PPas_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="PTHR10515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="SSF54680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02644">
        <property type="entry name" value="Y_phosphoryl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PYNP_C" evidence="11">
        <location>
            <begin position="345"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="442" mass="47050" checksum="2671AFFE3DA84B2C" modified="2004-10-11" version="1">MRAYDIIYRKRMGQELTTDEIRFLVRGYVSGEVTDYQMAAWAMAVCIQGMTPRETADLTMAMAESGDQIDLSGIRGRKVDKHSTGGVGDKVSLVLVPLVAACGAPVAKMSGRGLGHTGGTIDKLESIPGFRVELSPQEFIDQVNRVGCAVAGQSGNLVPADKKLYALRDVTATVDSIPLIASSIMSKKIAGGADAVVLDVKAGAGAFMKDLDQAFRLAEMMVAIGREVGREVAAVVSNMDQPLGYAVGNALEVAEAIETLRGFGPKDLEEICLQLGSQMLRLAGVAPDGESARKMLLRALRAGHGLNKLIEMVEAQGGDPSYIRQPIKLPRAKRIVPVAAPRSGYVQAIDALEVGVAAMLLGAGRETKESAIDLGVGVVLRKKVGDPVEAGEPLAELHVNSERRLDEARERLAGAYRIGGERLQEPPLVYGLVTSHGTERWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS1</accession>
    <name>Q67QS1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH987</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH987"/>
    <dbReference type="EnsemblBacteria" id="BAD39972">
        <property type="protein sequence ID" value="BAD39972"/>
        <property type="gene ID" value="STH987"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH987"/>
    <dbReference type="eggNOG" id="COG3279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_14_1_9"/>
    <dbReference type="OMA" id="IQAFEEH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007492">
        <property type="entry name" value="LytTR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04397">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00850">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50930">
        <property type="entry name" value="HTH_LYTTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HTH LytTR-type" evidence="8">
        <location>
            <begin position="149"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="29250" checksum="59F054207B3C47E7" modified="2004-10-11" version="1">MLRALIVDDEYPARMELRYQLSQFPDVEVIGEATNAREAMALINALDYDVIFLDVQMPGMTGIELAKSLKGRPVMPKVVFVTAYENYAVSAFEIPATDYLLKPIEAERLGETIQRLREAKEAAAGEARPEASEGAERPAGASKPQLTFLMCEKDDKQIPLPLNEIVYIFSEGYNVYVQTYSERYLTRHTLQELTERLPSSQFFRSHRSYLVNIYQVKEISPYFNGAYILKLKDKEHSEVIVSRSNVKRMKQLFSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS9</accession>
    <name>Q67QS9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cu_amine_oxidN1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH979"/>
    <dbReference type="EnsemblBacteria" id="BAD39964">
        <property type="protein sequence ID" value="BAD39964"/>
        <property type="gene ID" value="STH979"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH979"/>
    <dbReference type="HOGENOM" id="CLU_1502739_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="84"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="18220" checksum="DB043DB8EFD05F10" modified="2004-10-11" version="1">MESDAGGAGGSRVPTSRKGGLRTAPVLVPALLAFGAAALCGGGLIASVAPAAGCPPLAGAQPVAVTARAADGDAHGVTGYLFRNGEALIPVRGLVERTGRIYWDESGQTVTLLGPRDVLSVHVPGGRAETRIAVLNGEVIAAHAVRCEEQVYLPADLVATVLHLEVAVPSDDRGERMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV1</accession>
    <name>Q67QV1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195089.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH957"/>
    <dbReference type="EnsemblBacteria" id="BAD39942">
        <property type="protein sequence ID" value="BAD39942"/>
        <property type="gene ID" value="STH957"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH957"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_2_2_9"/>
    <dbReference type="OMA" id="DTVSFHN"/>
    <dbReference type="OrthoDB" id="1818437at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="21867" checksum="57ECAE9F9F4A730D" modified="2004-10-11" version="1">MVRIGIIIGSTRPGRNGEAVARWVYEHARKRTDAEFELVDLKDFNLPVYDEPVPPSYGKNYQHEHTRRWSAKIDSFDGFIFVTPEYNHATSGALKNAIDYLYLEWNNKAAGLVGYGSLGGARAIENLRAILSEVQIAHVRQQVSFSLFTDFEDFKVFKPNEEVHLPALNVMLDQLIAWSAALKRVRQEAAAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ2</accession>
    <name>Q67QZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nitrate reductase component, ferredoxin-type protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">napG</name>
        <name evidence="6" type="ordered locus">STH916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195048.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH916"/>
    <dbReference type="EnsemblBacteria" id="BAD39901">
        <property type="protein sequence ID" value="BAD39901"/>
        <property type="gene ID" value="STH916"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH916"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077329_0_1_9"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="62"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="18427" checksum="143BF92DC057DE42" modified="2004-10-11" version="1">MRPHRCWRPAAGGWLGRRLLRPPGALPEPFFLGLCLRCGQCAQACPRSAIRIARWEHGLSTGTPYIAARETACDLCGGQAPACAAVCPTQALHLEPGEPVRMGTAVIDATRCLAHMGDICRSCFNACPLRGRAIVLEGALRPVVDPALCTGCGLCEEHCLVEPAAIHVEPLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R16</accession>
    <name>Q67R16_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH892"/>
    <dbReference type="EnsemblBacteria" id="BAD39877">
        <property type="protein sequence ID" value="BAD39877"/>
        <property type="gene ID" value="STH892"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH892"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092441_0_0_9"/>
    <dbReference type="OMA" id="SHVFAAR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="41"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20158" checksum="1FBC031107A78270" modified="2004-10-11" version="1">MTGEVLARAEAELGRPVVLTWRYPILPDEMAMVVASTRGQQRLHDVTLFIFDPAGRLALIRKHHYPPGIWRAPGGGVKPGEEFAAGAAREGWEETGLAVRVTRYLLRVHVTFTCGGQEQPWTTHVVLAEGEGDPATRDPREIGGVKWGSMEELCGPIADAMLATGRGLFAYRVALHRQVAQLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R48</accession>
    <name>Q67R48_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH860"/>
    <dbReference type="EnsemblBacteria" id="BAD39845">
        <property type="protein sequence ID" value="BAD39845"/>
        <property type="gene ID" value="STH860"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH860"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="39339" checksum="E0DAAA9D6AF247B4" modified="2004-10-11" version="1">MRLRLVTVPDRLWADRSIPEGAKLVWCYVSALCHLRSEFTYKELREGAGISLPSLHKYLSVLSRAGWLNCARISLRVVRCEITGPMGGPRLVLPTDILFERRLPRGACWTWGLIGRMGGRFEYTELRKATGYSQDTLSKHVRALIAQRWLVGGPHRKARRVIYNVRGANPRAIQRAQELQELERGLQVARSTPGYSQGQYILARLIREMYPGVEVLENAEITGLDNVETRGRLQVDVYMPDQKLAIEFQGHQHAGPTERYPSVEEFRARRRRDLIKRGLCLEKGIELISLWPQDLSCAGIARALGHRLHFAGAREDRWHVARFLEQRAEWYRKAAARSQCSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R65</accession>
    <name>Q67R65_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH843</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194976.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH843"/>
    <dbReference type="EnsemblBacteria" id="BAD39828">
        <property type="protein sequence ID" value="BAD39828"/>
        <property type="gene ID" value="STH843"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH843"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_339452_0_0_9"/>
    <dbReference type="OMA" id="VYIEHES"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007891">
        <property type="entry name" value="CHASE3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05227">
        <property type="entry name" value="CHASE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="212"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="616"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="252"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="462"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="837" mass="92725" checksum="FC58B09F62C84668" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN3</accession>
    <name>Q67RN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH675"/>
    <dbReference type="EnsemblBacteria" id="BAD39660">
        <property type="protein sequence ID" value="BAD39660"/>
        <property type="gene ID" value="STH675"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH675"/>
    <dbReference type="HOGENOM" id="CLU_3141510_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="49" mass="5187" checksum="11DEF5A89B681188" modified="2004-10-11" version="1">MGIRIAGNLLTADGMDCTRPSPLAPWRLTDDGHGLPGNGLYKRSATVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN6</accession>
    <name>Q67RN6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39657">
        <property type="protein sequence ID" value="BAD39657"/>
        <property type="gene ID" value="STH672"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH672"/>
    <dbReference type="HOGENOM" id="CLU_2248739_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="11509" checksum="651F63EDA4636BDC" modified="2004-10-11" version="1">MRPHDLPACGWRPASRCACSPSPAFDCGWPYGWPLRSAGCGDRARKTNGPHSSRCGSGRQPYPQSPGLPDPVRCSPPGRMRQYNHSRVIIRLFGRPSFLPGFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP6</accession>
    <name>Q67RP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39647">
        <property type="protein sequence ID" value="BAD39647"/>
        <property type="gene ID" value="STH662"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH662"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="285"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="541" mass="60931" checksum="6271DF00C0721869" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX9</accession>
    <name>Q67RX9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194713.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH579"/>
    <dbReference type="EnsemblBacteria" id="BAD39564">
        <property type="protein sequence ID" value="BAD39564"/>
        <property type="gene ID" value="STH579"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH579"/>
    <dbReference type="eggNOG" id="COG1541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_780634_0_0_9"/>
    <dbReference type="OMA" id="GSPFWLE"/>
    <dbReference type="OrthoDB" id="839891at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="42333" checksum="70C4E2A609C30FA1" modified="2004-10-11" version="1">MTRAELDAHVRDIVRWHFSPDTGSPYWLRKAADLGFDPVAEVRGFDDLARFPAVAGEWRSVPVGDLIPRGCSEPFDVWETGGTTGPPVRIVDAQERTRGIQRVDRMLDEHGFPRREARTRPGGPTGAIAPAHGGTDTTEGRAHTGGGDAYPAWLHLGPTGPHLVGTNVARLARLRGFLYHTVDLDPRWVKRLYREGRADEARRYTAHLVEQALAVLRTQPVRVLSTTPPLLQALCEHPEAYAALAGRVEGIIWFGTSLSAEGLRLLEEELLPEARFVGWYGNTLMGIACQRPRREGDTHRCIFSPPGPAAVVRVVDPQDPSRRVAPGEAGQVRISLLTRELFLPWHLERDRAVRVPWPGSELWDELAEVAPLDGGGARVEGVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S27</accession>
    <name>YBEY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ybeY</name>
        <name type="ordered locus">STH531</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194665.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S27"/>
    <dbReference type="SMR" id="Q67S27"/>
    <dbReference type="STRING" id="292459.STH531"/>
    <dbReference type="EnsemblBacteria" id="BAD39516">
        <property type="protein sequence ID" value="BAD39516"/>
        <property type="gene ID" value="STH531"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH531"/>
    <dbReference type="eggNOG" id="COG0319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106710_3_0_9"/>
    <dbReference type="OMA" id="RMRIHPL"/>
    <dbReference type="OrthoDB" id="1830156at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="PTHR46986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000102548" description="Endoribonuclease YbeY">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <sequence length="156" mass="17275" checksum="5A43BFA123C5D8FD" modified="2004-10-11" version="1">MEIWVNNDQEIVEFTEEHEELIRRVAERALELAGAGLGSNVSVSVTLVDDETITDLNRDHRGLASPTDVLSFSQLEGEDMGDLPEGEPMPLGDIVISLERCVSQAAEYGHSFERELGFLTAHGMLHLMGWDHQTPEDEARMMAKTEEILAGLGLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S33</accession>
    <name>Q67S33_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="7">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lysS1</name>
        <name evidence="7" type="synonym">lysS</name>
        <name evidence="10" type="ordered locus">STH525</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7 8">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194659.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH525"/>
    <dbReference type="EnsemblBacteria" id="BAD39510">
        <property type="protein sequence ID" value="BAD39510"/>
        <property type="gene ID" value="STH525"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH525"/>
    <dbReference type="eggNOG" id="COG1190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_9"/>
    <dbReference type="OMA" id="EIFGEKC"/>
    <dbReference type="OrthoDB" id="63621at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="174"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="495" mass="55531" checksum="3DBEC5296BEF5FB1" modified="2004-10-11" version="1">MLSREEQLREARRAKMRTLREAGVDPFGTRFGDRRLMGDLRRQYGEASAEELDAARIPARVAGRVVTVRSHGKIGFAHLQDGSGRLQIYLRLPELSERERLVWEHLDLGDIVGAEGVLMRTRTGELTVKVESLTMLTKALRPLPDKHAGLRDVELRYRERHLDLLVNPESRAVFEARSRIVASLRRTLDELGFIEVETPVLVPLAGGAAARPFLTYHNALDRQLSLRIATELHLKRLVVGGMERVYEIGRVFRNEGLSTRHNPEFTSLEAYQAYADYRDMMDLTERLVQNAARAVNGSLRAGEIDLTGPFRRITMAEAVKEATGIDVMALGSSGDERRDAEAVRAAAEAAGYEVEAATWGGLLAELFERYAEDRLVQPTFVLGHPVETSPLARRDPRDPRLTERFELYVQGREIANAFSELNDPEDQRRRFEAQLAQRQAGDEEAHPMDEDFLAALEVGLPPTGGLGIGVDRLVMLLTGAPSIRDVILFPTMRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S62</accession>
    <name>Q67S62_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MutT/nudix family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH496</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH496"/>
    <dbReference type="EnsemblBacteria" id="BAD39481">
        <property type="protein sequence ID" value="BAD39481"/>
        <property type="gene ID" value="STH496"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH496"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101758_3_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="20180" checksum="CF5DD8A0EB698606" modified="2004-10-11" version="1">MVHLTDPAYDRSPTADPKTHNPKELFREMTAVTRDFTVATFVVHEGKVLLLWHRKLGMWLPPGGHIEPNELPDEAAVREVREEAGLEVRLISPPALPPIPGPRQLARPEGVQLEQIAPGHEHIDLIYFARPADPGAVRLTANHEVDRVGWYGPEDLDRIPLTDEVRAWVRKALAAAAERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE4</accession>
    <name>Q67SE4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 14">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">STH414</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="12 14">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 14"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH414"/>
    <dbReference type="EnsemblBacteria" id="BAD39399">
        <property type="protein sequence ID" value="BAD39399"/>
        <property type="gene ID" value="STH414"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH414"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_9"/>
    <dbReference type="OMA" id="DAKYGEW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 12 14" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10 14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="16">
        <location>
            <begin position="9"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="15">
        <location>
            <begin position="348"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="185"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="320"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD39399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="470" mass="49652" checksum="C8A35200D8ED1077" modified="2004-10-11" version="1">MGSIKTGTDVVVIGAGPGGYVAAQRASQLGLDVTLIEREELGGTCLNHGCIPSKALISVGDLLYKVNNAAERGLVVKGSVEVDFAKTQEWKETKVIKRLTSGVASLMKAGQVEVVKGTARFTDPHSLEVELNDGGTAAYTFKHAIIATGSTAVNPSFFPLDGENVVDARGALAFREIPPRFVVVGGGYIGVELGIAYAKLGSKVTIVEATGQLLPGTDPDLVNVLMRRLRRLGVTVMLNARASGGLQNGKVKVQDGEGKVHEIEADKVLVSVGRVPYTEGLHLDKAGVRVDEKGFIPVDEQMRTNVPHIYAIGDVCSPVMLAHKASAQGRVAAEAIAGRPSAADWQTVPAVIFTDPEIAYVGLTEAQAREKGYDPVVSRYNFAAVGRALTMGESDGMVKLVGDRQSGLLLGAQMVGPEVSELIGEIALAIEMGAQMEDVALTPHYHPTLSEGILEAALSWMHSIEKAGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE7</accession>
    <name>Q67SE7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="3 8">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pdhA</name>
        <name evidence="10" type="ordered locus">STH411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="3 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194545.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH411"/>
    <dbReference type="EnsemblBacteria" id="BAD39396">
        <property type="protein sequence ID" value="BAD39396"/>
        <property type="gene ID" value="STH411"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH411"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_9"/>
    <dbReference type="OMA" id="DWHTGYD"/>
    <dbReference type="OrthoDB" id="1248201at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017596">
        <property type="entry name" value="PdhA/BkdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF41">
        <property type="entry name" value="PTHR11516:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03181">
        <property type="entry name" value="PDH_E1_alph_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="E1_dh" evidence="9">
        <location>
            <begin position="43"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="368" mass="40904" checksum="9E62608ED766EE4A" modified="2004-10-11" version="1">MELQTALPRGDQLFPVRRILQADGTLLEPVPAFLSVDQLKDVYRKMVYLRVFDQRCLNLQRQGRMGTFAPFSGQEASQVGSAYLLRPDRDWIFPTYRDHGAMHVMGVPLVNILRYFMGDEQGSHAPQGVNAFPISIPIATQLLHAVGAAWAGRIKGEDTVAVGYAGDGGTSPGDFHEALNFAAVFNVPVIFFIQNNRYAISTPNSRQFKTPTIAQRALGYDIAGVRVDGQDVLAVLAVMHEAIERARSGGGPTLVESVTFRYGPHTTSDDPKRYRSQEELEEWQARDPIERLRLYLVSQGQWSDSDDEALWTAAREQVAAAVAEAEAMPRPSVDDLFDYLYAEPTPNLVRQKEYLKAYLAKKEGGARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG4</accession>
    <name>Q67SG4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tad domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194528.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH394"/>
    <dbReference type="EnsemblBacteria" id="BAD39379">
        <property type="protein sequence ID" value="BAD39379"/>
        <property type="gene ID" value="STH394"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH394"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102344_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028087">
        <property type="entry name" value="Tad_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13400">
        <property type="entry name" value="Tad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tad" evidence="1">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="27625" checksum="16DCC2CAD9857A7C" modified="2004-10-11" version="1">MNRFRTEQRGSTGALFVFIWAVAVLAIGFALDVGRVFVLREQLRTAEEAAALAGVRQARYVVEAWFRREENRPVTVCETVPPPVPGGEPAVECWLVDNWVEISPAVIQGPEAEVWPDAHRIWAEQCVGYNVRCARQYHAAECWIEPRAGWGEVRAAAVEAFQMNARWGGLARTVGPVAVEVATDTPLRPRQLRVAVTAVLEMDALLLPLVGVDTLTVVTPGGATAAELVRREWAGQYTWIGGVRVASPCLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI4</accession>
    <name>Q67SI4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rod shape-determining protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mreD</name>
        <name evidence="9" type="ordered locus">STH374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MreD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194508.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH374"/>
    <dbReference type="EnsemblBacteria" id="BAD39359">
        <property type="protein sequence ID" value="BAD39359"/>
        <property type="gene ID" value="STH374"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH374"/>
    <dbReference type="eggNOG" id="COG2891">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132534_2_0_9"/>
    <dbReference type="OMA" id="LNGPWEA"/>
    <dbReference type="OrthoDB" id="1487171at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017225">
        <property type="entry name" value="Cell_shape_determin_MreD_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007227">
        <property type="entry name" value="Cell_shape_determining_MreD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04093">
        <property type="entry name" value="MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037497">
        <property type="entry name" value="MreD_Clostridium/Treponema_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03426">
        <property type="entry name" value="shape_MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="171" mass="18204" checksum="65B4FB5770F71186" modified="2004-10-11" version="1">MRYWVLTAITLVAYLVESVLGPFLAIGGVAPNVVFVVAVSCGLLFGWQVGLGVGFGGGLLIDLTIGQLIGSHALAGSLVGFIAGVVEERVFKDNLLLPLIGGLVGSVVGQAVVLASLLLFGRHVPLAELSGKLLRSVAYDTVLCALVYWRIFRMYGFIRPDPRGVIVLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ0</accession>
    <name>Q67SJ0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPOR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH368"/>
    <dbReference type="EnsemblBacteria" id="BAD39353">
        <property type="protein sequence ID" value="BAD39353"/>
        <property type="gene ID" value="STH368"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH368"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979788_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036680">
        <property type="entry name" value="SPOR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110997">
        <property type="entry name" value="SSF110997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51724">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="SPOR" evidence="2">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="29665" checksum="B67ACBCA8659D21A" modified="2004-10-11" version="1">MHRFGRSVQKRANDRWGGVFILAVVLAVVGAWQLGGVLGNLLGEEKPAAMEPVPGLEEATGISGPAGSPSQVAIVPRPFQVHFVQVGAFRSEGAARNLVSELAGQQVVAAMTPRNAMGLVKVYVGPFMSGEEAGEVLDVMRETGVAPNAFTVALDVDYAPEAVMAMTGSANPDLQTGLDALNNYLYEAGAWFARRAAGEPADGGMLVALGQEVRQAAARLAAAEENPAITRFLGLADMASVNAADIEVAASAAPGSEEFQRAMNGYVSLLEQYHNFYAADAASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ3</accession>
    <name>Q67SJ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH365"/>
    <dbReference type="EnsemblBacteria" id="BAD39350">
        <property type="protein sequence ID" value="BAD39350"/>
        <property type="gene ID" value="STH365"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH365"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_2_9"/>
    <dbReference type="OMA" id="LVEINWA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="19992" checksum="B7586A059158FC04" modified="2004-10-11" version="1">MPGLPRVGLRPVEQADLALLSRWDEDPVIGALMGRRFVGQSPAQWLEDVRHSRTSRVWMIEWEGRTVGEVELAQVNRRAGTAEVRVCVGEKALWGRGIGTAAMSALLNEAFVVMGLETVYLRVFTTNLRAIGLYERLGFRREGLLAPSSRRADPAPVLLMNLSRSRWLARKRREPALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST0</accession>
    <name>Q67ST0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH278"/>
    <dbReference type="EnsemblBacteria" id="BAD39263">
        <property type="protein sequence ID" value="BAD39263"/>
        <property type="gene ID" value="STH278"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH278"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055286_0_1_9"/>
    <dbReference type="OMA" id="CPMTFQE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="35872" checksum="43A042B8913D959E" modified="2004-10-11" version="1">MLRPEVLILSNRHDYASDYICSQLWEQGIPYLRLNTDDMPEMELCLYPTEPRLEGVVSGLEFCIDSALRSVYFRCPVFLREMPGIALPAHDQMVRSQWASFVRNLTVFQEALWVNHPGKTYLSENKALQLLEARKAGLTVPRSIVTNSTKKVTTEFAGESRVMLKSLDTLLVRQGDVEAFAYAVPWNLSELAKETMSAAPATVQPYFEPKVDWRVTVVGKKLFAVKILKDEKGVSGDWRLEKDRVRFVPSNLPAPVSSACLRLTKQLGLIFGAIDLVEVGGEFVFLEINPTGEWAWLVEQAGLPIDREIAKILRQGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST1</accession>
    <name>Q67ST1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0236 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH277"/>
    <dbReference type="EnsemblBacteria" id="BAD39262">
        <property type="protein sequence ID" value="BAD39262"/>
        <property type="gene ID" value="STH277"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH277"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040782_3_1_9"/>
    <dbReference type="OMA" id="FNISHAQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009620">
        <property type="entry name" value="UPF0236"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06782">
        <property type="entry name" value="UPF0236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="485" mass="55410" checksum="490FB73F412F35E0" modified="2004-10-11" version="1">MAPSKSVVAGKTPTQGGSSLVMVEIRADNGFSFEELESGVWQQVVECFREVLVEALSRVDTLLYENRDAQRYVFKEMRSRTLMTKFGPITFKRRYYWDQEEKRFVFLLDEVLQIAKRQRVSESVRADAVEAAVTAGSFRGAAAELGRRDCQVYVSHEAIRQWSIETGMALAAAQKQRQVTEGGTRKVRFLYIEADGFWPGRQRGKKAEVRLFVIHEGWVERTPAGTEYRLVNRRDFIPDSGRDSWEQLSEFLEREYDLSDTWVIINGDRARWIREGVTWFPKALYQIDRFHLKQELNHVLRHQPRLLERANAALETNDAGGLLAVLEEARTAETDSKRRGEIRRLVADLRTMPESIRDYRVRLQERGVSVEGLRGLGAAEGAVERYSARLRKVGRSWSERGLKAMMHVLAAYFHGTLRGAVEMVERQLGLESLAAVREKVRHRVVETVGRGIEGVRHGRMPILYAGRNASGGYSRMLRAAAGFTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV9</accession>
    <name>ENO_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">STH249</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194384.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SV9"/>
    <dbReference type="SMR" id="Q67SV9"/>
    <dbReference type="STRING" id="292459.STH249"/>
    <dbReference type="PRIDE" id="Q67SV9"/>
    <dbReference type="EnsemblBacteria" id="BAD39234">
        <property type="protein sequence ID" value="BAD39234"/>
        <property type="gene ID" value="STH249"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH249"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_9"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000133989" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="365"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="430" mass="46030" checksum="EC9EAD1F599DADD9" modified="2004-10-11" version="1">MTTIVNVHAREVLDSRGNPTVEVEVILASGTTGRAIVPSGASTGQFEAVELRDGDKQRYLGKGVLTAVRNVNEVIAEAVVGMDATEQVEVDQAMLALDGTPNKSKLGANAILGVSLATARAAAAELGIPLYRHVGGLTARTLPVPMMNIINGGKHADNNVDMQEFMIFPAGAPSFAESLRMGTEVFHALKSVLKSKGYNTAVGDEGGFAPDLKSNEEAILVILEAIEKAGYKPGQDVFLCMDVASSELFQDGKYVLAGEGNKVLSTEQLIDLWDSWTRQYPIVSIEDGVAEDEWEAWVELTRRIGDRVQLVGDDFFVTNTERLARGIGMNAANAILVKVNQIGTLTETLEAVALAHRNGYSSVMSHRSGETEDTTIADLAVALNCGQIKTGAPSRTDRVAKYNQLLRIEEELGDAAIFAGTSVIRGRKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T04</accession>
    <name>Q67T04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage V sporulation protein AE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194339.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH204"/>
    <dbReference type="EnsemblBacteria" id="BAD39189">
        <property type="protein sequence ID" value="BAD39189"/>
        <property type="gene ID" value="STH204"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH204"/>
    <dbReference type="eggNOG" id="ENOG50315MJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112786_1_0_9"/>
    <dbReference type="OMA" id="ITGIFEV"/>
    <dbReference type="OrthoDB" id="1965283at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014204">
        <property type="entry name" value="Spore_V_AE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005562">
        <property type="entry name" value="SpoVA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38450">
        <property type="entry name" value="PTHR38450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03862">
        <property type="entry name" value="SpoVAC_SpoVAEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02839">
        <property type="entry name" value="spore_V_AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="11714" checksum="40E041F52545D223" modified="2004-10-11" version="1">MMYLKAFLVGGLICLIAQVILDNTKLSPGHVLSGLTVAGGVLGGLGLYDKLVEFAGAGASVPIASFGNALVKGALQELDQNGLVGVLTGMFEVTSTGIAAAIVFAFLTAVTFNPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T10</accession>
    <name>Q67T10_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine synthetase I alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH198</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194333.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH198"/>
    <dbReference type="EnsemblBacteria" id="BAD39183">
        <property type="protein sequence ID" value="BAD39183"/>
        <property type="gene ID" value="STH198"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH198"/>
    <dbReference type="eggNOG" id="COG0174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_2_9"/>
    <dbReference type="OMA" id="VFPWESK"/>
    <dbReference type="OrthoDB" id="416474at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="5">
        <location>
            <begin position="102"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="3">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="471" mass="52500" checksum="76B344C6AB6B783B" modified="2004-10-11" version="1">MAKVDRVLEMIAEKDIRLLDLKFVDVLGMLHHLTVPVEVVDAEQLSQGFFFDGSSIRGFRGIAESDMILRPDPATVVCDPFVEGPALSLMADVLEPGLREYGRDPRGVARRAEEYLRQTGIADTSYWGVEIEFFVFDNVRFQTGPDASFYYVDSAEAHWNSGRVPNGGYKVKHQQGYLRALPIDEQTDLRTAMTRQMQALGLVVERHHHEVASAGQNEINFRFSTLTDTADKAMLYKYAVKNVARAHGKTATFMPKPLYRNSGNGMHCNQSLAKDGVNLFYDPNGYANLSQIALWYIGGLLTHADALLAITNPTTNSYKRLVPGYEAPTNLAFSRANRSAAIRIPVSLSPKTTRIEFRTPDPTANPYLALAAQLMAGLDGIRRRIDPTAAGFGPVDIDIYHHAGSAAVQLKHVPSSLRQAVEALKADHEFLLEGDVFSEDLIETFIALKEDEIRNLETHPHPVEFEMYFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T17</accession>
    <name>Q67T17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Redox-sensing transcriptional repressor Rex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rex</name>
        <name evidence="9" type="ordered locus">STH191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transcriptional regulatory Rex family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194326.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH191"/>
    <dbReference type="EnsemblBacteria" id="BAD39176">
        <property type="protein sequence ID" value="BAD39176"/>
        <property type="gene ID" value="STH191"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH191"/>
    <dbReference type="eggNOG" id="COG2344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061534_0_1_9"/>
    <dbReference type="OMA" id="HEQRKAG"/>
    <dbReference type="OrthoDB" id="1872374at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01131">
        <property type="entry name" value="Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009718">
        <property type="entry name" value="Rex_DNA-bd_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022876">
        <property type="entry name" value="Tscrpt_rep_Rex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35786">
        <property type="entry name" value="PTHR35786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06971">
        <property type="entry name" value="Put_DNA-bind_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CoA_binding" evidence="8">
        <location>
            <begin position="79"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="7">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="90"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="23092" checksum="47984DA334E791AA" modified="2004-10-11" version="1">MRRTNIPDAAIRRLPVYLRVVSDLAAADVPIVSSAELAEKTGLSPEQIRKDLAYFGAFGTRGVGYDTAMLSRRIARILGLHRGVKAALVGFGHLGYALARYSLTQHKDIVIAAIFDVDPEKVGQEILGVRVQHVSEMVETCRQQGVKIGIITVPASSAQQTAELLKAGGVEAILNFAPAKLNVDGVFVQNIDLTIELQSLAYYTSASEDDTAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T87</accession>
    <name>Q67T87_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translational regulator CsrA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">csrA</name>
        <name evidence="6" type="ordered locus">STH121</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">A translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Usually binds in the 5'-UTR at or near the Shine-Dalgarno sequence preventing ribosome-binding, thus repressing translation. Its main target seems to be the major flagellin gene, while its function is anatagonized by FliW.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; the beta-strands of each monomer intercalate to form a hydrophobic core, while the alpha-helices form wings that extend away from the core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CsrA/RsmA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194256.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH121"/>
    <dbReference type="EnsemblBacteria" id="BAD39106">
        <property type="protein sequence ID" value="BAD39106"/>
        <property type="gene ID" value="STH121"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH121"/>
    <dbReference type="eggNOG" id="COG1551">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164837_0_2_9"/>
    <dbReference type="OMA" id="RDESIMI"/>
    <dbReference type="OrthoDB" id="2032250at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902208">
        <property type="term" value="P:regulation of bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00167">
        <property type="entry name" value="CsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003751">
        <property type="entry name" value="CsrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036107">
        <property type="entry name" value="CsrA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34984">
        <property type="entry name" value="PTHR34984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02599">
        <property type="entry name" value="CsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117130">
        <property type="entry name" value="SSF117130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00202">
        <property type="entry name" value="csrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="10000" checksum="02593080A29237CE" modified="2004-10-11" version="1">MLVLTRRVEESILIGDDIEIKILQVRGAGAQAVVRLGIVAPKSVTILRKEIYDEVVDANRQAAQAAAPLPPDLLRALGGAAPGPDPEEGPGGEQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD9</accession>
    <name>Q67TD9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Penicillin amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH69</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194204.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH69"/>
    <dbReference type="MEROPS" id="S45.003"/>
    <dbReference type="EnsemblBacteria" id="BAD39054">
        <property type="protein sequence ID" value="BAD39054"/>
        <property type="gene ID" value="STH69"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH69"/>
    <dbReference type="eggNOG" id="COG2366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_0_1_9"/>
    <dbReference type="OMA" id="EMDVRRH"/>
    <dbReference type="OrthoDB" id="186419at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043147">
        <property type="entry name" value="Penicillin_amidase_A-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023343">
        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043146">
        <property type="entry name" value="Penicillin_amidase_N_B-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218">
        <property type="entry name" value="PTHR34218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001227">
        <property type="entry name" value="Pen_acylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001227-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001227-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="822" mass="90522" checksum="BCB0072653F46C47" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH9</accession>
    <name>Q67TH9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma-W factor RsiW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH29</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily. Anti-sigma-W factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194164.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH29"/>
    <dbReference type="EnsemblBacteria" id="BAD39014">
        <property type="protein sequence ID" value="BAD39014"/>
        <property type="gene ID" value="STH29"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH29"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1057390_0_0_9"/>
    <dbReference type="OrthoDB" id="1494577at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="zf-HC2" evidence="4">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28173" checksum="4F40FC3F9958FCC1" modified="2004-10-11" version="1">MQCEQIQSELTAYLYGTLSPQERAAIEAHLMQCEACAEEASAMKDIGDKLSQGLKDWVNQGVCPPEVAERIQLSLKGESRRSAWRRAAAAFGSVAAVAAVFLFALSTQPQFAQHVASVPFLGALAAQLLEPDLEIHLNPQQRMTAALFRPSRSVTLKVTESGDGLTLTVTSVATGESATRIAYTVQGEGLVLPEDEAALLPAVATQARPLTCQSLTADQRRSAIRFELYCEPVPAGETITLTLPELPRQSGGTFPALTATFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J73</accession>
    <name>Q67J73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Formate dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3296"/>
    <dbReference type="EnsemblBacteria" id="BAD42277">
        <property type="protein sequence ID" value="BAD42277"/>
        <property type="gene ID" value="STH3296"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3296"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061371_2_1_9"/>
    <dbReference type="OMA" id="DIMTICN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="134" mass="14960" checksum="F5194C94C41FD166" modified="2004-10-11" version="1">MKQVLTTCPYCGTGCTFYLNVEGDQVVGVTPNDDPRSVNQGILCLKGHFGYDFIDRQKRLTTPLIRKNGELVEASWDEAIHLVASKMKETIRDYGPDALAAFSSARCTNEENYLMQKLMRAVVGTNNVDHCARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J75</accession>
    <name>Q67J75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Electron transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hydN2</name>
        <name evidence="6" type="ordered locus">STH3294</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197406.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3294"/>
    <dbReference type="EnsemblBacteria" id="BAD42275">
        <property type="protein sequence ID" value="BAD42275"/>
        <property type="gene ID" value="STH3294"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3294"/>
    <dbReference type="eggNOG" id="COG1142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_3_0_9"/>
    <dbReference type="OMA" id="CAMDANP"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="20846" checksum="6B2698BA53488645" modified="2004-10-11" version="1">MCALNAFVVADPHKCIGCKVCEVSCFVVHSDVPVKTVGQVTTPIIPRLYLVHTAEVTMPVQCRHCEDAPCANACPVGAITQKDGIIDVDQETCIGCKTCVLACPFGAMEMVPYYKNGEQVKQLRLTEETGEGPVLQDVFVASKCDRCIGVEGGPACVANCPREALQVVRPDELRRTRNLEAALAVLESVKNVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC3</accession>
    <name>Q67JC3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197358.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3245"/>
    <dbReference type="EnsemblBacteria" id="BAD42227">
        <property type="protein sequence ID" value="BAD42227"/>
        <property type="gene ID" value="STH3245"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3245"/>
    <dbReference type="eggNOG" id="COG0746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071013_1_0_9"/>
    <dbReference type="OMA" id="ICYPIVE"/>
    <dbReference type="OrthoDB" id="1227281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="27800" checksum="64ED9D4EA8AEF86A" modified="2004-10-11" version="1">MAVHVIVLAGAANGGPLREVSSAPNEALVEINGKPMIQYVIDALQEARSIGRIVIVAPPGQLEPHVSGRNLEFVPSRDHIVDNILEALKVLQPEDRFLVISSDVPLITGQIVDDLIALCAQRPADLYYPIVERKVAEERYPGVQRTYVRLREGTFTGGNVFLVHPSIVDRVAPKVRAFLDYRKSPLKMVSLLGWGFTLRYLLLRNLSLRELEEKVSRMLDINGAVVVCPWPEVGIDVDKPSDLGLVESLLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI5</accession>
    <name>COAX1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type III pantothenate kinase 1</fullName>
            <ecNumber evidence="1">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PanK-III 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantothenic acid kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaX1</name>
        <name type="ordered locus">STH3183</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197296.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JI5"/>
    <dbReference type="SMR" id="Q67JI5"/>
    <dbReference type="STRING" id="292459.STH3183"/>
    <dbReference type="EnsemblBacteria" id="BAD42165">
        <property type="protein sequence ID" value="BAD42165"/>
        <property type="gene ID" value="STH3183"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3183"/>
    <dbReference type="eggNOG" id="COG1521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066627_1_0_9"/>
    <dbReference type="OMA" id="TCITYDF"/>
    <dbReference type="OrthoDB" id="2039419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00671">
        <property type="entry name" value="baf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000267593" description="Type III pantothenate kinase 1">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <sequence length="268" mass="28916" checksum="3311303819C0F8B9" modified="2004-10-11" version="1">MLLAVDIGNTNITAGLFLEDELICMWRMASNRAETWDEYGLQLIQLLERDGHTPADITCAAMASVVPPLNPVLSRAVQQYLKVQLKELTGDLRSLPAVRYDNPAALGTDRLVNALAGWEIYGRPQGRPVIVVDFGTATKLECVSAEGEYLGGVIAPGIRTAAEALTRRTALLQRVEIQRPKSVIGRNIAASMQSGILYGFAGLADGLVRRLTQEIAPDGPDPVVVATGGLASMVAVESRTIQMVDPHLTLQGIRLMYELNWPDAAAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK7</accession>
    <name>Q67JK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197274.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3161"/>
    <dbReference type="EnsemblBacteria" id="BAD42143">
        <property type="protein sequence ID" value="BAD42143"/>
        <property type="gene ID" value="STH3161"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3161"/>
    <dbReference type="eggNOG" id="COG1307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048251_0_1_9"/>
    <dbReference type="OMA" id="IVHHINC"/>
    <dbReference type="OrthoDB" id="1288126at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="31978" checksum="524554915DC513AC" modified="2004-10-11" version="1">MTHNRSCTLVVDGGVDLPEALIEQYGLHIVPLTVNFGDESYQSGVDMTPAAFYQRLRERGEFPTTSQPAVGDYVTAYRRAAEAGLPILSLHLSCGLSGSYNAASAARALVPELDITLVDTRTLSGEMALQVLVAAEMAEQGRSIAEIREVIEAVNASSHLYFTLDKLDYLRKGGRIGRVSAAVGGLLGIRPIVTVDKSTGTYIAAGKARSFRRAVETIAHRIIEDVGEGGQVSLVVLEGHCPDQVAHLVSLLRSRLEVVWFHHLHVNPSLGAHVGPDAIGVAYFPGELPFVGRDAAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM9</accession>
    <name>Q67JM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3139"/>
    <dbReference type="EnsemblBacteria" id="BAD42121">
        <property type="protein sequence ID" value="BAD42121"/>
        <property type="gene ID" value="STH3139"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3139"/>
    <dbReference type="HOGENOM" id="CLU_949741_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269234" evidence="1">
        <location>
            <begin position="39"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="30554" checksum="138FC636F52D286F" modified="2004-10-11" version="1">MAGNPTSRGRGPRRHWPASALAVALAAFLTVPCTAVCRASPATDAAPTARTVPAGKEAAPPEAVRTMSAAQGEGLPIPGDWIPPLPAVAGFRLVLSPPAGPGGAPQPLYATHPDHRPVIALVLDRLVGSRLTAGTAEPSAAGGVLHVALRRGGHLAVEPAGECPDAADPGERGYVCPPSPSDVVLRLSDGRAVRVRNPWMAGWLQEGWRAHLPVGAPQLLDRDAAIALAREQSGLPAWQARFVDAYPVERSGGTEVRPAWLLEAELPAGQRIRLVLDAQTGEVLRLVQLEALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN1</accession>
    <name>Q67JN1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH3137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197250.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3137"/>
    <dbReference type="EnsemblBacteria" id="BAD42119">
        <property type="protein sequence ID" value="BAD42119"/>
        <property type="gene ID" value="STH3137"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3137"/>
    <dbReference type="eggNOG" id="COG1092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_9"/>
    <dbReference type="OMA" id="DCFCHTG"/>
    <dbReference type="OrthoDB" id="468283at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI_PUA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD42119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="390" mass="43754" checksum="204E2CC66BC0FF3E" modified="2004-10-11" version="1">MAVVQLARGRHRRVQAGHPWIFQGEVEEIRGEFAPGDVVTVVDFRGRLLGKGYINPASQILVRMLTAHDEPVDREFFRRRLERAWAYRQKLLPDTGACRVVFGEADFLPGLIVDKFGDILVVQTLALGIDKWLPDIVDILVDLLRPRGIYERNDVPVRRLEGLEERKGFLRGEFDPRITIVENGLEIAVDVAEGQKTGYYLDQRENRHAVRRYAAGGRVLDCFCNVGGFALNAAAAGAREVVAVDASAEAIAAAEENAARNGLRDRIRFQVGNAFDILRQMEAQRERFDVIVLDPPAFAKNKAALEGAARGYKEINLRALRMLTEGGFLVTCSCSYHMTPDLFKAVVAEAAQDARRRLRLVEERAQAVDHPIVVGYDESHYLKCLIYEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN9</accession>
    <name>Q67JN9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197242.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3129"/>
    <dbReference type="EnsemblBacteria" id="BAD42111">
        <property type="protein sequence ID" value="BAD42111"/>
        <property type="gene ID" value="STH3129"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3129"/>
    <dbReference type="eggNOG" id="COG1605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062599_1_1_9"/>
    <dbReference type="OMA" id="PHISQGV"/>
    <dbReference type="OrthoDB" id="687380at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006268">
        <property type="entry name" value="DAHP_syn_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01361">
        <property type="entry name" value="DAHP_synth_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="1">
        <location>
            <begin position="265"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="38328" checksum="B4A692C82A541E58" modified="2004-10-11" version="1">MSVVEGLTAETKRTVAGRFAGLRLGSGRPMIIAGPCSVESEEQIVAAARAVKAAGATALRGGAFKPRTSPRSFQGLGEEGLRLLAVARAETGLPVVTEVMDTAQIDLVAEYADVLQVGSRNMQNFALLKQVGRAGKPVLLKRGLSATLEEWLAAAEYILESGNDQVILCERGIRTFETSTRNTLDLSTAILARTRSGLPVIVDPSHAAGRRDLIEPLSLAALAAGMDGLMIEVHPDPDRALSDAAQQLNFEQFSRLMARLELLPASALESIDACREEIDRLDERILDLLLRRMAAARRVGQLKRGAGLPVLQEDREHALINRLAGLAGDELAGEEVREIWQAILAVSRRAQQALHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT1</accession>
    <name>RL10_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplJ</name>
        <name type="ordered locus">STH3087</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JT1"/>
    <dbReference type="SMR" id="Q67JT1"/>
    <dbReference type="STRING" id="292459.STH3087"/>
    <dbReference type="EnsemblBacteria" id="BAD42069">
        <property type="protein sequence ID" value="BAD42069"/>
        <property type="gene ID" value="STH3087"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3087"/>
    <dbReference type="eggNOG" id="COG0244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092227_2_0_9"/>
    <dbReference type="OMA" id="VRDQKQA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002363">
        <property type="entry name" value="Ribosomal_L10_eubac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01109">
        <property type="entry name" value="RIBOSOMAL_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154729" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <sequence length="179" mass="19217" checksum="4FC05E321AF4C8A3" modified="2004-10-11" version="1">MRTIRPEKQQAVAELKEALQNAKSVVLADNLGLTVAQVTQLRRELRQAGVELKVAKNTLIGIAARELGIEGLEPYLHGTTTLAFSYEDEAAGAKKIREFFAKEREPKFVMKAGILEGKVIDADGVKALADLPNRETLLAQVLAGIQAPLQGVAGAINGLLASFAYALDARIRQLEGAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K63</accession>
    <name>Q67K63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2952"/>
    <dbReference type="EnsemblBacteria" id="BAD41935">
        <property type="protein sequence ID" value="BAD41935"/>
        <property type="gene ID" value="STH2952"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2952"/>
    <dbReference type="HOGENOM" id="CLU_1577702_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="17648" checksum="A33291A82771E49B" modified="2004-10-11" version="1">MTPMSHFYPPPMRGPAWYPPAGFGAPAWYPYTAAYALYPPAAMAPAGWYAVADRPPAPDPAPAEWMALVEPALNSVIPAIHGLLEISGLLLRHLPAAFDMTGSGPEEPARDDPLTMRTGLGHIGLFGPAEPDVIDIEAARSDAALPPPEGNGRDGAGAADLSGDQTEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA7</accession>
    <name>Q67KA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2908"/>
    <dbReference type="EnsemblBacteria" id="BAD41891">
        <property type="protein sequence ID" value="BAD41891"/>
        <property type="gene ID" value="STH2908"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2908"/>
    <dbReference type="eggNOG" id="COG3039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070817_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="123"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="338" mass="38833" checksum="206DA1BA286D55FA" modified="2004-10-11" version="1">MVARPVYHAVALILQHLHRLLDEVAAERRGRKPRNQPKTGRPRLYSTPSLLLFRIAADLLGWSLRKTLNYAEQEAGAWLRKALGWEQVPHLSVVCRRLHGDDVRRLRTRLWRKLRRRSLRQGDLRVLTIDMTDLPVPLRRKDPDATWGATSKGKFFGYKLHLVISRTGFPLAFRVTPAAIHEMRPAVSLFAEVVKLADQKQKAELTYGVGDSAYDSENLYKFWADVCCGHFLCAPNRRGTPEGEEVRAQGPYRKAALEFWKTDLARRIYGKHRSLIESVNAQLKDLSNPFQVGQIPGWIRGLRGVSDWVFWRILAFSSILYANRLRGSCSRKLSRFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM4</accession>
    <name>Q67KM4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196908.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41774">
        <property type="protein sequence ID" value="BAD41774"/>
        <property type="gene ID" value="STH2789"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2789"/>
    <dbReference type="HOGENOM" id="CLU_2023530_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="127" mass="13103" checksum="74C8E30CFC569417" modified="2004-10-11" version="1">MASVYALAADLFFAERLERALRDLGHKPEVRDLSAGGSGQAPGNAAITQVSLPGGVDLAIVDLEAGEAALALVRAARERGIPVLAFGAHVDVEARRAAEGLGARVVTKSRLTRSFTDLVAAMLGPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM9</accession>
    <name>Q67KM9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AhpC-TSA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2784"/>
    <dbReference type="EnsemblBacteria" id="BAD41769">
        <property type="protein sequence ID" value="BAD41769"/>
        <property type="gene ID" value="STH2784"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2784"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2557043_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AhpC-TSA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="8801" checksum="F575BF1A7D3DBABA" modified="2004-10-11" version="1">MAVNPGSLASHQRWAEKNRFGFPICADEGKHVARAYGVLNALGFIARTVVLVDRNGIVRWVQPGMPATETILAAVDALGEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN4</accession>
    <name>Q67KN4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2779"/>
    <dbReference type="EnsemblBacteria" id="BAD41764">
        <property type="protein sequence ID" value="BAD41764"/>
        <property type="gene ID" value="STH2779"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2779"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_943110_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="33831" checksum="D7D9E6DC7DD72CC5" modified="2004-10-11" version="1">MPLSRLRRLLEEERYREAQLEGERLIHAGTLTAEELAHAYKGTAMASYYRREVYAAIKLGEYALAVAERTTNWELINKCRYDLGEYYLTLGDYPQAFDHLFQCLDDLHRTPHLMEVEAGTHHKLALLFRYRRQYADAIGSHHLSLDLLRRCGHWPLLMEALRGLVYCYLALDQPDEALPYLEQLEHLLGEHPNDQVAASLLTDWAYYHQRTGDLRQSMAFCTRAMAPGLPGQDENVLSTASVIAGENALSLGRYDEAQLFANLAMQYALESRQSALMNRAAALRRKLYERGQARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR1</accession>
    <name>Q67KR1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2751"/>
    <dbReference type="EnsemblBacteria" id="BAD41736">
        <property type="protein sequence ID" value="BAD41736"/>
        <property type="gene ID" value="STH2751"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2751"/>
    <dbReference type="eggNOG" id="COG1178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_1_0_9"/>
    <dbReference type="OMA" id="AWRWVAL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="71"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="362"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="567" mass="62419" checksum="2CEF4492CFE3E3D9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR8</accession>
    <name>Q67KR8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2744</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2744"/>
    <dbReference type="EnsemblBacteria" id="BAD41729">
        <property type="protein sequence ID" value="BAD41729"/>
        <property type="gene ID" value="STH2744"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2744"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_9"/>
    <dbReference type="OMA" id="WRHAFPT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="34699" checksum="B866D0D62E3EDAD0" modified="2004-10-11" version="1">MAGFILRRLLQLVPMLLGASLLIFAVIRLAPGDPYSGLVDPRADPAKIEELRRRHCLDCPLPQQYWRWLSHAARGDLGTSIRFRIPVAQLIGDRLGPSIALGVTAQSIVLLAGIPFGILAAVRRGTWVDAAVTTCSLAFLAIPAFFLGLLLLRFFSLGGLQVLPSAGFTTPGSLLTGPARFLDAARHLILPATALGVTGIAGLMSHVRSAMLEVLRQDYLRTARAKGLPERMVIYRHALRNALMPVITLLGLNLPALLGGAIITESLFQWPGVGRLTYTALLERDYPVLMALNLLFAALTLLGSLLADVGYAAVDPRVRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT9</accession>
    <name>Q67KT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2722"/>
    <dbReference type="EnsemblBacteria" id="BAD41707">
        <property type="protein sequence ID" value="BAD41707"/>
        <property type="gene ID" value="STH2722"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2722"/>
    <dbReference type="HOGENOM" id="CLU_1947742_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="14190" checksum="1A41EB24696DCA1F" modified="2004-10-11" version="1">MDALEADDKVYDLPLINYAVNIHDQTTGFHIVLPENWPALVHRAVAPARYIGPVTVISLRGGNKNLREAVHIDESGKAVPAGPVIVSGRNVSRELPSAWATGESRPWGWSRDARRPPTPRWTTPHACGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L86</accession>
    <name>Q67L86_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ordered locus">STH2575</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196697.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2575"/>
    <dbReference type="EnsemblBacteria" id="BAD41560">
        <property type="protein sequence ID" value="BAD41560"/>
        <property type="gene ID" value="STH2575"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2575"/>
    <dbReference type="eggNOG" id="COG0024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_1_9"/>
    <dbReference type="OMA" id="LVVIKTW"/>
    <dbReference type="OrthoDB" id="1285136at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="12"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="26907" checksum="FF12D38D1FAF30C1" modified="2004-10-11" version="1">MIRLKSQREIAIMRDAGRIVAECHAALAEKIEPGITTWELDQFVEQFLARHGATPSFKGYNGFPASICASVDDVICHGFPSRKRLKAGQVVTIDIGAYYKGYHGDSAWTYAVGEVSPSVQELMKVTEEALYAAIAVALPGNRMGDIGHAIQSLAESHGFGVVREYIGHGVGQKLHEPPEVPHYGQPGTGIALRPGMTIAIEPMITLGDWRSKLDPDGWTARTVDGSICVQFEHTIAITDNGPEILTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD8</accession>
    <name>Q67LD8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein, histidine-rich</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196646.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2523"/>
    <dbReference type="EnsemblBacteria" id="BAD41508">
        <property type="protein sequence ID" value="BAD41508"/>
        <property type="gene ID" value="STH2523"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2523"/>
    <dbReference type="eggNOG" id="ENOG5032T7R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138370_0_0_9"/>
    <dbReference type="OMA" id="DWIVAFE"/>
    <dbReference type="OrthoDB" id="1917919at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019635">
        <property type="entry name" value="DUF2500"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10694">
        <property type="entry name" value="DUF2500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="126" mass="14549" checksum="C3316032FC5F46B8" modified="2004-10-11" version="1">MSDPFFGGGAFRLFDIMFVLVPILVIGGFVFVFGSMIYTAVSNIQKPLLTRRARVVAKRVRHSTHHHHHGDHHHHSHHTAYYVTFEFEDGRREEFSVRGDQYGLLVEGDEGTLQSQGTWFKGFYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF0</accession>
    <name>Q67LF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196634.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2511"/>
    <dbReference type="EnsemblBacteria" id="BAD41496">
        <property type="protein sequence ID" value="BAD41496"/>
        <property type="gene ID" value="STH2511"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2511"/>
    <dbReference type="HOGENOM" id="CLU_946374_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="31179" checksum="914F61C36408C5C4" modified="2004-10-11" version="1">MYQHIHYPDAPAPRSLPRWTVWAFAPVALALGLIVGVAASAYGPWSAPAGEPAVQAPPEQETPAPADSTALPLPATGNVPTQTPGPEAPSAVGTAPLLPPAVDDSTYVTPVPVTAGPSLAEVRTLVDARTVLASEFGTFTAGGRQWFQVDYQMARDINYDTMLVGIVKIADYNNWLTAVRDYRDELTRWLTAAARRVRDAAQNEGFKLSWALFEVVPEPPYGFLASEVTQMPRGQGYLVTRPLAAVSDFSGPTVTLAAVAGTGGGPVTDPSPASVYAPVLRFDPTDLYRPPTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL2</accession>
    <name>Q67LL2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196572.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2449"/>
    <dbReference type="EnsemblBacteria" id="BAD41434">
        <property type="protein sequence ID" value="BAD41434"/>
        <property type="gene ID" value="STH2449"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2449"/>
    <dbReference type="eggNOG" id="COG3394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064244_4_0_9"/>
    <dbReference type="OMA" id="IGRDYGM"/>
    <dbReference type="OrthoDB" id="1204930at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006879">
        <property type="entry name" value="YdjC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31609">
        <property type="entry name" value="PTHR31609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04794">
        <property type="entry name" value="YdjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="276" mass="29926" checksum="096ED90130B0AC4A" modified="2004-10-11" version="1">MRGLIVNADDFGLSPGVSRGILRAHWEGIVTSTTFMVNFPWAAEMAGLLRRAPRLGVGLHLNLTTGAPVLPPAEVPSLVGPDGRFGRSLVRLLARMRPAEAEREWSAQVERFVALMGRPPTHLDTHRYLQAYPPLCRAMIRVARRYGVPAVRVLPPGVFPEGTFPPWSPAGPLLGVALRRTTALIRASGLAAPDRALAGDFDLPGLLTRLDRVGDGVTELVTHPGEVDDLLRSLSSLQEQREVELAALTSPAARERVQARGIRLMHFGDLTDAARG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN9</accession>
    <name>SYH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="1">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisS</name>
        <name type="ordered locus">STH2422</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196545.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LN9"/>
    <dbReference type="SMR" id="Q67LN9"/>
    <dbReference type="STRING" id="292459.STH2422"/>
    <dbReference type="EnsemblBacteria" id="BAD41407">
        <property type="protein sequence ID" value="BAD41407"/>
        <property type="gene ID" value="STH2422"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2422"/>
    <dbReference type="eggNOG" id="COG0124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_1_1_9"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="OrthoDB" id="277998at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136275" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <sequence length="432" mass="48655" checksum="45446B35F1A0A967" modified="2004-10-11" version="1">MSIQAPRGFNDILPGEQYGWRDSYRWQRLEEIFREVARLYGYQELRPPMVEYVDLFIHGVGATTDIVTKEMFNITPRGDDPDARRMAMRPEFTAGLVRAWLENGLYNNPQPTKIFAYGPAFRYENVQKGRFRGFHQLDVEVFGAQDPAVDAEVIKLGLDVVARLGLTGLVVSVNSIGCPQCRPRYRQALQDHFRPHLGELCEDCNTRFEKNPLRLLDCKRDADHPAQRTAPVTLDYLCDDCRRHWEGLLSHLAAMGIPYQIDTRIVRGLDYYTKTVFEVLHPKLGAQSALWGGGRYDGLIEIVGGKPTPGVGFGMGMERVLMVLEEEGLTAPFADRPRLDVFVATLGEAARPVGLKLLYALRDAGLSADIDYLGRSLKAQMKYAGKQNSRYVVILGEDEVRQGVASVKHMDEGTQESVPLDQIISHLRRAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR2</accession>
    <name>Q67LR2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2399</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196522.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2399"/>
    <dbReference type="EnsemblBacteria" id="BAD41384">
        <property type="protein sequence ID" value="BAD41384"/>
        <property type="gene ID" value="STH2399"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2399"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_9"/>
    <dbReference type="OMA" id="IIFIDIM"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13543" checksum="66B22B2533F3DD41" modified="2004-10-11" version="1">MALRILLIEDDPDIQRMVQLSLKFQGGHEVSVASGGQEGIAKAEQERPDLILLDVMMPEMDGYETCRRLKANPATASIPIVFLSARAQQSEIEKGRSLGAIGYLVKPFDPMTLSDQLEAMLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU2</accession>
    <name>Q67LU2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2369"/>
    <dbReference type="EnsemblBacteria" id="BAD41354">
        <property type="protein sequence ID" value="BAD41354"/>
        <property type="gene ID" value="STH2369"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2369"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052868_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="PTHR43675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="93"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="278" mass="28989" checksum="981E955AA380E003" modified="2004-10-11" version="1">MWMDAHALREMVRKRYAEAARGSAAGSAGGCCAPEPKGDDLLVVSGCCCGSGAGGEELGRVLGYSDDELAAAPAGADLGLGCGNPQAIASLKPGETVLDLGSGAGFDCFLAARQVGESGRVIGVDMTPEMLARARENARKGGFANVEFRLGEIEHLPVADESVDVIISNCVINLSPEKEQVFREAFRVLRPGGRIAVADMVSLAPLPPEVREDLALYAGCVAGVATVGELRTMLTEAGFVDIVIRPKDSEQLLEAWGAGESLKNQVFSATIEARKPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY1</accession>
    <name>Q67LY1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anaerobic dimethyl sulfoxide reductase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dmsC1</name>
        <name evidence="2" type="ordered locus">STH2330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2330"/>
    <dbReference type="EnsemblBacteria" id="BAD41315">
        <property type="protein sequence ID" value="BAD41315"/>
        <property type="gene ID" value="STH2330"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2330"/>
    <dbReference type="eggNOG" id="COG3302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064909_1_1_9"/>
    <dbReference type="OMA" id="YASVPAW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019645">
        <property type="term" value="P:anaerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007059">
        <property type="entry name" value="DmsC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38095">
        <property type="entry name" value="PTHR38095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04976">
        <property type="entry name" value="DmsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="282" mass="30059" checksum="ADBC503F3271D50A" modified="2004-10-11" version="1">MHDWSLILYTLCLQSSVGIYVASRLVLWNAESKEARMRFLYLALGLGIAGVLASLTHLGYPLNALKTMGNLGTSWLSREILLTLLFGVTGIASVVLERQEVGSRETRNGWAVLTSLAGLALIYVMSMIYRTTAFPAWTHVSTTLVFYATAGLIGTSAVFAGLCCRTGGEEPRGLMGLVVGAMAMLALQVMALALHGVYLGTAGPEAQATAALIAGEWSALYWSQIVCIAAGTGIMMPLVWRRVAQKKLANMPQFAGALVALVALGELAGRVVFYATRVKIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY5</accession>
    <name>Q67LY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2326"/>
    <dbReference type="EnsemblBacteria" id="BAD41311">
        <property type="protein sequence ID" value="BAD41311"/>
        <property type="gene ID" value="STH2326"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2326"/>
    <dbReference type="eggNOG" id="COG2071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030756_2_0_9"/>
    <dbReference type="OMA" id="ECVILPT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="PTHR43235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="29200" checksum="A7DA945222C6F107" modified="2004-10-11" version="1">MTASLRAAGPADRRPARKPIIGITPSFDRECDRPLISGRPLLYVTQENVRAIRAAGGHAVILPTCLDDEDDLLDQLDGLLVTGTETPLPRRLRQREQLPGLREQNPERYDSDSRWLRGALARRMPVLAICHGMQMLNDILGGSLWPRIFPKNESERHAQTAPPDQPWHPLHVLPGSLLAASLGVTETMVNSFHVQAVRRPGDGVQVTGFSADGVVEAIECPGYPFVIGVQFHPERLVRSNPHMLNLFRAFVSAAAAYAGERCAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M01</accession>
    <name>Q67M01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase C-terminal subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2310"/>
    <dbReference type="EnsemblBacteria" id="BAD41295">
        <property type="protein sequence ID" value="BAD41295"/>
        <property type="gene ID" value="STH2310"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2310"/>
    <dbReference type="eggNOG" id="COG3958">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_9"/>
    <dbReference type="OMA" id="VINTCDY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="3"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="32141" checksum="CCAE845B1A686457" modified="2004-10-11" version="1">MTVAMRDAYGEALAQLGGLRPDVVVLDADLGNSVRCDGFGRLYSDRYIQVGIAEQNMVGVAAGLAACGLVPVVNSFAAFAVCRALDQIRVSVCQTGLPVKVVGSYSGLAVSKGGSTHASVEDIAVMRALPGMTVIVPGDAEEAAQVTRMLPDIPGPVYLRLYRNAVPPVVPAGYRFRPGKAVLLRPGTDVAIVSTGTMTARALEAAGRLAGRGVGAAVLHVPTVKPLDEEAVVDVAARCRAVVTAEEHSVIGGLGAAVAECLGERHPMPVHRVGVPDRFGESGPDDELLAHLGVDEAAIERRVLAALGEGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M34</accession>
    <name>Q67M34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41261">
        <property type="protein sequence ID" value="BAD41261"/>
        <property type="gene ID" value="STH2276"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2276"/>
    <dbReference type="HOGENOM" id="CLU_1282671_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="23767" checksum="F43ED313A173B5F8" modified="2004-10-11" version="1">MPLLLRRNVGIDSPLQEPLNVLLTEVPCIGRNLLRHLIDIGFDLLHHGDQVRSICRLVCYGSSDDNLRIGIDDGLGVVPLDELLIRAFHDPGIGIRKVALRCWCGFCLRPLPPTTALGPLGSRLLGRLMFVSAGPQAHFGFQSSLGFLNLGQPPLTEAEFFGQFIPTTMWAEAVILGLVSLLGFLEQLCDLRLQALFFLHHPAVSSSPCAWRRWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M93</accession>
    <name>Q67M93_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2215"/>
    <dbReference type="EnsemblBacteria" id="BAD41200">
        <property type="protein sequence ID" value="BAD41200"/>
        <property type="gene ID" value="STH2215"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2215"/>
    <dbReference type="eggNOG" id="COG1479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1874386_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004919">
        <property type="entry name" value="DUF262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="DUF262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF262" evidence="2">
        <location>
            <begin position="51"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="15409" checksum="349F125020738C51" modified="2004-10-11" version="1">MHEVRVIHAKLELAGERNHRPRCLTNTHANVRSGWPRRSREVEHGVKISIILSHIDTGHIALPEFQRGYVWNRDQVHGLFDSLCRRHPIGGLLVWVTEAKSAVHRGDGPPGLRRPQAPTGRPSARHIWSVGRRVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB3</accession>
    <name>Q67MB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196320.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2195"/>
    <dbReference type="EnsemblBacteria" id="BAD41180">
        <property type="protein sequence ID" value="BAD41180"/>
        <property type="gene ID" value="STH2195"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2195"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_1_9"/>
    <dbReference type="OMA" id="SIFVMQG"/>
    <dbReference type="OrthoDB" id="1841075at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="286" mass="30107" checksum="B8A4518A424222D7" modified="2004-10-11" version="1">MGLIAWLTLQGLTVGMLWFLIASGLSLIFGLMNVLNFAHGGLYMLGTYGALTLYRATGSFPLALVGGALLGALLGAAIELLAIRPLYGRQLFQVLMTLGVMLIFDEAVEAIWGPHPLPFQTPALLSGTLQVFDRPFPVYRLFIIAVGLLVYLAVHLFLTRTRLGVIVRAGVENREMVQTLGVDIRKVFTGVFALGGTLAALGGAVAGPFEGAHPTLAAENLLPAFIVVVVGGLGSFTGSLLAAILVGLLQAFVGFYAPQLALAVNIALMAVVLVLRPQGLLGEQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD3</accession>
    <name>Q67MD3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA repair exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sbcC</name>
        <name evidence="3" type="ordered locus">STH2175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196300.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2175"/>
    <dbReference type="EnsemblBacteria" id="BAD41160">
        <property type="protein sequence ID" value="BAD41160"/>
        <property type="gene ID" value="STH2175"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2175"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_1_2_9"/>
    <dbReference type="OMA" id="HACPVCE"/>
    <dbReference type="OrthoDB" id="1143316at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_23" evidence="2">
        <location>
            <begin position="6"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="523"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="603"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="824"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1200" mass="133921" checksum="5E91C379CF607FF5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF5</accession>
    <name>LIPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl synthase</fullName>
            <ecNumber evidence="1">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lip-syn</fullName>
            <shortName evidence="1">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipA</name>
        <name type="ordered locus">STH2153</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MF5"/>
    <dbReference type="SMR" id="Q67MF5"/>
    <dbReference type="STRING" id="292459.STH2153"/>
    <dbReference type="EnsemblBacteria" id="BAD41138">
        <property type="protein sequence ID" value="BAD41138"/>
        <property type="gene ID" value="STH2153"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2153"/>
    <dbReference type="eggNOG" id="COG0320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_1_9"/>
    <dbReference type="OMA" id="PYCDIDF"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102552">
        <property type="term" value="F:lipoyl synthase activity (acting on glycine-cleavage complex H protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102553">
        <property type="term" value="F:lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="PTHR10949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000325315" description="Lipoyl synthase">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="68"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="320" mass="35798" checksum="ACA819E1EA2B3D56" modified="2004-10-11" version="1">MAEFRIDPEMELPVLPSRARADVSRKPEWLKINLRTDAEFVELKRLMRGQGLHTVCEEARCPNIFECWNRRTATFMILGDICTRNCGFCAVRSGVPTGLDLAEPERVADACVQLGLRHVVVTSVARDDLSDGGASIFAETIRAIRRKNPFTGVEVLIPDFGGNWDALAVVMDAEPDVLNHNIETVRRLSDRVRSRAKYDRSLELLRRAKEMKPHVSTKSSIMVGLGETMQELYEAMDDLRAAGVDIVTFGQYLRPTARHLAVEKFYTPAEFEHLREEALKRGFAHCESGPLVRSSYHADEQSAQAVARRTGAGRAAQTGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ9</accession>
    <name>Q67MJ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2109"/>
    <dbReference type="EnsemblBacteria" id="BAD41094">
        <property type="protein sequence ID" value="BAD41094"/>
        <property type="gene ID" value="STH2109"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2109"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018243_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="746" mass="78506" checksum="3B04C1510150DE34" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK5</accession>
    <name>Q67MK5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2103</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2103"/>
    <dbReference type="EnsemblBacteria" id="BAD41088">
        <property type="protein sequence ID" value="BAD41088"/>
        <property type="gene ID" value="STH2103"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2103"/>
    <dbReference type="eggNOG" id="COG3714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079086_6_0_9"/>
    <dbReference type="OMA" id="ACFKCLP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="YhhN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="PTHR31885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="212" mass="22476" checksum="050602D0A51A645D" modified="2004-10-11" version="1">MLEPLSVAIGLAGMGDLFAIWRGIRWLRYVFKPGTMLLIIALAATGLPEAGAFGYLVLAGLLFSVAGDVFLVLPQNRFLAGLVSFFCAHVLYIAAFAVRRAGWGLLDGALLAGLAAAGVLFFRRMRPGVRREGGNRMLLATGVYLAAITLMVAAAVGTRLPATAAGALLFYLSDAILAWNRFVRPLPRANLGVMSAYFLGQYLIAVSVLIGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML5</accession>
    <name>COXX_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB</name>
        <name type="ordered locus">STH2093</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with CtaA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196218.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67ML5"/>
    <dbReference type="SMR" id="Q67ML5"/>
    <dbReference type="STRING" id="292459.STH2093"/>
    <dbReference type="EnsemblBacteria" id="BAD41078">
        <property type="protein sequence ID" value="BAD41078"/>
        <property type="gene ID" value="STH2093"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2093"/>
    <dbReference type="eggNOG" id="COG0109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_2_9"/>
    <dbReference type="OrthoDB" id="1875497at2"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327170" description="Protoheme IX farnesyltransferase">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <sequence length="298" mass="32820" checksum="485C58768AC57C6F" modified="2004-10-11" version="1">MFAQARDRLQSGQVVRDYVALTKPRIVILLLITGFAAMWVAAGGPPPLGLTVVTMIGLALSCGAANAINMWYDRDIDAVMARTRRRPLPAGRLTPEQALRFGVITGALSFLVLLTVNLLTALLATAGLLFYVLVYTMWLKRSTVHNIVIGGAAGAAPPLVGWAAVTGRLDWAAVIMFLVVFLWTPPHFWALALFRSEDYERAGVPMLPVVRGERATKWQILLYSLLLIPSAALLYWTGTVGRLYLWTSVVLGCAMVSASVGLLRERAPQMDWAHRTYGWSLLYLFVIFLAMMLDVTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN0</accession>
    <name>Q67MN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196203.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41063">
        <property type="protein sequence ID" value="BAD41063"/>
        <property type="gene ID" value="STH2078"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2078"/>
    <dbReference type="eggNOG" id="ENOG502Z7KY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124273_0_0_9"/>
    <dbReference type="OMA" id="FCDRRYD"/>
    <dbReference type="OrthoDB" id="1197155at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025352">
        <property type="entry name" value="DUF4256"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14066">
        <property type="entry name" value="DUF4256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="188" mass="21692" checksum="A67F93B5380253DC" modified="2004-10-11" version="1">MISPERTLTPEQRDELIRTLKTRFERNMHRHPGLDWTDVQARLEARPEKLWSLHEMERTGGEPDVVGFDSQTGEYIVYDCAPESPKGRRSLCYDHAALESRKQHKPEGSAVGMAEAMGIELLTEQEYRELQKLGAFDTKTSSWVRTPPEIRRLGGAVFCDRRYDTVWVYHNGAESYYASRGFRGSLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR6</accession>
    <name>Q67MR6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196168.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2042"/>
    <dbReference type="EnsemblBacteria" id="BAD41027">
        <property type="protein sequence ID" value="BAD41027"/>
        <property type="gene ID" value="STH2042"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2042"/>
    <dbReference type="HOGENOM" id="CLU_1414531_0_0_9"/>
    <dbReference type="OrthoDB" id="2001051at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="20873" checksum="C6DDEEBEA7644D35" modified="2004-10-11" version="1">MHPTSGIQQVPGGWTGHGKVYPYEFRSQKIPGGAGTCRSPLEERLFRALARNPAVLSFAVRPMRIAFHVGPKAHHFTPDVLVQYADGRKVLVVVKPGQTVPDPAEQAMFKAAADYAAAHGMAFEVWTGPRWVSATDPRGGNGDFDIATAARLQTEAAAADQPHRREPAKGTLTFWTVIVVVVVLVMLMRLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU9</accession>
    <name>Q67MU9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GSDH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2009"/>
    <dbReference type="EnsemblBacteria" id="BAD40994">
        <property type="protein sequence ID" value="BAD40994"/>
        <property type="gene ID" value="STH2009"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2009"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012344_3_0_9"/>
    <dbReference type="OMA" id="ANLGWSC"/>
    <dbReference type="OrthoDB" id="567019at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GSDH" evidence="2">
        <location>
            <begin position="92"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="431" mass="46656" checksum="3C241B95C80F2EB7" modified="2004-10-11" version="1">MEQWQTGGFDLSAYLRGAVTVLLLVLLGCSPAPPPGQGTAPDQVGGQAQEHVQNPAQEPAQEPAQEPAQEQGQPAPRKPVRIGLQKVVDGLERPLYVTHAGDSSGRLYILEQPGRVRVLEGGALLETPFLDLTDRVLSRGNEQGLLGLAFAPDFRESGVFYVHYTGRPDGRTVLSRFRLLDGDPRRGDPSSEEVLLTVDQPYANHNGGALLFGPDGYLYLALGDGGSAGDPGNRAQSLDSLLGKILRLDVSQPGPYRIPPDNPFVGRQGRDEIWAYGLRNPWRISFDRATGDLWIADVGQDAVEEVNLQPAGSPGGENYGWRVWEGSRRYGQGEAPGAVFPVAEYTHAEGGCSITGGYVYRGQAVTGLVGTYLYGDYCTGYIWGLRRQDGEWRAERLLDSGLNITSFGEDEAGEVYVVDRNGAVYRIVAAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV8</accession>
    <name>SYA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">STH2000</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196127.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MV8"/>
    <dbReference type="SMR" id="Q67MV8"/>
    <dbReference type="STRING" id="292459.STH2000"/>
    <dbReference type="EnsemblBacteria" id="BAD40985">
        <property type="protein sequence ID" value="BAD40985"/>
        <property type="gene ID" value="STH2000"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2000"/>
    <dbReference type="eggNOG" id="COG0013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_1_1_9"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="OrthoDB" id="91428at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075225" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="571"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <sequence length="872" mass="96315" checksum="993BDEAB6D9E3B49" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX9</accession>
    <name>Q67MX9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Type-5 uracil-DNA glycosylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1979</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 5 (UDGb) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1979"/>
    <dbReference type="EnsemblBacteria" id="BAD40964">
        <property type="protein sequence ID" value="BAD40964"/>
        <property type="gene ID" value="STH1979"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1979"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083279_0_0_9"/>
    <dbReference type="OMA" id="CVPPQNK"/>
    <dbReference type="OrthoDB" id="1529383at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033958">
        <property type="term" value="F:DNA-deoxyinosine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10031">
        <property type="entry name" value="UDG-F5_TTUDGB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044147">
        <property type="entry name" value="UdgB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="10">
        <location>
            <begin position="72"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="28179" checksum="A86AAD5D17B7878C" modified="2004-10-11" version="1">MNLSAPHPGPAAPGCADHPPHLVPERTRCLEGLHRDIIECDRCPRLRTYCAEVGQTRVRRYRDQEYWARPVPGFGDPEARLFILGLAPGAHGANRTGRMFTGDDSGRWLYGALYEFGFADRPESVSRDDGLTLTDAYISNVVRCAPPGNKPSPGEIAACRPFLEAELAMLTRVRVVLALGRIAFDTYVRLRRAQGFDPGRIDFRHGAEYRIPGLPVLLSSYHPSRQNTNTGVLTAPMWREIFARARALLDEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N05</accession>
    <name>Q67N05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196080.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1953"/>
    <dbReference type="EnsemblBacteria" id="BAD40938">
        <property type="protein sequence ID" value="BAD40938"/>
        <property type="gene ID" value="STH1953"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1953"/>
    <dbReference type="eggNOG" id="COG2179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056221_4_0_9"/>
    <dbReference type="OMA" id="VLNPYHI"/>
    <dbReference type="OrthoDB" id="1472357at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PTHR19288:SF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="18990" checksum="5E753DB8A6F5371C" modified="2004-10-11" version="1">MGGLRPQEYHNSIFEIDLDRLRRMGKRAIILDLDNTLVRWNDPTPTPKLLEWLAAVRAHGLMPCIVSNNSGPRVGEFAARAGVPFVPSAAKPRVKGFRQAMRQLGVAPHETVVVGDQLFTDVLGGNRAGAYTILVVPIDRREFIGTRLVRLIERRVLRYLFRQGLLTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC3</accession>
    <name>Q67NC3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">recN</name>
        <name evidence="8" type="ordered locus">STH1835</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">May be involved in recombinational repair of damaged DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RecN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195963.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1835"/>
    <dbReference type="EnsemblBacteria" id="BAD40820">
        <property type="protein sequence ID" value="BAD40820"/>
        <property type="gene ID" value="STH1835"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1835"/>
    <dbReference type="eggNOG" id="COG0497">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018297_3_1_9"/>
    <dbReference type="OMA" id="QVICVTH"/>
    <dbReference type="OrthoDB" id="1074831at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003128">
        <property type="entry name" value="RecN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00634">
        <property type="entry name" value="recN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="7">
        <location>
            <begin position="2"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="343"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR003128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="596" mass="65589" checksum="462DA02418E03907" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH8</accession>
    <name>Q67NH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195908.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1780"/>
    <dbReference type="EnsemblBacteria" id="BAD40765">
        <property type="protein sequence ID" value="BAD40765"/>
        <property type="gene ID" value="STH1780"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1780"/>
    <dbReference type="HOGENOM" id="CLU_1119704_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="27774" checksum="4497F0DD4C21DC2B" modified="2004-10-11" version="1">MHWWEETEARIHRTTSAATLGQGLWQRLVQAGLEPDAAYLAAYDLAALQLLCADLVKLVDSLLEVADGDRAGWRRHALALLRWANSAESWARETAAATNRLLDSLDLEPSTLAAREEVEAEPNGALSPEEEAKLDGRYRHWHLLYERLDLKLSTMGLEEAVQRAVARTLARLYEEAVVTFRLLSGLSRESRPRYGQVARLLLQINTTWHFDLGPYLLGLGQARPDGRGTVGLATWLLLASREPDMHMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK0</accession>
    <name>Q67NK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1758"/>
    <dbReference type="EnsemblBacteria" id="BAD40743">
        <property type="protein sequence ID" value="BAD40743"/>
        <property type="gene ID" value="STH1758"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1758"/>
    <dbReference type="eggNOG" id="COG3679">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1854220_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010368">
        <property type="entry name" value="Com_YlbF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023378">
        <property type="entry name" value="YheA/YmcA-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06133">
        <property type="entry name" value="Com_YlbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158622">
        <property type="entry name" value="SSF158622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="138" mass="15043" checksum="4B14AD226A491B55" modified="2004-10-11" version="1">MGGDDVAVATRRDVWLLARELAEAIAETPEVQEYRRTEDAVLADPDAVALIREYEAAKRAVKLSRGRPPEEQKALVERFLAIEERFNAHPVIQAYWNARVALDAFMERINAVVTFPITGETAPRAKGGCGSGSCGCGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM3</accession>
    <name>Q67NM3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1735</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1735"/>
    <dbReference type="EnsemblBacteria" id="BAD40720">
        <property type="protein sequence ID" value="BAD40720"/>
        <property type="gene ID" value="STH1735"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1735"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136034_3_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="57"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="111" mass="12260" checksum="1C3302808DE506D7" modified="2004-10-11" version="1">MAAHSAQIGVRRVRRRTPKAVRRARTLTVLFLLTVAAAVLLCPRRVQTQAHEPPAGVHHTVAPGDTLWDIAVTYGGNRDAREVVYEIQRLNGLPSAEIYPGQVLFVPTESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR0</accession>
    <name>Q67NR0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AP_endonuc_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195826.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1698"/>
    <dbReference type="EnsemblBacteria" id="BAD40683">
        <property type="protein sequence ID" value="BAD40683"/>
        <property type="gene ID" value="STH1698"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1698"/>
    <dbReference type="eggNOG" id="COG1082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081219_0_0_9"/>
    <dbReference type="OMA" id="IMFEHRS"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="63"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="35247" checksum="30DC854840D8F998" modified="2004-10-11" version="1">MAQLFVPLHGAFGRVAGSGVNTYGESLERPHWEGAEIGLCDTFPDVLGAVAEAEYYEWVIGAHHPTHREGGPAWCRFLDPDPAVRTEALEVACSAAQAAHRLGARYILYHFPWPALQMPGADYAALGWRFRDADPIPMEAWPREADLYDWSRRCFDRLAELQERERIRVVLEIDGPNPHFFDGELYGRLFEEFPDLSLCLDTGRLGLLARTHGQDPLALAARWLPWTRYLHLHTSFWDEQGRFHNHVPTRGSHTRDLWPRVTPAADIARMVVEAQPRAVIVLEHNPQTVTPQELEACHEWAATLAGRSPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR6</accession>
    <name>Q67NR6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome D ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH1692</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome ubiquinol oxidase subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1692"/>
    <dbReference type="EnsemblBacteria" id="BAD40677">
        <property type="protein sequence ID" value="BAD40677"/>
        <property type="gene ID" value="STH1692"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1692"/>
    <dbReference type="eggNOG" id="COG1271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_2_9"/>
    <dbReference type="OMA" id="WAIIFNP"/>
    <dbReference type="OrthoDB" id="271551at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="PTHR30365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0349">Heme</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="420" mass="45639" checksum="801DEA9239E32554" modified="2004-10-11" version="1">MAYSLFFHMIFAALGVGMPALLATADYLHLRTRDRDYERLAKTWARATAVLFAIGAVSGTGIAFELGLLWPEFMHFAGSTVGPAFTLEGYAFFTEAIFIGLYLYGRERMRPVAHWLTAVAVAVSGAASSFLVTAANAWQQNPVGVDLLQSNPEAMNPWTVFVNPHWPVMAVHSTIACYAATGYAVAGVYAWGMLRGRGDPLRQKALRIALAMGLIAAVTMPLTGDLSAKVVARHQPEKLAAMEALFVTQARAPLLIGGLPNPEEGTVCCAVAVPGLLSFLAYGRLDAEVAGLDRVPRQEWPNVPLVHISFQLMVGAGIAMVLVGLYYAWVRWRRPEQVPRDRRLLWLLVLSSPLGYLALEAGWIVSETGRQPWTIWKVMLTADAVTPSGSTMALSMAGFTALYAALAAALVLLLNRLRHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX4</accession>
    <name>Q67NX4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase</fullName>
            <ecNumber evidence="3">4.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.-.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1634"/>
    <dbReference type="EnsemblBacteria" id="BAD40619">
        <property type="protein sequence ID" value="BAD40619"/>
        <property type="gene ID" value="STH1634"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1634"/>
    <dbReference type="eggNOG" id="COG2606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094875_3_0_9"/>
    <dbReference type="OMA" id="PYDYVEH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00002">
        <property type="entry name" value="YbaK_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004369">
        <property type="entry name" value="Prolyl-tRNA_editing_YbaK/EbsC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006181">
        <property type="entry name" value="EbsC_YbaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00011">
        <property type="entry name" value="YbaK_EbsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="4">
        <location>
            <begin position="26"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="155" mass="17022" checksum="6191C1B42FD9E503" modified="2004-10-11" version="1">MKTIAARMLDQMKIPYEIRTYEWSEEEMDAVSVARKIGLPPEQVFKTLVVRGDRTGVLVASIPGNAELDLKRLAAVSGNKKVEMVHVRELQGLTGYIRGGVSPLGMKKPYPYYLDETAQIIEQLAVSAGQRGLQLILSGPDLIRATGAKLADIAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P15</accession>
    <name>NUOH1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit H 1</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit H 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit H 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoH1</name>
        <name type="ordered locus">STH1593</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P15"/>
    <dbReference type="SMR" id="Q67P15"/>
    <dbReference type="STRING" id="292459.STH1593"/>
    <dbReference type="EnsemblBacteria" id="BAD40578">
        <property type="protein sequence ID" value="BAD40578"/>
        <property type="gene ID" value="STH1593"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1593"/>
    <dbReference type="eggNOG" id="COG1005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_9"/>
    <dbReference type="OMA" id="MYVGMGI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="PTHR11432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000244958" description="NADH-quinone oxidoreductase subunit H 1">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01350"/>
        </source>
    </evidence>
    <sequence length="328" mass="36524" checksum="C451DEF3B3666C78" modified="2004-10-11" version="1">MNIWMTVLVLVVKAVVLALCVTLAVAVFLLFMRKFLAYFAYRVGPVKVGPWGVLQPIADVIKMLFKEDIMPEQADPLIYRLAPVIAFFCALGVWVVIPWAPRGSWWSSMADPNAGVLVILAIAAMGVYGTALGGWASQNKYGLLGSMRATAQMISYELAMAMSLLGVILMTGSVSMYDIVDAQRNMINLFPQFLGFLVFYIASLAEAGWTPFDLPESETELVAGYHTDYGGMRFGLFFLTELTHAVNSAVLSTTFFLGGYNPWFGLTMIPGFVWFLLKVILMVFVLYWIKCTFPRFRYDRLMVFGWKVLLPVAALNLVGTAIYVALWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P40</accession>
    <name>Q67P40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195697.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1568"/>
    <dbReference type="EnsemblBacteria" id="BAD40553">
        <property type="protein sequence ID" value="BAD40553"/>
        <property type="gene ID" value="STH1568"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1568"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031960_4_1_9"/>
    <dbReference type="OMA" id="AYVINEW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00118">
        <property type="entry name" value="BLACTAMASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="1">
        <location>
            <begin position="34"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="261" mass="28904" checksum="0A7AB490046624C6" modified="2004-10-11" version="1">MPRLRPFRPEDLTAILERVVSDLPGTIGVYVTDLATGRSAGLNPMAEFYAASTIKVPIAMAVLWLADRRRISLDQTIAYQPEDYQAGTGILQATIQPGDEVSVRRLLELTITVSDNIARNMLERFIGSGTIRNYMLSLGVAPAYDPLETPVTPYGMNQVLIALDSGRSGLSPASTRMLLRWMEEAVFRRRIPRYLPESAVVATKIGSRDDEFHDVGLVYAPDRSFAISVFTKGMTEAQAEEAIARIAEAVYWYMDSLTRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P42</accession>
    <name>Q67P42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195695.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1566"/>
    <dbReference type="EnsemblBacteria" id="BAD40551">
        <property type="protein sequence ID" value="BAD40551"/>
        <property type="gene ID" value="STH1566"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1566"/>
    <dbReference type="HOGENOM" id="CLU_118978_0_0_9"/>
    <dbReference type="OMA" id="SHIIFGC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="15580" checksum="F497AE4B036681C6" modified="2004-10-11" version="1">MIPIVFTFLRITIPPFFTATLMSHVPSMLAMLMGPFAAIGVGIGSALGFTIFVGPPIGARALSHALFAWVGNIAWNRGMPLWLVMLIALPVHAVVEAAVVWLLGGNLSMALITLVGTAIHHCVDGGIALGLVAALGRTGVRWFEQPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P47</accession>
    <name>Q67P47_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Stage III sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">spoIIIE</name>
        <name evidence="7" type="ordered locus">STH1561</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1561"/>
    <dbReference type="EnsemblBacteria" id="BAD40546">
        <property type="protein sequence ID" value="BAD40546"/>
        <property type="gene ID" value="STH1561"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1561"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001981_9_7_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="6">
        <location>
            <begin position="569"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="424"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="586"/>
            <end position="593"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="930" mass="96995" checksum="3667810EBA9CDC58" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P92</accession>
    <name>Q67P92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative dinitrogenase reductase activating glycohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1516"/>
    <dbReference type="EnsemblBacteria" id="BAD40501">
        <property type="protein sequence ID" value="BAD40501"/>
        <property type="gene ID" value="STH1516"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1516"/>
    <dbReference type="eggNOG" id="COG1397">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024566_8_2_9"/>
    <dbReference type="OMA" id="PQPRWID"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="SSF101478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="450" mass="47758" checksum="A6A3A07BDFFF7902" modified="2004-10-11" version="1">MDWELRILQALEGSRPGWDEPPDWRAVLALYEGLAPDEAAALDRTVVRMIDLGYRNPHASREPGPFDQIEVSLPAGMAPADLLCIEAAVLVAAERGLGDAYFPFLRLMSVPTWHVIQGRLTWLAREALDAQRRLSLTRAGRGLGALLGLAAADALGCTVEFMSREAIRARWPEGHREIVGGGPFGFPAGDWTDDTAMAVAVGRGILEQPDDPVDAVGRHFVAWFQSGPPDVGNTCRTAINAFLRLGSWEAASAAVRAELGQWAAGNGALMRTLPAALAYGPDPAQAIRIGRMTHPHPESDAAIAVYHRTVDALLEGAAPAEALEAGLAPLPGAEPEVGAAIADLARRLSGLASRSAERIRSGGYVVETLEAALWAFLRTDSLEECVVLAVNLGDDADTVGAVAGGLAGAAYGPGAVPRRWSQALRRRAVIDELAAGLYGVFRARQGDTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P94</accession>
    <name>Q67P94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195644.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1514"/>
    <dbReference type="EnsemblBacteria" id="BAD40499">
        <property type="protein sequence ID" value="BAD40499"/>
        <property type="gene ID" value="STH1514"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1514"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_1_3_9"/>
    <dbReference type="OMA" id="ICCNGAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="29739" checksum="C7EDAE63E55419D9" modified="2004-10-11" version="1">MQYRLIALDVDGTLFCGRHRVSPRTLAALTVARRRGAITVIATGRTPHSALRVSAEIGGGPVICCNGAGLLDEEGRLIHHRSIPTEPLLRVLKLGRQADLLVHGYTPEGVVLDQPMAHLIRAYAWLRAGGAALQSAVGVARLWGANQTRLVPRLTQWAASPHRPPVLKLTLFGDPARLPDVADAIRREVPGVEVTSSGPDNLEVTAAGVSKGSGLAALGERLGIPPEATIAFGDSDNDLTMLRYAGLGVAMGNAPPHVKAAADRVAPPCAEDGVACVLEEVCGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG3</accession>
    <name>Q67PG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195575.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40430">
        <property type="protein sequence ID" value="BAD40430"/>
        <property type="gene ID" value="STH1445"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1445"/>
    <dbReference type="HOGENOM" id="CLU_943108_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="31691" checksum="7195A00AD3276908" modified="2004-10-11" version="1">MGRAAWIVVGWLMIPLGIGLAVVQAVPGEPPGVAGIAPPAAEAQARAAEAQAPATAVLAGEGADAGPSGVELPEEWRRAIDQVVIPREVLDRLPELAAIQQKLMRVDGVLMSWTDHRNGKVFVGVRSLEARERVEAAIAREGIPPEWVEIFGGAGLLSEERPLEGCQLADPPRPEPGPQYLEVTPAVVRPGQGISLVIRGVPEDQLMRGVEAYLECWDGEGWSPRFYLITAYGGGGPHSVLYGGPLVIVDLGLFGTGPERHLVPDQLEPGWYRIRKPLGRTRFVPAEVVGYLQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG7</accession>
    <name>Q67PG7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195571.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1441"/>
    <dbReference type="MEROPS" id="S16.012"/>
    <dbReference type="EnsemblBacteria" id="BAD40426">
        <property type="protein sequence ID" value="BAD40426"/>
        <property type="gene ID" value="STH1441"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1441"/>
    <dbReference type="eggNOG" id="COG3480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042037_2_0_9"/>
    <dbReference type="OMA" id="TDHTFPF"/>
    <dbReference type="OrthoDB" id="1324470at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="129"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="37097" checksum="7D341DDFF0D5C7A0" modified="2004-10-11" version="1">MRAVVLWFRQLDAWVRRLFVLSSLIAVLGAVLVYTPTPYYVTAPGAAIDTSRLVTVENGDVHRGHLYLLVVNTQPANLFWYLYAQVDRRAVLETKEQYLGSFESYAEYLDWNRQLMTESQRTARAVAFQQLGFGEGVRSVGARVVGVLTDSPARGLLHAGDVIVAVAGEPVTGSESLRSRMAGIPGGEEVPVTVLRGGGEVTLTLPTRASQEAGREGSAVFGITIQDELEFDDDAVPVEIRSGSIFGPSAGLMFTLQIIDQLTPGGLTDLVVAGTGTIEPDGRVGRIGGVQQKVYTAEAAGADLMFVPRGNYEEAIAVATRVQVVPVDHVRDALEWLRAAGPQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI6</accession>
    <name>Q67PI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1422"/>
    <dbReference type="EnsemblBacteria" id="BAD40407">
        <property type="protein sequence ID" value="BAD40407"/>
        <property type="gene ID" value="STH1422"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1422"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_9"/>
    <dbReference type="OMA" id="VRQAFYQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269927" evidence="1">
        <location>
            <begin position="24"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="84"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="515" mass="57447" checksum="231FB55EA18FB6F5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ1</accession>
    <name>Q67PJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195547.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1417"/>
    <dbReference type="EnsemblBacteria" id="BAD40402">
        <property type="protein sequence ID" value="BAD40402"/>
        <property type="gene ID" value="STH1417"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1417"/>
    <dbReference type="eggNOG" id="COG2876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062599_0_0_9"/>
    <dbReference type="OMA" id="MEITHES"/>
    <dbReference type="OrthoDB" id="687380at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041071">
        <property type="entry name" value="DAHP_snth_FXD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006268">
        <property type="entry name" value="DAHP_syn_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18152">
        <property type="entry name" value="DAHP_snth_FXD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01361">
        <property type="entry name" value="DAHP_synth_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAHP_snth_FXD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="DAHP_synth_1" evidence="1">
        <location>
            <begin position="93"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="37020" checksum="566F067E18516BA3" modified="2004-10-11" version="1">MIIVMRKGATEAQVNDIVERVRAAGFGVHLSQGEERTIIGAIGGDRAVLADLNLEAAPGVERVTPISKPYKLASRDFHPEDTLIRIGGPTVGGNDFWVVAGPCSVEGRELLLEAARLVRDGGAHALRAGAFKPRTSPYSFQGLGEEGLKYLAEARAVTGLPVVTEVMDTRDVELVAEYADVLQIGTRNMQNFSLLREVGRANRPVLLKRGMSATIEEWLMAAEYVMSEGNHQVILCERGVRTFETATRNTLDLSAVVVAKSLTHLPVVVDPSHGVGRRDFVVALARAAAAAGADGILVEVHPRPDEAKSDAAQTIGPAAFHQMMHDVAAILQVLGRRLPAAAAVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM0</accession>
    <name>Q67PM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1388</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195518.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1388"/>
    <dbReference type="EnsemblBacteria" id="BAD40373">
        <property type="protein sequence ID" value="BAD40373"/>
        <property type="gene ID" value="STH1388"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1388"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050656_0_1_9"/>
    <dbReference type="OMA" id="SLHGWLW"/>
    <dbReference type="OrthoDB" id="1899767at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="21"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="364" mass="37811" checksum="8E6CAAB588F8A238" modified="2004-10-11" version="1">MIQVILAGGLTGILFGFVLQRGRFCMTSGFRDLFLTRDATLFKAYVLALAVQSTGIFVLQAAGIIRLGADPFQWLAAAVGGLIFGISLILSGGCASGTYYRIGEGMVGSLIAAVFFGIGALASRGSGILAPLAQRIQAHTLTVNGATSLHEALGVSPWVLVAGLWVAAILFLVRARRAPAPAWQPPVDESRSWLWRAVFGRGWSWQVTGLAVGLVAVLAWLTSVSTGRMGGLGITGATAGLLRLVVASGDVSALDWSAFLVLGIPVGSFIAARAAGEFRLRAPGPARLLQHIAGGLGMGFGATLARGCNIGNGLVAVSQFSLNGWTATLFIILGTWLGAYLFLVLPARRAESRQVASPGLAHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN5</accession>
    <name>Q67PN5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha 1,2 N-acetylglucosamine transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195503.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1373"/>
    <dbReference type="EnsemblBacteria" id="BAD40358">
        <property type="protein sequence ID" value="BAD40358"/>
        <property type="gene ID" value="STH1373"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1373"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052303_0_0_9"/>
    <dbReference type="OMA" id="IEAMCAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="1">
        <location>
            <begin position="22"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="364" mass="40143" checksum="9CD16472FBE19076" modified="2004-10-11" version="1">MKRVAVLAPAFLGPRGEPPVQGGAERHLIELCRLLEERGYAVTLFQAAAHDFRAVHDGRVVHGLGPTVVQGGAWTVTNRRFRDAARGYDHHLFHLWSMAYPEAPPGSVGVSHSLWWDDGAVVRRPVPNFTPRLREALRNLAVVVANDTNIINWVRATWPEMEGRFRFIPNFVDLAQFAPAPPRTHGGPLRVLVPCRLEPQKGLEPMLEAALMLTEQPGIEVHFVGPGPAEAVRRLQAVAAGRPAIRYRSRVMAEMPAEYRDADVVVIPSVATEGTSLSCLEAMASGRPVVAGWVGGLSNLIIHEYNGLLLRPTAANLAAAVRRLWQDGELRSRMAARAAEVARVHSLERWRRAWGEVLDECWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN7</accession>
    <name>Q67PN7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannose-1-phosphate guanyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1371"/>
    <dbReference type="EnsemblBacteria" id="BAD40356">
        <property type="protein sequence ID" value="BAD40356"/>
        <property type="gene ID" value="STH1371"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1371"/>
    <dbReference type="eggNOG" id="COG1208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="25660" checksum="FC4FD197D96F7509" modified="2004-10-11" version="1">MAGGEGVRLRPYTRILPKPMLPLGHRPILAWLLDRLVAGGVTEVTLAVRYLGYVFRSYFGDGERVGVPVRYVEEAQPMGTAGALRLIPGLDEPFLVVNADIVTGLDFGDFIAFHRSRGGWLTVATQLRRERLNLGVLEVEGERVVAYHEKPERVERLGLGIYAVDPRALACLPDTGPVDMPEWIARLLAEGRPVFQYETDAPWFDLGSPEQYAAVLDRWPQLEERLGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT7</accession>
    <name>Q67PT7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative S-layer associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1321"/>
    <dbReference type="EnsemblBacteria" id="BAD40306">
        <property type="protein sequence ID" value="BAD40306"/>
        <property type="gene ID" value="STH1321"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1321"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_343531_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="622"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="687"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="759"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="824" mass="88200" checksum="11FFAA7E58311CC5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ0</accession>
    <name>Q67PZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1268"/>
    <dbReference type="EnsemblBacteria" id="BAD40253">
        <property type="protein sequence ID" value="BAD40253"/>
        <property type="gene ID" value="STH1268"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1268"/>
    <dbReference type="eggNOG" id="COG1306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_513788_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025275">
        <property type="entry name" value="DUF4015"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13200">
        <property type="entry name" value="DUF4015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4015" evidence="2">
        <location>
            <begin position="78"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="1">
        <location>
            <begin position="435"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="530" mass="56743" checksum="618E8B44EDE57D80" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q24</accession>
    <name>Q67Q24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1234</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195365.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1234"/>
    <dbReference type="EnsemblBacteria" id="BAD40219">
        <property type="protein sequence ID" value="BAD40219"/>
        <property type="gene ID" value="STH1234"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1234"/>
    <dbReference type="eggNOG" id="COG4603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040769_0_1_9"/>
    <dbReference type="OMA" id="YGYLGIM"/>
    <dbReference type="OrthoDB" id="974216at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="357" mass="37657" checksum="4FB179910D52DE4C" modified="2004-10-11" version="1">MPDQSKWKAGLRAVGIPVLATIVGLFVGIVCIVLAGKDPVLAAKAFVRAVAGEPRMFGNVLVSTIPLIFTGLAVAFAYRAGLFNIGVEGQYLMAQIATVVVGVWWTPPKGLEWLHPILALGAGAVAGAIWGGIVGLLKAWKGVHEVINSIMMNWIALFTADWLLKYYLRPDNSRAASYDLQQTAWLKQGLIEGSRLHTGIWIALATALVVWIILYKTPLGYEIRAVGYSPGAAEYGGISVARALIITMAISGAIAGMAGSVQTMGLNHRFNETSALPGYGFDGIAVALVGRLHPAGVVLAALLFGALQASSGLMQAMAGVPKSVVSIVQAVVILFVAAEGVWNFLQKRKAKKGVKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q45</accession>
    <name>Q67Q45_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetamidase/formamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195344.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1213"/>
    <dbReference type="EnsemblBacteria" id="BAD40198">
        <property type="protein sequence ID" value="BAD40198"/>
        <property type="gene ID" value="STH1213"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1213"/>
    <dbReference type="eggNOG" id="COG2421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032013_0_1_9"/>
    <dbReference type="OMA" id="PWGFTGI"/>
    <dbReference type="OrthoDB" id="228571at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004304">
        <property type="entry name" value="FmdA_AmdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31891">
        <property type="entry name" value="PTHR31891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03069">
        <property type="entry name" value="FmdA_AmdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="46159" checksum="486F180079AC1196" modified="2004-10-11" version="1">MGRPLIEIDPTKPISEYYEVIQNRWHPDIPAVASVRPGEVFTVECPDWTGGQVRNDDTATDIRDMELSPNHHLTGPIEVAGAEPGDLLVVDILDIGPHPKQSWGYTGIFCRHNGGGFLTDHFPHAHKAIWEFDGVWARSRHVPGVRIPYLAHPGILGTAPSHDLLARWNERERKLIAQNPERVPPLALPPEPRNAVLGALRPGTPEYERVAREAARTIPPRENGGNRDIKNLTRGARALLPVHVKGAKLTVGDLHYSQGDGEITFCGAIEMPGYITMCVDLIKDGMNKYNLHHAIFQPSLTEPRFSSYIVFEGYSVDEEGRQYYLDAHVAYRMACLEAINYLKSFGYTAEEAYMILGTAPIEGRISSIVDIPNACCTLWLPTEIFEFDIRPRKEGPVRVVSGGRLPTARWEDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q48</accession>
    <name>MURG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="1">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murG</name>
        <name type="ordered locus">STH1210</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195341.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q48"/>
    <dbReference type="SMR" id="Q67Q48"/>
    <dbReference type="STRING" id="292459.STH1210"/>
    <dbReference type="CAZy" id="GT28">
        <property type="family name" value="Glycosyltransferase Family 28"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40195">
        <property type="protein sequence ID" value="BAD40195"/>
        <property type="gene ID" value="STH1210"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1210"/>
    <dbReference type="eggNOG" id="COG0707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037404_0_1_9"/>
    <dbReference type="OMA" id="AADMMLC"/>
    <dbReference type="OrthoDB" id="1165736at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01133">
        <property type="entry name" value="murG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000225105" description="UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <sequence length="374" mass="39993" checksum="4DC838BF7F2C046F" modified="2004-10-11" version="1">MRYLITGGGTGGHIYPALSIARALTEQDPEAELLYVGTRTGREAAIVPQAGIAFAVISSGGVVNLGLLQRVRGGLRAARGLLEALGHIRRFRPDIVIGTGGFVAGPVLAAARLARVPLVIQEQNAFPGVTNRLAARWATAVFVPYEEARAHFPPGVRLIRAGNPVRPEIASASREAGRQALGLSERDRVLVIMGGSGGARDFNRVAAEAVLQLDVPGLRVVHITGERYFGQVKAQYGDRAPHVTLLPYAHNMPEVYAAADAGLFRAGALTLAEIQVRRLPSVLIPSPNVTHNHQEWNARTLERRGAAIVLREGGLTPADLAAALTRLLTDEALADRMRAALGEVADPDAARTIARRIVGIARQSREAREARRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q62</accession>
    <name>Q67Q62_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195327.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1196"/>
    <dbReference type="EnsemblBacteria" id="BAD40181">
        <property type="protein sequence ID" value="BAD40181"/>
        <property type="gene ID" value="STH1196"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1196"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_9"/>
    <dbReference type="OMA" id="WIVDQYT"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="9">
        <location>
            <begin position="182"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="44559" checksum="4F48E2F8A66E7E18" modified="2004-10-11" version="1">MTGQEFFRDTQETIRAAVAKLGHSEGVYKILRNPRRTLEVHIAVTMPDGSVETFLGYRSQHAAVFGPYKGGVRFHPNVTKEEVEALAMLMTLKNAVLGLPYGGAKGGVICDPNALPPTAVEQIARGYVRGLRDMIGPDTDIPAPDVNTNSRVMGWMLDEYLKCTNAIDFSVFTGKSLNLGGIEGRTGATGLGIAYVTREACKVRGVDLKGARVAVQGFGNVGRGAAQALTALGARIVGVTDITGGVYKEDGLDVAALTEYARDRGGVAGFPGAEPLTNEQLFALPVDVLIPAALEGQITGKVAETIQAPIVVEGANGPTTPEGAQVLADRGIMQVPDILANGGGVTVSYFEWVQGQQGGLRWSERTVHRRLDRYMTSAFAAVLAYSERYNVSMREAAYLFAVDRLATGMIERGWVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="144" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB3</accession>
    <name>Q67QB3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="8">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">potA</name>
        <name evidence="10" type="ordered locus">STH1145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195276.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1145"/>
    <dbReference type="EnsemblBacteria" id="BAD40130">
        <property type="protein sequence ID" value="BAD40130"/>
        <property type="gene ID" value="STH1145"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1145"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="RDRQFLG"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015417">
        <property type="term" value="F:ABC-type polyamine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="4"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="350" mass="38850" checksum="3586534D14AEDC79" modified="2004-10-11" version="1">MAHIRFNNVVKRFGDVTVIPGFSATVADGEFLTLLGPSGCGKTTMLRILAGFEKVTDGEVWLGDTLVSSPSVHVPPEKRNIGMVFQSYAVWPHMNVFDNVAYPLRIRGVPRQEMRERVLRALEIVHLQGLENRMPSQLSGGQQQRVALARAIVAEPKMLLLDEPLSNLDAKLREAMRFELKEIQRRLGITVVYVTHDQAEAMAMSDRIIVMNKGVIEQVAPPAEIYDRPKSRFVADFVGLVNFLEATVESADGTIRIGDDLVLKVQLPEGSHQRVLAAVRPEHVRISREGSGVGTVKGTVKRRYYLGDQIDYFVETSGGEVRVTGNPLEDQFNEGDTVVLHIGHAFAFPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF3</accession>
    <name>SYFA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="synonym">pheSA</name>
        <name type="ordered locus">STH1105</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195237.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QF3"/>
    <dbReference type="SMR" id="Q67QF3"/>
    <dbReference type="STRING" id="292459.STH1105"/>
    <dbReference type="PRIDE" id="Q67QF3"/>
    <dbReference type="EnsemblBacteria" id="BAD40090">
        <property type="protein sequence ID" value="BAD40090"/>
        <property type="gene ID" value="STH1105"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1105"/>
    <dbReference type="eggNOG" id="COG0016">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_0_1_9"/>
    <dbReference type="OMA" id="DWHNFTA"/>
    <dbReference type="OrthoDB" id="469058at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000126779" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <sequence length="343" mass="37988" checksum="920E073E177DCE5E" modified="2004-10-11" version="1">MKDQLLKLRDEALAAIAAAADVGAIQEMRVRYLGKKSELSQVLGGLGRLPTVEERKAMGALGNEIKQAITAALDAREAELAAAALQARLASERIDVTLPGAPVRRGHLHILNQVIHRIEEIFIAMGYEVAESRQVETDWYNFEALNIPKGHPARDAQDSLFLSEEVLLRTHTSNTQIRYMLEVARGRTPVKIICPGRVFRRDFEDATHAMMFHQVEGLVIDKGITMASLKGALTEMARALFGPDVGIRLRPSYFPFTEPSAEMDISCIFCGGKGCRTCKGSGWIEIGGSGMVHPNVLRAGGYDPEEVSGWAFGYGPERVAMLMYGIEDIRHFVNNDMRFLRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG9</accession>
    <name>Q67QG9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spore cortex-lytic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1089</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SleB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1089"/>
    <dbReference type="EnsemblBacteria" id="BAD40074">
        <property type="protein sequence ID" value="BAD40074"/>
        <property type="gene ID" value="STH1089"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1089"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053345_0_0_9"/>
    <dbReference type="OMA" id="KTYMAVR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011105">
        <property type="entry name" value="Cell_wall_hydrolase_SleB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042047">
        <property type="entry name" value="SleB_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014224">
        <property type="entry name" value="Spore_cortex_SleB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07486">
        <property type="entry name" value="Hydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02869">
        <property type="entry name" value="spore_SleB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0309">Germination</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0749">Sporulation</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269313" description="Spore cortex-lytic enzyme" evidence="6">
        <location>
            <begin position="26"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="7">
        <location>
            <begin position="37"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_2" evidence="8">
        <location>
            <begin position="125"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24392" checksum="032F375F1875A072" modified="2004-10-11" version="1">MLLILSLIALSAWSLLPRGAEQAEAAARPTLRRGATGDAVREVQQRLRDWGYYEGQVDGRFGPLTEKAVRFFQSKNGLTVDGVVGPETWAALGYSGAQAASAGAQRPGVNIDLLARVVRAEAEAEPYEGKVAVAAVLLNRVADPRFPKTLEGVIYEPHAFESVSNGRIYNTPPTADDLRAARDAVNGWDPTYGAVFFWNPSKPVSSWVWTRQIITQIGNHVFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ6</accession>
    <name>Q67QJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1062"/>
    <dbReference type="EnsemblBacteria" id="BAD40047">
        <property type="protein sequence ID" value="BAD40047"/>
        <property type="gene ID" value="STH1062"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1062"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="DCTKEEF"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28394" checksum="040B9DE04C2460EF" modified="2004-10-11" version="1">MGEPIIVIEGLKKSFGRLEVLRGIDLTIHQGEVVVVIGPSGSGKSTLLRCINFLEKPTAGRVLFHGEEVGQRLVNGRLVPLPEKVLDQQRARMGMVFQRFNLFPHMTALENVIEAPIRVRKLPRHEAEELGLRLLRRVGLADKAGQYPNFLSGGQQQRVAIARALAMEPDVMLFDEPTSALDPELVGEVLQVMQDLANDGMTMVVVTHEMGFAREVGDRVIFMDGGLILEEGPPEQIFGEPRHPRTREFLSKVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK5</accession>
    <name>Q67QK5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195185.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1053"/>
    <dbReference type="EnsemblBacteria" id="BAD40038">
        <property type="protein sequence ID" value="BAD40038"/>
        <property type="gene ID" value="STH1053"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1053"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_0_2_9"/>
    <dbReference type="OrthoDB" id="1374424at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="279" mass="29962" checksum="70A12999F4A06841" modified="2004-10-11" version="1">MKIRLFAFDLDGTLVDRAGRISAENRRAVAMARASGAEVILVTGRSWRTTLPYYRELGLTGPAICYLGALVVEDESGWIGYHRPLAPEAWARLRRFALEEGLSITAVAAADRAVPGFQLPPKDLLVNDVAYSTGPPDDFACWREWNPYTEIDPGLERATAPPTMAAIYGDRAVARVTAAFPDGLPASQFDLSDRVPGETVLHVWHEEVDKGRALARYCRRRGIPASAVMALGDTTVDVPMLRFAGIGVAVPDGHPAALEAADWVATPAEAVARALGAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL2</accession>
    <name>Q67QL2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11 12">Uridine kinase</fullName>
            <ecNumber evidence="7 11 12">2.7.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytidine monophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Uridine monophosphokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">udk</name>
        <name evidence="14" type="ordered locus">STH1046</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + cytidine = ADP + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11 12">
            <text>ATP + uridine = ADP + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 11 12">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11 12">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 11 12">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="7 11 12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195178.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1046"/>
    <dbReference type="EnsemblBacteria" id="BAD40031">
        <property type="protein sequence ID" value="BAD40031"/>
        <property type="gene ID" value="STH1046"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1046"/>
    <dbReference type="eggNOG" id="COG0572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021278_1_2_9"/>
    <dbReference type="OMA" id="TTLKPMH"/>
    <dbReference type="OrthoDB" id="1793376at2"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00551">
        <property type="entry name" value="Uridine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026008">
        <property type="entry name" value="Uridine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRK" evidence="13">
        <location>
            <begin position="7"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="23557" checksum="D85BF68DC9488815" modified="2004-10-11" version="1">MPAPRAVIAIAGGTGSGKTTIANYISAAFPDDVALLPHDAYYKDNRNLTYEERSQLNYDHPDAFDNDLFIAHLKALKAGQAVERPVYNFSTHLREHQTLRVEPAAIIVVEGVLVLAHPELRALYDLSVFVDTDADVRILRRIVRDIHERGRTLDSVINQYLSTVKPMHEAFVEPSKRYANVIIPEGAHNKPGLEVLIAQVRHLIAQHEPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R12</accession>
    <name>Q67R12_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ion-translocating oxidoreductase complex subunit C</fullName>
            <ecNumber evidence="9">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Rnf electron transport complex subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnfC</name>
        <name evidence="12" type="ordered locus">STH896</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfC subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195028.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH896"/>
    <dbReference type="EnsemblBacteria" id="BAD39881">
        <property type="protein sequence ID" value="BAD39881"/>
        <property type="gene ID" value="STH896"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH896"/>
    <dbReference type="eggNOG" id="COG4656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010808_6_0_9"/>
    <dbReference type="OMA" id="QQLYWYS"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00461">
        <property type="entry name" value="RsxC_RnfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010208">
        <property type="entry name" value="Ion_transpt_RnfC/RsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026902">
        <property type="entry name" value="RnfC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43034">
        <property type="entry name" value="PTHR43034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13375">
        <property type="entry name" value="RnfC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01945">
        <property type="entry name" value="rnfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="358"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="400"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="419"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="446" mass="46481" checksum="5600B424005A2894" modified="2004-10-11" version="1">MNLLARFRGGVHPDDRKTTAASATQPLPRPQRVVIPLRQHIGAPCQPLVRKGDQVALGQPIGTAEAPVSAPVHASVSGTVAAVEPWRTPGGRMEQAVVIEPAPEDQPWEGLPDGLGWDLDPESLDPAFIRDRVRQAGLVGMGGAGFPAAVKLTPKPGTEPDVVILNGAECEPAITSDHRLMLEHPEQVVLGLRLFMRASGAKRGIIAVEANKPDAAGKLSQLVAGDPAISVEVLPVRYPQGAEKMLVWALLRRAVPVGGIPLDVGAVVQNVATAAAAAVALAEGRPLTHRIVTVTGRVTRPGNWLVPLGTSFVDLVEASGGLTGPLARVIAGGPMMGIAQPDLDVPVIKTTGSVILLGPEDLDETPAGPCIRCGRCVAACPMGLLPARLCSAALAGDWGAAERYHVRACMECGSCSYVCPARRPLVQSIRLAKYELGRRAAAGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R18</accession>
    <name>Q67R18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH890</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195022.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH890"/>
    <dbReference type="EnsemblBacteria" id="BAD39875">
        <property type="protein sequence ID" value="BAD39875"/>
        <property type="gene ID" value="STH890"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH890"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_3_9"/>
    <dbReference type="OMA" id="VGMYANS"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="28"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="474" mass="49433" checksum="2C58C94E819F6E76" modified="2004-10-11" version="1">MTITSREQKAAPTGEGRAAVNDSYRWLVLIVAGTSAFMGALDGSIINIIIPQIQAQYQATLGDISWVSTAYLVTISSLLLSAGRLGDMWGYRPVFGAGFIVFGLGSLLCGLAPTLPTLIGGRLVQGVGAAVMMALSPALITTTFPGRERGRALGMQATLTFTGLTLGPSLGGFIAGRWGWHWVFLINLPVAAVGAVLAYTQLRPTERRSGQAFDFAGALLFAAGLASVLLALSQAETWGWGDLRTRLLLAGGALLLLLFLWQERRAAQPMLPLWMFRVPAFTGGVAAALLQFSATFVLTFLLPFYLQQFRGLSPSAAGAVMTAQPAAMVAVAGLAGWLSDRIGTRIPATAGMAAIACGLWLVSRAGAATPVTQVALCLALIGLGAGFFNPPNNSSIMSAAPWDRQGVAAALLAAARNVGMVTGITIASTLFAHLSQRMDFLAAFRTTLAVPVGLAVTGALLSLVRPTVAKDADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R22</accession>
    <name>Q67R22_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-dependent DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH886"/>
    <dbReference type="EnsemblBacteria" id="BAD39871">
        <property type="protein sequence ID" value="BAD39871"/>
        <property type="gene ID" value="STH886"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH886"/>
    <dbReference type="eggNOG" id="COG1201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_3_1_9"/>
    <dbReference type="OMA" id="LPFWHGD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="Lhr_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="32"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="255"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1083"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1466" mass="160588" checksum="8527E9DE4A40083E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R47</accession>
    <name>Q67R47_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH861"/>
    <dbReference type="EnsemblBacteria" id="BAD39846">
        <property type="protein sequence ID" value="BAD39846"/>
        <property type="gene ID" value="STH861"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH861"/>
    <dbReference type="HOGENOM" id="CLU_717514_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="385" mass="43238" checksum="0BC8FD5A9C9A4DD1" modified="2004-10-11" version="1">MERFAIPGWDSFLRSSECSLRSSLCSLASLYDRLPPAERVLVDRRAPVLGDRHTPGFHLVSSCQVRLQRIRVRTGSDLGLRMPRGTCVCSRLRLQATRYELPPPAARVLVDRRAPVLGDRNPPVFHLVNMRQVKIQRIRVRTGGDLSLRSPRGTCGRSRLRASASRYELHSPAARVLVDRHPPVPGDRHTPGFHLVSVRQVKIQRIRVRTGGDLSLRSPRGTCGRPRLRASASRYELHSPAARVLVDRHPPVPGDRHTPGFHLVSVRQVKIQRIRVRTGGDLSLRSPRRTCGRPRLRASASRYELHSPAARVLVDRHPPVPGDRHTPGFHLVSVRQVKLQRIRMRTGDDLDRFRRCGPVVAPVCAPARLQVRVSEGPRRAAVAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R56</accession>
    <name>Q67R56_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH852</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglycosylase Slt family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH852"/>
    <dbReference type="EnsemblBacteria" id="BAD39837">
        <property type="protein sequence ID" value="BAD39837"/>
        <property type="gene ID" value="STH852"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH852"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065765_7_0_9"/>
    <dbReference type="OMA" id="YLICAII"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008933">
        <property type="term" value="F:lytic transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000189">
        <property type="entry name" value="Transglyc_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00922">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="2">
        <location>
            <begin position="16"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="168" mass="18993" checksum="AD2C7F5C3BDE7083" modified="2004-10-11" version="1">MKGYPLAYRSEITECARGHGIDPYLIAAVIRAESRFRPEATSPQGARGLMQLMPDTGRWVAAQMGLPYDDAYLYDPVYNIRLGCWYLAALLGEFAGDPVRALAAYNGGMSNVYSWLDTQQWTGERDTLSQIPFSETRHYVANVLRDQRRYRLIYGDWKPERTGGDDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA1</accession>
    <name>Q67RA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoadipate enol-lactone hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194940.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH807"/>
    <dbReference type="ESTHER" id="symth-q67ra1">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39792">
        <property type="protein sequence ID" value="BAD39792"/>
        <property type="gene ID" value="STH807"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH807"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_4_9"/>
    <dbReference type="OMA" id="HAMSVTD"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="267" mass="29263" checksum="D581CE50583759B7" modified="2004-10-11" version="1">MPEIRANGITLHYQWRGQEDGPVLVLINGLLTDLSSWNGHLPHFTPYFRVLTYDCRGQGASEKPDDGPYTPRLHADDLKGLLDGLGVEKAALLGVSSGACVALSFAGRWPERVSALVLANGYGAADTAMQVKLNSWLRAMEAGGGPLRFDVSVAWIWGATFLNRHYEALRPWREKGTALPAHAVLHLIRGAMDHDALVQAANITCPTLLMTGDEDVLTPLRYSRALEQRIPGSRVVVLPEAGHCMFLEQVDLFSRTALDFLRQALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD8</accession>
    <name>Q67RD8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH770</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH770"/>
    <dbReference type="EnsemblBacteria" id="BAD39755">
        <property type="protein sequence ID" value="BAD39755"/>
        <property type="gene ID" value="STH770"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH770"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_9"/>
    <dbReference type="OMA" id="GYEMFAQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="36566" checksum="C7BBAE11095E4F7C" modified="2004-10-11" version="1">MTIRDVARRAGVGVATVSRVLNGTGYVKAETRERVLAAAAELGYVPSQLARGLVRRLSGTVGLVVPDITNPFFPLITRGVEDAASEAGYTVFLCNTDNDPVLEAQDVRKLREHRVDGIIFVGTTERRELVDQLLADDIPVVVMDRQLEHADVDTVTVDNVAGAQAACRHLIELGHRRIAHAAGHQSTRTGQDRCQGYRMALEEADIPYDPACVTWGDFTFESGFRVGQVLLGLSPRPTAVFAGNDLIALGVIRAAEEAGLSVPDDLSVVGFDNIQMAALVRPGLTTVRQPAREMGRLAMTMLLERIRGEFSGPGRRHVYPPELIVRGTTRRREPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD9</accession>
    <name>Q67RD9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH769"/>
    <dbReference type="EnsemblBacteria" id="BAD39754">
        <property type="protein sequence ID" value="BAD39754"/>
        <property type="gene ID" value="STH769"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH769"/>
    <dbReference type="eggNOG" id="COG3039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_2_1_9"/>
    <dbReference type="OMA" id="TSWINIR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13751">
        <property type="entry name" value="DDE_Tnp_1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF772" evidence="1">
        <location>
            <begin position="103"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="404"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="535" mass="61386" checksum="175047F0B55639F8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI8</accession>
    <name>Q67RI8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-oxoadipyl-CoA thiolase</fullName>
            <ecNumber evidence="4">2.3.1.174</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH720</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + succinyl-CoA = 3-oxoadipyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:19481"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57348"/>
            <dbReference type="EC" id="2.3.1.174"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aromatic compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.174" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194853.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH720"/>
    <dbReference type="EnsemblBacteria" id="BAD39705">
        <property type="protein sequence ID" value="BAD39705"/>
        <property type="gene ID" value="STH720"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH720"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_9"/>
    <dbReference type="OMA" id="EPMRPGT"/>
    <dbReference type="OrthoDB" id="550338at2"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033812">
        <property type="term" value="F:3-oxoadipyl-CoA thiolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019619">
        <property type="term" value="P:3,4-dihydroxybenzoate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012793">
        <property type="entry name" value="PcaF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02430">
        <property type="entry name" value="pcaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="8">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="9">
        <location>
            <begin position="278"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="42808" checksum="46A90E2DAA6925A9" modified="2004-10-11" version="1">MAEAVIVDAVRTPIGRYGGALRDVRPDDLAAVVIRALLQRNPAVSPGSIDDVYLGCANQAGEDNRNVARMAALLAGLPVTVPGVTVNRLCGSGLEAVAQAARAVWCGEGEVLVAGGTESMTRAPFVMAKPAVPWARQVEVYDTTLGWRFVNPRLAERYPPISLGETAERVAERWNVTREDQDRFAWQSQMRCAAAVREGRFDAEIVPVPVPGEDGRTELVVRDEHPRPDITLERLARLRPVFRENGTVTAGNASGINDGASALLIMSGKRAAALGLVPMARIVATAVVGVDPSIMGMGPVPATRKALQRAGLTVDQIDLVELNEAFAAQALACIRELGLDPARVNVNGGAIALGHPLGCSGARILTTLVHEMVRRGSRYGLATMCIGVGQGIAMIVEAIRPKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM2</accession>
    <name>Q67RM2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194819.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH686"/>
    <dbReference type="EnsemblBacteria" id="BAD39671">
        <property type="protein sequence ID" value="BAD39671"/>
        <property type="gene ID" value="STH686"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH686"/>
    <dbReference type="eggNOG" id="COG2110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_5_1_9"/>
    <dbReference type="OMA" id="HYGKGLP"/>
    <dbReference type="OrthoDB" id="1812319at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="18757" checksum="098D76E8A7432301" modified="2004-10-11" version="1">MSTWLDGRIEIVTADITTLHVDVIVNAANPSLLGGGGVDGAIHRAAGPQLLEECRTLGGARPGEVKMTKGYRLPAKAVAHAVGPIWRGGNHGEAETLASCYRRALELAEAAGYRTIAFPSISTGAYGYPIEQAARVAQATVKAYLLENDSPLRVTFCCYSPGDTRVYEQVAGEILGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN5</accession>
    <name>Q67RN5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Riboflavin kinase</fullName>
            <ecNumber evidence="5">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">STH673</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CTP + riboflavin = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH673"/>
    <dbReference type="EnsemblBacteria" id="BAD39658">
        <property type="protein sequence ID" value="BAD39658"/>
        <property type="gene ID" value="STH673"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH673"/>
    <dbReference type="eggNOG" id="COG1339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1467499_0_0_9"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="PTHR40706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP-dep_RFKase" evidence="17">
        <location>
            <begin position="35"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAD39658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20015" checksum="C473DC9969F89BFC" modified="2004-10-11" version="1">MQPARALGLTLGGLPASGQGRRNDMGTQIRWTGRVVSGLGVAARWTTLDWFRAAIERLFGFKPVPGTLNAIAEGDRQELDRLLLTAGTVLVPPAEDICCSLVLPAHVARGVRSHPVVLLRPMVYGYNPAQVEFLAPVRLREALELRDGDEIVITIGDNAPAQKWIAADSSRARSDWATSRPSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP8</accession>
    <name>Q67RP8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194793.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH660"/>
    <dbReference type="EnsemblBacteria" id="BAD39645">
        <property type="protein sequence ID" value="BAD39645"/>
        <property type="gene ID" value="STH660"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH660"/>
    <dbReference type="eggNOG" id="COG2312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2541360_0_0_9"/>
    <dbReference type="OrthoDB" id="1068352at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="9256" checksum="A34EF1B9BF692C2B" modified="2004-10-11" version="1">MRRVPGAEDLLNKAFPVVDEEAMAAVAQAIGDAQIVLLGEQNHGDGDAFAAKVRLVEYMHRHHGFDVLLCEACFFSLTRTWER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR8</accession>
    <name>Q67RR8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194773.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH640"/>
    <dbReference type="EnsemblBacteria" id="BAD39625">
        <property type="protein sequence ID" value="BAD39625"/>
        <property type="gene ID" value="STH640"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH640"/>
    <dbReference type="eggNOG" id="COG1434">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107581_2_0_9"/>
    <dbReference type="OMA" id="HWPEVEK"/>
    <dbReference type="OrthoDB" id="1879123at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF218" evidence="1">
        <location>
            <begin position="28"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="187" mass="21555" checksum="421C8F241F2A81B8" modified="2004-10-11" version="1">MSLRLYEGMSLREISEYLFVEDEPTPGDLILVFGGKRLERAERAAELYKAGLAPKVFITGGDKRGIGVCEAERLRDHMVELSVPAEAIDIECEGLNTIEQVQYSVKRIEETIGWKNIKNVLLVSAPHHMRRVKRTIANYIPRETKITCCPDRRTDITRDNWWHTDDGQKLVYRELEKVRTYALHGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW7</accession>
    <name>Q67RW7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2242</name>
        <name evidence="6" type="ordered locus">STH2267</name>
        <name evidence="3" type="ordered locus">STH440</name>
        <name evidence="4" type="ordered locus">STH591</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">IAM14863</strain>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39425">
        <property type="protein sequence ID" value="BAD39425"/>
        <property type="gene ID" value="STH440"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39576">
        <property type="protein sequence ID" value="BAD39576"/>
        <property type="gene ID" value="STH591"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41227">
        <property type="protein sequence ID" value="BAD41227"/>
        <property type="gene ID" value="STH2242"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41252">
        <property type="protein sequence ID" value="BAD41252"/>
        <property type="gene ID" value="STH2267"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2242"/>
    <dbReference type="KEGG" id="sth:STH2267"/>
    <dbReference type="KEGG" id="sth:STH440"/>
    <dbReference type="KEGG" id="sth:STH591"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="299"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="548" mass="61454" checksum="20F8DB57A42DE554" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX3</accession>
    <name>PDRP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pyruvate, phosphate dikinase regulatory protein</fullName>
            <shortName evidence="1">PPDK regulatory protein</shortName>
            <ecNumber evidence="1">2.7.11.32</ecNumber>
            <ecNumber evidence="1">2.7.4.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH585</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the pyruvate, phosphate dikinase (PPDK) by catalyzing its phosphorylation/dephosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ADP + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] = AMP + H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase]</text>
            <dbReference type="Rhea" id="RHEA:43692"/>
            <dbReference type="Rhea" id="RHEA-COMP:10650"/>
            <dbReference type="Rhea" id="RHEA-COMP:10651"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N(tele)-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate = diphosphate + N(tele)-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase]</text>
            <dbReference type="Rhea" id="RHEA:43696"/>
            <dbReference type="Rhea" id="RHEA-COMP:10650"/>
            <dbReference type="Rhea" id="RHEA-COMP:10651"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="EC" id="2.7.4.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PDRP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.32" evidence="1"/>
    <dbReference type="EC" id="2.7.4.27" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RX3"/>
    <dbReference type="SMR" id="Q67RX3"/>
    <dbReference type="STRING" id="292459.STH585"/>
    <dbReference type="PRIDE" id="Q67RX3"/>
    <dbReference type="EnsemblBacteria" id="BAD39570">
        <property type="protein sequence ID" value="BAD39570"/>
        <property type="gene ID" value="STH585"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH585"/>
    <dbReference type="eggNOG" id="COG1806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046206_2_1_9"/>
    <dbReference type="OMA" id="YAQCEFE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00921">
        <property type="entry name" value="PDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005177">
        <property type="entry name" value="Kinase-pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026565">
        <property type="entry name" value="PPDK_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756">
        <property type="entry name" value="PTHR31756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03618">
        <property type="entry name" value="Kinase-PPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000196732" description="Putative pyruvate, phosphate dikinase regulatory protein">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="150"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00921"/>
        </source>
    </evidence>
    <sequence length="273" mass="30839" checksum="FCC87325D841B7E8" modified="2004-10-11" version="1">MVAKPVIFVVSDSVGETADLVTRAALSQWGQRSVEIRRFPMIDDPQSLREVIDAARQQPTLIVYTIIVPEIRKELQRLVDMWDIPAVDIMGPMLDGISRLLGAPEKLEPGRIHRLDDDYFRRIEAVEFAVKYDDGKDPRGFLRADAVLIGVSRSSKTPVSMYLAHRRYKVANLPLVPEVSLPKELFLVPPHKVVGLRVSPEKLHQIREERIKTIGLRSDANYSSMERILQELDYAESVFKKVGCAVIDVTNKAVEETAVRVLEIINRGVNFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY4</accession>
    <name>Q67RY4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="8">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="8">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glySA</name>
        <name evidence="8" type="synonym">glyQ</name>
        <name evidence="10" type="ordered locus">STH574</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH574"/>
    <dbReference type="EnsemblBacteria" id="BAD39559">
        <property type="protein sequence ID" value="BAD39559"/>
        <property type="gene ID" value="STH574"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH574"/>
    <dbReference type="eggNOG" id="COG0752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057066_1_0_9"/>
    <dbReference type="OMA" id="SYYQFQV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="37373" checksum="56A44D9F01B685B6" modified="2004-10-11" version="1">MYFQDLVLTLDKFWAEQGCIIRHPYDVEKGAGTFNPETFFRALGPEPWNVAYIEPCRRPADGRYAQNPNRMQHYFQYQVIMKPSPENIQEIYLEMLERIGIDPKKHDIRFVEDNWEAPSQGAWGLGWEVWCDGMEITQFTYFQQVGGFECKPVSVEITLGLERLASYIQGVDNVWDIMYNEHLRYGDVFERFEWEHSVYNFEQSDTQMLFQLYNMYEAEAKRLLSLDPPLVYPAYDYILKCSHTFNLLDARGAIAVSERQGYILRVRNLARAAAKAYIEMREALGFPLLKPLAERPTWAARYGAGGRGHGTPDQTRTAEGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA7</accession>
    <name>Q67SA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-hydroxyhepta-2,4-diene-1,7-dioate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194585.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH451"/>
    <dbReference type="EnsemblBacteria" id="BAD39436">
        <property type="protein sequence ID" value="BAD39436"/>
        <property type="gene ID" value="STH451"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH451"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_1_9"/>
    <dbReference type="OMA" id="ESEMVIV"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="32022" checksum="B5D25161E1FCAC77" modified="2004-10-11" version="1">MLLVTYRTPGGLALGVRTERGILDVSAAAGRFGRRDVPLHVDQALGMGLDALDPLADLVARAEEEADLFLPEDGLRLGPPVPRPGKILCVGLNYRAHAAEAHMAVPAYPVLFAKFQNAVAGPGDPVVLPSIAEQYDYEAELVVVIGRRARRVPAEAALDYVLGYCNGNDLSARELQMRTSQWLLGKSLDGFLPIGPYLVTRDEVPDPQDLRIRLWRNGEIRQDGHTRNMLFPVAELIAYISRYMTLEPGDIIATGTPEGVIMGLAEKDWLKPGDVVAVEIDGLGRLENRLVAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG6</accession>
    <name>Q67SG6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T2SSF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH392</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH392"/>
    <dbReference type="EnsemblBacteria" id="BAD39377">
        <property type="protein sequence ID" value="BAD39377"/>
        <property type="gene ID" value="STH392"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH392"/>
    <dbReference type="eggNOG" id="COG2064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056917_0_0_9"/>
    <dbReference type="OMA" id="TERHESP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="149"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="289" mass="32192" checksum="13F699DFB377A81A" modified="2004-10-11" version="1">MAMAATWSFLAVLLFGLGLYDLATARRRRLRAMVERAGSGAAAPGADRRLQGWLKARILPRLPLVPGVGSMDELRQLLLWAGRPAGIGAEEFYFLQLVLAFSLALPLLGLHGWTAAALGFLLGLWLPRAWLRRRVSERTRILRRELPRFVHLLATCLEAGLGLTEAVRRVAAESPGLLAGEMLRAVHEMAAGKPAQRAWRDLTERHESPELKEVVSALQQSHRWGVSIAEQLRFTLRTMRERKQQQAQQKAHEASVRMRVPMVALILMPTIVIILGPAVLRLLRHFAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ4</accession>
    <name>Q67SQ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-N-acetylhexosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH304</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.</text>
            <dbReference type="EC" id="3.2.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 20 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.52" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH304"/>
    <dbReference type="EnsemblBacteria" id="BAD39289">
        <property type="protein sequence ID" value="BAD39289"/>
        <property type="gene ID" value="STH304"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH304"/>
    <dbReference type="eggNOG" id="COG3525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020160_1_0_9"/>
    <dbReference type="OMA" id="CATHCNT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102148">
        <property type="term" value="F:N-acetyl-beta-D-galactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015883">
        <property type="entry name" value="Glyco_hydro_20_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22600">
        <property type="entry name" value="PTHR22600"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00728">
        <property type="entry name" value="Glyco_hydro_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="SSF55545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_20b" evidence="6">
        <location>
            <begin position="31"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_20" evidence="5">
        <location>
            <begin position="158"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="645" mass="71967" checksum="61500F1C43C3B2FE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ4</accession>
    <name>Q67SZ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-hydroxyacyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH214"/>
    <dbReference type="EnsemblBacteria" id="BAD39199">
        <property type="protein sequence ID" value="BAD39199"/>
        <property type="gene ID" value="STH214"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH214"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_9"/>
    <dbReference type="OMA" id="HPVRLYD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="2">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="1">
        <location>
            <begin position="184"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH_N" evidence="2">
        <location>
            <begin position="353"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="1">
        <location>
            <begin position="429"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="517" mass="55434" checksum="770737C1411A246D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T07</accession>
    <name>Q67T07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH201"/>
    <dbReference type="EnsemblBacteria" id="BAD39186">
        <property type="protein sequence ID" value="BAD39186"/>
        <property type="gene ID" value="STH201"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH201"/>
    <dbReference type="eggNOG" id="COG5566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159841_0_0_9"/>
    <dbReference type="OMA" id="KAYCLSY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="12258" checksum="38F02F884D66C3A5" modified="2004-10-11" version="1">MLIKTEEAWFRLARYLNAADVLPAELLAEVQRYAAGEQLYIPRPQERLPWGTRTGARAQLALRNEQIRQRRREGASIEELMREFHLSYDSIRKILSGKNGSNCSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T67</accession>
    <name>Q67T67_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell wall-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH141"/>
    <dbReference type="EnsemblBacteria" id="BAD39126">
        <property type="protein sequence ID" value="BAD39126"/>
        <property type="gene ID" value="STH141"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH141"/>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_4_3_9"/>
    <dbReference type="OMA" id="SDPAQWC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269667" evidence="2">
        <location>
            <begin position="35"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="3">
        <location>
            <begin position="289"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="31"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="164"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="390" mass="43647" checksum="09BD75D1355F3B25" modified="2004-10-11" version="1">MPVRMQRRIRWAWVALGALLLNMALPLRPQAAQANDIRAQLEQAQRDLEAVRRKQKEVQNELADLAFQAEEAEAQLRLVEAELVQANANLAIVAAEYEEVSAELELVQAEVALAQARYAEKRKILGDRIRAMRENGPVDKLSVLFGAATFREFISRAEILATIARKDRELVEQMKAEKLALEEKQRQVEGRKTRLAALKAEAEEYQATVVAKREEREQVSRSLEDRRQALAAQLDEMERYGQQLAEQVAALVRELNRQAGVFAPIPPVTPVIITDHFGMRWHPILGTQRMHYGTDFAANYGQTVRAIEAGVVLQTSYDDVYGNLVIIDHGGGITSWYAHNSRILVSPGQAVSQGEAIALAGSTGWSTGPHVHLEIHVDGVQKNPLDYINW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T77</accession>
    <name>Q67T77_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase_C39_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH131"/>
    <dbReference type="EnsemblBacteria" id="BAD39116">
        <property type="protein sequence ID" value="BAD39116"/>
        <property type="gene ID" value="STH131"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH131"/>
    <dbReference type="eggNOG" id="COG3271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054370_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02549">
        <property type="entry name" value="Peptidase_C39A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039564">
        <property type="entry name" value="Peptidase_C39-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039563">
        <property type="entry name" value="Peptidase_C39_single_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13529">
        <property type="entry name" value="Peptidase_C39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_C39_2" evidence="1">
        <location>
            <begin position="147"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="317" mass="35269" checksum="0F7FD0892B378B00" modified="2004-10-11" version="1">MALYPCNAFAAGDHQGTRAGPEGLVGDGTWTAPEQEAAPFDWAVASWNGEGELIEVSLRVRVGDDWSPWFSFGRWSSTGERASVPDQVHPPFGRLETDTLLLDRPARAYQFRVTLRAAALRRLWLAAARKDERSGEPPHRDAWGVELDVPMRSQMVFPDGGNVWCSPVSLAMVMAYYGHVESIPGETVPGVYDAAYRGHGNWPFNTAYASTRGFRAYVDRFGSFAELERWIARRRPLIASVAYDRSWLPNAPIARTAGHILVVRGFTPEGDVIVNDPAAPSDPEVRRVYRRELFRRAWLDRSGVVYVLEPLSAPGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T92</accession>
    <name>Q67T92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH116"/>
    <dbReference type="EnsemblBacteria" id="BAD39101">
        <property type="protein sequence ID" value="BAD39101"/>
        <property type="gene ID" value="STH116"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH116"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3189888_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="46" mass="5236" checksum="9C1E0E02DDA45FBB" modified="2004-10-11" version="1">MTIVEGDARVYLEQQVAKWFQGAELGFEQDPWGGEFTFKHPNMGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD8</accession>
    <name>Q67TD8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Penicillin amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH70</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194205.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH70"/>
    <dbReference type="EnsemblBacteria" id="BAD39055">
        <property type="protein sequence ID" value="BAD39055"/>
        <property type="gene ID" value="STH70"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH70"/>
    <dbReference type="eggNOG" id="COG2366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_0_1_9"/>
    <dbReference type="OMA" id="NFGQSER"/>
    <dbReference type="OrthoDB" id="186419at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023343">
        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218">
        <property type="entry name" value="PTHR34218"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001227">
        <property type="entry name" value="Pen_acylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="740" mass="78604" checksum="3C974A5FFC5A3DD3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF9</accession>
    <name>Q67TF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH49</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH49"/>
    <dbReference type="EnsemblBacteria" id="BAD39034">
        <property type="protein sequence ID" value="BAD39034"/>
        <property type="gene ID" value="STH49"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH49"/>
    <dbReference type="eggNOG" id="COG3415">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038364_3_0_9"/>
    <dbReference type="OMA" id="FDHSFRS"/>
    <dbReference type="OrthoDB" id="1637053at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="166" mass="19212" checksum="72475205A4A15949" modified="2004-10-11" version="1">MDEQQRDEIAAFRWQLIAPVVAEDVTRLERRRILQQIARQHHQIPHSDKTRVSVRTLERWIAAYRQAGFDGLKPKVRDDARKGRVITPEILEHAIAARREVPERSVQQIIDTLEKAGVVPPGLLRRSTLSQHLVRAGYSRKAMLSRMRIPDGFDHSFRSNPSTYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG4</accession>
    <name>Q67TG4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative middle wall protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH44</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH44"/>
    <dbReference type="EnsemblBacteria" id="BAD39029">
        <property type="protein sequence ID" value="BAD39029"/>
        <property type="gene ID" value="STH44"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH44"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_281726_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269979" evidence="2">
        <location>
            <begin position="28"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="21"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="89"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1111" mass="118420" checksum="5A12728929B9FD12" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB1</accession>
    <name>Q67JB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197370.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3257"/>
    <dbReference type="EnsemblBacteria" id="BAD42239">
        <property type="protein sequence ID" value="BAD42239"/>
        <property type="gene ID" value="STH3257"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3257"/>
    <dbReference type="eggNOG" id="COG3870">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143974_1_0_9"/>
    <dbReference type="OMA" id="TFMIGIE"/>
    <dbReference type="OrthoDB" id="2052224at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010375">
        <property type="entry name" value="CdAMP_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38456">
        <property type="entry name" value="PTHR38456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06153">
        <property type="entry name" value="CdAMP_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="109" mass="12067" checksum="4A8573712D3A205C" modified="2004-10-11" version="1">MKLIIAIVQDKDTHRLLSALTQEGFRATKLSSTGGFLREGNTTLLMGVDDEKVEDILRIIKSTCRAREQLVTPLSPMGGPADSYIPYPVEITVGGAIIFVLQVERFEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG4</accession>
    <name>Q67JG4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3204"/>
    <dbReference type="EnsemblBacteria" id="BAD42186">
        <property type="protein sequence ID" value="BAD42186"/>
        <property type="gene ID" value="STH3204"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3204"/>
    <dbReference type="eggNOG" id="COG4587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084465_0_0_9"/>
    <dbReference type="OMA" id="TIVYWYL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="264" mass="29025" checksum="60555A9B0D699FC5" modified="2004-10-11" version="1">MEKGALRRMGAFAAFVAQGFRQQAAYKVEGWLGIVSGLIWFVLFAGIWTALLGNDPVALERQMRYIVAIRILSELGHLPTWEVAEKFRMGDVALELIKPVPLPVRVMGDFLGRSLFRTLRSLPVYVVIWLAFRLTVPDPAALLLFVVSGLLGWVIEASFLLSLTLIALWTIQFNEAENLWFLASSLFSGEFIPLYYLPGWAAALATYLPWAGTYFVPSAILAGTLTGPALWQALALQAAWALAGCGLLAAMWHAGQRKLTVQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI9</accession>
    <name>Q67JI9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BFN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197292.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3179"/>
    <dbReference type="EnsemblBacteria" id="BAD42161">
        <property type="protein sequence ID" value="BAD42161"/>
        <property type="gene ID" value="STH3179"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3179"/>
    <dbReference type="eggNOG" id="COG1259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096111_3_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036104">
        <property type="entry name" value="BFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003729">
        <property type="entry name" value="Bi_nuclease_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02577">
        <property type="entry name" value="DUF151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103256">
        <property type="entry name" value="SSF103256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51658">
        <property type="entry name" value="BFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BFN" evidence="2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17475" checksum="916AF3D731AEA0E6" modified="2004-10-11" version="1">MVSVDLMSVAMIEETATVILVLRAATLKRLLVMEVGLLEGRAITLEVEGIRTPRPLTHELTLRVMEALGATITRVVICDYRDRTFFASLELRTADGRELTIDARPSDALALALRLKVPIMAEEKVLDEAGLDEHELSERQARFQVPESDDEGEPVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK9</accession>
    <name>Q67JK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197272.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3159"/>
    <dbReference type="EnsemblBacteria" id="BAD42141">
        <property type="protein sequence ID" value="BAD42141"/>
        <property type="gene ID" value="STH3159"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3159"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658761_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="50"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="43093" checksum="296842CF3AFA2254" modified="2004-10-11" version="1">MVRRAWWVVLTLAMVLAAQWMMARGHADLAVNEELLAGNPTLHVSAEEEYRGTVIVLHGFGGSKEMMQYWGYALARLGFDVYIPDLPGHGAQTAPMADSPGTANQVRDALVAAGRAEPDRVGLIGHPTSLNPLAPLYDLDAVADAAGRLSSDVGGDVPAGAAPGGSPAWLLLGLAGGVGALPAVAALVRPDPRRSGRRQQPAGLVTGLAAVAVSLLSAVLAVVYLRVPQLGVATLDYLLPYFLVAAVVLLLLRKLWPREFGASAPGEAESVPHSLLRGLAVALAFLGALVPVIHQNVTYFVPTGPRILPLAATALAYWLYAFQEESLKRAVAGGPSPAGLVLGLAAKILMVATWVGAGVLPNPPAHLPAVLPVALGVLVTLEILSGVLAAMRLSALSVALAEAVAVGWTAAVMLPLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW4</accession>
    <name>KAD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">adk</name>
        <name type="ordered locus">STH3054</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197169.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW4"/>
    <dbReference type="SMR" id="Q67JW4"/>
    <dbReference type="STRING" id="292459.STH3054"/>
    <dbReference type="EnsemblBacteria" id="BAD42036">
        <property type="protein sequence ID" value="BAD42036"/>
        <property type="gene ID" value="STH3054"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3054"/>
    <dbReference type="eggNOG" id="COG0563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032354_1_2_9"/>
    <dbReference type="OMA" id="FHNRMRV"/>
    <dbReference type="OrthoDB" id="1491686at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000158867" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="126"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <sequence length="216" mass="23797" checksum="BB51904F077891ED" modified="2004-10-11" version="1">MHIILMGPPGAGKGTQAVLLAGQEGIPHISTGDIFRAHMSQGTPLGKLAKEYVDAGKYVPDDVTNAMVRDRLAQPDCKKGFVLDGYPRTPEQARALDEMLAELGLALDAVINIDVPDELLVERAVGRRVCRSCGATYHVRFNPPREAGRCDRCGGELYQRSDDQEEKVAVRLNLYHSQTAPLLQFYKERGLVRTVRGDQPMDQVTADIKEAVRASR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX1</accession>
    <name>RPOA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit alpha</fullName>
            <shortName evidence="1">RNAP subunit alpha</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoA</name>
        <name type="ordered locus">STH3047</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197162.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JX1"/>
    <dbReference type="SMR" id="Q67JX1"/>
    <dbReference type="STRING" id="292459.STH3047"/>
    <dbReference type="PRIDE" id="Q67JX1"/>
    <dbReference type="EnsemblBacteria" id="BAD42029">
        <property type="protein sequence ID" value="BAD42029"/>
        <property type="gene ID" value="STH3047"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3047"/>
    <dbReference type="eggNOG" id="COG0202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053084_0_1_9"/>
    <dbReference type="OMA" id="LMKFRNF"/>
    <dbReference type="OrthoDB" id="662686at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00059">
        <property type="entry name" value="RNApol_bact_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011773">
        <property type="entry name" value="DNA-dir_RpoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="PTHR32108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02027">
        <property type="entry name" value="rpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000175400" description="DNA-directed RNA polymerase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha N-terminal domain (alpha-NTD)" evidence="1">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha C-terminal domain (alpha-CTD)" evidence="1">
        <location>
            <begin position="245"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00059"/>
        </source>
    </evidence>
    <sequence length="315" mass="35030" checksum="D3A7DB0DEAFB1E4E" modified="2004-10-11" version="1">MIGMEKPKIETVVLAEDNSYGKFVVEPLERGYGITLGNSLRRILLSSLPGAAVTSVKIDGVLHEFSTLPGVVEDVTDIILNLKQLSLRMHSDEPKVLRLHAEGEGEVTAGDIHTDADVEILNPDLHIATLDKGGRLIAEITVSKGRGYVPADQNKTPDMPIGVIPVDSIFSPIRRVNYTIEHTRVGNKTNYDKLTLEVWTNGAIRPDEACSWAAKILKEHLELFISLTEDADEIEVMQEKEDDERNKLMEMTIEELDLSVRSYNCLKRAGINTIAELVSKTDEEMMKVRNLGKKSLEEVKTKLAAFGLSLRQPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K02</accession>
    <name>Q67K02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197130.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3015"/>
    <dbReference type="EnsemblBacteria" id="BAD41998">
        <property type="protein sequence ID" value="BAD41998"/>
        <property type="gene ID" value="STH3015"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3015"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_2_9"/>
    <dbReference type="OMA" id="GRQMFFG"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013570">
        <property type="entry name" value="Tscrpt_reg_YsiA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08359">
        <property type="entry name" value="TetR_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="212" mass="23271" checksum="DA9F217562AF3C8C" modified="2004-10-11" version="1">MSVRGGDKYAAILDAAQHVIARQGYHNAQISRIAAEAGVAAGTVYLYFQNKPDLLVCLFRERLNLLVSEIKEAIGDAVDPREKLRRAIAQHLRSLGTRPELAAVTQIEMRQADTQVQQQISNLMKGWFAVIDQIIAEGQASGLFTREVPAKQIRNLIFGTLDQTVTAWVMSGYKFDILALAEPTYRLIAHGVMQHREGGVGNGDSGSPEADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K15</accession>
    <name>Q67K15_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fliG</name>
        <name evidence="13" type="ordered locus">STH3002</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197117.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3002"/>
    <dbReference type="EnsemblBacteria" id="BAD41985">
        <property type="protein sequence ID" value="BAD41985"/>
        <property type="gene ID" value="STH3002"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3002"/>
    <dbReference type="eggNOG" id="COG1536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047835_1_1_9"/>
    <dbReference type="OMA" id="QVRPFEF"/>
    <dbReference type="OrthoDB" id="847822at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000090">
        <property type="entry name" value="Flg_Motor_Flig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023087">
        <property type="entry name" value="Flg_Motor_Flig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011002">
        <property type="entry name" value="FliG_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032779">
        <property type="entry name" value="FliG_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028263">
        <property type="entry name" value="FliG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30534">
        <property type="entry name" value="PTHR30534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01706">
        <property type="entry name" value="FliG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14841">
        <property type="entry name" value="FliG_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14842">
        <property type="entry name" value="FliG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003161">
        <property type="entry name" value="FliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00954">
        <property type="entry name" value="FLGMOTORFLIG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48029">
        <property type="entry name" value="SSF48029"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00207">
        <property type="entry name" value="fliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliG_N" evidence="12">
        <location>
            <begin position="7"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_M" evidence="11">
        <location>
            <begin position="119"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_C" evidence="10">
        <location>
            <begin position="222"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD41985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="338" mass="38174" checksum="5CE0E2A1AD98AABA" modified="2004-10-11" version="1">MADRDQKLTSRQKAAIFCINIGVERSAKILKHLRDDEIEMLTVEIAQSRKVTPEVRDQVLTEFIMVATADKHIRTGGIDYAREMLEKALGQERARDILTRLTSHLRRRPFDAVRHTDPVHLASFLQNEHPQTVAVIMAHLDADKAAQVLLGLPPERQADIIRRVATLDRTSPEMLREVEKVLERKASAIVSQESTAAGGINWAVDVLNRVDRTTEKTIMGLLSADDPDLASEIAQRMFLFDDIVNLDDRTVQRILREVDMNKDLPLALKAAKEEVWRKIMNNVSKRAGEALREAVEYLGPVRIRDVEEAQARIVALIRRLEESGEIQIARGGGADEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K36</accession>
    <name>Q67K36_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">flgL</name>
        <name evidence="6" type="ordered locus">STH2979</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2979"/>
    <dbReference type="EnsemblBacteria" id="BAD41962">
        <property type="protein sequence ID" value="BAD41962"/>
        <property type="gene ID" value="STH2979"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2979"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024437_5_0_9"/>
    <dbReference type="OMA" id="MTEIGGR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="7"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="208"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="32737" checksum="A1DA59B7BDE38E1C" modified="2004-10-11" version="1">MRVTNWMLVNTAIRELEGLRAQYARAQKRVYGRALERPSDDPQRVIEAIDLTGMKVKLERAQRAATDAKEWLSIAETSLTAMSEDLQAVYETMIQFGSPANQESTARENLALQIEGLRDAIKREMNTQHRGRYVFAGWAADTQPFEDDGSGGVEYKGSKHKIEREIAPGYKVAINIHGDELPYEIIATLTEVANAIRTGTVEDVTKHLDKVQAAADELLARRSEVGLAYQQVEQFEGYSRDGLITVEERLGKISGGDIAEAVMEMTQAQQAFQVALTAFSMALPKSLMDYMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K45</accession>
    <name>Q67K45_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197085.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2970"/>
    <dbReference type="EnsemblBacteria" id="BAD41953">
        <property type="protein sequence ID" value="BAD41953"/>
        <property type="gene ID" value="STH2970"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2970"/>
    <dbReference type="HOGENOM" id="CLU_2345623_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="97" mass="10292" checksum="BF890F0B22EE0B3A" modified="2004-10-11" version="1">MDKLIGIILVLVGLAALFRITLSGAWLFLLIAAALALAVKSGWLGRGAYVAAVVFVLLAVAGLAARTVIFGLAMLFRLAPLILLALGIYYVYKAFVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K79</accession>
    <name>ALR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine racemase</fullName>
            <ecNumber evidence="1">5.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">alr</name>
        <name type="ordered locus">STH2936</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-alanine = D-alanine</text>
            <dbReference type="Rhea" id="RHEA:20249"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="5.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alanine racemase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197052.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67K79"/>
    <dbReference type="SMR" id="Q67K79"/>
    <dbReference type="STRING" id="292459.STH2936"/>
    <dbReference type="EnsemblBacteria" id="BAD41919">
        <property type="protein sequence ID" value="BAD41919"/>
        <property type="gene ID" value="STH2936"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2936"/>
    <dbReference type="eggNOG" id="COG0787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028393_2_2_9"/>
    <dbReference type="OMA" id="HMTHFSD"/>
    <dbReference type="OrthoDB" id="859043at2"/>
    <dbReference type="UniPathway" id="UPA00042">
        <property type="reaction ID" value="UER00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000066057" description="Alanine racemase">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for D-alanine" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for L-alanine" evidence="1">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01201"/>
        </source>
    </evidence>
    <sequence length="377" mass="40523" checksum="A4CF77318790CDFC" modified="2004-10-11" version="1">MDLDGMRPTWAEVDLNAVRANIRALKRISKAPRLMAVVKANGYGHGAVPVATAAIEAGADWLGVASVEEGVTLRRHGISAPILVLGYVSPGQAEAVLTEGLRVALFDGELGQALNREGRRLGRWARVHLKVDTGMGRIGLQPAEVGRLGRELARLDHVEVEGVFTHLATADEPGNPYTRLQLERYEAALAELAAAGVRPAIRHAANSAGLMLHPEAHYDMVRSGIAVVGLPPAPGVAWPVKLAPALTWKTRVGLVKWLEAGHSISYGCTYTTARREQIATLPVGYADGYPRRLSNRAQVLIRGRRCPVVGVVTMDQMMVRVPDDLPVRVGDEVVLIGRQGGEEITATELAGLADTISYEIVCGISRRVPRFYGGETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K87</accession>
    <name>Q67K87_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosomal protein S1-like RNA-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2928</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197044.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2928"/>
    <dbReference type="EnsemblBacteria" id="BAD41911">
        <property type="protein sequence ID" value="BAD41911"/>
        <property type="gene ID" value="STH2928"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2928"/>
    <dbReference type="eggNOG" id="COG2183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009833_0_2_9"/>
    <dbReference type="OMA" id="RWAWRTR"/>
    <dbReference type="OrthoDB" id="120764at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05685">
        <property type="entry name" value="S1_Tex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044146">
        <property type="entry name" value="S1_Tex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018974">
        <property type="entry name" value="Tex-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032639">
        <property type="entry name" value="Tex_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09371">
        <property type="entry name" value="Tex_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16921">
        <property type="entry name" value="Tex_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="651"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="719"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="764" mass="84986" checksum="F2B09BCDB7C924AA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K94</accession>
    <name>TSAD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsaD</name>
        <name type="synonym">gcp</name>
        <name type="ordered locus">STH2921</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197038.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67K94"/>
    <dbReference type="SMR" id="Q67K94"/>
    <dbReference type="STRING" id="292459.STH2921"/>
    <dbReference type="EnsemblBacteria" id="BAD41904">
        <property type="protein sequence ID" value="BAD41904"/>
        <property type="gene ID" value="STH2921"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2921"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_0_2_9"/>
    <dbReference type="OMA" id="HLEGHIY"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303581" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <sequence length="341" mass="35728" checksum="7A1B8FD0201E7754" modified="2004-10-11" version="1">MKGLSEGLILGIESSCDETAAAVVAGGRRVLSNTVASQVDLFRKYGGVVPEIASRRHLELILPVIREALQMAGVTLEQIDAVAVTHGPGLVGTLLVGLSAAKALAFGLDKPLIGVNHLQGHIYANFLVEPPPEFPLVCLVVSGGHTDLIYMAGHGRMELLGRTLDDAAGEAFDKVARSVGLGYPGGPQVEKLARSGDPEAVPLPRAHTEGPYDFSFSGLKTAVLQYVQRTGPLNDQQRADLAASFQRAVTSVLVERTVKAAEAKGVRQVILAGGVAANGALREEMRAALEPRGIRLGYPPPVLCTDNAAMIAAAGYYLWRAGVRHDLDLNAVPGLGLGIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA4</accession>
    <name>Q67KA4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197028.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2911"/>
    <dbReference type="EnsemblBacteria" id="BAD41894">
        <property type="protein sequence ID" value="BAD41894"/>
        <property type="gene ID" value="STH2911"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2911"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_10_9"/>
    <dbReference type="OMA" id="EMEVWFA"/>
    <dbReference type="OrthoDB" id="1745184at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039538">
        <property type="entry name" value="BetI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13977">
        <property type="entry name" value="TetR_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="23130" checksum="A22CC06391B1B8D8" modified="2004-10-11" version="1">MPKIVDHEEQRKRIAEAMWRVILRDGMEGATVRKIAAEAGMSVGALRHYFRQQDDLLVYAMRLVNERVEQRILRLARTNLPPLEKAVRLLLEVVPVDGERRAEMDVWFSFMEHVRHRPDLEGIPENRLHLFVSQVLAYLQHEGLLEEGLDLALEAERMYAIVDGLAVHALLEPERLRPETMEEVVRTSLRRICRNRPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG3</accession>
    <name>PUR9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional purine biosynthesis protein PurH</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Phosphoribosylaminoimidazolecarboxamide formyltransferase</fullName>
                <ecNumber evidence="1">2.1.2.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">AICAR transformylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">IMP cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.10</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">ATIC</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">IMP synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Inosinicase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">purH</name>
        <name type="ordered locus">STH2850</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The IMP cyclohydrolase activity resides in the N-terminal region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.3" evidence="1"/>
    <dbReference type="EC" id="3.5.4.10" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KG3"/>
    <dbReference type="SMR" id="Q67KG3"/>
    <dbReference type="STRING" id="292459.STH2850"/>
    <dbReference type="EnsemblBacteria" id="BAD41835">
        <property type="protein sequence ID" value="BAD41835"/>
        <property type="gene ID" value="STH2850"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2850"/>
    <dbReference type="eggNOG" id="COG0138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016316_5_2_9"/>
    <dbReference type="OMA" id="WRVAKFV"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000018976" description="Bifunctional purine biosynthesis protein PurH">
        <location>
            <begin position="1"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="540" mass="57507" checksum="B01DBC29E903F631" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG6</accession>
    <name>Q67KG6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196966.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2847"/>
    <dbReference type="ESTHER" id="symth-q67kg6">
        <property type="family name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41832">
        <property type="protein sequence ID" value="BAD41832"/>
        <property type="gene ID" value="STH2847"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2847"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808713_0_0_9"/>
    <dbReference type="OMA" id="KPPILWI"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="342" mass="36910" checksum="C74531787C82DC3D" modified="2004-10-11" version="1">MTSRLRIHVREAGSGPVPVVLLHGNCSSSAFFDELMLSLGPRYRAIAPDLRGYGQTEPLPVDATRGCGDWADDLAALVDALGLGRFHLAGWSMGGGIAMRYAPDHADRLLSLTLIAPLSPFGFGGTRDAEGTPTAPDFAGSGGGTVNRDFVGRIAEGDRGADHPSSPRNILNSFYFKPPFRGTEEQEERWVDALLSTRIGDDHYPGDAVPSGNWPDMAPGTRGINNAMSPKYHNVSHFAHIDPKPPVLWIRGDSDQIVSDTSLFDLAYLGQIGAVPGWPGPEACPPQPMVAQTRFVLERYRAGGGEYEEVVIADAGHSPFIEQPQAFLSHFLRHLERSSAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ6</accession>
    <name>Q67KJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2817"/>
    <dbReference type="EnsemblBacteria" id="BAD41802">
        <property type="protein sequence ID" value="BAD41802"/>
        <property type="gene ID" value="STH2817"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2817"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038143_0_0_9"/>
    <dbReference type="OMA" id="MANGQCF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="39204" checksum="E57C1EF0834E5A51" modified="2004-10-11" version="1">MPPNASLGPNPPPRVSAIVPACNEARSIESAVRSLIRQDYPNLEVVLVNDRSTDGTGAIMDRLAREFPQVTVVHIDRLPAGWLGKNHALWVGARHASGEILLFTDADVHYDPTTVRRAVAFMEQRRLDHLTLAPDLTVRGYWLEAWVGFFVMAFLAYKTPYRANDPRSKVGTGIGAFNMIRRTAYEAIGTHRAISLRPDDDLRLGQRLKRMGHSSHVAMGRGLVSVEWYTSLREAIRGLEKNAFAGLEYNLGTVFASVVGILAIMVWPYVALFLTEGWTLGLYAGAILLQLALYVLANGAGGPRMCQLALAYPVAALLFAYAIARATYLTLRRGGIAWRGTFYPLALLRSQSGLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ9</accession>
    <name>Q67KJ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2814"/>
    <dbReference type="EnsemblBacteria" id="BAD41799">
        <property type="protein sequence ID" value="BAD41799"/>
        <property type="gene ID" value="STH2814"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2814"/>
    <dbReference type="HOGENOM" id="CLU_2036868_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="13423" checksum="EDD80363FC55FB32" modified="2004-10-11" version="1">MTKKGMSWGTSGAHHMAQIRSLEAEGQLKHWLGGWQKRRWPEAKAVDRRHGPCRVIERLATTDPAGWLAARLPLLASAAGNSELGRRLKRLAQAVPTSELISRGQPAPWRSRMIPTRQASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU7</accession>
    <name>Q67KU7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transposase</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2288</name>
        <name evidence="4" type="ordered locus">STH2714</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">IAM14863</strain>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41273">
        <property type="protein sequence ID" value="BAD41273"/>
        <property type="gene ID" value="STH2288"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41699">
        <property type="protein sequence ID" value="BAD41699"/>
        <property type="gene ID" value="STH2714"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2288"/>
    <dbReference type="KEGG" id="sth:STH2714"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="306"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="562" mass="63506" checksum="7ED53D8B32E62586" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L38</accession>
    <name>Q67L38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2623</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2623"/>
    <dbReference type="EnsemblBacteria" id="BAD41608">
        <property type="protein sequence ID" value="BAD41608"/>
        <property type="gene ID" value="STH2623"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2623"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_2_9"/>
    <dbReference type="OMA" id="IFATYSI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269253" evidence="3">
        <location>
            <begin position="28"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="62"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="PBPe" evidence="5">
        <location>
            <begin position="62"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="291" mass="31106" checksum="FFADD5D3CECE5E4B" modified="2004-10-11" version="1">MMMKKTRTLALGLALSLLLAACGGVQGGGTPSGGAQQNPPSQSDQQAEAPVSRLEQIKQAGKIVVATSADYPPFEYHAIIDGKDKIVGWEMELAEAIANELGVKLEITEGIFDTLLLDLAAGKADMVISAMTITPERLESVDFSEPYWMTAQSVLIPKAAEGTIQSAEDLIGKTIGVQLGTTGEIAAKEVEGAEVRSYELFEAAVLDLVAGRVQAVVGDYAVVKNYAATRPELTVAFELTKEENAVAFRQDDDELREAVNAILAKLKEDGTIDQLIEKYEVAPPPEVKAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L64</accession>
    <name>Q67L64_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate dehydrogenase associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2597"/>
    <dbReference type="EnsemblBacteria" id="BAD41582">
        <property type="protein sequence ID" value="BAD41582"/>
        <property type="gene ID" value="STH2597"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2597"/>
    <dbReference type="eggNOG" id="COG1526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_2_0_9"/>
    <dbReference type="OMA" id="RYCAGAT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="26862" checksum="7B5B6F947D35445E" modified="2004-10-11" version="1">MTDFGELPPMPVERPATLYINDQEIVTLQTTPSHLDDWAVGFLFGEGIIRDLGEVARLSVDEDRGMVWVDLPGLTELPDASTRKRYLTAGCGKGVTFSSLKDALLLKKVDHPLTVRVADLLDWVKEMQRNTPLYAATGGVHGAGLKNLATGEMIVREDIGRHNALDKAIGAALRAGWDFTRVVAVSSGRISYEAATKLGRAQIAVGATLTAATDQAVRLATHLGMDLVGYARSPKHMVLYTRSGRLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L71</accession>
    <name>Q67L71_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Selenide, water dikinase</fullName>
            <ecNumber evidence="5">2.7.9.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Selenium donor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Selenophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">selD</name>
        <name evidence="8" type="ordered locus">STH2590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + hydrogenselenide = AMP + 2 H(+) + phosphate + selenophosphate</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the selenophosphate synthase 1 family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2590"/>
    <dbReference type="EnsemblBacteria" id="BAD41575">
        <property type="protein sequence ID" value="BAD41575"/>
        <property type="gene ID" value="STH2590"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2590"/>
    <dbReference type="eggNOG" id="COG0709">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032859_0_1_9"/>
    <dbReference type="OMA" id="DNEPKYG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00625">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023061">
        <property type="entry name" value="SelD_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="PTHR10256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0711">Selenium</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AIRS" evidence="6">
        <location>
            <begin position="23"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="7">
        <location>
            <begin position="141"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="33420" checksum="5BAE2188FDD9EE19" modified="2004-10-11" version="1">MLRHLPPSLTNADVIVGLDTPDDAGVYRITDDLALVQTADFFTPIVDDPYWFGAIAAANSLSDIYAMGGRPVTALNLVSFPVSKLDKRILAEILRGGAEKVAEAGAVLIGGHSIDGPEPTYGLAVTGLVHPDRVGAKAGVRPGDRLILTKPIGVGVITTAIKRGLARPADVDEVTQVMAKLNNVVDVLAPFDIRGMTDITGFGLLGHASEMARTSGVGLRLSAGAVPVLPSTYDYAEQGAFPGGSKANFRFLTGEGHVEFAESVPELVRMILCDAVTSGGLLIAVEAARAADLASALTAHGTLAAAVIGEAVAEHPGKIMVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L88</accession>
    <name>Q67L88_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196695.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41558">
        <property type="protein sequence ID" value="BAD41558"/>
        <property type="gene ID" value="STH2573"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2573"/>
    <dbReference type="HOGENOM" id="CLU_2588426_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="81" mass="8127" checksum="3BFC336C0764CB5A" modified="2004-10-11" version="1">MHYGGFGKIPAFSPFIGAQAGADDLFIAPGAAAYPAANATVYRVYEYAVPVTTVATVPSAYPTTAGIPGIAAYPAGKGFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L94</accession>
    <name>Q67L94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41552">
        <property type="protein sequence ID" value="BAD41552"/>
        <property type="gene ID" value="STH2567"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2567"/>
    <dbReference type="HOGENOM" id="CLU_3174057_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="47" mass="5153" checksum="7028FB5E7B893EC2" modified="2004-10-11" version="1">MPHGDLEDFHQGHLKKGFQNLPKGLPNFPYSTGPTSAPMLCRGLTLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF9</accession>
    <name>Q67LF9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="8">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2502"/>
    <dbReference type="EnsemblBacteria" id="BAD41487">
        <property type="protein sequence ID" value="BAD41487"/>
        <property type="gene ID" value="STH2502"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2502"/>
    <dbReference type="eggNOG" id="COG0685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057297_0_0_9"/>
    <dbReference type="OMA" id="QGVHLMA"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006555">
        <property type="term" value="P:methionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0274">FAD</keyword>
    <keyword evidence="5 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="326" mass="35360" checksum="5F893F4A6CFD397D" modified="2004-10-11" version="1">MRSSGLSANPSFSKGSLRMEGRGGSAMARLMHRLQQGAFCVTVEIDPPRGAGVAGTLERVRRFGDRVDAVNVADCPMANVRMSPITLAHLLQRDGQVEAIFHLTTRDRNIIGLQAELLGAAGLGVRNILALRGDDPSRGDHPDARGVFEVDTIGLIRLAAALNRGQTVSGTPLEQGTDFAIGCACNPAAADLEAEVSRLEEKVEAGAHFAQTQPVYDPRQVERFQAKLAGRVKIPILYGVLPLKSYEFADRLNRTVPGIVIPGWVLDRMRGRDEEEGLRLTAELLREIGPHVHGVHIFPMNSARRVVAVLDLIDELGLRHSVRVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK6</accession>
    <name>Q67LK6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2455"/>
    <dbReference type="EnsemblBacteria" id="BAD41440">
        <property type="protein sequence ID" value="BAD41440"/>
        <property type="gene ID" value="STH2455"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2455"/>
    <dbReference type="HOGENOM" id="CLU_3048733_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="54" mass="6089" checksum="01902C3D713A462C" modified="2004-10-11" version="1">MKVVVVLGTLLVAVYTLNYARWAWRRQLRFGAAGLVLLAVATVAVPAWIMWFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU0</accession>
    <name>Q67LU0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2371"/>
    <dbReference type="EnsemblBacteria" id="BAD41356">
        <property type="protein sequence ID" value="BAD41356"/>
        <property type="gene ID" value="STH2371"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2371"/>
    <dbReference type="HOGENOM" id="CLU_2398517_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="10832" checksum="B6BEFDBBDB712902" modified="2004-10-11" version="1">MREVLTELQRLLQAEGLDGQYGYLLRRCFELLSWAGNEPDAAAALKEYLLGIPNAPGTLWDLVIWRDSFEERKRVNERLDQLRERLMALARSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX4</accession>
    <name>Q67LX4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribose 5-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196460.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2337"/>
    <dbReference type="EnsemblBacteria" id="BAD41322">
        <property type="protein sequence ID" value="BAD41322"/>
        <property type="gene ID" value="STH2337"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2337"/>
    <dbReference type="eggNOG" id="COG0698">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091396_4_1_9"/>
    <dbReference type="OMA" id="QKMEFKK"/>
    <dbReference type="OrthoDB" id="1346802at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016861">
        <property type="term" value="F:intramolecular oxidoreductase activity, interconverting aldoses and ketoses"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004785">
        <property type="entry name" value="RpiB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="SSF89623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01120">
        <property type="entry name" value="rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16665" checksum="8914D98E993B1D1A" modified="2004-10-11" version="1">MRIAIGNDHVGTEMKRAIAAHLESLGHEVVNFGTDSTERTDYPIYGERVARAVAAGEVDCGILICGTGVGISLAANKVRGIRAVVCSEPYTARLSKQHNNTNILAFGARVVGVDLAKMIVDEWLNASFEGGRHQRRVDMIADIERREECGPEGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M02</accession>
    <name>Q67M02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative PTS system, IIB component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196432.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2309"/>
    <dbReference type="EnsemblBacteria" id="BAD41294">
        <property type="protein sequence ID" value="BAD41294"/>
        <property type="gene ID" value="STH2309"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2309"/>
    <dbReference type="eggNOG" id="COG3414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159248_0_1_9"/>
    <dbReference type="OMA" id="SMKITVV"/>
    <dbReference type="OrthoDB" id="1884107at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013011">
        <property type="entry name" value="PTS_EIIB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003501">
        <property type="entry name" value="PTS_EIIB_2/3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02302">
        <property type="entry name" value="PTS_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51099">
        <property type="entry name" value="PTS_EIIB_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIB type-2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="9392" checksum="1A57A21BC0F2A2A1" modified="2004-10-11" version="1">MKILAVCGMGLGSSLMLRMQVESALKELGVTGVSVEVADVGTAGGAGADIIVTSPQFAPMLTNAAAKVVAIQNYMDRKEVTQKIKTALDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M30</accession>
    <name>Q67M30_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2280"/>
    <dbReference type="EnsemblBacteria" id="BAD41265">
        <property type="protein sequence ID" value="BAD41265"/>
        <property type="gene ID" value="STH2280"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2280"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1119705_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="64"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="27135" checksum="5564B7CF426F8245" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWERIREELLRGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRGVVPEIVEIQSGGPQAPFGITGVDSRKEVAATDVIVPREDQEAPVAGGGQTALVAPAGAGDAACPHGSSQGGSAGPGRRCGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M31</accession>
    <name>Q67M31_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2279</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2279"/>
    <dbReference type="EnsemblBacteria" id="BAD41264">
        <property type="protein sequence ID" value="BAD41264"/>
        <property type="gene ID" value="STH2279"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2279"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_2_2_9"/>
    <dbReference type="OMA" id="QQMMATE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="47750" checksum="2A42584C1314F750" modified="2004-10-11" version="1">MPPLMSLYRGKTKKRQLLEVDKQPWLPQQVQVTLHALMGAAKEGLLALAVAVGLDVLRSMMEAEVTAIVGPKGKHNPNRKAFRHGAEEGRVVLGGRKVPIQRPRVRTKDGQEVRLSSYEAFQDEQLLTQAALERMLHGLSTRRYHHGLEPVGDDLPAVATSKSSVSRHFVAATRKALAELLARPLGDQRYLVLMLDGIEVADHTVVVALGITDDGQKQILGLWEGATENATVCRALLTDLVERGLRVDGGILVVIDGAKALRAAVRDVLGARATVQRCQVHKKRNVLDHLPDEARAWVSRKLEQAWRETDYEKARTALTSLAKTLDDKYPGAAASLREGLEETLTVLRLELPEALRKTLRSTNPIESAFEKVRMASRNVKRWRNGQQVLRWTAAGLLEAEKGFRRIKGYRQLPMLSEALSRACCEIPVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M49</accession>
    <name>Q67M49_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2261"/>
    <dbReference type="REBASE" id="15232">
        <property type="entry name" value="M.StpORF2261P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41246">
        <property type="protein sequence ID" value="BAD41246"/>
        <property type="gene ID" value="STH2261"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2261"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027633_2_1_9"/>
    <dbReference type="OMA" id="HEISYRA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0680">Restriction system</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="5">
        <location>
            <begin position="46"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="44172" checksum="05C7F438F793C9FC" modified="2004-10-11" version="1">MFAGISPSPAELSHYRKPTSFAIDVTPEAATMTTAPHLRLNALCPYFTMFPLDFPTRYLAQARPGEWVLDPFCGRGTTNYAARLHGLPTVGIDSSPVAVAIARAKLVQVTPDELIAEAERILAGPEPADVPEGHFWDLAYHPRTLLDICRLREALLRDCEAPVRIALRGLILGILHGPRTRRAPSYLSNQMPRTYATKPDPAVRFWVRRGMRPEEVDVLDVLARRARYSFAALPPPVPGCILQADSREPGAIPVVDGGYRWVITSPPYLGMRSYQPDQWLRLWFLGGEPTVRYIHEGQVSHLQGEYQRDLATVWRNVAARCRPGTRLIIRFGCLPTLPCNPKQVLEETLARSGVPWRIVEVTGAGIPPRHRRQAVQFEKTRPAHEEIDLVAVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M57</accession>
    <name>Q67M57_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196377.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2253"/>
    <dbReference type="EnsemblBacteria" id="BAD41238">
        <property type="protein sequence ID" value="BAD41238"/>
        <property type="gene ID" value="STH2253"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2253"/>
    <dbReference type="eggNOG" id="COG2433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688726_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269901" evidence="1">
        <location>
            <begin position="37"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="222"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="300"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="46502" checksum="516F4D04E9D369E8" modified="2004-10-11" version="1">MGEWEPMKGDHTRRARWLAWLMLAALLVHGPVGAGAEEPLQPEVAEYPAENYEPGPGWPEGWTPPPPPEGMSEQMRAEVEQALRDNPDMYDRYDWWVWDPLPAWTQEYPWYWEEDWMVCGTTIHVYAYPKPDTHLIQQVFGRSYVVGRLHLKPCRYEPMQYIVAVRELSEEEKLAEGRGIEYEYWMDKSHPGLGRDYGYRHNGSPEEIFVFLNFGNASSHFDTPAYLDTTVNRVRVPIRFISEMMGAEVTWDQAGRRVTIHFPAVTREVVKAVPAPGYDYPDLIHPETHLPDPYRYLLQEQTVSVPERTIVLTIDHPVAVVDGHEVALDAPPVIRNDRTMVPVRFIAEQMGAKVYWVGAEPIYRLDDGTMSGRYQVHIFTPFFPLYEYPSWYLENRAVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M67</accession>
    <name>Q67M67_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2243"/>
    <dbReference type="EnsemblBacteria" id="BAD41228">
        <property type="protein sequence ID" value="BAD41228"/>
        <property type="gene ID" value="STH2243"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2243"/>
    <dbReference type="eggNOG" id="COG2433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688726_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269437" evidence="1">
        <location>
            <begin position="30"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="300"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="394" mass="46358" checksum="223EA83A9F2FBC69" modified="2004-10-11" version="1">MWGEQTRRVGWLTLLVLTVLLFQPVVGVAATDEPKPVVVDYPPENYEPGPGWPEGWSPPPPPEGMSEQMRLRVEQALRERPDLYERYTWWVWEPLPEWTKEYPWFWREDWMGCGTTLHVYAYPVPDTQLITRVFGRSYGWTRLYLEPCRYEPVQFIVEALEMSEEEKLAEGRRAEYLYWVDKSHPGLGRDDGRDRNGSPDHIYVYLNQGDASKRFDTPAYLDTTVNRVRVPIRFVSEMMGAEVTWDETGRRVTIHFPATTREVMKVVPAEGYDYPDLFDAEEYLPNGYRFYFEQRVVRIPERTITLTIDHPVAIVDGREVRLDAPPVIRNDRTMVPVRFVAEQMGAKVYWVGKEPIFRLDDGTMSGTYQVHIFTPTFPLYEYPSWYLENRAVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M95</accession>
    <name>Q67M95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2213"/>
    <dbReference type="EnsemblBacteria" id="BAD41198">
        <property type="protein sequence ID" value="BAD41198"/>
        <property type="gene ID" value="STH2213"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2213"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="812" mass="86623" checksum="DD9AFA5B3DF6F671" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA6</accession>
    <name>Q67MA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2202</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196327.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2202"/>
    <dbReference type="EnsemblBacteria" id="BAD41187">
        <property type="protein sequence ID" value="BAD41187"/>
        <property type="gene ID" value="STH2202"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2202"/>
    <dbReference type="eggNOG" id="COG2864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1194394_0_0_9"/>
    <dbReference type="OrthoDB" id="1612137at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="4">
        <location>
            <begin position="65"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="24852" checksum="9574831945C4AC62" modified="2004-10-11" version="1">MHGLTTGASRRHRLLPDRETLRDAGIELLSYVGLVGDQGVLGFPGLQWPTAWRREVERAVGFRGFPPEGKYLATQRLLSYPLWVLVSLVVVATGLLKAMRYAYPLPAAVVRWSTTLHDWAFIATIVLLAIHVAAVTVVRSNWPLLRSMFTGTVSLEHVRAVHGRWYLELLREMEANTAAGSAPTEAASAPAAAGSIPATAGPTPASAGPAPAGAGDVPRPDHTDRGGKEAPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD6</accession>
    <name>Y2172_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter STH2172</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH2172</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAE transporter (TC 2.A.81) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196297.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MD6"/>
    <dbReference type="SMR" id="Q67MD6"/>
    <dbReference type="STRING" id="292459.STH2172"/>
    <dbReference type="EnsemblBacteria" id="BAD41157">
        <property type="protein sequence ID" value="BAD41157"/>
        <property type="gene ID" value="STH2172"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2172"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035023_3_0_9"/>
    <dbReference type="OMA" id="AMWLIRL"/>
    <dbReference type="OrthoDB" id="1458740at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006512">
        <property type="entry name" value="YidE_YbjL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06826">
        <property type="entry name" value="Asp-Al_Ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01625">
        <property type="entry name" value="YidE_YbjL_dupl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000208780" description="Uncharacterized transporter STH2172">
        <location>
            <begin position="1"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal 1" evidence="2">
        <location>
            <begin position="186"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal 2" evidence="2">
        <location>
            <begin position="273"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="542" mass="58366" checksum="47FE6B8EA950DE77" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME5</accession>
    <name>Q67ME5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 14">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">STH2163</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="12 14">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 14"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196288.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2163"/>
    <dbReference type="EnsemblBacteria" id="BAD41148">
        <property type="protein sequence ID" value="BAD41148"/>
        <property type="gene ID" value="STH2163"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2163"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_9"/>
    <dbReference type="OMA" id="WASMLND"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12 14" id="KW-0274">FAD</keyword>
    <keyword evidence="5 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 12 14" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="16">
        <location>
            <begin position="5"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="15">
        <location>
            <begin position="352"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="184"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="13">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD41148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="471" mass="49592" checksum="85E6374FC6EAB422" modified="2004-10-11" version="1">MSHSFDVVVIGGGPGGYVAAIRGAQLGLRVALVEQDKVGGTCLHRGCIPTKAYLRSAEVLHTLRQSREYGVLTGGVDLDFAAVRSRKDKVVSNLHKGIQGLLKKNGVAVFQGRGALVPPSIFSPNGLVSVMVGDQQELLEFNKVIIATGSRPKTLGIPVDGAHVMTSDEALARPDLPASVIIIGAGVIGMEWASLYADFGVRVTVLEFLDRILPTEDEAVAAELHKLMTRRKVEIVTGAAVLPESVQAEGGQVRVQARVGEQVREFQAEALLIAVGREPVTRGFGLENRERVTYGPGGFIQVDGFGRTGDPAIYAIGDVAGGGLAHVASHQGIIAMEHIAGLNPEPFAPVRVPRCVYTRPEVASVGLTEAQAREQGREVRVGTFPWRGIGKALVHGSVDGFAKIVADAATGDVLGVHLIGPDATNLIAEAGLALTLNASAWELGQVIHPHPTLAEIFGEAALAVEGRAIHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG6</accession>
    <name>Q67MG6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41127">
        <property type="protein sequence ID" value="BAD41127"/>
        <property type="gene ID" value="STH2142"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2142"/>
    <dbReference type="HOGENOM" id="CLU_2792493_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7597" checksum="05F03A3BEC0D77CB" modified="2004-10-11" version="1">MEHGGGKTLAISREDVVSKLQALVESRGPWYIERLQVSDSEGNRYTPEVGEDGSVELVLRSGPSRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI3</accession>
    <name>Q67MI3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196250.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2125"/>
    <dbReference type="ESTHER" id="symth-q67mi3">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41110">
        <property type="protein sequence ID" value="BAD41110"/>
        <property type="gene ID" value="STH2125"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2125"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_9"/>
    <dbReference type="OMA" id="CWEALLW"/>
    <dbReference type="OrthoDB" id="1361074at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="2">
        <location>
            <begin position="78"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="33376" checksum="C9411353D5C68A23" modified="2004-10-11" version="1">MPLAREAEAFLKQLAEAGGPALNELSPADARKMTEQMAAFGGDAEPVAGVVDRTVPGPAGKIPVRIYTPAGEGPFPVLLYFHGGGWVLGSPDTVHATCALLANRAGAVVVSVDYRLAPEHKFPAAAEDCYAATVWVAENARTIGGDPRRIAVAGDSAGGNLAAVVSLMARDKGYPDLAYQVLIYPVTDHNFDTPSYRENGNDYFLTTAMMQWFWDHYIRSEADGRDWRASPLQAADVSGLPPAFVITAEYDPLRDEGEAYARKLIEAGSAVTVKRYLGQIHGFCTLLGAMPAGRQALEDAAAHLRLAFAARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN8</accession>
    <name>Q67MN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196195.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2070"/>
    <dbReference type="EnsemblBacteria" id="BAD41055">
        <property type="protein sequence ID" value="BAD41055"/>
        <property type="gene ID" value="STH2070"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2070"/>
    <dbReference type="eggNOG" id="COG3591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_857697_0_0_9"/>
    <dbReference type="OMA" id="WDGSRWG"/>
    <dbReference type="OrthoDB" id="376756at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="37689" checksum="512F97F0ED06CFAF" modified="2004-10-11" version="1">MQLPPWFMPGWPVPLAQRPPWGRPDWNNNDWNDRDDDDRDDGRDVEWNLRQEGGPLSRTGWSNWRRIGAGTTLDSPAPATYQNDLHLFVRGTDSGIYWNRMSRRTGWGEWWEVPGQGRTPSAPAATAYRGSLHLFVRGTDNRIYQNRLTGNRWSRWTEVPGGGLTTSSPAVTVHQGALYLAVRGTDNAVYVQRYDGQWQGWQRVPTGATSDAPAIVSYMGALWLFVRGTDNRIYYAVMRAPNQWSGWTEVPGGGLTPSGPGAVVYRGNLYVVVRGTNDGIYLNVRDPRGQWSGWTEVPGQGRTPSGPSGVRFADRLYLFVRGTDNGIYTTVRTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ0</accession>
    <name>Q67MQ0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2058"/>
    <dbReference type="EnsemblBacteria" id="BAD41043">
        <property type="protein sequence ID" value="BAD41043"/>
        <property type="gene ID" value="STH2058"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2058"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_1_9"/>
    <dbReference type="OMA" id="YADFKCG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="47"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="28570" checksum="8BFD67BC1A8A0B79" modified="2004-10-11" version="1">MVVVFQPYVPLQWTKLCQRSDVGMSKAKGGRSLNRKKDRSLWWVIGVAVGVTAALIVASNVTARRLGEIVLPSIILTDQERGADRHVLGSADAPVELVEFSDFRCPHCRESHEILGSQIEELVAEGTARYVRKHMLVIDPSDTSLNAAEAVECAADQGYYWAFLDMLFANQAAQGQKWTRDAMKTYARELGLDTKAFNECMDQQKYRDKVLADSAEGYSTPGITGTPSFLVNGELLRIRSYQDVISAVLAAAGQSPGQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ0</accession>
    <name>Q67MZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PTS system glucose-specific IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1968</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196095.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1968"/>
    <dbReference type="EnsemblBacteria" id="BAD40953">
        <property type="protein sequence ID" value="BAD40953"/>
        <property type="gene ID" value="STH1968"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1968"/>
    <dbReference type="eggNOG" id="COG2190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012312_5_3_9"/>
    <dbReference type="OMA" id="MIKKWKQ"/>
    <dbReference type="OrthoDB" id="1481289at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00371">
        <property type="entry name" value="PTS_EIIA_TYPE_1_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIA type-1" evidence="3">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="19109" checksum="BFD2329717A3AB0F" modified="2004-10-11" version="1">MRRSSFAIWQAQEGLAVVFGLFGKRSKPVTVAAPLAGEVRPISEAPDPVFAEKMLGDGFCVFPAAVDRTTVVSPVDGEVVNLFPTGHAVGLRTPDGLEVLVHVGIDTVKLQGRGFRALVAQGDRVSAGQPMLEVDLGSIQSDVPSLATPVLLTNLDDKRTWRLDRQGKVEAGEPVATVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N14</accession>
    <name>Q67N14_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1944"/>
    <dbReference type="EnsemblBacteria" id="BAD40929">
        <property type="protein sequence ID" value="BAD40929"/>
        <property type="gene ID" value="STH1944"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1944"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104527_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269906" evidence="1">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27664" checksum="0549AE4F49E67FD2" modified="2004-10-11" version="1">MRRHGMMGFVAGFVTAAVLFGGLAVAAGYERQITVHFRPLKYVFDGIERTPQGGEAGFIYQNRTYVPLRFIAESLGRPVEFVDGTIYVGAIPGKTPEVWNRLTEQGEGSFKVQFFADRALSLQGEEMPAATVVTLVAPGGDVEDKRGVTSQLWADYDLPAGVGRMSGTLYVPHQYFGLAGERRVGRLVVLNELNRPIYTSPDLTTKSDPVPFSVPLDGVKRVRIVVTLHPYEGVPLGDQLVMAQMGIAGLKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N53</accession>
    <name>Q67N53_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196032.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1905"/>
    <dbReference type="EnsemblBacteria" id="BAD40890">
        <property type="protein sequence ID" value="BAD40890"/>
        <property type="gene ID" value="STH1905"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1905"/>
    <dbReference type="HOGENOM" id="CLU_2119887_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269288" evidence="2">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12007" checksum="3535D9F4E02641FB" modified="2004-10-11" version="1">MNRRHRVHPRILPWLLIAALLVSGCSAGGPDPSAAAPTDRTENPGQTADPQMPEDPAGPDSAADDPPVAGAVPADLDGDGAPEWIRGLTDTWTADPVEIYDAQGEKLLTWETFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N56</accession>
    <name>Q67N56_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative serine esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196029.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1902"/>
    <dbReference type="ESTHER" id="symth-q67n56">
        <property type="family name" value="LYsophospholipase_carboxylesterase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40887">
        <property type="protein sequence ID" value="BAD40887"/>
        <property type="gene ID" value="STH1902"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1902"/>
    <dbReference type="eggNOG" id="COG0400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_5_3_9"/>
    <dbReference type="OMA" id="AMVWHSR"/>
    <dbReference type="OrthoDB" id="1195966at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_2" evidence="1">
        <location>
            <begin position="10"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="218" mass="23621" checksum="E0D8B1004832AA44" modified="2004-10-11" version="1">MLRYDQYVPSDARDGAPLFVLLHGRGSYKGDLMGLQPYLPASALVVTPEAPFPAAPWGYGPGWAWYRLQERGIPEPESFRESQRALAEFLAELPARLPVRPGPVILGGFSQGGVMSLGYALMHPGAVPMVINFSGFLPVHPDAAVTPVSVRGTRIFWGHGERDPAIPYELALEGQKRLRAAGADLTACTYPMGHAISPEELADMVTWVEQGLSRAMAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N57</accession>
    <name>Q67N57_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1901"/>
    <dbReference type="EnsemblBacteria" id="BAD40886">
        <property type="protein sequence ID" value="BAD40886"/>
        <property type="gene ID" value="STH1901"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1901"/>
    <dbReference type="eggNOG" id="COG4684">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088550_1_0_9"/>
    <dbReference type="OMA" id="SIMYGPR"/>
    <dbReference type="OrthoDB" id="1466380at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="21519" checksum="41D77AF301554F1B" modified="2004-10-11" version="1">MEECLYCYQTHMGVTTSKGEGCTMNMSVRKMAVAGLLGAVSAALGLVPGLGFIPFPTGQAVTIMQIPVVLAAVAEGPAVGLFVGLIFGLTSFLRATNPAFADPLVSILPRLFIGVFAAYAFAALRRRNLTLALAAAGVVGTLTNTGLVLGMILLRGYWPPEVVWTVAVTHAPLELLVGTFAVVTVGLALHRAGYIRVAYRPSGQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N58</accession>
    <name>Q67N58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1900"/>
    <dbReference type="EnsemblBacteria" id="BAD40885">
        <property type="protein sequence ID" value="BAD40885"/>
        <property type="gene ID" value="STH1900"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1900"/>
    <dbReference type="eggNOG" id="COG3191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044458_1_0_9"/>
    <dbReference type="OMA" id="PHFWAAP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="PTHR36512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="32899" checksum="0BC653265F0D382D" modified="2004-10-11" version="1">MLPMTDPVWLSGITDVPGIRVGHAHDLQALTGVTAVLAPDGAVVGVAVEGSAPGTRETDLCRPCNLVQQAHAVLLCGGSAFGLAAAHGAMRWLEERGIGLPTGYATVPIVPAAVLYDLGVGSAQVRPDEAMGYAACEAATDGPVEQGCIGAGAGCTVGKALGMDRAVKSGIGTACVKVGAVAVGAIVAVNAFGDVRDPATGRILAGPRGEDGTYLDTTALLLQGAAGRPLAENTTIGVVAVGGSLSKEGANKVARMAHDGLARTISPIHTMFDGDTLFCLATGGPEVDVTTAGVAAALAVERAVLNAVRHAASMGGLPAARAWSCPVKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB5</accession>
    <name>Q67NB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1843"/>
    <dbReference type="EnsemblBacteria" id="BAD40828">
        <property type="protein sequence ID" value="BAD40828"/>
        <property type="gene ID" value="STH1843"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1843"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096497_0_0_9"/>
    <dbReference type="OMA" id="KPFHAMA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="20871" checksum="962BD6047E908183" modified="2004-10-11" version="1">MAKEPPPIPGWRRAVRVGLLSFLLAVVVNWSSNLALHRVHAIIAFVIVLLLIAVNIVFDILGTSVTAAELPPLNSMAAKKVPGAKQAIWLVRNADAVANFCNDVVGDVAGAVTGAAGATVAMKLARMMQGASWVEEALGLLIIGVISGLTVGGKAAGKSFAIENATTAVMAAGRIIYWAENITGRSFTGGRSGSNGRRRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC0</accession>
    <name>Q67NC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195966.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1838"/>
    <dbReference type="EnsemblBacteria" id="BAD40823">
        <property type="protein sequence ID" value="BAD40823"/>
        <property type="gene ID" value="STH1838"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1838"/>
    <dbReference type="HOGENOM" id="CLU_1757900_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="14518" checksum="A235D3CEBFDAA4D9" modified="2004-10-11" version="1">MQRTYPAPAPLPWPPPVLPAPGPAALGPAGVQGGRGTGVPGVAFVPVTAVAPPAQPGGPHPLPALPPCPLPWGFPNPFAIRAAVVPAPPAQSGSQSWPARPGMVTVTGPAGYLPGIGYFDPLQVAAAAAPLLEAAAQTPPGPYAPPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI9</accession>
    <name>Q67NI9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195897.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1769"/>
    <dbReference type="EnsemblBacteria" id="BAD40754">
        <property type="protein sequence ID" value="BAD40754"/>
        <property type="gene ID" value="STH1769"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1769"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026152_3_1_9"/>
    <dbReference type="OMA" id="RINWNAT"/>
    <dbReference type="OrthoDB" id="848990at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="204"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="408" mass="44423" checksum="9898EDABD319CE40" modified="2004-10-11" version="1">MRLGSGSGLQWLGAAALLLAALWLRSGLLLLAAGLVALVAGVVALWRRFGLSRLTYERHFQQNRCFPGEPVTLTVTVTNRKVLPLTYLVVEESVPVALEVESRRLHFQSRARGRLRMRFSLGWYQRVERRLTVRAARRGAYRLGPAVVAAGDPFGWAEQRMEVAPSDLLIVYPRVVPLEEVGLPAQRPFGDLASRDRLFADPLRFAGVREYRPGDPLNQVHWKATAATGELQVRLLDPSASLGLAVFLNTWSYERFWEGDDADALEAGVTLAASIVGWAHEHALPVGLYANGLVYGWGFSLHLPPARGDGVLVQALEGLARLQTGSPQPLWELMAAEVPALPYGTSLVVITRQVGVDLAGAVLRAQRSGRPVTLVVTGPEAEPVPELPGVRVYRVGGEEGLHGAVLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT1</accession>
    <name>Q67NT1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195805.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1677"/>
    <dbReference type="EnsemblBacteria" id="BAD40662">
        <property type="protein sequence ID" value="BAD40662"/>
        <property type="gene ID" value="STH1677"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1677"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027872_0_0_9"/>
    <dbReference type="OMA" id="RWASGHI"/>
    <dbReference type="OrthoDB" id="991634at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="369"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="660" mass="73658" checksum="FD63154362F958E2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU5</accession>
    <name>Q67NU5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195792.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1663"/>
    <dbReference type="EnsemblBacteria" id="BAD40648">
        <property type="protein sequence ID" value="BAD40648"/>
        <property type="gene ID" value="STH1663"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1663"/>
    <dbReference type="HOGENOM" id="CLU_1795498_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="16681" checksum="FA19750F4B538BAD" modified="2004-10-11" version="1">MQTPLRPLGISPADLEAELAARRASHRAQVDLLKARLAEAGARNAQLRQRRAGLQAERERLEQEIQRVLEEFDRTGTEFEVRRREVAERHRRELADREAELEAVRKERDNWIALERQIAEGILAAVQPFLQLQAYLREQEGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX2</accession>
    <name>Q67NX2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative GTP pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1636"/>
    <dbReference type="EnsemblBacteria" id="BAD40621">
        <property type="protein sequence ID" value="BAD40621"/>
        <property type="gene ID" value="STH1636"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1636"/>
    <dbReference type="eggNOG" id="COG2357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058756_0_0_9"/>
    <dbReference type="OMA" id="GCANEVM"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RelA_SpoT" evidence="2">
        <location>
            <begin position="88"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="46223" checksum="CF6223513FD7738C" modified="2004-10-11" version="1">MSFPLPSRSIRDRSLFARRRFRRRQGRNGPVHIGCANEVMQLMNGKQREWVETFRSQQALYQAFGQCVQQLLSHLLDEANIPINGITYRVKSVDALAEKLTRPGKDYESLIEVTDLVGVRVVTNFADDVDRVVRVIDRHFEVDLDHSEDKRPHDPHWFGYSSVHRVCLLPPAVLARPEYARFAGLRCEIQVRSILQHAWAEIEHDLGYKVAGGIPSSLRRRFSMLAGLLELADDQFMRLRDELSLYSTRVVAQMHDHPESVALDPISLETFIRRSDLVQALDEEISEMRGRPLMGPTKAVLEARVEELEHLGITNLKDLADALEQSQERVRSLARQIYRDDRRRGLPMSRGSCLSLLVQVLIGRRESLDEIRALLDRFNIGAPAARDERALLLRSMAQGLPAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX8</accession>
    <name>Q67NX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195759.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1630"/>
    <dbReference type="EnsemblBacteria" id="BAD40615">
        <property type="protein sequence ID" value="BAD40615"/>
        <property type="gene ID" value="STH1630"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1630"/>
    <dbReference type="HOGENOM" id="CLU_521682_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="522" mass="52909" checksum="DF994F9E28C3D052" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P53</accession>
    <name>Q67P53_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195684.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1555"/>
    <dbReference type="EnsemblBacteria" id="BAD40540">
        <property type="protein sequence ID" value="BAD40540"/>
        <property type="gene ID" value="STH1555"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1555"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140387_0_0_9"/>
    <dbReference type="OMA" id="RGGCWFR"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="13398" checksum="DF1723DED3BF736C" modified="2004-10-11" version="1">MLVGLHHVLIAAPPGCEEEARAFYGGLLGLPEIPKPAGLRERGGVWFRCGGQEVHVGVEEDFRPARKAHPAFLVQDLDGLRQRLVNRGIPVTDDVPLPGCRRFHTRDPFGNRLEFLQPCPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P61</accession>
    <name>Q67P61_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="12">ASA dehydrogenase</shortName>
            <shortName evidence="12">ASADH</shortName>
            <ecNumber evidence="7 12">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">asd</name>
        <name evidence="14" type="ordered locus">STH1547</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="7 12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1547"/>
    <dbReference type="EnsemblBacteria" id="BAD40532">
        <property type="protein sequence ID" value="BAD40532"/>
        <property type="gene ID" value="STH1547"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1547"/>
    <dbReference type="eggNOG" id="COG0136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049966_0_1_9"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005986">
        <property type="entry name" value="Asp_semialdehyde_DH_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01296">
        <property type="entry name" value="asd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="13">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="38"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="337" mass="36840" checksum="CBBD9EEAD44FE78F" modified="2004-10-11" version="1">MKNVAVVGATGAVGQELLSLLEARNFPVGRLLPLASSRSAGSTVSFRGERLMVQEATPEAFAGMDLVFFAATGLLSRELAPAAARAGAVVIDKSSTWRMDPEVPLVVPEVNPEDLRRHKGIIASPNCTTIGLVMALKPLHDLARITRVIVTTMQAVSGTGKEAIEELEQQVRAWTEGHRGELPHTVYKRQIAFNVLPYAETFTENLYTTEEMKLSAETRKIMGLPDLPVTMTCTRVPVFVGHSESVLVEFERKVTPAEAREALSRFPGVRVVDDPLQFVFPTAIDAAGTDDTLVGRIREDLTNEKGLWLWIVSDNLRKGAATNAVQIAEKLLEMQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P68</accession>
    <name>Q67P68_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cheY</name>
        <name evidence="5" type="ordered locus">STH1540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195670.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1540"/>
    <dbReference type="EnsemblBacteria" id="BAD40525">
        <property type="protein sequence ID" value="BAD40525"/>
        <property type="gene ID" value="STH1540"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1540"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_9"/>
    <dbReference type="OMA" id="APILMIS"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="119" mass="13218" checksum="BD84B4EA21CD32CC" modified="2004-10-11" version="1">MPKILLVDDAAFMRMRCAKLLTEHGFEVAEAENGQEAIAMYQSYRPDLVLMDITMPVMDGITATREIKNLDPDAKVVMVSALGQQTMVIEAIKAGAKDFVVKPFEPEKILSTVRKFVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC4</accession>
    <name>Q67PC4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTP-sensing transcriptional pleiotropic repressor CodY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">codY</name>
        <name evidence="10" type="ordered locus">STH1484</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-binding protein that represses the expression of many genes that are induced as cells make the transition from rapid exponential growth to stationary phase. It is a GTP-binding protein that senses the intracellular GTP concentration as an indicator of nutritional limitations. At low GTP concentration it no longer binds GTP and stop to act as a transcriptional repressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CodY family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195614.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1484"/>
    <dbReference type="EnsemblBacteria" id="BAD40469">
        <property type="protein sequence ID" value="BAD40469"/>
        <property type="gene ID" value="STH1484"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1484"/>
    <dbReference type="eggNOG" id="COG4465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089581_0_0_9"/>
    <dbReference type="OMA" id="TETHANF"/>
    <dbReference type="OrthoDB" id="977877at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00621">
        <property type="entry name" value="HTH_type_CodY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014154">
        <property type="entry name" value="CodY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010312">
        <property type="entry name" value="GTP_sensing_CodY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013198">
        <property type="entry name" value="GTP_sensing_HTH_CodY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40062">
        <property type="entry name" value="PTHR40062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06018">
        <property type="entry name" value="CodY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08222">
        <property type="entry name" value="HTH_CodY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011572">
        <property type="entry name" value="GTP_sensing_CodY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02787">
        <property type="entry name" value="codY_Gpos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CodY" evidence="8">
        <location>
            <begin position="3"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_CodY" evidence="9">
        <location>
            <begin position="192"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="7">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="27228" checksum="9759A086CB8A2A92" modified="2004-10-11" version="1">MKTLLEKTRRINRLLQKSAGTQVNFNELAGVLADLIGADVYVASRKGNIVGLGLTAGDADAYQNITFTEEYNSSLMKVDDTTANTEQSELTVLQEGREVQMRGGRVTIVPVNGGGERLGTLVLVKSADLTDEDLILGELAATVAGVEILRAHAGELEEEARKKAAVQIALATLSYSELEAVQHIFDELDGNEGLLVASKIADRVGITRSVIVNALRKFESAGVIESRSLGMKGTHIKVLNDRLIEELRKLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF8</accession>
    <name>Q67PF8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="3">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fabD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195580.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1450"/>
    <dbReference type="EnsemblBacteria" id="BAD40435">
        <property type="protein sequence ID" value="BAD40435"/>
        <property type="gene ID" value="STH1450"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1450"/>
    <dbReference type="eggNOG" id="COG0331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_0_1_9"/>
    <dbReference type="OMA" id="AANYNCP"/>
    <dbReference type="OrthoDB" id="1123553at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="5">
        <location>
            <begin position="7"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="314" mass="33877" checksum="F91C278F6E6EEBC3" modified="2004-10-11" version="1">MAKIAFLFPGQGAQYVGMGLELAREFPEVARRFEQMNELVGFDLRKLCWEGPEEELKKTANQQPAILALSVACLELLLREGIRPDVTAGLSLGEYSALVAAGVLTFEEAVPLVRRRGTYMQEAVPLGTGGMAALLGLTAEQVDELCARAARETGRHVEGANYNCPGQVVVAGHTEALERCMALAVEAGGRAVRLSVTAPFHSRLLTPAGERLAEALAAVPLREAQVPVVSNVTADYVTQPGEIRRLLIEQVSRPVLWEASIRRMIADGVDTFVEVGPSRTLTGFLRKIDKGVRALHVEDPGSLARVLDSLGRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU6</accession>
    <name>Q67PU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1312</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195443.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1312"/>
    <dbReference type="EnsemblBacteria" id="BAD40297">
        <property type="protein sequence ID" value="BAD40297"/>
        <property type="gene ID" value="STH1312"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1312"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_9"/>
    <dbReference type="OMA" id="IMSVWGI"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="102"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="37848" checksum="D16B0F19599D5C71" modified="2004-10-11" version="1">MQRGIPVSNPYLYTLVRWVAVGVGMLLLMLLTYGLLRAFRARRSTAAGYALILPWLLGLIIWQLFPILYSLYLSFTAYDVLTPPRWVGLKNYVDMFTRDTRFWPSVRLTVLYSVISVPLGVLGALGTASLLAREVRGIGFWRTLYYVPAVLPAAATALLWRWMFASRGLINSLLSPVYRLLNMEPLSWFSDERLVLPSFIIMSFWGVFGANTVILLAALKNVPKELKEAALVDGAGPWTVFSRITVPMISPALLYVTIMGVIGAMQNFTAPLFIATPSSAGTFLNVYVYQQGFTQFKMGYASAVAWFLMVLILILTAIVWKWSDAYVYYEGELRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV5</accession>
    <name>Q67PV5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195434.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1303"/>
    <dbReference type="EnsemblBacteria" id="BAD40288">
        <property type="protein sequence ID" value="BAD40288"/>
        <property type="gene ID" value="STH1303"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1303"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023846_0_0_9"/>
    <dbReference type="OMA" id="SHQRIYG"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="216"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="237"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="693" mass="78513" checksum="A4FBB06873E9D165" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX1</accession>
    <name>Q67PX1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1287"/>
    <dbReference type="EnsemblBacteria" id="BAD40272">
        <property type="protein sequence ID" value="BAD40272"/>
        <property type="gene ID" value="STH1287"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1287"/>
    <dbReference type="HOGENOM" id="CLU_972645_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="331" mass="36263" checksum="2EF2D7BD61A84880" modified="2004-10-11" version="1">MRLIIILMPDGKSQHHRPVHPGGVCMQDDTAHIPGGTWSDSPLRPSLERFAALHTRADVRWGHPEESDDGYRYEALYDPGFIGRFLDQIAAEFAGPPAFSGTRLFRVAHFLPLRLAGYLFAAEGRVIRLRENLVLRNAAHRQGSHPHGHHPHGGHPHGGRPPGLRVLEPRAVVLPDDPAAGAPGIETAPDRAALADALFAEVTALAEPLMRRLHDLGVLPPGTGWGILLDFLAAGFLAAGRAGTGLDAAWAAWEEAIAGRTFPARRLPRRLQFTADGRTDEIMVHAHCCLRCALPHMKDREDRHCIECYLESDENRIARLLGRRAAGAHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q43</accession>
    <name>Q67Q43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative cell division protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1215</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1215"/>
    <dbReference type="EnsemblBacteria" id="BAD40200">
        <property type="protein sequence ID" value="BAD40200"/>
        <property type="gene ID" value="STH1215"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1215"/>
    <dbReference type="eggNOG" id="COG1589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1015369_0_0_9"/>
    <dbReference type="OMA" id="IKMYTRS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005548">
        <property type="entry name" value="Cell_div_FtsQ/DivIB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013685">
        <property type="entry name" value="POTRA_FtsQ_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03799">
        <property type="entry name" value="FtsQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08478">
        <property type="entry name" value="POTRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="58"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="30122" checksum="C1852211D54E3824" modified="2004-10-11" version="1">MSLRICGAARRGGDGVTTSAVPMKGKGSARARSRWGFVLALAVLLGICLFAAYRSALFRLERVQIGGNERLSQAEVMAIAGVMPGDLKWEVTAERVRQRLLADPWVESAGVTWRGNALVITVTEREPLALLQYHGRFYLVLDAEGRVLGQRLLEEGERLPVVSGVTVERALRGDVLDDLGLKDALTLLWWTGEPLLEQLSEVHVRADRYLRLYLTGGTTVEVGVLPDDAAAREDHIQTQLRGLLDQLDRVPASARGRCQIDLRVYGKVLAQGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q69</accession>
    <name>Q67Q69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA carboxylase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1189"/>
    <dbReference type="EnsemblBacteria" id="BAD40174">
        <property type="protein sequence ID" value="BAD40174"/>
        <property type="gene ID" value="STH1189"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1189"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_2_9"/>
    <dbReference type="OMA" id="YAYAECT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="257"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="513" mass="55437" checksum="CA5E0135C3BCDBBB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA9</accession>
    <name>Q67QA9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1149</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1149"/>
    <dbReference type="EnsemblBacteria" id="BAD40134">
        <property type="protein sequence ID" value="BAD40134"/>
        <property type="gene ID" value="STH1149"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1149"/>
    <dbReference type="eggNOG" id="COG0171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_865804_0_0_9"/>
    <dbReference type="OMA" id="TPPRIRM"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="7">
        <location>
            <begin position="31"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="34587" checksum="BE29189F955582B3" modified="2004-10-11" version="1">MAEATAGAQGGWSMTQQHRTRSLAERAAGEREYMARSGIRTLINSLSGGVDSSTVAAISKQAAPDGALGLILPCSTPEEMKGERLQDVRDAERVAAHLGIPAVTIDLSDLWQQAAALFSAAARELAEKAGVPLEEERLQWAIHNMKPTLRIMTAGFFADTFRGLMMGTGNGVEYFLGYFSLRGDGISDRQPIRDCTKEEVRAMAASAGLPEDLVHRVPTAGLWPGQTDEGELGFSYADADRFLVWILNRHVAEPCLTTTLTVREESVEAILADPGLPVAAEVARRIIDQNRRTAFKRRAGDLEAMLAARGLAPGATGGRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC7</accession>
    <name>Q67QC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IstB helper protein N-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1131"/>
    <dbReference type="EnsemblBacteria" id="BAD40116">
        <property type="protein sequence ID" value="BAD40116"/>
        <property type="gene ID" value="STH1131"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1131"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_5_0_9"/>
    <dbReference type="OMA" id="NGENVIF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="10"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="15084" checksum="5877C0727B780C9B" modified="2004-10-11" version="1">MLELEQVRQQLESLGLSQAATVLDRCLEVGTRENLPALTVVRHILEEELAARHERYLKARTRLAHLPFHKTLADFDFTAQPSLDERQVRDLATLRFLSNGENVIFLGPPGVGKTHLAVALALKAISQAPTSSPHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD3</accession>
    <name>Q67QD3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1125"/>
    <dbReference type="EnsemblBacteria" id="BAD40110">
        <property type="protein sequence ID" value="BAD40110"/>
        <property type="gene ID" value="STH1125"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1125"/>
    <dbReference type="HOGENOM" id="CLU_2686480_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8882" checksum="5B1E24F7079F1A53" modified="2004-10-11" version="1">MNLAMHCHPDTAYRIAEMRSLEYNPHLAVHRHAIEEMLRARRAEAALRRRFFLRFWLRSILFRLAPGLARRLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE7</accession>
    <name>Q67QE7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195243.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1111"/>
    <dbReference type="EnsemblBacteria" id="BAD40096">
        <property type="protein sequence ID" value="BAD40096"/>
        <property type="gene ID" value="STH1111"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1111"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OMA" id="QRMQIIF"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="7"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="55"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="36180" checksum="B39CE31B3E38C04D" modified="2004-10-11" version="1">MSAAPLLEVRDLYKHFVLPRSLLSRALTGQGHRVVQAVNGVSFALQQGETLGLVGESGSGKSTLGRTILRLYEPTRGEVRFDGQDVFRLDAAGLRRFRQQAQIVFQNPYASLNPRKTVRQILAVPLALRGLSRRERQEEAAALMERVGLSPQHLDRYPHQFSGGQRQRIGIARALAMHPRLIVADEPVSALDVSVQAQIINLLEDLQQELGLTYLFISHDLSVVRHLSTRVAVMYLGQIVELSPTEPLFEEPLHPYTRALLSAAPGQGRRERIILQGAPPSPLDPPPGCPFHPRCPAVVGDVCRTDRPAPVLLGEGRWVACHLYGPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG4</accession>
    <name>Q67QG4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium uptake system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195226.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1094"/>
    <dbReference type="EnsemblBacteria" id="BAD40079">
        <property type="protein sequence ID" value="BAD40079"/>
        <property type="gene ID" value="STH1094"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1094"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_3_2_9"/>
    <dbReference type="OMA" id="AIGANIQ"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="2">
        <location>
            <begin position="135"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="23236" checksum="CAF426051E6DAC88" modified="2004-10-11" version="1">MAKRYAVIGLGRFGASLAQELTGAGQHVLAVDVDADRVDELAAVLPRVVRADGTDPAVLRALRIHEFDTVVVAIGDNVESSVLTVLNCRDLGVPYLVAQAQDEAHGRILKRLGVDRVVYPQRDMGIRVASNIATGGIIDYVRLSDEYGLAELAPPRSVLGKSLRDLDLPHRFGLNVMAIKRGQRVIVSPRAEERIAEGDIMVVIGSAAGITRLQGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG6</accession>
    <name>RL35_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmI</name>
        <name type="ordered locus">STH1092</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195224.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QG6"/>
    <dbReference type="SMR" id="Q67QG6"/>
    <dbReference type="STRING" id="292459.STH1092"/>
    <dbReference type="EnsemblBacteria" id="BAD40077">
        <property type="protein sequence ID" value="BAD40077"/>
        <property type="gene ID" value="STH1092"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1092"/>
    <dbReference type="eggNOG" id="COG0291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169643_4_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_L35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000258769" description="50S ribosomal protein L35">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="64" mass="7523" checksum="66A8D3DDE281871D" modified="2004-10-11" version="1">MPKMKSHRGAAKRFKLTGSGLVKHYPSNKHHKNTHKKENRIRALKRNLVLSKSFQKNIREILPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH5</accession>
    <name>Q67QH5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1083</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1083"/>
    <dbReference type="EnsemblBacteria" id="BAD40068">
        <property type="protein sequence ID" value="BAD40068"/>
        <property type="gene ID" value="STH1083"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1083"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="EQGQSEV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="221"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="321"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="518"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="577"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="635" mass="67333" checksum="B2FD11119E64A71B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI2</accession>
    <name>Q67QI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1076"/>
    <dbReference type="MEROPS" id="M23.A06"/>
    <dbReference type="EnsemblBacteria" id="BAD40061">
        <property type="protein sequence ID" value="BAD40061"/>
        <property type="gene ID" value="STH1076"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1076"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713047_0_0_9"/>
    <dbReference type="OMA" id="YRITIEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="94"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="1">
        <location>
            <begin position="240"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="360" mass="39433" checksum="EEE0B58F939CE443" modified="2004-10-11" version="1">MAERRLNIMSIPIDIRRGWWVRLAVRVDGPETRSRHTRADGGGSTMQRSWKRRLLAALALAALLGTVPVPAAAAGYKVISNGVVVSDVQAEMVDGQLMTAVGPFAESLGYKASYDSKSKMITVYKSGYWLTMWIGTHLAFQNDREVWSPVKPYIKNGQAMVPAWWLAVRLGADVSYANGTLTVSNGYKAPQPATGVNPNPRPDSPFADPTYVFPFPQGATYSAYADTMGASRYWDGQTFGHEGTDIPAPHGTPIVAVASGTIVRYGWNTLGGYRITIELDDHPGYYFYYAHLSGYAPGLKLGSHVKKGQLLGYVGSTGEGPEGTSGKFIDHLHFGIYDSNWKPINSYPLLKYWEGNKVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX3</accession>
    <name>Q67QX3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-lactamase</fullName>
            <ecNumber evidence="1">3.5.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-lactam + H2O = a substituted beta-amino acid</text>
            <dbReference type="Rhea" id="RHEA:20401"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35627"/>
            <dbReference type="ChEBI" id="CHEBI:140347"/>
            <dbReference type="EC" id="3.5.2.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-D beta-lactamase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195067.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH935"/>
    <dbReference type="EnsemblBacteria" id="BAD39920">
        <property type="protein sequence ID" value="BAD39920"/>
        <property type="gene ID" value="STH935"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH935"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676023_0_0_9"/>
    <dbReference type="OrthoDB" id="991474at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002137">
        <property type="entry name" value="Beta-lactam_class-D_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00337">
        <property type="entry name" value="BETA_LACTAMASE_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="3">
        <location>
            <begin position="181"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="407" mass="43205" checksum="6F165519AEB224CD" modified="2004-10-11" version="1">MQRAVTAGILWGVVGLAAAVGAAWLVARPAEPAILASPAGSGGAPDGRRAQQGAGREVYAQPALANRAEQVGELFPAGSAPLVGQAEPVGQAEPVGQAEPVGQAEPVDPVEPVGQAAVDRAGLPPAAHPLADYQPMHVEPVEPIIPAPPEDVPELWRPLHETVYAVAVEYPGRISVVAVDLTTGSRYEFRARDPYLPASTFKLPVALCVLEAIDAGEMAWNTLVTYTEEDYEPVGAGGFAQAAFGSRWTVRNLVDRSLISSNNVAVKMLARTLTWDGLLACTTAMGGPVTRTEEGSTPVSAADEAAWWLRLWELSRERPELAEQLLRPLRQATYRGRIQAGTPRPELVTHKFGTYAGYDHDGGIVWAERPYALVVLTYGGGEYLADRAIERIAAAAWASVMGDAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R26</accession>
    <name>Q67R26_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell wall degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195014.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH882"/>
    <dbReference type="EnsemblBacteria" id="BAD39867">
        <property type="protein sequence ID" value="BAD39867"/>
        <property type="gene ID" value="STH882"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH882"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010591_3_0_9"/>
    <dbReference type="OMA" id="IYWEVAP"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="9"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="72"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="118" mass="13124" checksum="F60B56AF70604036" modified="2004-10-11" version="1">MPQCPPGSFSYTVRPGDNFWTLAWRFGTTPQAIARANPGVNSWSLRIGQTLCIPGWSPWVTPPQGRCPQGWEPYRIQPGDTLGSIAWERQTTVANLLRVNPVNPNNLRIGQIICVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R61</accession>
    <name>Q67R61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1346</name>
        <name evidence="5" type="ordered locus">STH1550</name>
        <name evidence="6" type="ordered locus">STH1671</name>
        <name evidence="2" type="ordered locus">STH20</name>
        <name evidence="7" type="ordered locus">STH2014</name>
        <name evidence="8" type="ordered locus">STH2057</name>
        <name evidence="9" type="ordered locus">STH2559</name>
        <name evidence="10" type="ordered locus">STH2924</name>
        <name evidence="11" type="ordered locus">STH2953</name>
        <name evidence="12" type="ordered locus">STH3110</name>
        <name evidence="13" type="ordered locus">STH3121</name>
        <name evidence="14" type="ordered locus">STH3313</name>
        <name evidence="3" type="ordered locus">STH847</name>
    </gene>
    <organism evidence="3 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">IAM14863</strain>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194155.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39005">
        <property type="protein sequence ID" value="BAD39005"/>
        <property type="gene ID" value="STH20"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39832">
        <property type="protein sequence ID" value="BAD39832"/>
        <property type="gene ID" value="STH847"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40331">
        <property type="protein sequence ID" value="BAD40331"/>
        <property type="gene ID" value="STH1346"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40535">
        <property type="protein sequence ID" value="BAD40535"/>
        <property type="gene ID" value="STH1550"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40656">
        <property type="protein sequence ID" value="BAD40656"/>
        <property type="gene ID" value="STH1671"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40999">
        <property type="protein sequence ID" value="BAD40999"/>
        <property type="gene ID" value="STH2014"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41042">
        <property type="protein sequence ID" value="BAD41042"/>
        <property type="gene ID" value="STH2057"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41544">
        <property type="protein sequence ID" value="BAD41544"/>
        <property type="gene ID" value="STH2559"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41907">
        <property type="protein sequence ID" value="BAD41907"/>
        <property type="gene ID" value="STH2924"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41936">
        <property type="protein sequence ID" value="BAD41936"/>
        <property type="gene ID" value="STH2953"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42092">
        <property type="protein sequence ID" value="BAD42092"/>
        <property type="gene ID" value="STH3110"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42103">
        <property type="protein sequence ID" value="BAD42103"/>
        <property type="gene ID" value="STH3121"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42294">
        <property type="protein sequence ID" value="BAD42294"/>
        <property type="gene ID" value="STH3313"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1346"/>
    <dbReference type="KEGG" id="sth:STH1550"/>
    <dbReference type="KEGG" id="sth:STH1671"/>
    <dbReference type="KEGG" id="sth:STH20"/>
    <dbReference type="KEGG" id="sth:STH2014"/>
    <dbReference type="KEGG" id="sth:STH2057"/>
    <dbReference type="KEGG" id="sth:STH2559"/>
    <dbReference type="KEGG" id="sth:STH2924"/>
    <dbReference type="KEGG" id="sth:STH2953"/>
    <dbReference type="KEGG" id="sth:STH3110"/>
    <dbReference type="KEGG" id="sth:STH3121"/>
    <dbReference type="KEGG" id="sth:STH3313"/>
    <dbReference type="KEGG" id="sth:STH847"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="WVKQRYF"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD42103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD42294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49870" checksum="77DEBAD11D52D25C" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREGLLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRRLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWKQWKRVRTRFRELRALGLPEWVVHQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R86</accession>
    <name>Q67R86_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH822</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194955.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH822"/>
    <dbReference type="EnsemblBacteria" id="BAD39807">
        <property type="protein sequence ID" value="BAD39807"/>
        <property type="gene ID" value="STH822"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH822"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_3_0_9"/>
    <dbReference type="OMA" id="NVVNFTH"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="31063" checksum="FB882E15EF430C48" modified="2004-10-11" version="1">MGQFAEQVVNGLQLGFVYALIAVGYTMVYGIIKLINFAHGDVIMVGAFVTFFGLVRGIPWPAAILTGMATCALLGVVIERLAYRPLRVKGAPRIAALITAIGVSLFLENFASLRIAFGPDHRQFPAMVTRLQWNVFGVTISSVQVLIAGVTIVLVLILQLVVYRTKIGKAMRAVALDLDAARLMGINVDRVIAFTFAIGSALAAAAGALWAAAYPQIWPYMGIPPGLKAFTAAVLGGIGSIPGALLGAVLMGQAEVLTAAYITTDFRDAVAFALLILVLLVRPAGLLGKAGPEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA9</accession>
    <name>Q67RA9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH799</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH799"/>
    <dbReference type="EnsemblBacteria" id="BAD39784">
        <property type="protein sequence ID" value="BAD39784"/>
        <property type="gene ID" value="STH799"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH799"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="VTVFMVE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030660">
        <property type="entry name" value="ABC_branched_ATPase_LivF/BraG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039137">
        <property type="entry name" value="ABC_branched_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="237" mass="25439" checksum="857E6B503CA23E15" modified="2004-10-11" version="1">MPLMLELQGVSAAYGKIRALSDLTLTVPDGQIVTLLGANGAGKSSTLKVVTGLLRPSAGRVLFDGHDLARMAPETIVPLGISMVPEGRQVFAHLTVAENLRMGAYARKDAAGVRRDLEWVLALFPRLKERYGQEAGTLSGGEQQMLAIARALMARPRLLLLDEPSLGLAPLVVREIFATIRQVNAEGMSVLLVEQNANLALQVAHHAYILETGRLVLSGPAGELRLHPEVKRAYLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB3</accession>
    <name>Q67RB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PTS system fructose-specific IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194928.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH795"/>
    <dbReference type="EnsemblBacteria" id="BAD39780">
        <property type="protein sequence ID" value="BAD39780"/>
        <property type="gene ID" value="STH795"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH795"/>
    <dbReference type="eggNOG" id="COG1762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072531_5_0_9"/>
    <dbReference type="OMA" id="GIDYNSM"/>
    <dbReference type="OrthoDB" id="1810962at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00211">
        <property type="entry name" value="PTS_IIA_fru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004715">
        <property type="entry name" value="PTS_IIA_fruc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00848">
        <property type="entry name" value="fruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00372">
        <property type="entry name" value="PTS_EIIA_TYPE_2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PTS EIIA type-2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="15482" checksum="74B0114925F867A2" modified="2004-10-11" version="1">MSIRELITPETITLDLKAGRPEEAMAELGSLLVASGAVTDLETYLEAVKAREAQGTTAVGFGVAIPHGKSSAVSRAAVAFGRSRGALQWESLDGEPVKMVFLIAAPDGAHDLHLKALSQLARLLMHEEVREKLLTAASPADVIAALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC0</accession>
    <name>Q67RC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194921.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH788"/>
    <dbReference type="EnsemblBacteria" id="BAD39773">
        <property type="protein sequence ID" value="BAD39773"/>
        <property type="gene ID" value="STH788"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH788"/>
    <dbReference type="eggNOG" id="COG2766">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_028588_2_0_9"/>
    <dbReference type="OrthoDB" id="49410at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013153">
        <property type="entry name" value="Prk_AAA_dom"/>
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    <dbReference type="InterPro" id="IPR010650">
        <property type="entry name" value="PrkA_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016230">
        <property type="entry name" value="Ser_kinase_PrkA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08298">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06798">
        <property type="entry name" value="PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000549">
        <property type="entry name" value="Ser_prot_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00763">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA_PrkA" evidence="1">
        <location>
            <begin position="21"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="631" mass="72677" checksum="74D1DACB347CAF21" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG6</accession>
    <name>Q67RG6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH742"/>
    <dbReference type="EnsemblBacteria" id="BAD39727">
        <property type="protein sequence ID" value="BAD39727"/>
        <property type="gene ID" value="STH742"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH742"/>
    <dbReference type="eggNOG" id="COG3063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1234513_0_0_9"/>
    <dbReference type="OMA" id="SSCEAYY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="25750" checksum="E5FBCF0364876445" modified="2004-10-11" version="1">MKTFGLMIAISWLLRTPLGQLLLLLLVLSYLDNRYLGIGAAASAYIHHRRRIASLRYTVQVNPSDVRAMVELGEHYLRSGDPAQAAGYLERALERGEDSARAHYLLGAAWVRVGRGDQGRDLLEKALALQPNVAYGEPYLYLIEEAAARRDDRRIEELVSELRQFESVEILTRAGHLCRSAGLSDLARQLFDEAIRNYQYIPAKMRRRERRWLWRARLGQMQTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR5</accession>
    <name>Q67RR5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome D ubiquinol oxidase subunit II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH643</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome ubiquinol oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194776.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH643"/>
    <dbReference type="EnsemblBacteria" id="BAD39628">
        <property type="protein sequence ID" value="BAD39628"/>
        <property type="gene ID" value="STH643"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH643"/>
    <dbReference type="eggNOG" id="COG1294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049294_0_1_9"/>
    <dbReference type="OMA" id="TAWAYWV"/>
    <dbReference type="OrthoDB" id="761975at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003317">
        <property type="entry name" value="Cyt-d_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43141">
        <property type="entry name" value="PTHR43141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02322">
        <property type="entry name" value="Cyt_bd_oxida_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000267">
        <property type="entry name" value="Cyt_oxidse_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00203">
        <property type="entry name" value="cydB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="342" mass="38156" checksum="96CFD4D3D72DAC03" modified="2004-10-11" version="1">MSHELLQVIWFALWGLLWAIYFGLDGFDLGSGMLAGIFREDEEQHHLIGSLAPIWDGNEVWVITAGGVTFAAFPKLYATMFSSLYLPLFIILFGLMFRAVAIEFYHHMDYDAGWQKIWGWVLSVASFLVAFLFGVAFGNIFQGMLLDAQGYHGTVLSLLNPYGLLTGVLFVVAFLMNGASWLAHKAPDDGFRAKADTFAKRMWPAVLVVAAAWLVYTPFATRLMENYLKVPALFVVPALAVVLLVLTRVYLEKGRHALALLMGSLTVGITVVAAVIGLFPNMFPSRIDPAYSLTAFNSSSSHYTLQFMFWVVVVFLPFVLLYQIWAYRLFGRQGTGSEAGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV1</accession>
    <name>Q67RV1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH607</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH607"/>
    <dbReference type="EnsemblBacteria" id="BAD39592">
        <property type="protein sequence ID" value="BAD39592"/>
        <property type="gene ID" value="STH607"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH607"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_6_9"/>
    <dbReference type="OMA" id="MREWNDY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="2"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="269"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="38948" checksum="F2ADDAD618908EC1" modified="2004-10-11" version="1">MGILAFTVMGAMAEKFNRLIAGGEAYFANRIAVHSTGGALRLNLLGPEDIEVMKRTPGVRHVETHIMMALDETAGFELAPRFLVGINLPNFARAQLIGDESARLRLARGNWWRPGERQVAVLGSAVAHKMKLDVGDTLQARGRDFRVVGVLEETLSLPDGWALIPEEDARELLLEGSSLLQALGLELERVWTNAYALVEPGMGDELTDRLAHSLRRGYLLHSPEQLVKAAGTASSVLNAAILGSGAIAVIVGSLAVVNTMFFAVGERTREIGIKKAIGAGRWAILREFLAESVLIGFLGGLLGLGVAAGLIAVLNGYAAQEGTPVFLLTPRLALGALGFATVLGGAAGAWPAWRAANLDPVEALRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW9</accession>
    <name>Q67RW9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Malate synthase</fullName>
            <ecNumber evidence="2 7">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH589</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH589"/>
    <dbReference type="EnsemblBacteria" id="BAD39574">
        <property type="protein sequence ID" value="BAD39574"/>
        <property type="gene ID" value="STH589"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH589"/>
    <dbReference type="eggNOG" id="COG2225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018928_3_0_9"/>
    <dbReference type="OMA" id="CHKRGAP"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="PTHR42902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="Malate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="SSF51645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="534" mass="61182" checksum="53F9349819612160" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ4</accession>
    <name>Q67RZ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH564"/>
    <dbReference type="EnsemblBacteria" id="BAD39549">
        <property type="protein sequence ID" value="BAD39549"/>
        <property type="gene ID" value="STH564"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH564"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048111_0_2_9"/>
    <dbReference type="OMA" id="NYYLAGE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014258">
        <property type="entry name" value="CAP_domain_YkwD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02909">
        <property type="entry name" value="spore_YkwD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269348" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="43"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="141"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="27890" checksum="5F8CC8374C8FA644" modified="2004-10-11" version="1">MGHRRVTSGKGGESMRINKIKGLAIALAVAASALTFSAPASAATIYVTYQTPVRYTTSNLLNRYVTLVTVRWSSGQTTVKQTVPTQQTTWKQTTSIQTQPSPSKPTTTQTSTPQTSTPTQQTTPQTQTTSAGLTAAEQQMLNLVNQERAKAGLKPLQADAQLTKLARMKSQDMINKGYFSHNSPTYGSPFDMMKAYGVSYRTAGENIAGNQSVQAAHTALMNSSGHRANILNANYTHVGIGIVEGGPYGMMFTQMFVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S10</accession>
    <name>Q67S10_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH548</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH548"/>
    <dbReference type="EnsemblBacteria" id="BAD39533">
        <property type="protein sequence ID" value="BAD39533"/>
        <property type="gene ID" value="STH548"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH548"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049568_0_0_9"/>
    <dbReference type="OMA" id="VEKGQCI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012046">
        <property type="entry name" value="LytTR_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007492">
        <property type="entry name" value="LytTR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04397">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036612">
        <property type="entry name" value="ABC_ATP_LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00850">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50930">
        <property type="entry name" value="HTH_LYTTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="HTH LytTR-type" evidence="4">
        <location>
            <begin position="247"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="37830" checksum="03F460F8B4712937" modified="2004-10-11" version="1">MTTLLEIADLHHPDGLHGLSLTVSAGESLALVGSHGNGKDPLLRLLAGKESPSRGHVRICGCRPDTPEARAKVGFAGATWGLSPYHTVWETLALSAGLWSLPRRRAEEVTAALELAPVAHRLCADLSPGEAARLRIARALLHDPALLVLDEPLGDSDLESAEIIQAALSALAEEGKGILLTTFGHPRTVRAATRVCYLERGRLVGAGQSPAAPDAERRGAARDGSDRADVTGRVPDAGQVPPRVAQIAARRDERVVLFSPAEILYAYAQDKGVYLHTDEGDWTASFTLAELEARLAPHGFFRAHRGYLVNLAHVKEIIPWTRSSFSLRMKDGSDVPLSKHRVAELKELLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S46</accession>
    <name>Q67S46_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="7">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ddl</name>
        <name evidence="11" type="ordered locus">STH512</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194646.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH512"/>
    <dbReference type="EnsemblBacteria" id="BAD39497">
        <property type="protein sequence ID" value="BAD39497"/>
        <property type="gene ID" value="STH512"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH512"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039268_0_0_9"/>
    <dbReference type="OMA" id="NTTPGMT"/>
    <dbReference type="OrthoDB" id="764798at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 7 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="144"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR039102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="366" mass="40059" checksum="2B4D0DD6FA097C87" modified="2004-10-11" version="1">MCAKTRVYLLFGGRSGEHEVSLMSARNVLEALDRSKYEVIPVGITKEGRWLQVGDPLRALTEGVAAAGGRPVGLLGDPSYSGPVPLSDGLRPAAHDGPVVFFPVLHGTYGEDGTIQGLLEMANVPYVGCGVLASSVAMDKGVAKALFRQAGLEVGPYRVYLRRDWERHPEQIRADVSAHLRYPVFVKPANLGSSVGITKVKRPEDLDRAMDEAARFDRRIIVEQGIDAREIEVAVLGNDDPIASVPGEILPGAEFYDYTDKYFSGASQTRIPADLPDDLVEEFRRQAVVAFKAIDGSGMARVDFFLERGTNRIFINEVNTIPGFTNISMYPKLWEASGIGYGELLDRLIQLALERHADKNRNATSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE9</accession>
    <name>LGT_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="1">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lgt</name>
        <name type="ordered locus">STH409</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194543.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SE9"/>
    <dbReference type="SMR" id="Q67SE9"/>
    <dbReference type="STRING" id="292459.STH409"/>
    <dbReference type="EnsemblBacteria" id="BAD39394">
        <property type="protein sequence ID" value="BAD39394"/>
        <property type="gene ID" value="STH409"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH409"/>
    <dbReference type="eggNOG" id="COG0682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013386_1_2_9"/>
    <dbReference type="OMA" id="TDVPWAI"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000172696" description="Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <sequence length="268" mass="29866" checksum="7F74D6B2D7A1D993" modified="2004-10-11" version="1">MQLLAFVDPVAIQIGPLSIRWYGIIIVSAIALSIWLGGRFARDRGLDPAFVDSFAIILVPAGILGARLYEVFVLQWPYYSQHPDKILQIWEGGLAIHGAVLGGAIAAAIYLPMRKQPFWRWADVVGLVLPLAQAIGRWGNFFNQEAYGDPAPDWLVRLMPGWLREGMTISGTVMHPTFLYESVWNLLTFGILLVCHRRRMKTGVVFSLYLVLYNAGRFLIESIRQDSSFIFGRIRVAQLVAAVLAILGLVLLAWFLRRPAESGDSAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK4</accession>
    <name>Q67SK4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thiamine biosynthesis enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH354</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194488.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH354"/>
    <dbReference type="EnsemblBacteria" id="BAD39339">
        <property type="protein sequence ID" value="BAD39339"/>
        <property type="gene ID" value="STH354"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH354"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046249_0_0_9"/>
    <dbReference type="OMA" id="LICAYRL"/>
    <dbReference type="OrthoDB" id="419725at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024007">
        <property type="entry name" value="FeFe-hyd_mat_HydG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034428">
        <property type="entry name" value="ThiH/NoCL/HydG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583">
        <property type="entry name" value="PTHR43583"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43583:SF2">
        <property type="entry name" value="PTHR43583:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00319">
        <property type="entry name" value="Fe_hydrogenase_maturase_(HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03955">
        <property type="entry name" value="rSAM_HydG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="76"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="469" mass="52766" checksum="C8AB8F8CA4913832" modified="2004-10-11" version="1">MPVAAEKASFIDDALLDCLLGQASAPSAARLGQILDKAALAGGLDLEEVADLLMVEDPDHLEALFRTAFEIKQKIYGNRIVLFAPLYVSNLCVNNCRYCGFRRSNDQEVRRRLTQEEVAAEVRALIRMGHKRVLLEAGEDPVHCDIDYIVDCIRTIYGVREGKGNIRRINVNIAATTTEEYRKLKEAQIGTYQLFQETYHRPTYALMHPSGPKHNYDWHTTAHDRAMEAGIDDVGVGVLFGLYDYRYEVLAMLLHVRHLEEAFGVGPHTISVPRLQPAPGVDLHTFPHLVTDDQFKKLVAILRLAVPYTGMIISTRETPEMRAQVLRLGVSQISAGSSVGIGGYSQMTEEQRAATAQFVQGDHRSPDEVIRWLCEEGYLPSYCTACYRQGRTGDRFMALAKTGQIQNLCQPNAILTFEEYLEDYASEATREVGRRTEAANLAQIPSEKVRAETERRLVRIRAGERDLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN4</accession>
    <name>Q67SN4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH324"/>
    <dbReference type="EnsemblBacteria" id="BAD39309">
        <property type="protein sequence ID" value="BAD39309"/>
        <property type="gene ID" value="STH324"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH324"/>
    <dbReference type="eggNOG" id="ENOG502ZXRM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2071963_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="118" mass="12678" checksum="69C2230EE45FB0C2" modified="2004-10-11" version="1">MAVMSDLWGKTPLRVVSMARPKTQVGLTEKPVIPAPGASGPQSILMGTGRLRRRREIRGLATPAEYDQLEADYEALVVRTVTLADGMVMRAMIASLEADEVEGTGGSLLSYTMTLVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN6</accession>
    <name>Q67SN6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="4 9">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH322</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4 9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH322"/>
    <dbReference type="EnsemblBacteria" id="BAD39307">
        <property type="protein sequence ID" value="BAD39307"/>
        <property type="gene ID" value="STH322"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH322"/>
    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_3_0_9"/>
    <dbReference type="OMA" id="LEIWHID"/>
    <dbReference type="OrthoDB" id="357568at2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="NrdE_NrdA"/>
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    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
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    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
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        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIBORED_LARGE" evidence="11">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="782" mass="88804" checksum="FF0F3FE73395DA62" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX9</accession>
    <name>Q67SX9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH229</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
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        <property type="protein sequence ID" value="BAD39214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="sth:STH229"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_9"/>
    <dbReference type="OMA" id="IWLSIMS"/>
    <dbReference type="OrthoDB" id="562287at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="255"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="419" mass="43493" checksum="700895DFCDF1D81D" modified="2004-10-11" version="1">MNLDGWTVGIDSAWTMVAASLVFFMQAGFAMVEAGFTRSKNAVNIVLKNLMDFVVASIAFWAVGFGLMFGADAGGLVGLGGFGSPESRFLHLGLAIPFFVFLWFQTTFAGTAATIVSGAMAERTKFSAYLLFSAAITLLVYPVGGHWIWGGGWLAQLGFVDFAGSTVVHSVGAWAALAGLLLLGPRIDRYDKQGRPRKIVGHNLVLAALGVFILWFGWFGFNPGSTLSATDPNIGRIAVTTNLGAAAGALAALLSAYLRTRTWEAEAALNGALAGLVGITAGCAFVSPAASILIGAVAGMLLAPGTALLERFRIDDAVGAIPVHGFAGLWGTLAVGLFHETQGLLVGGGPQLLAIQALGAAAVFAYAFAVCLLIFATIKATVGLRVSQEEELSGLDTEHGARAYPDFQPAAAHGYTGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ1</accession>
    <name>Q67SZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194352.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH217"/>
    <dbReference type="EnsemblBacteria" id="BAD39202">
        <property type="protein sequence ID" value="BAD39202"/>
        <property type="gene ID" value="STH217"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH217"/>
    <dbReference type="eggNOG" id="ENOG502Z8T8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031634_0_0_9"/>
    <dbReference type="OMA" id="KWQDVTQ"/>
    <dbReference type="OrthoDB" id="1979475at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031599">
        <property type="entry name" value="ABC_tran_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16949">
        <property type="entry name" value="ABC_tran_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="518"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="551" mass="59860" checksum="9F71A34F50431B1E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ7</accession>
    <name>Q67SZ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulatory protein, repressor-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194346.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH211"/>
    <dbReference type="EnsemblBacteria" id="BAD39196">
        <property type="protein sequence ID" value="BAD39196"/>
        <property type="gene ID" value="STH211"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH211"/>
    <dbReference type="eggNOG" id="COG3327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067515_2_0_9"/>
    <dbReference type="OMA" id="LLIHEYR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012906">
        <property type="entry name" value="PaaX-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013225">
        <property type="entry name" value="PaaX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011965">
        <property type="entry name" value="PaaX_trns_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07848">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08223">
        <property type="entry name" value="PaaX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020623">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PaaX" evidence="2">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PaaX_C" evidence="3">
        <location>
            <begin position="167"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="285" mass="33295" checksum="CCC1C5274BA21B21" modified="2004-10-11" version="1">MKARSLLFNLWGDYIQHVGGEAWASTLAAWVRPFGVSEAALRQALSRMARQGWLEVRKVGNRTCYALSAAGRRRIAEASRRVYDGRDVDWDGRWRVLVYSVPEALRNRRNDLRRELIWTGFAHLSPGTWISPNPLEDSVRELLRRYGLEPYATLFVAPYAEPWSAPDLVRRCWDLEAIQASYDRFIARWEPRLEASSRLHSDEERFVEQIRLVHDYRKFLFVDPGLPRRLLPDTWRGHDARRLFQAYYARLRPGALRFLERHFEPTQAHDGGGEDRGVREHPGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ9</accession>
    <name>Q67SZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194344.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39194">
        <property type="protein sequence ID" value="BAD39194"/>
        <property type="gene ID" value="STH209"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH209"/>
    <dbReference type="HOGENOM" id="CLU_2482152_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="87" mass="10033" checksum="3ACE0B8E40874A31" modified="2004-10-11" version="1">MFMCKYCLEQFEDERLAYILFPESRKNHPAADAFALKFCSRAHLVAFLQHISHQHQPYSLTRVAGNSRETFPAAPPLDLLHQMSQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T16</accession>
    <name>Q67T16_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Diadenylate cyclase</fullName>
            <shortName evidence="10">DAC</shortName>
            <ecNumber evidence="10">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="10">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dacA</name>
        <name evidence="13" type="ordered locus">STH192</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a second messenger used to regulate differing processes in different bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably a homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate cyclase family. DacA/CdaA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH192"/>
    <dbReference type="EnsemblBacteria" id="BAD39177">
        <property type="protein sequence ID" value="BAD39177"/>
        <property type="gene ID" value="STH192"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH192"/>
    <dbReference type="eggNOG" id="COG1624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038561_0_1_9"/>
    <dbReference type="OMA" id="ILWQGEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01499">
        <property type="entry name" value="DacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014046">
        <property type="entry name" value="C-di-AMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034701">
        <property type="entry name" value="CdaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045585">
        <property type="entry name" value="CdaA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19293">
        <property type="entry name" value="CdaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004793">
        <property type="entry name" value="UCP004793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00159">
        <property type="entry name" value="TIGR00159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="DAC" evidence="12">
        <location>
            <begin position="95"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="288" mass="31771" checksum="B9B1F7CE2BC25737" modified="2004-10-11" version="1">MAPVDLTSVVNFLAGLGVRSLWDVFRTVLDISLVAYLFYKLFSLIRGTRAVPLINGVIILVVALQVAEALRFYTIQFILENVFIAASVALPIIFQPELRRALEHLGRGRLIKTSFRVDEFGDEERVRMIDQVVRAAEILGRTKTGALIVIERETGLNEFIDSGIKLDALVSAELLVNIFVEKTPLHDGAAVIRQNRVAAAACWLPLAEATVLPHELGTRHRAAVGITEQSDCVVVVVSEETGVISVAVQRQLTRNLDEKSLRELLLKLTEGNRRPGPVRGLFNWGASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T64</accession>
    <name>Q67T64_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194279.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH144"/>
    <dbReference type="EnsemblBacteria" id="BAD39129">
        <property type="protein sequence ID" value="BAD39129"/>
        <property type="gene ID" value="STH144"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH144"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_4_9"/>
    <dbReference type="OMA" id="WDSELWN"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269972" evidence="4">
        <location>
            <begin position="23"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="5">
        <location>
            <begin position="97"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="565" mass="62828" checksum="ED9E40CD3FF52992" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB1</accession>
    <name>Q67TB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH97</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194232.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH97"/>
    <dbReference type="EnsemblBacteria" id="BAD39082">
        <property type="protein sequence ID" value="BAD39082"/>
        <property type="gene ID" value="STH97"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH97"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_3_9"/>
    <dbReference type="OMA" id="EWPVGSR"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="26273" checksum="F8C08A269D8347F4" modified="2004-10-11" version="1">MGRFERVSSQRIYQQIVDQITRMVQEGTLRPGDRLPPERQLAEEFGVSRSAVREALSALRMLGLVEARVGEGTFVTQPPDERFISPLALVLTIEQSEAVGRELLELRAALEAESAALAAVRREAEDLAAMEEALGDMERDLQEGRLGAEADWRFHDAVASASGNSLLLQTMRSLSDTMKEALGLYREQLLRIPGMGRTLLQDHLEILEAIRNQDAAAARERMLKHITRVQEHLYG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB6</accession>
    <name>ATPE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpC</name>
        <name type="ordered locus">STH92</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text>F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194227.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TB6"/>
    <dbReference type="SMR" id="Q67TB6"/>
    <dbReference type="STRING" id="292459.STH92"/>
    <dbReference type="EnsemblBacteria" id="BAD39077">
        <property type="protein sequence ID" value="BAD39077"/>
        <property type="gene ID" value="STH92"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH92"/>
    <dbReference type="eggNOG" id="COG0355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084338_1_3_9"/>
    <dbReference type="OMA" id="MGGFAEI"/>
    <dbReference type="OrthoDB" id="1696893at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036794">
        <property type="entry name" value="ATP_F1_dsu/esu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020547">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00401">
        <property type="entry name" value="ATP-synt_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="SSF46604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000188224" description="ATP synthase epsilon chain">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <sequence length="139" mass="15264" checksum="DD5C4E1FCAAEB5E3" modified="2004-10-11" version="1">MADMSLTIITPERTVLRDAPADAVVVPVVDGSMGILKNHAPMVANLRIGVLRYKQDGVYKRVAVTGGFVEVSENRITVLADAAELAESIDVMRAMEAKRRAEARLRDRKANIDRTRAEAALRRAMVRLRAAGALDHDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI1</accession>
    <name>Q67TI1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH27</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194162.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH27"/>
    <dbReference type="EnsemblBacteria" id="BAD39012">
        <property type="protein sequence ID" value="BAD39012"/>
        <property type="gene ID" value="STH27"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH27"/>
    <dbReference type="eggNOG" id="COG1765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114057_1_2_9"/>
    <dbReference type="OMA" id="GPRPMEM"/>
    <dbReference type="OrthoDB" id="1430018at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="15742" checksum="826BA90C4CFBE29B" modified="2004-10-11" version="1">MRQALVRWAGGIAFEAQLGSGHTIVMDASREVGGEDRGPRPGELLLAALGGCTGMDIVSILQKMRVDFDRVEVTVEAEAREEYPKYYDRFRVIYRVFGGDVPADKVKRAVQLSEERYCSVGALFSHGAEITHRIEINGEPVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J30</accession>
    <name>YIDD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative membrane protein insertion efficiency factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH3339</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in insertion of integral membrane proteins into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0161 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197450.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J30"/>
    <dbReference type="STRING" id="292459.STH3339"/>
    <dbReference type="PRIDE" id="Q67J30"/>
    <dbReference type="EnsemblBacteria" id="BAD42320">
        <property type="protein sequence ID" value="BAD42320"/>
        <property type="gene ID" value="STH3339"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3339"/>
    <dbReference type="eggNOG" id="COG0759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144811_6_0_9"/>
    <dbReference type="OMA" id="QPTCSHY"/>
    <dbReference type="OrthoDB" id="1912220at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00386">
        <property type="entry name" value="UPF0161_YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000253184" description="Putative membrane protein insertion efficiency factor">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00386"/>
        </source>
    </evidence>
    <sequence length="70" mass="8021" checksum="03F9F514BD1C4846" modified="2004-10-11" version="1">MTRLLMLLVRFYQRYLSPLKGGPTCRFTPSCSQYAYEALAKYGAIKGTWLAVRRVLRCHPFHPGGYDPVP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J33</accession>
    <name>MNME_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="1">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmE</name>
        <name evidence="1" type="synonym">trmE</name>
        <name type="ordered locus">STH3336</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD42317" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42317.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197447.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J33"/>
    <dbReference type="SMR" id="Q67J33"/>
    <dbReference type="STRING" id="292459.STH3336"/>
    <dbReference type="EnsemblBacteria" id="BAD42317">
        <property type="protein sequence ID" value="BAD42317"/>
        <property type="gene ID" value="STH3336"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3336"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019624_4_1_9"/>
    <dbReference type="OMA" id="CEIQCHG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000345914" description="tRNA modification GTPase MnmE">
        <location>
            <begin position="1"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="TrmE-type G">
        <location>
            <begin position="221"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="231"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="250"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="275"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="457" mass="48885" checksum="9637D33C2D92D0D8" modified="2008-09-02" version="2">MGEETIAAIATGAGEGGIGIVRISGADALQVAERIFRPRRGRPLGCRRSHTVTYGWVVTPGGDRIDEALALVMRGPHSYTGEDVVELQCHGGQLAVRRVLEQALQAGARLAEPGEFTRRAFLNGRLDLSQAEAVVDLIRAKTDRAMAAAVAHLRGSLRQAIGRIRERLMEMMAHLEADIDFPELELEVQTREEVAAGCAWCLGEVERLLGGARTGRILREGLRAVLAGRPNVGKSSLLNRLVRENRAIVTPIPGTTRDVIAEWVELGGVPVQLFDTAGLRPTDDPVERIGVARTHEALAQAHLVLVVVDAAAGLGPEDREWISQLPQGAARVGVANKIDLNPAFELSALREALGGAPVVGVSAETGEGFDALEAEVARVAGAFDASEELLVNARQAEAIRRARNHLRDAQATLESGLGDELVAIDLRAAWMALGEVTGETAGEELLDQIFSRFCIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J46</accession>
    <name>Q67J46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3323</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197434.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3323"/>
    <dbReference type="EnsemblBacteria" id="BAD42304">
        <property type="protein sequence ID" value="BAD42304"/>
        <property type="gene ID" value="STH3323"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3323"/>
    <dbReference type="HOGENOM" id="CLU_1184551_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003825">
        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="24335" checksum="EE0DBFEF7DF651B7" modified="2004-10-11" version="1">MDLSAMSPLDWVVVVILLLAVGAGWVRGIVRVLLGFLSFLAAVMIAGRATGPVVAWLDGMWNLTGRIADGILDRSVDAGGALSASLDQVAIPQPYKAALVQDVARATAESGEASALELAAQQIAEGAATAVCFVLLVILLAAALRWLGSLFADVVQSLPIVGLSDRLLGAAALGAAAVLALNLVLVWVMPTLSVFGLRGLGELVSQSVTPPYLIQAFEWMRRLVIGGGLRLWNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J65</accession>
    <name>MURA3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine 1-carboxyvinyltransferase 3</fullName>
            <ecNumber evidence="1">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enoylpyruvate transferase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylglucosamine enolpyruvyl transferase 3</fullName>
            <shortName evidence="1">EPT 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murA3</name>
        <name type="ordered locus">STH3304</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197416.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J65"/>
    <dbReference type="SMR" id="Q67J65"/>
    <dbReference type="STRING" id="292459.STH3304"/>
    <dbReference type="EnsemblBacteria" id="BAD42285">
        <property type="protein sequence ID" value="BAD42285"/>
        <property type="gene ID" value="STH3304"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3304"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_9"/>
    <dbReference type="OrthoDB" id="537477at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231286" description="UDP-N-acetylglucosamine 1-carboxyvinyltransferase 3">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <sequence length="424" mass="45497" checksum="004C2B0B0C064DAA" modified="2004-10-11" version="1">MTTLRIRGGRPLQGTIRVGGRKNATLPLIAATLLADGTSRLENVPQIHDVMVYRKLLTGLGARVDWDPVHGILTVHTGGVRPGEPDYHLASSIRASYYLLGVMLARYGEASVPMPGGDNIGHRPVDQHFKGLSALGAEIWMDRGIIRARARRLRGAHVYLDIISVGATIQVMLAASLAEGTTVIQNCAREPHVVAVANFINACGGKVTGAGTDTIRVRGVDRLVGATQTVIPDDIEAGTWMMAAAMTQGDVTLQNVIPTHITPIMAKLREAGVEVHELGDAVRVVGRERPKAINVKTLPYPGFPTDAQSQMTALLSLAEGTSYITETLYEDRFRFVPELVRMGAQIRVEGRTAIVKGVEQLYGAPVEATDIRAGAALVIAGLAAEGETTIYGMEHVQRGYEKLEQKLLALGADVQMERQGAAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD4</accession>
    <name>Q67JD4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stage V sprulation protein T</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spoVT</name>
        <name evidence="3" type="ordered locus">STH3234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3234"/>
    <dbReference type="EnsemblBacteria" id="BAD42216">
        <property type="protein sequence ID" value="BAD42216"/>
        <property type="gene ID" value="STH3234"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3234"/>
    <dbReference type="eggNOG" id="COG2002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105826_0_0_9"/>
    <dbReference type="OMA" id="IGEMGAF"/>
    <dbReference type="OrthoDB" id="1942483at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014213">
        <property type="entry name" value="SpoVT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026579">
        <property type="entry name" value="Spore_V_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02851">
        <property type="entry name" value="spore_V_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="2">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="186" mass="20417" checksum="EC8E08E5A437269F" modified="2004-10-11" version="1">MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFVDRDGEVILKKYSPIGELGEFAKEYADSLYEAIGHTALIADRDTIIAVAGAPKKEFLNKPIGSIVERAMEERRSIVVSRAGETRQRGTIIGDDEDENRIPVYVVAPIVAGGDPIGAVIICSREADAVMTETEIKLAETAAGFLAKQMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE5</accession>
    <name>Q67JE5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mrr_cat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3223"/>
    <dbReference type="EnsemblBacteria" id="BAD42205">
        <property type="protein sequence ID" value="BAD42205"/>
        <property type="gene ID" value="STH3223"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3223"/>
    <dbReference type="eggNOG" id="COG1787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101688_1_0_9"/>
    <dbReference type="OMA" id="LIDRDEW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mrr_cat" evidence="1">
        <location>
            <begin position="51"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="182" mass="20591" checksum="63063E1CA94D1010" modified="2004-10-11" version="1">MLWELRWALLLYFGGLLVCYKVIDSIRYWIGEWRREKAEARFRGADLAAVDQMSGQEFLQYLAYLFRSAGFTVQFTPPVGNYGVDLVLTHPTTEQRIAVRAHRADDPVDPIPVMTVESSRLLYGCERAMVVTSGEFTEEAREQGEAANVILVDRTRLQEMMVLTSAPHTALFGFSGHQGLTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ0</accession>
    <name>MRNC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mini-ribonuclease 3</fullName>
            <shortName evidence="1">Mini-3</shortName>
            <shortName evidence="1">Mini-RNase 3</shortName>
            <ecNumber evidence="1">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Mini-RNase III</fullName>
            <shortName evidence="1">Mini-III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mrnC</name>
        <name type="ordered locus">STH3118</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MrnC RNase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197232.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JQ0"/>
    <dbReference type="SMR" id="Q67JQ0"/>
    <dbReference type="STRING" id="292459.STH3118"/>
    <dbReference type="EnsemblBacteria" id="BAD42100">
        <property type="protein sequence ID" value="BAD42100"/>
        <property type="gene ID" value="STH3118"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3118"/>
    <dbReference type="eggNOG" id="COG1939">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091169_2_1_9"/>
    <dbReference type="OMA" id="AYMGDAI"/>
    <dbReference type="OrthoDB" id="1939897at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01468">
        <property type="entry name" value="RNase_Mini_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008226">
        <property type="entry name" value="Mini3_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005520">
        <property type="entry name" value="UCP005520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000415993" description="Mini-ribonuclease 3">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="130" mass="14382" checksum="EE8A20B945041DFB" modified="2004-10-11" version="1">MRLANPLTLPPLTLAYLGDALYEAFVRERLLERGYVRVNDLHRHALRYVQATAQAAILHHLMPALTEQEQDVVRRGRNAKGHGAPKSADPAEYAASTGFEALVGYLYLAGQAERLSEVLQAAADFIERGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ4</accession>
    <name>Q67JQ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3114</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197228.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3114"/>
    <dbReference type="EnsemblBacteria" id="BAD42096">
        <property type="protein sequence ID" value="BAD42096"/>
        <property type="gene ID" value="STH3114"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3114"/>
    <dbReference type="eggNOG" id="COG2720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075501_0_0_9"/>
    <dbReference type="OrthoDB" id="967747at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011098">
        <property type="entry name" value="G5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007391">
        <property type="entry name" value="Vancomycin_resist_VanW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07501">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04294">
        <property type="entry name" value="VanW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01208">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51109">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269233" description="G5 domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="G5" evidence="3">
        <location>
            <begin position="196"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="30422" checksum="376C56D2DF6BA955" modified="2004-10-11" version="1">MAALLLITALWIACLAPVRRAAAGEPRTPAPPDLPVPEQAYRFVLARFQTPIVNLAPGRMHNIALAASRIDGRVLQPGEVFSFNDVVGPRDPAHGWALADEIYQGEYVPGYGGGICQVTSTLYNAALLAGLTIVERYHHDRPLQYISPGRDATVVWGQLDFRFRNSLDRPVQIRARVTPEDPPRVEVTLHGDRPSAGDPIRLEEEYVRFTPPELVEVEDPGLPRGRRVVEDEGYFGVEVRIYRVMGLGPEARRELVSHDVYPPRPGRVRVGTAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU5</accession>
    <name>RL23_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplW</name>
        <name type="ordered locus">STH3073</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU5"/>
    <dbReference type="SMR" id="Q67JU5"/>
    <dbReference type="STRING" id="292459.STH3073"/>
    <dbReference type="EnsemblBacteria" id="BAD42055">
        <property type="protein sequence ID" value="BAD42055"/>
        <property type="gene ID" value="STH3073"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3073"/>
    <dbReference type="eggNOG" id="COG0089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_3_2_9"/>
    <dbReference type="OMA" id="FEVDHRA"/>
    <dbReference type="OrthoDB" id="1978865at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000272854" description="50S ribosomal protein L23">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <sequence length="94" mass="10808" checksum="AC8F023B88274C77" modified="2004-10-11" version="1">MEARDIILKPLITEKSVAKMSEGKYAFKVRLDANKTQIKQAIEEIFGVTVVRVNTMRVRGKLRRQGKYIGRRSDWKKAIVQLKEGDSIKVFEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX8</accession>
    <name>Q67JX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197154.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3039"/>
    <dbReference type="EnsemblBacteria" id="BAD42022">
        <property type="protein sequence ID" value="BAD42022"/>
        <property type="gene ID" value="STH3039"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3039"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014322_7_0_9"/>
    <dbReference type="OMA" id="MANERKA"/>
    <dbReference type="OrthoDB" id="1184688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MurNAc-LAA" evidence="2">
        <location>
            <begin position="118"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="27712" checksum="8DCA699043E8A80A" modified="2004-10-11" version="1">MRKFPLPQAVLLGLVAAVLVAATWPLWKGLRASREGGEPQAPEPAPGPLAGLVIVVDPGHGGWDPGAVVAGVKEKDLTLQVAHVLKEILEARGARVVLTRTDDTHYSRTVREDLRQRVALVGEHQADLFLSLHANQDRCHCWGAQTFYQKGGSAEGKALAISIQNRLRERTDTTRYALPGDYFVLRTTEVPAVIVEMGFLSNAAERGRMQQPEYQRTIAEAIADGVVDYREYISRHPGTAPENPADPGEASPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ5</accession>
    <name>Q67JZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3022"/>
    <dbReference type="EnsemblBacteria" id="BAD42005">
        <property type="protein sequence ID" value="BAD42005"/>
        <property type="gene ID" value="STH3022"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3022"/>
    <dbReference type="eggNOG" id="COG4120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067296_0_0_9"/>
    <dbReference type="OMA" id="FSANTDW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="291" mass="30461" checksum="3AE3D3972D1ED7F3" modified="2004-10-11" version="1">MELILNFLLPVLQQGLVWGLVALGVWMTFRVVNVPDLTVDGTLTTGAATAARLLTMGVHPVTATLAGFATGAAGGAITGLIHTRLRVQPLLASIITMTGLYSINLRIMGRSNIALFTLKTLVPDNSWARLALFAGVAALLVLLMNLFFRTELGLALRATGDNDAMVRMLGVSSDSMRLLAFTLSNAVVGLGGALVAQYSGFADAQMGIGTIVASLAALIIGEALFGSPTVFRATLSALLGSIIYRAIFALALRAGFQASDLKLVTAALVVIALSIPQIKRTIRLPEGSEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD9</accession>
    <name>Q67KD9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fur family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196993.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2876"/>
    <dbReference type="EnsemblBacteria" id="BAD41859">
        <property type="protein sequence ID" value="BAD41859"/>
        <property type="gene ID" value="STH2876"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2876"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_4_2_9"/>
    <dbReference type="OMA" id="DHPHADT"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="156" mass="16850" checksum="C3BDB503959D7D02" modified="2004-10-11" version="1">MTESGHQVAEQHREDPFTAGLRAAGLKLTAQRAAICAALADMAGKQHPTVQEIFEAARKHHPGVSLATVYNTLTVLKERGLVYELGPDAAGAVHYELDVDTHVNVICIRCKQVIDLHHISTGLEEMVQAETGYRLVGAQMLYYGICPACARDGAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF1</accession>
    <name>Q67KF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196981.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2862"/>
    <dbReference type="EnsemblBacteria" id="BAD41847">
        <property type="protein sequence ID" value="BAD41847"/>
        <property type="gene ID" value="STH2862"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2862"/>
    <dbReference type="eggNOG" id="COG0351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_0_9"/>
    <dbReference type="OMA" id="KDEVGYA"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="2">
        <location>
            <begin position="11"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="28532" checksum="69BFB1975DD4A411" modified="2004-10-11" version="1">MRKALTIAGSDTSGGAGIQADLKTFQELGVYGMSALNVIVTMDPNGWSHQVFPLDLELLERQLITAFEGVGVDAVKTGMLASAAVVELVARKLDQYGAERVVIDPVMVCKGTDEVLMPDHAAAIRELLIPRALVITPNLFEAAQLAQRPPIRTLDDMKEAAARIAEAGAKNVLIKGGSKLEGVGKAVDLFYDGKTFTLLESEKVTHGYTHGAGCTTAAAITAELAKGASVADAVRTAKRFVSAAIRHGFRLNQYVGPVMHGALRLHGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI5</accession>
    <name>Q67KI5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich cell wall protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41813">
        <property type="protein sequence ID" value="BAD41813"/>
        <property type="gene ID" value="STH2828"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2828"/>
    <dbReference type="eggNOG" id="ENOG50314RN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1293794_0_0_9"/>
    <dbReference type="OMA" id="GCSISMG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="22271" checksum="70D041C923305B7E" modified="2004-10-11" version="1">MGRAGARPTVASAPRRPPAPDALRINALPLVNAKGKPVRWPVGGGLRTVRARWAAMAFCALLLSGCMAQQQPQQSPEPSSEQVRKAVRESLSEPEMKDYIRLQVKEQAGVEILEMALDTQEGKDALTDAVKEVMGSPTGEQLIQQKLAEMLEDPIVQQQLQQAIRETLQDILSKGTQDSRRGGGQGGGQGGGQGGGQGGGQSGGQQGGGGGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL7</accession>
    <name>Q67KL7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196915.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2796"/>
    <dbReference type="EnsemblBacteria" id="BAD41781">
        <property type="protein sequence ID" value="BAD41781"/>
        <property type="gene ID" value="STH2796"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2796"/>
    <dbReference type="eggNOG" id="COG1694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1966152_0_0_9"/>
    <dbReference type="OrthoDB" id="1760741at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="30"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="125" mass="14136" checksum="FAD25BCA7C5E63C5" modified="2004-10-11" version="1">MFPNAMRTIADFQAFHRWLDEQKGWGPDLKLNMVLLAGEVGEVANELRNIFWRASLLEPEMGEEAAREAALAEYRENLGFELADCLAYIFKIANNAGIDLEAAYKAKMAKNVQRQWTAPPPGNHQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP0</accession>
    <name>NUOH2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit H 2</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit H 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit H 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoH2</name>
        <name type="ordered locus">STH2773</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196892.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KP0"/>
    <dbReference type="SMR" id="Q67KP0"/>
    <dbReference type="STRING" id="292459.STH2773"/>
    <dbReference type="EnsemblBacteria" id="BAD41758">
        <property type="protein sequence ID" value="BAD41758"/>
        <property type="gene ID" value="STH2773"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2773"/>
    <dbReference type="eggNOG" id="COG1005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_9"/>
    <dbReference type="OMA" id="WSGWASN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="PTHR11432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000244959" description="NADH-quinone oxidoreductase subunit H 2">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01350"/>
        </source>
    </evidence>
    <sequence length="394" mass="44177" checksum="F41FE09B95648AD3" modified="2004-10-11" version="1">MMEWWNSLSPTVQVWLGGVLKASLILVWGLINFYLCLMLERKLSAWMQNRVGPWRVGLWGWIQPVADLIKLWVKEYIRPNNVDKWLYLIAPFIGFVSANLVWLIVPFGDKLIATDFEIGIIFIAAVMGYDVIATFMAGWGSNNKYSMLGAMRGAAQLISYEVTMVMAVIGVVMMAGSLRLSDIVLAQQQRGFLGWFLFPQIIGFIVYLIASLAELNRAPFDLAEAEQELVAGHHTEYSGFRWAMFMLAEYIHLAAWSAIAATLFLGGWSGPTLGQVAAGLGNVLNGFGAFTNPVGTAVLNWGLAISGSTILNWVAGVFWLVLKTYFFVFLAMWIRWTLPRVRIDQLMDLGWKFLLPVSMFNIFLTGTLRYLAVAFDGVPISIGSFTLRLLGWWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ2</accession>
    <name>Q67KQ2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vitamin B12-dependent ribonucleotide reductase</fullName>
            <ecNumber evidence="11">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">STH2760</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the ribonucleoside diphosphate reductase class-2 family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="2"/>
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        </location>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022634"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00317"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAD41745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="776" mass="84713" checksum="26C71358B56B88C2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU9</accession>
    <name>Q67KU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
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            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196833.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2712"/>
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        <property type="gene ID" value="STH2712"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2712"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="13"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="178"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="361" mass="40464" checksum="3A078B85E0AAC290" modified="2004-10-11" version="1">MRILYVITLAEKGGAQVHLLDLLRNFSRTAEVHLAVGERGFLTDEAARLGIPIHLIEGLVRPVNPAKDFLAARALVRLLRRIRPDLLHAHSSKAGQVGRLAARLAGVPAVYTVHGWAFADGVPLWRRCVALPAERLAAHWSRYMITVSDADRLLASRYRFPTDRMFVVHNGVPDVVQRAQVGRADLPPHIVMVARFAPQKDQALLLKALSRVNVPFTLSFVGEGETQREHRQLAHQLGMMDRVRFLGSRHDIADILADAQIFVLTSNWEGFPITILEAMRAGLPVIASDVGGVREAVLHGRTGYLVARGDVDALQRYLTTLLTDPQLREKLGNEARKTYEQRFTLDRMLDQTRRIYELIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW1</accession>
    <name>Q67KW1_SYMTH</name>
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        <submittedName>
            <fullName evidence="4">O-antigen biosynthesis-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2700"/>
    <dbReference type="EnsemblBacteria" id="BAD41685">
        <property type="protein sequence ID" value="BAD41685"/>
        <property type="gene ID" value="STH2700"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2700"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_394779_0_0_9"/>
    <dbReference type="OMA" id="FQTWVEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="162"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="698" mass="75843" checksum="7EDDF904F7656D1A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L51</accession>
    <name>Q67L51_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196732.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2610"/>
    <dbReference type="EnsemblBacteria" id="BAD41595">
        <property type="protein sequence ID" value="BAD41595"/>
        <property type="gene ID" value="STH2610"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2610"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_874146_0_0_9"/>
    <dbReference type="OMA" id="NPERIDQ"/>
    <dbReference type="OrthoDB" id="1617768at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="204"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="35524" checksum="391BEEA9FB1CBA81" modified="2004-10-11" version="1">MTDHDRLRQLIQFRYSPLLDLALSLLVIQNPERFGTAAPWEQRVLERLPPGLLERLHGHAQRTDLFALALELEESAPLPTPDALRGLADRQPELAADLLAYWEAISPEIGARVGLLTESIQREAALLAQIDPVSFISRFSDRVGVAGDGDALILHWGKGMRVPLGDLSRILFVPSAFSPRRLMFYRLDRTQIFFYNPEHPAASQPEEAPESLLLGFSALADATRFKLLRLIAQERLPAQEMAKRLGLNESTVSRHLRLLVEAGLVGRAGQEGKFILYELNPERIDQLAAQAKAYLGRDSDDGVDGDLALQNRNRTLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L93</accession>
    <name>Q67L93_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196690.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2568"/>
    <dbReference type="EnsemblBacteria" id="BAD41553">
        <property type="protein sequence ID" value="BAD41553"/>
        <property type="gene ID" value="STH2568"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2568"/>
    <dbReference type="HOGENOM" id="CLU_2703513_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="73" mass="7865" checksum="EB07D4BAD5527987" modified="2004-10-11" version="1">MGVNELILWWVLFLIANAILAAATGMLVKAGPFARVRVPALLSTFLFICGMTLFLVAGKQNLLQETIAAIFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF2</accession>
    <name>Q67LF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41494">
        <property type="protein sequence ID" value="BAD41494"/>
        <property type="gene ID" value="STH2509"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2509"/>
    <dbReference type="HOGENOM" id="CLU_1601850_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="166" mass="18693" checksum="89028F847019F2CD" modified="2004-10-11" version="1">MEYLLTGRDPQRDFEGALRRLPPEDRARLYFASPLTRLRLVLRFLMSEYPAEFPEETLAERLCLSVEELRRLAGTGELTLSDDHVAAMAERLEQLTAIPVHWFMAGFNGELSPCIPPERLAAYARIVEKAAVAGIRPDVLDMAIDLLILQHNELAADHQAAARRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH4</accession>
    <name>Q67LH4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TetR/AcrR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2487</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196610.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2487"/>
    <dbReference type="EnsemblBacteria" id="BAD41472">
        <property type="protein sequence ID" value="BAD41472"/>
        <property type="gene ID" value="STH2487"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2487"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_4_9"/>
    <dbReference type="OMA" id="ARQYLSM"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041490">
        <property type="entry name" value="KstR2_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17932">
        <property type="entry name" value="TetR_C_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="24066" checksum="25ACA2A314D55B3E" modified="2004-10-11" version="1">MGRNRRREAMLQAAIGLFSDKGYHATTVREIAQAVGILPGSLYAHMASKEDLLYEAVVQASERFQEAVAPIAESPGHAGERLRQAMAAHIRVVAESPAAATVFLHEWRALSPQRRAMAVAHRRAYEELLARIIREGVESGVFRPVDEKFVRLLVLSAVNWTYQWYRADGPLSPEQVADQFYAIIAGGLLRNAADPGSAALPASADGEPAPQGPSRVAAGGESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ3</accession>
    <name>Q67LJ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196591.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2468"/>
    <dbReference type="EnsemblBacteria" id="BAD41453">
        <property type="protein sequence ID" value="BAD41453"/>
        <property type="gene ID" value="STH2468"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2468"/>
    <dbReference type="HOGENOM" id="CLU_2453522_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="89" mass="9121" checksum="80C115FADF275E1C" modified="2004-10-11" version="1">MHFGGFDAPDQVFFNPAVDLAPFSKLASLAAFSPAAALAAKSPVLAIFNPAINPRAALLAFNPAAAPTLALTGGLGGKFFKKPFRKGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ9</accession>
    <name>Q67LJ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196585.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2462"/>
    <dbReference type="EnsemblBacteria" id="BAD41447">
        <property type="protein sequence ID" value="BAD41447"/>
        <property type="gene ID" value="STH2462"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2462"/>
    <dbReference type="HOGENOM" id="CLU_1509866_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="19505" checksum="5F5E5BA25C10DB6A" modified="2004-10-11" version="1">MSGDRSSLRPEGGGASVGAVPRRWIVVACAVLAALAAGIVWVYTHPPLQSTGMHTRVDDQRMPVAYGFDLENTGRWPITLVAVKVDGQEILPPFAIAVSNFTEGHLAGSAAIALETYGHKLTTGPVYGWKMQPERYTGHARYAVRLGTRGLPPAHEKIILHHRYLGLPLRHDLTPWIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL8</accession>
    <name>Q67LL8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secD</name>
        <name evidence="10" type="ordered locus">STH2443</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecF. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196566.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2443"/>
    <dbReference type="EnsemblBacteria" id="BAD41428">
        <property type="protein sequence ID" value="BAD41428"/>
        <property type="gene ID" value="STH2443"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2443"/>
    <dbReference type="eggNOG" id="COG0342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_4_2_9"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="OrthoDB" id="121331at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_B">
        <property type="entry name" value="SecD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005791">
        <property type="entry name" value="SecD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01129">
        <property type="entry name" value="secD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="416" mass="44108" checksum="CC06F0C06347F5AB" modified="2004-10-11" version="1">MRGGWNALVLALILVVVGGVIGFYLTPHPQTGSARILQHMKLGLDLQGGAHIVLEGVESELGPVTPDTIQAARQVIENRINALGVSEPIVQVDGQNRIIVEIAGATDIDQAREVIGKTAVLKFIDPEGNEVLTGNDVEKAGVAQNPSGSGFIVTLKLKGEGPDKFYEATRKYVGKPIFILLDDEIISAPMVQEPIPGGEATITGNFTVEEAGTLANLINGGALPVKLVEVEARMVSATLGADSVAKSLRAGWIGIVGVLLFMILLYRIPGLLADIALGVYAILNMVVLLAIDAVFTLPGIAGLLLGIAMAVDGNVIIFERIKEELRKGKGLRSGIDAGFHRAYVTVLDSQITTAIAAAILWYLGTGPVRGFAVTLFVGTVLSIFTAITFTRWLVKLAVDTGWFNKRTLFGVKEVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ8</accession>
    <name>Q67LQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Threonine aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2403</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196526.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2403"/>
    <dbReference type="EnsemblBacteria" id="BAD41388">
        <property type="protein sequence ID" value="BAD41388"/>
        <property type="gene ID" value="STH2403"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2403"/>
    <dbReference type="eggNOG" id="COG2008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029381_0_4_9"/>
    <dbReference type="OMA" id="NKGGGAC"/>
    <dbReference type="OrthoDB" id="1124266at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="PTHR48097:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_elim_lyase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="36670" checksum="6DD339ADBF41C24F" modified="2004-10-11" version="1">MRAIDLRSDTVTKPTPEMRRAMYEAEVGDDVYGEDPTINRLEERAAELLGKEAALFVPSGTMGNQVAVLTHTRRGQEVIVEAESHVYLYEVGGIAALSSCQVKTIPGVRGAMDPAAVEAAIRADNVHFPRTGLVCVENTHNRSGGCVLPPENVAAVAAVAHRHGIPVHMDGARIFNAAVALGRPAAELVAPVDSVMFCLSKGLAAPVGSLLVGSREFIAEARRYRKLLGGGMRQAGVLAAAGLVALEMMIDRLADDHANARRLAEGIAGIPGLRVDLETVQTNMVVVEIVDERWTAGAFVQALRTQGVLCNDTGPRSVRLVTHKDVTAADVELALDAIARTVKAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU6</accession>
    <name>Q67LU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196488.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2365"/>
    <dbReference type="EnsemblBacteria" id="BAD41350">
        <property type="protein sequence ID" value="BAD41350"/>
        <property type="gene ID" value="STH2365"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2365"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1676961_0_0_9"/>
    <dbReference type="OrthoDB" id="1753132at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="15794" checksum="CE7F5B984E004D09" modified="2004-10-11" version="1">MASSIPVQPHPAPGLRRNRAFLTALGGTAVAQLGTSLYPMILPVWVMQTTGSAARVAALSSLGTTVRLLAAPLAGTVADRSDRRAVMALANLARAAIVALLALALASGHGQFWVLAIGSAALVLAGAFFSPACAGAPGGRTPARVGENHGTPRKPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW9</accession>
    <name>Q67LW9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sugar ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2342"/>
    <dbReference type="EnsemblBacteria" id="BAD41327">
        <property type="protein sequence ID" value="BAD41327"/>
        <property type="gene ID" value="STH2342"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2342"/>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_3_2_9"/>
    <dbReference type="OMA" id="QELNNEY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269894" evidence="1">
        <location>
            <begin position="24"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_4" evidence="2">
        <location>
            <begin position="37"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="32977" checksum="44C7DC723BDC9B78" modified="2004-10-11" version="1">MLTAALAVTVSGCGSSQTQTPGSSTPGQQTETKAEYAVVLKVLSSPFWQSMRDGILEEAEKQGIKVDIYAANSEDDVEGQVTLLENAINKGYKAIGVAPISADNLVPAVAEASKKGIYVINIDEKLNLESLKGLGGAVQAFVSTDNKAVGRTGAQYIIDRIGGSGQVAIIEGKAGAASGEDRKAGATEAFLKAGVELVESQPADWDRTKAYDLATNYIAKYPDLKGIYACNDTMAMGALEAVKNAGKDIVVVGTDGNEDAVASVKAGELAATVAQDPAQIGRRGVQLMIEAVKAGKAIDPNAAPAMESVDAILITKDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ6</accession>
    <name>Q67LZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dipeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2315"/>
    <dbReference type="EnsemblBacteria" id="BAD41300">
        <property type="protein sequence ID" value="BAD41300"/>
        <property type="gene ID" value="STH2315"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2315"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_0_9"/>
    <dbReference type="OMA" id="YIAYNVM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="90"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="547" mass="60182" checksum="E7E90E1D4F7AA9A0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M03</accession>
    <name>Q67M03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative PTS system, IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196431.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2308"/>
    <dbReference type="EnsemblBacteria" id="BAD41293">
        <property type="protein sequence ID" value="BAD41293"/>
        <property type="gene ID" value="STH2308"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2308"/>
    <dbReference type="eggNOG" id="COG3037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031784_0_1_9"/>
    <dbReference type="OMA" id="ARLTPWK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004703">
        <property type="entry name" value="PTS_sugar-sp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03611">
        <property type="entry name" value="EIIC-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="456" mass="47058" checksum="E0EB46217636524C" modified="2004-10-11" version="1">MEFLMGFVSDVLSVPAVLVGLVALLGLLFQKKPASDVISGTLKSILGFVILSGGATMLQGALDYMGPLLEIGFGIRGVVPNNEAIVAIAQQTLGTQTALIMVFGLIANLVFARVTPFKYIFLTGHHTFFMAALLSAVLGTAGLDGWLLVLTGSLILGGLMVLMPALASPYMDQVTGGAGVALGHFGTVGYWLSGTIGKLVGNREDSLEKYQFPKGLQFFRESVIATSLVMFIMFLIASLAAGNAETLRLSGGQNMLVFSLMQAVTFAGGVAVVLYGVRMIINEIVPAFKGFADIVVPDSKPALDCPITFPYAPTSVLVGFIVSFLGGLVSMFIMGLVGLPVIVPGLVPHFFVGGTAGVFGNATGGRRGAIAGAFVNGILISFGAAFLLPTLGALGFANTTFGDADFQLVGILVGGIGNLFKGSPYVIAAVLLAILAAILIVGTVTHKGSRAGQKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M25</accession>
    <name>Q67M25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2285"/>
    <dbReference type="EnsemblBacteria" id="BAD41270">
        <property type="protein sequence ID" value="BAD41270"/>
        <property type="gene ID" value="STH2285"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2285"/>
    <dbReference type="eggNOG" id="COG2433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688726_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269899" evidence="2">
        <location>
            <begin position="28"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="3">
        <location>
            <begin position="206"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="3">
        <location>
            <begin position="293"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="391" mass="45303" checksum="DD34EA4AD96D68B5" modified="2004-10-11" version="1">MRRGHVRRSLAVLVLAALLSAPVGAGAEEELTPEVAEYPAENYEPGPGWPEGWTPPPPPEGMSERMRAEVEQILREHPDLYDRYDWWVWDPLPSWTNEYPWFWREDKMGCGTTLHVYAYPVPDTQLITREFGRSFGWSRLYWKPCRYEPIQSITETRELSEEEKLAEGRRAEYLYWVDKSHPGLGRDDGSESNGNPDYIYIYLNQGDAAKRFDVPAYLDTTVNRVRVPIRFVSEMMGAEVSWDPDGRRVTINFPAITREVPKVVPAPGFDYPDLFDPEEYLPNGHRFAFEERTVSTPERTIILTIDHPVALVDGREVPLDAPPVIRNDRTMVPVRFIAEQMGAKVYWVGAEPIFRLDDGTMSGTYQVHIFTPFFPLYEYPSWYLENRAVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M32</accession>
    <name>Q67M32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2278"/>
    <dbReference type="EnsemblBacteria" id="BAD41263">
        <property type="protein sequence ID" value="BAD41263"/>
        <property type="gene ID" value="STH2278"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2278"/>
    <dbReference type="HOGENOM" id="CLU_070817_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="123"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="28412" checksum="8653F46E7F9B05E5" modified="2004-10-11" version="1">MVARPVYHAVALILQHLHRLLDEVAAERRGRKPRNQPKTGRPRLYSTPSLLLFRIAADLLGWSLRKTLNYAEQEAGAWLRKALGWEQVPHLSVVCRRLHGDDVRRLRTRLWRKLRRRSLRQGDLRVLTIDMTDLPVPLRRKDPDATWGATSKGKFFGYKLHLVISRTGFPLAFRVTPAAIHEMRPAVSLFAEVVKLADQKQKAELTYGVGDSAYDSENLYKFWADVCCGHFLCAPNRRGTPEGEEVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M73</accession>
    <name>Q67M73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2237"/>
    <dbReference type="EnsemblBacteria" id="BAD41222">
        <property type="protein sequence ID" value="BAD41222"/>
        <property type="gene ID" value="STH2237"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2237"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_1_0_9"/>
    <dbReference type="OMA" id="GHKMANT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="35972" checksum="033D39F3C360ED3B" modified="2004-10-11" version="1">MEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRCLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGNAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB6</accession>
    <name>Q67MB6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2192"/>
    <dbReference type="EnsemblBacteria" id="BAD41177">
        <property type="protein sequence ID" value="BAD41177"/>
        <property type="gene ID" value="STH2192"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2192"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_5_9"/>
    <dbReference type="OMA" id="LWSKTRI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="365" mass="39286" checksum="6F4CCEB4DDB02020" modified="2004-10-11" version="1">MITSVTGPLAGYADQEIKGLRLGLEYATGGTMEVLGRPIEVIVEDDTGSPDVGKQKAIKLLEQDGVHILQGSASTPVALAVAEQAKLYEVIYMIDPAAGDQLTTTDFNPYIFRTGGNTTQDALTGGKATVENLGRTIVHLAPDNAFGTSSQDAWNPIFEQYGGTVIRNINAPLDAKDFATYLQQVIELNPEVLMVTWSGGNALKLFQQIGELGLFDRMKVTTSIGDRASLIGMGDAAVGMNGVIRYFYTLPDNPVNDWLVREHIARHGEPPDLFTAGGMAAGIAIVEGIKKAGSTDARDLIPALEGMSFEGPKGTYTFRPEDHQALQPMYFVELVKDPDHDYPIPKLIAELSPEETAPPVNVPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG9</accession>
    <name>Q67MG9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oxygen sensor histidine kinase NreB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogen regulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH2139</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxygen, probably at the conserved histidine residue, and transfers its phosphate group probably to a conserved aspartate residue of NreC. NreB/NreC activates the expression of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2139"/>
    <dbReference type="EnsemblBacteria" id="BAD41124">
        <property type="protein sequence ID" value="BAD41124"/>
        <property type="gene ID" value="STH2139"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2139"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_488265_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="15">
        <location>
            <begin position="343"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="534"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="537"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD41124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="558" mass="60821" checksum="1A6DD7C1E647AD67" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH1</accession>
    <name>Q67MH1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">feoB</name>
        <name evidence="12" type="ordered locus">STH2137</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Probable transporter of a GTP-driven Fe(2+) uptake system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2137"/>
    <dbReference type="EnsemblBacteria" id="BAD41122">
        <property type="protein sequence ID" value="BAD41122"/>
        <property type="gene ID" value="STH2137"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2137"/>
    <dbReference type="eggNOG" id="COG0370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013350_6_0_9"/>
    <dbReference type="OMA" id="CITSFAM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="11">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD41122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="585" mass="63941" checksum="53DF32C7782ED6B0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK9</accession>
    <name>Q67MK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase bacterial subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2099"/>
    <dbReference type="EnsemblBacteria" id="BAD41084">
        <property type="protein sequence ID" value="BAD41084"/>
        <property type="gene ID" value="STH2099"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2099"/>
    <dbReference type="HOGENOM" id="CLU_2620758_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005171">
        <property type="entry name" value="Cyt_c_oxidase_su4_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03626">
        <property type="entry name" value="COX4_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8409" checksum="88537E14F2B6F916" modified="2004-10-11" version="1">MGVILAFITAAEFLILYVRGMSALVVTTLAALSAAKFFLVAAYFMHLRFDPRLLTAIFAVGVTLATLITIAVRFISLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM8</accession>
    <name>Q67MM8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2080"/>
    <dbReference type="EnsemblBacteria" id="BAD41065">
        <property type="protein sequence ID" value="BAD41065"/>
        <property type="gene ID" value="STH2080"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2080"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2048536_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="120" mass="12680" checksum="9A3623E39408E305" modified="2004-10-11" version="1">MGGGWCASTRRDTAETSVSVGSSEGAWTARHGPTGRGPASSPQPPGATVDGGTGMSDVEYVNWAGRIRLEWMGRPAETPPAELITMVHGFCFLDGKLMLVDLRARGWDIPGGHRHPGEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP4</accession>
    <name>Q67MP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196189.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2064"/>
    <dbReference type="EnsemblBacteria" id="BAD41049">
        <property type="protein sequence ID" value="BAD41049"/>
        <property type="gene ID" value="STH2064"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2064"/>
    <dbReference type="eggNOG" id="ENOG50330DY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136838_0_0_9"/>
    <dbReference type="OrthoDB" id="1371293at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="166" mass="18877" checksum="010349321DDC85F9" modified="2004-10-11" version="1">MFSIARFLAACLVMCLVFGVLVFGMLLMNPVSFINSYPPEIQAEYYRSQSKEAARRKLTVIMGIKKAAALTAFLFLFAWMAKWAGADTFGEGLMAVYGYGLVLAVFDTCFLDWVLFPNIRRIRLPGTEHMDREYRQKWFHVKAMLPMLPLFAVGGVAAALIMVWIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ7</accession>
    <name>Q67MQ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2051"/>
    <dbReference type="EnsemblBacteria" id="BAD41036">
        <property type="protein sequence ID" value="BAD41036"/>
        <property type="gene ID" value="STH2051"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2051"/>
    <dbReference type="eggNOG" id="COG2414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_9"/>
    <dbReference type="OMA" id="CAYACKL"/>
    <dbReference type="OrthoDB" id="332680at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFOR_N" evidence="3">
        <location>
            <begin position="8"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="304"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="666" mass="71501" checksum="79AB2C9C88F45B44" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW9</accession>
    <name>Q67MW9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196116.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40974">
        <property type="protein sequence ID" value="BAD40974"/>
        <property type="gene ID" value="STH1989"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1989"/>
    <dbReference type="HOGENOM" id="CLU_2208714_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="11736" checksum="82F0300BBC1866C7" modified="2004-10-11" version="1">MPDIPFFDAAGFFAGKIHEVEQQLIAKKAEREILLANRTSLEQALATLALLAPEHQKAIQQEVASLKEVLHSTERALTSLESEIDMLTSKLAALQAAREMLHVEGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N28</accession>
    <name>Q67N28_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AIRS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196057.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1930"/>
    <dbReference type="EnsemblBacteria" id="BAD40915">
        <property type="protein sequence ID" value="BAD40915"/>
        <property type="gene ID" value="STH1930"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1930"/>
    <dbReference type="eggNOG" id="COG0611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076350_0_0_9"/>
    <dbReference type="OMA" id="IPKVGQE"/>
    <dbReference type="OrthoDB" id="1664800at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="25218" checksum="A91BB8C1BA26C168" modified="2004-10-11" version="1">MRWRDLTLVDLPCGGRLVIACDAAGGIGPKERDVIRVAGYVIGRFTARVALMDLLAAGAQPLHLVNNTCVEPDPTGREILQGICDEAALAGLSADQINGSFEKNIPTVQTGLGVTAIGYLAPGRSLRTARPGDLVVAIGRPKVGAEVRLDDPELPDLPLVRRLAGDPLVHDLLPVGSRGIRAEAEDLAASAGLEVEWAPAEAGFPLGKSAGPVTCLLVAAAPSALQGLALTLTQPWAVVAQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N39</accession>
    <name>Q67N39_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable glycine dehydrogenase (decarboxylating) subunit 2</fullName>
            <ecNumber evidence="4">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glycine cleavage system P-protein subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Glycine decarboxylase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Glycine dehydrogenase (aminomethyl-transferring) subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gcvPB</name>
        <name evidence="5" type="ordered locus">STH1919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-N(6)-lipoyl-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GcvP family. C-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196046.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1919"/>
    <dbReference type="EnsemblBacteria" id="BAD40904">
        <property type="protein sequence ID" value="BAD40904"/>
        <property type="gene ID" value="STH1919"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1919"/>
    <dbReference type="eggNOG" id="COG1003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_5_0_9"/>
    <dbReference type="OMA" id="MHINLHK"/>
    <dbReference type="OrthoDB" id="70707at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00713">
        <property type="entry name" value="GcvPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023012">
        <property type="entry name" value="GcvPB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="PTHR11773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="486" mass="53438" checksum="BC4BF69B0B5BBAE4" modified="2004-10-11" version="1">MALVPLLFEKSTPGKRAVTFPAPDVPTVELSEAIPADLIRQTPAALPELSEVEVVRHFTQLSRLNHGVDVDFYPLGSCTMKYNPKVNEEAARIPGFALTHPLQPEETVQGNLQLLHTLEGWLCEVTGMDRMTFQPAAGAHGELTGILLIRAYHESRGDTERNEVIVPDSAHGTNPATAAMAGYRVVPVKSRPDGSIDVDALRQIVGPKTAALMMTNPSTLGLFDPHVREITEIVHQAGGLCYYDGANLNAIMGYARPGDMGFDVVHLNLHKTFSTPHGGGGPGSGPVGVKSHLVPFLPVPVVEKQGDRYALDWDRPQSIGKIHGFWGNFGVIVRAYAYVLAHGPALKEVSEHAVLNANYVLARLKPYYEPYVDRYCMHECVLSARTLKQETGVRTLDVAKRLLDEGVHPPTIYFPLIVEEALMIEPTETEDKATLDRFIEAMIRIHDEARSNPEYVKSAPHKTPVGRLDEALAARRPNLRYRANPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N52</accession>
    <name>Q67N52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196033.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1906"/>
    <dbReference type="EnsemblBacteria" id="BAD40891">
        <property type="protein sequence ID" value="BAD40891"/>
        <property type="gene ID" value="STH1906"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1906"/>
    <dbReference type="HOGENOM" id="CLU_1271744_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="217" mass="24141" checksum="BECA23637EA4BB89" modified="2004-10-11" version="1">MLLEWRRFEDGQTAVGIQAFVRRSGTFAHDRLYGWGLKHAETTPAHRASFTEDGALLVEWDMGDPARHTRIRRYALNLEQGSVAIVEERFVPEGSDLVYPEAPEAVLHAAQIAAAYRLEDELPRYFASTDVAGAFLEAIPPWPYEVTGVELASVTAFDEYCVPTTEPAQPDADGKAPFVVWLAGQINLIVKVGTVWFETDDAGRTIIRDFRLDEGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N77</accession>
    <name>Q67N77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1881"/>
    <dbReference type="EnsemblBacteria" id="BAD40866">
        <property type="protein sequence ID" value="BAD40866"/>
        <property type="gene ID" value="STH1881"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1881"/>
    <dbReference type="eggNOG" id="COG0803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_0_9"/>
    <dbReference type="OMA" id="LTEQMPK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006129">
        <property type="entry name" value="AdhesinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00691">
        <property type="entry name" value="ADHESINB"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="35258" checksum="7F7AE9A96EA1C45A" modified="2004-10-11" version="1">MAAVTALLLLAGCATADGARRGASGFAEDGRLRVVASIYPIYEFARAVGGDRIELVNLVPPGTEPHDWEPSVGDIRTLNGAQVFLYAGAGFEHWIDKALASLDNRDLIAVETSAGFALLGGNPSHGDESAHDDHDEGLHGDHQEGADEGHHHGGLDPHIWLDPTGAAHIVERIRDGLAAADPGNADVYRTSAAAYLKELEALDREYAAGLAQCERRTFFTTHAAFGYLARRYGLEQRAIMGLAPDAEPTPRALKAVVDAAREQDVRYIFFETLVSDRVARVVADEIGAETLVLNPFEGLTPEQVAAGEDYLSVMRENLANLRIALGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND5</accession>
    <name>Q67ND5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage II sporulation protein M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoIIM</name>
        <name evidence="2" type="ordered locus">STH1823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195951.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1823"/>
    <dbReference type="EnsemblBacteria" id="BAD40808">
        <property type="protein sequence ID" value="BAD40808"/>
        <property type="gene ID" value="STH1823"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1823"/>
    <dbReference type="eggNOG" id="COG1300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085980_0_0_9"/>
    <dbReference type="OMA" id="QDLYYYL"/>
    <dbReference type="OrthoDB" id="2040857at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014196">
        <property type="entry name" value="SpoIIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038973">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02831">
        <property type="entry name" value="spo_II_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="22750" checksum="887379FED7507048" modified="2004-10-11" version="1">MQLYAAWRENLEELLLERGGLVLWHTALFVVGLLFGALALRSLEVETQLELARQISGALQALREGEPPAPGPLLREALFRQARTVALMWVLGVSLVGSVAVMALPLVRGFTSGFAVAYLTAELGARGVLLAAAGHLPQTVLEVPGVILAASASVGFAVEVLTSWRVRRRLSGYYDALARYSNTLLCAAVLLAAAALVEGYVTPHLVRLVLEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF3</accession>
    <name>Q67NF3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative poly A polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1805</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1805"/>
    <dbReference type="EnsemblBacteria" id="BAD40790">
        <property type="protein sequence ID" value="BAD40790"/>
        <property type="gene ID" value="STH1805"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1805"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_5_0_9"/>
    <dbReference type="OMA" id="ILCHTTA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="325"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="388"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="890" mass="96467" checksum="BD2A3FA3304025BB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG1</accession>
    <name>Q67NG1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase (ATP)</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1797"/>
    <dbReference type="EnsemblBacteria" id="BAD40782">
        <property type="protein sequence ID" value="BAD40782"/>
        <property type="gene ID" value="STH1797"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1797"/>
    <dbReference type="eggNOG" id="COG1793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008325_4_0_9"/>
    <dbReference type="OMA" id="AGFRWHK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014146">
        <property type="entry name" value="LigD_ligase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02779">
        <property type="entry name" value="NHEJ_ligase_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_LIGASE_A3" evidence="3">
        <location>
            <begin position="101"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="34280" checksum="67462E43FA0DF714" modified="2004-10-11" version="1">MRLPFPPMLLERAEAPFADDDWVYQVKWDGVRNLTLVEGGRVRHWSRRLRERTALFPEFDGLAAALPGRRAVLDGEIIVLQDGRPSFSAVLERDLAGSTAGAARRRPATLMLFDLLEWDGRELYDVPLAERLALLEAVVPPDEAWQVVSSFPGSDGPALYAAAVARGLEGVVAKRRASRYVPGARSRDWLKVKRRSEMLAVVVGYTNPTGRPGGLLLGAYRDGRLRYIGRVGSGLSGADLAAIRHHLPSGPCPLAEIPRLRERFGGDPGPVVWVEPLLTVRVSFTEWTEEGRLRDPVVVGFSAEPPEAAVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN1</accession>
    <name>Q67NN1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="3 13">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">STH1727</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Adenine glycosylase active on G-A mispairs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="3 13"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1727"/>
    <dbReference type="EnsemblBacteria" id="BAD40712">
        <property type="protein sequence ID" value="BAD40712"/>
        <property type="gene ID" value="STH1727"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1727"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_0_0_9"/>
    <dbReference type="OMA" id="RDPYRVW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="DNA_Glycosylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005760">
        <property type="entry name" value="A/G_AdeGlyc_MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="PTHR42944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01084">
        <property type="entry name" value="mutY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="14">
        <location>
            <begin position="222"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD40712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="365" mass="40652" checksum="265346ED9D7E2257" modified="2004-10-11" version="1">MADRLLDWYSASARDLPWRRTRDPYHIWVSEIMLQQTRVETVIPYYERWMARFPTLEALADAPEEQVLKAWEGLGYYSRARNLHAAAREVVARYGGTVPDDPDAVASLKGIGPYTAGAILSIAFNRPVPAVDGNVLRVIARLYAIVDDIAQLATRRTIEELVRAMIPQDRPGDFNQALMDLGATICTPRRPRCLLCPVRDLCEGRRAGRETELPVKGRAKAPRLVERAAAVIEREGRLLIVRRPQEGLLAGLWELPGGDVPPGVGPEEALQTLLRNALGAEVAVGEHLADVTHVFSHLVWHLRCFRAEVVPGTEVAERADVRWVKVDALGAYPFPAIYHKVFERVLPHTAHGGGGRTRRNAQDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS1</accession>
    <name>Q67NS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DinB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1687"/>
    <dbReference type="EnsemblBacteria" id="BAD40672">
        <property type="protein sequence ID" value="BAD40672"/>
        <property type="gene ID" value="STH1687"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1687"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116635_0_0_9"/>
    <dbReference type="OMA" id="IGGMRPF"/>
    <dbReference type="OrthoDB" id="2047691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007837">
        <property type="entry name" value="DinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05163">
        <property type="entry name" value="DinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="156" mass="18188" checksum="21C1B9EFE3784498" modified="2004-10-11" version="1">MGELLTPASLLEMMEGNRRLTLRVIEAFPEEELFHFKAAEAMRPFSEMVVEFLEIEEAYIRGIVTGEWVLRAPEGLDTKQKLLDACAAVRQRTRELWPQITVERLLTEERDNLFGPQPQTNLSRLIYALENEIHHRGQGYTYLRMLGIQPPPFYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW7</accession>
    <name>Q67NW7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spore germination protein KC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gerKC2</name>
        <name evidence="4" type="ordered locus">STH1641</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKC lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195770.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1641"/>
    <dbReference type="EnsemblBacteria" id="BAD40626">
        <property type="protein sequence ID" value="BAD40626"/>
        <property type="gene ID" value="STH1641"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1641"/>
    <dbReference type="eggNOG" id="ENOG502ZAIE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051140_0_2_9"/>
    <dbReference type="OMA" id="EVECDIN"/>
    <dbReference type="OrthoDB" id="1421701at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008844">
        <property type="entry name" value="Spore_GerAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038501">
        <property type="entry name" value="Spore_GerAC_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35789">
        <property type="entry name" value="PTHR35789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05504">
        <property type="entry name" value="Spore_GerAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02887">
        <property type="entry name" value="spore_ger_x_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="385" mass="42722" checksum="C59A9F6CCB0C8E6E" modified="2004-10-11" version="1">MRRVAALLLLLPLLTGCWGRLELADLALVIALGVDLADNGLYEFTFGIAGPPVSHMKNAPQGDAGIPSRIVVTQRGRSFAGVLREIELRLPRRINLTHTLVVFIGERLAEHGLGDALDFVLRAPEFRLQGLIVMVRGGPVRTLLETEPLMENLQTKALTEIAKAHIGLEVRLWEFFSARATTYRAPLLPVIELMESPDTGAKGQRYAARLGGAAVLRGDRVALYLDAREVRAIKWLRGRGRDGVITVPCGDEPGPESVSFRVVQAGHRVRLHRGGKPAFAVALRGRLRVTEMQCPRPLVEPQVHEQLVQRAEAELRDLMEQVIANLQEAGVDPVFFGEHVRALRPGLWRTVGEERWGETWREVPVTVSVDLRLHTTGLMRNPLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P35</accession>
    <name>Q67P35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1573"/>
    <dbReference type="EnsemblBacteria" id="BAD40558">
        <property type="protein sequence ID" value="BAD40558"/>
        <property type="gene ID" value="STH1573"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1573"/>
    <dbReference type="HOGENOM" id="CLU_2025579_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="122" mass="13267" checksum="68185D71A8650326" modified="2004-10-11" version="1">MVPVGRVFARGSGWLPRTRVPAGPGRAVGRVGPLVIWLVVGGVIGIVLIAVPQPLNVAPGFGLLLGVRMHTSALLEQLLGRVEGRRREPVRPDPDTLLERLARARLAPRRKDRIRRKAQGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P36</accession>
    <name>Q67P36_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1572"/>
    <dbReference type="EnsemblBacteria" id="BAD40557">
        <property type="protein sequence ID" value="BAD40557"/>
        <property type="gene ID" value="STH1572"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1572"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_9_1_9"/>
    <dbReference type="OMA" id="MAGNLWK"/>
    <dbReference type="OrthoDB" id="1938042at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7040" checksum="79D4FAFC07E9A68D" modified="2004-10-11" version="1">MIRASMAGTVWKVLVKPGDAVVPGQDVVILESMKMEIPITAEVGGTVTAVKVGEGDFVNEGDVVVELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P37</accession>
    <name>Q67P37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase, short-chain specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1571</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1571"/>
    <dbReference type="EnsemblBacteria" id="BAD40556">
        <property type="protein sequence ID" value="BAD40556"/>
        <property type="gene ID" value="STH1571"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1571"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_9"/>
    <dbReference type="OMA" id="AMEELFW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="231"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="381" mass="40975" checksum="8D3320982F114982" modified="2004-10-11" version="1">MSLNFDLSPEQEMIRRLAREFAEGEVAPGAAERDKTGRFPVELVRKMGELGLMGIPFPEKYGGTGGDTVSYVLAVEEISRVDGSLGITLAAHCSIGMGPVYSFGTEEQKRRWLPGAARGEYLASFGLTEPGAGSDAAATKTTAVRDGDGWVLNGSKVFITNANYCGYIVCTAVTRPGQGHRGISAFIVPNPTPGFTIGPAYEKMGLRSSDTRPLFFDNVRLPADALLGREGEGFRQFMATLDGGRISIGAMAVGIAQGALDAALAYAKQRVQFGQSISKFQAIQFKLADMAMEIELARNMVLKAAWLKDQGRPFTREAAMAKLFASETAVRAANQAIQIHGGAGYMEDLPVSRFWRDAKLTEIGEGTSEIQRLVIARQLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P60</accession>
    <name>Q67P60_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartokinase</fullName>
            <ecNumber evidence="12">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH1548</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195678.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1548"/>
    <dbReference type="EnsemblBacteria" id="BAD40533">
        <property type="protein sequence ID" value="BAD40533"/>
        <property type="gene ID" value="STH1548"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1548"/>
    <dbReference type="eggNOG" id="COG0527">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_3_2_9"/>
    <dbReference type="OMA" id="LMSCGEV"/>
    <dbReference type="OrthoDB" id="1067792at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="7 12 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="14">
        <location>
            <begin position="3"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="ACT_7" evidence="15">
        <location>
            <begin position="336"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD40533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="42616" checksum="BFD8C6574BEFE0DB" modified="2004-10-11" version="1">MRIIVQKFGGTSVATPEGREAVVRRVRQALDDGYATVVVVSAMGRQGDPYATDTLIQLAQSAGPDLAPREMDLLLSCGEIISSVLMAATLTAAGIPAVALTGGQAGIQTDENYGNAQIVKVDPAPVLSRLKEGLVVVVAGFQGVNAAGEVTTLGRGGSDTTAAALGGALRAEVVEIYTDVDGVKTADPRLVAEARTLSVTTYDEIAQMAHYGAKVVHPRAVEIAMQHRVPIRVRSTFSDNPGTLITYSAEAGVIWSEPFSDRVVTAVTHVVGLAQVVVKTGRDWATPRLFRRLANAGISVDLISVSMESSAFSIPESRAAQAEMILTELGLVDLQVRHGCAKVTVVGSGMRGRPGVMATVAEALYAAEVPIWQTADSHVTISCLIPAADVQRAVQALHDAFELGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK4</accession>
    <name>Q67PK4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Biotin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bioY</name>
        <name evidence="4" type="ordered locus">STH1404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BioY family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195534.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1404"/>
    <dbReference type="EnsemblBacteria" id="BAD40389">
        <property type="protein sequence ID" value="BAD40389"/>
        <property type="gene ID" value="STH1404"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1404"/>
    <dbReference type="eggNOG" id="COG1268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077931_2_0_9"/>
    <dbReference type="OMA" id="AGYLWSY"/>
    <dbReference type="OrthoDB" id="1691691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003784">
        <property type="entry name" value="BioY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34295">
        <property type="entry name" value="PTHR34295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02632">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016661">
        <property type="entry name" value="BioY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR016661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="19090" checksum="92084DE18E6CACD0" modified="2004-10-11" version="1">MHGARLRNLVLAGVMAALLAAVAPVRFFLPGVPGVPVTLQVLVVLLAGGLFRPAWGAAAMGLYLLLGAAGLPVFAGGNAGLQVLVGPTAGYLWSYPLAAAVVGALALPAGHGADASVRPGRVRMFGAMLAGLAVIYVCGAGWAWLIGGQGLAAVLAGWVLPFVPFDLVKAAVAAEGAAALRRALARAGLLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL2</accession>
    <name>Q67PL2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1396"/>
    <dbReference type="EnsemblBacteria" id="BAD40381">
        <property type="protein sequence ID" value="BAD40381"/>
        <property type="gene ID" value="STH1396"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1396"/>
    <dbReference type="HOGENOM" id="CLU_2412089_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="92" mass="9733" checksum="FF0C2A42926B21E4" modified="2004-10-11" version="1">MFGFAPVWPAAAPLFAAPVINVWWYELKNVSSGGVVSTGVLDLGFLTSNSKGNSLTDQGGDATYSATWYGFLSDTDWVDTPYANMAGFQVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL9</accession>
    <name>Q67PL9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1389"/>
    <dbReference type="EnsemblBacteria" id="BAD40374">
        <property type="protein sequence ID" value="BAD40374"/>
        <property type="gene ID" value="STH1389"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1389"/>
    <dbReference type="eggNOG" id="COG0425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_0_3_9"/>
    <dbReference type="OMA" id="SWTITVQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9790" checksum="7BC3D8964FE7FDA7" modified="2004-10-11" version="1">MLGEACPYPLIMAKQKINEVAPGGRLIIDFDCTQATETLPRWATESGHAVEEFRKTGPAQWRIVIRKQGDGSERVTPDDYTCSVPGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ9</accession>
    <name>Q67PQ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nusB</name>
        <name evidence="15" type="ordered locus">STH1349</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(967) in 16S rRNA + S-adenosyl-L-methionine = 5-methylcytidine(967) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42748"/>
            <dbReference type="Rhea" id="RHEA-COMP:10219"/>
            <dbReference type="Rhea" id="RHEA-COMP:10220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.176"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the NusB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195479.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1349"/>
    <dbReference type="EnsemblBacteria" id="BAD40334">
        <property type="protein sequence ID" value="BAD40334"/>
        <property type="gene ID" value="STH1349"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1349"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_9"/>
    <dbReference type="OMA" id="RVNRQHH"/>
    <dbReference type="OrthoDB" id="1064993at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004573">
        <property type="entry name" value="rRNA_ssu_MeTfrase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00563">
        <property type="entry name" value="rsmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="10 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="14">
        <location>
            <begin position="179"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="13">
        <location>
            <begin position="272"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD40334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="459" mass="50413" checksum="802A844AA326F405" modified="2004-10-11" version="1">MPRPKSGPPRTGREAALRALYDVDIKAAYANLALDHHLSRSQLDGRERALATELAYGVTRRRATLDWAIGQVAARPLEKMDPWVRNILREAAYQILYMDRIPPAAAVDQAVELARQYGHEGVAKFVNGVLRNLIRRLPGLPWPDEAADPVRALAVQHSYPEWLVAQWLERFGRGEAVRLLEAGNQVPPLTVRVNRLKATREEAAAALAAEGVRTEPTRHSPQGLIIQDLTSASSLERLEAMKQGLITVQDESSMLAAAVLNPQPGWMVIDVAAAPGGKATHLAELMDNRGRVIALDIHPHKVALIEENAERLGTTIVEAVCLDARRVGEAMPERADAVLCDLPCSGLGTLARRPDARWRKSPEDVEALVPIQRAILESAAKALKPGGVLVYSTCTIQPKENEELVEGFVADHPEFRFDRVWDYLPGTISPEGQAEGYVQLLPHVHGTDGFFIARMVKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS8</accession>
    <name>Q67PS8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">D-alanine aminotransferase</fullName>
            <ecNumber evidence="5 10">2.6.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1330</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha-keto acid in the first half-reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2-oxoglutarate + D-alanine = D-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:15869"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="EC" id="2.6.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.21" evidence="5 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1330"/>
    <dbReference type="EnsemblBacteria" id="BAD40315">
        <property type="protein sequence ID" value="BAD40315"/>
        <property type="gene ID" value="STH1330"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1330"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_4_1_9"/>
    <dbReference type="OMA" id="RGFQFAD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047810">
        <property type="term" value="F:D-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046416">
        <property type="term" value="P:D-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005784">
        <property type="entry name" value="D_amino_transT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01121">
        <property type="entry name" value="D_amino_aminoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="30914" checksum="03E63F22DB812BA4" modified="2004-10-11" version="1">MYLNGQYVPYEEAKIPVEDRAFLFADGIYEVVRVYGGRPFDLEPHLRRLARSARELRLPEPDLEGLTRVTHELIRRNGVDEGTVYYQISRGVHAPRAHAFPGQPVEPTVLVLTHPLTPEAVAAGERRRQEGVTAITVPEQRWARCDIKSVSLLPNVLAKQQAAEQGAYEALFVRDGFVIEGSSSNVFAVIDGAIVTYPACNYILNGIARQRVIRDARSLGYTVLEQGIPLAALDRCQELFVTSTTSEVTPVVAVDGRPIGDGKVGPVVRALQQAYRAAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU8</accession>
    <name>Q67PU8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1310"/>
    <dbReference type="REBASE" id="10267">
        <property type="entry name" value="M.StpORF1310P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40295">
        <property type="protein sequence ID" value="BAD40295"/>
        <property type="gene ID" value="STH1310"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1310"/>
    <dbReference type="eggNOG" id="COG0270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006958_2_4_9"/>
    <dbReference type="OMA" id="FPDNYFF"/>
    <dbReference type="OrthoDB" id="1178320at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="7">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="486" mass="54889" checksum="69D5721356618E9A" modified="2004-10-11" version="1">MKKLERLASGGRPRVLDLFAGCGGLSLGFQRAGFEILAAVEMDPHAARSHAINFHPGDRFDLHAKPRDISQEQPDQVLGELYPGERAEDLVDIIIGGPPCQAYARVGRAKLREIWRHPEGYKLDPRGDLFLHYLYYVDRLKPVALVMENVPDALNYGGHNIAQEVADWLEDRNYVCRYTLLNAVHYGVPQMRERMFLIAVAGELGVEPEFPVPTHAFTLPAGYKNARKVAMRTLGLLQPERLGSFVSPPTVDPALPPAVTAYEAIGDLPPITAHLENRIGRGPRRFTEQVPYAGPPHSDYARLMRTWPGFEALHGISDHVIRYLPRDYRIFARMRPGDQYPEAYAHALSLFMAAVERLRREGVDIAPGSEPYQRLWKEYVPPYDVSKFPNRWWKMERDLPVRTLMAHLGKDSYTHIHYDDAQARTISVREAARLQSFPDGFRFAGTMNPAFRQIGNAVPPLLAWRVGAAVREAIETALGVRLPDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY4</accession>
    <name>Q67PY4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative sphingosine-1-phosphate lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="journal article" date="2010" name="Structure" volume="18" first="1054" last="1065">
            <title>Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism.</title>
            <authorList>
                <person name="Bourquin F."/>
                <person name="Riezman H."/>
                <person name="Capitani G."/>
                <person name="Grutter M.G."/>
            </authorList>
            <dbReference type="PubMed" id="20696404"/>
            <dbReference type="DOI" id="10.1016/j.str.2010.05.011"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 2-507</scope>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="journal article" date="2016" name="Bioorg. Med. Chem. Lett." volume="26" first="2293" last="2296">
            <title>Creation of a S1P Lyase bacterial surrogate for structure-based drug design.</title>
            <authorList>
                <person name="Argiriadi M.A."/>
                <person name="Banach D."/>
                <person name="Radziejewska E."/>
                <person name="Marchie S."/>
                <person name="DiMauro J."/>
                <person name="Dinges J."/>
                <person name="Dominguez E."/>
                <person name="Hutchins C."/>
                <person name="Judge R.A."/>
                <person name="Queeney K."/>
                <person name="Wallace G."/>
                <person name="Harris C.M."/>
            </authorList>
            <dbReference type="PubMed" id="27013389"/>
            <dbReference type="DOI" id="10.1016/j.bmcl.2016.02.084"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS) OF 2-507</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15869777">
            <id>Q67PY4</id>
        </interactant>
        <interactant intactId="EBI-15869777">
            <id>Q67PY4</id>
            <label>STH1274</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3MAD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=2-507"/>
    </dbReference>
    <dbReference type="PDB" id="3MAF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.97 A"/>
        <property type="chains" value="A/B=2-507"/>
    </dbReference>
    <dbReference type="PDB" id="3MAU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B/C/D=2-507"/>
    </dbReference>
    <dbReference type="PDB" id="3MBB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B=2-507"/>
    </dbReference>
    <dbReference type="PDB" id="5EUD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.24 A"/>
        <property type="chains" value="A/B=2-507"/>
    </dbReference>
    <dbReference type="PDB" id="5EUE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.83 A"/>
        <property type="chains" value="A/B=2-507"/>
    </dbReference>
    <dbReference type="PDBsum" id="3MAD"/>
    <dbReference type="PDBsum" id="3MAF"/>
    <dbReference type="PDBsum" id="3MAU"/>
    <dbReference type="PDBsum" id="3MBB"/>
    <dbReference type="PDBsum" id="5EUD"/>
    <dbReference type="PDBsum" id="5EUE"/>
    <dbReference type="SMR" id="Q67PY4"/>
    <dbReference type="DIP" id="DIP-59009N"/>
    <dbReference type="STRING" id="292459.STH1274"/>
    <dbReference type="EnsemblBacteria" id="BAD40259">
        <property type="protein sequence ID" value="BAD40259"/>
        <property type="gene ID" value="STH1274"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1274"/>
    <dbReference type="eggNOG" id="COG0076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028929_1_0_9"/>
    <dbReference type="OMA" id="TMAPHSE"/>
    <dbReference type="BRENDA" id="4.1.2.27">
        <property type="organism ID" value="6177"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q67PY4"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8 9 10" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2 4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3MAD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="3MAF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="3MAU"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="5EUD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="5EUE"/>
        </source>
    </evidence>
    <sequence length="507" mass="54645" checksum="AE4EF2F847858910" modified="2004-10-11" version="1">MLSAFSPPLPCDPARSHPTPEFPSSLQDYCEIRGIQSQPPARRDPTMDWLASLRSQIKPYRDRFPSHARLPRAGLPRAEILAEIAAMGAAESPAWRDGYASGAVYHGDEHHIAFLNEVYALQSQSNPLHPDLWPSTAKFEAEVVAMTAHMLGGDAAGGTVCGTVTSGGTESLLLAMKTYRDWARATKGITAPEAVVPVSAHAAFDKAAQYFGIKLVRTPLDADYRADVAAMREAITPNTVVVAGSAPGYPHGVVDPIPEIAALAAEHGIGCHVDACLGGFILPWAERLGYPVPPFDFRLEGVTSVSADTHKYGYGAKGTSVILYRRPDLLHYQYFIAADWPGGLYFSPTFAGSRPGALSATAWAAMLSLGEEGYLDATRRILQAADRLKAGVRAIPSLKILGDPLWVIAVASDELNIYQVMEEMAGRGWRLNGLHRPPAFHVALTLRHTEPGVVDRFLADLQDAVAQVRAHPEKATGMAPVYGMAAAAPPELVRQVLTGFIDLLYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ7</accession>
    <name>Q67PZ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ordered locus">STH1261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1261"/>
    <dbReference type="EnsemblBacteria" id="BAD40246">
        <property type="protein sequence ID" value="BAD40246"/>
        <property type="gene ID" value="STH1261"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1261"/>
    <dbReference type="eggNOG" id="COG0461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_3_0_9"/>
    <dbReference type="OMA" id="YFQCAKV"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006273">
        <property type="entry name" value="Orotate_PRibTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01367">
        <property type="entry name" value="pyrE_Therm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="41"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Orotate binding" evidence="4">
        <location>
            <begin position="27"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="116"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="20671" checksum="AA6014314D1BF809" modified="2004-10-11" version="1">MLDILRATGALLEGHFQLTSGRHSDRFFLMPHTLQHPLQTERLCRALAARFQEDEVETVVGPATGGIILAYEVARQLERLTGRPVRAIFTEKTEDGGMALKRQWRLRSGERVLVVEDAITTGGSVVKAIAALQAYQPALVGVGCIVDRSRGAVDFGVPLRPLIRVDVESWPPDRCPLCLAGVPVVKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q81</accession>
    <name>Q67Q81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1177</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195308.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1177"/>
    <dbReference type="EnsemblBacteria" id="BAD40162">
        <property type="protein sequence ID" value="BAD40162"/>
        <property type="gene ID" value="STH1177"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1177"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1209289_0_0_9"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25535" checksum="6DD6E107B71FE7AE" modified="2004-10-11" version="1">MGIQEAEKTSVAVYSLERIWGAHLSQRELEVGRLLVSGRSTQEIAAALFISENTVKTHLRNLYRKTRTRSRVELYRALMEAASAPARIGTVAEDLPAEFQRIFADLHQDQAEGMNATVVLLHLEFFQSGLATRVPGTILTDLLATSIRSRDHLLPWRDDLYLLLLPGSDRTAAVEVADRLVRKVQRWAQRKRLMMRAAVGAAAIAEGAGTAPSVVQLAVQRLRHVDYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q84</accession>
    <name>Q67Q84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195305.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40159">
        <property type="protein sequence ID" value="BAD40159"/>
        <property type="gene ID" value="STH1174"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1174"/>
    <dbReference type="HOGENOM" id="CLU_1229358_0_0_9"/>
    <dbReference type="OrthoDB" id="2016666at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023833">
        <property type="entry name" value="Signal_peptide_camelysin"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04088">
        <property type="entry name" value="cognate_SipW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="24602" checksum="3377FDE0BAB2BED1" modified="2004-10-11" version="1">MGLRFVWSSLLSVILTGGLLGATTLALFTDQAESSGQFTACTLPLRCSTVVNTESALFGGSAPARITKAGPTEHVLQPGEQHDITITVELPKEAGNAYQNAQGTLTITFFAPQRTNMEWVPLGPKLKADNVAVPATGRVYDLCQVELTGGEVVNLDKARGIWIQVWDPTPKWNWAPIIGDDHNLWSDNDLPSGGYNVYIFMEDGTVYRTLTIPHTRNQTNERRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC6</accession>
    <name>Q67QC6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IstB helper protein C-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1132"/>
    <dbReference type="EnsemblBacteria" id="BAD40117">
        <property type="protein sequence ID" value="BAD40117"/>
        <property type="gene ID" value="STH1132"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1132"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_8_2_9"/>
    <dbReference type="OMA" id="FEPMEAQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="103" mass="11836" checksum="067310E10B191319" modified="2004-10-11" version="1">MYLSPRLLLIDEMGYIPFEPMEAQVFFQLISKRYDRGSPIIVTSNKSYGDWGSIFGDPIIAAVILDRLLHRSVTVNIRGESYRLREKRRAGILPPKREVTPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF7</accession>
    <name>Q67QF7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1101"/>
    <dbReference type="EnsemblBacteria" id="BAD40086">
        <property type="protein sequence ID" value="BAD40086"/>
        <property type="gene ID" value="STH1101"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1101"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_3_9"/>
    <dbReference type="OMA" id="KLPLIWH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="10"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="181"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="36645" checksum="3F71F4D14D243DDE" modified="2004-10-11" version="1">MLHVCSDTNIGGAGRYVLTLLTQPRIAERFTVAAACPEGALAAALRRAGVTVFPYAGADRSLSWSGLRELVGLIRRWRPHVVHTHGALAGRIAGRLGGARVVYTKHGLAAAVEASVQVRSPGAWLRRLAVRRFADRIVAVSGAVRDALIAQGADPAAVRVIPGGVDLGPYEQAPPPVAGVVGALGRLEREKGFDVLLEAMARLRGEARLLLGGDGSQRQALAARVEAEGLPVELVGFVDDVPAFLGRTGVFVVPSRSEGLGLVAVEAMAAGRPVVASRTGGLPEVVVDGETGLLVAPEDPDGLARAIRMLLADPERSARMGAAGRERARALFSAERMAAETAALYEELIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK3</accession>
    <name>Q67QK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195187.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1055"/>
    <dbReference type="EnsemblBacteria" id="BAD40040">
        <property type="protein sequence ID" value="BAD40040"/>
        <property type="gene ID" value="STH1055"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1055"/>
    <dbReference type="HOGENOM" id="CLU_085018_0_0_9"/>
    <dbReference type="OrthoDB" id="1845499at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="23627" checksum="0B548C7EF0DCA4CF" modified="2004-10-11" version="1">MTETELRELTAGFVPWNQWVRPVAESKVALVATAGVYLKHGLQEPYDDGRPGGDPSFREFPVVVRYEDLAVAGPLPAPAREDLNLVFPLERLRAMAESRAIDAVAPFAYSFSGTITDPVPLLAGYGPSVAYRIRRMGADVALVCAAGGTGRLTAALVARLIELAGVPTVVLDGEADGLRGLGIPRGVAIRRPAAPGDAEGQQRLLRAALEAAWGLHEPGVVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM3</accession>
    <name>Q67QM3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4352 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195167.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1035"/>
    <dbReference type="EnsemblBacteria" id="BAD40020">
        <property type="protein sequence ID" value="BAD40020"/>
        <property type="gene ID" value="STH1035"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1035"/>
    <dbReference type="HOGENOM" id="CLU_1277085_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029051">
        <property type="entry name" value="DUF4352"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029050">
        <property type="entry name" value="Immunoprotect_excell_Ig-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11611">
        <property type="entry name" value="DUF4352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269318" description="DUF4352 domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4352" evidence="4">
        <location>
            <begin position="95"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="21872" checksum="2F98DF1E588390F1" modified="2004-10-11" version="1">MRRRKILIWAAAALLAVLVAGCGKYADQTGPNRTDPSPSSSAGSAGGNQPATGGGGDGGGDAVGNAAGGAAGDGAGPGGGGGAEPAGKVQLGPLAVGESAQVGPLTVTMHRAETVDQAPAPGYTYLLIEVTVENGGSAPYTVNPTEQHKVQTPEEKNAPYNLQATALRTPKFQGTLRQGESGSGWLGFLAKKLEGTYTYTFTHPEYGEATWEFTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN7</accession>
    <name>Q67QN7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SGNH_hydro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195153.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1021"/>
    <dbReference type="EnsemblBacteria" id="BAD40006">
        <property type="protein sequence ID" value="BAD40006"/>
        <property type="gene ID" value="STH1021"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1021"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051180_1_0_9"/>
    <dbReference type="OrthoDB" id="1247435at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269623" description="SGNH_hydro domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="2">
        <location>
            <begin position="65"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="25696" checksum="769DD35E9D7C7616" modified="2004-10-11" version="1">MRRVLVVALAVAAIAAVALWRAGRPSPKQQSALQAPAVELARAALPEVLTPDPRVDRSGWPVIVAFGDSLTAGLGVDPDRNYPSQLQALLDERGYRYRVVNAGVSGELTAGGLSRVDQVLEHRPAVVILELGANDGMQARPVAEVRENLAGIIARLQEEGVAVLLAGMRAPPNHGPEYEAAFAQVYPDLAAEFGVPLIPFFLEGVAGSPYLNQRDGIHPTAQGYAIVVRTVLSALEPLLQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR6</accession>
    <name>Q67QR6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycolate oxidase iron-sulfur subunit</fullName>
            <ecNumber evidence="4">1.1.99.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH992</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of a complex that catalyzes the oxidation of glycolate to glyoxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-lactate + A = AH2 + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:15089"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>A + glycolate = AH2 + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:21264"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="1.1.99.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.14" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195124.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH992"/>
    <dbReference type="EnsemblBacteria" id="BAD39977">
        <property type="protein sequence ID" value="BAD39977"/>
        <property type="gene ID" value="STH992"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH992"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_0_1_9"/>
    <dbReference type="OMA" id="VYQDACH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012257">
        <property type="entry name" value="Glc_ox_4Fe-4S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479:SF17">
        <property type="entry name" value="PTHR32479:SF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000139">
        <property type="entry name" value="Glc_ox_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="428" mass="46246" checksum="0B5D44B19B35C680" modified="2004-10-11" version="1">MKVQISEDMILNCMRCGFCLPACPTYRYKQVESASPRGRIALMRAVMEGKLDVLDIAGPLDTCLGCRACEPACPAGVTYGAILEQGRAQLNQVRPLPGVVRWAYRWLLGTPGGIRFSAWALWLYQKLRLNALAHRLNLVEKIGGKGLADMERAIPPVASPARRAARKPAYPAAGNRRYRVAFFMGCLSDIVFFETNQNAIEVLTGLGCEVVLPAGQGCCGAVHSHAGEHELAVAQAKRNIAAFETSGCDFIVSTAGGCGAALREYDKLLADDPEWADRARAFSSRCRDFSELVEQLGPIPMGDMTGTYTYQDSCHLRNVQKVAAPPRNLLKSMPGARFVELPEADRCCGAAGTYMITQALLSDRILDDKAQKVAQTGAATLVVANTPCHLEMLEGIQRAGLADRVQVRHIADVVAEAMRKAPAAGGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU2</accession>
    <name>Q67QU2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH966"/>
    <dbReference type="EnsemblBacteria" id="BAD39951">
        <property type="protein sequence ID" value="BAD39951"/>
        <property type="gene ID" value="STH966"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH966"/>
    <dbReference type="HOGENOM" id="CLU_1642870_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="17938" checksum="552BD5ED85FFAB86" modified="2004-10-11" version="1">MLSTPGRTRPAGTDRGRPEPESGRESEHVRFRPDQRPSKWGATAAVVDRQRAEDVVACLSALQGTPFDAVVRLSDLTEIKGYRYVLLQVSLDFNHPHFPRIADTLDELGGSEFQLIVVETPEGAVYLIVDKHNLPDEEMRPDHPLLRRLADWLGRAMASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY5</accession>
    <name>Q67QY5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH923</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195055.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH923"/>
    <dbReference type="EnsemblBacteria" id="BAD39908">
        <property type="protein sequence ID" value="BAD39908"/>
        <property type="gene ID" value="STH923"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH923"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_9"/>
    <dbReference type="OMA" id="DHQVVRR"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="5">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="218" mass="23933" checksum="32056618FAB142EE" modified="2004-10-11" version="1">MKVLIVDDHLMLRKGVLSVLSNTDLEVIGEASNGQEALELVPKLKPDLVLMDINMPVLDGVEATRKLKQMYPDLKVVILTVSEIDKDLFEAIKAGADGYLLKNLGPEELVSSLRAAISGEAPISSVMAAKMLKEFRQPRANTTGKQPGQQLSPREIEVLRLASTGLTYKEIAAKLYVAESTVKNHMRHILEKLHLRNRSEAVGYAIRTGLAGEPPSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY7</accession>
    <name>Q67QY7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195053.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH921"/>
    <dbReference type="EnsemblBacteria" id="BAD39906">
        <property type="protein sequence ID" value="BAD39906"/>
        <property type="gene ID" value="STH921"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH921"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_9"/>
    <dbReference type="OMA" id="QHENKEY"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="158"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="25201" checksum="909E4FBA1B8183D4" modified="2004-10-11" version="1">MSPIRVLLVDDHAILREGIRYLLSASGEVEVIGEAQDGVEALEMVEKLRPDAVLMDIAMPRMNGIEATKELKKRHPDLPVLILSMYDSEEYVLPILRAGAAGYVLKRAAAQELVSALKAVTSGQVILHPDVARTVMDNLQAQESPQGPPVAPRGASEAQAQLAQLTEREREVLTLIAHGLTNQQIAERLFISIKTVQAHRANLMEKLDLHDAVELTKFAIKSGLLPLDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R43</accession>
    <name>Q67R43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH865"/>
    <dbReference type="EnsemblBacteria" id="BAD39850">
        <property type="protein sequence ID" value="BAD39850"/>
        <property type="gene ID" value="STH865"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH865"/>
    <dbReference type="eggNOG" id="ENOG5032T03">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117571_0_0_9"/>
    <dbReference type="OMA" id="RYPEEIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014099">
        <property type="entry name" value="Spore_coat_GerQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09671">
        <property type="entry name" value="Spore_GerQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038931">
        <property type="entry name" value="GerQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02728">
        <property type="entry name" value="spore_gerQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="19755" checksum="A64A878F56D91AB1" modified="2004-10-11" version="1">MSKPMMPYGRPGWPVMPQMTPGMMPGMMPGMMPGMMPGMMPGMMPGITPGMVPGMTPGTTPGMMPPMTSPQMMMPPGAPPELVYGGQVMIPQEQSYVENILRMNRGKTATVYATYDNNPEWAARVFRGQVENAARDHVVLSDPQTGMRYVILMVNIDYLTFDEPLRYPEEIYRPPGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R69</accession>
    <name>Q67R69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH839"/>
    <dbReference type="EnsemblBacteria" id="BAD39824">
        <property type="protein sequence ID" value="BAD39824"/>
        <property type="gene ID" value="STH839"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH839"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_3_2_9"/>
    <dbReference type="OMA" id="WHGHYVK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008272">
        <property type="entry name" value="HB-CoA_thioesterase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006684">
        <property type="entry name" value="YbgC/YbaW"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003230">
        <property type="entry name" value="YbgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00051">
        <property type="entry name" value="TIGR00051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01328">
        <property type="entry name" value="4HBCOA_THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15977" checksum="AC07407FDA1BA840" modified="2004-10-11" version="1">MVRTQVRVRYADTDAMGVVYHANYLIWFEVGRNELMRAWGAPYADFERRGILVPVTEAQVRWVHPARYDDVLEVQTRMERLTPARVTFAYRIVRAEDGRLCCEGTTTHGFLGPGGRPAALPKLAPDLWRLFNERLQAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R78</accession>
    <name>Q67R78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194963.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH830"/>
    <dbReference type="EnsemblBacteria" id="BAD39815">
        <property type="protein sequence ID" value="BAD39815"/>
        <property type="gene ID" value="STH830"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH830"/>
    <dbReference type="eggNOG" id="COG1988">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067817_2_0_9"/>
    <dbReference type="OrthoDB" id="903022at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="283" mass="30910" checksum="EE6B8AD5B3B36369" modified="2004-10-11" version="1">MDSLTHVLMGHAMGALATAVSPEAGPAVYWAALLGNSLPDVDVPLSFLLRRGIQLHRTYTHTLVGAAALSAASAGLLAAAFPSAGAGPLFFWTLLGSLAHLAVDGLNLFGVRPFWPVSRRIVELGVLHITDPWLLLLLGLPAVGAKAGWLSARWVGAAFLAMWLYVAQRHRTARRLLHELLQAGSLRARVVPWFSGWRYVAETAHSVEFGRWAGGRRVALETFARQDDPRIAATLADPRVSQFLREAEYPVALVQEGEVVWIDALRRLRADFRPLRVPVRAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC6</accession>
    <name>Q67RC6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein, glutamate-rich</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194915.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH782"/>
    <dbReference type="EnsemblBacteria" id="BAD39767">
        <property type="protein sequence ID" value="BAD39767"/>
        <property type="gene ID" value="STH782"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH782"/>
    <dbReference type="HOGENOM" id="CLU_990195_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="30225" checksum="A7DE50408A6FC124" modified="2004-10-11" version="1">MSSIRLQEIMTRAVVGRCDRRVVWTHTAPADGADCVLGVHVGNAQLTVEPGPSGPEVRLTAVCEVWCGAGDGTRVERITCTHTEPADVPLVARVVGETETTGRLLRGARCLEAEVREGLFHLTIEMHIALEAVGTARLWVKAFDLEEEGAADLDAFGSGTSDSSQSVEEPVADWEGQTEAETWAEPETWAEPEEWTGTAPDGEPEEGTERAGKAEGQADPEPEPLAEPEPPSQADGEPVAWDAFRTDMAAVASVQDRESRRTSTAVTRFRGPTSARITIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE2</accession>
    <name>Q67RE2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194899.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH766"/>
    <dbReference type="EnsemblBacteria" id="BAD39751">
        <property type="protein sequence ID" value="BAD39751"/>
        <property type="gene ID" value="STH766"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH766"/>
    <dbReference type="eggNOG" id="ENOG5031MDW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106324_0_0_9"/>
    <dbReference type="OMA" id="CEVITIP"/>
    <dbReference type="OrthoDB" id="1314147at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="19510" checksum="D15D90D8C42FBCF3" modified="2004-10-11" version="1">MQLVRIGDKVINPERIYRMVDRMLELRSQGLSQQEVAEILGVDRTLISRLESVGEMRKGKRIALVGFPVANGPELTAMAAAEGVDFILLMSDQERHEFARSQNGVELLNQVMRLIARARACDAVIFIGSDERLKMVEALVGPHVIGIEIGRSPMKEDVYVSPEKIRNLIRSLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF9</accession>
    <name>Q67RF9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate regulon sensor protein PhoR</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH749</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Member of the two-component regulatory system PhoR/PhoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoB in response to environmental signals.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH749"/>
    <dbReference type="EnsemblBacteria" id="BAD39734">
        <property type="protein sequence ID" value="BAD39734"/>
        <property type="gene ID" value="STH749"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH749"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_9"/>
    <dbReference type="OMA" id="TRFWRGS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014310">
        <property type="entry name" value="Sig_transdc_His_kinase_PhoR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02966">
        <property type="entry name" value="phoR_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="101"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="223"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="444" mass="48498" checksum="60C9E2CD3A13A2A6" modified="2004-10-11" version="1">MAQMRRPYRPALLWFALPPAAGVTVALAYAASGRLEAALARGPRALDLAVWLALLAALLIPPVYGYREASGLARTAERRADAAHREARRAAQERLDRLLAEKAQMDLILQHMADGILLLDERRRIVLVNPAAERMLGVSRHEALGRDHLEVTHHFELDERLARVLATGRPEALEIRRAHPQEQILEARLALAPSGGERGVLVMLRDITRSRQLEQMRTEFVANVTHELRTPLTSIQGFAETLLEGALDEPETARHFVEIMLRESRHLGALIDELLDLSRVESGKFRMQRRPTVPAELIAATAARFAQKAERAGVQLVTEAPDGLPLINGDPDRLVQVLSNLVENAIKYTPAGGRITLSARRDGDGVRIAVADTGAGIPQADLGRIFERFYRVDKARSRATGGTGLGLAIAKHIVEAHGGTIGVESEVGKGSTFTVILPALTGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ8</accession>
    <name>Q67RJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dmsD2</name>
        <name evidence="1" type="ordered locus">STH710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH710"/>
    <dbReference type="EnsemblBacteria" id="BAD39695">
        <property type="protein sequence ID" value="BAD39695"/>
        <property type="gene ID" value="STH710"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH710"/>
    <dbReference type="eggNOG" id="COG3381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077650_0_0_9"/>
    <dbReference type="OMA" id="PEENDYV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="SSF89155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="227" mass="25380" checksum="354B2F739AEF23C4" modified="2004-10-11" version="1">MQGELETVLEARGFAYALLMQAFLVEPQREQIENLAETGAVRLFPFGQDHPAIQQGTAAVGAFLADPANRSAEAVDRLVWDYTRLFVGPARLPAPPYESAYRTVDRLVFQEVTLEVREAYRRYGLVAPKLGTEPDDHIGLELSFLYETCRLAAGAAAAGDIDGVHTILQDQQAFLDDHLMQWVPRFAADVRQHAETAYYRGWADLLAGFAETDRDLLEELLAEQPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK6</accession>
    <name>Q67RK6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative coenzyme A ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194835.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH702"/>
    <dbReference type="EnsemblBacteria" id="BAD39687">
        <property type="protein sequence ID" value="BAD39687"/>
        <property type="gene ID" value="STH702"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH702"/>
    <dbReference type="eggNOG" id="COG1541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_2_9"/>
    <dbReference type="OMA" id="GVAPHFQ"/>
    <dbReference type="OrthoDB" id="839891at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding_C_2" evidence="1">
        <location>
            <begin position="347"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="445" mass="49378" checksum="AACA59B60622F03A" modified="2004-10-11" version="1">MQIELERSHWQPEAERMGRAELARLQAERLRAQVERALASPFYRRLWQGVGPIDPADFRTPADVARLPFLHKSDLVDAQLRKPPLGDLPMSPAEERREVYPVHVVGGSLYTVYGERDLARTAEIGARIMWGLGLRPGELIHNAFGYGLFAGGISWHRAARAMGAAIIPIGTDSVKRQVEMLFNFRPVMLIGAPSHAVYLAERIQERGLSPRDIGLRYGLFGGEPGAGEPITRKRLERLYGCPAFDFYGITEVGPLLAGECRLQQGLHWAEDHVLVEVINPATMAPCAEGETGVLVLTDLTREDMPLIRYWTGDMATLVTEPCGCGRTLARSPGGVRGRLDELVMIRGAKFYPVQVERVLSSYGTLADEYRIVLERDPATWLDRCTVQVEAAPGEFPSPALEERISRRLSDELAVDVAVQILPYGSLERSPRRVRIVDRRAGSGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL0</accession>
    <name>Q67RL0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YceI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH698</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0312 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194831.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH698"/>
    <dbReference type="EnsemblBacteria" id="BAD39683">
        <property type="protein sequence ID" value="BAD39683"/>
        <property type="gene ID" value="STH698"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH698"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1255414_0_0_9"/>
    <dbReference type="OMA" id="EFITFTP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269510" description="YceI domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="YceI" evidence="4">
        <location>
            <begin position="49"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="220" mass="22865" checksum="B69A20B9C27976DB" modified="2004-10-11" version="1">MSLALVALLAAGCARGAAQPPVEPQPAAETGTAPAAAEDSTSAGVDGTAYYVDTAQSQASYAVDETFLGQRLDVTAVGRTSALSGAIVLQDGVIQPSTVEVDLTTLRSDQSRRDNQVQRVLDTRNHPKAVFRIQGAEGDPVLTEGQEVPIRLTGTMTIKGVDRPLTFEGTAKLTGGVLTLTAQTTFAMTDFGVDPPNIANFVAVEDEVTVSVTFVGQAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL2</accession>
    <name>Q67RL2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH696"/>
    <dbReference type="EnsemblBacteria" id="BAD39681">
        <property type="protein sequence ID" value="BAD39681"/>
        <property type="gene ID" value="STH696"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH696"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1199303_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="25169" checksum="CAA193B06F9744ED" modified="2004-10-11" version="1">MVALAQAQLQDVIRNPLLVLFAALLPAMGIMMRLVMPESARPLLVVMLSVMAPYFVGWNVAGITLAEEKEKRRLEALFLTPVRPWQVVMVKGALALAGTLVMGAVMLAVGGKVPVRPGIWFLAYLLLTLFAVGAGTLLGLLFKDMRSMSIGGTPVQILLLFATTLPWALAGPVLWEVQAWLPTRPVVDLMQAGFFGEEVPVLRHALVMLGYVAAVWLANVSLVRRLSFLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU2</accession>
    <name>Q67RU2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4-aminobutyrate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH616</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194749.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH616"/>
    <dbReference type="EnsemblBacteria" id="BAD39601">
        <property type="protein sequence ID" value="BAD39601"/>
        <property type="gene ID" value="STH616"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH616"/>
    <dbReference type="eggNOG" id="COG0160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_0_9"/>
    <dbReference type="OMA" id="TSGQMSC"/>
    <dbReference type="OrthoDB" id="1322538at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003867">
        <property type="term" value="F:4-aminobutyrate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009448">
        <property type="term" value="P:gamma-aminobutyric acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004632">
        <property type="entry name" value="4NH2But_aminotransferase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00700">
        <property type="entry name" value="GABAtrnsam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="457" mass="49441" checksum="BB5EE75AD7914CE5" modified="2004-10-11" version="1">MTQTTRYVRLVTEVPGPRSRELMARKERVVANALSIHVPVAIQEARGALVTDVDGNVFIDLAGGMGCMNVGHSHPRVVEAIQRSAAQFTHTDFSVIMYESYIRLAERLAALAPGDFPKKACFFNSGAEAVENAIKIARKYTGRRAIIALEGAFHGRTNLAMALTSKVKPYKEGFGPFAPEIYRVPTPYTYRRPAGMSEAEYVRFCADALERALITHVSPDEVAAIILEPVQGEGGFIPLHPDYLARVSQLARKHGFLIIADEIQSGFGRTGTFFASEQLGLVPDLICVGKSLAAGMPLSGVIGRAEVMDAPEDSTIGGTYVGNPVACDAAHAVLDIMEEEGLVSRARAIGDLMRRRFQELAVQLESIPGSRLQIGEIRGLGAMLGVELVTDRATRAPATAEAAEVVKRAWQRGVVVVKCGIYGNTLRMLLPLVITDDQLNEALDIIGQICTEIARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S94</accession>
    <name>Q67S94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194598.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH464"/>
    <dbReference type="EnsemblBacteria" id="BAD39449">
        <property type="protein sequence ID" value="BAD39449"/>
        <property type="gene ID" value="STH464"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH464"/>
    <dbReference type="eggNOG" id="ENOG5033F02">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1314844_0_0_9"/>
    <dbReference type="OrthoDB" id="1175329at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="209" mass="23089" checksum="432A13662F6A00BC" modified="2004-10-11" version="1">MLVSWRVGEGRIFWSADTAWLSNARIGEAQNLDLALQILLPPDGGQVAFDEYHHGFVAPTHWWQVLRGSLQAFGLMLAAAVALLFWSYGARFGSPLPAPERPPRAAVEYVHSMSRLYRRAGARAVVLRALHRSLRTELSRLTGGAEGLSHGEIAGRAAARCGVPAGEIERLLERTADLNHIPADAELIDLARQAEKLRRRIEHATYRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD2</accession>
    <name>Q67SD2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribonuclease G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH426</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194560.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH426"/>
    <dbReference type="EnsemblBacteria" id="BAD39411">
        <property type="protein sequence ID" value="BAD39411"/>
        <property type="gene ID" value="STH426"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH426"/>
    <dbReference type="eggNOG" id="COG1530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003468_5_3_9"/>
    <dbReference type="OMA" id="TKGPRIS"/>
    <dbReference type="OrthoDB" id="1209444at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="39"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="7">
        <location>
            <begin position="504"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="567" mass="63253" checksum="2012D0F318DFAF25" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI6</accession>
    <name>Q67SI6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">mreB</name>
        <name evidence="3" type="ordered locus">STH372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194506.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q67SI6"/>
    <dbReference type="STRING" id="292459.STH372"/>
    <dbReference type="EnsemblBacteria" id="BAD39357">
        <property type="protein sequence ID" value="BAD39357"/>
        <property type="gene ID" value="STH372"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH372"/>
    <dbReference type="eggNOG" id="COG1077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_9"/>
    <dbReference type="OMA" id="MVICIPS"/>
    <dbReference type="OrthoDB" id="410781at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="209"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="289"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="339" mass="36193" checksum="2362FB0FA6CB2650" modified="2004-10-11" version="1">MSFWQFFARDIGIDLGTANTLVYVKGRGIVIDEPSVVAVDRDRKVPLAVGAEAKQMLGRTPGNIVAIRPMKDGVIADFDTTQTMLKYFINRAGSRRAPFRPRIIISIPSGVTGVERRAVQDAALQAGAREAHVIEEPMAAAIGAGLPVEEPTGSMLVDIGGGTTEVAIISLGGLVTAKSIRVAGDELDEAIVQYVKRNYNLLIGERTGEEVKQTIGSAYPTGEEEAMEIRGRDLVTGLPRTLRVTSDEIRKAMAEPVAAIVEAIKITLENTPPELAADIMDRGIVMTGGGALLKGLDVLISKETGMPVHVADEPLLCVVKGCGKCLDNLDILKRVARNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX6</accession>
    <name>Q67SX6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194367.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH232"/>
    <dbReference type="EnsemblBacteria" id="BAD39217">
        <property type="protein sequence ID" value="BAD39217"/>
        <property type="gene ID" value="STH232"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH232"/>
    <dbReference type="eggNOG" id="COG1302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056712_0_0_9"/>
    <dbReference type="OMA" id="RMIDKIT"/>
    <dbReference type="OrthoDB" id="1010640at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="30373" checksum="EC49BBBEDB0B6953" modified="2004-10-11" version="1">MEVVALVGPAGSGKSHRAQIVAYQNQCELIVDDGLLIDKDGKIRAGTSAKREENKMAAVRRAIFLDEGHRAEVRSALWSLKPKKVLVLGTSDEMIVRICDALDLPHPDRTIRIEEIATPAQIRLAQRKRHHEGKHVIPAPTFEVKKTFSGYMVDPLRFFFKRDHGLDPVTLEKSVVRPTFSSLGRFFIADTVIAAIATRAAEMVPGVGRVGRVVVESRREGVLVDMEVLLRYGYHIFDVLREAQEAVAAQIAHMTELNVLEVNLEARRLLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX7</accession>
    <name>Q67SX7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Asparagine synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH231</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194366.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH231"/>
    <dbReference type="EnsemblBacteria" id="BAD39216">
        <property type="protein sequence ID" value="BAD39216"/>
        <property type="gene ID" value="STH231"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH231"/>
    <dbReference type="eggNOG" id="COG0367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_3_2_9"/>
    <dbReference type="OMA" id="DWSGIYS"/>
    <dbReference type="OrthoDB" id="610569at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="377"/>
            <end position="378"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="7">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="614" mass="69029" checksum="3C598CF1DD2A43EF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T08</accession>
    <name>Q67T08_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194335.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39185">
        <property type="protein sequence ID" value="BAD39185"/>
        <property type="gene ID" value="STH200"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH200"/>
    <dbReference type="HOGENOM" id="CLU_1668508_0_0_9"/>
    <dbReference type="OrthoDB" id="2014689at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="158" mass="16876" checksum="BD476C7409887F4F" modified="2004-10-11" version="1">MQSGQVNRSVFWGLTLIAFGLLLLLGNLRIVVWPLRALSGPLALAIPGLIFAAVYSGNRSQWWAIIPAGVMLTLAGVALVDGILPWVNTGWLFFFGLAVTFGLVWRETGGVQRWARVVALACLGMTALILLGSLVRIVLPLALVGIGVYLLVGRGRLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T12</accession>
    <name>GLMS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="1">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmS</name>
        <name type="ordered locus">STH196</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194331.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T12"/>
    <dbReference type="SMR" id="Q67T12"/>
    <dbReference type="STRING" id="292459.STH196"/>
    <dbReference type="PRIDE" id="Q67T12"/>
    <dbReference type="EnsemblBacteria" id="BAD39181">
        <property type="protein sequence ID" value="BAD39181"/>
        <property type="gene ID" value="STH196"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH196"/>
    <dbReference type="eggNOG" id="COG0449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_9"/>
    <dbReference type="OMA" id="ASEYRYA"/>
    <dbReference type="OrthoDB" id="43416at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000135397" description="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]">
        <location>
            <begin position="2"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="SIS 1" evidence="1">
        <location>
            <begin position="286"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="SIS 2" evidence="1">
        <location>
            <begin position="458"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="1">
        <location>
            <position position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <sequence length="609" mass="65839" checksum="A846784F68076A19" modified="2007-01-23" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T43</accession>
    <name>Q67T43_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194300.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39150">
        <property type="protein sequence ID" value="BAD39150"/>
        <property type="gene ID" value="STH165"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH165"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_5_3_9"/>
    <dbReference type="OMA" id="KRWNGVI"/>
    <dbReference type="OrthoDB" id="2057904at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="101" mass="11423" checksum="0D19B98730AFEBC2" modified="2004-10-11" version="1">MAALQLCPKFEHAVHILGKRWTGLILWTLMSGPKRFSEIEAVIPVSGRLLSERLKELESEGLVTREVFPEVPVRVVYSLTPKGQAMEPILAAIQKWAERWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T54</accession>
    <name>Q67T54_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH154"/>
    <dbReference type="EnsemblBacteria" id="BAD39139">
        <property type="protein sequence ID" value="BAD39139"/>
        <property type="gene ID" value="STH154"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH154"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_14_6_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="19964" checksum="E9ECA3818D793E1D" modified="2004-10-11" version="1">MRDAVYFMIVAYRPQVPYDRWGVQELVGKLVQNSIECWVIRRTADAEDVVLLLHVPASPAIPDGFWQPITGGIHQGETAREACVREIFEETGLKVRAGDLRRVPGHWEFDLPHQVIRKELFWVEVTEAAVRTAPEEHDDWRWAPVSAVEGQLYWESNRRTWAAVQAQRYGGEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T56</accession>
    <name>Q67T56_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH152"/>
    <dbReference type="EnsemblBacteria" id="BAD39137">
        <property type="protein sequence ID" value="BAD39137"/>
        <property type="gene ID" value="STH152"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH152"/>
    <dbReference type="eggNOG" id="COG3326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091970_3_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010718">
        <property type="entry name" value="DUF1294"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06961">
        <property type="entry name" value="DUF1294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="10334" checksum="42678D34DC49B270" modified="2004-10-11" version="1">MAGVEGWELLMALAGVCALATFILYGADKYFAVKGRWRVPERTLLLCALLPGGPFGALAGMRLFRHKTRKASFLAINAGGCIVQLLLASLVVDGPTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T58</accession>
    <name>Q67T58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>all-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = 3-heptaprenyl-sn-glycero-1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33495"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58206"/>
            <dbReference type="ChEBI" id="CHEBI:64781"/>
            <dbReference type="EC" id="2.5.1.n9"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194285.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH150"/>
    <dbReference type="EnsemblBacteria" id="BAD39135">
        <property type="protein sequence ID" value="BAD39135"/>
        <property type="gene ID" value="STH150"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH150"/>
    <dbReference type="eggNOG" id="COG1646">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095211_0_0_9"/>
    <dbReference type="OMA" id="TGAHKEW"/>
    <dbReference type="OrthoDB" id="1439634at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002094">
        <property type="term" value="F:polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039074">
        <property type="entry name" value="GGGP/HepGP_synthase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40029">
        <property type="entry name" value="PTHR40029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="25644" checksum="B99532BB2FA3C465" modified="2004-10-11" version="1">MPTSPVTWPPAPDWRAWRHVTKLDPERPLTPAALEAVYASGTDAIVLGGSTGMTQQAVLDLLSRLADAPVPVALEVSTLESAVPGPALFLIPLVMNASDSRWLGGAQAEALARLLPVVGEYIPWELLLPEAYLVLNPDCTAARLTGARTDLAPEDAAGWAALAGQVLRLPLIYVEYSGAFGDMALLQAVRENAGPAHVVYGGGIRSGREAGLAARHAHTIVVGNLVYEAPERLRETVEAAHGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC1</accession>
    <name>ATPF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit b</fullName>
            <shortName evidence="1">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpF</name>
        <name type="ordered locus">STH87</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TC1"/>
    <dbReference type="SMR" id="Q67TC1"/>
    <dbReference type="STRING" id="292459.STH87"/>
    <dbReference type="EnsemblBacteria" id="BAD39072">
        <property type="protein sequence ID" value="BAD39072"/>
        <property type="gene ID" value="STH87"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH87"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_4_4_9"/>
    <dbReference type="OMA" id="FAWKPIL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000368822" description="ATP synthase subunit b">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <sequence length="162" mass="18356" checksum="B17DDF9C3B67FB20" modified="2004-10-11" version="1">MQIFPQTNELIWTIINFAVLLWGMHRFLYKPLLGAIQAREDEINANLKKAAEDRAEAERLRREFEAQIANAQREAQEIINKAVKNATAVKEQIEAEARARAAEILEQATQTIEREKAKAVAELRREVADLAVAVAGKVIEKSLDDAEHRRLADSFVTEVTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG2</accession>
    <name>Q67TG2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH46</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH46"/>
    <dbReference type="EnsemblBacteria" id="BAD39031">
        <property type="protein sequence ID" value="BAD39031"/>
        <property type="gene ID" value="STH46"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH46"/>
    <dbReference type="HOGENOM" id="CLU_2467870_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="10257" checksum="8F950C169C1DE585" modified="2004-10-11" version="1">MFGSGQERPEHSNRRSMSSTVLCEIERLRAIYRAESPTSHFKRRISLILRIDNLRFAKACPRPLVRRCYPQFGRSRQNPVMVDHAASL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ8</accession>
    <name>SYS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">STH10</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194145.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TJ8"/>
    <dbReference type="SMR" id="Q67TJ8"/>
    <dbReference type="STRING" id="292459.STH10"/>
    <dbReference type="EnsemblBacteria" id="BAD38995">
        <property type="protein sequence ID" value="BAD38995"/>
        <property type="gene ID" value="STH10"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH10"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_1_1_9"/>
    <dbReference type="OMA" id="SPCFRRE"/>
    <dbReference type="OrthoDB" id="353391at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000122139" description="Serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="260"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="347"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <sequence length="421" mass="47767" checksum="A82E10E2A8479D9A" modified="2004-10-11" version="1">MLDLRFVRTNPDVVRQALINKGVSVDLDRILALDVERRQILAEVEQLKARRNQVSKQVGILKQQGQDVSAIIAEMGAIGDRIKELDDRERQVSDELQDLLYQLPNLPAPDVPVGPDETGNVEVKRWGEPRQFAFPVKPHWDLGVAMDGLDFERAAKVTGSRFSFIKGGLARLHRALVSFFIDYLTERGYREVLPPVIINTASYYGSGQFPKFKEDVFSLAGTDYHLASTAEVPLVNMHRDEILDEAVLPLRYVGYSGCFRSEAGAAGRDTRGLIRQHYFEKVEMVQFTRPEESEQALMEIVANAEGMLEQLNLPYRRMLMCTGDMGFGQYKKYDIEVWMPSYERYVEISSCSNMSDFQARRANIRYRPAGGKPEFVHTLNGSGLAVGRTLAAVMENYQNEDGSITVPEVLRPYTRCERIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J77</accession>
    <name>Q67J77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Electron transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hydN1</name>
        <name evidence="6" type="ordered locus">STH3292</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197404.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3292"/>
    <dbReference type="EnsemblBacteria" id="BAD42273">
        <property type="protein sequence ID" value="BAD42273"/>
        <property type="gene ID" value="STH3292"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3292"/>
    <dbReference type="eggNOG" id="COG1142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_3_0_9"/>
    <dbReference type="OMA" id="ILCRLCM"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="199" mass="21236" checksum="DF2709DE117EEEFC" modified="2004-10-11" version="1">MTKSAFVLADPTKCIGCQSCEIACAVAHLGTSVTTAGEMKIPFQPRLRVVRTSRVTMPIQCRHCEDAPCANACPVGAIVRQDGVVLVKQERCIGCKTCVLACPFGAMDMVPALENGARVEQPGLKMDTPDGKVPKERYVAHKCDLCIGRPGGPACVEVCLARALTLVRRTAIDQRVRSRRVATASGIARSMSPRAAARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J96</accession>
    <name>Q67J96_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3273"/>
    <dbReference type="EnsemblBacteria" id="BAD42254">
        <property type="protein sequence ID" value="BAD42254"/>
        <property type="gene ID" value="STH3273"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3273"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="23299" checksum="74AA352813E19188" modified="2004-10-11" version="1">MPSATGKGEAAMESTRSRILDAALELISERGYNGATTAEIARRAGVAEGTIYRYFKDKKELFVACVEPVVHEALRRERRLPRSGDLRELIRIRIRELVQIIRENRAVFNVLFTEGRFHPEIADIMLQHVVSAFTAQDREAFAKALGEKPINPQLPIILTVGLAAAIWAILQVGPGVPAPFAGLLPPDWYDRLDDDIADFFTNAIFASATV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB9</accession>
    <name>RSMA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmA</name>
        <name evidence="1" type="synonym">ksgA</name>
        <name type="ordered locus">STH3249</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197362.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JB9"/>
    <dbReference type="SMR" id="Q67JB9"/>
    <dbReference type="STRING" id="292459.STH3249"/>
    <dbReference type="EnsemblBacteria" id="BAD42231">
        <property type="protein sequence ID" value="BAD42231"/>
        <property type="gene ID" value="STH3249"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3249"/>
    <dbReference type="eggNOG" id="COG0030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_0_9"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="OrthoDB" id="2030110at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000101624" description="Ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <sequence length="285" mass="30963" checksum="3AE532C015A43A3E" modified="2004-10-11" version="1">MDLANPGALKALMAQYGLRPQHRLGQNFLIDGRVLDGIVSAAGLEPTDVVLEIGPGLGTLTQRLAAKAGRVVCVELDRGLVQVLHDTVQKAYDNVEVIHGDAGRIDLHKLLGERLAPGQKAKVVANLPYYITTPLVMRLLEEELPLSHVVVMVQKEVADRMVSPPGSKAYGALSVAVQYYTEPRIVLRVSRASFMPQPEVDSAVVSLRYRERPPVDAPPEAFFRVVRAAFGQRRKSLVNALTSLGVEKAAVHAALEAAGIDPGRRGESLSLEEFAAVARTLWQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF3</accession>
    <name>Q67JF3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative glycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197328.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3215"/>
    <dbReference type="EnsemblBacteria" id="BAD42197">
        <property type="protein sequence ID" value="BAD42197"/>
        <property type="gene ID" value="STH3215"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3215"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_9"/>
    <dbReference type="OMA" id="PHIAWAY"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="5">
        <location>
            <begin position="6"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="6">
        <location>
            <begin position="111"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="36345" checksum="4DB0635301FF8D22" modified="2004-10-11" version="1">MAKPRVFVTRRIPDEALSILRTACEVHTWDHEDEPVPQDELVRGLQEADGALVVGPHRIDAALMDAAPRCRVYANMAVGYDNIDVAAATARGILITNTPGVLTETTADLAFGLMIAAARRLYEGQRTIVEGRWKGWSPMFMTGQDVYGATLGIVGAGRIGQAVARRARGFDMRILYHNRRPNPAFEAEVGASYRLLDDLLRESDFVVVLVPLTPETRGLIGARELALMKPTAVLVNAARGPVVDERALYEALRDRRIYAAGLDVFDREPIPADHPLLSLPNVTAVPHIGSATVRTRTRMATLAAENLVAALTGKQPPTPVNPEVLRREERQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH5</accession>
    <name>HUTU_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urocanate hydratase</fullName>
            <shortName evidence="1">Urocanase</shortName>
            <ecNumber evidence="1">4.2.1.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazolonepropionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutU</name>
        <name type="ordered locus">STH3193</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of urocanate to 4-imidazolone-5-propionate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate = H2O + trans-urocanate</text>
            <dbReference type="Rhea" id="RHEA:13101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="4.2.1.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="1">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the urocanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.49" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197306.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JH5"/>
    <dbReference type="SMR" id="Q67JH5"/>
    <dbReference type="STRING" id="292459.STH3193"/>
    <dbReference type="EnsemblBacteria" id="BAD42175">
        <property type="protein sequence ID" value="BAD42175"/>
        <property type="gene ID" value="STH3193"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3193"/>
    <dbReference type="eggNOG" id="COG2987">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018868_0_1_9"/>
    <dbReference type="OMA" id="LVGDWAN"/>
    <dbReference type="OrthoDB" id="100619at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00550"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016153">
        <property type="term" value="F:urocanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00577">
        <property type="entry name" value="HutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023637">
        <property type="entry name" value="Urocanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035401">
        <property type="entry name" value="Urocanase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038364">
        <property type="entry name" value="Urocanase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023636">
        <property type="entry name" value="Urocanase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035400">
        <property type="entry name" value="Urocanase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035085">
        <property type="entry name" value="Urocanase_Rossmann-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036190">
        <property type="entry name" value="Urocanase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12216">
        <property type="entry name" value="PTHR12216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01175">
        <property type="entry name" value="Urocanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17392">
        <property type="entry name" value="Urocanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17391">
        <property type="entry name" value="Urocanase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001423">
        <property type="entry name" value="Urocanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111326">
        <property type="entry name" value="SSF111326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01228">
        <property type="entry name" value="hutU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01233">
        <property type="entry name" value="UROCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000025158" description="Urocanate hydratase">
        <location>
            <begin position="1"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="259"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00577"/>
        </source>
    </evidence>
    <sequence length="551" mass="60753" checksum="9C2DE5B1AA0BD511" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK0</accession>
    <name>Q67JK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">S-layer associated protein, putataive</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3168</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3168"/>
    <dbReference type="EnsemblBacteria" id="BAD42150">
        <property type="protein sequence ID" value="BAD42150"/>
        <property type="gene ID" value="STH3168"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3168"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_317802_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269231" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="35"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="105"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="171"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1101" mass="119177" checksum="C7C2C66646075C8C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN7</accession>
    <name>Q67JN7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cysteine desulfurase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH3131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197244.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3131"/>
    <dbReference type="EnsemblBacteria" id="BAD42113">
        <property type="protein sequence ID" value="BAD42113"/>
        <property type="gene ID" value="STH3131"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3131"/>
    <dbReference type="eggNOG" id="COG0520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_4_9"/>
    <dbReference type="OMA" id="HHVYGGD"/>
    <dbReference type="OrthoDB" id="446447at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010969">
        <property type="entry name" value="Cys_dSase-rel_unknwn_funct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01977">
        <property type="entry name" value="am_tr_V_EF2568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="4">
        <location>
            <begin position="6"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD42113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="389" mass="40700" checksum="33FC9F4B22CA1A40" modified="2004-10-11" version="1">MSAELIYLDNAATSWPKPAAVVRAMAECLENEAGNPGRSGHRLSLAAGRRVWAVRQAVAELFGAPDPLRVIFTHNGTMAVNLALGGLLSPGDRVVCTGMEHNAVIRPLRDLEELGIEVVIAPCDSEGRLDLEAFRRVVEAAPTRMVALNHASNVTGTICPVAEAAAIAHRAGALVLVDAAQSAGSVPIDMGAMGIDLLAFTGHKGLLGPTGTGGLILGERVNAAEMKPLVRGGTGSRSAMERQPPDLPDRFEAGTVNFVGIAGLGAGIQALAELGGPEEIGRRERQLARQLWEGLSEIPGVRLYGPADFSQRVAVVSFTVEGRTVSEVGGRLDEEFGILARVGLHCAPVAHRTLGTFPQGTVRLSPGPFNTPEQIARAVAAVRRLAEGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV3</accession>
    <name>RL14_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplN</name>
        <name type="ordered locus">STH3065</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197180.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV3"/>
    <dbReference type="SMR" id="Q67JV3"/>
    <dbReference type="STRING" id="292459.STH3065"/>
    <dbReference type="EnsemblBacteria" id="BAD42047">
        <property type="protein sequence ID" value="BAD42047"/>
        <property type="gene ID" value="STH3065"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3065"/>
    <dbReference type="eggNOG" id="COG0093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_2_1_9"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="1799923at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000055732" description="50S ribosomal protein L14">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <sequence length="122" mass="13542" checksum="136881EB9E839B4A" modified="2004-10-11" version="1">MIQVQTRLQVADNTGAKELMCIKVLGGSWRRYANIGDVIVCSVKEASPGGVVKKGDVVRAVVVRTKKGLRREDGSYIRFDENAAVILKDDKEPRGTRIFGPVARELRDKEFMKIISLAPEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX9</accession>
    <name>Q67JX9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 10">Thymidine kinase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tdk</name>
        <name evidence="12" type="ordered locus">STH3038</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>ATP + thymidine = ADP + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 11">Belongs to the thymidine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 8 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197153.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3038"/>
    <dbReference type="EnsemblBacteria" id="BAD42021">
        <property type="protein sequence ID" value="BAD42021"/>
        <property type="gene ID" value="STH3038"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3038"/>
    <dbReference type="eggNOG" id="COG1435">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064400_3_0_9"/>
    <dbReference type="OMA" id="GWLELIC"/>
    <dbReference type="OrthoDB" id="1279539at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00124">
        <property type="entry name" value="Thymidine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020633">
        <property type="entry name" value="Thymidine_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="PTHR11441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035805">
        <property type="entry name" value="TK_cell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00603">
        <property type="entry name" value="TK_CELLULAR_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR035805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="21773" checksum="7B0F42B0CE047D0D" modified="2004-10-11" version="1">MHRNLNYGWIDVITSGSMYAGKTTELIRRVRTFILAKRTAVVFQPRMAKRYTDDEVIVTHDGLRFEAVHTDSPLEILWYAEIHKPDVIGIDEAQFYDVSLVDTVQELANRGHYVIAAGLSQTSEGRPFGCMPQLLALADSITSVYGVCVVCGEPATKPFALTAKTEDVVVGGGEKYEARCRKCWLEGRRARGEQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K03</accession>
    <name>Q67K03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Electron transfer flavoprotein beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197129.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3014"/>
    <dbReference type="EnsemblBacteria" id="BAD41997">
        <property type="protein sequence ID" value="BAD41997"/>
        <property type="gene ID" value="STH3014"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3014"/>
    <dbReference type="eggNOG" id="COG2086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_1_0_9"/>
    <dbReference type="OMA" id="YYVYDAE"/>
    <dbReference type="OrthoDB" id="1027643at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="PTHR21294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETF" evidence="2">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="26778" checksum="52124C2CC0430EB9" modified="2004-10-11" version="1">MEILVLLKQTFDTEAKVTVKDGRVVDDDVTLVINPYDEYAVEEALKIREAVGGTVTVLSVGPARVDEALRRALAMGADEAVAIEPPADADEYVTASLLAAWAKDRKFDLILAGNVSVDTGAGQTALRVAEALGLPHVAAALKIEIAGNTATVHRDAEGDTEIVTVPLPALITAQQGLNEPRYPSVPGIMKAKRKQIARLSPADLGVDVAPKTAVTSVYQAEQNRLNKMIAGTPEEQARELVRLLRNEAKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K09</accession>
    <name>Q67K09_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cheX2</name>
        <name evidence="4" type="ordered locus">STH3008</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197123.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3008"/>
    <dbReference type="EnsemblBacteria" id="BAD41991">
        <property type="protein sequence ID" value="BAD41991"/>
        <property type="gene ID" value="STH3008"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3008"/>
    <dbReference type="eggNOG" id="COG1406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116290_1_0_9"/>
    <dbReference type="OrthoDB" id="1956590at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038756">
        <property type="entry name" value="CheX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028051">
        <property type="entry name" value="CheX-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39452">
        <property type="entry name" value="PTHR39452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13690">
        <property type="entry name" value="CheX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="CheX" evidence="3">
        <location>
            <begin position="44"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="18076" checksum="7176EA06285BDBC9" modified="2004-10-11" version="1">MKVEFLNPFVYAGLKVLASEAGLKSVVSDKPQMRKAIATLHAVNVVIGVMGSVQGLVVYGMELHMAKGIIRAMTGQDFPITDPMAESALGELANLITGYASGTLEQAGWPSRISPPRIVRGTGVRFANSAINMLTVPIITELGQITIYLALREVHPAARATGSEGPTGGMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K10</accession>
    <name>Q67K10_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flagellar basal body protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">flgG</name>
        <name evidence="5" type="ordered locus">STH3007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197122.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3007"/>
    <dbReference type="EnsemblBacteria" id="BAD41990">
        <property type="protein sequence ID" value="BAD41990"/>
        <property type="gene ID" value="STH3007"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3007"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_0_0_9"/>
    <dbReference type="OMA" id="NLYFAIQ"/>
    <dbReference type="OrthoDB" id="947161at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="3">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="4">
        <location>
            <begin position="173"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="25109" checksum="CEEC672C65BF4283" modified="2004-10-11" version="1">MIRGIEASRSGMAVEQLRTDVLADNIANMNTSGFKRATATSVEFDRMLIHRMGDPTDPRSQEVGTLGNGSRVDQVVVDERQGDIYETGRALDLALEGPGWFLAQRPDGSLYRTRNGAFRQMADGTITTAEGYRVLMEAPGGLVPLVVPGVEPTFQADGTILADGMPIGRLALADAAPETRLHSRRLEASNVDLAKEMTDLIVTLRAYQVNQRALRVQDETLARAVTEIGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K22</accession>
    <name>Q67K22_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Flagellar hook protein FlgE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flgE</name>
        <name evidence="7" type="ordered locus">STH2995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197110.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2995"/>
    <dbReference type="EnsemblBacteria" id="BAD41978">
        <property type="protein sequence ID" value="BAD41978"/>
        <property type="gene ID" value="STH2995"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2995"/>
    <dbReference type="eggNOG" id="COG1749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_2_4_9"/>
    <dbReference type="OMA" id="GLQNHQT"/>
    <dbReference type="OrthoDB" id="704242at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.98.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011491">
        <property type="entry name" value="Falgellar_hook_FlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037058">
        <property type="entry name" value="Falgellar_hook_FlgE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07559">
        <property type="entry name" value="FlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="4">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="FlaE" evidence="6">
        <location>
            <begin position="178"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="5">
        <location>
            <begin position="338"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="418" mass="44811" checksum="723163830C332BAB" modified="2004-10-11" version="1">MRSLYSGVSGIRSHQTRMDVIGNNIANVNTTGFKASRVTFTDVFNQTLSSGSALTNPQQIGLGVAVGSTDLITDEGAFQMTGRAFDLAISGAGLFVLKGANDEILYSRAGMFDWNPDGILINPALGMKVQGWMAVDGVIGRTDRATMQDIQIVRGTVSLPQATTKVTFKGNLDAAAEEDSTYETIINAYDSLGRDIPITLRFTKGDGNTWTVQYWDPETEGWSEEHTLDFGSDGRILGPQGPNDDDPRLWTITITDPNDQADDLEITLDFRKITQAYDASGSGGTARSTVELDTRDGRPMGSLEAVYVNELGEIIGRYTNGDSQLLAQIALAHFNNMAGLIKEGNSTFAESPASGVPQIGTAGTGGRGTLVPGNLEGSNVDLAGQFTEMILTQRGYQASARVVSTADDMLQEIINLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K84</accession>
    <name>Q67K84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197047.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2931"/>
    <dbReference type="EnsemblBacteria" id="BAD41914">
        <property type="protein sequence ID" value="BAD41914"/>
        <property type="gene ID" value="STH2931"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2931"/>
    <dbReference type="HOGENOM" id="CLU_183749_1_0_9"/>
    <dbReference type="OMA" id="HRSAMDE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="6851" checksum="601DE89DDBA5577A" modified="2004-10-11" version="1">MKARDTIDLYATLADMKAVDYRSVLALTALIDLLVAKGIITREEMAERASQLDAAGEKSLPVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA6</accession>
    <name>Q67KA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197026.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41892">
        <property type="protein sequence ID" value="BAD41892"/>
        <property type="gene ID" value="STH2909"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2909"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="OrthoDB" id="1752733at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="286" mass="33760" checksum="715787A37EB83928" modified="2004-10-11" version="1">MSRPTLHNALRQLHDKEWVQYRRTGLRTVFCLVNTDDGLRLPSDILLQPLPRPAKWIWAVLRTLDRPVTYQELRSLTGFSLDTIRESIQQLLHSRWLVMEGTARRPMRLKARNPAESKRQVEIEQWEKQFTDGVRSGLSRGQCLLYLIVRMALPGSKILVNARLPGLTNPLTDAELEIDLYLPDYRLGLEYNGPQHYTPTEWFPDEVQFRSQRARDLIKLGLCRERGIDLFVVTLQDLWPDQIWRRLARKAPVREIPDEEWHLVRWLYKHIRRYQESVHRENGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD5</accession>
    <name>Q67KD5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2880"/>
    <dbReference type="ESTHER" id="symth-q67kd5">
        <property type="family name" value="AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41863">
        <property type="protein sequence ID" value="BAD41863"/>
        <property type="gene ID" value="STH2880"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2880"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867995_0_0_9"/>
    <dbReference type="OMA" id="IGFGWHD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017208">
        <property type="entry name" value="UCP037442_abhydr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037442">
        <property type="entry name" value="UCP037442_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="33"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="267" mass="28987" checksum="B7C81CACE3AAEE4E" modified="2004-10-11" version="1">MYAADDFGLTAERVDLTTQDGLRLVAYEVRRPNPKAVIIFLSGIQNPSVTAFFGHARWLAGHGYASLLLEMRAHGESEGDLICLGYREHLDVRAAVDYLRASSAYDGVPIVVYGLSMGGATAINAAGQIPEIAGVISMSAFSSWPDVFADNMALQGAPAWLAAAEKPFVAAYMALRFGPTNAGITPARQIARLGDRPALLIHSRGDSQVPFASFERLVARAPGHVETWVVEGDRHLIIDIDDYEHPERAHDYAATILGFLERHWGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF3</accession>
    <name>Q67KF3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Adenylosuccinate lyase</fullName>
            <shortName evidence="11">ASL</shortName>
            <ecNumber evidence="4 11">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH2860</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196979.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2860"/>
    <dbReference type="EnsemblBacteria" id="BAD41845">
        <property type="protein sequence ID" value="BAD41845"/>
        <property type="gene ID" value="STH2860"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2860"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_9"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="OrthoDB" id="347727at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="12">
        <location>
            <begin position="354"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD41845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="437" mass="48919" checksum="1771D87D8EBA8BF9" modified="2004-10-11" version="1">MIERYTRPRMAELWSAANRFQKWLEVELLACEAWAKLGVIPAEAVAELRARAFTVDEAFVRRVAEIEQTTRHDVIAFTTAVAERIDHPAARYIHYGLTSTDVVDTALSAVLNEAVREIQKGLDGLIAALGRQAVRYKDTPIMGRTHGVHAEPTSFGLKLAVFYAQFRRDADRLEQARRSIAVGKLSGAVGNYGNIDPFVEQYVCERLGLGVADISTQVLQRDRHAHLLTTLAILGSSLDSLATELRLLQKTETREVEEPFSKGQKGSSAMPHKRNPVTLEQISGLSRILRANAIAALENVPLWHERDISHSSVERVILPDSTILADYLLDKMTWIIENLHVYPENMRRSMQASYGLTSSGSVLLSLVEKGLSREQAYAVVQEKAMRAWAEGIHLRRLLEEDPTVQALLTPAELDAAFDLKHHLRHVDTIMKRLGLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG8</accession>
    <name>Q67KG8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2845"/>
    <dbReference type="EnsemblBacteria" id="BAD41830">
        <property type="protein sequence ID" value="BAD41830"/>
        <property type="gene ID" value="STH2845"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2845"/>
    <dbReference type="HOGENOM" id="CLU_3222987_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="44" mass="4642" checksum="81A9BB544885D3B9" modified="2004-10-11" version="1">MMAPLSVVLRRNRYLSEAFLLAFGVCAAIGMALIFVAGLLALGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM3</accession>
    <name>GSA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">STH2790</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196909.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KM3"/>
    <dbReference type="SMR" id="Q67KM3"/>
    <dbReference type="STRING" id="292459.STH2790"/>
    <dbReference type="EnsemblBacteria" id="BAD41775">
        <property type="protein sequence ID" value="BAD41775"/>
        <property type="gene ID" value="STH2790"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2790"/>
    <dbReference type="eggNOG" id="COG0001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_9"/>
    <dbReference type="OMA" id="NFGMVEP"/>
    <dbReference type="OrthoDB" id="493793at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000243626" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <sequence length="433" mass="45940" checksum="EB1021B9102E297C" modified="2004-10-11" version="1">MTARYERSAALYERAVARIVGGVNSPARSFKSVGGGAPVFMARGQGPYLYDVDGNRYIDYIGAFGAGMFGHGHPELVAAVARAAEGGTIYGTPNPWEVELAERIHQALPSMERIRFVTTGTEAVMSAVRVARAFTGRPKIIKMEGCYHGHSDFALIAAGSGPSQLGTPDSAGVTEGVAQDVITVPYNDLDSLGEALDVWGPQVAAVLVEPIVGNFGVAMPKPGYLEGVKRLAHAHGALLIFDEVITGFRVAYGGAQTLLGIEPDLTTLGKIIGGGLPLAAYGGRAEIMDWVAPLGPAYQAGTLAGNPLCMQVALTGLEILSRPGVYERLDADAAYLADGLVRSARSHGHTVQLGRAGSMFTLFFCDEPVVDYRTAQRSDPAKFAAMFRGLLDRGIALAPSRFEAWMLTVQHTRADLEETLQIADEVFAQMAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT5</accession>
    <name>Q67KT5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH iclR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196846.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41712">
        <property type="protein sequence ID" value="BAD41712"/>
        <property type="gene ID" value="STH2727"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2727"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="OrthoDB" id="1752733at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="2">
        <location>
            <begin position="100"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="38905" checksum="E35D2AF9E6623CBF" modified="2004-10-11" version="1">MTVHFLPSQVKVPRHILLHDQLTPAAKVLWAGLQLRPKNQEHLAQLCGLSQASIKPGQTQLERFSLLNPGPFPRADWAFLPVDLLRTKPVHPQAKITYAAIQLLPTFADGVAEATTEQLTQLTGRHPATVRRALRSLVDAGWLQVRRSGRTNVLQILVLNPQLEAQKSMIAQINRRLERAPYLGEAIMREWLTLTVDRDNFEDDASPGFLVNPYTGEELKIDRLYPPDVGFEFNGPQHYGPTDLYPNEEQARRQMGRDLIKQAICTQRGILLVTIHAEDLSLEGMLKRLPDRLPLRSLDHKELVIAHLQSVSRAYRQKVPLPPPVPKQACPPGYATGGNAQQYASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU1</accession>
    <name>Q67KU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2720"/>
    <dbReference type="EnsemblBacteria" id="BAD41705">
        <property type="protein sequence ID" value="BAD41705"/>
        <property type="gene ID" value="STH2720"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2720"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038364_0_0_9"/>
    <dbReference type="OMA" id="AQFYWDE"/>
    <dbReference type="OrthoDB" id="1637053at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015378">
        <property type="entry name" value="Transposase-like_Mu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09299">
        <property type="entry name" value="Mu-transpos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="155"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="462" mass="53976" checksum="BFC55E8FA4D10C90" modified="2004-10-11" version="1">MDEQQRDEIAAFRWQLIAPVVAEDVTRLERRRILQQIARQHHQIPHSDKTRVSVRTLERWIAAYRQAGFDGLKPKVRDDARKGRVITPEILEHAIAARREVPERSVQQIIDTLEKAGVVPPGLLRRSTLSQHLVRAGYSRKAMLSRKRRATFRRFEAAHRNDCWQGDVCHLLNLPHPDRPGKTRKVYLIAFIDDYSRIVYGQMYWEEKLPRLEDCLKRTILRYGLPRRIYVDNGAIYSARHLARICGRLGIRLTHSRPYRPQGRGKIERFFLTVQKSFLPEAQQLIAAGQLATLEQLNELFWAWLEVAYHNRVHGVTKQTPRQRFEGDTEPLRRIDPSALREVFLWEEERVVDQTGCFSLYRNQYEVDPALARRKVTLRYDPYDLSQIQVWHEGKRFADAVPLKLHRTRHRSVSPAPEPAAAAPKTGLNFLTLAKKQHDTERRRRLGETAYTRLTQPKGGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV2</accession>
    <name>Q67KV2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2709"/>
    <dbReference type="EnsemblBacteria" id="BAD41694">
        <property type="protein sequence ID" value="BAD41694"/>
        <property type="gene ID" value="STH2709"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2709"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_684993_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="402" mass="45028" checksum="5679A75C7E8EDB06" modified="2004-10-11" version="1">MAVGKVLLVSSRNLYTANGEWRLVSSRASTLSKAFGIETDALALIRSHHVKNGPRAPQLPGFNCEYVYCNSWGNLPESMRRLLGEVKRRLRTTQYRGVILSGDLVYPLLPWLPVERTLIDLHGVPEEWVEYPNPRLGPAWFRKALFHAAIRLHQWAVPRTAGVLTVTNELAELSVERYGARSTYVVPCVGSHLVVEDPPVIRQRWRQRLGIGNSELAFVYSGGLSPWQLFDETIACYRVWRQHLGSACRLVLFTPDPERSLARVVDSGVPSDEVVSMSLLPEEVVPALHACDVGFVLREDNWTNRVAFPNKFAEYCAAGLLIVTSPGLRAAADVVVTKGIGVLVDPREIEPAALSQYPELREKISTRASNWSGYYDACRSLVNERYSMEKALRPVVDFFLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ2</accession>
    <name>Q67KZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196791.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41654">
        <property type="protein sequence ID" value="BAD41654"/>
        <property type="gene ID" value="STH2669"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2669"/>
    <dbReference type="HOGENOM" id="CLU_2620760_0_0_9"/>
    <dbReference type="OrthoDB" id="1406091at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="9112" checksum="581FF49A265E0472" modified="2004-10-11" version="1">MDAAEIRKYGVEPDFEQYTYEENACSWCGQPVLIIQSKERLPGYRTAATACENCMSKMTKRTRAKEFRWVFLPGWRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L07</accession>
    <name>Q67L07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2654</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196776.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2654"/>
    <dbReference type="EnsemblBacteria" id="BAD41639">
        <property type="protein sequence ID" value="BAD41639"/>
        <property type="gene ID" value="STH2654"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2654"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_9"/>
    <dbReference type="OMA" id="SPGQLMW"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269884" evidence="6">
        <location>
            <begin position="37"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="73"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="29939" checksum="E0EC840A868655EE" modified="2004-10-11" version="1">MTSWRRFARNRLAVAGLVVFLLLVLSALLAPHLAPYDPNLQDWKIRLQPPSAEHPFGTDEFGRDLLSRALYGGRVSLVAGVVPVLIGASVGTLIGLVAGYMGGWWDQVLMRLLDVLLAFPMIFLALAIVGTLGPGLLNAMLAVAVVSLPGYARLVRGQVLALRERDFVVAAQAMGATHRRVLLRHLLPNILSPLLVQATLSVGSAILTTASLSFLGLGTQPPTSDWGEMLASGRQYLPEAWWLAVFPGLFVMLAVLSVNLVGDGLRDYFDPKAKNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L09</accession>
    <name>Q67L09_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2652</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2652"/>
    <dbReference type="EnsemblBacteria" id="BAD41637">
        <property type="protein sequence ID" value="BAD41637"/>
        <property type="gene ID" value="STH2652"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2652"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_9"/>
    <dbReference type="OMA" id="AGPHVWF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269252" evidence="3">
        <location>
            <begin position="23"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="4">
        <location>
            <begin position="83"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="514" mass="55879" checksum="83CA9DDF8EE93AA6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L98</accession>
    <name>Q67L98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196685.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2563"/>
    <dbReference type="EnsemblBacteria" id="BAD41548">
        <property type="protein sequence ID" value="BAD41548"/>
        <property type="gene ID" value="STH2563"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2563"/>
    <dbReference type="eggNOG" id="ENOG5031S9P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066420_1_0_9"/>
    <dbReference type="OMA" id="QESKIEH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014199">
        <property type="entry name" value="Spore_YtxC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08812">
        <property type="entry name" value="YtxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02834">
        <property type="entry name" value="spo_ytxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="34342" checksum="BE98CDE1AD788176" modified="2004-10-11" version="1">MQAITIGTAAGIDEIRQRLDDEFRFLREEGLQVRIGQSHRGSFAFLDCAVDGAGRSSQADALLRHSVASALSDVIVEKWESDLIRKIIRGSYSYFSRDEQDLIADYTGRTLNGGSDSPNGYKVNRKSLILHRLRDYFDTADELVLEGFVNFRLRDYIEELEDAVDRAVDEFLMEREHREFIRLLRHFVEIQEPRIEHVHVLLGPGHAFRLLDDDGGAIRSEALEEFVVEMVESEVSYEDLLISALITLAPRSLTVHLADRPPGRDEALETILGVFGDRTVRCEGCPVCSQATAAACEPSQATLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB6</accession>
    <name>Q67LB6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2545"/>
    <dbReference type="EnsemblBacteria" id="BAD41530">
        <property type="protein sequence ID" value="BAD41530"/>
        <property type="gene ID" value="STH2545"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2545"/>
    <dbReference type="HOGENOM" id="CLU_959521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="290" mass="27982" checksum="F78ECEEA1D7594D0" modified="2004-10-11" version="1">MPLPPAAPQSQLNQPAGLPTGAEAAVHLFPGLTSPAVPAGQPQIQPGVAAQGLPGLAPQIQPGVAAQGLPGPAPQIQPGVAAGGLPGPAPQIQPAIPAGAFPGPSPQVLPGIPVQGSPGPVGQALPGMPDQTSPGPVGQVLPGTASQALPGTPAQGFPGGVPQGLSGMAAAGMPGPVPQKAGDGPARAASGAASQGQPVMAVPAGSDPFWAQRQALAQQLLAQQAVQQQAAMAAMLPDLANQVLGADGAAPPTNGATRPESDAPAPATQRRLRAGTYTTRGLQGGYSTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC8</accession>
    <name>Q67LC8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="6">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sucC</name>
        <name evidence="9" type="ordered locus">STH2533</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196656.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2533"/>
    <dbReference type="EnsemblBacteria" id="BAD41518">
        <property type="protein sequence ID" value="BAD41518"/>
        <property type="gene ID" value="STH2533"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2533"/>
    <dbReference type="eggNOG" id="COG0045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_9"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="316012at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="9"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding; shared with subunit alpha" evidence="6">
        <location>
            <begin position="306"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="373" mass="40058" checksum="472D34B3F5F382D6" modified="2004-10-11" version="1">MKLYEYLAKQMFAEHGVPTGNGILCTTPEEAEEAARKIGPCAIKAQVLVGGRGKAGGIKLANTPEEARERAREILGMNLKGYIVDKVLVDPQIKIEKEYYVSITLDGMAKKPLVMASEYGGVEIESVPHDKIVFEHIDIETGLLPFQAREIARKINLTGDLADQFVRILTGLYQAFRKYDAELVEINPLALTDGGTRLIAADGRFNVEDDALFRHPEFPRVEEGTELEKKVRAIGLAFVQLDGDIAVMANGAGMAMGTLDTLAYYGGRPANFLDAGGGSGVEPTAQAFDALLGTKPKAIFVNIFGGITRCDLVAEAIVQVKKAKGIDVPLVVRMVGTNDVRGREILAEAGIQAYTDMGEAAKAAVEAAYGKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG8</accession>
    <name>Q67LG8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2493"/>
    <dbReference type="EnsemblBacteria" id="BAD41478">
        <property type="protein sequence ID" value="BAD41478"/>
        <property type="gene ID" value="STH2493"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2493"/>
    <dbReference type="HOGENOM" id="CLU_2756365_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="70" mass="7694" checksum="F9468A17A6302088" modified="2004-10-11" version="1">MRGMAVLEEALAAGCQPVALVSHGCLVTLMLRELDPAFGFGDWVRMTTPDVYRATRRDAAWRVDRVGTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ4</accession>
    <name>Q67LJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196590.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2467"/>
    <dbReference type="EnsemblBacteria" id="BAD41452">
        <property type="protein sequence ID" value="BAD41452"/>
        <property type="gene ID" value="STH2467"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2467"/>
    <dbReference type="HOGENOM" id="CLU_1795500_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="14633" checksum="CE72EE11ECB7C2E3" modified="2004-10-11" version="1">MRRRLDLTTPADVFFDPVVQLAALCPICALAALRPEAALAALAPGLVLFNPAINPAAALAAFAAGSVPARRRPHRTGRPAVGPGRPWLHDAGVAWEGDRVAADAGLPAGAGGGSGAWCAFPGAPDAVVVRLGGSRWVFRGDGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK2</accession>
    <name>Q67LK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196582.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2459"/>
    <dbReference type="EnsemblBacteria" id="BAD41444">
        <property type="protein sequence ID" value="BAD41444"/>
        <property type="gene ID" value="STH2459"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2459"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_9"/>
    <dbReference type="OMA" id="HPVYWED"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="33001" checksum="2B5C68006CB07188" modified="2004-10-11" version="1">MDLKLLRTFVTAAELENFHQTAERLYLAQPTVTTHIRQLEHLLGFQLFERVGKRVRLTPAGHRFLPHARRVLATYEQAVNEMTAWWQGYDTRLQLVTSPLVATSVLPGLLKEFTQENPRVEVIITTEVSSAVGQVVASGAAHVGLARAPSTHPDTISTVLYTDPVVLVAAPENAAGAGWMDLIGRHLLLTGNHPIYWDPLLVAVSRRQPHLRTLAVDRVDVTKRLLEEGLGVSFLPLSSVVRELAEGRLVTVPVDMELPTAATYLILPQHREVPDAARQFVQLLKRSFPGGVAPGPQGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP5</accession>
    <name>KATG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Catalase-peroxidase</fullName>
            <shortName evidence="1">CP</shortName>
            <ecNumber evidence="1">1.11.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peroxidase/catalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">katG</name>
        <name type="ordered locus">STH2416</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + H2O2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H2O2 = 2 H2O + O2</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="1">Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxidase family. Peroxidase/catalase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.21" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196539.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LP5"/>
    <dbReference type="SMR" id="Q67LP5"/>
    <dbReference type="STRING" id="292459.STH2416"/>
    <dbReference type="PeroxiBase" id="2350">
        <property type="entry name" value="SthCP01_IAM14863"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41401">
        <property type="protein sequence ID" value="BAD41401"/>
        <property type="gene ID" value="STH2416"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2416"/>
    <dbReference type="eggNOG" id="COG0376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025424_2_0_9"/>
    <dbReference type="OMA" id="MILAGNC"/>
    <dbReference type="OrthoDB" id="49441at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01961">
        <property type="entry name" value="Catal_peroxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000763">
        <property type="entry name" value="Catalase_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555">
        <property type="entry name" value="PTHR30555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00460">
        <property type="entry name" value="BPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="SSF48113"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00198">
        <property type="entry name" value="cat_per_HPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0376">Hydrogen peroxide</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000354940" description="Catalase-peroxidase">
        <location>
            <begin position="1"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-237)" evidence="1">
        <location>
            <begin position="88"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-88)" evidence="1">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01961"/>
        </source>
    </evidence>
    <sequence length="725" mass="81957" checksum="951B04CA6355A898" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU1</accession>
    <name>Q67LU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196493.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2370"/>
    <dbReference type="EnsemblBacteria" id="BAD41355">
        <property type="protein sequence ID" value="BAD41355"/>
        <property type="gene ID" value="STH2370"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2370"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_2_9"/>
    <dbReference type="OMA" id="KSLCVCD"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="105" mass="11943" checksum="65C7C05FB6C5986A" modified="2004-10-11" version="1">MERHVILMKALGDLTRLKILKLIWDRERCVCELYPVLGISQPAVSQHIAKLKAAGLVRERRAGMWVYYQADRERLLEGLKAFRAFLDADLTTIPELCGVDGRGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV5</accession>
    <name>Q67LV5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196479.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2356"/>
    <dbReference type="EnsemblBacteria" id="BAD41341">
        <property type="protein sequence ID" value="BAD41341"/>
        <property type="gene ID" value="STH2356"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2356"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072991_1_0_9"/>
    <dbReference type="OrthoDB" id="1791609at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025870">
        <property type="entry name" value="Glyoxalase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13468">
        <property type="entry name" value="Glyoxalase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="171"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="31915" checksum="902DBA1C0BB4605D" modified="2004-10-11" version="1">MAITHIDHVLIAVNGLEEAAERYRRLGFFVTEGGEHPGRGTANRLAVLDPEYLELIAVRDRAAAWPDLVEHLDRFGPGLFTFALASDDLEADLAAFRRRAADGVTDLRPEEPWDGELVTPGGRRRTWRAFRVAGGRAVNPFLIQHDSEGEEKRRKLAGDGELLPHPLQYGSVSRLAFAFPTLDEGVAFFRDGYGLQPEGDPSVCPSRQADRVFFPLKRGLIEVIAPRGGDSPVAAFVAERGYGVHSVAVTVPDTARAADQLESRGVAVRRLPSGTVQVDPAHTLGVRLSLVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M10</accession>
    <name>Q67M10_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gluconate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196424.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2301"/>
    <dbReference type="EnsemblBacteria" id="BAD41286">
        <property type="protein sequence ID" value="BAD41286"/>
        <property type="gene ID" value="STH2301"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2301"/>
    <dbReference type="eggNOG" id="COG2610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027949_0_2_9"/>
    <dbReference type="OMA" id="MVLPKGH"/>
    <dbReference type="OrthoDB" id="1585405at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015128">
        <property type="term" value="F:gluconate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003474">
        <property type="entry name" value="Glcn_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30354">
        <property type="entry name" value="PTHR30354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02447">
        <property type="entry name" value="GntP_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002746">
        <property type="entry name" value="Gluconate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00791">
        <property type="entry name" value="gntP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="458" mass="47947" checksum="2FA367D6C7792457" modified="2004-10-11" version="1">MASAVSGSQMLLALLIGIVVLVFLILKTKIHAFPALIIAAALIGLVGGMDPVDVSSAITTGFGNTLASIGIVIGFGVMMGRILEISGAAERMATFFLKHLSRRRPEWALAITGYFTSIPIFCDSGYVILAPLAKALSRQSGKSVVTLGISLAVGLVSTHHLVPPTPGPLGTAGIFGADIGKMILWGILFAVPVTIVGVLYAVWLGRRIYQVPAADGEGWERKEWKAELEQAIEIVERKDLPGTALSFAPILVPILLILLNTTLSAVGAGGAWARFLIFLGQPIIAVLVGLLIAIYGLGAKMTRPEVLKEMEKGVQSAGIIMLVTGAGGALGQVIRASGAGDYVAQALANSALPAILLPFVIATGLRLIQGSGTTAMLTAASITAPILAPLGIDPVFAAMSASMGAFVFSYFNDSFYWVVNRFIGIEDVKEQIQVWSVPTTLAWLTPGICLVIANTLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M61</accession>
    <name>Q67M61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196373.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2249"/>
    <dbReference type="EnsemblBacteria" id="BAD41234">
        <property type="protein sequence ID" value="BAD41234"/>
        <property type="gene ID" value="STH2249"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2249"/>
    <dbReference type="eggNOG" id="COG2433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688726_0_0_9"/>
    <dbReference type="OrthoDB" id="1625684at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269264" evidence="1">
        <location>
            <begin position="37"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="301"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="46597" checksum="32192F4039455B0B" modified="2004-10-11" version="1">MGEWEPMKGDHTRRARWLAWLMLAALLVHGPVGAGAEEPLQPEVAEYPAENYEPGPGWPEGWTPPPPPEGMSEQMRAEVEQALRDNPDMYDRYDWWVWDPLPAWTQEYPWYWEEDWMVCGTTIHVYAYPKPDTRLIREVYGRSYGWSRLYWKPCRYEPINSLTEARGLTEEEKLAEGRGIEYEYWMDKSHPGLGRDYGYRHNGSPEEIFVFFNFGNASSHFDTPAYLDTTVNRVRVPIRFISEMMGAEVTWDQAGRRVTIHFPAVTREVVKAVPAPGYDYPDLIHPETHLPDPYLYLLQEQTVSVPERTIVLTIDHPVAVVDGHEVALDAPPVIRNDRTMVPVRFIAEQMGAKVYWVGAEPIYRLDDGTMSGRYQVHIFTPFFPLYEYPSWYLENRAVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC4</accession>
    <name>Q67MC4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196309.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2184"/>
    <dbReference type="EnsemblBacteria" id="BAD41169">
        <property type="protein sequence ID" value="BAD41169"/>
        <property type="gene ID" value="STH2184"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2184"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_0_1_9"/>
    <dbReference type="OMA" id="WITEHES"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="27226" checksum="E68AB24FC52B8775" modified="2004-10-11" version="1">MNRRGWKIGELARRVGITVRTLHHYDHIGLLSPSSSTEAGHRVYSDADVRRLLSIISLKRLGFSLREIKVLLSDAAEDPRAMLQVQLARLDEQIAALTEVRAHVQRLHDHLEVGGSVAIEELMAVAHALTLTGSPHFRRDEVEQVRQKYLGLDPAVRRELFETGRRLVAEFRRYFDAGIAPDAPAVTALARRWKEVTGALLPAGEPLTQAAEQYYRDNPDAGLLYGMDVDLYRYIKQAASRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF8</accession>
    <name>Q67MF8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196275.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2150"/>
    <dbReference type="EnsemblBacteria" id="BAD41135">
        <property type="protein sequence ID" value="BAD41135"/>
        <property type="gene ID" value="STH2150"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2150"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_19_1_9"/>
    <dbReference type="OMA" id="HADQPGM"/>
    <dbReference type="OrthoDB" id="1538300at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="15924" checksum="BAF73AFDBDE087F1" modified="2004-10-11" version="1">MNQAVPQIDVVGAVIENESGEVLCARRAPGSARGGLWEFPGGKIEPGERPEEALRREIREELGCDIVVGEPVAEATHRYPEVVVRLLTFRARLVRGRPVSREHAELRWVPVAALGRLDWAPADLPTVARLQAEAGAPGEGRPVSPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI5</accession>
    <name>Q67MI5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="10" type="ordered locus">STH2123</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2123"/>
    <dbReference type="EnsemblBacteria" id="BAD41108">
        <property type="protein sequence ID" value="BAD41108"/>
        <property type="gene ID" value="STH2123"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2123"/>
    <dbReference type="eggNOG" id="COG2262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019597_1_0_9"/>
    <dbReference type="OMA" id="AEMKTGR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045498">
        <property type="entry name" value="HflX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19275">
        <property type="entry name" value="HflX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="245"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="204"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="251"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="276"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="298"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="464" mass="51844" checksum="3A112741E843ABC6" modified="2004-10-11" version="1">MGLRPFCVAEAAAPARVGPTGEGPGELNRNNDKEWPFMNETELRNRQATAFLVGLELPSAYRDGWSAAESLDELERLVDTAGATVVGREVQSRPAPDVATYIGRGKAEEIELLRRELDFDFVIFDDELTPVQQRNLEKIIECQVLDRTAVILDIFALRARTREAKLQVELAQLGYRLPRLTGKGVELSRQAGSAGTVFNRGPGETKLEVDRRRIRDRMAALRRELEEIKAHRERLRAERRESAVPVVALTGYTNAGKSTLHRVLAGSDVLAEDRLFATLDATTRRVQPAEGEPYLLVDTVGFIHNLPHHLVAAFRSTLEEVNQADLLLHVVDASHPKRAEQMATVHRVLEELGAGDKPTLVVYNKIDRVDPAELDHLMRQTPGAVAVAAALGRNLDRLQAAIQQALHERREVLEVVIPYARSAWLSWVHERGRVLTEEHREDGTYVTVELERGLAARLRAALDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP3</accession>
    <name>Q67MP3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1992 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196190.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2065"/>
    <dbReference type="EnsemblBacteria" id="BAD41050">
        <property type="protein sequence ID" value="BAD41050"/>
        <property type="gene ID" value="STH2065"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2065"/>
    <dbReference type="HOGENOM" id="CLU_109304_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DUF1992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1992" evidence="2">
        <location>
            <begin position="19"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="128" mass="15215" checksum="C884B2C2152EB53C" modified="2004-10-11" version="1">MTDDRERNTPPPQKFSAGWLDEIITDAMRRGEFDNLPGKGKPIDLDRDEPDPYAKEGSWLVNRILRENKVAPLWIELEKSIREDREWLATHPKDHPERQARIQELNDKIMLFNLNKPRGILDKLRYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ3</accession>
    <name>Q67MQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidoreductase similar to anaerobic dimethyl sulfoxide reductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196181.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2055"/>
    <dbReference type="EnsemblBacteria" id="BAD41040">
        <property type="protein sequence ID" value="BAD41040"/>
        <property type="gene ID" value="STH2055"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2055"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_2_0_9"/>
    <dbReference type="OMA" id="EIACKNE"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="23487" checksum="F020B0D4DE353A04" modified="2004-10-11" version="1">MTTQLGFHVDMEKCIGCKACEAACKDFYRLPPEIRWRRVRSFETGEFPKAIRVHLSLACNHCEDPACMKGCPVEAYTKRADGLVIHDPTACIGCQYCTWTCPYSVPQFDPEQGVVSKCNGCYQLVDQGQLPRCVQACLTGALSFGEVVAHEAQKRQAVRQAPGFPDPSVTRPATRFAWPEKGPQPDRQVRVAPPAGDGTSVGGAAETVVAAAGEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT1</accession>
    <name>Q67MT1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196153.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2027"/>
    <dbReference type="EnsemblBacteria" id="BAD41012">
        <property type="protein sequence ID" value="BAD41012"/>
        <property type="gene ID" value="STH2027"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2027"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073844_0_0_9"/>
    <dbReference type="OMA" id="LRSAKNW"/>
    <dbReference type="OrthoDB" id="1989297at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="36576" checksum="9218A4CC27D3BD7D" modified="2004-10-11" version="1">MDELNRRLAAAAERQARKEKLERQAARVEEEYRSAAARVRELKRQLEKEQRDVEQLESGSLGSLLATLFTDRTERLDRERREAAEALVRYEEARRWAEQLRADLEAIRAEVAELSGAEEEYRSLLAEKERRIRQAQGPAADRLLALEAEEAQARGRAREIAEARAAGTAARSGLGRVLEQLQSAEGWGAWDMFGGGWFSTMVKHSRIDAAEAELQMVRHDLDVFRRELADVGAVVNLPSVDLDGFTRFADYFFDNFFVDWMVQSKIGEARRRVEEARRQVDRLLEWLDGEERRVHEALEEIRRRREQLLTGHTEDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N16</accession>
    <name>Q67N16_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PTS system fructose/mannose-specific IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1942</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196069.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1942"/>
    <dbReference type="EnsemblBacteria" id="BAD40927">
        <property type="protein sequence ID" value="BAD40927"/>
        <property type="gene ID" value="STH1942"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1942"/>
    <dbReference type="eggNOG" id="COG3715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069101_1_1_9"/>
    <dbReference type="OMA" id="ARNALWG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004700">
        <property type="entry name" value="PTS_IIC_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03609">
        <property type="entry name" value="EII-Sor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51106">
        <property type="entry name" value="PTS_EIIC_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="26214" checksum="781BF7ADB2084F53" modified="2004-10-11" version="1">MTYGIGIALLLGIIAYIAVVMNLGASVFWHEALIVGAVTGLIVGDVQMGLTIGATLTLMSLGMWTYGGATIPDFMTGAILGTAFGALPGVGVEGGLAIAVPAALLMTQLDVLGRATTTLFIHGADKAVAEGNVRAVTWWHLLGQLPWGLTRFFPVFLAVWLGAEPVQNFINWMPDHVMNAMRVTGAVLPALGFALLLTMMPLKKYWPFALLGYVLFAYLKVPMIGIALTAVAVGILYLNLKEVRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH5</accession>
    <name>Q67NH5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1783"/>
    <dbReference type="EnsemblBacteria" id="BAD40768">
        <property type="protein sequence ID" value="BAD40768"/>
        <property type="gene ID" value="STH1783"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1783"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_1_2_9"/>
    <dbReference type="OMA" id="SMEFAPF"/>
    <dbReference type="OrthoDB" id="1037615at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="28013" checksum="AE90261B068B8B4C" modified="2004-10-11" version="1">MAERLQKVMARAGVASRRHCEALIQAGRVTVNGQVVTQLGTKVVPGKDVIEVDGRPLGTPETKVYVMLNKPKGYVSTLYDPQGRPKVIDLLGDEVAQRVYPVGRLDFDTEGLLLLTNDGDLAHGLMHPSRMVKKTYIVKVRGVPGPAKLRALQEGIELEDGPTAPAEVKMLDVRPPNSATLSVRIHEGRNRQVRRMFAALGHEVIHLRRTTLGPLKLGDLSVGEWRYLTDQEVADLYRAAGLRLGGRPRRAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM0</accession>
    <name>Q67NM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aluminum resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1738"/>
    <dbReference type="EnsemblBacteria" id="BAD40723">
        <property type="protein sequence ID" value="BAD40723"/>
        <property type="gene ID" value="STH1738"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1738"/>
    <dbReference type="eggNOG" id="COG4100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037803_3_0_9"/>
    <dbReference type="OMA" id="YGHDDLG"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009651">
        <property type="entry name" value="Met_g_lyase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46658">
        <property type="entry name" value="PTHR46658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06838">
        <property type="entry name" value="Met_gamma_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="424" mass="45078" checksum="3391454FB1A04397" modified="2004-10-11" version="1">MNIWSQLIPDCPEPIARAADEALNLVRPVWSRVDELVLRNQARVLAAFQEAGVAEVHFAESTGYGYNDIGREKLDEVFARAFGAEAGLVRVQFASGTHTIATGLFAVLRPGDRLIAAAGAPYDTLQQVIRGKPGSLAEWGVAYEEVPLRADQTVDLEAVAEAVRRPDARCLFIQRSRGYSLRAPLTVAQIGEIIRAAKAANPGIVTVVDNCYGEFVEAQEPPHVGADLTCGSLIKNPGGGIAPTGGYIVGRQDLVERAAARLFAPGIGPEVGANAGVNRLLYQGLFLAPHVTGEALKGAQFTAAFFAHLGFAVRPSYDAYRSDLVQAVELGRPEALIAFCQAVQQASPVDAHVRPEPWDMPGYDDPVIMAAGTFVQGSSIELSADGPVRPPYVAYFQGGLTREHVMLAALRAASDLAAAGILTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM5</accession>
    <name>Q67NM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1733</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1733"/>
    <dbReference type="EnsemblBacteria" id="BAD40718">
        <property type="protein sequence ID" value="BAD40718"/>
        <property type="gene ID" value="STH1733"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1733"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_2_9"/>
    <dbReference type="OMA" id="FWFAACA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="73"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="212" mass="23902" checksum="E9A4101A85F8A7F5" modified="2004-10-11" version="1">MFCPECRTSLQPDARYCHLCGWDSKAAAAARRAAELGRRPAWKRWVSGVSLGIMAFVVLFMLLVPRTDAYAVPQVGSPAPDFEIPALDGGSVRLSDLRGQPVIINFWATWCPPCRKEMPDFQEVYDRYKGDGLQFYAINVGESRVTVRDFMARIGVDLPVLIDAGEEAQSAYNILPIPATFFIDREGIVRAVYQYQMSRAQIEFEVQRLMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY8</accession>
    <name>Q67NY8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40605">
        <property type="protein sequence ID" value="BAD40605"/>
        <property type="gene ID" value="STH1620"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1620"/>
    <dbReference type="HOGENOM" id="CLU_1980470_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="126" mass="13485" checksum="EA0FFF33F2C17B30" modified="2004-10-11" version="1">MTPGPPTGPPGRGMGMRSLLAVIAVAVGIFLLVTVVLPFVGILLAAVLALAGLGVVAYLAAPVLARLPWFRDRIHVEEHPLGRTIRFGAVRVHQRAHARRADDFIDVEGRTIDVEEDLLPPGDGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P04</accession>
    <name>Q67P04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferrichrome ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195733.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1604"/>
    <dbReference type="EnsemblBacteria" id="BAD40589">
        <property type="protein sequence ID" value="BAD40589"/>
        <property type="gene ID" value="STH1604"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1604"/>
    <dbReference type="eggNOG" id="COG4607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_3_1_9"/>
    <dbReference type="OMA" id="KMSAFGP"/>
    <dbReference type="OrthoDB" id="1942521at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01140">
        <property type="entry name" value="FatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033870">
        <property type="entry name" value="FatB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269293" evidence="1">
        <location>
            <begin position="32"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="71"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="35169" checksum="DE642FAE81E752BB" modified="2004-10-11" version="1">MKRKVSLVSMLLALALAVVGCGGGTSTGANNLSTGGSTSPVQTAEPSPAAQDTIVITHQLGETPVPVNPQKVVVFDFGILDTLDLLGVEVAGLPQGNVPPYLAQYTHSKYANVGTLQEPDFEALSALDPDLIIISGRTSTHYEALSELAPTIYLAVDTSNYMESFRKNMETIGAIFQKEEQVAAELKEIEDKIAEVRTKVAGGSEKALILLSNDKSISAYGPGSRFALIHDVLGFPAVDPTIEVSTHGQQVSFEYVKEKNPDYIFVIDRAAVVSGGGQPAKDTFDNDLVKQTNAYKNGKIIYLDPNYWYLSGGGLVSVSAMIDEVAAAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P06</accession>
    <name>Q67P06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ferrichrome ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1602</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195731.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1602"/>
    <dbReference type="EnsemblBacteria" id="BAD40587">
        <property type="protein sequence ID" value="BAD40587"/>
        <property type="gene ID" value="STH1602"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1602"/>
    <dbReference type="eggNOG" id="COG4605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050494_0_0_9"/>
    <dbReference type="OMA" id="LYMLIQT"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="35279" checksum="9941BC49CCC8208A" modified="2004-10-11" version="1">MKHKRNLIILGILLLLALTVVGLYLFVPLRGNIQYILRLRTHKVLAMALTGTAIAYATVVFQTITNNRILTPSIIGMDSVYQLFQTIMVFFWGSTALQFVDQQINFLITVTLMIGFALLLYQVLFRKDGTNLHFLLLMGIVMGTLFGSLTTFLQVLIDPNEYAGLQSRLFASVSRVNTNLLFLGGGLMIPVMLYGLRYVRTLDAIALGRDHAINLGVDYGPVLRRMLIVVAVLMAAATALVGPITFLGLLVANISYHLLDTYRRGPLIAAAVAVGVVFLVGAQLIMERVLAYSTPVSVIVNFIGGMYFLYLVLKERTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P93</accession>
    <name>Q67P93_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4149 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1515"/>
    <dbReference type="EnsemblBacteria" id="BAD40500">
        <property type="protein sequence ID" value="BAD40500"/>
        <property type="gene ID" value="STH1515"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1515"/>
    <dbReference type="HOGENOM" id="CLU_1776515_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="DUF4149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4149" evidence="5">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="15578" checksum="3EE46D19F85DE085" modified="2004-10-11" version="1">MGLWVGSAAGFALSAPKIFAAFGPDRQSAGDLAGDIIYLLNNVGLLLGVGALLTLLPRLRSGVNKARLALLLGCLGLALTTWLYIFPQMERAQPPEPIQTYAETDPARVEYNRWHTLSGRVYGAAMVLGVGVIVLGPFAETRGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P95</accession>
    <name>Q67P95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative lipoate-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195643.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1513"/>
    <dbReference type="EnsemblBacteria" id="BAD40498">
        <property type="protein sequence ID" value="BAD40498"/>
        <property type="gene ID" value="STH1513"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1513"/>
    <dbReference type="eggNOG" id="COG0095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098639_0_0_9"/>
    <dbReference type="OrthoDB" id="871298at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="17"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="21973" checksum="EEFC260308F86CF6" modified="2004-10-11" version="1">MQIQPLPYDTTALDRFQREGPFIWLWEPERTEAVLGAGTPETDVDLARCAADGVPVYRRRGGGGAVVLAPGCLVITAAYEASRRRFATQWIGPVAEAVARALRSLGLHGAAVRGMGDVAIDDLKVLGSSLYANRQVALYQGSLLVDPDLDRIARYLPHPSREPDYRRGRSHAEFMTSLVRAGYRGDMAALRAALLAELERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC2</accession>
    <name>Q67PC2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1486"/>
    <dbReference type="EnsemblBacteria" id="BAD40471">
        <property type="protein sequence ID" value="BAD40471"/>
        <property type="gene ID" value="STH1486"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1486"/>
    <dbReference type="eggNOG" id="COG5581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086342_0_1_9"/>
    <dbReference type="OMA" id="RRTWEEK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009926">
        <property type="entry name" value="T3SS_YcgR_PilZN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12945">
        <property type="entry name" value="YcgR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T3SS_YcgR_PilZN" evidence="2">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="109"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="26313" checksum="BCD3EBA999E66699" modified="2004-10-11" version="1">MQSHSRWRGAGPLRLPTINQQLTIYIPTGWWKGRYSTYLEALEDSTIRVAHPMFGSALIPLMPGDEVMVEYVYNGERVGFAAQVLQRVTEETPALILTRPGPGDIRRQQLRNFVRLDISLPIEYAPGGRKAGEEEPELLPGRTVNISGGGALIVTGESYPPGTRLDLVLHLPDRVIPVEAEVVRHVPVAVVPGSGDPVQTRLGVRFTQIEERDREQIIRFIFAEHRRRRRKGLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE4</accession>
    <name>Y1464_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0122 protein STH1464</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1464</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY/ffh. May be a regulatory protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0122 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195594.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PE4"/>
    <dbReference type="SMR" id="Q67PE4"/>
    <dbReference type="STRING" id="292459.STH1464"/>
    <dbReference type="EnsemblBacteria" id="BAD40449">
        <property type="protein sequence ID" value="BAD40449"/>
        <property type="gene ID" value="STH1464"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1464"/>
    <dbReference type="eggNOG" id="COG2739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129218_1_0_9"/>
    <dbReference type="OMA" id="SIQEWQE"/>
    <dbReference type="OrthoDB" id="2008796at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00245">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007394">
        <property type="entry name" value="UPF0122"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40083">
        <property type="entry name" value="PTHR40083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04297">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000071842" description="UPF0122 protein STH1464">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00245"/>
        </source>
    </evidence>
    <sequence length="107" mass="12348" checksum="36556BEC1F17BD6C" modified="2004-10-11" version="1">MKDVQRIALLFDFYGPLLTERQQELIRAYYLEDHSLAEIADADGVSRQAVHELIRRSEAALQEYEQRLGFVAEHQRRLRLLDELQEALDRADLSAARRALAALRAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF1</accession>
    <name>Q67PF1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsY</name>
        <name evidence="7" type="ordered locus">STH1457</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1457"/>
    <dbReference type="EnsemblBacteria" id="BAD40442">
        <property type="protein sequence ID" value="BAD40442"/>
        <property type="gene ID" value="STH1457"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1457"/>
    <dbReference type="eggNOG" id="COG0552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_4_9"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="6">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="124"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="271"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="320" mass="33799" checksum="412D669C6E14E617" modified="2004-10-11" version="1">MPANGGRGVGEGESPVSFFERLKAGLQKTKEALIGQVNTVMSVFRKIDDDLFDELEEALIRGDVGVATSAKLVEELRREARQKGLKNGDELLPLLKELVAERLGSGAAPLRLNEGGLTVILVVGVNGVGKTTTIGKLARHLREDRGLKVVLAAADTFRAAAMDQLKVWGERAGAEVVAGPEGADPAAVAFDGVKAARASGADVLIVDTAGRLHNKAHLMAELGKIARVLGREVPGAPHETLLVLDATTGQNAIQQTRLFKETVPVTGIALTKLDGTAKGGVVVAIADTEQVPVKFIGVGERLDDLQPFDPRQFVEALFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ2</accession>
    <name>Q67PJ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1416"/>
    <dbReference type="EnsemblBacteria" id="BAD40401">
        <property type="protein sequence ID" value="BAD40401"/>
        <property type="gene ID" value="STH1416"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1416"/>
    <dbReference type="HOGENOM" id="CLU_2248740_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="11535" checksum="22951A9E7E91FF38" modified="2004-10-11" version="1">MVPPCLVRSGADPLVPGTIIPSALGNGGVPVYLLDARTRRLVVRHRTDSLVQVRRPDGMRQSFSLRSRVYSNPPSAPACTNRWLSAALRPWVYFSRSSLYNIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM1</accession>
    <name>Q67PM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PspA/IM30 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195517.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1387"/>
    <dbReference type="EnsemblBacteria" id="BAD40372">
        <property type="protein sequence ID" value="BAD40372"/>
        <property type="gene ID" value="STH1387"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1387"/>
    <dbReference type="eggNOG" id="COG1842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145657_0_0_9"/>
    <dbReference type="OrthoDB" id="1200558at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007157">
        <property type="entry name" value="PspA_IM30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088">
        <property type="entry name" value="PTHR31088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="31"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="27527" checksum="224922B707035314" modified="2004-10-11" version="1">MGFFARLARLIRGFFSLFIGGLEERNPEAMLEDLKNQLNAAKVRYHTTTANIIAREKTAAAKLKSAEKELEECRLQIQEAKRLNDRELALQLLIQEEHLEAAYNTALEAYNAIKAEADRAREEFKNFEAEMELKMSQIRQLKVQNELADLKKEMNALRSNYATETGAGRINEGLKQAEELIQGKLAKEEAIAELGQNSLDTRMRSLKGSMARRRAEEKLAALFGDEPAQMEEGRVVKERVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS6</accession>
    <name>Q67PS6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rqc2 homolog RqcH</fullName>
            <shortName evidence="1">RqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="4" type="ordered locus">STH1332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. May add between 1 and at least 8 Ala residues. Binds to stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195463.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1332"/>
    <dbReference type="EnsemblBacteria" id="BAD40317">
        <property type="protein sequence ID" value="BAD40317"/>
        <property type="gene ID" value="STH1332"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1332"/>
    <dbReference type="eggNOG" id="COG1293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022481_2_1_9"/>
    <dbReference type="OMA" id="SKPGFVI"/>
    <dbReference type="OrthoDB" id="713667at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_B">
        <property type="entry name" value="RqcH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043682">
        <property type="entry name" value="RqcH_bacterial"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="3">
        <location>
            <begin position="470"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="439"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="602"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="622" mass="69137" checksum="86ECA533B7575E65" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY7</accession>
    <name>Q67PY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1271"/>
    <dbReference type="EnsemblBacteria" id="BAD40256">
        <property type="protein sequence ID" value="BAD40256"/>
        <property type="gene ID" value="STH1271"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1271"/>
    <dbReference type="eggNOG" id="COG0724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_8_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="85" mass="9273" checksum="965D79C6644FD35F" modified="2004-10-11" version="1">MIKTLYFGNLPWAVTPEDLEAAVSQYAEVKAARVATDRETGRSRGFGFVEVPAEQAQAVIDALDGAEWAGRVLTVNEAQPRPGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ1</accession>
    <name>Q67PZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycerol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1267</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195398.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1267"/>
    <dbReference type="EnsemblBacteria" id="BAD40252">
        <property type="protein sequence ID" value="BAD40252"/>
        <property type="gene ID" value="STH1267"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1267"/>
    <dbReference type="eggNOG" id="COG0371">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044754_1_0_9"/>
    <dbReference type="OMA" id="ALHGEQC"/>
    <dbReference type="OrthoDB" id="717704at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Fe-ADH" evidence="7">
        <location>
            <begin position="8"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="366" mass="38722" checksum="023B8E07663CD757" modified="2004-10-11" version="1">MSKVIVAPGKYIQGPGELDRLAEHTGQLGAHAFVIADEFVMNLTGDRVTASYAKAGAAVTMERFQGEITQAEIERLTARCKESQADVVVGIGGGKTLDSAKAVAYYCGNLPAVVVPTVASTDAPCSALSVIYKEDGSFERYLFLPSNPAVVVVDTAIIANAPVRLLVAGMGDALATYFEARAAQRAHKLNIVGGHGTQAAMALARLCYDTLLQEGIKAKAAAEAHVITEALERIVEANTYLSGLGFESCGLAAAHAIHNGLSALEETHGAYHGEKVAFGTLAQLVLENAPLDEIEEVIEFCLNVGLPVTLADLGVHTVREEQIRRVAELSCAEGETIFNMPFEVTPEKVYAAILTADRLGHLYKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q78</accession>
    <name>Q67Q78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal peptidase, type I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195311.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1180"/>
    <dbReference type="MEROPS" id="S26.011"/>
    <dbReference type="EnsemblBacteria" id="BAD40165">
        <property type="protein sequence ID" value="BAD40165"/>
        <property type="gene ID" value="STH1180"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1180"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089996_2_2_9"/>
    <dbReference type="OMA" id="VITFMQD"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027245">
        <property type="entry name" value="Sec11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="PTHR10806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="PTHR10806:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="4">
        <location>
            <begin position="39"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="196" mass="21336" checksum="1629E82515CB8CB9" modified="2004-10-11" version="1">MKILLRILSGAVTAVLAIALAGTVLLALAGRRAPDRIPTVFDYKVLTVLSGSMEPAIRTGDAIIVEPLRPEHEIREGDVITFRAADAPDMLITHRVIGIVSVNGEPAAYVTKGDANEAPDLVPVQRSQIVGIHRWRIPYYGYLSDFMHTREGIISLVIVPGVLLIALEVRKIFQVIAEEEKARQADEKPADAQSPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q99</accession>
    <name>RUVC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="1">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvC</name>
        <name type="ordered locus">STH1159</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195290.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q99"/>
    <dbReference type="SMR" id="Q67Q99"/>
    <dbReference type="STRING" id="292459.STH1159"/>
    <dbReference type="EnsemblBacteria" id="BAD40144">
        <property type="protein sequence ID" value="BAD40144"/>
        <property type="gene ID" value="STH1159"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1159"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091257_3_1_9"/>
    <dbReference type="OMA" id="AICHIWR"/>
    <dbReference type="OrthoDB" id="1815080at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225180" description="Crossover junction endodeoxyribonuclease RuvC">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <sequence length="166" mass="17714" checksum="8CE98D24CE11F455" modified="2004-10-11" version="1">MRILGIDPGTARMGYGIVEDAGAGRERAVEYGCLETPPDMRPELRLQALYRGLADLMARHRPDALAVEELFFGRNVTTAIHVGQARGVVLLAAADSGVPVREFTPMQVKMAVTGYGRANKAQVQRMVQALLGLPEIPKPDDAADALAVALAGLHSERLTARLGGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE9</accession>
    <name>Q67QE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1109</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195241.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1109"/>
    <dbReference type="EnsemblBacteria" id="BAD40094">
        <property type="protein sequence ID" value="BAD40094"/>
        <property type="gene ID" value="STH1109"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1109"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_9"/>
    <dbReference type="OMA" id="YMIIMEA"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="79"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="30688" checksum="B77E11F434D11AA6" modified="2004-10-11" version="1">MAFWRRLFANPSAAVGLVVILLYALAALLAPVIAPYGPREMDLANRLRPPAFLEGGSLNHPLGTDQLGYDLFSRILYGARVSLLVGLISVAISLVVGTLLGCIAGYWRGWVDRILSRLADLLMGFPYLLFTIFAMAIIGPGFGNLILALCFKAWVEFFRLARGEMLTENTKEYVEAARALGRRPAAIIFSEVLPNIYHSLLVLATLRMGYMIIMEASLSFLGLGIPPSIPAWGSMVSAGRDQMLNAWWVATMPGLAIVGLVLAINLLGEGLRDVLDPRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG2</accession>
    <name>Q67QG2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium uptake system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195228.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1096"/>
    <dbReference type="EnsemblBacteria" id="BAD40081">
        <property type="protein sequence ID" value="BAD40081"/>
        <property type="gene ID" value="STH1096"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1096"/>
    <dbReference type="eggNOG" id="COG0569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_3_2_9"/>
    <dbReference type="OMA" id="PEYEAGC"/>
    <dbReference type="OrthoDB" id="1768241at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="2">
        <location>
            <begin position="137"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="221" mass="23645" checksum="EB889A38722F6B2B" modified="2004-10-11" version="1">MTRKTIVVVGLGRFGSTVALHLEKMGHEVLGIDANQDLVDHFAPELTHAVACDSTDEEALRALGLRNFDVGVVAIGSDVEASILTTVLLKEHGVPLVVAKARSELHGRTLAKVGADRVIYPEREMGARLAHSLMTGSEIDYIELSPDYTIMEMAAPPALAGRTLREANLRAQFGISVLALKSGEHINAAPLATDVIRPGDTLVLLGSKEGVKQLERTLQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN2</accession>
    <name>Q67QN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative DNA-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1026</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195158.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1026"/>
    <dbReference type="EnsemblBacteria" id="BAD40011">
        <property type="protein sequence ID" value="BAD40011"/>
        <property type="gene ID" value="STH1026"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1026"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_9"/>
    <dbReference type="OMA" id="ITHEAGG"/>
    <dbReference type="OrthoDB" id="1327994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="6">
        <location>
            <begin position="81"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="163" mass="17694" checksum="3D4A606379E21388" modified="2004-10-11" version="1">MEYTCCETRLGPIYVAWTAQGIACVAAGEGEEEAFRARCRALTGQSPVRNDGRRAELERMLQGWLEGEPYDGPVDLSPLSPFDREVLAACRAIPRGEVRTYGELAAAVGRPRAARAVGGALRRNPVPLLIPCHRVVRSDGTIGQYSMGGPEVKRQLLAMEGAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ4</accession>
    <name>Y1004_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transcriptional regulatory protein STH1004</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1004</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TACO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195136.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QQ4"/>
    <dbReference type="SMR" id="Q67QQ4"/>
    <dbReference type="STRING" id="292459.STH1004"/>
    <dbReference type="EnsemblBacteria" id="BAD39989">
        <property type="protein sequence ID" value="BAD39989"/>
        <property type="gene ID" value="STH1004"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1004"/>
    <dbReference type="eggNOG" id="COG0217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062974_2_2_9"/>
    <dbReference type="OMA" id="ETIMYEG"/>
    <dbReference type="OrthoDB" id="1304328at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000175912" description="Probable transcriptional regulatory protein STH1004">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <sequence length="242" mass="26416" checksum="6BF50307A409F2CB" modified="2004-10-11" version="1">MGRKWENIKRSKAKLDQQRGATFSRITKDIMKAAREGGPDPETNFLLKVAVAAARKANMPNENIQRAIARGAGLDSAERFDEIVYEGYGPGGVAIMMNIVTDNRNRTAADVRHIFSKHGGSLGETGCVSWMFKKRGVIEIDRGETPIGEDDLMMIALDAGAEDLVTEEDSYAIYTAPDALNQVMKQLEAAGVPVEKGEVAMVPTTTVAVSGEEAEQLMRLLDLLEEHDDVQNVYTNADISEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR3</accession>
    <name>Q67QR3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2268 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH995"/>
    <dbReference type="EnsemblBacteria" id="BAD39980">
        <property type="protein sequence ID" value="BAD39980"/>
        <property type="gene ID" value="STH995"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH995"/>
    <dbReference type="eggNOG" id="COG5504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979789_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018728">
        <property type="entry name" value="DUF2268"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10026">
        <property type="entry name" value="DUF2268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2268" evidence="1">
        <location>
            <begin position="148"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="31316" checksum="F4AC8C814EB63DB4" modified="2004-10-11" version="1">MVPVRITPVYRDVLLSLEAAELGSPLWPAFYHMAYLPHRAYFDGLADTYGPLLMGQGGLPGIVTRLAPALRRALEPAPGYRMEERVRSVMDRIQPLLPGRMPHVWLATLFFAAPAATIAVGGRPAIALGMERFSPAPPPGPRYWYPPEAVEEMVPHEAAHVARMEALGLPPSPQHLTLLDMVMLEGTALLFTDLLLGRETLATFMDPDRLAWHRAHDAEAVAAAAREFHAGGLTVFARYFAADAPVSGYWVGYSLCRRYLDRHGAGAMQEMLCLPSEEILRRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR8</accession>
    <name>Q67QR8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="6">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">GTP cyclohydrolase I</fullName>
            <shortName evidence="6">GTP-CH-I</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">folE</name>
        <name evidence="8" type="ordered locus">STH990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homopolymer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH990"/>
    <dbReference type="EnsemblBacteria" id="BAD39975">
        <property type="protein sequence ID" value="BAD39975"/>
        <property type="gene ID" value="STH990"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH990"/>
    <dbReference type="eggNOG" id="COG0302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_3_3_9"/>
    <dbReference type="OMA" id="CEHMCMS"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00860">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="7">
        <location>
            <begin position="6"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="187" mass="21039" checksum="F804756825E511BD" modified="2004-10-11" version="1">MDKAKIEQAVRMILEAVGEDPDREGLLETPRRVADFYEEVFAGLHRDPAERLTAIFNEDHEELVIVRDISFESMCEHHLLPFFGKAHVAYIPRSGRVVGLSKLARVVEDFSRRPQLQERLTAQIADLLMEKLNPLGVLVVVEAEHMCMTMRGVRKPGSSTITSAVRGVFQTSEATRAEAMALIRGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR9</accession>
    <name>Q67QR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">D-alanyl-D-alanine carboxypeptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH989"/>
    <dbReference type="EnsemblBacteria" id="BAD39974">
        <property type="protein sequence ID" value="BAD39974"/>
        <property type="gene ID" value="STH989"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH989"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4991">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_482256_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08239">
        <property type="entry name" value="SH3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00287">
        <property type="entry name" value="SH3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51781">
        <property type="entry name" value="SH3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SH3b" evidence="9">
        <location>
            <begin position="118"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="6">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="565" mass="59941" checksum="600E5321F58F1B1E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT6</accession>
    <name>Q67QT6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH972"/>
    <dbReference type="EnsemblBacteria" id="BAD39957">
        <property type="protein sequence ID" value="BAD39957"/>
        <property type="gene ID" value="STH972"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH972"/>
    <dbReference type="eggNOG" id="COG2043">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074324_0_0_9"/>
    <dbReference type="OMA" id="FYYKLGK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28165" checksum="0EAAE1E82F8CF69A" modified="2004-10-11" version="1">MARVLDRHLRTDTFPLGIRVFRGDEPLPERVRRPWRDMGIKIAICQGIGMARRYGWAVAMGPEDLSCPIAQVAFGFKPAIPYYTEGNLAAGMYVETCGAGALTEQEVPKFAPEEAGTVVVAPLARCTFEPETLLIYGNSAQVMRAVTGALWKTGGALTSTTTGRADCADIVIRTAREGRPQFILPCLGDRIFGQTQDHEMAFTIPWSQVPDLLEGLEGTHRGGVRYPIPAFLRYTPAFPATYQELQRRWDEGETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT7</accession>
    <name>Q67QT7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SrfB or ComAB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH971"/>
    <dbReference type="EnsemblBacteria" id="BAD39956">
        <property type="protein sequence ID" value="BAD39956"/>
        <property type="gene ID" value="STH971"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH971"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_13_1_9"/>
    <dbReference type="OMA" id="TTQVWQI"/>
    <dbReference type="OrthoDB" id="2060970at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="43"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14599" checksum="EDA1FEF5E4B1B5FB" modified="2004-10-11" version="1">MQMDRTPYTDGTLLDALGIVLEEVSPERVVATMPVDHRTRQPMGILHGGASVALAETVASIGAAMHAGEGRTAVGMEINANHIRPKREGTVRAEATPVHVGRRSSVWEIRITDEDGRLVCLSRCTLAFVKLDRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV2</accession>
    <name>Q67QV2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acylaminoacyl-peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH956</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH956"/>
    <dbReference type="ESTHER" id="symth-q67qv2">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39941">
        <property type="protein sequence ID" value="BAD39941"/>
        <property type="gene ID" value="STH956"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH956"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_2_1_9"/>
    <dbReference type="OMA" id="YIHGGPK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="4">
        <location>
            <begin position="522"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="739" mass="82252" checksum="4C0BA9468EDA21F5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW9</accession>
    <name>Q67QW9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH939"/>
    <dbReference type="EnsemblBacteria" id="BAD39924">
        <property type="protein sequence ID" value="BAD39924"/>
        <property type="gene ID" value="STH939"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH939"/>
    <dbReference type="eggNOG" id="ENOG5030TRR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1438135_0_0_9"/>
    <dbReference type="OMA" id="HHENGEF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="202" mass="23070" checksum="50869584243214DF" modified="2004-10-11" version="1">MQVNGGISVGVDLRLIAEARRLLADETPLPFDCGTLCGHRCCTDFGPDEGIYLIPGELPLFDGTEDFTRWQFHSTREYEFAPSWEERFDAVPFLQCTRLCDRTKRPFECRTYPLLPYLRPDGELEMRYSFLARGLCPLPERYRLDELDPAFVEAARRAWALLMEDEAMREHVEWLTAQFDALELPGLDEEGPCGARGGGEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX8</accession>
    <name>Q67QX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH930</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195062.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH930"/>
    <dbReference type="EnsemblBacteria" id="BAD39915">
        <property type="protein sequence ID" value="BAD39915"/>
        <property type="gene ID" value="STH930"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH930"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023342_2_1_9"/>
    <dbReference type="OMA" id="ACYSARG"/>
    <dbReference type="OrthoDB" id="151647at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015930">
        <property type="term" value="F:glutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_synthase" evidence="4">
        <location>
            <begin position="106"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="462"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="481" mass="51179" checksum="F36CBDE8882D0604" modified="2004-10-11" version="1">MLRLIEKWLRAAIDETVDRALARVVRDEYPESIGEMVGALRKVGGLNALEITMRAAQGAPAPRPLGSPLRLSPWDKLLFNPVHLCRFPTPENVPIDTAVTIGPRARRPLTVAIPVLIAGMSFGGALSKRAKIALARAATAVGTATNTGEAPLLEEERAAARLLIGQYNRGGWLNRPEQYRRVDAIEIQPGQGAQGSTPQRTSARNIGPDFRAAFGLEPGQDAVIHSRLPGVNSQADFIRLVRRLRAETGVPVGVKLAATHHLERELAVALEAGVDFVTVDGAEGGTHGGAPTLQDDVGLPTLYAVARARDFLVRQKAAGDVSLIAAGGLITPGQMLKAMALGADAVYTGTAALMTLIGEQAAEALPFEPPTSLVVYTGKRTGELDVDRAARSLQRFLNACVREMEMVAVTLGRTALSEIGRSDLCTLDPVLARATGVQLGLVAPDRQHRLFPEVAPDRRLPGWAEPPGPPAAEPFSAEPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ9</accession>
    <name>Q67QZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH909"/>
    <dbReference type="EnsemblBacteria" id="BAD39894">
        <property type="protein sequence ID" value="BAD39894"/>
        <property type="gene ID" value="STH909"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH909"/>
    <dbReference type="HOGENOM" id="CLU_3258845_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="42" mass="4501" checksum="FE3AF5C25410E95E" modified="2004-10-11" version="1">MNFWLWIIGWIAASAVVVFALSINGKTESGEALEGGHGHGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R24</accession>
    <name>Q67R24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative general stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH884"/>
    <dbReference type="EnsemblBacteria" id="BAD39869">
        <property type="protein sequence ID" value="BAD39869"/>
        <property type="gene ID" value="STH884"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH884"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153787_1_0_9"/>
    <dbReference type="OMA" id="HSTRCPI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022551">
        <property type="entry name" value="BrxC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11009">
        <property type="entry name" value="DUF2847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04019">
        <property type="entry name" value="B_thiol_YtxJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="10303" checksum="CA51752532AED4A3" modified="2004-10-11" version="1">MFKHSTRCPISAAAHREWSAFLAGPEAERADHFWVRVIQERPVSLALASRVGVPHQSPQVLLIRDGRAVWHASHYAITAGRLKAALDQAAAQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R57</accession>
    <name>COAE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaE</name>
        <name type="ordered locus">STH851</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194983.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67R57"/>
    <dbReference type="SMR" id="Q67R57"/>
    <dbReference type="STRING" id="292459.STH851"/>
    <dbReference type="EnsemblBacteria" id="BAD39836">
        <property type="protein sequence ID" value="BAD39836"/>
        <property type="gene ID" value="STH851"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH851"/>
    <dbReference type="eggNOG" id="COG0237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057180_1_1_9"/>
    <dbReference type="OMA" id="QMDIEQK"/>
    <dbReference type="OrthoDB" id="1515383at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000243350" description="Dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="DPCK" evidence="1">
        <location>
            <begin position="3"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="239" mass="25541" checksum="EB59D59C66C0FACD" modified="2004-10-11" version="1">MRIIGLTGSIASGKSTVSAMLREPGAAVIDADAIVHHLQLPGTPVFESIVREFGPGVVRPDGSLDRQALGRIVFADPGRRRALEAIVHPAVRAEIWRQVEQYRREGRPAVVLDVPLLYESGWDRQVDEVWVVWVDAETQKARLIARSGLSPEEAEARIAAQMSLDEKARRADRIIDNRGSLDRTRAQVEAAWRAACGGEGGEPAAGSSAHHGAGSVDPGAGPCDGPGAAPEAERRGGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB8</accession>
    <name>Q67RB8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194923.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH790"/>
    <dbReference type="EnsemblBacteria" id="BAD39775">
        <property type="protein sequence ID" value="BAD39775"/>
        <property type="gene ID" value="STH790"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH790"/>
    <dbReference type="HOGENOM" id="CLU_2669740_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="8699" checksum="B76ACB4FD43E96DA" modified="2004-10-11" version="1">MASAFRAVLDIWWLMFLVFVPCALLGYLVSLLSPGGRRRRISRSGVRALEQLDRMSGIEFEEFLKALFEEVSKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF6</accession>
    <name>Q67RF6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative UDP-glucuronosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH752</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH752"/>
    <dbReference type="EnsemblBacteria" id="BAD39737">
        <property type="protein sequence ID" value="BAD39737"/>
        <property type="gene ID" value="STH752"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH752"/>
    <dbReference type="eggNOG" id="COG0707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028367_0_0_9"/>
    <dbReference type="OMA" id="VICTHPF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009695">
        <property type="entry name" value="Diacylglyc_glucosyltr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06925">
        <property type="entry name" value="MGDG_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGDG_synth" evidence="6">
        <location>
            <begin position="34"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="5">
        <location>
            <begin position="221"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="399" mass="41849" checksum="51479837514F7CCF" modified="2004-10-11" version="1">MERIIKVCPDLWLPGGGAVPTRILLLSADFGSGHLAAATAIAAACRSLSADCEAVVVQCRSPLLGLVGRAYLWQIRHTPDLYRQLYRLPPGRPLRRLVIRVLSGPVRRALQQYNPDVVVGTHPFPAGAALHLLNRSWPKRVPVVMALTDFAPHGFWIWPGVARYFTASEQAARELVRRGADAAAVRATGIPVRMALAADGTGADRTAGPTAGSSSSGVRRVLVIGGGLGLGPIAAAVDALLSLPRPDLRVTVICGRNEALLDQIRRRHGSDARLTALGFTDQVLDHMREADLLVTKPGGITCAEALALGLPMLLLDPLPGPEEENAAYLAGSGAARVVGVKRLAGAADDLLFRRPERLAAMAAAARQAGHPASALAIAAEVLALADAPRAQVAGITPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF7</accession>
    <name>Q67RF7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH751</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the system for phosphate transport across the cytoplasmic membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194884.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH751"/>
    <dbReference type="EnsemblBacteria" id="BAD39736">
        <property type="protein sequence ID" value="BAD39736"/>
        <property type="gene ID" value="STH751"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH751"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_5_0_9"/>
    <dbReference type="OMA" id="FKKKALC"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011862">
        <property type="entry name" value="Phos-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02136">
        <property type="entry name" value="ptsS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7 11" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027143226" description="Phosphate-binding protein" evidence="11">
        <location>
            <begin position="30"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="12">
        <location>
            <begin position="31"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="30005" checksum="DBAC16822D0E4404" modified="2004-10-11" version="1">MAVLRSRPLAAILLAATLLLAGCGSRPQADPNDPLAGTITASGSTALLPLASLAAEIFMEEHLNVTVNVSGGGSYNGLQQVAVGAVDIGNSDIPATGELADVLVEHRVAIAPFVIIVHPENPVDGLTMEQLAGILRGEITNWQEVGGPDLEIVVVSRQQSSGSRATIVEKVLAGEGDISPQALVQDSNGKVVTTVQYTPGAIGYIDASYHRPSKVKALALDGVAYSPDAVLEGRWPIWTYEYMYTKGEPAGLARAFLEFVLSPEFQEEYVAELGFVPITRETAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ1</accession>
    <name>Q67RJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH717"/>
    <dbReference type="EnsemblBacteria" id="BAD39702">
        <property type="protein sequence ID" value="BAD39702"/>
        <property type="gene ID" value="STH717"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH717"/>
    <dbReference type="HOGENOM" id="CLU_469221_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269635" evidence="3">
        <location>
            <begin position="48"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="556"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="581" mass="60821" checksum="19D9E56F8E025C2E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ4</accession>
    <name>Q67RJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH714</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194847.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH714"/>
    <dbReference type="EnsemblBacteria" id="BAD39699">
        <property type="protein sequence ID" value="BAD39699"/>
        <property type="gene ID" value="STH714"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH714"/>
    <dbReference type="eggNOG" id="COG3764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045680_8_1_9"/>
    <dbReference type="OMA" id="TCYPFRL"/>
    <dbReference type="OrthoDB" id="1022681at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05830">
        <property type="entry name" value="Sortase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042003">
        <property type="entry name" value="Sortase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="226" mass="23586" checksum="5D36E88E8E65C8F3" modified="2004-10-11" version="1">MGNLLLILGALLLLVPAARHAYEELAYRRQAEMPPPSPVMMFDETAPAEERPGAADGTAGTGGGAGATGPGAATAPDGGAGSAAAGVTPLYHIAIPRFGVTYAVGDGVDNAVLEQGPGHYPQTVLPGEVGNAALAGHRTLRGRPSFFYHLDRLEPGDLLLVHYPDRSLTFVVERVFVTDPYDLSVIDPTDQPTLTLTTCDPPGSDEMRLIVQARLQSPLVGMAPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK9</accession>
    <name>Q67RK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194832.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH699"/>
    <dbReference type="EnsemblBacteria" id="BAD39684">
        <property type="protein sequence ID" value="BAD39684"/>
        <property type="gene ID" value="STH699"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH699"/>
    <dbReference type="eggNOG" id="COG4945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_602571_0_0_9"/>
    <dbReference type="OrthoDB" id="1831409at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043724">
        <property type="entry name" value="DUF5666"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18914">
        <property type="entry name" value="DUF5666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269340" evidence="1">
        <location>
            <begin position="30"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="162"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5666" evidence="3">
        <location>
            <begin position="402"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="454" mass="47115" checksum="5AD8AE4AF2E22E07" modified="2004-10-11" version="1">MKLWKKTAALSALVMGVSAAPALATSVPADTPVDATPISAPVEILPIVAPEEAGSFLRLVGPVEWVDLEGGFWAVAGMRLIGDQEEIAQFAGQEVVVEGTEFTGISFHMVPAIEVMSIRLAGEVEAMAPVLRDVGADAPLPREILVNGKPVARELGSPVVADGVLLVPLRAIAEAMGAEVAWDGEGQLVRVGLADRTVIFRIGQQEAEVLDAAVAPAGSARISMAQPAQIVGDRTMISADAITTVFGLRQTEAEEGVMSLVPGDVADLTAPEPVTLPDDAFHDLLTGRIRQVEDGRILLEGPPMANGEPMLIWLTVSDDTQITVGEGAGTAADLQVGAEVIVSLSGPILESFPARGGADSIQVLPAPKADILTGVIKEIEGGRILLEGEPMDSGEPFLAWLAVDENTVITIGDAGATAADLQVGARVEVELTGPMLMSYPAQGGAARIRILPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL9</accession>
    <name>Q67RL9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH689</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194822.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH689"/>
    <dbReference type="EnsemblBacteria" id="BAD39674">
        <property type="protein sequence ID" value="BAD39674"/>
        <property type="gene ID" value="STH689"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH689"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="236" mass="25222" checksum="E9D688EFA08F9745" modified="2004-10-11" version="1">MEVIRAEGLVRRFGRVEAVRGIDLSVRRGECLGLLGHNGAGKSTTVRLLLGLLRPTAGRALVLGLPAGHPWVHARVGYSPETPRFHPFLTARETLDFFRKLSGLRPDQADLDGILDLVGLAGAARERVGGFSKGMLQRLAVAQALVGDPEVLFLDEPTSGLDPAGRVAMRRLIRRLKEAGKTIVLNTHIVSDAAQVADRVAIMKAGRLAAVEPPAGDLEARFLELMGGGEADVVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP0</accession>
    <name>Q67RP0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194801.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH668"/>
    <dbReference type="EnsemblBacteria" id="BAD39653">
        <property type="protein sequence ID" value="BAD39653"/>
        <property type="gene ID" value="STH668"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH668"/>
    <dbReference type="eggNOG" id="ENOG502ZBPB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084726_1_0_9"/>
    <dbReference type="OMA" id="FGVRIDQ"/>
    <dbReference type="OrthoDB" id="1706297at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010147">
        <property type="entry name" value="CRISPR-assoc_prot_CasD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01868">
        <property type="entry name" value="casD_Cas5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="25438" checksum="6863A252D549D83D" modified="2004-10-11" version="1">MYTLLLRLAGPMQSWGTASRFTERDTGMEPSKSGVIGILCAALGKPREERPEDGGRWPSLAELARLRMGVRIDAPGRVGVDFQTAGGGRLGTRPYGVAKADGSKPDSVMSWRYYLEDAAFLVGLESGNRALLERLHVALREPVWPLYLGRKSYVPSSPIFLPDGLREGGLETVLAQYPPLVVSGQERVRVVVDQAEPSGEVRMDVPLDFARRLFGIRYVCSYSIDLPDEAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS6</accession>
    <name>Q67RS6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH632</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194765.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH632"/>
    <dbReference type="EnsemblBacteria" id="BAD39617">
        <property type="protein sequence ID" value="BAD39617"/>
        <property type="gene ID" value="STH632"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH632"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_9"/>
    <dbReference type="OMA" id="PIMNLGF"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="19"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="335"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="377"/>
            <end position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="597" mass="67351" checksum="15B49305A6BE729C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU0</accession>
    <name>Q67RU0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carrier domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH618"/>
    <dbReference type="EnsemblBacteria" id="BAD39603">
        <property type="protein sequence ID" value="BAD39603"/>
        <property type="gene ID" value="STH618"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH618"/>
    <dbReference type="HOGENOM" id="CLU_2526297_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="1">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="84" mass="9307" checksum="BC9DA239625FA68E" modified="2004-10-11" version="1">MAPEVALRPMDVRNEALKLIRERVGDRTIGPATTLTALTEEYETSMEELVEALEAEFGVELSEELLVDVETVGELCSLVARVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV0</accession>
    <name>Q67RV0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH608</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH608"/>
    <dbReference type="EnsemblBacteria" id="BAD39593">
        <property type="protein sequence ID" value="BAD39593"/>
        <property type="gene ID" value="STH608"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH608"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="EMPMMLS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="227" mass="24490" checksum="B96E2900B4CA2BC8" modified="2004-10-11" version="1">MSARPLLEAEGLVKEYPMGVRALDGASLSVGRGEIVGIMGRSGSGKSTLLHILGCLDRPDAGRVWIDGIEVTQLSDAALPKIRLHHLGFVFQAHNLVPTLTALENVALPLRYVRPRPADPYARARELLEAVGLGDRMHHLPGQLSGGQQQRVAIARALVNNPALVLADEPTGSLDSQSARALLRLMRRLCQERGQTFVVVTHDPAVGQVCDRIYHMVDGRVLNAAVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S54</accession>
    <name>DNAK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaK</name>
        <name type="ordered locus">STH504</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="1">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194638.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S54"/>
    <dbReference type="SMR" id="Q67S54"/>
    <dbReference type="STRING" id="292459.STH504"/>
    <dbReference type="PRIDE" id="Q67S54"/>
    <dbReference type="EnsemblBacteria" id="BAD39489">
        <property type="protein sequence ID" value="BAD39489"/>
        <property type="gene ID" value="STH504"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH504"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_4_9"/>
    <dbReference type="OMA" id="ISIKRHM"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000226018" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="579"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="579"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="612" mass="65740" checksum="B353A99FCAAA9599" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S82</accession>
    <name>RS20_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsT</name>
        <name type="ordered locus">STH476</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194610.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S82"/>
    <dbReference type="SMR" id="Q67S82"/>
    <dbReference type="STRING" id="292459.STH476"/>
    <dbReference type="EnsemblBacteria" id="BAD39461">
        <property type="protein sequence ID" value="BAD39461"/>
        <property type="gene ID" value="STH476"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH476"/>
    <dbReference type="eggNOG" id="COG0268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160655_1_0_9"/>
    <dbReference type="OMA" id="GVIHKNA"/>
    <dbReference type="OrthoDB" id="2005443at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000168043" description="30S ribosomal protein S20">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <sequence length="94" mass="10026" checksum="00012BA977767379" modified="2004-10-11" version="1">MANTRSAKKRALVAAKRTARNKMVKSSLRTAIRKFREALGTENAPILLNRAFSALDRAASKGVIHKNTAARKKARLAKALAKATAAQAVAAANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA0</accession>
    <name>Q67SA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39443">
        <property type="protein sequence ID" value="BAD39443"/>
        <property type="gene ID" value="STH458"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH458"/>
    <dbReference type="HOGENOM" id="CLU_2467872_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9365" checksum="7102A888D8CD8BCD" modified="2004-10-11" version="1">MPAGVPVSLWNAEGNELAVMELKQSYPSLVIAVPGLVLGEPYAIHAGEGKAVGVPDDGHFVGWEARRPTWLTGAGVTTAPRTFPGWRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC7</accession>
    <name>RL27_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmA</name>
        <name type="ordered locus">STH431</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194565.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SC7"/>
    <dbReference type="SMR" id="Q67SC7"/>
    <dbReference type="STRING" id="292459.STH431"/>
    <dbReference type="EnsemblBacteria" id="BAD39416">
        <property type="protein sequence ID" value="BAD39416"/>
        <property type="gene ID" value="STH431"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH431"/>
    <dbReference type="eggNOG" id="COG0211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095424_4_0_9"/>
    <dbReference type="OMA" id="GKDHTLH"/>
    <dbReference type="OrthoDB" id="1904463at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000181186" description="50S ribosomal protein L27">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <sequence length="95" mass="10079" checksum="E8FD394B1F62FF5A" modified="2004-10-11" version="1">MFLQLFASKKGVGSTKNGRDSNPKYLGVKKGDGSLVTVGQIIVRQRGTKIHPGANVGRGKDDTLYALADGIVKFGRKGANRKQVSVVPIQLAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE2</accession>
    <name>Q67SE2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH416</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194550.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH416"/>
    <dbReference type="EnsemblBacteria" id="BAD39401">
        <property type="protein sequence ID" value="BAD39401"/>
        <property type="gene ID" value="STH416"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH416"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_9"/>
    <dbReference type="OMA" id="PARMLTT"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="171"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="240"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="458" mass="50064" checksum="7E49CFF00FCF68C5" modified="2004-10-11" version="1">MSLRLRLALFYTGQLVVALLLFSFLMYGVLRWNYIREVDASLYSVAWRVAESMQRYWRIPSLKGLADRSTFILVRTDELVIAQSTDFGTFPVPAEAMAGRATYTTERDVNGEPYRLYTLPVYVNGEPVFWVQVAQPLQLLERVLTTLRPILGAGIAGFALLTGAAAWWLAGRAIAPVQRVASAAESVGQSADLSLRVPYQGPQDEVGRLVHTFNGMLDQLQELYARLATAVDAQKRFVADASHELRTPLTIIRGNIDYLERAGTLDPEALADMKAEAARMSELLEELLAMARADAGQTPELEPLALGPLVRDVCRRAGALPHQVDFVVELPPALDRIMVLGHAEWLRRALLILLDNAFKYTPSGSVTVRAGRQGEGVVLQVIDTGPGIPPEDLPRVFDRFFRGDPARSRGGSGLGLAIASWVAGLHGGRLTAESKVGEGSTFSLWLPVYRPAAGGTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG3</accession>
    <name>Q67SG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pilus subunit protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH395"/>
    <dbReference type="EnsemblBacteria" id="BAD39380">
        <property type="protein sequence ID" value="BAD39380"/>
        <property type="gene ID" value="STH395"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH395"/>
    <dbReference type="eggNOG" id="COG3847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171854_8_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007047">
        <property type="entry name" value="Flp_Fap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04964">
        <property type="entry name" value="Flp_Fap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="63" mass="7021" checksum="7C007E2274213E58" modified="2004-10-11" version="1">MSRIAKGFRRLVVRQEGQGMTEYGLIIALIAVVLITTLTGLNKTLDKTFNKVTTQLNNTVNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG7</accession>
    <name>Q67SG7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sercretion system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194525.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH391"/>
    <dbReference type="EnsemblBacteria" id="BAD39376">
        <property type="protein sequence ID" value="BAD39376"/>
        <property type="gene ID" value="STH391"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH391"/>
    <dbReference type="eggNOG" id="COG4965">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_930428_0_0_9"/>
    <dbReference type="OMA" id="WMRKIIR"/>
    <dbReference type="OrthoDB" id="1002350at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="132"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="32168" checksum="8EB708A3D8147387" modified="2004-10-11" version="1">MQFLAALCTFLAVALFLGGLAGALRRRSRLAERLLRERDPLEQEGTVLPRGPGWLERLEREAKQAGLNLSRRHFLLAFGASGGLAALLALAGDLLGAGACLLLGLIGPLLLIRHRARVRAARFAEQLPETLMLMASVIRAGGTLYQAVQTVVQQAPQPMRGEFERVARAMQLQVPAGDALGLIRDRVGLGEFTSVVVACKVAGEAGGDLDRVLESISRELVEDRQFLRAMEAACAEGKASARLVTAVPLVVVALVSYQNPAYFGEALTDPLGRVLLLGSLCAMALGWLLIRQITDVRTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ9</accession>
    <name>Q67SQ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194434.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH299"/>
    <dbReference type="EnsemblBacteria" id="BAD39284">
        <property type="protein sequence ID" value="BAD39284"/>
        <property type="gene ID" value="STH299"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH299"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_9"/>
    <dbReference type="OMA" id="YIARNAM"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="98"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="324" mass="36112" checksum="47F2B0B18C0DA70C" modified="2004-10-11" version="1">MQKLLISLVTILFAIVCMFLVIQNMPGDPVETLAAEIAQTQNLEMDLAYARAKSMLNYDPDIPVLQRLANYLAGVLRGNLGESLSYHKPVTSLMLGALPWTLLVLTISLFLSFAMGVIVGLFIAWKRKRWLNALLNVWQSIFGSIPNYIVAYLLVFLFAVNLGWLPSRGPYSAEVTPGFNGAFIGDVLQHAILPVLSYFLTTVGGWTVSMRANAIGVLSEDYVTYATARGLPQKRILLQYVGRNSILPMITSLAISFGFMFGGSPLIENLFVYPGVGYYLNVAISRRDYPLMQGMYFMMIVVVVLCGLLAEWLYTVLNPRLRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW5</accession>
    <name>Q67SW5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194378.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH243"/>
    <dbReference type="EnsemblBacteria" id="BAD39228">
        <property type="protein sequence ID" value="BAD39228"/>
        <property type="gene ID" value="STH243"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH243"/>
    <dbReference type="HOGENOM" id="CLU_1757898_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="16942" checksum="FDE4B6A9598016E3" modified="2004-10-11" version="1">MMDLLGRLFGRRQPESPAAAWLRRRAQAEPAEVVVEHFRAIEAHDLEWILATLTPERARLYNSPSTFDRRRLSVKAARVTRVAPAPDAPVARVPGYAEQQVFRVEYELELAPGEARRDPTLAEGPQWAYFLLVRERAGEPWLIADWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ8</accession>
    <name>Q67SZ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194345.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH210"/>
    <dbReference type="EnsemblBacteria" id="BAD39195">
        <property type="protein sequence ID" value="BAD39195"/>
        <property type="gene ID" value="STH210"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH210"/>
    <dbReference type="eggNOG" id="COG2345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078469_2_1_9"/>
    <dbReference type="OMA" id="LCQGHCP"/>
    <dbReference type="OrthoDB" id="1288973at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="23570" checksum="E10E08DA1EF6112F" modified="2004-10-11" version="1">MPRQDETSTRREIINLLRVVGPLTVGELGERLHITHVAVRRHLTSLERDGLVTSVQQRLPMGRPTRVYSLTEAADNLFPKKYSTLTVELLDFLHDTDPALVEAFFQRRREEMVARYGADVNADTLAGRVAQLAEIQQANGYLAQCEQDEDGALVLKEFNCPVHLASRRYPHACDHELAFFKTVLGTENIERLECIAHGGQCCRYRITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA9</accession>
    <name>Q67TA9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH99</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH99"/>
    <dbReference type="EnsemblBacteria" id="BAD39084">
        <property type="protein sequence ID" value="BAD39084"/>
        <property type="gene ID" value="STH99"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH99"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051820_1_0_9"/>
    <dbReference type="OMA" id="YVYETRE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="34"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="33546" checksum="51F218500F9967EE" modified="2004-10-11" version="1">MFPYTAAELQQALQNEGYFLDSLSAKKLWSYLRQGKPVGLRGEPGIGKSQLAEAFARAFGYPLVDMECHSHLEAEEIGISWNGFKQIVDAQAYQRSGSGEPPNLYTLEYLNRTPLLESVLAERPTVVRVDEVDKLNEHTTNFFLRYLDKQELVVHNLDHPGQSRVLKARAPLYIFLTSNEYKRLDTAFMRRTVWLDLQFPSEATLARIIQRSVGVPADFAARVARIVVQLRQLNMEKKPAIAEAIEWARALVDVGDGRITPESVEVTIGFLAKYPEDEAIVKEALRQWYDGFGRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE3</accession>
    <name>Q67TE3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH65</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH65"/>
    <dbReference type="EnsemblBacteria" id="BAD39050">
        <property type="protein sequence ID" value="BAD39050"/>
        <property type="gene ID" value="STH65"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH65"/>
    <dbReference type="eggNOG" id="ENOG5031XS8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112839_0_0_9"/>
    <dbReference type="OMA" id="ELYCECC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="23814" checksum="6FEAA4CAE6A8BC03" modified="2004-10-11" version="1">MATVLIATSVTVALRCPRCGRLELSELSRFALGRAGSQRVECECGHHLLTVGVRPGQVWMQVPCFLCEGTHFRYYSPGQFWQPGLKQIACAETDLQLGVLGDEGAVVEYVRPGLSDLERFMEDDAFDGFFDDPVVMYQVMNQVQELGAQGLLRCRCGSRDVGVDLFPDRLELYCVACGRQRTVPAATEQDLDALMRVAYLEIGGDASSRCRGHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ4</accession>
    <name>Q67TJ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein STH14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH14</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194149.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH14"/>
    <dbReference type="EnsemblBacteria" id="BAD38999">
        <property type="protein sequence ID" value="BAD38999"/>
        <property type="gene ID" value="STH14"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH14"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140930_1_0_9"/>
    <dbReference type="OMA" id="MGNMMKQ"/>
    <dbReference type="OrthoDB" id="1883809at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="PTHR33449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00103">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD38999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="109" mass="11734" checksum="EBAFE6E0174CE70A" modified="2004-10-11" version="1">MFPGGGNMQQIMRQVQKMQKKMAEAQEEIAKMTVEGTAGGGSVRVTVTGDRRVTEVKLAPEVVDPDDIEMLQDLIVAAISDGMKKAEELAEAEMKKVIPGGLPSFPGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J97</accession>
    <name>Q67J97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3272</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197384.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3272"/>
    <dbReference type="EnsemblBacteria" id="BAD42253">
        <property type="protein sequence ID" value="BAD42253"/>
        <property type="gene ID" value="STH3272"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3272"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707733_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269356" evidence="2">
        <location>
            <begin position="32"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="390" mass="40039" checksum="97BE271B74BF9CDE" modified="2004-10-11" version="1">MQIPRSLVLKLAALALLVAAGTASVAAPAAAAPAETGASAPGAPATASAADPASTRVLPLREALSLAERYNPGLRLAALQMEVARTARQAAPAGAPALAPLAAAYLQAQYGLTIPTEAITSQAVGQQATYAYEQAVAQYQTARQQVRAGALQAYVEWQKAVATVEAQRAALARTQTQLANVEAAVAVGSAAPYDLLQVQTALAGQEAALAGAEAMEQGARSALGQVIGVPLAPDVQPEPLALSATEVALPDDLDALIDRAIRRRPDLREAAVQLAGRRQQSGLATDGGAAAEVQAASTYGMALAQARAEITRAWLAARGSLEELKAREKAVEMAREALRLAELRYDAGIATWVEVQSASAAALEAEAGRIQAAANLLVRMMELSHAVGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI0</accession>
    <name>Q67JI0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Dihydropteroate synthase</fullName>
            <shortName evidence="8">DHPS</shortName>
            <ecNumber evidence="4 8">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3188</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="4 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197301.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3188"/>
    <dbReference type="EnsemblBacteria" id="BAD42170">
        <property type="protein sequence ID" value="BAD42170"/>
        <property type="gene ID" value="STH3188"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3188"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_2_9"/>
    <dbReference type="OMA" id="FSIDTYH"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="9">
        <location>
            <begin position="17"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="29989" checksum="08570A7FE2BDCA8E" modified="2004-10-11" version="1">MWRISCRGRSLELGTRSVIMGILNVTPDSFSDGGRWTDPERAVARALHMLEEGADIIDIGGESTRPGYEPVPADEEMRRVLPVIAALRRAAPDAIISVDTQKAAVAEAALGAGADMINDIWGLQGDPEMVQVAARWQCPVVVMHNQHGTEYRELMGDIKAFFRRSLALAAAAGLPGDLIILDPGVGFGKTPLQNLEVIRRLGELRELDRPLLLGVSRKSTIGKVLGGLPPEERLEGTAAAVAIGIMGGADILRVHDVRAMKRVAQVADAIVRPGRGGFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM8</accession>
    <name>Q67JM8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3140"/>
    <dbReference type="EnsemblBacteria" id="BAD42122">
        <property type="protein sequence ID" value="BAD42122"/>
        <property type="gene ID" value="STH3140"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3140"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_1_9"/>
    <dbReference type="OMA" id="KPKIAYR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="9"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="695" mass="75725" checksum="3E7C65540E3D3535" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW8</accession>
    <name>RS13_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsM</name>
        <name type="ordered locus">STH3050</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197165.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW8"/>
    <dbReference type="SMR" id="Q67JW8"/>
    <dbReference type="STRING" id="292459.STH3050"/>
    <dbReference type="EnsemblBacteria" id="BAD42032">
        <property type="protein sequence ID" value="BAD42032"/>
        <property type="gene ID" value="STH3050"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3050"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_1_2_9"/>
    <dbReference type="OMA" id="YRGLRHK"/>
    <dbReference type="OrthoDB" id="1772647at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000230572" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="124" mass="14331" checksum="0AB6CA166C425342" modified="2004-10-11" version="1">MARIAGVDLPRDKRIEAALPYIYGIGWSLSREILKKTGIDPDTRVRDLTEEQVAKLREVIDHEYKVEGDLQREVQMNIKRLIEIGCYRGLRHRRGLPVRGQRTKTNARTRKGPRRTVAGKKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K32</accession>
    <name>Q67K32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197098.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2983"/>
    <dbReference type="EnsemblBacteria" id="BAD41966">
        <property type="protein sequence ID" value="BAD41966"/>
        <property type="gene ID" value="STH2983"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2983"/>
    <dbReference type="HOGENOM" id="CLU_1124074_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="26030" checksum="EC2218C7B9FDD2E0" modified="2004-10-11" version="1">METFLYLVLALAAVGALAYAVYQVTVRQQRMLAEITRLERLTAEVIMSAEALLDEIDQRMARLNDLAAQLEIRAVAEVQAKARSRAKSGTQPQADAPPDGRPPAPAPPDAGDQEAPQPQPERAPEVEAAQQKPKRGRRSRAGAGSTAVPSGPAAGSQQAGGSRQAADSGQFASPGQPSQPAEAPPAEPSPAEPARPQAPADRYGDLRQAVWRLADEGKSPVEIAEALGVPRGEVLLLLNLRGKKAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K33</accession>
    <name>Q67K33_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Negative regulator of flagellin synthesis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">flgM</name>
        <name evidence="9" type="ordered locus">STH2982</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlgM family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197097.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2982"/>
    <dbReference type="EnsemblBacteria" id="BAD41965">
        <property type="protein sequence ID" value="BAD41965"/>
        <property type="gene ID" value="STH2982"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2982"/>
    <dbReference type="eggNOG" id="COG2747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169011_3_3_9"/>
    <dbReference type="OrthoDB" id="1833882at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035890">
        <property type="entry name" value="Anti-sigma-28_factor_FlgM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007412">
        <property type="entry name" value="FlgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031316">
        <property type="entry name" value="FlgM_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04316">
        <property type="entry name" value="FlgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101498">
        <property type="entry name" value="SSF101498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03824">
        <property type="entry name" value="FlgM_jcvi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FlgM" evidence="8">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="10873" checksum="BB48D63004BDA8AD" modified="2004-10-11" version="1">MKIDRRDLSGLPGVQRPGFSRSGREVRRSAGSPGVQPPDQLSISDRSVQVSELKPALAVLPPVRAEVIARLKEQIARGEYRVDPHQLAERLLRLGVIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K44</accession>
    <name>Q67K44_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDT1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2971</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GDT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197086.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2971"/>
    <dbReference type="EnsemblBacteria" id="BAD41954">
        <property type="protein sequence ID" value="BAD41954"/>
        <property type="gene ID" value="STH2971"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2971"/>
    <dbReference type="eggNOG" id="COG2119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140894_3_1_9"/>
    <dbReference type="OMA" id="HLHYIAG"/>
    <dbReference type="OrthoDB" id="2050363at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001727">
        <property type="entry name" value="Gdt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12608">
        <property type="entry name" value="PTHR12608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01169">
        <property type="entry name" value="UPF0016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="92" mass="9977" checksum="CEDF58CF110193F7" modified="2004-10-11" version="1">MEWKSALVTFAIIFLAELGDKTQLMVMSLSARSKAPHMIFLGAAGALIASSLVAVVAGDSFLRHIPVRYLRLGTGAMFMMIGAMLVYRSLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K73</accession>
    <name>Q67K73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aminopeptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2942</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S58 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2942"/>
    <dbReference type="EnsemblBacteria" id="BAD41925">
        <property type="protein sequence ID" value="BAD41925"/>
        <property type="gene ID" value="STH2942"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2942"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_448994_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005321">
        <property type="entry name" value="Peptidase_S58_DmpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36512">
        <property type="entry name" value="PTHR36512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03576">
        <property type="entry name" value="Peptidase_S58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="608" mass="63162" checksum="E25880F0BF19D4A3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K83</accession>
    <name>Q67K83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197048.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2932"/>
    <dbReference type="EnsemblBacteria" id="BAD41915">
        <property type="protein sequence ID" value="BAD41915"/>
        <property type="gene ID" value="STH2932"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2932"/>
    <dbReference type="eggNOG" id="ENOG5032YCI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169738_2_1_9"/>
    <dbReference type="OMA" id="MECASEV"/>
    <dbReference type="OrthoDB" id="2064087at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="7759" checksum="2BA094FCE974D95F" modified="2004-10-11" version="1">MANNNNNDLVVSSARSALEQLKYETAAELGIPNYAQQYKGDLPSRVNGSVGGYMVKKMIALAEQQLNGGTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB4</accession>
    <name>Q67KB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2901"/>
    <dbReference type="EnsemblBacteria" id="BAD41884">
        <property type="protein sequence ID" value="BAD41884"/>
        <property type="gene ID" value="STH2901"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2901"/>
    <dbReference type="HOGENOM" id="CLU_1562097_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="171" mass="19180" checksum="B7C12E0B644CA52A" modified="2004-10-11" version="1">MLQLRLQTAFLLLLGQLLLGGLGWFLAGRWPWIGLIPALVLLWFAWRLGVVFREEAERRTRRYRTAAAAWTAVMSQIPGLLLLPFWAPDWIYSLWQGTFLPVAALLERYRPGLGQVVVPWLWASAAAIVLLFAGAGREVPAPAPPPVRAAPGEWVPARRLADVQKRGVRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ5</accession>
    <name>Q67KJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2818</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196937.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2818"/>
    <dbReference type="EnsemblBacteria" id="BAD41803">
        <property type="protein sequence ID" value="BAD41803"/>
        <property type="gene ID" value="STH2818"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2818"/>
    <dbReference type="eggNOG" id="COG2265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_7_0_9"/>
    <dbReference type="OMA" id="FYAGDMK"/>
    <dbReference type="OrthoDB" id="1421660at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="6">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="459" mass="49752" checksum="E517C41BF5BDE7ED" modified="2004-10-11" version="1">MQETQKPPVRPGQEIEVTVHGLGSSGEGVARYEGLTVFIPGGAPGDRLSARVQEVKRNYARAALVRVEEPSPDRVAPPCPVVGKCGGCQLQHIAYEAQLGLKRQQVVDAVERLGRLSGVTVHPTLGMADPWGYRNKAQFPVGRRSGRVIAGFFAPGSHRIVDIERCAIQHPLANRIMAEVKELAGRFGVPIYDERSHTGVLRHVLARVGRGTGEAMAVLVTNGPHLPRGKDIARELMTRIPELVSVVQNINPARTNVVLGRESRVLAGRDAITDYIGDLQFSISPVSFFQVNPAQTEVLYDKALEYAGLTGSETVIDLYCGIGTISLFLARRCREVVGIEWVEEAVADARENAARNGIGNVRFIAGDAAVEMPRLAAEGVRADVIVLDPPRKGCDGPVLEAIAQVAPRRVVYVSCNPASLARDLGRLAEMGYRTVEVQPVDMFPHTAHVECVARVEREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK8</accession>
    <name>Q67KK8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2805"/>
    <dbReference type="EnsemblBacteria" id="BAD41790">
        <property type="protein sequence ID" value="BAD41790"/>
        <property type="gene ID" value="STH2805"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2805"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_9"/>
    <dbReference type="OMA" id="WLQNRNG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="9">
        <location>
            <begin position="193"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="438" mass="46821" checksum="010D0BA755AE6318" modified="2004-10-11" version="1">MRGVTNGGKEMGGAQNPFLIAQQQVRNAVAALGESEELFELLKAPAHFIEVQIPVRMDDGSLRVFTGYRSQHLTTLGPAKGGIRFHPAVTADEVKALSMWMTFKTSVVGLPYGGGKGGVVVDPRKLSLGELERLSRGYVRAIWPYLGPDKDIPAPDVNTNAQIMGWMTDEYETIVGASCPAVFTGKPLSMGGSLGREEATGRGTVITIREALAYLGLPVAGARVAVQGFGNAGQHAARLLEELGAVLVAASDSRGGIVCPGGMRVADLIAYKQATGSVVGFPGSRATDQFGVLTADCDILVPAALENQIDAEVARSIRARVVAEAANGPTTPDGDHVLAQRKIFLIPDILANAGGVTVSYFEWVQNRNQYYWTAEEVNARLEQRMVASFRDVVAMAERHGAYTRTAAYMYAIHRIAESLRMRGLLRAPARQALRAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ7</accession>
    <name>Q67KQ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196874.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2755"/>
    <dbReference type="EnsemblBacteria" id="BAD41740">
        <property type="protein sequence ID" value="BAD41740"/>
        <property type="gene ID" value="STH2755"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2755"/>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1189425_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="143"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="26181" checksum="B049304BFD25EB2C" modified="2004-10-11" version="1">MPETKYGSRIYLGVLAEADAAGPTAVRVYQDFLGRLRRAAPGVKELRLELEEPAPRKETPGVYECWATLKAVVPHDLTRDGAGNHRDAWRAILRDTFQATCQLNHELVHHTSSRVEITREVFPFEEEARAEAAPVEETPKEMIRVKVLLGGQVYDVEIPKDENLLDGVNAKGVDVKWDCKSGVCDTCQIRVLKGMENLSPVNDREREMLGDKINQGYRLCCQVTAHGPCEFEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR2</accession>
    <name>Q67KR2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Argininosuccinate lyase</fullName>
            <shortName evidence="7">ASAL</shortName>
            <ecNumber evidence="3 7">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argH</name>
        <name evidence="11" type="ordered locus">STH2750</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2750"/>
    <dbReference type="EnsemblBacteria" id="BAD41735">
        <property type="protein sequence ID" value="BAD41735"/>
        <property type="gene ID" value="STH2750"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2750"/>
    <dbReference type="eggNOG" id="COG0165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_3_0_9"/>
    <dbReference type="OMA" id="YTNGVAA"/>
    <dbReference type="OrthoDB" id="751464at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="9">
        <location>
            <begin position="133"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="10">
        <location>
            <begin position="400"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="538" mass="58001" checksum="A7441515F66FE6FA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX0</accession>
    <name>Q67KX0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2691</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196813.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2691"/>
    <dbReference type="EnsemblBacteria" id="BAD41676">
        <property type="protein sequence ID" value="BAD41676"/>
        <property type="gene ID" value="STH2691"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2691"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_9"/>
    <dbReference type="OMA" id="DKVWGER"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="122" mass="13286" checksum="068A9B87218D91FB" modified="2004-10-11" version="1">MVKKILIADADPSRRTLLRATLQDPRYEIIEASGGVQALALRERHRPDLVILDEAMPGVSGMEVCRAIKRDRGAGNTPVILLTEQETSTAIGEAGPDVFLPKPYSPLRLLEAVEKLLSQAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX9</accession>
    <name>Q67KX9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2682</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196804.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2682"/>
    <dbReference type="EnsemblBacteria" id="BAD41667">
        <property type="protein sequence ID" value="BAD41667"/>
        <property type="gene ID" value="STH2682"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2682"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_9"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="4">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32503" checksum="518DEAF4DC70322E" modified="2004-10-11" version="1">MRHLFTFGDLVLDVVATASSALEADTDTPGDVRSAPGGSAANFAVWTRRLGSPVCFATRVGDDLLGRALVDDMRREGVEVHAAVDPVYPTAVLVLFSDGVQRHMMVPRGANHFFGPGDLPEERIRTAGWLHATGYAFFWDSTREALQRALAVAREAGVPVSFDPSSAGFIRRHGLDLPEGIRVLMPNRDEARALTGCADVKDAARELARRADLVAVKLGPEGALLARAGQLTYVPPVAPSAPAVDGTGAGDAWGAALIDGLRRGLDPYRAALRANRLGAEVVTRVGARPALSPEIMRQLEVERDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY7</accession>
    <name>Q67KY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196796.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2674"/>
    <dbReference type="EnsemblBacteria" id="BAD41659">
        <property type="protein sequence ID" value="BAD41659"/>
        <property type="gene ID" value="STH2674"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2674"/>
    <dbReference type="HOGENOM" id="CLU_2774411_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="69" mass="7864" checksum="E622C566C1F8593C" modified="2004-10-11" version="1">MSQTISREEMRQAYLDILERAEDATALRLAIICSDQFPETLKERARLAPSLEALRALLAEHRRRSVPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L03</accession>
    <name>Q67L03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-Methyladenine DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196780.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2658"/>
    <dbReference type="EnsemblBacteria" id="BAD41643">
        <property type="protein sequence ID" value="BAD41643"/>
        <property type="gene ID" value="STH2658"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2658"/>
    <dbReference type="eggNOG" id="COG2818">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083758_1_0_9"/>
    <dbReference type="OMA" id="YVAYHDT"/>
    <dbReference type="OrthoDB" id="1835478at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004597">
        <property type="entry name" value="Tag"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00624">
        <property type="entry name" value="tag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="22299" checksum="21D43EF245F2586F" modified="2004-10-11" version="1">MTRHRCSWASTPLLIAYHDDEWGVPARDDRTLFEYLVLESAQAGLSWVTILQRREGYRRAFADFDPERVARFGPEEIAALLADGSIIRNRRKIEAAVQNARAFLAVREQFGSFAAYIWGFVDGEPRINHWRTPAEVPATSPESVAMSRDLRRRGFTFVGPTICYAYMQAVGMVNDHLVDCFRHAELRAAALGTPPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L28</accession>
    <name>Q67L28_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2633</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196755.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2633"/>
    <dbReference type="EnsemblBacteria" id="BAD41618">
        <property type="protein sequence ID" value="BAD41618"/>
        <property type="gene ID" value="STH2633"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2633"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="VHARGMT"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="8"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="28276" checksum="E9808E4C56A636F0" modified="2004-10-11" version="1">MPVAERILEVVDLVKDYGSRGSVTHALRGVSLAVERGEFVGVMGPSGSGKTTLLNVISTIDTPTSGRVWIGGREVTALGRRELARFRRERLGFIFQDFNLLDTLTLRENIGLALSIAGAPAREIDRRVVAMARRLDLEEALDRFPYQVSGGQKQRAAAARALITGPDLVLADEPTGNLDSRSARSLLEALEELNRELQATILLVTHDPVAASYCRRILFIKDGRLFTELVRADAPRQRFFDRILGVLAELGGGGDDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L48</accession>
    <name>Q67L48_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2613</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196735.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2613"/>
    <dbReference type="EnsemblBacteria" id="BAD41598">
        <property type="protein sequence ID" value="BAD41598"/>
        <property type="gene ID" value="STH2613"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2613"/>
    <dbReference type="eggNOG" id="COG0474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_3_0_9"/>
    <dbReference type="OMA" id="GVHRMAK"/>
    <dbReference type="OrthoDB" id="178322at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="681"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="711"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="785"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="824"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="858"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="8">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="885" mass="95282" checksum="2F1F12ACDBB6E821" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA5</accession>
    <name>KHSE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine kinase</fullName>
            <shortName evidence="1">HK</shortName>
            <shortName evidence="1">HSK</shortName>
            <ecNumber evidence="1">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrB</name>
        <name type="ordered locus">STH2556</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196679.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LA5"/>
    <dbReference type="SMR" id="Q67LA5"/>
    <dbReference type="STRING" id="292459.STH2556"/>
    <dbReference type="EnsemblBacteria" id="BAD41541">
        <property type="protein sequence ID" value="BAD41541"/>
        <property type="gene ID" value="STH2556"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2556"/>
    <dbReference type="eggNOG" id="COG0083">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041243_0_2_9"/>
    <dbReference type="OMA" id="CANRIPH"/>
    <dbReference type="OrthoDB" id="610857at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156621" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <sequence length="324" mass="34022" checksum="D473A01AC0A51E27" modified="2004-10-11" version="1">MVRVSVPATSANLGPGFDTLGVALELRNVIEMDETGIDDVVIEVEGAGAGALEDPGRNMVYQAARLVFQRLGYEPNGLLIREKVAIPVARGMGSSAAAIVGGLVAANALVQKRTGGPGLDREELLRMAVAIEGHPDNVTPALLGGFTVSCMDPDRGPLYLCFPPPRGLRAVVVMPEVQIKGRKTEQSRGVLPAQVSLRDAVYNLNRTALLVAAVAQGRTDLLRVAMQDRLHQPYRAALVPGMRSVFEAALSAGALGVALSGAGPSVIALVAESAEPVALAMEAAFQWAGSNARSLTMDLAREGARVLSGPGREQDMLDRPPHWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB1</accession>
    <name>Q67LB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196673.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2550"/>
    <dbReference type="EnsemblBacteria" id="BAD41535">
        <property type="protein sequence ID" value="BAD41535"/>
        <property type="gene ID" value="STH2550"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2550"/>
    <dbReference type="eggNOG" id="COG1937">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130332_2_3_9"/>
    <dbReference type="OMA" id="YCIDVAN"/>
    <dbReference type="OrthoDB" id="1868412at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="10837" checksum="3F4691C663B4E2FB" modified="2004-10-11" version="1">MAEKGPGVRVPLVRDKEGILARLRKIEGQVRGLQRMVEEDRYCVDILNQVAAVEAALNRVGLMLLEGHVKGCVAAAVRRGEGDEAVAELMDVLRRFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD0</accession>
    <name>Q67LD0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2531</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196654.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2531"/>
    <dbReference type="EnsemblBacteria" id="BAD41516">
        <property type="protein sequence ID" value="BAD41516"/>
        <property type="gene ID" value="STH2531"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2531"/>
    <dbReference type="HOGENOM" id="CLU_2738574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="8396" checksum="6A244587DADE164A" modified="2004-10-11" version="1">MHRPEVGRHYKLVRDFEIHDPDTGTLKHLIRKGEVVKVRKIEEEKDLIYLEGISVPAFFRAFQMHIVPAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH2</accession>
    <name>Q67LH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntP family permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196612.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2489"/>
    <dbReference type="EnsemblBacteria" id="BAD41474">
        <property type="protein sequence ID" value="BAD41474"/>
        <property type="gene ID" value="STH2489"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2489"/>
    <dbReference type="eggNOG" id="COG2610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027949_0_2_9"/>
    <dbReference type="OMA" id="DIRAEVY"/>
    <dbReference type="OrthoDB" id="1585405at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015128">
        <property type="term" value="F:gluconate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003474">
        <property type="entry name" value="Glcn_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30354">
        <property type="entry name" value="PTHR30354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02447">
        <property type="entry name" value="GntP_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002746">
        <property type="entry name" value="Gluconate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="452" mass="46498" checksum="E73C2770B88E8711" modified="2004-10-11" version="1">MASGPILLVILLAAIAFIIYSTAKLKLNPFLALLLTAYGVGFAAGMPATEIVSTITNGFGGLMTSIGLVIILGTIIGVFLEKSGAAIVMADSILKVVGEKRPALAMNLIGYIVSIPVFCDSGFVILSSLNKALSKRTGVSLTALAVALSTGLYATHVLVPPTPGPLAAAGNLGLTNLGLAIMVGLLVAIPTSLAGLFWANRFAKDGKTDKSSPEAALRAWEEMKAQYGRLPGVWAAYAPIVVPIALIALSSVADFPSHPFGEGAVKTVLNFLGTPVTALLIGVFCCLFLVEKIDEKVLNDWIGASLKDAATILIVTGAGGALGKILSTTPIGSYLGETLSTWNLGIFLPFVIAAALKTAQGSSTVALVTTSAIIAPMLPALGFDSEMGRVLMVMAIGAGSMVVSHANDSFFWVVSQFSDMDVATAYRAQTIGTAVTGVVAIVTTALLSLVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS9</accession>
    <name>Q67LS9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41367">
        <property type="protein sequence ID" value="BAD41367"/>
        <property type="gene ID" value="STH2382"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2382"/>
    <dbReference type="HOGENOM" id="CLU_1795499_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="16035" checksum="91F1C67E1FB94405" modified="2004-10-11" version="1">MMIIYLTARPDRGPGLLLGLRPTRAQRRLLGLAPWRRLPVPLPRRRGTCVSLARYRYKRLAPGETAFPIVGSRRQSLRSAPTFFRLRVLMRLPGADSDFTGSGARKFLTGPSGSCGLRLPICPARSSTELSRYETVVGKVRSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW5</accession>
    <name>Q67LW5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2346"/>
    <dbReference type="EnsemblBacteria" id="BAD41331">
        <property type="protein sequence ID" value="BAD41331"/>
        <property type="gene ID" value="STH2346"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2346"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028036_0_0_9"/>
    <dbReference type="OMA" id="DPGPYYL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="451" mass="49305" checksum="05C8A38EFECFB915" modified="2004-10-11" version="1">MTEIRLLTPTGHLGFTMLEPESFLRGMAQGPDFVVADSGSSDIGPYPLGADEPCSPVEWQTHDLEIMLVACRRQGVPMIIGSAADTGTDRGVDTYVQILRTVAERHGLPPFRLAYIYAQVSPDEVRRRLRTGVRIAGLDGRPDLTEADLDRTDRIVAVMGAEPIIAALEAGAEVVICGRSSDPAIFAAPLLWKGLDPATAYYAGKVLECASFCAEPFMGKESVLGVVRPGEVIVEPMHPGQRCTPLSVASHAMYERATPFFEHLPGGVLDMRNCRYEAVDERRCRVTGFAFQPEPTVRVKLEGAGKRGERCLAIVGFRDPDTVARIDAVLDWARNKVEARFGPGGYELHFHVYGRNGVMADLEVVDRPAHELCVVVEGVAPDRRTAAELTAMAARQMFYARLPGTKGTAGTAALMSDEVLPARPAYEWTVNHVMPVQDPRELFRLHLTTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M19</accession>
    <name>Q67M19_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2291"/>
    <dbReference type="EnsemblBacteria" id="BAD41276">
        <property type="protein sequence ID" value="BAD41276"/>
        <property type="gene ID" value="STH2291"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2291"/>
    <dbReference type="eggNOG" id="COG1700">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027714_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018633">
        <property type="entry name" value="DUF2357"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007505">
        <property type="entry name" value="PDDEXK_7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09823">
        <property type="entry name" value="DUF2357"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04411">
        <property type="entry name" value="PDDEXK_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2357" evidence="2">
        <location>
            <begin position="56"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2357" evidence="2">
        <location>
            <begin position="342"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="596" mass="65642" checksum="38FBC7BF551E31CF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M52</accession>
    <name>Q67M52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2258"/>
    <dbReference type="EnsemblBacteria" id="BAD41243">
        <property type="protein sequence ID" value="BAD41243"/>
        <property type="gene ID" value="STH2258"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2258"/>
    <dbReference type="HOGENOM" id="CLU_2669745_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="8773" checksum="7DE7995478A9B956" modified="2004-10-11" version="1">MDELRTTGVPWGVIVGKIKEHLPTEWHDLDNRAYQSVKPFLDETFGQGNWDTEKRPTRDGSRSVTWAFVRNRVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA4</accession>
    <name>Q67MA4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2204"/>
    <dbReference type="EnsemblBacteria" id="BAD41189">
        <property type="protein sequence ID" value="BAD41189"/>
        <property type="gene ID" value="STH2204"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2204"/>
    <dbReference type="eggNOG" id="COG2068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1179725_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="2">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="26256" checksum="6343377FEA0F932A" modified="2004-10-11" version="1">MLAAGESRRMGRPKMLLPWDGGTVLEAVVRSLLAAPVRRVAVVLGHRASEHRAALRQLARESRVLVVENPRYREGMLTSAQCGVAVLLRKIPWGMAALSREETRVATALLPEETRDGTAPLREQTRNGAVLLREEPRDGAEPRREESRNGTTLLREETRNRAVLLREETQDGQALLREETWDGAGLLREETRDGAALFREETRDGAGLLREETQDGQALLREETRDGAGLRREQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK7</accession>
    <name>Q67MK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2101"/>
    <dbReference type="EnsemblBacteria" id="BAD41086">
        <property type="protein sequence ID" value="BAD41086"/>
        <property type="gene ID" value="STH2101"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2101"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2425877_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="10274" checksum="D16271F87EB812E8" modified="2004-10-11" version="1">MLKSVPPFWRPTIKASIGDPDEDREFLIEHSPITYVHQLKVPLMVVQGAMDPRVPRPSPTSWWSGSAPWAGRWSTWSSRTRATASPSVRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM1</accession>
    <name>Q67MM1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2087</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196212.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2087"/>
    <dbReference type="EnsemblBacteria" id="BAD41072">
        <property type="protein sequence ID" value="BAD41072"/>
        <property type="gene ID" value="STH2087"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2087"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_9"/>
    <dbReference type="OMA" id="ARYIHQQ"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="9">
        <location>
            <begin position="144"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="464" mass="51153" checksum="843F48718D9803B2" modified="2004-10-11" version="1">MRTYALLIADDEASFRELLQRRLGRKGYLVKAVGSGTEALEALAEEEFDAAIFDMKMPGLDGIATLRRAREIQPSLPVLILTGHGSIETAVEAIRAGAYDYLTKPCNLAELEAVLEKALERRVLEVENQGLREALRRLGDEVEIVGRSEPIRRLLELTRRVAQGSMPVLIEGESGTGKELIARAVHLWSPRASGPFIPVNAGALPAPLLESELFGHARGAFTGAVAAKPGLVELAEGGTLFLDEIGEMPLEVQAKLLRFLESHEVRRVGETRIRRVDVRIVAATNRCLTDEVNAGRFREDLYYRLNVVTLHVPPLRERREDIPLLVEHFLARLGSAKEMTPDAMEILMQQEFRGNVRELFNVVQRGVILSPDRFIAPEDLGLAVRRARSGQAGAGLEAGPTGRLDGSASETGEPFPTLDEVERAHIARALERTGWNRAQAAQLLGVSVRTLYRKIESYGLRSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN9</accession>
    <name>Q67MN9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2069"/>
    <dbReference type="EnsemblBacteria" id="BAD41054">
        <property type="protein sequence ID" value="BAD41054"/>
        <property type="gene ID" value="STH2069"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2069"/>
    <dbReference type="HOGENOM" id="CLU_2071965_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="118" mass="12558" checksum="52481EF0D99A5C99" modified="2004-10-11" version="1">MGRRPDAGWSCALRRTGEGGGWTGPQDDLGEERFLALQLQRSPHGLHRGPGREGEGEDDPLRVRGQVAEAAVVPSCTCPVRRAEPGSVTGPAARPSPVIRARSAAMATVKCRRMLPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR4</accession>
    <name>Q67MR4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196170.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2044"/>
    <dbReference type="EnsemblBacteria" id="BAD41029">
        <property type="protein sequence ID" value="BAD41029"/>
        <property type="gene ID" value="STH2044"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2044"/>
    <dbReference type="HOGENOM" id="CLU_1045559_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="28979" checksum="3B30ECFC21D70738" modified="2004-10-11" version="1">MADLEQMIREGFARGLAGYGPSDRWAQIAAALDAAPVPARRRIRRARIGTAAAALAAAALLSLLYPPVRAAAVDLVIKGANVLWQGRLGDWLAVIFVEEPPTPTEPAGSDLDAADYRWVETAAEAEALAGFAPPTIRDATGDAGRFSVSIVKVTPDLEWRTLRFNYCENARRYTLSAEGYFAPDEAGRMVRQTQPLVGTTIRRGEDMGDDVQTVSLGDAEALCFTRDFGRGPMRGYCMWQVDGIQIRLNGPDLDSVIRLARRVTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU0</accession>
    <name>Q67MU0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196144.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2018"/>
    <dbReference type="EnsemblBacteria" id="BAD41003">
        <property type="protein sequence ID" value="BAD41003"/>
        <property type="gene ID" value="STH2018"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2018"/>
    <dbReference type="HOGENOM" id="CLU_2300772_0_0_9"/>
    <dbReference type="OrthoDB" id="2168121at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="10408" checksum="1F21DE63010D4EC0" modified="2004-10-11" version="1">MSPEEMWQLVERRRRRFIGTLITAAVLMFVGASAIGQIFGDRYGMITLLVVLIGALLFIWRDWRCPACQKGLGVEFRPRVCPNCGARLAPPGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-01-04" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW0</accession>
    <name>Y1998_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0297 protein STH1998</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1998</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0297 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196125.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MW0"/>
    <dbReference type="SMR" id="Q67MW0"/>
    <dbReference type="STRING" id="292459.STH1998"/>
    <dbReference type="EnsemblBacteria" id="BAD40983">
        <property type="protein sequence ID" value="BAD40983"/>
        <property type="gene ID" value="STH1998"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1998"/>
    <dbReference type="eggNOG" id="COG4472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162466_0_0_9"/>
    <dbReference type="OMA" id="IMSGDPT"/>
    <dbReference type="OrthoDB" id="1939488at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01507">
        <property type="entry name" value="UPF0297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009309">
        <property type="entry name" value="IreB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40067">
        <property type="entry name" value="PTHR40067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06135">
        <property type="entry name" value="IreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037258">
        <property type="entry name" value="DUF965_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000216999" description="UPF0297 protein STH1998">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01507"/>
        </source>
    </evidence>
    <sequence length="86" mass="9984" checksum="E695E14696BA32A5" modified="2004-10-11" version="1">MPGTSDKTVYWRNKPEDRNPGEVLQKVYAALKEKGYSPIDQIVGYLLSGDPTYITSHMGARNLIRRIERDELLEELVRSYLEKLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ1</accession>
    <name>Q67MZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1967"/>
    <dbReference type="EnsemblBacteria" id="BAD40952">
        <property type="protein sequence ID" value="BAD40952"/>
        <property type="gene ID" value="STH1967"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1967"/>
    <dbReference type="HOGENOM" id="CLU_1642872_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="17973" checksum="8C750A466FF84AF8" modified="2004-10-11" version="1">MGDGACCGRNVQWREGDGCTFRLELGGRGRGCARNEQWREGDGCTFRLELGGRGRGCVRTVQWGEGDGCTFRLELGGRGRGCARNVQWGEGDGCTFRLELGGRGRGCVRNVRWREGDGCTFRLELRGRGRRYGQNVQWWASAGAHLVGCTFRRGHDPREDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ9</accession>
    <name>Q67MZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196086.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1959"/>
    <dbReference type="EnsemblBacteria" id="BAD40944">
        <property type="protein sequence ID" value="BAD40944"/>
        <property type="gene ID" value="STH1959"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1959"/>
    <dbReference type="eggNOG" id="ENOG50348T6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_927269_0_0_9"/>
    <dbReference type="OMA" id="DIARSMY"/>
    <dbReference type="OrthoDB" id="2156479at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="300" mass="31795" checksum="E70BA3B099FE0AAB" modified="2004-10-11" version="1">MSLWSLLRTEGRLVWKGRWVALLTLALAAASVWGGPFGTYRYAVRSAERIHALRAAYEAEFAELILDDLVAELQGTLALLHPAMGPNYLLAILAVLGTMVLPIWGAQLVGNEFRHRTAKARAAHVGWGAMVAAKVAWLLLLSAGLAALFAGIGALSGQVTWRAAQDALLLAGEVTPPPLKAPLFAQALTAALGLFFFGLVGLLAALVTRSALAGALVGLALPYVEAFVIGTPEWGWLLPRIAYGNLMVDHFVYLPGGMVGEPLALVPAPAPWVSWAVVAGWTVLACLAALRLGQRQQILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N13</accession>
    <name>Q67N13_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196072.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1945"/>
    <dbReference type="EnsemblBacteria" id="BAD40930">
        <property type="protein sequence ID" value="BAD40930"/>
        <property type="gene ID" value="STH1945"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1945"/>
    <dbReference type="HOGENOM" id="CLU_1767110_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="15538" checksum="9CD0E04A80EA2A77" modified="2004-10-11" version="1">MFVTHGDTIVFRSDRYGRFGTSSRLRPAGDRGDGGWDGLSGRRGWPPEHRPGRSRAGDEDLAASLLRRIGALEERVARLEARLSGDGPRSGGRRADGSGHDRYRGWPGGAADGYEAAGPGSPGVRVTLHAGAGAQVSLGQVRLIEHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB7</accession>
    <name>Q67NB7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative rRNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TlyA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195969.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1841"/>
    <dbReference type="EnsemblBacteria" id="BAD40826">
        <property type="protein sequence ID" value="BAD40826"/>
        <property type="gene ID" value="STH1841"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1841"/>
    <dbReference type="eggNOG" id="COG1189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058015_3_0_9"/>
    <dbReference type="OMA" id="VLMVKPQ"/>
    <dbReference type="OrthoDB" id="1670446at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004538">
        <property type="entry name" value="Haemolysin_A/TlyA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32319:SF0">
        <property type="entry name" value="PTHR32319:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005578">
        <property type="entry name" value="TlyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00478">
        <property type="entry name" value="tly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S4" evidence="2">
        <location>
            <begin position="4"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27248" checksum="0302D9836ED17348" modified="2004-10-11" version="1">MAGERLDVALVSRGHFPSRERAQEAIAAGMVRVNGRVITKPARKVDPGDAIEVTGDPIGYVGRGGLKLQGALDQFGIDPAGRVCLDVGASTGGFTDCLLRRGARRVYAVDVGRDQLHPSLRQDPRVVVMEETDIRSVAALPEPVELAAVDVSFISLTLVLPAVARLLAPAGQIVALIKPQFEVGPGGVGKGCIVRDPAQHRLAVDRVLRRAAELGLACVRVIDSPILGTEGNREFLAWFVPGANLAHREGAAVPDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF1</accession>
    <name>Q67NF1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="3 5">N5-CAIR synthase</shortName>
            <ecNumber evidence="3 5">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 5">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purK</name>
        <name evidence="7" type="ordered locus">STH1807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="3 5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195935.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1807"/>
    <dbReference type="EnsemblBacteria" id="BAD40792">
        <property type="protein sequence ID" value="BAD40792"/>
        <property type="gene ID" value="STH1807"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1807"/>
    <dbReference type="eggNOG" id="COG0026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_1_9"/>
    <dbReference type="OMA" id="ITFDHEH"/>
    <dbReference type="OrthoDB" id="1165275at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="108"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="149"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="264"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="40162" checksum="CF93D574FADCD75F" modified="2004-10-11" version="1">MIRPGEVVGIVGGGQLARMSALAARAMGYRVAVADPDPDCPAAPVADRVIVGALNDPEALAPLADVASVITYEFENIDGAVLDRLAARRPVHPRVEILRTCQNRISEKQALERLGIPVAPWRPVRSADEIPAALEAVGLPAVLKTATMGYDGKGQAVIRTTAEAERALEALGGGPLVLERLVDFACELSVVVARSASGHVAPFPVAENIHARNILAYSIVPARVDPAVQAAARDLAVRIAEGLGLVGLLGVEMFCLPDGRLLVNELAPRPHNSGHYTQDACATSQFEQHIRAVCDLPLGAVDLLSPVCMVNVMGEDFPLDVGAALSDPHVKLHLYGKREPRPHRKMGHLNVLAPTADEALERAREAKAAIAGTAGQPRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH4</accession>
    <name>Q67NH4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1784</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195912.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40769">
        <property type="protein sequence ID" value="BAD40769"/>
        <property type="gene ID" value="STH1784"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1784"/>
    <dbReference type="HOGENOM" id="CLU_1721432_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="16402" checksum="D5D88938D6EC867C" modified="2004-10-11" version="1">MKLGMELVARHPLPLSLGTFFETWIATAAGSARRSLGRDEYHLRIHVDTGARLAATGRTHVCQVDLEAAGLGRNGARPALLTPVDVPDGSPVIWGIRTNRFLDVADLIASAGARLLREGSEPAPFALDDPRVRLECVAPGRYIVDITRLLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ1</accession>
    <name>Q67NJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1767"/>
    <dbReference type="EnsemblBacteria" id="BAD40752">
        <property type="protein sequence ID" value="BAD40752"/>
        <property type="gene ID" value="STH1767"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1767"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_9"/>
    <dbReference type="OMA" id="EERSIDC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="34672" checksum="9884587DC2D40AB0" modified="2004-10-11" version="1">MRGPTRRGSSMLLQHLTTFCRVVEEGSFTRAAQVLNLTQPSVTKQVGALEDYLQVQLFTRQGKRVHLTPVGELVYDYARQVIHLVQRCEEAVREYRSPGSGSVTVGCVHTIGLFTLPELLAAYVREHPRVKINVKTGNNRETVTMLLHGEVDVGLVTTPQVHERIEVVPLFEDPLVVVAGPSFAADLPPVVSNADLAQLPFIGYVRGARFRMTTDQVLEEMGIQPQFVMEFDNHEAIKTMVALGFGIALEPVSAVQRELISGQLVRLNVPGLPRLSRTTSLILRRGERRSEAVRAFLSLLEQRYNVTIPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK9</accession>
    <name>Q67NK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195877.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1749"/>
    <dbReference type="EnsemblBacteria" id="BAD40734">
        <property type="protein sequence ID" value="BAD40734"/>
        <property type="gene ID" value="STH1749"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1749"/>
    <dbReference type="HOGENOM" id="CLU_1694628_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="155" mass="18046" checksum="546255C5642C0B10" modified="2004-10-11" version="1">MFTWWQWLLIILAALGLGFLILWQTPWFKVRKYAIDVKRIYDKHKLQEKAEQAQALGQNPNVNPVLLRKPLRDIISTYEAIIADMNKLKVPPKAQKLHEMSLTAAKESLAMYQMAAVGGFRQKAILSRQKKVMQLQEQIEAEMEKLYGKPKQPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN2</accession>
    <name>Q67NN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40711">
        <property type="protein sequence ID" value="BAD40711"/>
        <property type="gene ID" value="STH1726"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1726"/>
    <dbReference type="HOGENOM" id="CLU_2107753_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="12716" checksum="2B5F04E4D94BFD8B" modified="2004-10-11" version="1">MAGVSDEVKRLLGDTVQAGQRILIDPSEDELRAAAERLRKEGKLYCTRCRKQIQDDRFITRRISFGPRLQAVVNLHPECDDQESAQPRERAAGSPAGARPEDGGGDVWARTAGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN4</accession>
    <name>Q67NN4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195852.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1724"/>
    <dbReference type="EnsemblBacteria" id="BAD40709">
        <property type="protein sequence ID" value="BAD40709"/>
        <property type="gene ID" value="STH1724"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1724"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046685_6_0_9"/>
    <dbReference type="OMA" id="VELFWGF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="398" mass="42340" checksum="0172572916CBAB00" modified="2004-10-11" version="1">MLAFGFATVFTLLPVYYINEAELTPFQLVLVGTVLESTVFIFEVPTGVVADVYSRRLSVIIGTLLLGIGLAIEGAIPAVVAIFIGEVIRGIGHTFTSGAMEAWLASEIGAETVGNVYLRGNQVEQGASLLGIATSVALARWGLHAPILAGALIILAAGIFLILAMRETSFVPAARMERGSWKATLDTAREGIRTIRRSPLLVTFVLIALFVGMSGEGYDRLWEAHLLENIPFPSLFDLRATTWFGIIAGLSSVLSIVVTETVHRRLDQSHERSVALFLVLCTAAQAAAVLGFAWSGQFFLAVACHLVASLCKGIAAPLRNSWLVRSVDPQVRATVISTVGQADAIGQTAFGPVIGWIGSVRGLRIALSVAGIALSPLVPLFIRAARVARHKPATAAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ0</accession>
    <name>Q67NZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1618</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195747.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1618"/>
    <dbReference type="EnsemblBacteria" id="BAD40603">
        <property type="protein sequence ID" value="BAD40603"/>
        <property type="gene ID" value="STH1618"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1618"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="EKVYNYF"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030660">
        <property type="entry name" value="ABC_branched_ATPase_LivF/BraG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039137">
        <property type="entry name" value="ABC_branched_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="239" mass="26068" checksum="F313FCFA1915F06E" modified="2004-10-11" version="1">MLEVRDLRVAYGNIEALHGISLTVAEGEIVTLIGANGAGKSTTLRAVSGLVRARSGQILFEGHDITRIPAHRIVALGIGHVPEGRKIFTDMTVQENLELGAYIADPRRFREDLEAVFERFPRLRERRRQSAATLSGGEQQMLALGRALMLRPRLLLLDEPSMGLSPRLVEEIFAIIVALNRAGTTVLLVEQNAAMALSIAHRAYVLETGRVVLEGPAKELRNHPAVKEAYLGGGGSVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P43</accession>
    <name>Q67P43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4-hydroxybenzoate octaprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1565"/>
    <dbReference type="EnsemblBacteria" id="BAD40550">
        <property type="protein sequence ID" value="BAD40550"/>
        <property type="gene ID" value="STH1565"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1565"/>
    <dbReference type="eggNOG" id="COG0382">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034879_5_1_9"/>
    <dbReference type="OMA" id="MVVYPYG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006371">
        <property type="entry name" value="HB_polyprenyl-transferase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PTHR11048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01475">
        <property type="entry name" value="ubiA_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="34713" checksum="D677D2B9FFEEE897" modified="2004-10-11" version="1">MLDPREPGCLFGEGAADAVITVLKRVKTFAELVKCEHTVLNLPFAYLGAFLAAEGWPTWRELFWITVAMVGARTAAMGMNRLFDEEIDRRTPRTATRHLPAGKIRRGEVWAYVGVALVLLLLAAVNLHPLAVKLFPLAVLTFTIYPFTKRFTWLCHVWLGLSVSWGAFGAFVAVRGSVGTEILILVGLITLWNAGFDIIYGTQDLEADRINGVHSIPARFGLAAALRIARALHLGVVLLAGALGIAAGLLPASPWPVTAWPTASWLYLAAWVLVAGLLHYEHSIISPADMSRVDTAFFNVNGYLSVAFSVLTIAALVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P48</accession>
    <name>Q67P48_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fur family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195689.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1560"/>
    <dbReference type="EnsemblBacteria" id="BAD40545">
        <property type="protein sequence ID" value="BAD40545"/>
        <property type="gene ID" value="STH1560"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1560"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_3_1_9"/>
    <dbReference type="OMA" id="HDHVILT"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="17262" checksum="967379EE4C0303CB" modified="2004-10-11" version="1">MALMLKDVYTRLLERGHKLTPQRWAIIAIFLMNRGRHLSADDVFAMLRETHPSNGIATVYRTLELLEEIEVLKKVDFGDGRARYEIRDDDSPHHHHLVCQKCGKITEFEDDLLIALEEAIHRKTGFQITEHVAKFYGVCAECQQAAESASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P56</accession>
    <name>Q67P56_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195681.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1552"/>
    <dbReference type="EnsemblBacteria" id="BAD40537">
        <property type="protein sequence ID" value="BAD40537"/>
        <property type="gene ID" value="STH1552"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1552"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1097488_0_0_9"/>
    <dbReference type="OMA" id="CPTWITY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="34551" checksum="8ED2489C8673E5D9" modified="2004-10-11" version="1">MAQIRDYRPEDAPTLQDLLRQIWGQDTWTVQYYRFGASAPTAQGGFLRTLVADEGGTLVGFGSAWTNPFHPHALYVGINVHPRFGRRGLGTRLLMALAQRSPGRLPLQGSTWEHSESGMRFARRHGFTEVRRTWEPELPLGDPQEDLHADCESRCAALGYAIVPLADLQAASDGLRQLAPLLAEVYTATHGPNPPRTMDADGWTDLLRRDPPDPEASFIALRHGRPVAMSAAHPDPDAGLILSWRGVAAGHRAHERDLILALTRRQVLAAHRRGLPALKGEFDSTDPWAMIQMEAFPFRPAPCWVTFRRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P72</accession>
    <name>Q67P72_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheW3</name>
        <name evidence="2" type="ordered locus">STH1536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1536"/>
    <dbReference type="EnsemblBacteria" id="BAD40521">
        <property type="protein sequence ID" value="BAD40521"/>
        <property type="gene ID" value="STH1536"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1536"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_1_9"/>
    <dbReference type="OMA" id="ERTIDTC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17519" checksum="7B089281D4C9C22F" modified="2004-10-11" version="1">MVQEQREQQSIFQIVVFQMDNEYYGADIAVVREVVPLQRVTRVPRTPAYVLGVINLRGRVIPVIDLRRRLRLCTSAATKATRIAIAEVDGDQVGMVVDSVEEVARVPADAVEPPSSLLSRVDREHVLGVAKVGGRLVTLLDLRQILVREERKTPVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P76</accession>
    <name>Q67P76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Processing protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1532</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195662.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1532"/>
    <dbReference type="MEROPS" id="M16.A15"/>
    <dbReference type="EnsemblBacteria" id="BAD40517">
        <property type="protein sequence ID" value="BAD40517"/>
        <property type="gene ID" value="STH1532"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1532"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_3_0_9"/>
    <dbReference type="OMA" id="IDVVCDM"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="3">
        <location>
            <begin position="14"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="4">
        <location>
            <begin position="168"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="426" mass="46669" checksum="C725D23060A113CB" modified="2004-10-11" version="1">MTFYRKTTLPNGLRVVTEAIGHVRSAAVGVYVGTGSLYEAPAEMGVSHLIEHMLFKGTERRSALEIARAIDGRGGALNAYTAKEYTCYYARVLDEHLPLALDVLADMILNSRFDPDDLAREKDVICEEIRMYDDVPDDLVHDLFAGALWRGHALGRPIVGTVERVQAMSRADILAYKNRHYVPANMVVAAAGHLEHERVVEWVAELFGAAAAEADGRPAPDAPPVPRTPAIAVRQKEIEQAHLVLGTTALSLDDPNIYALHVLNAIVGGSSSSRLFQEVREKRGLAYSVYSYHSSYRSAGAFGVYAGVSPRMVGATLDVVTGVLSELGRRGVTEEELAEAREQLKGQLMLGLESTSSRMSRLGRGELIRGFVHSPDEVIARVEAVTLEQVNELAHRLFVEEARVMAAVVPHALQYDFAQYGEVLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P82</accession>
    <name>Q67P82_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="11">Riboflavin kinase</fullName>
                <ecNumber evidence="11">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">FMN adenylyltransferase</fullName>
                <ecNumber evidence="11">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="11">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH1526</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="11"/>
    <dbReference type="EC" id="2.7.7.2" evidence="11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195656.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1526"/>
    <dbReference type="EnsemblBacteria" id="BAD40511">
        <property type="protein sequence ID" value="BAD40511"/>
        <property type="gene ID" value="STH1526"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1526"/>
    <dbReference type="eggNOG" id="COG0196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_0_0_9"/>
    <dbReference type="OMA" id="HRGHQAI"/>
    <dbReference type="OrthoDB" id="1504821at2"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11" id="KW-0288">FMN</keyword>
    <keyword evidence="11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="12">
        <location>
            <begin position="183"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="35973" checksum="7CC1ADCCACE6329C" modified="2004-10-11" version="1">MELVTRIADVPVRGTGNLIAIGKWDGVHLAHQAILRALVAEARRVGGQAVAVGFHPLPMQVLRPEAAPPMLQSLEERAELMAALGVDVHLALPFDRALADMTPEAFVHQVLVGQLRAQQVMVGFNNTFGRGGKGTAETMRQLCTPLGIPVHIFQPVRADGENVSSTEVRFAVAKGRMQAAARLLGRPYAVRGLVVQGDRRGRELGYPTANIRLEEGRLLPPHGVYTARVTVLGEPRKLQPPVTVTPRTGPVYGAMLNLGVRPTVGGTELRCEAHLFDFRGDLYGRELQVEFLEYMRPERAFPNLDALVAQLRADERAAREFLAGRQGAEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB7</accession>
    <name>RS2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsB</name>
        <name type="ordered locus">STH1491</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195621.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PB7"/>
    <dbReference type="SMR" id="Q67PB7"/>
    <dbReference type="STRING" id="292459.STH1491"/>
    <dbReference type="EnsemblBacteria" id="BAD40476">
        <property type="protein sequence ID" value="BAD40476"/>
        <property type="gene ID" value="STH1491"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1491"/>
    <dbReference type="eggNOG" id="COG0052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040318_1_2_9"/>
    <dbReference type="OMA" id="SYIDRAY"/>
    <dbReference type="OrthoDB" id="1623045at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000134257" description="30S ribosomal protein S2">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="264" mass="29922" checksum="8D2FBC2904E5A7B6" modified="2004-10-11" version="1">MSVISMKQLLEAGVHFGHQTRRWNPKMKEYIFTERNGIYIIDLQKTVRMVEIAYNAVRQMAADGGKILFIGTKKQAQDAVREEAERCGMFYVNQRWLGGMLTNFETIKKRIARLNELEEIEGTEYWSKLTKKEQAQLLHEKEKLEKNLGGIKGMKSLPDMAFVIDPRRERIAVSELRKLGIPIVAIVDTNCDPDEIDYVIPGNDDAIRAVKLLTSKIADAVIEGRQGVDTSATVDEEEAEVAEETESMESAEDLDADLIEEEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD2</accession>
    <name>Q67PD2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family. ComM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1476"/>
    <dbReference type="EnsemblBacteria" id="BAD40461">
        <property type="protein sequence ID" value="BAD40461"/>
        <property type="gene ID" value="STH1476"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1476"/>
    <dbReference type="eggNOG" id="COG0606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026145_1_1_9"/>
    <dbReference type="OMA" id="FNGAATY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004482">
        <property type="entry name" value="Mg_chelat-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025158">
        <property type="entry name" value="Mg_chelat-rel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF7">
        <property type="entry name" value="PTHR32039:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13335">
        <property type="entry name" value="Mg_chelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00368">
        <property type="entry name" value="TIGR00368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="2">
        <location>
            <begin position="287"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="510" mass="53997" checksum="5ED710B836171C8C" modified="2004-10-11" version="1">MRRVFVRVWSFTLQGIEALPVAVEVDVSPGLPSFEIVGLPDAAVREARERVRAAVRNGGWPFPLQRITVNLAPAHTRKEGAGFDLAIALGVLAAAGAFHPSALAGIAVAGELALDGGVRPVRGALAMAMALEPGRRLVLPPESAREAAACGADVLAAPHLADLVAHLRGERVLAAPDPAPPVAGGCDGDVDLALVRGQPVARRALEVAAAGGHNLYMVGPPGAGKSLLARCLPTILPPLDEAEALEVSRIHSVAGELGPGGLLRRRPFRAPHHSISRAAMLGGGNPLRPGEVTLAHRGVLFLDEMPEFRRDVLEGLRQPLEDGVVRVARAHAHLTFPARPTLVAAANPCPCGHLGDPVHACTCTATAVHLYRNRLSGPLRDRFDLQVYLQPVPYAAYRAAAAGESSRAVAERVREARERQRRRLAQHGCSSNAEMGPALIRRFCRIPPGAEALLREAVDRFGLSVRGCDRVLRVARTLADLEGADTVELRHLAEALQYRGVGAAPVRQGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG9</accession>
    <name>COAD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="1">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaD</name>
        <name type="ordered locus">STH1439</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195569.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PG9"/>
    <dbReference type="SMR" id="Q67PG9"/>
    <dbReference type="STRING" id="292459.STH1439"/>
    <dbReference type="EnsemblBacteria" id="BAD40424">
        <property type="protein sequence ID" value="BAD40424"/>
        <property type="gene ID" value="STH1439"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1439"/>
    <dbReference type="eggNOG" id="COG0669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100149_0_1_9"/>
    <dbReference type="OMA" id="EFQMALM"/>
    <dbReference type="OrthoDB" id="1846503at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156290" description="Phosphopantetheine adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <sequence length="162" mass="18329" checksum="CBAB6D6D60D0ADCC" modified="2004-10-11" version="1">MIKAVCPGSFDPVTLGHLDIIERAARTFDEVVVAVLTNPRKEPLFTVEERLEMLREATKHIPNVSVAAADGLLVDFARQQGCRVIVKGLRPIQDFEYEWQMGAVNRQLDGNIETCFLMSRIEYAHLSSSIVRELAYFGRPTEGLVPPFTARRLREKFAKTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN0</accession>
    <name>Q67PN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195508.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1378"/>
    <dbReference type="EnsemblBacteria" id="BAD40363">
        <property type="protein sequence ID" value="BAD40363"/>
        <property type="gene ID" value="STH1378"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1378"/>
    <dbReference type="HOGENOM" id="CLU_1288346_0_0_9"/>
    <dbReference type="OrthoDB" id="1713509at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="23072" checksum="75E29F6457168EA2" modified="2004-10-11" version="1">MPEERLQPAQLEPICIRTTQVYDWCYLNGTTSVLLPDIVFPADTSVVAGIEAAIISVECAEIDRDQDPSGLANVTIRRLVTFELTFVDVNGNPVPVIVNGVEVTTQTRSRFFDEFIRVCAPDGTTLTCEITDAAARAFMTVINGTPAVQVELFICQNIQVTADVTVSININDYCLPDACQPGQKPFQCPPPTTPPPACGPLTQGRARKAGSSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN1</accession>
    <name>Q67PN1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heavy metal-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1377"/>
    <dbReference type="EnsemblBacteria" id="BAD40362">
        <property type="protein sequence ID" value="BAD40362"/>
        <property type="gene ID" value="STH1377"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1377"/>
    <dbReference type="eggNOG" id="COG2608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2588424_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="12"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="80" mass="8721" checksum="B99CC479B7B723C3" modified="2004-10-11" version="1">MSAQSVAENLVATSLKVYGMRDAAAAQRAYDALRQIDGVQQISMNVPLRRISVTHTPRRGITQELVAALRSLGFHAQEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ1</accession>
    <name>Q67PQ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1357"/>
    <dbReference type="EnsemblBacteria" id="BAD40342">
        <property type="protein sequence ID" value="BAD40342"/>
        <property type="gene ID" value="STH1357"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1357"/>
    <dbReference type="HOGENOM" id="CLU_3030777_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="55" mass="5954" checksum="B0672DF13FD009DB" modified="2004-10-11" version="1">MRVRLGRRRPPRRTLVGFVLILAGVSIVLTALPGFVYAAVIGGLIAYIGYTLIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT2</accession>
    <name>Q67PT2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1326</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1326"/>
    <dbReference type="EnsemblBacteria" id="BAD40311">
        <property type="protein sequence ID" value="BAD40311"/>
        <property type="gene ID" value="STH1326"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1326"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_3_0_9"/>
    <dbReference type="OMA" id="REYMADS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="84"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="301" mass="31790" checksum="38D03FC62A040049" modified="2004-10-11" version="1">MTFPDLCRTIKGKGGGGLMLTFRTTVCLVALFLLAALVGLTTVGPFGAAVVIAVSLLTSTAAYRGRRWASLRQMGGRPIQWFEAPDLYELVDALARRAGLPTPRLYLLPGRMVNAVAVATAGSSAIGVTAPLLRYMPPDEVAAVIAHEIAHIRHGDLPLQMVAAGLAGAATALAEIGRFGVVFAWLLGFPVGLGELAAALLIAAGVPAVALVLRMAISREREYLADAGAAELVGSPQRMARALGRLERLNQPAWWQRLLGFPAPQEPTGWAALLSSHPPTRLRIARLLAMSPPRPDLACFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q17</accession>
    <name>Q67Q17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DksA C4-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195372.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1241"/>
    <dbReference type="EnsemblBacteria" id="BAD40226">
        <property type="protein sequence ID" value="BAD40226"/>
        <property type="gene ID" value="STH1241"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1241"/>
    <dbReference type="eggNOG" id="COG1734">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043144_1_0_9"/>
    <dbReference type="OrthoDB" id="1932026at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037187">
        <property type="entry name" value="DnaK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014240">
        <property type="entry name" value="YteA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020458">
        <property type="entry name" value="Znf_DskA_TraR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01258">
        <property type="entry name" value="zf-dskA_traR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109635">
        <property type="entry name" value="SSF109635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02890">
        <property type="entry name" value="bacill_yteA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01102">
        <property type="entry name" value="ZF_DKSA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="6">
        <location>
            <begin position="46"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="4">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="30963" checksum="4A1099D20AD38158" modified="2004-10-11" version="1">MQIEELRQMLLQEREAYQRQVAMIEETGLGTSQGDQLQEDSTVDNHPADLGTETFERSKDLGLRSHMLRRIQEIESALQRMDQGTYGICEECGRPIDPERLKAFPSATTCITCQQRREARPDRFVRPIEELVTGPPFSRSFRDRSGDPGYDGEDAWQEVGQYGTSETPQDVPGAVSYEDMYNSDEDVYTIVDPMDALVDEDGEPLQRDDRDWDGGTVQWTEGEEGTLYGPEDMGGEYRWDPGPYFERVMPERQFRRGKFPRRRMGRPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q18</accession>
    <name>Q67Q18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1240"/>
    <dbReference type="EnsemblBacteria" id="BAD40225">
        <property type="protein sequence ID" value="BAD40225"/>
        <property type="gene ID" value="STH1240"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1240"/>
    <dbReference type="eggNOG" id="ENOG5032Z2B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155870_0_0_9"/>
    <dbReference type="OMA" id="LLWVNFI"/>
    <dbReference type="OrthoDB" id="2081708at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043723">
        <property type="entry name" value="DUF5665"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18910">
        <property type="entry name" value="DUF5665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12721" checksum="660C6395BF70A762" modified="2004-10-11" version="1">MPRRWRVKRRAEKGAAGPAHTAAGESLAQMVERLSIALERSNVLDYTVLLQHPWRMLWINFVGGMARGLGIGIGFTVLSAILLYILRGLMMANLPFIGDLIATIVRLVEQQLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF5</accession>
    <name>Q67QF5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative polysaccharide pyruvyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1103"/>
    <dbReference type="EnsemblBacteria" id="BAD40088">
        <property type="protein sequence ID" value="BAD40088"/>
        <property type="gene ID" value="STH1103"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1103"/>
    <dbReference type="eggNOG" id="COG2327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039510_0_1_9"/>
    <dbReference type="OMA" id="TVAWAQG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019896">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase_CsaB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03609">
        <property type="entry name" value="S_layer_CsaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PS_pyruv_trans" evidence="1">
        <location>
            <begin position="14"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="365" mass="38820" checksum="70FF561F109084F0" modified="2004-10-11" version="1">MAEILIAGYYGFHNAGDEAMLAGLRRAIRELRPDVTFTAISGTASQTRRLHGIGAVSRSDIRGIWEAIGRADLVMFGGGSLLQDVTSSRSLIYYLGLATMARLRRKPVMFCAQGIGPVTRPLGRALVPLIVNRAALITARDAEAAETFRRLGVTRPPVTVTADAALVLGPADPAAGEALLRGAGVDVSRPVIGVSVRPWKPGREPLEPRLAEALDRLAGETGAQVVFIPMQQVKDVEASRAVVACMRAPAVVLNGPYTYEDLRAVIARCDLLIGMRYHSLVFAAMSGVPLVGISYDPKNDAFLRQIGHEAAGSTGGLNPAAVAAAGRRALADAEKVRADLLRQMDRLIPLARLNAELAVGLLPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF9</accession>
    <name>Q67QF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195231.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1099"/>
    <dbReference type="EnsemblBacteria" id="BAD40084">
        <property type="protein sequence ID" value="BAD40084"/>
        <property type="gene ID" value="STH1099"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1099"/>
    <dbReference type="eggNOG" id="ENOG50323RE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133815_0_0_9"/>
    <dbReference type="OrthoDB" id="1716054at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025480">
        <property type="entry name" value="DUF4330"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14221">
        <property type="entry name" value="DUF4330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="17970" checksum="3044944E05B19FAB" modified="2004-10-11" version="1">MLIDNKGRLFGKINLLDLVVLLGILAVAGRFVYGALTGPAATPTGQDQVIEMTLRIPAVTQWTIDAIQVGDEVYDSKSNTRMGQIVDAWWEPAVVVREMPDGIVPHESDTHFDLYVTVRGPARVSPNGVTMSGIEVKVGRSNQYKSAFWAATGTTVAFDLNPPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN4</accession>
    <name>Q67QN4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195156.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1024"/>
    <dbReference type="EnsemblBacteria" id="BAD40009">
        <property type="protein sequence ID" value="BAD40009"/>
        <property type="gene ID" value="STH1024"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1024"/>
    <dbReference type="HOGENOM" id="CLU_1199304_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="24530" checksum="97A027CE5675D6E8" modified="2004-10-11" version="1">MTVQATTPATSERSGRIPRAVPVLAFLCAALVGLWWRLPLLVAPVADFEQGPGGTEAYVTVATQIREQSASFWTGGDVHLRLSEAEVSGMLSSALLSGGSPDGPLARVRGGVDDGVLLVEAVVAPPADRVPARLAGPVGLRLRLAPEVDETGVIQFRITGAHVGKIPVSPHLIRLAGWLLQPRWQGYDAREAAFILPVSDMISQALGRRIEIRSFTAGQGQLSLTIAMPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW2</accession>
    <name>Q67QW2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hsp20 family heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH946</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH946"/>
    <dbReference type="EnsemblBacteria" id="BAD39931">
        <property type="protein sequence ID" value="BAD39931"/>
        <property type="gene ID" value="STH946"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH946"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="32"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="141" mass="15725" checksum="C7BA8A8CFC50FD36" modified="2004-10-11" version="1">MYYPMRQWNPFDLFDRMLSQWQHVLGSFWPAVPSLPSLDQMDVQQGDQDAVIRIEVPGISPEDLSVTVDGNMLTIRAVHRAIPMGGEEGGAQGVATYQRSFTLPPSVDADKITARYHHGVLEIRLPRTQGQGGRQIPIDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW6</accession>
    <name>Q67QW6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195074.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH942"/>
    <dbReference type="EnsemblBacteria" id="BAD39927">
        <property type="protein sequence ID" value="BAD39927"/>
        <property type="gene ID" value="STH942"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH942"/>
    <dbReference type="eggNOG" id="COG1708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082941_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025184">
        <property type="entry name" value="DUF4111"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13427">
        <property type="entry name" value="DUF4111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF4111" evidence="1">
        <location>
            <begin position="142"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="27587" checksum="76BAB3FA2590FF18" modified="2004-10-11" version="1">MDMSLPPDVAELMQSFASGLRRVLGEKLVGVYLGGSVSLGDYCAGSSDLDFLVVTDGRLSPADLDALAVFHREFLTAHPSAGRLEGDYAPRECIIPEGTTVPVPRCKRGAFVPEVDQVMLSADNICNMRENGIAFYGPPPAEVLPPVSPDQVRAAVRAMLAEAPKPAQRPEEQADELLDLLRSLCALETGKPTTKAQGAEWARRHLEPRWHPVVDAALAVRRGGPAAGWDEGMARSAAELDRLLRERYAIAAQPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R06</accession>
    <name>Q67R06_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH902</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH902"/>
    <dbReference type="EnsemblBacteria" id="BAD39887">
        <property type="protein sequence ID" value="BAD39887"/>
        <property type="gene ID" value="STH902"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH902"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_5_1_9"/>
    <dbReference type="OMA" id="WRVGIQH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="34781" checksum="481F1313CA98A3C5" modified="2004-10-11" version="1">MALLIAAAFFAALWHGGRTERVERTAIMMDTSVRVVVYAGGWDAESAADAAMDSIARLESLWHPGRADSDVSRINAAAGAKPVVVAPETVELVQAALQVARESRGAFDPTIGPLAAAWGFGEGGRVPDAAELEAARALVSWRDVHVDPEAGTVFLSRPGMQLELGAVAKGFAARLVRDQLAQAGVRAALVQLGGSIALLGDRPEGGPWRIAVQHPRDPQGYIATLSLDGGYADTAGDYQRYFEADGVRYHHILDPSTGYPARGMASVTVVAPRGELADAYATAAFVLGMEGGYRFLVERGVEGLLVSDAGEVRMTPGMAELAELTQDAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R09</accession>
    <name>Q67R09_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion-translocating oxidoreductase complex subunit E</fullName>
            <ecNumber evidence="7">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rnf electron transport complex subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnfE</name>
        <name evidence="9" type="ordered locus">STH899</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195031.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH899"/>
    <dbReference type="EnsemblBacteria" id="BAD39884">
        <property type="protein sequence ID" value="BAD39884"/>
        <property type="gene ID" value="STH899"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH899"/>
    <dbReference type="eggNOG" id="COG4660">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046659_1_1_9"/>
    <dbReference type="OMA" id="DGFMMGL"/>
    <dbReference type="OrthoDB" id="1782047at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00478">
        <property type="entry name" value="RsxE_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010968">
        <property type="entry name" value="RnfE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01948">
        <property type="entry name" value="rnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="24502" checksum="AFBDB8F388456B5F" modified="2004-10-11" version="1">MKPSAQSPTFGSVLADGLRQAPPFRLLLGLCPSLAVTTAMENGFWMGLAVIFVLSCSELIISLLRRVIPDRVRIPVYIVIVATFVTVVDMVMKAYLPEMHAILGIFIPLIVVNCIILGRAEAFASKNGPLLSVADAVGVGLGFTASLMAIGGVRELLGTGNLKLFGVALLGPGLPLEVVVMLLPAGAFFVIGFLMAGLAWLQQRQEARGRRDRPASGSGSTAGAGTVSGGEEAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R41</accession>
    <name>Q67R41_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavin_Reduct domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194999.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39852">
        <property type="protein sequence ID" value="BAD39852"/>
        <property type="gene ID" value="STH867"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH867"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_4_2_9"/>
    <dbReference type="OMA" id="CANTCFQ"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="10"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="16939" checksum="90765EEE592F9BC1" modified="2004-10-11" version="1">MDAQVKKQVLRKLTYGLYVATAKQGDQAAAATVNWLSQASFQPPLVMMAVKADSGLHALLSQGATVAVNILGADQKGMAETFFRPTQLEGNTLNGVPFTPGETGAPLLQGVPAAFEAKVVHSYAGGDHTVFVMEVVAVHLYDPEAKVLEMWDTGWFYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R42</accession>
    <name>Q67R42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative starvation induced DNA binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Dps family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH866"/>
    <dbReference type="EnsemblBacteria" id="BAD39851">
        <property type="protein sequence ID" value="BAD39851"/>
        <property type="gene ID" value="STH866"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH866"/>
    <dbReference type="eggNOG" id="COG0783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098183_0_0_9"/>
    <dbReference type="OMA" id="KHHWLTE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016722">
        <property type="term" value="F:oxidoreductase activity, acting on metal ions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002177">
        <property type="entry name" value="DPS_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023188">
        <property type="entry name" value="DPS_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932">
        <property type="entry name" value="PTHR42932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005900">
        <property type="entry name" value="Dps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00819">
        <property type="entry name" value="DPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin" evidence="2">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="188" mass="21698" checksum="E158F5B6FF9A1B19" modified="2004-10-11" version="1">MGAGIGLPYEVRLEMALQLDDHQCALNVALHQYRKHHWLTEGAEAFHSLHHLLDEHIEKTSKHIDDIGERVARLGGVPTAHPVTQHELSYIKHEVEGRHSMRDFLRNDLEHELKIQEMLRKTIKRAHELGDWGTVEVLEDILVEREDLAYHLYSVLEDDTLVRGMTHLLDGNYDMAGDRPMPPDSKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB9</accession>
    <name>Q67RB9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mrr_cat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194922.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH789"/>
    <dbReference type="EnsemblBacteria" id="BAD39774">
        <property type="protein sequence ID" value="BAD39774"/>
        <property type="gene ID" value="STH789"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH789"/>
    <dbReference type="eggNOG" id="COG1787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2319085_0_0_9"/>
    <dbReference type="OrthoDB" id="1999861at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mrr_cat" evidence="1">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="11302" checksum="70153C071ABA2433" modified="2004-10-11" version="1">MTPPSGDYGVDLILTRPGSPERIAVQAKRYHRKHTVGVRAIQEVYAAKDYYGCSRAIVVTTSYFTENAKQSARKLGVFIIDRDQLAEELRHQQKNQHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD5</accession>
    <name>Q67RD5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribose ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH773</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194906.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH773"/>
    <dbReference type="EnsemblBacteria" id="BAD39758">
        <property type="protein sequence ID" value="BAD39758"/>
        <property type="gene ID" value="STH773"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH773"/>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="46096" checksum="4B68B4EE18A19D9E" modified="2004-10-11" version="1">MPAAPVVEMRGIDRSFPGVRALKGVDFELRPGEVHGLLGENGAGKSTLMKILGGLYRPEAGEVLIDGRPVTIDSPAAATALGIAFIHQELNQALHLSVAENIYLGRPPVTGPFRRVDWRAMYQGAAAVLERLGAQIDPRATLGSLGVGARQMVEIARALSLDARVLIMDEPTAALTEPEVERLFAVMRSLVEAGVAIVYISHRLEEIFTICDRVTVLRDGQRIGSWPIDQVTADHLITHMVGRQLTERFPKVEVAPGDTLLEVRDLLLRGSSTPVSFTVRRGEILGIAGLMGAGRTHRGQGFHEAVDGVQGIDVVASQPADFDRAKGLTVMENILQAHPDIVAVFAHNDEMALGALEAIEAAGKADQIKVVGFDATDDAVKAVQEGRMAATVAQKPKEMGRLGVETALKHLNGEKVDEYIPVPLELVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI3</accession>
    <name>Q67RI3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH725</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH725"/>
    <dbReference type="EnsemblBacteria" id="BAD39710">
        <property type="protein sequence ID" value="BAD39710"/>
        <property type="gene ID" value="STH725"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH725"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_9"/>
    <dbReference type="OMA" id="CPIETPA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="4"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="268"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="393" mass="41016" checksum="7CEAB0AD36BC080D" modified="2004-10-11" version="1">MEDVLILGGARTPFGTFGGALRDISAVDLGVAAARAALERTGVPAAAVDEVVVGNVIHTGKDGPYLARHIALYAGVPVETPALTVNRLCGSGLQAAVSAAHTIRAGEASVALVGGVESMSQAPYHLRGRFGAGIGAPELGDALWETLTDSYCGCGMAITAENLAERYGISREEQDRFALLSQQRAAAARERLAEEIVPVELPARRGAPSRLEQDEHPRPDTTYEALARLPARFKEGGTVTAGNASGINDGAAMLVLASERRAGAEGWRPLGRLVSWAVAGVDPRYMGMGPVPASRKALERAGLTLEEMDLVEVNEAFAAQYLAVEKELELDRERTNVNGGAIALGHPVGASGARLLLTLLLELRRRGGRYGLATLCIGGGQGIAAVVEAFPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT0</accession>
    <name>Q67RT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional antiterminator of glycerol uptake operon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH628"/>
    <dbReference type="EnsemblBacteria" id="BAD39613">
        <property type="protein sequence ID" value="BAD39613"/>
        <property type="gene ID" value="STH628"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH628"/>
    <dbReference type="eggNOG" id="COG1954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111516_0_1_9"/>
    <dbReference type="OMA" id="FVDMDFV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006699">
        <property type="entry name" value="GlpP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35787">
        <property type="entry name" value="PTHR35787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04309">
        <property type="entry name" value="G3P_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016897">
        <property type="entry name" value="GlpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="22452" checksum="A09C9CCADBE1034F" modified="2004-10-11" version="1">MAERRRSEGAQRLAEALREHPVIASVRDEQRLDEALRSRCRTVFLLSTSLGRLAEVGEAVAGAGKLLFIHLDFVGGLGRDEEAVEYLARVARPVGLITTRTNLIQTARRLGLTPLQRLFLLDSQSLATGIEAARSSRAEVVEVLPGIIPRAVQAIRSQLPGALIIAGGLVRTPREVGRALQAGASGVSTSSADLWNMEPAAFAVALEQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT5</accession>
    <name>Q67RT5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative iron-sulfur cluster-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH623</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194756.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH623"/>
    <dbReference type="EnsemblBacteria" id="BAD39608">
        <property type="protein sequence ID" value="BAD39608"/>
        <property type="gene ID" value="STH623"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH623"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005304_1_0_9"/>
    <dbReference type="OMA" id="SGCPYCL"/>
    <dbReference type="OrthoDB" id="1951488at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023234">
        <property type="entry name" value="NarG-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036197">
        <property type="entry name" value="NarG-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02665">
        <property type="entry name" value="Nitrate_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103501">
        <property type="entry name" value="SSF103501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="695" mass="77907" checksum="C93CD5EEB4493686" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV5</accession>
    <name>Q67RV5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH603</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH603"/>
    <dbReference type="EnsemblBacteria" id="BAD39588">
        <property type="protein sequence ID" value="BAD39588"/>
        <property type="gene ID" value="STH603"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH603"/>
    <dbReference type="HOGENOM" id="CLU_1757899_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="16435" checksum="9CE51FB902836F2E" modified="2004-10-11" version="1">MQGNHFLAFLVITSRRVRLQGQPSMGGVPVLQRALGLLRFRAPEPAVREPDHHALMKAALARVETAHQMLREARTLEELDVARSALQQAQAEVQHVIRTAKRERGVALRPIAETEQVYRALRERLKGRGSPSDAQEQPGRPDSPAAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW6</accession>
    <name>Q67RW6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ketoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH592"/>
    <dbReference type="EnsemblBacteria" id="BAD39577">
        <property type="protein sequence ID" value="BAD39577"/>
        <property type="gene ID" value="STH592"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH592"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_9"/>
    <dbReference type="OMA" id="NRMMNAN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="241" mass="25067" checksum="1031BB28BBAD6979" modified="2004-10-11" version="1">MIVTGGGTGIGRATALACARRGAKVLITGRRPEPLRETEAAAPGIRGVTADVTVASDARRVVEEAAKLWGRIDVVVNNAGMFASGPFEKVGAETAAQLFATNVFGPINLVREALPWLKESRGAIINVSSTYGHKAAANVAHYAASKAALDHLTRCWALELAPYGIRVNAVAPGPTETPILSRSGLPEDVVASIKEAEKRQIPLGRRGEPDDVAAWIVALAAPGSHWLTGQVIGVDGGLSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY7</accession>
    <name>Q67RY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH571</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH571"/>
    <dbReference type="EnsemblBacteria" id="BAD39556">
        <property type="protein sequence ID" value="BAD39556"/>
        <property type="gene ID" value="STH571"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH571"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_894080_0_0_9"/>
    <dbReference type="OMA" id="WIHYIET"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269346" evidence="6">
        <location>
            <begin position="31"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="102"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="324" mass="34666" checksum="AB818743BFD1AEA2" modified="2004-10-11" version="1">MRVGLLALSAVIVVLVAMLPEGVEVTGEAAAPGTLGEVRTDATRKYRIEPLQDAGAQFSYTWSAAKYGAGVRNYLVGLFEGTLTVERWYGTAQSRLPLLPLLRDTWWLSFQLFAVALAAGIAAGLLLGGLVLGSRGARIFSVGLSILGVSIPDFLLILLGQMLTIWSYRNFGVRLWSVLGVQGGERGWILPLLALSIIPMAYTARLISTALDEAMREDYIRTARAKGVPEVLVVLKHAMRNALPRALNGMPAMLNVTLSSQLVVEVLTNHYGIGSGLMRGLSYGQPAVVASIGLIFCTWFVVMDGLAVTLRALANPLLKGGGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S07</accession>
    <name>Q67S07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH551"/>
    <dbReference type="EnsemblBacteria" id="BAD39536">
        <property type="protein sequence ID" value="BAD39536"/>
        <property type="gene ID" value="STH551"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH551"/>
    <dbReference type="eggNOG" id="ENOG50332GN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1277084_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="23664" checksum="27C3F192E0F3EB01" modified="2004-10-11" version="1">MMVSARGGAPPVPGLAGRPCAPRRAYRAGAGGRMKGRWLVMNQSTRVRTVVTLLLLIPLLLVRPGRPAAAQEADPFAYCAAVGTVDRPDHRWTGPPVPDAVIEGLIRAAGLPEDAPRDPLRRSTFWRCMGGHVYACFVGANLPCQEKADTRRIPRAAMWRFCRANPGADSIPAVVTGRATVYQWRCTGSRPTIVRQVDAPDARGFLKRIWYRISPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S25</accession>
    <name>Q67S25_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Cytidine deaminase</fullName>
            <ecNumber evidence="6 11">3.5.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Cytidine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH533</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 11">This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>2'-deoxycytidine + H(+) + H2O = 2'-deoxyuridine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13433"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15698"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>cytidine + H(+) + H2O = NH4(+) + uridine</text>
            <dbReference type="Rhea" id="RHEA:16069"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.5" evidence="6 11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194667.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH533"/>
    <dbReference type="EnsemblBacteria" id="BAD39518">
        <property type="protein sequence ID" value="BAD39518"/>
        <property type="gene ID" value="STH533"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH533"/>
    <dbReference type="eggNOG" id="COG0295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097262_0_1_9"/>
    <dbReference type="OMA" id="CGACRQS"/>
    <dbReference type="OrthoDB" id="1895660at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047844">
        <property type="term" value="F:deoxycytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006262">
        <property type="entry name" value="Cyt_deam_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01354">
        <property type="entry name" value="cyt_deam_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="137" mass="14586" checksum="C6566DCAEDDCBCCC" modified="2004-10-11" version="1">MTDAELIAAARDARDRAYVPYSRFAVGAAVLAEDGTLYTGCNIENASFGLTNCAERTAIFKMVSEGRRRFKAIAVIADTDGPCSPCGACRQVMSEFGPDARVILTNLKGDTWTGSVRDLLPGAFTSADLPGDEEGRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S56</accession>
    <name>HRCA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hrcA</name>
        <name type="ordered locus">STH502</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HrcA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194636.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S56"/>
    <dbReference type="SMR" id="Q67S56"/>
    <dbReference type="STRING" id="292459.STH502"/>
    <dbReference type="EnsemblBacteria" id="BAD39487">
        <property type="protein sequence ID" value="BAD39487"/>
        <property type="gene ID" value="STH502"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH502"/>
    <dbReference type="eggNOG" id="COG1420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050019_1_0_9"/>
    <dbReference type="OMA" id="DYPGTMG"/>
    <dbReference type="OrthoDB" id="1510750at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023120">
        <property type="entry name" value="WHTH_transcript_rep_HrcA_IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000182546" description="Heat-inducible transcription repressor HrcA">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <sequence length="341" mass="38714" checksum="FB498EB9D2C3DCB0" modified="2004-10-11" version="1">MIDDRKNKVLQAIIEDYVATAEPVGSRTIARKYNLGVSPATIRNEMSDLEELGYLEQPHTSAGRIPSNLGYRYYVDCLMPERPINPAEQELIRSTFQRKIRELDTLVRETARLLSETTHLTAVISGPQFEKAVFKEIRIVPLGNDRALLIYITDSGLVENQVVEVPLQVTMLELQQVAELLSEHLRGRRVETLSRTALQSLHRELSRYGTLLEQALHFLEQKLEPGDRHRLYFGGTSHMLDQPEFRDVQKLRGVLSFLEQEEAVAAVLGLDRLTEGVEIQIGEEIRLRELADCSVVTATYRVGDRIIGKMGVIGPRRMEYPKVVSILNAIASHLSEMNRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S67</accession>
    <name>Q67S67_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194625.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH491"/>
    <dbReference type="EnsemblBacteria" id="BAD39476">
        <property type="protein sequence ID" value="BAD39476"/>
        <property type="gene ID" value="STH491"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH491"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="LIRCMNL"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="241" mass="26645" checksum="6CF1F18FC0ED632E" modified="2004-10-11" version="1">MIQLIKVNKHFGPLHVLVDIDLTVKAGEKLVVIGPSGSGKSTLIRCINLLERPTSGTVIVDGVDVTAPRAPLTKVRQSIGMVFQQFNLYPHKTVLENVTLAPILVKGVPREEAEATGLQFLERVGLKHKAHAYPSQLSGGQQQRVAIARALNMRPKIMLFDEPTSALDPEMVQEVLDVMISLSQDGITMVVVTHEMGFAREVADRVIFMDGGQILEEGPPEHFFESPTHERTKLFLSRILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S71</accession>
    <name>Q67S71_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alkaline proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39472">
        <property type="protein sequence ID" value="BAD39472"/>
        <property type="gene ID" value="STH487"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH487"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_6_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07484">
        <property type="entry name" value="Peptidases_S8_Thermitase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034084">
        <property type="entry name" value="Thermitase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269520" evidence="7">
        <location>
            <begin position="22"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="8">
        <location>
            <begin position="152"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="565" mass="60037" checksum="145C72EEDF53A7A9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S84</accession>
    <name>Q67S84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194608.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39459">
        <property type="protein sequence ID" value="BAD39459"/>
        <property type="gene ID" value="STH474"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH474"/>
    <dbReference type="HOGENOM" id="CLU_492520_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="449"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="490"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="553" mass="57148" checksum="7A1908E571D04196" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA8</accession>
    <name>Q67SA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4-oxalocrotonate tautomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-oxalocrotonate tautomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH450"/>
    <dbReference type="EnsemblBacteria" id="BAD39435">
        <property type="protein sequence ID" value="BAD39435"/>
        <property type="gene ID" value="STH450"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH450"/>
    <dbReference type="eggNOG" id="COG1942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1958482_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Tautomerase" evidence="2">
        <location>
            <begin position="72"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="128" mass="14565" checksum="2033E583E3B3324B" modified="2004-10-11" version="1">MHRERSDRPMPFVTVDHFAGVDPQVRRLLQQRVAQVVVETLGAPPENVRVFTRAFSPEDAYRGDGDAAAALPMIRVELMSGRPLELKRRLMVALARTVAETLEVDVAQIRTVMYENEPYHFCFGETPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB1</accession>
    <name>Q67SB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH447"/>
    <dbReference type="EnsemblBacteria" id="BAD39432">
        <property type="protein sequence ID" value="BAD39432"/>
        <property type="gene ID" value="STH447"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH447"/>
    <dbReference type="HOGENOM" id="CLU_612394_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="447" mass="46182" checksum="D0A10FA5C27FD228" modified="2004-10-11" version="1">MKASLDDQGTGLSPGVIATIDVAEVNDPPSGQPDRWRTQLRDSGPWKIPFSVLTANDLPGPENEGGQSLTVDAVVTDGSDPAHAPVGGTVRIEGADVIFTPDPGFTGEAAFSYYVRDDGATGGQPDPQTSPQPARVTFVVRAPSPPPPPKVTLSAPATVTRESEITVTGTANPGAEVTVNGQTVWADIQGNWSARVTLREGRNLITAVSGTARDSVIVIRDTVPPALSLHAPALRTAEDSVTLTAEAGEGATVWIEGVQGRSLTVSLAVGVNRFTALAEDAAGNRTVAAIEIIRVETAPEPVVVEPGAGAAVGLSQFRGELAGGAAAQPVALAIHSPTLNMEALERVQGAGLIAASGDVEATGMESGEQVHELNAPAMVRFTYDPSVVTVPDRLRIYSFDPAQQVWVELGGEVDPEMSTITVQVERLATFAALVPEAGAPVMDAPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG0</accession>
    <name>Q67SG0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative secretion ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194532.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH398"/>
    <dbReference type="EnsemblBacteria" id="BAD39383">
        <property type="protein sequence ID" value="BAD39383"/>
        <property type="gene ID" value="STH398"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH398"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_4_1_9"/>
    <dbReference type="OMA" id="HDERVIT"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="89"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="432" mass="47409" checksum="C6A5340F38303184" modified="2004-10-11" version="1">MERRQERAGGGAPAKRAHDRFRPVIEAVRERLRGGGAPLGAMVDREARDYLWSLLEEVLAERFPYLNPTHAEKAALVDRLQFQIYGFGILDPLLQDDSVKEIMVNGPDRVFVERDGHPELAVAEDGRPLAFESEADLLHLIERIVARVNRKVDEATPIVDARLPGGARVHVVLPPVSLIGPVLTIRKFPSRPMTLEQMVERGTLTPPAAEFLERLVRARCNLVVSGGTSTGKTTFLNALSMKIPPHERVISIEDSAELQLTGLPNLVSLEARPPNVEGRGALSIRDLVRASLRMRPDRIIVGECRGGEALDMLQAMNTGHDGSLTTVHANSARDALARIETMALMSGVELPLSAIRQQVAAAVDFILHLERLADGSRKLLQIVEVVGVHGGEVIVEPVFRYDLEAGELLPAGRQVARREKFRMAGIPLPEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH0</accession>
    <name>Q67SH0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194522.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH388"/>
    <dbReference type="EnsemblBacteria" id="BAD39373">
        <property type="protein sequence ID" value="BAD39373"/>
        <property type="gene ID" value="STH388"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH388"/>
    <dbReference type="eggNOG" id="COG3745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1000878_0_0_9"/>
    <dbReference type="OMA" id="IAFAHAN"/>
    <dbReference type="OrthoDB" id="2060643at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017592">
        <property type="entry name" value="Pilus_assmbl_Flp-typ_CpaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031571">
        <property type="entry name" value="RcpC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16976">
        <property type="entry name" value="RcpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03177">
        <property type="entry name" value="pilus_cpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="2">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="278" mass="29037" checksum="774B21360996088F" modified="2004-10-11" version="1">MRGSLGRLALVGAFVCALLAAWFNIRFLEMQQERFTVLTVVREVPPYTPVGPDAVQFAAVPRESVPADAITDPSALEGRYTRALLIPGTILREAHLVPAGGSSLAARLAAAASTDVRAMALRVDEATGVAGTLQEGDPVDILVAINADVTEAQERGEGRPARGTLARVIAQRVPVLHVQRDEAGGPSAVVLQVTPRTAEEIAFAHANGMIWLLTSPYTGPDEPVATTGVDREIFFERYGIGQRDTGEAEPAGVPSIAPQGPGEAPAAARPVAGLLGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH9</accession>
    <name>Q67SH9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative allophanate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194513.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH379"/>
    <dbReference type="EnsemblBacteria" id="BAD39364">
        <property type="protein sequence ID" value="BAD39364"/>
        <property type="gene ID" value="STH379"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH379"/>
    <dbReference type="eggNOG" id="COG1984">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028967_5_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010016">
        <property type="entry name" value="KipI_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00370">
        <property type="entry name" value="TIGR00370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS1" evidence="5">
        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="AHS2" evidence="6">
        <location>
            <begin position="264"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="561"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="637" mass="67384" checksum="034E3E3E2D0900DC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK2</accession>
    <name>Q67SK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative pyridine nucleotide-disulphide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194490.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH356"/>
    <dbReference type="EnsemblBacteria" id="BAD39341">
        <property type="protein sequence ID" value="BAD39341"/>
        <property type="gene ID" value="STH356"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH356"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_2_9"/>
    <dbReference type="OMA" id="ITHAPCG"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="469"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="559" mass="59021" checksum="E73BE93B9ED522A5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ0</accession>
    <name>Q67SQ0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH308</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH308"/>
    <dbReference type="EnsemblBacteria" id="BAD39293">
        <property type="protein sequence ID" value="BAD39293"/>
        <property type="gene ID" value="STH308"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH308"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_2_2_9"/>
    <dbReference type="OMA" id="NHTVVMA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="47627" checksum="B17598EA28F567A2" modified="2004-10-11" version="1">MSVYHGKTKKRQMLEVDKQHWLPQQVQVTLHALMGAAKEGLLALAVAVGLDVLRSMMEAEVTAIVGPKGKHNPNRKAFRHGAEEGRVVLGGRKVPIQRPRVRTKDGQEVRLSSYEAFQDEQLLTQAALERMLHGLSTRRYHHGLEPVGDDLPAVATSKSSVSRHFVAATRKALAELLARPLGDQRYLVLMLDGIEVADHTVVVALGITDDGQKQILGLWEGATENATVCRALLTDLVERGLRVDGGILVVIDGAKALRAAVRDVLGARATVQRCQVHKKRNVLDHLPDEARAWVSRKLEQAWRETDYEKARTALTSLAKTLDDKYPGAAASLREGLEETLTVLRLELPEALRKTLRSTNPIESAFEKVRMASRNVKRWRNGQQVLRWTAAGLLEAEKGFRRIKGYRQLPMLSEALSRHAAPEASSAVQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS0</accession>
    <name>Q67SS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH288"/>
    <dbReference type="EnsemblBacteria" id="BAD39273">
        <property type="protein sequence ID" value="BAD39273"/>
        <property type="gene ID" value="STH288"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH288"/>
    <dbReference type="HOGENOM" id="CLU_2235203_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="105" mass="11192" checksum="DEB57466126B7131" modified="2004-10-11" version="1">MAHGGRTNEQSPGRRLLACPGTAQSNRMLRSNSSILANRRMRTRMSGGVRGRGLTAPSYSIEPIRLSAPDTGAGTDRNHHQVTAASRRFRSLPCTALPAAASGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW2</accession>
    <name>Q67SW2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194381.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39231">
        <property type="protein sequence ID" value="BAD39231"/>
        <property type="gene ID" value="STH246"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH246"/>
    <dbReference type="HOGENOM" id="CLU_1365619_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="21198" checksum="F1BA66EE2BBC4362" modified="2004-10-11" version="1">MELLILPLFVLGVAAIVAFWNRAGRWSDDDVGTAVGNALETLNAALGGGEHLAALEYRRQVHDEGEQAGGAGPQAFFPGVRDDVVVIRPPRGADEGGPPRPDEAALTAVVEVSGAAVGRVRFRPLGAGAWRLEELSAPPDVYVRTARLLLAYLFEIEEAGRVELAADVAGLGACGFRDGAVTPETVRPPQHRFSLRLQAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-23" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW4</accession>
    <name>TPIS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Triosephosphate isomerase</fullName>
            <shortName evidence="1">TIM</shortName>
            <shortName evidence="1">TPI</shortName>
            <ecNumber evidence="1">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tpiA</name>
        <name type="ordered locus">STH244</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194379.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SW4"/>
    <dbReference type="SMR" id="Q67SW4"/>
    <dbReference type="STRING" id="292459.STH244"/>
    <dbReference type="EnsemblBacteria" id="BAD39229">
        <property type="protein sequence ID" value="BAD39229"/>
        <property type="gene ID" value="STH244"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH244"/>
    <dbReference type="eggNOG" id="COG0149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_3_9"/>
    <dbReference type="OMA" id="QEVCGAI"/>
    <dbReference type="OrthoDB" id="1266295at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000307583" description="Triosephosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <sequence length="256" mass="26664" checksum="450756BFB8B217E6" modified="2004-10-11" version="1">MRTPIVAANWKMHKTQPEARQFLADFLPREAGMSGVEIVICPPFTALAAVAGGLTGTRVGLGAQNMSDKPSGAFTGEVSGAMLVDAGCRYVILGHSERRRLFGESDEVVGAKVRAALQHGLIPILCIGETLEEQQAGEADAVNRRQLLAGLEGLTPEQVANLVIAYEPVWAIGTGRNCDPGDAQARIAAVRAVVAEAFGPEAAARVRIQYGGSVKPENMAAYMAQPDIDGALVGGASLDPTSFAAICAAAAEARAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T36</accession>
    <name>Q67T36_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH172"/>
    <dbReference type="EnsemblBacteria" id="BAD39157">
        <property type="protein sequence ID" value="BAD39157"/>
        <property type="gene ID" value="STH172"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH172"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_0_3_9"/>
    <dbReference type="OMA" id="WTHADIT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="22"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="22660" checksum="32A9BD32456F3ACC" modified="2004-10-11" version="1">MTWKPSGIGYPLVPSDPSVDRKVAMLTFDDGPSAYTPQVLAGLREAGVKAVFFITGYGAAQHPELVRRIHEEGHLLGIHTMTHPNMTELSREEQIAEIQPLIDLITEVTGQPPKYFRPPYGAYNDQLLATLDELGLELINWSNGSLDWDGVVDGYKDPNQVVADVLDQLHPGAVILAHDTMKHTAEAVPLIIQALRAEGYDFVVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T93</accession>
    <name>Q67T93_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Formate acetyltransferase</fullName>
            <ecNumber evidence="5 14">2.3.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Pyruvate formate-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">STH115</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>acetyl-CoA + formate = CoA + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:11844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Fermentation; pyruvate fermentation; formate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the glycyl radical enzyme (GRE) family. PFL subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.54" evidence="5 14"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194250.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH115"/>
    <dbReference type="EnsemblBacteria" id="BAD39100">
        <property type="protein sequence ID" value="BAD39100"/>
        <property type="gene ID" value="STH115"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH115"/>
    <dbReference type="eggNOG" id="COG1882">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023898_0_0_9"/>
    <dbReference type="OMA" id="PYGGIKM"/>
    <dbReference type="OrthoDB" id="116406at2"/>
    <dbReference type="UniPathway" id="UPA00920">
        <property type="reaction ID" value="UER00891"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008861">
        <property type="term" value="F:formate C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01678">
        <property type="entry name" value="PFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005949">
        <property type="entry name" value="Form_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019777">
        <property type="entry name" value="Form_AcTrfase_GR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001150">
        <property type="entry name" value="Gly_radical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004184">
        <property type="entry name" value="PFL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01228">
        <property type="entry name" value="Gly_radical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02901">
        <property type="entry name" value="PFL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01255">
        <property type="entry name" value="pyr_form_ly_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00850">
        <property type="entry name" value="GLY_RADICAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51149">
        <property type="entry name" value="GLY_RADICAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51554">
        <property type="entry name" value="PFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="14" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="17" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 13 14" id="KW-0556">Organic radical</keyword>
    <keyword evidence="17" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFL" evidence="16">
        <location>
            <begin position="1"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine radical" evidence="15">
        <location>
            <begin position="619"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glycine radical" evidence="13">
        <location>
            <position position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD39100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="742" mass="84353" checksum="1D79B671B80915DE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-11-22" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB8</accession>
    <name>ATPG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpG</name>
        <name type="ordered locus">STH90</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194225.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TB8"/>
    <dbReference type="SMR" id="Q67TB8"/>
    <dbReference type="STRING" id="292459.STH90"/>
    <dbReference type="EnsemblBacteria" id="BAD39075">
        <property type="protein sequence ID" value="BAD39075"/>
        <property type="gene ID" value="STH90"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH90"/>
    <dbReference type="eggNOG" id="COG0224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050669_0_1_9"/>
    <dbReference type="OMA" id="MQITSAM"/>
    <dbReference type="OrthoDB" id="1701531at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="SSF52943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000073401" description="ATP synthase gamma chain">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <sequence length="288" mass="32179" checksum="E646565F99107F95" modified="2004-10-11" version="1">MAENKKAVQRRIRAVKSTQQITKAMKMVDAAKLRRAQEKVQNARPYSRELAKTLGRLIQAGAGIDHPLLHRRTEGTPTVAYVLLTADRGLAGAFNINVIRKAHQALQKESRPTKLITIGRKGRDYFVKRKIQPVLEFVNLGDNITFALARSVLSKIVDLYLSGEVDEVRVIYTEFVNAVTQRPKEMQLLPIQPAQADGAHTHVEYIYEPSPERVLDTLVPRYAETMFYQLMLESKASEHGARMTAMGNATDNAEEMIAKLTLAYNRARQAAITREISEIVGGANALQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH8</accession>
    <name>Q67TH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH30</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH30"/>
    <dbReference type="EnsemblBacteria" id="BAD39015">
        <property type="protein sequence ID" value="BAD39015"/>
        <property type="gene ID" value="STH30"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH30"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_1_0_9"/>
    <dbReference type="OMA" id="LQTFAIM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="18"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="290" mass="30644" checksum="3DA1398D939C4B46" modified="2004-10-11" version="1">MSTKRGDRAMASAPKVGLALGGGFARGMAHIGVLQALVAAGIPIGLVAGTSAGSLVGALYAAGCDPWALEQLAAEMNWRSLVRLRLRRDGLLDTSGLERFLVERVGDLDFRDLRLPFGALATDLLDGQPVLIREGRVTTAVRASCAYPGIFLPVRVGEHTLVDGGLTQPIPARAARSMGADLVIGVELSQDPDPGYRPRNLLDIMMTSLALVQRPLIRASAAAADVLIRPDLREFNAIELDHVGEIVARGRQAAEEMIPAIRERLDALKAVARAGSGPDEPAASREAADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK0</accession>
    <name>Q67TK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Class-V aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH8</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194143.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH8"/>
    <dbReference type="EnsemblBacteria" id="BAD38993">
        <property type="protein sequence ID" value="BAD38993"/>
        <property type="gene ID" value="STH8"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH8"/>
    <dbReference type="eggNOG" id="COG0075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_0_9"/>
    <dbReference type="OMA" id="KNWLPIM"/>
    <dbReference type="OrthoDB" id="996960at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269536" evidence="6">
        <location>
            <begin position="25"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotran_5" evidence="7">
        <location>
            <begin position="12"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD38993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="387" mass="41871" checksum="55807D699A0C73AC" modified="2004-10-11" version="1">MMRMEKSLLMIPGPTPIPPQVAAAMTQPMLNHRGEAFSALFAEVSTKLQKVFRTAQPVYIFPGAGSGGWEAALVNTLNPGDAVLSVSIGDFGERWHKVAVQLGFAVERLRFEPGRAADPERIAERLAEDRERRIKAVLIQHNETSTGVTNDVRAIARAVREHGALLMVDSVSGLGALPLEMDEWGLDVVLTGAQKALMCPPGLSIIAFSERARAAAEKSAMPKFYWDLKAAHSSLQKWQTPYTPAISLLHALKAALELLETETLEGSWARHRLMGEMCRAGVKAMGLRLLAVDERTASNSVTAVVAPVDPKRLRKVAREQFGVELAGGQGDLADQIFRIGHLGYVVPGDVLQALAATEMALAQLGVDVQIGRAVAAAQEVWMNHVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J39</accession>
    <name>Q67J39_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3330"/>
    <dbReference type="EnsemblBacteria" id="BAD42311">
        <property type="protein sequence ID" value="BAD42311"/>
        <property type="gene ID" value="STH3330"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3330"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_12_2_9"/>
    <dbReference type="OMA" id="IRTHARC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="7">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="6">
        <location>
            <begin position="277"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="452" mass="52069" checksum="11E935B334786A23" modified="2004-10-11" version="1">MSKKTYRPYEPNQLLLLPPALQDWLPHDHFAYFLSDVVDSLDLSQIEQTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMSDSRMVEEEQRLKAEIAELLQKAEQADQAEDHRFGPHRRGDELPEELRFRERRLAKIQEAKAALEAEARLRAGADEHEPPEEPGPKGPKGGRRRKRPKAEPAPTAQRNFTDPDSRIMKGSDKQFIQGYNAQAVVDADTQIVVAAAVTNQAADAPHLIPMMEQVETNVDEMPEEVSADAGYFSEQNVQWLLQRRINPYIPPEKQRHSERRRAKSPRGPIPKGATLRDRMRRKLQLKRHAERYRKREQSIEPVFGQIKEARGFRQFHLRGLEAVNGEWLLVCLCHNLLKLYAFSQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J74</accession>
    <name>Q67J74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Formate dehydrogenase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH3295</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q67J74"/>
    <dbReference type="STRING" id="292459.STH3295"/>
    <dbReference type="EnsemblBacteria" id="BAD42276">
        <property type="protein sequence ID" value="BAD42276"/>
        <property type="gene ID" value="STH3295"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3295"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_9"/>
    <dbReference type="OMA" id="NVGTDIP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02790">
        <property type="entry name" value="MopB_CT_Formate-Dh_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041925">
        <property type="entry name" value="CT_Formate-Dh_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="3">
        <location>
            <begin position="6"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="4">
        <location>
            <begin position="417"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD42276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="531" mass="58709" checksum="4700FEBD6B49C9E9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J89</accession>
    <name>Q67J89_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphocarrier protein HPr</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197392.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3280"/>
    <dbReference type="EnsemblBacteria" id="BAD42261">
        <property type="protein sequence ID" value="BAD42261"/>
        <property type="gene ID" value="STH3280"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3280"/>
    <dbReference type="eggNOG" id="COG1925">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136230_2_2_9"/>
    <dbReference type="OMA" id="APHGIHT"/>
    <dbReference type="OrthoDB" id="404807at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001020">
        <property type="entry name" value="PTS_HPr_His_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002114">
        <property type="entry name" value="PTS_HPr_Ser_P_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00369">
        <property type="entry name" value="PTS_HPR_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00589">
        <property type="entry name" value="PTS_HPR_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPr" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9455" checksum="743A49EA9FF7CF9A" modified="2004-10-11" version="1">MVERTFTLSNPTGLHARPAALFVKEVQKFADTEIFVRKGEKEVSARSLLSLLSLGVAQGETIVIRCQGPREEEAMAALAALIEGGLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J93</accession>
    <name>Q67J93_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AcrB family membrane transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197388.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3276"/>
    <dbReference type="EnsemblBacteria" id="BAD42257">
        <property type="protein sequence ID" value="BAD42257"/>
        <property type="gene ID" value="STH3276"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3276"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_9"/>
    <dbReference type="OMA" id="FIIPCFY"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="849"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="879"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="905"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="951"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1028" mass="110106" checksum="EED63FA0BCBB1208" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC5</accession>
    <name>Q67JC5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonine ammonia-lyase</fullName>
            <ecNumber evidence="4">4.3.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197356.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3243"/>
    <dbReference type="EnsemblBacteria" id="BAD42225">
        <property type="protein sequence ID" value="BAD42225"/>
        <property type="gene ID" value="STH3243"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3243"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_1_9"/>
    <dbReference type="OMA" id="LIHPFDH"/>
    <dbReference type="OrthoDB" id="1876944at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005789">
        <property type="entry name" value="Thr_deHydtase_catblc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01127">
        <property type="entry name" value="ilvA_1Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="328"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="410" mass="43656" checksum="D8C73A6B2418E470" modified="2004-10-11" version="1">MTVTFADIEKARILLSGHIKRTPMLQSPGITEHLGLPVYLKAENLQRAGSFKVRGALNKIHSLTEEEKARGVIAASAGNHAQGVALAASSLGIRSVICMPEGAPIAKLEATQGYGAQVVLHGETYDDAYQKARALQAEHGYTFIHGFDDPYTIAGQGTVGLEIVEDLPDIATVVVPIGGGGLMAGVALAVKSLKPGVRVVGVQAAGAPAVFLSRQQGKLVATDSVHTIADGIAIKKPGALCWEIIREHVDDIVLVDEEEIAQSILFLLERAKVIVEGAGAVGMAALLGDKIPDLQGPVCVVLSGGNIDVNIISRIIERGLVKAGRYIRISTFVPDRPGGLQQLLATVAQAGANVIDVRHERWLNKVTIGEVEITLALETRNARHVDEILATLKAKGYHVWIIPPYRPNDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE6</accession>
    <name>Q67JE6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3222"/>
    <dbReference type="EnsemblBacteria" id="BAD42204">
        <property type="protein sequence ID" value="BAD42204"/>
        <property type="gene ID" value="STH3222"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3222"/>
    <dbReference type="eggNOG" id="COG3221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051472_4_0_9"/>
    <dbReference type="OMA" id="ANARMYS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="334" mass="36876" checksum="B4A5B113196A5423" modified="2004-10-11" version="1">MPHSRQVRKNPDEKYQPLARSRWRWMRVRRPLSVLLLAAVVSLWGCASAPQLPEVRLNQLDRTLAAGSVGISPRPVLKVGLATMMSPRESLLRYGPLLDYLNSKLERHVEVVLASSQTEMLELLRTGGVQLGLVGSLAYVQGADGFGLKALAVPEYQGEPTHRSLILVRRYSGLEEFAHLKGHTFAYTDPVSATGWLYPQTLVLELNEAVERFFDRALFTGTDDKAIQALDHGLVDGAAVNSVIYDMAVAEDPDLARRLRAIGSSEPLGSPPVVVSPTVGSELVAQLESVFLQMGEDEEGRAVLQSLRLDRFVAPDPAWYEPIRLMLEEVEASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH3</accession>
    <name>Q67JH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197308.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3195"/>
    <dbReference type="EnsemblBacteria" id="BAD42177">
        <property type="protein sequence ID" value="BAD42177"/>
        <property type="gene ID" value="STH3195"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3195"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052317_0_0_9"/>
    <dbReference type="OMA" id="FFEHLLF"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="1">
        <location>
            <begin position="63"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="2">
        <location>
            <begin position="182"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="428" mass="48272" checksum="074A87A3417CA816" modified="2004-10-11" version="1">MERHFDPILRDELYTERLENGLTVAVLVKPGFRQATGRVAVQYGSIDSCFVDPQSGDEVQVPDGIAHFLEHKLFEGPDGNVADRFAELGADVNAYTTHTHTVYYFTTTDHFAACLDLLLNFVQEPYFTPESVAREQGIIEQEIRMYLDDPGWRSSANLMEALFVRHPVRLDIAGTVESIRRIDQDLLYLCHRIFYHPSNMVLFVAGDLDPRAVVEQARAAFAGRRYPAQAPIQRRLPEEPQAIAQRRRVQELVVSQPIFRLGFKEKQVGLTGRPLLERDLLTAILLDVLVGKGSPLYTRLYESGLIDQRFGFGHAPEVTFGYTYVSGPTPDPEQLEAELLEGLARAREEGIQPEDFERARRKLVGRILNLMNDLEGLSYLFIDGFFKGIGLFDEIPALQSLTLDAANQRLREHFDAALAATSVISPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS0</accession>
    <name>Q67JS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate dehydrogenase associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3098"/>
    <dbReference type="DNASU" id="2982465"/>
    <dbReference type="EnsemblBacteria" id="BAD42080">
        <property type="protein sequence ID" value="BAD42080"/>
        <property type="gene ID" value="STH3098"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3098"/>
    <dbReference type="eggNOG" id="COG1526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_4_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="26743" checksum="71A737CDC9C049C9" modified="2004-10-11" version="1">MYSDQLVCPAHVPRGSPEGRVDAIAAMVTTRTAVQAPVERRTALFVNGQAWLTAQTTPHDLADWAVGRLCGEGLIAGPEEIAHLAVDEAGPCVHAVLPRVRTPAPPAPVAPGPRVAEAQLLRWMQEMLAQAPLYRATGGMHVAMAVRADSGEQIVREDIGRHQAVDKVLGAARLAGWPPAQVVVLTSGRISAEMCARVARFGAPVCASRTAATDEAWALATRLGMDLVGYVRTAASLIVYTTGNRVARRIEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU1</accession>
    <name>EFTU_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">STH3077</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197192.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU1"/>
    <dbReference type="SMR" id="Q67JU1"/>
    <dbReference type="STRING" id="292459.STH3077"/>
    <dbReference type="PRIDE" id="Q67JU1"/>
    <dbReference type="EnsemblBacteria" id="BAD42059">
        <property type="protein sequence ID" value="BAD42059"/>
        <property type="gene ID" value="STH3077"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3077"/>
    <dbReference type="eggNOG" id="COG0050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_9"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="621774at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
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        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
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    <dbReference type="Pfam" id="PF00009">
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    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000015765" description="Elongation factor Tu">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="10"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="60"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="395" mass="43579" checksum="2ED200F20DA767B8" modified="2004-10-11" version="1">MAKQRFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGKAQFMAYDAIDKAPEERERGITINTSHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDEELLELVELEVRELLNQYEFPGDEIPFIRGSALKALEGDPKYVKAIEELMDAVDSYIPTPQRDADKPFLMPIEDVFTITGRGTVVTGRVERGKCKVGDQVEIVGLREESKTTVVTGLEMFRKILDEVQAGDNVGALLRGIEKKEVERGQVLAKPGSIKPHTKFSGAIYVLTKEEGGRHSPFFNGYRPQFYFRTTDVTGTIKLPEGVEMVMPGDNVEIAVELIHPIAIEEGLRFAVREGGRTVASGRVTKVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K14</accession>
    <name>Q67K14_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Flagellar M-ring protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">fliF</name>
        <name evidence="14" type="ordered locus">STH3003</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The M ring may be actively involved in energy transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FliF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3003"/>
    <dbReference type="EnsemblBacteria" id="BAD41986">
        <property type="protein sequence ID" value="BAD41986"/>
        <property type="gene ID" value="STH3003"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3003"/>
    <dbReference type="eggNOG" id="COG1766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028108_2_0_9"/>
    <dbReference type="OMA" id="NYEVNRI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009431">
        <property type="term" value="C:bacterial-type flagellum basal body, MS ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013556">
        <property type="entry name" value="Flag_M-ring_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000067">
        <property type="entry name" value="FlgMring_FliF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006182">
        <property type="entry name" value="FliF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043427">
        <property type="entry name" value="YscJ/FliF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30046">
        <property type="entry name" value="PTHR30046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30046:SF0">
        <property type="entry name" value="PTHR30046:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01514">
        <property type="entry name" value="YscJ_FliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08345">
        <property type="entry name" value="YscJ_FliF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004862">
        <property type="entry name" value="FliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01009">
        <property type="entry name" value="FLGMRINGFLIF"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00206">
        <property type="entry name" value="fliF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="14" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="YscJ_FliF" evidence="12">
        <location>
            <begin position="45"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="YscJ_FliF_C" evidence="13">
        <location>
            <begin position="251"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="287"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD41986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="576" mass="61564" checksum="F78D15B178BB9E58" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K38</accession>
    <name>Q67K38_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar hook-associated protein 2</fullName>
            <shortName evidence="5">HAP2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Flagellar cap protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliD</name>
        <name evidence="8" type="ordered locus">STH2977</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FliD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197092.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2977"/>
    <dbReference type="EnsemblBacteria" id="BAD41960">
        <property type="protein sequence ID" value="BAD41960"/>
        <property type="gene ID" value="STH2977"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2977"/>
    <dbReference type="eggNOG" id="COG1345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015182_8_1_9"/>
    <dbReference type="OMA" id="TLEWQRD"/>
    <dbReference type="OrthoDB" id="883503at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009421">
        <property type="term" value="C:bacterial-type flagellum filament cap"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010810">
        <property type="entry name" value="Flagellin_hook_IN_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040026">
        <property type="entry name" value="FliD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010809">
        <property type="entry name" value="FliD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003481">
        <property type="entry name" value="FliD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30288">
        <property type="entry name" value="PTHR30288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07196">
        <property type="entry name" value="Flagellin_IN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07195">
        <property type="entry name" value="FliD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02465">
        <property type="entry name" value="FliD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="FliD_N" evidence="6">
        <location>
            <begin position="10"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="FliD_C" evidence="7">
        <location>
            <begin position="227"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="476" mass="52044" checksum="7FEC495F2DC12063" modified="2004-10-11" version="1">MSMRLGGLISGADTETLISALLELERVRIYRQEEKQEQLEAKQKAWRDVRSALTNIQSKLDQLRFPTLFRSRKVELSDNSVATVTADAGAAQTSYTLQVVKLAQSHVITTQSEAAYKAADEQLGWAGTFDIGTDPNALKTITVEETDTLSSLAAKINAADSGVLAHVVMVSEGKFRLVLTAAKSGSANVIRLENEVPGQPPQGQTYGNLLEDILGLTGANQLVLSEAQDAEIILNGEPYKSSTNTFDNILPGIKITVKKPSGDDVVSMTVSSDIDKIVQVVKDWVNAVNSLQDLLKKLSAYDVEKKTGAALTGESLVRSIQSRMRQFFSTEVAGMPASMKMLSQIGVSTGAYGTADFGKIVVDEEKLREVLQRDPEGVARLFGLNEDGQKGIAVQMNDYIKSLLDSQSGALEVRDKSLSQQIDRIRDTIERIEVQLEQREKVLRLQFARMEEAMARLQGQGNYLALMLLGRSQSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K75</accession>
    <name>Q67K75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2940"/>
    <dbReference type="EnsemblBacteria" id="BAD41923">
        <property type="protein sequence ID" value="BAD41923"/>
        <property type="gene ID" value="STH2940"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2940"/>
    <dbReference type="HOGENOM" id="CLU_1546799_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="18703" checksum="D123533956E2831B" modified="2004-10-11" version="1">MGDYRRCLAHGRRLLKAGVLTPGERLRVLVLVERCRRATGSVRQICSARLRLVSAGMLRAAGRPQQAVDEAFRALQEAEPLSPLQVRAQLFLSRVAGATGRLVEALAFALAARVTAATCGMGWLERRASDLLALQLRAGGWTAVSELASALAAQGVDVYEYLDLADLISFARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K89</accession>
    <name>Q67K89_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2926</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197042.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2926"/>
    <dbReference type="EnsemblBacteria" id="BAD41909">
        <property type="protein sequence ID" value="BAD41909"/>
        <property type="gene ID" value="STH2926"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2926"/>
    <dbReference type="eggNOG" id="COG0802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087829_3_0_9"/>
    <dbReference type="OMA" id="FTDDAIC"/>
    <dbReference type="OrthoDB" id="1827295at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17424" checksum="1E37C6F72C6A0DD4" modified="2004-10-11" version="1">MVRVISRSPAQTQALGRWLGERLQPGDFVALVGDLGTGKTALSTGILAGLGVSRSGGSPTFTLLWEYEGRIPVFHWDVYRLEDAAELEDLGFEEYFFSDHGVNLVEWADRVRPLWPDEVLEISLSYGSGEQERVLELTGTARYRPLLEELAHAGFGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC3</accession>
    <name>ARGC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="1">AGPR</shortName>
            <ecNumber evidence="1">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argC</name>
        <name type="ordered locus">STH2892</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197009.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KC3"/>
    <dbReference type="SMR" id="Q67KC3"/>
    <dbReference type="STRING" id="292459.STH2892"/>
    <dbReference type="EnsemblBacteria" id="BAD41875">
        <property type="protein sequence ID" value="BAD41875"/>
        <property type="gene ID" value="STH2892"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2892"/>
    <dbReference type="eggNOG" id="COG0002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_9"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="OrthoDB" id="951261at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000011071" description="N-acetyl-gamma-glutamyl-phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <sequence length="348" mass="37486" checksum="48139F0629261DE7" modified="2004-10-11" version="1">MTIRVGIAGATGYTGVELVRLLAQHPEAEVVVAGTESYQGQHLAAVYPHLRERVDLVGREASPEALAGCDVVFTSLPHGLTMALAPAVLAAGGRLIDLGADFRLRDVTAYEQWYRKTHTAPDLMEEAVYGLPELYRERIRGARLVGNPGCYPTACALAAAPLLQAGVVETEGIIFDAKSGVSGAGRGVNLGVHFSEVNENFKAYNIAGTHRHTPEIEQTLSDLAGRPVVVSFTPHLVPMTRGILATGYFTLKAERTTEQLVDLFREFYAGEPFVRVRPAGELPTTKQVWGSNYCDIGLQVDPRTRRVLVISVIDNLVKGAAGQAIQNMNLLFGLPETTGLLNAGPVYP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC5</accession>
    <name>ARGJ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">Ornithine acetyltransferase</fullName>
                <shortName evidence="1">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglutamate synthase</fullName>
                <shortName evidence="1">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">argJ</name>
        <name type="ordered locus">STH2890</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="1"/>
    <dbReference type="EC" id="2.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197007.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KC5"/>
    <dbReference type="SMR" id="Q67KC5"/>
    <dbReference type="STRING" id="292459.STH2890"/>
    <dbReference type="MEROPS" id="T05.002"/>
    <dbReference type="EnsemblBacteria" id="BAD41873">
        <property type="protein sequence ID" value="BAD41873"/>
        <property type="gene ID" value="STH2890"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2890"/>
    <dbReference type="eggNOG" id="COG1364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_9"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="OrthoDB" id="1083409at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000227264" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000227265" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="1">
        <location>
            <begin position="189"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <sequence length="402" mass="41138" checksum="5259A6FBDF381D70" modified="2004-10-11" version="1">MEWLAGGVTAPAGFVAAGACADIKGNGAGKKDVALLASRVPCAAAGVYTTNLVKAAPVVLTRGRTETGELQAVVANSGNANACTGEQGMRDAAEMARLAAEALGIRPELMGVASTGVIGVPLPMDRVSAGIRAAAAALSPEGGADAAEAIMTTDTFPKQAAARLEIGGATVTIGAMAKGSGMIHPNMATMLGFVTTDAAVDAAALREALREATDRSFNMITVDGDTSTNDMVLVLANGLAGNPRIMPGSAHYRAFADALAAVLIHLAKEIARDGEGATKLIEVRVKGAATLSDARKAARAVCGSNLVKAAVFGEDANWGRVLAALGYSGAQFDPGRVDLWLGDLQMMRAGEPVAFDEAAAARVLREKEVVFTADLHAGECEATAWGCDLTYDYVKINGSYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ3</accession>
    <name>Q67KQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2759"/>
    <dbReference type="EnsemblBacteria" id="BAD41744">
        <property type="protein sequence ID" value="BAD41744"/>
        <property type="gene ID" value="STH2759"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2759"/>
    <dbReference type="HOGENOM" id="CLU_3030778_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="55" mass="5608" checksum="F0E86B6622161DEB" modified="2004-10-11" version="1">MAMTLLDSGNLFLTWLFVARRSLPGGVLVLSGDVLLGSLLLGRGGLPAIAGSRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT6</accession>
    <name>Q67KT6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2725</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2725"/>
    <dbReference type="EnsemblBacteria" id="BAD41710">
        <property type="protein sequence ID" value="BAD41710"/>
        <property type="gene ID" value="STH2725"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2725"/>
    <dbReference type="eggNOG" id="COG3601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086673_2_2_9"/>
    <dbReference type="OMA" id="MFLEFPI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025720">
        <property type="entry name" value="RibU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38438">
        <property type="entry name" value="PTHR38438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037778">
        <property type="entry name" value="UCP037778_transp_RibU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="17997" checksum="95496BC5E78A7A2A" modified="2004-10-11" version="1">MFFGEIYVPPFAEFLRYDPGDIPAIVATYTLGPAAGVAIQGIKVGLFILSGKGSTGWIGGLANFTAGSALVIAAGVSHRLFDRAGLKHWIWTFLSAVIGTVIMAAILIPANALFIYPLWGMQGTAAWVGALTLSTPFNLFKGMLSTSISLAFYRRLEPFLLGRAADRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU4</accession>
    <name>Q67KU4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2717</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41702">
        <property type="protein sequence ID" value="BAD41702"/>
        <property type="gene ID" value="STH2717"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2717"/>
    <dbReference type="HOGENOM" id="CLU_2439682_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="90" mass="9820" checksum="C16AF07E34BA2D82" modified="2004-10-11" version="1">MVVATILLTTVTISHAMLSFGGARHSRPHRSRRTCHSYVASMEVDVSFGLLCAVHRSQVETLNHEPYLVVGMPHRSPEPGLGVDLEGCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW7</accession>
    <name>Q67KW7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stage V sporulation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoVB</name>
        <name evidence="8" type="ordered locus">STH2694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2694"/>
    <dbReference type="EnsemblBacteria" id="BAD41679">
        <property type="protein sequence ID" value="BAD41679"/>
        <property type="gene ID" value="STH2694"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2694"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_2_1_9"/>
    <dbReference type="OMA" id="AYPIYTT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13124">
        <property type="entry name" value="MATE_SpoVB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024923">
        <property type="entry name" value="PG_synth_SpoVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038958">
        <property type="entry name" value="PG_synth_SpoVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="353"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="523" mass="54996" checksum="5F464792D6F59209" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY9</accession>
    <name>Q67KY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196794.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2672"/>
    <dbReference type="EnsemblBacteria" id="BAD41657">
        <property type="protein sequence ID" value="BAD41657"/>
        <property type="gene ID" value="STH2672"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2672"/>
    <dbReference type="eggNOG" id="COG1764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1721435_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="16401" checksum="0A9B0AC77DE1C591" modified="2004-10-11" version="1">MHDGSLTLRAYASWKGVTATSGEVSSSFSNLRLAFTAPVADGGEPGLTSPEEMLLAAAATGWITAFLDVARQMVLDVADVAVEGELVRTYDELEGTRIERIALKPEVKLRSGLGFEKVHDMFRRATALARQRSPVLRALAAGVQLEVDPSYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L18</accession>
    <name>Q67L18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative (2R)-phospho-3-sulfolactate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2643"/>
    <dbReference type="EnsemblBacteria" id="BAD41628">
        <property type="protein sequence ID" value="BAD41628"/>
        <property type="gene ID" value="STH2643"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2643"/>
    <dbReference type="eggNOG" id="COG1809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062679_2_0_9"/>
    <dbReference type="OMA" id="IPPYKWI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="SSF102110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="262" mass="28135" checksum="140DC2478E880B94" modified="2004-10-11" version="1">MIRDPLPGRARKPRSVGLTMVIDKGLGLGPTRALLELAGEYIDFIKLGFGTAALYPERILREKVALIRGCGVEPYPGGTFLEAALLQGRLEAFLAATWDLGFRAVEVSEGTIDLDHRDRERAIRAARNMGFTVITEVGKKDGSQELDPAAALSRIEADLAAGAAKVIVEGRESGKGVGLYDREGRLKLGLLERLVAGLPDPGAIIWEAPLKAQQEELIARFGVNVNLGNVPPEEVIALEALRCGLRGDTLRLAIEGARPAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L29</accession>
    <name>Q67L29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2632</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2632"/>
    <dbReference type="EnsemblBacteria" id="BAD41617">
        <property type="protein sequence ID" value="BAD41617"/>
        <property type="gene ID" value="STH2632"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2632"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022800_1_1_9"/>
    <dbReference type="OMA" id="IGANSKM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027022">
        <property type="entry name" value="ABC_permease_BceB-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018968">
        <property type="entry name" value="ABC_permease_BceB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="548"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="89"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="681" mass="73251" checksum="02EE4E4152AF07EC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L34</accession>
    <name>Q67L34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine betaine/carnitine/choline ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196749.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2627"/>
    <dbReference type="EnsemblBacteria" id="BAD41612">
        <property type="protein sequence ID" value="BAD41612"/>
        <property type="gene ID" value="STH2627"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2627"/>
    <dbReference type="eggNOG" id="COG1732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038355_1_0_9"/>
    <dbReference type="OMA" id="KDPAWKN"/>
    <dbReference type="OrthoDB" id="770506at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269522" evidence="2">
        <location>
            <begin position="27"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="OpuAC" evidence="3">
        <location>
            <begin position="33"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="304" mass="33477" checksum="844CFD610D1566D0" modified="2004-10-11" version="1">MFGHRRFSAVAAAVLCVLLAGCGGGAGSGAGDAIVVSSKDFTESILLGEIAAQLIESRTDLTVVRKHNLGGTTVNFNGLKNGDITLYAEYDGTAYGPILGHTEPIADPDGLHDLVKREFEEKFGITWTAPLGMNNTYTLAVPREIAEQHNLKTFSDLLAVDDQLVFGTTNEFMGRQVDGYYPLVEAYGFAFKDVKTMQTGLRWKAIETGEIQVIDAYATDGKLVEFDMVILEDDRNFFPPYNGAYVVRMDAAERHPEIIELLNQLGGLLPDEKMQELNYRVESLEEPVEEVARDFLVEAGLVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L62</accession>
    <name>Q67L62_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative cytochrome Ni/Fe component of hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2599"/>
    <dbReference type="EnsemblBacteria" id="BAD41584">
        <property type="protein sequence ID" value="BAD41584"/>
        <property type="gene ID" value="STH2599"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2599"/>
    <dbReference type="eggNOG" id="COG5557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049007_0_0_9"/>
    <dbReference type="OMA" id="AIEVCAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005614">
        <property type="entry name" value="NrfD_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03916">
        <property type="entry name" value="NrfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="39831" checksum="1A38FDBE946B7244" modified="2004-10-11" version="1">MGLGAATGLNDSMPWGLWKIYDVVVVVPLGATGFVMAFIRYFTPGGERYEYLNRRAVIWAAIMYLSMGVRLAFDIGIPWRIIYPIVRGGNLHSPLFEIAWCVFLYLIVLFFENISRVAEEQKRPWVKKADHLLHKILPGFVLVGVMLSTMHHSSLGTLFMITGKRMDPLWYHPWLNYVFLLTSIATGMGMMMVIEHLTSRYYKIKFETDLLARLAPWVAGILSFVLAWRLGAMAAEGQIGQIFVPRVETALWWAEILLGYVAPIAILFSRARFTQKGLFAGGLLTVLGMIALRLNVGFTALMDALGAQYTPSVPEILFSVGATAGTVVIYTWFVETLPSILGPRERETEGLEAVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L96</accession>
    <name>Q67L96_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DSBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2565"/>
    <dbReference type="EnsemblBacteria" id="BAD41550">
        <property type="protein sequence ID" value="BAD41550"/>
        <property type="gene ID" value="STH2565"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2565"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1844092_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="1">
        <location>
            <begin position="58"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15578" checksum="1F6AE1EB2797AF22" modified="2004-10-11" version="1">MARAGEIRYEVHYLPIFPATGPAIFAVQCAGEQGYWWAMHGRILEDQTRGIRAVQTLDDLDALLARYAEELGLDMAAYRQCLASEEKVQGYIDRVNDQIAAAQDLGIRGTPTFVINGEPVEMDSFDDILSAVREELKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB0</accession>
    <name>Q67LB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196674.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2551"/>
    <dbReference type="EnsemblBacteria" id="BAD41536">
        <property type="protein sequence ID" value="BAD41536"/>
        <property type="gene ID" value="STH2551"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2551"/>
    <dbReference type="eggNOG" id="COG4347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103291_1_0_9"/>
    <dbReference type="OMA" id="TVYGYYW"/>
    <dbReference type="OrthoDB" id="1223056at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009845">
        <property type="entry name" value="DUF1405"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40042">
        <property type="entry name" value="PTHR40042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07187">
        <property type="entry name" value="DUF1405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="23165" checksum="D4844A0070AEF7C7" modified="2004-10-11" version="1">MTWLQGFLRSVAHDRRWWWALLVINFLGSLYGFYWYWPQLSQTPPARWFIVPDSPGATFLFAIWLGLLLAGVDWRSPGMQLLGAVAFVSNMKYGLWTATVLPQAGMKYGWEFDFVHLSLSHLGMWVQGFLFARHYRPGPAAAAVALAWMVVQDTVDYRLWMTHPTLPYQAEFAFARGAAVALSLIWGLFLLGQSLSSRRHVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB9</accession>
    <name>Q67LB9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="3">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2542</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the citrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196665.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2542"/>
    <dbReference type="EnsemblBacteria" id="BAD41527">
        <property type="protein sequence ID" value="BAD41527"/>
        <property type="gene ID" value="STH2542"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2542"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022049_2_1_9"/>
    <dbReference type="OMA" id="NEAVMHM"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019810">
        <property type="entry name" value="Citrate_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11739">
        <property type="entry name" value="PTHR11739"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00143">
        <property type="entry name" value="CITRTSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00480">
        <property type="entry name" value="CITRATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="439" mass="49810" checksum="23DCF3C2DB322809" modified="2004-10-11" version="1">MSQLKATLVRKIEEHRPRTQRLLKEFGHVVIDEVTIEKAIGGARDVRCLVTDISYLDPMEGIRFRGKTIEETFAALPKAPGGEYPTVEAFWFFLLTGEVPTQEQVDEVIAEWKERRNVPQYVYDVIRALPKDSHPMTMLSAGILAMQRESKFARYYHSGEYNKQRAWEYMYEDASDLVARIPMIAAYIYRYKYKDDVHIPPDPNLDMGANFAHMMGVPKPYDDVSRMYFILHSDHESGNVSAHTGHLVASALSDAYLAYSAALNGLAGPLHGLANQEVLRWIMDFQAKLGEGVEPTRENVTQALWDTLNAGQVIPGYGHAVLRKTDPRYTAQREFCLKHMPDDPLFKLVSMIYEVAPGVLMQHGKAKNPWPNVDAQSGVIQWHYGVREWDFYTVLFGVGRALGVMANIVWDRALGYPIERPKSVTTEMLEQWAAAGGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF1</accession>
    <name>Q67LF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2510</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2510"/>
    <dbReference type="EnsemblBacteria" id="BAD41495">
        <property type="protein sequence ID" value="BAD41495"/>
        <property type="gene ID" value="STH2510"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2510"/>
    <dbReference type="eggNOG" id="COG3307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429550_0_0_9"/>
    <dbReference type="OMA" id="WETEAYA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="637" mass="66167" checksum="C93ED96EBA2BBC1B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG2</accession>
    <name>Q67LG2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2499"/>
    <dbReference type="EnsemblBacteria" id="BAD41484">
        <property type="protein sequence ID" value="BAD41484"/>
        <property type="gene ID" value="STH2499"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2499"/>
    <dbReference type="eggNOG" id="COG2871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019091_0_0_9"/>
    <dbReference type="OMA" id="GNVMDHL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027980">
        <property type="entry name" value="RACo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041414">
        <property type="entry name" value="Raco_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042259">
        <property type="entry name" value="Raco_middle_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14574">
        <property type="entry name" value="RACo_C_ter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17651">
        <property type="entry name" value="Raco_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="508" mass="52126" checksum="7D3F82DB5FCDF85C" modified="2004-10-11" version="1">METPCGARGQCRKCLVRVLGEAPEPTPADLEQLTPAELAQGFRLSCQLRVDRDLEVAVVPAVTLDPRKARLGALAGPAEVDPWAPLNPTGRSLGFALDVGTTGIAGALLDLRTGEELAVHASLNPQAVYGADLMTRLSHAIQGEEQKGELTRLVRDAAQALLGRLLGKAGARPEEVTAASLVGNTAMHHLFLGLPVEQLAVAPYTPAVTEGRVFEVPGFPALYALPVIAGFVGADTVAAALAAGLDAGEETVLLVDVGTNGEVLLRHAGRLYACSAPAGPAFEGGEISQGMRAGPGAIEAVRFDGRDLVVTVIPGGPRSTAGGGGGSGTEAARGICGSGLLDAAAALLEAGLMDETGRLTHAGPAAHRLAEGGRAVALAPGVQLTQKDLRSLQLAKGAIRSGIEVLLRVAGIGPARLDKVLLAGAFGNYLRRESAVATGLLPPVDPGRIQPIGNAAIAGAKLALLSRSARDRAEALARGARFVELATHPDFEEVFMEALNFPRVRPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG4</accession>
    <name>Q67LG4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RraB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196620.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2497"/>
    <dbReference type="EnsemblBacteria" id="BAD41482">
        <property type="protein sequence ID" value="BAD41482"/>
        <property type="gene ID" value="STH2497"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2497"/>
    <dbReference type="HOGENOM" id="CLU_2071966_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036701">
        <property type="entry name" value="RraB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009671">
        <property type="entry name" value="RraB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06877">
        <property type="entry name" value="RraB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89946">
        <property type="entry name" value="SSF89946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RraB" evidence="1">
        <location>
            <begin position="14"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="118" mass="13354" checksum="27741F3BCF1A0F83" modified="2004-10-11" version="1">MRPETEAERAARHAATIQAFRALVDPAHPPREPFVLEQHFYAAEGGDVDGLAAALEERGFKVESLVYDPDNADRAWRLVAVRLELLEERRLLAVSDELDALARRFDAMYDGWLTHVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL9</accession>
    <name>Q67LL9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="10" type="ordered locus">STH2442</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196565.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2442"/>
    <dbReference type="EnsemblBacteria" id="BAD41427">
        <property type="protein sequence ID" value="BAD41427"/>
        <property type="gene ID" value="STH2442"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2442"/>
    <dbReference type="eggNOG" id="COG0341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050012_0_0_9"/>
    <dbReference type="OMA" id="AFEWRMA"/>
    <dbReference type="OrthoDB" id="410730at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00966">
        <property type="entry name" value="3a0501s07"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="33303" checksum="32083DB4D6ED2B59" modified="2004-10-11" version="1">MGLTRIRYMRYARHYLIFTLVTVLLAAGALLFRGLNLGIDFTGGLLLDLQFERTVTIEQVRTVIDSALGIDAQIQQVEPKGAASPEATEFLVRTPELSAEDKDRLYGSLLHLGAYTVIGEDAVSGTVSSELTNKALLAVGIAAVLQVIYISIRFQFRMGVAAVVALLHDVLLTMGVLALFQVEISSNFVAAILTVLGYSMNDTVVVLDRLRENLHSRQKGESLEEMATRSIQEVITRSIYTGVSVQLMLLAMIFLGGSTVLDLASTLFVGILAGTYSSITVAAALWFVWEQSREGGHPGPAGARPARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN3</accession>
    <name>Q67LN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Zn-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196551.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2428"/>
    <dbReference type="EnsemblBacteria" id="BAD41413">
        <property type="protein sequence ID" value="BAD41413"/>
        <property type="gene ID" value="STH2428"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2428"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_4_9"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="OrthoDB" id="1581636at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="12"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="22003" checksum="CF57333AA7CE0B47" modified="2004-10-11" version="1">MQLWHRALGAFMANCYIVACGETRQALVIDPGVPDPWIQSCVMENDLTVVGIVLTHGHLDHIGGVDWVKGWSGAPVAIHEADADCLTSPARNGSLYFGMTVQASPADRLLRDGDEVEAGGLRFRVIHTPGHSPGGICLYRPGHLLAGDTLFAGSIGRTDLPGGDYHTLIQSIKERLLTLPPDTAVYPGHGPSTTIGDEKAYNPFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV4</accession>
    <name>Q67LV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA dehydrogenase, short-chain specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196480.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2357"/>
    <dbReference type="EnsemblBacteria" id="BAD41342">
        <property type="protein sequence ID" value="BAD41342"/>
        <property type="gene ID" value="STH2357"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2357"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_2_9"/>
    <dbReference type="OMA" id="NPPMDDM"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013107">
        <property type="entry name" value="Acyl-CoA_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08028">
        <property type="entry name" value="Acyl-CoA_dh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="5">
        <location>
            <begin position="12"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="4">
        <location>
            <begin position="130"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_2" evidence="6">
        <location>
            <begin position="253"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="401" mass="42747" checksum="EEE6E288C7BFEE31" modified="2004-10-11" version="1">MAVPEPDLFVRTERQRRFVALAGELADRFAGRAALHDRENSFPFENFAELRASGYVRLTVPVEWGGEGANLTETLLAQERLAQGDGATALGIGWHLAVVGKLAETRAWPPAATARIFREVAGRGALINSAASEVETGSPSRGGRPTTTARRAPGGWLLTGRKSFTSLAPVLDYAVVSASFEGSDGGGWFLVQTGSPGVTVVETWDALGMRATGSHDLVLEDVFVEDGDLLETFGRGNTCQVGTGDGAGWALHIPAVYLGIARAAHGFAVEYARTRRPNSLPGPIADQPHIQALLGQNEIDLLAARSALYTVADRWDAEPERRGELVPLLGAAKVLAVNLALQIVDRAMRVAGIAGLFRQLPLERLYRDVRAGLHNPPMEDAVLRNLARAALARQASAAAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M17</accession>
    <name>Q67M17_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2293</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2293"/>
    <dbReference type="EnsemblBacteria" id="BAD41278">
        <property type="protein sequence ID" value="BAD41278"/>
        <property type="gene ID" value="STH2293"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2293"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_3_3_9"/>
    <dbReference type="OMA" id="GAFRIVC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="60"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="273"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="898" mass="99966" checksum="82039D7B6876BCA3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M23</accession>
    <name>Q67M23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS21 family transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2287"/>
    <dbReference type="EnsemblBacteria" id="BAD41272">
        <property type="protein sequence ID" value="BAD41272"/>
        <property type="gene ID" value="STH2287"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2287"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_1_2_9"/>
    <dbReference type="OMA" id="ACQRMIP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="51"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="209" mass="24119" checksum="41CA9AE14AB06C21" modified="2004-10-11" version="1">MIHREDNQLQRRLKAAGFPVPKTLESFDFTVMPSVNKAKILALSKSEYIRDRENILLVGNSGTGKTHLATALGIAACRQGYRVRFWRVGQFVAELQEAQNEHRLSRLEKQWLRFDLVVLDELGYVSLSRPASELLFHFVAARYERGSLIITTNLEFSEWPQVFGDQKMTAALADRVTHKAHIIEMNGESYRFRETLRRQEALKHHPETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M99</accession>
    <name>Q67M99_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2209"/>
    <dbReference type="EnsemblBacteria" id="BAD41194">
        <property type="protein sequence ID" value="BAD41194"/>
        <property type="gene ID" value="STH2209"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2209"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068384_0_0_9"/>
    <dbReference type="OMA" id="FMRREIR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="31525" checksum="0C668B2F8B0D25F7" modified="2004-10-11" version="1">MRKEFADHVSGWRMVILVLLLMVTGIGSLYTAAQTIRSVVGATAATPSFVFLRLFTGGSTQLPPLTWFMAFLAPLLGIALGFDAICGEQNRRTLSRVLAQPIHRDAVINGKFLAGLLSVTITFVALLLGVGGLGLIMLGVPPTGDELLRLVAYGVLTIIYVAFWLALAILFSVVFQNAATSALASIAVWLFFNVFWDLIVNVAAGDDPVGLGLWLMRLSPGYLYSEATVTLLTPKVRTLGAVTLEQVLGALEGVLSVDQSIMLIWPHAVGLVALTAVCFAVSYILFMRREIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA5</accession>
    <name>Q67MA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196328.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2203"/>
    <dbReference type="EnsemblBacteria" id="BAD41188">
        <property type="protein sequence ID" value="BAD41188"/>
        <property type="gene ID" value="STH2203"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2203"/>
    <dbReference type="HOGENOM" id="CLU_688725_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="41217" checksum="3405E9FBB2AC719D" modified="2004-10-11" version="1">MTQRERLQALCQAALAAAAGLQAMLGGAEIVLEDGPREVGRCGVGSRGVAPRGDSLRGASLAEAVLHLARSAAAHLPGRHAASPAEEAGHPPAQSAHRLVETVHLPPKAARQPVEAAPPPSEAAHRPGEAAHLPSEAAHRPGEAAHLPSEAAHRPVVAAHPPAKAAHPPAEGAHPPAEAAPAGQKGPALTLAIYPAPELAPLAAALGLDGEPLAPDGDHAADATTRELAWLPLLPGWSTYRTFPGSRLLAVAGYRRAALVAFEAAGRPAPMGRPPESHWQAQMWAPLGCDALAAALAPTERGPLFMEAPARLRDAIRTGRVRRALLGGADLAWALEQPGARAVRPLTGPGLDWMPVWGLFSRGVRPNLPDPPPELDAHLVLVPAEAVPGLTGWLEGAVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC5</accession>
    <name>Q67MC5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41168">
        <property type="protein sequence ID" value="BAD41168"/>
        <property type="gene ID" value="STH2183"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2183"/>
    <dbReference type="HOGENOM" id="CLU_1199305_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="25229" checksum="35AD0BFE8D855428" modified="2004-10-11" version="1">MPLQVLRASGLRSLVHAQNLEFGSGYHYRFCAGEAGGEAGCAQNLRFVRECHFWFCAQEGSGTSSTRKICGSARAITSGSARVRLLKRPAARKTCGSFPSTTSGSARKKAPEPRPRAESAVRFGLSLQVLRARGLRSLVHAQNLEFGSGYHYRFCAGEAGGGVVHANNLRFDPESHFRFCAHGASGTWLTRRICSWAWTTGSRFARKRDAVRCPRVKSAVRLGPSLQVLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME4</accession>
    <name>Q67ME4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2164</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196289.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2164"/>
    <dbReference type="EnsemblBacteria" id="BAD41149">
        <property type="protein sequence ID" value="BAD41149"/>
        <property type="gene ID" value="STH2164"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2164"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_2_9"/>
    <dbReference type="OMA" id="NAKVLAW"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="210"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="37249" checksum="81B4464D3BEF1FA2" modified="2004-10-11" version="1">MKANVAINGFGRIGRMVCRRLLRHPDLNLVAVNASYPAETLAHLLKYDTVHGRLDAQVVAGDGEIVVDGHVVRLVSERDASRLPWRELGIDIVIDATGKHKERERAALHLHSGARKVIITTATKDPDVTVVMGVNEGAYRPAEHHIIAAASCTTNCLAPVAKVLHETFGIRTGLMTTVHAYTNDQNMLDNPHRDLRRARAATASIIPTTTGAAKAVAQVLPELKGRLNGFALRVPTPDVSVVDLVVQLERPATREEINDALRSAAQGPLRGILAYSDEPLVSADFIGDPHSAIVDGTLTMVGPDNLAKVIAWYDNEWGYSCRVVDLAALVARQLALEPPLEGVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF4</accession>
    <name>Q67MF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2154"/>
    <dbReference type="EnsemblBacteria" id="BAD41139">
        <property type="protein sequence ID" value="BAD41139"/>
        <property type="gene ID" value="STH2154"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2154"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127498_0_0_9"/>
    <dbReference type="OMA" id="GRWLTHE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="21535" checksum="6A7DD937953FD75D" modified="2004-10-11" version="1">MAPGFPTRATRPSSGTRGGRRGNMHALVVGGTGMLREATLALTRRFATVSVVARRADAMPGRPAGINPVPVDYHDTGALAQALRRAIGQHGPVRLAVCWIHSTAPEALGVVAAEIGRSGEPCRLFHVRGSAAADPTRLPRPALEALPPNVAYRQVILGFQIEGERSRWLTHGEISQGVLQAIADDAEYHVVGVVRPWERRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML1</accession>
    <name>Q67ML1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="8">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196222.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2097"/>
    <dbReference type="EnsemblBacteria" id="BAD41082">
        <property type="protein sequence ID" value="BAD41082"/>
        <property type="gene ID" value="STH2097"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2097"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_3_9"/>
    <dbReference type="OMA" id="PVDFQYH"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014241">
        <property type="entry name" value="Cyt_c_oxidase_su1_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02891">
        <property type="entry name" value="CtaD_CoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="9">
        <location>
            <begin position="15"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="628" mass="69499" checksum="5B4B2016E1B8E763" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP5</accession>
    <name>Q67MP5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ethanolamine ammonia-lyase large subunit</fullName>
            <shortName evidence="1">EAL large subunit</shortName>
            <ecNumber evidence="1">4.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eutB</name>
        <name evidence="2" type="ordered locus">STH2063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds. Allows this organism to utilize ethanolamine as the sole source of nitrogen and carbon in the presence of vitamin B12.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ethanolamine = acetaldehyde + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15313"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="EC" id="4.3.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
        <text evidence="1">Binds between the large and small subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine degradation; ethanolamine degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The basic unit is a heterodimer which dimerizes to form tetramers. The heterotetramers trimerize; 6 large subunits form a core ring with 6 small subunits projecting outwards.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial microcompartment</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EutB family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196188.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2063"/>
    <dbReference type="EnsemblBacteria" id="BAD41048">
        <property type="protein sequence ID" value="BAD41048"/>
        <property type="gene ID" value="STH2063"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2063"/>
    <dbReference type="eggNOG" id="COG4303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048555_0_0_9"/>
    <dbReference type="OMA" id="EADQNDM"/>
    <dbReference type="OrthoDB" id="230269at2"/>
    <dbReference type="UniPathway" id="UPA00560"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008851">
        <property type="term" value="F:ethanolamine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00861">
        <property type="entry name" value="EutB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010628">
        <property type="entry name" value="EutB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044939">
        <property type="entry name" value="EutB_dom_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044941">
        <property type="entry name" value="EutB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39329">
        <property type="entry name" value="PTHR39329"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06751">
        <property type="entry name" value="EutB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018788">
        <property type="entry name" value="EutB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="1" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="1" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="423" mass="46530" checksum="9EF55BC693A4AB33" modified="2004-10-11" version="1">MILKTTLRGQVYQFRSVKEVLAKANELRSGDVLAGIAAETEAERVAAKHVLANLTLEDLRNNPVIPYEEDEVTRVIQDAVNEHVYRQIKGWTVAELREYILSDKADAQEIQRISRGLTSEMVAAVAKLCSNLDLIYGASKMHNVVQCNTTIGLPGTLAMRLQPNDPVDSIDGIMPQIMEGLSYGIGDAVIGINPVNDDPESVSRLLWAVYEFMEKWRIPTQQCVLAHVTTQMRCIRDGAPVGLVFQSIAGSEKAMEAFGVSVEMLDEALELVRKYGSAKGPNLMYFETGQGSELSADAHHGADQVTLEARCYGLAKRYQPLLVNTVVGFIGPEYLYDAKQVIRAGLEDVFMGKLTGISMGCDVCYTNHMKADQHDIENLAVLLTAAGVNYFIGVPMGDDPMLNYQCGAFHDGAAPWRITRSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU8</accession>
    <name>Q67MU8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196137.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2010"/>
    <dbReference type="EnsemblBacteria" id="BAD40995">
        <property type="protein sequence ID" value="BAD40995"/>
        <property type="gene ID" value="STH2010"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2010"/>
    <dbReference type="eggNOG" id="COG1768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1183887_0_0_9"/>
    <dbReference type="OMA" id="NHDYWWP"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014578">
        <property type="entry name" value="Pesterase_CT488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033094">
        <property type="entry name" value="Pesterase_CT488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="2"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="28541" checksum="9FD0C17E747B0398" modified="2004-10-11" version="1">MAIYAIGDPHLSLAVNKPMDVFGPRWADHAKRFLQNWEATVGPDDYVLVPGDISWGMTLDEALPDLQEIDRLPGRKLLIQGNHDYWWQSLKKLRELPLRTISFIQNDAVVLPEGSVAGVDGRVAVCGTRGWITPGDRAWDEDPAHNEKIYRREVGRLRLSLEAGRKAGADRYIVMLHYPPVADDHEPTEFTRLLEEWGGVLLCVYGHLHGPSAPHRAYQGLHGTVRYRLVSCDAVDFTPVNLFPQGGDMAGDGGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY1</accession>
    <name>Q67MY1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HflK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1977</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">HflC and HflK could encode or regulate a protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">HflC and HflK may interact to form a multimeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the band 7/mec-2 family. HflK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1977"/>
    <dbReference type="EnsemblBacteria" id="BAD40962">
        <property type="protein sequence ID" value="BAD40962"/>
        <property type="gene ID" value="STH1977"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1977"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039173_0_0_9"/>
    <dbReference type="OMA" id="WNEPGGN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03404">
        <property type="entry name" value="SPFH_HflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010201">
        <property type="entry name" value="HflK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF2">
        <property type="entry name" value="PTHR43327:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01933">
        <property type="entry name" value="hflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PHB" evidence="7">
        <location>
            <begin position="2"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="196"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="279" mass="31034" checksum="81CA055DAD3B738C" modified="2004-10-11" version="1">MEEHESALVLTMGRATRQVDKGVHTKLPWPLETAVVLPTKQTQELQFGFREQNGRVQLVEDEALMITGDENLVWADLLVEWRIQDIEKYLFAVDDPDRLLRNATAAALRSVMGTTGLDFAITTGKFEIQEEVERQLVELMDSYGAGIMIIDVKLQDVEPPQQVSAEFKAVTDAREAQQTKINEAGKYEAERIPAARAEAQKLLEQAEANKQARINQALAEVAQYKAIYEAYKANPDVTRERLLLETLEQILPGADIVIVDSSEGTVKYLPIVPGGGGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N46</accession>
    <name>Q67N46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1912</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196039.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1912"/>
    <dbReference type="EnsemblBacteria" id="BAD40897">
        <property type="protein sequence ID" value="BAD40897"/>
        <property type="gene ID" value="STH1912"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1912"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046987_0_0_9"/>
    <dbReference type="OMA" id="HCTEGHK"/>
    <dbReference type="OrthoDB" id="1456605at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="3">
        <location>
            <begin position="53"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="40835" checksum="1A06BCFFE433B392" modified="2004-10-11" version="1">MTKLAITGATVYPISRPPMAGATLLVGDGKILAVGLVEIPSDAEVVDARGLHLLPGFVDPHTHVGLWGEGDGPPAYDGNEWTSPTTAQVRALDAINPFHVSFADARSAGVTTVQTLPGSGNPIGGEMVVIKTRGRTADEMVLRRPSGLKGALGENPKRLHGQNHKRTPATRMGVAAVIRQFLTRARAYDPEKGRDLGLELARKVLDREIPLRLHAHRADDILTAIRIAREFSIDLSIEHCTEGWMVVDALAEAGYPVTLGPMLGGRSKVETANLTFRNPGILEKAGVHVSLMTDHPFVPIAHHRIQGALAVREGMSEAGALRAMTLSPAEMLGVADRVGSLEPGKDADFVLWSDHPFRARARVLATYIEGQRVYEAKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE9</accession>
    <name>Q67NE9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HlyD_D23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1809"/>
    <dbReference type="EnsemblBacteria" id="BAD40794">
        <property type="protein sequence ID" value="BAD40794"/>
        <property type="gene ID" value="STH1809"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1809"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514738_0_0_9"/>
    <dbReference type="OMA" id="ERMELIG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="187"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="67"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="192"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="529" mass="56703" checksum="48C9AB995429A3E6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH3</accession>
    <name>Q67NH3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YjgF_endoribonc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195913.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1785"/>
    <dbReference type="EnsemblBacteria" id="BAD40770">
        <property type="protein sequence ID" value="BAD40770"/>
        <property type="gene ID" value="STH1785"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1785"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104845_0_1_9"/>
    <dbReference type="OMA" id="VANYVGF"/>
    <dbReference type="OrthoDB" id="1956412at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02199">
        <property type="entry name" value="YjgF_YER057c_UK114_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013813">
        <property type="entry name" value="Endoribo_LPSP/chorism_mut-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14588">
        <property type="entry name" value="YjgF_endoribonc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjgF_endoribonc" evidence="1">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="16032" checksum="A18A32B9AE0B4B28" modified="2004-10-11" version="1">MSVEQRLQELGITIPEAPKPVAAYVPFVRSGDLVFTSGQIAVEGGQLKYKGKVGRDLTAEEGYQAARLCAINTLAVLKSAVGSLDKVAQIVKVVVFVNSADGFTGQPAVANGASELYQQVFGEAGLHARSAVGVSELPMDTAVEVEVIARISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN9</accession>
    <name>Q67NN9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1719"/>
    <dbReference type="EnsemblBacteria" id="BAD40704">
        <property type="protein sequence ID" value="BAD40704"/>
        <property type="gene ID" value="STH1719"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1719"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_650400_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="257"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="359"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="422" mass="45576" checksum="2C5860A0F75A6218" modified="2004-10-11" version="1">MGSVRVKYRGQQAHFREETAMTGAATSGSARVSGIGLLTDLAAFPWHERPPFGWWAAALALIAGRVAQAVDRCRALVTAHPERLDLRARLGVMLLQTGGDSVVQEAVDHLAPAVKRDPDHPHLRRWLAEALVRSGMPEAAIAHLDAGLRVNPGLPDLWVTKGRTLLALGRPEEALVFIDGALAGRPRSGTLWNLRGLCLNELGRLQEAADAYRRAARLLRTDGAVWANLGLALQKTGQTAEALRALLRAVRLRPSDPTLLNNVGYTLLRAGRTREAIESYRQASALEPDSPLTLGNLAAALFEGGMMAEATALLDAALQKAPGDPTLLNNKGHLLLKAGQYREALACFEEALKRAPDDPAALANQAACYFALGRYEQALNAYRLAVATEPHNKQYLWSIGACLERLGRAEEALQTYNQALGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-01-04" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP8</accession>
    <name>Y1710_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0342 protein STH1710</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1710</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0342 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NP8"/>
    <dbReference type="SMR" id="Q67NP8"/>
    <dbReference type="STRING" id="292459.STH1710"/>
    <dbReference type="EnsemblBacteria" id="BAD40695">
        <property type="protein sequence ID" value="BAD40695"/>
        <property type="gene ID" value="STH1710"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1710"/>
    <dbReference type="eggNOG" id="COG3679">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140243_2_0_9"/>
    <dbReference type="OMA" id="YDNANEM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01526">
        <property type="entry name" value="UPF0342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010368">
        <property type="entry name" value="Com_YlbF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023378">
        <property type="entry name" value="YheA/YmcA-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06133">
        <property type="entry name" value="Com_YlbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158622">
        <property type="entry name" value="SSF158622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000110002" description="UPF0342 protein STH1710">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01526"/>
        </source>
    </evidence>
    <sequence length="112" mass="12968" checksum="3641D98AED9C73C3" modified="2004-10-11" version="1">MYDHAHSLARALKESQEYKSFQAAREKIKGKPAAEQMIADFHKRQMELQAEVLQGKELTQEQKEGLERLYNVLIQDLDIRDYLMAEQRLGTLLSDVYKIIGEAVDVDLPFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ2</accession>
    <name>Q67NQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195834.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1706"/>
    <dbReference type="EnsemblBacteria" id="BAD40691">
        <property type="protein sequence ID" value="BAD40691"/>
        <property type="gene ID" value="STH1706"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1706"/>
    <dbReference type="HOGENOM" id="CLU_2703512_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="73" mass="8348" checksum="6B77208347476BEA" modified="2004-10-11" version="1">MNRTSENRLSPTGPRSVPVPWLRYLAPLPLLGFLSLRDPLYGAFFAFGAFLNWGSENRWLRAVSYLGFLGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV1</accession>
    <name>Q67NV1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1657</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CPA3 antiporters (TC 2.A.63) subunit C family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195786.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1657"/>
    <dbReference type="EnsemblBacteria" id="BAD40642">
        <property type="protein sequence ID" value="BAD40642"/>
        <property type="gene ID" value="STH1657"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1657"/>
    <dbReference type="eggNOG" id="COG1006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_3_1_9"/>
    <dbReference type="OMA" id="YRVYEEH"/>
    <dbReference type="OrthoDB" id="2078994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="127" mass="13554" checksum="686354004BF89A54" modified="2004-10-11" version="1">MELLAALVIGLLLGVGTYLLLERNMIRIVLGTMLINYATNFMLLTSGRLKTGRAPFLGGSDVQLAAEGSGGIIVDGISYVDPVPQALILTSIVIGLATTAFAFVLSYRTVQECDTDDLLLLRGVEHE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW2</accession>
    <name>MGSA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylglyoxal synthase</fullName>
            <shortName evidence="1">MGS</shortName>
            <ecNumber evidence="1">4.2.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mgsA</name>
        <name type="ordered locus">STH1646</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dihydroxyacetone phosphate = methylglyoxal + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17937"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="EC" id="4.2.3.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylglyoxal synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195775.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NW2"/>
    <dbReference type="SMR" id="Q67NW2"/>
    <dbReference type="STRING" id="292459.STH1646"/>
    <dbReference type="EnsemblBacteria" id="BAD40631">
        <property type="protein sequence ID" value="BAD40631"/>
        <property type="gene ID" value="STH1646"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1646"/>
    <dbReference type="eggNOG" id="COG1803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120420_1_0_9"/>
    <dbReference type="OMA" id="RACDVHN"/>
    <dbReference type="OrthoDB" id="1726279at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008929">
        <property type="term" value="F:methylglyoxal synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019242">
        <property type="term" value="P:methylglyoxal biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01422">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00549">
        <property type="entry name" value="Methylglyoxal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004363">
        <property type="entry name" value="Methylgl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018148">
        <property type="entry name" value="Methylglyoxal_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30492">
        <property type="entry name" value="PTHR30492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006614">
        <property type="entry name" value="Methylglyox_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00160">
        <property type="entry name" value="MGSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01335">
        <property type="entry name" value="METHYLGLYOXAL_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000178647" description="Methylglyoxal synthase">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="54"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00549"/>
        </source>
    </evidence>
    <sequence length="126" mass="13993" checksum="3343CB51C0FCD6D7" modified="2004-10-11" version="1">MRIALIAHDNRKQDMLKFVKDHKSAFEGHQLFATGTTGKLIREHTGLDVHCFLSGPLGGDQQIGSRVATGEIDMVIFLRDPLTAMPHEPDVQGLLRLCDVRDIPVATNLGSARMFADDLMRLKDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX7</accession>
    <name>Q67NX7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cu_amine_oxidN1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1631"/>
    <dbReference type="EnsemblBacteria" id="BAD40616">
        <property type="protein sequence ID" value="BAD40616"/>
        <property type="gene ID" value="STH1631"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1631"/>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_518664_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="1">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="525" mass="57306" checksum="3E27A1096B14AD83" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P89</accession>
    <name>Q67P89_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nusA</name>
        <name evidence="9" type="ordered locus">STH1519</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195649.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1519"/>
    <dbReference type="EnsemblBacteria" id="BAD40504">
        <property type="protein sequence ID" value="BAD40504"/>
        <property type="gene ID" value="STH1519"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1519"/>
    <dbReference type="eggNOG" id="COG0195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029242_2_2_9"/>
    <dbReference type="OMA" id="MDIDMSA"/>
    <dbReference type="OrthoDB" id="384865at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="135"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="360" mass="40138" checksum="231C9C32DF5EB03F" modified="2004-10-11" version="1">MKAEILEALDDLVRERGISREVLIEAIEAALISAYRRNFGSAQNVRVFFDDKTGEYRVYAQKEIVEEVTDPRLQVSLEEARMKNPSLQLGDVFEVEVTPRDFGRIAAQTAKQVVVQRIREAERGMIYEEYSSREGEIVTGLVQRQDPKTRTVYLELARGVEAVLTANEQIPGERYVENQRIKAYVIEVKRTTKGPAIAVSRTHPGLLKRLFELEVPEIHDGTVEIKGIAREAGSRSKIAVMARDPNVDPVGACVGQKGQRVQNIVDELQGEKIDVVEWSPDPEVFVAKALSPAKVTQVVCDEESRIARVIVPDYQLSLAIGKEGQNARLAAKLTGWKIDIKSESQAEAGAWDELSEDDIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA9</accession>
    <name>DXR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="1">DXP reductoisomerase</shortName>
            <ecNumber evidence="1">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxr</name>
        <name type="ordered locus">STH1499</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DXR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195629.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PA9"/>
    <dbReference type="SMR" id="Q67PA9"/>
    <dbReference type="STRING" id="292459.STH1499"/>
    <dbReference type="EnsemblBacteria" id="BAD40484">
        <property type="protein sequence ID" value="BAD40484"/>
        <property type="gene ID" value="STH1499"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1499"/>
    <dbReference type="eggNOG" id="COG0743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035714_4_0_9"/>
    <dbReference type="OMA" id="PIDSEHF"/>
    <dbReference type="OrthoDB" id="1200722at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00243">
        <property type="entry name" value="Dxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000163716" description="1-deoxy-D-xylulose 5-phosphate reductoisomerase">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <sequence length="387" mass="41527" checksum="20CFCA8161AC1C2C" modified="2004-10-11" version="1">MKKGIAILGSTGSIGTQALDVIDHHPGRFRVVALAAGRQVERLAEQALRFRPALLSVADADAAAALRALLLAEAGDYRPEIAWGREGLIRAATHPESGLVLTSVVGAMGVEPTLAAIEAGKDIALANKETLVAAGELVTAAAQARGVKLLPVDSEHSAIFQSLAGADPGSVRRILLTASGGPFRGRKDLTGVTREEALRHPRWTMGAKVTIDSATLMNKALEMIEAHWLFGVPMEQIEVIVHPQSILHSAVEFCDGNIIAQLGPTDMRLPIQYALCYPERCRGFVEPLDLIALGALTFERPDEETFPSLRYARRAVTMGGTMPAVLNAANEVAVQRFLAGEIPFTGIFQLVAGVMDRHDPVQRPDLAAILAADRWARDAAREQPVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE9</accession>
    <name>Q67PE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195589.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40444">
        <property type="protein sequence ID" value="BAD40444"/>
        <property type="gene ID" value="STH1459"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1459"/>
    <dbReference type="HOGENOM" id="CLU_1184549_0_0_9"/>
    <dbReference type="OrthoDB" id="1201221at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="PFTB_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="26502" checksum="9A1CF5D5ECA386BE" modified="2004-10-11" version="1">MPPDWADRQNPDGGWRSREFSSPVSNMGTTSVTLMRMIWCGLGDSPECRATVEYLRRTQQPDGRWTEDPAQYGANPPEWNRPGDLAVDLWETANNAACLCALGLAQDPVTEKAVAWLRANRREDGTFPGYIHTTYAMAAVCHMRGEGVEAERYLADSLRILHKFKDESWFDVMDLTWALILWGLAGLDVRTEAVRSYRDELRRRRNADGTWSSRYPGCGPQYTLEAVEILRALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI9</accession>
    <name>Q67PI9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="10" type="ordered locus">STH1419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195549.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1419"/>
    <dbReference type="EnsemblBacteria" id="BAD40404">
        <property type="protein sequence ID" value="BAD40404"/>
        <property type="gene ID" value="STH1419"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1419"/>
    <dbReference type="eggNOG" id="COG0128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_1_9"/>
    <dbReference type="OMA" id="CPDIIPV"/>
    <dbReference type="OrthoDB" id="533829at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="449" mass="48530" checksum="0897A9E3A4B1B425" modified="2004-10-11" version="1">MDVRVYPATRLEGTVNPPSSKNYTTRYLLAAALAEGESLIRYPAESEDAAALKRCLADLGAELIPDGPHLRVRGFGANPRNPGTLNPGNAGAVLRFLMAVCAATLDEITFVTDYAESLGRRPHGDLLDALAQLGAQVESREGRLPITIRTGPPAGAGSRSGWERPPSGRVGRLRGGTVRVSGSVSSQYTSALLFAAPLIGEDVTIEVTGDMKSRPPIRQTLQVLQEAGIRVEAGDDLRRFHIPGGQRYRPREYTVPGDYPGAAALMAAAAVVPSDVTIPRLFPDEQGERQAIDVLRAMGADIAHDGQQVWIRGGRPLRGVRFDGDPFTDAVLALTAAAVLAEGETVFYNVENLRYKECDRISDYRAELNKLGARVAEERDKLIVRGRPEGVEGGVTVESRIDHRVIMGLTIVALRAREPVVIRDAHHVAKSYPAFFADLRGLGARVEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ5</accession>
    <name>Q67PQ5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1353</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195483.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1353"/>
    <dbReference type="EnsemblBacteria" id="BAD40338">
        <property type="protein sequence ID" value="BAD40338"/>
        <property type="gene ID" value="STH1353"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1353"/>
    <dbReference type="eggNOG" id="COG0515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_135_2_9"/>
    <dbReference type="OMA" id="DPDYRYQ"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="10"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="10">
        <location>
            <begin position="351"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="10">
        <location>
            <begin position="418"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="10">
        <location>
            <begin position="485"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="630" mass="68119" checksum="5DAF6F3CC700B7FC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR3</accession>
    <name>Q67PR3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Zn-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1345</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1345"/>
    <dbReference type="EnsemblBacteria" id="BAD40330">
        <property type="protein sequence ID" value="BAD40330"/>
        <property type="gene ID" value="STH1345"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1345"/>
    <dbReference type="eggNOG" id="COG2738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084406_0_0_9"/>
    <dbReference type="OMA" id="VQHATAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007395">
        <property type="entry name" value="Zn_peptidase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36434">
        <property type="entry name" value="PTHR36434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04298">
        <property type="entry name" value="Zn_peptidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24403" checksum="7E53B16E3B6C708C" modified="2004-10-11" version="1">MIFLLPAIILVIWAQARVRSSFNEWSQVGTRSGVTAAQVARDILDRHGLTDVPVERVRGYLSDHYDPQKRVVRLSDSTYSSNSIAAIGVAAHEVGHAIQHELSYTPLQVRNLIWPVARIGDSLGPFLVIIGLIFGGYSGQMLMDIGILLFLGAVLFYLITLPVEFNASSRAVEILETGGYMTREEVAGARKVLNAAALTYVAGAATAIMSLVRLLFLRSMASDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU3</accession>
    <name>Q67PU3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1315</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195446.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1315"/>
    <dbReference type="EnsemblBacteria" id="BAD40300">
        <property type="protein sequence ID" value="BAD40300"/>
        <property type="gene ID" value="STH1315"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1315"/>
    <dbReference type="eggNOG" id="COG2152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046648_0_0_9"/>
    <dbReference type="OMA" id="WLVIYHA"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="33711" checksum="1E135EC346DE9F7C" modified="2004-10-11" version="1">MIKLTRLATKPILKPNPANEWERAAVFNTAAIYDNGLFHLLYRATNIGGHARYGRYMNAIGYAVSCDGIHFTRLDRPVLAPGEHVQELRGAEDGRLVKIGDTYHLTYTGFSDRFEGDYRICRATSKNLITWERHGPMLDEPNKNASLFPEKVGGRYVLLHRRYPDIWIAYSDDLVTWTDHRKILSPIEGTWQDARVGIAGPPIRTEQGWVLIYHGARKADNSYRLGIALLDAEDPSVVLARQDEPIIEPELEWEREGFVPNVIFSTGHALRGDDLYVYYGGADTVIGVAAVRLSDLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW4</accession>
    <name>Q67PW4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1294</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1294"/>
    <dbReference type="EnsemblBacteria" id="BAD40279">
        <property type="protein sequence ID" value="BAD40279"/>
        <property type="gene ID" value="STH1294"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1294"/>
    <dbReference type="eggNOG" id="ENOG502ZBVQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_737553_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269319" evidence="2">
        <location>
            <begin position="27"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="375" mass="42170" checksum="176E1055D79109A0" modified="2004-10-11" version="1">MGVRARLVSGMLALLLLIGGCGQVDAAGLVEEYLTALTIGDFDRAAQLSTEALVELNGDDSAEARFVQTYLAQVRFTVREAQKEDDDRYRVPVELIAPDLRPIVGRVLANLLGEAFASAFMLTPLDEKTMTQRLVDSLTAEITRSDASTITVERTVMVERIDGQWKVTTPVFAESGLLDIFDDGFLANSYAEESRARRAESLRRRIAAIEEQLTELRRDREFAEQSRSKLSKFVVSNAKFYWAVDGWWEEPVIELTVQNNTEFPVARAYFHGRLVSPGRSVPWVEEDFNYAIPGGIEPGEIQTWKLVANSFSTWGSAPRDRDDLILEVEVTRLDGPNDQVLLDAEFPDYKAEWLADLEADLAELQEELRALRTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX4</accession>
    <name>Q67PX4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1284</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195415.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1284"/>
    <dbReference type="EnsemblBacteria" id="BAD40269">
        <property type="protein sequence ID" value="BAD40269"/>
        <property type="gene ID" value="STH1284"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1284"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_1_1_9"/>
    <dbReference type="OMA" id="TQYVVYA"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="35374" checksum="630442A3ACF45450" modified="2004-10-11" version="1">MSARRLRLVQAGPFAFRVDPGMVARVAGAWLALLALGAWGLTLGSYRLPVIDVVRSLLGAGTADTQFIVMTLRLPRVLAAMLVGPALAMSGAIFQGLLRNPLVSPDVIGVNAGASVAAVYWIVTHRPPALLPLIAFAGALAAAAFVYLLTWRGHISGARLILVGIGANALLTAATTAIFLRADVYEAASAYRWMTGSLAGSDWSDVITLAVALAVLVPPGLVLMWPLRALQYGDLTARSLGLPVEGVRLALMAVGCGLSAAAVSVAGPVGFVAFMVPHLARMLAGPPGGGLFLFTGALGGLLLLGADLVGQHALPVSLPVGVITAALGAPYFLFLLYRTNARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q29</accession>
    <name>Q67Q29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195360.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1229"/>
    <dbReference type="EnsemblBacteria" id="BAD40214">
        <property type="protein sequence ID" value="BAD40214"/>
        <property type="gene ID" value="STH1229"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1229"/>
    <dbReference type="eggNOG" id="COG0762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136788_0_1_9"/>
    <dbReference type="OrthoDB" id="2069856at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="11390" checksum="178F08DFC7C8AB6D" modified="2004-10-11" version="1">MQFLIYQTVSIFLSILEVLIWARVLMSWFRPRYRTSSNSWFFTIEDYVWRATEPMLAPIRNILPDMGMIDFSPFVLMILLALVRRLVGMLIFGPLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q57</accession>
    <name>RSMH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmH</name>
        <name type="synonym">mraW</name>
        <name type="ordered locus">STH1201</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195332.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q57"/>
    <dbReference type="SMR" id="Q67Q57"/>
    <dbReference type="STRING" id="292459.STH1201"/>
    <dbReference type="EnsemblBacteria" id="BAD40186">
        <property type="protein sequence ID" value="BAD40186"/>
        <property type="gene ID" value="STH1201"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1201"/>
    <dbReference type="eggNOG" id="COG0275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038422_2_0_9"/>
    <dbReference type="OMA" id="NPAKRTF"/>
    <dbReference type="OrthoDB" id="1272633at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000108727" description="Ribosomal RNA small subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <sequence length="318" mass="34830" checksum="A59D0BB24EA0ABE9" modified="2004-10-11" version="1">MAFHHIPVLLQETMEALAVRPGGTYIDCTVGGGGHAAEILRRSSPDGRLIGLDQDENALRAAGDRLAPFGDRVTLVRTNFEHVADVADRLGLGAADGVLMDIGVSSHQFDEGERGFSYHHDAPLDMRMDRTRPLTAAVLVNEWEEEEIARVIREYGEERWASRIAQFIVRARRQRPIETTGQLVEIIKAAIPASARREGGHPARRTFQAIRIAVNDELGALERGLEGALRVLRPGGRLAVITFHSLEDRIVKQTFARWAKPCTCPPDLPVCVCGKAPLAEPVTRKPVRASGAEVKANPRSRSATLRAVVKLQAGEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q75</accession>
    <name>Q67Q75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1183"/>
    <dbReference type="EnsemblBacteria" id="BAD40168">
        <property type="protein sequence ID" value="BAD40168"/>
        <property type="gene ID" value="STH1183"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1183"/>
    <dbReference type="HOGENOM" id="CLU_1395690_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="20006" checksum="4E8980A56F9BB995" modified="2004-10-11" version="1">MGVTFALFTDSREGGSHELVAGTVRIDGERVNDTVRGPMFYIDGASDGVTHDGQEGLLPTGLWAPGDAHHRGFQIENVGTLDVKLTGMTAELKAGDEVLAGALDVKIYDDLYLDEDGHVLNPEATLIYSGKLAEFLGSGMSFPTPIELAPGDLASIGILVSFPLDAGDAYQGTTIKVTFSAHAEQLRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB0</accession>
    <name>Q67QB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acyl-L-amino acid amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1148"/>
    <dbReference type="EnsemblBacteria" id="BAD40133">
        <property type="protein sequence ID" value="BAD40133"/>
        <property type="gene ID" value="STH1148"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1148"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_9"/>
    <dbReference type="OMA" id="PILMASC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="187"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="392" mass="42346" checksum="A855272D8199DF4B" modified="2004-10-11" version="1">MWALARSVEEYGIAVRRRLHRNPELSFAEHDTHQYLAEELQGLGCSFRSHLAGGTGLHVVLGGTRPGPVVALRADIDALPIQEETGLPFASERPGVMHACGHDVHTAILLATARALKSVEQDLPGTVVLLFQPGEEKNPGGASLMIRDGVLDQPKVDAIFGLHVDPYLEAGRMAFASGPVMAAPDELRVTVTGRGGHGAWPHQTVDPVVTAAQIITLLQQVVARNVDPFQPAVLTVGMIHGGTAHNIIPDEVEFIGTVRTMDEGLRRRMPERIEAVIRGVCEAAGASYRMEYERGYPVLVNHPEATETGRRAAAAVLGEDRVGRMEPSMGGEDFAYYLERVPGTFARLGARSPGDAAPHGLHTSRLMIDESCIAVGVAYYIQVVQQFLMGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC8</accession>
    <name>Q67QC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transposase IS21/IS408/IS1162 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1130"/>
    <dbReference type="EnsemblBacteria" id="BAD40115">
        <property type="protein sequence ID" value="BAD40115"/>
        <property type="gene ID" value="STH1130"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1130"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_1_1_9"/>
    <dbReference type="OMA" id="CMRTENG"/>
    <dbReference type="OrthoDB" id="362657at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017894">
        <property type="entry name" value="HTH_IS21_transposase_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50531">
        <property type="entry name" value="HTH_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0814">Transposable element</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="HTH IS21-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="7">
        <location>
            <begin position="110"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="408" mass="47334" checksum="60A5185E0197C2DC" modified="2004-10-11" version="1">MLRSGETLEIRQMYAGGLSISEIARRTGRDRKTIRKWLRTNTMPKPAKRKRSSMLDQHEAFTLEQMQKGVTSASKMLYLLQQRGFKGKIRIVRAFMAPYRPMAKAAATVRFETPPGKQAQVDWADFGYIEVDGRRLKLYCFIMVLAYSRAMYLEFVTATDMKTFMRCHINAFKFFGGVPHEILYDNVKTVVKDRDDDNRPVFNERFLDFSSYYGFRPRLCLPYHAWTKGKVERPVRYIRQNFWQGIQFCSLEDLNRQAALWRDTVANVRIHATTRVQPVVRLAEEDLIPLTNRVDFDTSEYSSRRVSREVAISYKGNRYSVPWQLVGKDVVVRLLPSGQEIQVLWQGQIVAQHELAEGKGKFVVDPAHFQGLNRTKTARPVLVLRKPPGHDEVEQRDLAYYDAFAGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD4</accession>
    <name>Q67QD4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1124</name>
        <name evidence="2" type="ordered locus">STH744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">IAM14863</strain>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194877.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1124"/>
    <dbReference type="EnsemblBacteria" id="BAD39729">
        <property type="protein sequence ID" value="BAD39729"/>
        <property type="gene ID" value="STH744"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40109">
        <property type="protein sequence ID" value="BAD40109"/>
        <property type="gene ID" value="STH1124"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1124"/>
    <dbReference type="KEGG" id="sth:STH744"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="SRSIAME"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49943" checksum="8E6A3AD284E4720B" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRCLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF6</accession>
    <name>Q67QF6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable lipid II flippase MurJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">murJ</name>
        <name evidence="10" type="ordered locus">STH1102</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the MurJ/MviN family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195234.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1102"/>
    <dbReference type="EnsemblBacteria" id="BAD40087">
        <property type="protein sequence ID" value="BAD40087"/>
        <property type="gene ID" value="STH1102"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1102"/>
    <dbReference type="eggNOG" id="COG0728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006797_4_1_9"/>
    <dbReference type="OMA" id="IFFVAFK"/>
    <dbReference type="OrthoDB" id="749401at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015648">
        <property type="term" value="F:lipid-linked peptidoglycan transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13123">
        <property type="entry name" value="MATE_MurJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02078">
        <property type="entry name" value="MurJ_MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004268">
        <property type="entry name" value="MurJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03023">
        <property type="entry name" value="MurJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002869">
        <property type="entry name" value="MviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01806">
        <property type="entry name" value="VIRFACTRMVIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01695">
        <property type="entry name" value="murJ_mviN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="522" mass="55903" checksum="9FC0DBB3A936655D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH3</accession>
    <name>Q67QH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1085</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195217.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1085"/>
    <dbReference type="EnsemblBacteria" id="BAD40070">
        <property type="protein sequence ID" value="BAD40070"/>
        <property type="gene ID" value="STH1085"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1085"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_9"/>
    <dbReference type="OMA" id="MYVDQSR"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="300"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="333"/>
            <end position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="561" mass="62723" checksum="354F9BAC2FD1C52B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI7</accession>
    <name>Q67QI7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1,4-dihydroxy-2-naphthoate octaprenyltransferase</fullName>
            <shortName evidence="6">DHNA-octaprenyltransferase</shortName>
            <ecNumber evidence="6">2.5.1.74</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">menA</name>
        <name evidence="7" type="ordered locus">STH1071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to demethylmenaquinone (DMK).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,4-dihydroxy-2-naphthoate + an all-trans-polyprenyl diphosphate + H(+) = a 2-demethylmenaquinol + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26478"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:11173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="EC" id="2.5.1.74"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MenA family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.74" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195203.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1071"/>
    <dbReference type="EnsemblBacteria" id="BAD40056">
        <property type="protein sequence ID" value="BAD40056"/>
        <property type="gene ID" value="STH1071"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1071"/>
    <dbReference type="eggNOG" id="COG1575">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043611_3_0_9"/>
    <dbReference type="OMA" id="QWIEGAR"/>
    <dbReference type="OrthoDB" id="1472516at2"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00168"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046428">
        <property type="term" value="F:1,4-dihydroxy-2-naphthoate octaprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01937">
        <property type="entry name" value="MenA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004657">
        <property type="entry name" value="MenA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="PTHR13929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="31822" checksum="E0F1C1E058864D2C" modified="2004-10-11" version="1">MAVRARFGVWWRMLRPFTLVASVAPVLGGTALAYAHGGFHTGRFLAFLLAAVLIQSATNMFNEYFDYVRGLDTRDHVGIAGTIVRDGVSPRVVITLAWMFLATAVALGLYISATSSWWVFAVGLACLGVAFLYSGGPLPLSSTPFGELAAGFTMGPVMILLVYYTQTLRVTATALWVAVPIGILIGAILLANNIRDIEADRAGGRHTLPIVLGRGAAVGVLAGSFVLAYAIAVGLVATGRLTPWALLILLSLPKAWPPVRLFLREREPVRLHPAVKGVAQLLAGFGGLMTLGLLLSVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN8</accession>
    <name>Q67QN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1020"/>
    <dbReference type="EnsemblBacteria" id="BAD40005">
        <property type="protein sequence ID" value="BAD40005"/>
        <property type="gene ID" value="STH1020"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1020"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2071964_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="118" mass="12895" checksum="464CB58B06BA4719" modified="2004-10-11" version="1">MRESRPARRISDQAVMAVFTFTPQAAQKLKQIIQERGGNLALSIAADPEVTGGAWRLTLVPRRPSTAVVSGVPVEADAEALRLLDGTIIDWVMTPDGPGLGIYDQNLVDNDPRRRGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP3</accession>
    <name>Q67QP3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195147.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1015"/>
    <dbReference type="EnsemblBacteria" id="BAD40000">
        <property type="protein sequence ID" value="BAD40000"/>
        <property type="gene ID" value="STH1015"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1015"/>
    <dbReference type="HOGENOM" id="CLU_2221882_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="11058" checksum="48A3E4D8D87F9727" modified="2004-10-11" version="1">MNELQLLFQALASLLPEAILSRVLTYAAITAALVALLRRIPWFEARRQVAAPVAAVVLGQGFGWLAVGFDTAQWGTAVGAGVVIALAAVGGYSGTKNVAQHVRKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP8</accession>
    <name>Q67QP8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chromate efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1010"/>
    <dbReference type="EnsemblBacteria" id="BAD39995">
        <property type="protein sequence ID" value="BAD39995"/>
        <property type="gene ID" value="STH1010"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1010"/>
    <dbReference type="eggNOG" id="COG2059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_1_2_9"/>
    <dbReference type="OMA" id="MLGIMAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="183" mass="19623" checksum="D900F2A10ED7C6FB" modified="2004-10-11" version="1">MQWKTLLDLFIGFGRATLLGFGGGPSIVPLYEHEAVEVFAWVSREEFGTALAMGNALPGPIATKLTMYIGYRVAGWPGAIVALLAVTLPVGLIMIALAGLMLRFKDSPVLKGMVNGIRPVVFVMLAMLAWDFAGYAFQPKGTGLLSWLPFGIAAAYFISVQYLKLSPIYGVVASLVIGALLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR0</accession>
    <name>Q67QR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdenum ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH998</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195130.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH998"/>
    <dbReference type="EnsemblBacteria" id="BAD39983">
        <property type="protein sequence ID" value="BAD39983"/>
        <property type="gene ID" value="STH998"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH998"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="FVSHNRD"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="39276" checksum="F3F7B81F5B8EA81D" modified="2004-10-11" version="1">MLTCRIEKRLGAFHLQVDLTAGPHITALFGPSGSGKSLTLQAIAGLVRPDRGVIRLNGRTLFDGEAGTNLPPQARRVGYVPQHYALFPHMTVLQNVAYGLHRLPRRERRERAAAAIAAVGLAGLESRRPGELSGGQQQRVALARALVTRPEVLLLDEPFAALDDATRRALHGELLAVLAEQQIPTLLVTHQLEEAYALSRTIAVYEAGRVVQLGPRTAVFQRPATLEAAAQMGFHNRLEGVVAAVDGEGARVRVGPHQLLAPAGDARPGERVVCAIRPEHVMLVRKDRPLRAPGDTVLAGEIVEEIDYGGDVLLLFRSDPPGVELQVSLPGYVYERLHVAEEKRWDVVLRRAYLRTYRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT1</accession>
    <name>Q67QT1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH977</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195109.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH977"/>
    <dbReference type="EnsemblBacteria" id="BAD39962">
        <property type="protein sequence ID" value="BAD39962"/>
        <property type="gene ID" value="STH977"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH977"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_9"/>
    <dbReference type="OMA" id="FYRHQTQ"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="306"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="42170" checksum="3E13217C45E310D0" modified="2004-10-11" version="1">MNLLETLRVAWEALVANKMRSILTMIGIIIGVGSVIAVVAIGRGTESAVLGELQGLGAGVFYIMPGSVSSSDALSRIEPISERDMQQLEAMLPDVEAVISGDTVGVRAEYQKETLSTYAYAVSPGFDRVVNVKVLEGRWFTEEEMKGGARVTVLSRDAVTRLFGDRDYNPVGERVKINGLTHQVIGTVENSTGALSQAMGVQDPAVYLPVAAVQRMTGSTDSFLVLAKVRPGADVESVMNDAVALLGQLNNGAKYMGYSMEQMTAALSSVTGLLTGLIASIAAISLLVGGVGIMNIMLVSVTERTREIGLRKAIGATYGNIMTQFLIESVVICLVGGAVGVAFATVPVWLVGRAMGVSMLIDLPTVLLAMGFSAAVGVLFGVYPASKAARLDPIEALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU6</accession>
    <name>Q67QU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH962"/>
    <dbReference type="EnsemblBacteria" id="BAD39947">
        <property type="protein sequence ID" value="BAD39947"/>
        <property type="gene ID" value="STH962"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH962"/>
    <dbReference type="HOGENOM" id="CLU_1874383_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="14442" checksum="46488142D45263F6" modified="2004-10-11" version="1">MLPTSARRARRAHFASYPSQLPECRRRRRPTLRPVPARLHTSLVRGSFRMAKGTQLSGNNITGVSGAQHGAKQNAASMQRAAGKAGQANYEFGSMGTGQQSGAQLSGNNITGVSGAQHAAKRNNASMQRAAQKADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R33</accession>
    <name>Q67R33_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195007.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH875"/>
    <dbReference type="EnsemblBacteria" id="BAD39860">
        <property type="protein sequence ID" value="BAD39860"/>
        <property type="gene ID" value="STH875"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH875"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_9"/>
    <dbReference type="OMA" id="LWQQGWL"/>
    <dbReference type="OrthoDB" id="1531811at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="55"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27301" checksum="512378AD587EA532" modified="2004-10-11" version="1">MARWKAGLISAGLLLVAWQALCWTGLWPRYVFPWPEDVALTLWRLLTSLELAVALTHTLKRIGLGFALSAVGGLLLGLAMARSRTLSDLLGPVVQGLQSMPSICWYPLAVLWFGWNEGALLFVTTAGALFAVAAATESGVRNIPPSYVRAATTMGARGWRLYTRVVLPAALPSLLTGLRLGWSFAWRSLMAGELLFLNLGFGHLLAMGRDLGDASQVMAVIVVILAVGMVVDRACFSRAEAAVRVRFGYDKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R67</accession>
    <name>Q67R67_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194974.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39826">
        <property type="protein sequence ID" value="BAD39826"/>
        <property type="gene ID" value="STH841"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH841"/>
    <dbReference type="eggNOG" id="COG2013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040551_4_1_9"/>
    <dbReference type="OMA" id="AMVYMKG"/>
    <dbReference type="OrthoDB" id="1063432at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00266">
        <property type="entry name" value="TIGR00266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="226" mass="24106" checksum="E9B9AFCC250DBBD1" modified="2004-10-11" version="1">MRCEIRYQPSYSLAVVYLGPGEEVQAEPGAMVAMSSTVELETEVKGGFLGALGRSVLGGESFFTSRYRARGGPGELALAPTLPGDIGYVELRGETFYLKSGAYLAADPELAVDSRWGGARGFFGSGGLFLLRVQGTGGLLFTAYGALHEKELAPGERYRVDTGHVVGFSEGLNFAVRKAARGWFSTLASGEGLVCEFTGPGRVYLQTRSEEALVNWMVAQLPSKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R68</accession>
    <name>Q67R68_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH840"/>
    <dbReference type="EnsemblBacteria" id="BAD39825">
        <property type="protein sequence ID" value="BAD39825"/>
        <property type="gene ID" value="STH840"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH840"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_0_9"/>
    <dbReference type="OMA" id="NMAYFTP"/>
    <dbReference type="OrthoDB" id="1413173at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="PTHR42961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="6">
        <location>
            <begin position="38"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="155"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="42927" checksum="D5EF0DC62061C551" modified="2004-10-11" version="1">MTGITPQRVESGYAVGSCVDESPVSGPGRRANRMAVTREQVLDALKVVNDPELHKSIVDLDMVKDIQIDGGHVAVTINLTVPGCPLKHRFERDVEAALKQVPGVERVTTHFGAMTDAERAAVAAKVRGSSAPHADARPSTMALATRTTIIGVASGKGGVGKSTTTVNLAVALKKLGYSVGIIDADIYGFSIPRMMGNMSRPEALDDQMLLPVWAHDIPFISAGSLVNEDQAIIWRGPMLGKMVEQFLVNVQWGKLDYLLIDLPPGTGDVALSVAQMLPGTDLVLVTTPQAAASQVAARVGSMAARTKQRVVGVIENMAYFLCDECNKKHFIFGKGGGQALAASLGCEVLSQIPLTPDVRLGSDVGDPIVADKEDHPAAQAYLEAARKLAQLAPPKVWSDELRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE8</accession>
    <name>Q67RE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194893.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH760"/>
    <dbReference type="EnsemblBacteria" id="BAD39745">
        <property type="protein sequence ID" value="BAD39745"/>
        <property type="gene ID" value="STH760"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH760"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062584_0_0_9"/>
    <dbReference type="OMA" id="ASLNEQW"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027024">
        <property type="entry name" value="UCP027386_ABC_sbc_TM0202"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027386">
        <property type="entry name" value="UCP027386_ABC_sbc_TM0202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269505" evidence="2">
        <location>
            <begin position="28"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="NMT1" evidence="3">
        <location>
            <begin position="125"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="358" mass="38862" checksum="B88F894CC272DAEC" modified="2004-10-11" version="1">MRPFKSTFPKLIACFLALGLLCGCAGRAPENPPARQPEQTPAAEQTPAAEQAPQAEPLTALHLQAPSAPPSILLAHLASQERLTSLVPEVQFQAFQNLDQLRADMVKGAVQVAAVPTYVAANLYNKGLPVRLLNVTVWGHLSVLTADPDIQSWEDLKGKTVYIPFKNDSPDLIFRTVAKLAGVPEEEMGLQYVSAAPEALQMLMAGKADAAVLPEPVATAAILQGKQNNIPVRELFELRQEWGRVTGREPFIPQIGTIAVADLVDQHPEVIAAVQEGLKESVQWIQENPADAAALGEPHLSGLKAPVIQNSLDRTPFRFQTATEAREAVEFYFTKLAELNPEIIGGRLPDDGFYYTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF5</accession>
    <name>Q67RF5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH753"/>
    <dbReference type="EnsemblBacteria" id="BAD39738">
        <property type="protein sequence ID" value="BAD39738"/>
        <property type="gene ID" value="STH753"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH753"/>
    <dbReference type="HOGENOM" id="CLU_1165332_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="26243" checksum="F9246D137CAA074C" modified="2004-10-11" version="1">MAFPGRDLRLRRLSALTLVLLLAAAALAGCVRLELNLRVNPDGTGEKEIIAAIDREFLAVAAAMRARDPLARLEAELREDPKATVTRYQLGDMVGFRAVAPFRNELILNDETWKGHFLIRDRIFWRDYTLDLETLLDMAELDAVAASFDSPVDFRVSVELPARIGETNGELDPSGRKVTWTLIPGRRDHLVLTARQYLYGRIAAAGAAALAAAVGLAFGIRRGQRRRRPVGGIGQAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR3</accession>
    <name>Q67RR3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proline dehydrogenase</fullName>
            <ecNumber evidence="3">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH645</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + L-proline = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH645"/>
    <dbReference type="EnsemblBacteria" id="BAD39630">
        <property type="protein sequence ID" value="BAD39630"/>
        <property type="gene ID" value="STH645"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH645"/>
    <dbReference type="eggNOG" id="COG0506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061158_0_0_9"/>
    <dbReference type="OMA" id="YPMIGSH"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00373"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:proline catabolic process to glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008219">
        <property type="entry name" value="PRODH_bac_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PTHR13914"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000196">
        <property type="entry name" value="Pro_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pro_dh" evidence="8">
        <location>
            <begin position="47"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="224"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000196-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000196-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="33987" checksum="B524A918CE5C67B9" modified="2004-10-11" version="1">MSSLARKAILSLAGNRFVTRVMTRHGLKIGAGRFVAGVTLQDAVAVTRRLNDEGLAVTLDLLGEGVTDRDGARAMAEGCAGILEAIGREKLDANLSVKLTQLGLAVDPEAAMENMVYLQDIARQAGIFIRIDMESSDVTDVTLDIVRRLYARERNVGTVIQAYLYRSPKDLEELARLGMNVRLVKGAYLEPPSVAYPKKADVDAAFRRLIAQHLSAGCYTAVATHDDAIIAFTESFVKEKGIPRDQFEFQMLYGIRPERQRELARAGYRTRVYVPFGSDWYPYFVRRLAERPANVWFVLANLMKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT8</accession>
    <name>Q67RT8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194753.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH620"/>
    <dbReference type="EnsemblBacteria" id="BAD39605">
        <property type="protein sequence ID" value="BAD39605"/>
        <property type="gene ID" value="STH620"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH620"/>
    <dbReference type="eggNOG" id="COG3255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105945_1_4_9"/>
    <dbReference type="OMA" id="DSTCELQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039543">
        <property type="entry name" value="POX18/UbiT/NSL-TP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="PTHR10094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="1">
        <location>
            <begin position="7"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="11530" checksum="CE33E01E25A3EFE8" modified="2004-10-11" version="1">MTIAELLEKLKAQIAANPAKIANLKATYQFELTGEGGGTYHAVFDNGQYKIGEGPVENPGCTITMATSDFFALMERKLNPMAAFMSGKLKIRGDMGQAMKLHQLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S23</accession>
    <name>Q67S23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH-dependent butanol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194669.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH535"/>
    <dbReference type="EnsemblBacteria" id="BAD39520">
        <property type="protein sequence ID" value="BAD39520"/>
        <property type="gene ID" value="STH535"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH535"/>
    <dbReference type="eggNOG" id="COG1979">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_4_9"/>
    <dbReference type="OMA" id="CTMALNG"/>
    <dbReference type="OrthoDB" id="717704at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990362">
        <property type="term" value="F:butanol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08187">
        <property type="entry name" value="BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044731">
        <property type="entry name" value="BDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43633">
        <property type="entry name" value="PTHR43633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="3">
        <location>
            <begin position="9"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="390" mass="42918" checksum="9192AA2B58B838B2" modified="2004-10-11" version="1">MENFVFHNPTRLIFGRGQLSRLRDELEPYGKRVLLVYGGGSIKRTGLYDEVMAILREAGRTVFELPGVEPNPRLTTVHRGVEICRREQIDFILAVGGGSVLDCAKAIAVGARYDGDMWDIVTRKAEATGALPFGAVLTLAATGSEMNPTSVITNWETQQKLGWSARPYTFPAFSILDPVYTFTVPRDHTVYGIVDMMSHALEQYFHAADNAPLIDRWIEDLLRTVMEAAPKAVANPEDYEARETLLFCGTMALNGVLSMGTDGGDWATHAIEHAISAVYDIPHGGGLAIVQPNWMKYCLETDPARFARLAVRVFDVAPAGRTDREVGLEGIERLRQFWSSLGAPARLADYGIGDDQIDRMAVLAMKGGTTVGRFRPLTPEDVAAILRMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S37</accession>
    <name>Q67S37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH521"/>
    <dbReference type="EnsemblBacteria" id="BAD39506">
        <property type="protein sequence ID" value="BAD39506"/>
        <property type="gene ID" value="STH521"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH521"/>
    <dbReference type="HOGENOM" id="CLU_1401833_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="19814" checksum="D87A08C66103047C" modified="2004-10-11" version="1">MILGGGWAKVKERLEGGSPSSRRAARRRHELPDAGLPDRPGRSGRGPAASDVERRLPAPAGAGRPPAPEVARTEAAGMEGFLTEGVRTEGFGTEGLQTEGLRQEGFESEGVGSEGVPREGLRPTVAPSALRSSLQRSAGGDAGGTARAPTGADAGPAVDPGEAAALLSTREGLARAVLLAEVLGKPRALRPYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S43</accession>
    <name>Q67S43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194649.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH515"/>
    <dbReference type="EnsemblBacteria" id="BAD39500">
        <property type="protein sequence ID" value="BAD39500"/>
        <property type="gene ID" value="STH515"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH515"/>
    <dbReference type="eggNOG" id="COG1610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079430_2_1_9"/>
    <dbReference type="OMA" id="AMGAVMK"/>
    <dbReference type="OrthoDB" id="1498741at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="PTHR28055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="16405" checksum="202245598B5F1637" modified="2004-10-11" version="1">MNLNERLTEDMKAALKAGPAGKARLETIRFLRAAVKNAEIEKHAPLSDDEILGIITKQVKQLRESLADFQRSGRQDLVEKTEAEIAVLSEYLPEQLSADEVRDLARKVIAQVGAQGARDMGKVMGPLMAQVRGRADGKMVQQIVKELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S83</accession>
    <name>Q67S83_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA_pol3_delta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194609.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH475"/>
    <dbReference type="EnsemblBacteria" id="BAD39460">
        <property type="protein sequence ID" value="BAD39460"/>
        <property type="gene ID" value="STH475"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH475"/>
    <dbReference type="eggNOG" id="COG1466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044694_2_1_9"/>
    <dbReference type="OMA" id="EEPYYID"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010372">
        <property type="entry name" value="DNA_pol3_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005790">
        <property type="entry name" value="DNA_polIII_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06144">
        <property type="entry name" value="DNA_pol3_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01128">
        <property type="entry name" value="holA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_pol3_delta" evidence="1">
        <location>
            <begin position="19"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="344" mass="37698" checksum="103E587CD6C4BA49" modified="2004-10-11" version="1">MQYHEVLDEIGRGILRPVYLVYGEEGFLVEEVYRALRAAVVHPETADFNFHVLEPGPDQLSQALSLAQTQPFFAEKRLVVVKECPAIVPRRRGGSDEQAPADAGDAALLGYLKAPVASTVLLFLAAQVDVRRKTTKALMAAGAAVECRPLKPEDAAMWAQRRAQSEGKRLGGQAAHLLVERVGTDLGMLARELEKLVLYVGPAGEIRAADVETMVSAMAETEIFRLSDAVLRRERARAVALLERLLQQVDHPLQLLSALTGRFRQLLLVKALAARRLSPKEAAQLAHMHPYAYGKLAEHAATVDRAEIVRALKRLLEADLAIKSGYDPRLTLETVVAELVGEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S91</accession>
    <name>Q67S91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194601.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH467"/>
    <dbReference type="EnsemblBacteria" id="BAD39452">
        <property type="protein sequence ID" value="BAD39452"/>
        <property type="gene ID" value="STH467"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH467"/>
    <dbReference type="eggNOG" id="ENOG5032WB6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_815528_0_0_9"/>
    <dbReference type="OMA" id="FDIINAY"/>
    <dbReference type="OrthoDB" id="2047341at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="32884" checksum="8A665602DFF128AA" modified="2004-10-11" version="1">MSTHSPAPADDVLRPRDLGQILDQTFRVFGRSWRSLLPMGLIGATPGLLYSLFVLSLSPDLQMGPFGNPLVRALAAADAGDFSGVLRLGSLMVVSWVAILLLYPLYKGALLDAATRAVLHMPPVSVGESFRVGATRYGAMLGSHALLVLMWIAAVPLLALAGLLVLAFLTIPVGLIALATFTVFTGHAVVVEQKGAGSAIGRSFELVRSRFWPLLGTGIVFWLLSTLLSYIVVGPSSFAAGIVTAITGSFLPFTLLTLVEGLAATFITPFMAVGLTVVYFDTRVRREGYDLEWMARQQAESATGDFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S95</accession>
    <name>Q67S95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanol dehydrogenase regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">moxR3</name>
        <name evidence="2" type="ordered locus">STH463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194597.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH463"/>
    <dbReference type="EnsemblBacteria" id="BAD39448">
        <property type="protein sequence ID" value="BAD39448"/>
        <property type="gene ID" value="STH463"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH463"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_9"/>
    <dbReference type="OMA" id="RHRISTN"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="37"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="35380" checksum="38FA5F2417828371" modified="2004-10-11" version="1">MPLTETVETLRRIEAELRKVIVGQDQVIEQLLVALLAGGHVLIEGVPGLAKTLLVRSLAQALGVSFRRIQFTPDLMPADVVGTTVFNPRDLTFEVRRGPVFTNLLLADEVNRTPPKTQSALLEAMEERQVTLDGQPYPLPEPFMVIATQNPIEYEGTYPLPEAQLDRFLMKIQMDYPAAEEERAILERHYRGFRAQELDRAGIGRVLDGPGLLALRSAHRSVTVEPEVLDYINRIVRATRAWSTLAVGASPRGAVALLVCAQSLALIRGRDYVVPDDIKEMAAPVLRHRVILRPEAEVEGVRADQVLEQILQSQPVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC0</accession>
    <name>Q67SC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sigma-54-dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH438</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH438"/>
    <dbReference type="EnsemblBacteria" id="BAD39423">
        <property type="protein sequence ID" value="BAD39423"/>
        <property type="gene ID" value="STH438"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH438"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_1_9"/>
    <dbReference type="OMA" id="YYRLHIV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="31"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="161"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="479" mass="52266" checksum="1148B2433A03E2DE" modified="2004-10-11" version="1">MEVTTRRHGKSAAHRRGGAHVDGAILRYAIQVLDSLHDGVVAVDSEGYVIYANEANTRITGLTRDQVVGRHVSEVVPNSHILDVLASRTPLLGVRTRVFDHVVVSNIVPVYHQGRMVGAVSVFRDITEVLALSRQLDEARNTIDLLRENLTAAPVAEDGVIIGQSPAAQRMYLTARKAATVDSPVLIEGESGTGKEVMARLIHNRSARRSQPLIAVNCAAIPGTLLESELFGYEEGAFTGSRKGGRPGLFQLADGGTLFLDEIGDMDPGLQAKLLRALQSGEIRRVGGTTVQKVNVRIISATNQNLAQLVREKRFREDLYYRLRVIHLVLPPLRERRADLPLFLEHAMRRVCERLGRPPARFSPAALRALMAYPFPGNIRELENVVEQAVVLAEGDVIDVGDLPPDVVGAETAAAGPADRSGDGWLGLNDPGTFPTLEEVERRVLEAGLRAFDSKAELARHLGLSRATLYRKLARYGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD1</accession>
    <name>Q67SD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycerol-3-phosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH427</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH427"/>
    <dbReference type="EnsemblBacteria" id="BAD39412">
        <property type="protein sequence ID" value="BAD39412"/>
        <property type="gene ID" value="STH427"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH427"/>
    <dbReference type="eggNOG" id="COG0578">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_5_1_9"/>
    <dbReference type="OMA" id="CIVNAAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="PTHR11985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="7">
        <location>
            <begin position="18"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="DAO_C" evidence="8">
        <location>
            <begin position="401"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="543" mass="59154" checksum="D23FD7CCBD489C71" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG5</accession>
    <name>Q67SG5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH393"/>
    <dbReference type="EnsemblBacteria" id="BAD39378">
        <property type="protein sequence ID" value="BAD39378"/>
        <property type="gene ID" value="STH393"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH393"/>
    <dbReference type="HOGENOM" id="CLU_1864130_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="137" mass="14490" checksum="BB15C4FE1D4D8843" modified="2004-10-11" version="1">MVEFALLAPILLYLVLCIPVFGMFTHSWMVVSGAARAGARAASLLRVQGSREAVARQAADQNMYLRRSDGDLVLFDPARDVQVRVQGGTVTVQVTYHQPSYLPLLSALLGGSGEAGGDTVPITAAATFVIEQGGSLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI8</accession>
    <name>NTPPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dTTP/UTP pyrophosphatase</fullName>
            <shortName evidence="1">dTTPase/UTPase</shortName>
            <ecNumber evidence="1">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="1">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">STH370</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTTP + H2O = diphosphate + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28534"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + UTP = diphosphate + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family. YhdE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194504.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SI8"/>
    <dbReference type="SMR" id="Q67SI8"/>
    <dbReference type="STRING" id="292459.STH370"/>
    <dbReference type="EnsemblBacteria" id="BAD39355">
        <property type="protein sequence ID" value="BAD39355"/>
        <property type="gene ID" value="STH370"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH370"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_2_1_9"/>
    <dbReference type="OMA" id="RIDGDFY"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036221">
        <property type="term" value="F:UTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267443" description="dTTP/UTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="194" mass="20965" checksum="DB7C1AB905E9BEAB" modified="2004-10-11" version="1">MQQLILASSSPRRQELLRQVGIPFVVAVPEVDEHAVHADSPAELVERLALRKARAVSVRYPGAIVLGADTIVVVDGEVLGKPADRAEAEWMLGRLSGRSHQVLTGVALVRGDEELVAHEETVVRFAPLSREQIQWYVETGEPMDKAGAYGIQGRAAALIASISGDYYNVVGLPLHRTVQMLTQFGYPIFTGGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL9</accession>
    <name>Q67SL9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease R</fullName>
            <shortName evidence="6">RNase R</shortName>
            <ecNumber evidence="6">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnr</name>
        <name evidence="9" type="ordered locus">STH339</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194473.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH339"/>
    <dbReference type="EnsemblBacteria" id="BAD39324">
        <property type="protein sequence ID" value="BAD39324"/>
        <property type="gene ID" value="STH339"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH339"/>
    <dbReference type="eggNOG" id="COG0557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_7_0_9"/>
    <dbReference type="OMA" id="DWYEYRS"/>
    <dbReference type="OrthoDB" id="1602988at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040476">
        <property type="entry name" value="CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013223">
        <property type="entry name" value="RNase_B_OB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17876">
        <property type="entry name" value="CSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08206">
        <property type="entry name" value="OB_RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="626"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="719"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="796"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="834" mass="93356" checksum="2BA5AEE2246E98E1" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM9</accession>
    <name>Q67SM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sporulation specific N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194463.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH329"/>
    <dbReference type="EnsemblBacteria" id="BAD39314">
        <property type="protein sequence ID" value="BAD39314"/>
        <property type="gene ID" value="STH329"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH329"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979787_0_0_9"/>
    <dbReference type="OMA" id="GYHNTGA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MurNAc-LAA" evidence="1">
        <location>
            <begin position="81"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="30644" checksum="C8F9703C6AD282A7" modified="2004-10-11" version="1">MVTRIVLDPGHGGWDPGAVANGITEKDYQLAIGLRLRDALLARYDDVEVRMTRETDASVDPAGMSLPPGTARLARELQARVNIANGWPDSVLISLHNDAAGDSRARGGTIYVYGPQSWVAAVAPDGKINHRAPRSYQLAQAMEPIFREMLAKHGIPCNGTKAGDFQVLRNTAGRAVLVEAFFSTSPLDAAAAKTEAFKADLTDAYCRMIAAALGLREKAPATTNPRPVRVVLPSGKVITGELRDSHTWIEVGGVWCPLRAWAELLGFEVLWDNNGPTATVRLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR1</accession>
    <name>Q67SR1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH297</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194432.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH297"/>
    <dbReference type="EnsemblBacteria" id="BAD39282">
        <property type="protein sequence ID" value="BAD39282"/>
        <property type="gene ID" value="STH297"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH297"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OMA" id="ICMEMEP"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="334" mass="37614" checksum="55BCBC9FD23B8B3B" modified="2004-10-11" version="1">MTQPILEVKNLTVEFTVHGRTIRACDNVSLTINRGEIVGIIGESGSGKSTLVFGILNLIQAPGKITSGEVIYYPKDGNPVDLRKLTPQDFARYRWTYLTTVFQAAQNVLNPTLRVKEHFLETAWAHNPNMPEEEILAKARDLLSHVRLEERVLDCYPHQLSGGMKQRTIIALSLILDPDIIFLDEPTTALDVITQWYILEILRRIQKEYGLTMVFCTHDVSIIGSIVDRLGVMYAGQLVEFGPVLTMFKQPTHPYTAGLMSAIPSLRDDVTKRKAIPGHPPDLANLPDSCRFAPRCFACAKGICDGDRKKTEQLYPSHPGQYTRCYSWEKVFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU1</accession>
    <name>Q67SU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39252">
        <property type="protein sequence ID" value="BAD39252"/>
        <property type="gene ID" value="STH267"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH267"/>
    <dbReference type="HOGENOM" id="CLU_2358631_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="10198" checksum="C234C9D86A6E07E3" modified="2004-10-11" version="1">MYTRSQDHCRAISTWIATGISTLISTGVSTPFSTDVSTSPSTSGFHAGERGPMPTSQAWCGPAGSEPGELGLRIHRLGYRRSPLAGLRNSPFALPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T65</accession>
    <name>Q67T65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194278.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH143"/>
    <dbReference type="EnsemblBacteria" id="BAD39128">
        <property type="protein sequence ID" value="BAD39128"/>
        <property type="gene ID" value="STH143"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH143"/>
    <dbReference type="eggNOG" id="ENOG5032W03">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103782_0_0_9"/>
    <dbReference type="OMA" id="FCVPAPC"/>
    <dbReference type="OrthoDB" id="1736943at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="25011" checksum="213ABE34B6041528" modified="2004-10-11" version="1">MARDTDATAGTETIVVPGPIRGGHCPPPREIVLVDARKVFDFCTQEDLLERCFTIPNLGTGATILSCRITQIQCEEVADRQPVNNGGDGRAIVSIQITLTLQIQVLPAMGMQPVTVERTISFPKQVVLCAPEGTDVTCDVEGSCICTVQPNAAQGEPNVCCTIQLCTVLTVTADVKLLVPTFGSVLPRRCPSAASPAGCPPEVEPCEPPVRLAVRRRERDRDRDRDKDCGCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF5</accession>
    <name>Q67TF5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH53</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH53"/>
    <dbReference type="EnsemblBacteria" id="BAD39038">
        <property type="protein sequence ID" value="BAD39038"/>
        <property type="gene ID" value="STH53"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH53"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_8_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="15653" checksum="81B6CC10A95F0919" modified="2004-10-11" version="1">MGKTFLACALGNAACRHGYTVRYYRVPRLLAELATARVDGSLRRILNTLSRTDVLILDDWNLAPISVAESRDLLEVIDDRTQARSTVIASQLPIETWHSVIGDATVADAILDRLVHGAHKLNLKGESMRKIANLQTSELTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J37</accession>
    <name>Q67J37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ParBc, ParB-like nuclease domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197443.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3332"/>
    <dbReference type="EnsemblBacteria" id="BAD42313">
        <property type="protein sequence ID" value="BAD42313"/>
        <property type="gene ID" value="STH3332"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3332"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023853_0_0_9"/>
    <dbReference type="OMA" id="IALSYQR"/>
    <dbReference type="OrthoDB" id="653368at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="28"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="33406" checksum="FBD0A04389EE3ECA" modified="2004-10-11" version="1">MSKKGLGRGIGALIPGIDPADRERLGVVELEIGVIRPNPDQPRKEFDEVRLEELALSIKEHGIVQPILVRKAGDGYEIVAGERRWRAAQLAGLTKVPALVREFSDAERMEIALIENLQREDLNPMEEAEAYRTLMESFGLTQEALAQRLGRSRSQVANTLRLLQLPAQVQDEVRAGRLSMGHAKVLCGVEDPARVVALAEMVIAKGLSVRELEDELTPRERRQRPRARAKGKLPPDLAAVEQQLREQLGTPVKLHWSGDRGRVEITFFDEDGLNRLLEALGVGQRMERPRPPREPFRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J50</accession>
    <name>RS18_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsR</name>
        <name type="ordered locus">STH3319</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD42300" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42300.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197430.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J50"/>
    <dbReference type="SMR" id="Q67J50"/>
    <dbReference type="STRING" id="292459.STH3319"/>
    <dbReference type="EnsemblBacteria" id="BAD42300">
        <property type="protein sequence ID" value="BAD42300"/>
        <property type="gene ID" value="STH3319"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3319"/>
    <dbReference type="eggNOG" id="COG0238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148710_2_2_9"/>
    <dbReference type="OMA" id="QKKYCRF"/>
    <dbReference type="OrthoDB" id="1940575at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000345553" description="30S ribosomal protein S18">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="76" mass="9094" checksum="0D39C8942BCFBF28" modified="2008-07-22" version="2">MRRERGRRARRRVCSFCVDKIEHVDYKDAARLKRYITERGKILPRRISGNCARHQRQLTVAIKRARIMALLPFTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J51</accession>
    <name>Q67J51_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197429.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3318"/>
    <dbReference type="EnsemblBacteria" id="BAD42299">
        <property type="protein sequence ID" value="BAD42299"/>
        <property type="gene ID" value="STH3318"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3318"/>
    <dbReference type="eggNOG" id="COG4241">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068641_2_0_9"/>
    <dbReference type="OrthoDB" id="1112251at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018710">
        <property type="entry name" value="DUF2232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09991">
        <property type="entry name" value="DUF2232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="350" mass="38199" checksum="EDC436D65E6CBE2A" modified="2004-10-11" version="1">MESRSTRVQGLVFGGLMAAMTVVFSLVPGLSVLMPIPLVLAYVRYGGRIAVMTATAATLFTMAFTGVVSAILAIPAGILPGLVFGMGFRRKWKPLTIGLAAVLTFFLGFALEYAVTRVVMFDGRDPFVAMLETPAVQNQVEMMAGVMEQTAAMIESAAADGAATTAQQQMAQQYRDMADLLRRDAVGLVWTLLPASLFFAGAFSSWFNYMLCRLILPRFGHPLPPSTPFAEFRLPIWVIWAYALISLAVPLLAGGDMTALPWWGKLLLNVFTPLMFILVLAGLAVAYGWMRRRGLAKGLAVLILAVAFLLLGQLGMQLLVLLAMWDTVFDVRGLGHGLWKRTEETRQREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J99</accession>
    <name>Q67J99_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Conserved membrane-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0702 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197382.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3270"/>
    <dbReference type="EnsemblBacteria" id="BAD42251">
        <property type="protein sequence ID" value="BAD42251"/>
        <property type="gene ID" value="STH3270"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3270"/>
    <dbReference type="eggNOG" id="COG2323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077149_0_2_9"/>
    <dbReference type="OMA" id="IMNGKIM"/>
    <dbReference type="OrthoDB" id="1884218at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.240.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007353">
        <property type="entry name" value="DUF421"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023090">
        <property type="entry name" value="UPF0702_alpha/beta_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04239">
        <property type="entry name" value="DUF421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="26961" checksum="2CD408BB195DF13F" modified="2004-10-11" version="1">MGEVSVVLVRSAITFFNLLILCRLLGKTQVSQLTFFEYVSGITLGSLAAELSTNLSLRPWPVFVGLFAWGGFTLLTQLVALKSRWMAKMLDGEPVILVYRGQILEENLRRLRMRESELAELLRSQSVFHFDEVELAVLEPRGTLSVLRTADTQPVTPADLGIPASSRGLGIELVVDGEVMDQNLRRLGVNRTWLMAKLKEQGIRSPAEAFLAVVDADGKLYVDRYTDRVPKSHDLSDYPGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB7</accession>
    <name>Q67JB7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TatD-related DNase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197364.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3251"/>
    <dbReference type="EnsemblBacteria" id="BAD42233">
        <property type="protein sequence ID" value="BAD42233"/>
        <property type="gene ID" value="STH3251"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3251"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_4_0_9"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="29129" checksum="5FAD027D93EC9D78" modified="2004-10-11" version="1">MLFNTHSHVDTGRQFAVDRDEVVARAREMGVSQLMVIGFERALIPVTLAFAERHDWVWAAVGIHPTSALEWGPDAERELREAARSPKVRAIGEVGLDYYWKDKAPFEVQRDVFRQQIRLARDLGLPLVIHNRDAHEDVVRILEEEGADEVGGIMHCFSGDWEMAERCLALNFYIGIGGTVTYKNNPVGREVAQRLPLDRIVLETDDPYLAPVPYRGKRNEPGYVRIVAEFVAELRGLTLEAIAEATMANARRALRLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE1</accession>
    <name>Q67JE1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197340.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3227"/>
    <dbReference type="EnsemblBacteria" id="BAD42209">
        <property type="protein sequence ID" value="BAD42209"/>
        <property type="gene ID" value="STH3227"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3227"/>
    <dbReference type="HOGENOM" id="CLU_113225_2_1_9"/>
    <dbReference type="OMA" id="RFYIVWG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014242">
        <property type="entry name" value="Spore_cortex_biosyn_YabQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019074">
        <property type="entry name" value="YabQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09578">
        <property type="entry name" value="Spore_YabQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02893">
        <property type="entry name" value="spore_yabQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="175" mass="20255" checksum="D90DB4593DD7FE90" modified="2004-10-11" version="1">MLRMELQFYALFMTVLSGIGVGLLFDLLRAVRRFLRPGPLLAALGDLLFWGAATAMVGTGLFLGNWGEYRFYVLVGLLTGLVLHFALASPAVLWLADRLLHAVAWVLKVLWELAMRLVWFPLQAVAAVLWRLGRGLGTWLGRRLRGPYRWLRLRYLLARRRLRRAWRHWFRGPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR4</accession>
    <name>Q67JR4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3104"/>
    <dbReference type="EnsemblBacteria" id="BAD42086">
        <property type="protein sequence ID" value="BAD42086"/>
        <property type="gene ID" value="STH3104"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3104"/>
    <dbReference type="HOGENOM" id="CLU_3158621_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="48" mass="5721" checksum="68C5FC4B7856A5AB" modified="2004-10-11" version="1">MLRQTHRSYYAAFRSSHWLARLNLIYLAVVPAFLVGSLIYLVWFYLTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU2</accession>
    <name>RS10_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsJ</name>
        <name type="ordered locus">STH3076</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197191.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU2"/>
    <dbReference type="SMR" id="Q67JU2"/>
    <dbReference type="STRING" id="292459.STH3076"/>
    <dbReference type="EnsemblBacteria" id="BAD42058">
        <property type="protein sequence ID" value="BAD42058"/>
        <property type="gene ID" value="STH3076"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3076"/>
    <dbReference type="eggNOG" id="COG0051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_1_3_9"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="2012682at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000237104" description="30S ribosomal protein S10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <sequence length="102" mass="11349" checksum="8532BE1D8923CFA1" modified="2004-10-11" version="1">MAKQKIRIRLRAFDHKILDSSAAKIVETAIRTGATVSGPIPLPTEKSIYTILTAPNGEKDIREQFEMRTHKRLIDIVEPGSKTVEALMKLDLPAGVDIEIKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU9</accession>
    <name>RS3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsC</name>
        <name type="ordered locus">STH3069</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197184.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU9"/>
    <dbReference type="SMR" id="Q67JU9"/>
    <dbReference type="STRING" id="292459.STH3069"/>
    <dbReference type="PRIDE" id="Q67JU9"/>
    <dbReference type="EnsemblBacteria" id="BAD42051">
        <property type="protein sequence ID" value="BAD42051"/>
        <property type="gene ID" value="STH3069"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3069"/>
    <dbReference type="eggNOG" id="COG0092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_0_2_9"/>
    <dbReference type="OMA" id="KTNPIGN"/>
    <dbReference type="OrthoDB" id="1132353at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130216" description="30S ribosomal protein S3">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="39"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="267" mass="29663" checksum="2D4B6597D451EAE1" modified="2004-10-11" version="1">MGQKVHPKGLRIGIVKDWDTRWYADKRNFSDLLVEDVKIRKYIKKTLYAAGISRIEIERAGGRIRIIVHTAKPGMVIGRQGQGIAELQNQLAQMTGKQISLDVKEIRLPELDAQLVAESIAQQLEKRMSHRRAMKQAIQRAMRLGAQGVRIIVSGRIGGAEIARSERAWEGTVPLHTLRADIDYGFAEADTTFGKIGVKVWIYAGEAAQKAAAGDRRERGERRERGGRRGGRGRGQGGQRMSREERAALERAAIEAGPKPVNREGGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV9</accession>
    <name>RL18_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplR</name>
        <name type="ordered locus">STH3059</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197174.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV9"/>
    <dbReference type="SMR" id="Q67JV9"/>
    <dbReference type="STRING" id="292459.STH3059"/>
    <dbReference type="EnsemblBacteria" id="BAD42041">
        <property type="protein sequence ID" value="BAD42041"/>
        <property type="gene ID" value="STH3059"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3059"/>
    <dbReference type="eggNOG" id="COG0256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098841_0_1_9"/>
    <dbReference type="OMA" id="NKQIYAQ"/>
    <dbReference type="OrthoDB" id="1963491at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131365" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="123" mass="13482" checksum="FC6589DA80914D20" modified="2004-10-11" version="1">MIKKLDRNKARRKRQLRVRKTVHGTPERPRLNVFRSNQNIYAQIIDDTVGRTLVSASTLDPEVRSRLQGSGGNKAAAAIVGEVVAKRALAAGVTKVVFDRAGYLYHGRVAALAEAAREAGLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW3</accession>
    <name>Q67JW3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="11" type="ordered locus">STH3055</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197170.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3055"/>
    <dbReference type="EnsemblBacteria" id="BAD42037">
        <property type="protein sequence ID" value="BAD42037"/>
        <property type="gene ID" value="STH3055"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3055"/>
    <dbReference type="eggNOG" id="COG0201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030313_0_2_9"/>
    <dbReference type="OMA" id="FAMWLGE"/>
    <dbReference type="OrthoDB" id="1567535at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="422" mass="46807" checksum="2424577CA3D74D04" modified="2004-10-11" version="1">MLETLRNSMRVPGLRKRLLFTIGMLVVIRICAHIPVPGVNLDRLTEVFGAGGLFDFFDIFAGGAFSQFAIVAMGVTPYINSSIIMQLLTVVIPRLEELQKEGEEGRKKIQQYTRYGAVVLGLVQAFGIGYALRSYGAFYSNSWTTLFVIMVSLTAGTALVMWIGEEITEKGIGNGISLIIFANIVSRLPYSIYNEILLLRAGQRNIFQPLLVAVVALLMVVFVVIINEAVRKIPVEYAKRVVGRRVVGGQRTHLPIRINQAGVIPLIFASSLMYFPITIAGFFPNSDFAVFVGRYLDTRSWWFMLINALLIVFFTYFYTAITFNPENVADNIKKNGGFIPGYRPGRPTAEYLERVSSRLTLVGGLFLAAVTILPITIMSLAGVQNAYIGGSSLLIVVSVALETTKQLEAQLVMRHYQGFMRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K08</accession>
    <name>Q67K08_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fliM</name>
        <name evidence="11" type="ordered locus">STH3009</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliM family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197124.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3009"/>
    <dbReference type="EnsemblBacteria" id="BAD41992">
        <property type="protein sequence ID" value="BAD41992"/>
        <property type="gene ID" value="STH3009"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3009"/>
    <dbReference type="eggNOG" id="COG1868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052646_0_0_9"/>
    <dbReference type="OMA" id="NPQFTQI"/>
    <dbReference type="OrthoDB" id="1748746at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17908">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001689">
        <property type="entry name" value="Flag_FliM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02154">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002888">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00955">
        <property type="entry name" value="FLGMOTORFLIM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01397">
        <property type="entry name" value="fliM_switch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="10">
        <location>
            <begin position="237"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="34335" checksum="CEA072DA0B3B54A4" modified="2004-10-11" version="1">MENPLSQAEIDALVMSTGTNIRTYDFRRPSKFNKDLIRTLVLVHENLARLLQSYLSAGLRTWVQITVRSTNQYTFSEFTQLLPNPTIALSFRLNPLPGMCLLEISHNIAFAIVERVFGGPGSDLQPQRELTEIELGIIQRVVRDTFGPMKEAWRNVAEIDPAIEAVETNPVFIQIENPTEVVAAITLAVEIGEHLGHMTFAFPYSTVEPVLSRLSPQSWLAETKHKSPSDEESLRRAVQEAPLPVKALLGKTRMRLGEFLALQVGDVIRLDARVGGELPVFVGNKLAFQAKPGVVGERMAVQITRRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K67</accession>
    <name>Q67K67_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ychF</name>
        <name evidence="7" type="ordered locus">STH2948</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197064.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q67K67"/>
    <dbReference type="STRING" id="292459.STH2948"/>
    <dbReference type="EnsemblBacteria" id="BAD41931">
        <property type="protein sequence ID" value="BAD41931"/>
        <property type="gene ID" value="STH2948"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2948"/>
    <dbReference type="eggNOG" id="COG0012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_0_1_9"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="3"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="282"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="39748" checksum="F97F6C8D056B45FD" modified="2004-10-11" version="1">MGLSIGIVGLPNVGKSTLFNAITRAGAEAANYPFCTIEPNVGVVDVPDARLPVLAKMFNSARIVPTSIKFIDIAGIVKGASKGEGLGNKFLHHIREVDAIAHVVRCFEDPNVTHVSGRVDPLSDIEVINLELILADLETIERRYERSAKAAKAGVKEAQVEHGLLSRLKEALEAGLPARSVAIEGDEEARVLRDLHLITAKPVMYIANVAEEHVANPFAVPYVAQVKELADREGSEVVPISARIESELAEMDDEERAAFLADLGLAESGLDRVIRHGYHLLNLISFLTAGETEARAWTIRRGTKAPQAAGVIHSDMERGFIRAEVVSYDDLVTAGSMAAAREAGKVRLEGKDYVMQDGDVVTFRFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC0</accession>
    <name>Q67KC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197012.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2895"/>
    <dbReference type="EnsemblBacteria" id="BAD41878">
        <property type="protein sequence ID" value="BAD41878"/>
        <property type="gene ID" value="STH2895"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2895"/>
    <dbReference type="HOGENOM" id="CLU_709651_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="389" mass="42618" checksum="94F7033F0F98273A" modified="2004-10-11" version="1">MTNIVHPRPTDFAHEPLVHWTADGLFAAPWTGRPDTEEPADVEELSARLNQEPAHREDITLALGHLLAVRAHCRRAASALDHLTCRRHYADALKECESALFRLHSCEGADAAALTAWALGVHALIRAQTTLTQDPTEARALAAEALGRLTGPQTALALGAGADALSALKLQQETVELEDRLLRLPAEVRVMTEAAEQAAESRLNALNLAVRALRRRYAQAFAWAVGWGVLNLLLMAAAPINALLLPAVPWSIPCIVALPILWWATWAVPFRDGLPFFGFVRRLRLEAIADLRAAAGDLEPPGDRLAAEIGPLLEEGAQAERRLRQFYLFRLPEQYETVWKARAIAEGALARLKGDWVADMIDAPAAPLREVMEETERIAALIGVRLAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF2</accession>
    <name>Q67KF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2861</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196980.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2861"/>
    <dbReference type="EnsemblBacteria" id="BAD41846">
        <property type="protein sequence ID" value="BAD41846"/>
        <property type="gene ID" value="STH2861"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2861"/>
    <dbReference type="eggNOG" id="COG2252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024508_0_1_9"/>
    <dbReference type="OMA" id="IDMFDSI"/>
    <dbReference type="OrthoDB" id="558559at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="445" mass="46389" checksum="A30620AFB40C5F2E" modified="2004-10-11" version="1">MAAREAIRPSQTSEGLLDRVFKLTENGTDVKREILAGLTTFVTMSYIIAVNPDILSIGTGMEKSAIFMATILAAALSTLAMGLVANWPVALAPGMGLNAFFAFTLCGSLGLPWQQALAAVFCSGVLALIVTLTGLRELMIKAIPLSLRHAVSAGIGFFIMLIGLINAGIVEASEATTIALGDFSRPGTMLALIGLIITGVLVARRVTGGILLGILITTLIGIPMGVTQLPSSLVSAPPSLAPTFLQLDFDVLGNPAMFAVIFAMFFTDLFDTIGTFVGVSSKAGLMDEKGNMKGGNRALVMDSLGTIIGSLLGTSNTTTYVESAAGVSAGGRTGLTAVTVAVLFLLSSFFSDIFLAIPGAATAPALIIVGIMMASSITQIDLDDFAIALPAIITALMMPFAYSIAEGLALGFIAYAVIYLLTGRAKEVHWMMWVLAGFFILHFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG4</accession>
    <name>Q67KG4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2 7">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purD</name>
        <name evidence="10" type="ordered locus">STH2849</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196968.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2849"/>
    <dbReference type="EnsemblBacteria" id="BAD41834">
        <property type="protein sequence ID" value="BAD41834"/>
        <property type="gene ID" value="STH2849"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2849"/>
    <dbReference type="eggNOG" id="COG0151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_1_9"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="932854at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="108"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="422" mass="44014" checksum="2B342B819FBD84CD" modified="2004-10-11" version="1">MKVLIVGGGGREHALAWKAIQSRHIDELHAAPGNPGIGRFAWCHPDVEATDVAGQVDLARRLGIDLVIVGPEAPLAAGLVDALTAAGIPAFGPTAAAARIEASKAYAKEVMTAAGVPTAAYADFTDYQQALAYIEARPGPVVIKADGLAAGKGVQVCADLDEARAALRAAMVEGAFDQAGSRVVIEEVLEGQEVSVLAFSDGRTVKQMVAAQDHKRAGEGDTGPNTGGMGAYAPVPAYTADMAEAVQRRILEPTIAELARRGTPFVGCLFAGLMLTADGPKVIEFNARFGDPETQAVLPLLENDLLEVIQACLEARLHEVDLRFRPGAAVNVVLASAGYPGKYVKGLPISGLVEADQVGVTVFHAGTAFNEDGQVVTAGGRVLGVMATGPTLRDALAMAYAGVDQIQFEGKTYRRDIGWRAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH8</accession>
    <name>HIS5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisH</fullName>
            <shortName evidence="1">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisH</name>
        <name type="ordered locus">STH2835</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196954.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KH8"/>
    <dbReference type="SMR" id="Q67KH8"/>
    <dbReference type="STRING" id="292459.STH2835"/>
    <dbReference type="EnsemblBacteria" id="BAD41820">
        <property type="protein sequence ID" value="BAD41820"/>
        <property type="gene ID" value="STH2835"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2835"/>
    <dbReference type="eggNOG" id="COG0118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_2_9"/>
    <dbReference type="OMA" id="WVYFVHS"/>
    <dbReference type="OrthoDB" id="1726024at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000231763" description="Imidazole glycerol phosphate synthase subunit HisH">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <sequence length="212" mass="22294" checksum="AD12D92829F95718" modified="2004-10-11" version="1">MARIVIVDYGMGNLASVRNALRAVGFEAAVSDDPAAVAGADGLVLPGVGAFGTGMQNLARRGLDQAVRQAAAAGRPVLGICLGMQLLLAEGDEGGPRPGLGLLEGRVARLPDGLPLPQIGWNLVEPQRDHPLFAGLPTPFWAYFDHAYAVEGEPPSTALALTDYGRTYPSVVGRGNLLGIQFHPEKSSRAGLRMLANWGRMVCDLISTRPST</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI0</accession>
    <name>HIS6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisF</fullName>
            <shortName evidence="1">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisF</name>
        <name type="ordered locus">STH2833</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196952.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KI0"/>
    <dbReference type="SMR" id="Q67KI0"/>
    <dbReference type="STRING" id="292459.STH2833"/>
    <dbReference type="EnsemblBacteria" id="BAD41818">
        <property type="protein sequence ID" value="BAD41818"/>
        <property type="gene ID" value="STH2833"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2833"/>
    <dbReference type="eggNOG" id="COG0107">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_9"/>
    <dbReference type="OMA" id="IFHYKET"/>
    <dbReference type="OrthoDB" id="1522718at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142244" description="Imidazole glycerol phosphate synthase subunit HisF">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <sequence length="254" mass="26871" checksum="A93A1335E786C3C8" modified="2004-10-11" version="1">MPLAKRIIPCLDIRDGRVVKNVQFHLNTWDAGDPVALAAEYDRQGADEIVFLDINASWEGRSATLDVLSRAAEQVFVPLTIGGGVSAVEHVKAYLRAGADKVSVNTAAVQRPDLIDEIADLFGSSTLVVAIDCKRRPEGGWEVYLHGGRTPTGIDAVAWAEEAARRGAGELLVTSMDADGTRQGYDIALHQALADAVGVPVIASGGAGSPEDILEVLTVGRADAALAASIFHSGRHTVGEVKAFLRERGVLVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP9</accession>
    <name>Q67KP9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2763"/>
    <dbReference type="EnsemblBacteria" id="BAD41748">
        <property type="protein sequence ID" value="BAD41748"/>
        <property type="gene ID" value="STH2763"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2763"/>
    <dbReference type="HOGENOM" id="CLU_1659842_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="18318" checksum="C140B35B6CCE833E" modified="2004-10-11" version="1">MGSGRGSGQVLQSPVLWGRGLRLGRTRIDGKLMERSGLDEVNLRRARRAFQQRWLRTPPPERRRLAEAGTVARFRYVIDTLRAAWSPEVWSLWLRYLEHTDEEIDEMMAGEYPISPYMVRVSSALLGVTVDFLEAGCWPAQDYDGHDIDVCPQWQFTHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ4</accession>
    <name>Q67KQ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2758"/>
    <dbReference type="EnsemblBacteria" id="BAD41743">
        <property type="protein sequence ID" value="BAD41743"/>
        <property type="gene ID" value="STH2758"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2758"/>
    <dbReference type="HOGENOM" id="CLU_894081_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="34416" checksum="4900F59D9A39AA2D" modified="2004-10-11" version="1">MDKGAGPSDGSQATAPERDWEEQARRADERLAELRRELSHPVFVPTQVPEGLHPQMPSQSDRLVSIAYVDDDLNRVLRVSSGPLGCCIDADPNKMVAGGTPIRDGREARFIPYQPEFGGNILWWQEDGAYVALSGPHLTEEDLFAIAEFLSPTATLTGPPDALPPDVYPPDYRTEVPQVDAVIDAFLAKDVHRLASCDMLRCEEGAEGALEPVVTWGSCEVNNTWTGWDTVRSLVEGWVGEPRLLYAAYRVQGIGDWAYLVQFAKYRHDQRPKSAVLDGEGRILQLWMGCGALFDMQADHYEPIAPPRTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU3</accession>
    <name>Q67KU3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2718"/>
    <dbReference type="EnsemblBacteria" id="BAD41703">
        <property type="protein sequence ID" value="BAD41703"/>
        <property type="gene ID" value="STH2718"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2718"/>
    <dbReference type="HOGENOM" id="CLU_2345622_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="97" mass="10670" checksum="C1AE5087266DD219" modified="2004-10-11" version="1">MERLVPSSWSSPAPEIFVHSSPGAARHSRSAGGRHGFTAAQANGSHPELYKLHCSKLSRGGRLAGRPRPWRQTNASVDERHCRQSSHCQIARATTMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU8</accession>
    <name>Q67KU8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 4">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2713</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2713"/>
    <dbReference type="EnsemblBacteria" id="BAD41698">
        <property type="protein sequence ID" value="BAD41698"/>
        <property type="gene ID" value="STH2713"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2713"/>
    <dbReference type="eggNOG" id="COG1210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_9"/>
    <dbReference type="OMA" id="KRYDVGN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="5">
        <location>
            <begin position="6"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="32465" checksum="A9E33F4224CEEB3E" modified="2004-10-11" version="1">MMRVRKAVIPAAGLGTRFLPATKAQPKEMLPLVDKPIIQYVVEEAVASGIEDIIIVTSRGKRAIEDHFDRNLELELMLEGRNGELLAAVRRLSDMADIHFIRQKQPMGLGHAVYQARRHVGNEPFAVLLGDEVFIGATPCLKQLIDTYEETGGSVIAVRPVPLEEVSRYGVIAPEPAGERLHRAVDLVEKPSVTKAPSNLAIVGRYVLDPAIFEILSDLPPGRNGEIQLTDGLRELNRFSQVYAYEPAVKRYDVGEKLGFIQATIELALERNELADGLRVYLEDLVAGRPTANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV9</accession>
    <name>Q67KV9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2702</name>
        <name evidence="2" type="ordered locus">STH273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">IAM14863</strain>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194408.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2702"/>
    <dbReference type="EnsemblBacteria" id="BAD39258">
        <property type="protein sequence ID" value="BAD39258"/>
        <property type="gene ID" value="STH273"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41687">
        <property type="protein sequence ID" value="BAD41687"/>
        <property type="gene ID" value="STH2702"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2702"/>
    <dbReference type="KEGG" id="sth:STH273"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_7_0_9"/>
    <dbReference type="OMA" id="DINWRAS"/>
    <dbReference type="OrthoDB" id="1792718at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="98"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="28450" checksum="807EE3964D275B50" modified="2004-10-11" version="1">MPLQTTIERLREMRLRGMADALVRQQQQPELQSLSFEERLELLVDQEWLDRQNRRLARLLKEAKFRVAACMEDVDYQHPRGLDRTVMRSLAACQWVEYGQHILIVGPTGVGKTFIACALGNAACRHGYTVRYYRVPRLLAELATARVDGSLRRILNTLARTDVLILDDWGLAPISVAESRDLLEVIDDRTQARSTVIASQLPIETWHSVIGDATVADAILDRLVHGAHKLNLKRESMRKIANLQTSQSSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW4</accession>
    <name>Q67KW4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2697"/>
    <dbReference type="EnsemblBacteria" id="BAD41682">
        <property type="protein sequence ID" value="BAD41682"/>
        <property type="gene ID" value="STH2697"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2697"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_17_0_9"/>
    <dbReference type="OMA" id="PDFEEGW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="141"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="361" mass="39186" checksum="C2668896B37D86DF" modified="2004-10-11" version="1">MKPYRIGIVGYSFGRIHAEAYRQIPFYLDVGAPIELVGVATSRPETARQAQAEAGFALATADWRELVASPDVDIIHVCTPDDLHHPIAAAALAAGKHVYCEKPLARTVAEAEDLYDRARASGRVAQIAFQMRFGSAVQAARRLIRDGTLGEITSFRLTFLHSGYEDPERPLTWRLDSARTGGGVLYDLGSHAVDLLTFLLGRPRRVMGLTRTFIKERPVATGSDRKAPVKVDDVALVTMEMEAGCVGVMEATRLATGVRRGPDLTIYGTRGSVAFNGFTNHNELNLYLKQEKVPGLSGWETINTGGRNPVPLSVAAQAAFLTAVRDGRTDAEPNFATGLQVQRVLAAVQESARTGQWVSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW8</accession>
    <name>Q67KW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2693"/>
    <dbReference type="EnsemblBacteria" id="BAD41678">
        <property type="protein sequence ID" value="BAD41678"/>
        <property type="gene ID" value="STH2693"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2693"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_8_9"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="166" mass="18760" checksum="12919F80F61565D5" modified="2004-10-11" version="1">MKQQADTAPADRFTPENILPLLQTIADLVEKRCHRFLSRNYGLTMPQYQLLLAAMNGGATTLGSLADELNCSRGNLTGVADRLERDGWLVRERSTEDRRVVNIRLTEKGKKVWEIRNALAKEMAEIAKIWSPDEMTIMLRSLERLYTELKGEAVKPTGEKPATEAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY3</accession>
    <name>Q67KY3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196800.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41663">
        <property type="protein sequence ID" value="BAD41663"/>
        <property type="gene ID" value="STH2678"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2678"/>
    <dbReference type="HOGENOM" id="CLU_1694629_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="155" mass="16182" checksum="9AD0E8A9BAB335D7" modified="2004-10-11" version="1">MIWFLLFLAVLGIAWFLFVPRTGDETALPLDGDRQDTDLPNVSAHATSNVEPTLTHPAALFTADGTDMPTAPAEAGQDGGGQFTAGQDAGERAAAVQGAPEPTYAGQDAAHSAAHAAQEAAPDAVQNAARKTAELGWMPDNYQEPRLDAPPGNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L08</accession>
    <name>Q67L08_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2653</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2653"/>
    <dbReference type="EnsemblBacteria" id="BAD41638">
        <property type="protein sequence ID" value="BAD41638"/>
        <property type="gene ID" value="STH2653"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2653"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_2_9"/>
    <dbReference type="OMA" id="WFPIGLS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="97"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="33822" checksum="5DC643D887D0D496" modified="2004-10-11" version="1">MRYAARRLLLMLPVLFGVTLLVFLLFYLTPGDPVRAILGQEAQGLSVEDIERLRHQLGLDRPWYEQYLSFLGKAVQGDLGRTFRGDRPVAGEIAARFPTTLRLTVISLTVAALVGVPLGVVGAVRRNRWADHLVMLIALLGVSMPTFWVGIMLMQLFALKLRILPPSGTGTWRHMVLPAATVALASIAFIARMTRSSLIDALREDYVRTARAKGVAERRVVFRHALRNAFIPVLTTLGLEFGSLLGGAVVVESVFSLPGIGRLTVDAIKGRDLPLIQGTILFVAVVFSLVNLIVDLGYAGLDPRIRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L20</accession>
    <name>Q67L20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fumarate hydratase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2641</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196763.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2641"/>
    <dbReference type="EnsemblBacteria" id="BAD41626">
        <property type="protein sequence ID" value="BAD41626"/>
        <property type="gene ID" value="STH2641"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2641"/>
    <dbReference type="eggNOG" id="COG1951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_9"/>
    <dbReference type="OMA" id="IETYPTH"/>
    <dbReference type="OrthoDB" id="972093at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="29985" checksum="95AF0CA3FB5633A2" modified="2004-10-11" version="1">MRELHVDQVAEAVSNLVMEANYVLEPDVAAALEKARCDEQSAAGRAVLEQLVENADLARNERVPMCQDTGNALVFLEVGQDLHVVGGDLYEAVNRGVRKGYTDGYLRKSIVDDPLRRKNTGDNTPAFIYTDIVPGDRLRIRVATKGGGAENMGQLKMLPPSAGWEGAKRFIVEAVAAAGPNACPPVLVGVGIGGNFDKVALLAKKALLRPVGEPNPDPAWAAREQELLAEINKLGIGPMGLGGRVTAFAVHIETMPCHITALPVAVNIDCHAHRHKEVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L90</accession>
    <name>Q67L90_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative enoyl-(Acyl-carrier-protein) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196693.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2571"/>
    <dbReference type="EnsemblBacteria" id="BAD41556">
        <property type="protein sequence ID" value="BAD41556"/>
        <property type="gene ID" value="STH2571"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2571"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_1_0_9"/>
    <dbReference type="OMA" id="IDDLPSC"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="5"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="320" mass="33799" checksum="3D8CCA30AA0071CE" modified="2004-10-11" version="1">MLCTRLCELLSIEYPIIQGGLAYLARAELCAAVSEAGGLGQITAATLGSPEELREEIRKVRALTRKPFGVNFAMGYRSLEPYIDAALEEGVRIVTITGGNPEPYMKYIDARVPGVMKIILVAGVRAAQKAEALGADAVIAVGYDGGGHLGRDDVGTMALIPRIVDSVKIPVAASGGIVDGRGLMAALALGADGIEMGTRFVAVQECPAHEAYKQLLLETRENETVIMERSIGRPARVLRTGGALRVLEAEARGATLEELLPLISGKVNARAALEGDMEEGFVYAGQGVGLIHDIPTVAELFARILAEARAIGERLRGLGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA3</accession>
    <name>SYT_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="1">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrS</name>
        <name type="ordered locus">STH2558</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196681.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LA3"/>
    <dbReference type="SMR" id="Q67LA3"/>
    <dbReference type="STRING" id="292459.STH2558"/>
    <dbReference type="EnsemblBacteria" id="BAD41543">
        <property type="protein sequence ID" value="BAD41543"/>
        <property type="gene ID" value="STH2558"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2558"/>
    <dbReference type="eggNOG" id="COG0441">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_9"/>
    <dbReference type="OMA" id="DAHIFML"/>
    <dbReference type="OrthoDB" id="900765at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000101068" description="Threonine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="1">
        <location>
            <begin position="245"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01228"/>
        </source>
    </evidence>
    <sequence length="651" mass="74077" checksum="9D240CE16FE6BD98" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD3</accession>
    <name>Q67LD3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2528</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196651.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2528"/>
    <dbReference type="EnsemblBacteria" id="BAD41513">
        <property type="protein sequence ID" value="BAD41513"/>
        <property type="gene ID" value="STH2528"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2528"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_4_9"/>
    <dbReference type="OMA" id="VKSRMRY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="119"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="183" mass="20609" checksum="49A9CE95C2A01056" modified="2004-10-11" version="1">MEAYSDEQRLVLRAQRDPEAFGELYDMHFDRIYAYIHRKTGDRQVAEDLTADTFLKALAHIQHYRYTGQPFAAWLYRIAANVVIDYYRVHRPTVQLDEGLSAAGVGPEPEEAALALDDRQAVSRALQALSPDQQDVVLMRFAGDMKLKDIAAAMGKTEGAVKALLFRALNTLRRKLSESGVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG0</accession>
    <name>Q67LG0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196624.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2501"/>
    <dbReference type="EnsemblBacteria" id="BAD41486">
        <property type="protein sequence ID" value="BAD41486"/>
        <property type="gene ID" value="STH2501"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2501"/>
    <dbReference type="eggNOG" id="COG1410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079580_2_0_9"/>
    <dbReference type="OMA" id="NINWRYS"/>
    <dbReference type="OrthoDB" id="1410233at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017342">
        <property type="entry name" value="S-AdoMet-dep_Met_synth_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037984">
        <property type="entry name" value="Met_synth_TM0269_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="SSF56507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="20278" checksum="41A902BD6A089ABF" modified="2004-10-11" version="1">MERGIALTREAAAPAASAAYCAVTVAEDRVSAAVPGVAWQSRSLARLLAGADAASLVAVTLGAGVDELIRDLFAREEFALATIVDAAGSALVHGLAEWVRGALLQEAEAGAAAGVTLTPLYGPGYGDWKVEEQPGLVALAGGEAIGLRCTPTCYLVPQKSLVGLIGWLPSGGRDWPAVGCTRCTLADCAYRVRTSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK3</accession>
    <name>Q67LK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196581.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2458"/>
    <dbReference type="EnsemblBacteria" id="BAD41443">
        <property type="protein sequence ID" value="BAD41443"/>
        <property type="gene ID" value="STH2458"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2458"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_5_9"/>
    <dbReference type="OMA" id="FTDQINP"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="236" mass="25670" checksum="D29DE4FCC05FD989" modified="2004-10-11" version="1">MPQVIGHRGAAGTHPENTMVSFRRAFELGVDGIEFDVHRTADGHLVVIHDPAVDRTTDGSGLVMAMTLEQIRALDAGSWKDPRFAGERVPTLQELVRATPPGVRLYLELKAGSVHYPGIEQELVDLLKAEGVLDRTQVSSFDHRALVKLKEICPELPLGMLTSSNLVDPVGMAKAIGCEAIHPAWPWVTPEYVEAAHAAGLKVNCWTVNDPLALALMQNAGVDGIMTDYPERLRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN2</accession>
    <name>Q67LN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196552.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2429"/>
    <dbReference type="EnsemblBacteria" id="BAD41414">
        <property type="protein sequence ID" value="BAD41414"/>
        <property type="gene ID" value="STH2429"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2429"/>
    <dbReference type="HOGENOM" id="CLU_2248741_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="11463" checksum="C9064EAC70EFA2F3" modified="2004-10-11" version="1">MAPWTDDLVYRVQALAELADDLHNRLAQVEAGLERQRAESARLWAEHREVVAEMRAALLPLKSRIGFVEGRLDGKKETASLLISSFLSAVAVLVSALLGILNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN5</accession>
    <name>Q67LN5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196549.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2426"/>
    <dbReference type="EnsemblBacteria" id="BAD41411">
        <property type="protein sequence ID" value="BAD41411"/>
        <property type="gene ID" value="STH2426"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2426"/>
    <dbReference type="HOGENOM" id="CLU_2014105_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13050" checksum="E4CBB1F0F8B9B924" modified="2004-10-11" version="1">MVLTVIGLALLGAALLLPNVLALRLAFAGAAEREAARVEARAAARRSGGQAAARVRVRIPAPRLVRRGYLWKALSPLLFALSVMFGIAVGGTTGYVLMGLGLLNLLFGLWGWDEYVPGHTGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ9</accession>
    <name>Q67LQ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">THUMP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2402</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196525.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2402"/>
    <dbReference type="EnsemblBacteria" id="BAD41387">
        <property type="protein sequence ID" value="BAD41387"/>
        <property type="gene ID" value="STH2402"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2402"/>
    <dbReference type="eggNOG" id="COG0116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032119_3_1_9"/>
    <dbReference type="OMA" id="GFMYKAN"/>
    <dbReference type="OrthoDB" id="1772619at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="4">
        <location>
            <begin position="48"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="375"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="415" mass="46086" checksum="4B2764515363C44B" modified="2004-10-11" version="1">MSDTQQLTLIATAAMGLEAVVARELKGLGYDQQIVENGRVRFRGDARAICRANLWLRTADRVLVEMASFPATTFEELFQGVRAVPWEEWIPAEGRFIVNGRSHQSQLSSVPACQKVAEKAVVERLRQRYPLEWFPKSGARYSIEVSLLKDVATVTLDTTGPGLHKRGYRKLVTEAPIKETLAAAMVLLSRWHPDRPLLDPCCGSGTIPIEAALIGHNLAPGLHRSFDAETWGCIPARLWDEAREEGFDLADYDRPLDIRGSDIDPGALELAAHHLRAAGLSRSVRLERKPLQEIGRDAPYGYLITNPPYGERMGEREAVEALYRDIGRLVRRLQDWSAFVITPHKGFERHFGRRADRKRKLYNGRIECDLYQYEGPYHGRPVPGTGGGPASGESQGRPAPGAGRARPAPGGGDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR3</accession>
    <name>Q67LR3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stage 0 sporulation protein A homolog</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH2398</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196521.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2398"/>
    <dbReference type="EnsemblBacteria" id="BAD41383">
        <property type="protein sequence ID" value="BAD41383"/>
        <property type="gene ID" value="STH2398"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2398"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_368778_0_0_9"/>
    <dbReference type="OMA" id="VFGEFNQ"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="12">
        <location>
            <begin position="76"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="143"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="12">
        <location>
            <begin position="218"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="275"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="509"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="635"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="558"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD41383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="755" mass="82974" checksum="7243777939CAEE24" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV9</accession>
    <name>Q67LV9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196475.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2352"/>
    <dbReference type="EnsemblBacteria" id="BAD41337">
        <property type="protein sequence ID" value="BAD41337"/>
        <property type="gene ID" value="STH2352"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2352"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_3_9"/>
    <dbReference type="OMA" id="YETYKHS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="202" mass="21730" checksum="63D93763985F7906" modified="2004-10-11" version="1">MRDLLTALVRRLHGLVDDETLAHSGRVAYLAAALGEALGLSREMTRTLYLGGLLHDIGKGVVPAEVLHKPGRLLPEERRLVEAHAAAGESMVLQLAPEMAPDIVDVVRHHHERLDGSGYPDRLCAGDISPAVRIIAVADVYDALINDRPYRRALSPEEALGVLDEEVRCGRLDADVVAALRRVTADEDGLSRAADGRGGQCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX5</accession>
    <name>Q67LX5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196459.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2336"/>
    <dbReference type="EnsemblBacteria" id="BAD41321">
        <property type="protein sequence ID" value="BAD41321"/>
        <property type="gene ID" value="STH2336"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2336"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_3_0_9"/>
    <dbReference type="OMA" id="LRWGYRR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="244" mass="27519" checksum="3C3D606C9298A5D4" modified="2004-10-11" version="1">MAGGGAAGRIERRGRQSRRTERALRILDAAASLVERYGYSKTTMDDVAREAGVAKGTLYLHWNTREELFGAVLRREQILLTRSFLDRMRAQKQPLTVHALFKQAALGLLERPLVRAVFTRDVEVVGNLLRREQRDHPEQKELFHLLLDFLGRSNLMRRDLTPQAQAFLVSAIFLGFFLMVSWVPDQAEMSDEQVAGLMADTVVAALHLDGQATPREEAEAAEDVLRHLEELVAREEAAFQAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M82</accession>
    <name>Q67M82_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196351.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2226"/>
    <dbReference type="EnsemblBacteria" id="BAD41211">
        <property type="protein sequence ID" value="BAD41211"/>
        <property type="gene ID" value="STH2226"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2226"/>
    <dbReference type="HOGENOM" id="CLU_553129_0_0_9"/>
    <dbReference type="OMA" id="DNAQAFM"/>
    <dbReference type="OrthoDB" id="764010at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013493">
        <property type="entry name" value="CHP02677"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09660">
        <property type="entry name" value="DUF2397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02677">
        <property type="entry name" value="TIGR02677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="493" mass="56445" checksum="023580D438E0015F" modified="2004-10-11" version="1">MPPRAVTAEMPEANYLIGHPDGKYYRLLMRCFYERSLSHITYVRTDDLVAFVQQYLPYDDATCRQHLDQMEKWGLVTLIPEQSKPANLMELRQKPRVYQASRIALRLEALRAELEQEEGAASLDPAALDLLVQRVRELADFIEQDGLHRDGAAPEAHRLWAGVYESFGAFSRRIREYLEDLPRHRPREVLDYEAFRAYRDLLIPYLQDYARRLFDRREQLRMRLRVLARSKELLATTAAVVEAQQVRADGSRPDFDRQKERFLREIDALIGYFGDQGDVDVLLERAQAWVSEITRHARRLSEQHLGGSVREQTLLDLARRFSECTSLEQAESLAQVVFAATLPLHWRGEAPPPADKDPWEAEPVTVPLYAVRRGQRPRQQPEATADRTSEALQKMLSAKAERDRAAQELAELFGDESELDLGDLTVREPRQRQLLLRLLYKALSQQGPVGVGYRNWSVTAEVKADAPLGRLSAADGTATLPHVILRLHKGGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M89</accession>
    <name>Q67M89_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196344.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2219"/>
    <dbReference type="EnsemblBacteria" id="BAD41204">
        <property type="protein sequence ID" value="BAD41204"/>
        <property type="gene ID" value="STH2219"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2219"/>
    <dbReference type="eggNOG" id="COG2433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688726_0_0_9"/>
    <dbReference type="OrthoDB" id="1063332at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269265" evidence="1">
        <location>
            <begin position="31"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="211"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="296"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="394" mass="45720" checksum="5E3C24024C38CEC5" modified="2004-10-11" version="1">MQRGYARRARQLACLVLAALLLHTPVRTGAAEEMKPEVAEYPAENYEPGPGWPEGWTPPPPPEGMSEQMRAEVERILREHPDMYDRYDWWVWDPLPAWTNEYPWFWREDKMGCGTTLYVFAYPVPDTQLITRVFGRSFGWSRLYLKPCRYEPIQSITETRELSEEEKLAEERGIEYEYWMDKSHPGLGRDDGRASNGSPDHVFVYFNQGDASKRFDTPAYLDTTVNRVRVPIRFISEMMGAEVSWDQAKRQVTIYFPAVTREVVKAVPAPGYDYPDLFDPEAYLPNGHRFLLEKQTISTSERTIVLTVDHPAAIVDGMEIPLDAPPVIRNDRTMVPVRFIAEQMGAKVYWVGAEPIFRRDDGSMSGTYQVHIFTPFFPLFEYPSWYLETRAVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME8</accession>
    <name>Q67ME8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196285.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2160"/>
    <dbReference type="EnsemblBacteria" id="BAD41145">
        <property type="protein sequence ID" value="BAD41145"/>
        <property type="gene ID" value="STH2160"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2160"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_1_9"/>
    <dbReference type="OMA" id="HPCIMAP"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="7">
        <location>
            <begin position="121"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="459" mass="47338" checksum="5496501171F49320" modified="2004-10-11" version="1">MEITMPQLGESVTEGTINRWLVAPGDVVKRYQPIAEVITDKVNAEIPAPADGRILTLDVPEGSTVPVGARIATMEVAGEDAGQAPAPVGASAQAASQAAAPQGAPAVGGGSGAPDRASRGRYSPAVLRLAQEHGVDLSQVRGTGLGGRITRKDVEAFIAARAAGAPAAPPAAAAAPAGVAAPAPAGVAASAPAAPASGVAVAATSAPSVAAPTPSAPAAAPVQPAPGDRVIPVDPVRRRIAAKMVQAKHEAPHAWTMMQVDVTNLVKLREQAGPEFRARTGRPLSYVPFFIKAVVESLREYPILNSQWNGDEIVIRQDINISVAVATEDALAVPVIKHADRLSIAGLNEAVADLAERARAGRLTLDDVTGGTFTVNNTGAFGSFLSAPIINYPQAAILSFEKITKMPVVLENDAIAIRSMMNICLSLDHRILDGLVCGRFLQAVKRRLESYGPGTVSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK1</accession>
    <name>Q67MK1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2107</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2107"/>
    <dbReference type="EnsemblBacteria" id="BAD41092">
        <property type="protein sequence ID" value="BAD41092"/>
        <property type="gene ID" value="STH2107"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2107"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066398_0_0_9"/>
    <dbReference type="OMA" id="CSTYFKR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27001" checksum="37D9228F7CF513C1" modified="2004-10-11" version="1">MGLLGLLAILIFAAAMQGSRPTGFAPEVGAVIYAALAMFQLVLLILAVPGLAAGAIAGERERQTLDLLLVTRLSAAQVVVGKLLAATGFALLLMFASLPVFGLIFLFGGFSLSRLGLSAVVYVVTVLLLGAVSLYFSALYRRTQTAVVAAYGVVTTWTLLSPLFGALTYHVFFDEHPPLWGLIFAYTNPAFGLAAAAGQPLETMTRLYSPILTTDAAREAIWWKYCLIALGLIALLLWRTVRRVDPARAPAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML2</accession>
    <name>Q67ML2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome c oxidase subunit 2</fullName>
            <ecNumber evidence="17">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">STH2096</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 17">Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="17">Binds a copper A center.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="17"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2096"/>
    <dbReference type="EnsemblBacteria" id="BAD41081">
        <property type="protein sequence ID" value="BAD41081"/>
        <property type="gene ID" value="STH2096"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2096"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_1_1_9"/>
    <dbReference type="OMA" id="KMTGTYP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04213">
        <property type="entry name" value="CuRO_CcO_Caa3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034236">
        <property type="entry name" value="CuRO_CcO_Caa3_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014222">
        <property type="entry name" value="Cyt_c_oxidase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02866">
        <property type="entry name" value="CoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0186">Copper</keyword>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="20">
        <location>
            <begin position="25"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="19">
        <location>
            <begin position="123"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="21">
        <location>
            <begin position="248"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BAD41081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="342" mass="37295" checksum="AF772FE1996CEF89" modified="2004-10-11" version="1">MGMWIRQSGVRTCAAILLASVLLAGCSTLPQTPLDPKGPVAQMQTGLLLYTLYIAMGIGISVTAALLYVVFRFRARPGLTGVPVQIRGNHVLEIIWTVIPVLILVSVAFPTVEAAFSTAVPPEGAMTVRATGYRWWFAFEYPELGIVTANELRIPAGQPIRLELTSNDVIHSFWVPKLAGKTDMIPGRVNIAWIQADEPGVYLGQCAEFCGDSHANMRFQVRAMPPEQFDEWVRQRQAMAQGPSELSAVAARGRELFESNVGNCFACHAVDGTKAQGVVGPNLTDVGQRSTLAAGILENNYENLKAWVRNPADFKPGTTMPSHARLSEQDLDAIVQYLMSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN2</accession>
    <name>Q67MN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196201.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2076"/>
    <dbReference type="EnsemblBacteria" id="BAD41061">
        <property type="protein sequence ID" value="BAD41061"/>
        <property type="gene ID" value="STH2076"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2076"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089258_3_0_9"/>
    <dbReference type="OMA" id="HGYEIQQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="2">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="110"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="20180" checksum="A702EDC4C8775C93" modified="2004-10-11" version="1">MVRLVILGLLNQRPMSGYEIQQLHQVSRIDQWSDILPGSIYHALKKMAAEGLVALAGTEQTGMRTKAIFEITDAGRAEYRRVLREAWSQVPRSLPGDFYLLLSFAHDMPRADLQAALRELIARLEERLAQWEEGRAAKAAAVGDLPPPIRAAFDNGREHMEADLRFLRRLLEEFAESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV4</accession>
    <name>Q67MV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2004"/>
    <dbReference type="EnsemblBacteria" id="BAD40989">
        <property type="protein sequence ID" value="BAD40989"/>
        <property type="gene ID" value="STH2004"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2004"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2636742_0_0_9"/>
    <dbReference type="OrthoDB" id="2009475at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8710" checksum="5E1695075A623F69" modified="2004-10-11" version="1">MVVEASGARVLLEPEVYPVLKGSRLDYGPALKPPRFRVLRNPNTPQKCPCGRSFGRPYPGRKSPQCQAYRPMPWDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX2</accession>
    <name>Q67MX2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">STH1986</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + sn-glycerol 3-phosphate = a quinol + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196113.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1986"/>
    <dbReference type="EnsemblBacteria" id="BAD40971">
        <property type="protein sequence ID" value="BAD40971"/>
        <property type="gene ID" value="STH1986"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1986"/>
    <dbReference type="eggNOG" id="COG0578">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015740_0_1_9"/>
    <dbReference type="OMA" id="VYKGGPH"/>
    <dbReference type="OrthoDB" id="360065at2"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00673"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052591">
        <property type="term" value="F:sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017752">
        <property type="entry name" value="G3P_DH_GlpA_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="PTHR11985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03377">
        <property type="entry name" value="glycerol3P_GlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="13">
        <location>
            <begin position="5"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Fer2_BFD" evidence="14">
        <location>
            <begin position="424"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD40971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="545" mass="57551" checksum="090D4B0D8729D666" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY0</accession>
    <name>Q67MY0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1978"/>
    <dbReference type="EnsemblBacteria" id="BAD40963">
        <property type="protein sequence ID" value="BAD40963"/>
        <property type="gene ID" value="STH1978"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1978"/>
    <dbReference type="eggNOG" id="ENOG5032T7S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1554518_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="19334" checksum="C10CA921A4E2499B" modified="2004-10-11" version="1">MQVMMRGDVDRFYRAIRREHPEPPPGPIRHLDHVRTIADYYARLSQQERLLMLAWARKEESGIGIIPLALSAIPFFGLIVSARLQEVLNRLPIWQVFVTWLLFGSLVLLGFYVHQSQKAWTALHITLLEQAIAAAEAAQSEPAQAGAPDGDPPQPSPEDARRLTFPPETGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N12</accession>
    <name>Q67N12_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-isopropylmalate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196073.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1946"/>
    <dbReference type="EnsemblBacteria" id="BAD40931">
        <property type="protein sequence ID" value="BAD40931"/>
        <property type="gene ID" value="STH1946"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1946"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_9"/>
    <dbReference type="OMA" id="DVRYEPQ"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="1">
        <location>
            <begin position="6"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="357" mass="38631" checksum="B5BF5EC231D5AF44" modified="2004-10-11" version="1">MAFVPTIVVLEGDQTGQELLEEAVRLLSPDVIGLPLHLVRYDLSLENRRATSNRVVYEAAAAMREHGYGLKAATITPEGRGDVGSPNAILRREIDGTVILRTGRPLPGVETIGGITAPIAVVRMATEDAYEAKEWREGEGDEERAFRTTYISARNCRATAEFAFRLARQMGALVFGGPKWTVSPTYEGLLKEAMDEAARRNPDVPYDPQLIDAAYALLIARATRPLVIPCLNRDGDILSDLVLALYGSIAGSESLLIAFDEQFNPRVVMAEAPHGTAPSLQGKNLANPLAMQLAAGALLKQMPDPEYQRAGAAIQEACLQAVAQGVRTADLGGTARTTEFTDEVIRRVKEKLAVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND4</accession>
    <name>Q67ND4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1824</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195952.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1824"/>
    <dbReference type="EnsemblBacteria" id="BAD40809">
        <property type="protein sequence ID" value="BAD40809"/>
        <property type="gene ID" value="STH1824"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1824"/>
    <dbReference type="eggNOG" id="COG1379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060249_0_0_9"/>
    <dbReference type="OMA" id="HAFTPWT"/>
    <dbReference type="OrthoDB" id="1095492at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="43368" checksum="F46CDEA76B9E5C5A" modified="2004-10-11" version="1">MPGSGAAEPLQVVYADLHVHLGWAGDPGGGVKIAAARDLTLANILAEARDRKGLGLVGVIDAATSGALQDLERLLAQGLVAERPGGGLAYGGLTLIPGAEVEVVHRGKPVHLLCYFGGLDGLRAFAEWQAPRVRNRHLSTQRHHGTTAADVVEAVADRGGVVIPAHIFTPHKATLAAAPAVSEVIPPELWASVPAVELGLSADTALADELPELSRFPFVTNSDAHSLGRIGREYNQLLLREPSFDEWLLALREEGGRRILANFGLDPRLGKYHRTFCEDCGRKVEGDPPVLRCPVDPGHRVVLGVLDRIRHYAAWQQGRFVRAERVRPPYVHQVPLSFVPGLGRKGMARLLAAFGTEMAVLHAAEEADLVAVVGERLARLVVLARQGRLAIESGGGGIYGRVSAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI2</accession>
    <name>Q67NI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoVS</name>
        <name evidence="1" type="ordered locus">STH1776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1776"/>
    <dbReference type="EnsemblBacteria" id="BAD40761">
        <property type="protein sequence ID" value="BAD40761"/>
        <property type="gene ID" value="STH1776"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1776"/>
    <dbReference type="eggNOG" id="COG2359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168904_1_0_9"/>
    <dbReference type="OMA" id="IGIEIAC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007347">
        <property type="entry name" value="SpoVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331">
        <property type="entry name" value="PTHR35331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04232">
        <property type="entry name" value="SpoVS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="84" mass="8558" checksum="80F194E96E3F8800" modified="2004-10-11" version="1">MLKVSAKSSPNAVAGALAGCLRERGSAEIQAIGAGALNQAVKAVAIARGFVAPSGVDLICIPAFTDLKIDGEERTAIKLIVEPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI4</accession>
    <name>AMPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cytosol aminopeptidase</fullName>
            <ecNumber evidence="1">3.4.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucine aminopeptidase</fullName>
            <shortName evidence="1">LAP</shortName>
            <ecNumber evidence="1">3.4.11.10</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Leucyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pepA</name>
        <name type="ordered locus">STH1774</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.</text>
            <dbReference type="EC" id="3.4.11.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="1"/>
    <dbReference type="EC" id="3.4.11.10" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195902.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NI4"/>
    <dbReference type="SMR" id="Q67NI4"/>
    <dbReference type="STRING" id="292459.STH1774"/>
    <dbReference type="EnsemblBacteria" id="BAD40759">
        <property type="protein sequence ID" value="BAD40759"/>
        <property type="gene ID" value="STH1774"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1774"/>
    <dbReference type="eggNOG" id="COG0260">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013734_2_2_9"/>
    <dbReference type="OMA" id="MKNTGPR"/>
    <dbReference type="OrthoDB" id="356206at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="PTHR11963"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000165801" description="Probable cytosol aminopeptidase">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00181"/>
        </source>
    </evidence>
    <sequence length="491" mass="51836" checksum="7BDEC9C36D1BD695" modified="2004-10-11" version="1">MKVEVTSQALTEVAVDVLILPLTEGTPAPADVNEALGGLCAQVVGADFKGEMGQTTLLYTGGRLPARKVLLLGLGKAEKVTNRSLRRAAGIGARAARKSGARSIAFALPGAVEMDEAAAARFITEGALHGLFRLPDWKSEKKPRPEVEAVHILGGERAREGAEHGRIVAEGVNLARALAWTPGNHLTAAKLAERAAEMCRENGIEVEVYDKKGCEELGLGLLLAVNQGSREEPRFIVMRYKGNGGQGPWLGLVGKGLTFDSGGISIKPTEGMWNMKMDMGGAAAVIGAMQAIARLKVKADVMAVIPSTDNMPDGGSYKPGDVVSGLSGKTVEIRSTDAEGRLILADGLAYAAKQGCRKIITASTLTGACNIALGPIRYALVANDDAWEEEVYRAGEAAGERGWKLPHDEEYYDLIKSSVADMINGTKNRAAGTVAGGLFLMKHVGDTPCVHLDIAAVAWKSGSDEYEDEGATGVGTRTFVEAARRFAEGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ7</accession>
    <name>Q67NJ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Peptide methionine sulfoxide reductase MsrA</fullName>
                <shortName evidence="9">Protein-methionine-S-oxide reductase</shortName>
                <ecNumber evidence="9">1.8.4.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">Peptide-methionine (S)-S-oxide reductase</fullName>
                <shortName evidence="9">Peptide Met(O) reductase</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">Peptide methionine sulfoxide reductase MsrB</fullName>
                <ecNumber evidence="8">1.8.4.12</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">Peptide-methionine (R)-S-oxide reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="9" type="primary">msrA</name>
        <name evidence="8" type="synonym">msrB</name>
        <name evidence="11" type="ordered locus">STH1761</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 8">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="9"/>
    <dbReference type="EC" id="1.8.4.12" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195889.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1761"/>
    <dbReference type="EnsemblBacteria" id="BAD40746">
        <property type="protein sequence ID" value="BAD40746"/>
        <property type="gene ID" value="STH1761"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1761"/>
    <dbReference type="eggNOG" id="COG0225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_1_0_9"/>
    <dbReference type="OMA" id="GGQFNDR"/>
    <dbReference type="OrthoDB" id="1554384at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01400">
        <property type="entry name" value="MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="PTHR10173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="SSF55068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00357">
        <property type="entry name" value="TIGR00357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MsrB" evidence="10">
        <location>
            <begin position="181"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="36941" checksum="FA09FBDD3A29FF65" modified="2004-10-11" version="1">MDEKRLELATFAGGCFWCMVSPFEEQPGIYKVVSGYTGGHKENPTYEEVCSGTTGHFEAVQITFDPDVFPYERLLDLFWQQIDPTDPDGQFYDRGPQYRTAIFYHNEEQRRLAEKSKAELEASGRFDKPIATLILPAGPFYPAEEYHQGYHRKNPFHYQRYRAASGRDDFIRKHWRDPERDAELRRRLTPIQYEVTQLGATERPFTGEYWNHFEEGIYVDVVSGEPLFSSRDKFDAGCGWPSFTRPLVPENIVERLDLSHGMVRTEVRSRGADSHLGHVFPDGPAPTGLRYCINSAALRFIPKEDLEKEGYGEFRKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU2</accession>
    <name>Q67NU2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">recX</name>
        <name evidence="7" type="ordered locus">STH1666</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Modulates RecA activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the RecX family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1666"/>
    <dbReference type="EnsemblBacteria" id="BAD40651">
        <property type="protein sequence ID" value="BAD40651"/>
        <property type="gene ID" value="STH1666"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1666"/>
    <dbReference type="eggNOG" id="COG2137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066607_4_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01114">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003783">
        <property type="entry name" value="Regulatory_RecX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602">
        <property type="entry name" value="PTHR33602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02631">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24219" checksum="A03E592B56FD02BC" modified="2004-10-11" version="1">MDGEFVLGLSLETILRCGLKVGLPVDGETLLAAYGMDLERQAWEAGLRLLAAAPRTRREVALRLGRRYPPETVERVLERLTAAGWLDDRAYAVGYVRSHPDYGSRRLLADLIRKGVDRENAAAAVASERGAEEAVEHARAVAEARLRRMTGVDRTTAARRLAGYLARRGFGPEAVRAAVQPLVAGLPDQAPARRTRGLQRRTSLGRRGDGKADRGGAGGDGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV5</accession>
    <name>Q67NV5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1653"/>
    <dbReference type="EnsemblBacteria" id="BAD40638">
        <property type="protein sequence ID" value="BAD40638"/>
        <property type="gene ID" value="STH1653"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1653"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068415_0_0_9"/>
    <dbReference type="OMA" id="LGSMPNY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="36001" checksum="DBC7DAA4961F07BE" modified="2004-10-11" version="1">MSVLLGGCAGRPAGSLGAAPVGEAEDGAEAPKDYGEIRLGYQFGTGYLPVEVMVEHRLIEKRLGNVKVTRQQLGGGGALTEAVLAGSTDVVFMGLGPFFVGWGKGIDWKIAAAMQDMPIGLNSAKPGATSLRDIGPEDRIALPGINSIQHVMLAMEAQKQLGDPRALDELLVAMPHPEGERALLAGSEITFHYTAPPYLQRERQQPGIAKIVDSYETMGQKHTFNVMVVPARFKEENPELYQALVDAYTEATEWVRANPAEAADLMIRLGDRTDRDELIAQITHPDVVWTIEPHGLMKIVTFMKEAGFLDKAPADWREVTWENLHHLNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV6</accession>
    <name>Q67NV6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1652</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195781.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1652"/>
    <dbReference type="EnsemblBacteria" id="BAD40637">
        <property type="protein sequence ID" value="BAD40637"/>
        <property type="gene ID" value="STH1652"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1652"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_9"/>
    <dbReference type="OMA" id="IFICSVW"/>
    <dbReference type="OrthoDB" id="1531811at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="55"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="27621" checksum="01DF136C16F00EA4" modified="2004-10-11" version="1">MRNGGNKLVVVLVLLALWEGYARAADVNPLLVPAPSRVLLAFGEYIRDGSLLRHTLHTLQMVLTGMSIGILTAFCLTALATAGRWGRDLLLTLTTIMNPLPAIALLPVALLWLGIGPRSLVFVIANSVVWSLALNMHTGFETVPLTWRQAGQNLGLRGWGLIRHVYLPAALPYILTGVKIAWSFGWRTVIAAELVFGASGQAGGLGWMINVERYNLNTAGVFCGLVTIVALGLLAEALFEVLERRTIRRWGMSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX1</accession>
    <name>Q67NX1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195766.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1637"/>
    <dbReference type="EnsemblBacteria" id="BAD40622">
        <property type="protein sequence ID" value="BAD40622"/>
        <property type="gene ID" value="STH1637"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1637"/>
    <dbReference type="HOGENOM" id="CLU_1474450_0_0_9"/>
    <dbReference type="OMA" id="SIYQERY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="183" mass="19201" checksum="6B72B2BD96DF5510" modified="2004-10-11" version="1">MNLRQLRWPVVLVTLAVTLAGLFGAGQLVRSQTIDQPLVTALAGVDGLAAYHLAAVGGVSEITIEPAPGASLRKVYGEVDRRVRQILKDGQYVIAVAGSGAGELEPLVERLNLFVQEAVATGAFTGMADRIAAEAAAAGARAHMAVDDRRVYLTVWQADAYAYRVVERPAWPPAAPQGGGTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P24</accession>
    <name>Q67P24_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1584</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195713.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1584"/>
    <dbReference type="EnsemblBacteria" id="BAD40569">
        <property type="protein sequence ID" value="BAD40569"/>
        <property type="gene ID" value="STH1584"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1584"/>
    <dbReference type="eggNOG" id="COG1426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047530_3_0_9"/>
    <dbReference type="OrthoDB" id="2071222at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="313" mass="33961" checksum="0160D79083C9845B" modified="2004-10-11" version="1">MEEIGRRLKAARLAKGLTLEQVEEETRIRKKYLDALESGRTVLIPGEVYVKGFLRSYGNFLGLDGEGLVEEYKALKARPAAEDGAGNGGPAEAPAREPEAAIAASAPRASAEPRQPAFATPPRRSSRSRPKRRRNGPGPGVYFLRRLMVALILILPLAAAGWWFWGRQAAAPPPQEPGQVAQEPGTTAQQPEPEPEPEPEPEPEPEQPPASGQPVPAKPVITVGAPQGDEVDITITASEVQLEMDFTQGFPWLEVYSGGETLYWSKASGPLSFTGKDFRIRIGFVAGFQLALNGEPVDHKLEGGPYWINIKTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB6</accession>
    <name>EFTS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor Ts</fullName>
            <shortName evidence="1">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsf</name>
        <name type="ordered locus">STH1492</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195622.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PB6"/>
    <dbReference type="SMR" id="Q67PB6"/>
    <dbReference type="STRING" id="292459.STH1492"/>
    <dbReference type="EnsemblBacteria" id="BAD40477">
        <property type="protein sequence ID" value="BAD40477"/>
        <property type="gene ID" value="STH1492"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1492"/>
    <dbReference type="eggNOG" id="COG0264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047155_0_2_9"/>
    <dbReference type="OMA" id="DAGMMDC"/>
    <dbReference type="OrthoDB" id="1405357at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000161215" description="Elongation factor Ts">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="1">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <sequence length="304" mass="33403" checksum="81702109F00D3736" modified="2004-10-11" version="1">MAEITAKMVAELRARTGAGMMDCKKALMETGGDFDKAVDWLREKGLAAAAKKAGRVAAEGRVHAIVEDGARHGVLVEVNCETDFVARGEAFINLCDHVARVILQARPASLEALQEALGDTVKEAVAKIGENIQVRRFERYEVADAGRVHAYIHGDGRVGVLIELTTATPEVAAHPEVEALCHELALQIASMRAQYVRPEDVPAQVIEHEREILKAQAINEGKKPEIAEKMVAGRIQKFFQEVCLLEQEWVKDSKKTVGALVKEVAGKVGGEITVKRFVRYEKGEGIEKRQDDFAEEVMRQAGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC3</accession>
    <name>Q67PC3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative flagellar protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195615.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1485"/>
    <dbReference type="EnsemblBacteria" id="BAD40470">
        <property type="protein sequence ID" value="BAD40470"/>
        <property type="gene ID" value="STH1485"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1485"/>
    <dbReference type="eggNOG" id="COG1582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173020_1_0_9"/>
    <dbReference type="OMA" id="HQIESIE"/>
    <dbReference type="OrthoDB" id="2007051at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009384">
        <property type="entry name" value="SwrD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39185">
        <property type="entry name" value="PTHR39185"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06289">
        <property type="entry name" value="FlbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0966">Cell projection</keyword>
    <keyword evidence="1" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8254" checksum="03C00FD824384834" modified="2004-10-11" version="1">MIKLRRFRSKGGEFVVNADLIETVEATPDTVVTLTTGHKLIVEESVDEVIRKVVEFKRACHTPLLGHSDPHAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC5</accession>
    <name>Q67PC5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine recombinase XerC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">xerC</name>
        <name evidence="13" type="ordered locus">STH1483</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 'phage' integrase family. XerC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1483"/>
    <dbReference type="EnsemblBacteria" id="BAD40468">
        <property type="protein sequence ID" value="BAD40468"/>
        <property type="gene ID" value="STH1483"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1483"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_6_9"/>
    <dbReference type="OMA" id="QAFWYLI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01808">
        <property type="entry name" value="Recomb_XerC_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023009">
        <property type="entry name" value="Tyrosine_recombinase_XerC/XerD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0229">DNA integration</keyword>
    <keyword evidence="7 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="12">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="11">
        <location>
            <begin position="149"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="9">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="39728" checksum="57502654449C88AC" modified="2004-10-11" version="1">MGRVGAQPQGDSLGPEVAGFLASLSVEKSASQHTLRSYRLDLAQFYAWLASGGTTAPVVGGRGSGRTRRHDFAAQQEEPVRPVGLDELRAVNHLTIRSYLAFLQRQEFSRRTIARKLSCLRSFYKYLVRADLVPTNPVAGVHTPKLERRLPVFLDEEEIGGLLSQPDTHTPLGLRDRALLELLYATGLRVGELVALNRGDIDYSEGWVIVWGKGRKERAVPVGSEALQALGHYLRDGWPVLRARAPAAEQSLPEAKQPLFLNKLGGRLSDRSVRRLLDRYVSQLALSRQISPHKIRHTFATHMLNHGADLRAVQEMLGHASLSTTQIYTHVTTQRLRTEYLRAHPRQRRAAPPDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF2</accession>
    <name>Q67PF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1456</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1456"/>
    <dbReference type="EnsemblBacteria" id="BAD40441">
        <property type="protein sequence ID" value="BAD40441"/>
        <property type="gene ID" value="STH1456"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1456"/>
    <dbReference type="HOGENOM" id="CLU_3104663_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="51" mass="5653" checksum="F6104A3BD385022B" modified="2004-10-11" version="1">MGKEDLRLDESGRPVEEDGGHRLPGWVYGLLLALLVVILLGVESILNWLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI2</accession>
    <name>Q67PI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1426</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195556.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1426"/>
    <dbReference type="EnsemblBacteria" id="BAD40411">
        <property type="protein sequence ID" value="BAD40411"/>
        <property type="gene ID" value="STH1426"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1426"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_9"/>
    <dbReference type="OMA" id="RWPATVQ"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="24"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="340"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="373"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="582" mass="63780" checksum="935652A6C058EDDF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ9</accession>
    <name>TRPD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">STH1409</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195539.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PJ9"/>
    <dbReference type="SMR" id="Q67PJ9"/>
    <dbReference type="STRING" id="292459.STH1409"/>
    <dbReference type="EnsemblBacteria" id="BAD40394">
        <property type="protein sequence ID" value="BAD40394"/>
        <property type="gene ID" value="STH1409"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1409"/>
    <dbReference type="eggNOG" id="COG0547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_9"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="OrthoDB" id="1238435at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000227192" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <sequence length="341" mass="36353" checksum="9DBCB794E10C7788" modified="2004-10-11" version="1">MKALLRQVAEGKDLTEAQAEAAMTLMMTGEATPAQVAAFLMALRIKGETVAEITGAARVMRERAIRIHHSRPLVVDTCGTGGDGSHTFNISTTAAFVVAGAGVAVAKHGNRAATSLTGSADVLEALGIHLDLTPEEVGRCIDEVGIGFLYAPALHTSMKHVAPVRREIGLRNIFNLLGPLTNPAMAQAQLMGVYDPNLTEPLARVLGNLGVKHALVVHGTDGVDEISISAPTVVSEMRDGFVHTYRVVPEDVGLSRAPREYIRGGTKEENARITESVLSGEPGPRRDVVLLNAAAALLAADRVRTLREGVELAAQSIDSGEARRVLERMREFTHRLRAESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK0</accession>
    <name>Q67PK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anthranilate synthetase component II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195538.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1408"/>
    <dbReference type="MEROPS" id="C26.955"/>
    <dbReference type="EnsemblBacteria" id="BAD40393">
        <property type="protein sequence ID" value="BAD40393"/>
        <property type="gene ID" value="STH1408"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1408"/>
    <dbReference type="eggNOG" id="COG0512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_9"/>
    <dbReference type="OMA" id="HQVVIYR"/>
    <dbReference type="OrthoDB" id="1238435at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="20715" checksum="7178DA2B9493BB56" modified="2004-10-11" version="1">MIVVIDNYDSFTYNLVQCLGELGAAQAVFRNDQITVDQVAALRPEAVVISPGPCTPDEAGISLELIRRLGPTTPILGVCLGHQAIGQAYGGRVVPAPSLMHGKTSLVRHSGEGLFVGLPNPFTAGRYHSLVVDRESLPDDLVVTAETEDGLVMAVQHRVHPVYGVQFHPESILTECGRQLLQNFLRLAQPAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK6</accession>
    <name>Q67PK6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1402"/>
    <dbReference type="EnsemblBacteria" id="BAD40387">
        <property type="protein sequence ID" value="BAD40387"/>
        <property type="gene ID" value="STH1402"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1402"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3258846_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="42" mass="4763" checksum="1F1A2D19929404E0" modified="2004-10-11" version="1">MEAFAAAVGARCLWLETSNLDDPAIQFYRRLGFRWCGLDVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM9</accession>
    <name>Q67PM9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MurNAc-LAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195509.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1379"/>
    <dbReference type="EnsemblBacteria" id="BAD40364">
        <property type="protein sequence ID" value="BAD40364"/>
        <property type="gene ID" value="STH1379"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1379"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460719_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MurNAc-LAA" evidence="2">
        <location>
            <begin position="36"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="592" mass="60503" checksum="72372D1FA8024E2D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW8</accession>
    <name>Q67PW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1290"/>
    <dbReference type="EnsemblBacteria" id="BAD40275">
        <property type="protein sequence ID" value="BAD40275"/>
        <property type="gene ID" value="STH1290"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1290"/>
    <dbReference type="HOGENOM" id="CLU_3066975_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="53" mass="5973" checksum="982264825FC73545" modified="2004-10-11" version="1">MKTSYPAPAKELDSVSPLFRKALAYVQHDAEDIYVLSAKYGLVTLDQPLEPYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q25</accession>
    <name>Q67Q25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1233</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1233"/>
    <dbReference type="EnsemblBacteria" id="BAD40218">
        <property type="protein sequence ID" value="BAD40218"/>
        <property type="gene ID" value="STH1233"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1233"/>
    <dbReference type="eggNOG" id="COG1079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040769_1_2_9"/>
    <dbReference type="OMA" id="MIFGNWM"/>
    <dbReference type="OrthoDB" id="1215743at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="30967" checksum="A89978FD402FCCFD" modified="2004-10-11" version="1">MSVGFGMILITFILATLRSTTPLLLAALGGVFSERSGVVNIALEGIMLTGAWAAAYFSFLTGSAWLGLLGAIVVGMLMAGIHGVISIKYHADQVVSGTAINILAVGFTEFMLNRIWGQAGSSPEVATLPSFGGLSLMIYLAFILAFVSWVALYRTPWGLRLRSVGEHPLAADTLGINVYQMRYAGVLLSGALGGMAGAALSIGLLSRFVDGMSAGKGFIALAAMIFGKWHPIGAVGATLLFGASQSLSTIFQLMGISGIPTQFFNMLPYVLTMLILAGFVGKSVAPAADGVPYKKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q44</accession>
    <name>Q67Q44_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diacylglycerol/lipid kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195345.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1214"/>
    <dbReference type="EnsemblBacteria" id="BAD40199">
        <property type="protein sequence ID" value="BAD40199"/>
        <property type="gene ID" value="STH1214"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1214"/>
    <dbReference type="eggNOG" id="COG1597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045532_0_2_9"/>
    <dbReference type="OMA" id="NDFAKYW"/>
    <dbReference type="OrthoDB" id="869726at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005218">
        <property type="entry name" value="Diacylglycerol/lipid_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00147">
        <property type="entry name" value="TIGR00147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="292" mass="31716" checksum="C58B5442342896BE" modified="2004-10-11" version="1">MPRVCFIVNPIAGRGQALERWRQIEPLAARLGEYGVKFTERPGHGTDLARLAIQEGYDRVVSIGGDGTLNEVGNGLVGTNAALAVIPAGRGNDWVRTAGVPTDAAEGCRLAFGGRVARMDVGLAHGYRYFFNAAGFGFDAEVCARVNTYGQRFPKFSYVRGVFDTLFHFTGVPVDVEIDGERRRLNRVLLLEVGIGRYFGGGMQVFPQADIADGLFEIAWGEDLGRLELIRLVSLIYSGRHVGHPKVRMARGRKLTADSPEKVVIQLDGEVVGHLPVTFEILPGALNVVLPG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q97</accession>
    <name>RUVB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvB</name>
        <name type="ordered locus">STH1161</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195292.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q97"/>
    <dbReference type="SMR" id="Q67Q97"/>
    <dbReference type="STRING" id="292459.STH1161"/>
    <dbReference type="EnsemblBacteria" id="BAD40146">
        <property type="protein sequence ID" value="BAD40146"/>
        <property type="gene ID" value="STH1161"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1161"/>
    <dbReference type="eggNOG" id="COG2255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055599_1_0_9"/>
    <dbReference type="OMA" id="IHRMSRP"/>
    <dbReference type="OrthoDB" id="874197at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000165613" description="Holliday junction ATP-dependent DNA helicase RuvB">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <sequence length="347" mass="38736" checksum="E423DC81C3ECA81F" modified="2004-10-11" version="1">MSEERFVSGHRRPEDARIEETLRPQRLADYPGQERVKEQLSIYIAAAKKRGEPLDHVLLYGPPGLGKTTLAHIIAHEMGVNLRITSGPAISHQGDLAAILTQLSEGDVLFVDEIHRLNRLVEETLYPAMEDFALDIILGKGPAARTLRLDLPRFTLIGATTRYGALTSPLRDRFGVVLQLQFYSEEELTRILMRAARILNVPLDPGGAREIARRSRGTPRVANRLLRRLRDYAQVRADGVITRDVADAGLRMMGVDAMGLDTVDHKVLLTIIHHYGGGPVGLDTIAAATSEEPETIEDVYEPYLMQIGFLQRTPRGRVATRAAYEYLNIPYTDHPDEPEGPVQPRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP4</accession>
    <name>Q67QP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetolactate synthase-like TPP-requiring enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1014"/>
    <dbReference type="EnsemblBacteria" id="BAD39999">
        <property type="protein sequence ID" value="BAD39999"/>
        <property type="gene ID" value="STH1014"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1014"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_3_9"/>
    <dbReference type="OMA" id="PGPYGCL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="12"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="199"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="388"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="551" mass="58735" checksum="8F6D32615600E965" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP5</accession>
    <name>Q67QP5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1013"/>
    <dbReference type="EnsemblBacteria" id="BAD39998">
        <property type="protein sequence ID" value="BAD39998"/>
        <property type="gene ID" value="STH1013"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1013"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_0_2_9"/>
    <dbReference type="OMA" id="QHIEHAT"/>
    <dbReference type="OrthoDB" id="1701876at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014132">
        <property type="entry name" value="PdaB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02764">
        <property type="entry name" value="spore_ybaN_pdaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269331" evidence="1">
        <location>
            <begin position="34"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="63"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28920" checksum="7E5CD867AC00E9C9" modified="2004-10-11" version="1">MKMHRTPTRRRALLLAAVLMGAALALPAGTARARVTERNDAAPVSASLQPTLRPIYSARTEDKVVALTFDISWGNQMAPKVLEVLRNEKVPATFFLSGPWAKNHAELVKQIQADGHEIESHGHAHVDLNTLGREGAARNIAAAHAILKELTGRDPTYIRPPNGAFNEASVQAAKDLGYATVIWSVDSLDWKNPGVDRIVNRTVRLAHPGAIILMHASDSCKQTDQALPAIIRSLREQGYRFLLLDDLIRTYGVDMNGHIRVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ2</accession>
    <name>Q67QQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1006"/>
    <dbReference type="EnsemblBacteria" id="BAD39991">
        <property type="protein sequence ID" value="BAD39991"/>
        <property type="gene ID" value="STH1006"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1006"/>
    <dbReference type="HOGENOM" id="CLU_3277682_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="41" mass="4668" checksum="7090F37BD3B235C1" modified="2004-10-11" version="1">MDGVVIRRQKVVAFDNDVVYTVDRNGLARATRIDQIDSVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ8</accession>
    <name>Q67QQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195132.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1000"/>
    <dbReference type="EnsemblBacteria" id="BAD39985">
        <property type="protein sequence ID" value="BAD39985"/>
        <property type="gene ID" value="STH1000"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1000"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_9"/>
    <dbReference type="OMA" id="GPYTYGR"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="42859" checksum="09B2E648E7165728" modified="2004-10-11" version="1">MERPAPVPSDRAETLAAHAGDDPFRFLGAAAPPIFETSTFVFRTVADALAAFADEQEHYVYTRRRNPTVRVVEEKLAALEGAAECRLFASGMAAISAAILSCVGAGDHVVCVDTAYGPARAFITGYLSRFGISATLVDGCDPAEWEAAVRPNTRLFYLESPSSGLMKLQDLAAVTALARRHGIATICDNSYATMLLQRPLELGVDLVVYSCSKYFGGHSDVLAGAVLGPAERIRRIGDAEQPLLGGVVGPFEAWLIGRGLRTLPLRLRQHQQSAGRVAEFLAGHPRVARVHYPGHPSHPQHGLALRQMRGASGLLSFELDTGDLKQVSRFVDALRLFRLGVSWGGYESLVWLPVIGAMHRAPEEQWPNPGLVRIHVGLEDVDDLLEDLDRALRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU5</accession>
    <name>Q67QU5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH963"/>
    <dbReference type="EnsemblBacteria" id="BAD39948">
        <property type="protein sequence ID" value="BAD39948"/>
        <property type="gene ID" value="STH963"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH963"/>
    <dbReference type="eggNOG" id="COG1252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_7_2_9"/>
    <dbReference type="OMA" id="KWILVEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="407" mass="43614" checksum="6FA94BBD60EF5A7D" modified="2004-10-11" version="1">MDSRVRVVVLGAGYAGLTCFLELQDRLARGHDLVLVSRDRHHWFTTELHTYAAGHDADAVRIPLRRLVRPPGRLIIDHVSALRPAARQVELKLSGTLAYDLLVFALGSEPEYFGLPGIPRHALTIGSPPAAREVRERVRALAAREGPPGHVLVVGGGLTGVELAAELADEYPGRLRVTILEAAPEIMAGFPPDLVQVARRILEGKGIRILAGSPIVTADASCVHLQGGGEVPYDLLVWAGGVRGHSLLARAGLALGGRGRARVDAFLRSVIDDRIYVIGDSAAFADPASGREIPPTGQAAVQMGRAAAENILRRLGGRPERPFAFRMRGQFASLGHHEGVGVLGEAPMAGLPAMAVKHFVEGLHAWEVGGGVMPLVRRLVAAPVGYLRGRRLRTFTRQPRPVSEQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV7</accession>
    <name>Q67QV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PTS system cellobiose-specific IIB component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH951</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH951"/>
    <dbReference type="EnsemblBacteria" id="BAD39936">
        <property type="protein sequence ID" value="BAD39936"/>
        <property type="gene ID" value="STH951"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH951"/>
    <dbReference type="eggNOG" id="COG1440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147323_0_0_9"/>
    <dbReference type="OMA" id="CISKTAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003501">
        <property type="entry name" value="PTS_EIIB_2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013012">
        <property type="entry name" value="PTS_EIIB_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02302">
        <property type="entry name" value="PTS_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51100">
        <property type="entry name" value="PTS_EIIB_TYPE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIB type-3" evidence="4">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphocysteine; by EIIA" evidence="3">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="100" mass="10902" checksum="310FE4A80D0BFC70" modified="2004-10-11" version="1">MFILLACGMGASSGFLAQSMRKAAKSRGIEATIKAVSDTQIDDYLNDIDVLLIGPHLKHRFRDIESKAAPYQVKVVLVDERSYAMLDGESVLNQILGSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX4</accession>
    <name>Q67QX4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH934</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195066.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH934"/>
    <dbReference type="EnsemblBacteria" id="BAD39919">
        <property type="protein sequence ID" value="BAD39919"/>
        <property type="gene ID" value="STH934"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH934"/>
    <dbReference type="eggNOG" id="COG1145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_11_0_9"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="10504" checksum="02E32F2B36A163A1" modified="2004-10-11" version="1">MAVKIIDTCISCGACEPECPNEAISPGDTIYVIDPDKCTECYGFYSESQCIAVCPVECIIPDEDHVDDVESLTAKFNRLHPGQEMKNTDSWTKPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R20</accession>
    <name>Q67R20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH888</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195020.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH888"/>
    <dbReference type="EnsemblBacteria" id="BAD39873">
        <property type="protein sequence ID" value="BAD39873"/>
        <property type="gene ID" value="STH888"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH888"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="26846" checksum="7ABD0F8F274D61CA" modified="2004-10-11" version="1">MLTYELRDVSLTLGRKRIFSGLDLKLEGRVIGLLGPNGAGKTSLMRLLATLLRPTRGQILLHGRDIVREPAYARTRIGYVPQHFRPPGDLTGRQVLQYLGALRDVVEAERIDRCLVQTGLSRVADQAAGAYSGGTLRRLALAQALLSDPQVLLMDEPTAGLDPEEQDRFQTLVRGLAERGAQVLISTHLLAEVSQLADTAVVLHEGRILFAGPAPDLLARTGAQRLQDAYLRLLRDPALQVVGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R37</accession>
    <name>Q67R37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39856">
        <property type="protein sequence ID" value="BAD39856"/>
        <property type="gene ID" value="STH871"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH871"/>
    <dbReference type="HOGENOM" id="CLU_097573_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="20997" checksum="50F281BFD06C3890" modified="2004-10-11" version="1">MGGGDGLGLVVTVIAAMGGAAVVMLVAWLVVGGLVRAGLVGAIVAYRRGEDVGFATFWSYATRYAGKMVLLGLIYGVILLVSAVVVIFPLIGQIVYLLWLPTAFVTLGIYPAYLIIHDGYGVVSAVKQGLRILERQTGQTVLGGLIMALFYLAGSLLCAVPIVNIVGALFVAILGQPLVYYFFVERFESEVRPQVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R73</accession>
    <name>Q67R73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39820">
        <property type="protein sequence ID" value="BAD39820"/>
        <property type="gene ID" value="STH835"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH835"/>
    <dbReference type="HOGENOM" id="CLU_1844090_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15343" checksum="BC398BA937EF541C" modified="2004-10-11" version="1">MIGMAQAEEALSSVAERARRALRDNEAAPPQPPAHVDSPRPVRSPAAGSPTELIAQRMQAIARVKELEPRLASARARAGELSQRLTSTPPDSVHYAELLRWLADVHVQLRQLEAEYEEAQHIIRNSAWVMQLLADPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R85</accession>
    <name>Q67R85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH823</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH823"/>
    <dbReference type="EnsemblBacteria" id="BAD39808">
        <property type="protein sequence ID" value="BAD39808"/>
        <property type="gene ID" value="STH823"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH823"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_2_9"/>
    <dbReference type="OMA" id="PIFANKY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="36924" checksum="DD1DE3A5E12341A7" modified="2004-10-11" version="1">MATVPNGSSSFAGGGRPAVTVNAVVSAALIVALYIVLSLLRQQGIIGTYWGMILDLALITTTSAIGLSLIYGFAGQFSLGHVAFYGIGGYVSAYITKAYGATTLNFVLGLAAGTLAAALIAFLIGLPILRLRSDYLGIATLGFGVIVKVFMDNSDKISKLLGGSMGMTGIPRLTTFPWVFWSAVLVILLARNLVHSSHGRACISVREDEIAADIMGIDTTRFKMLAFVLGCAMAGLAGGLYAHLYVYLHPKSFEFLKSFDVLLIVVLGGLGSLTGTVVAGMGVVFLQEVLRDVLGQQFLEWRGVVYALVLIVLMILRPQGLMGGWEFSLDRWLSRPSKGGKASGTAGMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE0</accession>
    <name>Q67RE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative class-III aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194901.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH768"/>
    <dbReference type="EnsemblBacteria" id="BAD39753">
        <property type="protein sequence ID" value="BAD39753"/>
        <property type="gene ID" value="STH768"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH768"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013318_0_0_9"/>
    <dbReference type="OMA" id="AGICADS"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="875" mass="93791" checksum="36025B8E5CCFA7CA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ6</accession>
    <name>Q67RJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Anaerobic dimethyl sulfoxide reductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dmsB2</name>
        <name evidence="11" type="ordered locus">STH712</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Electron transfer subunit of the terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194845.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH712"/>
    <dbReference type="EnsemblBacteria" id="BAD39697">
        <property type="protein sequence ID" value="BAD39697"/>
        <property type="gene ID" value="STH712"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH712"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_2_0_9"/>
    <dbReference type="OMA" id="MARMKFV"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014297">
        <property type="entry name" value="DMSO_DmsB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02951">
        <property type="entry name" value="DMSO_dmsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="47"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="185" mass="20563" checksum="2E1D61371C3D2DB0" modified="2004-10-11" version="1">MAQKGFYYDMTACVGCKACYIACKDKNDLPLGVMYRNVYTFEGGRYPKPWIFNISISCNHCSDPRCVTNCPTGASYKREEDGLVLVDQEACIGCQYCVWSCPYGARHYLEEQGVVGKCNGCVDLVERGQNPACVDACVMRALEFGDLDELKRRHPEAEVFTSWGAPGAELTGPSMLVTLKPQARR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL6</accession>
    <name>MURB1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylenolpyruvoylglucosamine reductase 1</fullName>
            <ecNumber evidence="1">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramate dehydrogenase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murB1</name>
        <name type="ordered locus">STH692</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurB family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RL6"/>
    <dbReference type="SMR" id="Q67RL6"/>
    <dbReference type="STRING" id="292459.STH692"/>
    <dbReference type="EnsemblBacteria" id="BAD39677">
        <property type="protein sequence ID" value="BAD39677"/>
        <property type="gene ID" value="STH692"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH692"/>
    <dbReference type="eggNOG" id="COG0812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035304_0_1_9"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224728" description="UDP-N-acetylenolpyruvoylglucosamine reductase 1">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <sequence length="361" mass="39241" checksum="184A9A946B6032CE" modified="2004-10-11" version="1">MDVHGAAYATHGKGVTGIAALDIQQGVSLRDYSTMRLGGWAAYLAHVRSPAEVEEGIAWAEARHLPVIMVGGGSNIIWRDEGFAGLVLVNRIPGFELADQGGHLLLTVGAGENWDSVVARAVAAGASGIERLSLIPGTAGATPVQNVGAYGQEIADVLVSVDAYDRQERRFVRIPAAECAFGYRRSRFNQADRGRFFITALTLRLLREPPRAPFYPALGRYLEERGLTHPTVQQVRDAVIAIRRAKLPDPAHVANCGSFFRNPIIPAPQAAELLRRYPDMPHWPVPGGGVKLAAGWLIDRAGFRGVADPETGMGTWPAQALVVVNHRASSTADLLRFKQKVQDEVRRRFGVTLEQEPELLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR6</accession>
    <name>Q67RR6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome D ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH642</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome ubiquinol oxidase subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH642"/>
    <dbReference type="EnsemblBacteria" id="BAD39627">
        <property type="protein sequence ID" value="BAD39627"/>
        <property type="gene ID" value="STH642"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH642"/>
    <dbReference type="eggNOG" id="COG1271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_3_9"/>
    <dbReference type="OMA" id="MAIAFWS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="PTHR30365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0349">Heme</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="445" mass="49749" checksum="BA97D2ED3C92248C" modified="2004-10-11" version="1">MDVTMLSRIQFALTVGFHFVFVPLSIGLIIFTCIMETLYVRTGDPKYKTLTKFWGRLFTINFLLGVVTGVAMEFQFGTNWSRYSEFMGDIFGSPLAVEALMAFFLESTMLGVWIFGWNKLSKKMHLLAGWLIALGTHLSAVWIIVANGFMQNPVGYVLRNNRAELVNFWDVLFNPYAWYTYVHTLFACYAVGAFFVLAVSAYHLLRKRNVELMQICVKYGTVFAIIATLGNAVTGHYNGRNVALRQPVKLAAMEAQWETERGAGFSLIVVPDEKNERNAVQAITIPGLTSWLSYGSTDAEVLGLKDVPEELRPPVALTFWSFRLMVGLGILMLALAIAAWWHHRKGTLPDRPLLLRFLLWSIPLPYIATNLGWMTAEVGRQPWTVYGLITTADSVSPVAPADVAISLGLVVVFYAILVGLDAYLLVRYAKAGPEAYDERQVAVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX5</accession>
    <name>Q67RX5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="4 9">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH583</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + 3-methyl-2-oxobutanoate + H(+) = (R)-N(6)-(S(8)-2-methylpropanoyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10489"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the BCKDHA family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="4 9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194717.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH583"/>
    <dbReference type="EnsemblBacteria" id="BAD39568">
        <property type="protein sequence ID" value="BAD39568"/>
        <property type="gene ID" value="STH583"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH583"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_9"/>
    <dbReference type="OMA" id="QDSIACT"/>
    <dbReference type="OrthoDB" id="1248201at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034616">
        <property type="entry name" value="BCKDH_E1-a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="PTHR43380:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="E1_dh" evidence="11">
        <location>
            <begin position="18"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="334" mass="35087" checksum="32FA65438FD43142" modified="2004-10-11" version="1">MLDHRAAGLTDEQALAIYRWMLLTRRLDERLGVLQRSGAIPLALSSRGHEAAQVGAALAFARGKDWWFPYYRDLGAVLVAGTTPLDVMLSSFGRAADPSSGGRQTPYNWGDRRLNIVARSAPVGVQIPQAVGVAQAAVRRGDPIVVYCSFGEGAASQGDFHEGLNWAALHRLPVIFFCQNNGWAISVPLSRQVAGGSVAARAAGYGIEGVSLDGTDPFAVHAAVRRAVVRARAGGGPALIEARVHRLDPHTCDDNHAAYRTGAELRAAREADPLPAVADYLRRRGLLTDAAAAAVERAVAAEIDAAEAAARAARPPAGEEALGPVTAPPAPAPP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S17</accession>
    <name>CRCB1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB1</name>
        <name type="ordered locus">STH541</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194675.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S17"/>
    <dbReference type="SMR" id="Q67S17"/>
    <dbReference type="STRING" id="292459.STH541"/>
    <dbReference type="EnsemblBacteria" id="BAD39526">
        <property type="protein sequence ID" value="BAD39526"/>
        <property type="gene ID" value="STH541"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH541"/>
    <dbReference type="eggNOG" id="COG0239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_2_3_9"/>
    <dbReference type="OMA" id="IWIPARF"/>
    <dbReference type="OrthoDB" id="1942863at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000110198" description="Putative fluoride ion transporter CrcB 1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="124" mass="12775" checksum="1681E3FB5984D5E0" modified="2004-10-11" version="1">MSWVLIGVAGAAGAVARLLVGAWIDGRPGGRAFPWGTFAVNIAGSLLLGLLTGLVVGRGWLAPEVKVVLGAGFLGAFTTFSTWLLDLHEALRRGDHRAAFVNAALSTGLGLLAAWLGLALGWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S18</accession>
    <name>Q67S18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative xenobiotic reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194674.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH540"/>
    <dbReference type="EnsemblBacteria" id="BAD39525">
        <property type="protein sequence ID" value="BAD39525"/>
        <property type="gene ID" value="STH540"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH540"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_2_0_9"/>
    <dbReference type="OMA" id="NQRRDQY"/>
    <dbReference type="OrthoDB" id="1033671at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="PTHR43303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="2">
        <location>
            <begin position="4"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="37993" checksum="61378E549017982E" modified="2004-10-11" version="1">MAHLFDPFTQKSVTLRNRIVMSPMCMYVAGADGVATDWHLVHYGTRAVGGAGLIITEATAVEPRGRISEGDLGLWNDQQAEALARIARFVHDQGVAFAIQLAHAGRKAWTPCKGQGPEPAVAPSALPFDTDWVTPQALDAEGIDRVVAAFGAAARRAGELGCDLVEIHAAHGYLLHEFLSPLTNRRTDEYGGPLVNRARLLRRVFEAVREAFPADRPVWVRISATDWVEGGWDIAESVELARMMKAWGVDLVDVSTGGNSPLQRIPSIGPGYQVAFAERIRREAGIATGAVGLITTPEQADSVVRTGQADVALLGRELLRNPYFPLAAAHRLGHEVTWPEPYVRGKFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S74</accession>
    <name>Q67S74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH484"/>
    <dbReference type="EnsemblBacteria" id="BAD39469">
        <property type="protein sequence ID" value="BAD39469"/>
        <property type="gene ID" value="STH484"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH484"/>
    <dbReference type="HOGENOM" id="CLU_2358632_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="10588" checksum="708EB0F3261991B3" modified="2004-10-11" version="1">MRQQAGNICRRCGRSSEELFQMLDNGGRWAVVAGACTCGHRWTEIHTAVPNLSAVRRWLRQGCQPPDGPVGPMQGGPWQTWRVEGLGPEPVHSPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK5</accession>
    <name>Q67SK5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative biotin synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH353</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194487.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH353"/>
    <dbReference type="EnsemblBacteria" id="BAD39338">
        <property type="protein sequence ID" value="BAD39338"/>
        <property type="gene ID" value="STH353"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH353"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_0_1_9"/>
    <dbReference type="OMA" id="IMGFNPY"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024021">
        <property type="entry name" value="FeFe-hyd_HydE_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034422">
        <property type="entry name" value="HydE/PylB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43726">
        <property type="entry name" value="PTHR43726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00348">
        <property type="entry name" value="FeFe_hydrogenase_maturase_(Hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03956">
        <property type="entry name" value="rSAM_HydE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="52"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="350" mass="38275" checksum="9C39601CD23B9E87" modified="2004-10-11" version="1">MIRQEVLALIARAEAGADLSRDELTCLLSLGGEEQKALFAAADRVRARVMGDGVHLRGLIEFSNICRMDCCYCGIRRSNRHVRRYRMTPEEIVAAAQGAWRLGYGSVVLQSGEGDALDPEELAGVVRQIKATTPLAVTLSVGERTREEYALWREAGADRFLLRHETADPELFARLRPGRRLEQRIQCLLWLRELGYQVGSGFMVGLPGQTVGTLADDILLLKRLNVEMAGIGPFIPAPHTPLADAAPGTVEQTLNAVACVRLSIQDAHVPATTALGSIHPRGRQLALACGANVIMPNVTPGKYREHYQLYPNKICLREEPEQCEPCVAALIVGEGRFVATGPGHSPRWTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW3</accession>
    <name>Q67SW3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194380.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH245"/>
    <dbReference type="EnsemblBacteria" id="BAD39230">
        <property type="protein sequence ID" value="BAD39230"/>
        <property type="gene ID" value="STH245"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH245"/>
    <dbReference type="eggNOG" id="COG1937">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130332_0_0_9"/>
    <dbReference type="OMA" id="CEQILIQ"/>
    <dbReference type="OrthoDB" id="1868412at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003735">
        <property type="entry name" value="Metal_Tscrpt_repr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038390">
        <property type="entry name" value="Metal_Tscrpt_repr_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33677">
        <property type="entry name" value="PTHR33677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02583">
        <property type="entry name" value="Trns_repr_metal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="10427" checksum="617680AE28A1121B" modified="2004-10-11" version="1">MNIREVNPALYEDLMRRLRRIEGQARGIQRMLEEGQDCEQILIQLGAMKAAINRVGMKAAACNMGLQVTEAIRRGEDPTAAVDEVIEQFIKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX5</accession>
    <name>Q67SX5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194368.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH233"/>
    <dbReference type="EnsemblBacteria" id="BAD39218">
        <property type="protein sequence ID" value="BAD39218"/>
        <property type="gene ID" value="STH233"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH233"/>
    <dbReference type="HOGENOM" id="CLU_2195596_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="108" mass="11726" checksum="E76CCB3B24B62A31" modified="2004-10-11" version="1">MLEGKEALLNACRKRNMRVCETPINLLESDVQAEGNLDGMEVALEMNVISGMPGESRGTLTFQGNLSSLLAVLARVWPVPPEQDEAVSPGRQSWPAPPRKVGKDTPPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY8</accession>
    <name>Q67SY8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194355.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH220"/>
    <dbReference type="EnsemblBacteria" id="BAD39205">
        <property type="protein sequence ID" value="BAD39205"/>
        <property type="gene ID" value="STH220"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH220"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084710_0_0_9"/>
    <dbReference type="OMA" id="VANIYRP"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="24145" checksum="71E324DFD2877740" modified="2004-10-11" version="1">MEKAARTLVVGHFGSGKTEVAVFFALSLCAEQKRPMLLDLDFITPYYRSRDVADQLAAAGVEVVRPAGDLWRSDLPVVAARAVQAMTAYDGPVVADIGGGEGARVLGSMRLGPGTYEALMVVNPYRPDTGSPEQVARYARWLEEIGRIRLTGLVNNANLGPLTRPEHVLEGLKQVEEAARLLDLPVCCTAVRADLVDALPGLKLLPLRLCMRPPWEAEPFALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T39</accession>
    <name>Q67T39_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH169"/>
    <dbReference type="EnsemblBacteria" id="BAD39154">
        <property type="protein sequence ID" value="BAD39154"/>
        <property type="gene ID" value="STH169"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH169"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="IKSWFYK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="25"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="125"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="24428" checksum="4C944B9509115342" modified="2004-10-11" version="1">MLDAELDLIARCQAGDRQAFEELFTFYRDDVFRFAYLVVRDANLAQDVVQETFLKVFRSIANFQFRSSFKSWLYRVAVNEAITLLRRRRIKEELDPAPGAIQEHQRDRVDRAWQPEEAVLDSEERRLLHVAIGRLDPVHRTVIVLKYFHEFSDTEIAAVIGCPPGTVKSRLHRARELLRHHMARQAGRPDLIALSYTRLSAKTPLVPCEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-02" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T82</accession>
    <name>FTSH2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent zinc metalloprotease FtsH 2</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsH2</name>
        <name type="ordered locus">STH126</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194261.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T82"/>
    <dbReference type="SMR" id="Q67T82"/>
    <dbReference type="STRING" id="292459.STH126"/>
    <dbReference type="EnsemblBacteria" id="BAD39111">
        <property type="protein sequence ID" value="BAD39111"/>
        <property type="gene ID" value="STH126"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH126"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_9"/>
    <dbReference type="OrthoDB" id="190468at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000400403" description="ATP-dependent zinc metalloprotease FtsH 2">
        <location>
            <begin position="1"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="34"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="124"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="192"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <sequence length="587" mass="64101" checksum="CE283F1C5819D44A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T96</accession>
    <name>Q67T96_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formate dehydrogenase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH112"/>
    <dbReference type="EnsemblBacteria" id="BAD39097">
        <property type="protein sequence ID" value="BAD39097"/>
        <property type="gene ID" value="STH112"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH112"/>
    <dbReference type="eggNOG" id="COG2864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091368_1_0_9"/>
    <dbReference type="OMA" id="TIMSMIF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="3">
        <location>
            <begin position="3"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="209" mass="23593" checksum="A8314CC185E27DCF" modified="2004-10-11" version="1">MYRFGLASRLAHWNHALTFLLLLFTGLGLVVRGISGLAGAETLRLFGQLHRLAAIPFTVLTIPILLIGARRATARWLREVFRFDRDDLRFFPAFVREFFGLKAESPPQGKFNAGEKVNSILQIVGWPTMAVTGWMLVYKTSFPPALMQWVILIHSGMAMLLGCAVLGHIYLATLMPGFREGLSGMLSGWVPAKWAKDHYRKWYREITGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE9</accession>
    <name>Q67TE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH59</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194194.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH59"/>
    <dbReference type="EnsemblBacteria" id="BAD39044">
        <property type="protein sequence ID" value="BAD39044"/>
        <property type="gene ID" value="STH59"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH59"/>
    <dbReference type="HOGENOM" id="CLU_1524373_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="19951" checksum="AFE3861677E25568" modified="2004-10-11" version="1">MRRSLRKIDSQEVQQYAQSEPLFTVTSLDEARQRLVDMGQDPARYCFYTWMRQGVRYYRVWEDQRLKEGIVKPAPFGQEAARLKRADAEKLYTVAKRFAGDRVEFAQRDGVLWVIDGETGKAKVGISVPVVGGGLIFYQGEWNEHQAAHFVNALLVRGQRPDAAFVEAEALAGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH2</accession>
    <name>Q67TH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH36</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194171.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH36"/>
    <dbReference type="EnsemblBacteria" id="BAD39021">
        <property type="protein sequence ID" value="BAD39021"/>
        <property type="gene ID" value="STH36"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH36"/>
    <dbReference type="eggNOG" id="ENOG502Z9AE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065107_0_0_9"/>
    <dbReference type="OMA" id="MIFVTSE"/>
    <dbReference type="OrthoDB" id="1482071at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="379" mass="38634" checksum="3AD7CD460430CD32" modified="2004-10-11" version="1">MTDRLHAGPADDLAAGLLRAVLGGRDRGRISLVGLSKNAGKTVTLNRLIRAAAGLGIPLGLLSTGRDGEPVDAVTELPKPRIWAPEGTLVATAAAGGPDGGSGAAGEDRERTARVAVLRETGITTPLGPVVIGRVVRSGEVLLVGPGSAARIAALLDELEAAGAALCLVDGSLDRRAAAAPAVTGRAILAAGAAYSASMDATVSQVRYLLELFDLPEAPPEWRHGARPPVCLLLPDGPPVAVPVPSALTDPAVVAEAAAAAPAGAVLSVTGALTGGLLLALLSRPCAHIPILVPDPTHVLADRGAWRRWRRQGGQVFVEKRVEIAAVTTNAYSPVGPSYDAAAFCARVAEVAGRPVVDLVAGIAHHLPADHPHRDPVHR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J29</accession>
    <name>RNPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component</fullName>
            <shortName evidence="1">RNase P protein</shortName>
            <shortName evidence="1">RNaseP protein</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnpA</name>
        <name type="ordered locus">STH3340</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RnpA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197451.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J29"/>
    <dbReference type="SMR" id="Q67J29"/>
    <dbReference type="STRING" id="292459.STH3340"/>
    <dbReference type="EnsemblBacteria" id="BAD42321">
        <property type="protein sequence ID" value="BAD42321"/>
        <property type="gene ID" value="STH3340"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3340"/>
    <dbReference type="eggNOG" id="COG0594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117179_9_0_9"/>
    <dbReference type="OMA" id="PEQKHFR"/>
    <dbReference type="OrthoDB" id="2014742at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020539">
        <property type="entry name" value="RNase_P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00648">
        <property type="entry name" value="RIBONUCLEASE_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000198548" description="Ribonuclease P protein component">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <sequence length="115" mass="13144" checksum="8B8B95FC42AFB68F" modified="2004-10-11" version="1">MKKAYRLKSRLAFQSVYAQGRSVANRAAVVHVLKQQAGTPTRVGFAAGRKLGKAVVRNRAKRRLREAVRLLWPRVRQGYYIVVIARQAALDMPFPELRQKVEELFERAGLLQREG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-11-08" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J34</accession>
    <name>MNMG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmG</name>
        <name evidence="1" type="synonym">gidA</name>
        <name type="ordered locus">STH3335</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197446.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J34"/>
    <dbReference type="SMR" id="Q67J34"/>
    <dbReference type="STRING" id="292459.STH3335"/>
    <dbReference type="EnsemblBacteria" id="BAD42316">
        <property type="protein sequence ID" value="BAD42316"/>
        <property type="gene ID" value="STH3335"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3335"/>
    <dbReference type="eggNOG" id="COG0445">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_9"/>
    <dbReference type="OMA" id="FRPGYAI"/>
    <dbReference type="OrthoDB" id="146811at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000117195" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG">
        <location>
            <begin position="1"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="272"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <sequence length="630" mass="69758" checksum="9796CA6FAA221A4A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J64</accession>
    <name>Q67J64_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YycI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3305"/>
    <dbReference type="EnsemblBacteria" id="BAD42286">
        <property type="protein sequence ID" value="BAD42286"/>
        <property type="gene ID" value="STH3305"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3305"/>
    <dbReference type="eggNOG" id="COG4853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086652_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018604">
        <property type="entry name" value="YycI-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09648">
        <property type="entry name" value="YycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YycI" evidence="1">
        <location>
            <begin position="145"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="29569" checksum="4982DAFB8EB83EDC" modified="2004-10-11" version="1">MVNLILAYSLWGPKGELDPGEPTARSQLVQLRTRLDERGLILPNAVTLPTTPHPMRFLRVEFRPNPTKAPPVDEPSEGAPAASRLRSRYDAAARETVLEPVDGAGGTPVDLSDRLLLRQVAEDFLRANGLFPHDVQLSGIFLRENGAMVEFVPTYDGLPVFSGYLRVYLSPQGVERVAHHWVEPVGFKPGAPKAVRAASEALLRLAGHLEPGDGQVRTIVDVRLGYYSGPSVTVPGAEEISAWETVPVWRITLDNGQVYYVNAFNGELES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J84</accession>
    <name>Q67J84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3285"/>
    <dbReference type="EnsemblBacteria" id="BAD42266">
        <property type="protein sequence ID" value="BAD42266"/>
        <property type="gene ID" value="STH3285"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3285"/>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135679_0_0_9"/>
    <dbReference type="OMA" id="EEGWIYA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014934">
        <property type="entry name" value="DUF1806"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036492">
        <property type="entry name" value="YojF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08830">
        <property type="entry name" value="DUF1806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89442">
        <property type="entry name" value="SSF89442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="17928" checksum="F65272732A419340" modified="2004-10-11" version="1">MTHPPASGGRGMRLRLLAKPGAAYRAPPVGASKTTIQRAFGRLEAGPMEPIDREVVQQRLESFVNTDVYMHLETTNGAYAGEAAGGNGMAVCAFVRNARVRFSRAQITGSGPYRVGLQLHSGEGWTYAEGLTDYEVDEQGRLLLAGHDANGCLAIALELSHTPFPMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA2</accession>
    <name>Q67JA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197379.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3267"/>
    <dbReference type="EnsemblBacteria" id="BAD42248">
        <property type="protein sequence ID" value="BAD42248"/>
        <property type="gene ID" value="STH3267"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3267"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_602572_0_0_9"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="2"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="60"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="454" mass="47384" checksum="99E75F0107281760" modified="2004-10-11" version="1">MVFYFVQPGETLFAIARRYRTTVHALVTANRLKDPNAISPGQALLIPRPGERPSPPPGGVVHRVRRGETALTLAARYGTTVKAIMLANQLAHPEFVEPGQRLVIPEPPEPGTDWPMLGRDPRRASDSPAAPASPPSHRWTRRAAASRGFCPSAPVTRYGLVYVGLGDGVYRALDQRTGAVRWRAGAPAARAGGGSGEGFGAAALPAPVVYDGLAYYCVPGGPLRAVDARKGAEIWQVAPADALSTPLAGDGIVYCGHEGGVVAVEAKTGALAWNARLDGGVAQPVALGDGRLFAATEAGTLWALDALTGEPLWRAEAAAGAPVFAELVVALGGQAFDALTGELIWRRTDLGGTPAVRGEAVCYPGRWVDLFTGRTVAEADGEAPPAGAPHLLAGDLHLWVTPEPALVAWDAARRELRWSVPLPAPAVAMAPADGWIFVTLADGSLAAYSTTASE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB0</accession>
    <name>KTHY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">STH3258</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197371.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JB0"/>
    <dbReference type="SMR" id="Q67JB0"/>
    <dbReference type="STRING" id="292459.STH3258"/>
    <dbReference type="EnsemblBacteria" id="BAD42240">
        <property type="protein sequence ID" value="BAD42240"/>
        <property type="gene ID" value="STH3258"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3258"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_0_2_9"/>
    <dbReference type="OMA" id="VMTREPG"/>
    <dbReference type="OrthoDB" id="1585072at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155355" description="Thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="214" mass="23977" checksum="17AD6F3127F977FB" modified="2004-10-11" version="1">MSVFISFEGVDGSGKSTQIRLLLQYLDEQSVPYVFTREPGGTPIAEQIRRVLLDPANRGMSVITEALLFAAARAEHVSRTIRPALEEGKVVICDRFVDSSLVYQGVAGGLPVEFLTQINEMATGALRPHRTIVLDLAPEVALARRTGEEADRIERQSREYHQLVREGYLDLARAEPRRVKVVDASRSVEEVQKDIRRLVEEVLPRRFRGAGTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB6</accession>
    <name>Q67JB6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="14">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">metS1</name>
        <name evidence="14" type="synonym">metG</name>
        <name evidence="19" type="ordered locus">STH3252</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="14"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197365.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3252"/>
    <dbReference type="EnsemblBacteria" id="BAD42234">
        <property type="protein sequence ID" value="BAD42234"/>
        <property type="gene ID" value="STH3252"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3252"/>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_9_4_9"/>
    <dbReference type="OMA" id="SDMHGTP"/>
    <dbReference type="OrthoDB" id="761140at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01228">
        <property type="entry name" value="Met_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="PTHR43326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="18">
        <location>
            <begin position="549"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="14">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="14">
        <location>
            <begin position="297"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAD42234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="651" mass="73724" checksum="40E135E28D6CCF67" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC1</accession>
    <name>Q67JC1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197360.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3247"/>
    <dbReference type="EnsemblBacteria" id="BAD42229">
        <property type="protein sequence ID" value="BAD42229"/>
        <property type="gene ID" value="STH3247"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3247"/>
    <dbReference type="eggNOG" id="COG4466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153735_1_0_9"/>
    <dbReference type="OMA" id="NIFVVKF"/>
    <dbReference type="OrthoDB" id="1971787at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009366">
        <property type="entry name" value="Protein_Veg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40026">
        <property type="entry name" value="PTHR40026"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06257">
        <property type="entry name" value="VEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037257">
        <property type="entry name" value="DUF1021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="92" mass="10324" checksum="97E9C0C9422ECFE4" modified="2004-10-11" version="1">MASRSVLVDIKRDLEAYVGSKIRLRANKGRKKIVEREGVLEGTYPNIFTVRLSEADSVRRVSYSYADLLTETVELVVCSASGTETKFEAHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG5</accession>
    <name>Q67JG5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3203"/>
    <dbReference type="EnsemblBacteria" id="BAD42185">
        <property type="protein sequence ID" value="BAD42185"/>
        <property type="gene ID" value="STH3203"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3203"/>
    <dbReference type="eggNOG" id="COG3694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071040_1_0_9"/>
    <dbReference type="OMA" id="KPADAQF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="27615" checksum="591A318EF7F5FD91" modified="2004-10-11" version="1">MQYRVNFFSHLIGIVLTYAGQFAALYWLTQRFTTVGGWRFEEIILLYALAILAWGFAVSLFWSFISFEDQVRMGSFDRALLRPINPMLTVISTQSPIAGMGQFVFSLAAFTFAVKTAGIRLTALQLGYLVLTAAGGAMILGAAMMTVATMAFWTTRTYTFYWSLVYPARQLINYPVSIYHRAIQFVLTVLVPFAFINYFPAHVLLGRVQELPLPLLAWATPAVGVAAIGLAYGFWKFGTRRYTSTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK4</accession>
    <name>Q67JK4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aconitate hydratase</fullName>
            <shortName evidence="9">Aconitase</shortName>
            <ecNumber evidence="9">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH3164</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197277.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3164"/>
    <dbReference type="EnsemblBacteria" id="BAD42146">
        <property type="protein sequence ID" value="BAD42146"/>
        <property type="gene ID" value="STH3164"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3164"/>
    <dbReference type="eggNOG" id="COG1048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_9"/>
    <dbReference type="OMA" id="NGGIMQY"/>
    <dbReference type="OrthoDB" id="363064at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="PTHR11670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase" evidence="10">
        <location>
            <begin position="75"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="11">
        <location>
            <begin position="686"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="890" mass="97127" checksum="45F61DB283717925" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN3</accession>
    <name>Q67JN3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UVR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197248.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3135"/>
    <dbReference type="EnsemblBacteria" id="BAD42117">
        <property type="protein sequence ID" value="BAD42117"/>
        <property type="gene ID" value="STH3135"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3135"/>
    <dbReference type="eggNOG" id="COG3880">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102553_1_0_9"/>
    <dbReference type="OMA" id="CGKRPAT"/>
    <dbReference type="OrthoDB" id="1370308at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025542">
        <property type="entry name" value="YacH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38430">
        <property type="entry name" value="PTHR38430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015034">
        <property type="entry name" value="YacH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="134"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="19668" checksum="39501FE9DE1C998C" modified="2004-10-11" version="1">MRCERCGQREATVHQTVITNGQKQESHLCVECAREVGAIPSFSFPNLSMQQLLSSFLGQTLPGAGAGQPQLKAEPTCRHCGMTYSQFAQSGLLGCPRCYQEMEQQLMPLIKRIQGTATHSGKVPKRTGGLARKRRDLKILRAQLASAVQGERYEEAARIRDQIRALEAEIAAGGDNGGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS2</accession>
    <name>Q67JS2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197211.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3096"/>
    <dbReference type="EnsemblBacteria" id="BAD42078">
        <property type="protein sequence ID" value="BAD42078"/>
        <property type="gene ID" value="STH3096"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3096"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_9"/>
    <dbReference type="OMA" id="MEDEQHA"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="23449" checksum="2C7A37A93B613A89" modified="2004-10-11" version="1">MPIRVLIVDDHELLREGLKSLLSREEDMEVVGEAAGGREAVALCRSLRPDVVVLDARLPDVDGVEVCRQLQEEMPEVAVLMLTTFSDSDLVLGAVRAGARGYVVKDVLGMDLKRSIRAVARGEVAMDPKVVGYLMQQMRSPSADKEERIPLNRQQLAIIRLVAQGYTNREIAERMFLSEKTVKGYLAEAMRRMGVKNRVEAAMVASKNGWI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU7</accession>
    <name>RS19_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsS</name>
        <name type="ordered locus">STH3071</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197186.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU7"/>
    <dbReference type="SMR" id="Q67JU7"/>
    <dbReference type="STRING" id="292459.STH3071"/>
    <dbReference type="EnsemblBacteria" id="BAD42053">
        <property type="protein sequence ID" value="BAD42053"/>
        <property type="gene ID" value="STH3071"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3071"/>
    <dbReference type="eggNOG" id="COG0185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_0_1_9"/>
    <dbReference type="OMA" id="FPEMVGH"/>
    <dbReference type="OrthoDB" id="1893906at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129920" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <sequence length="93" mass="10509" checksum="B7F1EB84B1461B0F" modified="2004-10-11" version="1">MGRSLKKGPYIDPSVEKKILAMNEKGEKKVFKTWARDCTIFPEMVGHTIAVHNGKTHVPVYITEEMVGHKLGEFAPTRTFRGHGGDERSSRVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV2</accession>
    <name>RS17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsQ</name>
        <name type="ordered locus">STH3066</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197181.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV2"/>
    <dbReference type="SMR" id="Q67JV2"/>
    <dbReference type="STRING" id="292459.STH3066"/>
    <dbReference type="EnsemblBacteria" id="BAD42048">
        <property type="protein sequence ID" value="BAD42048"/>
        <property type="gene ID" value="STH3066"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3066"/>
    <dbReference type="eggNOG" id="COG0186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_1_0_9"/>
    <dbReference type="OMA" id="HPMYGKF"/>
    <dbReference type="OrthoDB" id="2035880at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000233585" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="94" mass="11001" checksum="47268ACF699FC1F7" modified="2004-10-11" version="1">MTAERNLRKTRVGVVTSDKMDKTVVVAVETLVQHPLYKKRVRRTKKFKAHDEHNQCKVGDKVRIMETRPLSKEKRWRVVEIIESAPDYKSAPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K95</accession>
    <name>Q67K95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2920"/>
    <dbReference type="EnsemblBacteria" id="BAD41903">
        <property type="protein sequence ID" value="BAD41903"/>
        <property type="gene ID" value="STH2920"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2920"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_16_1_9"/>
    <dbReference type="OMA" id="CHEFLQD"/>
    <dbReference type="OrthoDB" id="1878940at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="18393" checksum="C06C42E66259E3A6" modified="2004-10-11" version="1">MTRRHERLTRLPDYGPHNPLQYWYRHVHPLRVVWNFICIYLARYCPSLTLKCWLYRLTGAQIGRNVSIGLAVVFDIFYPHLIRIDDNSVVGYNSVILCHDFRVREAFIGPVHIGRDVLVGANCTILPGVRIGDGAAVSAMSLVNRDIPPGAMAGGVPATVISAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD1</accession>
    <name>Q67KD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197001.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2884"/>
    <dbReference type="EnsemblBacteria" id="BAD41867">
        <property type="protein sequence ID" value="BAD41867"/>
        <property type="gene ID" value="STH2884"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2884"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1712347_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="17772" checksum="207856B734362620" modified="2004-10-11" version="1">MRKRQKAYALIVENGRLLLVRYYYPEENAWYWNFPGGTLEPGETLEEALKRELMEECSVEVEVGPMVLKEAPPGRDYVRYFFRCWITKGRPQVGTDPATNDSIGALEWVDLTDPGRCGGWGRRNLPQVARVLGFQPRRRRRLPPPIPRRAVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD4</accession>
    <name>Q67KD4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argD</name>
        <name evidence="4" type="ordered locus">STH2881</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196998.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2881"/>
    <dbReference type="EnsemblBacteria" id="BAD41864">
        <property type="protein sequence ID" value="BAD41864"/>
        <property type="gene ID" value="STH2881"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2881"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_9"/>
    <dbReference type="OMA" id="PFMVPTY"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="229"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="407" mass="42842" checksum="FCF60A7D30C2167A" modified="2004-10-11" version="1">MADVSGSRSAALMARADAHTMKTYGRYPIALVRGEGVRCWDADGKEYLDFLGGIAVNVLGHAHPALVSAIADQAARLIHCSNLYYSEPMAELAELLTQHGGLDRVFFCNSGAEANEGAIKLARKYQWRKGLKAKNRIVSLTHSFHGRTLATVTATAKPAIQEGFGPLPEGFDYVEAGDVAALRAAVTDRTAAVIVEPVQGEGGVRPLAREFLEAARAICDERGALLIFDEIQCGMGRVGHLFAYQAFGVKPDILTLAKGLGGGVPIGAVCATEEAARGFAPGDHGTTFGGSPLACAAALATVKTLLSEDLPARAAAMGEYLLAGLRRLQAKHPGLVKEVRGMGLMLGIELTRPARPVLEKCHELGLLVNVTADTVVRLLPPYIITAADCDRALEILDQALAAVGSAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE7</accession>
    <name>GRDB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Glycine reductase complex component B subunit gamma</fullName>
            <ecNumber>1.21.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Selenoprotein PB gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">grdB</name>
        <name type="ordered locus">STH2867</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[thioredoxin]-disulfide + acetyl phosphate + H2O + NH4(+) = [thioredoxin]-dithiol + glycine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="EC" id="1.21.4.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha, two beta and two gamma subunits. Component of the glycine reductase complex, together with components A and C. PB is substrate specific.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GrdB/GrdF/GrdH family.</text>
    </comment>
    <dbReference type="EC" id="1.21.4.2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2867"/>
    <dbReference type="PRIDE" id="Q67KE7"/>
    <dbReference type="KEGG" id="sth:STH2867"/>
    <dbReference type="eggNOG" id="COG1978">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053106_0_0_9"/>
    <dbReference type="OMA" id="NPDHIES"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030700">
        <property type="term" value="C:glycine reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030699">
        <property type="term" value="F:glycine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010186">
        <property type="entry name" value="Gly_red_sel_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010187">
        <property type="entry name" value="Various_sel_PB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07355">
        <property type="entry name" value="GRDB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01917">
        <property type="entry name" value="gly_red_sel_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01918">
        <property type="entry name" value="various_sel_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <feature type="chain" id="PRO_0000318649" description="Glycine reductase complex component B subunit gamma">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="429" mass="46050" checksum="0F8E58D9D3D6F92B" modified="2008-02-26" version="2">MRIVHYLNQFFGGIGGEEHAGVRPEVRPGPVGPGMALKAALGDAGEIVATVICGDSWFNENLEEAKATVLALIRQQNPDLVVAGPAFNAGRYGMACGAVAEVVSKELGIPVVTGMFLENPGVEVYRRYAWVVETGNSAASMRTAIPAMAALIRRLAAGQEPEPGTYLERGLRVNTFAAERGSARAVEMLVRKLKGEPYTTEYPMPSFDRVPPNPPVKDLSKAKIALVTSGGIVPKGNPDHIESSSASKYGKYYLGDLDDLTAETHQTAHGGYDPTYANADADRVLPVDVMRELEREGVIGKLHDYWYATVGNGTSVANARAYAREIAEDLKKYEVDAVILTSTUGTCTRCGATMVKEIERAGIPVVHVCTVVPISLTVGANRIVPAVAIPHPLGNPALPPDEERTLRRRLVMKALQALTTPVDGQTVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI1</accession>
    <name>Q67KI1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histidine biosynthesis bifunctional protein HisIE</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="13">Phosphoribosyl-AMP cyclohydrolase</fullName>
                <shortName evidence="13">PRA-CH</shortName>
                <ecNumber evidence="13">3.5.4.19</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="13">Phosphoribosyl-ATP pyrophosphatase</fullName>
                <shortName evidence="13">PRA-PH</shortName>
                <ecNumber evidence="13">3.6.1.31</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">hisI</name>
        <name evidence="13" type="synonym">hisIE</name>
        <name evidence="15" type="ordered locus">STH2832</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 13">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 13">In the C-terminal section; belongs to the PRA-PH family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">In the N-terminal section; belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="13"/>
    <dbReference type="EC" id="3.6.1.31" evidence="13"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2832"/>
    <dbReference type="EnsemblBacteria" id="BAD41817">
        <property type="protein sequence ID" value="BAD41817"/>
        <property type="gene ID" value="STH2832"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2832"/>
    <dbReference type="eggNOG" id="COG0139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0140">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_3_1_9"/>
    <dbReference type="OMA" id="ERSCFHQ"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01019">
        <property type="entry name" value="HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023019">
        <property type="entry name" value="His_synth_HisIE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRA-CH" evidence="14">
        <location>
            <begin position="8"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosyl-AMP cyclohydrolase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoribosyl-ATP pyrophosphohydrolase" evidence="13">
        <location>
            <begin position="113"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD41817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="23974" checksum="3DD57737BCFA8262" modified="2004-10-11" version="1">MRTGQVLMQAYMNEEALRLTLETGYAWYWSRSRQELWQKGGTSGHVQRVREIRTDCDGDSLLLLVEQEGVACHEGTYSCFTRRVDGSPKALIDTAFWPIQPDDTVPYDIGSILRELTAVLAERRAHPDPESYTSRLFRRGPDAYCKKIGEEATEVVLAVKNRDRENLAFEVADLWFHSLVALVDQGLSPADVADQLASRRGRRREQQKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ4</accession>
    <name>Q67KJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196938.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2819"/>
    <dbReference type="EnsemblBacteria" id="BAD41804">
        <property type="protein sequence ID" value="BAD41804"/>
        <property type="gene ID" value="STH2819"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2819"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_0_9"/>
    <dbReference type="OMA" id="GAYKRSK"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="342" mass="37279" checksum="992DF3EC90D86BF2" modified="2004-10-11" version="1">MDLVTGATGFIGSQLVPHLVEQGRQVRILVRSRQKAEAVFGPLCAALEVAEGDLGDEASLARAAAGVDRVYHLASRINFQGSLRRMRAINVEGTRRLLDACAAAGVKRVVHMSSIAAGGPAVKDENGRYRARTEEDEAAPLPDAYGITKLEQERLALSYQERGLEVVVVRPSAVFGPGDPDGMNTLIWMVKNGRLPFYLGSGQAVVNLVFVRDVVRGTVAAMERGRPGEVYHLVGPNLTQEQLFGLLAQVSGGRSPRWAMPVPVLMGAARLATIGARLTFRRRSLVHPHEIRNWTAPWIMSDDKARRELGLVPTDTAAAFRETLQWLQAYKARQAEASRTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL2</accession>
    <name>Q67KL2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2801"/>
    <dbReference type="EnsemblBacteria" id="BAD41786">
        <property type="protein sequence ID" value="BAD41786"/>
        <property type="gene ID" value="STH2801"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2801"/>
    <dbReference type="eggNOG" id="ENOG5030CCA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2169791_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="110" mass="12546" checksum="C1A50A8D9E237113" modified="2004-10-11" version="1">MRLPAQGQPMGVPCREEDDARMQYQTGGMMPLTEKDLSYLKDMMSWELLAAKKAYHYANETQDAECRQAMFQIAEQHQRNLERLLTHLHEHVNQPTTIAVSGADAPTVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL3</accession>
    <name>Q67KL3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2800"/>
    <dbReference type="EnsemblBacteria" id="BAD41785">
        <property type="protein sequence ID" value="BAD41785"/>
        <property type="gene ID" value="STH2800"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2800"/>
    <dbReference type="eggNOG" id="COG5577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1626225_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012851">
        <property type="entry name" value="Spore_coat_CotF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07875">
        <property type="entry name" value="Coat_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="163" mass="18348" checksum="5B1DC1F98A9B571D" modified="2004-10-11" version="1">MRRPSSCEEGRAMQQQGVQPQFSMQGGHLIMAHQEQTGLPKVKDPSMNDRDRMQDLLAQEKYLTSGYNMALIEASHDALFDVIKQNCDASYQMQRQIFNLMFKKGWYKLPVADAQMVQHTFQQFVQYQTQFPFPPGPQVAQMAAQTASGQAGGQGGGMQANRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT7</accession>
    <name>Q67KT7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2724</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196843.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2724"/>
    <dbReference type="EnsemblBacteria" id="BAD41709">
        <property type="protein sequence ID" value="BAD41709"/>
        <property type="gene ID" value="STH2724"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2724"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_9"/>
    <dbReference type="OMA" id="MAISHFT"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="26"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="360"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="393"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="603" mass="65498" checksum="F6ACFE5E7C1260AA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY0</accession>
    <name>Q67KY0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine sulfhydrylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2681</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196803.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2681"/>
    <dbReference type="EnsemblBacteria" id="BAD41666">
        <property type="protein sequence ID" value="BAD41666"/>
        <property type="gene ID" value="STH2681"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2681"/>
    <dbReference type="eggNOG" id="COG2873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_9"/>
    <dbReference type="OMA" id="TTHSQMN"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="435" mass="46329" checksum="9A6D595970EF5F83" modified="2004-10-11" version="1">MPDRSDAASDRYRPATLVVHAGWQPDPLTGAQAVPIYQTTAYAFQSTEHARQLFSLEAEGNIYTRINNPTTAAFEARVAALEGGIGAVAFASGHAALAATVLAICNAGDEIVTSSSLYGGTYNLFATTLPKWGITARFVDAHDLAGFHRAITPRTRLIFGEVIGNPRLDVFDIAGVAAIAHEHGLPLVIDNTFATPMLCRPIEHGADLVIHSATKWLGGHGVAMGGVVVDSGNFDWNSPRFPGFTEPDPSYHGVRYAVDFGRAGFITKVRAQMLRDLGGCQSPFNAFLLLLGLDTLALRMRAISENALEIARYLSEHPAVAWVRYPGLPSHPDHALAQRYLTGGFGGMLVFGLKGGLEAGARFIDSVRLFSHVANVGDARSLVIHPASTTHSQLTPAQREAAGVGDDLIRVSVGFEDVADLIADLEQALEKAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ8</accession>
    <name>Q67KZ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196785.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41648">
        <property type="protein sequence ID" value="BAD41648"/>
        <property type="gene ID" value="STH2663"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2663"/>
    <dbReference type="HOGENOM" id="CLU_1905709_0_0_9"/>
    <dbReference type="OrthoDB" id="2232688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="133" mass="14801" checksum="791136F625FBE273" modified="2004-10-11" version="1">MAWMTILGLDWKGGRFAHPLDIVDVPQKPGVFKLHARRKSGEWEVFYVGQAQNLYSVLLAYLGTIVEGDPQAAGINACAKARIETDEVAYSYAVVTDETERQGCLRSLYEFYRPVCNSPEQIPAVDSVACNPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L00</accession>
    <name>Q67L00_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41646">
        <property type="protein sequence ID" value="BAD41646"/>
        <property type="gene ID" value="STH2661"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2661"/>
    <dbReference type="HOGENOM" id="CLU_2557042_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="9522" checksum="CEB3741BD7C3231E" modified="2004-10-11" version="1">MRRNEILERLRELYHQAHETLERAEHLPERLPLDVQPQSLNYDRDLSRIQGEYNRLVGLAQAEGLLSASDLEAQGLPERMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L56</accession>
    <name>Q67L56_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="4 8">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2605</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196727.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2605"/>
    <dbReference type="EnsemblBacteria" id="BAD41590">
        <property type="protein sequence ID" value="BAD41590"/>
        <property type="gene ID" value="STH2605"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2605"/>
    <dbReference type="eggNOG" id="COG1488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025154_2_1_9"/>
    <dbReference type="OMA" id="VYFPGSP"/>
    <dbReference type="OrthoDB" id="241792at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="PTHR11098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 8" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAPRTase_N" evidence="9">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase_C" evidence="10">
        <location>
            <begin position="362"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="486" mass="54300" checksum="78D840A4A2DE1D47" modified="2004-10-11" version="1">MRSPTPSEGLLADEYALEMMRAYWLAGQHRRRVVKEVFFRKAPFGGRLALAVGINQVARYLAELKLSDEDLEYIAGLGFEPEFVEHLRGLRFTGTMRAVAEGTFVFPGEPIITLVGPIEELTYVEARILNIVNPQTLVATKAFRVVEAAEGDPVIEMGLRRAQGGDASIYHSRAAYIAGVDSTSNLEAGRLFGIPARGTHAHALVMLGGDEKEAFRLWAQSQVKLGRRPTFLIDTYDTLRSGLPNAIAVAREMGIRIGAVRLDSGDLAYLSKECRRILDEAGFRDAKIVASGDLDEYVIRDLKLQKAPIDMWGVGTNMVVAADQPSLGGVYKLTAVEENGAWSPRIKVSENPEKVTHPGGPKQLIRFRSKETGYYLADLLMLPDEPLPDPAQPFLIFDPVHTWKKTLLRNFTVEDLLLTYIQDGRQLLPDQPPDKVTAAAREWVAVQRKRFYEEIQRLTNPAAYKVDLSEPLWKLKMQMLESSTRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE5</accession>
    <name>Q67LE5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196639.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2516"/>
    <dbReference type="EnsemblBacteria" id="BAD41501">
        <property type="protein sequence ID" value="BAD41501"/>
        <property type="gene ID" value="STH2516"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2516"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_3_9"/>
    <dbReference type="OMA" id="CSIWPMI"/>
    <dbReference type="OrthoDB" id="1694312at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="60"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27537" checksum="9893CC9DA6D92E8A" modified="2004-10-11" version="1">MPRYRWYHYAVALAILALAWEGLAIWLNIWRGVAAFPRLGPAVAAFLEGVKPGGPLVEHFLASAYRVIASTLISLALGVPLGLYLGRSGGPVGSVLVYLTYPVPKVVFLPVIMVVLGIGDESKIFLITLVIFFQILVTVKDAAANVPRGLIRSVQSLGGGEAQIYRHVILPAALPAIFTALRLSTGTAVAVLFFAETYATRRGLGSYVWDAWSMHAYSKMFAGVMAMALMGFGFYVVLDIAERYLCPWAEKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI9</accession>
    <name>Q67LI9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196595.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2472"/>
    <dbReference type="EnsemblBacteria" id="BAD41457">
        <property type="protein sequence ID" value="BAD41457"/>
        <property type="gene ID" value="STH2472"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2472"/>
    <dbReference type="eggNOG" id="ENOG502Z9YG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080120_1_0_9"/>
    <dbReference type="OMA" id="ACWRPLF"/>
    <dbReference type="OrthoDB" id="1695614at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046037">
        <property type="entry name" value="DUF5995"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19458">
        <property type="entry name" value="DUF5995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="30086" checksum="4B891DE2BBFC8A85" modified="2004-10-11" version="1">MRMGGAEVNGIDDLAAVMDVHLARFDRDGDHRAAFLRVYRHMTLAVRERLRRPFFLDPAWVERVAVRFGWYYFDALERFERGDDPPPAWAYAFDIAARKRAFLLQDILLGMNAHINNDLPLVVAEILRAEGDERSVFETVRRRFDHDQINRVLHDVIPGVQDEVARHYGRLIRPLGRLMGDLDRQLTTYGLKTWRDQVWRNARCLLAAAGEEERQLVIRFIQQDALHLAREIYRFAPLRLLRPLARWMRRWRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP8</accession>
    <name>Q67LP8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196536.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2413"/>
    <dbReference type="EnsemblBacteria" id="BAD41398">
        <property type="protein sequence ID" value="BAD41398"/>
        <property type="gene ID" value="STH2413"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2413"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1757903_0_0_9"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="16030" checksum="C97D808107D67490" modified="2004-10-11" version="1">MDRTVPWEGFHHIALVTPDLDTTIHFYGNVLGMQVGEVRAGGGVLPQRHCFIRPGEEAATWGLHFFENPSAQIPRVTLEELQHGPFVPGALQHIAFALPDAEAAGAPRARLERHGVQATPTGSIGPIQNMLFIDPNGIVLEATWSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ1</accession>
    <name>Q67LQ1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196533.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2410"/>
    <dbReference type="EnsemblBacteria" id="BAD41395">
        <property type="protein sequence ID" value="BAD41395"/>
        <property type="gene ID" value="STH2410"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2410"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1757903_0_0_9"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="16332" checksum="F627FE5181F255A3" modified="2004-10-11" version="1">MSQPIPWEGFHHIALVTPDLDATIALYGGVLGMQVGEIRVGGGVLPQRHCFIRPGDSVQTWGLHFFENPSAQIHRLTMEQLQQGGFIPGALQHIAFSLPDARAAEALRQRLREHGVEVTPTGTIGPIQNMLFFDPHGILLEATWPRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ5</accession>
    <name>Q67LZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2316</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196439.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2316"/>
    <dbReference type="EnsemblBacteria" id="BAD41301">
        <property type="protein sequence ID" value="BAD41301"/>
        <property type="gene ID" value="STH2316"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2316"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041141_4_1_9"/>
    <dbReference type="OMA" id="AHSGRWY"/>
    <dbReference type="OrthoDB" id="1654295at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028349">
        <property type="entry name" value="PafC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016838">
        <property type="entry name" value="PafC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="3">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="36167" checksum="9321002E3672E0CF" modified="2004-10-11" version="1">MATGSARAAERRFQLLTVLMGGGRPRVDELARRFGVTRRSVFRDLEFLERMGVPIVRDGGRYAVLDTFKVRPIQFQPEEVLALMAALDFGQRKRPVGGTAARSAWEKLLAVLPAAQQELATGLDRTLVVDPIQAYSLPPSPDVEAACRAAVEGPHPLRILYQSLHADAPVERVVRPYGMAYRGTALYLIGFCELRREVRIFRANRILEAQVLSTTFERPPDFDLERFLSDVWGIEFGPLMHVRVRFDREVARLVRETIWHPSQRLEEEEGGAVILRMEARGTGELARWLAGFGGRAEVLEPPELRQAVLELGQGIVRRYGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M27</accession>
    <name>Q67M27_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41268">
        <property type="protein sequence ID" value="BAD41268"/>
        <property type="gene ID" value="STH2283"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2283"/>
    <dbReference type="HOGENOM" id="CLU_2829691_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="66" mass="7427" checksum="2A5DBD39E8402385" modified="2004-10-11" version="1">MQVLHLVRQLLYLVMYALHLVMQVLKPCTYGPVSRRARCPSIREGQCGARQGLGRGTLVWCPAFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M46</accession>
    <name>Q67M46_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6431 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2264"/>
    <dbReference type="EnsemblBacteria" id="BAD41249">
        <property type="protein sequence ID" value="BAD41249"/>
        <property type="gene ID" value="STH2264"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2264"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1320339_0_0_9"/>
    <dbReference type="OMA" id="VHLCMCI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045536">
        <property type="entry name" value="DUF6431"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20020">
        <property type="entry name" value="DUF6431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6431" evidence="1">
        <location>
            <begin position="46"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="221" mass="25494" checksum="8164DC199589D25D" modified="2004-10-11" version="1">MAVHLGRLSPQGGVHLCMCILLPLGQNVQTYLRKYGTCGPDLPLHCPGCGGRMRRHGRYWRWVFTAHQKAYIPIYRWWCPGCRKTCAILPDFLKPYARFITLVREAVVRGRVRRGLPWSTLARRLSSPTVSWLSEKTLRRWLVRARALAGEWSQYLAERVLRFWPDTDLDALTPRREGPDATLHFLLDVGDWYRRQMGRRPEEHGGVFAALNRLGEGTASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M81</accession>
    <name>Q67M81_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2227"/>
    <dbReference type="EnsemblBacteria" id="BAD41212">
        <property type="protein sequence ID" value="BAD41212"/>
        <property type="gene ID" value="STH2227"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2227"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105788_4_1_9"/>
    <dbReference type="OrthoDB" id="1809950at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="19237" checksum="3F57DECB9F04F7AF" modified="2004-10-11" version="1">MPAVLQHKSTITVSWTGRLVTIRPCTPEDAPRVAALYDRLSPRALRLRYCSAVRISGEAEAARLCNSDPADRAVLLALSEGEVIGIGELGRMDEDCAEIALLVRDDCQGEGIGTALGHELVEVARELGYTRLQAYMLAENRGMRRIIAKLPFTRNWEGSWGELCVTVELESPATVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA3</accession>
    <name>Q67MA3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196330.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2205"/>
    <dbReference type="EnsemblBacteria" id="BAD41190">
        <property type="protein sequence ID" value="BAD41190"/>
        <property type="gene ID" value="STH2205"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2205"/>
    <dbReference type="eggNOG" id="COG2068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1824344_0_0_9"/>
    <dbReference type="OrthoDB" id="472474at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="141" mass="14829" checksum="44291F184358FB93" modified="2004-10-11" version="1">MAALHLTEARARDGATAVQTGSAPAETNRGADPAVVLIALGDQPLITPAVVASILTGHRGGLTVPTCAERRGHPFCVDRSLLDDLLTLPPEAGLRELFLRHPGRVTLVPVATDAILRDIDTPSAYRRALAERAGEAQACRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP2</accession>
    <name>Q67MP2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2066</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2066"/>
    <dbReference type="EnsemblBacteria" id="BAD41051">
        <property type="protein sequence ID" value="BAD41051"/>
        <property type="gene ID" value="STH2066"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2066"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_5_9"/>
    <dbReference type="OMA" id="TALFMEN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269275" evidence="8">
        <location>
            <begin position="21"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="8"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="506" mass="53728" checksum="BD067710B26D609C" modified="2004-10-11" version="1">MRDRRMLTVFALMLASFLTAVDVTIVDTAMPRIVGSLGGFSMLTWLVSAYMLTSTATMPVYGKLADIIGRKRTFTIGAVIFVIGSALCGAAPSMEALILFRALQGLGAGAMQPTVQTILGDIFTPAERAKVQGWFSAVWGFSALAGPLVGGLMVDYLSWRYLFYINLPLGALALYMVWRHFDERVQRRKARVDYLGAVTLTGGMTLLLLVLLTGGSQLPWGSPQILGMAGGAAVLLLLFLWTQGRALDPILPLRLFRIPVIGWGNLSSLMVGGVMYGTSVYLPIWAQGVQGYSATRSGASLLWLSIGWPLAAVLGSRLIVRMGVRPSALLGLGLNVAGSAALVALARTMAEIPEVLLALVTFVIGAGMGFSTLAFILGIQSSVDWAQRGVATASFQFIRTLGGMIWVSAMGGVMNLDLLGRLQRIPGVAARTAAEAGQWANRLLDPAQRAALGPELQAAMQEALADALRNVHLVMLLAAVLSLAAALLLPNLRFAPEKAPQASTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR5</accession>
    <name>Q67MR5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2043</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2043"/>
    <dbReference type="EnsemblBacteria" id="BAD41028">
        <property type="protein sequence ID" value="BAD41028"/>
        <property type="gene ID" value="STH2043"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2043"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="ETYRAEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="21"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="116"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="182" mass="20935" checksum="33E602A9F4EE1963" modified="2004-10-11" version="1">MTDRERILSAQRGDREAMASLYNTYRLQAYQTALVVLQDPHLADDVVQEAFIRAFREIGRCDPDRPFAPWIARIVINLSRNALRSRRFLPLPARDRHGTLEPRYAASEARVDLWPVLQRLTRAHREVLLLRYFHGFTEPEIAEILGLPVGTVKSRLHAARQAVRRHLNATDEMSGKEMTPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU3</accession>
    <name>Q67MU3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2015</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196141.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2015"/>
    <dbReference type="EnsemblBacteria" id="BAD41000">
        <property type="protein sequence ID" value="BAD41000"/>
        <property type="gene ID" value="STH2015"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2015"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_4_9"/>
    <dbReference type="OMA" id="FSYLWFM"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="49"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="376"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="409"/>
            <end position="416"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="620" mass="68095" checksum="1CEE55AB3825CA8B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ7</accession>
    <name>Q67MZ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1961"/>
    <dbReference type="EnsemblBacteria" id="BAD40946">
        <property type="protein sequence ID" value="BAD40946"/>
        <property type="gene ID" value="STH1961"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1961"/>
    <dbReference type="eggNOG" id="ENOG50341KI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1354027_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="202" mass="22214" checksum="40790EBCE193446F" modified="2004-10-11" version="1">MNLWTTPVPRRIMEQQPRGGVQPHTEGCGMARRRVLVPALAAGLVLLMGSTALAQPAVRLPQAQDSKFIEDRVEVSVRREDGGLSTQATKYTKTVTITRSFKSLAGLTVAKHYSRTTWTYGGGTLYSSPRGIDVDWWTAPLNNYKTHSAKWDWYTTGKGGTGRSNTQVQFVFGVPTPWGPVGSSYSSRIYTTVNGNGGYSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N01</accession>
    <name>Q67N01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196084.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1957"/>
    <dbReference type="EnsemblBacteria" id="BAD40942">
        <property type="protein sequence ID" value="BAD40942"/>
        <property type="gene ID" value="STH1957"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1957"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1757902_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="16051" checksum="C4EC08B89CB808E0" modified="2004-10-11" version="1">MRVRKLIAETVWEIAGVMLLVLFVVLIATMPPLDLTPNPDSLIGYTIQVDTEQWGQTLRAYLQTLGSGSLGTNRRGHDVAGLLLPRILNTLRLVAISLAFALPLGVVKGLRDFQALRRRGSAVGPLLTGLLQGVPDFFLVMLLPIGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N24</accession>
    <name>Q67N24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1934</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1934"/>
    <dbReference type="EnsemblBacteria" id="BAD40919">
        <property type="protein sequence ID" value="BAD40919"/>
        <property type="gene ID" value="STH1934"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1934"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_3_9"/>
    <dbReference type="OMA" id="SVAMRGW"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="36427" checksum="830E374AFBD871B7" modified="2004-10-11" version="1">MTGRATRGRRAMTLTAVALLAALIVAGALGSVRIPPADVVKILLNRLGADFPATWPRTWEAVLWDVRFPRVAMGALVGMGLSVAGAAYQGLFRNPLADPSVIGASAGASLGAALAIAFFDRVPSLTGLWRLGPVPLFAFAGGVAAVLVAFRLATAGRQTSTVSLLLAGMVVSTVATSLVSLIMYFTDSQARDAIVFWLMGGLSGANWPKAAWLLPYLALGLGLLLWDGRELNAFLLGEEAALSMGVDVERLKRRILAAGSLLTAAAVAFCGIISFVGLIVPHLVRLLVGPDHRWLLPIAAVTGSVVVVAADTVARSLPWVSELPVGLVMSLIGGPVFLVILRRQLLPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N91</accession>
    <name>Q67N91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1599</name>
        <name evidence="3" type="ordered locus">STH1867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">IAM14863</strain>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0236 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195728.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1599"/>
    <dbReference type="EnsemblBacteria" id="BAD40584">
        <property type="protein sequence ID" value="BAD40584"/>
        <property type="gene ID" value="STH1599"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40852">
        <property type="protein sequence ID" value="BAD40852"/>
        <property type="gene ID" value="STH1867"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1599"/>
    <dbReference type="KEGG" id="sth:STH1867"/>
    <dbReference type="eggNOG" id="ENOG5031FFI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040782_3_0_9"/>
    <dbReference type="OMA" id="YRVISHE"/>
    <dbReference type="OrthoDB" id="683481at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009620">
        <property type="entry name" value="UPF0236"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06782">
        <property type="entry name" value="UPF0236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="465" mass="53372" checksum="E7FA13202A21299C" modified="2004-10-11" version="1">MEQIRAAVDFSFQEIERLAWQQTLDCFREALVEALSAIDTALYESRDPSRYVYKEMRSRSVVTKFGPITFQRRYYWDREEERFVFLLDEVLGIAKRQRVSDSVRADAVEASVTAGSYRGAAAELERRDCQVFVSHEAIRQWNLQTGRALAAAEKQQQMTLAGTRRVRVLFIEADGFWPARQRGKKAEVRLFVIHEGWIQRGPGSKEYSLVNRRDFVPEPGRDSWEQLSELLESEYDLSETWVIINGDRALWIREGVTWFPKALYQIDRFHLKRELNHVLRHRPQRLEQAHAALEANDAGRLLAVLDEAHKAETDTDRRGKMRRLLADLRTMPESIRDYRIRLQERGVNVEGLRGVGAAEGAVERYSARLRKVGRSWSESGLMAMLQVMAAYYRGALRGAVQYVERALGLESVNAAAEKVRHRVRETVGRGVDAARHARMPILNAGRNNSGGYSKTFRGFAGLVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK8</accession>
    <name>Q67NK8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1750"/>
    <dbReference type="EnsemblBacteria" id="BAD40735">
        <property type="protein sequence ID" value="BAD40735"/>
        <property type="gene ID" value="STH1750"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1750"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1739600_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="150" mass="16476" checksum="B9E52B01E3B4AE9C" modified="2004-10-11" version="1">MRLRDTRARLILSSPMTRSLQTAAILSRAPDLPLAVEFDLYEWLPDLTQTYDSSAVATAAAAEMSQCGGDWPEGQRLGWEPLSQVRRRVLAVLSRYDEDDGHVIVVCHGTVIPALTGRPLGCGEHIPYRLPPEARPPADSPPRQAPPAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL3</accession>
    <name>Q67NL3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stage V sporulation protein K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spoVK</name>
        <name evidence="4" type="ordered locus">STH1745</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbxX/CfxQ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1745"/>
    <dbReference type="EnsemblBacteria" id="BAD40730">
        <property type="protein sequence ID" value="BAD40730"/>
        <property type="gene ID" value="STH1745"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1745"/>
    <dbReference type="eggNOG" id="COG0464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008749_1_0_9"/>
    <dbReference type="OMA" id="KQALHMM"/>
    <dbReference type="OrthoDB" id="1115436at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041627">
        <property type="entry name" value="AAA_lid_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000641">
        <property type="entry name" value="CbxX/CfxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014232">
        <property type="entry name" value="Spore_V_K"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17866">
        <property type="entry name" value="AAA_lid_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00819">
        <property type="entry name" value="CBXCFQXSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02881">
        <property type="entry name" value="spore_V_K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="101"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="333" mass="37169" checksum="087F8DFE4794B51E" modified="2004-10-11" version="1">MARVRQPERMEEISHWLREGRITPADALQMAIELRRRRTAPRAQPRPAETEQDPDRRVQAVIAELERMVGLGQVKALVRELQAYIAIQLRRAKAGLASEPGSLHMIFTGNPGTGKTTVARLLGRLFKEMGVLPKGHTVEVERADLVGEYIGHTAQRTRDVVRRALGGLLLVDEAYALARGGEKDFGKEAIDHLVRAMEEHRGELVVIFAGYPDEMAWFLRQNPGLRSRIALTLHFPDYTAEELIAIAEQMLEQRDYRLTPDGKAALRQILYGNPWLTAGENGNARAVRNLVERAIRRQALRLVDRSDASRAELMAITRADLESAVAAMAGVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL9</accession>
    <name>Q67NL9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195867.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1739"/>
    <dbReference type="EnsemblBacteria" id="BAD40724">
        <property type="protein sequence ID" value="BAD40724"/>
        <property type="gene ID" value="STH1739"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1739"/>
    <dbReference type="eggNOG" id="ENOG5033DT0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_201614_0_0_9"/>
    <dbReference type="OMA" id="GIFLFID"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="66" mass="7717" checksum="0BCB2C32BE5E126F" modified="2004-10-11" version="1">MNDPRALPWWAWVLVGLILGAQGVLLFLDARRRGARAWFWGLIGLIHFPMPTLLYWLLVVRRGRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ6</accession>
    <name>Q67NQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative dolichyl-phosphate mannose synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195830.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1702"/>
    <dbReference type="EnsemblBacteria" id="BAD40687">
        <property type="protein sequence ID" value="BAD40687"/>
        <property type="gene ID" value="STH1702"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1702"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_2_0_9"/>
    <dbReference type="OMA" id="SDGQHTY"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="372" mass="41323" checksum="3E61A63F6DD2B64B" modified="2004-10-11" version="1">MSSAEATSPYSASSIIIIPALEPPPALVKYVQALLERGFPRIVIVDDGSSPACRSIFDSLARMSGCVVLRHEVNRGKGAALKTALRHCLQQGWQQIYRRVIMVDADGQHAVEDVCGMARASHEHPDAYVLGIRDFNQKSVPARSRFGNRLTSGAFHLFYGRYLRDTQTGLRALPISLWEWATQIAGERFEYETNCLVQAMKQGIPMAEMPIRTLYFNKNENTHYRTLRDSWPIFKILIRHLGAYALAGAIASAVTLAVFAWVDLVWLKGAAPAGRLLAASVVSAAAGSLARLLLHWVLRVRTERNMTDATATYYLLVAVQMLASYLVVLAVHLLTDWTAVWIKLVADILLAVAGYQIHPRWLFKSDHTPEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR9</accession>
    <name>Q67NR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1689"/>
    <dbReference type="EnsemblBacteria" id="BAD40674">
        <property type="protein sequence ID" value="BAD40674"/>
        <property type="gene ID" value="STH1689"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1689"/>
    <dbReference type="eggNOG" id="COG2195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_6_0_9"/>
    <dbReference type="OMA" id="NIVCDRV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010162">
        <property type="entry name" value="PepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42994:SF2">
        <property type="entry name" value="PTHR42994:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01883">
        <property type="entry name" value="PepT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="156"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="36468" checksum="62AD8774AB84D06C" modified="2004-10-11" version="1">MADLLEARLRAMGFSVQRDEAGERIGGETGNLIAKRKGNRGSAPLLLCAHMDRVSPGTGIKPQIKDGVITSDGTTILGADDAAGLAAILEGVQRAIEQGMDMPDLEVVFTIAEEGGLNGSKNLDFSLLSAREAYIIDSSTPVGSIVNQAPAQTKVTYRVIGKAAHGGVEPEKGINALVVAAHALTRMKLGRIDAETTANLGIARGGAATNIVMETFEMQGDVRSLVTEKMEAQVRHMTEIFERTAAEFGARVEADVQFAYGPVNLRPDDRVIQRASAAIRRAGLTPVLKATGGGSDANVFNTRGIPACNLGTGYRGAHSLQESLEVAELERIADVVFALIAEYASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU6</accession>
    <name>Q67NU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195791.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1662"/>
    <dbReference type="EnsemblBacteria" id="BAD40647">
        <property type="protein sequence ID" value="BAD40647"/>
        <property type="gene ID" value="STH1662"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1662"/>
    <dbReference type="HOGENOM" id="CLU_1785916_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="145" mass="16397" checksum="E46B8E9443BB6A54" modified="2004-10-11" version="1">MRQSNGRPLRRKLLGVAPQEAERQLREQAERFALEIQHLREALEQARAEEADLTAQCEALAAEVQEAQRKLERLQKGLERSRTMAPIQALVLAREIADLEDEHAARLAELAAEQERIRAEIAERRASLQQWVTSLLESVAERGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW1</accession>
    <name>Q67NW1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1647</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195776.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1647"/>
    <dbReference type="EnsemblBacteria" id="BAD40632">
        <property type="protein sequence ID" value="BAD40632"/>
        <property type="gene ID" value="STH1647"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1647"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_21_1_9"/>
    <dbReference type="OMA" id="LPQGMPK"/>
    <dbReference type="OrthoDB" id="626183at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005580">
        <property type="entry name" value="DbpA/CsdA_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03880">
        <property type="entry name" value="DbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="10">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="37"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="218"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="526" mass="58458" checksum="0AA0439611869564" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY0</accession>
    <name>Q67NY0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_M56 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1628"/>
    <dbReference type="EnsemblBacteria" id="BAD40613">
        <property type="protein sequence ID" value="BAD40613"/>
        <property type="gene ID" value="STH1628"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1628"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_939849_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008756">
        <property type="entry name" value="Peptidase_M56"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05569">
        <property type="entry name" value="Peptidase_M56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M56" evidence="2">
        <location>
            <begin position="72"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="32415" checksum="592D8662D06E3771" modified="2004-10-11" version="1">MVSLGIWLLVVTLFLVDTEITLRFVGRYADPHLRLGWWTALLLVPWVTLLLSPLRTWAAAGLALAAAWGLVRHAAAYQVLRRRLPGAPEGVVERVRALAREFGVRQLPEILYDPSDRMEPAAVGLFRPALVLTPSALDLPEQDFRAVVAHELAHALRRDPLRLWAGGITGAVLGWHPLIRRACRLFTLEVEMAADWQAAAWAGDRRAYALALGRWGLRRHGDHGSPVGAAFATAPAHLLPRLHYLLDPTAAAPHLRPPLGLRDPGPGRRGSGAARWAHLALAAAYTALSAVMTRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ2</accession>
    <name>Q67NZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1616</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1616"/>
    <dbReference type="EnsemblBacteria" id="BAD40601">
        <property type="protein sequence ID" value="BAD40601"/>
        <property type="gene ID" value="STH1616"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1616"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_2_9"/>
    <dbReference type="OMA" id="VFWYKLQ"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="34785" checksum="B1E8FBFEC1244F4F" modified="2004-10-11" version="1">MAPVRKQAFAPAAAAAVLYLGLAWLRQAGFIGIYWGQIMDLALITTIQAVGLSLIYGFAGQFSLGHAAFYGLGAYVAAFVTRAFGGNTLTFALALAAGAATAGLVALLIGLPILRLRSDYLGIATLGFGIIVKVLLDNADKLSPVLGGARGMAGIPRLTTFHWVFWLAVLSVWLARNLVRSSHGRSCIAVREDEVAADIMGLDTTQFKTLAFVVGCAMAGLAGGLYAHLYSFLHPSSFDFLKSFDVLLVVVLGGLGSLTGTVVTAIGWVFLLEALRGVLGQQFLDWRGVVYALVLIVLMILRPQGLMGGREISLDGVLGRAAARGGAGRGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ8</accession>
    <name>Q67NZ8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tripeptide aminopeptidase</fullName>
            <ecNumber evidence="3">3.4.11.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1610</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of the N-terminal residue from a tripeptide.</text>
            <dbReference type="EC" id="3.4.11.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.4" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195739.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1610"/>
    <dbReference type="EnsemblBacteria" id="BAD40595">
        <property type="protein sequence ID" value="BAD40595"/>
        <property type="gene ID" value="STH1610"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1610"/>
    <dbReference type="eggNOG" id="COG2195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053676_0_0_9"/>
    <dbReference type="OMA" id="WTIDCCE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045148">
        <property type="term" value="F:tripeptide aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010161">
        <property type="entry name" value="Peptidase_M20B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42994:SF1">
        <property type="entry name" value="PTHR42994:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037215">
        <property type="entry name" value="Peptidase_M20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01882">
        <property type="entry name" value="peptidase-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="M20_dimer" evidence="10">
        <location>
            <begin position="215"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037215-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037215-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="416" mass="44821" checksum="C5EB55F52E0542B5" modified="2004-10-11" version="1">MDRPDQAQLLNEGVVAKFLRYVQVDSPSGEGEGVPSTPEQWDMARLLEAELKALGLEEVRVDEHAIVTATLPGNRPGAPAIGLLAHFDTFPGTPGHNVKPLVHRNYDGGEIRLPAGPVIRPEEHPALRRCIGHDIITSDGSTLLGADDKAGVAIIMEIVSRLIRNPDAPRGPIRIGFTPDEETGRGIRLFDVAAFDATAAYTFDGSALGEVEAENFNARNLEVTITGKSAHTGTAKGQMINAVALAAEFCMAIPATMRPESTQGYEGFIHVDAINGSVEEVTMKILLRDFTDEGLAHKQAIVEGLLAGLEQRHPGARTALRQTGGYRNMKAGVARDPRVLDLALEAVRDAGLEPVLKPIRGGTDGAVLTEMGLPCPNLFTGGMNYHSRTEWASAQWMEKAVEVGLNLVRRWAEERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P23</accession>
    <name>Q67P23_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">STH1585</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195714.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1585"/>
    <dbReference type="MEROPS" id="S11.004"/>
    <dbReference type="EnsemblBacteria" id="BAD40570">
        <property type="protein sequence ID" value="BAD40570"/>
        <property type="gene ID" value="STH1585"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1585"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_7_3_9"/>
    <dbReference type="OMA" id="IQAGSWV"/>
    <dbReference type="OrthoDB" id="1499212at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269294" description="Serine-type D-Ala-D-Ala carboxypeptidase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="PBP5_C" evidence="16">
        <location>
            <begin position="283"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="12">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD40570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="401" mass="43138" checksum="4F89B97223AAAE3F" modified="2004-10-11" version="1">MNCVRRAIAGMIAALLLISGAEAAAAPRPLVDHYRDVVESPPSLWAHGAILMDAATGDVLWERNAHVRLHPASTTKVLTALIALERGDLDARVTVSKRAAQTPGSSMYLREGEVHTLHDLVHGLLMLSGNDAAVAIAEHIAGSVEAFADLMNERAERLGARNSHFVNPHGLTHPEHLSTAYDLAVITRAALQNPLFAEIVAAPQKELTYEELGRKVVLYNTNRLLRGGLPGADGVKTGTTGAAGPCLIASATRDDQKLIAVVLNASNRWRESAALLEWGFRNFALARLGRAGEVLVHLPVRGGKRRAVPVALREELAVVVPRKTGAVPEVQVDLKRPLRAPLRAGEVVGRAWVPLPGDGRRRVDLIAAEPLPAATWVDRVYQGLVALVDLEGQLNLNLITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P46</accession>
    <name>Q67P46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195691.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40547">
        <property type="protein sequence ID" value="BAD40547"/>
        <property type="gene ID" value="STH1562"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1562"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="OrthoDB" id="1529829at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="340" mass="39051" checksum="129AF5EE387401E4" modified="2004-10-11" version="1">MFSPGISVPDALIRTRHLSEGAKMFWCYLRTVRKEPVSFKQLRARTGLAQNSILKYLRELSRTGWLEYRRGRYSLTCHAIWKDGRPAFRLPVDLICDRGLPAAAKLVWGAITQLKDGFSYAELVRCTRYSRNTVAKYVRLLLQKGWLRGKAHREARRKRFLVQPANPHHERRQADLNLFQRAKKLAEKRIGDSIGQLYATYMVSLIVREDIIICNAQPWGLVNPRTGARLQYDILLPRSRVAIEFQGPQHDGPTALYPDEAQFRAQQERDHLKRQRSAELGIRLIEVRAQDLTFPRLAQLLQEAGVPVTSQPCAAPHLYELLEKETAAYRKAVSEVTRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA0</accession>
    <name>Q67PA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1508</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1508"/>
    <dbReference type="EnsemblBacteria" id="BAD40493">
        <property type="protein sequence ID" value="BAD40493"/>
        <property type="gene ID" value="STH1508"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1508"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_0_0_9"/>
    <dbReference type="OMA" id="LCIFWRA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="30532" checksum="09550AAB7F06D365" modified="2004-10-11" version="1">MLGLAGGVLGAFALLRRRALLGDVLAHAALPGIALAYMATGTKAAGPLLIGATASGVLGVLAMRAITRWSRLKEDAAMSLVLTVFFGLGIMLLGWVQRMPGGNQSGLDKFLFGQAASIVPRDLQVIAVAAALLCLAILVLYKEFKLLAFDPDFGAVLGFPVVALDLLLSLCIALAVVIGLEAVGVVLMAALLTTPAVAARYWTHRLSVMVPLAGLFGAASGVVGTLASQIGPRMPTGPLIVLAASLLFFLSLLAAPHRGLLARLIRLRRAQAQASPRRPDGTPLGQAAAGPRPGDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB0</accession>
    <name>Q67PB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1498</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1498"/>
    <dbReference type="EnsemblBacteria" id="BAD40483">
        <property type="protein sequence ID" value="BAD40483"/>
        <property type="gene ID" value="STH1498"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1498"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_4_0_9"/>
    <dbReference type="OMA" id="CIFIGLE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014227">
        <property type="entry name" value="YtvI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02872">
        <property type="entry name" value="spore_ytvI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="38993" checksum="532FA68B8AC1E5C4" modified="2004-10-11" version="1">MGVLTAMDRAWLYSVLGLAGFLVVSWALITYALPIVMPFVLAWVLAEMMDPAVTWLVRRARMPRSLAVAVVLLFFVGIMVALFTVGVAYVVGEIRALIRNLPYLYAAGLDISSQVLLYLEDLVGSLPESVQVTIGENLGKLQANLGTYLETLARRLGFITSVPGFLINLIITFVAAFFITRDRQEIGAFLLSLFPAKWRDQLRQVRVEVLTNAIGWAKAQVLLILMTMLVSMLGLAIIGADYVVLAGLAIGLLDILPIVGPGALYAPWILYSAFTGRLAFGVKLLIVYAVVSALRQVLESKVVGDRVGLHPLAVLLSIYLGIKFFGALGVVFGPLITILLKAMVTSGLLPIFTGDGRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB8</accession>
    <name>Q67PB8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1490"/>
    <dbReference type="EnsemblBacteria" id="BAD40475">
        <property type="protein sequence ID" value="BAD40475"/>
        <property type="gene ID" value="STH1490"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1490"/>
    <dbReference type="HOGENOM" id="CLU_2332604_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="10946" checksum="6DE6F5F13EE7E87F" modified="2004-10-11" version="1">MNLLRPRAVSTVETADQGRGPRVARWLTERELLEQAGISPAELRWFGDRFDAQMAVLTRRGPDGRPRYAPDAVVLLRSLAAMVARGATPEQIKAWYGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF5</accession>
    <name>RNC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease 3</fullName>
            <ecNumber evidence="1">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribonuclease III</fullName>
            <shortName evidence="1">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnc</name>
        <name type="ordered locus">STH1453</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195583.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PF5"/>
    <dbReference type="SMR" id="Q67PF5"/>
    <dbReference type="STRING" id="292459.STH1453"/>
    <dbReference type="EnsemblBacteria" id="BAD40438">
        <property type="protein sequence ID" value="BAD40438"/>
        <property type="gene ID" value="STH1453"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1453"/>
    <dbReference type="eggNOG" id="COG0571">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_1_3_9"/>
    <dbReference type="OMA" id="LTHKSCK"/>
    <dbReference type="OrthoDB" id="1890943at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0507">mRNA processing</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000228595" description="Ribonuclease 3">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="11"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="1">
        <location>
            <begin position="164"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <sequence length="235" mass="26180" checksum="120B4E1E32097E5D" modified="2004-10-11" version="1">MAVSVLDPGRRAWCAEALGYEFRDESLLLEALTHTTYANEHPRARANERLEFLGDSVLGMVIAAHLYERYPDLPEGELTKIRAAVVCEPSLAERARVLGIGRHMRFGRGEAVSGRDRDSTLSDAFEAVVGALYLDGGLEAAQRFVLRELGQLVEAARQGLVRVDYKTQLQEQLQRQGAAAPQYRLLVEEGPAHLRRFQVGVYFEGRLLGTGWGRNKKEAEQEAARQALMPEHHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM3</accession>
    <name>Q67PM3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1385"/>
    <dbReference type="EnsemblBacteria" id="BAD40370">
        <property type="protein sequence ID" value="BAD40370"/>
        <property type="gene ID" value="STH1385"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1385"/>
    <dbReference type="HOGENOM" id="CLU_729416_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269600" evidence="2">
        <location>
            <begin position="30"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="379" mass="40352" checksum="02E0A6A49AA3920E" modified="2004-10-11" version="1">MVRLTRCRRRTLLLLAALALALLPAGALANAGPPRRAGDTGGPLLPGTSDQVHVLGEELRFDLGPDLGSATVTARYRLANRGPDLEDQRFVFVVQDVGGRTDLAVSWNGEPVPVRLGMDQLGPEELAEMARAWTSVDQWLDPVTGEPYEAEFYGSDPVLRYYLFSLDMPAGAEGELAVTYDHTAGSDRTRYTYRVHHYSYLLLPARGWASFGPLQIRVAAPAGSRYYFAANLDFRDEGGEYVAEYPGLPEANLTFAVMNRAGLFLGPQPGPYYGLGFALVLVLSAAVGVGIGRLAGRLPRPALATGVGVLAALVPTGPALVWLSVLLLSSGLPQLRDQAYASAFAGFATGLVGAALCAAVAGWTARGTWRRRRPAGGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN2</accession>
    <name>Q67PN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195506.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1376"/>
    <dbReference type="EnsemblBacteria" id="BAD40361">
        <property type="protein sequence ID" value="BAD40361"/>
        <property type="gene ID" value="STH1376"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1376"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041132_0_0_9"/>
    <dbReference type="OMA" id="YYCGDDF"/>
    <dbReference type="OrthoDB" id="630753at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="37055" checksum="B1C1ED10D5CFAA76" modified="2004-10-11" version="1">MTCSVIYPPTLDWPWLIQRPQQLMRQFARHGYPTYFCNLTQTPGRAPEPVAPNLWVVHDHEAFVAGGLPGGARLVWCSWPKLHDTLDRYRPTAVVFDAVDDFPEWRPYEGPMLRRADAVVTTARSLFERLSGLHSRVVLVPNGWDKDLARGPLLPRPADLPPSPVAGFVGAWAPWVDGRLLEEVAARLPRWQFVIIGPLHGRRPARGRNIHHLGHRAHGELGRYLRHLDVGLIPFQITPVTRAANPVKLWEYLGVGLPVVSTPLPEVLPLGEHVRFASDPDGFARAIEAAWQTDTPERRQARRQVAAQNSWVARFARIQAELADLLDGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ4</accession>
    <name>Q67PQ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit biogenesis GTPase RsgA</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rsgA</name>
        <name evidence="10" type="ordered locus">STH1354</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Associates with 30S ribosomal subunit, binds 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195484.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1354"/>
    <dbReference type="EnsemblBacteria" id="BAD40339">
        <property type="protein sequence ID" value="BAD40339"/>
        <property type="gene ID" value="STH1354"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1354"/>
    <dbReference type="eggNOG" id="COG1162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033617_2_1_9"/>
    <dbReference type="OMA" id="CLVAAYD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04466">
        <property type="entry name" value="S1_YloQ_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01854">
        <property type="entry name" value="YjeQ_EngC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01820">
        <property type="entry name" value="GTPase_RsgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004881">
        <property type="entry name" value="Ribosome_biogen_GTPase_RsgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010914">
        <property type="entry name" value="RsgA_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031944">
        <property type="entry name" value="RsgA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32120">
        <property type="entry name" value="PTHR32120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03193">
        <property type="entry name" value="RsgA_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16745">
        <property type="entry name" value="RsgA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00157">
        <property type="entry name" value="TIGR00157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50936">
        <property type="entry name" value="ENGC_GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="63"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="ENGC_GTPASE" evidence="8">
        <location>
            <begin position="72"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="292" mass="32981" checksum="DB879CFF13714EA8" modified="2004-10-11" version="1">MPVGQVIRSHSNIYYVLVDGREVECRPRGKFRLDRLQVRAGDEVEVTLAPDGEGRIEKVLPRRNCLERPPVANVDQCLVVFTLVEPEADYLFLDRVLVHVEQAGVEPLILLNKIDLVEEADVAAFVRLYGDLVGYRVLPISAAEGRGLDELRRHLAGKTSVLAGHSGVGKSRLVRALEPQRADVRIGDLSAKSRKGRHTTRHVELIPLTGGGLLADAPGFTYLEFQDIDKWTLRDCFREFARYQAECRYADCLHRKEPDCAVRDAVEAGAIPESRHRNYLMFLDEVEAMKRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ7</accession>
    <name>RLMN_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="1">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmN</name>
        <name type="ordered locus">STH1351</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PQ7"/>
    <dbReference type="SMR" id="Q67PQ7"/>
    <dbReference type="STRING" id="292459.STH1351"/>
    <dbReference type="EnsemblBacteria" id="BAD40336">
        <property type="protein sequence ID" value="BAD40336"/>
        <property type="gene ID" value="STH1351"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1351"/>
    <dbReference type="eggNOG" id="COG0820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029101_0_1_9"/>
    <dbReference type="OMA" id="QVGCSLD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000350478" description="Probable dual-specificity RNA methyltransferase RlmN">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="119"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="1">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="182"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="(transient)" evidence="1">
        <location>
            <begin position="126"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="371" mass="41503" checksum="7B1810DE396F7D51" modified="2004-10-11" version="1">MQESGYVPLKIADPALLFAELGRQPLPGMSLEEMADLMADLGEPRFRAKQLFQWVYQKGVKDFDAMTNLPARLRQHLAGTTMLRLLEKETEQHDRRTGTTKYLFRLADGSQVESVLMRQSWGNSVCVTTQVGCRMGCTFCASTVGGLVRNLTAGEIVDQIVMMQRELPQGERISTVVLMGSGEPLENYDHVLKAVRLVHDPEGLNIGYRHITISTSGIVPGMRRLAEEGLPITLALSLHAPTDELRRQLMPVARIWPLAEVLAAAREYGEKTGRRVTYEYILIEGVNDGPEEARQLARLLKGALAHVNLIPMNPVAERPQYRRPGPERVNRFKEILESNGIATTVRREMGGEIDAACGQLRNRAQRKHMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR1</accession>
    <name>Q67PR1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195477.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40332">
        <property type="protein sequence ID" value="BAD40332"/>
        <property type="gene ID" value="STH1347"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1347"/>
    <dbReference type="HOGENOM" id="CLU_2940148_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="60" mass="6848" checksum="D930C0C3B6E1FCA1" modified="2004-10-11" version="1">MRRCLAYLLGHLIVAAEYLDTDVLQQYVAWSEELGVTLPEVPYRPLPGYPPYPQVEAREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT4</accession>
    <name>Q67PT4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1324"/>
    <dbReference type="EnsemblBacteria" id="BAD40309">
        <property type="protein sequence ID" value="BAD40309"/>
        <property type="gene ID" value="STH1324"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1324"/>
    <dbReference type="HOGENOM" id="CLU_3030776_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="55" mass="5961" checksum="77E7C4CCFB57A62C" modified="2004-10-11" version="1">MSGHDGRDRTMENVRSLQDLGRPVEEARKARTARSATSTATEATARPLTYNGRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW6</accession>
    <name>Q67PW6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1292"/>
    <dbReference type="EnsemblBacteria" id="BAD40277">
        <property type="protein sequence ID" value="BAD40277"/>
        <property type="gene ID" value="STH1292"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1292"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="296"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="552" mass="62225" checksum="CA00167830C3624A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY5</accession>
    <name>Q67PY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Xylulose kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195404.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1273"/>
    <dbReference type="EnsemblBacteria" id="BAD40258">
        <property type="protein sequence ID" value="BAD40258"/>
        <property type="gene ID" value="STH1273"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1273"/>
    <dbReference type="eggNOG" id="COG1070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_3_3_9"/>
    <dbReference type="OMA" id="WLQIIAD"/>
    <dbReference type="OrthoDB" id="1619686at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="2">
        <location>
            <begin position="5"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="3">
        <location>
            <begin position="292"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="539" mass="58485" checksum="7C5A12CF67AFCE7A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q26</accession>
    <name>Q67Q26_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tatA</name>
        <name evidence="8" type="ordered locus">STH1232</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms a complex with TatC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TatA/E family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195363.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1232"/>
    <dbReference type="EnsemblBacteria" id="BAD40217">
        <property type="protein sequence ID" value="BAD40217"/>
        <property type="gene ID" value="STH1232"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1232"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_6_0_9"/>
    <dbReference type="OMA" id="LGMGMPE"/>
    <dbReference type="OrthoDB" id="1907955at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="67" mass="7238" checksum="AB4CDB5E4CC199C1" modified="2004-10-11" version="1">MGRLGTTEIILILVVALLIFGPKKLPELARSVGESIRGFKDAMKGGLGEDIAEVKKELQEVKDSVTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q92</accession>
    <name>TGT_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Queuine tRNA-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgt</name>
        <name type="ordered locus">STH1166</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanine + guanosine(34) in tRNA = 7-aminomethyl-7-carbaguanosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:24104"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:58703"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="EC" id="2.4.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Within each dimer, one monomer is responsible for RNA recognition and catalysis, while the other monomer binds to the replacement base PreQ1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.29" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195297.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q92"/>
    <dbReference type="SMR" id="Q67Q92"/>
    <dbReference type="STRING" id="292459.STH1166"/>
    <dbReference type="EnsemblBacteria" id="BAD40151">
        <property type="protein sequence ID" value="BAD40151"/>
        <property type="gene ID" value="STH1166"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1166"/>
    <dbReference type="eggNOG" id="COG0343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022060_0_1_9"/>
    <dbReference type="OMA" id="GIDLFDC"/>
    <dbReference type="OrthoDB" id="1165356at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:queuine tRNA-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101030">
        <property type="term" value="P:tRNA-guanine transglycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000016879" description="Queuine tRNA-ribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="1">
        <location>
            <begin position="248"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="1">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="400" mass="44730" checksum="4FE0123F5D4A5668" modified="2004-10-11" version="1">MAVRWELLRRDPASAARLGRLHTPRGVIETPVFMPVGTQATVKTLNPEEVWDLGARIILSNTYHLYLRPGHDLVQEAGGLHRFMNWKGAVLTDSGGFQVFSLADLRKITEEGVQFRSHIDGSTHFLSPEKAIAVENALGADIIMAFDECTPWPCDYDYAKRSIERTARWAARCKAAHARPDEQALFGIVQGSTFADLRRQSAEEIVALDFPGYGIGGLSVGEPKELMHEMLEVQVPLLPDDRPRYLMGVGSPEDLVEGVWRGVDMFDCVLPTRIARHGTVFVPDGKMTVRNAEFARDFLPIQEGCDCYACRNFSRAYIRHLLKADEMLGLRLCSIHNLRFLVRLMEEIRAALAAGTFAEYRKAFLERWHAGEAERRERARAAGGAGHAPGPAEPLLPENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC2</accession>
    <name>Q67QC2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40121">
        <property type="protein sequence ID" value="BAD40121"/>
        <property type="gene ID" value="STH1136"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1136"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="289"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="552" mass="62216" checksum="C3CE3777F254BD12" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI1</accession>
    <name>Q67QI1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195209.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1077"/>
    <dbReference type="EnsemblBacteria" id="BAD40062">
        <property type="protein sequence ID" value="BAD40062"/>
        <property type="gene ID" value="STH1077"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1077"/>
    <dbReference type="HOGENOM" id="CLU_834007_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="333" mass="36559" checksum="105156AEC59D246D" modified="2004-10-11" version="1">MRIRTIATGLLLAAALVGCSLGPDRPLTELPEGAEFAPAGAVSGFAIRGELHLYPHHELVGGGLASFIVHVESLSDEGVRGLRMAVFYPEEIALVSDVEPYLESPRGYDLTPDQPSYGLGRTFTFPDWGQAHVVRQLAEKPLRVHLVWDGGEQFLEVPPEAIRVTQSDEPRRLANDPDLFSFPTHPAFLDHPPFSEGRYLSRARDVTAADLLAWYRAEMPASGWEALPAPDRALLFRKGEMFLSLAAADEPDGTTVMWSHLRGTAEVPEDAAVRIVRARYPESRENQWVATYLADGAGSGADSPVWEVRGLRDGKVWVTAWVDAVTAELRVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS3</accession>
    <name>Q67QS3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195117.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH985"/>
    <dbReference type="EnsemblBacteria" id="BAD39970">
        <property type="protein sequence ID" value="BAD39970"/>
        <property type="gene ID" value="STH985"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH985"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_2_9"/>
    <dbReference type="OMA" id="WDLRAVW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="31"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="331" mass="36602" checksum="A7943DD9C2097070" modified="2004-10-11" version="1">MRAEPPPYQPPAEVSPGIFLVPIPIPIPLRYVNCYLLRGPSGWTLVDTGFHDSLAEAAWPRALAHLAIRAQDIKQIVLTHYHPDHVGAAGWLQQLTGAPAFLPETEMEQFHRFWADGTGAMAERLQALFLGEGMDEATASALRRHHLQQVERVRPLPELRPLAIDSRIAMGDGTYEVIWTPGHSDGLAVFWDARRSILLANDMLLSGITPNVSVWPGSRPNPLADYLASLRRVAALGARLALPGHRALIRDVAGRAREILAHHAERLARVTDAAAASPGGATAWEICRRIFPVDGLTLHQVRFAMAETLAHLVYLCREGRLRKEGMRYLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT3</accession>
    <name>Q67QT3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH975"/>
    <dbReference type="EnsemblBacteria" id="BAD39960">
        <property type="protein sequence ID" value="BAD39960"/>
        <property type="gene ID" value="STH975"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH975"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514738_0_0_9"/>
    <dbReference type="OMA" id="QQGFNIM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269334" evidence="3">
        <location>
            <begin position="28"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="4">
        <location>
            <begin position="73"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="5">
        <location>
            <begin position="243"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="103"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="204"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="517" mass="54621" checksum="8CC35B51CBCBECBA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT4</accession>
    <name>Q67QT4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Yip1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH974</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195106.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH974"/>
    <dbReference type="EnsemblBacteria" id="BAD39959">
        <property type="protein sequence ID" value="BAD39959"/>
        <property type="gene ID" value="STH974"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH974"/>
    <dbReference type="HOGENOM" id="CLU_1146721_0_0_9"/>
    <dbReference type="OMA" id="RSASWWL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="6">
        <location>
            <begin position="22"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="25351" checksum="7A225E9B8265C543" modified="2004-10-11" version="1">MSEPVLNHGDGRRVSPWAAVLRVLTDPAETFRAMAAHPPVLVPYLVHTLVGLAVFALTYGPTVDLAMEAAVASLPPGQEVDFQMMQSVMRWSAVGTLAAGSVIGPWLTGFVLALVATFFGQFQGGGVPLASYMGMIGYARLPVAISNLLAGILMAVTGRPVNLTPAVLLPDTAGPVLAAVLSAFNPLHLWYYVLLGIGFAALFGREPRKGWALPLALFVMTTLFTAASAGLSSALTSSMTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT9</accession>
    <name>Q67QT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">S-layer associated protein, putataive</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH969"/>
    <dbReference type="EnsemblBacteria" id="BAD39954">
        <property type="protein sequence ID" value="BAD39954"/>
        <property type="gene ID" value="STH969"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH969"/>
    <dbReference type="eggNOG" id="COG3210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512781_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="57"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="181"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="531" mass="55611" checksum="83F571A44F6A8098" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R60</accession>
    <name>Q67R60_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA polymerase I</fullName>
            <ecNumber evidence="7">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">polA</name>
        <name evidence="10" type="ordered locus">STH848</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Single-chain monomer with multiple functions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194980.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH848"/>
    <dbReference type="EnsemblBacteria" id="BAD39833">
        <property type="protein sequence ID" value="BAD39833"/>
        <property type="gene ID" value="STH848"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH848"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_0_0_9"/>
    <dbReference type="OMA" id="NRPPMPD"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="8">
        <location>
            <begin position="3"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="9">
        <location>
            <begin position="624"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="874" mass="96744" checksum="B5D7DFCCD2225EAC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE7</accession>
    <name>PSTB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB 2</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB2</name>
        <name type="ordered locus">STH761</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194894.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RE7"/>
    <dbReference type="SMR" id="Q67RE7"/>
    <dbReference type="STRING" id="292459.STH761"/>
    <dbReference type="EnsemblBacteria" id="BAD39746">
        <property type="protein sequence ID" value="BAD39746"/>
        <property type="gene ID" value="STH761"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH761"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="TIDICRV"/>
    <dbReference type="OrthoDB" id="1416748at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092912" description="Phosphate import ATP-binding protein PstB 2">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="18"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <sequence length="262" mass="28903" checksum="CAC5DA51322428AD" modified="2004-10-11" version="1">MAVVLASPAAGGVAAGGFVVRNLDLFYGTHQALHQISMTIPERAVTAIIGPSGCGKSTFLRCLNRMNDLVPDARIQGHVSFRGQDLYAPGADPVEIRYRIGMIFQKPNPFPKSIYENVAFGPRINGYDGDLDEIVEGALRRAALWDEVKDRLRKPALTLSGGQQQRLCIARALAVNPEVLLMDEPTSALDPIATGKIEELMTSLKQEYTIVLVTHSMQQAARVSDYTAFFLDGRLVEMDRTERIFSTPSDRRTEDYITGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF3</accession>
    <name>Q67RF3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Minor extracellular serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH755"/>
    <dbReference type="MEROPS" id="S08.020"/>
    <dbReference type="EnsemblBacteria" id="BAD39740">
        <property type="protein sequence ID" value="BAD39740"/>
        <property type="gene ID" value="STH755"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH755"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005356_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07474">
        <property type="entry name" value="Peptidases_S8_subtilisin_Vpr-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041286">
        <property type="entry name" value="MBG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034213">
        <property type="entry name" value="S8_Vpr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18676">
        <property type="entry name" value="MBG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="8">
        <location>
            <begin position="37"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="126"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="7">
        <location>
            <begin position="390"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="MBG_2" evidence="9">
        <location>
            <begin position="1131"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1313" mass="139344" checksum="C8103F6AC11D333B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF8</accession>
    <name>Q67RF8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH750</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH750"/>
    <dbReference type="EnsemblBacteria" id="BAD39735">
        <property type="protein sequence ID" value="BAD39735"/>
        <property type="gene ID" value="STH750"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH750"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="SYPRRVW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="7"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="26698" checksum="63E41FA1E4575509" modified="2004-10-11" version="1">MFPVPERILVVDDEPSILELVAYNLRRAGFEVLTADNGEDGLRIAREEEIDLVILDVMLPGIDGFEVLRALRRHSELPVLMLTARGEEIDRVVGFEIGADDYVTKPFSPRELTGRVKAILRRARRSAEPQDQDTLVLGPLTINFASYEVLRDGRRVDLTPTEFQILGVLARSPGRVFTRDELVDRVMGPDFYGDVRTVDVHIRHLRAKLEENPSEPRLIETVRGVGYRFAGPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH6</accession>
    <name>Q67RH6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH732</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="BAD39717"/>
        <property type="gene ID" value="STH732"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH732"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_64_9"/>
    <dbReference type="OMA" id="RYVIVKP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021796">
        <property type="entry name" value="DUF3365"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11845">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
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        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="272"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="361"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="584" mass="64285" checksum="17CC4524F336A07F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ0</accession>
    <name>Q67RJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bacillopeptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194851.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH718"/>
    <dbReference type="EnsemblBacteria" id="BAD39703">
        <property type="protein sequence ID" value="BAD39703"/>
        <property type="gene ID" value="STH718"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH718"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002614_0_0_9"/>
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    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033857">
        <property type="entry name" value="Bacillopeptidase_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR008757">
        <property type="entry name" value="Peptidase_M6-like_domain"/>
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    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
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    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
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    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
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        <property type="entry name" value="PKD/Chitinase_dom"/>
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        <property type="entry name" value="PKD_dom"/>
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    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
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        <property type="entry name" value="Peptidase_M6"/>
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        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SUBTILISIN"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269951" evidence="5">
        <location>
            <begin position="29"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="7">
        <location>
            <begin position="799"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="6">
        <location>
            <begin position="1727"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="6">
        <location>
            <begin position="1815"/>
            <end position="1899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1707"/>
            <end position="1728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1899" mass="200843" checksum="C129094EC3491B38" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN8</accession>
    <name>Q67RN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39655">
        <property type="protein sequence ID" value="BAD39655"/>
        <property type="gene ID" value="STH670"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH670"/>
    <dbReference type="HOGENOM" id="CLU_021293_3_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="6">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="5">
        <location>
            <begin position="258"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="342" mass="38771" checksum="BD9DEFFE0E0ADEE3" modified="2004-10-11" version="1">MVNAVLREHGHAQRKYELVLIEDLVPQDHLLRKIQAILDTDFIRERTARFYSDRGRPAIDPVVLVKMELIAYLFGIRSDRRLVEEIRVNVAYRWFLGLGLTDPVPHFTTPGKNYSRRWKDSGLFEELFDHVVKQAIDAGYIDGRMIFTDSSHLKANANKRRIAKEGTKGVTLEDIARARERHLAARRAEREECAANDDTEDGLLAAVNADREAHGLKPLPERKEDPQPDLSVDEMTVSLTDPEAAMLRREGKPDGFHYLQHRTVDGRHGFILDVLVTSAAMTDAQVYPTCLSRVDRHGLKVEKVGVDAGYNTLEVLHLLSKRGIQAAVAHRRHPSPKELMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ7</accession>
    <name>Q67RQ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH651"/>
    <dbReference type="EnsemblBacteria" id="BAD39636">
        <property type="protein sequence ID" value="BAD39636"/>
        <property type="gene ID" value="STH651"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH651"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_13_3_9"/>
    <dbReference type="OMA" id="VFPWDRY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269512" evidence="2">
        <location>
            <begin position="27"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="72"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="364" mass="39262" checksum="0F78E0A5FD6F3B82" modified="2004-10-11" version="1">MVLVPLLSACGGAQSAGPSGQTPQPAAPSAEPQQQEETSAAPEPAQEPAQPTPRTFVDQAGRTVTLPAEVNRVVTTWRPGTLLVISAGGRDKIVGVDSSSQKSEFLTAVFPGILDLPGVGNKKGLNVEQIVAVKPDVVFIWSGSDTEPVIQQLETQGIPAFVLIPESLDQMKEAVSLIGEILGTREQAQKMIQYYDDTLAMLKEKVGAIPEAEKLRVYMVGSSGVFSTVPGTMYQHFIIESAGGINVGAELEGGFSEVSAEQVVAWNPDVIVAVQYCDCTVEDILSNPQLQTVKAVRDGRVYMFPSSMDPWDYPEPRSILGMLWLATKLYPERMADVNLLEEVDTFHETFFGKSFTELGGTLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS4</accession>
    <name>Q67RS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194767.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH634"/>
    <dbReference type="EnsemblBacteria" id="BAD39619">
        <property type="protein sequence ID" value="BAD39619"/>
        <property type="gene ID" value="STH634"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH634"/>
    <dbReference type="eggNOG" id="COG0400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076826_0_0_9"/>
    <dbReference type="OMA" id="WNPHTLM"/>
    <dbReference type="OrthoDB" id="523836at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="34163" checksum="67BB6EF30E9B5E3A" modified="2004-10-11" version="1">MAIRDFSDLQREVFRLYNQGAYREALALIDREAGRFPDYAPTFYFWRACLACRADGADAGLRWLQAAAEEGLWYHERMLNDPDLAPLRGDPRFEPLLKLFRERWEAAQAVARPVLRTYEPEGEPQGLLVALHGASSGFEQEEERARWTAATAAGWRVALAQSSQVWGPGLYSWRDRDRALAEVRAHLDSLGPHDPTVMAGFSMGAAVAMFGVLTGAFPATRFLAVAPAIRLETFRPLLAEARRDVRGYILIGDGDWMFKEVQEFASAMRDAGLACELEVHPGLGHDFPTGFAADLPRRLAVLNSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S58</accession>
    <name>Q67S58_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="1 3">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1 3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194634.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH500"/>
    <dbReference type="EnsemblBacteria" id="BAD39485">
        <property type="protein sequence ID" value="BAD39485"/>
        <property type="gene ID" value="STH500"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH500"/>
    <dbReference type="eggNOG" id="COG0634">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073615_0_0_9"/>
    <dbReference type="OMA" id="TMDWMAV"/>
    <dbReference type="OrthoDB" id="1819460at2"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="4">
        <location>
            <begin position="14"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="20244" checksum="E8BE0AF9180F17A4" modified="2004-10-11" version="1">MQQDIERVLITEEQIREKIKELGAQISEDFRGKDLVVVGILKGAALFCADLFRAISIPAELDFMRITSYGAATKSTGVHRVLLDLDYTLEGRHVLIVEDIVDTGLTIKFLKEYLSQRGPASLSVAALLDKPSRRKVEVTIDYRGFEVPDFFVVGMGLDYAEKYRNLRDICILKPEIYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S59</accession>
    <name>Q67S59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH499"/>
    <dbReference type="EnsemblBacteria" id="BAD39484">
        <property type="protein sequence ID" value="BAD39484"/>
        <property type="gene ID" value="STH499"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH499"/>
    <dbReference type="HOGENOM" id="CLU_104063_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009665">
        <property type="entry name" value="DUF1256"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06866">
        <property type="entry name" value="DUF1256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02841">
        <property type="entry name" value="spore_YyaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="205" mass="22258" checksum="38874FF42D7F7E6E" modified="2004-10-11" version="1">MSRDLPRPAAGVPPLLDVHFEDPTAPERLAAAIARQFRALPHPERHLPVFFLVGATSSTGDSLGPFTGWFLRRKGFRGEHVGDLADPVHATNLRERLAEARARALRRERLPYIIAVDAAVGRPGRITVNRGPLRPGAAMGKALPQVGHLHIMGGTANFPFMIWFTDLDQTVGMAEVIADGLLAFWAAYESGELFGRPSVARTGLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S72</accession>
    <name>ARLY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">STH486</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194620.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S72"/>
    <dbReference type="SMR" id="Q67S72"/>
    <dbReference type="STRING" id="292459.STH486"/>
    <dbReference type="EnsemblBacteria" id="BAD39471">
        <property type="protein sequence ID" value="BAD39471"/>
        <property type="gene ID" value="STH486"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH486"/>
    <dbReference type="eggNOG" id="COG0165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_9"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="751464at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137836" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <sequence length="461" mass="51366" checksum="7368E45A2DA241E6" modified="2004-10-11" version="1">MTKLWGGRFTKPADKTAEGFTSSLAFDRRLYKQDIRGSIAHVRMLGRQGIIPAADAARIEQGLREIEAEIEAGQFPFRQEYEDIHLNIEKRLIEKIGPAGGRLHTARSRNDQVVTDVHLWVKDEIAAVQRLVSDLQGTLLDRAREQMGAVMPGYTHLQRAQPVLLSHHLMAYFWMLERDYGRFADALRRADVSPLGAGALAGTTFPIDREFTAAELGFAGVYPNSMDAVSDRDFIVEFVAAAAICQMHLSRLAEELVMWSSTEFGFVEMDDAYATGSSIMPQKKNPDVAELVRGKTGRIYGDLMALLTVLKGLPLAYHTDLQEDKERLFDAVDTLKACLTVMTGMLATLKFNRERMAQAVRRDFSNATDMADYLVKKGMPFREAHEVVGKAVLYCVERGKFLADLTLEEFKAFSALFEADIYQAIAPETCVSQRTSLGGTAPAEVERQLALAAEILSRRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD6</accession>
    <name>Q67SD6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH422</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194556.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH422"/>
    <dbReference type="EnsemblBacteria" id="BAD39407">
        <property type="protein sequence ID" value="BAD39407"/>
        <property type="gene ID" value="STH422"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH422"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037123_0_0_9"/>
    <dbReference type="OMA" id="EHGNRPF"/>
    <dbReference type="OrthoDB" id="1401629at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="8">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="8">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="30890" checksum="D2A7C7897FB709DA" modified="2004-10-11" version="1">MAGVRVEVHWLFLLALLVAAVSGYYLEALILLGSLAAHELAHLTVAWLLGVDIDSLVLTPFGGMARLDPSLDADPQSEVSVALAGPVQSFLLAGLASFLMGDRLFDPSLVRFFFQVNANLAFFNLIPALPLDGGRALRGLLAQRWGYRTTTVWMARLGLLSGAAMLAAALGVLAAAGQVFLTPLAGGLFLALGAMAEGEEAVLRSYQQFLQKRRRMARQRIIPGGTLVVVEGTRIGEVLERLSARRYHLVLVVDRSLLPVGTLHEAEILDAFEVLGPRATVEQVLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG1</accession>
    <name>Q67SG1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH397</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194531.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH397"/>
    <dbReference type="EnsemblBacteria" id="BAD39382">
        <property type="protein sequence ID" value="BAD39382"/>
        <property type="gene ID" value="STH397"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH397"/>
    <dbReference type="eggNOG" id="COG4965">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1030258_0_0_9"/>
    <dbReference type="OMA" id="FLNWQRQ"/>
    <dbReference type="OrthoDB" id="1515313at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="97"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="28770" checksum="73360670BBF10ED0" modified="2004-10-11" version="1">MRLLIGLLSAGAVFLALMPAGPRPEGVTLPGFLRPGPADRALRLVPVAAFALVLLLTGSWLTALISLPAGVLLLATVRDTLARREAQALRDQLQEALLSLATSLKAGQSLPRALQRCAVELSRLHPGGGGLIREIELTAREVELGTPVDEALLDLRARVPLEELAALVDALVTTRRRGGNIVEVMGNVAQMVADRLAVEREIQVLTAQKRTEAAILAMIPLGIYLVVRVTNPAYLAVFHATLWGQAALGLILLAVAGGYWMAQRIASIDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ8</accession>
    <name>Q67SQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative beta-N-acetylglucosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH300"/>
    <dbReference type="EnsemblBacteria" id="BAD39285">
        <property type="protein sequence ID" value="BAD39285"/>
        <property type="gene ID" value="STH300"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH300"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_5_3_9"/>
    <dbReference type="OMA" id="STDQEHG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_3" evidence="2">
        <location>
            <begin position="9"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="523" mass="54459" checksum="DD6E40BD84755129" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST6</accession>
    <name>Q67ST6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194407.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH272"/>
    <dbReference type="EnsemblBacteria" id="BAD39257">
        <property type="protein sequence ID" value="BAD39257"/>
        <property type="gene ID" value="STH272"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH272"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_11_0_9"/>
    <dbReference type="OMA" id="MRQTHIA"/>
    <dbReference type="OrthoDB" id="362657at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017895">
        <property type="entry name" value="HTH_IS408/IS1162_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50532">
        <property type="entry name" value="HTH_IS408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH IS408-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="124"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="508" mass="57885" checksum="A45D9B1391785992" modified="2004-10-11" version="1">MRKIREILRLKWEVGLSARQIARSLSISHSTVLDMLRRFECSGLSWPLPNIDDAELEAKLYPGNPQGLVERPLPDMAYIHRELARKGVTLQLLWWEYKQNHPDGLQYSQFCQRYRQWRSKLDVVLRQPHRAGEQMQVDFAGDTVPIHDRETGEVWQAPIFVAVLPASNYTFAKAYPAQDLRSWIKAHCDALEFFGGVPEIIVPDNPKAGVTRACRYEPDLNPAYQEMAAHYGAAVIPARPRSPRDKAKVENAVLVVERWILAALRNHTFFSLHDANRAIREAVARLNDRPFQKLEGTRRSLYETLDKPALRPLPPQRYEFAEWRKAKVNLDYHVEVDRNYYSVPYPLVGETVDIRLTDGLVEILHRGKRVACHPRATGRGRSVTQIQHMPASHRRHREWTPSKLRRWAESVGPHTAQLVVTILEEKPHPEQGFRSCLGIIHLARKYGSDRLEAAAARACAARTFSYSSVKSILAHNLDQAPLPGPPDNQATPVHPNLRGPDYYAGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY4</accession>
    <name>Q67SY4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate oxidoreductase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194359.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH224"/>
    <dbReference type="EnsemblBacteria" id="BAD39209">
        <property type="protein sequence ID" value="BAD39209"/>
        <property type="gene ID" value="STH224"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH224"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_0_1_9"/>
    <dbReference type="OMA" id="EVSWMPS"/>
    <dbReference type="OrthoDB" id="1777574at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="12"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="185" mass="19424" checksum="4369463209C6EBF2" modified="2004-10-11" version="1">MYTHEILIAGFGGQGVLTAGQLLAYAGNLEGQHVSWVPAYGPEQRGGTANCSVVISDQPVACPMVSEPTACIVLNQPSLEKFTPAVQPGGTLVVNTSLCTGRATRTDITVVPVPATDLAVELGNVRFANMIALGALLAVVPVVKVESILEVLPKVMGEDKARYIPINREALSRGMEEARRVLAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY6</accession>
    <name>Q67SY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate oxidoreductase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194357.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH222"/>
    <dbReference type="EnsemblBacteria" id="BAD39207">
        <property type="protein sequence ID" value="BAD39207"/>
        <property type="gene ID" value="STH222"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH222"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_0_0_9"/>
    <dbReference type="OrthoDB" id="360732at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="17"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="248"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="355" mass="38453" checksum="40576A451A72A0AA" modified="2004-10-11" version="1">MEPTTTMMMKGNEALGEAAIRAGCRFFFGYPITPQSELPAYMARRMPEVGGTFLQAESELAAVNMLLGAAATGVRAMTSSSSPGYSLKQEGISYMIGSQLPCVVVNIMRGGPGLGNIQPSQSDYFQIVKGGGHGDYHMIALAPSTVQELVDLTILAFDLADAYRNPVAIVGDGLLGQTMEPVSFPPVQTHAVEKDWALTGAAGRPKRIITSLELNPENLEAHNRVLQAKFSAMAEREKRWEEYRCDDADLVVVAYGTSARIARAAVDAARKQGLRVGLYRPITLWPFPGDGLARLAERGVRRFLDVEMSSGQMVEDVKLAVLGRARVDFYGRLGGMVPTPREILGQIQERMGGEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T83</accession>
    <name>SECA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="1">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secA</name>
        <name type="ordered locus">STH125</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2.</text>
            <dbReference type="EC" id="7.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">May bind 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194260.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T83"/>
    <dbReference type="SMR" id="Q67T83"/>
    <dbReference type="STRING" id="292459.STH125"/>
    <dbReference type="EnsemblBacteria" id="BAD39110">
        <property type="protein sequence ID" value="BAD39110"/>
        <property type="gene ID" value="STH125"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH125"/>
    <dbReference type="eggNOG" id="COG0653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005314_3_0_9"/>
    <dbReference type="OMA" id="MVHYDVQ"/>
    <dbReference type="OrthoDB" id="212453at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008564">
        <property type="term" value="F:protein-exporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000318465" description="Protein translocase subunit SecA">
        <location>
            <begin position="1"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="861"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="885"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="887"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="896"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="897"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="903" mass="102376" checksum="778C94AE578C9199" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE7</accession>
    <name>Q67TE7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative S-layer associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH61</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH61"/>
    <dbReference type="EnsemblBacteria" id="BAD39046">
        <property type="protein sequence ID" value="BAD39046"/>
        <property type="gene ID" value="STH61"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH61"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_647088_0_0_9"/>
    <dbReference type="OMA" id="WANTHIT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269674" evidence="2">
        <location>
            <begin position="25"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="21"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="86"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="152"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="424" mass="45447" checksum="AD6603B7C37993DD" modified="2004-10-11" version="1">MRSPVCRVGLIALLILLIPGAALAYLSDVAGHWSAVWLSALEARGIIAGDRAGRFYPDDPLTRAELARIAVTGLGYGTEALALQGVPSRFTDVAPDDPLAGYVELLWELGLTEGYPDGRFRPEAPLTRVELAAMLVRAAGLEQRAAVLRSARLSYRDAGEIPAWAVGAVAAAAEAGLMSGDGDGYYRPWALVTRAEGAAAVAKWLDQYGLLFDLEGTLVAFDPREGEGTVRTPMGEEHSISLAVDAAILRGGERVLPPALQVADQVSLILNDAGQVRFVEAWYADLLAEGLEPAGGNGVWATLGTGQRRRLWVQPGALVFVNGRPATLAEALGQGPTYMALDVKTEEVRYLDAVRTPFGGRVAVVEDDRIWLEDGDGFLRYDLASDAILVDAGRRVEFYEIAEGDQVAFALDGDGRITYLEIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF2</accession>
    <name>Q67TF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH56</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH56"/>
    <dbReference type="EnsemblBacteria" id="BAD39041">
        <property type="protein sequence ID" value="BAD39041"/>
        <property type="gene ID" value="STH56"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH56"/>
    <dbReference type="HOGENOM" id="CLU_2425875_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="10226" checksum="BF6DDBC46079F144" modified="2004-10-11" version="1">MLASLRATVLPQFTVEVYFDCEPDCRTHPRYVLVLRDGTAGAVELEFASARDLRAFLARSRQALTECRRTARIHRSPSKRVASGTVQATLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ7</accession>
    <name>Q67TJ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tadA</name>
        <name evidence="5" type="ordered locus">STH11</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194146.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH11"/>
    <dbReference type="EnsemblBacteria" id="BAD38996">
        <property type="protein sequence ID" value="BAD38996"/>
        <property type="gene ID" value="STH11"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH11"/>
    <dbReference type="eggNOG" id="COG0590">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_3_2_9"/>
    <dbReference type="OMA" id="PCQMCAG"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD38996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="16976" checksum="8BAEF8AF5877A639" modified="2004-10-11" version="1">MTDEAFMRAALAEAEKAARLGEVPIGAVIVRDSEILVRTHNLRETTHDATAHAEVLAIREAGRLLGGWRLTGCTLYVTIEPCPMCAGALLQSRIDRVVFGARDPKAWADRSILEFLQNPGLNHRVEVRDGVLAEACSEVIRQFFRERRRVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK1</accession>
    <name>Q67TK1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">gyrA</name>
        <name evidence="11" type="ordered locus">STH7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH7"/>
    <dbReference type="EnsemblBacteria" id="BAD38992">
        <property type="protein sequence ID" value="BAD38992"/>
        <property type="gene ID" value="STH7"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH7"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_6_1_9"/>
    <dbReference type="OMA" id="THHWLLF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="10">
        <location>
            <begin position="12"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="526"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD38992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="813" mass="91561" checksum="3169BD1017F97E86" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J87</accession>
    <name>Q67J87_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin converting factor small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197394.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3282"/>
    <dbReference type="EnsemblBacteria" id="BAD42263">
        <property type="protein sequence ID" value="BAD42263"/>
        <property type="gene ID" value="STH3282"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3282"/>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_9"/>
    <dbReference type="OrthoDB" id="1472912at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="9974" checksum="21B7B3106955A4EA" modified="2004-10-11" version="1">MQVRLYATLRALAGAARVEVPGTPGEPVGDVLRRLVAQCPGLEGQILTPDGAALLPHVQVFIAGRSIRDLQGMATPVPPGADLAVFPPVAGGCGLLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD9</accession>
    <name>Q67JD9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197342.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3229"/>
    <dbReference type="EnsemblBacteria" id="BAD42211">
        <property type="protein sequence ID" value="BAD42211"/>
        <property type="gene ID" value="STH3229"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3229"/>
    <dbReference type="eggNOG" id="COG1188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101003_4_0_9"/>
    <dbReference type="OMA" id="VANEACD"/>
    <dbReference type="OrthoDB" id="2006597at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025490">
        <property type="entry name" value="RNAbp_HP1423"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038881">
        <property type="entry name" value="RNAbp_HP1423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="97" mass="10889" checksum="E50D290A60C01E50" modified="2004-10-11" version="1">MRIDKFLKVSRIIKRRTLAKEVCDAGRVQVGGKVAKAGTEVKPGDTISIDFGRRQLTVRVLAVREHVRAAEARELYEVIAEEFKADPLLAELEDDPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG9</accession>
    <name>TILS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="1">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tilS</name>
        <name type="ordered locus">STH3199</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JG9"/>
    <dbReference type="SMR" id="Q67JG9"/>
    <dbReference type="STRING" id="292459.STH3199"/>
    <dbReference type="EnsemblBacteria" id="BAD42181">
        <property type="protein sequence ID" value="BAD42181"/>
        <property type="gene ID" value="STH3199"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3199"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018869_0_1_9"/>
    <dbReference type="OMA" id="PTNQDPA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012796">
        <property type="entry name" value="Lysidine-tRNA-synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11734">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00977">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02433">
        <property type="entry name" value="lysidine_TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000181786" description="tRNA(Ile)-lysidine synthase">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <sequence length="482" mass="52556" checksum="43A829C21C7839EE" modified="2004-10-11" version="1">MPLLDRVREYVVRHGMLEPGDRVIVAVSGGPDSLALTHVLYRLAPAWRLSLHLFHLDHGLRGEASRADAAFVADLARELSLPLTTVTLRPGELEAMRGSLEDNARRRRYAEMARLAAAIGAQRAATGHNRNDQAETVLMRLLRGSGSTGLAGIPPVRREGGLTIIRPLLGASRQEILAYCRAHGLTPRMDATNLQGDFERNRIRLEILPALTERFGDAVVDNLAQTADLLREEDRLLAELTREACARCGWRETGEATGEPVVELDGTLLVQEPLALARRIVRMAVQRVSGSAYGPGLSAVTRALELAGRTEGSHWLDLPQGVRLSVAYGRCRFARSHPQLGPGAEALEQVWPVAVPGETAIPALGVTVAAELVPPSAMPARLPDDEMWLDRDRLPGPLAVRTRRPGDRLWPAGMQGSKKLQDILVDAKVPRDQRDGLPLLVAGDTVVWVPGVIRDRRFRPDAGTRSAVRVTVRRRPEGSGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ4</accession>
    <name>Q67JJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase iron-sulfur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3174</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197287.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3174"/>
    <dbReference type="EnsemblBacteria" id="BAD42156">
        <property type="protein sequence ID" value="BAD42156"/>
        <property type="gene ID" value="STH3174"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3174"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_3_9"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="28323" checksum="0C3255E7EADB7529" modified="2004-10-11" version="1">MADQRKFIELRIRRQEAPDKPFYWEEFRIPYRPGMNVIACLMEIQRNPVNAEGKRTTPVVWDSNCLEEVCGACSMVINGKARQACSALVDNLTQPIVLEPLKSFPLVRDLMVDRSRMFEALKRVKAWIPIDGTHDLGPGPRQSPADQEWMYKLSECMTCGCCMEACPNYNEKTDFIGPAPISQVRLFNAHPTGKMHAHERLEALMGPGGIMECGSAQNCVRACPKGIPLTTSIAEIQRQLTVYAVKKMFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM7</accession>
    <name>Q67JM7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197254.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3141"/>
    <dbReference type="EnsemblBacteria" id="BAD42123">
        <property type="protein sequence ID" value="BAD42123"/>
        <property type="gene ID" value="STH3141"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3141"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_3_9"/>
    <dbReference type="OMA" id="LLIFDWD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="218" mass="23922" checksum="DBAB7C685DC76A90" modified="2004-10-11" version="1">MSHFDAVLFDLDGTLIDTNRLIVTSFQHVFRTRLGLEMAPEEIYRFFGEPLRTTMTRFAPDRADELTEAYREYNLSVHDRLVRRFPGVNDAVAALRQAGVRLGVVTSKYTPLARRGLSVCGLEAHFPVVVGEDQTERHKPEPDPALLALELLGVQPGPRVLMVGDSPLDLRCGRAAGCRTAAVGWALDRAALAAGEPDFWLERPSDLVALVLSGAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR3</accession>
    <name>Q67JR3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42087">
        <property type="protein sequence ID" value="BAD42087"/>
        <property type="gene ID" value="STH3105"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3105"/>
    <dbReference type="HOGENOM" id="CLU_2738576_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="6333" checksum="3C033D6B10D1DE0E" modified="2004-10-11" version="1">MAEGAAGVTGAIAGAALGSAAGPLGTVAGAIAGGLGGAAMANGAGNTTGAENQNGRGRNRAGDQAGQNPTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV1</accession>
    <name>RL29_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmC</name>
        <name type="ordered locus">STH3067</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197182.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV1"/>
    <dbReference type="SMR" id="Q67JV1"/>
    <dbReference type="STRING" id="292459.STH3067"/>
    <dbReference type="EnsemblBacteria" id="BAD42049">
        <property type="protein sequence ID" value="BAD42049"/>
        <property type="gene ID" value="STH3067"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3067"/>
    <dbReference type="eggNOG" id="COG0255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_5_2_9"/>
    <dbReference type="OMA" id="RFQMATS"/>
    <dbReference type="OrthoDB" id="2063644at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000130476" description="50S ribosomal protein L29">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <sequence length="70" mass="8199" checksum="B0C25007B194CE22" modified="2004-10-11" version="1">MKVKEIREMSTEEIKAKVAELKEELFNLRFQLAVNNLENTARIREVRRAIAQCKTVIRERELKAQGEVKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW6</accession>
    <name>IF12_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor IF-1 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">infA2</name>
        <name type="ordered locus">STH3052</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197167.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW6"/>
    <dbReference type="SMR" id="Q67JW6"/>
    <dbReference type="STRING" id="292459.STH3052"/>
    <dbReference type="EnsemblBacteria" id="BAD42034">
        <property type="protein sequence ID" value="BAD42034"/>
        <property type="gene ID" value="STH3052"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3052"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_1_0_9"/>
    <dbReference type="OMA" id="AHVSGKM"/>
    <dbReference type="OrthoDB" id="2066663at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000095888" description="Translation initiation factor IF-1 2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <sequence length="72" mass="8306" checksum="0D7BED4BCE2F95F6" modified="2004-10-11" version="1">MSKDDVIEVEGTVVENLPNAMFRVELPNGHRVLAHVSGKIRMHFIRILPGDRVTVELSPYDLTRGRIVYRYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY0</accession>
    <name>Q67JY0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197152.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3037"/>
    <dbReference type="EnsemblBacteria" id="BAD42020">
        <property type="protein sequence ID" value="BAD42020"/>
        <property type="gene ID" value="STH3037"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3037"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_16_1_9"/>
    <dbReference type="OMA" id="DAYHEFE"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="41"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="21695" checksum="3B1A8AF0220F57CA" modified="2004-10-11" version="1">MVHRFDPQRVERLLDERRYSWHDPAQVLRHLDLDRCTALADVGCGPGWFALPAAKRLPPGGVVYGIDISEEMLEHLRRRARAQGVDNVVPVLAEEEDEWPVPTESCEAVLIADVYHEVDPASMFIGEVKRILRPGGVCLVVDWKPEPTPEGPPADHRVDPDDVIAEFTAGGFQFVGPCDVGPYSYGLKFAKPPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K47</accession>
    <name>Q67K47_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2968</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2968"/>
    <dbReference type="EnsemblBacteria" id="BAD41951">
        <property type="protein sequence ID" value="BAD41951"/>
        <property type="gene ID" value="STH2968"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2968"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362043_0_0_9"/>
    <dbReference type="OMA" id="PADPIYF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="649" mass="72661" checksum="58148DA2D20D2E8B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K55</accession>
    <name>Q67K55_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197075.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41943">
        <property type="protein sequence ID" value="BAD41943"/>
        <property type="gene ID" value="STH2960"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2960"/>
    <dbReference type="HOGENOM" id="CLU_2756366_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="70" mass="7406" checksum="30EDC2067796775C" modified="2004-10-11" version="1">MKPDPRVTPTGDAAADNPAISRLTVERLGMELATELGVDPGRIGALGASPDPAVQRYEAETHSPKSRPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K65</accession>
    <name>Q67K65_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YceI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0312 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197066.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2950"/>
    <dbReference type="EnsemblBacteria" id="BAD41933">
        <property type="protein sequence ID" value="BAD41933"/>
        <property type="gene ID" value="STH2950"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2950"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_3_2_9"/>
    <dbReference type="OMA" id="GNYRAGF"/>
    <dbReference type="OrthoDB" id="1767227at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YceI" evidence="2">
        <location>
            <begin position="5"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="19208" checksum="398C8599A74895BB" modified="2004-10-11" version="1">MAKSVWVVDNSHSLVEFAVRHMMIATVKGRFTQVEGRIEVDPADITGAYFEGSVPVASINTADSARDDHLRSADFFDAQNYPTLTFKSTKVERAGDGYKLTGDLTIRGVTKPVTFDLEFAGVGKDPWGNEKIGFSATGKINRKDFGLTWNAVLETGGVLVGEEVKIELHLEAARQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD3</accession>
    <name>ARGB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylglutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAG kinase</fullName>
            <shortName evidence="1">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argB</name>
        <name type="ordered locus">STH2882</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196999.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KD3"/>
    <dbReference type="SMR" id="Q67KD3"/>
    <dbReference type="STRING" id="292459.STH2882"/>
    <dbReference type="EnsemblBacteria" id="BAD41865">
        <property type="protein sequence ID" value="BAD41865"/>
        <property type="gene ID" value="STH2882"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2882"/>
    <dbReference type="eggNOG" id="COG0548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_9"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="OrthoDB" id="901370at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112674" description="Acetylglutamate kinase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="65"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <sequence length="293" mass="30825" checksum="8657D9B651CE8F0D" modified="2004-10-11" version="1">MDQQALDKAAVLAEALPYIREFSGKTVVIKYGGAAMAAADLKAAVMQDIALMKYVGMHPIVVHGGGPEVSELARRMGIEPQFVDGLRVTDAATMEIAQMVLVGKTNREIVTHLCAQGVKAVGLSGQDAGLIRAARHLHRSRETGEMVDLGFVGDVAAVDTEVLTTLTTAGYVPVIAPIGVGPGGQPYNINADTVAGAIAAAMKAEKLVLLTDVEGVRADKDDPSSLLSRVTAQEVKSWIARGRLQGGMIPKLQCCLTALEGGVNRVHIIDGRVPHSLLLEIFTDEGVGTMVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD8</accession>
    <name>Q67KD8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DinB_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2877"/>
    <dbReference type="EnsemblBacteria" id="BAD41860">
        <property type="protein sequence ID" value="BAD41860"/>
        <property type="gene ID" value="STH2877"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2877"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979716_0_0_9"/>
    <dbReference type="OMA" id="WENDEWM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="1">
        <location>
            <begin position="142"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="31132" checksum="58066C715F305241" modified="2004-10-11" version="1">MSLSEPNARPPQALLYVQDLPRAAAYYAGLPGFRAVPAGSADVLVTAPEGETLVLLRAGTEPPPALAAPPAGPGAWVYLHRRDLPDLAARLRQAGLMPDGPTVTYPGYRQMLLPDPEGYVLAFWEELPVTDDQILELYRTGPARLADAVGRLGDAGLDVPRAPGKWTARQIVHHIVDSDAETFHVLRIALALPGRRIATTVWENDEWMRGLACDRRAVEPAIALFAAARAWILEALSHLPDALERTVTWPSGYTASVRDLLRQQGGHAVHHILQLEEMQRRLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ2</accession>
    <name>GATA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="1">Glu-ADT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatA</name>
        <name type="ordered locus">STH2821</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KJ2"/>
    <dbReference type="SMR" id="Q67KJ2"/>
    <dbReference type="STRING" id="292459.STH2821"/>
    <dbReference type="EnsemblBacteria" id="BAD41806">
        <property type="protein sequence ID" value="BAD41806"/>
        <property type="gene ID" value="STH2821"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2821"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_9"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241162" description="Glutamyl-tRNA(Gln) amidotransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <sequence length="488" mass="51669" checksum="38C1D0EB1AEC1D95" modified="2004-10-11" version="1">MLSAARLNRLFLAGELSAVEIAESALSRIAQVEPAVGAFITVAADHVIERAKKLDARRKAGDTELGPLAGVPIAVKDNICTSGMETTCASRILKGYVSPFDATVVERLRAAGAMIIGKANMDEFAMGSSGESSAFGVTRNPWDLERVPGGSSSGSAAAVAAGEAPLALGTDTGGSIRQPAAFTGIVGLKPTYGYVSRYGVVAFASSLDQVGPMGRDVEDVARLFEVIAGPDRRDATNAGRTPPALKFGGEPSLSGVRLGVPKELLGPGIDPGVKARVEEAIAQLEELGATVEECSLPSTEYALSAYYVIAVAEASSNLARFDGVRYGYRAAQAGGLHEMYSKTRGEGFGTEVKRRIMLGTYVLSAGHYDAYYRRAQQVRTLVVRDFERAFERYDALVTPTTPFTAWKIGEKVDDPVSMYLGDICTIPVNLAGLPAVSVPCGFVDGLPVGMQLIGKPFADTQILQIAWAYQKVTKHHEARPALTEEGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ7</accession>
    <name>Q67KJ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 5-formyltetrahydrofolate cyclo-ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196935.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2816"/>
    <dbReference type="EnsemblBacteria" id="BAD41801">
        <property type="protein sequence ID" value="BAD41801"/>
        <property type="gene ID" value="STH2816"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2816"/>
    <dbReference type="eggNOG" id="COG0212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031500_2_0_9"/>
    <dbReference type="OMA" id="KTVYMAV"/>
    <dbReference type="OrthoDB" id="1816478at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="PTHR13017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="27458" checksum="08ADC14B5989E0AA" modified="2004-10-11" version="1">MEKGQIRQWVWDELTRRGQAAFPKPVHGRIPNFVGSAAAAARLRELEVYRQARVVKVGPDSPLHPIRAMVLRDGKKLYMPTPRLTAGFVVLEGVEPGREREATSLTNFRRFGREVRLEEIDPIDLVVAGTVAVSQSTGVRIGKGAGYGDMEFALLCRLGKLRPDTPVVTAVHDLQVVDAPDHPLGVELPWDPHDISVDYIVTPTRTIAVRRAHPQPTEIDWSLLEPERLRALQPLLELRRMQGGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK3</accession>
    <name>Q67KK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ubiquinone/menaquinone biosynthesis methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196929.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2810"/>
    <dbReference type="EnsemblBacteria" id="BAD41795">
        <property type="protein sequence ID" value="BAD41795"/>
        <property type="gene ID" value="STH2810"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2810"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_7_3_9"/>
    <dbReference type="OMA" id="VNWAARH"/>
    <dbReference type="OrthoDB" id="1865374at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="205" mass="22758" checksum="D1012EADAB26A02A" modified="2004-10-11" version="1">MDRKRKTDALRRKLDRNARWFDMGAGYAPWRERLVREARGRVLEIGVGTGHNLPFYHPSVTTELVGIDLSPGMLARARSKPCRVPVTLLEMDAQEMAFPDASFDTVVASYVFCTIPDPVRALREAGRVCRPDGRILLLEHVRIDRPIIGPLMDLAAPLVAGIIGCHINRRTVENVRLAGLKVEKVEPVRGDLLKLIHARPGAGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP4</accession>
    <name>Q67KP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADH dehydrogenase I subunit L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nuoL2</name>
        <name evidence="10" type="ordered locus">STH2769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196888.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2769"/>
    <dbReference type="EnsemblBacteria" id="BAD41754">
        <property type="protein sequence ID" value="BAD41754"/>
        <property type="gene ID" value="STH2769"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2769"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_0_9"/>
    <dbReference type="OMA" id="GFWYREV"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="666"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="9">
        <location>
            <begin position="79"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="8">
        <location>
            <begin position="145"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="685" mass="75884" checksum="C340C257E694EC6D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP8</accession>
    <name>Q67KP8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2764"/>
    <dbReference type="EnsemblBacteria" id="BAD41749">
        <property type="protein sequence ID" value="BAD41749"/>
        <property type="gene ID" value="STH2764"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2764"/>
    <dbReference type="HOGENOM" id="CLU_2083670_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="117" mass="12838" checksum="746684B4A66DB441" modified="2004-10-11" version="1">MGVTTGQEVKGVPAVLFLHGLQWLLAYLSLDLVFRTVDVRPGLLARSRARCRRRYVLAAAAATLVIGAAQVWTAGGAGAAAAGTAGLGWLPWWLLWRFGWRKQFPHLYQEPPEGKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ5</accession>
    <name>Q67KQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196876.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2757"/>
    <dbReference type="EnsemblBacteria" id="BAD41742">
        <property type="protein sequence ID" value="BAD41742"/>
        <property type="gene ID" value="STH2757"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2757"/>
    <dbReference type="HOGENOM" id="CLU_1767111_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="15801" checksum="7A5D1FF472757F18" modified="2004-10-11" version="1">MRSWMLHLMAIGGGAALLQAARGFQQLVVDPRTFTYAWGYFFLAVALQVLAGLVFLLPVLWARVRGEGGVRPHWGLVALYVLAGLVLAFLQPLQIVLGRSGFIAAADFVRILPTGTSPNIFGAFLIAIGIVYAVGRGPEPGQKPAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L19</accession>
    <name>Q67L19_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarate hydratase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196764.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2642"/>
    <dbReference type="EnsemblBacteria" id="BAD41627">
        <property type="protein sequence ID" value="BAD41627"/>
        <property type="gene ID" value="STH2642"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2642"/>
    <dbReference type="eggNOG" id="COG1838">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098588_2_0_9"/>
    <dbReference type="OMA" id="IYHCGPL"/>
    <dbReference type="OrthoDB" id="1627779at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase_C" evidence="3">
        <location>
            <begin position="10"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="191" mass="20439" checksum="7F3903309C5E1354" modified="2004-10-11" version="1">MAKHYVTTPISPEQAKAIRSGDEVYITGTIYTARDAAHKRMVEDLEAGRPLPFDPQGAVIYYVGPTPPKPGQVIGSAGPTTSYRMDKYTPTMLKLGVKAVIGKGYRGPEVKSALVENGALYLAATGGAGALLAKRIEAAEVVAYEDLGPEAIRKLTVKDFPAICIHDAHGGDLYRDGQSEWVAPDAMVKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L24</accession>
    <name>Q67L24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2637</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2637"/>
    <dbReference type="EnsemblBacteria" id="BAD41622">
        <property type="protein sequence ID" value="BAD41622"/>
        <property type="gene ID" value="STH2637"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2637"/>
    <dbReference type="eggNOG" id="COG2009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127125_1_0_9"/>
    <dbReference type="OMA" id="WSWVAHR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045281">
        <property type="term" value="C:succinate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039023">
        <property type="entry name" value="SdhC_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41910">
        <property type="entry name" value="PTHR41910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="15962" checksum="0DD5FAFFD86DC9FC" modified="2004-10-11" version="1">MARVNTPPNRPVRSFADLNPRYFRTGMWSHVLHRITGVAITVFLLLHIWEITSVVRGGAAGFDTKMAGMAAKLWVVGEWLLFLALVFHAINGIRLILLDLGWGVREQKRNFWVVFILSAVIITIGSYFFVMRFLAYPWAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L52</accession>
    <name>Q67L52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196731.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2609"/>
    <dbReference type="EnsemblBacteria" id="BAD41594">
        <property type="protein sequence ID" value="BAD41594"/>
        <property type="gene ID" value="STH2609"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2609"/>
    <dbReference type="eggNOG" id="COG4152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="TQRFSYW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="300" mass="33229" checksum="9C034DACA7816EED" modified="2004-10-11" version="1">MTLQVLDVYKAFGPVRAVQGVSFTVPAGRVCGLLGSNGAGKTTTMRMIMRIFLPDRGEIQWKGRPVTDAIRSTFGYLPEERGLYPKMQVREQLIYLTQLKGLSHAEAARQVDRWIERLELGEYALRRVEQLSKGNQQKVQFAAAAASRPELCILDEPFTGLDPMNTLVMEQAFRTLAREGTTILFSSHNMDQVEALCDDVVLLHRSRVVLAGTLREVKRAARRQTVRLRVDSGDYSFLREFPGIRTQSAAGGAVEVAVPDTLDPNQLLARAMSAGTVTEYSLGEPSLREIYIEKVGGADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L91</accession>
    <name>Q67L91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196692.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2570"/>
    <dbReference type="EnsemblBacteria" id="BAD41555">
        <property type="protein sequence ID" value="BAD41555"/>
        <property type="gene ID" value="STH2570"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2570"/>
    <dbReference type="eggNOG" id="ENOG50330BT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171794_1_0_9"/>
    <dbReference type="OMA" id="HEEPQNG"/>
    <dbReference type="OrthoDB" id="2069623at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021637">
        <property type="entry name" value="DUF3243"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024702">
        <property type="entry name" value="Uncharacterised_YmfJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038292">
        <property type="entry name" value="YmfJ/YflH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11588">
        <property type="entry name" value="DUF3243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004764">
        <property type="entry name" value="YmfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="92" mass="10575" checksum="99144B504CE14E1B" modified="2004-10-11" version="1">MENVSSDTPILESFEQWKQFLSDRVKEFKRTGASQQTITNMATSIGNFLAEKVDPRNREQRLLKELWDVGTEEERKALASMVTKMVSDGKVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA7</accession>
    <name>Q67LA7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2554"/>
    <dbReference type="EnsemblBacteria" id="BAD41539">
        <property type="protein sequence ID" value="BAD41539"/>
        <property type="gene ID" value="STH2554"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2554"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026152_3_0_9"/>
    <dbReference type="OMA" id="FNRIHWR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="2">
        <location>
            <begin position="181"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="419" mass="46154" checksum="01B78C8A1F11785C" modified="2004-10-11" version="1">MVLVAFIVARLGGGRLPYFVFYLSLLLWAGAWLYVRRVAGRVTGSVTVDRRSLEVGDEIPVKLRLENESLLPVSWVEVEDDTPAHLLASDRPRLGTTLPWVGTCIVHLTLTARRRGKCRVGPFRVRIRDWLGLFVREVVVASKAPVTIYPRVHPIDELPVPLAQPFGPVRTQERAFEDPSNPADIRRYVPGDNPRHIHWRTSARMGTLMVRQHELNATTQLILFPDFNREANVDACEAGGGSTADTAAEIAASLAALGVRRRMETGLFCVGQTRFAVSPGKGERVFLEIMEALAQVDAAGDVLLEQVLQAEAGHLGERATLVAITPRLTPGLADRLVALRARHRVTLILLDATTFAPPGLARRQIRRSDPALVDLLARRGVWVYVVPAGADLRRLDALRVRVGEGVGPWQPLARPQATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB2</accession>
    <name>Q67LB2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196672.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2549"/>
    <dbReference type="EnsemblBacteria" id="BAD41534">
        <property type="protein sequence ID" value="BAD41534"/>
        <property type="gene ID" value="STH2549"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2549"/>
    <dbReference type="HOGENOM" id="CLU_1884755_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14338" checksum="8C8ED57A81DB57A5" modified="2004-10-11" version="1">MSGMEPQGRGRAERLGPLVITVLFSLPLWADPVAQALGYDVFYLADPKLQAVYATLVQLLGGWPLYARAVRGAAARRFGAAGLPVLASSLLYAGGLVAAVRNVPAILWFLAAGVALIVGHAVEIRGRRAVSEMRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB8</accession>
    <name>MDH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mdh</name>
        <name type="ordered locus">STH2543</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196666.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LB8"/>
    <dbReference type="SMR" id="Q67LB8"/>
    <dbReference type="STRING" id="292459.STH2543"/>
    <dbReference type="EnsemblBacteria" id="BAD41528">
        <property type="protein sequence ID" value="BAD41528"/>
        <property type="gene ID" value="STH2543"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2543"/>
    <dbReference type="eggNOG" id="COG0039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_1_9"/>
    <dbReference type="OMA" id="CYIIVLT"/>
    <dbReference type="OrthoDB" id="870724at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01339">
        <property type="entry name" value="LDH-like_MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00487">
        <property type="entry name" value="Malate_dehydrog_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011275">
        <property type="entry name" value="Malate_DH_type3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01763">
        <property type="entry name" value="MalateDH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000113474" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00487"/>
        </source>
    </evidence>
    <sequence length="315" mass="33229" checksum="8277B3A4A25CDF1A" modified="2004-10-11" version="1">MKRKKIAIVGSGFTGQGTALFAAARDLGDIVLVDLPARENYAKGVALDMMEAMPVYGSDTRLVGTSDYAEIAGADVVVITAGVPRKPGMSREDLVNTNAGIVKDVAEKVARYAPDSVIIVLTNPVDSMTYVALKASGFPKNRVIGQSGVLDTARFRTFLANAIGCSFQDVVGCVLGGHGDDMVPLVRYTSAGGIPVEKLLPKETIDAIVERTRKGGGEIVNLMGTSAGYAPGMALVEMIDAILNDRKRILPSIAYLEGEYGYTDMCLGVMTVLGGGGLEKVIELDLTDEEKVALDKGAQGVRNLIEMLKAGILAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD5</accession>
    <name>Q67LD5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Phosphate propanoyltransferase</fullName>
            <ecNumber evidence="4 7">2.3.1.222</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2526</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in 1,2-propanediol (1,2-PD) degradation by catalyzing the conversion of propanoyl-CoA to propanoyl-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>phosphate + propanoyl-CoA = CoA + propanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:28046"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:58933"/>
            <dbReference type="EC" id="2.3.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Polyol metabolism; 1,2-propanediol degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PduL family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.222" evidence="4 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196649.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2526"/>
    <dbReference type="EnsemblBacteria" id="BAD41511">
        <property type="protein sequence ID" value="BAD41511"/>
        <property type="gene ID" value="STH2526"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2526"/>
    <dbReference type="eggNOG" id="COG4869">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080676_1_0_9"/>
    <dbReference type="OMA" id="EMRERPI"/>
    <dbReference type="OrthoDB" id="1156172at2"/>
    <dbReference type="UniPathway" id="UPA00621"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051144">
        <property type="term" value="P:propanediol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008300">
        <property type="entry name" value="PTAC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39453">
        <property type="entry name" value="PTHR39453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06130">
        <property type="entry name" value="PTAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010130">
        <property type="entry name" value="PduL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="20587" checksum="871C48E065D48383" modified="2004-10-11" version="1">MAEARTVVINVSARHVHLSPEHLEVLFGPGYQLTVKKHLVQPGQFAAEETVAVIGPKGSFPRVRVLGPVRGKSQLEISATDARTLGVPAVVRLSGNIAGTPGIVLEGPKGRVELTEGVIVAQRHIHMTPADAERYGVTDGQVVRVKTRGTRPLTFDDVVIRAREDMALEMHIDTDEANAAGIKDGDIGEILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE2</accession>
    <name>Q67LE2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196642.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41504">
        <property type="protein sequence ID" value="BAD41504"/>
        <property type="gene ID" value="STH2519"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2519"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="OrthoDB" id="1752733at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="40269" checksum="CCF1FB3BFADE749E" modified="2004-10-11" version="1">MLTEVAVPHQLLDDGELTDSARVLWCYLRAIRKQKTRLTWAELRRVTGLSQYRLKQHMRALDALRWIQLDSLGTREFECCALWRGTRAFTIPVDLVLDRRIPHGAKWLWAHIRGVGEFDYAQLQHVMRCSRISLRKYVRILSHYGWLVGEVVRAGRCKVYRFETVNIIDENRHADVQDFYEGLRLARLKERYSVGQYLLKCIVAVLVVDQLLIENGEVLRLVNPFTGGQLHYDLLLPVSRVALEFQGPQHYRTTQRYPSDTQLQEQRMRDDIKRRFSETHGITLIEVRADDLSFGTISSLLRRHNVPIRHSLDDQRHLCDAIEAEAANYRRWAQRLERRLSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI2</accession>
    <name>Q67LI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2479"/>
    <dbReference type="EnsemblBacteria" id="BAD41464">
        <property type="protein sequence ID" value="BAD41464"/>
        <property type="gene ID" value="STH2479"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2479"/>
    <dbReference type="HOGENOM" id="CLU_3158620_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="48" mass="5425" checksum="A8D6830A0CBA555B" modified="2004-10-11" version="1">MHKNMHTKTQRPAAPPETERPAGKCPQVHVEEHVIEEILRAAGLEELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI5</accession>
    <name>Q67LI5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cyclase/dehydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2476"/>
    <dbReference type="EnsemblBacteria" id="BAD41461">
        <property type="protein sequence ID" value="BAD41461"/>
        <property type="gene ID" value="STH2476"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2476"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_952729_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="56"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="33046" checksum="017A767E7E4C8BD4" modified="2004-10-11" version="1">MLYSWGADRPAGRPARPKQTEVLSHVSNQPVSLRIEQYLPSVTLVTRPLPGSSDFPVTAAQVTGQRHRVVVDTLIRPADMAPFAGATLVIYTHFDWDHCWGTAAFPGVPVIGHQLTRERLLAPEPGEFLERMRAEKPAHFEGSQIIPPDVTFRDRLDIDAGGLTLELHHLPGHTADSVVVHVPELGLLLAGDAVETPLPTLSESGHIRAWAGALRKWSQAGLKHVVPGHGRPGGPELLAANAAYIEGVLEAAEAGLAKGQTPQEIAAERPLAALLPAGLVDTLAPYYRQQHEANIAAAHAELRQSAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ7</accession>
    <name>Q67LJ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2464</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. ArgK/MeaB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196587.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2464"/>
    <dbReference type="EnsemblBacteria" id="BAD41449">
        <property type="protein sequence ID" value="BAD41449"/>
        <property type="gene ID" value="STH2464"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2464"/>
    <dbReference type="eggNOG" id="COG1703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043725_2_2_9"/>
    <dbReference type="OMA" id="RIRMTAH"/>
    <dbReference type="OrthoDB" id="1134430at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005129">
        <property type="entry name" value="GTPase_ArgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00750">
        <property type="entry name" value="lao"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="49"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="403" mass="43387" checksum="0252A95C8BBBCD3C" modified="2004-10-11" version="1">MRPEDRAAELFARLRSGDRRALARAITWIENGDEEAVALLRLVYPLTGRAHVVGVTGPPGAGKSTLVDRLAAEQRARGRTVAIVAVDPTSPFSGGAILGDRIRMQRTLTDPGVFMRSLAARGHLGGLSPATGEVVSVLDAFGFDVILVETVGAGQSEVEIMALAHTTCVVVVPGLGDEIQAIKAGILEIGDVFVCNKADRDGADRTVSEIEMMLDLGHMGRPGINRWPAGEDGNRNADAAAAGDRALSQDGEGPSLPPGAEEALRRLDRTGHHSAEELRYAARFMTRAAAHAAARHGTARPGDVSWRPPVVKTVAKDGQGVDAVVDRLDEHLAFLRETGRWEARREQDAAQRLQELVGQRAAQATLERARAAGDFEELVRQVAERRLDPYTAAEEIMRNHWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP3</accession>
    <name>Q67LP3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2418</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41403">
        <property type="protein sequence ID" value="BAD41403"/>
        <property type="gene ID" value="STH2418"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2418"/>
    <dbReference type="HOGENOM" id="CLU_2636743_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8303" checksum="728FAC79DBD47ABB" modified="2004-10-11" version="1">MKVRRSWVAVAVPSGLLQVRSSRLRARGTTNFHLAGGRSGSSPWVDRRCRAERVTSGMKGTPGGAKCDGFSLGWLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP4</accession>
    <name>Q67LP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative muramoyltetrapeptide carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2417</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S66 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2417"/>
    <dbReference type="MEROPS" id="S66.001"/>
    <dbReference type="EnsemblBacteria" id="BAD41402">
        <property type="protein sequence ID" value="BAD41402"/>
        <property type="gene ID" value="STH2417"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2417"/>
    <dbReference type="eggNOG" id="COG1619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034346_3_1_9"/>
    <dbReference type="OMA" id="VHSHTAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027461">
        <property type="entry name" value="Carboxypeptidase_A_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027478">
        <property type="entry name" value="LdcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040449">
        <property type="entry name" value="Peptidase_S66_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040921">
        <property type="entry name" value="Peptidase_S66C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003507">
        <property type="entry name" value="S66_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237">
        <property type="entry name" value="PTHR30237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02016">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17676">
        <property type="entry name" value="Peptidase_S66C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028757">
        <property type="entry name" value="LD-carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141986">
        <property type="entry name" value="SSF141986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S66" evidence="5">
        <location>
            <begin position="17"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S66C" evidence="6">
        <location>
            <begin position="201"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR028757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="34755" checksum="06AD9E0FB959EC44" modified="2004-10-11" version="1">MAVGVVKPPRLRRGDLVGVVAPSGPVDARREAVEAGIRRLEAYGFRVRTGEALWAREGVRAGARETQLADLHAMWADPEVKAIFCATGGVTAITLVDGLDYDLIAQNPKPFIGMSDITLLQAAMLRRTGLVSLHASGLADGLGSPDAELEAPYLLRLLTNPDPPGPLPDWAGEVRPVRPGRAESAGHDRGGASGRQDSGVRGRLVGGCCNCVLHLLGTSYWPDTEGAILFLEGVNLLTSAVLRGLAQLRLAGALDGVKAILFGHMEGCFRDRPSPLEGMALAVTEALGDLDLPVYQTEAFGHCVPNVTLPIGAVACIRDGRLVLEEGAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR9</accession>
    <name>Q67LR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Soprulation specific penicillin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2392</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transpeptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2392"/>
    <dbReference type="EnsemblBacteria" id="BAD41377">
        <property type="protein sequence ID" value="BAD41377"/>
        <property type="gene ID" value="STH2392"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2392"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_0_9"/>
    <dbReference type="OMA" id="IVMESKT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PASTA" evidence="4">
        <location>
            <begin position="588"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="4">
        <location>
            <begin position="650"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="713" mass="76976" checksum="D7620AB955722FBB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT7</accession>
    <name>Q67LT7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2374"/>
    <dbReference type="EnsemblBacteria" id="BAD41359">
        <property type="protein sequence ID" value="BAD41359"/>
        <property type="gene ID" value="STH2374"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2374"/>
    <dbReference type="HOGENOM" id="CLU_1609936_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="16294" checksum="AF28A3B1AA5E4F10" modified="2004-10-11" version="1">MSVGLPGVPTRLRGGGPAARPGGRAGGGRTHSGAGSAGGAAPENGQGGLAAWVKDRAAGLTALFTLSVPVLLAVGGAALVSGALYELVVGGRLFNWVEAVIGSVIGGISAGVGGWLPGSGSQPPTSRRVERRVWSATWSTPRSAPSAARWAPLPRVASPPACSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV6</accession>
    <name>Q67LV6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2355"/>
    <dbReference type="EnsemblBacteria" id="BAD41340">
        <property type="protein sequence ID" value="BAD41340"/>
        <property type="gene ID" value="STH2355"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2355"/>
    <dbReference type="eggNOG" id="COG0467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_573249_0_0_9"/>
    <dbReference type="OMA" id="GEHIYRI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030665">
        <property type="entry name" value="KaiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039117">
        <property type="entry name" value="KaiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="KaiC" evidence="2">
        <location>
            <begin position="3"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="466" mass="50986" checksum="5942E3D973657EB2" modified="2004-10-11" version="1">MHARLSTGVEGLDSVLGGGLFPASATLVRGAPGTGKTTLGIQFLCEGARRGEGGAYFTFEEFPEQIYRDARELGFDLQALEQAGKLQVITLDPGAFLDEAIRPGGLLEELKREIGLRRVVVDSVTLLELALAEKGGRRTLYLLRNALHRMGATSLLVEEGAPAEGPSAAEYMVDTVLHLTYDDFEVNRLRHLEVRKHRGSPFRTGKHVFVISRRGIRVLPALDRVPDLTVGEVVPTGLDRLDECLGGGIPRGSTLVLNVNSKCNYRYLLGAIAAAHLRQGDGYVAVCSAVRPPAVVAMVLSLYGFDAARLGAEGRWRYIEQEGRPVPPELEPYIIRVSNEFDLFVSPGSATAIAHGIGSPDGPRHWLWSLDLNAALHTLKQDAVVKGWAEAISHARHRGDTIVGICNFEEMGPAMSAFVQRNAEGILRTWFDGRYQYLQVQKAPNGRVSELMVIEYTDAPPYLALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV7</accession>
    <name>Q67LV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196477.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2354"/>
    <dbReference type="EnsemblBacteria" id="BAD41339">
        <property type="protein sequence ID" value="BAD41339"/>
        <property type="gene ID" value="STH2354"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2354"/>
    <dbReference type="HOGENOM" id="CLU_2620759_0_0_9"/>
    <dbReference type="OrthoDB" id="2048232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8973" checksum="C104C3592FBC047E" modified="2004-10-11" version="1">MTADLNAVRLEAALYFQRNPFARETPESLALRLGRPETLVEPALGQLVELQVLERRGRLYRYCRPYVAPAWDEAPSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ4</accession>
    <name>Q67LZ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196440.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41302">
        <property type="protein sequence ID" value="BAD41302"/>
        <property type="gene ID" value="STH2317"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2317"/>
    <dbReference type="HOGENOM" id="CLU_2866164_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="8138" checksum="A2724EDC7F8188F0" modified="2004-10-11" version="1">MTMPFGWGRDDDWWDDDWDGMGRGNPWRRRRRRRRFPSFGKFPFQKFNKFPWGKSPWGKWGKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M63</accession>
    <name>Q67M63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41232">
        <property type="protein sequence ID" value="BAD41232"/>
        <property type="gene ID" value="STH2247"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2247"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="671" mass="71695" checksum="C3121844A715FA9B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M74</accession>
    <name>Q67M74_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe-ADH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2234"/>
    <dbReference type="EnsemblBacteria" id="BAD41219">
        <property type="protein sequence ID" value="BAD41219"/>
        <property type="gene ID" value="STH2234"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2234"/>
    <dbReference type="HOGENOM" id="CLU_2720846_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="7677" checksum="3C1D65BA22E50A0A" modified="2004-10-11" version="1">MIWAAPGAAAQLPDEARRLGATRALLVSDRGLLATGTPERLQRMLEGAGVRCAVYADVMAEPSAEWLDPVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB9</accession>
    <name>Q67MB9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2189</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2189"/>
    <dbReference type="EnsemblBacteria" id="BAD41174">
        <property type="protein sequence ID" value="BAD41174"/>
        <property type="gene ID" value="STH2189"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2189"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_6_9"/>
    <dbReference type="OMA" id="VRRMDPE"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="41"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="30072" checksum="5CF8A450BEB37D01" modified="2004-10-11" version="1">MIQASVSQPVAAGEGAHVLQSQNRAEPSSQRRPAGARERGELTRDRLLEAAEEVFAEQGFFRASIAEITRRAGVAQGTFYLYFPAKEEIFRELVRKMSHDLRRTLQEAVAPIIDRRDAERVGLETFLKFVLERRNLYTVMRECEFVDPELHRWYYRRLAEGYVRGLRQAMARGQVRQLDPEALAYFLMGAAQMIGMRWTFWENTLPPREVIETVVSVILHGIDPAGLPREPRATPGTSEGHSPGAPDADPPAAPGADAPGPEPEAGPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC3</accession>
    <name>Q67MC3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat-shock protein Hsp33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196310.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2185"/>
    <dbReference type="EnsemblBacteria" id="BAD41170">
        <property type="protein sequence ID" value="BAD41170"/>
        <property type="gene ID" value="STH2185"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2185"/>
    <dbReference type="eggNOG" id="COG1281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054493_1_0_9"/>
    <dbReference type="OMA" id="FIVTECE"/>
    <dbReference type="OrthoDB" id="1406579at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="286" mass="30918" checksum="EA0A42D5E6CDCCFA" modified="2004-10-11" version="1">MENYIIKSLGFHRNVRILFAETTGIARAVCRQGQVDGAALRKALATTVTAAGLLCGTLKDRQRLTLKVGTSRPGCRIFAEVDADGNVRGYLSDEWRSVPAEEAAGAPLQRLIGDRGQLQVIRDLGMYRGFSGITDMPYGNIVDDLAHYFRQSEQTTTRFVVHVALAEGGEVAACRGAMAQLLPGADHALLGEIEQVLCDPRMADPGNPVTAWPKLLPGDAAVLGMAPLRASCGCSREMFLPMLRSLSPADLREAVATNRSLEVVCHACGCRYSFSPDEMAAWVPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC9</accession>
    <name>Q67MC9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycerol-3-phosphate ABC transporter substarate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2179"/>
    <dbReference type="EnsemblBacteria" id="BAD41164">
        <property type="protein sequence ID" value="BAD41164"/>
        <property type="gene ID" value="STH2179"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2179"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_3_1_9"/>
    <dbReference type="OMA" id="ESFSAWH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="418" mass="46038" checksum="37DA9D17E87714E5" modified="2004-10-11" version="1">MEIQPGERVKVTFWHAMGGVAGEAVQRLVDQFNSSQDKVEVEAVYQGTYDDALQKLRAAGSDGPTIMQVYEIGSRFMIDSGMITPMQNFVDADGFSIDDFEPNILGYYTFDGRLYSMPFNTSTPIVYYNKDAFREAGLDPERPPRTFEEFKEYARKLTVTQGGETRYGASIALYGWFFEQFLAVQGAHYVDNDNGRSARATKAIINSPEGERFVTWLKEMVDEGVAINLGRRTADTQAAFSSGQVAMTLDSTAALGNILNGVGDKFEIGTAFLPRPEGAAEGGVIIGGASLWITNTKPLKEQWAAWEFVKWLTTPEVQAEWSIATGYFPVRKAAYDQQILKDWHAQRPQFTTAIEQLRASPLSTVTQGAVIGTFPQARQIVEAAMESVVLGQATAAEALNAAAAEITKGIEDYNRTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG7</accession>
    <name>Q67MG7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196266.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2141"/>
    <dbReference type="EnsemblBacteria" id="BAD41126">
        <property type="protein sequence ID" value="BAD41126"/>
        <property type="gene ID" value="STH2141"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2141"/>
    <dbReference type="eggNOG" id="COG0586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044208_1_1_9"/>
    <dbReference type="OMA" id="FMLALRY"/>
    <dbReference type="OrthoDB" id="1928533at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="22487" checksum="FA0582B0D0B7F50A" modified="2004-10-11" version="1">MDWTTRIMEAFGYPGIVLVVLIENLFPPIPSEIVLPFAGFMTTQGSLTLFGVVLAATVGSVLGAVALYGVGLWFGRDRIYWIVRRYGRWLTVTEADVQRTEDWFERYGMWTVFFCRMVPIMRSLISIPAGLVRMNMGLFVVYTAIGSAVWNLVLVGLGAALGATWDRVSHWVSIYQDVVIAVLLLGAAAFLLYRLLRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS7</accession>
    <name>Q67MS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196157.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2031"/>
    <dbReference type="EnsemblBacteria" id="BAD41016">
        <property type="protein sequence ID" value="BAD41016"/>
        <property type="gene ID" value="STH2031"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2031"/>
    <dbReference type="HOGENOM" id="CLU_2119888_0_0_9"/>
    <dbReference type="OrthoDB" id="2060174at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269903" evidence="1">
        <location>
            <begin position="30"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12307" checksum="B3227A1A0CFDB6FA" modified="2004-10-11" version="1">MRRWGLAALPAAALAAAVALAGCGGKQSAEPKLAVTASQEDGALVVRISTENWQPGKDGHVHIYLNDGPEAMIYGYTYRVPGIEPGRYKIHVELANPRHEHIGVSETIYFDVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU5</accession>
    <name>Q67MU5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FliMN_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2013</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FliN/MopA/SpaO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2013"/>
    <dbReference type="EnsemblBacteria" id="BAD40998">
        <property type="protein sequence ID" value="BAD40998"/>
        <property type="gene ID" value="STH2013"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2013"/>
    <dbReference type="eggNOG" id="COG1886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1219200_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012826">
        <property type="entry name" value="FliN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001172">
        <property type="entry name" value="FliN_T3SS_HrcQb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00956">
        <property type="entry name" value="FLGMOTORFLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02480">
        <property type="entry name" value="fliN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="5" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="8">
        <location>
            <begin position="150"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="227" mass="22472" checksum="6FC5BE02505A1F92" modified="2004-10-11" version="1">MLEEERAALANKLSQSEIDALVASLLAADNAEKQAADGQSGQGSPETAAPAAGQNAEGAPSEFESVQDLGPVTQAELDAAVAASRAAKMKGTAPAAPETAPPLGAPAAPFEAAAAAAAAVPAPAPAAPAGPGPGASKAQLRIPPALRGVLLDIELTLSVELGKARLPLGEILSMGPGSVITLDRLASEPLNVKVNGLPAMTAEVIAIGEKYGIRIVESQLPNYAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY4</accession>
    <name>Q67MY4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196101.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1974"/>
    <dbReference type="EnsemblBacteria" id="BAD40959">
        <property type="protein sequence ID" value="BAD40959"/>
        <property type="gene ID" value="STH1974"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1974"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1539259_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="18338" checksum="3F06221592C019CE" modified="2004-10-11" version="1">MGRRTPPGPLPVTLREVSFRYPGADQDVLDRIAVAIPPGQVVVLVGPNGAGKSTLAALILELYPPTRGSVHVGTGAVGHAVRGAVFQDFARYSLPLRDNVGFGDLSLLHNDDHLRAVLRRTGVALHADLDQWLGAEFGGRELSGASGHGWPSPGGWSGGGDWWSWTSPPPPSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB4</accession>
    <name>Q67NB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Geranylgeranyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1844"/>
    <dbReference type="EnsemblBacteria" id="BAD40829">
        <property type="protein sequence ID" value="BAD40829"/>
        <property type="gene ID" value="STH1844"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1844"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_0_0_9"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="31940" checksum="8E46480E3AC7321C" modified="2004-10-11" version="1">MRGEFQQYMAARRQQVEAALDRYTAGLDAPSRLVEAMRYSLLAGGKRLRPILTIAASELFGLEAADVMPGACALEMIHTYSLIHDDLPCMDDDDLRRGRPTCHKVYGEAMAVLAGDALLTQAFALMAEQAAVPRVGPARAVRAAAELAHAAGAEGMVGGQVEDLAWEGRQADEPQLRRIHSLKTGALFRAAVRMGGILAGAGEEDLDRLGRYATHFGLAFQIWDDVLDVTGDAALTGKGVGRDARLDKSTYVTLHGVEGARERAYQAVRLALEELEPFGGAGWVLRALAESVVDRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ8</accession>
    <name>Q67NJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1760"/>
    <dbReference type="EnsemblBacteria" id="BAD40745">
        <property type="protein sequence ID" value="BAD40745"/>
        <property type="gene ID" value="STH1760"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1760"/>
    <dbReference type="HOGENOM" id="CLU_1634529_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="17228" checksum="640AC44273DBD52E" modified="2004-10-11" version="1">MAVDGTHGRCANVIYSGGCLGMKRQALWGMGAVVLAVLLITGMRPAVLAMDDVVEALERAGLQVAVQETVSWPVLGEGTRLALGADGAHTLVAFIYASQEEQERFEVGEGGYRVTDGNTAALFEWDSPQTVIRHRNVMIIMPADSPALATVRAATLAWGMVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM1</accession>
    <name>Q67NM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1737"/>
    <dbReference type="EnsemblBacteria" id="BAD40722">
        <property type="protein sequence ID" value="BAD40722"/>
        <property type="gene ID" value="STH1737"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1737"/>
    <dbReference type="eggNOG" id="COG2839">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109297_0_1_9"/>
    <dbReference type="OMA" id="KWGQIGA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007403">
        <property type="entry name" value="DUF456"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04306">
        <property type="entry name" value="DUF456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="16449" checksum="680AF5EAD31BECB3" modified="2004-10-11" version="1">MMGWQWIAFAIAVVVMLAGVVGTLIPALPGLPIVFLAMLGYAVVDGFRDITPGFLAVALLVVAATQVAEHYARAWGAKRFGAGRAGAWGAVIGSIVGLFFMPLGLLLGPFLGALLFELVAGRSGSEAVKAGFGGLVGVLGSVVVNVVVALGLTVAFVAKVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR2</accession>
    <name>Q67NR2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Purine nucleoside phosphorylase</fullName>
            <shortName evidence="1">PNP</shortName>
            <ecNumber evidence="1">2.4.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Purine nucleoside phosphorylase involved in purine salvage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer. Dimer of a homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195824.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1696"/>
    <dbReference type="EnsemblBacteria" id="BAD40681">
        <property type="protein sequence ID" value="BAD40681"/>
        <property type="gene ID" value="STH1696"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1696"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_9"/>
    <dbReference type="OMA" id="ADPFCPE"/>
    <dbReference type="OrthoDB" id="1364220at2"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="2">
        <location>
            <begin position="7"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="1">
        <location>
            <begin position="54"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="1">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="264" mass="28399" checksum="0CEFEC23E4E09180" modified="2004-10-11" version="1">MTQKKVRLAIIGGTGVYDPRILENIREEVITTPYGQAKVRIGTYAGEEVAFMARHGVDHSVPPHLINYRANIYALKMLGVERVIATTAVGSCNRNMKPGDFVVVDQFLDFTKGRVSTFFEGGEAGVVHTDVTEPYCPELRKVLIAAAQEVGVHAHPTGTYVCTEGPRFETAAEIRMYGQLGGDLVGMTNVPECVLAREAGICYATVSMVTNFGAGISPNPLTHEEVLEVMAANGENLKKLLFAALRQIPAGRGCACGSNTALKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU3</accession>
    <name>RECA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recA</name>
        <name type="ordered locus">STH1665</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195794.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NU3"/>
    <dbReference type="SMR" id="Q67NU3"/>
    <dbReference type="STRING" id="292459.STH1665"/>
    <dbReference type="EnsemblBacteria" id="BAD40650">
        <property type="protein sequence ID" value="BAD40650"/>
        <property type="gene ID" value="STH1665"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1665"/>
    <dbReference type="eggNOG" id="COG0468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040469_1_2_9"/>
    <dbReference type="OMA" id="EGARHMS"/>
    <dbReference type="OrthoDB" id="1080436at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000122872" description="Protein RecA">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <sequence length="350" mass="37503" checksum="A8D365360C97E019" modified="2004-10-11" version="1">MALDPAKQKAIDMALAQIEKQFGKGSIMRLGEQMSRLNVEVIPTGSVALDVALGVGGLPRGRITEIYGPESSGKTTVALHVIAEAQKMGGVAAFVDAEHALDPVYAQALGVDIDNLLVSQPDTGEQALEITETLVRSGAVDVVVVDSVAALVPKAEIEGEMGDSFVGLQARLMSQALRKLTGAISKSKAVVIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLEVRKTETLKSGQEVVGSRTRVKVVKNKVAPPFKQAEFDILYGQGISKEGSLIDIGTELNPPVIVKSGAWYSYGDLRIGQGKDNARDWLRQHPEVAAEIEQKIKERLNVASLPVKYADEEAGDADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P27</accession>
    <name>Q67P27_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195710.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1581"/>
    <dbReference type="EnsemblBacteria" id="BAD40566">
        <property type="protein sequence ID" value="BAD40566"/>
        <property type="gene ID" value="STH1581"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1581"/>
    <dbReference type="eggNOG" id="COG1376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782861_0_0_9"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YkuD" evidence="3">
        <location>
            <begin position="210"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="354" mass="38304" checksum="C905E68DD0A2CCE0" modified="2004-10-11" version="1">MSHLHEPDAHYSARRAWRRREARRRRYARRRALALLTLIGVLVGLGFGVRWAVMRIRHALAPPSSPDVTEPVEQAPAALPFAPERILEAPDLTGDGEPERVAVSAADDGRLQVALVDGWGPDAALLGQPISMPEGPVEIMDLPRAQGLLVWRGALPRLGEPVEVSVGGEPALEARGGEPFFRAWALDAEHGLVPADYYAALAPVAPPEPTVIVVDKGLNVLWYYEDGELVQTARVSTGRHVAGPAPSPDNWTENLLTPTGRFTVTLMVPGMPYYKEGIDALDPANPLGTRWIGFTVFEGDGGSLWAIHGTNAPEALGRWNSEGSIVMSNGEVEQLYERVELGTPVIITNSLEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P32</accession>
    <name>Q67P32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195705.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1576"/>
    <dbReference type="EnsemblBacteria" id="BAD40561">
        <property type="protein sequence ID" value="BAD40561"/>
        <property type="gene ID" value="STH1576"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1576"/>
    <dbReference type="HOGENOM" id="CLU_959395_0_0_9"/>
    <dbReference type="OrthoDB" id="260889at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="PFTB_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="261" mass="27373" checksum="F4251A52FCD58C25" modified="2004-10-11" version="1">MALPQIDVARAKAFVMAHGSAVDVTRLEGILGRDQPDRSVVRTLEERQNPDGGFPVDAPGASSVVATCTLIQWLRDIPPLAGAPMAGRAVSFVRRSQQVDGSWIDPGTTGAAAQAYMTARAVYTLLVAEPDHPDPIARGARWLRGALEDGTDQADTETLILAAAIWSRPPGGGPGDALADAAYASLRDRALSPRELALWLTTFVDLGLAARHLGLAVGLLDRLAGLQQPDGGWPAEDGGRVDATLGALRAFRGFGLIGPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P54</accession>
    <name>Q67P54_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1554</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1554"/>
    <dbReference type="EnsemblBacteria" id="BAD40539">
        <property type="protein sequence ID" value="BAD40539"/>
        <property type="gene ID" value="STH1554"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1554"/>
    <dbReference type="HOGENOM" id="CLU_2756364_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="70" mass="8244" checksum="DEB3365BA0154884" modified="2004-10-11" version="1">MRSTLHIVSACDYLRIRQALQPVLSRDQFYCSRDAQREQDDPKPALCVHLMQREDPAPFPTLHGHMRIRQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P67</accession>
    <name>CHEB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="1">3.1.1.61</ecNumber>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheB</name>
        <name type="ordered locus">STH1541</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="1"/>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195671.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P67"/>
    <dbReference type="SMR" id="Q67P67"/>
    <dbReference type="STRING" id="292459.STH1541"/>
    <dbReference type="EnsemblBacteria" id="BAD40526">
        <property type="protein sequence ID" value="BAD40526"/>
        <property type="gene ID" value="STH1541"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1541"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_51_0_9"/>
    <dbReference type="OMA" id="MLEMHRA"/>
    <dbReference type="OrthoDB" id="1655418at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018277">
        <property type="term" value="P:protein deamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006482">
        <property type="term" value="P:protein demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225487" description="Protein-glutamate methylesterase/protein-glutamine glutaminase">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="10"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="1">
        <location>
            <begin position="173"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <sequence length="362" mass="38488" checksum="A9B6282EB92B56ED" modified="2004-10-11" version="1">MSDRVQRPIRVLVVDDSAFMRKVLTELLESDPLITVVGTARDGQDGLEKVLQLQPDVVTLDIEMPRLDGYGTLREIMARRPTPVVMVSSHTREGAEATIRALALGAVDFVAKPSGSISLNMHVARDELVAKVKAAAAATPRVRTAAVELAPVSPREKMQALAGLRRQTGFGDRLPRRLVLIGCSTGGPGALHQIIPRLPADLPAGVLVVQHMPPGFTRSLAQRLDELSAISVREARAGDPVRAGQVLVAPGGYHMLVDAEGRIALDQGPPVHGVRPAVDVTFESVLPVWKERLVGVILTGMGYDGAKGMAQLRKAGGWTIAEDASTCVVYGMPRVVVELGAAREVLPVHSIADAITRAVGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI7</accession>
    <name>Q67PI7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative aerotaxis receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195551.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1421"/>
    <dbReference type="EnsemblBacteria" id="BAD40406">
        <property type="protein sequence ID" value="BAD40406"/>
        <property type="gene ID" value="STH1421"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1421"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_26_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="157"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="210"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="265"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="346"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="403"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="665" mass="70722" checksum="92F61B69758F12DB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR4</accession>
    <name>FMT_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fmt</name>
        <name type="ordered locus">STH1344</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PR4"/>
    <dbReference type="SMR" id="Q67PR4"/>
    <dbReference type="STRING" id="292459.STH1344"/>
    <dbReference type="PRIDE" id="Q67PR4"/>
    <dbReference type="EnsemblBacteria" id="BAD40329">
        <property type="protein sequence ID" value="BAD40329"/>
        <property type="gene ID" value="STH1344"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1344"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_1_1_9"/>
    <dbReference type="OMA" id="CCPVVAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000083066" description="Methionyl-tRNA formyltransferase">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="326" mass="34785" checksum="7037684E357120BC" modified="2004-10-11" version="1">MLKILFMGTPEFAAVSLRALLEAGYPVVGVVTQPDKPAGRGGKLRPSPVKEVALAHGLPVFQPRRLRRPEVVAQLKELGSDLTVVVAYGQILSREALEISPLGSINVHASLLPRWRGAAPIQRAIMAGDVETGVCTMWMDEGMDTGDVCLTARVPIGPDTTGGELHDELARVGAELLLETVRRVEAGDAPRIPQPAEGVTYAAKLEPADEVIDWARPAEELYNQIRALNPWPGAYTNGPRGRLKIWRASVVPNPEPGAEPGTVVALVRREGFTVAAGDGALLVREVQPHGKARMDAQSFVNGGGVQVGTRFSPPEAPQREPAPGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV0</accession>
    <name>Q67PV0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1308</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195439.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1308"/>
    <dbReference type="EnsemblBacteria" id="BAD40293">
        <property type="protein sequence ID" value="BAD40293"/>
        <property type="gene ID" value="STH1308"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1308"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026842_1_0_9"/>
    <dbReference type="OMA" id="APHANID"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="12"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="500" mass="57115" checksum="D0A1C18A62E05101" modified="2004-10-11" version="1">MKQQIKKRKRVKLTDQQRRCVNWPLDQDLLIRGIPGSGKTTVLLERAYRIETAGGTPSPRILVLTFSSLLTAYVRQLALEEGNGLIEAQTFHGWGLQLLQDVGYAPRGGVMKEQDREAQIKFARNIIRKYRQDLKPPSFGANRKPPVDPRRAKVMELEFLKEEFSWIKGLGKNRDRYISEPRTGRGNTVRVDRENREYIWAVYEKYEELNRNQKKWDFDDVALKLLEAKRLGLITPDRCPQYVLVDEAQDLTVMQLRAIRELTTVSLTIAADKGQSIYQRNFSWTELGIDIRGRSRSLQQTFRSTRQIIRLARSLQRHDPLVITGSDEYVPAADPDVNGPVPILYVAPDLETQIQQVVQWALRLLATPGLEEDTIGIIVPTASLRERYAQALEDAGVPYLILGRDHSYDAVSPGIKLVTFHSAKGLEFDHVAVTSLREGSIPSSLSRDLSAEDREEFIATERRKVYVAMTRARLTLALFACRPVSQFVIEMDPTLYRRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q13</accession>
    <name>Q67Q13_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fructose-bisphosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195376.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1245"/>
    <dbReference type="EnsemblBacteria" id="BAD40230">
        <property type="protein sequence ID" value="BAD40230"/>
        <property type="gene ID" value="STH1245"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1245"/>
    <dbReference type="eggNOG" id="COG0191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040088_0_1_9"/>
    <dbReference type="OMA" id="AMYAIPE"/>
    <dbReference type="OrthoDB" id="827430at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011289">
        <property type="entry name" value="Fruc_bis_ald_class-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01859">
        <property type="entry name" value="fruc_bis_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="209"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="230"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="30213" checksum="BE4A7CF7C63C6652" modified="2004-10-11" version="1">MALVTLKEALAQAEAGGYAVPAINVHSLEVLPAVLQVAAEERSPVILQFTEGTLKFCGYDNVRVLAGHLARVTPVPVVLHQDHGASYEVAVRAIQAGFSSVMIDASHLPLEENIAATRKVVELAHACGVSVEAELGRVPGQEEEVVVSEAEATYTDPAEAARFVAETGVDALAVAVGTVHGFYKWEPKLAHDRIAAIREAVRIPLVLHGGSGVPDAEVQRAIQLGIRKVNVSTEGKYAWAQALRQSLADQPGEFDPRPLLTPALKAVQEVVRAKIRMVGSHGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q87</accession>
    <name>Q67Q87_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195302.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1171"/>
    <dbReference type="EnsemblBacteria" id="BAD40156">
        <property type="protein sequence ID" value="BAD40156"/>
        <property type="gene ID" value="STH1171"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1171"/>
    <dbReference type="eggNOG" id="COG2718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049702_2_0_9"/>
    <dbReference type="OMA" id="RICFLAH"/>
    <dbReference type="OrthoDB" id="481720at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014230">
        <property type="entry name" value="Spore_YhbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006698">
        <property type="entry name" value="UPF0229"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30510">
        <property type="entry name" value="PTHR30510"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04285">
        <property type="entry name" value="DUF444"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02877">
        <property type="entry name" value="spore_yhbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="45289" checksum="83AE3C7373A88B45" modified="2004-10-11" version="1">MADLPFVISREDWSLHRQGQMDQERHQARIREAIRANLADIVSDEAIIASDGRKVVRLPIRVLREYRFRLDWQKQPRVGEADGPVRPGEPVGRPGRAAEAAGGSGAGDEAGEDWFETDVPLEELEELLFAELSLPHLEPKQEPHLTVLHHEWRDVRRQGLYANIDKKRTLLEAMKRNRLAGRPPLAGIRREDLRFRTWDEAEIPGASAVLIIMMDTSGSMGTGEKYIARSLCHWMVRFLRTRYERVKLHFVAHTTEAKEMDEESFFTRGESGGTRCSSAYEYALQLIDRRYPPDRHNLYAFHFSDGDNLISDNPRAVALLRKLLERCALVGYGQIETQPQYLSMPYYQPNTLLTLFREEIDHPRFVTALIRDRSEIYAALRAFFPRPGAGERGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q88</accession>
    <name>Q67Q88_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoVR</name>
        <name evidence="1" type="ordered locus">STH1170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195301.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1170"/>
    <dbReference type="EnsemblBacteria" id="BAD40155">
        <property type="protein sequence ID" value="BAD40155"/>
        <property type="gene ID" value="STH1170"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1170"/>
    <dbReference type="eggNOG" id="COG2719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010179_1_0_9"/>
    <dbReference type="OMA" id="EFARMHA"/>
    <dbReference type="OrthoDB" id="507759at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007390">
        <property type="entry name" value="Spore_V_R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30029">
        <property type="entry name" value="PTHR30029"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04293">
        <property type="entry name" value="SpoVR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="408" mass="47205" checksum="4B3CB5E4ACCD4177" modified="2004-10-11" version="1">MACDLDRLGEAVEAVVRRARAAGLDFFPLRFELCPPDVLYTFGAYGMPTRFSHWSFGKAYHLLKSQAKLGLTKIYELVINSDPCHAFLLESNSLLENTLVAAHVLAHADFFKHNWRFQSTNRRMLHSMSAFAERVRRYEERLGRGPVERFLDAALAVAEQIDPFPGPRPAPQRDLPLFLLERAPELADWERDILASLREEALYFRPQMETKILNEGWATFWHVRLLRELDLPPAQAVEFARMHAAVIAPHPHQLNPYRLGYHLLESIEARYGRERLFEVRTLETDVSLVRNYLTPELCQALNLHVYAQQNGQWQVAATDWTAVRDLLLRQLENCGLPYIYAEDDNYQGRGELYLRHGYDGVELDVTHLTHALRHVHHIWRRPVHLETVQGGRNTVYTCHGEEVTSRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q89</accession>
    <name>Q67Q89_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1169"/>
    <dbReference type="EnsemblBacteria" id="BAD40154">
        <property type="protein sequence ID" value="BAD40154"/>
        <property type="gene ID" value="STH1169"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1169"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1081533_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="41"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="28412" checksum="FDC9599B68907E3C" modified="2004-10-11" version="1">MGMSDFCRRKRSGVQARTGGAPMSLMVREQAFAAVPQEVVVDRLRPLLSPRELEVAQLALSGLPTAEVARRLFVTVNTVKTHIRHILRKTGTESRAELVRRLLMEEELAAGKRDPVTGFLPRTAFRRRALDLMRKHRGQGVSLIWLEAQGRQRLSVVERVAADLAVAEALSEAVRAPDLAFRWSEDQFLGLLPATDREVARTVTMRLGLWLGEWASVSGCDLLFSLACSSSLEGLTSPEALMEAAVRRSGEVLEDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q91</accession>
    <name>Q67Q91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195298.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1167"/>
    <dbReference type="EnsemblBacteria" id="BAD40152">
        <property type="protein sequence ID" value="BAD40152"/>
        <property type="gene ID" value="STH1167"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1167"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_36_1_9"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17427" checksum="63C1C8E4EA0837CB" modified="2004-10-11" version="1">MAIQYRAATRADLNALLPLVEAFAREQQNQLGLYSLAEGFMQFAKTGLAQALEHPAAVVMIAEDEGRGEGPVGYCVGSLQEPPALFRPELYTYISDLYVLPEYRRRGIGTGLVERVRGWGYLKGAFSVSMIVPTGSAAVGLGERVGMRPIQVLMYASGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC1</accession>
    <name>Q67QC1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Cysteine synthase</fullName>
            <ecNumber evidence="5 11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH1137</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5 11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195268.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1137"/>
    <dbReference type="EnsemblBacteria" id="BAD40122">
        <property type="protein sequence ID" value="BAD40122"/>
        <property type="gene ID" value="STH1137"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1137"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_9"/>
    <dbReference type="OMA" id="RTPVFKF"/>
    <dbReference type="OrthoDB" id="1033353at2"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="9"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="179"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="32751" checksum="51DA308BB1F0214D" modified="2004-10-11" version="1">MARIYDSIADTIGRTPLVRLNRVTEGAVATVLAKVEAFNPASSVKDRIGYAMIKDAEEKGILKPGMTIVEPTSGNTGIALAFVAAAKGYRCILTMPDTMSLERRKLLKAFGAELVLTPGAEGMKGAIAKAKEILENTPGAWMPQQFENPANPEIHRKTTAEEIWQDTDGQVDIVVGGVGTGGTLTGVGEVIKARKPDFKVVAVEPAESPVISGGKPGPHKIQGIGAGFIPKNLNTEVVDEVITVASEDAIATARRMAREEGLLVGISSGAAAFAALQVARRPENAGKTIVVVLPDTGERYLSSALWADIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD1</accession>
    <name>Q67QD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195258.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1127"/>
    <dbReference type="EnsemblBacteria" id="BAD40112">
        <property type="protein sequence ID" value="BAD40112"/>
        <property type="gene ID" value="STH1127"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1127"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_3_9"/>
    <dbReference type="OMA" id="VWCFGET"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="ThiF" evidence="2">
        <location>
            <begin position="11"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="244" mass="24391" checksum="AFE17FF50E28C9D7" modified="2004-10-11" version="1">MTLTEQEILRYSRHIILPEVGGRGQQRIKSASVLVAGLGAAGSAAALYLAAAGVGRLTLWDPAPLAEADLARAIAHDRAHLGLSRAASARGKVTAINPDADVLASAEPDALPGLVNGHQVVLATAGDWEAVQAAALRTGAAAVFAGVRGAAGAVFAHRPGEPCLGCVPPEAREAAGLQPEEGGGCAVAAAAGVVGIAAATEALKLILGIGAPLTGRLWAYDGWAAGSREVPVTRRADCALCGRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE3</accession>
    <name>MUTS2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease MutS2</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutS2</name>
        <name type="ordered locus">STH1115</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195247.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QE3"/>
    <dbReference type="SMR" id="Q67QE3"/>
    <dbReference type="STRING" id="292459.STH1115"/>
    <dbReference type="PRIDE" id="Q67QE3"/>
    <dbReference type="EnsemblBacteria" id="BAD40100">
        <property type="protein sequence ID" value="BAD40100"/>
        <property type="gene ID" value="STH1115"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1115"/>
    <dbReference type="eggNOG" id="COG1193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011252_2_1_9"/>
    <dbReference type="OMA" id="IHAIIND"/>
    <dbReference type="OrthoDB" id="256392at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03280">
        <property type="entry name" value="ABC_MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00092">
        <property type="entry name" value="MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005747">
        <property type="entry name" value="MutS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF14">
        <property type="entry name" value="PTHR11361:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005814">
        <property type="entry name" value="MutS_YshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01069">
        <property type="entry name" value="mutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000093409" description="Endonuclease MutS2">
        <location>
            <begin position="1"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Smr" evidence="1">
        <location>
            <begin position="717"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="329"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="793" mass="87439" checksum="457DB102EAAAFCC3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF4</accession>
    <name>Q67QF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1104"/>
    <dbReference type="EnsemblBacteria" id="BAD40089">
        <property type="protein sequence ID" value="BAD40089"/>
        <property type="gene ID" value="STH1104"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1104"/>
    <dbReference type="HOGENOM" id="CLU_2792492_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7473" checksum="0D422D163B0459C3" modified="2004-10-11" version="1">MPFSAVMCCSLAVAFPLWYTYSHQVHGTRVEGGNGGMSNSAAAEVFWTIFSNTGSVIAYLLYKRFTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL5</accession>
    <name>Q67QL5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1043"/>
    <dbReference type="EnsemblBacteria" id="BAD40028">
        <property type="protein sequence ID" value="BAD40028"/>
        <property type="gene ID" value="STH1043"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1043"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2060230_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269315" evidence="1">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="74"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="119" mass="13036" checksum="79DC4D702F63E9A2" modified="2004-10-11" version="1">MRRFVAGMLAVLMLAAVGCSGRSSSGSQEMVIRMNIGTNPPSLDPRVTTGLPESHVEIALFEGLMRLDEKGNAIPGAAERYEVNDDATVFTFYLRDGLKWSNGDPLTAEDFVWTWKSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS4</accession>
    <name>Q67QS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195116.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39969">
        <property type="protein sequence ID" value="BAD39969"/>
        <property type="gene ID" value="STH984"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH984"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="WIAHASH"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49959" checksum="8E6A3AD284F4320B" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRCLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGLACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS8</accession>
    <name>Q67QS8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195112.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH980"/>
    <dbReference type="EnsemblBacteria" id="BAD39965">
        <property type="protein sequence ID" value="BAD39965"/>
        <property type="gene ID" value="STH980"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH980"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514737_0_0_9"/>
    <dbReference type="OrthoDB" id="991474at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00118">
        <property type="entry name" value="BLACTAMASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269501" evidence="2">
        <location>
            <begin position="35"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase2" evidence="3">
        <location>
            <begin position="176"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="529" mass="53155" checksum="572BCC6C30B1989C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY3</accession>
    <name>Q67QY3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH925"/>
    <dbReference type="EnsemblBacteria" id="BAD39910">
        <property type="protein sequence ID" value="BAD39910"/>
        <property type="gene ID" value="STH925"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH925"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055284_1_0_9"/>
    <dbReference type="OMA" id="CASGFAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="46"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="30891" checksum="461D8622D2259482" modified="2004-10-11" version="1">MKVTTLSRQARCRQPRRRQIQYLHCASLPYCCCGRELAEGMTDRALVMGGGGVLGIAWMVGLTAAWKEAGVDLGSADLVVGTSAGSVVGTLIRYGVDPAQLTHLVAAGMGLPPAAVAKVATGAGLTEDEWVGLFQAPLGDVPWPEKPLRISAVDAETGEPVMWTNASGIPLARALASSCAVPGLLPTVLINGRRYVDGGAWSGTHADQAAGFRRVLIVAPMGGPKYPRGHEMLQKERAALEADGSRVLTVLPDPATLQVFGDNLMDPARIFDALENGRRQGAEAALQVRSFWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY8</accession>
    <name>Q67QY8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH920</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195052.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH920"/>
    <dbReference type="EnsemblBacteria" id="BAD39905">
        <property type="protein sequence ID" value="BAD39905"/>
        <property type="gene ID" value="STH920"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH920"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_6_9"/>
    <dbReference type="OMA" id="NCERLQQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017205">
        <property type="entry name" value="Sig_transdc_His_kinase_ChrS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037434">
        <property type="entry name" value="STHK_ChrS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269503" description="Histidine kinase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="70"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="248"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="38264" checksum="E90F4F7EEF0F36D9" modified="2004-10-11" version="1">MRLLSYLHSRTSLFTKVLVANAAIAVIGATCAVLIATHFWNSQEHAPIWEFGLIAVGLVVSLGVNYALLRVAFRPLFTLHATLDRVRRGDFSARVPMVPGDPDIARVSEMANLMLDRLAEHRQAVAAQILRAQEEERKRIARELHDETAQSLTSIVVNLEAIERQAVGSGVDEALCERLHLTKEVAQRTLDETRRLMMDLRPSVLDDLGLVPALRWFINHRVLPAGLEADFQVSGLTERLPEELETALFRIMQEAITNVVKHAKAKTVLVRLHREHGEITGLVVDDGQGFHVVHTVGQPVRDRGLGLFGMQERAALVGGKVVVESAPGRGTTVRVTVPDRRGGDVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-02-07" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ1</accession>
    <name>NAPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nitrate reductase</fullName>
            <ecNumber evidence="1">1.9.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">napA</name>
        <name type="ordered locus">STH917</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of the nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 Fe(II)-[cytochrome] + 2 H(+) + nitrate = 2 Fe(III)-[cytochrome] + H2O + nitrite</text>
            <dbReference type="Rhea" id="RHEA:12909"/>
            <dbReference type="Rhea" id="RHEA-COMP:11777"/>
            <dbReference type="Rhea" id="RHEA-COMP:11778"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.9.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
        <text evidence="1">Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the nitrate reductase NapAB complex composed of NapA and NapB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <text evidence="1">Membrane-associated.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.9.6.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195049.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QZ1"/>
    <dbReference type="SMR" id="Q67QZ1"/>
    <dbReference type="STRING" id="292459.STH917"/>
    <dbReference type="PRIDE" id="Q67QZ1"/>
    <dbReference type="EnsemblBacteria" id="BAD39902">
        <property type="protein sequence ID" value="BAD39902"/>
        <property type="gene ID" value="STH917"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH917"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_9"/>
    <dbReference type="OMA" id="NLAWCHP"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050140">
        <property type="term" value="F:nitrate reductase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02791">
        <property type="entry name" value="MopB_CT_Nitrate-R-NapA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01630">
        <property type="entry name" value="Nitrate_reduct_NapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041957">
        <property type="entry name" value="CT_Nitrate-R-NapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010051">
        <property type="entry name" value="Periplasm_NO3_reductase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0500">Molybdenum</keyword>
    <keyword id="KW-0534">Nitrate assimilation</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" description="Tat-type signal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000046008" description="Nitrate reductase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="256"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="745"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01630"/>
        </source>
    </evidence>
    <sequence length="754" mass="83580" checksum="2754BFA565A43AF0" modified="2004-10-11" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R59</accession>
    <name>FPG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Formamidopyrimidine-DNA glycosylase</fullName>
            <shortName evidence="2">Fapy-DNA glycosylase</shortName>
            <ecNumber evidence="2">3.2.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase MutM</fullName>
            <shortName evidence="2">AP lyase MutM</shortName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mutM</name>
        <name evidence="2" type="synonym">fpg</name>
        <name type="ordered locus">STH849</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.23" evidence="2"/>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194981.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67R59"/>
    <dbReference type="SMR" id="Q67R59"/>
    <dbReference type="STRING" id="292459.STH849"/>
    <dbReference type="EnsemblBacteria" id="BAD39834">
        <property type="protein sequence ID" value="BAD39834"/>
        <property type="gene ID" value="STH849"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH849"/>
    <dbReference type="eggNOG" id="COG0266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038423_1_2_9"/>
    <dbReference type="OMA" id="GVHLRMT"/>
    <dbReference type="OrthoDB" id="1162346at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00103">
        <property type="entry name" value="Fapy_DNA_glycosyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015887">
        <property type="entry name" value="DNA_glyclase_Znf_dom_DNA_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020629">
        <property type="entry name" value="Formamido-pyr_DNA_Glyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00577">
        <property type="entry name" value="fpg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01242">
        <property type="entry name" value="ZF_FPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000228477" description="Formamidopyrimidine-DNA glycosylase">
        <location>
            <begin position="2"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FPG-type" evidence="2">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="3"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for beta-elimination activity" evidence="2">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for delta-elimination activity" evidence="2">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="305" mass="33684" checksum="B47EDEC0828DB164" modified="2007-01-23" version="3">MPELPEVETVRRTLCPRVVGRRIRRVEILTPRQIYHPDPATFAADLEGAVFDDIERRGKYLLFRLGPRILVAHLRMSGHLYVCGPEAPRPRHLHVVFHLDDGGELRYADQRKFGGFHLLGPGGEGMPPGLANLGPEPLSPEFTPQVLAERLAGRHTSIKAALLNQALVAGLGNIYADEALFCARIHPAREAGSLTPAEVERLHGCIRRVLLRAVERRGTTFSLYRDGEGNEGDMYDELQVFDRAGEPCPVCGTPIRKVAVAQRGTHFCPRCQPVPEGVALSPRRARPGRRGNSVRVAAEPPGTYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R89</accession>
    <name>Q67R89_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chorismate dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.151</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Menaquinone biosynthetic enzyme MqnA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mqnA</name>
        <name evidence="4" type="ordered locus">STH819</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the dehydration of chorismate into 3-[(1-carboxyvinyl)oxy]benzoate, a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>chorismate = 3-[(1-carboxyvinyl)-oxy]benzoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:40051"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:76981"/>
            <dbReference type="EC" id="4.2.1.151"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MqnA/MqnD family. MqnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.151" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH819"/>
    <dbReference type="EnsemblBacteria" id="BAD39804">
        <property type="protein sequence ID" value="BAD39804"/>
        <property type="gene ID" value="STH819"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH819"/>
    <dbReference type="eggNOG" id="COG1427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059898_0_0_9"/>
    <dbReference type="OMA" id="WGLARTG"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00995">
        <property type="entry name" value="MqnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003773">
        <property type="entry name" value="Menaquinone_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030868">
        <property type="entry name" value="MqnA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37690">
        <property type="entry name" value="PTHR37690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02621">
        <property type="entry name" value="VitK2_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="32434" checksum="43F1AB572C9F903F" modified="2004-10-11" version="1">MPAGRLPGRKAATAAPEAPPIRLGYIDYLNCLPVYFGIEQGAVELDVTVQKGPPSALNRAFREGRLDVTPVSSIEYARHAADCVIIPGLSISADGRVASILLFHRRPLEELDGRPVALTASSATSVVLTRIILELRYGVRPAYRVAPPDLDAMLAEHDAALLIGDDALLAAQAYPEIPHVDLGAEWKAFTGHPMVYALWVARRELAEADPAALRRVVRVFHASQEYAWDRRPEMVAEAVARRGLPPDVVDDYFDLIRHEFGPRYRRGLVAFYEYARRLGELDAVPPLRVWGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R91</accession>
    <name>Q67R91_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminodeoxyfutalosine synthase</fullName>
            <shortName evidence="6">AFL synthase</shortName>
            <shortName evidence="6">Aminofutalosine synthase</shortName>
            <ecNumber evidence="6">2.5.1.120</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Menaquinone biosynthetic enzyme MqnE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mqnE</name>
        <name evidence="8" type="ordered locus">STH817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Radical SAM enzyme that catalyzes the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoate, leading to aminodeoxyfutalosine (AFL), a key intermediate in the formation of menaquinone (MK, vitamin K2) from chorismate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3-[(1-carboxyvinyl)-oxy]benzoate + H2O + S-adenosyl-L-methionine = 6-amino-6-deoxyfutalosine + H(+) + hydrogencarbonate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:33075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64286"/>
            <dbReference type="ChEBI" id="CHEBI:76981"/>
            <dbReference type="EC" id="2.5.1.120"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. MqnE family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.120" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194950.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH817"/>
    <dbReference type="EnsemblBacteria" id="BAD39802">
        <property type="protein sequence ID" value="BAD39802"/>
        <property type="gene ID" value="STH817"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH817"/>
    <dbReference type="eggNOG" id="COG1060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_0_0_9"/>
    <dbReference type="OMA" id="HIKAYWA"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102573">
        <property type="term" value="F:aminodeoxyfutalosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00993">
        <property type="entry name" value="MqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022432">
        <property type="entry name" value="MqnE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03700">
        <property type="entry name" value="mena_SCO4494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="61"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="451" mass="49740" checksum="9FFEF0BBDDAC75F9" modified="2004-10-11" version="1">MGVSTLLERTLDARLAPIYRKVVAGERLSFDDGVTLMTHPNLFAVGQLANLARRLRVGDQVYFNNNAHINYSNVCAIHCKFCAFGQSRRSPRAFTLSIDEIVAKARAAVAQGATELHMVGGLHPDLPWEWYPAMLRAVKAACPDVHLKCFTAIELIHFTRISGLSLEQVLLELKEAGLGSLPGGGAEIFAPRVREAICKPKETAEEWLHVHDTAHRLGLKSNATMLYGTIETPEERVDHLIRLREQQDRSGGFQAFIPLRFHPENTGLAHLPGPTGYDDLRVIAVGRLMLDNFPHIKSYWIASSPAITQVALHFGANDIDGTVREEKIYHMAGARTAQYTELQQLLRMIWEAGRIPAERNTVYEVIRAYPPEPDPQLVADGAIHGYTAENVHLSAAERAIAEGRFHPGGRRARPVPVQFLEDLEQWAGGEGNGAAVDGSRHAGECAAEGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ2</accession>
    <name>Q67RJ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194849.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39701">
        <property type="protein sequence ID" value="BAD39701"/>
        <property type="gene ID" value="STH716"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH716"/>
    <dbReference type="HOGENOM" id="CLU_698143_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="43910" checksum="7B0B02F1D96E6219" modified="2004-10-11" version="1">MLQKLQLVAREGSLSVTDVYLGHIKRDEDRKLLAHADAYRWSDLDPQLRAILTQHLYQAIEGAQGEVNFPCMYTGEVLPVDKVASAEYDRLHNRPSVEVLEAIRDMEEVHPQSLVDLGNVLAYKVMAAGAIKNHLVAILRFRAVPELGATPLRFSFATVLDLEDREESLFDEHTGSFRTQVLNNVIKRGSVSRAVFFPCLDAEGREIADLMVYAGSGAAAWFKALEATRRLSPRREGQALLRMITEQNATGEVPHDLFRRMGADLLDEAADGLSAESVVRSMEKAVGHGVDRLGFQARWESAFGDLSYRPAYNSLFGGAEKPTRMKMRAGSIEITLTPADLESFRQVTVGDRTFILFAVPERARVVVGKDLDLKIKPVGLADIQRWMTGEEDPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK2</accession>
    <name>Q67RK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH706</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194839.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH706"/>
    <dbReference type="EnsemblBacteria" id="BAD39691">
        <property type="protein sequence ID" value="BAD39691"/>
        <property type="gene ID" value="STH706"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH706"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_2_1_9"/>
    <dbReference type="OMA" id="LLMTRYA"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="355" mass="38846" checksum="057AC09F5A5AF8ED" modified="2004-10-11" version="1">MRYPFALECGVYITTYEQDHALHQTPLAVARGWVLVALLYAIPWFLPNYYVSILNMVAIYTIAALGLNLLTGVTGQISVGHGAFTGMGAFTVGYLMKQGLPFWVAMPLAGLTAAAVGALFGLPSARLKGLYLAIASLAAQVILTFFFTRADWFTGGIHSMSVRRPSFFGHTLSGERSYFYLALTVALLLGVFAQNLLRTRIGRAFMAVRDRDLAAEVMGIDLFRTKVTAFALSAFYAGIAGAIWAPYVMIISPEQFEIHLSIELLAMIIIGGMGSVMGSVYGAAFMTVMPIVVRELAMLLEPVFPSVGTKLLLIRDAAFGAAIVLFLIFEPEGLAKLWRNIKDYFRLWPFGYSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN7</accession>
    <name>Q67RN7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39656">
        <property type="protein sequence ID" value="BAD39656"/>
        <property type="gene ID" value="STH671"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH671"/>
    <dbReference type="HOGENOM" id="CLU_1331359_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="22301" checksum="47DA1371E390C115" modified="2004-10-11" version="1">MPPRPYTPWQRNFKVSRMSHLNWSTRAKECVARQAASSKPWSFCRPPSGSVVIETPVGSDVRVRAIKVALEEAARAAGIRALSVSDSGDFFADPLRYGMVGALVYALASLGLFGVAMPIIAMRQQGILRLMRTTPVTRLTFVLAQVPARLILGMALTLCALLAAWALWDVTLPQLAAALGTSVLGFWMSAAFGYLVGGLGGRHGWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP1</accession>
    <name>Q67RP1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194800.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH667"/>
    <dbReference type="EnsemblBacteria" id="BAD39652">
        <property type="protein sequence ID" value="BAD39652"/>
        <property type="gene ID" value="STH667"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH667"/>
    <dbReference type="eggNOG" id="COG1857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044824_1_0_9"/>
    <dbReference type="OMA" id="PTGKQNT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010148">
        <property type="entry name" value="CRISPR-assoc_prot_CT1975"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09344">
        <property type="entry name" value="Cas_CT1975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01869">
        <property type="entry name" value="casC_Cse4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="379" mass="41016" checksum="06E9B7E014BEB2E0" modified="2004-10-11" version="1">MFVEMHLLQNFALSNLNRDDTGAPKSCVFGGTRRARISSQCLKRAVRTYVREQALVPSELLSYRTKWLQRELANRLAAGGVEAEQAGQVAARALELLEFRLKNGRTEYLLMVGEREIARIADLCREHAAALQGGDGGRKSKKEGDNLAGLFLKALDGGDAVDIALFGRMIATHPEKNVDAAVQMAHAFSTNAIANEFDFYSAVDDLQQQDDDEGAGAGMLGTVLYNSSCYYRYANVDLRQLLTNLGGDPDRALTAVRAFLLGMVHAVPTGKRTNSAPQNPPALIMAVVREHGLWSLANAFVVPVSGARGNLMELSAKEMLAHWNQLSELYGQEGVHYAGLATYLSSDAIGASNAVGIAVEKRLADLVDRVLAEVQPALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV2</accession>
    <name>Q67RV2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glucosamine-6-phosphate deaminase</fullName>
            <ecNumber evidence="2">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GlcN6P deaminase</fullName>
            <shortName evidence="2">GNPDA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nagB</name>
        <name evidence="4" type="ordered locus">STH606</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH606"/>
    <dbReference type="EnsemblBacteria" id="BAD39591">
        <property type="protein sequence ID" value="BAD39591"/>
        <property type="gene ID" value="STH606"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH606"/>
    <dbReference type="eggNOG" id="COG0363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049611_1_1_9"/>
    <dbReference type="OMA" id="FNEPCSS"/>
    <dbReference type="UniPathway" id="UPA00629">
        <property type="reaction ID" value="UER00684"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01241">
        <property type="entry name" value="GlcN6P_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="PTHR11280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine_iso" evidence="3">
        <location>
            <begin position="9"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for enolization step" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="For ring-opening step" evidence="2">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for ring-opening step" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="For ring-opening step" evidence="2">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="26111" checksum="F2A3EF3FEA747320" modified="2004-10-11" version="1">MEVFPDYAALSQRAAEIVIGLLKEKPDAVLGLPTGSTPVGFYDALVASGVSLARARTFNLDEYLGLPRTHPESYYSFMKRMLYDRTDLRPENCHIPDGTAADPEAECRRYEEAIRAAGGLDILILGVGHNGHIGFNEPGAPWDGRTRIVELAERTRQANARFFNNNIDEVPRRAITMGIGTILEARRLLLLANGAGKAPIVQRLVEGEPTPEVPASVLHYHPDATVLLDTEAASLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX7</accession>
    <name>Q67RX7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH581"/>
    <dbReference type="EnsemblBacteria" id="BAD39566">
        <property type="protein sequence ID" value="BAD39566"/>
        <property type="gene ID" value="STH581"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH581"/>
    <dbReference type="eggNOG" id="COG1878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_0_9"/>
    <dbReference type="OMA" id="HIMEHEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="PTHR31118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="27042" checksum="BFDA174371DDF8F8" modified="2004-10-11" version="1">MASGHTGRPGQQPNAKEVGPLRIIDLTLGYSHGMPAYGTAWYSQVEIRPLMTPETDPTGHGRRFSQFVFNPHNATHVDAPSHFVPGGKNVSDLEPDRFIGPALVLDLTHRGLYEPVTADDLEAAAGGRMRPGLRLLLRTDYVDRHWGDPDFWQKPPYLAPSAADWCVEQGAALVGLDFLTEEPGDRDFPVHRRLLEADIPILEYLRNLKALRGPIVWLMAAPMLVEGAEAAPVRALAVEGGVPYGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY1</accession>
    <name>Q67RY1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 3-oxoacyl-(Acyl-carrier protein) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194711.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH577"/>
    <dbReference type="EnsemblBacteria" id="BAD39562">
        <property type="protein sequence ID" value="BAD39562"/>
        <property type="gene ID" value="STH577"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH577"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="25439" checksum="850A057A3F25D129" modified="2004-10-11" version="1">MDLSGKRALVTGASKGLGAAVARRLAQAGADVAVHYHRDKAGAEETAKAVNASGRRALVLRADLAVPGQAESVVELVRDAWGGLDLLVNNAGVAPVLPWDQVTPEQWAAVLAADLSGPFHVMRAALPLLREGREPAVVNVGSVVSFNGGAFGPAYAAAKAGLVGLTRSAAREWGPLGIRVNCVAPGPIDSPLARALPTPALEAMKAQTPLRRLGTFGEVAEVVAWLLSPAAAFVTGQTVVVDGGRVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY9</accession>
    <name>Q67RY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH569</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH569"/>
    <dbReference type="EnsemblBacteria" id="BAD39554">
        <property type="protein sequence ID" value="BAD39554"/>
        <property type="gene ID" value="STH569"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH569"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_894080_0_0_9"/>
    <dbReference type="OMA" id="GCSERRI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="98"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="33789" checksum="B71D7DD379D1EC20" modified="2004-10-11" version="1">MIGLTWLPAGVFWVEEGDLPVGVVWGVRTESMQPFILEPEPVARKPRAHWSVSQYGQAVVTYVRELVTGQVTDYSHRWTESGPVQIPEPVLPQVRERMVVSLRLLSRALVLGMAAGFALGVLSLRRGAARGFSLGISVAGMALPEFVVVLLLQMLTIFTYREFGIRLYSVLGPSGGERGWLVPLLALSLGPMAYTARLVAGGLDEVMREDFIRTARAKGLSEARVVMKHGLRHVLGRVLGGFPTIMGNALSSMVIIEVLANVNGLAGGLMANWSSRMVATTGLVFCLWFGLLDALANTLRILANPRLREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S60</accession>
    <name>Q67S60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH498"/>
    <dbReference type="EnsemblBacteria" id="BAD39483">
        <property type="protein sequence ID" value="BAD39483"/>
        <property type="gene ID" value="STH498"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH498"/>
    <dbReference type="eggNOG" id="COG4129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_911926_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010343">
        <property type="entry name" value="ArAE_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06081">
        <property type="entry name" value="ArAE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32760" checksum="8232330C61C2798B" modified="2004-10-11" version="1">MLAVQPSITQAKNAFLQQLVGNAVAGAVATLLGLWLPVNPLTMALGAVLALGILVRMRLTEAAGLAVVVVLFVLDRPQHDFLLYTLARMATIVVGMTVGFLVNRLIHPPDALGRARAELAEGNRELDASIDRLLLSLSHPQDYPAAQLSQDADEARRRLSAARTLLDFAEAEGRAEDAETLREAHAVLEGFVAAVAVIHRIAADMGGVGYGPEREALAAAMRALQAYKGAVLDGVLRGAAVDREAARKCEAALAAFDGLVSALIDRRDRREYGLQLHLILSEMRHMGWRLHPLGRRAETGGRQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S63</accession>
    <name>Q67S63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194629.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH495"/>
    <dbReference type="EnsemblBacteria" id="BAD39480">
        <property type="protein sequence ID" value="BAD39480"/>
        <property type="gene ID" value="STH495"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH495"/>
    <dbReference type="HOGENOM" id="CLU_943107_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269519" evidence="1">
        <location>
            <begin position="28"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="31964" checksum="26A35AE662305D60" modified="2004-10-11" version="1">MRRWLVVLGMVLALLAGCAREEPTAEAAAGAALRFMEALAGGDAGQVWGALTAEARRRMDSGAVASYLADHTVRFEAVGAARELEPGVMRVAVRGVTVRDPGRAVEWSEWSLTLRWEEDRWAVAWAGPLFEPALTAYHNTRYHEQLNLARDIVAIDPYHYRGHLELHYAFRGLGRIRQAEYALNTAWERASAAEKADVMAARARFKLALGAPADALDLAREALDLARPYAPGTYSPSWQAETLVLAAQAALALGDVDAAQALAEEAAAVDADNAAVAVFRYQLAAGGRPQQESTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S80</accession>
    <name>Q67S80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore peptidoglycan hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194612.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH478"/>
    <dbReference type="EnsemblBacteria" id="BAD39463">
        <property type="protein sequence ID" value="BAD39463"/>
        <property type="gene ID" value="STH478"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH478"/>
    <dbReference type="eggNOG" id="COG3858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037415_1_0_9"/>
    <dbReference type="OMA" id="IKTWAVI"/>
    <dbReference type="OrthoDB" id="1081028at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02874">
        <property type="entry name" value="GH18_CFLE_spore_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041704">
        <property type="entry name" value="CFLE_GH18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="2">
        <location>
            <begin position="74"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="390" mass="43539" checksum="87255E3DE3218DC2" modified="2004-10-11" version="1">MSFPDPRQPIPAHPHRPRRRTGWAVLAALLVAAAAGAGGFLLGQYTAPHPPFDPLWLRAYLDRPLDTAPPGEGFWIAGYYVDYDSDSLESVRLNAHHLDQVIVFGYGFDAEGNAVGKDQTLIRGVTSHQKRVLLFGNFSDAGFDPDLAHRILTDPVVQERAMSSMLDEAGRLGVSGIQIDFENIPPEDREAYTQFLRRLKERLEPLGLTLSVAAAAKTSDTTSGWGGATDYAAIGKIVDHFYIMAYDQHWLGGEPGPVASLDWTERVIRYAIGVMPSQKILLGVPLYGYEWALEPELADTNAAYGPGRMQRRAAQFGAEVVWDPVHAENRAVFLTDEGQRVAWFPDERSLEAKLRLAYQYNLKGIAVWRVGLEPDDWWHRMGEFRLNPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S89</accession>
    <name>Q67S89_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spermidine/putrescine ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH469</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194603.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH469"/>
    <dbReference type="EnsemblBacteria" id="BAD39454">
        <property type="protein sequence ID" value="BAD39454"/>
        <property type="gene ID" value="STH469"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH469"/>
    <dbReference type="eggNOG" id="COG1177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_3_0_9"/>
    <dbReference type="OMA" id="NKFGMKW"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="61"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27932" checksum="B5AB14191C272737" modified="2004-10-11" version="1">MKRGSGLLRLHAALVYGFLYLPIFLLVILSFNSSRTSAVWKGFTLDWYVRLFQNRLLWEAVKNSLIIAAATAVIATALGTAAALALHRRHIPGRVWIEGLLYVPLVLPDIVLGVGALVLFTALGISLGLGTILAAHVGTSISYVVLVLRARLSDMDERLEEAARDLGATPWQTLRYVTLPLLMPAIVAAILLSFTLSLDDFILSFFTAGPGATTLPLRVYGMMKTGLTPEVNALSTIMVLVTGIATTLYLRLTENRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC4</accession>
    <name>NADD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="1">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadD</name>
        <name type="ordered locus">STH434</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NadD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194568.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SC4"/>
    <dbReference type="SMR" id="Q67SC4"/>
    <dbReference type="STRING" id="292459.STH434"/>
    <dbReference type="PRIDE" id="Q67SC4"/>
    <dbReference type="EnsemblBacteria" id="BAD39419">
        <property type="protein sequence ID" value="BAD39419"/>
        <property type="gene ID" value="STH434"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH434"/>
    <dbReference type="eggNOG" id="COG1057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069765_1_0_9"/>
    <dbReference type="OMA" id="IHIGHLI"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000310154" description="Probable nicotinate-nucleotide adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <sequence length="208" mass="22845" checksum="38BE92CA06953BEC" modified="2004-10-11" version="1">MARVAVLGGTFDPIHLGHLAAAQGVLHLTGVERVIFLPNRQPPHKQGQPVTPAEHRAAMVRLAIADNPAFGFSDLELRRPGPSYTIETVRALAAEHPDWEPAFIIGLDSLLAIRTWREWETLMQSVDFFAVTRPGHDLAAARRLLAELGPRLSGRVRLLEIPGVAVASADLRRLAAAGYPLRYLVPDPVARYIAEHRLYLRGESHGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM3</accession>
    <name>Q67SM3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH335"/>
    <dbReference type="EnsemblBacteria" id="BAD39320">
        <property type="protein sequence ID" value="BAD39320"/>
        <property type="gene ID" value="STH335"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH335"/>
    <dbReference type="HOGENOM" id="CLU_2262413_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="103" mass="11949" checksum="8ED521A17D76D9A4" modified="2004-10-11" version="1">MIAHNPAYSCLHYSSPPWPAHQTRPDKCLCTTSSCRSKPAKNTKVAYPPERILRPWIWEIGDRYLLLTNDFLFGERLPFFRRRPAIKLLPSLNGNFIIPLVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN1</accession>
    <name>Q67SN1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH327"/>
    <dbReference type="EnsemblBacteria" id="BAD39312">
        <property type="protein sequence ID" value="BAD39312"/>
        <property type="gene ID" value="STH327"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH327"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="285"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="541" mass="60901" checksum="E3749F597201FA51" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV7</accession>
    <name>Q67SV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194386.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH251"/>
    <dbReference type="ESTHER" id="symth-q67sv7">
        <property type="family name" value="CarbLipBact_2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39236">
        <property type="protein sequence ID" value="BAD39236"/>
        <property type="gene ID" value="STH251"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH251"/>
    <dbReference type="eggNOG" id="COG1647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076594_0_2_9"/>
    <dbReference type="OMA" id="PEIDIMT"/>
    <dbReference type="OrthoDB" id="1694750at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012354">
        <property type="entry name" value="Esterase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013595">
        <property type="entry name" value="Pept_S33_TAP-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08386">
        <property type="entry name" value="Abhydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017388">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="21"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Abhydrolase_4" evidence="3">
        <location>
            <begin position="161"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR017388-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="228" mass="24151" checksum="033FB3FFC993D402" modified="2004-10-11" version="1">MPIARRSREAVYLRAGPVGCLVLHGFGGSPAEVRPLVEGLAARGITVCAPVLPGHGPDAANLGRTRLRHWIRGAEGALRELQAACAEVHVAGFSLGGLIALHLAARHPVSSVCTLAAPILAEDPGAIGARLSAYLGDPAAAMPALRSLVRLARLARRDLGRVRAPVLVLQGDRDDWIAPESGQYLAERLSSARLVVLPGRGHFLALERGREEVAVRIAQWITARSEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY9</accession>
    <name>Q67SY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HtrA family serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH219"/>
    <dbReference type="EnsemblBacteria" id="BAD39204">
        <property type="protein sequence ID" value="BAD39204"/>
        <property type="gene ID" value="STH219"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH219"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_2_2_9"/>
    <dbReference type="OMA" id="AQHERPL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="212"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="35454" checksum="1E214996315E791B" modified="2004-10-11" version="1">MGVDPFSSALIAALDRVRPAVVHIYAATPKTGQVALGTGVILDHYHIISTAQIVAPEDEITIKTADGKRRRAECIGVDPLYFLTVLQVEERLPHDPPTFAPDGTTPVGLFVAAVGYALGQEHTAASGIISSSDRTVYRPKARGRGNLPVDGLLLTSAAIHPGNAGGPLIDLEGRVVGINGIPWQGGLSLAIQASVAARVASQIIDYGYAVHPWLGFSGEVDVIDDIWVEMLNLPTDRGLVVQHVASDGPGRRAGVQEMDMIMAVGDRQPVLSTGMIRKLLSARRYGDQVPMTVLRQGELIELRLPVEEIPGLDRFDPVSDDDESAAPDDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T80</accession>
    <name>Q67T80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH128</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH128"/>
    <dbReference type="EnsemblBacteria" id="BAD39113">
        <property type="protein sequence ID" value="BAD39113"/>
        <property type="gene ID" value="STH128"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH128"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_3_9"/>
    <dbReference type="OMA" id="VIEYMAR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="4">
        <location>
            <begin position="95"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="543" mass="60826" checksum="8E9FF749BF1E0CB7" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA0</accession>
    <name>Q67TA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH108"/>
    <dbReference type="EnsemblBacteria" id="BAD39093">
        <property type="protein sequence ID" value="BAD39093"/>
        <property type="gene ID" value="STH108"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH108"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1467498_0_0_9"/>
    <dbReference type="OMA" id="MASHRNM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269369" evidence="1">
        <location>
            <begin position="47"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="97"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="19456" checksum="C72A540742AA86B4" modified="2004-10-11" version="1">MSEGGHRMANLKRRTALSLALVFALAALAACGGSAQQPVATNQAAAAPTQECPPCPEPEPCDEEAILLEAAQDYFNNMASHRNMIDAAEVKEKLEAGDDSIFLLDIRADADFAAGHVEGSVNIPFAALGMNFDKLPTDKLIVVNCYSGQQSGQAAAVLRMAGYNAMSLKGGFPNYEKAELPIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF4</accession>
    <name>Q67TF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH54</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH54"/>
    <dbReference type="EnsemblBacteria" id="BAD39039">
        <property type="protein sequence ID" value="BAD39039"/>
        <property type="gene ID" value="STH54"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH54"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038364_2_0_9"/>
    <dbReference type="OMA" id="LETDYHR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015378">
        <property type="entry name" value="Transposase-like_Mu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09299">
        <property type="entry name" value="Mu-transpos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="16"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="37943" checksum="8ED95ED2C5386228" modified="2004-10-11" version="1">MTGIRSRKRRATFRRFEAAHRNDCWQGDVCHLLNLPHPDRPGKTRKVYLIAFIDDYSRIVYGQMYWEEKLPRLEDCLKRTILRYGLPRRIYVDNGAIYSARHLARICGRLGIRLTHSRPYRPQGRGKIERFFLTVQKSFLPEAQQLIAAGQLATLEQLNELFWAWLEVAYHNRVHGVTKQTPRQRFEGDTEPLRRIDPSALREVFLWEEERVVDQTGCFSLYRNQYEVDPALARRKVTLRYDPYDLSQIQVWHEGKRFADAVPLKLHRTRHRSVSPAPEPATAAPKTGLNFLTLAKKQHDTERRRRLGETAYTRLTQPKGGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH0</accession>
    <name>Q67TH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysine 5,6-aminomutase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH38</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194173.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH38"/>
    <dbReference type="EnsemblBacteria" id="BAD39023">
        <property type="protein sequence ID" value="BAD39023"/>
        <property type="gene ID" value="STH38"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH38"/>
    <dbReference type="eggNOG" id="COG5012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065773_0_0_9"/>
    <dbReference type="OMA" id="AIMNMKG"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028991">
        <property type="entry name" value="KamE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036843">
        <property type="entry name" value="KamE_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16554">
        <property type="entry name" value="OAM_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117778">
        <property type="entry name" value="SSF117778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="1">
        <location>
            <begin position="103"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="26577" checksum="DBBBFE5E1FF51259" modified="2004-10-11" version="1">MSRVVLPYGDTTGDGAVQLSFTLPVPAGSLAREAARQLCLKMNLDNPQVVAMRDLGGGYTFFVVYAKATQGVDLEAIRVPEVEAPKWSMEEIDRMIADRLGRPIRVVGACTGTDAHTVGLDAILNMKGYRGDYGLERYQGFRVWNLGSQVRNEVLVAKAREVEADAVLVSQVVTQKNVHLSNLTGLVDLLEAEGMRDRVILIAGGPRITHELAVELGYDAGFGPGTLPSQVAAFIVKTLLERQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ0</accession>
    <name>Q67TJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arginase-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH18</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH18"/>
    <dbReference type="EnsemblBacteria" id="BAD39003">
        <property type="protein sequence ID" value="BAD39003"/>
        <property type="gene ID" value="STH18"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH18"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_9"/>
    <dbReference type="OMA" id="YELTTIM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="32456" checksum="18000BB727CA6200" modified="2004-10-11" version="1">MSPRSLPGFPHIERLADFMAASDDYATARAVLWGIGQDFTTSYRPGTRFGPGRIREASYGIEEFSYHSRMSLTDKNFFDLGDVAVVFGDVQESLRRAEEVARRLFADGKLSLMMGGEHLVTLPVVKAAYEKYGDDLVLLQFDAHADLREDYLGNPLSHATVMRRCLDFLPAQNLYQFGIRSGTREEYEFGTSRCHLFPHQVLEPLRQVIPTLGDRPVYVTIDIDVMDPAFAPGTGTPEPGGITSREMIDAVLAMRGLNVVGLDVVEVAPGLDQTDRTPVLAAKLIREAILAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA4</accession>
    <name>Q67JA4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3265"/>
    <dbReference type="EnsemblBacteria" id="BAD42246">
        <property type="protein sequence ID" value="BAD42246"/>
        <property type="gene ID" value="STH3265"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3265"/>
    <dbReference type="eggNOG" id="COG2959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045803_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014239">
        <property type="entry name" value="Spore_YpeB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14620">
        <property type="entry name" value="YPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="435" mass="48244" checksum="2469EB13F880EB95" modified="2004-10-11" version="1">MGFGAYQTRLKNQYLREAENKYMAAFHKLKWTSENIEERMAKLMATNDPRLQESLLADLRVFSAQAVEHMATLPFTTMNTPRITNFLNTLRAQSDEKHHKLNTGQGLTDEDWNQLMELRHQAVYFEQELSNLLGLVGNNLIRWQPTVQATSPAQSGQASTPITKSVMLLEENLPVPPGEENALAPEKAQLPRPQTDPGPRVDAAAAAEAIKRFVDMPLAGEPVQTGVYDPEDTEKGLSLYYFDARKQNGLPMNFGVSVHGGHVVYMIDGRAVTEKKLSHEELIAKAKELLAKWGFDQVALVSSAENDGTLVMDFAPVAGGVAVHTEMIRVMLAMDNGELVGYDARAYWVNHVERDLPAPQIAAAEAAARTSPRLTVTGEPRLALIADRRGQERLVWEVPGRVEDQYFRVFVDAMDGTEVDLIRSVGDPAPPMQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF9</accession>
    <name>Q67JF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3209</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3209"/>
    <dbReference type="EnsemblBacteria" id="BAD42191">
        <property type="protein sequence ID" value="BAD42191"/>
        <property type="gene ID" value="STH3209"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3209"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018240_2_1_9"/>
    <dbReference type="OMA" id="EIGACRM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036991">
        <property type="entry name" value="Fe_hydrogenase_ssu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013352">
        <property type="entry name" value="Fe_hydrogenase_subset"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="SSF53920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02512">
        <property type="entry name" value="FeFe_hydrog_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="8">
        <location>
            <begin position="86"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="188"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="596" mass="64393" checksum="BAA24CCE94939839" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN4</accession>
    <name>MCSB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-arginine kinase</fullName>
            <ecNumber evidence="1">2.7.14.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mcsB</name>
        <name type="ordered locus">STH3134</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific phosphorylation of arginine residues in proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-arginyl-[protein] = ADP + H(+) + N(omega)-phospho-L-arginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:43384"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10533"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83226"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.14.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Appears to be allosterically activated by the binding of pArg-containing polypeptides to the pArg-binding pocket localized in the C-terminal domain of McsB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.14.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JN4"/>
    <dbReference type="SMR" id="Q67JN4"/>
    <dbReference type="STRING" id="292459.STH3134"/>
    <dbReference type="EnsemblBacteria" id="BAD42116">
        <property type="protein sequence ID" value="BAD42116"/>
        <property type="gene ID" value="STH3134"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3134"/>
    <dbReference type="eggNOG" id="COG3869">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066591_1_0_9"/>
    <dbReference type="OMA" id="IIMQERV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07930">
        <property type="entry name" value="bacterial_phosphagen_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00602">
        <property type="entry name" value="Prot_Arg_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023660">
        <property type="entry name" value="Arg_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="PTHR11547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000212038" description="Protein-arginine kinase">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="1">
        <location>
            <begin position="24"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RDXXRA motif of the pArg binding pocket involved in allosteric regulation" evidence="1">
        <location>
            <begin position="339"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="27"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="178"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="209"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00602"/>
        </source>
    </evidence>
    <sequence length="353" mass="39405" checksum="33E19F14BC005834" modified="2004-10-11" version="1">MGWNELVSRAESRWMEGTGPHADIVLSSRIRLARNLDDLPFPQRMSEPDTERLLQAAEAGVREINLVGFPSRVELYRLADTTPLDRQILVEKHLISPQQSKEVMAKAVAISEDEAISIMVNEEDHLRIQVLASGLQLQEAWRVASQVDDALEQRLQFAFDEQLGYLTACPTNVGTGLRASVMMHLPALVLTQQAGRLFHNLSQLGLVVRGLYGEGTEAAGQIFQISNQTSLGKAEEEIIANLEAIARTVIDTEEQARRHLYGEMRLQIEDRVSRAYGILTTARMISSEEAMRLLSDVRLGVALGVVPKIDYRILNELLVAMQPACLQRQAGRELNPLERDVRRAALIRARLSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP9</accession>
    <name>SYC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="1">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cysS</name>
        <name type="ordered locus">STH3119</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JP9"/>
    <dbReference type="SMR" id="Q67JP9"/>
    <dbReference type="STRING" id="292459.STH3119"/>
    <dbReference type="EnsemblBacteria" id="BAD42101">
        <property type="protein sequence ID" value="BAD42101"/>
        <property type="gene ID" value="STH3119"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3119"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_9"/>
    <dbReference type="OMA" id="AKYWMHN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159501" description="Cysteine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="269"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <sequence length="471" mass="53307" checksum="1E85CC1C04622D51" modified="2004-10-11" version="1">MGIRIYNDLTRKKEDFVPLEPGKVRFYNCGPTVYDYFHIGNARNFVVFDTVRRYLEYRGYQVTFVQNFTDIDDRMIKRARERGITVSELAEEMIQAYFADAGALGVRPADVHPRATALIDEQIAMIQQLIDKGHAYVVEGGDVYFRVTTSPDYGKLSHKNLEELVAGASERVDPDDRKEHPFDFALWKGQKPGEPAWPAPWGPGRPGWHIECSAMARKYLGDTIDIHAGGEDLTFPHHENEIAQSEAVTGKPFARYWMHNAHLMIDGAKMSKSVGNFFTVRDILKRYDGEVVRMFLLSAHYRTQLSFSDQLMEDTRRALERLYNTVANLEHLAKTAPRAEMTAEEQAVLAELSQARERFVAAMDDDFNTAEGLAVIFDLSRELNSRVKPGASAAFAEGGLALLRELAGVLGLLERKAQPQELDAEIEALIAARQEARKARNFAEADRIRDQLRAMGIILEDTPQGVRWRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ7</accession>
    <name>Q67JQ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3111"/>
    <dbReference type="EnsemblBacteria" id="BAD42093">
        <property type="protein sequence ID" value="BAD42093"/>
        <property type="gene ID" value="STH3111"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3111"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108859_2_1_9"/>
    <dbReference type="OMA" id="KCGFRIQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="17289" checksum="71801D836F3BA29A" modified="2004-10-11" version="1">MDDLMIRQMRHDEEPPMDLLLMADPSREMVAGYLGRGRCYVGERAGRVVAVCVLIDTRPYTAELVNVAVAEEERGRGIGKRMVLHAIAVARSLGYRCIELGTGNSSVDQLALYQKCGFRIVGVDPDYFTRHYPEPIYENGIPCRDMLRLRLDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV0</accession>
    <name>RL16_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplP</name>
        <name type="ordered locus">STH3068</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197183.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV0"/>
    <dbReference type="SMR" id="Q67JV0"/>
    <dbReference type="STRING" id="292459.STH3068"/>
    <dbReference type="EnsemblBacteria" id="BAD42050">
        <property type="protein sequence ID" value="BAD42050"/>
        <property type="gene ID" value="STH3068"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3068"/>
    <dbReference type="eggNOG" id="COG0197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_2_1_9"/>
    <dbReference type="OMA" id="KGAVEYW"/>
    <dbReference type="OrthoDB" id="1786618at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000062228" description="50S ribosomal protein L16">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <sequence length="155" mass="17039" checksum="159F3BAD977A2CA9" modified="2004-10-11" version="1">MLAPKRVKWRRVHKAVAKLNKGRSKAGNEVVFGDYGLQALEPAFITDRQIEAARVAMTRFIKRGGKVWIKIFPHKALTRKPAEVRMGSGKGAPEVWVAVVKPGRILFELAGVDEETAQEAMRLAAHKLPIKCKFVKREKAVTPAAAETGGEANEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ7</accession>
    <name>Q67JZ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197135.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3020"/>
    <dbReference type="EnsemblBacteria" id="BAD42003">
        <property type="protein sequence ID" value="BAD42003"/>
        <property type="gene ID" value="STH3020"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3020"/>
    <dbReference type="HOGENOM" id="CLU_990197_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="30232" checksum="729E696F8BA409B6" modified="2004-10-11" version="1">MHLNRFWSSVWTVAGFLLLITLLQMIADQVVTLFGLLGSVAPSAARWAVAASHAVVPIAASLTYIDWRWGWKADNAGLRPTFAARFWFLPGLAGGLALAGLAFLLGGWGRMPQLNLGVESLVQAALTSAALFGVELILRGIAISRFEQDLSGRDVLLLALVTPVAWVVLTALFGLGWPAPQIRAQFGGLAGLTFSALQSLLFLQTQSVWLVAGVHIGLYLGRNVLGLDYGQAGLLLVTAVPAALLLWRELNRMRTIRPAGPRPGKRRVAYGKTVRGPWGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K34</accession>
    <name>Q67K34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197096.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2981"/>
    <dbReference type="EnsemblBacteria" id="BAD41964">
        <property type="protein sequence ID" value="BAD41964"/>
        <property type="gene ID" value="STH2981"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2981"/>
    <dbReference type="HOGENOM" id="CLU_2107755_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="13049" checksum="8FEBE7A9ADE9D8DD" modified="2004-10-11" version="1">MSASALLREMIELTRLQVQRVIQRDHQGLLEGSRQQEALLKALQEAEIDGSPEKMRAIYEELEREKVKLQSLLEAESQRVDFLMRLLLGGPELNSVGYPSTVTKRAGGRMLNKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K35</accession>
    <name>Q67K35_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar hook-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flgK</name>
        <name evidence="8" type="ordered locus">STH2980</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197095.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2980"/>
    <dbReference type="EnsemblBacteria" id="BAD41963">
        <property type="protein sequence ID" value="BAD41963"/>
        <property type="gene ID" value="STH2980"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2980"/>
    <dbReference type="eggNOG" id="COG1256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012762_1_1_9"/>
    <dbReference type="OrthoDB" id="1815809at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002371">
        <property type="entry name" value="FlgK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30033">
        <property type="entry name" value="PTHR30033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02492">
        <property type="entry name" value="flgK_ends"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flg_bbr_C" evidence="7">
        <location>
            <begin position="508"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="558" mass="62120" checksum="E1AE337799049EF8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG0</accession>
    <name>PUR5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="1">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purM</name>
        <name type="ordered locus">STH2853</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196972.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KG0"/>
    <dbReference type="SMR" id="Q67KG0"/>
    <dbReference type="STRING" id="292459.STH2853"/>
    <dbReference type="EnsemblBacteria" id="BAD41838">
        <property type="protein sequence ID" value="BAD41838"/>
        <property type="gene ID" value="STH2853"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2853"/>
    <dbReference type="eggNOG" id="COG0150">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_9"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="OrthoDB" id="923547at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000258420" description="Phosphoribosylformylglycinamidine cyclo-ligase">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <sequence length="353" mass="37409" checksum="F54688ECDB4AEF3F" modified="2004-10-11" version="1">MTEKGLTYADAGVNRERHYELVRRIAAHTARTLRRPGTLGNIGAFGGLFQLDPARYPEPVLVSGTDGVGTKLRLAFLSGRHDTVGIDLVAMSVNDILCQGAEPLFFLDYIGIGQKDLAVLEQVVKGIADGCLQAGCALIGGETAELPGMYPPGEYDLAGFAVGIVNRDRLLTGEKVAPGDALVGLASSGLHANGYSLARRVLLKVDGGAFDLDDRPPELGGRTVLEVMLTPTRIYVRTVLRLLARFDVHGIANITGGGLHENIPRMLPEGTAAVLRRGAWKEPPVFDLIRRLGPVAQAEMEATFNLGLGMVLAVPADQAEAVAAAARELGEEAWVVGEVAAAEPGGPRVVVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG1</accession>
    <name>Q67KG1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="3">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">GAR transformylase</fullName>
            <shortName evidence="3">GART</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purN</name>
        <name evidence="5" type="ordered locus">STH2852</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196971.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q67KG1"/>
    <dbReference type="STRING" id="292459.STH2852"/>
    <dbReference type="EnsemblBacteria" id="BAD41837">
        <property type="protein sequence ID" value="BAD41837"/>
        <property type="gene ID" value="STH2852"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2852"/>
    <dbReference type="eggNOG" id="COG0299">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_1_0_9"/>
    <dbReference type="OMA" id="TGITIHY"/>
    <dbReference type="OrthoDB" id="1815747at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="3">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="10-formyltetrahydrofolate binding" evidence="3">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="site" description="Raises pKa of active site His" evidence="3">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="22602" checksum="AA7E0B6353B78798" modified="2004-10-11" version="1">MIRIGVLISGSGTNLQAILDGCREGRIPGRVAVVISDRADAYGLERARRAGVDALHMDPAAYPSRTAFDAALAERLQAYGVDLVCLAGYMRLVRGPMLTAFPNRILNIHPSLLPAFPGLEAQRQALEHGVKVAGCTVHFVTAGVDEGPIILQAAVPVLEGDTVEDLRRRILAEEHRIYPEAIRLFAEGRLVIEGRRVRILDRAEAPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL1</accession>
    <name>Q67KL1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196921.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2802"/>
    <dbReference type="EnsemblBacteria" id="BAD41787">
        <property type="protein sequence ID" value="BAD41787"/>
        <property type="gene ID" value="STH2802"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2802"/>
    <dbReference type="HOGENOM" id="CLU_2425880_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="9801" checksum="4081CF0D86BBD4C6" modified="2004-10-11" version="1">MNRMLHETLAIVGWSFLGATAIGVVGALLFRWPITVTYMLSVLTIGPALAVLRGRDRSRDPLELGERAGVELVAILILLGIGWLAGQYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM2</accession>
    <name>Q67KM2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Yip1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2791</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196910.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2791"/>
    <dbReference type="EnsemblBacteria" id="BAD41776">
        <property type="protein sequence ID" value="BAD41776"/>
        <property type="gene ID" value="STH2791"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2791"/>
    <dbReference type="eggNOG" id="ENOG5033IVY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1234489_0_0_9"/>
    <dbReference type="OMA" id="IGLREAH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="6">
        <location>
            <begin position="19"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="23034" checksum="40B58D0D80548AFF" modified="2004-10-11" version="1">MTEPSAQNGATSVWDLMLDVFIAPGRAMAKIADERPVGAAAWVVTGLAVFTGLLGMASARDAMALDPGLLPSDVPPEVVEVIQALTGSASVFGAVVGILASVLWWFGRAAIYGLIGELMGAARDGRAMLATLGFAGLPALLQAPLNLMLSRLGLTWLSALVGIVFWVWLLVLTVLALRAALRVDTGQAVVIFLIPVGVAVALAVLVGVGLAVALVSMVGRIPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ6</accession>
    <name>Q67KQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2756"/>
    <dbReference type="EnsemblBacteria" id="BAD41741">
        <property type="protein sequence ID" value="BAD41741"/>
        <property type="gene ID" value="STH2756"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2756"/>
    <dbReference type="eggNOG" id="ENOG502Z7J9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1019163_0_0_9"/>
    <dbReference type="OMA" id="GQYLIFE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021246">
        <property type="entry name" value="DUF2797"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10977">
        <property type="entry name" value="DUF2797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28622" checksum="157ACB381008DD26" modified="2004-10-11" version="1">MIRDLLLRIGDGVVDYHLLLDEQEIALNPLLGGRVAIRFTGERRCVYCGRRASKLFNNGSCWPCFRRLAINDLCQVKPTLCHYETCREPEWGDAHCMIPTYVYLARSSDVKVGITRSLPGRWLDQGAVEAVPIARVPNRKMAGELEAFLTQYVADKTNWRRMLKGEVADADLLAERSRLLELIPAEFRPYVLPDEEVRSFTYPLKAPPPKLASCDLEKGDVEGTLLGMKGSYLVLDTGVLNVPKFAGYVVTFEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS1</accession>
    <name>Q67KS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">1-pyrroline-5 carboxylate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2741</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196860.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2741"/>
    <dbReference type="EnsemblBacteria" id="BAD41726">
        <property type="protein sequence ID" value="BAD41726"/>
        <property type="gene ID" value="STH2741"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2741"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_9"/>
    <dbReference type="OMA" id="TAGICTD"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07124">
        <property type="entry name" value="ALDH_PutA-P5CDH-RocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005932">
        <property type="entry name" value="RocA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01237">
        <property type="entry name" value="D1pyr5carbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="52"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="520" mass="56819" checksum="3E6F6A69BE4B9CE3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV3</accession>
    <name>Q67KV3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative polysaccharide polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41693">
        <property type="protein sequence ID" value="BAD41693"/>
        <property type="gene ID" value="STH2708"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2708"/>
    <dbReference type="eggNOG" id="ENOG502ZV6S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648790_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="423" mass="46043" checksum="AEA7B06F98A0E542" modified="2004-10-11" version="1">MEHSRSGLSHALLYMSTSKLVHQVLCLLVAVLPFLPIWKRMTSRGGISWSGALVEVALFGLLGVASILSLTRTKFWLARRVLPFLAVTFIIGLIQTCRSSVGLIGAADVFRLYLEPLVVFLAVHSLPTDHYRIQVAKVIARVGLFLAVIGMIQLVAPEPIRQFHIVTGADPLVLSTKSNFMAGSQYNRVISFLDDPNIASQVFIAGLTAQLYLFLRRFETKWLVSVVISSSAVIATGSRSGLLSFAAALGCLLLYVWLSSNKRLKARALWAGVSGLLLLTLPLSVGNIRAIALDRFGRFGVSALAADGRLENWQQVMREVSGSIWTVLFGTGLTPGDLVTSTIVENAYLSALRDFGVIWLIVLCAAFLIAFLPIIRKSSFFGIVVLAPFLVANIFGDYTKIPLVSCWYAVMLGLSHKSGVSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L06</accession>
    <name>Q67L06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196777.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2655"/>
    <dbReference type="EnsemblBacteria" id="BAD41640">
        <property type="protein sequence ID" value="BAD41640"/>
        <property type="gene ID" value="STH2655"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2655"/>
    <dbReference type="eggNOG" id="COG4447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1133137_0_0_9"/>
    <dbReference type="OrthoDB" id="1293876at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="SSF50939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="26172" checksum="83BB78FEE98A189A" modified="2004-10-11" version="1">MSLAYGAGRFVACADGGLSASTDGLTWTPVPLDARYWHIDLSCTGKEFVATGCITSRTCEVILTSADGVSRYQRVGLAAFDQVAYGNGRYVLSFGNAAWTWSSADLTNWTTVNTLLFLALTFDQGMFVAADADEGTIRTSADGVEWTEVFHPDQPVYISDLAYGGGRFVAVGGTNSGPRRAVVLTSGDGRQWSLQVQEEAEGLVRVVYGAGRFVANSEAALAVSEDGTGCRVQVLRGDEVRARRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L11</accession>
    <name>Q67L11_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2650"/>
    <dbReference type="EnsemblBacteria" id="BAD41635">
        <property type="protein sequence ID" value="BAD41635"/>
        <property type="gene ID" value="STH2650"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2650"/>
    <dbReference type="eggNOG" id="COG3595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_768889_0_0_9"/>
    <dbReference type="OMA" id="NSAMEVA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="41570" checksum="78498A84571C2085" modified="2004-10-11" version="1">MKGPGADARKGGFRMDATPRRVGRATVAIGFVATGAALLADNVAGAGPRFTDLLLRLWPVFLIGFGLEFLVSGLLSQAGQPRPVRIEVGGAALLAGALALASVIRATSGFGWHSAPIPEVTSHTVTRSVPAAGAEALLVDVDVGRVSLLTYDEEEVRVEVEYGVLGLVPPNRTAAAAREYRLEVEGGRTIRVTGRGPDENPESGLSLGTLTAHYRIYAPAGLNVRVESGVGAISVEGYNGSLDLRTRAGGITVEGGSGTLYAESGSGRVHVDSFSGPVTASTNAGPVTARAVEGDLTIRTGTGVINVEEHRGPSLTAETRTGSVHARMEHPPAGPVVLRTRAGTISLTVPAESDVTVTATTRSGTITGLGAAGGTGPARATTQILGAGTYPVRLEANAGSIHFDTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L22</accession>
    <name>Q67L22_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Succinate dehydrogenase flavoprotein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196761.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2639"/>
    <dbReference type="EnsemblBacteria" id="BAD41624">
        <property type="protein sequence ID" value="BAD41624"/>
        <property type="gene ID" value="STH2639"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2639"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_2_9"/>
    <dbReference type="OMA" id="HWEWHMF"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="9">
        <location>
            <begin position="7"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="10">
        <location>
            <begin position="449"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="573" mass="62504" checksum="872131AF43FEC2CC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L25</accession>
    <name>Q67L25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">2-oxoglutarate/malate translocator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2636</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196758.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2636"/>
    <dbReference type="EnsemblBacteria" id="BAD41621">
        <property type="protein sequence ID" value="BAD41621"/>
        <property type="gene ID" value="STH2636"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2636"/>
    <dbReference type="eggNOG" id="COG0471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_7_3_9"/>
    <dbReference type="OMA" id="GLNPNAW"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030676">
        <property type="entry name" value="CitT-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
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        <property type="entry name" value="PTHR42826"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="472" mass="50569" checksum="E53C51DFBAA223AE" modified="2004-10-11" version="1">MRNDDRSKLIRGLICVAVGLIIWFLPAPGDLEPVAMHLLGIFVATILGLILQPLPQGAVVITAVAVTAITGTLSIGDALNGFKDSTVWLIVTAFLFARGLIKTGLGRRISFMLVKRFGKSTLGLGYALALSDTVLAPATPSNTARAGGILFPIVRALCSSMGSEPGPTAKRVGQYLMYNSFQICCITSALFFTGVAPNSLIATLAKENFGYEITWLGWLWAALLPGVICIAILPRIYYKIFNPELKYSPEAPALAEKELAKMGPMSTAEKGMLAVFLLTLVLWATSQWTNLNATAIALLGVAILLVIGVLDWQDVLAEKGGWDALIWFGGLVGLATGLSKVGIIGWLASILETSLGGISSWFLAFILVVIAYTYSHYFIASMTAHATAFFVPLALVAISAGAPLEITVLVLGFFNSLNAVMTHYGTGPAPIYFGAGYIDQGTWWKWGFVTSLINLVVWLGAGSLWWKVIGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L42</accession>
    <name>Q67L42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2619"/>
    <dbReference type="EnsemblBacteria" id="BAD41604">
        <property type="protein sequence ID" value="BAD41604"/>
        <property type="gene ID" value="STH2619"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2619"/>
    <dbReference type="HOGENOM" id="CLU_3189889_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="46" mass="5158" checksum="55C6BB6649EAB58C" modified="2004-10-11" version="1">MSMMEILVVLGLLSIVLFAPTSPEIRRRELQYGFGVLTPEAVARPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L60</accession>
    <name>Q67L60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formate dehydrogenase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2601"/>
    <dbReference type="EnsemblBacteria" id="BAD41586">
        <property type="protein sequence ID" value="BAD41586"/>
        <property type="gene ID" value="STH2601"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2601"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_1_2_9"/>
    <dbReference type="OMA" id="KWDGAKW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047111">
        <property type="term" value="F:formate dehydrogenase (cytochrome-c-553) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006443">
        <property type="entry name" value="Formate-DH-alph_fdnG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01553">
        <property type="entry name" value="formate-DH-alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="2">
        <location>
            <begin position="2"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="3">
        <location>
            <begin position="666"/>
            <end position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="799" mass="89407" checksum="0A6C1F9FACB8DDC2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD1</accession>
    <name>Q67LD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2530"/>
    <dbReference type="EnsemblBacteria" id="BAD41515">
        <property type="protein sequence ID" value="BAD41515"/>
        <property type="gene ID" value="STH2530"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2530"/>
    <dbReference type="HOGENOM" id="CLU_1539260_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="19740" checksum="2E03646C3BA5479E" modified="2004-10-11" version="1">MRHSLPRHRAILPVEVRTMPRSYERVLAVLPYIGAIRLPVRPWVIPEWLYTVPAGLVMAGLLWLGARGRSPFVSYHARTGLLWALQANGLLTALSLLAELWYRAWYHTGVPLWNQVWHLNAELFRWAAVLTTLLTGLAMWRAAKGQTGDPLGLPSPSFFRGHDVHLEGSEEGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE4</accession>
    <name>Q67LE4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196640.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2517"/>
    <dbReference type="EnsemblBacteria" id="BAD41502">
        <property type="protein sequence ID" value="BAD41502"/>
        <property type="gene ID" value="STH2517"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2517"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="HPPVYAP"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="241" mass="26132" checksum="3382E5BDF05CBA69" modified="2004-10-11" version="1">MIKARKVSYAYGETVALRDVSFDLPRGQTLAVVGPSGCGKTTLLGLIAGLLPITAGELLVDGAPVQPRRPGTALILQDYGLLPWKTVRENAALGLRIRRLPVDAADQALREVGIADLARRWPHQLSGGQQQRVAIARALALGPDLLLMDEPFSALDALTREEMQDLLLAIWRKRQTSLVLVTHSITEAVYLGQQILVLSRRPGRVVDLFANPAVGDRRADAFHSMVNRVRDALEKGVNGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF7</accession>
    <name>Q67LF7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41489">
        <property type="protein sequence ID" value="BAD41489"/>
        <property type="gene ID" value="STH2504"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2504"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259775_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="22843" checksum="2CBFBD2853073328" modified="2004-10-11" version="1">MNRRYLILSPLVIIAVCHVAARIARQALGPWGWAPAVVLYWGLLGATIAMSGRTGALRRWTAPARSARGWTALALLVGLIPLPILAQNLHLLADPVILAAWLLFALINPWFEEFYWRGLLLDATARWPAWAGTAYSTALFVAFHPAVAGVFSAANRDPVALAALGLMGLAWAAAYRRTGTLRWAVASHVLVDLGNLAVPVFLNLYVPPHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH8</accession>
    <name>Q67LH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenylacetic acid degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">paaD</name>
        <name evidence="2" type="ordered locus">STH2483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196606.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2483"/>
    <dbReference type="EnsemblBacteria" id="BAD41468">
        <property type="protein sequence ID" value="BAD41468"/>
        <property type="gene ID" value="STH2483"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2483"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082133_0_0_9"/>
    <dbReference type="OMA" id="EPFDHFK"/>
    <dbReference type="OrthoDB" id="1668114at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011883">
        <property type="entry name" value="PA_CoA_Oase4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02159">
        <property type="entry name" value="PA_CoA_Oxy4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="17708" checksum="A13014F6378FF77E" modified="2004-10-11" version="1">MVTEGAIWAALADVRDPEIPPVSIVDMGMVHRVTVAGPRVRVEIMPTFVGCPALEIIRRDAEDRLRAVPGVEEVEVAFVLDPPWSSDRITAEGRRRLRSFGIAPPPPGSAGAPLLPAPEEVPACPYCGSTDTHLENLFGPTACRSIYYCDGCRQPFERMKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK5</accession>
    <name>Q67LK5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF2179 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2456</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2456"/>
    <dbReference type="EnsemblBacteria" id="BAD41441">
        <property type="protein sequence ID" value="BAD41441"/>
        <property type="gene ID" value="STH2456"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2456"/>
    <dbReference type="eggNOG" id="COG1284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063199_1_1_9"/>
    <dbReference type="OMA" id="PQRIANE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019264">
        <property type="entry name" value="DUF2179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003740">
        <property type="entry name" value="YitT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10035">
        <property type="entry name" value="DUF2179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02588">
        <property type="entry name" value="YitT_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006483">
        <property type="entry name" value="Membrane_protein_YitT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2179" evidence="8">
        <location>
            <begin position="212"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="29428" checksum="3FBFD9548394298A" modified="2004-10-11" version="1">MLWILQLLAGALLMALSANVFLVPLKLAEGGVTGIGIILYHLLGVPMWVTQVVVNIPILALGLKVKGWRLIRRSLIGVAAYSFFLAVTANVPPITDQVVLAIIYGGLTMGIGLGLVLRSGGSTGGTEVLALVLQQRSGFSVGSMVLAVDTVVLAMAAVAFSPEAAMWAVITLIISSKVVDVVQVGLYSARGVTIITTRPEPIARRIMNEVERGVTIINGTGAFTGEPRTILYTVAQRTELTQVKQIAYAEDPKAFVVVAEVHEVLGEGFRDPSKDMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR0</accession>
    <name>Q67LR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP/GTP-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2401"/>
    <dbReference type="EnsemblBacteria" id="BAD41386">
        <property type="protein sequence ID" value="BAD41386"/>
        <property type="gene ID" value="STH2401"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2401"/>
    <dbReference type="eggNOG" id="COG2256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017985_0_3_9"/>
    <dbReference type="OMA" id="ACEFVGM"/>
    <dbReference type="OrthoDB" id="977094at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="51"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="438" mass="48108" checksum="1DD290D4C64FB673" modified="2004-10-11" version="1">MDLFSWAAAQQAAQSAPLAARMRPQTLDEFVGQEHLVGPGRFLRRALEAGHLPSCILFGPPGTGKTTLARLMASNVNAHFEQLNAVTSGVQDIRRIVDEARARLAERGQKTVVFIDEIHRWRKDQQDALLPHVEDGLITLVGATTQNPLVSVNAPLVSRTRIFELKPLSDEHIAHLLDRALRTPGRGLGNYNATVTPEAMEHLVRMAGGDARAALNALELAVLLTQPDEQGRRTVTLETAEEALLKRAVVYDHDGDEHYHVASALIKSIRGSDPDAALYWLARMLYAGEDPRFIARRLVISAAEDIGLADPQALVIAQAAADAVSFIGMPEGRIPLAEAVVYLACAPKSNSAYLAIDRALAVVEREKTPPVPNHLKDTGYGSDQRLGYGKGYQYPHDFPGAWVEQRYLPEGVDGPFWEPKGYGREAELARLLRERRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU3</accession>
    <name>Q67LU3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196491.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2368"/>
    <dbReference type="ESTHER" id="symth-q67lu3">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41353">
        <property type="protein sequence ID" value="BAD41353"/>
        <property type="gene ID" value="STH2368"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2368"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_39_0_9"/>
    <dbReference type="OMA" id="HSMAWEN"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="28"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="267" mass="28487" checksum="8A14B5AA1F5E4A4E" modified="2004-10-11" version="1">MDLRLIRWPSRAGTLAVRMRAGGGPEPVLLLHGLGASHVFFLPAAAGKALAGRAVILPDLPGHGDSEDPAGFACAMDDLADLLMELLDWLGLSRVSLVGHSMGGTIAILMAERDPGRFTGLLCAEGNLRPEDATLSRRLAAMGAEGVRAALPRLVGKFAGSGLDGREDDLLYAATLSRCAPDVLWRYAAGLVRTSDTPGLLERFLSLPVPRTYVYGERTPGLEPLLESLRSHGVPTRMVPGAGHSMMVERPDVFDRILREHLAPKGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX2</accession>
    <name>Q67LX2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glucose kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2339"/>
    <dbReference type="EnsemblBacteria" id="BAD41324">
        <property type="protein sequence ID" value="BAD41324"/>
        <property type="gene ID" value="STH2339"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2339"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_0_1_9"/>
    <dbReference type="OMA" id="VDIGRGW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="33768" checksum="4F62ABFDD2C4C4D7" modified="2004-10-11" version="1">MYDDVVLGIDLGGTHLRLGLVDRNYQVSQFEIRKTREILQGDRPVQRLIDTVSAYIAAHLGDRLPAAVAVGFPSTVDRTRRVVMSTPNIPGLNDLPVADLMEQALGVPVFVNRDVNFLMLHDLLAHDLEGLPIVLGFYVGTGFGNAVFLDGRLLTGRHGMAAELGHVPLFRLQDRCGCGNPGCAEIIASGLRLERIQQEQFPDVPLEELFIRHASHPALRQFVEDLSLPIATEINIFDPDCIILGGGILHMEGFPRAELEAAIYRHARKPYPGLDFRIIYAAPNQESGVIGAGIYAYKRLQNPSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M37</accession>
    <name>Q67M37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2273"/>
    <dbReference type="EnsemblBacteria" id="BAD41258">
        <property type="protein sequence ID" value="BAD41258"/>
        <property type="gene ID" value="STH2273"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2273"/>
    <dbReference type="eggNOG" id="ENOG5032Y52">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_650401_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269263" evidence="1">
        <location>
            <begin position="26"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="422" mass="46736" checksum="F7D536C13C0B1C0D" modified="2004-10-11" version="1">MKIRGISAVLGVLMVVQLLVVPAYGAEVQDSSARLYTTEDRLHSGSGTAISARDPLVQVNPGGIALEGSITIDGLEVPYRITGNITRSAVNTDRLVVEATDTLDNYEVVFLALEQGEAVAPFFTDSAPETVAMLRLYMRDKRTDTFVVQELSSPLITKAIKEAWRFSADAPVNTVEAELWHAKVYEPVTVEEIDVTPVQATNFRTYDRTILYRVTYQISGKTVYEDFRVRHYLQCPTVITIQEECGAVLEIVEDRSWSPNDPNFGGPATFTEMREAEINFATDAGDYIQSAYWGGAWKNKGSVNFSVRLGYSFKLPLLPLSLSFSTGYKSSEQVASEGSKRYTVPNSNGEYVRNHGIAYPTTAILNDADYGHNIWAEVLVGHYTQPGQKQLMAGWKYTLINMGNTTWASTETVTGSVVYSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M94</accession>
    <name>Q67M94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2214"/>
    <dbReference type="EnsemblBacteria" id="BAD41199">
        <property type="protein sequence ID" value="BAD41199"/>
        <property type="gene ID" value="STH2214"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2214"/>
    <dbReference type="eggNOG" id="COG2433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688726_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269440" evidence="1">
        <location>
            <begin position="33"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="298"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="48207" checksum="E894DA16B2147608" modified="2004-10-11" version="1">MRRMMGQLRRRAIAWTWAVLTIMLLASPTAVGETEQKPEVAEYPAQNYDPGPGWPDGWSPPPIPAQMSERMRAEIDQVLQERADEFDGFEWWVWDPLPAWTQEYPWYWEEDWVPCGRTIYVYAYPKPDTQLIRQVYGRSFGWSRLYWKPCRYEPINSITEARGLSEEEALAEGRRAEYLYWVDKSHPGLGRDDGSSSNGNPDYIYVFLNQGNAWKRFDAPAYLDTTVNRVRVPIRFVSEMMGGQVLWDETGRRVTIHFPAVTRDVMKVIPTEGYDYTDLFDYEEYLPNGHRFYFEERTVSTPQRTIVLTIDHPVAVVDGQEVPLDAPPVIRNDRTMVPVRFIAEQMGAKVYWVGAQPIFRQDDGTMSGTYRPHLHAVLPPVRVPELVLRKPGCSVLRGIDRCTGDAGSQGHLRFSWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME0</accession>
    <name>Q67ME0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2168"/>
    <dbReference type="EnsemblBacteria" id="BAD41153">
        <property type="protein sequence ID" value="BAD41153"/>
        <property type="gene ID" value="STH2168"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2168"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_0_0_9"/>
    <dbReference type="OMA" id="YESITMQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="35"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="27269" checksum="90186BE9CEBE9E68" modified="2004-10-11" version="1">MTLLLLTLFGYTGLPTLLARTGFRRALTGLPAVPPQVALTFDDGPDPVWTPRILEILDRFGVRATFFMLGSRAEAHPDLVRQVAAAGHEVGVHGYRHRPAALQGLRSTLADVEATVRVLTGILQRRPRYYRPPWGIFNLWTGIAAKRHRLIPVVWSLHVAEWSRATTAHSIYLDAMRRAKPGTVLLLHDAAGPGSRPDAPAVVARALPRVIAGLRRRGLTPVSLSELLEPAERRVTDAPPGPDMGSPRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ3</accession>
    <name>LEUD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuD</name>
        <name type="ordered locus">STH2115</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LeuD family. LeuD type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD41100" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41100.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_043713907.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MJ3"/>
    <dbReference type="SMR" id="Q67MJ3"/>
    <dbReference type="STRING" id="292459.STH2115"/>
    <dbReference type="EnsemblBacteria" id="BAD41100">
        <property type="protein sequence ID" value="BAD41100"/>
        <property type="gene ID" value="STH2115"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2115"/>
    <dbReference type="eggNOG" id="COG0066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_0_3_9"/>
    <dbReference type="OMA" id="AFTTHTG"/>
    <dbReference type="OrthoDB" id="1384217at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141899" description="3-isopropylmalate dehydratase small subunit">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="203" mass="23159" checksum="7A09708FC185DC1E" modified="2006-01-10" version="2">MRPFTRETGLAVPLDRVNVDTDQIIPKQFLKRIERTGFGQFLFHDWRYLPDGSPNPEFVLNRPQYAGATILIAGRNFGSGSSREHAPWALSDYGFRAIIAPSFADIFYNNCFQNGLLPVVLPEEAVAELMRRAQEPGYRLTVDLERCVVEDDAGFRVDFAIDAFRRHRMLHGLDDIGLTLQYEDEIAAYEARRPAWLPTTPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ6</accession>
    <name>Q67MJ6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyoxalase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41097">
        <property type="protein sequence ID" value="BAD41097"/>
        <property type="gene ID" value="STH2112"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2112"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979716_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyoxalase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="31027" checksum="3503D40166994F65" modified="2004-10-11" version="1">MLSSPCRESPGAGFGGVMCTGASRVRILLPPYGVVGGDEENGRMAAVARKPAKVIPVRNLAESLAFYVDTLGCKLVAIHPDAAHLETPVGHVLLAGPDAGDVGRYAAPSYKVLKPGAVIYVFTPDIEETARRLPGAARIRTEWGERKLELADPNGYTISYWTESDLTDGQLLALFASAPERLEAALAGLRYAIAEPGRLYRPNAYDPDVWDAGPCSDRRPVQPSVALLRAVHGHLLQLIAHVPGALDRYTVNEAGSRQTVRRMVWMLGAHAAGHVEQIWMTRRVHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ9</accession>
    <name>Q67MQ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron-containing alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196175.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2049"/>
    <dbReference type="EnsemblBacteria" id="BAD41034">
        <property type="protein sequence ID" value="BAD41034"/>
        <property type="gene ID" value="STH2049"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2049"/>
    <dbReference type="eggNOG" id="COG1454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_0_9"/>
    <dbReference type="OMA" id="NAIPVHN"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="11"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="394" mass="42449" checksum="7854AAE6E25E2CAD" modified="2004-10-11" version="1">MLPRYYEFYNPVKVLSGEHALENLPYEMAHLHAKRPILLTNQQLVEVGLTRILQDALRGSDLTIAAQYTEIPQDSSIHVVNAAGRVFREAGCDSIIALGGGSVIDTAKGLRVLIGQETDDIMQYMGADILQPKRRVPLAVVPTTAGTGSEATLVAVIAHPERQVKMEFVSHHLLPDLAVLDPRMTRSLPPRITASTGMDALVHAIEGYTSIQRNPLSDAYAWAAIELIREYLPRAVANGQDTEARLAMANAALMAGAAFSNAMVGLVHAIGHAVGGVARVAHGDAMAILLPHVMEYNLDMLSDRYGRLLLALAGPEVYAATPDNVRGSQAIAVVRAFAERLHQACGLPLRLRDVGVTEAQLPAIARTTMNDGALLMNAKEAGPDDVMQILRKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY6</accession>
    <name>Q67MY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Stage III sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">spoIIIE</name>
        <name evidence="6" type="ordered locus">STH1972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1972"/>
    <dbReference type="EnsemblBacteria" id="BAD40957">
        <property type="protein sequence ID" value="BAD40957"/>
        <property type="gene ID" value="STH1972"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1972"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_292198_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsK" evidence="5">
        <location>
            <begin position="677"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="296"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="931"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="694"/>
            <end position="701"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1043" mass="106431" checksum="A70CA52B278F91F4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N09</accession>
    <name>AROK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate kinase</fullName>
            <shortName evidence="1">SK</shortName>
            <ecNumber evidence="1">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroK</name>
        <name type="ordered locus">STH1949</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N09"/>
    <dbReference type="SMR" id="Q67N09"/>
    <dbReference type="STRING" id="292459.STH1949"/>
    <dbReference type="EnsemblBacteria" id="BAD40934">
        <property type="protein sequence ID" value="BAD40934"/>
        <property type="gene ID" value="STH1949"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1949"/>
    <dbReference type="eggNOG" id="COG0703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057607_2_2_9"/>
    <dbReference type="OMA" id="DTDIFMQ"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000237941" description="Shikimate kinase">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <sequence length="174" mass="19043" checksum="64E4EFD0007243A0" modified="2004-10-11" version="1">MNIVLVGLMGSGKTAVGRLLAERLGRPFVDTDRLVEADAGRTVADIFAAEGEEGFRRREAEVVARAAAGDNQVIATGGGAVLRTENREALRRTGFVIWLDAEPETLYDRARGQGLHRRPLLSGPDPLGRLRALAAARRPFYAQAAHVRICTDRRSLQDVVAEIMEKLQERGERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N60</accession>
    <name>Q67N60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196025.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1898"/>
    <dbReference type="EnsemblBacteria" id="BAD40883">
        <property type="protein sequence ID" value="BAD40883"/>
        <property type="gene ID" value="STH1898"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1898"/>
    <dbReference type="HOGENOM" id="CLU_1659840_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="17825" checksum="940A14940C4FF4C5" modified="2004-10-11" version="1">MSRWQRALPLLASFAMGFAVAIWLLPVMAGPTVQRLRLERDFCLARVETLEAEVARLEQAERRRPVVCQVARVRAEIEGPDERVALEAARRIQKELAAEQVGRQLENVSPVILYGRYHGRLLEIDGITYQLSVQVLLIGPELALLGRVEPVEKGTEGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N93</accession>
    <name>Q67N93_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195993.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1865"/>
    <dbReference type="MEROPS" id="M24.008"/>
    <dbReference type="EnsemblBacteria" id="BAD40850">
        <property type="protein sequence ID" value="BAD40850"/>
        <property type="gene ID" value="STH1865"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1865"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_0_9"/>
    <dbReference type="OMA" id="HNTQYLL"/>
    <dbReference type="OrthoDB" id="415910at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="2">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="1">
        <location>
            <begin position="138"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="357" mass="39194" checksum="43A1576729876460" modified="2004-10-11" version="1">MSQARLARLRARLEERGLDAVLIAKPHNRAYLSGFTGSAGLLLITADRAALVTDFRYVEQAAAQAPEFEVIKAESANQAVLTRLVEEWGVKRIGFEGDFLTVDEHQTYQQFLAGCQWTSVSGLVEELRMIKDETEIALMRRAAEIADEAFAQILPLIKPGVIERDLATELEYRMKKLGAEGVAFETIVASGARSSLPHGVASDKAIEVGDLITFDFGAVYQGYCSDMTRTVMLGEPTDKQREIYGIVLEAQKRGVAACRPGITGRELDDVCRSYIAEKGYREYFGHGTGHGVGRYIHEGPRVSQRGGDVVLRPGMVVTVEPGIYLPGWGGVRIEDMLLVTESGAESFTHSPKELLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB9</accession>
    <name>Q67NB9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1839</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1839"/>
    <dbReference type="EnsemblBacteria" id="BAD40824">
        <property type="protein sequence ID" value="BAD40824"/>
        <property type="gene ID" value="STH1839"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1839"/>
    <dbReference type="HOGENOM" id="CLU_3012645_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="56" mass="6005" checksum="E08C37B1F001D302" modified="2004-10-11" version="1">MMFYGFGKPFYGKSFLGKPFVGKPFFGKGFPATKASGFNSFIPNTTVATTPFPGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF2</accession>
    <name>Q67NF2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ordered locus">STH1806</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195934.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1806"/>
    <dbReference type="EnsemblBacteria" id="BAD40791">
        <property type="protein sequence ID" value="BAD40791"/>
        <property type="gene ID" value="STH1806"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1806"/>
    <dbReference type="eggNOG" id="COG0041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_2_9"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="1701464at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="9"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="17111" checksum="902A36E19AAA772C" modified="2004-10-11" version="1">MAQTERTGPLVGIIMGSRSDLETMQEAAAVLDELGIPYEMKVVSAHRTPDAMFAYAESAAARGLKVIIAGAGGAAHLPGMTASKTLLPVIGVPVKSSTLNGIDSLLSIVQMPGGVPVATMAIGKAGARNAGLLAAQILALEDPGLRAILEEYRAYMRQQVEESQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI3</accession>
    <name>Q67NI3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1775"/>
    <dbReference type="MEROPS" id="M19.007"/>
    <dbReference type="EnsemblBacteria" id="BAD40760">
        <property type="protein sequence ID" value="BAD40760"/>
        <property type="gene ID" value="STH1775"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1775"/>
    <dbReference type="eggNOG" id="COG2355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031404_2_1_9"/>
    <dbReference type="OMA" id="CDHPRNI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000180">
        <property type="entry name" value="Dipep_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="PTHR10443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00869">
        <property type="entry name" value="RENAL_DIPEPTIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="309" mass="33506" checksum="9531A063A23BFC9A" modified="2004-10-11" version="1">MIDGHADSILMVLAGERTLTERSDKGHIDWPRAREAGVTCTVQAAWVDPVYYPVAPRRAHEAIDGLLGQIDAAEGVRLVLRADDVTAAHAAGELAVLLNIEGAEAIQGSLALLRNYYRLGVRMMGLVWNHRNEVAEGVGEPESGGGLTRFGREVVREMNRLGMLIDLAHITPAGFFQVLELSEDPVLFTHGNCQALYDHPRNLTDEQIRALAAHGGVFGISFVSSFMGRETAALTTVADHIDHVCQLLGNADHVGLGSDFDGATPPPGLEDVTCLPRLTAELMRRGYGEADLVKILGGNYLRVFQQVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ5</accession>
    <name>Q67NJ5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF402 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195891.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1763"/>
    <dbReference type="EnsemblBacteria" id="BAD40748">
        <property type="protein sequence ID" value="BAD40748"/>
        <property type="gene ID" value="STH1763"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1763"/>
    <dbReference type="eggNOG" id="COG3557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1509187_0_0_9"/>
    <dbReference type="OMA" id="RQFPFDG"/>
    <dbReference type="OrthoDB" id="1769424at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016882">
        <property type="entry name" value="UPF0374"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028345">
        <property type="entry name" value="UCP028345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="SSF159234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF402" evidence="1">
        <location>
            <begin position="55"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="20525" checksum="0863FB6389E8B418" modified="2004-10-11" version="1">MWVQQYKYGGRKHYSYPVRLIEERSDVIIVHGAYGRPLTHPGRGLVDWPVPNESIEFHFTSAPCSVSAAWNADGSFRHYYGNVNTPATLSEGVLSLVDLDLDLLVTEDLAWRVEDEDEFEAHRREWGYPEEVVELARSALAELIQMVEERRFPFDGSAFRLREQLSRSGAPRAESARRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL5</accession>
    <name>Q67NL5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1743</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195871.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1743"/>
    <dbReference type="EnsemblBacteria" id="BAD40728">
        <property type="protein sequence ID" value="BAD40728"/>
        <property type="gene ID" value="STH1743"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1743"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32972" checksum="71EF4F2862CEA0B5" modified="2004-10-11" version="1">MNGNPLIDVQGVRKGFAGRPVLTELTFAVGRGEIVGFIGPNGSGKTTTVRLLNGVLAPDAGHIAVGGFDPGRDGDRVRRMSGILTESAGLYGSMTGRENLRFFAELYGVDEPGRVDELLAAFGLADAADRKVAAYSTGMRKRLGLAKAVLHRPEVLFLDEPTNGLDPEGIRMVLGYIRSLNEQEGTTVLICSHLLQQLELVCHRYLFLLGGQVVEQGTLAELEARHFPAVTLEVETDLALTDGRYRGVPARQVRPGRIAFTLPGREDVPGFLRILAQDAAVYSAVPVRRDLETLYFKIMGGEAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS6</accession>
    <name>Q67NS6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1682</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1682"/>
    <dbReference type="EnsemblBacteria" id="BAD40667">
        <property type="protein sequence ID" value="BAD40667"/>
        <property type="gene ID" value="STH1682"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1682"/>
    <dbReference type="eggNOG" id="COG1971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094526_0_0_9"/>
    <dbReference type="OMA" id="VGITYGM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003810">
        <property type="entry name" value="Mntp/YtaF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014205">
        <property type="entry name" value="Spore_YtaF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35529">
        <property type="entry name" value="PTHR35529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02659">
        <property type="entry name" value="Mntp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02840">
        <property type="entry name" value="spore_YtaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="21626" checksum="1B806CFEC79A44E1" modified="2004-10-11" version="1">MDSVSLVLLAAALSLDALMAGLLYGLRGIRVSPGAAAIVSGATALLLGAAMGAGAFLAARLPPAAAEQAGAAILAATGLWITAQTLRSHPGVRRRAGEPTPRRVWRLRLGSFGIVVEILREPSVADLDRSGHINPAEALLLGVALALDSVAAGLGAGMTGLSPLGLPLAAGVTSFALLSAGSRLAGHLPLRLGGRWAALHGVMLALLGLYRMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU7</accession>
    <name>Q67NU7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1661</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CPA3 antiporters (TC 2.A.63) subunit G family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1661"/>
    <dbReference type="EnsemblBacteria" id="BAD40646">
        <property type="protein sequence ID" value="BAD40646"/>
        <property type="gene ID" value="STH1661"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1661"/>
    <dbReference type="eggNOG" id="COG1320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121334_0_3_9"/>
    <dbReference type="OMA" id="MAITFAT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098662">
        <property type="term" value="P:inorganic cation transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005133">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34703">
        <property type="entry name" value="PTHR34703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03334">
        <property type="entry name" value="PhaG_MnhG_YufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01300">
        <property type="entry name" value="CPA3_mnhG_phaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="12497" checksum="A3D6AC94F244B73A" modified="2004-10-11" version="1">MATEILIGLLVLLGTAAAVLGTLGLVRMPDVYNRLHAVTMAITFATVMMVLAGTLYFSTTHGLTLKLLLVIPFVFWTSSAGSFVIARGAHRTGVRPAPLTIRDDLQEDIGIDQEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX3</accession>
    <name>Q67NX3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_M28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1635"/>
    <dbReference type="EnsemblBacteria" id="BAD40620">
        <property type="protein sequence ID" value="BAD40620"/>
        <property type="gene ID" value="STH1635"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1635"/>
    <dbReference type="eggNOG" id="COG2234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047405_0_0_9"/>
    <dbReference type="OMA" id="VNGANDN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M28" evidence="2">
        <location>
            <begin position="214"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="393" mass="43157" checksum="CB6E97CD8556D585" modified="2004-10-11" version="1">MKGDGDVNGAWTYLERLTRYPHRGVGSPEEAAAAEEAAGWLRDMGYDVEIQRFRAPRDTLYIGPAVIMLGYLAAGAIAAWWQPWAGLVLAVLLLIPLVGEMSGRGLDLDLYTPKVTSQNVIARRPERGGERLTVVVSGHLDTQHATWLFHPRMARHVQTYLNVAYGSLGLMFLGLLLRAVLPAAGWPLWILAAGAVLLALHIAFLLSCAFGGGYINGANDNTTGAALTLALAEHFAAHPLPGVRFHFVLTGAEEVGTRGMKAFLRAHRYDPDSTYFINLDNLGGGTLTYLEGEGMTLYHRFGKDLVEMARIMAAERPGRVKARPNLLLPTDAMIPAAHGYQAISFLAFLEDGSLPNYHWYSDTLEKVDRELLAFTEAFLKEYIQRLAVSRIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY2</accession>
    <name>Q67NY2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195755.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1626"/>
    <dbReference type="EnsemblBacteria" id="BAD40611">
        <property type="protein sequence ID" value="BAD40611"/>
        <property type="gene ID" value="STH1626"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1626"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_7_3_9"/>
    <dbReference type="OMA" id="YHVEHLR"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018334">
        <property type="entry name" value="ArsR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00846">
        <property type="entry name" value="HTH_ARSR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="13221" checksum="9227ADF699BD9EF1" modified="2004-10-11" version="1">MDDVCPRPDADEARIESLRERLLDVAGLSELFRVLADETRTKILYLLAAEELCVHDLARILDLTLPTISHHLRLMRLMRLVKSRREGKHVYYSLSDDHVLQLIQTAQEHYQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P05</accession>
    <name>Q67P05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ferrichrome ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195732.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1603"/>
    <dbReference type="EnsemblBacteria" id="BAD40588">
        <property type="protein sequence ID" value="BAD40588"/>
        <property type="gene ID" value="STH1603"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1603"/>
    <dbReference type="eggNOG" id="COG4604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_9"/>
    <dbReference type="OMA" id="RYAWNGL"/>
    <dbReference type="OrthoDB" id="1048863at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="28861" checksum="5D13113FD695715D" modified="2004-10-11" version="1">MVKVRGVSKSFGPKRVLHDVSLDIEKGKVTTLIGPNGAGKSTLLSLMSRLLPKDSGQILIDGREIGQYKSEELARRLAILKQTHHLNIRLTVRELVEFGRFPYSHGRLTREDREVVDEALRYLDLHEIEGKYLDQLSGGQRQRAFIAMVLAQDTEYVLLDEPLNNLDMKHAVQIMRITRSLANDLGKTVVLVLHDVNFASAYSDHIVALKDGRVAAAGTPDELIRSDVLGDIYEMKIPIETVGGCRVCIYFTGEQAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P18</accession>
    <name>Q67P18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase I subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nuoE1</name>
        <name evidence="8" type="ordered locus">STH1590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1590"/>
    <dbReference type="EnsemblBacteria" id="BAD40575">
        <property type="protein sequence ID" value="BAD40575"/>
        <property type="gene ID" value="STH1590"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1590"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1015370_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="28627" checksum="EF9C75BA06C4C7D6" modified="2004-10-11" version="1">MIEEVHPPRWPETCKDEVEAILRRYPEGRERSAILPLLHLAMREREGRYIAQSDIEAVAEICGVAPSYVQSVCSFYTMFRRQPVGKYLITVCGNMACHLLAGGSQLVEHMEKTLGIKVGETTPDGLITLEVTGECLAACDLAPVIHVDTEYVVKVTREKFDALVAALRAGEGPDRFLEKLPLMNGESQDEWPGFVDAGTPAPAAAGPAAEGSPDPSAPGAAAQAPPAESGGTAPPYQVVDGAARPSADGSAPDAPADGGAPQAADAGSGPAEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P19</accession>
    <name>NUOD1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit D 1</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit D 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit D 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoD1</name>
        <name type="ordered locus">STH1589</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P19"/>
    <dbReference type="SMR" id="Q67P19"/>
    <dbReference type="STRING" id="292459.STH1589"/>
    <dbReference type="EnsemblBacteria" id="BAD40574">
        <property type="protein sequence ID" value="BAD40574"/>
        <property type="gene ID" value="STH1589"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1589"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01962">
        <property type="entry name" value="NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000357943" description="NADH-quinone oxidoreductase subunit D 1">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01358"/>
        </source>
    </evidence>
    <sequence length="404" mass="45942" checksum="15CAE7C39A248B93" modified="2004-10-11" version="1">MSMAEDLHLEEQRPERMTLSIGPHHPATHGVLRVKLELEGETVVKAEPETGFLHTGIEKTAEHLTWNQATTVMDRMDYLSPISNNTGYVMAVEKLLGIEDRIPEKARVTRVILLELNRVASHLVGLGTGGLDYGNIGTPIFWAFELRDRILDIFEHTTGQRMNPSYMRVGGLAYDLPHNFKEMVEDFLKVAPGRIQELKDVMLHNPIFVDRAKGVSVITYEQALRYGLTGRNLRVTGSDYDVRKYYPYLGYENYDFKVPVYTDGDSWSRVAITFDEMFESLKIIRQALDNLPWDDRYIIDDRKLVLPPKQEVKHSMEALIHHFKLVHHGFDVPAGEVYVSIESPRGEIGFYVVSDGGNKPMRVRVRPPSFYAVYSLPVLLEGHLLSDMVGAIATIDPVFGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P28</accession>
    <name>Q67P28_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1580"/>
    <dbReference type="EnsemblBacteria" id="BAD40565">
        <property type="protein sequence ID" value="BAD40565"/>
        <property type="gene ID" value="STH1580"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1580"/>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_717515_0_0_9"/>
    <dbReference type="OMA" id="GMNEARI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025645">
        <property type="entry name" value="DUF4349"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14257">
        <property type="entry name" value="DUF4349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="309"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="385" mass="40218" checksum="19C9F2769348FBA4" modified="2004-10-11" version="1">MDPGAAAAGGSGTGPLRGPSIGQDACAHREGLHRMNDRKRTVWLALLVVLSLILGGCGGAPQSAARDSGGPGGRSGSASSPSPAPAAEVPAGTPWAGSVSFLDTAENPGDEAAAPTADRKIIQNAEVELSVRDVDEALTSITDALTRSGGYVQENRVTGTREQGRRVAMTLRVPAGNFGPLLDLLRELGEVQSLRQWTNDVTEEYLDLEARIETGEAHLAQLNRLYERSGTISEMIELEREIARVTAELESLKGRYNFLANQVAFSTVSVFMYEPGVPAPARSPQSLGERMRDSFLFSWNATIHFAEGLLVALVGLIPGLLFLAVLVGIVGGVIWLIVRVRRGRGGPPGGGSRGEPAARPLYSPPGADTAGDRPGGGAGAGQAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P45</accession>
    <name>Q67P45_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40548">
        <property type="protein sequence ID" value="BAD40548"/>
        <property type="gene ID" value="STH1563"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1563"/>
    <dbReference type="HOGENOM" id="CLU_1834162_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="15745" checksum="F7DBD3B460C93E20" modified="2004-10-11" version="1">MPGCRHHLLRRLGSAAIPSQARTCPARNSRRTRHRGVAPGSYAEPSKRPKRHPCAASPNRRQQKGGRVVRKRTRGKWLLLSLTAVTAVVFLAHCSPSREAEVAARWTTDDVQRITIDVPRPCRPLSVDEFVCDEVILRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P78</accession>
    <name>Q67P78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1530"/>
    <dbReference type="EnsemblBacteria" id="BAD40515">
        <property type="protein sequence ID" value="BAD40515"/>
        <property type="gene ID" value="STH1530"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1530"/>
    <dbReference type="HOGENOM" id="CLU_1712344_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="16465" checksum="35B7E16A09C7617E" modified="2004-10-11" version="1">MRQRVEEAMAEGVLDLPANLVAHVERCPHCSAEVREVEMLLHRLRSLPASLDLSPVPAAVDRVLQATASNLTAAGAVPAPAPVEKKRRRTPQWQWVLGQVAAVAAVIAIAAGGLTLLGLGIHGAVSGQEPGRIVERWVAPLRDWTQALFRNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH5</accession>
    <name>Q67PH5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Germination protease</fullName>
            <ecNumber evidence="1">3.4.24.78</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GPR endopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Germination proteinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Spore protease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpr</name>
        <name evidence="2" type="ordered locus">STH1433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Initiates the rapid degradation of small, acid-soluble proteins during spore germination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase action with P4 Glu or Asp, P1 preferably Glu &gt; Asp, P1' hydrophobic and P2' Ala.</text>
            <dbReference type="EC" id="3.4.24.78"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A25 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.78" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195563.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1433"/>
    <dbReference type="MEROPS" id="A25.001"/>
    <dbReference type="EnsemblBacteria" id="BAD40418">
        <property type="protein sequence ID" value="BAD40418"/>
        <property type="gene ID" value="STH1433"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1433"/>
    <dbReference type="eggNOG" id="COG0680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055087_1_0_9"/>
    <dbReference type="OMA" id="PMGNYIT"/>
    <dbReference type="OrthoDB" id="799376at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00626">
        <property type="entry name" value="Germination_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005080">
        <property type="entry name" value="Peptidase_A25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03418">
        <property type="entry name" value="Peptidase_A25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019549">
        <property type="entry name" value="Peptidase_A25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01441">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005078650" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023497773" description="Germination protease" evidence="1">
        <location>
            <begin position="13"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="34626" checksum="5E5A217922D5E377" modified="2004-10-11" version="1">MQARDYLSPFTDLALEATAAARGDARTELPGVVMREERYDVATVSWVEVMPGVGEQTIGKPAGNYVTIDAPQLRRRNRDLQEQVGKILVEQLNKLLHLKEDDTVLVVGLGNWNATPDALGPRVTGKLMVTRHLREFVPADVAGGLRAVAAIAPGVLGTTGIETAEVILGIVERIKPAVVICIDALAARSVERIGTTIQIADTGIAPGGGIGNKRQALNRESLGVDVIAIGVPTVVHATTIVQDAFEAMAQSFAGTKPFFQLFNSREERQKLLSEVLSPTVGELVVTPKEIDELTEDMAKLIAGSLNAVLHTSVTAQDLLRYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL8</accession>
    <name>Q67PL8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DJ-1_PfpI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195520.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1390"/>
    <dbReference type="MEROPS" id="C56.A06"/>
    <dbReference type="EnsemblBacteria" id="BAD40375">
        <property type="protein sequence ID" value="BAD40375"/>
        <property type="gene ID" value="STH1390"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1390"/>
    <dbReference type="eggNOG" id="COG0693">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_9"/>
    <dbReference type="OMA" id="YTWPADL"/>
    <dbReference type="OrthoDB" id="1421548at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42733">
        <property type="entry name" value="PTHR42733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="2">
        <location>
            <begin position="4"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="20876" checksum="62F5219928A89D91" modified="2004-10-11" version="1">MSAKKILMIVGDFVEDYEVMVPFQALQAVGHTVHAVCPGKKPGEKVRTAIHDFEGDQTYSEKPGHNFAVNADFDEVNPEDYDALVIPGGRAPEYLRLNDKVVAMVRHFGEANKPIAAICHGAQLLAAADVLKGKACSAYYACAPEVKLAGAEYVHLEADQAHTDGNLVTAPAWPAHPAWIAQFLKVLGTKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM7</accession>
    <name>Q67PM7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1381</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1381"/>
    <dbReference type="EnsemblBacteria" id="BAD40366">
        <property type="protein sequence ID" value="BAD40366"/>
        <property type="gene ID" value="STH1381"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1381"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080722_0_0_9"/>
    <dbReference type="OMA" id="WEYRDRE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="102"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="28236" checksum="53805861222868A6" modified="2004-10-11" version="1">MVHLRETYRAKPGRTDALARVLSDRLIPACVKHGAALIGWWVAPADGEMVIIWDCPQLAEHRRIIAEARRDPLWPEDGWTDELVETRREELLEPMDRYGPPRHTLAVSGFIADGEGRVLLVRTRLRSDTWELPGGQVEAGEDPVTALVREIREETGIEAEIQGLTGVYYSTARGRVCNLVFRGVAVGGRLSSSPETLEAAFVQLRADTVSQWVTRPHFQVRVTDALGAHTLPYETYRLRPYARLLRLSGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN8</accession>
    <name>Q67PN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative UDP-glucose 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195500.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1370"/>
    <dbReference type="EnsemblBacteria" id="BAD40355">
        <property type="protein sequence ID" value="BAD40355"/>
        <property type="gene ID" value="STH1370"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1370"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_9"/>
    <dbReference type="OMA" id="LGIRACY"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05257">
        <property type="entry name" value="Arna_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045869">
        <property type="entry name" value="Arna-like_SDR_e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="10"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="36221" checksum="0F983ECC9799C9A0" modified="2004-10-11" version="1">MGEWQSKKVLVTGGTGFIGSHVVQALLAEGARVRVLAHYNGGGHLGNLAELAPQERAEVEVVWGDLRDPDSVRRAVQGMERVLHLGALISIPYSYLDPRSYVDVNVTGTLNVLLACRDLGVERLVHTSTSEVYGTPDTVPIAETHPLRGQSPYAASKIGADKLAESFHRSYGLPVVTLRPFNAYGPRQSTRALIPTILTQALWAPEIRLGSLWPRRDYTYVTDTAQAFLRAGSAPGVEGMVLNAGYGADVSVQELVEMALRLTGRRVPVVEAAERVRPAASEVARLLCDRTLAERRLGWRPQVTLEEGLRRTLAFIERRFTPDILKQTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP0</accession>
    <name>Q67PP0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1368</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1368"/>
    <dbReference type="EnsemblBacteria" id="BAD40353">
        <property type="protein sequence ID" value="BAD40353"/>
        <property type="gene ID" value="STH1368"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1368"/>
    <dbReference type="eggNOG" id="COG0677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_3_2_9"/>
    <dbReference type="OMA" id="IDVWLMA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="4">
        <location>
            <begin position="272"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="357" mass="38349" checksum="BA34FED3DF41D229" modified="2004-10-11" version="1">MKVCVVGVGNIGTNLLTSLRRKGVQAVGVEINPDRRAALAAAGVDGVFAGPDEAGAPDVWILTTSTGPGMSHILSAVRSLSPKPGALVSVESTLPVGGTARLAEAFRERGYVPGETFHLAHVPHRILFGVEETVFDAPRVVAGVTPACRDAAIAFYRPLVPVLYPVDDVRVAELSKIVENGLRYLNIAFAEALFGYCLEHGLDWAQLHGAVNTKPNVELLNVDFGIGGECLPKDMGFLQEALQSPLLEAAVAADEAHRARLRRLAGPHRRVLVMGLTFKPGFPDTRFSRARELAEALAADGKEVYVYDPALGPEKVTELGFRWGDPGGAYDLIYQRPLAIRTAAEEEKRGQDTRDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP1</accession>
    <name>Q67PP1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycos_transf_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195497.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1367"/>
    <dbReference type="EnsemblBacteria" id="BAD40352">
        <property type="protein sequence ID" value="BAD40352"/>
        <property type="gene ID" value="STH1367"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1367"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068877_0_0_9"/>
    <dbReference type="OMA" id="NGGNRDY"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="224"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="38090" checksum="C185C8CF6840AA43" modified="2004-10-11" version="1">MRVVIPVLHLHRGGGCRVIAEAANQLVERGHDVTVVVLEGLPLAYPLKSRVKRVSKITPSVIPEADVILPNFWPTVRPAAESGKGRVVRFSTGFEPMWVPENKAALDTYLQGFPVITLGPHLRRIIREATGQDCFIAQPGVDGRVFRPLDGAPRDGRTVFFIYRSEAHGYHYKGNGDFAAAMELVRRQMPDVRVLVAATEGDSPGTPPPPFAFPFTEIRPEDDRAMAEAYNRAAVYVLASWFEALSLPPLEAMACGTPVVLTDCGGVRDYARPGENCLLSPPKQPDRLAGYILSVLREPDLRSRLAEGGLRTAAVWTWKRFGDQLEDALRQIMQGHVQPQEARDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT3</accession>
    <name>Q67PT3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA_edit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1325"/>
    <dbReference type="EnsemblBacteria" id="BAD40310">
        <property type="protein sequence ID" value="BAD40310"/>
        <property type="gene ID" value="STH1325"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1325"/>
    <dbReference type="eggNOG" id="COG2606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094875_0_0_9"/>
    <dbReference type="OMA" id="PLPIYCD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="1">
        <location>
            <begin position="13"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="15041" checksum="D4D08BBA7FA16604" modified="2004-10-11" version="1">MEGEIRLFGKLLTTAQAAADALGVDLGRIAKSVLMFAGGEPVLVVAAGDRRVDRRKVRDRLNRGKVTLAGPEEVLAVTGFVAGGVAPVGALHPVTVLLDESLRRFPDIWAGGGVPEALLRIRVDDLPRATGGVFADVVQDPAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU7</accession>
    <name>Q67PU7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sugar ABC transporter sugar-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195442.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1311"/>
    <dbReference type="EnsemblBacteria" id="BAD40296">
        <property type="protein sequence ID" value="BAD40296"/>
        <property type="gene ID" value="STH1311"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1311"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_10_5_9"/>
    <dbReference type="OMA" id="FMGGAAD"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01547">
        <property type="entry name" value="SBP_bac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269317" evidence="2">
        <location>
            <begin position="25"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="435" mass="48595" checksum="E9558312BE83E2F6" modified="2004-10-11" version="1">MRRRTHTLLTAMLLTFTLLVTACGSTQTDNSAQQTPQPQSQEQTDQQAASREKVTIRLATWAGAQEAQELEAIIDRINAESETFQIVHEPAPADYYTKIQTMLAGGTAPDLIWLAQEYVVPYAARGALMDLTDRLKADSRPAANLDDYFPLVLEHAMYEGKVYGLPWIGQPVIVYYNKDLFDEKGIPYPTDDWTWEEFRNLAVQLTDKEKGIYGTSFNGWPPVHMFIWQAGGEVISPDLKSSPIDSPEAIQALEFYSSIIYNEEVAPSMATVNEQGFSEMFKAGKIAMFFGGASDDLDRVEGLRVGHVRVPKGPANRTTFAWAASTVINAKTPHPDEAYDALIALTEGIHNWKIVPPRRSMATAEQITKNEPRKAEGAEVIMQAAQEMRAFRIIPNHPEWDTIFWEQLQDPLYSKKGSAAELAARARASLEEVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q09</accession>
    <name>Q67Q09_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PTS system N-acetylgalactosamine-specific IIB component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">agaB</name>
        <name evidence="9" type="ordered locus">STH1249</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195380.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1249"/>
    <dbReference type="EnsemblBacteria" id="BAD40234">
        <property type="protein sequence ID" value="BAD40234"/>
        <property type="gene ID" value="STH1249"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1249"/>
    <dbReference type="eggNOG" id="COG3444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116175_2_1_9"/>
    <dbReference type="OMA" id="VGNMHFH"/>
    <dbReference type="OrthoDB" id="1696140at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00001">
        <property type="entry name" value="PTS_IIB_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.35.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004720">
        <property type="entry name" value="PTS_IIB_sorbose-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036667">
        <property type="entry name" value="PTS_IIB_sorbose-sp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018455">
        <property type="entry name" value="PTS_IIB_sorbose-sp_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03830">
        <property type="entry name" value="PTSIIB_sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52728">
        <property type="entry name" value="SSF52728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00854">
        <property type="entry name" value="pts-sorbose"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51101">
        <property type="entry name" value="PTS_EIIB_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIB type-4" evidence="8">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="158" mass="17506" checksum="3C40796E8331041D" modified="2004-10-11" version="1">MPNILLTRIDNRLIHGQVATQWCSSIGANLILVANDQVAHDKMRQGLMDMAAPAGVETRYFTIEKTINVIHKASPRQKIFIICETPQDVLRLVEGGVPIKKVNIGNMHMAEGKRQVATSVAVDDADVAAFRRLQELGVELEIQRVPSTPPEDIAKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q33</accession>
    <name>Q67Q33_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Purine nucleoside phosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1225</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosine + phosphate = adenine + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27642"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:27643"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27647"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:11853"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="5 9">Belongs to the purine nucleoside phosphorylase YfiH/LACC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195356.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1225"/>
    <dbReference type="EnsemblBacteria" id="BAD40210">
        <property type="protein sequence ID" value="BAD40210"/>
        <property type="gene ID" value="STH1225"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1225"/>
    <dbReference type="eggNOG" id="COG1496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065784_0_0_9"/>
    <dbReference type="OMA" id="CFADCVP"/>
    <dbReference type="OrthoDB" id="1393323at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16833">
        <property type="entry name" value="YfiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003730">
        <property type="entry name" value="Cu_polyphenol_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038371">
        <property type="entry name" value="Cu_polyphenol_OxRdtase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30616">
        <property type="entry name" value="PTHR30616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02578">
        <property type="entry name" value="Cu-oxidase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="SSF64438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00726">
        <property type="entry name" value="TIGR00726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="31512" checksum="6F28723E1C240589" modified="2004-10-11" version="1">MSVATWAEHDGVPVLHLTGLEALGGVRAIFTGRRGGVSQRWGGGLNWSLSVGDRPEDVRENRRRTLAILGLAPGSAFMGGLVHGNRVVAVRADGCDPTDDSRNRADDVRVIPGADGLITDRQGIALVITAADCVPVYLYDPVRRVIGVVHAGWRGTVAGVAAEAVRAMREHYGCDPADIHAAIGPSIGPCCYEVDEAVAGPVRQHFGDGAEGLLRPSARAGRHMLDLWAANRLDLTSAGVGQVHVAGACTACQRDRFFSHRAEAGGAGRGAAIILLSQSVKGGSPWAPSRSCGQSPGDAADGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q72</accession>
    <name>Q67Q72_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylmalonyl-CoA mutase, N-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1186"/>
    <dbReference type="EnsemblBacteria" id="BAD40171">
        <property type="protein sequence ID" value="BAD40171"/>
        <property type="gene ID" value="STH1186"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1186"/>
    <dbReference type="eggNOG" id="COG1884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_9"/>
    <dbReference type="OMA" id="IAQKEWV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="METMALONYL_COA_MUTASE" evidence="1">
        <location>
            <begin position="35"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="556" mass="61661" checksum="14FB084F5DF3BF16" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB7</accession>
    <name>Q67QB7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195272.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1141"/>
    <dbReference type="EnsemblBacteria" id="BAD40126">
        <property type="protein sequence ID" value="BAD40126"/>
        <property type="gene ID" value="STH1141"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1141"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051806_4_1_9"/>
    <dbReference type="OMA" id="WNLAEGG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="301" mass="31460" checksum="E3DAA78779B1CCCB" modified="2004-10-11" version="1">MRNGPLPRSGRRPRRRLHPGLSGFFSLPLTLVLTASGLALWTGCLVLAGLSPDEALGRLNVLTIYVAMTLAALGSTWLCSRFLDGEPRPDYGLPLAPLPLAHGLAGIAAGVAAQGALYPAGLLTTVYAARWNAGPGPAFWHYALTQFFVAVGEELFHRGYLLRRWTAWLGRWPAAAATSLLFGAAHLLSPGAGILSTVGIAAHGLVYAWIVQATGSLWPAVGLHWAWNVMEAGVLGLPNSGTPAPFALVATAVNGPAWWTGGDFGPEAGVISLVAIPVAWLVLWPAARGAYRPSGAGTAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC5</accession>
    <name>Q67QC5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1133"/>
    <dbReference type="EnsemblBacteria" id="BAD40118">
        <property type="protein sequence ID" value="BAD40118"/>
        <property type="gene ID" value="STH1133"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1133"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1026467_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="27722" checksum="8FC9DBBC0B6923F3" modified="2004-10-11" version="1">MTFETFPQSTETIIDQVDNVKLHVRSHQSYTEFWSESDSVLLVEGRGWSPVLAADGSVIFVDSEDLSLKKLLINGDEQQLLPPGSAAGFLRISSDRKYLGYAIPINLSPDSNWAGLYGAGIMDLETGKVLVSRTKENFFTSPIGWYGDQLIVHEWDATQTQDSLDLYALSLDGELTKFALGLPQANSYPEISPDHQWLVYEDQDGNTILVNLEQVSFGIISNVVLPQWTSEGLTGLVDGQRFLIRFTIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD6</accession>
    <name>SYY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">STH1122</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195254.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QD6"/>
    <dbReference type="SMR" id="Q67QD6"/>
    <dbReference type="STRING" id="292459.STH1122"/>
    <dbReference type="EnsemblBacteria" id="BAD40107">
        <property type="protein sequence ID" value="BAD40107"/>
        <property type="gene ID" value="STH1122"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1122"/>
    <dbReference type="eggNOG" id="COG0162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024003_5_0_9"/>
    <dbReference type="OMA" id="VHPRDAK"/>
    <dbReference type="OrthoDB" id="402899at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02007">
        <property type="entry name" value="Tyr_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024108">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="PTHR11766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF1">
        <property type="entry name" value="PTHR11766:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000236762" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="346"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="234"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02007"/>
        </source>
    </evidence>
    <sequence length="408" mass="45544" checksum="132136E8B58E5DFE" modified="2004-10-11" version="1">MTMAFMSVDEQMKILMRGVVDLVSEEELRQKLERSVKTGRPLRVKLGIDPTGKDLTLGHTVPLRKLRDFVECGHQGVLIIGDYTAMVGDPTGRNEARPQLTHAETTANAQRYLEQAARVLDVSKLEIRRNSEWLAPMSFSDVIRLAAKSTVARMLEREDFKKRYEEGRPIFIHEFFYPLMQGTDSVAVQADVELGGTDQKFNLLAGRDLQRDAGQEPQVCLMTPIVEGLDGVQKMSKSLGNYIGLDHTPDEMFGRTMSIPDSLIITYFTYFTDVPQEEIERIEAAMAAGENPMTFKKQLGRAIITTYGGTEEEARLAEERWVAQFSRGEVPEDIPDVVLPAAELPMQAARVLFTAGLAPSLSEARRLIEQGGFTVDGEKVTDPRAELALRPGQVLKAGKRKYGRVVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE6</accession>
    <name>Q67QE6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1112</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195244.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1112"/>
    <dbReference type="EnsemblBacteria" id="BAD40097">
        <property type="protein sequence ID" value="BAD40097"/>
        <property type="gene ID" value="STH1112"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1112"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_9"/>
    <dbReference type="OMA" id="AQADKMR"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032524">
        <property type="entry name" value="ABC_tran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16326">
        <property type="entry name" value="ABC_tran_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="328"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="550"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="360"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="646" mass="72932" checksum="52C38C97EA3D04B3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL7</accession>
    <name>Q67QL7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1041"/>
    <dbReference type="EnsemblBacteria" id="BAD40026">
        <property type="protein sequence ID" value="BAD40026"/>
        <property type="gene ID" value="STH1041"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1041"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="CKEAAND"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="9"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="135"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="135"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="26360" checksum="1B4209C0050EC623" modified="2004-10-11" version="1">MAMATGKKRILVVDDDPKILKALDQALQQEGYEVYRAEDGLQALEMARKVKPDLMILDIMLPKMDGFEVLAQLQSTGGVPTLILSARGEEMDKVVGFNVGADDYLVKPFRLSELLLRVRAILRRTAGAGAPVDEDRPLKFRDIEINRSSRTVIVRGQPVDLTPKEFDLLWLLASHPGHVFSREALLQRVWHSEYSGDEAALTVCVRRLREKIERDPGHPELIKTIWGIGYKFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL8</accession>
    <name>Q67QL8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1040</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1040"/>
    <dbReference type="EnsemblBacteria" id="BAD40025">
        <property type="protein sequence ID" value="BAD40025"/>
        <property type="gene ID" value="STH1040"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1040"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_64_9"/>
    <dbReference type="OMA" id="ENCLECH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021796">
        <property type="entry name" value="DUF3365"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11845">
        <property type="entry name" value="DUF3365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="216"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="305"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="257"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="521" mass="58069" checksum="EF1C16E6A63E4E8D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ6</accession>
    <name>Q67QQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">5'-nucleotidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1002"/>
    <dbReference type="EnsemblBacteria" id="BAD39987">
        <property type="protein sequence ID" value="BAD39987"/>
        <property type="gene ID" value="STH1002"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1002"/>
    <dbReference type="eggNOG" id="COG0737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005854_7_4_9"/>
    <dbReference type="OMA" id="HYADGQN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="PTHR11575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="SSF55816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="3">
        <location>
            <begin position="227"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="5_nucleotid_C" evidence="4">
        <location>
            <begin position="521"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="725" mass="77489" checksum="F8702B514BBD92E8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR5</accession>
    <name>Q67QR5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycolate oxidase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH993</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH993"/>
    <dbReference type="EnsemblBacteria" id="BAD39978">
        <property type="protein sequence ID" value="BAD39978"/>
        <property type="gene ID" value="STH993"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH993"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_9_2_9"/>
    <dbReference type="OMA" id="CNDNMLA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="3">
        <location>
            <begin position="56"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="489" mass="52729" checksum="FF5ADDC5183441FA" modified="2004-10-11" version="1">MKGSDHQTTRRTSRKEGESMLTPEILAALRAAVGEEWCLTSKADLMAYSYDATPLYQRLPDAVVLPRTTEEVAAVLQIAHAHRIPVFTRGAGTNLAAGTVPAGGGIVLSLNRMNRILEIDQENLTATAEAGVITAELHKAVEAVGLFYPPDPGSMTSSTLGGNVMESAGGLRGLKYGVTKDYVMGLTAVTADGRIFRAGGKNVKDVAGYDLVKLICGSEGTLCVVTEVILKLLPMPEAKRTALAVFHDMEAAARTVSRIIAARIIPCTLEFMDNGTIRAVEDYVHLGLPTDAAALLLIQQDGPASVCDADIQRIAQICREEGATRVEHASDPAEQDRLMQARRFALSALARRRPTTILEDATVPRSQIAPMVAEIQRIAQKYDLDICTFGHAGDGNLHPTCMTDERNKEEIQRVEQAFAEIFAAALELGGTITGEHGVGEAKSPYLEWKVGPVGIELMKNIKKAFDPHGILNPGKLFAGETRKRVVVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS0</accession>
    <name>Q67QS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH988"/>
    <dbReference type="EnsemblBacteria" id="BAD39973">
        <property type="protein sequence ID" value="BAD39973"/>
        <property type="gene ID" value="STH988"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH988"/>
    <dbReference type="HOGENOM" id="CLU_2467873_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9438" checksum="F3E03100C73774B1" modified="2004-10-11" version="1">MVVAYFERLTPTRWPADAHIQTPCDPLVAAAEFSYSNPGFAGLLEVSVQGLVGGRPVLKPVLVQVDGPGRCRLAPPDSRLAVPTRELV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU3</accession>
    <name>FENR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferredoxin--NADP reductase</fullName>
            <shortName evidence="1">FNR</shortName>
            <shortName evidence="1">Fd-NADP(+) reductase</shortName>
            <ecNumber evidence="1">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH965</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QU3"/>
    <dbReference type="SMR" id="Q67QU3"/>
    <dbReference type="STRING" id="292459.STH965"/>
    <dbReference type="EnsemblBacteria" id="BAD39950">
        <property type="protein sequence ID" value="BAD39950"/>
        <property type="gene ID" value="STH965"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH965"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_9"/>
    <dbReference type="OMA" id="PEKVIYD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364979" description="Ferredoxin--NADP reductase">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <sequence length="336" mass="36683" checksum="7AF1805EB6E584FD" modified="2004-10-11" version="1">MSGTKELYDVTLIGAGPTGLFGVFYAGMRGMKTKVIEALPEVGGQLAALYPEKDIFDVAGFPRISAKRLVEQCKEQADSANPDATYVFNQRVDKLNRLEDGTFELVTHTGERHYSKAVIITAGIGAFEPNRIPNESARQYEGKGVFYSVTNLPQFEGKRVLVIGGGDSAVDYALMVEPIAAEVTLIHRRDGFRAHEESLKKLAASRVHVKVFYELRRVEGDGNWVKKATIFDNRTGEETTIDVDCVIIGTGFKASLGSMLEWGLEIENKRQIVVNSKGETNIPGVYAAGDICWYPGKIRLIATGFGEVATAVNNAKAFIDPGSAAFPGHSSEQRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU4</accession>
    <name>Q67QU4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRUD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH964</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195096.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH964"/>
    <dbReference type="EnsemblBacteria" id="BAD39949">
        <property type="protein sequence ID" value="BAD39949"/>
        <property type="gene ID" value="STH964"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH964"/>
    <dbReference type="eggNOG" id="COG0585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_9"/>
    <dbReference type="OMA" id="GFPNYFD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRUD" evidence="3">
        <location>
            <begin position="136"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="384" mass="43614" checksum="2F55102D985958A8" modified="2004-10-11" version="1">MRLKTTPEDFVVREAVDLHIRQKPARYRVYLLEKKGWNTADAVAAIARARRLPPGRIQYGGKKDRHAHTFQYVTIDHPADHSLSTPYYRLKAVGYATEPMAPGRVLANHFEVTVRELRPEEAARLVDGVRRVAAAGFPNYFDDQRFGSFDPRRGFIAEQMAQGRWEQALQIALTHVYPGEKSRAKARKRRLLEAWGDWATCLDLAETAFERRCFALLAEQPGGFRAALATARREEQEMWLSAWQAFLWNEVLRRLVAAAAGGDEPPAEAAGVAGPYRFARSTPELQQMVIPLPGRGMRFSDPRAGLILEDVLRERRLRPAQLEAELLPGLWLRSSPREAWVMPRSVRVDPPQPDERYPGFERVSLRFTLPRGAYATMLIKAAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY9</accession>
    <name>Q67QY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitrate reductase component, cytochrome C-type protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195051.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH919"/>
    <dbReference type="EnsemblBacteria" id="BAD39904">
        <property type="protein sequence ID" value="BAD39904"/>
        <property type="gene ID" value="STH919"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH919"/>
    <dbReference type="eggNOG" id="COG3005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096753_0_1_9"/>
    <dbReference type="OMA" id="EFRTTHA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3820.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005126">
        <property type="entry name" value="NapC/NirT_cyt_c_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038266">
        <property type="entry name" value="NapC/NirT_cytc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03264">
        <property type="entry name" value="Cytochrom_NNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="SSF48695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269943" evidence="3">
        <location>
            <begin position="34"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_NNT" evidence="4">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="141" mass="15329" checksum="A63D633683E68701" modified="2004-10-11" version="1">MTKRGWILVAVAAAALVLLIGGPSVITATGAEPATCATCHSMQEFRTTHAQSDHAAVACTQCHLPQGLASIPAKYEAGFKHVWATITGYEDIQLSPESEQILLDNCIACHVQTDHVRVPENRGCLNCHFDDPHGVREDRKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R17</accession>
    <name>Q67R17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallophos_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195023.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH891"/>
    <dbReference type="EnsemblBacteria" id="BAD39876">
        <property type="protein sequence ID" value="BAD39876"/>
        <property type="gene ID" value="STH891"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH891"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074761_0_1_9"/>
    <dbReference type="OMA" id="YLCHATP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="26657" checksum="8ABB0CEF69947633" modified="2004-10-11" version="1">MRIAIISDVHGNLTALEAVLADIDPETVDALICLGDLAFKGPQPAECIRRIRELGIPCLHGNTDVALVMAAGRKPRRPLPAGFSLPDPLPPSLAWPLAQLSDADVEYLAGLPFSHRLEADGQTVLFVHASPQDAMTGIAPTMGPDEIRPLLREEQADWIIAGHVHQAYAFRFEGRWLANPGAVGFSLDGDGRAAYAVLDTARSRFELRRVAYDVDAAVAAARQRSFYADPDRYGEALRAGTWPADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R30</accession>
    <name>Q67R30_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195010.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH878"/>
    <dbReference type="EnsemblBacteria" id="BAD39863">
        <property type="protein sequence ID" value="BAD39863"/>
        <property type="gene ID" value="STH878"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH878"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_2_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="130" mass="15030" checksum="B7B56ABC1E72B12D" modified="2004-10-11" version="1">MEQVVIFRDIDMMGHVNNAVYASYFEAARVEYFYQLLDGKTDRFPFIVAELTITYKSPAYLRERLLIGQRVSEIRNSSMVIDGQIVEKTTGRLVATSRVVGVHYDYKEQRPVPIPPELRDLIARFEGRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R40</accession>
    <name>Q67R40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Efflux transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH868</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH868"/>
    <dbReference type="EnsemblBacteria" id="BAD39853">
        <property type="protein sequence ID" value="BAD39853"/>
        <property type="gene ID" value="STH868"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH868"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_16_0_9"/>
    <dbReference type="OMA" id="GVWIDRY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="214"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="43020" checksum="D54A051A7EB58367" modified="2004-10-11" version="1">MDTGWKRNVVLFLGSQTLSLLGTSLVQYAIFWYITLTTQSGAAMTVYIICGFLPTFFLSPFAGVWADRFSRRRLIAASDAAIAAATLVLALLFMAGYDSLWLLYVMAAVRAAGSGVQGPAVSALIPDIVPQEQLTRVNGANSSIQAIVTLVSPMLSGALFSQAPLAAIFLIDVATAAAAIAILLALVRTPVHAKAREAQATSYFADLAEGFRYIAGQAYIKAFFAFCALFFVLSAPVSFLTPLQVARTFGDDIWYLTAIEVGFSVGMILGGALTAAWGGLRNRVHTMALATLLFGACTFALGVVRDFRVYLAIMGLSGITMPLFNTPATVLLQENVDENMLGRVFGVMNMLASAMMPLGMLVFGPLADLIRIEAMLVATGILMALQGVLLLSNRVLVRAGVRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC5</accession>
    <name>Q67RC5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spore coat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH783"/>
    <dbReference type="EnsemblBacteria" id="BAD39768">
        <property type="protein sequence ID" value="BAD39768"/>
        <property type="gene ID" value="STH783"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH783"/>
    <dbReference type="eggNOG" id="COG5337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066387_0_0_9"/>
    <dbReference type="OMA" id="HLNAEYK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014867">
        <property type="entry name" value="Spore_coat_CotH_CotH2/3/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40050">
        <property type="entry name" value="PTHR40050"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08757">
        <property type="entry name" value="CotH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0946">Virion</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="36823" checksum="59CAA7B8860124B7" modified="2004-10-11" version="1">MRIRGAHSRKFPKRSLQVTLRGARLPDEPPAGHTVQRIHLNADFVDPTLMRSALSYTLFPLLGVDAPRWRHVLPTVSGEPAGLYVGLESVDRDFCLRRGWPPGPIFYAINRNANFGLISPFTRTLKEPLDRGYHPVEGADTEPIRQMLLDLNLADDASFLQAAARWFDLERYVHWVIGAVFVGNRDGFVHNYALYLEPGERRFRIIPWDYDATWGIDIHGRPARLDRVPVTGWNKLSYRLFAHPEGRRRFRRAFLRALDGPLAPDSVLPLIDRLASQVEPWRERDPYLRRADFPREVERLRRWAVERRAVLLRELASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF1</accession>
    <name>Q67RF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Na+-driven multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH757"/>
    <dbReference type="EnsemblBacteria" id="BAD39742">
        <property type="protein sequence ID" value="BAD39742"/>
        <property type="gene ID" value="STH757"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH757"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_3_9"/>
    <dbReference type="OMA" id="GEICEIA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="416" mass="43629" checksum="A4100DA616D2784F" modified="2004-10-11" version="1">MVDMAFVGRVGAYAVAAVGVATQPLWLTFGLAGALGVGIIAVVSRLAGARDDEGVERAASTASWLGLGLALLLGAGLYFGAPWIVKVMGAPPDVYPHGVIYLQVLVPGLIGHYWFSAMSAALRAVGETRIPMLLALVANAVNVVLDWLLIFGNLGFPALGLAGAALASSVARFTEAAGLLVVLLVRSGPVALRWRTLWRFSPDLSARIVRVAVPSAVERSASTLSLVVFAILINRLGTVAIATQQIAYVVEDLIWLVAFGLGTSCATLIGQSLGAQDPERARLAMLEGLRVGAAFTLTVAASFLLMPGVYMRIFTTDAQVIALGTMALRVAAAADIPMGLVLILSGGLQGAGDTRAAAVITLVGSWGVRLTLAYLMIDVLGWGLYGAWTAAACDWALRLALFWNRFARGRWKEMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG9</accession>
    <name>Q67RG9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH739</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH739"/>
    <dbReference type="EnsemblBacteria" id="BAD39724">
        <property type="protein sequence ID" value="BAD39724"/>
        <property type="gene ID" value="STH739"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH739"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_14_9"/>
    <dbReference type="OMA" id="ANATEHE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="382" mass="38942" checksum="4BA2E975F073C78C" modified="2004-10-11" version="1">MLVFTSFYDQLAWSPIVAWLAMSFGAPEATFWAATAYSAANLLGNVLFGVLADRVDRMAVAGAGLLAMIGTGLAHLLPDSTAGLVGVRALHGLAIAGVAPAAMASVTDGLPRNQRGAAMARMGLVIAIASMLASAASGRLLTSLGAAASVYMLAALVGTVGIFTVVIRRWLEPPPVRKGAEGRGEPAPGRVNMPGMAAAGLVAYALMFLQNVLFSAYPLKGYEIGLTSATVGMTLSAFGLGAVIAFVPPLSRASDRWGRHQPIFVGMALAGAGHLGLAQGTTPLTFAAWLFIYGLGFGLVFPAVSALNADAAGAGRRGVAYGLLSAAFSLGSISGPPVTQALAELASPFSVAGWLGLATSGFVSLWYYLAVRPAEEAARQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ4</accession>
    <name>Q67RQ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH654"/>
    <dbReference type="EnsemblBacteria" id="BAD39639">
        <property type="protein sequence ID" value="BAD39639"/>
        <property type="gene ID" value="STH654"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH654"/>
    <dbReference type="eggNOG" id="COG2014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076326_1_0_9"/>
    <dbReference type="OMA" id="GICFTPI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="DUF364" evidence="1">
        <location>
            <begin position="115"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="27338" checksum="E20D2215F845E18A" modified="2004-10-11" version="1">MLEAAAPALRGHRIRAARAGARIVGVVLDDGRFGAGYHLPGERARGLLDDAAPAGLRTCADPGAGLEAVPGQPALEVARRCLLGESAFRRALGVAICNAAADPDPARCLVDPDAAAAVPARPTDTVGFVGYVGSVVRRVQAEAARTIIFDRSQEDGEDVYPESLQPELLPRCDLVFITGSALINRTLERLLTYCTRAREVVLIGPSTPLYPEAFLGSGVTVLAGFRWQVADPDAALEQVAAGAHFKHLKGRTSVTLRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR7</accession>
    <name>Q67RR7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH641</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194774.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH641"/>
    <dbReference type="EnsemblBacteria" id="BAD39626">
        <property type="protein sequence ID" value="BAD39626"/>
        <property type="gene ID" value="STH641"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH641"/>
    <dbReference type="eggNOG" id="COG1104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_0_9"/>
    <dbReference type="OMA" id="IIYGQSE"/>
    <dbReference type="OrthoDB" id="839689at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="9">
        <location>
            <begin position="5"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="39836" checksum="68B9B54C760269E8" modified="2004-10-11" version="1">MERPIYLDYNATTPVDPAVVEAMLPYLTDHYGNPHSTHRLGAVAAAAVATAREQVAGLIGASAGEIVFTSCASESINLAVKGVAFARGRGHIITSAIEHPATLEACRYLERHFGFALTVLPADEQGRIDPDAVRRALRPDTILITLLHAQNETGVIQPVEEVGRIAREAGVLFHVDAAQSCGKIPVDVRAIGCDLLTVAGHKLYAPKGVGALYVRSGVVLHPLIHGADYEGGRRAGTENVPYLVALGRAAEIAEAKLAIGEGRRLAELRDRLQQRLAAALPIRVTGLGAPRLPNTLHLLVEGLVGNDLLDAAPEVAASTGSACHAGVSEPSAVLLAMGLAPEEALGSVRLSLGRYTTAEEVDAAADALIAAARRLRPAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY3</accession>
    <name>Q67RY3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="9">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glycyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="9">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">glySB</name>
        <name evidence="9" type="synonym">glyS</name>
        <name evidence="11" type="ordered locus">STH575</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194709.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH575"/>
    <dbReference type="EnsemblBacteria" id="BAD39560">
        <property type="protein sequence ID" value="BAD39560"/>
        <property type="gene ID" value="STH575"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH575"/>
    <dbReference type="eggNOG" id="COG0751">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007220_2_2_9"/>
    <dbReference type="OMA" id="LPIPKRM"/>
    <dbReference type="OrthoDB" id="213210at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00255">
        <property type="entry name" value="Gly_tRNA_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="PTHR30075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01045">
        <property type="entry name" value="TRNASYNTHGB"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR_1" evidence="10">
        <location>
            <begin position="590"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="700" mass="77795" checksum="9F257F3866C8AACF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ8</accession>
    <name>Q67RZ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH560"/>
    <dbReference type="EnsemblBacteria" id="BAD39545">
        <property type="protein sequence ID" value="BAD39545"/>
        <property type="gene ID" value="STH560"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH560"/>
    <dbReference type="eggNOG" id="ENOG5032N2F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1365621_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021238">
        <property type="entry name" value="DUF2620"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10941">
        <property type="entry name" value="DUF2620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="21485" checksum="FEEB497ADFC1B57F" modified="2004-10-11" version="1">MGLSYPILPLRPDPFSSVRPAANEAGRDVSPFGHPGTRLWYRPTLPAHNGADAEDRLTLFRGGLYNIQETVDWKGWIGMIRFAVGGQVDKKKVADLVRKVGGSAVQVTEHSDLEAAMQVKNGKADYYIGACHTGGGGLAMAIAMLGKARCLTVSMPGRPPKEEEIRKAVADGVKAFAFTADHAEQAVSMIVRAILERGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S04</accession>
    <name>Q67S04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phosphotriesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Phosphotriesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH554"/>
    <dbReference type="EnsemblBacteria" id="BAD39539">
        <property type="protein sequence ID" value="BAD39539"/>
        <property type="gene ID" value="STH554"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH554"/>
    <dbReference type="eggNOG" id="COG1735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054760_1_1_9"/>
    <dbReference type="OMA" id="MVKCGFI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009056">
        <property type="term" value="P:catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00530">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017947">
        <property type="entry name" value="AryldialkylPase_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001559">
        <property type="entry name" value="Phosphotriesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10819">
        <property type="entry name" value="PTHR10819"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02126">
        <property type="entry name" value="PTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016839">
        <property type="entry name" value="PhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01322">
        <property type="entry name" value="PHOSPHOTRIESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51347">
        <property type="entry name" value="PHOSPHOTRIESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601559-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="304" mass="33329" checksum="2B1653C7188A4EE7" modified="2004-10-11" version="1">MAHIMTVLGPLDPKELGPTLMHEHLFIDLSGRKKDPDTVLDDLDLACEEVAHLQRAGGRALVEVTCHGMGRRPELLREVARRTGLQVVAATGFYQQAYHPPYVAERSAEELAELLMRDIQEGMDGTDVKPGILAEIGTSKGEIRPDEAKVFRAVALVHKWTGLPISTHCTLGTMGLEQLDLLESLGVDPARVVIGHQDLTDDLETHVALARRGATVAYDTAGKESYMPDAVRVRLITGMLERGLADRVVLSMDVTRRSHLKANGGYGYSYLFDRLVPALRAAGVSEAELEQMLVLNPRRILTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S66</accession>
    <name>Q67S66_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH492</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH492"/>
    <dbReference type="EnsemblBacteria" id="BAD39477">
        <property type="protein sequence ID" value="BAD39477"/>
        <property type="gene ID" value="STH492"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH492"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_9"/>
    <dbReference type="OMA" id="HQGNANF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="129"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="21934" checksum="2EE58BD94D7949A1" modified="2004-10-11" version="1">MVRQGLATYFELQDDFEVVGEAADGRQAVEMTRTLAPDVVLMDLMMPGLDGLSALRELKGTGAKVIMLTSFLDLEKAMACIEEGALGFLTKDIEPADLVDAIRAVHRGEPRLHPEVMKRLMVRTAQPHQPSKRDLLTPRELDVLRALAHGLTNREIAERLVISETTVKTHISSILSKLQLTDRTQAALWAVREGLVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF0</accession>
    <name>Q67SF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative chromate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH408"/>
    <dbReference type="EnsemblBacteria" id="BAD39393">
        <property type="protein sequence ID" value="BAD39393"/>
        <property type="gene ID" value="STH408"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH408"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_4_0_9"/>
    <dbReference type="OMA" id="TPEYNNG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="182" mass="19911" checksum="365FF04C7D28A3A9" modified="2004-10-11" version="1">MYVRVAILVGSLRADSFNMQLARTICERYRDRMEAEILDIGTLPHFDQDAEQDPPPAVADFKRRIREADGVIIVTPEYNWSVPGVLKNALDWASRVDKVFIGKPVMIAGATPGMLGTVRAQLHLREILSSPGLQARVLPPGGNEVLVSLAPQKFAGGRLVDEATLSFLDGVVDKFIAFIASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH7</accession>
    <name>Q67SH7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Signal peptidase I</fullName>
            <ecNumber evidence="4 6">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH381</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH381"/>
    <dbReference type="EnsemblBacteria" id="BAD39366">
        <property type="protein sequence ID" value="BAD39366"/>
        <property type="gene ID" value="STH381"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH381"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_5_1_9"/>
    <dbReference type="OMA" id="ACDAYIK"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="8">
        <location>
            <begin position="36"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="22382" checksum="E1CE9975D3BE6957" modified="2004-10-11" version="1">MQGGGRKARSRGGTEQGRIRSGGNSMAEKLRPLLTLLKDVLYGVLLWLLIITFVGQVREVPTGSMEPTILVGDRFWTDKLILRFTSIRRGDIVVFDPPPQVQAQYPYIKRVIGLPGETVEVRDGLVFINGEPLDEPYIAEPPRYTYGPVTIPEGQYFVLGDNRNLSNDSHEWGLLNRERIFARAVYRIWPLSRIGSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN3</accession>
    <name>Q67SN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH325"/>
    <dbReference type="EnsemblBacteria" id="BAD39310">
        <property type="protein sequence ID" value="BAD39310"/>
        <property type="gene ID" value="STH325"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH325"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397347_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="693" mass="76377" checksum="3253F949F82DB837" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ6</accession>
    <name>Q67SQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH302"/>
    <dbReference type="MEROPS" id="S12.950"/>
    <dbReference type="EnsemblBacteria" id="BAD39287">
        <property type="protein sequence ID" value="BAD39287"/>
        <property type="gene ID" value="STH302"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH302"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_1_1_9"/>
    <dbReference type="OMA" id="FTTEYNH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="42"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="41800" checksum="74F877A027EC7B5A" modified="2004-10-11" version="1">MNAVPIVDALHETRWPRGLRAAGPAEAGFDPQRLEQAIALLEAEIAAERLPGAVVVIARDGKVAVHRALGWAAVYPERRPMRPETLFDLASLTKVIATLPAVLRLIERGDIRLDDPLHLFFPEYQGEGREEIRIRHLLTHTAGLQPFSQALRDPSRPREELIARIATLPLEAPPGTRIIYSDLGFILLGELVARVSGQPLERFVQEQVHGPLGFTETGFLPTGERAARAAATEYRERLGRYQCGEVHDETATALGGIAGHAGLFSTAPEVAAYGQMWLDGGAGVLSPAMVAAATRDQTPHIQGPEHRGLGWIVAREDSESLSCGDLFSPGSFGHTGFTGTSLWVDPHRRLVVALLTNRVHFGRTPHIIRLRPRFHNAVAAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR4</accession>
    <name>Q67SR4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194429.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH294"/>
    <dbReference type="EnsemblBacteria" id="BAD39279">
        <property type="protein sequence ID" value="BAD39279"/>
        <property type="gene ID" value="STH294"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH294"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_862891_0_0_9"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="189"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="35899" checksum="059756C5181ABAFD" modified="2004-10-11" version="1">MIRTMEAADLPAVHTLWNRTTLSREVLYKPLACERFQALFVSAGDHEVKCNVVAEQDGQILGFGSAYHNTGTDRGYITFILVDPAHRRRGVGTALLRALEHRLRAFPTIVRIDLNFFNPINLEWFIPGTPGHDHPNAPGVDVSSPAFPFFTARGYAETSRQYSYYRLLADFQFQPKIAERAAALAAQGISITYYDPARHYGLSELFDDLGNEDWRDKVMTNVNGPRPYPLLIVEQGGRVGGFAGPLYVQESGRGYFAGIGVHSELRGVGAGSVLFSALCQGLRDIGATFMSLFTGRENVARKIYESAGFQVVHEWACMRKAMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV6</accession>
    <name>Q67SV6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative biotin synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH252"/>
    <dbReference type="EnsemblBacteria" id="BAD39237">
        <property type="protein sequence ID" value="BAD39237"/>
        <property type="gene ID" value="STH252"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH252"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1011662_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034422">
        <property type="entry name" value="HydE/PylB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43726">
        <property type="entry name" value="PTHR43726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="29757" checksum="5BC32DA06D120F29" modified="2004-10-11" version="1">MIVVIGPILRRAAAGRTPSRREILLMLRAEDEEYRSLLEVAGRICRQKKGEEPSLRFLVDLEVSDPQRIREDVVHAWRTGCASVLLCASSGYDAVDEVAALIRDIAEETDMQVILGLGERSYEVYALWRHAGAVEYLLPHDSCNPDLFSQLHPGHSPAVRLTRGLWLKGLGYRVTGGICVGIDGETDEDLADDLEVLRNAGLTGVMVRSAGGAGDLLRVLAITRLCLPDADLWVATDDPVLQARALGCGANILVTAHPAGSDHEVPRALSHGISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T29</accession>
    <name>Q67T29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39164">
        <property type="protein sequence ID" value="BAD39164"/>
        <property type="gene ID" value="STH179"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH179"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_18_3_9"/>
    <dbReference type="OMA" id="IFRCEFA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="150" mass="16423" checksum="6A9856BA5009C3A7" modified="2004-10-11" version="1">MRLAAILRTVLAVGYDLFLKLNPRKVAAHAVICDDQGRVLALKSRYADVWLLPGGGLKPGEHLDEGLRRECLEELGAEVAVEALTGVYYVERSAAYVGVFRCRLDGQPIRLSHEHEVYDWVLPDQLPPAARAMAADALRPAGSPVVARLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T55</accession>
    <name>Q67T55_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">APH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH153"/>
    <dbReference type="EnsemblBacteria" id="BAD39138">
        <property type="protein sequence ID" value="BAD39138"/>
        <property type="gene ID" value="STH153"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH153"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2107748_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="12888" checksum="C15B13A2A82019E6" modified="2004-10-11" version="1">MLLHGDFGWRNLLLCDGGTLVLLDFERAVIGPAWLDLAKCLDRELRLPQDREGFLQGYEKASGMPLARPPEAYLTCLRLWVAAGILLFTSKHADEPFAEHGRRLLQQVTGDLDLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD7</accession>
    <name>RL31_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpmE</name>
        <name type="ordered locus">STH71</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL31 family. Type A subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD39056" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39056.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_043712917.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TD7"/>
    <dbReference type="STRING" id="292459.STH71"/>
    <dbReference type="EnsemblBacteria" id="BAD39056">
        <property type="protein sequence ID" value="BAD39056"/>
        <property type="gene ID" value="STH71"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH71"/>
    <dbReference type="eggNOG" id="COG0254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114306_4_3_9"/>
    <dbReference type="OMA" id="WYPDAKV"/>
    <dbReference type="OrthoDB" id="2014569at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000259233" description="50S ribosomal protein L31">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="67" mass="7466" checksum="88F9EFA8CC38B8AB" modified="2006-10-31" version="2">MKAGIHPKTQKTTITCGCGATYEVLSTKENIRVEICANCHPFYTGSRGATRVEAGSRIDRFRKKYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ6</accession>
    <name>Q67TJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type dipeptide/oligopeptide/nickel transport systems periplasmic components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH12</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH12"/>
    <dbReference type="EnsemblBacteria" id="BAD38997">
        <property type="protein sequence ID" value="BAD38997"/>
        <property type="gene ID" value="STH12"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH12"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_0_4_9"/>
    <dbReference type="OMA" id="YDFFTWE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269389" evidence="1">
        <location>
            <begin position="28"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="94"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD38997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="569" mass="60101" checksum="1B2DAAAF12276848" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q3V820</accession>
    <name>Q3V820_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyridoxal phosphate biosynthetic protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PdxA family. PdxA2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2235"/>
    <dbReference type="EnsemblBacteria" id="BAD41220">
        <property type="protein sequence ID" value="BAD41220"/>
        <property type="gene ID" value="STH2235"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2235"/>
    <dbReference type="eggNOG" id="COG1995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_215488_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="PTHR30004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="50" mass="5042" checksum="BC14E59C481FB334" modified="2005-10-11" version="1">MNVTAGLPIIRTSVDHGTAFDIAGKGIADEGSMIAAIELAGRLSGVGQQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J68</accession>
    <name>Q67J68_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative serine/threonine protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3301"/>
    <dbReference type="EnsemblBacteria" id="BAD42282">
        <property type="protein sequence ID" value="BAD42282"/>
        <property type="gene ID" value="STH3301"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3301"/>
    <dbReference type="eggNOG" id="COG0639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023125_4_0_9"/>
    <dbReference type="OMA" id="IGHTITF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="2"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="22968" checksum="B50B5AF091B445A0" modified="2004-10-11" version="1">MVSDIHGESRRLLKVLEMAHYNPSTDRLFLLGDYIDRGSDSKGTLEIVERLVRSGAVALLGNHDLMLVSTYYDPSNLQRWVYNGGYTTLAEFGGFIPERWVRFLEGLPTHHEEPDCIMVHAGLAPGVPLSEQSDQDKVWIREPFLSQYRGKRVYFGHTPTCLLHGEERWEPWYGEDKVGIDTGAVYGGVLTLLDIDTGQVWTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J76</accession>
    <name>Q67J76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3293</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197405.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3293"/>
    <dbReference type="EnsemblBacteria" id="BAD42274">
        <property type="protein sequence ID" value="BAD42274"/>
        <property type="gene ID" value="STH3293"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3293"/>
    <dbReference type="eggNOG" id="COG1145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018240_2_0_9"/>
    <dbReference type="OMA" id="CMRCIQV"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036991">
        <property type="entry name" value="Fe_hydrogenase_ssu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013352">
        <property type="entry name" value="Fe_hydrogenase_subset"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="SSF53920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02512">
        <property type="entry name" value="FeFe_hydrog_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="456" mass="50062" checksum="3C861BEE775DE4A3" modified="2004-10-11" version="1">MIFIDQELCTGCRRCAEVCPVDAIVGEPGKPQSVDQEICVMCGQCVQVCSSYGSIWDDGLTPREQKLAERGLLPSVKEPLFAAWNEGRAKLVKEALADPDRYVMVQVAPAVRVAIAEEFGMPLGSLAPGKMAAALRRLGFDRVYSTDFTADLTIMEEGNELIERVTKGGTLPMFTSCCPAWVKYLEQTYPELLPHLSTCKSPQQMAGALFKTYGAKVDNVDPAKVYSVSVMPCTCKDFESGRPEMKASGYRDVDVVITTRELAHLIKDAGIDFNALPEEEYDRPLGLYTGAGTIFGATGGVMEAALRTAYEVIAKKPIPELDLKFVRGGEGVRYATVKIEDLELKVCVVSGLKNVAPVLEQVKAGKADFHFMEVMTCPVGCVSGGGQPKALLPSDRLCARMARTESTYRHDAQLPHRKSNENPDIKKIYKEFLGKPLGEKSHHLLHTSYTPRRRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC4</accession>
    <name>Q67JC4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pur operon repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3244</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3244"/>
    <dbReference type="EnsemblBacteria" id="BAD42226">
        <property type="protein sequence ID" value="BAD42226"/>
        <property type="gene ID" value="STH3244"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3244"/>
    <dbReference type="eggNOG" id="COG0503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088227_0_0_9"/>
    <dbReference type="OMA" id="YMLERPR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045982">
        <property type="term" value="P:negative regulation of purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015265">
        <property type="entry name" value="PuR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010078">
        <property type="entry name" value="PurR_Bsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF2">
        <property type="entry name" value="PTHR43864:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09182">
        <property type="entry name" value="PuR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01743">
        <property type="entry name" value="purR_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="PuR_N" evidence="6">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="123"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="29343" checksum="2AA52C77C6E2665B" modified="2004-10-11" version="1">MNRSQRLVAIMKLLSERPGELLPLSFFTERFGAAKSTISEDLALVKEALEADGSGRLRTHAGAAGGVQYWPLPSREEEQETLLELCRLLSDPGRILPGGFVYMTDLITHPIWSARIGAIMAARFIDAEPDVVLTVETKGIPLALMVARALGLPMVVARREGRVTEGPSVTLHYISGSRRIHTMTVGLRALWRGARVLVVDDFMKAGATARAMVDVAGEMGASVAGVGVFVSTAEPARKQVQRYVSLLTLEQVDEVARTVRVVPADRLTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC9</accession>
    <name>Q67JC9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="6">RPPK</shortName>
            <ecNumber evidence="6">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="6">P-Rib-PP synthase</shortName>
            <shortName evidence="6">PRPP synthase</shortName>
            <shortName evidence="6">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prs</name>
        <name evidence="8" type="ordered locus">STH3239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3239"/>
    <dbReference type="EnsemblBacteria" id="BAD42221">
        <property type="protein sequence ID" value="BAD42221"/>
        <property type="gene ID" value="STH3239"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3239"/>
    <dbReference type="eggNOG" id="COG0462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_4_0_9"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="7">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribose-5-phosphate binding" evidence="6">
        <location>
            <begin position="227"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="34820" checksum="BA885278F9C5344B" modified="2004-10-11" version="1">MVPYQDRKLKIFSGNANLPLARAIADHMGISLGNMNVGRFSNGEIRVDINESVRGTDCFVIQSGVHPVNENVMELLVIGDALRRASARRITAVVPHFPYARQDRKARGREPITAKLLANLITTAGFRRLLTIDLHAAQIQGFFDTPVDHLHAGPLIANYFRQKGLQNAVVVSPDTGGVPRARDLAERLGVGLAIIDKRRPEPNVAEVMNIIGDVEGKTAILVDDLIDTAGTIVKGAEALLERGATEVYAACTHPVFSGPAYERLAASPIKEIVVTDTIPVDADKMGGKLRVVSVAPLLGKAIIRIHEDLSVSKIFEEEPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD8</accession>
    <name>Q67JD8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Non-specific DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3230</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197343.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3230"/>
    <dbReference type="EnsemblBacteria" id="BAD42212">
        <property type="protein sequence ID" value="BAD42212"/>
        <property type="gene ID" value="STH3230"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3230"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_1_9"/>
    <dbReference type="OMA" id="PAHEGIN"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="89" mass="9518" checksum="B1DB94478993725A" modified="2004-10-11" version="1">MNKQDLVASVAEKSGLTKKDAEKALNAVVESIKEALKKGDKVSLVGFGTFEVRNRAARSGRNPQTGEPIKIPAGKVPAFRPGKELKESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ9</accession>
    <name>Q67JJ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3169"/>
    <dbReference type="EnsemblBacteria" id="BAD42151">
        <property type="protein sequence ID" value="BAD42151"/>
        <property type="gene ID" value="STH3169"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3169"/>
    <dbReference type="eggNOG" id="COG2947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041799_2_1_9"/>
    <dbReference type="OMA" id="LWDGIRN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="15413" checksum="E8D85ADE95485F3C" modified="2004-10-11" version="1">MKTEPTVYSYDDLERAGRDLWDGVRNAAAARNMRAMRPGDLFLFYHTGDERAVVGVGRIVSEPYPDPTDSEGRWVAVDVEPLYRFRTPVTLADVKADPRFADWALVRQSRLSVMPVSQEQWAWLHEMGGTELREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL3</accession>
    <name>Q67JL3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupredoxin_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3155"/>
    <dbReference type="EnsemblBacteria" id="BAD42137">
        <property type="protein sequence ID" value="BAD42137"/>
        <property type="gene ID" value="STH3155"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3155"/>
    <dbReference type="eggNOG" id="COG4454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141684_0_0_9"/>
    <dbReference type="OMA" id="GCETSAM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028096">
        <property type="entry name" value="EfeO_Cupredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13473">
        <property type="entry name" value="Cupredoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupredoxin_1" evidence="1">
        <location>
            <begin position="46"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14022" checksum="3DBD0FE23D2E136F" modified="2004-10-11" version="1">MILGRKGCETSAMRKLASLLVVALLSLGVLTGCGGGGGGNVKELTVEMGINGEMKFTPDVLEATKGETIKITLVNKDPNVAHNFLIPAFNVNSGQVPPGQTATVEVKVTQSGEHDIICDVPGHREAGMVGKLIVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-29" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX4</accession>
    <name>ECFA2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Energy-coupling factor transporter ATP-binding protein EcfA2</fullName>
            <shortName evidence="1">ECF transporter A component EcfA2</shortName>
            <ecNumber evidence="1">3.6.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ecfA2</name>
        <name type="synonym">cbiO2</name>
        <name type="ordered locus">STH3044</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197159.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JX4"/>
    <dbReference type="SMR" id="Q67JX4"/>
    <dbReference type="STRING" id="292459.STH3044"/>
    <dbReference type="EnsemblBacteria" id="BAD42026">
        <property type="protein sequence ID" value="BAD42026"/>
        <property type="gene ID" value="STH3044"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3044"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="PKYLFCD"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030946">
        <property type="entry name" value="EcfA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04521">
        <property type="entry name" value="ECF_ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51246">
        <property type="entry name" value="CBIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000288015" description="Energy-coupling factor transporter ATP-binding protein EcfA2">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="3"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01710"/>
        </source>
    </evidence>
    <sequence length="288" mass="31388" checksum="778A5DDACAEF7167" modified="2004-10-11" version="1">MPIVFEAVSHIYQPGTPFAWKALDDVSLTIPDGEFWGIIGPTGSGKSTLIQHMNGLLRPTTGRVLVDGLDLSDRKTDLRKVRQRVGLVFQYPEHQLFGETIFEDVAFGPRNMQLGAAEVERRVMTALERVGLDPAMKDRSPFGLSGGQARRVALAGVLAMEPRVLILDEPTAGLDPQGRREILDLVGGFARTGMTVVLVSHSMDDVAEYADRVLVMHRGRVHMLGTPWELFARRAELEAIGLGVPAAVALADKLRERGWSIPPDVVTMDEAVAAIRGVLAGRKGADRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K78</accession>
    <name>Q67K78_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="16">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="16">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="16">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">nnrE</name>
        <name evidence="14" type="synonym">nnrD</name>
        <name evidence="18" type="ordered locus">STH2937</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15 16">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14 16">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 14 16">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="15 16">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="16"/>
    <dbReference type="EC" id="5.1.99.6" evidence="16"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197053.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2937"/>
    <dbReference type="EnsemblBacteria" id="BAD41920">
        <property type="protein sequence ID" value="BAD41920"/>
        <property type="gene ID" value="STH2937"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2937"/>
    <dbReference type="eggNOG" id="COG0062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_9"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="OrthoDB" id="1748633at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 14 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 14 16" id="KW-0521">NADP</keyword>
    <keyword evidence="7 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 16" id="KW-0630">Potassium</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="17">
        <location>
            <begin position="9"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="15">
        <location>
            <begin position="58"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="15">
        <location>
            <begin position="128"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="418"/>
            <end position="422"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAD41920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="520" mass="53343" checksum="19EE900852D07E5D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG5</accession>
    <name>Q67KG5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2848</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2848"/>
    <dbReference type="EnsemblBacteria" id="BAD41833">
        <property type="protein sequence ID" value="BAD41833"/>
        <property type="gene ID" value="STH2848"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2848"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_9"/>
    <dbReference type="OMA" id="VERQAFF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="132"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="298"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="4">
        <location>
            <begin position="441"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="712" mass="77583" checksum="5C213DDF44AC5680" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI8</accession>
    <name>DNLJ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ligA</name>
        <name type="ordered locus">STH2825</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196944.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KI8"/>
    <dbReference type="SMR" id="Q67KI8"/>
    <dbReference type="STRING" id="292459.STH2825"/>
    <dbReference type="PRIDE" id="Q67KI8"/>
    <dbReference type="EnsemblBacteria" id="BAD41810">
        <property type="protein sequence ID" value="BAD41810"/>
        <property type="gene ID" value="STH2825"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2825"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_9"/>
    <dbReference type="OMA" id="HDVEHEI"/>
    <dbReference type="OrthoDB" id="241401at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000313476" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="594"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="673" mass="74734" checksum="E1AEF232FA16FFB8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN6</accession>
    <name>NUOB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit B 2</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit B 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit B 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoB2</name>
        <name type="ordered locus">STH2777</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196896.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KN6"/>
    <dbReference type="SMR" id="Q67KN6"/>
    <dbReference type="STRING" id="292459.STH2777"/>
    <dbReference type="EnsemblBacteria" id="BAD41762">
        <property type="protein sequence ID" value="BAD41762"/>
        <property type="gene ID" value="STH2777"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2777"/>
    <dbReference type="eggNOG" id="COG0377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_9"/>
    <dbReference type="OMA" id="AGWVRKS"/>
    <dbReference type="OrthoDB" id="1904620at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000358487" description="NADH-quinone oxidoreductase subunit B 2">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01356"/>
        </source>
    </evidence>
    <sequence length="182" mass="19911" checksum="5822DFBC320726B8" modified="2004-10-11" version="1">MSQARHELDWLNDEVVVSEIDVVNENVIAGKLPDVIKKVLGWGYGNSTWPLGFGIACCAIEMMAACSSKYDIARFGSEAMRMSPRQADVMIVAGTVNEKMAVVVRRLYDQMSEPKFVIAMGACASNGGPYWDTYNVVDGVHKVVPVDVYVPGCPPRPEALLQGLMEIQEMIKTGKRGLITKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS8</accession>
    <name>Q67KS8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphoglucomutase/phosphomannomutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2734</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196853.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2734"/>
    <dbReference type="EnsemblBacteria" id="BAD41719">
        <property type="protein sequence ID" value="BAD41719"/>
        <property type="gene ID" value="STH2734"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2734"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_0_0_9"/>
    <dbReference type="OMA" id="PQDNGYK"/>
    <dbReference type="OrthoDB" id="637615at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="8">
        <location>
            <begin position="44"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="9">
        <location>
            <begin position="211"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="10">
        <location>
            <begin position="323"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="7">
        <location>
            <begin position="504"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="586" mass="63478" checksum="A1AA0F05F98F9E4F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT3</accession>
    <name>Q67KT3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2729</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2729"/>
    <dbReference type="EnsemblBacteria" id="BAD41714">
        <property type="protein sequence ID" value="BAD41714"/>
        <property type="gene ID" value="STH2729"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2729"/>
    <dbReference type="eggNOG" id="COG2972">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_12_9"/>
    <dbReference type="OMA" id="NWAVENE"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="73"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="197"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="368" mass="39035" checksum="8EDA78A078BEE9CD" modified="2004-10-11" version="1">MEGRMEERRTLGLQWQLVAVTAAAGLLASGLAVFGVWVALQQGFGWNEALVAGVAAGVAGGLAAAAWSFQLARGIKRRLWNAGDLAARIRRGDLSARLPVSDADEIGELEMQLNEMAAYLEQAVGQLSRLAEQNRLLAEEAGRGAALEERARIARDLHDTVNQQLFVLSLRAAAVRKRVGAASGAAAGGDGAGVEPQLVTELANLEELARTAHSQIRELILQLRPTTLEQHGLGPALAEYVKTVAERENWAVENEIDQALRLRGAEGEALFRVAQEALNNVAKHARASRIRVVLERAGEGEIRLLVADDGVGFDRRAGIRPTAVGLAGMHERMAALGGSIQVRSAPGEGTEVLAVLPPSPPRDEGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L70</accession>
    <name>Q67L70_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-seryl-tRNA(Sec) selenium transferase</fullName>
            <ecNumber evidence="5">2.9.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Selenocysteine synthase</fullName>
            <shortName evidence="5">Sec synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Selenocysteinyl-tRNA(Sec) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">selA</name>
        <name evidence="8" type="ordered locus">STH2591</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H(+) + L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22728"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:9743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:78573"/>
            <dbReference type="EC" id="2.9.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (bacterial route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SelA family.</text>
    </comment>
    <dbReference type="EC" id="2.9.1.1" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196713.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2591"/>
    <dbReference type="EnsemblBacteria" id="BAD41576">
        <property type="protein sequence ID" value="BAD41576"/>
        <property type="gene ID" value="STH2591"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2591"/>
    <dbReference type="eggNOG" id="COG1921">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038142_1_0_9"/>
    <dbReference type="OMA" id="GATNRTH"/>
    <dbReference type="OrthoDB" id="1124266at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00896"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004125">
        <property type="term" value="F:L-seryl-tRNASec selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00423">
        <property type="entry name" value="SelA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018319">
        <property type="entry name" value="SelA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004534">
        <property type="entry name" value="SelA_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025862">
        <property type="entry name" value="SelA_trans_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12390">
        <property type="entry name" value="Se-cys_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03841">
        <property type="entry name" value="SelA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00474">
        <property type="entry name" value="selA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0711">Selenium</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Se-cys_synth_N" evidence="7">
        <location>
            <begin position="9"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5 6">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR618319-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="466" mass="49832" checksum="CDE03B0C666D96D8" modified="2004-10-11" version="1">MKPWQQSALRAIPPVNEILETPTGQRLLAERARALVVDAVGEALDALRRKIAAAASEEELAVLDRSPEGLAADAAARLSRRLRPRLRRVINATGVVIHTNMGRAPLAEEAVAAIVDIAGRYNNLELDLSTGERGSRYAHVEALLAELTGAEAAMVVNNNAAAVLLMLSALAAGKEVVVSRGELVEIGGSFRVPDVMKQGGARLVEVGTTNKTHPRDYEEAIGPETCALLKVHRSNFRLEGFTAEVSVAELARIAHRHGLPCLVDWGSGVMLDLSRYGLPHEATMPELIAAGADLVTFSGDKLLGAPQGGFIVGRRDLVERCRRHPLTRAVRVDKLTLAGIEATLKLYLEPERAAERIPAVRMLTASREQIAPRAEALAGKLRAAGIACEVIDGVSRAGGGALPGVDLPTRLVAVAHPAPQEVEARLRAFDPPVMVRIQAGLLLLDPRTLWDDELDVVVSALRSAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC6</accession>
    <name>Q67LC6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarate hydratase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196658.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2535"/>
    <dbReference type="EnsemblBacteria" id="BAD41520">
        <property type="protein sequence ID" value="BAD41520"/>
        <property type="gene ID" value="STH2535"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2535"/>
    <dbReference type="eggNOG" id="COG1838">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098588_2_0_9"/>
    <dbReference type="OMA" id="PTSAYRM"/>
    <dbReference type="OrthoDB" id="1627779at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase_C" evidence="3">
        <location>
            <begin position="3"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="21436" checksum="5EE900786015FF8C" modified="2004-10-11" version="1">MARVHITTPVTPEQVRQIRAGDEVIITGEIYTARDAAHKRMVEDLAAGRPLPFDPEGAVIYYVGPTPPKPGQAIGSAGPTTSYRMDKYTPEILRHGVRLVIGKGYRGDEVKAALQEYGAAYLVATGGAGALLAKRIEEAEVVAYEDLGPEAVRRLRVREFPTICINDVYGGDQYFTGQSQWVKPDAMVKPKPPVASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN6</accession>
    <name>Q67LN6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196548.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2425"/>
    <dbReference type="EnsemblBacteria" id="BAD41410">
        <property type="protein sequence ID" value="BAD41410"/>
        <property type="gene ID" value="STH2425"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2425"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029256_0_0_9"/>
    <dbReference type="OMA" id="GQESIYN"/>
    <dbReference type="OrthoDB" id="1130951at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023995">
        <property type="entry name" value="HemZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932:SF1">
        <property type="entry name" value="PTHR13932:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00310">
        <property type="entry name" value="oxygen-independent_coproporphy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03994">
        <property type="entry name" value="rSAM_HemZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="187"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="526" mass="57410" checksum="5D653B842C2A7870" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR8</accession>
    <name>Q67LR8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2393"/>
    <dbReference type="EnsemblBacteria" id="BAD41378">
        <property type="protein sequence ID" value="BAD41378"/>
        <property type="gene ID" value="STH2393"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2393"/>
    <dbReference type="HOGENOM" id="CLU_1495465_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="20481" checksum="2082C923048D0E99" modified="2004-10-11" version="1">MRSQLAAAGLRERLEAEIYDFELELRGTDKSNLLIRLDSFRLEILGARPDLLLHNLAAIILTEAGAFRLNTVEVGFTAWLKAGQGRPLNLVAQAFTPAWGAAEAEFLDRRFAMTWDWATPTTGYTFHASSAEDEELMLNFKAREGYMTLTELQTGLWIQEQRQRFEQAARLFLNLLGWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX6</accession>
    <name>Q67LX6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2335</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2335"/>
    <dbReference type="EnsemblBacteria" id="BAD41320">
        <property type="protein sequence ID" value="BAD41320"/>
        <property type="gene ID" value="STH2335"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2335"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044700_0_0_9"/>
    <dbReference type="OMA" id="YADKLWY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="72"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="424" mass="47803" checksum="7EC47E4B496ED359" modified="2004-10-11" version="1">MVHNAPLESHREIAAKVKTYLDDESSNWHQLGMRMLTETHPNVRKRTAVNFFINASLVGIPKQREAEKRLGVKVPWAVLMDPTAKCNLRCIGCWAGDYELRPELSLETMDRICREGGELGIHFYVISGGEPMVRQNDLLELARRHPDKMFHIYSNGTLITREFAREAAERGNMVFALSLEGLEASTDARRGKGVFQKVMDAMDILREAGVIYGFSATYTRKNTEELGSEAFIDRMVEKGAAFGWFFTYIPIGRDVDLELMATPEQRAYMFDRITAFRRTKPIFLVDFWNDGEAAVGCIAGGRKYFHINSAGDVEPCAFAHYATCNIHEVSVAEALQNPLFKAYQKRQPFSGNLRRPCPIIDHPYVLRDMVKESGAYYTQKSDNETVDEFAEKLAGYAAAWGELADEIWEKRLAGAPAGMDGGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M21</accession>
    <name>Q67M21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2289</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196412.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41274">
        <property type="protein sequence ID" value="BAD41274"/>
        <property type="gene ID" value="STH2289"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2289"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_8_0_9"/>
    <dbReference type="OMA" id="QEAWIAG"/>
    <dbReference type="OrthoDB" id="792870at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="47985" checksum="F4A1A31D97B5D0E1" modified="2004-10-11" version="1">MRSRSNTSQRGNPQVTNFRIALEELLRKSGVDDVDFLREGVRVLAQGLMELEVSQQIGAERYERSSERSNYRNGYRERQWDTRVGTIDLQIPKLRKGSYMPSWLEPRRRAEKALVAVVQEAYIQGVSTRKVDELVQALGMTGVSKSQVSRLCAELDEVVEAFRNRPLEGRYPYVWLDAKYEKVRENGRVSSMALVIAMGVREDGEREILGLDVGPSEDGAFWTAFLRQLVARGLKGVLLVISDNHVGLREAIRTVFSGASWQRCRVHFMRNLLGYVPKNLQSMVSAAVRTIFAQPDQQAAKSQLAVVVENLRKQFPRAAQLLEDAEEDILAYMAFPTEHWRRLHSTNPLERLNREIGRRTEIVGIFPNREALIRLAGAVMIEQQEEWMTAPRRYFSQASMAKLYAHDPSLGRPELLPLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M28</accession>
    <name>Q67M28_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2282"/>
    <dbReference type="EnsemblBacteria" id="BAD41267">
        <property type="protein sequence ID" value="BAD41267"/>
        <property type="gene ID" value="STH2282"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2282"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_435987_0_0_9"/>
    <dbReference type="OMA" id="GDGFYWE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="622" mass="69929" checksum="A038E292E0B02E4A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M38</accession>
    <name>Q67M38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2272"/>
    <dbReference type="EnsemblBacteria" id="BAD41257">
        <property type="protein sequence ID" value="BAD41257"/>
        <property type="gene ID" value="STH2272"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2272"/>
    <dbReference type="HOGENOM" id="CLU_1453718_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269267" evidence="1">
        <location>
            <begin position="29"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="186" mass="19433" checksum="4259AC46D7A3DA7B" modified="2004-10-11" version="1">MSSSAVGGASLVKTAFSTCSPNSPHSSAAAFANLGSRSIPFAHSIRPFTRHGLPNCRCYGSTWTTNRVMHSARCGWASANRRSNAKNLTENGTPSNDLPSAAMACSTVRVTHPIPVARSVRSSGSKRRLCLDSSAVPYSYSAICQASGSTRTFTVEGSTGPGKSMSTTIGMAKRAAVHPGFTFQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M45</accession>
    <name>Q67M45_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2265"/>
    <dbReference type="EnsemblBacteria" id="BAD41250">
        <property type="protein sequence ID" value="BAD41250"/>
        <property type="gene ID" value="STH2265"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2265"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038364_0_0_9"/>
    <dbReference type="OMA" id="RIGYHAL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015378">
        <property type="entry name" value="Transposase-like_Mu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09299">
        <property type="entry name" value="Mu-transpos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="162"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="469" mass="54800" checksum="11A7A4E5885A982C" modified="2004-10-11" version="1">MAREGLTMDEQQRDEIAAFRWQLIAPVVAEDVTRLERRRILQQIARQHHQIPHSDKTRVSVCTLERWIAAYRQAGFDGLKPKVRDDARKGRVITPEILEHAIAARREVPERSVQQIIDTLEKAGVVPPGLLRRSTLSQHLVRAGYSRKAMLSRKRRATFRRFEAAHRNDCWQGDVCHLLNLPHPDRPDKTRKVYLIAFIDDYSRIVYGQMYWEEKLPRLEDCLKRTILRYGLPRRIYVDNGAIYSARHLARICGRLGIRLTHSRPYRPQGRGKIERFFLTVQKSFLPEAQQLIAAGQLATLEQLNELFWAWLEVAYHNRVHGVTKQTPRQRFESDTEPLRRIDPSALREVFLWEEERVVDQTGCFSLYRNQYEVDPALARRKVTLRYDPYDLSQIQVWHEGKRFADAVPLKLHRTRHRSVSPAPEPATAAPKTGLNFLTLAKKQHDTERRRRLGETAYTRLTQPKGGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI9</accession>
    <name>Q67MI9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2119"/>
    <dbReference type="EnsemblBacteria" id="BAD41104">
        <property type="protein sequence ID" value="BAD41104"/>
        <property type="gene ID" value="STH2119"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2119"/>
    <dbReference type="eggNOG" id="COG2364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083843_0_0_9"/>
    <dbReference type="OMA" id="APWDVLH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038750">
        <property type="entry name" value="YczE/YyaS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40078">
        <property type="entry name" value="PTHR40078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="21623" checksum="1F8F9562DF60366C" modified="2004-10-11" version="1">MLWYYVGLVITGLGYALVIAQGLGASPWDILHLGLTGQTGIPLGRVVQITGAFVILVDVLLGMRPNLGMVLNMLSLGPITQFLLERLAAPATLPGRWLMLVAGIVVSGIGTAFYASADLGAGPRDSLMIGLTRKLGLPVAIVKNGLDVMVSLIGWWLGGPLGAGTVAVAVGLGPSVQLGFHLASLLARLRPLHGIVRPVPLRSATARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN5</accession>
    <name>Q67MN5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41058">
        <property type="protein sequence ID" value="BAD41058"/>
        <property type="gene ID" value="STH2073"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2073"/>
    <dbReference type="HOGENOM" id="CLU_2669743_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="7800" checksum="B1A3790A580BD02E" modified="2004-10-11" version="1">MAVGRLPPDVEAARRRPHAATVRSAPACVAASEPHTTSHGRGAIRPFPALVTGERPRVAVLNPETGQAQDPTPCA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS9</accession>
    <name>RNH22_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII 2</fullName>
            <shortName evidence="1">RNase HII 2</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB2</name>
        <name type="ordered locus">STH2029</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196155.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MS9"/>
    <dbReference type="SMR" id="Q67MS9"/>
    <dbReference type="STRING" id="292459.STH2029"/>
    <dbReference type="EnsemblBacteria" id="BAD41014">
        <property type="protein sequence ID" value="BAD41014"/>
        <property type="gene ID" value="STH2029"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2029"/>
    <dbReference type="eggNOG" id="COG0164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_3_2_9"/>
    <dbReference type="OMA" id="YPTKLHL"/>
    <dbReference type="OrthoDB" id="1685277at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07182">
        <property type="entry name" value="RNase_HII_bacteria_HII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_B">
        <property type="entry name" value="RNase_HII_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022898">
        <property type="entry name" value="RNase_HII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF18">
        <property type="entry name" value="PTHR10954:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235777" description="Ribonuclease HII 2">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="15"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <sequence length="211" mass="23038" checksum="5E52FDFFCD72A12D" modified="2004-10-11" version="1">MADLRLERVLWRHGCQVIGVDEAGRGPLAGPVVAAACILPPDAALPGVDDSKRMTEKRREAAFAAIQAVAVGWGIGIVDAARIDEINILQATFEAMAQAVEGARAMAAARPGRTAEAALLVDGNRPLPLWPGWQRTVVGGDHKSLSIAAASILAKVTRDRMMIEYDAHYPEYGFARNKGYGSREHWDALERYGPCPLHRRTFIGPRQIRFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW4</accession>
    <name>YQGF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1994</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YqgF nuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196121.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MW4"/>
    <dbReference type="SMR" id="Q67MW4"/>
    <dbReference type="STRING" id="292459.STH1994"/>
    <dbReference type="EnsemblBacteria" id="BAD40979">
        <property type="protein sequence ID" value="BAD40979"/>
        <property type="gene ID" value="STH1994"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1994"/>
    <dbReference type="eggNOG" id="COG0816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098240_2_0_9"/>
    <dbReference type="OMA" id="PMGWTAQ"/>
    <dbReference type="OrthoDB" id="2075502at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000172157" description="Putative pre-16S rRNA nuclease">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <sequence length="144" mass="15997" checksum="07E32E7602DCBE82" modified="2004-10-11" version="1">MRIGGLDVGEKTIGVALSDELGLTAQALEVIRRRSLEQDLARLDEIARHYGVVRWVVGMPRNMNGTYGPRAELTRAFMERLAGHSGLPVEAWDERLSTMAAERVLLEADVSRARRRKVIDKMAAAVILQGYLDARAARPPESFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ4</accession>
    <name>Q67MZ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40949">
        <property type="protein sequence ID" value="BAD40949"/>
        <property type="gene ID" value="STH1964"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1964"/>
    <dbReference type="HOGENOM" id="CLU_3391764_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="32" mass="3654" checksum="0E907B792D957853" modified="2004-10-11" version="1">MTDALKALEAVHDRYLTIMERAYRTGETDVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N29</accession>
    <name>Q67N29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1929</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196056.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1929"/>
    <dbReference type="EnsemblBacteria" id="BAD40914">
        <property type="protein sequence ID" value="BAD40914"/>
        <property type="gene ID" value="STH1929"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1929"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_9"/>
    <dbReference type="OMA" id="WQGINLE"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269286" evidence="2">
        <location>
            <begin position="27"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="87"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="338" mass="36169" checksum="ACC302618805DC06" modified="2004-10-11" version="1">MKKRSLAALMLSLLLVVGILTGCAGRQTAVNPDSPPAAPAAESSDQPADRAAGTGSAGQAEPRQTAYPLTITDGAGRQVTIAAEPRRVISVAPSNTELMFALGKGDLLVGRSDWDDYPPEAQEIESIGGFYSPDYEKIISLEPDLLLLTSGSVEAREKLENDYGLTTFVLNPSNFEELYEGILALGQVVNAQEAAEALVADMQREVEAIAGKVALAENRPVVFYQVWHDPITTAGPGSFIDDMIRIAGGTNAASFAGEPWPVISLEELVSADPDIIVTASEAAAREVRERPGWESIAAVKEGRVLGLPDENIVVRPGPRLIQGLQWFARNLHPELFEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N48</accession>
    <name>Q67N48_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1910</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1910"/>
    <dbReference type="REBASE" id="10272">
        <property type="entry name" value="M.StpORF1910P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40895">
        <property type="protein sequence ID" value="BAD40895"/>
        <property type="gene ID" value="STH1910"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1910"/>
    <dbReference type="eggNOG" id="COG0286">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013049_4_1_9"/>
    <dbReference type="OMA" id="FFAPIFE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HsdM_N" evidence="5">
        <location>
            <begin position="21"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="N6_Mtase" evidence="4">
        <location>
            <begin position="174"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="491"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="537" mass="60722" checksum="90AA6624BD68CE7D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N80</accession>
    <name>Q67N80_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4015 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40863">
        <property type="protein sequence ID" value="BAD40863"/>
        <property type="gene ID" value="STH1878"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1878"/>
    <dbReference type="eggNOG" id="COG1306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030168_0_0_9"/>
    <dbReference type="OMA" id="RPWIQDF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025275">
        <property type="entry name" value="DUF4015"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13200">
        <property type="entry name" value="DUF4015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4015" evidence="2">
        <location>
            <begin position="74"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="384" mass="41745" checksum="43A234864AAE1320" modified="2004-10-11" version="1">MPVGNITGPGGQDPGGTTDPGADDPGTAAPGGATDPGASADPGGSGNAQAPENGPQPGDGPARPAREMPDPVRGLHLSGWYAGSPDLVWPLLDWAKEAGINTIVLDLKAEDGYLSWESDLPLAQEIGANMRKIADLPAFVAEAHERGFWVAGRIVVMNDQWLYKARPEWAIPGFDGGAYSFMDPANENVWKYNVDVAKEAIAAGVDEIQFDYIRYSEHLREGYNGKDTTAEQRTKPINDFLRYAMAELKPLGVVVGADVFGLTTSVAEGDDMEIGQDYRQIAEIVDYIAPMVYPSHYAPYTYGLDNPNAHPYETVYNSMKKALERTEGLPIEKHRPWIQDFSLGGITYGAAEVMAQVQALKDLGIESFMLWDPSNKYSRDVSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N97</accession>
    <name>Q67N97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage III sporulation protein AB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoIIIAB</name>
        <name evidence="1" type="ordered locus">STH1861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195989.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1861"/>
    <dbReference type="EnsemblBacteria" id="BAD40846">
        <property type="protein sequence ID" value="BAD40846"/>
        <property type="gene ID" value="STH1861"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1861"/>
    <dbReference type="eggNOG" id="ENOG5032S0Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120887_1_2_9"/>
    <dbReference type="OMA" id="AKMWSYL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014198">
        <property type="entry name" value="Spore_III_AB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09548">
        <property type="entry name" value="Spore_III_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021435">
        <property type="entry name" value="SpoIIIAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02833">
        <property type="entry name" value="spore_III_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="18288" checksum="8C8433EFF306901C" modified="2004-10-11" version="1">MTLKLLGAALTVLAPSWIGFQIAARYARRPAELRGFQNGLAVLVTEVEYGATPMPDALRSAARASGPVAGAVLADAADRLEGGGGITPGEALAAALEERRGATCLTPADQEILAALVPVLGASDRRDQVRHLRLALERLAAAEAEATDERRRYEKMYRYVGVLSGLALVLILI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB6</accession>
    <name>DXS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="1">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="1">DXP synthase</shortName>
            <shortName evidence="1">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxs</name>
        <name type="ordered locus">STH1842</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195970.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NB6"/>
    <dbReference type="SMR" id="Q67NB6"/>
    <dbReference type="STRING" id="292459.STH1842"/>
    <dbReference type="EnsemblBacteria" id="BAD40827">
        <property type="protein sequence ID" value="BAD40827"/>
        <property type="gene ID" value="STH1842"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1842"/>
    <dbReference type="eggNOG" id="COG1154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_4_9"/>
    <dbReference type="OMA" id="QVGYHAQ"/>
    <dbReference type="OrthoDB" id="566385at2"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000256490" description="1-deoxy-D-xylulose-5-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <sequence length="648" mass="69789" checksum="236B8497DA0A6D00" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC6</accession>
    <name>Q67NC6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 8">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">spo0A1</name>
        <name evidence="12" type="ordered locus">STH1832</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8 9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195960.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1832"/>
    <dbReference type="EnsemblBacteria" id="BAD40817">
        <property type="protein sequence ID" value="BAD40817"/>
        <property type="gene ID" value="STH1832"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1832"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072509_0_0_9"/>
    <dbReference type="OMA" id="TKELYPM"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051606">
        <property type="term" value="P:detection of stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042173">
        <property type="term" value="P:regulation of sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014879">
        <property type="entry name" value="Spo0A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012052">
        <property type="entry name" value="Spore_0_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08769">
        <property type="entry name" value="Spo0A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002937">
        <property type="entry name" value="Res_reg_Spo0A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02875">
        <property type="entry name" value="spore_0_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 8" id="KW-0749">Sporulation</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="11">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="28221" checksum="3680E84FB60C9C99" modified="2004-10-11" version="1">MSAHKVRLLVCDDNRDFCALLQEYFALQPDMEVVGVAHNGLDAVAAIQEQQPDVVLLDIIMPYMDGIGVLEQLARTKLSARPRILMLSAFGQEAIARRVMAMGADYYVVKPVDLEILAARVRQLAWGDALADSPRVPPASHAAGHTLDEVHALLRALGIPPHLKGYRYLGDAILLVLEQPELLGAVTKGLYPAVARSHGTTASRVERAIRHAVGMAWDRGLEPLRKLFRRGAEGRRTKPSNSEFIAAVVDRLRAGSAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI1</accession>
    <name>Q67NI1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1777"/>
    <dbReference type="EnsemblBacteria" id="BAD40762">
        <property type="protein sequence ID" value="BAD40762"/>
        <property type="gene ID" value="STH1777"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1777"/>
    <dbReference type="eggNOG" id="COG1692">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068238_0_0_9"/>
    <dbReference type="OMA" id="NHIWDKK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005235">
        <property type="entry name" value="YmdB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36303">
        <property type="entry name" value="PTHR36303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13277">
        <property type="entry name" value="YmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004789">
        <property type="entry name" value="DR1281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00282">
        <property type="entry name" value="TIGR00282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004789-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="261" mass="28115" checksum="A25A4486FC660C9B" modified="2004-10-11" version="1">MGREVVARRLTPLVKETGADAVIVNGENAAGGKGISAQICQELFSLGVDVITLGNHAFDKREIATYIDGEPRLIRPLNYPPGTPGAGSYIVRRGGRPLLAVVQVQGRVYSPQLLEDPFRAVEGEVERLRQSTPVIIVDCHAEATSEKQAMGWFLDGQVSAVIGTHTHVQTADEVILPGGTAYMTDVGMCGPWVSVIGADRELVLERFLTQRPVRLEAARGPAILSAVLIEVDEATGKATGIQRILEREVATEPLASLFGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ0</accession>
    <name>Q67NJ0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4129 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1768"/>
    <dbReference type="EnsemblBacteria" id="BAD40753">
        <property type="protein sequence ID" value="BAD40753"/>
        <property type="gene ID" value="STH1768"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1768"/>
    <dbReference type="HOGENOM" id="CLU_611012_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="DUF4129"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4129" evidence="2">
        <location>
            <begin position="370"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="448" mass="47387" checksum="2F46E294A1D2594C" modified="2004-10-11" version="1">MAALVETAAALPWMLFLYAADGEAGWFEAVPGAWLPLAVFLTAAVWESGSRGDARPARVGALVAGAALAYLAAYALLPAPLRTGVFGLNPALAFVPVAVYLWYAGASCALEGLEYGRLFERAWRLFGGQLAGIVCLLLLGEARSGPVQLVLGWSVVLLFGAGLSLLILTRERALLGAFDAEEQAAEPTSPAVTGFVLALLAGTVAASAVLTVDRVATLLRAVGRLVAPVYNVLVQVAAYLAMGVAMLLEPLFNLLKWLASQQEPTEQGGGEDVAPGPPDLPPDAGAQLDLEPVLKAGLILVAVALAALLLWRMSAVRRRTSEVDEERTNLGFWASLWQDLRGLLQARRTAGAVGDGAGEEPVPPGSARELYRRLQRWGSSRVRPRRPAETPNAYRSALAGAQPRAADAVDAVTAVYNQERYGSRPPDAETVAEAAALWEASCERDFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP5</accession>
    <name>Q67NP5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3'-5' exonuclease DinG</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dinG</name>
        <name evidence="8" type="ordered locus">STH1713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">3'-5' exonuclease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family. DinG subfamily. Type 2 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1713"/>
    <dbReference type="EnsemblBacteria" id="BAD40698">
        <property type="protein sequence ID" value="BAD40698"/>
        <property type="gene ID" value="STH1713"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1713"/>
    <dbReference type="eggNOG" id="COG1199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012117_1_0_9"/>
    <dbReference type="OMA" id="VVTNHAM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02206">
        <property type="entry name" value="DinG_exonucl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006310">
        <property type="entry name" value="DinG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="251"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="766"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box" evidence="4">
        <location>
            <begin position="472"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="286"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="958" mass="105555" checksum="1BBE3BBCF3AD0417" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS8</accession>
    <name>PLSY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">G3P acyltransferase</fullName>
            <shortName evidence="1">GPAT</shortName>
            <ecNumber evidence="1">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="1">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsY</name>
        <name type="ordered locus">STH1680</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsY family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195808.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NS8"/>
    <dbReference type="SMR" id="Q67NS8"/>
    <dbReference type="STRING" id="292459.STH1680"/>
    <dbReference type="PRIDE" id="Q67NS8"/>
    <dbReference type="EnsemblBacteria" id="BAD40665">
        <property type="protein sequence ID" value="BAD40665"/>
        <property type="gene ID" value="STH1680"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1680"/>
    <dbReference type="eggNOG" id="COG0344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081254_4_0_9"/>
    <dbReference type="OMA" id="ILGHNWP"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00023">
        <property type="entry name" value="TIGR00023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000188473" description="Glycerol-3-phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <sequence length="199" mass="20940" checksum="21541824EDE7047A" modified="2004-10-11" version="1">MAELVSVAILGYLLGSIPVGFLMGKLRGIDVRRYGSGATGGTNVLRTLGPWAALFTVLCDIGKGLLAAYLGERLAGEWGFVAAGLLASLGHSYPVWLRFRGGKSVATSGGVMLLHYPLAVLVGIAAGALAVVPTRWVSLGSLTASLAVVLQLFLLDAPLSHRLLVVALAVVIYVRHWENMKRIAAGTENRLGVKARPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT2</accession>
    <name>Q67NT2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1676</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195804.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1676"/>
    <dbReference type="EnsemblBacteria" id="BAD40661">
        <property type="protein sequence ID" value="BAD40661"/>
        <property type="gene ID" value="STH1676"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1676"/>
    <dbReference type="HOGENOM" id="CLU_1776516_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="15416" checksum="084A04412F7AD005" modified="2004-10-11" version="1">MGVRPTRDALRGRAPAPAGRRRRVAMLLSALAAVFSAQVLLTGCSARPLPVQPADAVAAALAHPEVADWYAAHSAPRVLAGLNPAAARGLQRFRPDALVDLAAEGLVVRFTSALGEPPKRVDVLIDKDSGQVLDVRMGGVGWRGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY3</accession>
    <name>Q67NY3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative zinc/cadmium-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1625</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195754.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1625"/>
    <dbReference type="EnsemblBacteria" id="BAD40610">
        <property type="protein sequence ID" value="BAD40610"/>
        <property type="gene ID" value="STH1625"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1625"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_2_9"/>
    <dbReference type="OMA" id="FAQPWET"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="682"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="4"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="710" mass="74129" checksum="F71D52C003C5E5AC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P29</accession>
    <name>Q67P29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative propionyl-CoA carboxylase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195708.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1579"/>
    <dbReference type="EnsemblBacteria" id="BAD40564">
        <property type="protein sequence ID" value="BAD40564"/>
        <property type="gene ID" value="STH1579"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1579"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_6_1_9"/>
    <dbReference type="OMA" id="AYLPIMS"/>
    <dbReference type="OrthoDB" id="886663at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="PTHR22855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="2">
        <location>
            <begin position="257"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="508" mass="55660" checksum="51FE89288573868B" modified="2004-10-11" version="1">MSLDAELKAREARIKQGGAPKYHEQARAKGKLFARERIRLLLDDGSLVEDGLFANCEAPDLPADGVVTGLGRIDGRPVAVMANDSTVKAGSWGARTVEKILRIQETAERLRIPIFYLVDSAGARITDQVEMFPGRRHAGRIFYNEVRLSGLVPQICLLFGPSAAGGAYIPAFCDVVFMVRGNASMYLGSPRMAEMVIGEKVTLEEMGGAEMHTAVSGCGDVLCETEEEAIQLARRYLSYFPQRTGERPAPAPPAEPKTGRPLAEIVPVNQNAAFDMYELIDRIIDEGSWFEIKKRFAPELITGLARIGGRVVGIVANQPRVKGGVLFVDSADKAARFIWLCDAFEIPLLFLADVPGFMIGTKVERQGIIRHGAKFIAAVSEASVPKICLVVRKAYGAGLYAMAGPAFEPDCTLALPTASIAVMGPEAAVNAVYANKIAELPPEERPAYVARLREEYQKDIDIYRLASELIVDGIIPFERVREELLARFALYETRQQNWPAKKRPVTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P52</accession>
    <name>Q67P52_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease J</fullName>
            <shortName evidence="9">RNase J</shortName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnj</name>
        <name evidence="12" type="ordered locus">STH1556</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, may be a subunit of the RNA degradosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1556"/>
    <dbReference type="EnsemblBacteria" id="BAD40541">
        <property type="protein sequence ID" value="BAD40541"/>
        <property type="gene ID" value="STH1556"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1556"/>
    <dbReference type="eggNOG" id="COG0595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008727_3_2_9"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="OrthoDB" id="580619at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01491">
        <property type="entry name" value="RNase_J_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030854">
        <property type="entry name" value="RNase_J_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041636">
        <property type="entry name" value="RNase_J_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001587">
        <property type="entry name" value="RNase_J_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17770">
        <property type="entry name" value="RNase_J_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004803">
        <property type="entry name" value="RnjA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01292">
        <property type="entry name" value="UPF0036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="11">
        <location>
            <begin position="23"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9 10">
        <location>
            <begin position="367"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR004803-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="558" mass="61518" checksum="5C93D9BEF0FAC3D6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P79</accession>
    <name>Q67P79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195659.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1529"/>
    <dbReference type="EnsemblBacteria" id="BAD40514">
        <property type="protein sequence ID" value="BAD40514"/>
        <property type="gene ID" value="STH1529"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1529"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_1_9"/>
    <dbReference type="OMA" id="YRTISEN"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="105"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="18492" checksum="BA9C3B45EA17253F" modified="2004-10-11" version="1">MARTEAERREAFAALFSQMAPRLYRTAVGILGNPHDAADALQEAGLKAYRYFDSLADPAAGPAWLNRILINTCYDVGRSRSRAVPTGLEVVECPEGYAAPETDWQLLEALKLLPPEQRATVVLRFFQDLTIPQIAQVMAVPEGTVKSRLHASLAKLRAALASSEKEGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P88</accession>
    <name>Q67P88_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YlxR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1520"/>
    <dbReference type="EnsemblBacteria" id="BAD40505">
        <property type="protein sequence ID" value="BAD40505"/>
        <property type="gene ID" value="STH1520"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1520"/>
    <dbReference type="eggNOG" id="COG2740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147970_2_1_9"/>
    <dbReference type="OMA" id="CVGCQEM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00279">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007393">
        <property type="entry name" value="DUF448"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037465">
        <property type="entry name" value="YlxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035931">
        <property type="entry name" value="YlxR-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34215">
        <property type="entry name" value="PTHR34215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04296">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64376">
        <property type="entry name" value="SSF64376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YlxR" evidence="1">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="10079" checksum="49FBF4E832BE2D94" modified="2004-10-11" version="1">MKKVPQRMCVGCGQMRPKKELIRIVRTPEGAIELDTSPSGKRPGRGAYLCPDSQCLAKAVKGKRLERSLEQPISDEVWTQLEAQLGTVKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL0</accession>
    <name>Q67PL0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1398"/>
    <dbReference type="EnsemblBacteria" id="BAD40383">
        <property type="protein sequence ID" value="BAD40383"/>
        <property type="gene ID" value="STH1398"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1398"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2385053_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="94" mass="10319" checksum="45F40DAF8CA73CE0" modified="2004-10-11" version="1">MRPSDLRVSIVGDRQVVLEGEVAYRHPLPRENLALNECTYGPFSRTVHLPLPVDAAGVAVNLQDGVLTVDMRVRAEAVHLHWQPKEAGSGERPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL3</accession>
    <name>Q67PL3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195525.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1395"/>
    <dbReference type="EnsemblBacteria" id="BAD40380">
        <property type="protein sequence ID" value="BAD40380"/>
        <property type="gene ID" value="STH1395"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1395"/>
    <dbReference type="HOGENOM" id="CLU_2669741_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="7764" checksum="5CB55D2E744D7FAE" modified="2004-10-11" version="1">MDRLNIGAVKVTNVSGVVSTGFAYIRRSRTSGKGTVGGSAITGDCGIAPLYFGVLVDSDAVDAPFWWTVNGWGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ6</accession>
    <name>Q67PZ6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxxC_CXXC_SSSS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195393.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1262"/>
    <dbReference type="EnsemblBacteria" id="BAD40247">
        <property type="protein sequence ID" value="BAD40247"/>
        <property type="gene ID" value="STH1262"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1262"/>
    <dbReference type="eggNOG" id="COG2331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136025_4_0_9"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013429">
        <property type="entry name" value="Regulatory_FmdB_Zinc_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09723">
        <property type="entry name" value="Zn-ribbon_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00834">
        <property type="entry name" value="CxxC_CXXC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02605">
        <property type="entry name" value="CxxC_CxxC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC_CXXC_SSSS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="9620" checksum="97765D9A572EF927" modified="2004-10-11" version="1">MPIYEFRCNACNHLFEELVPLNTTGENQKCPECGHVGARRLVSAFAAHGLENGHIAVGQKLTSLDKARASGKSESKSGESKSSGESASKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q11</accession>
    <name>Q67Q11_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PTS system N-acetylgalactosamine-specific IID component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">agaD</name>
        <name evidence="10" type="ordered locus">STH1247</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195378.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1247"/>
    <dbReference type="EnsemblBacteria" id="BAD40232">
        <property type="protein sequence ID" value="BAD40232"/>
        <property type="gene ID" value="STH1247"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1247"/>
    <dbReference type="eggNOG" id="COG3716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060742_1_0_9"/>
    <dbReference type="OMA" id="YNEYDEV"/>
    <dbReference type="OrthoDB" id="1632067at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004704">
        <property type="entry name" value="PTS_IID_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03613">
        <property type="entry name" value="EIID-AGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51108">
        <property type="entry name" value="PTS_EIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="37979" checksum="71E203FE32783999" modified="2004-10-11" version="1">MAYSIPDQYRDRTPAPPLDRATLNQMAWRSVFLQASFNYERMQAAGWLYAILPGLQKIHRNKDDLAAAMSHNLEFFNTHPFLVTFVMGIVLSLEQNKADIATIRAVRVSAMGPLGGIGDALFWFTLVPITAGITSNMALTGNIAAPFVFLLVFNVAQFAIRYWLMHWSYRLGTDAIGILTANARQFTRAASILGVFVVGALTCLYGGTAVNVQIPNGTTFEAVPITAVVSADQLDRYRDLLYQTDQNGNLIRGADGQPQLAEGASIRPLASGDFLITYNEYDEVPVTIDVQEILNGILPKLIPLGLTLLLYFLFTKRNWTPLTAIGFLLVLGLIGSGFGIWPSIW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q46</accession>
    <name>MURA1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1</fullName>
            <ecNumber evidence="1">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enoylpyruvate transferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylglucosamine enolpyruvyl transferase 1</fullName>
            <shortName evidence="1">EPT 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murA1</name>
        <name type="ordered locus">STH1212</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q46"/>
    <dbReference type="SMR" id="Q67Q46"/>
    <dbReference type="STRING" id="292459.STH1212"/>
    <dbReference type="PRIDE" id="Q67Q46"/>
    <dbReference type="EnsemblBacteria" id="BAD40197">
        <property type="protein sequence ID" value="BAD40197"/>
        <property type="gene ID" value="STH1212"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1212"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_9"/>
    <dbReference type="OMA" id="RYVITGK"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231287" description="UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <sequence length="441" mass="47251" checksum="A6E5EF105C7FB604" modified="2004-10-11" version="1">MDRPAGLRLRPREVACIVKPNERYVVAGGCRLQGEVPVSGAKNAALPILAAALLTSGESVIHNAPMIRDVAVMIEILHKLGARVEEGPEGVSGRTLRIRADGLNGQEVGADFTREMRSSIFLMGPLLARVGKIRISYPGGCAIGPRPIDFHLRGLEALGARIEERFGYIEASVPRRLRGTEIYLDFPSVGATENLMMAAVLAEGTTVIRNAAREPEIVDLQAFLNKMGARVRGAGLDVIRIDGVSKLGSVEHTVIPDRIEAATFLAAAAITGGEVTVQGVIPEHVDAVTAKLREMGTTIREYGTAVTAVGPRRLKAADVKTLPYPGFPTDMQPQMMALAATADGTSIITETIFENRFKVADELRRMGANIKTEGRTAVVQGVPSLSGATVVCPALREGMALILAGLRAEGETTIEDIYHIDRGYQHLEQKLTRLGATISRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q56</accession>
    <name>Q67Q56_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1202</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195333.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1202"/>
    <dbReference type="EnsemblBacteria" id="BAD40187">
        <property type="protein sequence ID" value="BAD40187"/>
        <property type="gene ID" value="STH1202"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1202"/>
    <dbReference type="HOGENOM" id="CLU_1585639_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="63"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="168" mass="17918" checksum="5C62124699227A53" modified="2004-10-11" version="1">MSAAPQWNRVPKVQPAPQVAPAPRPVPRPARPRVSIPAVLGLAGAWALLIFLAFAVVQTRMEIRAVEAATAQAQRQIAHLQEQNRALEAMIANAASVEEVQRWALAHGMRPPAGVDGTLEGRAEAVAVRTPAQPAAAEPVATEEAPTLWQALRDRFTERVGALAAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q66</accession>
    <name>Q67Q66_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="3">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = 5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1192"/>
    <dbReference type="EnsemblBacteria" id="BAD40177">
        <property type="protein sequence ID" value="BAD40177"/>
        <property type="gene ID" value="STH1192"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1192"/>
    <dbReference type="eggNOG" id="COG0212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066245_1_1_9"/>
    <dbReference type="OMA" id="MPLVGFD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="PTHR23407:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="135"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="21607" checksum="19CE2D062D425FCC" modified="2004-10-11" version="1">MDLSKQELRRRMIAARQALDPAERARLSGRAQRAVLAAPEWARARTVLLYIPVRGEVDTAALAAAGRQAGKRLLLPRVERAERGLRLHRWDGTPEQLVRGAYGIPEPRPDLPQEDPRAVDLVVVPGVAFDRRGRRLGYGGGYYDRLLPQLARAAAIGLGYGFQLVERLPAEAHDVPLDALATDLGLTRFPASADPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q71</accession>
    <name>Q67Q71_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA mutase, C-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195318.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1187"/>
    <dbReference type="EnsemblBacteria" id="BAD40172">
        <property type="protein sequence ID" value="BAD40172"/>
        <property type="gene ID" value="STH1187"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1187"/>
    <dbReference type="eggNOG" id="COG2185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128233_0_0_9"/>
    <dbReference type="OMA" id="VYTGLHQ"/>
    <dbReference type="OrthoDB" id="1335734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="3">
        <location>
            <begin position="3"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="131" mass="13842" checksum="BF0175958E16DE83" modified="2004-10-11" version="1">MRTIRVLVAKPGLDGHDRGAKVVARAFRDAGFEVIYTGLRQTPEQIAEAALQEDVDVVALSILSGAHNTLLPRTVAALRARGLDDVLVLAGGVIPAEDIPALKAAGIAEVFTPGTPTRAAVEFIRQRLQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG0</accession>
    <name>Q67QG0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group 1 family glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1098"/>
    <dbReference type="EnsemblBacteria" id="BAD40083">
        <property type="protein sequence ID" value="BAD40083"/>
        <property type="gene ID" value="STH1098"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1098"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_9"/>
    <dbReference type="OMA" id="PHELAMG"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="1">
        <location>
            <begin position="14"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="372" mass="40355" checksum="6FBCAE7AEC25D9C4" modified="2004-10-11" version="1">MPNILLAAMALDLGGAETHVISLARELHRRGHRVVVASAGGRMVPLLEERGIPHIVVPMASRNPLAMARAYRRLRRLIAEHQIGLIHAHARIPAWLCALAVRGKGIPLVTTYHGVYNAGFPWKYLTTFGQRAIAVSEDVRRHLEGPLGAPPAIIRVIPNGFETGEFRPGLDIAPLLRELGVDRTGPHVVHASRLTDEFADTAVALLQALPRLNEQFPTIRLWILGDGNRYAEVARLADEVNRRLGREAARAVGHRLDVPAFFNLADCVVAVARTATEAMLCERPVIIAGEGGYRGILTPERIPLYAGSNFTAREGGTPLNPAVLAADIAGLLAPGNEAERQRLGRAGREFVVASLSIESVTARILDVYAEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI9</accession>
    <name>Q67QI9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195201.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1069"/>
    <dbReference type="EnsemblBacteria" id="BAD40054">
        <property type="protein sequence ID" value="BAD40054"/>
        <property type="gene ID" value="STH1069"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1069"/>
    <dbReference type="HOGENOM" id="CLU_1239614_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="SSF55724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269329" evidence="2">
        <location>
            <begin position="28"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="24343" checksum="BC28CD0E3A3C0BCF" modified="2004-10-11" version="1">MRRRLRLLAPLLAILLLTAAGCGEPEAQGPAAGGDQTDVQSEPPAEGEGQQDGQQIPLTLVYSHPDTGLTLGVPEGWVVYPLEDAVVALISPERGEEDFFRENILVTADGQFPDPALDVYVEALEQEVRHRYPDTETLESGEATVGGVPARWIVDRFTGEKGETRVYRVVLVRDGIAYVLHGTAPVWTFDDYRPVFEAVAQSITWVEPRAVEPEPEAPSETGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ2</accession>
    <name>Q67QJ2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT</fullName>
            <ecNumber evidence="1">6.3.5.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murT</name>
        <name evidence="5" type="ordered locus">STH1066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:59488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with GatD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurT subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.13" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1066"/>
    <dbReference type="EnsemblBacteria" id="BAD40051">
        <property type="protein sequence ID" value="BAD40051"/>
        <property type="gene ID" value="STH1066"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1066"/>
    <dbReference type="eggNOG" id="COG0769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041534_0_0_9"/>
    <dbReference type="OMA" id="WLWDVDY"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140282">
        <property type="term" value="F:carbon-nitrogen ligase activity on lipid II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02214">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043703">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013564">
        <property type="entry name" value="MurT_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08353">
        <property type="entry name" value="MurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269938" description="Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT" evidence="2">
        <location>
            <begin position="24"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="3">
        <location>
            <begin position="56"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="MurT_C" evidence="4">
        <location>
            <begin position="331"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="464" mass="50713" checksum="D4F8AEA51F755B02" modified="2004-10-11" version="1">MNLRSRMAVWMGKALVTATRVAGRGGTSLPGAVARKVAPDLLRTLAAQPREGVVAITGTNGKTTTAKMLASICEAAGWDVAHNRAGANLISGVTTAFIQAADWKGRIHRSVGLLEIDEATMPRACQEVRPKVAVVTNFFRDQLDRFGELEHTVDLVARGLAHLAPGGRAVLNADDPNCARLGQRRGVEPLYYGLQVDAATAEAASQAADARHCVQCGHPYEYRVLHYAHLGDYRCPQCGHRRPEPRVYVDRLWDVDARGSSMRLVTPEGACEVRLQIPGLYNVYNAIAAAAGAVALGLPLAAIRQGLETTTSQFGRMEMITIGDRQVFMALVKNPAGFDSVIQTVMQAEARKNLVIAINDQYADGTDVSWLWDVDFERLAGHQSEINFVICTGVRAEDMAVRLKYAGMDPAKLSVEKDPERAVKQGLRYIQPGDLLYILPTYTAMLQIRGLIARKGHVKKYWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK9</accession>
    <name>Q67QK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1049"/>
    <dbReference type="EnsemblBacteria" id="BAD40034">
        <property type="protein sequence ID" value="BAD40034"/>
        <property type="gene ID" value="STH1049"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1049"/>
    <dbReference type="HOGENOM" id="CLU_2083669_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012851">
        <property type="entry name" value="Spore_coat_CotF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07875">
        <property type="entry name" value="Coat_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="117" mass="13120" checksum="ED43A9A38E9BE652" modified="2004-10-11" version="1">MTDADRLRDCLCSSRDLAKLYAEAALEAANNGVREFFLAMHGEETHNQEVLFHFLHTRGEHPTRAADPGHIAAVRQRYGEAYRALGLTDPPSFRRYATADPRVPPAAAQAPEHFRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL1</accession>
    <name>Q67QL1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 H(+) + 2 superoxide = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195179.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1047"/>
    <dbReference type="EnsemblBacteria" id="BAD40032">
        <property type="protein sequence ID" value="BAD40032"/>
        <property type="gene ID" value="STH1047"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1047"/>
    <dbReference type="eggNOG" id="COG0605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_0_1_9"/>
    <dbReference type="OMA" id="KWGSFDK"/>
    <dbReference type="OrthoDB" id="1440645at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="7">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="8">
        <location>
            <begin position="97"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="204" mass="22633" checksum="E1B80CED86FB0CA2" modified="2004-10-11" version="1">MAFQLPALPYPTNALEPYIDAQTMEIHHGKHHAAYVNNLNAALEGHPELQSKSIEELLRGIDSVPESIRTAVRNNGGGHANHTLYWEIMTPGGAKEPGGELAEAINAAFGSFQNFKDEFAKAGAGRFGSGWAWLVVTKDGKLAVYSTANQDSPLMQGDTPILGMDVWEHAYYLKYQNRRPEYIQAFMNVINWDKVAERYAAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM2</accession>
    <name>Q67QM2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1036"/>
    <dbReference type="EnsemblBacteria" id="BAD40021">
        <property type="protein sequence ID" value="BAD40021"/>
        <property type="gene ID" value="STH1036"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1036"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_0_9"/>
    <dbReference type="OMA" id="VLMVPHA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="103"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="307" mass="33452" checksum="B2EAAC7D96D24786" modified="2004-10-11" version="1">MAISRPTPDPIPTQFDGLTAQETSYARLVLRRFLRNRMAVAGATILLILILISAFAPVITQATLGWGRDEIDIQYIRAAPSARHPLGTDSAGRDNLTRLLYGGRVSLSIALGSVIVYMVLGIVIGSVAGYFGGWIDNVLMRLVDVIQSFPFLMFALTIVAIRGPSVSNLVFAIVFLSWPVPARLVRGEFLSLRERDFIEAARAAGVGTFRMIVRHLLPNAMAPLIVNATLEVAGIILAEAGLSYLGFGVNQPVPTWGNMLSEANNMAVLQTRPWTWIPPGLMIFLTVLSINFIGDGLRDALDPRLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU9</accession>
    <name>Q67QU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative Zn-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH959</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195091.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH959"/>
    <dbReference type="EnsemblBacteria" id="BAD39944">
        <property type="protein sequence ID" value="BAD39944"/>
        <property type="gene ID" value="STH959"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH959"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025947_1_1_9"/>
    <dbReference type="OMA" id="LPFKIYK"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 8 12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48_N" evidence="11">
        <location>
            <begin position="92"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="10">
        <location>
            <begin position="211"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="45078" checksum="E13E1C331954FAE4" modified="2004-10-11" version="1">MREPTLPLKGFWTLLLAVGMGLILLLLFATLEPRPLDADALNYFPASLVEEGRRFAREARVAASLRALASLGALAWLCFHPAGARLLARLEARGGDRWWRQVAWVAVGIGLVTAAVELPFAYYLGHVHERAYGLTRRSGLSWLGDHLLGLALDTVVSTLLWLALYALIRRSPRRWWAGAGLITAAYSLLLAVLYPVLLLPLFHQVRPVYDPQVLGMIRALAERAGVRVEKVAEVRVGHESSRINAMVAGIGPTKQVLLYDTLLRELTPAEVEAVLAHELSHAVHGDLFRGWALQAALDAASMGLAAWMLCAMRGVAPLGLPGPHAPRGIALLMLFFSLCNVAGGPAQAALSRRAEVRADRYALELTQNPRALVQSFQKLARANPGDVAPPPLVEFLSYSHPSIMRRIRAAEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV5</accession>
    <name>Q67QV5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PTS system cellobiose-specific IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per trimer.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195085.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH953"/>
    <dbReference type="EnsemblBacteria" id="BAD39938">
        <property type="protein sequence ID" value="BAD39938"/>
        <property type="gene ID" value="STH953"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH953"/>
    <dbReference type="eggNOG" id="COG1447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152490_1_1_9"/>
    <dbReference type="OMA" id="AIFINID"/>
    <dbReference type="OrthoDB" id="2038379at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003188">
        <property type="entry name" value="PTS_IIA_lac/cel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036542">
        <property type="entry name" value="PTS_IIA_lac/cel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34382">
        <property type="entry name" value="PTHR34382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02255">
        <property type="entry name" value="PTS_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000699">
        <property type="entry name" value="PTS_IILac_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46973">
        <property type="entry name" value="SSF46973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51095">
        <property type="entry name" value="PTS_EIIA_TYPE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>ligand shared between all trimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphohistidine; by HPr" evidence="6">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000699-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000699-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="12870" checksum="83FE415D45D6A10F" modified="2004-10-11" version="1">MENLEQLNLIAMRIILDAGDARDFVTKAFEAMGQRDFESADEHLRAAFEKLKSAHRQQTEVIQHEVAAGSLPPSLLFNHAQDTLMVVGSELNLARSLLAIFINIDRRLAQLEAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R13</accession>
    <name>Q67R13_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH895"/>
    <dbReference type="EnsemblBacteria" id="BAD39880">
        <property type="protein sequence ID" value="BAD39880"/>
        <property type="gene ID" value="STH895"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH895"/>
    <dbReference type="eggNOG" id="COG3086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1916022_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="132" mass="13137" checksum="144A7FB5AC8266A7" modified="2004-10-11" version="1">MHIAVNPCSACSHRCGAHSSGGGRYLLVESSTPLKPGRAIVVEAPLPSPARAVALAYLLPLAGFMAGLFIGNAAFDGAPLPVLGSGLAGAAAAYAGVAWTERSRRARVAGATEAEPEGACMPGTAALAPEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R25</accession>
    <name>Q67R25_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PGA_cap domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH883</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CapA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195015.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH883"/>
    <dbReference type="EnsemblBacteria" id="BAD39868">
        <property type="protein sequence ID" value="BAD39868"/>
        <property type="gene ID" value="STH883"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH883"/>
    <dbReference type="eggNOG" id="COG2843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038823_0_0_9"/>
    <dbReference type="OMA" id="WGTEWQD"/>
    <dbReference type="OrthoDB" id="1003186at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07381">
        <property type="entry name" value="MPP_CapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019079">
        <property type="entry name" value="Capsule_synth_CapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09587">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00854">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269627" description="PGA_cap domain-containing protein" evidence="4">
        <location>
            <begin position="39"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="PGA_cap" evidence="5">
        <location>
            <begin position="82"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="445" mass="48493" checksum="1B44463A0827DA5A" modified="2004-10-11" version="1">MKTRRRGATWVAPAVALLACLLLLSGCTGRGPGGGAGAPQNAGGEAQAPGPSGGAEEPAGPETGPGSSEDADPEQEERRTARILAVGDLLMHLPLVYASALPDGEWDFTPLFEPVRPWIEAADLAVANLETTLTGPDYPWAGYPSFNTPPEFARDLKAVGFDVLTHANNHSLDYLKFGIEKTHEALERYGMPHTGTARTPDEREEILVVEPVPGIRVAVLAYTATTNWIPLPEPWSVNLVDDEQMPADIRRARAREDVDLVIVALHFGEEYAREPNDEQRHYVDVALQAGADIVLGSHPHVIQPIEVRQVRDEFGRDLPRAVVYSLGNFISNQEGLYREAGIMMLIDVAKEGGVTRIEQVSFVPTWVHGYRAEGRQRYRVVVVEQSLRDCEAGLDPLLTPADCQRLREVWADTTRQAAGSPDVAVWSVDLPPQHPRAQAVAPGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA0</accession>
    <name>Q67RA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl-carrier-protein) synthase III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194941.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH808"/>
    <dbReference type="EnsemblBacteria" id="BAD39793">
        <property type="protein sequence ID" value="BAD39793"/>
        <property type="gene ID" value="STH808"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH808"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_2_1_9"/>
    <dbReference type="OMA" id="GEEHKEY"/>
    <dbReference type="OrthoDB" id="872193at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="2">
        <location>
            <begin position="112"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="1">
        <location>
            <begin position="248"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="350" mass="38026" checksum="2278364C40CFA5E9" modified="2004-10-11" version="1">MTTTQPHVGVVSTGIYIPDRYMTAEEIADRSGVPLDVVKQKMGILRKPIPGPDDGTAAMGIRAAQQCIERAGIDPAAIDLVIYIGEEYKEYQLWTAGIKTAHAVGAHNAWAFDAQLRCGTWVLGMKLAQALMLSDDRVNTLLLAGGYRNVDYINYANPRVSFMYNLGAGGGAMLLRKNYGRNRVVGSEIIVDGSLSETVGVRGGGTLAGLHNHDDSLYQLDVFDVEYMKTRLNAVSTQRWVEVVSRVCAQGGYRTQDIDYLAILHMKRSAHQALIEQLGIPVDRAIYLEEYGHIGQIDQILSIHLALERGLIRDGDLVVGVSAGVGYAWGATAIEWGPVGATRARKQAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB7</accession>
    <name>Q67RB7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH791"/>
    <dbReference type="EnsemblBacteria" id="BAD39776">
        <property type="protein sequence ID" value="BAD39776"/>
        <property type="gene ID" value="STH791"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH791"/>
    <dbReference type="eggNOG" id="ENOG50337P6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136265_0_0_9"/>
    <dbReference type="OMA" id="AVCAYKA"/>
    <dbReference type="OrthoDB" id="1422646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="16759" checksum="C06E0435BF10327C" modified="2004-10-11" version="1">MAGSVAGRIGGRWAGNDLRVVYADGELTGRMGGKWVGKDVALSFDGAEPTGRWGGRYDGQDLRFSIRGTEGTGRMGGDVVGWDIALVQDGDRITGRIGGAVVGADVRLAARDGLLTGRIGGNFDGKNCTLQVDGVPYLVAAALAAMVFYQLYLDAASTAAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC1</accession>
    <name>Q67RC1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BNR domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH787"/>
    <dbReference type="EnsemblBacteria" id="BAD39772">
        <property type="protein sequence ID" value="BAD39772"/>
        <property type="gene ID" value="STH787"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH787"/>
    <dbReference type="eggNOG" id="COG4447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052334_0_0_9"/>
    <dbReference type="OMA" id="CDKPNTI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028203">
        <property type="entry name" value="PSII_CF48-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14870">
        <property type="entry name" value="PSII_BNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSII_BNR" evidence="1">
        <location>
            <begin position="26"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="344" mass="36790" checksum="64ED6FEA89521313" modified="2004-10-11" version="1">MAVIVAVLASACEAGDRGAPVPSVPTAAAFLDERHGWIGAADGIWYTDDGGRKWTHQFPSQEAVIRLGFVDPQNGWALTQSSAALRTQNGGADWITLDVPGASLRAVDLVSPAHAVATDGNGLLVSRDGGKTWDRSRPPLPLADLDFVSEEEGWAAGEGQVWHTRDGGETWSAQLTLPEPDRWLGDTFVRFPSETSGWVLFCLGQGAAYQETYLLYGTTDGGRTWTPRLVGGWPWPFEAEAPEAPQGPGGYPVAFDARADTAWVAVYSPAAGHLEVVRVTLGGAPPVSSDKIPIEDPRKPTVGISFVDTETGWLVMSDSHQKKTAILRSDDGGTSWTSTIDNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD7</accession>
    <name>Q67RD7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Ribokinase</fullName>
            <shortName evidence="10">RK</shortName>
            <ecNumber evidence="3 10">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rbsK</name>
        <name evidence="13" type="ordered locus">STH771</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="3 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH771"/>
    <dbReference type="EnsemblBacteria" id="BAD39756">
        <property type="protein sequence ID" value="BAD39756"/>
        <property type="gene ID" value="STH771"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH771"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_2_0_9"/>
    <dbReference type="OMA" id="AMDSIPF"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="D_ribokin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02152">
        <property type="entry name" value="D_ribokin_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="12">
        <location>
            <begin position="5"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="223"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="31877" checksum="1E9CDCDC7D91AF45" modified="2004-10-11" version="1">MDRPVVVVGSLNLDLVVNPERAPAAGETVFAPRLDQFPGGKGANQAVAAARLSAPVAMVGRVGQDAFGDRLLESLRADGVATEGISRSATAGTGTAVITVEATGQNRIVVVSGANAEVTPELVEAHGPLIASASALLLQLEVPVEACVRAAELAAAAGVPVILDPAPAPAEPLPEPLRRNTWLITPNETEAAALTGVPVAGRCGAEEAARVLWAQGFRQVLIKLGSEGAYLFRGGAGQWFEPFRVPVVDTTAAGDAFAGGLAAALHRGMALEEAVRWGMAAGALAVTRPGAQPAMGNLDELMTLLRTGRLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI5</accession>
    <name>Q67RI5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH723</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH723"/>
    <dbReference type="EnsemblBacteria" id="BAD39708">
        <property type="protein sequence ID" value="BAD39708"/>
        <property type="gene ID" value="STH723"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH723"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="WSIEHSV"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269508" evidence="3">
        <location>
            <begin position="26"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="218"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="318"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="632" mass="66526" checksum="E342A56D6C3D9F8E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI6</accession>
    <name>Q67RI6_SYMTH</name>
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        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="ordered locus">STH722</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194855.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH722"/>
    <dbReference type="EnsemblBacteria" id="BAD39707">
        <property type="protein sequence ID" value="BAD39707"/>
        <property type="gene ID" value="STH722"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH722"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_9"/>
    <dbReference type="OMA" id="WHKLIEQ"/>
    <dbReference type="OrthoDB" id="384611at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:cellular aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
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        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="8">
        <location>
            <begin position="7"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 6">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="477" mass="51844" checksum="E035015CDFDF451D" modified="2004-10-11" version="1">MYIGGEWREAVSGRRFEVTNPATGEVIATVPDAGREDARAAIEAAHAAFGEWSSLPAHVRARYMRRLYDLIVQNVDRLARVLTEENGKPLAEAKGEVFNGAEYLNWYAEETRRIYGETIPSSSRRTRIWVLRQPVGVVGAITPWNFPSSMVTRKIAPALAAGCTVVLKPAEQTPLSALLIAELIHEAGLPAGVVNVVTTLDPAAVGQEFLENRLVRKISFTGSTEVGKHLMQGAAAQMKRVSMELGGNAPFIVFEDADLDAAVQGVVISKFRNAGQTCICANRIYVQESIAPAFTERLVEAVRGLRVGHGLEEGINVGPLIDEAAYVKVEGHVSDARGKGARVLTGGHRLTGPGFDRGFFYAPTLLDQVSDEMRIVHEETFGPVAPILTFRTEEEVIRRANDTNYGLAAYIFTRNLGRTVRVSEALEYGMVAVNDSLLAVPQAPFGGVKESGLGREGGHHGLDDYLEYKYLSVVLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ5</accession>
    <name>Q67RJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Anaerobic dimethyl sulfoxide reductase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dmsA2</name>
        <name evidence="9" type="ordered locus">STH713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194846.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH713"/>
    <dbReference type="EnsemblBacteria" id="BAD39698">
        <property type="protein sequence ID" value="BAD39698"/>
        <property type="gene ID" value="STH713"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH713"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_9"/>
    <dbReference type="OMA" id="HIWEKTR"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="8">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="858" mass="95585" checksum="2A6C3FA80C933F02" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM3</accession>
    <name>Q67RM3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194818.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH685"/>
    <dbReference type="EnsemblBacteria" id="BAD39670">
        <property type="protein sequence ID" value="BAD39670"/>
        <property type="gene ID" value="STH685"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH685"/>
    <dbReference type="eggNOG" id="ENOG5033F5A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1481317_0_0_9"/>
    <dbReference type="OMA" id="WHGRSAF"/>
    <dbReference type="OrthoDB" id="2142034at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="19543" checksum="687B377A9FA0697E" modified="2004-10-11" version="1">MRHEQIIVAAVAGVFFVWYFAGAWLNRRRGAQILGAVRQAVGAVGRGATVRWHGRSAFEVAVAEPLAPLAAFRLLCLLEPRDFPLALAWNRLRGRRDQVVIHAEFARPPREGRPLALAECDIPGLTGAALSGSPPHLRLTLQVAVGGEDAIARAFALARQLAEQPPRGQPPGGVRAASAGGAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM7</accession>
    <name>Q67RM7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arsenic transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194814.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH681"/>
    <dbReference type="EnsemblBacteria" id="BAD39666">
        <property type="protein sequence ID" value="BAD39666"/>
        <property type="gene ID" value="STH681"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH681"/>
    <dbReference type="eggNOG" id="COG0003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_4_0_9"/>
    <dbReference type="OMA" id="FGYKLDA"/>
    <dbReference type="OrthoDB" id="1225869at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="Anion-transp_ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="PTHR10803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA_ATPase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="339" mass="38124" checksum="595CC9F5EF00C7E5" modified="2004-10-11" version="1">MPKGLGAYFEQNPDAEIVIFAGKGGLGKTTSSSGLAYYMSQVKKKRTLLFSTDPQASLSDIFERNIYGQGEVEILPNLFVVEIDADRRVAEYQQQVKQKIMDMYGLDAVPREIEEYIDSTSAEPAMYESATYDAMAELVARKEYDIYIFDMPPFGHGVRMVAMADILSKWVEKITDARAKVAEYDAVAATLKGEAAREDEVMKELIDIRNKIKSFTDLLTARRRTAFFMVLIPEQMAILDTERALTMFENLGIEMSGLVVNQVYPAELLQQDGTSDYLRNRVAMQQEHLRTIAQKFGDKVQAVVPMFTREPKGIEMIKTASEYLINCQVELPAVRGGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR4</accession>
    <name>Q67RR4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sigma-54-dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH644</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194777.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH644"/>
    <dbReference type="EnsemblBacteria" id="BAD39629">
        <property type="protein sequence ID" value="BAD39629"/>
        <property type="gene ID" value="STH644"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH644"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_6_9"/>
    <dbReference type="OMA" id="DWTGNIR"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="6">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="5">
        <location>
            <begin position="151"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="470" mass="51041" checksum="A466F9EE5EEDAE17" modified="2004-10-11" version="1">MENGVLQASLGPLLDLVDLAVTIVDAEGRVAAWNEGAERLYGIAAPDILGRAITGFFTPSSLMVRRVLESGQRVEWTYHQPRPGIYVLVSAAPVIRDGQVVGAIAVERDVTDLVELSNELLQARGQVDALQERLSPSPAPAPPSADPFQAVRGRHPAIRRAIRLASLVAATDATVLIRGETGAGKELFARAIHQASRRRDGPFVVLNCGAIPATLFESELFGYAPGAFTGANPKGQRGKLELAHGGTLFLDEVGDLPLESQVKLLRFLEDRKFYRVGGSTPITVDVRVVVATNRDLEELVRQGRYRDDLYWRLNVFTLDIPPLRERREDIAELMQRFLHEFSMRYGRPPAEVHPAVIQAMMEHTWPGNVRELRSAVERMVILSPDGAVGPELIPAILGPARRGAAAAGPAAPPCAPGAAPQDEREAIVAALAATRYNRSAAARLLGISRGTLYNRMRRLGLDAQTAQGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY2</accession>
    <name>Q67RY2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GyrI-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH576"/>
    <dbReference type="EnsemblBacteria" id="BAD39561">
        <property type="protein sequence ID" value="BAD39561"/>
        <property type="gene ID" value="STH576"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH576"/>
    <dbReference type="eggNOG" id="COG4832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083625_0_0_9"/>
    <dbReference type="OMA" id="GKHHEIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029442">
        <property type="entry name" value="GyrI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008319">
        <property type="entry name" value="UCP031644"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06445">
        <property type="entry name" value="GyrI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031644">
        <property type="entry name" value="UCP031644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="SSF55136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GyrI-like" evidence="1">
        <location>
            <begin position="31"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="23884" checksum="3BB6B2D04526113D" modified="2004-10-11" version="1">MEPEPHHTRKGASVMTQPAGFAPVLFKARKDPEIVEVPDTWCLTLSGVGAPESPAFQQAVEALYGLAYTLKFQQKAAGQDFKVPPLEGLWWSEPPEAFARTPREEWRWQLLIRMPEGITAEQVAATRGQAAAKKKNPRILDVAFERFAGGTAAQVLHVGPFSEEGPVIEQLHAFIAAQGYRLRGRHHEVYLSDFRRTPPEKLKTILRQPVEPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S34</accession>
    <name>Q67S34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194658.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH524"/>
    <dbReference type="EnsemblBacteria" id="BAD39509">
        <property type="protein sequence ID" value="BAD39509"/>
        <property type="gene ID" value="STH524"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH524"/>
    <dbReference type="eggNOG" id="COG1307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048251_3_2_9"/>
    <dbReference type="OMA" id="GRCSGMQ"/>
    <dbReference type="OrthoDB" id="1288126at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="285" mass="30877" checksum="56703285D28EC0C3" modified="2004-10-11" version="1">MPSIRIMTDSTADLSPELLQAHGVRVVPLLVQFGEETYHDGVDMDTGRLFQMVTERNQLPKTASPGPGLFRSHFEEATADGSQVLYISISAKFSAALQNARIAAADFPGQVHVFDSRNLSTGIGLQVLHACDLVRQGKSVEEILADLETYQAGVRSSFVVDTMEYLHKGGRCSGVAALVGTLLKIRPVISVVDGGMIVAAKVRGPRKKALDWMLERFAEDAAQGRVRPERVFVTSPDSPHEDAPYMVAEIRRILPEVQEVLVTRAGAVIGSHCGPGTIGILYATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S75</accession>
    <name>Q67S75_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH483</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194617.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH483"/>
    <dbReference type="EnsemblBacteria" id="BAD39468">
        <property type="protein sequence ID" value="BAD39468"/>
        <property type="gene ID" value="STH483"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH483"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027579_1_1_9"/>
    <dbReference type="OMA" id="HIPWCVR"/>
    <dbReference type="OrthoDB" id="1130951at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010723">
        <property type="entry name" value="HemN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06969">
        <property type="entry name" value="HemN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="416" mass="45893" checksum="4AA74F6970133ABA" modified="2004-10-11" version="1">MPIGLYVHIPFCLHKCGYCDFNSHAGSDRAEQAHYVDALLREMELWATRPEVAGEQVSTVYVGGGTPTLLEGAELARVLRGVREHFRLAPDAEVTVEGNPGTVDVEGEKLHLAVQAGATRLSLGVQARQRHLLERLGRIHDALQVEAAVKAARRAGFANLNLDLMYGLPGQTPADFRETVSWALGLGPTHISAYSLIIEEGTPFYHAYLAGRLHLPPEEAEEQMFLEGKALLEAAGFEHYEVSNWARPGFRCRHNLIYWRNEHYLGLGCGAHSFLRLAAPLSNLGPPRPGLTASRVQGGSPVETAPGLPGQQYRFWNLKHPGAYRAALERGVLPVEAGEVVDRRAEMAETMFMGLRLLEGVSGERFEARFGVSIADVYGPDVDRLRREGLLEWAEGALRLTPRGLRLGNRVWEAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S78</accession>
    <name>Q67S78_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 9">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">greA</name>
        <name evidence="12" type="synonym">greA1</name>
        <name evidence="12" type="ordered locus">STH480</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the GreA/GreB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194614.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH480"/>
    <dbReference type="EnsemblBacteria" id="BAD39465">
        <property type="protein sequence ID" value="BAD39465"/>
        <property type="gene ID" value="STH480"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH480"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_2_1_9"/>
    <dbReference type="OMA" id="ILQEQME"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="11">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="10">
        <location>
            <begin position="87"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="14"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17859" checksum="0F9AB7B6B6231991" modified="2004-10-11" version="1">MEENKPVVLSPEGLQQLQEELDYLRNVKRKEVAERLKEARSYGDLSENSEYDDARNEQAFVEGRIAMLENTLRNAVIMDESEDEAEAGKIRLGSTVVLKDLEYGDLLEYTIVGTVEADPAKNKISNESPVGKAIMGRTKGEVVVVEAPAGRIEYEIVDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S98</accession>
    <name>Q67S98_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RDD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194594.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH460"/>
    <dbReference type="EnsemblBacteria" id="BAD39445">
        <property type="protein sequence ID" value="BAD39445"/>
        <property type="gene ID" value="STH460"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH460"/>
    <dbReference type="eggNOG" id="COG1714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054176_1_0_9"/>
    <dbReference type="OMA" id="WMLTGGP"/>
    <dbReference type="OrthoDB" id="1974702at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="19"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="27840" checksum="E56FD9EB42DC6D05" modified="2004-10-11" version="1">MDPIRIDTPESVDLALEPVGLGSRFLAALIDAAIQYAVLFAVLLVALPSAALWTDLGEGFDLIGSAFLAILLLAVALLFFLYKMLFEALWNGQTIGKRVAGIRVVQVSGMPVTFLQAVIRNLLRIVDYLPGYYLIGAICILATGRRQRLGDLAAGTVVVRDRPAEAPEVPKALSHPPQTDLNKLREYALRLREEDLAPARGFWQRRHQLHVSARSRVAEQVARGLAARMGWPDPLPPVPEDFIEEVLYVRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA5</accession>
    <name>Q67SA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH453</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194587.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH453"/>
    <dbReference type="EnsemblBacteria" id="BAD39438">
        <property type="protein sequence ID" value="BAD39438"/>
        <property type="gene ID" value="STH453"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH453"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_9_9"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="22"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="337"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="370"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="579" mass="63200" checksum="4CFF92E273A985D2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB0</accession>
    <name>Q67SB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194582.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH448"/>
    <dbReference type="EnsemblBacteria" id="BAD39433">
        <property type="protein sequence ID" value="BAD39433"/>
        <property type="gene ID" value="STH448"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH448"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1098065_0_0_9"/>
    <dbReference type="OMA" id="EQDEYEN"/>
    <dbReference type="OrthoDB" id="670529at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="128"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="192"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27403" checksum="FEBF1287F234D63B" modified="2004-10-11" version="1">MRSRDITLTGTYLPNTPVTLVVDGVAQVQGMTDSAGRFRLTGTLEPGRNRVYVMGEGPLASREYAVRYLPPYTDMAGHWAEAVVDALHERGVVSLYPLPRFEPEAQVTRMEFAVMVARALRLPAAEEQAPFTDVAVMPQWALLEVAAAVKAGIIKGMPDGSFAPDRLVTRAEMAAMLTRALAWAGLEVKPEAPEFADQAEIPDWAVEPVAAAVRHGLIKGYPDGTFRPGSPTTRAEAATMVHRLLQTVIGLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-07-11" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB6</accession>
    <name>Q67SB6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tnaT</name>
        <name evidence="9" type="ordered locus">STH442</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194576.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH442"/>
    <dbReference type="EnsemblBacteria" id="BAD39427">
        <property type="protein sequence ID" value="BAD39427"/>
        <property type="gene ID" value="STH442"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH442"/>
    <dbReference type="eggNOG" id="COG0733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_3_3_9"/>
    <dbReference type="OMA" id="GMATFIF"/>
    <dbReference type="OrthoDB" id="376209at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="PTHR11616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SSF161070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="501" mass="54750" checksum="EEF0703FE26CF5DD" modified="2012-07-11" version="1">MEAQRDQWKSRSGFIFATIGAAVGLGNFWRFPFMAYQNGGGAFLLPYFVALLTAGVPLMILEFGFGHKMRTATITAFKKLNRRFEWIGWWQITVPVVVVTFYSVIISWSLRYLIFSFTQAWGDDPGTFFSSDFLGITSGPLELGGLRWGIFAAVAVVWFANYYISAQGISGGIEKACKIMTPFLIVAMLIFVIRGITLPGATYGLNYFLNPDFSKIMDPGVWVAAYSQVFFSTTLAVGVMIAYASYVPEDSDLANNAFITVFANSSFDFMAGLAVFSTLGYAAVTAGVPFEEMAVAGPGVAFVAFPKAISMLPGPTWLQSLFGILFFSALLLAGISSSISQMESFASAVIDRFGVDRKKLLGWFSLIGFAFSALFATGAGVHILDIVDHFVGSYAIAILGLVEAIVLGYIMGTARIREHVNLTSDIRVGMWWDVLVKYVTPVLLGYNILSNFISEFREPYAGYPTGALVLFGWVVAIAMFGTSLFMQWRSQQLDVTGGEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF1</accession>
    <name>Q67SF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH407</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194541.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH407"/>
    <dbReference type="EnsemblBacteria" id="BAD39392">
        <property type="protein sequence ID" value="BAD39392"/>
        <property type="gene ID" value="STH407"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH407"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_911925_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32788" checksum="629F06A688A93561" modified="2004-10-11" version="1">MIISPAETQALEPAALALLEIYRKGEHTALLAEVDQQSKSGPLPPDVLGLAAASLLALERYDEAVKAAGHATAQSPHKAWLHHVLGRAELGRGDRDKALQAAQTACRLMPDYPDYLATLAACQRESGDPAAAAATARRALAAAPDHAAALLELGLALAALGDEAGALERLREAQAAEPKAAAPYLAEADLHTKAGRTAEARRALQRALRELPDLVEAEERLAGTVGRSALLHRTLIHLIHLSRLTVTGWLIVAFLYYLFFRLLEFLWKYFAVLLPVGRVLLAFTALWLLGGAVAGRLLRGTLRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS4</accession>
    <name>Q67SS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH284</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH284"/>
    <dbReference type="EnsemblBacteria" id="BAD39269">
        <property type="protein sequence ID" value="BAD39269"/>
        <property type="gene ID" value="STH284"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH284"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="285"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="541" mass="60929" checksum="23749F5D6AC3B375" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST4</accession>
    <name>Q67ST4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS21 family transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194409.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH274"/>
    <dbReference type="EnsemblBacteria" id="BAD39259">
        <property type="protein sequence ID" value="BAD39259"/>
        <property type="gene ID" value="STH274"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH274"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_1_1_9"/>
    <dbReference type="OMA" id="AYEKRSV"/>
    <dbReference type="OrthoDB" id="1792718at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="99"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="29912" checksum="8F80E2508A3959CB" modified="2004-10-11" version="1">MSQELLLDYYLKRLKLPTVRRLYKDLARDAAEHNKTFEEYLQALMEQEVIHREDNQLQRRLKAAGFPVPKTLESFDFTVIPSVNKAKILALSKSEYIRDRENVLFVGNSGTGKTHLATALGIAACRQGYRVRFWRVGQLVAELQEAQNEHRLSRLEKQWLRLDLVVLDELGYVSLSRTASELLFHFVAARYERGSLMITTNLEFSEWPQVFGDEKMTAALADRVTHKAHIIEMNGESYRFRETLRRQEALKQRLESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU6</accession>
    <name>Q67SU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194397.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39247">
        <property type="protein sequence ID" value="BAD39247"/>
        <property type="gene ID" value="STH262"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH262"/>
    <dbReference type="HOGENOM" id="CLU_1057391_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28260" checksum="5EC7A0C0323F680D" modified="2004-10-11" version="1">MAAILYRHGLAVVKAGGLAEINLLAVAGERLVGAVEVKRRYHEAARYPTLYINLRKVAAMATWSVALGVPAWFVVVYDDQVQAISLADLPGAVGGCRVDGYRVRHGATALEPVLEVRVNAMRLIGRTEETAEVEIGGPASIPDQSRMVDLDGQPLLRDQATLDQAVAIAGRLAPLLGGDRVGHFGPISPLDCWAARGDRPLVLVLTTCLPLPPEWHRVVTLPLRLVLALQATAQLAGLRAVVAVQADTDLCWLERTSRSHRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ5</accession>
    <name>Q67SZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194348.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH213"/>
    <dbReference type="EnsemblBacteria" id="BAD39198">
        <property type="protein sequence ID" value="BAD39198"/>
        <property type="gene ID" value="STH213"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH213"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_9"/>
    <dbReference type="OMA" id="QYVAHVE"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="27545" checksum="1722A449F70C27B2" modified="2004-10-11" version="1">MRCALDGEGVFRITLNRPERLNAFNQAQHEGLIAALQQAERDPAVRCVVITGEGRAFCAGQDLQSIGDMPIGDLVRRYYNRWIAQIRSLEKPVLAAVNGVAAGAGVSLALACDLIVMAESAAFIQAFARIGLVPDSGASYFLPRLVGYHKAMELALFGDRVSAAEALSLGLCNRVVPDDQFEPAVAEWAGWLARGPRAVGWIKRQLNRGLERSLDEVLALEAYHQEIAAATEDAREGIRAFVERREPRFTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T27</accession>
    <name>Q67T27_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH181"/>
    <dbReference type="EnsemblBacteria" id="BAD39166">
        <property type="protein sequence ID" value="BAD39166"/>
        <property type="gene ID" value="STH181"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH181"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="YKHQEIA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="4">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="5">
        <location>
            <begin position="128"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="23882" checksum="010FF1137218030A" modified="2004-10-11" version="1">MSPRDETLAFAAQQGDMDAFAALVERHKGRVYRTLYQVVGDAADAQDLAQEVFVKVFRNLGSYRGDAAFTTWLHRVTLNVAFDWLRARKRRPLTVPLEPPPGEEEHRQADLPSPEAGPEELTLREERRRRLRDAIDRLSPDYKEVVLLYHFYHLSYQQIADRLGVPVRTVETRLYRARANLKRMLVSWEGGERVEVQRCAPAPGSVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T35</accession>
    <name>Q67T35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194308.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH173"/>
    <dbReference type="EnsemblBacteria" id="BAD39158">
        <property type="protein sequence ID" value="BAD39158"/>
        <property type="gene ID" value="STH173"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH173"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_665531_0_0_9"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="32"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="42057" checksum="2997D21A1C422A02" modified="2004-10-11" version="1">MHATLAAAIWAVLLLGGAFRPGAATPDGPPRPAVGSPAPALELADPTGQTVRLADFRGRAVFLNFWASWCGPCRAEMPEIQRLAESLPDGTAVLTVNLTGREASPSAPLAYLAEHGYSFPVLLDPRDEAGTAFQVASMPTSLFISPEGAVTARVAGPLTYRAMREYLTAAVSGPPSATAGRSLNRWLPESVPLGPAALPTRALLWLAGVLLAAAVAGVPRPVTDARRSAAEAESDPGPSLRARELVLNLAVGGVLGAKLLYVLMDPGAYVRSPALLLAFPYGRLALPGAVAGGLALSLWGLRGVPDSATVWDRALPGLLLGAGVGALASPDPADWALGPALLAAGLLSLGLSRLAGPPGDGAVTALLLGALAVTLADLARPSAGPAGITPVQLLAAVVATLAWIRQRRRTVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T42</accession>
    <name>Q67T42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH166"/>
    <dbReference type="EnsemblBacteria" id="BAD39151">
        <property type="protein sequence ID" value="BAD39151"/>
        <property type="gene ID" value="STH166"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH166"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1401832_0_0_9"/>
    <dbReference type="OMA" id="MANCICA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="2">
        <location>
            <begin position="77"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="22273" checksum="962FCF196C97A0E1" modified="2004-10-11" version="1">MRYPRRGQTEQKGANRQVPRGGMNTTDPNLIEVIDSRGRTLTPCRRKVADREVRLGRAKWIGRRRIRLRFDPFAYRHIRLKVLARDRYTCYWCGRPGFTMDHVIPWSKGGRTTMANCICACEECNGRRGDMDAAEFAHLMGRPTPHPQLILSGRHLAAEAAAAPEPVTGTVRPHRFARLARLLEAEPVRRRSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T46</accession>
    <name>Q67T46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194297.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH162"/>
    <dbReference type="ESTHER" id="symth-q67t46">
        <property type="family name" value="Bacterial_EstLip_FamX"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39147">
        <property type="protein sequence ID" value="BAD39147"/>
        <property type="gene ID" value="STH162"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH162"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033707_0_1_9"/>
    <dbReference type="OMA" id="YNKRTYE"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269362" evidence="2">
        <location>
            <begin position="27"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="3">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="4">
        <location>
            <begin position="283"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="536" mass="57772" checksum="F3623C5E725E92CD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T79</accession>
    <name>Q67T79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194264.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH129"/>
    <dbReference type="EnsemblBacteria" id="BAD39114">
        <property type="protein sequence ID" value="BAD39114"/>
        <property type="gene ID" value="STH129"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH129"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_1_9"/>
    <dbReference type="OMA" id="AIQLPWF"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="33197" checksum="9E19030531F65653" modified="2004-10-11" version="1">MLAYIVRRLWSGIPVLLGVSLITFILMHVVPGDPVTVAFEKKADPATIERIRREMGLDRPLPVQYADFLLRALRGDLGRSFQTQQPVMNMIRSAMPATLKLTASAVLVSVVIGVPIGILSALNRGTAIDYMATVTALSFISAPVFWVAMVAQLLFGYRLGWLPISGFDTLKHLILPALVLGTRYAASIARYTRSSMLDVVSQDYIRTARAKGLSGRVVVFKHALKNALIPVITVVGLEIGGLLTGSILTESVFGIPGLGLVTIRGLNALDFPVIQATVLLTAVIFVVMNILVDISYSLVDPRIRMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T81</accession>
    <name>Q67T81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sodium-dependent transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH127</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH127"/>
    <dbReference type="EnsemblBacteria" id="BAD39112">
        <property type="protein sequence ID" value="BAD39112"/>
        <property type="gene ID" value="STH127"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH127"/>
    <dbReference type="eggNOG" id="COG0471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_0_0_9"/>
    <dbReference type="OMA" id="LHGMNTY"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="495" mass="52088" checksum="BBD83F1DAE2B6156" modified="2004-10-11" version="1">MQGARATVAGEKQLSLNLAQRIGLVLGPTLCLLIYLFFRPAGLGPEGVAALAGTAWIAAWWVTEAIPIPITSLLPVILFPVLGLGSVGEVVSAYADPTLFLFLGGFIIAVAIERWNLHRRIALAVISAVGTSPSRLVLGFMLATGVLSAFISNSATAMMMLPIGIAVVKQITELRVRNGEKVYPDQEPFSALILLAIAYAASIGGVGSIIGSPPNAIMVGVAASTLGVEISFFDYMLVGVPMFLVFTLVAWRILLWVMPPGFAEVAGSREWVRRQMAELGPMSGEERKVLGVFCATALLWITYPFLLKGVVPGISDAMVAIFGASLLFLIPGGEGGFLLDESALKELPWGVLLLFGAGFCIAEVFQSTGVAVWLAGFLQVLAGAPYFLIVIAVAAMVTFLTEITSNTAIAAIFMPIMASLATALGAAPTGLMVVAALAGSLAFMLPVGTPPNAIVFSSGRVTIRQMIRVGFFLNLAAIATITLIGCFWVPLVWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T84</accession>
    <name>Q67T84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194259.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH124"/>
    <dbReference type="EnsemblBacteria" id="BAD39109">
        <property type="protein sequence ID" value="BAD39109"/>
        <property type="gene ID" value="STH124"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH124"/>
    <dbReference type="eggNOG" id="COG1920">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_745819_0_0_9"/>
    <dbReference type="OMA" id="PPENDNI"/>
    <dbReference type="OrthoDB" id="1654117at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="371" mass="40283" checksum="A269A4EC9894C89C" modified="2004-10-11" version="1">MRTAAIIFEGGPRAENPLQETLTGLRHAVTLDTVAKFCAAGLDQVVLATNHPALAEAAARLGARIFDTRGDRFHFGRSLVQAVRESGADAVIYLSGAALPLIGQEEIAWIRDALRRDEPTVVVNNVQSVDLVAWRPASRLERVDPPENDNILGWRLRDTGMERVLLPNSAAVNFDLDTPTDYLILALSGKGGPRAQAALRAAGWSDARLRAAADVLAAELPEVALIGRVGSAIMEHFNRNLPVRLRVFSEERGMKALGREAAGLVRSVVGDLIEDLGPERFFARLGETCDAAFFDTRVLFANRGRRVSEWDRFQSDLGNVEQIGDPFVRAFTRAALECPIPVVLGGHSVVAGGLWVLADRAIALRGGPMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA7</accession>
    <name>Q67TA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stage II sporulation protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH101"/>
    <dbReference type="EnsemblBacteria" id="BAD39086">
        <property type="protein sequence ID" value="BAD39086"/>
        <property type="gene ID" value="STH101"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH101"/>
    <dbReference type="eggNOG" id="COG2385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021203_1_1_9"/>
    <dbReference type="OMA" id="LCQWGAK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013486">
        <property type="entry name" value="SpoIID/LytB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013693">
        <property type="entry name" value="SpoIID/LytB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014225">
        <property type="entry name" value="Spore_II_D_firmicutes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30032:SF4">
        <property type="entry name" value="PTHR30032:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08486">
        <property type="entry name" value="SpoIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02669">
        <property type="entry name" value="SpoIID_LytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02870">
        <property type="entry name" value="spore_II_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269974" evidence="1">
        <location>
            <begin position="25"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="SpoIID" evidence="2">
        <location>
            <begin position="72"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="361" mass="39159" checksum="9B206BC96DCD0FD1" modified="2004-10-11" version="1">MLRLLLAAVLMTAALTLALPAGIGAGVRMPQPPDPLPVPVLVPWAPEPEAPVDEPAGADSLDVKVYFPDRDAIVVMPLGEYLKGVVAAEMPADFEMEALKAQFVVARTYTVRRMPQFGGQGGCPLNPEADVCADYRTSQAYMSREELEAKYGPLAAASFWRRLSQAQAETEGEVLTYQGELIDALYHAVSGRMTESSGDYFATDLPYLMPVDDTWGAAAPRLVDERRFSPEAFVRALGLGSEEPLLAVQAAARVGQPPVQVTARTAGGRVKTVAIGGMTLTGRQVRERLGLRSTDFRVYWQDGEIVVETFGDGHGVGMSQYGAQGMARAGKTYREILTHYYTGVELSRLFEINGENWVPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB4</accession>
    <name>Q67TB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Conserved hypothetical iron-sulphur protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH94</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194229.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH94"/>
    <dbReference type="EnsemblBacteria" id="BAD39079">
        <property type="protein sequence ID" value="BAD39079"/>
        <property type="gene ID" value="STH94"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH94"/>
    <dbReference type="eggNOG" id="COG1139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_2_0_9"/>
    <dbReference type="OMA" id="HCPVYDK"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019516">
        <property type="term" value="P:lactate oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="4Fe-4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="PTHR47153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00273">
        <property type="entry name" value="TIGR00273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="358"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="486" mass="53486" checksum="62E941DF1784A8FF" modified="2004-10-11" version="1">MAGKAVLRQRVARALADDHLRGAVRYTSENLYLKRKAAMADLEALAARGEAIGTFAELRARAQEIKGHTLAFLDGYLKQAADQIRKSGGVVHWARDAAEANQIVLEICHRRGARKLIKSKSMVTEEIRLNHALEAEGLEIVESDLGEYIIQLARETPAHIVIPAIHKTRRQIADLFGAVAGRELATDTRTLAGFARETLRHKFMTADVGVTGANFVVAETGTICLVTNEGNGRLTTSVPPCQITLVGIEKLVPNLEDLAVLMALLPRSATGQKMTTYFNLITGPRRPGEPDGPEELHVIFLDNGRSEILATRYREVLACIRCGACLNACPVYRQLGGHAYGSVYPGPIGTVLTPLLQGLDPWDELLQYCSLCGACTENCPLGIQLHKHIIHLRGEAVARGREHPLMRTALKAVHFAWTRPGVYRLVARGAHLATRPFARRRPDGSAYLDRLPSIAAGWTFSRTLPSPARKPFHRRWAELEREEGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC5</accession>
    <name>Q67TC5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH83</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194218.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH83"/>
    <dbReference type="EnsemblBacteria" id="BAD39068">
        <property type="protein sequence ID" value="BAD39068"/>
        <property type="gene ID" value="STH83"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH83"/>
    <dbReference type="HOGENOM" id="CLU_2453520_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032820">
        <property type="entry name" value="ATPase_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09527">
        <property type="entry name" value="ATPase_gene1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="89" mass="9839" checksum="5F35F1CF1AFF8755" modified="2004-10-11" version="1">MHRRSPRRSPYLFAAIDFGYTLLASLGLFGGLGWWLDGKLRTAPLFLIAGILLGLAVAFNGLLRRLNAIDRAVKAAKKEETQKTRDGQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD0</accession>
    <name>Y078_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0340 protein STH78</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH78</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0340 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TD0"/>
    <dbReference type="SMR" id="Q67TD0"/>
    <dbReference type="STRING" id="292459.STH78"/>
    <dbReference type="EnsemblBacteria" id="BAD39063">
        <property type="protein sequence ID" value="BAD39063"/>
        <property type="gene ID" value="STH78"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH78"/>
    <dbReference type="eggNOG" id="COG4475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106658_0_0_9"/>
    <dbReference type="OMA" id="FIGMHLR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00800">
        <property type="entry name" value="UPF0340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028345">
        <property type="entry name" value="Antibiotic_NAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006340">
        <property type="entry name" value="DUF436"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04260">
        <property type="entry name" value="DUF436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007510">
        <property type="entry name" value="UCP007510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110710">
        <property type="entry name" value="SSF110710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01440">
        <property type="entry name" value="TIGR01440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000213029" description="UPF0340 protein STH78">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00800"/>
        </source>
    </evidence>
    <sequence length="177" mass="18505" checksum="5AB1460272F2C4AC" modified="2004-10-11" version="1">MRAEARAAVGELLEAAGLSPGQVLVVGCSTSEVIGRRIGTAGSEAVAGAILEPLLEATQAAGVHLAVQCCEHLNRALVVERAAAERYDWERVTVVPVPRAGGALAARAMRRLPDAVVVEEIKADAGLDIGLTLIGMHLRRVAVPVRLKTAAIGHARVVAARTRPKLIGGARAVYEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI5</accession>
    <name>Q67TI5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH23</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH23"/>
    <dbReference type="EnsemblBacteria" id="BAD39008">
        <property type="protein sequence ID" value="BAD39008"/>
        <property type="gene ID" value="STH23"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH23"/>
    <dbReference type="eggNOG" id="COG3448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040397_3_0_9"/>
    <dbReference type="OMA" id="GAYSWSY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="19168" checksum="2A936030856D26ED" modified="2004-10-11" version="1">MMKKYLALMRGQQLVSSIPYPGLAEALVSGLGGFIVITLLTYVHFEVQAIGLFIVPFGASAVLAFAAPGAPFSQPRNILGGHLVAALTGVVVYMLTGSSAFWAMGLASGLAIALMVLTKTVHPPADATALLPVINQIADPMWAISPVLAGAAVIVIVALLYNNLWARTDKYPRRYPSFWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ5</accession>
    <name>Q67TJ5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase III subunit gamma/tau</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dnaX</name>
        <name evidence="7" type="ordered locus">STH13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194148.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH13"/>
    <dbReference type="EnsemblBacteria" id="BAD38998">
        <property type="protein sequence ID" value="BAD38998"/>
        <property type="gene ID" value="STH13"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH13"/>
    <dbReference type="eggNOG" id="COG2812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006229_0_3_9"/>
    <dbReference type="OMA" id="EIHCKMV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022754">
        <property type="entry name" value="DNA_pol_III_gamma-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12169">
        <property type="entry name" value="DNA_pol3_gamma3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="37"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="367"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="378"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD38998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="570" mass="61602" checksum="48217BB005CC372C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK7</accession>
    <name>DNAA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaA</name>
        <name type="ordered locus">STH1</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194136.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TK7"/>
    <dbReference type="SMR" id="Q67TK7"/>
    <dbReference type="STRING" id="292459.STH1"/>
    <dbReference type="EnsemblBacteria" id="BAD38986">
        <property type="protein sequence ID" value="BAD38986"/>
        <property type="gene ID" value="STH1"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026910_3_1_9"/>
    <dbReference type="OMA" id="REFNPLF"/>
    <dbReference type="OrthoDB" id="219876at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11638">
        <property type="entry name" value="DnaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114282" description="Chromosomal replication initiator protein DnaA">
        <location>
            <begin position="1"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="161"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <sequence length="458" mass="51597" checksum="E3F6990A94B40074" modified="2004-10-11" version="1">MKTELSEVWQQCIDHLERHTSKTAIENWVRSTRPVAVVEDTIIVGVQGEFAKHRMESRYAPLLRQALRDLTKRNMQLKFVANPNVIEDPAAEPVDAPNVADLPAGTSAPAAEQNARLLGYINPKYTFETFVVGNSNRFAHAAALAVAETPARTYNPLFIYGGVGLGKTHLMHAIGHYVLQHNPTAKVAYVSTETFTNEFIMAIQKGSTTAFQNRYRKVDVLLIDDIQFLAGKEATQEEFYHTFNAIREANKQIVISSDRPPKEIPTLEDRLRSRFEWGLICDIQPPDLETRTAILRKKAQSEGIQVPDEVTNYIATNIETNIRELEGALTRVVAYANMLKCPLTYDLAVQALKDILPPVRPKQITIATIKQVVAEHYNIRMQDFEVRNRSRAVAYPRQIAMYLARELTDSSLPKIGEEFGGRDHTTVIHACEKIAKDIQSDPAFAHTIEQLIARIRAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q3V818</accession>
    <name>PDXA2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative D-threonate 4-phosphate dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.408</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH2300</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the NAD-dependent oxidation and subsequent decarboxylation of D-threonate 4-phosphate to produce dihydroxyacetone phosphate (DHAP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-O-phospho-D-threonate + NAD(+) = CO2 + dihydroxyacetone phosphate + NADH</text>
            <dbReference type="Rhea" id="RHEA:52396"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:136590"/>
            <dbReference type="EC" id="1.1.1.408"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PdxA family. PdxA2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.408" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196423.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q3V818"/>
    <dbReference type="SMR" id="Q3V818"/>
    <dbReference type="STRING" id="292459.STH2300"/>
    <dbReference type="EnsemblBacteria" id="BAD41285">
        <property type="protein sequence ID" value="BAD41285"/>
        <property type="gene ID" value="STH2300"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2300"/>
    <dbReference type="eggNOG" id="COG1995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040168_0_1_9"/>
    <dbReference type="OMA" id="TAQVVMM"/>
    <dbReference type="OrthoDB" id="1414545at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="PTHR30004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00557">
        <property type="entry name" value="pdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000211916" description="Putative D-threonate 4-phosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P19624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P58718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="335" mass="36102" checksum="C6B4D45E97B0DEDE" modified="2005-10-11" version="1">MDTRPLIGITMGDPASIGPEIAAKALANPEIYALCRPLLIGDSRVMARAFETTGVKLNLNPVATPAEGKYAHGTVDLIDLPVVDMGTLQWGQVQAQAGAAAFAYIKRSIELALEGAVDAVTTGPINKEALKAARIDFIGHTEIFGELTGSHDPLTMFETKGLRIFFLTRHVSLAQACRMITRDRVLDYIRRCTAALEQLGLVRPKLAVAGLNPHCGEHGLFGDEEVREIEPAVQQAQAEGYNVSGPHPADSVFWHAAQGRFDAVLSLYHDQGHIAAKMYDFERTVSITAGLPFLRSSVDHGTAFDIAGTGRASAVSLEEAIRVGAKYAAAFRRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J32</accession>
    <name>Q67J32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SpoIIIJ-associated RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">jag</name>
        <name evidence="7" type="ordered locus">STH3337</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197448.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3337"/>
    <dbReference type="EnsemblBacteria" id="BAD42318">
        <property type="protein sequence ID" value="BAD42318"/>
        <property type="gene ID" value="STH3337"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3337"/>
    <dbReference type="eggNOG" id="COG1847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042512_0_0_9"/>
    <dbReference type="OMA" id="MLDIASW"/>
    <dbReference type="OrthoDB" id="1304273at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02414">
        <property type="entry name" value="jag_KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02644">
        <property type="entry name" value="R3H_jag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038008">
        <property type="entry name" value="Jag_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038247">
        <property type="entry name" value="Jag_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039247">
        <property type="entry name" value="KhpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032782">
        <property type="entry name" value="KhpB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034079">
        <property type="entry name" value="R3H_KhpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35800">
        <property type="entry name" value="PTHR35800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14804">
        <property type="entry name" value="Jag_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01245">
        <property type="entry name" value="Jag_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="SSF82708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="R3H" evidence="6">
        <location>
            <begin position="139"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="205" mass="22640" checksum="C70C6832DEF93C96" modified="2004-10-11" version="1">MRSVQRSGRTVDEAVAAALEELGVPSDRVTVEVLDEGKGGFLGIGARPATVRVTVKESRAERVEAFLGDVCEAMGVGVRMEIREDGEYIHVDITGQEAGILIGHHGQTLDALQYLTNLVAARSDRHGPRILLDVEGYRKRRTETLTNLARRLAERVVRTGERAVLEPMSAQERRVIHLALQDFPGVTTASEGQDPFRRVVIYKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J48</accession>
    <name>RS6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsF</name>
        <name type="ordered locus">STH3321</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197432.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J48"/>
    <dbReference type="SMR" id="Q67J48"/>
    <dbReference type="STRING" id="292459.STH3321"/>
    <dbReference type="EnsemblBacteria" id="BAD42302">
        <property type="protein sequence ID" value="BAD42302"/>
        <property type="gene ID" value="STH3321"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3321"/>
    <dbReference type="eggNOG" id="COG0360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113441_5_3_9"/>
    <dbReference type="OMA" id="ITEASPM"/>
    <dbReference type="OrthoDB" id="1776954at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000176856" description="30S ribosomal protein S6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <sequence length="95" mass="10930" checksum="01B7BB960317E845" modified="2004-10-11" version="1">MRKYEMMVIARPDLDEAGLQALSDKIAELITSNGGTVESQDAWKKQRLAYEIKHLREGFYSVFNFTGEPRTANELNRVLKITDEVVRFLIVRPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J57</accession>
    <name>Q67J57_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3312"/>
    <dbReference type="EnsemblBacteria" id="BAD42293">
        <property type="protein sequence ID" value="BAD42293"/>
        <property type="gene ID" value="STH3312"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3312"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1069305_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="1">
        <location>
            <begin position="148"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="29759" checksum="67B81596AA89535C" modified="2004-10-11" version="1">MLRDRLRQISRPAHGKVFLRWEFMRSDRARRRFRARRLRRDLMALVVFLFIFALNGMQGRLEAAGVPQALGPVEPTIPEHDLMLAQRRAEAARRSASLVAREAEEAAADLTAAQRQLARQADAERRRDLERIADLVPPEFRQMVLDLSAQYGVDPRLVAAVGHVESRWNPRTVGTHNDTGLMQILPGTAQWIASRLGWTEYDLFDPWTNLHMGIWYLQALYREYGSWDKALAAYNGGPRQAHLGADHPYVGRVMRVYAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J86</accession>
    <name>Q67J86_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197395.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3283"/>
    <dbReference type="EnsemblBacteria" id="BAD42264">
        <property type="protein sequence ID" value="BAD42264"/>
        <property type="gene ID" value="STH3283"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3283"/>
    <dbReference type="eggNOG" id="COG2414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_9"/>
    <dbReference type="OMA" id="WHPCWNC"/>
    <dbReference type="OrthoDB" id="332680at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFOR_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="604" mass="64347" checksum="6B8453D9E40FAC34" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA8</accession>
    <name>Q67JA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative chromosome replication initiation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3260"/>
    <dbReference type="EnsemblBacteria" id="BAD42242">
        <property type="protein sequence ID" value="BAD42242"/>
        <property type="gene ID" value="STH3260"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3260"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_0_0_9"/>
    <dbReference type="OMA" id="CKDTGFI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="196"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="341" mass="39310" checksum="ECFFCA825437AF4B" modified="2004-10-11" version="1">MIATMRSSLSDLTIERAKRREARIRQLVQERDEREAAIFARIPRLAEIKALQAEIGLDLARLYLKRPTRFKMTFEELRDWSLRLSREREELLRQHGIDPRELEVHWDCPKCQNTGWIRDTEAGETVAPPRKCSCLIQEEIEDLYRVAGLTGPLREHTFDRFDLTVYPAECRKHMAGVRDYCRRFAEAVAEGRCRTSLLLQGAVGLGKTFLASAIGNYVIAARRSVLYFTLAEFVDLIRAEKFEDGEDARASAQWLLEADLVILDDLGAEKVTEFVAQELFNLINLRLNRQMPMVVSTNLRASELFDTYGERIASRLLYGFEALTLEGEDVRRVLRLRAGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD7</accession>
    <name>Q67JD7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MazG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3231"/>
    <dbReference type="EnsemblBacteria" id="BAD42213">
        <property type="protein sequence ID" value="BAD42213"/>
        <property type="gene ID" value="STH3231"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3231"/>
    <dbReference type="eggNOG" id="COG3956">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038356_2_1_9"/>
    <dbReference type="OMA" id="HPHIYGD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011551">
        <property type="entry name" value="NTP_PyrPHydrolase_MazG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30522">
        <property type="entry name" value="PTHR30522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00444">
        <property type="entry name" value="mazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="130"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="365" mass="40689" checksum="E859AD92C5607B26" modified="2004-10-11" version="1">MADMEDLLRAFDIHLADGLEVRPAARLRSWAGPAELPLLIPGVLPGDLGAVQRALLPYYPADHPVRVRAEGRVHEVPLDGLAGQNWGEYRIDLFLAPPARAQRERWPLDPLVEVMDRLRGPDGCPWDREQTHETLRRYMLEEAYEAVEAIDDGDPDHLCEELGDVLLQVVFHAQIAREAGRFDMRDVVAGITEKLIRRHPHVFGDASAETAEEVTRRWDAIKRAERGGTGEESLLSGVSRSLPALSRACELQKRAAGVGFDWEDAEGPAAKVREELAEVLGAPPGEREAEVGDLLFAAVNLARKLGVDPELALTRASAKFEQRFRYVERRLAEKGLRPADVSLAEMDALWEEGKRVELRKKYGGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH4</accession>
    <name>HUTH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine ammonia-lyase</fullName>
            <shortName evidence="1">Histidase</shortName>
            <ecNumber evidence="1">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutH</name>
        <name type="ordered locus">STH3194</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-histidine = NH4(+) + trans-urocanate</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JH4"/>
    <dbReference type="SMR" id="Q67JH4"/>
    <dbReference type="STRING" id="292459.STH3194"/>
    <dbReference type="EnsemblBacteria" id="BAD42176">
        <property type="protein sequence ID" value="BAD42176"/>
        <property type="gene ID" value="STH3194"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3194"/>
    <dbReference type="eggNOG" id="COG2986">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014801_4_0_9"/>
    <dbReference type="OMA" id="CAPQVAG"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00229">
        <property type="entry name" value="His_ammonia_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="PTHR10362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000161043" description="Histidine ammonia-lyase">
        <location>
            <begin position="1"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Ser)" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="cross-link" description="5-imidazolinone (Ala-Gly)" evidence="1">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00229"/>
        </source>
    </evidence>
    <sequence length="507" mass="53709" checksum="4343495E574F09B5" modified="2004-10-11" version="1">MEAVELGAHLTLPEVVAVARHGARVVLTPEVRQRVARASEMVERLVRERRPVYGITTGFGKFSDVPISAEQTEALQRNLLMSHACAVGEPLAAEVVRAMLLLRAQALSRGHSGIRAETLEMLVAFLNLGLTPVVPEQGSLGASGDLAPLAHMSLPLIGLGEAVVNGERLSGAEALQRVGLRPLTLTAKEGLALINGTQAMTALGSLGLHDAQVLLKTADIAAAMTAEALGAIPAAWDPRVQALRLHTGQQAAARNLRRLTEGSRLTTRPGQMRTQDPYTLRCLPQVHGASRTAIEHVAQVLDWEMNAVTDNPLLFPDDDEVISGGNFHGQPVALALDYLAIAVAELGDIAERRIERLVNPQLSGLPAFLTRNGGVHSGLMITQYTAASLVSENKVLAHPASVDSIPSSANQEDHVSMGTTAARKARQVIANVRRVLAIELLCAAQALEFVGPERLAPATRAAYAAIRERVAPLSGDRVLAPDIEALAELVHNGELVAAVEAVAGPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL9</accession>
    <name>Q67JL9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197262.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3149"/>
    <dbReference type="EnsemblBacteria" id="BAD42131">
        <property type="protein sequence ID" value="BAD42131"/>
        <property type="gene ID" value="STH3149"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3149"/>
    <dbReference type="eggNOG" id="COG0348">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032118_2_1_9"/>
    <dbReference type="OrthoDB" id="1230380at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032879">
        <property type="entry name" value="FixG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13746">
        <property type="entry name" value="Fer4_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11614">
        <property type="entry name" value="FixG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="408" mass="45342" checksum="AADFE5BDA18C2746" modified="2004-10-11" version="1">MKSRLGKVQTVIRRYRRASQLTVLALLIVSPFVNLFRFDLPSMSLYLFGTRLWVKHTFVFFMLLVTVIYVIIAASLLFGRVFCGWVCPQNLFNELTRSWDRRFGRAGSTLLSVVISLFGAFVVRSYFSDGRALLAQYLSGQVPPGPTVFILGFTLFFTMAMAWWRTSVCRVACPYGHLQSIISNADTMHLQVFNLPGNRDICATCGLCAEICHMGVDPRTPEQKHCVVCGDCLDACALVSGARKVPRVLNFTIGSGDSAVALRDRGRLWQNVRQLAPRLVLPLILTVILGSLSAYMLAHRPRVDLVVAKNHRQVLAAGSPISSGNVMAVSIINLGNEGDRFHLYVEGLPDNWAALERSEIELEPGERASVTLRVHPTERIVGTHRFTVRVVGERSGAEASFDTVHVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT5</accession>
    <name>Q67JT5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3083"/>
    <dbReference type="EnsemblBacteria" id="BAD42065">
        <property type="protein sequence ID" value="BAD42065"/>
        <property type="gene ID" value="STH3083"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3083"/>
    <dbReference type="HOGENOM" id="CLU_2756367_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="70" mass="7750" checksum="1BD0EE0E0A67FE92" modified="2004-10-11" version="1">MVPKNGAEGKSARRRAPPAAEVDESPLIEAARAGVDGAFDSLVRPHLDRAYRITVPFENVREFAQRFVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K16</accession>
    <name>Q67K16_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagellar assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fliH</name>
        <name evidence="4" type="ordered locus">STH3001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3001"/>
    <dbReference type="EnsemblBacteria" id="BAD41984">
        <property type="protein sequence ID" value="BAD41984"/>
        <property type="gene ID" value="STH3001"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3001"/>
    <dbReference type="eggNOG" id="COG1317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_936664_0_0_9"/>
    <dbReference type="OMA" id="VIDPRIN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018035">
        <property type="entry name" value="Flagellar_FliH/T3SS_HrpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02108">
        <property type="entry name" value="FliH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FliH" evidence="3">
        <location>
            <begin position="158"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="32075" checksum="AF7DBF00A0EF5BBB" modified="2004-10-11" version="1">MSTSRSGKLPPGVEVRAPSWPQLPPPPPPSPPPPSPPKPARRPAPPTPEELEAEKKAAESVLDEARLEAARILQSAAEEAERAEAAAREEGFQQGRREGLEAGRAEVEALRREAELALENARLQGENLRQAAEKEAAVTRLEAEKAAEKLLADAREEAQRILEEARQRAERLVQERLEQSQEALVQLAVAAAARLVQGHLAVEPQSVVRMVAAGLRLVKDAPATVRVNPEDLPLLEAQRAVLERELGAGALTLTADPGMQRGSYMVQTPVGQVDGDLEHQIERMEAAMRAALGGSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA5</accession>
    <name>Q67KA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197027.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2910"/>
    <dbReference type="EnsemblBacteria" id="BAD41893">
        <property type="protein sequence ID" value="BAD41893"/>
        <property type="gene ID" value="STH2910"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2910"/>
    <dbReference type="eggNOG" id="ENOG502ZBQP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117532_0_0_9"/>
    <dbReference type="OMA" id="EIGFWVL"/>
    <dbReference type="OrthoDB" id="1147201at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20594" checksum="892C2075C7FDFE2B" modified="2004-10-11" version="1">MSPIAYLIVGIEIGFWVLVISGLAVRYVLNRPRLSALLLAMTLVLDGILLVVTTRDVLVNGATPTLAHGIAAFYLSVSVTFGKRIVRWADERFQYYVLKRGPRPRQLYGYEHARYNFSGSMLYLLAYLLGGGYLGLLIYLIGDLGRSVALLRVLGFWTVSLVIDMAISVSYFIWPRRPESESAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC2</accession>
    <name>Q67KC2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197010.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2893"/>
    <dbReference type="EnsemblBacteria" id="BAD41876">
        <property type="protein sequence ID" value="BAD41876"/>
        <property type="gene ID" value="STH2893"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2893"/>
    <dbReference type="HOGENOM" id="CLU_1065298_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269515" evidence="2">
        <location>
            <begin position="20"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="261" mass="26916" checksum="1DFEB2458D37D98E" modified="2004-10-11" version="1">MARLLTAELLLLALTAAFAAAGVPWLAGQPPAWPLLSVAFLMALAFSALYALANLAARRLRQPALLAAGLVALASVAVDALRRGPFAWGEGLALAAGLVLSALGGALGRGAGAGLWAYAAPLAAVRYALVPLAGSGEPVRALAVQSAAFYLFLRGLVRLAFPTSAEGNPSAGPLVHRPVPDRVVGLVEATARRRALPYATREDGSRDEEAVAVRCPRAKAAELAERLRAVLEGHPVTVTQGVQAGDEVELVIRVVPPPRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG2</accession>
    <name>Q67KG2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MutT-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2851</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2851"/>
    <dbReference type="EnsemblBacteria" id="BAD41836">
        <property type="protein sequence ID" value="BAD41836"/>
        <property type="gene ID" value="STH2851"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2851"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1401834_0_0_9"/>
    <dbReference type="OMA" id="VKTTKWR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="22338" checksum="02BC7852E7C159C2" modified="2004-10-11" version="1">MRVHSPAGARSRIGGRAVREEGPYRREESALAEQFILGLGLVEDEDRLVIVRNRWAVGEVWSLPGGRLEVGESLTDCVVREVQEETGLLVAPVELAYVQDTHNLVHDQHFLVHVFSCRLVAGTLRVPEHDEYVVDVRWVKRDEVARYITWPTYRDPLLAYLAGHERRYWLDRDGYRPELGKGPDRKGKEENGWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG9</accession>
    <name>Q67KG9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2844"/>
    <dbReference type="EnsemblBacteria" id="BAD41829">
        <property type="protein sequence ID" value="BAD41829"/>
        <property type="gene ID" value="STH2844"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2844"/>
    <dbReference type="eggNOG" id="COG4496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147939_1_0_9"/>
    <dbReference type="OMA" id="DECYRFF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000831">
        <property type="entry name" value="Trp_repress"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038116">
        <property type="entry name" value="TrpR-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013368">
        <property type="entry name" value="YecD_YerC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40080">
        <property type="entry name" value="PTHR40080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01371">
        <property type="entry name" value="Trp_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012508">
        <property type="entry name" value="YerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02531">
        <property type="entry name" value="yecD_yerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="110" mass="12390" checksum="F7744DADC0413920" modified="2004-10-11" version="1">MLGGNPCMPVNEKLRDELTDKLFEAMLTLKTVEEFYRFFEDIATIGEIKALAQRLEVAKMLDAGMTYEDIVAATGASTATISRVKKCLNYGADGYRIVLERTRNEYKKQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH6</accession>
    <name>HISX_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol dehydrogenase</fullName>
            <shortName evidence="1">HDH</shortName>
            <ecNumber evidence="1">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisD</name>
        <name type="ordered locus">STH2837</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196956.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KH6"/>
    <dbReference type="SMR" id="Q67KH6"/>
    <dbReference type="STRING" id="292459.STH2837"/>
    <dbReference type="EnsemblBacteria" id="BAD41822">
        <property type="protein sequence ID" value="BAD41822"/>
        <property type="gene ID" value="STH2837"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2837"/>
    <dbReference type="eggNOG" id="COG0141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_3_9"/>
    <dbReference type="OMA" id="MVTGPGN"/>
    <dbReference type="OrthoDB" id="935289at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135861" description="Histidinol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <sequence length="439" mass="46628" checksum="45D8E26BC3568EAA" modified="2004-10-11" version="1">MLKVWEPQAFLEFLNRRRTEFYPEIEAQVRAILERVRREGDGALYAFTRQFDGADLEATGLRVTEAEYREAEAAVSAAFRAALQVAVENIAAFHRPQVPTSWFTTRPDGTIVGQRVTPVDRAGVYVPGGSAPLFSCLLMTAIPAVVAGVPEVIVCTPPDRNGRIDPHMLVAARAAGVKDVYKVGGAQAIGAMAYGTATVPRVDKIAGPGNYYVTLAKKLVFGPVGIDMLAGPTEVMAVDDGSVDAEWLAADLLSQAEHPNGMVILVTTAGPERIAAVGAAMARHTAALPRAETIRRSVAELGAALAVDTLEEAADLVNAVGPEHLEVGVADPWAFLPLVRHAGSIFLGRWTPEAMGDYIAGPSNVIPTEGTARYASPVCVETFIKRSAVTCYSEAAFRAQAPHAVRLALTEDLLAHAASMQIRLAKPDGESPSEGREAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ1</accession>
    <name>GATC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name type="ordered locus">STH2822</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196941.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KJ1"/>
    <dbReference type="SMR" id="Q67KJ1"/>
    <dbReference type="STRING" id="292459.STH2822"/>
    <dbReference type="EnsemblBacteria" id="BAD41807">
        <property type="protein sequence ID" value="BAD41807"/>
        <property type="gene ID" value="STH2822"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2822"/>
    <dbReference type="eggNOG" id="COG0721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_1_2_9"/>
    <dbReference type="OMA" id="VTPMAMK"/>
    <dbReference type="OrthoDB" id="1780195at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="PTHR15004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000076201" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <sequence length="96" mass="10843" checksum="18406BD93568F7E7" modified="2004-10-11" version="1">MAVTRDEIQRVARLARLSLSEEEIETFAEQLNRILEHVERINRLDVADVPPTYHGVALQHPFREDKPLPSLDREAVLALAPEAEAGCYKVPKITEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS6</accession>
    <name>Q67KS6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2736</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2736"/>
    <dbReference type="EnsemblBacteria" id="BAD41721">
        <property type="protein sequence ID" value="BAD41721"/>
        <property type="gene ID" value="STH2736"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2736"/>
    <dbReference type="eggNOG" id="COG1705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_396335_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00047">
        <property type="entry name" value="LYZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269248" evidence="2">
        <location>
            <begin position="31"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="LYZ2" evidence="3">
        <location>
            <begin position="545"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="695" mass="75907" checksum="62DC405273787AF8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU6</accession>
    <name>Q67KU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2715"/>
    <dbReference type="EnsemblBacteria" id="BAD41700">
        <property type="protein sequence ID" value="BAD41700"/>
        <property type="gene ID" value="STH2715"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2715"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_9"/>
    <dbReference type="OMA" id="PKVKYLN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="34417" checksum="FC6B28DB4A1311B8" modified="2004-10-11" version="1">MGCVMRTVLITGGAGFVGSHVVERFLAEGLRVVVVDNLTTGVREHVPPGAEFHNIDILTPEFTSLVGKVKPDTIVHLAAQVSVAVSVRDPVLDADVNVGGTLRVLEAAREHQVPNFVFSSSAAVYGIPSSLPVTEDAPFSPLSPYGIAKVAAEGYIRAYCFLHGLKAVVMRYSNVFGPRQKAAGDGGVVANFVEAILRGHPPVFFGDGGQTRDFIYVKDVADATLKAIDYLDKSGTSEYLVVNISSGVETSLRTLYTLLCELVKQAPEPILTPPREGDIRHSCLDNRKAREYLGWLPGYSLEQGILETVTAARLAGQGARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW0</accession>
    <name>Q67KW0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196823.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2701"/>
    <dbReference type="EnsemblBacteria" id="BAD41686">
        <property type="protein sequence ID" value="BAD41686"/>
        <property type="gene ID" value="STH2701"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2701"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_11_0_9"/>
    <dbReference type="OMA" id="ALWFEPT"/>
    <dbReference type="OrthoDB" id="362657at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017895">
        <property type="entry name" value="HTH_IS408/IS1162_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50532">
        <property type="entry name" value="HTH_IS408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH IS408-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="124"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="508" mass="57825" checksum="A5A83EE375DFF988" modified="2004-10-11" version="1">MRKIREILRLKWEVGLSARQIARSLSISHSTVLDMLRRLECSGLSWPLPNIDDAELEAKLYPGNPQGLVERPLPDMAYIHRELARKGVTLQLLWWEYKQNHPDGLQYSQFCQRYRQWRSKLDVVLRQPHRAGEQMQVDFAGDTVPIHDRETGEVWQAPIFVAVLPASNYTFAKAYPAQDLRSWIKAHCDALEFFGGVPEIIVPDNPKAGVTRACRYEPDLNPAYQEMAAHYGAAVIPARPRSPRDKAKVENAVLVVERWILAALRNHTFFSLHDANRAIREAVARLNDRPFQKLEGTRRSLYETLDKPALRPLPPQRYEFAEWRKAKVNLDYHVEVDRNYYSVPYPLVGETVDIRLTDGLVEILHRGKRVACHPRATGRGRSVTQIQHMAASHRRHREWTPSKLRRWAESVGPHTAQLVVTILEEKPHPEQGFRSCLGIIHLARKYGSDRLEAAAARACAARTFSYSSVKSILAHNLDQAPLPGPPDNQATPVHPNLRGPDYYAGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L69</accession>
    <name>Q67L69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA helicase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196714.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2592"/>
    <dbReference type="EnsemblBacteria" id="BAD41577">
        <property type="protein sequence ID" value="BAD41577"/>
        <property type="gene ID" value="STH2592"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2592"/>
    <dbReference type="eggNOG" id="COG1112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_304015_0_0_9"/>
    <dbReference type="OMA" id="NLWNVAI"/>
    <dbReference type="OrthoDB" id="616258at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="978" mass="105735" checksum="CB6D918F201B3163" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L73</accession>
    <name>Q67L73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative pyrroline-5-carboxylate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196710.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2588"/>
    <dbReference type="EnsemblBacteria" id="BAD41573">
        <property type="protein sequence ID" value="BAD41573"/>
        <property type="gene ID" value="STH2588"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2588"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1194395_0_0_9"/>
    <dbReference type="OMA" id="CICYGDE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="23670" checksum="AAB9D14AB4923A65" modified="2004-10-11" version="1">MNDKIAIIGAGPLASILAHRIPTSVRKVIIGRPKAAAVALADEVGGIASDQASAVRGCRVVFLAVPTPDVAQALADIRPHLSPEALVVNTAVDVPTADLADDRLHVGAAKIIGHPQELEQGARAVVVLDHVGPADAELLTALLADLGPVIRGDERTVEAAARALSEVLAGARDELRRRLAEAGLPADVVPAAIAAAAPGVLRAMALSEDNAAERGIRRHAGAGETAASRVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L84</accession>
    <name>Q67L84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter ATP-binding protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2577"/>
    <dbReference type="EnsemblBacteria" id="BAD41562">
        <property type="protein sequence ID" value="BAD41562"/>
        <property type="gene ID" value="STH2577"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2577"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2496748_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="86" mass="9606" checksum="E669338DFA76E564" modified="2004-10-11" version="1">MREPELLVFDDISSALDVETEQLLWRRLLARGAACVAVSHRPAVLRQAHRIIVLREGRVVGCGRLEELLHSCPEMRDLWFAAGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC4</accession>
    <name>Q67LC4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MaoC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196660.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2537"/>
    <dbReference type="EnsemblBacteria" id="BAD41522">
        <property type="protein sequence ID" value="BAD41522"/>
        <property type="gene ID" value="STH2537"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2537"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_3_2_9"/>
    <dbReference type="OMA" id="PGPGCIY"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17593" checksum="971D25EB70B2DC29" modified="2004-10-11" version="1">MGLFWDNLGMSFDQLYVGQKATYSRKVSKQDVLQYMSLSGDLNPLYGDATYAGRTTYGHPIVPANMLAGFAMGAVAAVLPGLGSITHAHSYRLLHPPRIGDEVTAEMEIVELHPESQRVTIQYVIRDQDGRELQHGTMEVEPPQQLKPMLRHDYETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC5</accession>
    <name>Q67LC5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fumarate hydratase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2536</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196659.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2536"/>
    <dbReference type="EnsemblBacteria" id="BAD41521">
        <property type="protein sequence ID" value="BAD41521"/>
        <property type="gene ID" value="STH2536"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2536"/>
    <dbReference type="eggNOG" id="COG1951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_9"/>
    <dbReference type="OMA" id="NIECHSH"/>
    <dbReference type="OrthoDB" id="972093at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="30082" checksum="4F4D41AEB1DF5E33" modified="2004-10-11" version="1">MREIHVDQVADAVARLVQEANFKLSDDVVRALSQAQSLEEAPVGQTVLLQIVENYTIAETEQVPMCQDTGVALVFLEIGQDVHLVGGDLYEAINRGVARGYTEGRLRMSMLNDPIKRVNTRDNTPAMIYVDIVPGDQLRIKVDTKGGGSENMSQLKMLPPSAGWEGAKRFIVETVAAAGPNACPPLVVGVGIGGNFDKCALLAKKALLREVGAPNPDPEWAAREQELLTEINKLGVGPMGLGGTVTALAVHIEVMGCHITALPVAVNIECHSHRHKEVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE3</accession>
    <name>Q67LE3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative thiamine biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2518</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2518"/>
    <dbReference type="EnsemblBacteria" id="BAD41503">
        <property type="protein sequence ID" value="BAD41503"/>
        <property type="gene ID" value="STH2518"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2518"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_5_2_9"/>
    <dbReference type="OMA" id="EWTEILV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="PTHR31528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="27"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="317" mass="34319" checksum="2CA1ECC6916DEE72" modified="2004-10-11" version="1">MAGCGGKPQNAYQKEPATPAAPETVGQLKIGQIPTIDGLPFWVAASKDYYRQQGVEVELVRFNSAAERDAALASGQIDGTLTDIMGAVSLHDNGTPLQITSINLGATIEEGPFAIVSAPGSGITKPEQLKGADIAIATNTIIHYVTEKLLLENGIALDEVELVSIPQIPLRFENLMSGNVKAATLPEPLLSLAVHKGGTVVLNDAEAKRNYSQSVIVFREDAVKEKAEAIKRFFVAYNMAVIDIDRDPEAYKELLATEANLPPEIKDSWKVITFSPAQAPGRTEVEDVVEWLLEKGYISKRVSYDEIVNTTLYPQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH3</accession>
    <name>Q67LH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2488"/>
    <dbReference type="EnsemblBacteria" id="BAD41473">
        <property type="protein sequence ID" value="BAD41473"/>
        <property type="gene ID" value="STH2488"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2488"/>
    <dbReference type="HOGENOM" id="CLU_1676962_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17630" checksum="366AA9DFEE49667C" modified="2004-10-11" version="1">METFSQASVPPANYGKYFIDGGICTMADTEPKAQGNEAYYATARTLGTGTTSSPTMKIWRVMDDYLARFGRAVSASELAQETGLTVEQIDDIFAQEYYQKHYGFRRFDSLEEWRAWAMETGVLYNPELHRPDPEEPEEVPAEQGEPSEEPSPESPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM5</accession>
    <name>Q67LM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2436"/>
    <dbReference type="EnsemblBacteria" id="BAD41421">
        <property type="protein sequence ID" value="BAD41421"/>
        <property type="gene ID" value="STH2436"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2436"/>
    <dbReference type="eggNOG" id="ENOG5033AZX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120980_1_0_9"/>
    <dbReference type="OMA" id="FIFTITA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025671">
        <property type="entry name" value="HXXEE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13787">
        <property type="entry name" value="HXXEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="19276" checksum="D159FF511F2A2A01" modified="2004-10-11" version="1">MIAPQGCHGGQVSGMTRRTLFWLGPLVFLVHDLEEVFLARAWVEQNLLLIAGTPFEPVVEAMGYEPGKFGLVVALATVVYGVIAWSAARACQPGLSLNLYVATVLTLFVNVITHVGQALLLRMYTPGVITAVLVFLPYTVAAFRMLRAQRLLTATTWKTSPLMGIGMLAALFGLMIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR1</accession>
    <name>Q67LR1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2400"/>
    <dbReference type="EnsemblBacteria" id="BAD41385">
        <property type="protein sequence ID" value="BAD41385"/>
        <property type="gene ID" value="STH2400"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2400"/>
    <dbReference type="HOGENOM" id="CLU_1776517_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="49"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="16524" checksum="E129F3640B0C3F9B" modified="2004-10-11" version="1">MHGTKGGYGCASICDRLKTGTAFGRRRRKEDPPVSQSVMTERLRVEEAVTRFFHMCNSHCPGNVAPLLTADVELTAERTASGQEGVHSYLVWLWQTYPDLTFRVEHVLVDGPAAAAEVTAEAQGERRARCVIFHFRDGLIRRMRWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M14</accession>
    <name>Q67M14_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2296</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2296"/>
    <dbReference type="EnsemblBacteria" id="BAD41281">
        <property type="protein sequence ID" value="BAD41281"/>
        <property type="gene ID" value="STH2296"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2296"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041737_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="20"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="177" mass="18461" checksum="32FFD47965A63C08" modified="2004-10-11" version="1">MPPFLPGDTRRALRSLRAPSPLSRGQGGIALGLLSTCAMVLLKPMGVSTVFPSTLGILLKPLFPGFVEGDAYLQTIPLSIGWEHMLMLGIPLGGYLAYRLAGEGRDGQPSSGLSTTSPLLAFVGGFLVLFGARMAGGCTTGHVLSGMSQLSVSGFLFAAAVFAGGIPTALLLKRRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M36</accession>
    <name>Q67M36_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2274"/>
    <dbReference type="EnsemblBacteria" id="BAD41259">
        <property type="protein sequence ID" value="BAD41259"/>
        <property type="gene ID" value="STH2274"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2274"/>
    <dbReference type="HOGENOM" id="CLU_1554520_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="18650" checksum="8739B3C580E5E2C2" modified="2004-10-11" version="1">MVTIRVLAALLIVGSLLLTACDYGGEAYPTQLNELVQMTNDLSESIAVLLQADLVTTAEASSTKRLVTGLQSQVHDAVKALEKEKISVSLFWLNNMLEQLDLVVTAVHARTSQGGDVLTPDERTALSNGLSLLSEMGRTALDLQGEEMTASRRLSETLRAWDELASDMPSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M97</accession>
    <name>Q67M97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196336.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2211"/>
    <dbReference type="EnsemblBacteria" id="BAD41196">
        <property type="protein sequence ID" value="BAD41196"/>
        <property type="gene ID" value="STH2211"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2211"/>
    <dbReference type="eggNOG" id="COG1470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051163_0_0_9"/>
    <dbReference type="OMA" id="GMAVMRF"/>
    <dbReference type="OrthoDB" id="601520at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018905">
        <property type="entry name" value="A-galactase_NEW3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10633">
        <property type="entry name" value="NPCBM_assoc"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269534" evidence="2">
        <location>
            <begin position="29"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="NPCBM_assoc" evidence="3">
        <location>
            <begin position="43"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="NPCBM_assoc" evidence="3">
        <location>
            <begin position="153"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="NPCBM_assoc" evidence="3">
        <location>
            <begin position="264"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="39999" checksum="C1B80A134C51C3CA" modified="2004-10-11" version="1">MSCRTMRWGTALAAAALALLTAALPAQAAQLNFSTRYPGIVVKPGESLSLTLNLSGDGGVADLSVRDLPEGWEPPVFRGSGYPVNQVYVPPGGSESVTLSLKVPDRAPEGTYRVVLAASSGGEVVELPLNITVTAGATGRASLRTDFPSIQAQAGTTYTFTVTLSNDGDLEEMFALSAAPPEGWNVVFQVSGKQVGTIPVEGNASQSISVSVTVPNEIEAGTYTVPIFARSGSAEARLDLSLEVLGKYALDLTTPDGRLSFDAVAGRKNGVTLEVKNTGTTEITGINFASTLPPNWTATFSPDSIDALAPGESRQVTVEVTPNSKALAGDYLVTLRARSDRASDSAEFRVAVQTPTLWGLAGVGVLAVVGGGLYWVFRTFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI1</accession>
    <name>Q67MI1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Penicillin-binding protein 1A</fullName>
            <ecNumber evidence="8">2.4.1.129</ecNumber>
            <ecNumber evidence="7">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">STH2127</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="8"/>
    <dbReference type="EC" id="3.4.16.4" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2127"/>
    <dbReference type="EnsemblBacteria" id="BAD41112">
        <property type="protein sequence ID" value="BAD41112"/>
        <property type="gene ID" value="STH2127"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2127"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_7_9"/>
    <dbReference type="OMA" id="AVQMPYI"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="21">
        <location>
            <begin position="85"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="20">
        <location>
            <begin position="344"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="651"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="770"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="828"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BAD41112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="857" mass="90870" checksum="4ED960015DF76120" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK3</accession>
    <name>Q67MK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spore germination protein KB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gerKB2</name>
        <name evidence="7" type="ordered locus">STH2105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2105"/>
    <dbReference type="EnsemblBacteria" id="BAD41090">
        <property type="protein sequence ID" value="BAD41090"/>
        <property type="gene ID" value="STH2105"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2105"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_821173_0_0_9"/>
    <dbReference type="OMA" id="IAREAWW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004761">
        <property type="entry name" value="Spore_GerAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34975">
        <property type="entry name" value="PTHR34975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03845">
        <property type="entry name" value="Spore_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="338" mass="36872" checksum="1E94FFBA3A52D4B3" modified="2004-10-11" version="1">MLLESAGQEAWITVLILGVYALVVALAVVWIACRFPDRDPLKAIRCTLGSWATYPIVLLYCAFNVLIYAMSLSDVQTFTKILLLPDTPAWVLAVMIALFTIYTVLQGVEPIARTAYGVLVPLVLVILVLPFGVGKEIRLLQIDPFLWKGFSGVLRGAWLALPWAGQSLIVLSLMRHLSLRVNPYKWTLIGVGSAHLALAIMVLLISLVFGSILPSRLLYPGHELFAIVALTESIERIQAGIMVLWLCGSLINITLHLYVAVEEGSLALGIQRRGWITLILGAAGVFLAQWAGGALYRRSIAREAWWIGMHIAGQLAVLALLGLATLLIRMRQGAGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS6</accession>
    <name>Q67MS6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2032"/>
    <dbReference type="EnsemblBacteria" id="BAD41017">
        <property type="protein sequence ID" value="BAD41017"/>
        <property type="gene ID" value="STH2032"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2032"/>
    <dbReference type="HOGENOM" id="CLU_1481274_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269450" evidence="2">
        <location>
            <begin position="33"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="182" mass="19466" checksum="9FD20E2FA92DCFE9" modified="2004-10-11" version="1">MRRRLDGRRLPGALAALLVTLILLAPARPALAHAVDLFGYVSVDEQGTVTARLVDVYGGLVEGQRVELYARPQGGRAGPPVVMEELEPGTYRGRAVPNRAGPYEVVIDLPLAGDLHRITLPVEPGVPVAERYVIMEQIEPLLTWTRVLFAAAAVVLAVGSVIAWRRRPGGPEPEAAGGGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS8</accession>
    <name>Q67MS8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF218 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196156.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2030"/>
    <dbReference type="EnsemblBacteria" id="BAD41015">
        <property type="protein sequence ID" value="BAD41015"/>
        <property type="gene ID" value="STH2030"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2030"/>
    <dbReference type="eggNOG" id="COG2949">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051474_0_1_9"/>
    <dbReference type="OMA" id="YDEPDAM"/>
    <dbReference type="OrthoDB" id="1879123at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="52"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="24637" checksum="47F6952DA9BD74BB" modified="2004-10-11" version="1">MRGSGGFARGLARWLVRLLAVGTAAVLAINALVYFPGRSRIITADAARPAQVALVLGAGVYADGRPTPMLRDRLETALALYEAGKARKLLLSGDHSRPDYDEVNAMRRYLQERGVPPEDLFLDHAGFDTYDSMYRAAAIFQVDDVIVVTQGFHLPRALWIAARLGLDAQGVAADQRRYGVEQYYAAREFAARVKAFAEVAVRRRPAFLGPVIPITGDGRATHDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT0</accession>
    <name>Q67MT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2028"/>
    <dbReference type="EnsemblBacteria" id="BAD41013">
        <property type="protein sequence ID" value="BAD41013"/>
        <property type="gene ID" value="STH2028"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2028"/>
    <dbReference type="HOGENOM" id="CLU_1089593_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="27279" checksum="53F8B2F656EA429D" modified="2004-10-11" version="1">MAMPYELRDLAPALEYAGRHPEAGFALLRSLQSGLRPGLAAIVDDPERVTAVMLVHRPDWRKRDPLPTHIQLDAETAGAALRLLAWVPPGAEVRLTTYRPWLHELALGVLEGARTVQHVLCVADAQRFRPDPGTDLAVEIGADQVAQAGHDGAVGPEEGDRLFGVVREGRLLACAALGPPENGYAPVRCLFPASPEGRQGCGAAALSAAVQAALQAGHRVLCRLPASELPLLHLAARLGLSPVCREWSAEGRVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW5</accession>
    <name>Q67MW5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0473 protein STH1993</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1993</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0473 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196120.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1993"/>
    <dbReference type="EnsemblBacteria" id="BAD40978">
        <property type="protein sequence ID" value="BAD40978"/>
        <property type="gene ID" value="STH1993"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1993"/>
    <dbReference type="eggNOG" id="COG3906">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146610_3_1_9"/>
    <dbReference type="OMA" id="VNYNGND"/>
    <dbReference type="OrthoDB" id="2057244at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01448">
        <property type="entry name" value="UPF0473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009711">
        <property type="entry name" value="UPF0473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06949">
        <property type="entry name" value="DUF1292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="11548" checksum="CBB2A1FF3BBFE4BA" modified="2004-10-11" version="1">MSHDHEHEHDDEIIVLTDEEGNEHEFSIVQVITVDGKDYAILLPIEEGEEAEEAVIMRIDEEDGEDVLVEIESEEEFDRVADAWEEMLDDEEFEEESEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY7</accession>
    <name>Q67MY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1971</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1971"/>
    <dbReference type="EnsemblBacteria" id="BAD40956">
        <property type="protein sequence ID" value="BAD40956"/>
        <property type="gene ID" value="STH1971"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1971"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_2_2_9"/>
    <dbReference type="OMA" id="LRWHPGY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="53"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="26921" checksum="8537BE44DEC430F5" modified="2004-10-11" version="1">MTAAQVALLGIVLLLWEVAADRYWVNPMLTSRPSQIWRTLVSMASSGSLWMHLGVTLRETLAGFAIGMALGLGVAIALWWWPALAAVLDPYLVVLNALPKIALGPIFYIWLGHTLSVYGMAVAISVVVTIVMLHAGFSEVEANQVLVMRTFGATRGQILRKVVIPSSLPTVMAALKVNIGLTLVGVIVGEFLSARAGLGYLIIYGGQVFQMQLVMTSVVILAVISALLYLAVTWAERLVSRLLHLPMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ2</accession>
    <name>Q67MZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196093.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1966"/>
    <dbReference type="EnsemblBacteria" id="BAD40951">
        <property type="protein sequence ID" value="BAD40951"/>
        <property type="gene ID" value="STH1966"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1966"/>
    <dbReference type="HOGENOM" id="CLU_1325804_0_0_9"/>
    <dbReference type="OrthoDB" id="909233at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="22568" checksum="AF9ED782A0B0C5EF" modified="2004-10-11" version="1">MVKWIAGLVALAVAVGAAIWVGYSRGHGGLPAVFGSLERSVAIRADYQVYTTLEELVEEASVIAVGMVEGVEGTRNLARDPDNPDLPHPSLEIIGYDYRFVVEEYLKGDGPDTILVTEARKFLSHGREGIPAEPPAPEMESGGRYILFLNRAADAEGRWIGVAEPWRFALRDAEVQVESMWEAAPRAFPSVGEGEFVDQIRTLVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N27</accession>
    <name>Q67N27_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1931"/>
    <dbReference type="EnsemblBacteria" id="BAD40916">
        <property type="protein sequence ID" value="BAD40916"/>
        <property type="gene ID" value="STH1931"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1931"/>
    <dbReference type="eggNOG" id="ENOG50332KJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1266350_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="218" mass="21441" checksum="842F73D85F2E175A" modified="2004-10-11" version="1">MPLEHLTTTSGIGAPKHRCTVACRTGTGESRAQRGAAEYGHNGVPTKRLLLGRGGLLVLLARTNTLVRLGILLALSVLGAYIKLGPSSIAFDAMAGFVAALLMGPAAGALICGLGHVAVAAVTGFPLTLPFHLASAAAMAGVGCLGGLAARRFGLVAGAAVLVVANGILAPALLALLPNPLGLGLFAALALPLTVAAGANAAVALLVVLGLRRAGVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA7</accession>
    <name>Q67NA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195979.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1851"/>
    <dbReference type="EnsemblBacteria" id="BAD40836">
        <property type="protein sequence ID" value="BAD40836"/>
        <property type="gene ID" value="STH1851"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1851"/>
    <dbReference type="eggNOG" id="COG1302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120389_2_0_9"/>
    <dbReference type="OMA" id="HTNEFGN"/>
    <dbReference type="OrthoDB" id="1961470at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="181" mass="19731" checksum="DABE5372D5B25210" modified="2004-10-11" version="1">MGIIDRVVLSIYTFSLAIISGLMVLLAIAPEWIPVHIWLQDLLLTGRGRVVLGLVGSAFFVVSVRLIVFAFSSQGGGRPVIYETSMGEVHISLGAVESLVKKTARSVKGVRDMKAVITHAEDGLHAHLTGTVSPEVSIPEVSEEIQSAVRQYVRRVVGVEMAEVRIDIENIATDSRTRRLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB3</accession>
    <name>EX7S_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exodeoxyribonuclease 7 small subunit</fullName>
            <ecNumber evidence="1">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Exodeoxyribonuclease VII small subunit</fullName>
            <shortName evidence="1">Exonuclease VII small subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">xseB</name>
        <name type="ordered locus">STH1845</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the XseB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195973.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NB3"/>
    <dbReference type="SMR" id="Q67NB3"/>
    <dbReference type="STRING" id="292459.STH1845"/>
    <dbReference type="EnsemblBacteria" id="BAD40830">
        <property type="protein sequence ID" value="BAD40830"/>
        <property type="gene ID" value="STH1845"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1845"/>
    <dbReference type="eggNOG" id="COG1722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145918_3_1_9"/>
    <dbReference type="OMA" id="PLNDYKG"/>
    <dbReference type="OrthoDB" id="2057189at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00337">
        <property type="entry name" value="Exonuc_7_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003761">
        <property type="entry name" value="Exonuc_VII_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34137">
        <property type="entry name" value="PTHR34137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02609">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006488">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01280">
        <property type="entry name" value="xseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000207024" description="Exodeoxyribonuclease 7 small subunit">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00337"/>
        </source>
    </evidence>
    <sequence length="74" mass="8421" checksum="B73BBD1742F823B1" modified="2004-10-11" version="1">MTQELSFEEAIQRLEQVVRELESGDLPLERGLELFQEGVALARHCTALLDRAEARIEQLLERDGGVETLPFEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI7</accession>
    <name>Q67NI7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1771"/>
    <dbReference type="EnsemblBacteria" id="BAD40756">
        <property type="protein sequence ID" value="BAD40756"/>
        <property type="gene ID" value="STH1771"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1771"/>
    <dbReference type="HOGENOM" id="CLU_2588425_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="80" mass="8638" checksum="F7CAAEDF92187EF7" modified="2004-10-11" version="1">MDCLTPSRICRSGRGGSGRDSQSKNGSPTKAGGSSRQPSGTTALRLRTEPPFRICLRFTVTSSTSCSLLLRPLYQPRHAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP4</accession>
    <name>SURE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">STH1714</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195842.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NP4"/>
    <dbReference type="SMR" id="Q67NP4"/>
    <dbReference type="STRING" id="292459.STH1714"/>
    <dbReference type="EnsemblBacteria" id="BAD40699">
        <property type="protein sequence ID" value="BAD40699"/>
        <property type="gene ID" value="STH1714"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1714"/>
    <dbReference type="eggNOG" id="COG0496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_9"/>
    <dbReference type="OMA" id="MHVEERI"/>
    <dbReference type="OrthoDB" id="1909278at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235654" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <sequence length="256" mass="27853" checksum="186882E40B26A795" modified="2004-10-11" version="1">MALVLLTNDDGIFAPGINALRARMEQIPGLEVWAVAPDRERSASGHAITTYRPLFPVRVEIPGAVAPCISVTGTPADSAKLAIEAILPRRPDLVISGINRGANLGTDIFYSGTVAAALEGPILGIPALAVSLDSMTSSDYSAAADFAAQLALKVLEEGLPEGTLLNVNVPALPREAIKGVRVTKVGRRIYRDQWVRRMHPRGQEYYWLAGELAEIHNDRESDVSAVEAGYISVTPVHLDLTRYDQMDRLRQWNLTF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS3</accession>
    <name>METXA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine O-acetyltransferase</fullName>
            <shortName evidence="1">HAT</shortName>
            <ecNumber evidence="1">2.3.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homoserine transacetylase</fullName>
            <shortName evidence="1">HTA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metXA</name>
        <name type="ordered locus">STH1685</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13701"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="EC" id="2.3.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195813.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NS3"/>
    <dbReference type="SMR" id="Q67NS3"/>
    <dbReference type="STRING" id="292459.STH1685"/>
    <dbReference type="ESTHER" id="symth-metx">
        <property type="family name" value="Homoserine_transacetylase"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q67NS3"/>
    <dbReference type="EnsemblBacteria" id="BAD40670">
        <property type="protein sequence ID" value="BAD40670"/>
        <property type="gene ID" value="STH1685"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1685"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_1_2_9"/>
    <dbReference type="OMA" id="TRFCVVS"/>
    <dbReference type="OrthoDB" id="536745at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="PTHR32268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155743" description="Homoserine O-acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="52"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <sequence length="383" mass="42255" checksum="2E2CBD4E3543E5F7" modified="2004-10-11" version="1">MNAAPVPILLTQRRYARVATPENPLVLESGQRLTDVTLCYEVFGRLNPAGDNAILVCHALTGDSHVAGRYRPDDPKPGWWDDAVGPGKALDTDRYCVICSNVLGGCQGSTGPSSVNPATGRPYGLDFPLVTVRDMVRAQARLLDLLGVRRLLAVIGGSLGAMQALEWAATYPDRMRGIIPIGGAGRFHPQGIAFNEVQRQAILNDPGFLGGQYYGTPGPVRGLATARMLGMITYRSDESMWTQFGRNPQGEANPLHQGFAVAYQVESYLHYQGRKLVERFDANSYLYLTRAMDLMDLGRGRGSYEEAHARIQARVLAVGIRSDLLFPTYLQRETVELVRASGGRAEYVEMDSPWGHDAFLLDFPLIEEPIRRFLQELEAEENA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS9</accession>
    <name>DER_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">der</name>
        <name type="synonym">engA</name>
        <name type="ordered locus">STH1679</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195807.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NS9"/>
    <dbReference type="SMR" id="Q67NS9"/>
    <dbReference type="STRING" id="292459.STH1679"/>
    <dbReference type="EnsemblBacteria" id="BAD40664">
        <property type="protein sequence ID" value="BAD40664"/>
        <property type="gene ID" value="STH1679"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1679"/>
    <dbReference type="eggNOG" id="COG1160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016077_6_2_9"/>
    <dbReference type="OMA" id="KFRFLEY"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000099170" description="GTPase Der">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 1">
        <location>
            <begin position="3"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 2">
        <location>
            <begin position="178"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="KH-like" evidence="1">
        <location>
            <begin position="354"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="231"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="296"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <sequence length="471" mass="52802" checksum="BFB814750782F483" modified="2004-10-11" version="1">MKPIVAIVGRPNVGKSTLFNRLTQSRHAIVEDQPGVTRDRLYADTEWNGRTLTLVDTGGIQLDKEGDTIEAHVTRQAELAIREADVIIFVVDVTDGVTAPDLEVADLLRRQRKPVIVAVNKVENLKREDEALEFWALGLEPLINVSAEHGLGTGDLLDAVVAALPDLSEPEPEEGGPVRVAVIGRPNVGKSSLVNAILGEERVIVSDVPGTTRDAIDVLVERGEDKFLLIDTAGMRRKARVEEAVERYSVMRALRAVERAQVVLIVIDAQDGVTEQDQRIAGYAHENGKACIVVVNKWDLIEKDDRTMAKMTEEVRMRLAFMDYAMIHFVSAKTRARVHRLLPLIKEAAANHARRISTRELNDLVREAVALNPPPSDKGRRLKIFYATQPHVSPPGFVFFVNDSELVHFSYQRYLENQLRQTYAFEGTPINLYFRTREKAKLGERPVRVRRVLAGGQTREIRRAARKRSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT0</accession>
    <name>Q67NT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1678"/>
    <dbReference type="EnsemblBacteria" id="BAD40663">
        <property type="protein sequence ID" value="BAD40663"/>
        <property type="gene ID" value="STH1678"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1678"/>
    <dbReference type="eggNOG" id="COG4089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1189424_0_0_9"/>
    <dbReference type="OMA" id="YIILSIT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="24202" checksum="0DB0A60ED50313BD" modified="2004-10-11" version="1">MDVESPPVTAGGRAHREGRPMMPVGPILILLLLVLAATGFLTGPLRRIGLSGRAALLLLAAMLLGSALELRLAPRLTLNVGSGLLPALLSLYLLRTMRRWWEPLAAVGGAANAAASLALISLYFPPGLPTELNLFYLDAQYLYAAVAGTLGSVIGHTRPASFAAAVWGSLAADVYHYLHYSGAGHGDIVHRMGGGGFHGTALVAGVLALTLSELLQVGAPERRAAAPPHLPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ7</accession>
    <name>Q67NZ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195740.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40596">
        <property type="protein sequence ID" value="BAD40596"/>
        <property type="gene ID" value="STH1611"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1611"/>
    <dbReference type="HOGENOM" id="CLU_2060231_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="119" mass="13040" checksum="E9FAEA295E3B2D62" modified="2004-10-11" version="1">MASLVHKALDAARFTGSSPKGLYWAFRHPNPDAPPQTYRLCRRDRNGWKLLLAASAATGEVLAFYVETPEDQQAVREALRWLGIRGEVLPGDPAVLRLATGQEFPSTLVGGEKPKKRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P17</accession>
    <name>Q67P17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH-quinone oxidoreductase subunit F</fullName>
            <ecNumber evidence="12">7.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nuoF1</name>
        <name evidence="14" type="ordered locus">STH1591</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195720.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1591"/>
    <dbReference type="EnsemblBacteria" id="BAD40576">
        <property type="protein sequence ID" value="BAD40576"/>
        <property type="gene ID" value="STH1591"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1591"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_0_1_9"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 12" id="KW-0288">FMN</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0874">Quinone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="NADH_4Fe-4S" evidence="13">
        <location>
            <begin position="330"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="425" mass="46461" checksum="0883EC7F7C241175" modified="2004-10-11" version="1">MIQFEPVLTKGIGKVDLTNIDVYEAQGGYQALRKALAMEPQAIVEEVKKANLRGRGGAGFPAGVKWSFLPNDGRPRYLTVNADESEPGTCNNRTLIYANPHQLIEGILITAYANRITQSFIYIRGEFKRGAEILMKAIEDARRKGYVGQNILGTGFSQEITVHRGASAYICGEESALLNSLEGRRGEPRVKPPFPAVVGLYGCPTIINNVETISNVPHIIKNGWEWYTKITANPDNPKSCGPKIFTVSGCVQRPGNYELPMGVKLRDVIEIAGGLRPGRKLKAVQPGGSSTPMLTPDHLDVPMDFESVAAAGSLLGTAAVIVYDDTVDLVDVALYWARFYSHESCGQCTPCREGTHWLERVFTRIKEGGGTEGDLKMIADMQTQMAGTTICVLSDAAVAFPASLLKLFPEELDRYINRVKEVRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P81</accession>
    <name>Q67P81_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1527</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1527"/>
    <dbReference type="EnsemblBacteria" id="BAD40512">
        <property type="protein sequence ID" value="BAD40512"/>
        <property type="gene ID" value="STH1527"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1527"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_1_2_9"/>
    <dbReference type="OMA" id="HVPKTYE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="27445" checksum="EDD0A3F649629FD4" modified="2004-10-11" version="1">MRLDRLLGHMGFGSRKELRALLKAGRVTVDGRPVTDPGTLVDPGTSSVAVDGEPVRFQRHFHVLLHKPAGVITATADPRQRTVMDVLPEDLRRRDLVPVGRLDKDTEGLLLLTTDGTLAHRLMAPRWHQPKRYLARVDRPLDPADQEAFAAGVPLADGYVTLPARLEILSPQEGIVTVREGKYHQVRRMFAARGKRVVYLKRLSMGPLSLGDLPPGAARPLTAAEVDALYRSASLPNPDDPDVPLAGPPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P97</accession>
    <name>Q67P97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Magnesium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MgtC/SapB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1511"/>
    <dbReference type="EnsemblBacteria" id="BAD40496">
        <property type="protein sequence ID" value="BAD40496"/>
        <property type="gene ID" value="STH1511"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1511"/>
    <dbReference type="eggNOG" id="COG1285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079292_0_1_9"/>
    <dbReference type="OMA" id="ARFINIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003416">
        <property type="entry name" value="MgtC/SapB/SrpB/YhiD_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33778">
        <property type="entry name" value="PTHR33778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02308">
        <property type="entry name" value="MgtC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01837">
        <property type="entry name" value="MGTCSAPBPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="240" mass="25412" checksum="627AE5759C5973B0" modified="2004-10-11" version="1">MLSREFIELNLYTFAHEFVDTLIPLLVAALAGGVVGLERESHNRPAGLRTHVLVCVGSALLMRVSLGMYDMVLATGFGNGDPGRIAAQVVSGIGFLGAGTIMREGATIRGLTTAASLWVVSAMGLAVGAGFYMQTFVAVILVMLTLKTLSEVENRFFAHGAYRSLVVQVTDSPGKLGVVASLCGRWGYTIRNIAMRSGPLPGTIEIRFTVKPDGRSSDMAGLIGDLMSSEGVISVTEEDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE2</accession>
    <name>RS16_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsP</name>
        <name type="ordered locus">STH1466</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195596.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PE2"/>
    <dbReference type="SMR" id="Q67PE2"/>
    <dbReference type="STRING" id="292459.STH1466"/>
    <dbReference type="EnsemblBacteria" id="BAD40451">
        <property type="protein sequence ID" value="BAD40451"/>
        <property type="gene ID" value="STH1466"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1466"/>
    <dbReference type="eggNOG" id="COG0228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100590_5_0_9"/>
    <dbReference type="OMA" id="GFYNPIA"/>
    <dbReference type="OrthoDB" id="1937072at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000243880" description="30S ribosomal protein S16">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <sequence length="88" mass="10056" checksum="ADEB58B9BE96DD37" modified="2004-10-11" version="1">MALKIRLKRMGMKKTPFYRLVVTEATSPRDGRFVEEIGYYDPTKNPVVLNIDEEKALNWLLKGATPTETTRALLSKAGVLKKFDEARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH6</accession>
    <name>Q67PH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1432"/>
    <dbReference type="EnsemblBacteria" id="BAD40417">
        <property type="protein sequence ID" value="BAD40417"/>
        <property type="gene ID" value="STH1432"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1432"/>
    <dbReference type="HOGENOM" id="CLU_169738_2_0_9"/>
    <dbReference type="OMA" id="INDYANM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="8755" checksum="05C988B96C8FDCBC" modified="2004-10-11" version="1">MFGTGTTGQTGNSSNQLVVSRARAAMEQLKQQTAAELGIHNYDQQYKGDLPSRVNGSVGGYMVKKMIAIAESQLAGGTTTGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT8</accession>
    <name>Q67PT8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40305">
        <property type="protein sequence ID" value="BAD40305"/>
        <property type="gene ID" value="STH1320"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1320"/>
    <dbReference type="HOGENOM" id="CLU_3405860_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="30" mass="3285" checksum="4E05AAB470967900" modified="2004-10-11" version="1">MANVIGAAVLSSVLQVIFSIVVLRRPMPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX9</accession>
    <name>Q67PX9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glucosamine--fructose-6-phosphate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1279</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1279"/>
    <dbReference type="EnsemblBacteria" id="BAD40264">
        <property type="protein sequence ID" value="BAD40264"/>
        <property type="gene ID" value="STH1279"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1279"/>
    <dbReference type="eggNOG" id="COG0449">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827960_0_0_9"/>
    <dbReference type="OMA" id="QSFYVMA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="3">
        <location>
            <begin position="63"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="3">
        <location>
            <begin position="228"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="388" mass="41135" checksum="5F5DAE4A254E3D3B" modified="2004-10-11" version="1">MRPGARNLPSGRCMGMTQAIPESIRQAFERTEHPFHMWEGLQSTPEALATILEPPVQAQIAAAAEALAGVDGVHLLGCGTSYFSGIAGTYAFHALTGIPGAAHNAFEFAAYPPGRLERSAVIAISHTGSTGVALDAVRLAAQRGAVTIGLTDYPDSALAGAVAFPILGGGGREKSLPKTKSYPASLLRHYLLAARVAENRGGAGGEWAHALFRAPELARTVMADSLPLVEEVVAERLPASQVFLIGNGPNVATALEGGLKLQESAQVPAHAWQLEEAMHGPWSIINPGDWVILLAMRGPGLAKAQGLAEALQHIGVNLWVITDEPDAVPGARRVTRLPAGIPELFTPLFAILPLYQFTYLTALALGKRPDCMRLADDRYLRARLALPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q54</accession>
    <name>CARB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbamoyl-phosphate synthase large chain</fullName>
            <ecNumber evidence="2">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">carB</name>
        <name type="ordered locus">STH1204</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarB family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195335.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q54"/>
    <dbReference type="SMR" id="Q67Q54"/>
    <dbReference type="STRING" id="292459.STH1204"/>
    <dbReference type="EnsemblBacteria" id="BAD40189">
        <property type="protein sequence ID" value="BAD40189"/>
        <property type="gene ID" value="STH1204"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1204"/>
    <dbReference type="eggNOG" id="COG0458">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_2_9"/>
    <dbReference type="OMA" id="IEPAGIH"/>
    <dbReference type="OrthoDB" id="48855at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_A">
        <property type="entry name" value="CPSase_L_chain_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_B">
        <property type="entry name" value="CPSase_L_chain_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_1000066390" description="Carbamoyl-phosphate synthase large chain">
        <location>
            <begin position="1"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp 1" evidence="2">
        <location>
            <begin position="133"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp 2" evidence="2">
        <location>
            <begin position="672"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="3">
        <location>
            <begin position="948"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Oligomerization domain">
        <location>
            <begin position="402"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate synthetic domain">
        <location>
            <begin position="547"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain">
        <location>
            <begin position="948"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="159"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="698"/>
            <end position="755"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="821"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="833"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="833"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="835"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01202"/>
        </source>
    </evidence>
    <sequence length="1084" mass="117750" checksum="1895C929A17EC31A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q64</accession>
    <name>Q67Q64_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1194"/>
    <dbReference type="EnsemblBacteria" id="BAD40179">
        <property type="protein sequence ID" value="BAD40179"/>
        <property type="gene ID" value="STH1194"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1194"/>
    <dbReference type="HOGENOM" id="CLU_021293_2_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13751">
        <property type="entry name" value="DDE_Tnp_1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF772" evidence="1">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="290"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="48149" checksum="343A3BB866FEB734" modified="2004-10-11" version="1">MELIAYLFGIRSDRRLVEEIRVNVAYRWFLGLGLTDPVPHFTTPGKNYSRRWKDSGLFEELFDHVVKQAIDAGYIDGRMIFTDSSHLKANANKRRIAKEGTKGVTLEDIARARERHLAARRAEREECAANDDTEDGLLAAVNADREAHGLKPLPERKEDPQPDLSVDEMTVSLTDPEAAMLRREGKPDGFHYLQHRTVDGRHGFILDVLVTSAAMTDAQVYPTCLSRVDRHGLKVEKVGVDAGYNTLEVLHLLSKRGIQAAVAHRRHPSPKELMGKWRFKYDASRDAYRCPAKQWLTYVTTNRDGYRVYRSDASVCASCPLLGQCTRSTTKQKVIHRHLYEHLREEAREFVKTDEGQRLAQRRRETVERSFADAKELHGLRYARYRGRKRVQHQCLVSALAQNLKKLALLESRRSSYALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC0</accession>
    <name>Q67QC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1138"/>
    <dbReference type="EnsemblBacteria" id="BAD40123">
        <property type="protein sequence ID" value="BAD40123"/>
        <property type="gene ID" value="STH1138"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1138"/>
    <dbReference type="HOGENOM" id="CLU_3405859_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="30" mass="3178" checksum="BC4BA5DEC5C07765" modified="2004-10-11" version="1">MAEGRAKPMLTICNAGGFARAFVRSFKGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG7</accession>
    <name>Q67QG7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">infC</name>
        <name evidence="8" type="ordered locus">STH1091</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the IF-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1091"/>
    <dbReference type="EnsemblBacteria" id="BAD40076">
        <property type="protein sequence ID" value="BAD40076"/>
        <property type="gene ID" value="STH1091"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1091"/>
    <dbReference type="eggNOG" id="COG0290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054919_3_2_9"/>
    <dbReference type="OMA" id="RNMIMFL"/>
    <dbReference type="OrthoDB" id="1760144at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00080">
        <property type="entry name" value="IF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="7">
        <location>
            <begin position="24"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="6">
        <location>
            <begin position="100"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="199" mass="23026" checksum="4B9598741E3BF161" modified="2004-10-11" version="1">MCFGTRLPVLPVRRCSCIKDQLRVNEMVRAREVRLIDENGEQLGVFSSREAFRIAQERGLDLVEVAPNAKPPVCKLMDYGRYKYEQAKREREARKKQKVITIKEVKMRPNIDDHDFAVRQRQAESFLRDGDKVKATIMFRGREVVHAQLGKEVLDRLLETVKDICVVERPPRLEGRNMIMILAPKANLEPKAAQQGQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH1</accession>
    <name>Q67QH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40072">
        <property type="protein sequence ID" value="BAD40072"/>
        <property type="gene ID" value="STH1087"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1087"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="1">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="329" mass="37434" checksum="28EBFD76D35F56C2" modified="2004-10-11" version="1">MRVNGLYMPSQVKVPAFILFHEQLGASAKVAWAGLQLRPRGQNHLSELCGLSRTAIKPSYRQLEQLSLLRPWTCSKGQHAYLPVDLLRSRCVIAQAKITYGALQLAPTFRDGQVEITVNQIARLTRCDPSTARRALRSLVDAGWVEVRRSRRTGPFQLILSNPQLDAQKKAIAEINRRLEKAPYRGEALMREWLTLLIDLDNFEDDASPGFLVNPYTGEEMQIDRFYPPNAGFEFNGAQHYGPTALYPSEEQARRQLGRDLIKQAICLRRGIHLAVVHAEDLSLQGMLRRIPDCLPRRRLDGQELVIAHLESRSRDYQQRTPLPMPVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH2</accession>
    <name>Q67QH2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">proC</name>
        <name evidence="5" type="ordered locus">STH1086</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1086"/>
    <dbReference type="EnsemblBacteria" id="BAD40071">
        <property type="protein sequence ID" value="BAD40071"/>
        <property type="gene ID" value="STH1086"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1086"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_0_1_9"/>
    <dbReference type="OMA" id="EQICTPK"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="3">
        <location>
            <begin position="9"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="4">
        <location>
            <begin position="167"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="29404" checksum="951EF3A7EF39299F" modified="2004-10-11" version="1">MERMLTGNVGFLGAGAIAEALIRGMLNAGVVQPEQVLVSNRSDRERLADLNRRYGVRTAGSKGYVVDVCQVVVLACKPKDVAGLLAEVGGRFRPGQIVLSLAAGIATSFIEERVADGVMVVRAMPNTSCQVGESATAVCLGRAATPEAMRLVTEMLASVGQVYTVPEEQMDAVTGLSGSGPAYVYYIVEAMIEAGEAVGLAPEVARALTLQTLKGAALTLATTGADPAVLREQVTSPGGTTAAGLQVLREAGFAQALISAIVRATERSRELGRMPAQAATEVGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM6</accession>
    <name>DPO4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase IV</fullName>
            <shortName evidence="1">Pol IV</shortName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dinB</name>
        <name type="ordered locus">STH1032</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195164.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QM6"/>
    <dbReference type="SMR" id="Q67QM6"/>
    <dbReference type="STRING" id="292459.STH1032"/>
    <dbReference type="PRIDE" id="Q67QM6"/>
    <dbReference type="EnsemblBacteria" id="BAD40017">
        <property type="protein sequence ID" value="BAD40017"/>
        <property type="gene ID" value="STH1032"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1032"/>
    <dbReference type="eggNOG" id="COG0389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012348_1_2_9"/>
    <dbReference type="OMA" id="KVRRYDF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01113">
        <property type="entry name" value="DNApol_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024728">
        <property type="entry name" value="PolY_HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11798">
        <property type="entry name" value="IMS_HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="SSF100879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0515">Mutator protein</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000084965" description="DNA polymerase IV">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="UmuC" evidence="1">
        <location>
            <begin position="7"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Substrate discrimination" evidence="1">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01113"/>
        </source>
    </evidence>
    <sequence length="361" mass="40616" checksum="8B54C783C8CE8A8B" modified="2004-10-11" version="1">MRTDLWVIHLDMDAFFASIEQRDNPRLRGKPVIVGGSPNSRGVVSTCSYEARRFGVRSAMPSREALRLCPQAVFIRPNHAKYAAVGRQVRAIMRRFTDVIEPLSIDEAFLDVSGQDAVRVARELKEAIRAELHLTGSAGVSYCKFLAKLASDMQKPDGLTVITWERAQELLPTLPVRKLWGVGPASEQALHALGIYTCGDLLAYDPDTLRKHFGKRADELILLARGIDPRPVVPYREAKSIGEENTFPVDQTDREYLSRLLERYADNLAEELRRQGLYARTVTVKIKWNVFVEGGPKGGDFLQITRSQTLPMPTNDARTIGRVAREIFARVEWEGRKVRLLGLSVHNLVKQGEQVQAYLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM9</accession>
    <name>Q67QM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195161.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1029"/>
    <dbReference type="EnsemblBacteria" id="BAD40014">
        <property type="protein sequence ID" value="BAD40014"/>
        <property type="gene ID" value="STH1029"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1029"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4991">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014322_1_0_9"/>
    <dbReference type="OrthoDB" id="1184688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08239">
        <property type="entry name" value="SH3_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00287">
        <property type="entry name" value="SH3b"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SSF50044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51781">
        <property type="entry name" value="SH3B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269330" evidence="2">
        <location>
            <begin position="22"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="SH3b" evidence="3">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="SH3b" evidence="3">
        <location>
            <begin position="89"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="777" mass="82963" checksum="791567C01587751B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN1</accession>
    <name>Q67QN1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195159.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1027"/>
    <dbReference type="EnsemblBacteria" id="BAD40012">
        <property type="protein sequence ID" value="BAD40012"/>
        <property type="gene ID" value="STH1027"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1027"/>
    <dbReference type="eggNOG" id="ENOG50333FQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186693_0_0_9"/>
    <dbReference type="OMA" id="HFSHEAD"/>
    <dbReference type="OrthoDB" id="2003548at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="8212" checksum="884A6D110CC578E7" modified="2004-10-11" version="1">MKMEFVEKELKSWGEIIITTSGGEKFEIHLGDTEFDYENRVIRLKSPNAHYVIEGDSVESITKHYGHPDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN5</accession>
    <name>Q67QN5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1023</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1023"/>
    <dbReference type="EnsemblBacteria" id="BAD40008">
        <property type="protein sequence ID" value="BAD40008"/>
        <property type="gene ID" value="STH1023"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1023"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_9"/>
    <dbReference type="OMA" id="YVKQIYR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="138"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="21978" checksum="FE8D8B03F0DC6F63" modified="2004-10-11" version="1">MDAVKRRILLVEDQTVVREGLRQLIESDADMEVVGEAAAVAEAVALAARLRPDVVLLDLKLADGSGLDAARQILQGSDPPAVLVLSTYADAALVRSALAMGASGYIPKTASFAEIASAVRTAAQGGVVIHPSLAGKLAGRGEETLREEEIAMLRMLAEGASYAEIGNRLYLSERTVRRHMNLLFDKMGVQNRTQAVAEAMRRGLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ9</accession>
    <name>Q67QQ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH999</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH999"/>
    <dbReference type="EnsemblBacteria" id="BAD39984">
        <property type="protein sequence ID" value="BAD39984"/>
        <property type="gene ID" value="STH999"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH999"/>
    <dbReference type="HOGENOM" id="CLU_2738573_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="8548" checksum="252D11CA4C18B039" modified="2004-10-11" version="1">MEWPHAYWEVPAMSEILPPEVRQKIDEWEIRQRYNPVDVEKVEGNLRMTKVRRTIPVTQHFHANHPGETSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU8</accession>
    <name>Q67QU8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 12">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH960</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 14">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195092.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH960"/>
    <dbReference type="EnsemblBacteria" id="BAD39945">
        <property type="protein sequence ID" value="BAD39945"/>
        <property type="gene ID" value="STH960"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH960"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_9"/>
    <dbReference type="OMA" id="QAGWMPT"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="15">
        <location>
            <begin position="5"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD39945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="43833" checksum="0239AD6DC68AD29F" modified="2004-10-11" version="1">MERRVVVTGMGLISPLGNSVRANWEAAVAGRSGIGPITRFDASRLPTRFAGEVRDFDPATVMDPKEVRRYDPFIHYALAAAAEAVEDAGLTFEGELAERTGVVFGSGMGGLEGIVENALILQERGPRRISPFFVPRCIINSAAGLISMKYGVHGPNYGTVSACASAGHAIADAMHIIRRGDADVMITGGSEATITELAFAGFSSAKALSTRNDDPQGASRPFDADRDGFVMSEGAGCLVLEELEHARRRGARIYAELAGAGMSGDAYHITAPHPEGRGAVLAMKGALRSARLNPEDVGYINAHATSTDLGDVQESLAIEQVFGDHARKLAVSGTKSMHGHLLGAAGAIEAILTVLSLYHQVLLPTINLHRPDPECRLDYVPHTAREVRGVEAALCNNFGFGGTNVSLAFRRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW8</accession>
    <name>Q67QW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">G/U mismatch-specific DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195072.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH940"/>
    <dbReference type="EnsemblBacteria" id="BAD39925">
        <property type="protein sequence ID" value="BAD39925"/>
        <property type="gene ID" value="STH940"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH940"/>
    <dbReference type="eggNOG" id="COG3663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042829_3_2_9"/>
    <dbReference type="OMA" id="FWPVLHL"/>
    <dbReference type="OrthoDB" id="2034230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000700">
        <property type="term" value="F:mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="PTHR12159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="1">
        <location>
            <begin position="6"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="177" mass="19720" checksum="BC975CFFAB87C091" modified="2004-10-11" version="1">MTRALPDYLAPGLRVVFVGFNPGETSAREGHYYAYPGNRFYWLLWQAGLTERRYAPHEDARLLEIGYGLTDLVGRSTRSSGDLSAAELRSGREELLAKLARYRPRVACYNGKGIYAALTGRRRVAYGLQPESVIPGVLDFVAASPSGRSREPLAEKLRLYRELRALIAEGDEGERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R95</accession>
    <name>Q67R95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39798">
        <property type="protein sequence ID" value="BAD39798"/>
        <property type="gene ID" value="STH813"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH813"/>
    <dbReference type="eggNOG" id="ENOG502ZEB5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_496879_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="548" mass="63278" checksum="8A5B9DFAD7AE8A42" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA2</accession>
    <name>Q67RA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH806"/>
    <dbReference type="EnsemblBacteria" id="BAD39791">
        <property type="protein sequence ID" value="BAD39791"/>
        <property type="gene ID" value="STH806"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH806"/>
    <dbReference type="HOGENOM" id="CLU_2994983_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="57" mass="6510" checksum="A7F0E5D161CF094F" modified="2004-10-11" version="1">MTEQPWIASFVVRLTGPPLRVLVRHVQSGQEMRCLRLEEALAFMEHCLQLSGKTEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM1</accession>
    <name>Q67RM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH687</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH687"/>
    <dbReference type="EnsemblBacteria" id="BAD39672">
        <property type="protein sequence ID" value="BAD39672"/>
        <property type="gene ID" value="STH687"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH687"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_16_2_9"/>
    <dbReference type="OMA" id="PENEIHN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="164" mass="18295" checksum="E15C4087CF9696E4" modified="2004-10-11" version="1">MSTCQSKAALAMPNYITMRRLNLIDTHLAMMLPYVASAFGTFLLRQTFRPVPRELEEAAAMDGCGPLRTLWHVFIPLARPTLVAYGLVSVSYHWNNFFWPMVVTNTPDTRPLTVGLALFAKAVEGGSDWALVTAGTVIVILPLLALFLIFQRQFISSFMHSGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN4</accession>
    <name>Q67RN4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH674</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH674"/>
    <dbReference type="EnsemblBacteria" id="BAD39659">
        <property type="protein sequence ID" value="BAD39659"/>
        <property type="gene ID" value="STH674"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH674"/>
    <dbReference type="eggNOG" id="COG3601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086673_0_1_9"/>
    <dbReference type="OMA" id="PYLIYEP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025720">
        <property type="entry name" value="RibU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38438">
        <property type="entry name" value="PTHR38438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037778">
        <property type="entry name" value="UCP037778_transp_RibU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="19281" checksum="E3137E00308ED8D2" modified="2004-10-11" version="1">MPMSAVPESAKVVRQVATSGVLSALAVVLALVIRFPLLPAAPYLIYEPSDVPLLLAAFRLSPLWVTGISAAVAVVMGLTGSGGLIGMTSRFVGSAALGLTAAVVYQRVAARGRGKVVSVVAGVVVYTLAEVALTLILGPLFFGDLQTALAMILPVVVPFNLIKGGLNGVAALLVDTGVSVWTGRSSRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ2</accession>
    <name>Q67RQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194789.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH656"/>
    <dbReference type="EnsemblBacteria" id="BAD39641">
        <property type="protein sequence ID" value="BAD39641"/>
        <property type="gene ID" value="STH656"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH656"/>
    <dbReference type="eggNOG" id="COG4279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392742_0_0_9"/>
    <dbReference type="OrthoDB" id="1906220at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="702" mass="76896" checksum="968EA845EB0B8DEA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS0</accession>
    <name>Q67RS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194771.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH638"/>
    <dbReference type="EnsemblBacteria" id="BAD39623">
        <property type="protein sequence ID" value="BAD39623"/>
        <property type="gene ID" value="STH638"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH638"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_7_2_9"/>
    <dbReference type="OMA" id="FMQPWDF"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="217" mass="24701" checksum="CFBFB181BD5FD393" modified="2004-10-11" version="1">MEFQELYDLVRTRRSIRRYRPDDVPDEMLQRAIELATWAPSGGNYQPWRFTVVKNRELIGQIADAVEARSRLMASWPEADRYREQADRWIRTSAFFRDAPACIAVQVGGYQSVADRLLAARGPQDPAAAEMMEWRRFGSSRIQTIGGAIMLLLLALHQQGLGACWMAGPLQAKAEIEKLLNVPEGWEFVALIPVGFPAEEREGAPRRPLDEVMEFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU9</accession>
    <name>Q67RU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194742.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH609"/>
    <dbReference type="ESTHER" id="symth-q67ru9">
        <property type="family name" value="BlEst2-lipase-like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39594">
        <property type="protein sequence ID" value="BAD39594"/>
        <property type="gene ID" value="STH609"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH609"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038340_0_0_9"/>
    <dbReference type="OMA" id="GNADSWY"/>
    <dbReference type="OrthoDB" id="929778at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269644" evidence="1">
        <location>
            <begin position="32"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="545" mass="56250" checksum="5D4CBF8A7A07F9E0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW4</accession>
    <name>Q67RW4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH594"/>
    <dbReference type="EnsemblBacteria" id="BAD39579">
        <property type="protein sequence ID" value="BAD39579"/>
        <property type="gene ID" value="STH594"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH594"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1199302_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021243">
        <property type="entry name" value="DUF2804"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868">
        <property type="entry name" value="PTHR35868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10974">
        <property type="entry name" value="DUF2804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="25269" checksum="1DFF02F7F97B050E" modified="2004-10-11" version="1">MNVERELLEPVPLCDDRGRLNPDAVGWSRRPLHRCNLRGHWLRKKRWNYWAITTETHLFSLTVTDLDYAGLGFAYFLDFGTGDFTEQTVLHLLGRGLTLPDDVEGEVRFDDEKMRLYMYDDGETVRLTAASPDFGGVPMQADFTVTRPPEQESVNVVIPWSRHRLSRRPCRPGGRCGSAIGASPLRASPAWTSGGASGPTGASGTGPPLQACRAATRSASTWAAAGPTGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW5</accession>
    <name>Q67RW5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH593</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH593"/>
    <dbReference type="EnsemblBacteria" id="BAD39578">
        <property type="protein sequence ID" value="BAD39578"/>
        <property type="gene ID" value="STH593"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH593"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703342_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="92"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="17833" checksum="5FB2F5C80E9F59CD" modified="2004-10-11" version="1">MGLLGAQRLENGYREFDESAVRRVQEISALLEVGFTVKEIQQIAHCLGDPVHGHQLCEVARNMYRDKLNEIRNRILQLEKISSRIAVRIAGKGMRPHERLGRRRQRVQRAGKEREPGASRACRDRDRRRHRHRARHGARLRTPGCEGAHHREAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY6</accession>
    <name>Q67RY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH572</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH572"/>
    <dbReference type="EnsemblBacteria" id="BAD39557">
        <property type="protein sequence ID" value="BAD39557"/>
        <property type="gene ID" value="STH572"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH572"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_5_0_9"/>
    <dbReference type="OMA" id="CLSCADM"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="77"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="31847" checksum="117737347FC43D62" modified="2004-10-11" version="1">MRRLNAPLVVGLVLTAGLVALLVLPEVVSLPDPHLGVHVQMEGRKLRLAPFAPGEFGYVLGSDMVGRDLLSRVIYGARYTLGLAAAVIVLRAVIALPAGLRAGWTGGRAARIVQAMAVGFGSIPALALVAMTLGGLRWLVPGQKGWLATYVAMLVLSGAPRMAEQVRRRAEEIVVPHVEAARALGATPWRILRRHVLPLMRADLAVMFSSEMAWVLLLMGQLAVFGIYVGGTVAVRGDGWIRYIEYLPEWGQMLGANRTAILGSPWIPFFPGLALGVSAAAFHLLAEGFRLQGLRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S08</accession>
    <name>Q67S08_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194684.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH550"/>
    <dbReference type="EnsemblBacteria" id="BAD39535">
        <property type="protein sequence ID" value="BAD39535"/>
        <property type="gene ID" value="STH550"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH550"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_8_0_9"/>
    <dbReference type="OMA" id="LEHHFTE"/>
    <dbReference type="OrthoDB" id="1434838at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022290">
        <property type="entry name" value="CHP03858_lucif-like_mOase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03858">
        <property type="entry name" value="LLM_2I7G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="347" mass="38089" checksum="3DF42B0BE5A63D84" modified="2004-10-11" version="1">MELGIYTFVETTPDPVTGARLSPEQRIRDLLEEIELADQVGLDVFGIGEHHRPEYVSSAPAVILAAAAARTKRIRLTSAVTVLSSDDPVRVFQQFATLDLISGGRAEIMVGRGSFIESFPLFGCDLNDYDALFAEKLDLLLRLRESEHVTWEGRFRPPLTGQGVYPRPVQQKIPVWVAVGGTQYSAARAGALGLPMALAIIGGLPERFRPLAELHRKAAVMNGHEPPALSINSHGFIADRSQDAADLAFPAFKAVMDQLGRERGWPPMTRAQFEASRSLRGADFVGSPQEVAEKILYQHELFGHQRFLLQLTVGSLPHDKVLRAIELFGTQVAPVVRKAVARKQGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S28</accession>
    <name>Q67S28_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39515.1"/>
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    <dbReference type="Proteomes" id="UP000000417">
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011624">
        <property type="entry name" value="Metal-dep_PHydrolase_7TM_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011621">
        <property type="entry name" value="Metal-dep_PHydrolase_7TM_intra"/>
    </dbReference>
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        <property type="entry name" value="7TM-7TMR_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7TMR-HDED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="HDc" evidence="3">
        <location>
            <begin position="489"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="754" mass="82208" checksum="E170034980792ADF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S65</accession>
    <name>Q67S65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ordered locus">STH493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH493"/>
    <dbReference type="EnsemblBacteria" id="BAD39478">
        <property type="protein sequence ID" value="BAD39478"/>
        <property type="gene ID" value="STH493"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH493"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490629_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0418">Kinase</keyword>
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    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
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        <location>
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            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
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            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="433"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="633" mass="69096" checksum="892AADCC36A2E9D7" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD5</accession>
    <name>Q67SD5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194557.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH423"/>
    <dbReference type="EnsemblBacteria" id="BAD39408">
        <property type="protein sequence ID" value="BAD39408"/>
        <property type="gene ID" value="STH423"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH423"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011543_3_2_9"/>
    <dbReference type="OMA" id="WYTTRER"/>
    <dbReference type="OrthoDB" id="970297at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023862">
        <property type="entry name" value="CHP03960_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03960">
        <property type="entry name" value="rSAM_fuse_unch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="254"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="636" mass="72079" checksum="2F714734521D388F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD9</accession>
    <name>Q67SD9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39404">
        <property type="protein sequence ID" value="BAD39404"/>
        <property type="gene ID" value="STH419"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH419"/>
    <dbReference type="HOGENOM" id="CLU_169738_3_0_9"/>
    <dbReference type="OrthoDB" id="2032801at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="65" mass="7117" checksum="BD6F662553090937" modified="2004-10-11" version="1">MARRRSLLSDETKMHFARLQGAGDRAQPGDYGQLTSREAGNMVKYAIQAAQQALAGQTPDPSRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF3</accession>
    <name>Q67SF3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF302 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH405"/>
    <dbReference type="EnsemblBacteria" id="BAD39390">
        <property type="protein sequence ID" value="BAD39390"/>
        <property type="gene ID" value="STH405"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH405"/>
    <dbReference type="eggNOG" id="COG3439">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126998_2_0_9"/>
    <dbReference type="OMA" id="LPCKIVV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005180">
        <property type="entry name" value="DUF302"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035923">
        <property type="entry name" value="TT1751-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016796">
        <property type="entry name" value="UCP021774"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03625">
        <property type="entry name" value="DUF302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021774">
        <property type="entry name" value="UCP021774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103247">
        <property type="entry name" value="SSF103247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF302" evidence="1">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="13819" checksum="63414E22057967A5" modified="2004-10-11" version="1">MEFAYEIPTGKGVEEAVAAVREALSERKFSVLWELNVNQTLESKGFDLGHEVRILEVCSAPKAKKALETNPAVAYFLPCKIVVKRVGGQTTIGVTRPAALMGLMGDARMDDLAAQVEAELTAAAEAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF6</accession>
    <name>Q67SF6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH402"/>
    <dbReference type="EnsemblBacteria" id="BAD39387">
        <property type="protein sequence ID" value="BAD39387"/>
        <property type="gene ID" value="STH402"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH402"/>
    <dbReference type="HOGENOM" id="CLU_1179723_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="24692" checksum="C2C29AC6AE669E3D" modified="2004-10-11" version="1">MRKVLAGVRGSIAVETAITLPLVLLLTVGGISVLLWLHHKTWMQALVAGTARERAADAAWTGYYKDIRDSLRASGSGLVLADVRLFSFHLPVDPPFVVAGACAAPAGRVPRIGTYGAPGAGSGVTAPTDGGGWLSPVQALRGQISRWLERLEGLAAEAEDHADAAVMLAEQAVWYRRVADNLAGGDPFRVRQAVDYLAGAAVEEVAALPCRTDGSGEVVLTAKAVIQGERTFGQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK0</accession>
    <name>CLPP1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease proteolytic subunit 1</fullName>
            <ecNumber evidence="1">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endopeptidase Clp 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpP1</name>
        <name type="ordered locus">STH358</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194492.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SK0"/>
    <dbReference type="SMR" id="Q67SK0"/>
    <dbReference type="STRING" id="292459.STH358"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="PRIDE" id="Q67SK0"/>
    <dbReference type="EnsemblBacteria" id="BAD39343">
        <property type="protein sequence ID" value="BAD39343"/>
        <property type="gene ID" value="STH358"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH358"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_2_9"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000179679" description="ATP-dependent Clp protease proteolytic subunit 1">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <sequence length="202" mass="22146" checksum="C232FAB363AF4342" modified="2004-10-11" version="1">MSYLIPYVIEQTNRGERSYDIYSRLLKDRIVFLGTPIDDQVANVVIAQLLFLESEDPDKPVNLYINSPGGSVSAGLGIYDAMQHIKSPVNTTVVGMAASMAALLLAAGTGKRYALPNARVMIHQPHLSGVGGQVTDIEITAREAVKTKQLMAEILAKHSGQSVERILADTERDRWMSAQEAVEYGLVDEVLHPREKASRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR7</accession>
    <name>Q67SR7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSII_BNR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH291"/>
    <dbReference type="EnsemblBacteria" id="BAD39276">
        <property type="protein sequence ID" value="BAD39276"/>
        <property type="gene ID" value="STH291"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH291"/>
    <dbReference type="eggNOG" id="COG4447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052334_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028203">
        <property type="entry name" value="PSII_CF48-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14870">
        <property type="entry name" value="PSII_BNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSII_BNR" evidence="2">
        <location>
            <begin position="58"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="36219" checksum="CF424AA59C8EDEE1" modified="2004-10-11" version="1">MGCSGATDGGSGEQNEDRNASAPAVPTAVAFLNESHGWVGAADGIWHTQDGGQNWTLQFPSQEPVIRLGFLDPQNGWALTGSAALLRTENGGEDWSAAETPGAALRAVDFVGPAHVVATDGNGLLVSRDGGKTWDRTSSPLPFSDLDFVSAEEGWAAGEGQIWHTRDGGTTWSAQLTLPEPDKWLGHTFVRFTSESSGWVLFCLGQGAGSQETYLLYSTTDAGRTWTPRLIGRWPWPFAEPAPEAPHGPGGHPVALGAWAETAWVAVYSPAGGHLEVARVTMGGAPPVVSDKIPVGGPAKPTAGLSFVDPKTGWLVVSDSQRTQGAILRTADGGQFWTAVLGNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR9</accession>
    <name>Q67SR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194424.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH289"/>
    <dbReference type="ESTHER" id="symth-q67sr9">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39274">
        <property type="protein sequence ID" value="BAD39274"/>
        <property type="gene ID" value="STH289"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH289"/>
    <dbReference type="eggNOG" id="COG0400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076826_0_0_9"/>
    <dbReference type="OMA" id="FEEFHQM"/>
    <dbReference type="OrthoDB" id="523836at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="307" mass="33748" checksum="6FBE1E56378B728F" modified="2004-10-11" version="1">MEVRGFRELQYEVYRLYGEGAYREALALIEREAARFAHRASDIYYWRACLACRAEGADAGLRWLQEAAERGLWYHERILRDPDLAPLRDDPRLKPLLAVFEERRAAAQATARPVLYTYSPEGEPKGLLLALHGNASNFEDSEERGQWLPALAAGWRVALAQSSQVWAPGKYFWSGRDQGLAEVSAHLGALNPPEATVVAGFSMGGALAVYAALCGSLPVSWFLAVAPAIRVESVLPLLETCPRDVQGYVVVGDQDFGYPAACRFAEAMQQAGLPCELEVRSGLAHDFPMDFSAGLTQRLEALCRAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T18</accession>
    <name>Q67T18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyprenyl synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH190"/>
    <dbReference type="EnsemblBacteria" id="BAD39175">
        <property type="protein sequence ID" value="BAD39175"/>
        <property type="gene ID" value="STH190"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH190"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_0_9"/>
    <dbReference type="OMA" id="GKQMRPM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="331" mass="36478" checksum="E081813694AAB763" modified="2004-10-11" version="1">MSSQPLAGNIRPLDVFSSIADDLRQVETAIEAALSTQETMLEEVSTHLLRSGGKRIRPALVILASRFPGVELKQVIDVAVAVELIHMATLVHDDVVDNADLRRGRPTVNALWNNQVSVLTGDYLFAKAFTLLADTGNNRVVRLMSEVVREMSQGELAQMASYFDVEQTEEDYYRRIARKTGYLIAEACRLGAVMAGVGEEQVQAVYDYGMGVGLSFQIADDLLDFFGDEETVGKPVCGDLKIGILTLPVIHGLAHAPRRAELRELILSRSIDDTAVARVKEILTESGSFDYARGKAREHIEKAVRALDHVPTLATRPALKTLAEFVINRKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-05-24" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T22</accession>
    <name>Y186_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein STH186</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH186</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T22"/>
    <dbReference type="SMR" id="Q67T22"/>
    <dbReference type="STRING" id="292459.STH186"/>
    <dbReference type="EnsemblBacteria" id="BAD39171">
        <property type="protein sequence ID" value="BAD39171"/>
        <property type="gene ID" value="STH186"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH186"/>
    <dbReference type="eggNOG" id="COG1660">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059558_0_0_9"/>
    <dbReference type="OMA" id="TVMSFGF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107778" description="Nucleotide-binding protein STH186">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <sequence length="302" mass="34302" checksum="AB094E58E2C43DBB" modified="2004-10-11" version="1">MMDVDRSIRLVIVTGMSGAGKTQALKYLEDLGFFCVDNLPPSLMPKLAELFGQTEGKVSRLALGIDIRGGRFFHEILGALRQIAEIGVAYQILFMDASDEVLVRRYKETRRRHPLAAQGRVLDGIQRERRLLQELRGLATFIIDTTHMTPADLRKELNRRFGQDRESPHFHVNVVSFGFKHGAVLDADLVFDVRFLPNPHYVPDLQPLTGEDPAVVEYVMKWNVTQQFYRRLTGLIGFLLPHYVAEGKSLLTIAIGCTGGKHRSVCLANRLAHWIRERGYSVSVEHRDMPRPADRSDEEEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T24</accession>
    <name>Q67T24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH184"/>
    <dbReference type="EnsemblBacteria" id="BAD39169">
        <property type="protein sequence ID" value="BAD39169"/>
        <property type="gene ID" value="STH184"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH184"/>
    <dbReference type="HOGENOM" id="CLU_2557036_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="9252" checksum="82C445B6EE17069C" modified="2004-10-11" version="1">MSERELRSLDLKEFLALVEADIRELDRRQLARHRLPSLPLRPVSCCGKRLGAFPPSARVRCPFCGTWIAPGEDPRPADPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T32</accession>
    <name>Q67T32_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194311.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH176"/>
    <dbReference type="EnsemblBacteria" id="BAD39161">
        <property type="protein sequence ID" value="BAD39161"/>
        <property type="gene ID" value="STH176"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH176"/>
    <dbReference type="eggNOG" id="COG3595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033702_0_0_9"/>
    <dbReference type="OMA" id="RSWDKDQ"/>
    <dbReference type="OrthoDB" id="731668at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025164">
        <property type="entry name" value="DUF4097"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016599">
        <property type="entry name" value="UCP012569"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13349">
        <property type="entry name" value="DUF4097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012569">
        <property type="entry name" value="UCP012569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4097" evidence="2">
        <location>
            <begin position="170"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="341" mass="37055" checksum="EB989635AA2CC4C4" modified="2004-10-11" version="1">MSQEERLLILQMVAEGKITASEGAELLRALEGHREEEPGVEAAPPRRERRSGTSATLASGLSSLFEEVVERVTGALTDFTGQRYEFPTEITGVFTADRVPLKVLTGNGHVELKGWDEPGYKAQILVKARGATEADARARAQDAYRLTATETGFQLETDRRFDWSDLAVHVTLFLPKGHTYHLETRTGNGNIRIEGIDLDDGTAKSGNGRITVLDVAADRLYLKSGNGSVDVEGDVADLEATTGNGSLRVVPLGRRSERIQLKTGNGPAAIDTGRLSRAVGVFIDAHTGMGSVSVNRSDLVYELDERNIGYKHVIAHTEGYDRAEQRVDIRVRTGLGSISVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T38</accession>
    <name>Q67T38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194305.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH170"/>
    <dbReference type="EnsemblBacteria" id="BAD39155">
        <property type="protein sequence ID" value="BAD39155"/>
        <property type="gene ID" value="STH170"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH170"/>
    <dbReference type="HOGENOM" id="CLU_2002766_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="124" mass="14570" checksum="2B68CD53779B2F6E" modified="2004-10-11" version="1">MRVDERKIRQYLRSEATRVVPPPDMWERIQREMDRDRLRQERRALRRRLFSVPRVAVAAFAVVMLGFLVAPYGLAARQMTLLPGRWFGIHRTWIAALTPTAWSEAGRPAVTDLRWSSQEITLLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD5</accession>
    <name>RF1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor 1</fullName>
            <shortName evidence="1">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prfA</name>
        <name type="ordered locus">STH73</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194208.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TD5"/>
    <dbReference type="SMR" id="Q67TD5"/>
    <dbReference type="STRING" id="292459.STH73"/>
    <dbReference type="EnsemblBacteria" id="BAD39058">
        <property type="protein sequence ID" value="BAD39058"/>
        <property type="gene ID" value="STH73"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH73"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_1_9"/>
    <dbReference type="OMA" id="ISDHRVG"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263371" description="Peptide chain release factor 1">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <sequence length="356" mass="40563" checksum="1E3093CB2F88F21D" modified="2004-10-11" version="1">MKQKLEGVVQRYEDLTYKLGDPSVINNPTEYRQVAREHNRLGPIVEKFRAYQKTERELDDVLEMLEGPLDPEERALFNQELRDLKEKLQTLSDELRILLLPRDPNDDKNVIMEIRAGAGGDEASLFAAELFRMYTRLAERHKWKTEVLSISENEAGGIKEVLFQINAEGAYSRLKYEGGVHRVQRVPVTESQGRIHTSTVTVAVMPEMEEVDIEIKPEDLKIEVQRAGGAGGQHVNKTESAVRMTHLPTGIVVYCADERSQMQNREKALRVLRARVADYYAQQAKSEEEQLRRAQVGTGERSEKIRTYNFPQDRVTDHRIGLTVHNIAAVMDGEIDHIIDALAQHEQAALLAREEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF7</accession>
    <name>Q67TF7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH51</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39036">
        <property type="protein sequence ID" value="BAD39036"/>
        <property type="gene ID" value="STH51"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH51"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_11_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="374" mass="42024" checksum="086E5DD24737F070" modified="2004-10-11" version="1">MQVDFAGDTVPIHDRETGEVWQAPIFVAVLPASNYTFAKAYPAQDLRSWIKAHCDALEFFGGVPEIIVPDNPKAGVTRACRYEPDLNPAYQEMAAHYGAAVIPARPRSPRDKAKVENAVLVVERWILAALRNHTFFSLHDANRAIREAVARLNDRPFQKLEGTRRSLYETLDKPALRPLPPQRYEFAEWRKAKVNLDYHVEVDRNYYSVPYPLVGETVDIRLTDGLVEILHRGKRVACHPRATGRGRSVTQIQHMPASHRRHREWTPSKLRRWAESVGPHTAQLVVTILEEKPHPEQGFRSCLGIIHLARKYGSDRLEAAAARACAARTFSYSSVKSILAHNLDQAPLPGPPDNQATPVHPNLRGPDYYAGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH5</accession>
    <name>Q67TH5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Similar to multidrug resistance protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH33</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194168.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH33"/>
    <dbReference type="EnsemblBacteria" id="BAD39018">
        <property type="protein sequence ID" value="BAD39018"/>
        <property type="gene ID" value="STH33"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH33"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_10_6_9"/>
    <dbReference type="OMA" id="FAVWGST"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="18"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="42604" checksum="603C7A850FED343B" modified="2004-10-11" version="1">MDSQVSTAASRSGSGWKVLTAVVFGVFMVILDSTVVNVAFKTLQEEYAANLNLTQWVLSVYTMALGIATPLSGFLGDRFGEKRVFIASIFLFGLSSLLCGLAPSLGLLILFRALQGAAGGVATPLGTSLLYKAFPPEDRGAAMGVFGIALVAAPALGPILGGWLVDLGHWRWIFYINVPIALVGTLLGSLLLPSSTGSRTARFDPLGLLFSSAGLGSLLYAASQASDLGWTAPEVVRFFLIGAVALLIFALVELFVAREPLLDLRLFGNRAFLVAILTGWVSVLALCGAEFLMPLYLQVLRGRTALEAGVLLLPAGERVHVHLLRLAGGTGPGCHAARMAGPDPAAEQGYSRGRCPFLGAQPPSFRHIANFEGPAAGVSAAGPPFLPRRAGGTVFAIVAERGVMQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK6</accession>
    <name>Q67TK6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">STH2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194137.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2"/>
    <dbReference type="EnsemblBacteria" id="BAD38987">
        <property type="protein sequence ID" value="BAD38987"/>
        <property type="gene ID" value="STH2"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038149_2_1_9"/>
    <dbReference type="OMA" id="YLIMPVR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="12">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="13">
        <location>
            <begin position="129"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="14">
        <location>
            <begin position="248"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD38987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="368" mass="40429" checksum="B0B02A50DF20107E" modified="2004-10-11" version="1">MKILISQESLNATLATVARAVSTKNTIPVLSGVYLSARGGELTIRATDMELAIEAVTPCEVISEGETVLPARYFADLIRRIPYGNIELSVDYHNHVATLRWGRSQYVIHGFAADQFPTLPDVDGASAFTVKQSLLRDLLRQTTFAAGHDESKPWLTGVLFKLRDDLLTGTATDGIRIAYAEAAAENAQGHSFSVIIPSRSLNEVTRLLTGDEDSQVNVAVTANQVFFDLGNTRVISRLLEGQYPDVMRLVPQHYPTEAVLPGAEFMEALERATLITKDGAVKISFQTDKVTITSNTPEVGQVYEELAVTSMAGDPLDIGFNARYLLDFLKVLEEKQFRFQCSGSRNPARLMPADSSQFLYVVLPLITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q3V817</accession>
    <name>Q3V817_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Flagellar biosynthetic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliR</name>
        <name evidence="8" type="ordered locus">STH2989</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FliR/MopE/SpaR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197104.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2989"/>
    <dbReference type="EnsemblBacteria" id="BAD41972">
        <property type="protein sequence ID" value="BAD41972"/>
        <property type="gene ID" value="STH2989"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2989"/>
    <dbReference type="eggNOG" id="COG1684">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063626_2_3_9"/>
    <dbReference type="OMA" id="APQFNIF"/>
    <dbReference type="OrthoDB" id="1942709at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002010">
        <property type="entry name" value="T3SS_IM_R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30065">
        <property type="entry name" value="PTHR30065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01311">
        <property type="entry name" value="Bac_export_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00953">
        <property type="entry name" value="TYPE3IMRPROT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="27455" checksum="C84CD331763FEECE" modified="2005-10-11" version="1">MEQLYAQLAALLLGASRAMGVFTAAPVFSTRFVPFQVRVALSFVLGFFLMPAIEVAPGRTEGVHLLTAALVEMVVGLVIGFLCLMVISVLQIAGALMDLEMGFALAGLFDPITGQGATVLGNLFQTTALVFFIALDGHHWLIRAFAQSYALVPAGGPVEGTASGLYVARLFGDLLALAVQLVLPFVAVMLLTMVAFAVLNRAVAQLNIFAIGLGTKTFVGLLLLVLLLPFYGRPLTALFEAGFREALRILELMRAVPGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-11-22" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J35</accession>
    <name>RSMG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="1">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmG</name>
        <name type="ordered locus">STH3334</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N7 position of a guanine in 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197445.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J35"/>
    <dbReference type="SMR" id="Q67J35"/>
    <dbReference type="STRING" id="292459.STH3334"/>
    <dbReference type="EnsemblBacteria" id="BAD42315">
        <property type="protein sequence ID" value="BAD42315"/>
        <property type="gene ID" value="STH3334"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3334"/>
    <dbReference type="eggNOG" id="COG0357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065341_0_0_9"/>
    <dbReference type="OMA" id="ICFPHLH"/>
    <dbReference type="OrthoDB" id="1590629at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003078">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000184350" description="Ribosomal RNA small subunit methyltransferase G">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="246" mass="26424" checksum="311520B2C86CE434" modified="2004-10-11" version="1">MDATAYRGLLEEGLASLGVEAEPGGVEAVLLHLDLVREWNERMNLTAITDPQEMVIKHALDAASGLAVAGVEPGQRVIDVGTGAGFPGVVWKCLRPGIDLTLLESLQKRCRFLEEVGQAVIGPLAGGEGYQVVWGRAEDVGRNPAHRERYDLVTARAVAELRVLAEYCLPLARVGGRFLAMKGPSVGEEILAAEAAVEKLGGRLEEVRELELPDGGGRRSLVLIRKERPTPKAYPRRAGVPAKSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J63</accession>
    <name>Q67J63_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YycH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197418.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3306"/>
    <dbReference type="EnsemblBacteria" id="BAD42287">
        <property type="protein sequence ID" value="BAD42287"/>
        <property type="gene ID" value="STH3306"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3306"/>
    <dbReference type="eggNOG" id="COG4863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_601190_0_0_9"/>
    <dbReference type="OrthoDB" id="451231at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009996">
        <property type="entry name" value="YycH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042274">
        <property type="entry name" value="YycH/YycI_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07435">
        <property type="entry name" value="YycH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="YycH" evidence="2">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="455" mass="49656" checksum="2E3539069F761B46" modified="2004-10-11" version="1">MTKPSGRAREVLLDLLLAALVLTSVVLTVRVWYPEPLFGDSDTTEPSLQQQPVPIVREMPEIFRPERIVVARADGGRAELHAGSPTYSTSWQRIRKALTGLDVRGGATLIDQVPRGDAGAPSLELFLPTALQVGQWADLLQWQAPFLRNGSMLVDRVIVTLGERPAVYLSGPLGFELYLADLPEDQRAALVAHVERLDPTLFGPYRELVLDGLQVTAAPGLMVPDVKAWPAAEVTVLMPDLWEEEARYFPDLSVVRQIDEQDARSLTDGRRLLRITGAGVLQYRTAEGSAPAASPELEQALEAAGQWVGSRGGWPQDVVLHRYERDAGVGRLAFEVHTGGRYPVQSLPGAMQVHVSAAARVVYFERTPTVANVTFEEALLPLVPPEDALAHALPAAPLLSSEPVRAMYLTYLLRPPAGAGEPWTAEPTWVIQAGSTEVYVNAVAREFPLPPKVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J71</accession>
    <name>Q67J71_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldehyde ferredoxin oxidoreductase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3298"/>
    <dbReference type="EnsemblBacteria" id="BAD42279">
        <property type="protein sequence ID" value="BAD42279"/>
        <property type="gene ID" value="STH3298"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3298"/>
    <dbReference type="eggNOG" id="COG2414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_705816_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFOR_N" evidence="1">
        <location>
            <begin position="5"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="391" mass="40423" checksum="EF16A5D61979FAA9" modified="2004-10-11" version="1">MDRAAERMLRVDLSSREVTVEPLPPLRGRKCIGPRGRAAACLLAGLDPASDPLGPGNRLAFTAGACAALPLPGASRLVVAGLSPVTGLYAEAEAGGAWAAALQRAGFAALVVEGRAERPVYLEVFEGGARLRDASRLWGLTTVETQAALRAGLADPAAQAVVIGPAGERKVPFACILHGAASAAGRAGLGAAMGAKGLKALVVRGGKVDGAPVAAGCAGCPRPCRSAVLGHKTRQAFAELGLVDEADLREANWLCNAYGLDTLATAAALRGAVRRRRRERREEVFRLIQFLARGELPCPPEAVRSADVRRQREGSPADWTADAARAAKILGICRWIVGPSGPLPVDQVLAGLGSAVGVPVTTADLAREEDRTEEAIAAFNRDAAAARISAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA5</accession>
    <name>Q67JA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyruvate ferredoxin oxidoreductase gamma-delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197376.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3264"/>
    <dbReference type="EnsemblBacteria" id="BAD42245">
        <property type="protein sequence ID" value="BAD42245"/>
        <property type="gene ID" value="STH3264"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3264"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060916_0_0_9"/>
    <dbReference type="OMA" id="DYQYCKG"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="255"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="293"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="37455" checksum="C24074DFEE0158E3" modified="2004-10-11" version="1">MAATPTQGTIRLPVKDEHGCFNIRLESIGGYGANIAGKMLAEAGVLYQGLNGSNFSSYGSEKKGSPVKSFIRFAEPDTPIRVSSPIEEPHIVCIFHEALIRTQPVVDGLRPGGVVIVNTRKRPEEIRQMLGVDDVTIGTFDATTIAVETGSRVNMAVLGAIARAAEFLDPEAIKALITDTFSKKYPHTVPGNLRCFDRGYAEIEFQSFGPSAEEGSARTRRSAAAYGYLNMPIGGVIPNPGNMAVKDLSASRQGFLPEFDREKCIDCAQCDMVCPDYCFVWEQGVDKRGRPAMVLKGINYQYCKGCLKCVEACPVDALREMREQDGYADAHTVRHDHLLVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA7</accession>
    <name>Q67JA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative chromosome replication initiation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197374.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3261"/>
    <dbReference type="EnsemblBacteria" id="BAD42243">
        <property type="protein sequence ID" value="BAD42243"/>
        <property type="gene ID" value="STH3261"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3261"/>
    <dbReference type="eggNOG" id="COG3935">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808728_0_0_9"/>
    <dbReference type="OrthoDB" id="1996763at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.630">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034829">
        <property type="entry name" value="DnaD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006343">
        <property type="entry name" value="DnaD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07261">
        <property type="entry name" value="DnaB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158499">
        <property type="entry name" value="SSF158499"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01446">
        <property type="entry name" value="DnaD_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaB_2" evidence="2">
        <location>
            <begin position="138"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="DnaB_2" evidence="2">
        <location>
            <begin position="228"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="39225" checksum="A3D69A77F5C5612A" modified="2004-10-11" version="1">MALGPDGPGEFGFLEAMMKRGFVSIPRMLFDYTLDLGLDYDRIGKIFTVLACVGGPGESPYTAYTVTRRNTPRDFDQVRTLVLQMEEDMITRCDHVTENEITFSFGPLFARLFAVWEEYWHEHQKETARKGPHPAVALAERMLGRSLSTRDVGSILEWVDEYGFTVEMVEAVIKEGFRQGVTRMSYLNSIAREWAEAGLETPEQAEAYIKEHEKTAARYRRATQALGIKRSLTAAEQAMIERWYEEWGFDDEVILQACEQAAGAKNPLQYTNKVLERWLQEGIRTRADLDRMVEQKRKAAAAGLDSGRSPRSGRRGSGQSNVILKREKKDDSYYEVVFKRFDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-21" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD0</accession>
    <name>PTH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="ordered locus">STH3238</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197351.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JD0"/>
    <dbReference type="SMR" id="Q67JD0"/>
    <dbReference type="STRING" id="292459.STH3238"/>
    <dbReference type="EnsemblBacteria" id="BAD42220">
        <property type="protein sequence ID" value="BAD42220"/>
        <property type="gene ID" value="STH3238"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3238"/>
    <dbReference type="eggNOG" id="COG0193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062456_4_1_9"/>
    <dbReference type="OMA" id="HVLSKFH"/>
    <dbReference type="OrthoDB" id="1676462at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000187836" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <sequence length="189" mass="20391" checksum="37A7C770F8119A52" modified="2004-10-11" version="1">MAKLIVGLGNPGPRYAATRHNAGWMLLDAFARKHGVAIEQRGFEGLYGELRWGAEAEKVILLKPLTYMNLSGRSVAQAARFYRIAPADILVLFDDLDLEPGRLRLRAKGSAGGHNGVKSVIAELGTAEFPRLRIGIGRPAPGWEVIDWVLAPFGPDDAAAVAAALPRAVEAVECFLTEGILAAMNRYNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE9</accession>
    <name>Q67JE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3219"/>
    <dbReference type="EnsemblBacteria" id="BAD42201">
        <property type="protein sequence ID" value="BAD42201"/>
        <property type="gene ID" value="STH3219"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3219"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_0_1_9"/>
    <dbReference type="OMA" id="QADAVWG"/>
    <dbReference type="OrthoDB" id="1567509at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026045">
        <property type="entry name" value="Ferric-bd"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002825">
        <property type="entry name" value="CfbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269228" evidence="3">
        <location>
            <begin position="30"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="358" mass="38797" checksum="72FDE1E7CB4F93FB" modified="2004-10-11" version="1">MWKRTLALTLAASLLVLAGCGSKQTPAPSTTPSTSNPPASSEAPKSNEPKKLVIYAGMMEDHVKGAVAEFEAQTGIKVEWVRMSSGETLTRIRAEKENPQASVWYGGPADAYVAAKEEGLLAQYVSPNAAKIPDQFKDPEGYWTGIYVGLLGFGYNTKMMEEKGLTPPESWEDLLKPEFKGQIALANPGSSGTSYTLLATIVQLMGEEKGLEYMTKLHEQVAQYPKSGTAPGQMAGRGEVLIGISFAHDIVKYAKEGMPLDVTFPKEGTGYEIGGIALINGAPDEEAAKIFIDWALTKEAQELGQKYGSYQSLTNVEATNPPEAFSLDEVTIIDYDLQWAGANRTQLVEKWSKAVNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG3</accession>
    <name>Q67JG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheX3</name>
        <name evidence="3" type="ordered locus">STH3205</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197318.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3205"/>
    <dbReference type="EnsemblBacteria" id="BAD42187">
        <property type="protein sequence ID" value="BAD42187"/>
        <property type="gene ID" value="STH3205"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3205"/>
    <dbReference type="eggNOG" id="COG1406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116290_1_1_9"/>
    <dbReference type="OMA" id="ICELANM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038756">
        <property type="entry name" value="CheX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028051">
        <property type="entry name" value="CheX-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39452">
        <property type="entry name" value="PTHR39452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13690">
        <property type="entry name" value="CheX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheX" evidence="2">
        <location>
            <begin position="43"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="16457" checksum="B1EA7BF6370DBD7D" modified="2004-10-11" version="1">MKLEVINAFLTAARHSLSQELQSEIKRTGLFVDPSENAIDEVAVYLSFVGQVRGTMLLGFDLTTARKIASAMVREPLAELTEMGLSALAELGNLIAGGTCIELEQLGFSADISPPTIMIGARSRISTLGIRRVVLPLATQHGHFNLHVAVDIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI4</accession>
    <name>COAX2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type III pantothenate kinase 2</fullName>
            <ecNumber evidence="1">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PanK-III 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantothenic acid kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaX2</name>
        <name type="ordered locus">STH3184</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197297.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JI4"/>
    <dbReference type="SMR" id="Q67JI4"/>
    <dbReference type="STRING" id="292459.STH3184"/>
    <dbReference type="EnsemblBacteria" id="BAD42166">
        <property type="protein sequence ID" value="BAD42166"/>
        <property type="gene ID" value="STH3184"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3184"/>
    <dbReference type="eggNOG" id="COG1521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066627_1_0_9"/>
    <dbReference type="OMA" id="HEPWLTL"/>
    <dbReference type="OrthoDB" id="2039419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00671">
        <property type="entry name" value="baf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000267592" description="Type III pantothenate kinase 2">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <sequence length="264" mass="28366" checksum="838D30F2A560E92D" modified="2004-10-11" version="1">MLLTIDVGNTFTVAGVFEGQNLLAEWTVTTDPNRTMDEYGMLFTQLMARAGFSSGQVTAVAIASSVPPLVPTLEWMCQKYFNLKPLVVGPGVRTGMMIRYDNPREVGADRIVGAVAAFDKYGGPLIVVDFSTAIILDAISARGEYLGGVIAPGIVVSADALFQFAAKLPRVELVRPPRALARNTVHAMQSGIIFGFASLVDDLVERMMAELDPEGKGCKVVATGEQAELLAGECATIQYCDPSLTLTGLRIIYERHQAHARGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI7</accession>
    <name>Q67JI7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3181"/>
    <dbReference type="EnsemblBacteria" id="BAD42163">
        <property type="protein sequence ID" value="BAD42163"/>
        <property type="gene ID" value="STH3181"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3181"/>
    <dbReference type="eggNOG" id="COG3039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_2_1_9"/>
    <dbReference type="OMA" id="FGTIKRQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13751">
        <property type="entry name" value="DDE_Tnp_1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF772" evidence="1">
        <location>
            <begin position="85"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="386"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="517" mass="59375" checksum="6C3E3B772AC03D86" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ8</accession>
    <name>Q67JJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-Aminoacylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197283.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3170"/>
    <dbReference type="EnsemblBacteria" id="BAD42152">
        <property type="protein sequence ID" value="BAD42152"/>
        <property type="gene ID" value="STH3170"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3170"/>
    <dbReference type="eggNOG" id="COG3653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016107_2_1_9"/>
    <dbReference type="OMA" id="HATMNFG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="50"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="334"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="482" mass="51821" checksum="7A1DB05B811A6D0B" modified="2004-10-11" version="1">MEATPIVYDLVIAGGTVVDGTGAPGFRGDVAVSGDRIVAVGDVGPYRAERVIDATGLVVAPGFINPHSHTHDDLLNPFIERDAKASLMQGITTEIGGVDGRSPVDIGAFLSRLEAEGTGVNYGTYLGQGSVRAHVMGWAEGPAGEAQLAEMKRLVREGMEDGALGLSTGLEYQPGSFAGTAELVELVKETAPYGGIYATHMRSEGDALVEAIEEALEIGRQAGVPVNLSHLKIVHYRNWGKEDEVVRLIEEARAAGQRVFADVYPYRAPDYAVNRPLAEWASALPPEHLLIVDGAPGELQGLTVAEAAALLGLPESEAARSLLARHPNTRVVALVSSERAMHRFYAADWSVVSTDGEAQPKLATAAEARAYAFHRRSYGTYPQLFEELVRKRGVLTLEQAVRKSTGQVADELGLKSRGYVKPGQYADLVLFDPATVADRTTWLSPQEYPEGIAYVLVNGHVVVDRGEWVGGRHGRIIRRGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM5</accession>
    <name>Q67JM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197256.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3143"/>
    <dbReference type="EnsemblBacteria" id="BAD42125">
        <property type="protein sequence ID" value="BAD42125"/>
        <property type="gene ID" value="STH3143"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3143"/>
    <dbReference type="HOGENOM" id="CLU_1325805_0_0_9"/>
    <dbReference type="OMA" id="ANLEPGP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="22782" checksum="031CD5E6569F161B" modified="2004-10-11" version="1">MRGYLITLLLIAFLVGSLFRASEVTGMRIFNMFATFGIFLFGVVGSLALTRLQQRSGQRGVEEALKALEPDWVITDWSAAPGERPDYLLVGPAGLLAVCVEHSPGQARSARARRRLAGAADRARQCADWVRSELSGWREAADLPVFPLVLLSRMRAPDDQEEDGVPVLNPESLAGFVANLEPGPALSRPLRYRITRRLREGRLPETG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP6</accession>
    <name>ISPF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <shortName evidence="1">MECDP-synthase</shortName>
            <shortName evidence="1">MECPP-synthase</shortName>
            <shortName evidence="1">MECPS</shortName>
            <ecNumber evidence="1">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispF</name>
        <name type="ordered locus">STH3122</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspF family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197235.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JP6"/>
    <dbReference type="SMR" id="Q67JP6"/>
    <dbReference type="STRING" id="292459.STH3122"/>
    <dbReference type="EnsemblBacteria" id="BAD42104">
        <property type="protein sequence ID" value="BAD42104"/>
        <property type="gene ID" value="STH3122"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3122"/>
    <dbReference type="eggNOG" id="COG0245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084630_2_0_9"/>
    <dbReference type="OMA" id="QHFGTGR"/>
    <dbReference type="OrthoDB" id="1716560at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000189506" description="2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00107"/>
        </source>
    </evidence>
    <sequence length="157" mass="16571" checksum="8C9730E6BF33DC2F" modified="2004-10-11" version="1">MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU0</accession>
    <name>EFG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor G</fullName>
            <shortName evidence="1">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA</name>
        <name type="ordered locus">STH3078</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197193.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU0"/>
    <dbReference type="SMR" id="Q67JU0"/>
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    <keyword id="KW-0342">GTP-binding</keyword>
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            <begin position="1"/>
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            <begin position="8"/>
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        <location>
            <begin position="284"/>
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        <location>
            <begin position="17"/>
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        <ligand>
            <name>GTP</name>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
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        <ligand>
            <name>GTP</name>
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            <name>GTP</name>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="694" mass="76872" checksum="126227ECEBA52B85" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY4</accession>
    <name>Q67JY4_SYMTH</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ordered locus">STH3033</name>
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        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
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            <taxon>Bacteria</taxon>
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            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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        <location>
            <begin position="68"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="218" mass="23989" checksum="537FFC5AF9DECF77" modified="2004-10-11" version="1">MVLVLLFVPFDLRLCGRADLAADDWESPLAGRAEWRLRLRWGLLPVTAGAQWSDGRLGQPEVRVLGFPVRGGNKGGRGHRPKTPGATKRKAGRKAGRTKPDPELLLTLAREAVQLPGRLWRSLGVRLTAEGSYGLPDPALTGLCEAIRWSAGLGRSLRLTPDFERPCLVGRGELTGRVYGFRLAAIAWHVARRPAVWNRLVGTIRFRPLRTILLRGGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-21" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ8</accession>
    <name>OTC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="2">OTCase</shortName>
            <ecNumber evidence="2">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argF</name>
        <name type="ordered locus">STH3019</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
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            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2"/>
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        <property type="protein sequence ID" value="BAD42002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197134.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JZ8"/>
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    <dbReference type="STRING" id="292459.STH3019"/>
    <dbReference type="EnsemblBacteria" id="BAD42002">
        <property type="protein sequence ID" value="BAD42002"/>
        <property type="gene ID" value="STH3019"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3019"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_9"/>
    <dbReference type="OMA" id="VYVKNWS"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
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        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113044" description="Ornithine carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <sequence length="310" mass="34016" checksum="8EC90838E7449014" modified="2004-10-11" version="1">MTTLKGRDFISLHDWSRDELEEVLELAMWQKARLKAGIRDEPLKGKQLGMYFAKPSTRTRLSFEVGIRQLGGDALFLSAHDLQLQRGESIADTARVMSRFLDGIMIRTFSHREVVELAEWATIPVINGLTDEEHPCQVVADLLTALERFGTLPGLKLAYVGDGNNVAHSLMDGGAKFGMHVVVASPEGYQPDPARVARAQVTAARYGGLVEVVTDPVLAVTGAQIVYTDVWASMGQEAEAEQRRKAFAGYQVTAELMRCAAPEAIFMHCLPAHRGEEVAAEVIDGPQSAVFDEAENRLHAQKAIMTLIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ9</accession>
    <name>Q67JZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197133.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3018"/>
    <dbReference type="EnsemblBacteria" id="BAD42001">
        <property type="protein sequence ID" value="BAD42001"/>
        <property type="gene ID" value="STH3018"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3018"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112782_0_0_9"/>
    <dbReference type="OMA" id="FTNVMIP"/>
    <dbReference type="OrthoDB" id="1515042at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="19075" checksum="FAF9791ABC6367A2" modified="2004-10-11" version="1">MFEYFKGPKGSWIWLVVRLYVGYQWLTAGWHKLVDGFDASGFLKGAIAKAVPASEGAKPVVQGWWAAFLEKFALPNVGLFNFLVPVGEFLVGLALILGFATIFAATMGMVMNFAFLLSGTISSNPNLLILEFILVALGGAYAGYIGVDYWFRPIWRSFLTRIFGKETATAKATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K07</accession>
    <name>Q67K07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acetyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197125.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3010"/>
    <dbReference type="EnsemblBacteria" id="BAD41993">
        <property type="protein sequence ID" value="BAD41993"/>
        <property type="gene ID" value="STH3010"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3010"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_3_9"/>
    <dbReference type="OMA" id="TRQVFFN"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="31"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="146"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="251"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="589" mass="64812" checksum="F96B63D561ACE070" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K17</accession>
    <name>Q67K17_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Flagellar-specific ATP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fliI</name>
        <name evidence="7" type="ordered locus">STH3000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197115.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3000"/>
    <dbReference type="EnsemblBacteria" id="BAD41983">
        <property type="protein sequence ID" value="BAD41983"/>
        <property type="gene ID" value="STH3000"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3000"/>
    <dbReference type="eggNOG" id="COG1157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_5_1_9"/>
    <dbReference type="OMA" id="MLMMDSV"/>
    <dbReference type="OrthoDB" id="875807at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030257">
        <property type="term" value="C:type III protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030254">
        <property type="term" value="P:protein secretion by the type III secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005714">
        <property type="entry name" value="ATPase_T3SS_FliI/YscN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040627">
        <property type="entry name" value="T3SS_ATPase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18269">
        <property type="entry name" value="T3SS_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01026">
        <property type="entry name" value="fliI_yscN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="154"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="436" mass="46566" checksum="25902991C043EDF1" modified="2004-10-11" version="1">MPWTKLRRAVEHVELLPVVGRVTGAAGLIIESAGPRAKMNELCWLQGDGRRVPAEVVGFREDRLLLMPLGETDGLRPGWDVIATGGPLQAPVGMGLLGRVIDGLGNPIDDKGPLMGCGFRPILGPAPDPLARQRIHRPLSLGVRALDALITVGMGQRIGIFAGSGVGKSTLLGMVARATAADCNVIALVGERGREVREFIEKDLGEEGLRRSVVVVATSEQPSLVRIRAALMATAIAEYFRDAHGLDVILMMDSVTRLAHAQREVGLAVGEPPATRGYTPSVFAMLPRVLERSGTGPAGSVTGVYTVLVDGDDMNEPVADAVRSILDGHVVLSRKLANAGHYPAIDPLQSVSRVMPDVTTPEHRKQAQRLRALLSAYQEAEDLIQIGAYQQGTNPLVDEALAKMDQIKRFLIQPADQPSTMEEALRGLAALCGEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K31</accession>
    <name>Q67K31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA polymerase sigma factor related to flagellar biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliA</name>
        <name evidence="8" type="ordered locus">STH2984</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2984"/>
    <dbReference type="EnsemblBacteria" id="BAD41967">
        <property type="protein sequence ID" value="BAD41967"/>
        <property type="gene ID" value="STH2984"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2984"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_1_9"/>
    <dbReference type="OMA" id="IKFETYA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012845">
        <property type="entry name" value="RNA_pol_sigma_FliA_WhiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02479">
        <property type="entry name" value="FliA_WhiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="6">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r3" evidence="5">
        <location>
            <begin position="102"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4" evidence="7">
        <location>
            <begin position="193"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="28599" checksum="01F619614706AE88" modified="2004-10-11" version="1">MQHLELWQRWRQSHDTAARDALVQHYLWLVRYVAGRLMVGMPDHVDQGDVEGHGCFGLLEAIARYDPDRGVRFETFAIPWIRGACLEGLRAMQWAPALRRRVRQLERVRDELMAVLGREPSAAELAEQMGISVEEAERRLQEAGTLAVLSLDEALAFDDGETTALADRVADEEAVDPELESQRAERREVLARAIASLTEQEQLVIALIYQEGLIAKEVSEILGLSQARISQVHSKAILRLRGKLSRMKRDLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K49</accession>
    <name>Q67K49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2966</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2966"/>
    <dbReference type="EnsemblBacteria" id="BAD41949">
        <property type="protein sequence ID" value="BAD41949"/>
        <property type="gene ID" value="STH2966"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2966"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="HTIALLP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="142"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="246"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="572" mass="60512" checksum="2B0FA44A332088CC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE1</accession>
    <name>ASSY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">STH2874</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196991.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KE1"/>
    <dbReference type="SMR" id="Q67KE1"/>
    <dbReference type="STRING" id="292459.STH2874"/>
    <dbReference type="PRIDE" id="Q67KE1"/>
    <dbReference type="EnsemblBacteria" id="BAD41857">
        <property type="protein sequence ID" value="BAD41857"/>
        <property type="gene ID" value="STH2874"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2874"/>
    <dbReference type="eggNOG" id="COG0137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_9"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="OrthoDB" id="357142at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148651" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <sequence length="401" mass="44594" checksum="F8B6C472B9981D64" modified="2004-10-11" version="1">MAKKKCVLAYSGGLDTSVAIHWIKENYDADVVALAVDVGANDKDLEFIRQKALSIGAVKSYVYDARKEFAYDFILPTLQANACYESKYYLSAALSRPLIAKLLVQIAEEEGADFVAHGCTGKGNDQVRFEVSVAALNPNLQVLAPVREWGWSREEEIDYAQKHGIPVPVGKENPFSIDVNLWGRSCEAGVLEDPWAEAPEEAFEWTVNPKDAPDEPEYVEIGFEQGVPVSLNGEALDLVTLIERLHAIAGRHGVGRIDHVENRLVGIKSREVYEMPAAAVLTLAHKELETITLPRELHHFKLGLEQKYAELVYNGLWFHPLKEALDAFIKKSQETVTGTVRVRLFKGSAFCVGRTSPYTLYSYDLATYDKADKFDHKAAKGFIDIFGLPTKVYAAVQKSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI4</accession>
    <name>Q67KI4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2829"/>
    <dbReference type="EnsemblBacteria" id="BAD41814">
        <property type="protein sequence ID" value="BAD41814"/>
        <property type="gene ID" value="STH2829"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2829"/>
    <dbReference type="eggNOG" id="COG1355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078734_0_0_9"/>
    <dbReference type="OMA" id="IETTSYF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="23821" checksum="A08DDF6DF0A164E7" modified="2004-10-11" version="1">MPHHDLAAELLSGFFLLLEEDPPEVIFLVGPNHEAAGPPVITGRRPWQTDFGLVEVDPGAVDALVGLGLAAVEEDVLAREHAMGTLMPYIKFHAPEARVVPLILHRGLSRADLERLAGALAAELGPGRILVASVDFSHYLTRPEAEAMDRETLATITAGDLDRLLQMGPEHLDSPGSVAVLLTAMAAAGADGPTVLGHTNSGAILRDDLIETTSYFTFVFTHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY2</accession>
    <name>Q67KY2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA polymerase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196801.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2679"/>
    <dbReference type="EnsemblBacteria" id="BAD41664">
        <property type="protein sequence ID" value="BAD41664"/>
        <property type="gene ID" value="STH2679"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2679"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_1_5_9"/>
    <dbReference type="OMA" id="WRVDLIP"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038969">
        <property type="entry name" value="FEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42646">
        <property type="entry name" value="PTHR42646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="53EXOc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="283" mass="30850" checksum="0880CF4EE17548EF" modified="2004-10-11" version="1">MLLVDGGLMFRTFFALPPMTDPKGRPVNAVYGFVSMLLRLLATLRPSHVAVAADSPVAENRRAVLYPPYKANRPECPPDLAPQFALLREVLAALNITVVGVRGYEADDLMGTMARMAEAEGMEVILLTGDRDAFQLLSGRTAVQYVKRLNQTITYDVARFVTEWGILPSQLADLKGLAGDASDNIPGIRGIGPKTAVRLLQQYVTLEAVLAHAHAQPGKLRQRLEEGREIALLSKRLATIDRHVPVSCTPGACALDLDLDTGAAVFEELHFRSLLPSLRRAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L37</accession>
    <name>Q67L37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41609">
        <property type="protein sequence ID" value="BAD41609"/>
        <property type="gene ID" value="STH2624"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2624"/>
    <dbReference type="HOGENOM" id="CLU_3104664_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="51" mass="5820" checksum="F2DBA980BD8BF77D" modified="2004-10-11" version="1">MNRLRVCAWCGSAIAEEQEEHRNLCPECSQVDELEAATPLYPVGERQPFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L85</accession>
    <name>Q67L85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41561">
        <property type="protein sequence ID" value="BAD41561"/>
        <property type="gene ID" value="STH2576"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2576"/>
    <dbReference type="HOGENOM" id="CLU_2002768_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="124" mass="12709" checksum="3396DDFFD2FBF545" modified="2004-10-11" version="1">MNSMSRVITGLGALVIVLGVIGAVWAARATPPGAIRVGLTTAAGLGGAFAGAVLLGLGAIIDRLDLLTGRGAQRAAEPAPPPVTPVTCPSCGERQDARADRPHHDCVKCGRPVIRDWVYLGRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L87</accession>
    <name>Q67L87_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Histidinol-phosphatase</fullName>
            <shortName evidence="7">HolPase</shortName>
            <ecNumber evidence="4 7">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2574</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + L-histidinol phosphate = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="4 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2574"/>
    <dbReference type="EnsemblBacteria" id="BAD41559">
        <property type="protein sequence ID" value="BAD41559"/>
        <property type="gene ID" value="STH2574"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2574"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054611_2_0_9"/>
    <dbReference type="OMA" id="KRIGHIT"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="PTHR21039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="8">
        <location>
            <begin position="7"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="271" mass="30444" checksum="8126F9BB0885FAF9" modified="2004-10-11" version="1">MLNRLADYHMHTVRCGHAVGAMEAYVETAIARGLGEIGFSDHVPMYWLPEERRDPTGAMAMEELEAYVSDVLRLRERYPEIPIRLGLEADFIPGHEEELVRLLEPYPWDYVIGSVHFLDDWNFDHPDLVHRYAEWDIDVLYDRFFALERAAAESGLFDILAHIDLIKKFGHRPRGDQSEAYAALADAIARSGVAIELSTAGLRKPVGEYYPAPALLKACCARGIPVVISSDAHAPEEVGWGFAEARALALEVGFTHTARFAGRRRQMVPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF4</accession>
    <name>Q67LF4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2507</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196630.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2507"/>
    <dbReference type="EnsemblBacteria" id="BAD41492">
        <property type="protein sequence ID" value="BAD41492"/>
        <property type="gene ID" value="STH2507"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2507"/>
    <dbReference type="eggNOG" id="COG3596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049716_0_0_9"/>
    <dbReference type="OMA" id="PFATMPV"/>
    <dbReference type="OrthoDB" id="546910at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021147">
        <property type="entry name" value="DUF697"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05128">
        <property type="entry name" value="DUF697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="46"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="405" mass="43585" checksum="CF1CB2A315E679DD" modified="2004-10-11" version="1">MDQIDRERLKEVLEFIRSHAPRPVLSSLEAELAVIEQYIVNARAPRIALVGRRGSGKSSLVNAIFGAPLAETGAVLSTTAAGTWYQYEGPNGSLEVLDTRGLGEGERPAGATEEDAVAENLKAVRDRCPDAILFLVKAVDVDAWIQNDLEQVAALRGAISRRHGFEPPVIGVLTQVDQLAPADVAEPPYDDPEKRANIRLALEKLAERMERLLDRPVRVFATSAYMRYRDGVRVVDRRWGIDELVSYLVETLPGEAQLTMGRVAGVKAAQTKIAGALIQSTASAAAAVAATPIPLADMPILTGMQVLMVTGIAYLAGRRLSREAALEFIGALGVNVGAGFALRELARGLSKWLLPGGGSLVSAGVAYWGTYGLGQAAVAYYIENQPIEAARKLLEQSRDRIVKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP6</accession>
    <name>Q67LP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose:sterol glucosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196538.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2415"/>
    <dbReference type="EnsemblBacteria" id="BAD41400">
        <property type="protein sequence ID" value="BAD41400"/>
        <property type="gene ID" value="STH2415"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2415"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_8_0_9"/>
    <dbReference type="OMA" id="GFPLFND"/>
    <dbReference type="OrthoDB" id="1485440at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033072">
        <property type="term" value="P:vancomycin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="1">
        <location>
            <begin position="3"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="43640" checksum="95DEE5351E71AFBD" modified="2004-10-11" version="1">MKFVILATGTRGDVQPFVALAQGLRAAGHEAVLAAPGSFESFVRSHGVGFFPMGGDYAALLESPEAQAALRGNPVKMMQVVRQTVFPMMGRMLEDAWAAAQGADALVYHPKALAGAHLVERLQVPCFIGAPVPVVVPTAAFPAPAFVSRDLGGFLNRLTYAAVRSGSRPFRGMINEWRRQVLGLGPRREGEYVQGGRLIPVLHAFSPHVVPRPADWPPEAIVMGYWFPRRDEPWEPPAALAAFLEEGPPPVYIGFGSVSGIDGDGSTRMAAEALARAGVRGVIATETGDAPVRLSDTVLAIPGAPHDWLFPRMAAVVHHGGAGTVAAGLAAGKPTLVCPATTDQPFWGRVVHRLGVGPAPIPRRRLTAERLAAAIGTLTTDEDIQRRAAALGEAIRAEDGVGRAVAEILRCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ2</accession>
    <name>Q67LQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41394">
        <property type="protein sequence ID" value="BAD41394"/>
        <property type="gene ID" value="STH2409"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2409"/>
    <dbReference type="HOGENOM" id="CLU_2290256_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="101" mass="11136" checksum="924A733972DA855F" modified="2004-10-11" version="1">MPSPVPGCQLNPGYLTREVSLFRSLQRSALRILATSRLNDRPVPSPVPGCQLNAGQLTREVSFFRSLQRSALRIFCHFTSERPPGAFPGPRMPAEAGILDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT0</accession>
    <name>Q67LT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196504.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2381"/>
    <dbReference type="EnsemblBacteria" id="BAD41366">
        <property type="protein sequence ID" value="BAD41366"/>
        <property type="gene ID" value="STH2381"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2381"/>
    <dbReference type="eggNOG" id="ENOG5033C7C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1593716_0_0_9"/>
    <dbReference type="OrthoDB" id="2093252at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="19009" checksum="1EC68994B8D3A95D" modified="2004-10-11" version="1">MVPEGTPQEFTLYRMQDGVRVTAVQVGDRVFIKPSPQHAAVKSRTAADQHYLTMADLQRQFYEPTIGVDVYDLADYEPGDTVLIRDRLVEVRYDAASDETTLVFSDEEGLHLDWAFRGNLTDRYAAGDTITLKFKVVEYAGEFEILDYMETLWTDGRAPALDNYLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT3</accession>
    <name>Q67LT3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2378"/>
    <dbReference type="EnsemblBacteria" id="BAD41363">
        <property type="protein sequence ID" value="BAD41363"/>
        <property type="gene ID" value="STH2378"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2378"/>
    <dbReference type="HOGENOM" id="CLU_069368_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269528" evidence="2">
        <location>
            <begin position="30"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="344" mass="38601" checksum="95E76A2DBBC359FA" modified="2004-10-11" version="1">MPNARRAATLLAILVLLTGCAGSQTDADATPVEKQASAADENDTPDRDAADRNIDPVNDREWALRPATRVDLNGDGLDELIIGELGRWSTEKVKVLRQDGSDLLKLDQWLPDTTQTRVDLVTMDGLPNPVLVIHVGDPDEWHSMEFTWMEGEELVSVWGWHPKHNVAFGEDYAIRPDGTVEITGDIAGFTIVRRYRIEPYADAYMPYVARKQDEVVIPGSYPTTAADLLTALFIARWYDLPDLVDQYLPDPDIRKAFMKEDFGEIRYRPIPVETGLLVYTDCGPQIEPADPDDNGLVEFLVSVASYEERLYWTGEAVISTERDGRLVVKDIRITDRGGFLPRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW7</accession>
    <name>Q67LW7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-malate dehydrogenase (decarboxylating)</fullName>
            <ecNumber evidence="5">1.1.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-malate + NAD(+) = CO2 + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18365"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.83" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2344"/>
    <dbReference type="EnsemblBacteria" id="BAD41329">
        <property type="protein sequence ID" value="BAD41329"/>
        <property type="gene ID" value="STH2344"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2344"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_9"/>
    <dbReference type="OMA" id="GTSMFEP"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046553">
        <property type="term" value="F:D-malate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011829">
        <property type="entry name" value="TTC_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02089">
        <property type="entry name" value="TTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="6">
        <location>
            <begin position="5"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="39434" checksum="84B6FFBFF8B12035" modified="2004-10-11" version="1">MQNVSVAVIPGDGIGNETVRAGRRVLDAAAELDGGIKFEYTEFEWGCAYYLRHGEMAPKGFLNTLANFDTILLGAVGYPGVPDHVSLWGLLLPIRRGFEQYVNLRPVRILRGVVSPLRGRNPGDVNFVCIRENTEGEYSNMGGRLHAGLPHEVVVQNTVFTRVGTERIIRYAYQLAANAPRKRLCGATKSNGINYTMPYWDEIFNEIGEREFPEVNRWLCHIDALAANFVLKPDEFDVVVASNLFGDILTDLGGAIMGSIGMAASANINPERRYPSMFEPVHGSAPDIAGRGIANPVGQIWSVSLMLEHLGRADLGKAVLDAVEDVLEEGRVRTPDLGGRNTTDEMTDAVIARLAQRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M92</accession>
    <name>Q67M92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2216"/>
    <dbReference type="EnsemblBacteria" id="BAD41201">
        <property type="protein sequence ID" value="BAD41201"/>
        <property type="gene ID" value="STH2216"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2216"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="297"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="552" mass="62113" checksum="99093864D499C77A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH7</accession>
    <name>Q67MH7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Penicillin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transpeptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2131"/>
    <dbReference type="EnsemblBacteria" id="BAD41116">
        <property type="protein sequence ID" value="BAD41116"/>
        <property type="gene ID" value="STH2131"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2131"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_1_2_9"/>
    <dbReference type="OMA" id="YQVVHGS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017790">
        <property type="entry name" value="Penicillin-binding_protein_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03423">
        <property type="entry name" value="pbp2_mrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBP_dimer" evidence="4">
        <location>
            <begin position="22"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="3">
        <location>
            <begin position="249"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="584" mass="63463" checksum="A99C417B24B2CB4A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM9</accession>
    <name>Q67MM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2079"/>
    <dbReference type="EnsemblBacteria" id="BAD41064">
        <property type="protein sequence ID" value="BAD41064"/>
        <property type="gene ID" value="STH2079"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2079"/>
    <dbReference type="HOGENOM" id="CLU_2541361_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="9412" checksum="1792ADB8E3781118" modified="2004-10-11" version="1">MEEGYVRGVSTFAGLVKVDHSENDRWRPESARRIFVAPEEARRLHPHWNRMLERALSAALALGPRRPPASDPQRTAKAPRSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP7</accession>
    <name>Q67MP7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ethanolamine utilization protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2061"/>
    <dbReference type="EnsemblBacteria" id="BAD41046">
        <property type="protein sequence ID" value="BAD41046"/>
        <property type="gene ID" value="STH2061"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2061"/>
    <dbReference type="eggNOG" id="COG4810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143326_0_0_9"/>
    <dbReference type="OMA" id="AMIAIND"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031469">
        <property type="term" value="C:bacterial microcompartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07046">
        <property type="entry name" value="BMC_PduU-EutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044870">
        <property type="entry name" value="BMC_CP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000249">
        <property type="entry name" value="BMC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037233">
        <property type="entry name" value="CcmK-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009307">
        <property type="entry name" value="EutS/PduU/CutR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40449">
        <property type="entry name" value="PTHR40449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00936">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012296">
        <property type="entry name" value="EutS_PduU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00877">
        <property type="entry name" value="BMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143414">
        <property type="entry name" value="SSF143414"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51931">
        <property type="entry name" value="BMC_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMC circularly permuted" evidence="2">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="112" mass="11815" checksum="441370D77DC41428" modified="2004-10-11" version="1">MEERQRIIQEYVPGKQVTLAHLIAHPTRELCERLGIEQAGAIGILTITPGEGAIIAGDVATKAAAVSIAFLDRFSGAVVIYGDVAGAETGLRAVNETLGEVLKFTPSLITRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS0</accession>
    <name>Q67MS0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SufBD_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196164.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2038"/>
    <dbReference type="EnsemblBacteria" id="BAD41023">
        <property type="protein sequence ID" value="BAD41023"/>
        <property type="gene ID" value="STH2038"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2038"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_0_1_9"/>
    <dbReference type="OMA" id="YYSAPKQ"/>
    <dbReference type="OrthoDB" id="635721at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="132"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="466" mass="52585" checksum="AE4F9E7B58B04717" modified="2004-10-11" version="1">MVSQPRDIVGEYKYGFRDPENYVFKSDKGLTREIVESISRFKNEPEWMREIRLKAFEVFHAKPLPTWGVDLSAIDFQNIHYFVRATERQGRSWDEVPEDIKRTFDRLGIPEAERKFLAGVSAQYESEVVYHNIRKDLEAQGVLFCDMDTAVREYPHIVKEYFGTVVPMTDNKFAALNTAVWSGGSFIYVPPGVTVEIPLQAYFRINTENMGQFERTLIIVDEGANVHYVEGCTAPIYSTDSLHAAVVEIIVKKGGRCRYTTIQNWSTNVYNLVTKRAVAYDDAVMEWVDGNIGSKVTMKYPAIYLMGPGARGDVISIAVASKGMIQDAGAKIYHFAPDTSSRITSKSISREGGNATYRGHILVEPQAERAKVKVECDALIFDDGSESHTIPYMDIKNSNVTIEHEATVSKVSEEQLFYLMSRGLSEEQALAMIVMGFIEPFAKELPMEYAVELNRLIEMEMEGSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV5</accession>
    <name>Q67MV5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma-W factor RsiW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2003</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily. Anti-sigma-W factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2003"/>
    <dbReference type="EnsemblBacteria" id="BAD40988">
        <property type="protein sequence ID" value="BAD40988"/>
        <property type="gene ID" value="STH2003"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2003"/>
    <dbReference type="HOGENOM" id="CLU_518496_0_0_9"/>
    <dbReference type="OMA" id="NYLMASQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="zf-HC2" evidence="4">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="535" mass="60273" checksum="814C5AFF10057205" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW1</accession>
    <name>Q67MW1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40982">
        <property type="protein sequence ID" value="BAD40982"/>
        <property type="gene ID" value="STH1997"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1997"/>
    <dbReference type="HOGENOM" id="CLU_2358633_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="11843" checksum="BD08752DD21E8045" modified="2004-10-11" version="1">MCWHCSGHDWDWDWDWNCDCQCPCRCRGDRRRRFDRKRRCDLHDDWDGDWDGHDRGRCRRSRRRRCDHRDDDPKSDLCSRPVVVHGHGGAKRFLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ3</accession>
    <name>Q67MZ3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196092.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1965"/>
    <dbReference type="EnsemblBacteria" id="BAD40950">
        <property type="protein sequence ID" value="BAD40950"/>
        <property type="gene ID" value="STH1965"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1965"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099771_0_0_9"/>
    <dbReference type="OMA" id="AIAEPHD"/>
    <dbReference type="OrthoDB" id="1435711at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="1">
        <location>
            <begin position="177"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="27326" checksum="9A3927620C4D2F93" modified="2004-10-11" version="1">MTAERFLRAYNRIDDYLRGLMDIDRTRYGFASVVDQAAKRYSVVRRYADDLKEFGALRNAIVHDKEFPARIIADPRPEVVEAIERLADIILDPPRVYPLFGREVRRLSWDDPIAKPLRWIREYHYTQFPVYRERRFAGLLTSRCVAQWLADSVHNGGVEFGGVTVGDVLRHDEYEGRRVVFVARDATWYDAVELLSPAKGKRRASLEAILITASGRDDEELLGIITPRDLLHPPAGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N17</accession>
    <name>Q67N17_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PTS system fructose/mannose-specific IID component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1941</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196068.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1941"/>
    <dbReference type="EnsemblBacteria" id="BAD40926">
        <property type="protein sequence ID" value="BAD40926"/>
        <property type="gene ID" value="STH1941"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1941"/>
    <dbReference type="eggNOG" id="COG3716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060742_1_0_9"/>
    <dbReference type="OMA" id="TLLCMWL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004704">
        <property type="entry name" value="PTS_IID_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03613">
        <property type="entry name" value="EIID-AGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51108">
        <property type="entry name" value="PTS_EIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="31121" checksum="B566673C11065B84" modified="2004-10-11" version="1">MANTQVDQSQVLPEYGPVTPADLRKAAWRHNLTLQWSWNYERMQALGFMWSILPILQRVSRTKEELIANMQRHLVFYNTNPAVGSPIIFGAACALEEKGQGEVADSLKVALMGPFAGIGDTIQAILLRPIVAVLAASLAMEGSWAGPLIMFIFGLCWFGLKFPQVTWGYKQGVSLVTTVAGGALEKVTEGATIMGLIVVGGFVPSIMAKVTTPIKFVRQVTVDGQLTEKVVELQPALDSILPYMIPLLIVGFAYWLLKARRQSPIKVLLWLTVLAFACSYLGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N49</accession>
    <name>Q67N49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Type I restriction-modification system specificity determinant protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1909</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1909"/>
    <dbReference type="REBASE" id="10271">
        <property type="entry name" value="S.StpORF1910P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40894">
        <property type="protein sequence ID" value="BAD40894"/>
        <property type="gene ID" value="STH1909"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1909"/>
    <dbReference type="eggNOG" id="COG0732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_10_2_9"/>
    <dbReference type="OMA" id="KRWFVDF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Methylase_S" evidence="4">
        <location>
            <begin position="14"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Methylase_S" evidence="4">
        <location>
            <begin position="194"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="44295" checksum="79CD50C3576A0435" modified="2004-10-11" version="1">MATLEYGKALRDYKTSTGSVPVYGTNGPIGWTNKPLCPFPTVIIGRKGAYRGVHLSPSPCWVIDTAFYISPKQPLDIRWAYYQLLTQDINGMDSGSAIPSTSREEFYRLPVKVPPLAVQKQIADVLGTLDSRIANVQSTNICLESIGQAIFKSWFVDFDPVRAKAEGRDPEGVDEDTAAWFPEEFQDSELGPIPKGWRVDTIDSVISCVGGSTPSTKEPAYWNPPEYHWVTPKDLSGQSTPVLLTTERMISEAGLKKISSGLLPEGTLLLSSRAPIGYLAITKIPTAINQGFIAMPPAGQLSPEYMLFWSHYNLDTIKQHANGSTFMEISKAAFRKIKLVVPPAQLVNRFTQIAQTVLERIAANERYRMQLVNLRDTLLPRLIAGKLRVPEAEEALKEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N63</accession>
    <name>Q67N63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196022.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1895"/>
    <dbReference type="EnsemblBacteria" id="BAD40880">
        <property type="protein sequence ID" value="BAD40880"/>
        <property type="gene ID" value="STH1895"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1895"/>
    <dbReference type="HOGENOM" id="CLU_1864131_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="137" mass="14469" checksum="4D6720BEB08829F5" modified="2004-10-11" version="1">MGKTVVGLFRHSGDADLAAAHLRDAFALDADRLNVIGEADLGAMSGARISDTDAWLLAAFADVGMEVGLDAGSDPGLDLAGSRAPVFRRWGDQVRRGRTLVVARADDPDEAVAIAGEMRRAGADRVDLIGEENWPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N68</accession>
    <name>Q67N68_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1890"/>
    <dbReference type="EnsemblBacteria" id="BAD40875">
        <property type="protein sequence ID" value="BAD40875"/>
        <property type="gene ID" value="STH1890"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1890"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_1_0_9"/>
    <dbReference type="OMA" id="CINVLPA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="2">
        <location>
            <begin position="42"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="20036" checksum="47AE1AE090E9E6E3" modified="2004-10-11" version="1">MRQSPTPSGGCAGRGGSAVPRHHRVGGSNMASFDSRTFRDTLGQFLTGVTVVTAAAPDGTPLGLTVNSFTSVSLDPPLVLFCIDKSAGCYEGVVNAQGYAVHILGGDQVDLANRFATKGTDRFAGLNYTRGFYGAPILPGAMALLECRTVQRVDGGDHTILIGQVERLNPGSRTRKPLGYFRGRYTAIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N71</accession>
    <name>Q67N71_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1887"/>
    <dbReference type="EnsemblBacteria" id="BAD40872">
        <property type="protein sequence ID" value="BAD40872"/>
        <property type="gene ID" value="STH1887"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1887"/>
    <dbReference type="eggNOG" id="ENOG5033H9C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155803_2_0_9"/>
    <dbReference type="OMA" id="RENTEWY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045443">
        <property type="entry name" value="DUF6504"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20114">
        <property type="entry name" value="DUF6504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="9608" checksum="72258424285CD611" modified="2004-10-11" version="1">MSRILNRPVDVTTGPDGRPAAFRFAGGRERVCAVLDTWMETGRWWEQEPELIAYRVETESGGVFELNFIPREQRWLLYKAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N74</accession>
    <name>Q67N74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1884</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1884"/>
    <dbReference type="EnsemblBacteria" id="BAD40869">
        <property type="protein sequence ID" value="BAD40869"/>
        <property type="gene ID" value="STH1884"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1884"/>
    <dbReference type="eggNOG" id="COG3012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099590_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="158" mass="18001" checksum="7C4C5C4070AD0B9A" modified="2004-10-11" version="1">MDLLQKVGRNDSCPCGSGRKYKTCCGLYHARIQYPDTVESLIRSRFTAYAIGDVDYLWRTTHPDNPARAGKTEAQFKRETLLYCSKVDFTRLVIHQTESEDENGISRGTLTAWFRIPGRPEDSFTERSEFKREDGRLLYLNGTELESPPAQQAQGADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE0</accession>
    <name>Q67NE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40803">
        <property type="protein sequence ID" value="BAD40803"/>
        <property type="gene ID" value="STH1818"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1818"/>
    <dbReference type="HOGENOM" id="CLU_563735_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269461" evidence="2">
        <location>
            <begin position="32"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="484" mass="53288" checksum="7BF07BCF575BC93B" modified="2004-10-11" version="1">MMISTPTQRWIAIGLLAVALTGCSFLSKAPSEEPVAPVQEPSDPTEPSEQPTPGSGDGEGPAGDERPPDDEPAAEEVTMLDCLRRFGLSELAPGVDELIRGLPDDGMWQCVARTARFDRNYRAWVAHEVLVSGVDHIDASLPGYRAFVRTTELHFAEPQTLPVPEGWTLQDALPSPSGRYLAARLADGGVGWWAADGRAQERYDVDGYDLVWHPEEDQLAFISDGRRLHLVRPAGPAVHQVYEAPAEAPVRFPYWALETWYAGYNESYPPGTLLVLADPEGEPEGLALRPDTGRWGRFPAKRIFDPVARAVRFPSWLTQPWSTLFGEYLHLVPDGGWLAYPNPGGSPYTFYQVPDGVEPVTLTWSPNARFFALVERRDGQLIAQVLRGSHDYTGRQYAVPLENAHIAVWDDGVTVFTVTGATVRAKNHLTGTEHAWQVEGEVKAIRLGGFRLYIVLEDRIIVVPFSEAASLSEAPRAEHPADAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF4</accession>
    <name>Q67NF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Metal-dependent peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH1804</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1804"/>
    <dbReference type="EnsemblBacteria" id="BAD40789">
        <property type="protein sequence ID" value="BAD40789"/>
        <property type="gene ID" value="STH1804"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1804"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086979_1_1_9"/>
    <dbReference type="OMA" id="HEIMHGL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="15">
        <location>
            <begin position="123"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD40789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="22956" checksum="ECFDEF0FB4FD1535" modified="2004-10-11" version="1">MALPSLQQIVMLIPGVVMGFAFHEFGHALVATWFGDDTPRLQGRLTLNPVAHLDPVGTLLLLLGGIGWAKPVMVNPARLRPRVLGDIAVSLAGVAMNFLLALVFVVLFVLAETGRLGWQHPVLSETLYLVVMVNVFLVGFNLLPIPPLDGFRVLAYAFPPGAHQLVQTLYRFGPLLLIFLFVTDLIDLSPIYTGIFNAVVWVASPLLRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP9</accession>
    <name>Q67NP9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195837.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1709"/>
    <dbReference type="EnsemblBacteria" id="BAD40694">
        <property type="protein sequence ID" value="BAD40694"/>
        <property type="gene ID" value="STH1709"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1709"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_813604_0_0_9"/>
    <dbReference type="OrthoDB" id="1123871at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="341" mass="36495" checksum="5F175B76DB3E181A" modified="2004-10-11" version="1">MRMSHSVRVPGGGAAHAFLLLVGLSLLVGAILRSAPGALPGSSVPASAGPEGEGRPEAEWAARLTPLTQTAAMDGFAVWSPDGREIAFMRDGQILLTTPQGRPVRVLTTQPGAWDAGPVWRPDGRALAFVRLSTRDGGAQVMLLPVPPGNAPDGKPVALTSEPGAIGYLAWDHSGDRLYYTTNDRIVRIAVPSGRKEEIFRAPEGWELISGGLAVAQDGRWLLFGGGPRTGTGVEYELYRLPLEGGEPERLTSGGGIMPGLHPGGRLVAYRNPRATTGIYVLDLATKEARRVLPDDARAMFFHPHFSPDGTKLLVSRLRLAPAQQRARGGFTSNIYVFEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV0</accession>
    <name>Q67NV0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1658</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195787.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1658"/>
    <dbReference type="EnsemblBacteria" id="BAD40643">
        <property type="protein sequence ID" value="BAD40643"/>
        <property type="gene ID" value="STH1658"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1658"/>
    <dbReference type="eggNOG" id="COG0651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_2_9"/>
    <dbReference type="OMA" id="YVAHHIT"/>
    <dbReference type="OrthoDB" id="1887859at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="4">
        <location>
            <begin position="128"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="494" mass="53726" checksum="B02731B889F70126" modified="2004-10-11" version="1">MSNLVAYPALLCFGTGIVQLLLHRRVALQRAVALLSSIAGIVLSVLLTYTIWTSGIQVQTAGGWPVPFGITLTADLFSGMMMILAAVVLFTSILYIFRDMNPDSERTFFYPVLFFMMAGVNLSFATGDIFNLYVAFEVMLISSYFLMALGSTGPQVREGLKYLLLNTVASSLFLFGVALLYGVTSSLNMADIAAKAASEPGNPYITLSGMLFLVVFAAKGALFPFYWWLPRSYYYVPNGIAPLFASMLTKVGVYGLIRVFTLIFIHDSGLTHSLLLVIGGLTMFLGVMGAISQYDFKAILSYHIISQIGYMIMGLGLYTVLGLAGAVFYIAHHIIVKSGLFLLSGATSRITGQTDFKHYSGLLASYPGLAYTFFATAISLAGVPPFSGFFAKFALLRAAVEKGAWGIAAVSLLVSFFTLFSMIKIFRKAYWGRPSGQRIQRTRRDYFAALTPAVILLALSVAMGLGGGYMMEFALATADQLMHPNIYVEAVLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV9</accession>
    <name>Q67NV9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1649"/>
    <dbReference type="EnsemblBacteria" id="BAD40634">
        <property type="protein sequence ID" value="BAD40634"/>
        <property type="gene ID" value="STH1649"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1649"/>
    <dbReference type="HOGENOM" id="CLU_1785915_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="145" mass="15307" checksum="3D30F15BF90414CD" modified="2004-10-11" version="1">MGGGGTHWISALPVRRALPFLLAAGPGLTAGNSSGWCASLRSAWPPRLPAPRRCSSGPRNRLDRLRGSAPPGGRAFPPERTRRRRKHRSRMTAGAVIPCARPGWITDCTGSSAHARTARPDDDTRRSAPVTRSGPAACTLSSGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY9</accession>
    <name>Q67NY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heme biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1619"/>
    <dbReference type="EnsemblBacteria" id="BAD40604">
        <property type="protein sequence ID" value="BAD40604"/>
        <property type="gene ID" value="STH1619"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1619"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_9"/>
    <dbReference type="OMA" id="GWGRQFF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="10"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="355" mass="38385" checksum="058F368382F5559A" modified="2004-10-11" version="1">MAVEEKPQSGARPRRVVWELTRACNLTCRHCRTVTCPAPTGKDLSTDQVVRVIEDIASVARPTFVFTGGEPLKRPDLFTIAAHASRHGAVVVLETNATMITAEAAAFLRTCGVAAVHVALDGATAETHDEFRGIPGAWAAAWRGIAHLKAVGLPFHLKFTIGVHNRDQVPAMLRLAEQEGAAGVHLCARMPAGCGIRLERDERLSPEEMEALLGWLSASFERTTVAVQATCAPQFHRVLRQQGGAAVVERQREQAHAVVGGGCSAATQGCFIAHDGQVQPCRHLPLPVGHVLQQRFQEIWEHALLLRQLRDRDLLGGKCGECGYRGACGGCRARAYEAYGFPLAQDPSCPYEPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA1</accession>
    <name>Q67PA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195637.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1507"/>
    <dbReference type="EnsemblBacteria" id="BAD40492">
        <property type="protein sequence ID" value="BAD40492"/>
        <property type="gene ID" value="STH1507"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1507"/>
    <dbReference type="eggNOG" id="COG1121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_9"/>
    <dbReference type="OMA" id="IAVLHDF"/>
    <dbReference type="OrthoDB" id="1721927at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="13"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="28757" checksum="28446AE8D5016A7B" modified="2004-10-11" version="1">MWEQCTAQGELPVVVRGLTVAYQRRPVLEDVNLSLPPGLLIGVIGPNGAGKSTLLKAILGLVPRVAGEVWIYGRPVAEARRAVGYVPQRAAVDWDFPTDVLDVVTMGRYGHLGLFRRPGARERAVAMEALEKVGMADLAHRQIGQLSGGQQQRVFLARALAQDARIYFMDEPFAGVDAATERAIIGVLHEMKARGRTVVVVHHDLQTVREYFDWLVFLNGHVLAAGPVERVFTPENLARTYGGRLGGLAQLPAAPQAAGRGWGGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB5</accession>
    <name>PYRH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">STH1493</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP. Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195623.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PB5"/>
    <dbReference type="SMR" id="Q67PB5"/>
    <dbReference type="STRING" id="292459.STH1493"/>
    <dbReference type="EnsemblBacteria" id="BAD40478">
        <property type="protein sequence ID" value="BAD40478"/>
        <property type="gene ID" value="STH1493"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1493"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033861_0_0_9"/>
    <dbReference type="OMA" id="PIIVFDM"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000054042" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in allosteric activation by GTP" evidence="1">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <sequence length="243" mass="26587" checksum="67F4998CC2A71327" modified="2004-10-11" version="1">MQTPKYRRVVLKLSGEALAGAKGFGLDPQVLTALARQIKAVHDMGVQVAIVVGAGNFWRGRLGEQMGMDRASADYMGLLATVMNALALQDAIEKLQVDTRVMTAVEMRQVAEPFIRRRAIRHLEKGRVVIFAAGTGNPYFTTDTAAALRAAEIEAEAILMGKQGVQGVYDSDPRINRHAVKYDEVTYQEVLAKNLQVMDATATALCMDNRIPIVVFDMMGPDHIVKVVLGEDVGQTFVRGNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE1</accession>
    <name>Q67PE1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding protein KhpA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">KH-domain protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">khpA</name>
        <name evidence="3" type="ordered locus">STH1467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">A probable RNA chaperone. Forms a complex with KhpB which binds to cellular RNA and controls its expression. Plays a role in peptidoglycan (PG) homeostasis and cell length regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms a complex with KhpB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KhpA RNA-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1467"/>
    <dbReference type="EnsemblBacteria" id="BAD40452">
        <property type="protein sequence ID" value="BAD40452"/>
        <property type="gene ID" value="STH1467"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1467"/>
    <dbReference type="eggNOG" id="COG1837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132074_3_0_9"/>
    <dbReference type="OMA" id="AVKMDKR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00088">
        <property type="entry name" value="UPF0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020627">
        <property type="entry name" value="KhpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34654">
        <property type="entry name" value="PTHR34654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8517" checksum="99BE3C967BD6B75A" modified="2004-10-11" version="1">MLQRLVEVLVTGLVEHPDQVKITAVETPRAVTFEVRVADSDMGKVIGRRGRVARAIRTVVKAAAVKDGRRVMVEFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM6</accession>
    <name>Q67PM6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1382"/>
    <dbReference type="EnsemblBacteria" id="BAD40367">
        <property type="protein sequence ID" value="BAD40367"/>
        <property type="gene ID" value="STH1382"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1382"/>
    <dbReference type="HOGENOM" id="CLU_2557039_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="8930" checksum="0A061713C8D5B6CF" modified="2004-10-11" version="1">MDRRTFPNRPAHHKVLKVRGDAGMDEAALIRAAQQRECLQRDYGTGYGTAGCRWTVDSMVVILVGPDLPSVVELAGSAVNHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR6</accession>
    <name>Q67PR6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Primosomal protein N'</fullName>
            <ecNumber evidence="10">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">priA</name>
        <name evidence="13" type="ordered locus">STH1342</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1342"/>
    <dbReference type="EnsemblBacteria" id="BAD40327">
        <property type="protein sequence ID" value="BAD40327"/>
        <property type="gene ID" value="STH1342"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1342"/>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013353_3_1_9"/>
    <dbReference type="OMA" id="RCHYCGY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="217"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="481"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="741" mass="81388" checksum="F4EBB63542CA0D77" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-01-04" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS0</accession>
    <name>Y1338_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative regulatory protein STH1338</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1338</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RemA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195469.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PS0"/>
    <dbReference type="SMR" id="Q67PS0"/>
    <dbReference type="STRING" id="292459.STH1338"/>
    <dbReference type="EnsemblBacteria" id="BAD40323">
        <property type="protein sequence ID" value="BAD40323"/>
        <property type="gene ID" value="STH1338"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1338"/>
    <dbReference type="eggNOG" id="COG2052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165326_0_0_9"/>
    <dbReference type="OMA" id="RDRNMLI"/>
    <dbReference type="OrthoDB" id="1786332at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01503">
        <property type="entry name" value="RemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007169">
        <property type="entry name" value="RemA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38449">
        <property type="entry name" value="PTHR38449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04025">
        <property type="entry name" value="DUF370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000050235" description="Putative regulatory protein STH1338">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01503"/>
        </source>
    </evidence>
    <sequence length="95" mass="10238" checksum="E4043A42AC08FF93" modified="2004-10-11" version="1">MGEIRLVNIGFGNIVSANRIVAIVSPESAPIKRIISEARERGTLIDATYGRRTRAVVITDSDHVVLSAVQPETVAHRLTARDASLAAAEEEEGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS7</accession>
    <name>Q67PS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1331"/>
    <dbReference type="EnsemblBacteria" id="BAD40316">
        <property type="protein sequence ID" value="BAD40316"/>
        <property type="gene ID" value="STH1331"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1331"/>
    <dbReference type="HOGENOM" id="CLU_2107750_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="13007" checksum="4AD8B2FE8F17448A" modified="2004-10-11" version="1">MRPGRHEIWLTFVRRTGTSEERVMPGRRDVRSVMTGQELAHAVAESIVLTVFGGEIQVVDQLPNVKLRLLDRRADLHLPGAPPLDQDGQVWLRVTVRRSEPGLLERLFGPRVHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT0</accession>
    <name>Q67PT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195459.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1328"/>
    <dbReference type="EnsemblBacteria" id="BAD40313">
        <property type="protein sequence ID" value="BAD40313"/>
        <property type="gene ID" value="STH1328"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1328"/>
    <dbReference type="HOGENOM" id="CLU_792082_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="350" mass="38431" checksum="BCB447E1B1A9A139" modified="2004-10-11" version="1">MEVRLDPRLLTLSAALTAGGLPPAGQDEHPLVTDTRRRLADHAAHPGVRRLCEAFAQHQLLGLAMHTVQLGPPPDFDDSCPDGVPPFVEAWFAAVDRTALAEALRSVWRDLRVGALLQEQAPRWEEVVRDLSSRLADAQIEPFQRLFWGRFPYEVVLVPLWNMNGAGLRGVGVATVRETYAVCPAQGDQPPGQVDLLVWAQHEASHPVLDDIQRQHGQVPGECAFVERDCPPAGGFARHYGDPTSRWVETLIHTSTCFYLEYLGRPEDAEAFAESQVEAGVTAIPVFREALRPWWEARRQGTAPGLELVLPQLPAWLRSALARRREADPAPGGPPGGSQDAGDPPPDMHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV3</accession>
    <name>Q67PV3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1998 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195436.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1305"/>
    <dbReference type="EnsemblBacteria" id="BAD40290">
        <property type="protein sequence ID" value="BAD40290"/>
        <property type="gene ID" value="STH1305"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1305"/>
    <dbReference type="eggNOG" id="ENOG502Z7NV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020062_0_0_9"/>
    <dbReference type="OMA" id="ACHACVL"/>
    <dbReference type="OrthoDB" id="878192at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="DUF1998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1998" evidence="1">
        <location>
            <begin position="469"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="605" mass="68336" checksum="1C55E5349EF4F692" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW7</accession>
    <name>Q67PW7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1291"/>
    <dbReference type="EnsemblBacteria" id="BAD40276">
        <property type="protein sequence ID" value="BAD40276"/>
        <property type="gene ID" value="STH1291"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1291"/>
    <dbReference type="eggNOG" id="COG1700">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1371596_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="199" mass="22951" checksum="C5180404EE84DC07" modified="2004-10-11" version="1">MHEVRVIHAKLELEGLFEFLDDVLTHVATQTTQHERLRFWLRESTRSAYDAPSHPAVPFLSKPPADTVVLLGYVRSPEHLRWIHEQRLYNMRTGGRRGSVLPGSRVLSAELVVLYGPHMRTAEMWRVAGTPLMLSEEEVRELHYPTPRGRYVCLPLEPLPSVELLQKMSSDHVRRVKERLSPTSYPGEPVAVTWFELLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q74</accession>
    <name>Q67Q74_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195315.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1184"/>
    <dbReference type="EnsemblBacteria" id="BAD40169">
        <property type="protein sequence ID" value="BAD40169"/>
        <property type="gene ID" value="STH1184"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1184"/>
    <dbReference type="eggNOG" id="COG3291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_437372_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017897">
        <property type="entry name" value="Thrombospondin_3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="SSF103647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="SSF49265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51234">
        <property type="entry name" value="TSP3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269309" description="Fibronectin type-III domain-containing protein" evidence="4">
        <location>
            <begin position="32"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="424"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="309"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="625" mass="66165" checksum="C8E71180288F481F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q77</accession>
    <name>Q67Q77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spore coat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195312.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1181"/>
    <dbReference type="EnsemblBacteria" id="BAD40166">
        <property type="protein sequence ID" value="BAD40166"/>
        <property type="gene ID" value="STH1181"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1181"/>
    <dbReference type="eggNOG" id="ENOG50328TJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1554517_0_0_9"/>
    <dbReference type="OrthoDB" id="1993599at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022121">
        <property type="entry name" value="Peptidase_M73_camelysin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023833">
        <property type="entry name" value="Signal_peptide_camelysin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12389">
        <property type="entry name" value="Peptidase_M73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04088">
        <property type="entry name" value="cognate_SipW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0946">Virion</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269489" evidence="1">
        <location>
            <begin position="25"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="17804" checksum="ABB8D9536DB460CF" modified="2004-10-11" version="1">MKRILLSLATVGALSALVTGATFALFTASTENTNNTFTAGTVRFGKDFNASCDLSADNIAPGDSGSCSYTITYVGSLEAFVGVEHSVSGDLFSGDNPMTYTVNGKSDSGTILLGKASNGDTVTADVQWSFPLAAGDEYEGKSGTISLTFKAVQVRNNEDASGNPISWNEDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q96</accession>
    <name>Q67Q96_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA polymerase sigma factor SigI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sigI</name>
        <name evidence="3" type="ordered locus">STH1162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Negatively regulated by the anti-sigma-I factor RsgI.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with RsgI.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. SigI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1162"/>
    <dbReference type="EnsemblBacteria" id="BAD40147">
        <property type="protein sequence ID" value="BAD40147"/>
        <property type="gene ID" value="STH1162"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1162"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082361_0_0_9"/>
    <dbReference type="OMA" id="KYVSCEN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02064">
        <property type="entry name" value="Sigma70_SigI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014244">
        <property type="entry name" value="RNA_pol_sigma-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30385:SF6">
        <property type="entry name" value="PTHR30385:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038953">
        <property type="entry name" value="SigI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02895">
        <property type="entry name" value="spore_sigI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="39"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Polymerase core binding" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="28404" checksum="9E4F41A954D405A2" modified="2004-10-11" version="1">MLLTFLLSLSDGTGERGPSPAERLLGPARAGDKAAREELIRAYTPLVLRVGSQVTGRYLRVGRDEEVSVGLIALNEAIDKFDPERGNAFIPFAEMVIRRRLVDFYRRNAGRVEVPLSEFTTEDDAGNPLQGLEMQEAIAAHQRQVEAEERRQEIARYARRLAEFGIRFSDLVEASPKHVDARERAIAIARMVAEDPALAGHLLSRRELPLKQLERRVEVSRKTLERQRKYIIAVALLLLEDFVYLRGYIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA7</accession>
    <name>Q67QA7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1151"/>
    <dbReference type="EnsemblBacteria" id="BAD40136">
        <property type="protein sequence ID" value="BAD40136"/>
        <property type="gene ID" value="STH1151"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1151"/>
    <dbReference type="eggNOG" id="COG1310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116765_1_0_9"/>
    <dbReference type="OMA" id="HEINAYC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070122">
        <property type="term" value="F:isopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="16630" checksum="E391DD76D44FD012" modified="2004-10-11" version="1">MSAQRRLVIGRRLLQQVLEHCLEEKPLEACGILAGKQGVVTSAFATDNARRSTVLYEVDPEHQGEALRRIAERGEELLGIYHSHPKAPAVPSQSDIAQAVHYPDAIRLIVSLDGPTEVGAFRIADGRVEEVPLQVLERLGGDFCDLRGWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA8</accession>
    <name>Q67QA8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195281.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1150"/>
    <dbReference type="EnsemblBacteria" id="BAD40135">
        <property type="protein sequence ID" value="BAD40135"/>
        <property type="gene ID" value="STH1150"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1150"/>
    <dbReference type="eggNOG" id="COG1310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116765_2_0_9"/>
    <dbReference type="OMA" id="ERPLEAC"/>
    <dbReference type="OrthoDB" id="1811367at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070122">
        <property type="term" value="F:isopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="19582" checksum="34A1F0666613376C" modified="2004-10-11" version="1">MLWWRRNRREGRPLAAPQRRILIPENIYRRMINHCYEERPLEACGLLVGAAGQVMAGYATDNQARSPVLYRVDDYQLLAAAEEAERRGREITAIYHSHVSSDPVPSQTDIRQATWPEAYYIIISLKGRRPRARAWRIVDGQVSEHRIVIARHYDGEWYDLRRAVQSAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM1</accession>
    <name>Q67QM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195169.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1037"/>
    <dbReference type="EnsemblBacteria" id="BAD40022">
        <property type="protein sequence ID" value="BAD40022"/>
        <property type="gene ID" value="STH1037"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1037"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_1_9"/>
    <dbReference type="OMA" id="FADIPVM"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="95"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="315" mass="34911" checksum="75730995257DEFEF" modified="2004-10-11" version="1">MGRYLVRRLLQMIPILFGVSIIVFVIIHSAPGDPYAYLFGPRIDPTLRARLMEEMGFNDPIPIQYVRWLTTTLKGNLGYSVRTQEPVVDMLARAIPRTLLLTGTAFLLGLALAIPIGVISATRQYSLIDYAATTFAFMGISLPSFFAALLAIYFFAVRLPIFPMNGIITPGGGGFWDVLHHLVLPAVTLGLRDMASYARFTRSAMLEVLRQDYVRTARAKGLAERVVVYKHAFRNGMIPVITLFGFSLPGLFGGAVIIEQVFTFPGMGLLTFEAVNNRDYSVLMATNMFFALLVILGNLIADILYAVADPRIRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT0</accession>
    <name>Q67QT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH978"/>
    <dbReference type="EnsemblBacteria" id="BAD39963">
        <property type="protein sequence ID" value="BAD39963"/>
        <property type="gene ID" value="STH978"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH978"/>
    <dbReference type="eggNOG" id="COG4932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_358592_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="SSF49452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269626" evidence="1">
        <location>
            <begin position="43"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="781" mass="81820" checksum="F28D343161B62AC0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX1</accession>
    <name>Q67QX1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH937"/>
    <dbReference type="EnsemblBacteria" id="BAD39922">
        <property type="protein sequence ID" value="BAD39922"/>
        <property type="gene ID" value="STH937"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH937"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_703071_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="329" mass="36777" checksum="D82917DB64B7060F" modified="2004-10-11" version="1">MSRGRPAPPRGGCRTGREGGDRMRRSRARMLGRYAFFLERFRSRYDLAEAYYRRALARDPEDAQTRRHYAIFLETVRGRYDEADAQYRAALRLAPNDPALLGDYADFLEHAVQDLDGAERYYRRALEADPLHPGNLTNYATFLTEVRGEHGRAEALYQRALEVAPLHRNALFKYALFLTDVKGAYDDAAELYRVALEAYPGNGAIMANLAGVLLLGGQAGEGRRMLSACLRHPALQRPTADAAEVRFYQVVYGDPEERPAALRALRRLLEAGVRSPGFQLGPHVAAARGQGHPWAHWLGSLAAVLTGEAEPAVLNPWEDWAEARPDPVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY2</accession>
    <name>Q67QY2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-cys peroxiredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH926</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195058.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH926"/>
    <dbReference type="EnsemblBacteria" id="BAD39911">
        <property type="protein sequence ID" value="BAD39911"/>
        <property type="gene ID" value="STH926"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH926"/>
    <dbReference type="eggNOG" id="COG0450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_21_3_9"/>
    <dbReference type="OMA" id="WWPKDFT"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="3">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="19892" checksum="BE51922EF560E950" modified="2004-10-11" version="1">MRLVGKPAPDFTMLSTKDLDKLDQKVSLSDYKGKWLILFFYPADFTFVCPTEITAFNDRVQEFQDANCEILGVSVDSVYSHRAWIKTPKEEGGLGPVNYPLASDITKEVSRAYGVLIEEEGVALRGLFIIDPDGIVRYQVVHDNNIGRNVDEVLRVLEALQAGGLCAANWKPGDALLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY6</accession>
    <name>Q67QY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39907">
        <property type="protein sequence ID" value="BAD39907"/>
        <property type="gene ID" value="STH922"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH922"/>
    <dbReference type="HOGENOM" id="CLU_2526298_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="84" mass="9131" checksum="3ABF80C795FEEF23" modified="2004-10-11" version="1">MSSHTLHCMGGGGDVFNELQLKLLNGRTISIHVEPGFDVDDLEYLPGLRAVSLGNLTVLYEGLVYDVRPRRTLAVHGAAPSLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R62</accession>
    <name>Q67R62_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma-W factor RsiW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH846</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily. Anti-sigma-W factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH846"/>
    <dbReference type="EnsemblBacteria" id="BAD39831">
        <property type="protein sequence ID" value="BAD39831"/>
        <property type="gene ID" value="STH846"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH846"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688724_0_0_9"/>
    <dbReference type="OMA" id="ITEQYYD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025645">
        <property type="entry name" value="DUF4349"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14257">
        <property type="entry name" value="DUF4349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-HC2" evidence="5">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="320"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="42287" checksum="325904F6DB281491" modified="2004-10-11" version="1">MNCDGVRPLLSGYIDQELSAGELLRVEQHLRRCHACAAEVDALRQTVALVASLDEVEVPATFHAQLRQRLMSEDPPIARVHTAGRGRNRLHSFQRWAIPAAAAAAFVIGAAGLNRFVPPAAQVEPPPGGAVVAAPGSHVDTAEVPGTTNPPGTDAQEQPHHPVDGPDANPPSGTGGRVEHPEGQLTEQLDPGSAGVVQTTPPPETGDANPAANPATTPAPLGPTRPTGGVTTASTSGDTAGQVELSPQPHFSATAEMTVASPAAEAAQLRDRFDRWRVQENGRVGTVELQIFVPAGEFREAVALVNSELAGYGVALKVQEKDLAGQIAETEDRIATLEEAREALAARLATETSQDRLEEGARELTKIQQQLETERANQQNLREAVENGVIVLTFKPEPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R80</accession>
    <name>Q67R80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH828</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH828"/>
    <dbReference type="EnsemblBacteria" id="BAD39813">
        <property type="protein sequence ID" value="BAD39813"/>
        <property type="gene ID" value="STH828"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH828"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_2_9"/>
    <dbReference type="OMA" id="IHEWPYL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="6">
        <location>
            <begin position="56"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="5">
        <location>
            <begin position="202"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="44195" checksum="4379C98CEDCA8F1B" modified="2004-10-11" version="1">MVHGLCLLLIETSLAGAGTGGQPGGPGRSVRRPGRSNAGHPSDKEAGHVRESMIRRFEAVRQFLRERGMAAGLVTGPISMRYLTGWSNPSKRFAGLVIPADGEPALLVPALEVEEVRSASALRLVAWQDGEDPFAALSGMLRAAGAGEGRIALEQADLPVGQLRRIAGALGLAPSVLLDAAADLSPALSAQRESKDEDEIALLQQAADMLNPALDAALAAIRPGVTEREIARVLEEAMLAAGADGVAFETHVLFGPASALPHGSTGARTLEPGHVVLMDFGAQLRGYRSDITRTVCCGAWPDELARVYDVVLAANQAAIAAVKPGVPLGDVDRAARQVIEEAGYGAYFIHRTGHGLGLEIHEEPYVVAGNEKVLRPGHVITIEPGVYLPGVGGVRIEDDVVVTEDGCRVLTSWTKERLSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R81</accession>
    <name>Q67R81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH827"/>
    <dbReference type="EnsemblBacteria" id="BAD39812">
        <property type="protein sequence ID" value="BAD39812"/>
        <property type="gene ID" value="STH827"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH827"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_0_2_9"/>
    <dbReference type="OMA" id="REWHRRG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27812" checksum="0736468EDFB5A051" modified="2004-10-11" version="1">MDGTLLRSDQTVSPRNQAAVAACLDRGITVLLATGRFYPAAAPYLEYWPGRPIWLAPCNGAQLFAPGQTEPITQRTLDLDLAVEVLSWIDRSRIMARVYFTTLALINRMTERTLAFMRRLGAQCRYEPALASAVTEPPTKIVLMGDPAEMPVVEAEVRSRWGGRLEVTSSEPDLVELTAPGATKGEVVRALAARLGIDRTQVAAIGNERNDLSMIHWAGLGAAVANAHEAVLAAVPRVVAHHDEDGVGQFIESFLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R97</accession>
    <name>Q67R97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH811</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH811"/>
    <dbReference type="EnsemblBacteria" id="BAD39796">
        <property type="protein sequence ID" value="BAD39796"/>
        <property type="gene ID" value="STH811"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH811"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_12_2_9"/>
    <dbReference type="OMA" id="QFVVICQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="7">
        <location>
            <begin position="62"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="6">
        <location>
            <begin position="283"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="458" mass="52836" checksum="4AD9B90D46407B62" modified="2004-10-11" version="1">MSNVFIMSKKTYRPYQPNQLLLLPPALQDWLPHDHFAYFLSDVVDSLDLSQIERTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMSYSRMVEEEQRLKAEIAELLQKAEQADQAEDHRFGPHRRGDELPEELRFRERRLAKIQEAKAALEAEARLRAGADEHEPPEEPGPKGPKGGRRRKRPKAEPAPTAQRNFTDPDSRIMKGSDKQFIQGYNAQAVVDADTQIVVAAAVTNQAADAPHLIPMMEQVETNVDEMPEEVSADAGYFSEQNVQWLLQRRINPYIPPEKQRHSERRRAKSPRGPIPKGATLRDRMRRKLQLKRHAERYRKREQSIEPVFGQIKEARGFRQFHLRGLEAVNGEWLLVCLCHNLLKLYAFSQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA5</accession>
    <name>Q67RA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative branched chain amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH803</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194936.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH803"/>
    <dbReference type="EnsemblBacteria" id="BAD39788">
        <property type="protein sequence ID" value="BAD39788"/>
        <property type="gene ID" value="STH803"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH803"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_7_0_9"/>
    <dbReference type="OMA" id="FVEMTII"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269332" evidence="4">
        <location>
            <begin position="28"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="5">
        <location>
            <begin position="65"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="428" mass="45950" checksum="3DA9FA288FC3539A" modified="2004-10-11" version="1">MRRWFTAILACALSLALIAGCSAPPEAESPGQTSGPSSSQPAGQTGGQSGETQPRREVQGVTDTTVKVGGFAAQSGPVAPIGISVRKGFEAYIKHVNDQGGVHGRKIEIVNIIDDQFDGSKALTAAKQLVESDKVFALAPILGTAGFMASYDYFVEKRIPVIYPMTGVEQTAHPPQENIFAVQPNNYDEAKILTQYLVDELGLKKIAVLWQNSDMGEQGLKGVKDYIGELGAELVYDAPHDAGSVDFTTPVLNLKQKGAEGVVIMTTLAETIGIVKEMHKQGVTAVPITSYVNADPVNVPALAGEAAIGLYAGGWVPVADPDDPNIQKFWEIYGRYNNGELPDAYGTAGFIAAELLVKGLEDAGRDLTYESFIQALENLNDWSGIMARNLTYGPGERAGVKAFYLNKVIEQDGNIVMVQVNQEPYRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC7</accession>
    <name>Q67RC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194914.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH781"/>
    <dbReference type="EnsemblBacteria" id="BAD39766">
        <property type="protein sequence ID" value="BAD39766"/>
        <property type="gene ID" value="STH781"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH781"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_9"/>
    <dbReference type="OMA" id="THANITF"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="292" mass="31959" checksum="2CCF5756EFD7A82E" modified="2004-10-11" version="1">MARFLVTGAAGFIGSHLVEALRAAGHDVVGVDRRPGADVVGDLLTLDLAPLLDGVEYVVHLAGQPGVRESWSQFPAYLAGNLQTTQRLLESLRDRPLKKFVLASTSSVYGEVPMPAREDGPAMPVSPYGLTKLAAEKLCDLYGRTAGIPWVALRYFTVYGPRQRPDMAFSRWFNAALDGEPIQIYGDGSQLRDFTYVADAVTATQRAALNPVVGVPINVGGGSAVTVREAIRLIAAITGRPIRIRQLPPAPGDMRETRADTERLWREVGFRPSTPLEEGLWQQYRWHLAQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE4</accession>
    <name>Q67RE4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH764</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the system for phosphate transport across the cytoplasmic membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH764"/>
    <dbReference type="EnsemblBacteria" id="BAD39749">
        <property type="protein sequence ID" value="BAD39749"/>
        <property type="gene ID" value="STH764"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH764"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_1_0_9"/>
    <dbReference type="OMA" id="GTKHGTR"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011862">
        <property type="entry name" value="Phos-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02136">
        <property type="entry name" value="ptsS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7 11" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PBP_domain" evidence="13">
        <location>
            <begin position="40"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD39749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="33949" checksum="3BAC24F4D2769A3B" modified="2004-10-11" version="1">MLTACGGRASSNPGSQPGAGNPPASHPGTNNPAPSSSGGSAGTELSGTVTLDGSSTVGPISIAVAEEFRKVHPRVDVPVGISGSSAGIGKFVKREVDIANASRPIKEKELEEARNNGLDPIELPVAYDGLSVVVNKENDFLTCITTEQLHKIWGPESTVTRWNEVDPSWPDAPIKLYGPGTASGTFEYFNEMVNGKGDASRSDFTASEDDNVLVQGVAGDKYAMGYFGYAYYAENKDTMKALAIDAGDGCVEPNDDTIAAGSYPFSRLIYIYPSRQALERPEVKAFVTFYMENAGELAAQVGYTPLPEEMYQDNLAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG7</accession>
    <name>Q67RG7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Copper-containing nitrite reductase</fullName>
            <ecNumber evidence="5">1.7.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH741</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Fe(III)-[cytochrome c] + H2O + nitric oxide = Fe(II)-[cytochrome c] + 2 H(+) + nitrite</text>
            <dbReference type="Rhea" id="RHEA:15233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(+)</name>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.1" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH741"/>
    <dbReference type="EnsemblBacteria" id="BAD39726">
        <property type="protein sequence ID" value="BAD39726"/>
        <property type="gene ID" value="STH741"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH741"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031740_1_0_9"/>
    <dbReference type="OMA" id="APCVGCK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001287">
        <property type="entry name" value="NO2-reductase_Cu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00695">
        <property type="entry name" value="CUNO2RDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02376">
        <property type="entry name" value="Cu_nitrite_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="84"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="355" mass="37802" checksum="EF746BA533948FC3" modified="2004-10-11" version="1">MALALGAAAWASHRTTAEELRDLAWRVENLTRTVKPATDFGSPREGAADIRRNPTDLPGPVARREPAHVKVELETVELDGVLADGTTYTYWTFNGTVPGPFIRVRAGDTVELTLKNAGNSANVHSIDLHAVTGPGGGAASTQVAPGESRTFTFKALNPGLYIYHCASPHIPTHIAQGMYGLILVEPENGLPPVDREFYVMQGEIYAAGRAKDGGHQPFAGDLLYREQPNYVVFNGAFQALTGDQAMTARVGERIRLFVGNAGPNPASSFHVIGEIFDVVYTEGGTTAAENIGVTLIPSGGAVIVEFTAEVPGRYQLVDHSISRAVDKGAVAYIDVVGDPNPEIYHDPAGHPMGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH4</accession>
    <name>Q67RH4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH734</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194867.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH734"/>
    <dbReference type="EnsemblBacteria" id="BAD39719">
        <property type="protein sequence ID" value="BAD39719"/>
        <property type="gene ID" value="STH734"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH734"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_2_9"/>
    <dbReference type="OMA" id="WLVINYW"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269339" evidence="1">
        <location>
            <begin position="36"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="40"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="177" mass="19810" checksum="EF9488D1C7A4C531" modified="2004-10-11" version="1">MTRYPRYKRVTSAITLLLLAAWLGYVLLWPNPNAAAGSGVPVGSVAPDFELKTVTGESYRLSDLRGKAVLLNFFATWCRYCEQEMPALEEAYRTYADQGFVVLAIDLDETDLAITAFRDEMGITFPILVDRGSNVARVYQIVPLPTTYFIDRSGVVRGKWTGPLTPEQLREALQLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK1</accession>
    <name>Q67RK1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched-chain amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH707"/>
    <dbReference type="EnsemblBacteria" id="BAD39692">
        <property type="protein sequence ID" value="BAD39692"/>
        <property type="gene ID" value="STH707"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH707"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_7_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="31"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="393" mass="41811" checksum="5B1947A2EE64D34B" modified="2004-10-11" version="1">MGALLCLALLAGCGGRGSGAGGSGGSAAQGPIKVGGIFDITGATGDVGKPHADGARAWFDYLNNELGGVNGRKVELVWGDYAYQVPKAVELYKKYVNEDKVVAILGWGTGDTEAMKELIAQDKIPYLSGSFAESLTDPSVTPYNFIVSSTYSQQARVLLDWIKEQKPDAKVGIIFHDSPFGQAPVADAKAYAQQIGLTWVGEAALPGAAADAVTQVLDLHNKGVEFIMIQNVVKGTAQVLKAVQQQGLRDKIQVVGMTYSVDETLLTAAGDAAEGFVGTPAFVFPYDEGPGIDEAVAYAEKIGLKREDLTQKWLQGWVFGRILTEAIRRAGEDVTGETLKAAFETFNELDLGGIGAPLTFTPTDHGGMDRARIYQVQNGRFVAITDFVEPKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK3</accession>
    <name>Q67RK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194838.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH705"/>
    <dbReference type="EnsemblBacteria" id="BAD39690">
        <property type="protein sequence ID" value="BAD39690"/>
        <property type="gene ID" value="STH705"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH705"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_1_9"/>
    <dbReference type="OMA" id="TTLYYDT"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="31184" checksum="49B78EE4FC27C0B6" modified="2004-10-11" version="1">MSSGLGFFLNLLVNGIVVGALYALVALGFVLIYKASGVINLAQGELVLLGAYIGLTFTTELNLPFALALLLTIAVSALVGVAIERAVLRPFIGEPPISIIMVTMGLSFLIKAVIHGIWGTDMRSFQGLFRSEPIVIGPVAISEVFLWGLVAALILLVLLNAFFKYSLLGIAMRATADDEQAALSMGISVKRVLAATWAVAAMVAAVGGIFLGNIAGVNPLLTSYGLKVLPVAILGGLDSIPGAILGGFLIGILEALTGGYLDQYLTGIKEVVPFVVMIVILLIRPYGLFGKEIIERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK7</accession>
    <name>Q67RK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sigma-54-dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH701</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH701"/>
    <dbReference type="EnsemblBacteria" id="BAD39686">
        <property type="protein sequence ID" value="BAD39686"/>
        <property type="gene ID" value="STH701"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH701"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_1_9"/>
    <dbReference type="OMA" id="TINCATI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030828">
        <property type="entry name" value="HTH_TypR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18024">
        <property type="entry name" value="HTH_50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="7">
        <location>
            <begin position="153"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="112"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="465" mass="51460" checksum="3452870844012999" modified="2004-10-11" version="1">MICGSAGGMCMAPLAETVVRALLDGLAEGVLAVAPDGRIRLVNGAMAQLLGTPRARLLDRRPADLEDGDKWVPPVLRRCLETGERQAMVLERAGRRLALTAVPLPEGWVLATARDITELDRLERQVERMTQERERYRHELAGLRAGQAGRAEVVGSSLAMQRVMDLIRRVAPVDSTILLLGESGVGKGVLAAEIHRLSPRRSGPFVRIDCAALPGSLLESELFDYSPGSFTGARREGKPGLIEQAHGGTLFIDEIGDVPLELQVKLLHVIQERRIIRLGAVTPTAVDIRIIAATHRDLEAMVAEGRFRADLYYRLNVVPVKIPPLRERPEDIPVLARTFLDRFCARYRVERTLTPEAVERLVAYSWPGNVRELENLVERLVVTLDEREIRPEHLPLPAPGGPPVQVREIVPLKEAVAALELELVGEAYRRFGSSVKVGRALGIHQTTAARKIREWKARQERTGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT7</accession>
    <name>Q67RT7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protease PrsW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH621</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protease PrsW family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194754.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH621"/>
    <dbReference type="EnsemblBacteria" id="BAD39606">
        <property type="protein sequence ID" value="BAD39606"/>
        <property type="gene ID" value="STH621"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH621"/>
    <dbReference type="eggNOG" id="COG2339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081250_0_0_9"/>
    <dbReference type="OrthoDB" id="1626415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023596">
        <property type="entry name" value="Peptidase_PrsW_arch/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026898">
        <property type="entry name" value="PrsW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36844">
        <property type="entry name" value="PTHR36844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13367">
        <property type="entry name" value="PrsW-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016933">
        <property type="entry name" value="PrsW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="29577" checksum="8A6BCA93895AFE3D" modified="2004-10-11" version="1">MLWYIRHLDKYEPEPWTHIAAAFVAGVLVTVPVYAVEVLLDQVVSFSGFMETVYVSFIVAAVTEETGKGIAAWAASWRRPEFNEVMDGIVYFGVTHMGFAVAENLVYVFLGGDIHTGILTALVRTTTAVPMHVIVGMIMGYHMGVVRFATTRREKVLHILEGWVLPVLLHGFYNLGSLNQDMALETLTDLIRYGFGTALLYAAVVALWMALLPRVRRAQQASPFRPIAAYPLVAAEMPCPGCGAVYPQGARYCPNCGVRLERGGAPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW2</accession>
    <name>Q67RW2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH596"/>
    <dbReference type="EnsemblBacteria" id="BAD39581">
        <property type="protein sequence ID" value="BAD39581"/>
        <property type="gene ID" value="STH596"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH596"/>
    <dbReference type="eggNOG" id="COG3250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2304621_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021243">
        <property type="entry name" value="DUF2804"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35868">
        <property type="entry name" value="PTHR35868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10974">
        <property type="entry name" value="DUF2804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="100" mass="11911" checksum="B9AAC8B434D6761B" modified="2004-10-11" version="1">MNENGFVVDGRVTKIHDDLNFEYDPADFMRPWRIRTRETDQVNLLFEPFFERVARTDALVVRSEVHQMIGRFSGWVTTEAGERIAIERMVGWAEEHRARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW3</accession>
    <name>Q67RW3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PrcB_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH595"/>
    <dbReference type="EnsemblBacteria" id="BAD39580">
        <property type="protein sequence ID" value="BAD39580"/>
        <property type="gene ID" value="STH595"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH595"/>
    <dbReference type="eggNOG" id="ENOG5033AUW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_918061_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025748">
        <property type="entry name" value="PrcB_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14343">
        <property type="entry name" value="PrcB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269647" description="PrcB_C domain-containing protein" evidence="1">
        <location>
            <begin position="49"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="PrcB_C" evidence="2">
        <location>
            <begin position="105"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="32108" checksum="2EE1F8E4879525DD" modified="2004-10-11" version="1">MARTRPLLICRPGLPAALPAALSKSRSRLLRTLFAALLALASACGSPAATGTFTTLARGTHSGITAQEAALITSLAEWEALWRRHASRFDPPPALPPVDFSRSSVVALFAGERPTGGYSLYITDVAPQGDSLRVTALELRPAPGRPVTQAVTQPYHVIAVPRVKKDTRLEVRWRVRTDSAGLPAGAVLLGPAHHPLDGDALTGLRRHPAAEPADHLMDLRAHDQRVGARHPLEEGLEEQVDLVGLPGADAPGAHEVRGVILEVQVVVDLRDPTVHHEAIFVHAGPVGPAAAQVEADRVAALHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ7</accession>
    <name>Q67RZ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoate--protein ligase</fullName>
            <ecNumber evidence="4">6.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH561</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-lipoate + ATP + L-lysyl-[lipoyl-carrier protein] = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194695.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH561"/>
    <dbReference type="EnsemblBacteria" id="BAD39546">
        <property type="protein sequence ID" value="BAD39546"/>
        <property type="gene ID" value="STH561"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH561"/>
    <dbReference type="eggNOG" id="COG0095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_0_2_9"/>
    <dbReference type="OMA" id="CYLIPYD"/>
    <dbReference type="OrthoDB" id="871298at2"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016979">
        <property type="term" value="F:lipoate-protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019491">
        <property type="entry name" value="Lipoate_protein_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="PTHR12561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10437">
        <property type="entry name" value="Lip_prot_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00545">
        <property type="entry name" value="lipoyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="8">
        <location>
            <begin position="26"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="327" mass="37137" checksum="D5A1CE693F7A2E2D" modified="2004-10-11" version="1">MIYLETGSTDPYFNLAFEEYVFEKLDPTKSYFILWQNENTVVVGRHQNTYEEINQRYVEEHGIRVVRRLSGGGAVYHDNGNLNYTFIVDKDAAPDFNFAVFTVPVIKTLERLGVKAEFTGRNDLTIDGRKFCGNAQYVRRGRIMHHGCIMLDSNLEVVVNALKVREAKFQSKGVKSVRSRVTTINAHAPRPIAMEEFKSLLKGYILEAEGLETMALTPEQLDEVRRLRDEKYATWEWNYGASPAYDVRLEERFDFGLVTVYLQADRGRIQAVKIYGDFFGSGELAELEAALVGLPLDGNLEAALEPLDVGRYIQGMTARDLARLLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S01</accession>
    <name>Q67S01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH557"/>
    <dbReference type="EnsemblBacteria" id="BAD39542">
        <property type="protein sequence ID" value="BAD39542"/>
        <property type="gene ID" value="STH557"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH557"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073294_1_0_9"/>
    <dbReference type="OMA" id="IDATVWN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041444">
        <property type="entry name" value="HTH_41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032791">
        <property type="entry name" value="YhfZ_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14502">
        <property type="entry name" value="HTH_41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14503">
        <property type="entry name" value="YhfZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_41" evidence="1">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="YhfZ_C" evidence="2">
        <location>
            <begin position="44"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="30579" checksum="4A889DE21978CBC0" modified="2004-10-11" version="1">MQDYARRCETGNGTVQNALRSLIEAGAVQVEARGHKGTFLVQADYRKLREIAGLASLLGLMPLPYTRRYEGLASGLYEAVRQSDLPFSIGYMRGARNRIAALHRGQADFAVLSRLSAELAVAEGGVVIVMGLGPQTFVSQHGILLARPEYTGITDGMRVGIDPQTLDQAWLTAAECRGRQVELVEIPYSHLLQRLREGQIDAAIWNLDELSSGTMEIYSRPLQSPEARRIAESSSEAVLVIDANRPDLERLLPEIIDPALVRRVQDEVLEGKRIPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA2</accession>
    <name>Q67SA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194590.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH456"/>
    <dbReference type="EnsemblBacteria" id="BAD39441">
        <property type="protein sequence ID" value="BAD39441"/>
        <property type="gene ID" value="STH456"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH456"/>
    <dbReference type="eggNOG" id="COG4392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157896_0_1_9"/>
    <dbReference type="OMA" id="SYIPICI"/>
    <dbReference type="OrthoDB" id="2078961at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="108" mass="11518" checksum="77DB60A6E63CB396" modified="2004-10-11" version="1">MTVRTEVLLLFLGMALVTYLPRMLPIVVLSRFRLPPLVLRWLEFIPAAVLSALLAQGLLLDGGQLSLPPGNPALLAALPAFAVAIWTRSLMGTVLAGIAAMALLRAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH4</accession>
    <name>Q67SH4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tungstate ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH384"/>
    <dbReference type="EnsemblBacteria" id="BAD39369">
        <property type="protein sequence ID" value="BAD39369"/>
        <property type="gene ID" value="STH384"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH384"/>
    <dbReference type="eggNOG" id="COG2998">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061511_0_0_9"/>
    <dbReference type="OMA" id="LADRGTW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="41"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="300" mass="32322" checksum="5CE731ED74954712" modified="2004-10-11" version="1">MVGCSSAGQQPSTEPQPTQQEQQAEQPSTQPETPAEPEKPATPENPELILATTTSTVDTGLLDVLVPMFEEKTGYVVKTVSVGTGQALKLGENGEADVLLVHAPESEKPLEEAGVVINRQLVMHNDFIIIGPPEDPAGVKGTTKAAEALKKIAEAGSLFISRGDESGTHKKELALWNEAGIDPRGNAWYQETGQGMGATINVANEKLGYTLTDRGTYLAQKKNIELEIVLEGDSPLLNIYHVMQVNPEKWPLVNAEGAKAFVEFMVDPEVQEIIRTYGVDKYGEPLFFPDAGKSVEELGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM1</accession>
    <name>Q67SM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194471.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH337"/>
    <dbReference type="EnsemblBacteria" id="BAD39322">
        <property type="protein sequence ID" value="BAD39322"/>
        <property type="gene ID" value="STH337"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH337"/>
    <dbReference type="eggNOG" id="COG3314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1000877_0_0_9"/>
    <dbReference type="OrthoDB" id="819609at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="278" mass="29843" checksum="F3974F9D5AD210B4" modified="2004-10-11" version="1">MAVGMFGMPALGHTMALAHYIAALLVGLTFRFYGIHEHERTAEPPREGNMLSRALAALIKARQEDGRPLGQMLGDAIKDSMRTMALICGYIMMFAVLARMIDVTGLFPYVSAPFRWLFAVLHIDPALVRAAVTGLLEIDLGTLAASRATDAPLAQQVAIAGAIIAWSGLSVHGQVASVLSGTDIRMGPYVAARLLHAVLAFIWTLVLLPMAGPLSLGAARVLPTLGGLPAALWQTPFWEHLMRGTRWSLVIPLCLALIGAGAALLTGGVRWVHFHTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN2</accession>
    <name>Q67SN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194460.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH326"/>
    <dbReference type="EnsemblBacteria" id="BAD39311">
        <property type="protein sequence ID" value="BAD39311"/>
        <property type="gene ID" value="STH326"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH326"/>
    <dbReference type="HOGENOM" id="CLU_908898_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="32597" checksum="F653BBE347C7FEA9" modified="2004-10-11" version="1">MPNPVREIMRIQDRRAALGSVPGQQQTISERSGWSSQVTGLGVDGHPLLGGAIQLVAGAGVTLTQDPVTGRIIISASGGGGGGVGYPRFDPDEPPAIPSQWDDEFNTSVLHQKWTPLAVHGQGGFQAGEVISMLSCYGGHNDVNPMALLQSAPSGPWVMTAKVINAQQYLNGGGGDGSACVGLAVAPAADQGDAMDTWVIGHYSNANTYIRSEIWAKPISWGGSKGMFTFSSPSAYLRIVWDGTRLSYWAAPDGLAWYQWVSPYAPNWTPGRIGITVRGAVGPSYVDWFRVTTSSSFTRTTRHSWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR8</accession>
    <name>Q67SR8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194425.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH290"/>
    <dbReference type="EnsemblBacteria" id="BAD39275">
        <property type="protein sequence ID" value="BAD39275"/>
        <property type="gene ID" value="STH290"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH290"/>
    <dbReference type="HOGENOM" id="CLU_2902612_0_0_9"/>
    <dbReference type="OrthoDB" id="1998386at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="62" mass="7046" checksum="819D406FE8454847" modified="2004-10-11" version="1">MYEYKVLMLSAAEQLEGALNRYAREGWRCRFQYVVEGFGGFTKLVVTLERKVGSVPDDPAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV4</accession>
    <name>Q67SV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194389.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH254"/>
    <dbReference type="EnsemblBacteria" id="BAD39239">
        <property type="protein sequence ID" value="BAD39239"/>
        <property type="gene ID" value="STH254"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH254"/>
    <dbReference type="eggNOG" id="COG2011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077375_0_1_9"/>
    <dbReference type="OMA" id="FGYQRFR"/>
    <dbReference type="OrthoDB" id="1464674at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="24386" checksum="1BE4F06E59F9582B" modified="2004-10-11" version="1">MAEPGTALKAAWAAFYADLGRMMLEATKETLYMVGVAGLFTVLLGLPLGVLLVITARDGLWEKPALNRILGVLVNIGRSTPFVILMVAVSPLTRAIVGKSIGTTAAIVPLVVAAVPFMGRVVEQALHEVGPGKIEAALAMGSSNWQVVTKVLLAEARPSLVRGTALMLVNLVGYSAMAGAIGGGGLGDVAVKWGYNRFQMDVMYVSLVILVIMVQVVQWIGDSLANRFSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ2</accession>
    <name>Q67SZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194351.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH216"/>
    <dbReference type="EnsemblBacteria" id="BAD39201">
        <property type="protein sequence ID" value="BAD39201"/>
        <property type="gene ID" value="STH216"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH216"/>
    <dbReference type="HOGENOM" id="CLU_181383_0_1_9"/>
    <dbReference type="OMA" id="FYFPITT"/>
    <dbReference type="OrthoDB" id="2032133at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021320">
        <property type="entry name" value="DUF2905"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36443">
        <property type="entry name" value="PTHR36443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11146">
        <property type="entry name" value="DUF2905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="7848" checksum="B93644F2402B5FDB" modified="2004-10-11" version="1">MGRTLMAIGALLLVVGAAIALFERFLPGIGRLPGDIIIRRGNFTFYFPIATSLLASIGLTLLFWLWQRLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T34</accession>
    <name>Q67T34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH174"/>
    <dbReference type="EnsemblBacteria" id="BAD39159">
        <property type="protein sequence ID" value="BAD39159"/>
        <property type="gene ID" value="STH174"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH174"/>
    <dbReference type="eggNOG" id="COG3877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132137_0_0_9"/>
    <dbReference type="OMA" id="YPTAKAR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018658">
        <property type="entry name" value="DUF2089"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09862">
        <property type="entry name" value="DUF2089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="133" mass="15005" checksum="2BC6D5C94E361D1A" modified="2004-10-11" version="1">MIGLRKWRMPSQCPACSGELTVTRLECSHCGTAVEGRFDASPFARLTPEQQEFVAIFLRARGNIKEVERELSISYPTVRARLDAVLEALGYRVDRAALEEETREKQARRKEVLDRLNRGEITAEEAVAALKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T73</accession>
    <name>Q67T73_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">proC</name>
        <name evidence="5" type="ordered locus">STH135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194270.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH135"/>
    <dbReference type="EnsemblBacteria" id="BAD39120">
        <property type="protein sequence ID" value="BAD39120"/>
        <property type="gene ID" value="STH135"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH135"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_2_1_9"/>
    <dbReference type="OMA" id="VSCGPAF"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="3">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="4">
        <location>
            <begin position="159"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="28453" checksum="05094E21734C2ADE" modified="2004-10-11" version="1">MAIGFIGMGNMGRLLVTALVRAGALQPDEVLIYSRSPEKRRRVTAALPGVQEAYGGGDLARRAGVVFLCVKPPETAGVLEEIGPYITADHLLVTINNTVTLAMLEARTPARAAKVIPSVVHAVRRGVSLLVFGGRCTHRDRALLMRLMGAVSRPFVVPEEAVRTASDLTACGPAFLSLVLHAMAEAARGKAPALSREAVMAMVRETARATCELMEQTGYDFADVAARVATPGGVAAAGVDALHPRLDGFWEAVLAATAAHEAAKRAQLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J44</accession>
    <name>Q67J44_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197436.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3325"/>
    <dbReference type="EnsemblBacteria" id="BAD42306">
        <property type="protein sequence ID" value="BAD42306"/>
        <property type="gene ID" value="STH3325"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3325"/>
    <dbReference type="eggNOG" id="ENOG50313RY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104063_1_0_9"/>
    <dbReference type="OrthoDB" id="1455540at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009665">
        <property type="entry name" value="DUF1256"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06866">
        <property type="entry name" value="DUF1256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02841">
        <property type="entry name" value="spore_YyaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="183" mass="18892" checksum="2453197B8945BAD6" modified="2004-10-11" version="1">MDDAGAPAVIAAAVERCAAGLIHRCDGLVALCIGTDRSIGDALGPLVGSRLAVALPEGVTVLGTLDKPVHAGNLTEALAWVARNFRRPLVLAVDACLGRAESVGFLAVGSGSLRPGAGVSKSLPPAGDVYLTGVVNVGGFMEYFVLQNTRLSLVMRMAQVAAAGLEQGLRALLMRHGPRRQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J92</accession>
    <name>Q67J92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3277</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3277"/>
    <dbReference type="EnsemblBacteria" id="BAD42258">
        <property type="protein sequence ID" value="BAD42258"/>
        <property type="gene ID" value="STH3277"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3277"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_6_9"/>
    <dbReference type="OMA" id="RVMHDLR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="22"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="342"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="376"/>
            <end position="383"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="589" mass="64855" checksum="34018E45387850D0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-11-22" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC7</accession>
    <name>SP5G_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative septation protein SpoVG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoVG</name>
        <name type="ordered locus">STH3241</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in septation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SpoVG family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD42223" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42223.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_070105471.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JC7"/>
    <dbReference type="SMR" id="Q67JC7"/>
    <dbReference type="STRING" id="292459.STH3241"/>
    <dbReference type="EnsemblBacteria" id="BAD42223">
        <property type="protein sequence ID" value="BAD42223"/>
        <property type="gene ID" value="STH3241"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3241"/>
    <dbReference type="eggNOG" id="COG2088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1495467_0_0_9"/>
    <dbReference type="OMA" id="CFAIRNI"/>
    <dbReference type="OrthoDB" id="1871825at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00819">
        <property type="entry name" value="SpoVG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007170">
        <property type="entry name" value="SpoVG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036751">
        <property type="entry name" value="SpoVG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38429">
        <property type="entry name" value="PTHR38429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04026">
        <property type="entry name" value="SpoVG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160537">
        <property type="entry name" value="SSF160537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000157215" description="Putative septation protein SpoVG">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="140" mass="14669" checksum="3D7FD46AF953971A" modified="2005-11-22" version="2">MKITNVRINQLVGEGKVKALVSVVFDGVLTVTDLKVVEGSKGLFVSMPSRKNKDGKFRDVVYPCTAEMRQAIQNAVLGAYDAMTRSGVAPQAGGLQGAEEPTAVEPAPQLQDESELPWEPGDDGEGARELAAVAGAVAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF1</accession>
    <name>Q67JF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3217</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197330.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3217"/>
    <dbReference type="EnsemblBacteria" id="BAD42199">
        <property type="protein sequence ID" value="BAD42199"/>
        <property type="gene ID" value="STH3217"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3217"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="RLHSGQM"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="348" mass="38478" checksum="69F72F8D7FCBFCED" modified="2004-10-11" version="1">MRSTLRLANLVKEFESQKRTVRAVNDVSLDFPAGAFVTLLGPSGCGKTTILRMIAGLETPTSGEIYLDDKPITHVPPNKRETAMVFQNYALFPHLTVFENVAYGLRLRKVPEAELKRAVHEKLEMMGLSNMADRQPNQLSGGQQQRVALARALVMSPKILLFDEPLSNLDAKLRIQVRQDIRELQRNLGVTTVYVTHDQEEAMAISDYVVIMNGGQVEQAGSPEEIYFQPRNSFVADFIGGVNLIPGRMTGPDEAELLGRKVQVRNPYGIAAGQEVKVAVRPEEVTLGDGPFTGTVRRAVFLGRVTEYVIQVGDQRLVAVMHGQHRRMPEGAETTFNFNAGAPLALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG1</accession>
    <name>Q67JG1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative iron hydrogenase small subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3207</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3207"/>
    <dbReference type="EnsemblBacteria" id="BAD42189">
        <property type="protein sequence ID" value="BAD42189"/>
        <property type="gene ID" value="STH3207"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3207"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_1_9"/>
    <dbReference type="OMA" id="HYAQEIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028431">
        <property type="entry name" value="NADP_DH_HndA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43342:SF2">
        <property type="entry name" value="PTHR43342:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="186" mass="19791" checksum="1F4531B814F12C6F" modified="2004-10-11" version="1">MRMTHACKGITPDDPRFAELDAFIQDGRSSADHLIAVLHKAQEIFGFLPEEVQRHVAAALHVPPSEVYGVVTFYNYFTLTPVGKYPINVCMGTACYVQGAGRLLSLFERELGIQPGQVTPDGLFSLEVCRCLGACGLAPVVTVAGEVHGKLTESDALKLVAELRQRAAAEQPQAGAAVAAVGGEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI8</accession>
    <name>Q67JI8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin converting factor-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3180"/>
    <dbReference type="EnsemblBacteria" id="BAD42162">
        <property type="protein sequence ID" value="BAD42162"/>
        <property type="gene ID" value="STH3180"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3180"/>
    <dbReference type="eggNOG" id="COG0314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069141_1_0_9"/>
    <dbReference type="OMA" id="SEWKGHQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="25319" checksum="8F11D5B837F6F73F" modified="2004-10-11" version="1">MKLFAAAADAVGRRTLEGDWAGRTTAQLLEILTQQYPNLARLAPVLSVAVNREYAPADRVLADGDEVALIPPVSGGADDPEPPLFAITTEPLSADEIAARVTNPHSGATLVFVGTVREWTRGRRTVYLEYEAYPEMAVAQMEQIGREIAERWPGARTAIVHRVGRLEIGEASVVIAVATPHRADAFEACRHAIERLKQIVPIWKKEVWEDGEAWVGSQIGPDWMHQPLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ3</accession>
    <name>Q67JQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197229.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3115"/>
    <dbReference type="EnsemblBacteria" id="BAD42097">
        <property type="protein sequence ID" value="BAD42097"/>
        <property type="gene ID" value="STH3115"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3115"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_0_1_9"/>
    <dbReference type="OMA" id="QVPPYEY"/>
    <dbReference type="OrthoDB" id="1422015at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="26454" checksum="A949E4A3903122F5" modified="2004-10-11" version="1">MSGQIEGRNPVLEALRSGRTINRILVAKGAREGSIREVIALARSQGVIVQEVARERLDAMARGRNHQGVIALAAAHRYYDLDEVLERAAASGEDGLLLVLDGIEDPQNLGSLLRTADAAGVHGVIIPERRAVGLTETVAKVSAGAVEYVPVARVTNIARTLEMLKERGYWVVGTHQEADELYYEARLTGPLAVVIGSEGRGMSRLVAETCDFTVRLPMRGHVTSLNAAVAGAILLYEVRRQRSAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR5</accession>
    <name>Q67JR5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADH dehydrogenase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH3103</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197218.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3103"/>
    <dbReference type="EnsemblBacteria" id="BAD42085">
        <property type="protein sequence ID" value="BAD42085"/>
        <property type="gene ID" value="STH3103"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3103"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_0_0_9"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="NADH_4Fe-4S" evidence="12">
        <location>
            <begin position="317"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD42085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="394" mass="41335" checksum="882F7A814F0A2E43" modified="2004-10-11" version="1">MTHLLIEPAQEGREDLAAYRARGGYAGLEAARTRGSGWVLEQVTRAGLRGRGGSGDGRPIGQKWQRVAASRVPERYVVANAAESQAVSRKDRYLLARFPHRVLEGLLIAAQALGAREAYLYVRGDSPEALDGARDAVAEAGAAGLLGGVSVTVQPSAPTAVSGEETAILDALEGLEGYPQPKPPRPEEIGLRGRPTLVQNAESLAAVAALFRLGVDRVRAVGTPTCPGTALFTVTGAVDRPGVYEVPHGTPLAQLLALAGAPPREEILAVLPGGLGSGPLRPDELDVPLTYEDLAALGSSLGNSAVVVFRRADATLPDLVRENVALLARGSCGQCRGCREGHEGLLRALEAGDATEARRRAEVLLYGRGNCAFPTGTARFALRALQEFPAFARP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT8</accession>
    <name>RS12_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpsL</name>
        <name type="ordered locus">STH3080</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197195.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JT8"/>
    <dbReference type="SMR" id="Q67JT8"/>
    <dbReference type="STRING" id="292459.STH3080"/>
    <dbReference type="EnsemblBacteria" id="BAD42062">
        <property type="protein sequence ID" value="BAD42062"/>
        <property type="gene ID" value="STH3080"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3080"/>
    <dbReference type="eggNOG" id="COG0048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_1_2_9"/>
    <dbReference type="OMA" id="SPALEKC"/>
    <dbReference type="OrthoDB" id="1707228at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000146333" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-methylthioaspartic acid" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="127" mass="13830" checksum="096CC001B6541AE1" modified="2004-10-11" version="1">MPTINQLVRQGRKKLTEKSASPALKGNPFKRGVCTVVKTTTPKKPNSALRKIARVRLSNNIEVTAYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGALDAAGVANRMQGRSKYGAKRPKAGKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV7</accession>
    <name>RS8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsH</name>
        <name type="ordered locus">STH3061</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197176.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV7"/>
    <dbReference type="SMR" id="Q67JV7"/>
    <dbReference type="STRING" id="292459.STH3061"/>
    <dbReference type="EnsemblBacteria" id="BAD42043">
        <property type="protein sequence ID" value="BAD42043"/>
        <property type="gene ID" value="STH3061"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3061"/>
    <dbReference type="eggNOG" id="COG0096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_0_2_9"/>
    <dbReference type="OMA" id="FDQGYIL"/>
    <dbReference type="OrthoDB" id="1692188at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000126504" description="30S ribosomal protein S8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <sequence length="132" mass="14835" checksum="060FD944A0DA11D4" modified="2004-10-11" version="1">MVVSDPIADLLTRIRNANLAYHDRVDVPGSKMKKAIVQIMKDEGFIRDFEWIDNGHQGVIRIYLKYGPNKTRVISGLRRISKPGLRVYAKRDQIPRVLGGLGIAILSTSKGVMTDKQARQQGLGGEVLCYIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY9</accession>
    <name>Q67JY9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DinB_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197143.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3028"/>
    <dbReference type="EnsemblBacteria" id="BAD42011">
        <property type="protein sequence ID" value="BAD42011"/>
        <property type="gene ID" value="STH3028"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3028"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1592188_0_0_9"/>
    <dbReference type="OrthoDB" id="2047691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="1">
        <location>
            <begin position="12"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="18058" checksum="2FF6353A12AF61BB" modified="2004-10-11" version="1">MLTSPADFARYFESVRGRTLAFIQAIPEDRMDFAPQPGKFTFGDLIRHIAATERMFVEGAIEGRWAYPGHGRELGSTKDEALGYLMGAHEEAMARLRAADPGVLQEKRTTPLGATVSAWRLLMMMTEHEIHHRSQIGQYLVALGLEPPQLFGLKLEDFLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K21</accession>
    <name>Q67K21_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagellar hook assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flgD</name>
        <name evidence="4" type="ordered locus">STH2996</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlgD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197111.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2996"/>
    <dbReference type="EnsemblBacteria" id="BAD41979">
        <property type="protein sequence ID" value="BAD41979"/>
        <property type="gene ID" value="STH2996"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2996"/>
    <dbReference type="eggNOG" id="COG1843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047535_1_0_9"/>
    <dbReference type="OMA" id="QMTENVR"/>
    <dbReference type="OrthoDB" id="709873at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005648">
        <property type="entry name" value="FlgD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03963">
        <property type="entry name" value="FlgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15380" checksum="41EC0DFB93529E77" modified="2004-10-11" version="1">MSVNGVQGVQPGTASGAGQTAESRRPTDQLGKMEFLQLLVTQLRYQDPLNPIDDREFMAQLAQFSALEQMTENVRWARLSYGLSLVGAAVKYWGTEGQPEIGIVYAVRIDSGEPLLNLGNREIRLEQVIEVAPPSFEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K29</accession>
    <name>Q67K29_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flagellar biosynthesis protein FlhF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Flagella-associated GTP-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">flhF</name>
        <name evidence="13" type="ordered locus">STH2986</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2986"/>
    <dbReference type="EnsemblBacteria" id="BAD41969">
        <property type="protein sequence ID" value="BAD41969"/>
        <property type="gene ID" value="STH2986"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2986"/>
    <dbReference type="eggNOG" id="COG1419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_11_4_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020006">
        <property type="entry name" value="FlhF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF3">
        <property type="entry name" value="PTHR43134:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03499">
        <property type="entry name" value="FlhF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="11">
        <location>
            <begin position="285"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54" evidence="12">
        <location>
            <begin position="286"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD41969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="478" mass="49468" checksum="8E8EE7EB3ED5C087" modified="2004-10-11" version="1">MKKYMAPTYAEALILAKNELGTDAVIVDSRKVRVGGIFGLFGRVMTELTVAADQRPQPYRGARPAARPRGATAPSGAPASAQPAPTVPQGISADAAPAVVHGGPAPAAAAVPQGVSAAAPVAPQGVSAAAVPQPVAGAAASASPASGPAPAPEPAAAPAAAVWPALSDAVPASPLSGIPLPGSPASAALAPGSADLAGLRREMADLRQAVTRLVEARPDPAALLELKGYARKVYERLLDAGVAEGLALAISRKVGTHATKGRALLRAELNQLMGPGAPIQAEAGRRRVVALIGPTGVGKTTTLAKLAAHFALERGLKVGLITADTFRIAAVDQLRTYADILAIPLYTVERPTDVHQALAHNADADLVLMDTGGRSHKDEERMAELQELLAEAQPDEVHLVVSLNTNPRDVRRMLERYLPLGVNRFIFTKADETSAPGLMLNIRAQWDLPISYVTDGQSVPDDLRPADSVDYSKLFMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K43</accession>
    <name>Q67K43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Flagellar motor protein MotB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">motB</name>
        <name evidence="8" type="ordered locus">STH2972</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MotB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197087.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2972"/>
    <dbReference type="EnsemblBacteria" id="BAD41955">
        <property type="protein sequence ID" value="BAD41955"/>
        <property type="gene ID" value="STH2972"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2972"/>
    <dbReference type="eggNOG" id="COG1360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_0_0_9"/>
    <dbReference type="OrthoDB" id="1619550at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025713">
        <property type="entry name" value="MotB_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13677">
        <property type="entry name" value="MotB_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="7">
        <location>
            <begin position="191"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="103"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="300" mass="31632" checksum="B6036103780717B6" modified="2004-10-11" version="1">MAAMNKKPSAAEEHGGGGHGSGTARWMVSYADFMTLMFIVFVVMFSFARVDTAKYQSLARSLGQALGTVGPDIAPLPTRGQEGTALPIIWPERPGSLPDVPDWPAHLIAPPEEEPTESAPVPPVEPPPAEAEPADEPTDVWTVLEPEPAPVPASPRVTAPVAEPDEMAEVAEVFRAMPGVKTGLLSVALEERGIVLSVAGSLLFEPGEYTIRPEAYPTLNEIADKLAGVELPIMVNGTADASHAAGSAEAFELAALRAGAVVRYFTETYGLPGGNFVYFGYGDGQSASDRVTIVVARKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K85</accession>
    <name>Q67K85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197046.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2930"/>
    <dbReference type="EnsemblBacteria" id="BAD41913">
        <property type="protein sequence ID" value="BAD41913"/>
        <property type="gene ID" value="STH2930"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2930"/>
    <dbReference type="eggNOG" id="COG4848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1408111_0_0_9"/>
    <dbReference type="OrthoDB" id="1012379at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010838">
        <property type="entry name" value="DUF1444"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07285">
        <property type="entry name" value="DUF1444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="21934" checksum="36D2432A73F57FD8" modified="2004-10-11" version="1">MKLMEQEFADIRGRLYPLMMDPVQIQAKGGGQMVNGQIAEGLYVIYGIEEPGDKIRYVTYQDLRDWGVSHTTVHITAVQNLKRLTEGKRVTKLDSAEGGRPMFIWALADGYDAARILLTEWLQDFAEAVPGRLVLGVPHRNWLVAVGDADEELLKVVARRVAHEHRHAGFPVSPYLYTFDGERIVRYVRRQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE5</accession>
    <name>Q67KE5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycine reductase proprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">grdE</name>
        <name evidence="4" type="ordered locus">STH2870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2870"/>
    <dbReference type="EnsemblBacteria" id="BAD41853">
        <property type="protein sequence ID" value="BAD41853"/>
        <property type="gene ID" value="STH2870"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2870"/>
    <dbReference type="eggNOG" id="ENOG502Z7RC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050376_0_0_9"/>
    <dbReference type="OMA" id="EYTPFSK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050485">
        <property type="term" value="F:oxidoreductase activity, acting on X-H and Y-H to form an X-Y bond, with a disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016585">
        <property type="entry name" value="Gly/sarc/bet_Rdtase_B_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015417">
        <property type="entry name" value="Gly_reductase_pB_sua/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09338">
        <property type="entry name" value="Gly_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011588">
        <property type="entry name" value="Gly_sarc_betain_red_a/b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="2">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Cys)" evidence="3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR011588-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR011588-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="434" mass="46920" checksum="E62C4750E911798F" modified="2004-10-11" version="1">MRRRRAMKLELGRIYIRDVRFGETTRVEGGVLTVNREELANLLLQDERLTAVELDIARPGESVRIIPVKDVIEPLVKVSGPGGVFPGFIGKVETVGEGRTHVLKGCAVVTTGRIVGFQEGLIDMSGPGAEYTPFSKLINVTVHCTPREGLTQHEHEAALRLAGLKAAAYLGEAGRHAEPDEVVTYEHLPLPEAIARYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKRILPTLISPTEVFDGAIVSGNCVSACDKNTTYHHQNSPVIEDLYARHGKDINFLGVVITNENVTLADKERSSSYAAKLVRMLGADGAIVTEEGFGNPDTDLMMNCRKLEEKGIKTVLITDEYAGRDGGSQSLADATPHADAVVTAGNANAVVVLPPMEKVIGYPEQVNVIAGGWEGSLRPDGSIEAEIQVITGATNELGFSRLSARTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH0</accession>
    <name>Q67KH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196962.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41828">
        <property type="protein sequence ID" value="BAD41828"/>
        <property type="gene ID" value="STH2843"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2843"/>
    <dbReference type="HOGENOM" id="CLU_1359817_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="20802" checksum="CD436E02E450FBCF" modified="2004-10-11" version="1">MQQPGMVTYSPQAPVQTQPQAALAAPVPFAAGPFGSAPPFAQTGLPVQGQQPNPAQVLTQQLVQTARSLEQLFPGYQALLSLLLEASSNAPSPALNEAVRSVEEGLYYHGAALGAIRRILCGEATPNVLMNLAAGFHGLMRTQPRVRAAAEQVLSLMPSARQSLTGTLVQSIGAADGVLAAAATAIQTLVGPQAWEAARTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L04</accession>
    <name>Q67L04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2657"/>
    <dbReference type="EnsemblBacteria" id="BAD41642">
        <property type="protein sequence ID" value="BAD41642"/>
        <property type="gene ID" value="STH2657"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2657"/>
    <dbReference type="HOGENOM" id="CLU_2036867_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="12814" checksum="0B5E1BD359C67A9D" modified="2004-10-11" version="1">MPPSTTSCWPVMNPDWSGSARKSAAAAMSSGRPTRPTGCWAWSSGRSSSPVTSIHPGRMAFTRTAGPSVMARAWVRARMPPLLAEYASVLGSDCSARVEAMLMIAAWPAARSSGAANRDMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L05</accession>
    <name>Q67L05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2656"/>
    <dbReference type="EnsemblBacteria" id="BAD41641">
        <property type="protein sequence ID" value="BAD41641"/>
        <property type="gene ID" value="STH2656"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2656"/>
    <dbReference type="HOGENOM" id="CLU_1844093_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="14552" checksum="8D17DA7C34D62330" modified="2004-10-11" version="1">MDSRSPNWEMPALFTSTCSAGPTCSRNRRTSSSWATSQRCISTPSTGSGSMSAPSTLAPRRANARAMARPMPRAAPVTATTLPANVFSIRLSSFRPEVRPEATPVIVCPTAGASRPARVLLSPSQTAPSTPSSIGMMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L63</accession>
    <name>Q67L63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196720.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2598"/>
    <dbReference type="EnsemblBacteria" id="BAD41583">
        <property type="protein sequence ID" value="BAD41583"/>
        <property type="gene ID" value="STH2598"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2598"/>
    <dbReference type="eggNOG" id="COG2414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_841798_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFOR_N" evidence="1">
        <location>
            <begin position="6"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="34622" checksum="359B740518704F9D" modified="2004-10-11" version="1">MTTWAYAEQILYVDLGRRTARVEPTPTALKRGYIGGVGFVASLLAEAPSARIVLATGPLSDAMAGRLAMGAVVDGRMVFSSMGGRMAAALKGAGYDAVVIDGRLEHPGVLVLEPEGVRILDGGHLWGLEVPDVARQLYLETGPYWATMVLGPAADHHVPFATMVHEGHFAGGSGVAAALGRTRIKAVMVRDTLLIPSRCTGCDLACPAKLSPNAGRAGDLGLDAPTAERLAALADALIQAGMLPEPIELLTEIAYRRGVGALFSDGEEAALRRLGPEAARFLPLLPPRKRRGGIGIADLLGTCQRVWRERPGEILRSALAATKGLLADAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE6</accession>
    <name>MENG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Demethylmenaquinone methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.163</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">menG</name>
        <name type="ordered locus">STH2515</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2-demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9539"/>
            <dbReference type="Rhea" id="RHEA-COMP:9563"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18151"/>
            <dbReference type="ChEBI" id="CHEBI:55437"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.163"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Quinol/quinone metabolism; menaquinone biosynthesis; menaquinol from 1,4-dihydroxy-2-naphthoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.163" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196638.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LE6"/>
    <dbReference type="SMR" id="Q67LE6"/>
    <dbReference type="STRING" id="292459.STH2515"/>
    <dbReference type="EnsemblBacteria" id="BAD41500">
        <property type="protein sequence ID" value="BAD41500"/>
        <property type="gene ID" value="STH2515"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2515"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_0_0_9"/>
    <dbReference type="OMA" id="VRNFENL"/>
    <dbReference type="OrthoDB" id="1431378at2"/>
    <dbReference type="UniPathway" id="UPA00079">
        <property type="reaction ID" value="UER00169"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043770">
        <property type="term" value="F:demethylmenaquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102094">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102955">
        <property type="term" value="F:S-adenosylmethionine:2-demethylmenaquinol-7 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102027">
        <property type="term" value="F:S-adenosylmethionine:2-demethylquinol-8 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000193339" description="Demethylmenaquinone methyltransferase">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01813"/>
        </source>
    </evidence>
    <sequence length="251" mass="28582" checksum="79E95F0D99ADA234" modified="2004-10-11" version="1">MAPGYQPPSPEEKEQYVRELFDKIAENYDAMNQVMSAGQWEKWHREFVAQTHFRPGDHILDVACGTGDLTLLDAAQVAPDGKVIGVDISEGMLEVGRRRVAASPYKDLITLQLGNAMDLPFPDNTFDGVTMGWAMRNVASIPRTLSEIYRVLKPGGRFICLEASKPFSRFIRFGFFVYWKTFLPLIDWFVVKAGRQAKVRPYTYLSRSLDNYPFPDQLEELFREAGFVETDYQLLMLGTVAIHVGTKRREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM0</accession>
    <name>Q67LM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196564.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2441"/>
    <dbReference type="EnsemblBacteria" id="BAD41426">
        <property type="protein sequence ID" value="BAD41426"/>
        <property type="gene ID" value="STH2441"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2441"/>
    <dbReference type="eggNOG" id="COG1239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_499587_0_0_9"/>
    <dbReference type="OMA" id="PAKFIMI"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="31"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="545" mass="57006" checksum="51A6FE708A4D4EA1" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ4</accession>
    <name>Q67LQ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196530.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2407"/>
    <dbReference type="EnsemblBacteria" id="BAD41392">
        <property type="protein sequence ID" value="BAD41392"/>
        <property type="gene ID" value="STH2407"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2407"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112782_0_0_9"/>
    <dbReference type="OMA" id="TRSAWIW"/>
    <dbReference type="OrthoDB" id="1515042at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="22097" checksum="26AD9F5ACBEAAAE8" modified="2004-10-11" version="1">MAARTIQDPPFARFLFDNTSSSWIWLLARLYLGWQWLQAGWHKVTDPAWMNGGTALQGFWARAVAVPDPPAKPAITYGWYREFLTFLLEGGHYTWFAKLIAVGEVLIGAALILGLLTGFAAFAGAFLNFNFMLAGTASTNPVLFTLAILLLMAWKVAGYWGVDRWLLPALGTPWDRRPAAPERFEPEPVSPADERPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW1</accession>
    <name>Q67LW1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2350"/>
    <dbReference type="EnsemblBacteria" id="BAD41335">
        <property type="protein sequence ID" value="BAD41335"/>
        <property type="gene ID" value="STH2350"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2350"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030024_3_3_9"/>
    <dbReference type="OMA" id="MSFQSHT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="57"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="25514" checksum="373B396F0A505B53" modified="2004-10-11" version="1">MLLYHHLEPGADGSNGAIISTEEFAAQMAWLKANGFTTITTAQLLAWLEDGEPLPDRPVMVTFDDGYRSNYLHAYPVLQQHGFSGVIFMVTGLAGQKVGRLEYLTWEDMHAMTASGVIEIQAHSHDGHRNIDGQPALVHWSEEEILADWQLLSEALAAAELPPATAYAYPFGAHDQECIDALRKAGVKLGFTVHHGHVTQDHDPLRLNRLVVYPGTDECAFARLVTGRAACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M48</accession>
    <name>Q67M48_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196386.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2262"/>
    <dbReference type="EnsemblBacteria" id="BAD41247">
        <property type="protein sequence ID" value="BAD41247"/>
        <property type="gene ID" value="STH2262"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2262"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="281"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="536" mass="60267" checksum="6E6155B61F91D299" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M58</accession>
    <name>Q67M58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2252"/>
    <dbReference type="EnsemblBacteria" id="BAD41237">
        <property type="protein sequence ID" value="BAD41237"/>
        <property type="gene ID" value="STH2252"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2252"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269532" evidence="2">
        <location>
            <begin position="25"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="840" mass="89945" checksum="1F5AA2A3E955C2DE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC2</accession>
    <name>Q67MC2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2186"/>
    <dbReference type="EnsemblBacteria" id="BAD41171">
        <property type="protein sequence ID" value="BAD41171"/>
        <property type="gene ID" value="STH2186"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2186"/>
    <dbReference type="HOGENOM" id="CLU_2195598_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="108" mass="11236" checksum="B383556516793CB2" modified="2004-10-11" version="1">MKQPVAFPGRKVFSSEVSLPPFAVPPSSTAHTRTVVEPLASCAGVKLRWPTSPSWVRVWGPASSRTVMSPPGVKSGGALTAALMVTSPYAIAGASLPQGPGPNRQTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME1</accession>
    <name>Q67ME1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196292.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2167"/>
    <dbReference type="EnsemblBacteria" id="BAD41152">
        <property type="protein sequence ID" value="BAD41152"/>
        <property type="gene ID" value="STH2167"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2167"/>
    <dbReference type="HOGENOM" id="CLU_2467875_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9331" checksum="03DB227D102FA1D8" modified="2004-10-11" version="1">MHGGVRGFLITLGIWVFLTLTHMTIEWIGGRSPLAMFAREPRARYIAITVAVVLALGTAIGVASDRVRRRAAQAAGAAGGKEESAWPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF3</accession>
    <name>Q67MF3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Uridine phosphorylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196280.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2155"/>
    <dbReference type="EnsemblBacteria" id="BAD41140">
        <property type="protein sequence ID" value="BAD41140"/>
        <property type="gene ID" value="STH2155"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2155"/>
    <dbReference type="eggNOG" id="COG2820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068457_0_1_9"/>
    <dbReference type="OMA" id="NLVEGTQ"/>
    <dbReference type="OrthoDB" id="1028277at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="5">
        <location>
            <begin position="18"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="26099" checksum="EEF303AAF31EF4F8" modified="2004-10-11" version="1">MVERQPHIQCRPGDVAPFVLLPGDPGRIHAIAAQLDRWEQIACNREYRTITGTFRGMPVSATSTGIGGPSTAIAVEELIRVGARVLIRVGSAGALQPGIRVGDLLVAVGAVREEGASAMYVPPAYPAVSDHAVLAALLKAAGDLGVTAHAGLVRSHDSFYTDREDELTRFWAGKGVLGSDMETATLLTLARLRGARAASVLNVVVPADGGLEEGINDLVAGKEAARQGETAEIRVALQALHALWTEESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH9</accession>
    <name>Q67MH9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41114">
        <property type="protein sequence ID" value="BAD41114"/>
        <property type="gene ID" value="STH2129"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2129"/>
    <dbReference type="HOGENOM" id="CLU_3066976_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="53" mass="6113" checksum="5B72E7C35C015769" modified="2004-10-11" version="1">MAHLDDPAADRPKGAAARSRTHASRMPSRPRRIVPPFPARCRRIWVFERYTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI0</accession>
    <name>Q67MI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41113">
        <property type="protein sequence ID" value="BAD41113"/>
        <property type="gene ID" value="STH2128"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2128"/>
    <dbReference type="HOGENOM" id="CLU_1659841_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="16037" checksum="2E921FDC50C8E801" modified="2004-10-11" version="1">MAAIAGVPRLPDGLSPPADRPAVDQAEADRVTLDVNELIARAAGEGPVHEAPAGGAGTVRVCIREEADGRRKAVVDLTEAPWVVPAVDPYALTELGLDSVVEFILWKAEGASGRLGRPAPPPRPGGVAGSAASRHPRGWAYPSAGGLPPSGSSGSPCAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM6</accession>
    <name>Q67MM6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Molybdopterin oxidoreductase membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NrfD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196207.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2082"/>
    <dbReference type="EnsemblBacteria" id="BAD41067">
        <property type="protein sequence ID" value="BAD41067"/>
        <property type="gene ID" value="STH2082"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2082"/>
    <dbReference type="eggNOG" id="COG5557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045348_3_2_9"/>
    <dbReference type="OMA" id="ILHGAEW"/>
    <dbReference type="OrthoDB" id="1903395at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005614">
        <property type="entry name" value="NrfD_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03916">
        <property type="entry name" value="NrfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="424" mass="46281" checksum="8B5DD3DD77959C8D" modified="2004-10-11" version="1">MSALTREEAALRLADQRPSPGPGRLLRAGWYLLLIALVAAGGVAIAHRTAHGLAATNLTSITPWGAWIAFYIYFVGLSAGAFLLSSLIYVFGMARFERLGRMALLTALVAMIVALTFVLLDLGRMERALSALVYFNWLSPLAWEMRFYVIYILLLAAELWFAMRADLVRRAERSRLARWLSFGSRSLDTGRDHRMLRILGTIGVPIAIFGVHGGTGTIFAIVKARGMWFSALTPIVFIVSALVSGTALLALLYILRQKALKRPVNAGMVADLGKLLGGFLLVDLGLLFYEFLVPLLSMAPHETSVVHLMMEGPYAWTFWILQLGIGMVAPAILLMTRLRESVPLTALACLMVVVGIVGVRFNIVVPPLIEPVLQGLPAGQYFPNAVEWWTSAGIIAMGLLLFSVAADLLPVDEEVHEIGGTAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ8</accession>
    <name>Q67MQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2050"/>
    <dbReference type="EnsemblBacteria" id="BAD41035">
        <property type="protein sequence ID" value="BAD41035"/>
        <property type="gene ID" value="STH2050"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2050"/>
    <dbReference type="eggNOG" id="ENOG50334G0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_826177_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025402">
        <property type="entry name" value="DUF4375"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14300">
        <property type="entry name" value="DUF4375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4375" evidence="2">
        <location>
            <begin position="196"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="36942" checksum="140E8DA218E751C2" modified="2004-10-11" version="1">MAPKWLRGRPALTVDLVEPLQEGPPGRIAGGGMGKRRALAGPVLPLPVFMPMYTSVPAKGSGRYTLQVYTKVTVSGPEPRKYRSTSRVTSALFKPIPTSRRPRSAPAAPGAVAREVPQVRRRIAIASLLAVALAAALFAGRSFAGLPGRPGVRWEARHIRIDEARLAAIDDVMDLIEPLWWTVNISSPEAYEASLAPFTRGQRLVYALQMYRLEVDNGGHGQFYGNSGGIVWRDALEGFELLGLRELADNLRASAALLGGDPPLDRGEREALLSELRPDFAEITEQFWKLEAQHDVDGAILAYARAHPEEFLFDGVVRVPTSDTVILRQFIIWFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR1</accession>
    <name>Q67MR1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome C peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2047</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="6">Binds 2 heme groups.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Binds 2 heme groups per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2047"/>
    <dbReference type="PeroxiBase" id="5021">
        <property type="entry name" value="SthDiHCcP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41032">
        <property type="protein sequence ID" value="BAD41032"/>
        <property type="gene ID" value="STH2047"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2047"/>
    <dbReference type="eggNOG" id="COG1858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034652_1_1_9"/>
    <dbReference type="OMA" id="YKFANVG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004852">
        <property type="entry name" value="Di-haem_cyt_c_peroxidsae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026259">
        <property type="entry name" value="MauG/Cytc_peroxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03150">
        <property type="entry name" value="CCP_MauG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000294">
        <property type="entry name" value="Cytochrome-c_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="9">
        <location>
            <begin position="41"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="9">
        <location>
            <begin position="195"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000294-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="34540" checksum="015A8280F0DBC255" modified="2004-10-11" version="1">MAVGCSSSSTKSTGQLTEVRQGSFATIVAAPENPDNPTTPEKVELGKLLFFDPRLSSSGVISCATCHNLGLAGTDRLPTSIGHGFQRTPRNAPTVLNAAFFQNQFWDGRAQGLEEQAKGPIQAEAEMNMPADMAVERIKSIKGYHEYFEKAFPGEEDPITFDNIVKAIAAFERTLITPNDALDRYLRGDKNALSPQAIRGMETFERIGCTSCHAGPALSSGAMMKFDWGSDPGKASWTNNPQDEKFFRVQTLRNVSLTGPYFHDGSVETLEEAVTIMARVQLGYELSDQERDDIVAFLRSLVGEMPQVEVPVLPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT2</accession>
    <name>Q67MT2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196152.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2026"/>
    <dbReference type="EnsemblBacteria" id="BAD41011">
        <property type="protein sequence ID" value="BAD41011"/>
        <property type="gene ID" value="STH2026"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2026"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_16_1_9"/>
    <dbReference type="OMA" id="WHNPVPC"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029401">
        <property type="entry name" value="Nudix_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14803">
        <property type="entry name" value="Nudix_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="38"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="170" mass="19073" checksum="BC28A6FEB79EEEEE" modified="2004-10-11" version="1">MEETLRFCAACGGRLELRFVAEEGKERLVCRACGRITYLDPKISACTVPVLDGRILLARRGIEPARGRWVFPGGYMERGETVPQAAERETFEEVGLRVRATRPVGIYSYPDSVVVVIVYHCEVLGGEPAPNSETLEVRLFSPDEIPWDELAFPSTRDALRDFLAQWGRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV2</accession>
    <name>Q67MV2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH2006</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196133.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2006"/>
    <dbReference type="EnsemblBacteria" id="BAD40991">
        <property type="protein sequence ID" value="BAD40991"/>
        <property type="gene ID" value="STH2006"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2006"/>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_9"/>
    <dbReference type="OMA" id="HECDRAG"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="13">
        <location>
            <begin position="192"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="252"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="391"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="506" mass="53339" checksum="5C6A96A0F7E300C7" modified="2004-10-11" version="1">MRRPGQWPLAVQLALAFALVTVLALGGSGALLLYQTTARLLEEREASVMAWGQAAAGLAAEYWADPQEGLTLAFYRFHQQSGMRPVAVDGQGVVVADITSETELLGQRLTHPEVRDALAGRATAGTRRLPGGEWVMYAGVPVVVGERLVGAVLVAADITSVREARDDLLRQLAWVAGAMALLAVGAGVMLARYLTRPLVRLREAVSCLARGRLETRVVPGGSSELLDLGRGFNRMAEELGRLDQQRRAFVADASHELRTPIAAIRALAEPLLSDRPVDPAIYKEHLQDIVHECDRAGRLVNALLELARLDMRAGARRAAGDPVELRRLVAEVVHALEPLAAERGVRLELQPGLPVTAPADPQLLEAVVGNLVENGIKYTPRGGSVQVAVGIGPAEPAPGPGGSEPDSGGWHGTVSGGDAGEATRGGAQPGQVAWVTVADSGIGIPPEHLPHIFDRFYRVDKARSRATGGAGLGLAIAAEAAARLGGRIHVASKVGEGSRFTLTLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY3</accession>
    <name>Q67MY3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter ATP-binding protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1975"/>
    <dbReference type="EnsemblBacteria" id="BAD40960">
        <property type="protein sequence ID" value="BAD40960"/>
        <property type="gene ID" value="STH1975"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1975"/>
    <dbReference type="eggNOG" id="COG2274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_61_3_9"/>
    <dbReference type="OMA" id="TEVELCP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13161" checksum="2C4A7056E50C8297" modified="2004-10-11" version="1">MAGCGVRRPGTLGGEWARVAIARGLVRRRGLVVLDEPTAAIDPVTEVELCPRMLEMGAGSTAIVVSHRLGVAPYSDRVLVPDQGRIVEDGSHDELLAQGGLYARLWNAQASWYTDRPVQAGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N64</accession>
    <name>Q67N64_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tatA</name>
        <name evidence="9" type="ordered locus">STH1894</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a complex with TatC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TatA/E family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1894"/>
    <dbReference type="EnsemblBacteria" id="BAD40879">
        <property type="protein sequence ID" value="BAD40879"/>
        <property type="gene ID" value="STH1894"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1894"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_6_0_9"/>
    <dbReference type="OMA" id="EFKSATH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018448">
        <property type="entry name" value="TatB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01410">
        <property type="entry name" value="tatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="7831" checksum="868F0124F1C40D87" modified="2004-10-11" version="1">MGNLGFTEILVIMIVALVVFGPNKLPELGRSMGQAIREFRKATQSITTEVTRVASDEVREKIDKAKSPADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N70</accession>
    <name>Q67N70_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196015.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1888"/>
    <dbReference type="EnsemblBacteria" id="BAD40873">
        <property type="protein sequence ID" value="BAD40873"/>
        <property type="gene ID" value="STH1888"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1888"/>
    <dbReference type="eggNOG" id="COG0389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505185_0_0_9"/>
    <dbReference type="OrthoDB" id="658705at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="539" mass="57978" checksum="7529CE28186542E4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND1</accession>
    <name>Q67ND1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1827</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195955.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1827"/>
    <dbReference type="EnsemblBacteria" id="BAD40812">
        <property type="protein sequence ID" value="BAD40812"/>
        <property type="gene ID" value="STH1827"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1827"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_0_0_9"/>
    <dbReference type="OMA" id="TYVADMD"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016211">
        <property type="entry name" value="Glu/Phe/Leu/Val_DH_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42722">
        <property type="entry name" value="PTHR42722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000188">
        <property type="entry name" value="Phe_leu_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="6">
        <location>
            <begin position="141"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="177"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="355" mass="39205" checksum="5F08357CFE30BEDF" modified="2004-10-11" version="1">MGIFEQMAQYGHEQLVFCYDKSTGLRAIIAIHDTTLGPALGGLRMWPYEREEDAITDVLRLARGMTYKNSAMGLNLGGGKAVLWGDPHTDKSEELFRAFGKFVETLGGRYITAEDVGTTVEDMNYVLMETDHACGRAEVSGDPSPVTAYGVFMGIKACVKQVFGTEELKGKKIAVQGLGKVGYHLCRYLHEAGAELIVTDIDPEAVERAKQEFGAKAVAPDEIYTAECDIFAPCALGAILNDDTIPGLKCRIVAGAANNQLKEPRHGNMLRERGILYAPDFVINGGGVINVAEEYHPQGYSRERALKRVETIYDKLLRVFQIAEQRNISTAEAADVMAEERMHRIHQLNRIYLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE7</accession>
    <name>Q67NE7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage V sporulation protein AE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoVAE</name>
        <name evidence="2" type="ordered locus">STH1811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195939.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1811"/>
    <dbReference type="EnsemblBacteria" id="BAD40796">
        <property type="protein sequence ID" value="BAD40796"/>
        <property type="gene ID" value="STH1811"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1811"/>
    <dbReference type="eggNOG" id="ENOG502ZRG2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1413368_0_0_9"/>
    <dbReference type="OMA" id="NGDTVYC"/>
    <dbReference type="OrthoDB" id="1784136at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025914">
        <property type="entry name" value="SpoVAE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14097">
        <property type="entry name" value="SpoVAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="222" mass="22965" checksum="82AE4A9925441E94" modified="2004-10-11" version="1">MLGVRGPSKAAASPTARLADPPPAGPPVHVSDPAPGGIGLKPPVRVILLTDGDSAARRVAENVAQRLGLRCISASAGNPTPLSGPELVALIKQAVHDPVLVMVDDKGDPGTGPGEEALAYICRHPDIRVLGAVAVASNTRARGVEVDVSIDREGRVRDGPVDKDGQPRRRGRLKGDTVDVLNRLSVPIIVGVGDPGKTGGEIDLEAGCEITARAIRQILERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE8</accession>
    <name>Q67NE8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">STH1810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195938.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1810"/>
    <dbReference type="EnsemblBacteria" id="BAD40795">
        <property type="protein sequence ID" value="BAD40795"/>
        <property type="gene ID" value="STH1810"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1810"/>
    <dbReference type="eggNOG" id="COG0019">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_1_9"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="OrthoDB" id="861683at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="37"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="47"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="290"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="443" mass="47584" checksum="92016011F6AB434C" modified="2004-10-11" version="1">MHFYGTASRNGQGHLTIGGVDVVDLAREFGTPLYVMDEELIRQNCRAYVAAFRDHYPDFDVAYASKAFLCTAMAALVAQEGLSLDVVSGGEIATALQAGFPMDRTYFHGNNKTAEEIRLALEAGVGRFVVDSLHELELLNRLAGERGTTARALLRVTPGVEAHTHEYISTGQLDSKFGIPIEGGQALDAARAALQADHVELLGFHCHIGSQIFDLTGYRVAADRMLAFAAAVREETGFTPSELSLGGGLGVRYVEGDRPLPPADLVACLAEAVRSGAAERALPLPKLIVEPGRSIVAEAGTTLYTVGTIKEIPGVRTYLSVDGGMGDNIRPALYHARYECAVANKLDSERTRIVTVVGRYCESGDVLLTDAAVAADVAPGDLLAVFATGAYNYSMASRYNRFPTPAVVFVRDGRADLVVRRETFADMAACDVLPPRFRPRAEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH9</accession>
    <name>RNY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease Y</fullName>
            <shortName evidence="1">RNase Y</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rny</name>
        <name type="ordered locus">STH1779</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endoribonuclease that initiates mRNA decay.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase Y family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NH9"/>
    <dbReference type="SMR" id="Q67NH9"/>
    <dbReference type="STRING" id="292459.STH1779"/>
    <dbReference type="EnsemblBacteria" id="BAD40764">
        <property type="protein sequence ID" value="BAD40764"/>
        <property type="gene ID" value="STH1779"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1779"/>
    <dbReference type="eggNOG" id="COG1418">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028328_1_0_9"/>
    <dbReference type="OMA" id="PHAILGM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00335">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017705">
        <property type="entry name" value="Ribonuclease_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022711">
        <property type="entry name" value="RNase_Y_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF15">
        <property type="entry name" value="PTHR12826:SF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12072">
        <property type="entry name" value="RNase_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03319">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000344957" description="Ribonuclease Y">
        <location>
            <begin position="1"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="207"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="333"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <sequence length="517" mass="58573" checksum="5D1FDFF6A144D53B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ3</accession>
    <name>Q67NQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1705"/>
    <dbReference type="EnsemblBacteria" id="BAD40690">
        <property type="protein sequence ID" value="BAD40690"/>
        <property type="gene ID" value="STH1705"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1705"/>
    <dbReference type="HOGENOM" id="CLU_668895_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="411" mass="42346" checksum="7402248B7B173B5F" modified="2004-10-11" version="1">MRALWTLFRQDLRSRWRLGHGHPRRRVRTALLLAAVALLHLDALTGQGPAPGEAGDLPGILGLAAAYAALEALALLSVALVGLVRGGRTAALAGYVALPLIGLPAAALGHALASLSLRVLGRGLTHLLVRALQLAALGAALAGLDLSGGFGWWAGPGHALARGAVAVLASTPLLVALAARLTAFRAHEGAGGRRSAEGIVLRPVGFSALLRKEMRLIVRDPRVWGRLLAAPVAAGAIVLSIFRSQPLLSDPAWTATALGVFCAGMVGMMTDVFTHGEADRVPLLRLSPTPLSRLIAAKTLPALPLVLLTAAAGGVFLAAEGIPGAPLLIAAGALAGTACAWFNVEYRFRMFTTRSTVRSYAAAFLMVLALFAASAAVLGHVSFGPVVGVTNLVLAGLLFAGAFMLAGRPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS2</accession>
    <name>Q67NS2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Aspartokinase</fullName>
            <ecNumber evidence="15">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">STH1686</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 15">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="15"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195814.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1686"/>
    <dbReference type="EnsemblBacteria" id="BAD40671">
        <property type="protein sequence ID" value="BAD40671"/>
        <property type="gene ID" value="STH1686"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1686"/>
    <dbReference type="eggNOG" id="COG0527">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_3_2_9"/>
    <dbReference type="OMA" id="IIQSQRC"/>
    <dbReference type="OrthoDB" id="1067792at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04261">
        <property type="entry name" value="AAK_AKii-LysC-BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041740">
        <property type="entry name" value="AKii-LysC-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10 15 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="17">
        <location>
            <begin position="271"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAD40671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="44864" checksum="88225A552BF0CCE1" modified="2004-10-11" version="1">MGLVVQKFGGSSVATAEKYRRVARRIAWKKRQGNDVVVVVSAPGDMTDDLIERARSITDRPSAREMDVLLATGEQISIALLTMALHELGVDAVSLTGPQAGFQTDDHHRAARIVNIDTARIRRELAAGRVVIVAGFQGMDDHGDITTLGRGGSDASAIALAAYLSADQCQIFTDVDGVYSADPRIVPTARRLDEISYDEVLELAAAGAQVMQLRSVELAKQYGVEFEVLSSLAPLPDEGGEERGTKVVAKLSPNNHRIVSGVAVDSKVAHITLLGLPDRPGVAYRAFKALGDARINLDMIVQSAGTGCGNGPTADISFTCARDDLETALEVCNRLLPEFPGARVVYDTDVAKVSIVGSGVASNYGVAARMFEALAEKDINIELITGSEIKISCLVRASRAMEAVRAVHDKFELGDVAPIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS5</accession>
    <name>Q67NS5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195811.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1683"/>
    <dbReference type="EnsemblBacteria" id="BAD40668">
        <property type="protein sequence ID" value="BAD40668"/>
        <property type="gene ID" value="STH1683"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1683"/>
    <dbReference type="HOGENOM" id="CLU_1146722_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269464" evidence="2">
        <location>
            <begin position="30"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="26051" checksum="729CF449B102EDDD" modified="2004-10-11" version="1">MKDRGRDRLRLALAAAAASAVLLTGPALAAGTAGSDPAKDAAERALDVYRGFVPWEDFGFASQEELRDAVVAEGVPVFRLDPSALDGRLHRLGDAIRDVRLVEFVIAGGDRAVTRLTLRRTEDGYERVGFGGDGEHLRRGLSLLPAPEGARLVLLGPAEFLYWSGEEAEYLVNINGFALAGLEPLRLYTPEEALPGLRQYARGLLGLPDEPGLPPAAAWTLAATGALFVGWVVHGFWQIARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY4</accession>
    <name>Q67NY4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195753.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1624"/>
    <dbReference type="EnsemblBacteria" id="BAD40609">
        <property type="protein sequence ID" value="BAD40609"/>
        <property type="gene ID" value="STH1624"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1624"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_9"/>
    <dbReference type="OMA" id="APVPWFP"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071793">
        <property type="term" value="P:bacillithiol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023881">
        <property type="entry name" value="Thiol_BshA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03999">
        <property type="entry name" value="thiol_BshA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="11"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="191"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="386" mass="42374" checksum="4F3B4E90CDDDFE88" modified="2004-10-11" version="1">MRIGIVCYPSVGGSGIVATELAAQLARRGHEVHLVSSAVPFRLNRFEERIRFHQVETPTYPLFQEPPYVLTLANKLVEVHRIAGLDVIHAHYAVPHATAAYLAREIIGAGGPRVVTTLHGTDITLMGADPSFTEIIAFSINRSDEVTAVSESLRTDTVTLLPVDRPVRVIPNFLDCARWRRQEAGELRRRLAPEGERLLMHMSNFRRVKRPWDVVEVFARVARRLPSRLILVGEGPELAPTLSLAARLGVRDRVLVLGNQEEVAPLLSAADLFLLPSEQESFGLAALEAMACGVPVVVSRTGGLPEVVAEGETGFLCRVGDVETMAERALQILEDGRLHARLSAAAVEWVRTHFCAERVVPQYEALYRTVTGQAAEDGDGEGRRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ5</accession>
    <name>Q67NZ5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="3">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Spermidine synthase</fullName>
            <shortName evidence="3">SPDS</shortName>
            <shortName evidence="3">SPDSY</shortName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">speE</name>
        <name evidence="8" type="ordered locus">STH1613</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195742.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1613"/>
    <dbReference type="EnsemblBacteria" id="BAD40598">
        <property type="protein sequence ID" value="BAD40598"/>
        <property type="gene ID" value="STH1613"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1613"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_0_9"/>
    <dbReference type="OMA" id="LWPGQSF"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="PTHR11558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="7">
        <location>
            <begin position="4"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-methyl-5'-thioadenosine binding" evidence="3">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Spermidine binding" evidence="3">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="31842" checksum="056D52668AD0BAEC" modified="2004-10-11" version="1">MNRGDWLIEEQTPSYQTRWRVREVLHEEQTPYQKLQVLELEDFGRALVLDDAVQTTVGDEFIYHEMIAHVPLFTHPHPERVLIIGGGDGGTAREVCRHESVQKVDMVEIDRAVIEACRKHLPEIACGMDHPKVNLVVGDGIAWVADHADEYDVIIVDSSDPVGPAKGLFNQAFYKNVFRALKADGLFVCQVLSPFFHQSLIRDVYGVVSAIFPITMPYLAVVPTYPSGLHCFMLGSKRHSPLREGVRTPAWPTRWYTPAVHRAAFQLPPVVAQLLEPSGSQAGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P83</accession>
    <name>TRUB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="1">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truB</name>
        <name type="ordered locus">STH1525</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195655.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P83"/>
    <dbReference type="SMR" id="Q67P83"/>
    <dbReference type="STRING" id="292459.STH1525"/>
    <dbReference type="EnsemblBacteria" id="BAD40510">
        <property type="protein sequence ID" value="BAD40510"/>
        <property type="gene ID" value="STH1525"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1525"/>
    <dbReference type="eggNOG" id="COG0130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_0_1_9"/>
    <dbReference type="OMA" id="ELQFIRW"/>
    <dbReference type="OrthoDB" id="1166299at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000121923" description="tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="241"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <sequence length="299" mass="31525" checksum="3E89DDC7DB3C4E6A" modified="2004-10-11" version="1">MARAQNEAASEVDGVLNLLKPPGMTSHDVVAFVRRALGVKKAGHTGTLDPGVAGVLPVCVGRATRLAEYIAGSDKAYRAEITFGVATDTQDGFGEVVAEADASHLTRGDVAYALTRFHGPIEQVPPMVSAVKVGGKRLYELARKGVEVEREPRRVFIHRLQLLDFRPGPRPVAYIDVVCSKGTYVRTLAHDLGRFLQVGAHLSYLVRTRSGPFVLAQAATLEELAAGKARLLPPAAALGDMPRVTVSGRAAARVLHGVAPAVRVEHPDGTTVAVVAANGALLALAEADGGGLRLRKVFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P85</accession>
    <name>RBFA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbfA</name>
        <name type="ordered locus">STH1523</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195653.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P85"/>
    <dbReference type="SMR" id="Q67P85"/>
    <dbReference type="STRING" id="292459.STH1523"/>
    <dbReference type="EnsemblBacteria" id="BAD40508">
        <property type="protein sequence ID" value="BAD40508"/>
        <property type="gene ID" value="STH1523"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1523"/>
    <dbReference type="eggNOG" id="COG0858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089475_6_3_9"/>
    <dbReference type="OMA" id="GDLQHCK"/>
    <dbReference type="OrthoDB" id="1971380at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000102753" description="Ribosome-binding factor A">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <sequence length="125" mass="14344" checksum="5DD113ECFA13AFEB" modified="2004-10-11" version="1">MSGNARAIRVGEQMREELAQLLRDEVKDPRIGFVSIVKVEVSRDIRHAKIYVSVLGNDQQKKETMAGLASATGFLRSELAKRLQLRYMPELHFALDESIEHGQRIAQLLVQVREEEKRSEREEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P99</accession>
    <name>Q67P99_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1509</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195639.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1509"/>
    <dbReference type="EnsemblBacteria" id="BAD40494">
        <property type="protein sequence ID" value="BAD40494"/>
        <property type="gene ID" value="STH1509"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1509"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_4_1_9"/>
    <dbReference type="OMA" id="CALLSCW"/>
    <dbReference type="OrthoDB" id="1421296at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="283" mass="29197" checksum="12EB5682630F2561" modified="2004-10-11" version="1">MIAALVIVAVGALVAGSAGLVGSFLVLRRMTMLGDAISHAVLPGIALGFLLTGSRAAPVMLLAAGLFGLLTAYLVQALQRAGVQEDAGMGVTFTAFFSLGVVLISAFAGRVHLDLDHVLYGEIAFAPWDLWVVGGRSLGPRALWTMGAVFLTDLAAVLLFFKELKICAFDPETASVQGVNVTAFHYLLMSLVSLTTVGAFDAVGAILAVAMLVLPGATAYLLTDRLETMLALSVGIGAVTSALGYLLARATDTPVAGAMAVAGGALFALAFGWSGRKRLVRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE7</accession>
    <name>Q67PE7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195591.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1461"/>
    <dbReference type="EnsemblBacteria" id="BAD40446">
        <property type="protein sequence ID" value="BAD40446"/>
        <property type="gene ID" value="STH1461"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1461"/>
    <dbReference type="HOGENOM" id="CLU_2276042_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="102" mass="11027" checksum="693E151A06982F3D" modified="2004-10-11" version="1">MARLDALFFQHIPPLRAAAVALPEGGVLFSDDGFADFLRTGRPTSDPAGPQVLSTGVAWEELRSAWSRVLPLEPFAHDGWLAHLQTYAGLDWSGLDALGLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH1</accession>
    <name>Q67PH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cell wall-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1437"/>
    <dbReference type="EnsemblBacteria" id="BAD40422">
        <property type="protein sequence ID" value="BAD40422"/>
        <property type="gene ID" value="STH1437"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1437"/>
    <dbReference type="eggNOG" id="COG3584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_862420_0_0_9"/>
    <dbReference type="OMA" id="WVSHREA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010611">
        <property type="entry name" value="3D_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06725">
        <property type="entry name" value="3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="49"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="122"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="286" mass="31170" checksum="C9414907F052EE75" modified="2004-10-11" version="1">MERLVAAGVIHGDPAGRFRPDAPISRAEFLKMLLTARRLDPAGKCAGLFADAQCWTWYAPYVELAYRLAIVEPKTDMLDDEPDYFDPEGAITRQEVVTALIRATGKRWTAQTMHWREASEILGRYADGADVTEPYRKPMALALTEGLVRGFGDGTLRPWHQVTRAEAAALVGRVLLDAPDLPTVSLDGHEVVYVDALDMRTTMYTTGEAGVGTRTATGVTVRPGAVAVDPAVIPLGTLLFVEGYGYAVAVDTGGAVKGNAIDLFDWVSHREALLFGIQTRRVWVLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ3</accession>
    <name>TRPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpA</name>
        <name type="ordered locus">STH1415</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195545.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PJ3"/>
    <dbReference type="SMR" id="Q67PJ3"/>
    <dbReference type="STRING" id="292459.STH1415"/>
    <dbReference type="EnsemblBacteria" id="BAD40400">
        <property type="protein sequence ID" value="BAD40400"/>
        <property type="gene ID" value="STH1415"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1415"/>
    <dbReference type="eggNOG" id="COG0159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_0_9"/>
    <dbReference type="OMA" id="LVMTYWN"/>
    <dbReference type="OrthoDB" id="912786at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000098858" description="Tryptophan synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <sequence length="279" mass="29484" checksum="19C83D7BCD7B6BA7" modified="2004-10-11" version="1">MSRIAERFALLRSRGEKGLIAYLAAGDPSLATTRRLVHALAEAGVDMVELGLPFSDPLADGPVIQAASQRALAAGANTDNVLELVAALRADGLQIPLLIMTYYNLVLRPGVENFCHRAAAAGVDGLILPDVPVEESDEIRAAAGAVGLDLIQFVAPTSPPERIRRAAELARGFIYAVSSTGVTGVRDRLPPQLTAMVEAVKAVTDTPVAVGFGISRPEQVRQVTAVADAAIVGSAFVRHCGEGLPEHELVERVRILAEELKAGTRPGTGWDSRFVKNEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ5</accession>
    <name>TRPF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="1">PRAI</shortName>
            <ecNumber evidence="1">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpF</name>
        <name type="ordered locus">STH1413</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195543.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PJ5"/>
    <dbReference type="SMR" id="Q67PJ5"/>
    <dbReference type="STRING" id="292459.STH1413"/>
    <dbReference type="EnsemblBacteria" id="BAD40398">
        <property type="protein sequence ID" value="BAD40398"/>
        <property type="gene ID" value="STH1413"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1413"/>
    <dbReference type="eggNOG" id="COG0135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_1_0_9"/>
    <dbReference type="OMA" id="FYAKSPR"/>
    <dbReference type="OrthoDB" id="1854712at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_1000197131" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <sequence length="222" mass="23155" checksum="EB3C66A20ECED55D" modified="2004-10-11" version="1">MWVKICGLQFMKDAVAAVEAGADALGFVFAPSRRQVTPERVEALISGLPPETVTVGVFVDAPMEEIRRAVTLSGLKAVQLHGSEPPEAIDQIGLPVIKAIRIRGPEDLARLPDYRNAAGLLLEPYVEGQAGGTGQTLDPTLVRWAAQTLERAGVPLAGPDEPLTPGRPKLILAGGLTPDNVADAIARAQPGGVDVSSGVENGGVKDINKIYAFVAAAKGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK5</accession>
    <name>Q67PK5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195533.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1403"/>
    <dbReference type="EnsemblBacteria" id="BAD40388">
        <property type="protein sequence ID" value="BAD40388"/>
        <property type="gene ID" value="STH1403"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1403"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044146_0_3_9"/>
    <dbReference type="OMA" id="YIYSSCK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="29644" checksum="386DBD897990EDD8" modified="2004-10-11" version="1">MQYDLVISDMDGTLLRDDKTISDRTKEAIRRFEAAGGRFSFATGRGVHASRRYFEDLGLRTPLVLLNGSLLYDPVADRELMVRPLDPAAIAAVWPRLEEAGLHILVHETRRAVVREITPTIDEHLRLDGITVDVQPDLSPATCGDIVKILSIGEPEQLDRAEAAILAANLPVKLVRSFRTYLEVLPPTGGKGTGLRALLAHLGIPRERCLAVGDYLNDLDLLAEAGLAVVVRNAHPGVRAVAQRETLSNEEDGVAAVLDALVEGREIGFPIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL4</accession>
    <name>Q67PL4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1394"/>
    <dbReference type="EnsemblBacteria" id="BAD40379">
        <property type="protein sequence ID" value="BAD40379"/>
        <property type="gene ID" value="STH1394"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1394"/>
    <dbReference type="HOGENOM" id="CLU_2467874_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019618">
        <property type="entry name" value="Spore_germination_GerPA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10676">
        <property type="entry name" value="gerPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9465" checksum="0EBD81C52C63CA72" modified="2004-10-11" version="1">MRPRHMVTERGVALTIEFHIHMIKVNGIDSSSAFTIGTNLLIGFGSQSKSVSGGNAVTGDYSSLPSLVNAIDDRDYIDTPAWQVAQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP4</accession>
    <name>Q67PP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195494.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1364"/>
    <dbReference type="EnsemblBacteria" id="BAD40349">
        <property type="protein sequence ID" value="BAD40349"/>
        <property type="gene ID" value="STH1364"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1364"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_447535_0_0_9"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="405"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="610" mass="69493" checksum="72116D0F01C4415A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ8</accession>
    <name>Q67PQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195480.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40335">
        <property type="protein sequence ID" value="BAD40335"/>
        <property type="gene ID" value="STH1350"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1350"/>
    <dbReference type="eggNOG" id="ENOG502ZQTE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1057392_0_0_9"/>
    <dbReference type="OrthoDB" id="1962970at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269305" evidence="2">
        <location>
            <begin position="26"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="26935" checksum="15A8CFFC15690179" modified="2004-10-11" version="1">MRNHTRMLPIAATLLLALLAAGCAASPGDLASFEDAGSREIAHTGGREPAGGTLVDGGGNAAAWVDPDALPASGGRLAQAVDPDAPVSSGQLMPAPDAPIPSRPVGSGNGGASGGGSSGMEPGVIYPTAPAEGAGSSGFAGEVGDLRLLYCGEDKYVHGRGRDLDARKCLWDAYLSNRAAAFRTVAYTIEGDPIAYDLDALGPDRIQVLVDSEDRFGATGEFLYTCTAMEFTDDLGFVLKGCAPNEPEGSRAFLSEEGELYVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q36</accession>
    <name>Q67Q36_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoIIIG</name>
        <name evidence="8" type="ordered locus">STH1222</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sigma-70 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1222"/>
    <dbReference type="EnsemblBacteria" id="BAD40207">
        <property type="protein sequence ID" value="BAD40207"/>
        <property type="gene ID" value="STH1222"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1222"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_5_9"/>
    <dbReference type="OMA" id="CGVNTAK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014322">
        <property type="entry name" value="RNA_pol_sigma-B/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014212">
        <property type="entry name" value="RNA_pol_sigma-G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02980">
        <property type="entry name" value="SigBFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02850">
        <property type="entry name" value="spore_sigG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="6">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="29283" checksum="91FD94876C9D6C44" modified="2004-10-11" version="1">MINKVEIPGVNTAKLPVLTNAQMRELFVRMRSGEKAARDELITGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKAIDNFDLGQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRDALVNRLSREPTVQEIADELGLPLEEIVYALDSIQEPVSLFEPLYNDGGDPIYVMDQVSDESQQDSQWLEGISIRQALHRLNERERLILTLRFFEGKTQMEVAEEIGISQAQVSRLEKAALSHMRKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q49</accession>
    <name>Q67Q49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stage V sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoVE</name>
        <name evidence="8" type="ordered locus">STH1209</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1209"/>
    <dbReference type="EnsemblBacteria" id="BAD40194">
        <property type="protein sequence ID" value="BAD40194"/>
        <property type="gene ID" value="STH1209"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1209"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_0_1_9"/>
    <dbReference type="OMA" id="HDYQKKR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013437">
        <property type="entry name" value="FtsW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02614">
        <property type="entry name" value="ftsW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="366" mass="39602" checksum="8B11C99071A9EBC1" modified="2004-10-11" version="1">MHAQRRSPDYTLMAVVALLLGIGIVMVYTSSTAIAEADFGNRYYFLVRQAIWVGIGLGAMAFFAGVNPWYWQKHSRTALLVAVVLLLLVLIPGIGISRLGARRWLGYGQLAFQPSEVAKFAYIMWLSTYLARYARDVTDFVRGLLPPVMVMGLLFGLIMLQPDLGTSLTLAGTGVLMLFAAGARLTHLAGLGVLGAAGVFVLARIDEERWSRITTFLNPWADPTDSGYQIIQALLAFGSGGLFGVGLGESRQKYFYLPERHTDMIYAVLGEELGFIGAALVLLLFFAFAWRGYRIAIQAPDRFSSLMAAGVTSLITLQAALNIAVVTASIPSTGIPLPFLSYGGTSLVITLSGVGILLGISRFCRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q60</accession>
    <name>Q67Q60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1198"/>
    <dbReference type="EnsemblBacteria" id="BAD40183">
        <property type="protein sequence ID" value="BAD40183"/>
        <property type="gene ID" value="STH1198"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1198"/>
    <dbReference type="eggNOG" id="COG4587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1146764_0_0_9"/>
    <dbReference type="OMA" id="VIEYNIL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="25950" checksum="7D088BC907CA0B52" modified="2004-10-11" version="1">MVVLYVFVQLWRTTFAAEGPVIDGYTLPEMIWYLVATEAIILSLPRVHAVLEQEVKDGDLALRLSRPYSYLGFHYASFLGEALVKLAVMLLVGGATAYLTVGPIPFRWGTAPLLLLLYLTTHALNFFYVATAGLLAFWAEDVLGIYLLLDRLKWILGGMLIPVGLYPEAVRRLVRWLPFRHMIGGPAELFVHFSWDGAAALLRQQALWLVVFGVICVGIYRLGVRRVDLNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q68</accession>
    <name>Q67Q68_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA carboxylase, biotin carboxyl carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1190"/>
    <dbReference type="EnsemblBacteria" id="BAD40175">
        <property type="protein sequence ID" value="BAD40175"/>
        <property type="gene ID" value="STH1190"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1190"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703343_0_0_9"/>
    <dbReference type="OMA" id="VTAVPHT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="3">
        <location>
            <begin position="75"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="14316" checksum="5C1188F1F3C81391" modified="2004-10-11" version="1">MDVEELTPAAGAGFSPAAPGPAGIAAAAGPSPAASAAAPAAPPVMAAPPAAVAPAPAAAPASAAASAPAPAAQPAASGGAAPAEGKAVCAPLPGMIVSVPVQVGQQVREGDVVAVLEAMKMENDITSPYAGTVKQVLVSKGTSVSMNDPLVVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ5</accession>
    <name>Q67QQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 3-hydroxyacyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1003"/>
    <dbReference type="EnsemblBacteria" id="BAD39988">
        <property type="protein sequence ID" value="BAD39988"/>
        <property type="gene ID" value="STH1003"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1003"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1427344_0_0_9"/>
    <dbReference type="OMA" id="EDVMPAK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="1">
        <location>
            <begin position="4"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="19759" checksum="470007F6E147F1E1" modified="2004-10-11" version="1">MERITVIGGTVAGVEIAALMARAGYATCLHEPDQAALAEAGRRLQDRLLGRQGEGGGAASVAQLAAVRVRLEAVPEVAVADADLVIEASSVDLPGKRELFARLDSFAPAHAILATCSPTISSAYLAAATSRPDRVVSLGFFSPPLAPPAVAVIQEPHLAPEVVAAVAEVVWRTGREPLLLRRGREPEGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT5</accession>
    <name>Q67QT5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195105.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH973"/>
    <dbReference type="EnsemblBacteria" id="BAD39958">
        <property type="protein sequence ID" value="BAD39958"/>
        <property type="gene ID" value="STH973"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH973"/>
    <dbReference type="HOGENOM" id="CLU_2002767_0_0_9"/>
    <dbReference type="OrthoDB" id="1927917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269941" evidence="2">
        <location>
            <begin position="29"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="124" mass="13429" checksum="AD206C281A785630" modified="2004-10-11" version="1">MYWLIMAHNVMRWVILVAAVATLAGALAAGKKAADGWAGRAAQAYTVALDVQVLIGLVIWLLRSGWNHDAFLAFIHPGTMILAMLVAHFGRTLQKRSVPVGGFVAFLVSLVLVIAAIPRWAWPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW1</accession>
    <name>Q67QW1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH947"/>
    <dbReference type="EnsemblBacteria" id="BAD39932">
        <property type="protein sequence ID" value="BAD39932"/>
        <property type="gene ID" value="STH947"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH947"/>
    <dbReference type="HOGENOM" id="CLU_1440386_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="188" mass="19976" checksum="680EECD09F0D2173" modified="2004-10-11" version="1">MQKAGTAYAAVPAFCLAAPALPSRYLSPMSSAVSDQPPGAELVLRHRRVGVGYGLWVPAAHRLPGRGSPFARAVGRSGRDGLRTGGFPVGRTAPIRRAHAQGLQGPGAFVRNRPAAGRPADSVPRSHHDHVAVGHHGLMCVRPFPGPRHARLTSWCSVCPSGSVPLPRARPAIRLPRGLTLTFFDLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX0</accession>
    <name>Q67QX0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxyisobutyrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH938</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH938"/>
    <dbReference type="EnsemblBacteria" id="BAD39923">
        <property type="protein sequence ID" value="BAD39923"/>
        <property type="gene ID" value="STH938"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH938"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_9"/>
    <dbReference type="OMA" id="AWVQSTT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="3">
        <location>
            <begin position="4"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="4">
        <location>
            <begin position="163"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="30863" checksum="D6AA34954D16074C" modified="2004-10-11" version="1">MMANLGFIGLGRMGRHMARNLIRAGHTVTLYNRSQAVVDELVAEGGRRAAGPAEVARDARVLFTCLTTPDVVESILRQALEGAQPGDIFVDHSTIGVRDAKRIAAMCAEKGVQFIDAPVSGGPWGAEAGTLTIMCGGDRAAFEAVLPYLQVEGKAIHHLGPVGAGSVAKLCNNLLVGIHTAAMAEAFVLGTKAGVDPKALYEIISNATGHSAQIARNIPQFVFPGKFDAAFSIDHLHKDVALAVELGKDENVRMVLGAVTQQLLAEARALGYGGSDQAALIRPLEELAGVKVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY0</accession>
    <name>Q67QY0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH928</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195060.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH928"/>
    <dbReference type="EnsemblBacteria" id="BAD39913">
        <property type="protein sequence ID" value="BAD39913"/>
        <property type="gene ID" value="STH928"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH928"/>
    <dbReference type="HOGENOM" id="CLU_1814842_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269325" evidence="2">
        <location>
            <begin position="25"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="14574" checksum="57BF98A44B4448B8" modified="2004-10-11" version="1">MSWKSVRDLLPLVVLHVAAAAVHAASAVGLSGVRPGAAFEVALAFVALVVPMAAVLALAAGMRRVGAALLVLSYLGVAGLTLVGHLGLNLLVNALDSAPSAFRTAFFASGLVMPVIQVCGVVEALRTWGLIAEPRRNRLSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ6</accession>
    <name>Q67QZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH912"/>
    <dbReference type="EnsemblBacteria" id="BAD39897">
        <property type="protein sequence ID" value="BAD39897"/>
        <property type="gene ID" value="STH912"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH912"/>
    <dbReference type="HOGENOM" id="CLU_2095634_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="12415" checksum="089545E2A8C39A86" modified="2004-10-11" version="1">MGACPSGICAGGAGVIPLLAARRFRSRVKGGGFGGGFREILGKEGREPVLKNVFRKLIGLLVVALFAVAVVQYFRQTSGNPYVWSALGSGLWILLPLAAVLSAVWWADRRMRARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R32</accession>
    <name>Q67R32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Death on curing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195008.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH876"/>
    <dbReference type="DNASU" id="2980986"/>
    <dbReference type="EnsemblBacteria" id="BAD39861">
        <property type="protein sequence ID" value="BAD39861"/>
        <property type="gene ID" value="STH876"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH876"/>
    <dbReference type="eggNOG" id="COG3654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115697_7_0_9"/>
    <dbReference type="OMA" id="AAYAFHI"/>
    <dbReference type="OrthoDB" id="1838027at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006440">
        <property type="entry name" value="Doc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39426">
        <property type="entry name" value="PTHR39426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018297">
        <property type="entry name" value="Doc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01550">
        <property type="entry name" value="DOC_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="1">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="14251" checksum="51677E03C23ADF3A" modified="2004-10-11" version="1">MLICLTADHLVGLHRRIIAETGGPHGVRDLGGVQSAAARPFRTYDGQALYPTLEEKAAALAEGISRNHPFVDGNKRVAIAAGCLFLMLNGREITASEQEMERTAWALAVREIDLQQLADWFRQHSREKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R63</accession>
    <name>Q67R63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH845</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194978.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH845"/>
    <dbReference type="EnsemblBacteria" id="BAD39830">
        <property type="protein sequence ID" value="BAD39830"/>
        <property type="gene ID" value="STH845"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH845"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="LVDMQGY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="130"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="24087" checksum="6C8315E5B7923193" modified="2004-10-11" version="1">MSKSLDELLVERAKRGDVEAFEQLISQHEKTVYNIAYRLTGNHEDASDLAQEAFIRAYSSLADFRGDSSFATWLYRIVNNVCLDELRRRKRQKVTYLDQAVEMEDGEVSRQIADTADGPEQALERVELQRLIQESILQLDEEYRVVLVMRELQGYSYNEIAEALDLNLGTVKSRLNRARSALKEKFMSLELLTPRVVYRARRGKAHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA6</accession>
    <name>Q67RA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH802</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194935.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH802"/>
    <dbReference type="EnsemblBacteria" id="BAD39787">
        <property type="protein sequence ID" value="BAD39787"/>
        <property type="gene ID" value="STH802"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH802"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_1_9"/>
    <dbReference type="OMA" id="QLAAYYM"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="289" mass="30513" checksum="C2CF632C3D42F494" modified="2004-10-11" version="1">MIFAQQIISGLATGSLYALAALGLVLIFKTTDVVNFAQGEMAMFGTFIMFTLLKTAGLPYWGAFVLALAFAFLMGAVIERVALRPLAQAPLISVLIATLGLMQIINGVAGWIWGYQARPFPTAVSGSPIRLGDLVITLPDLVNLAVSLVVMTGFFLLFKFTKLGIAMRAVAENRVAARLMGIPTDRILSLTWGLGGVLAATAGILIAPVTNLDINMMADMQIKAFTAAVLGGFTSLPGAVVGGLTLGVLENLVGQFVPQLKTPFAFGLIVLVLMLRPSGLMGTVQRKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA7</accession>
    <name>Q67RA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH801</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194934.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH801"/>
    <dbReference type="EnsemblBacteria" id="BAD39786">
        <property type="protein sequence ID" value="BAD39786"/>
        <property type="gene ID" value="STH801"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH801"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_2_1_9"/>
    <dbReference type="OMA" id="MRPCGDF"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="341" mass="36657" checksum="6ADCE82C06D9557E" modified="2004-10-11" version="1">MGKKLLFLLGALAVLALPYALPDEWSWLVLLTNRALILAIAAIGLNLLTGFAGQISIGHAAFMAIGAFASYGLTAKVGIPFWGALPLAGLISAAVGYALGFPALRLQGHYLAIATLGFGVAVPQILNLWKGLSGGWTGVKPAAPYLYGLYFKEDRPYYYLILIALALLTWVALNIARRRTGRAFIALRDSSVAAQAMGISLARYKTTAFALSAFYAGIAGSLWAHMMNYIAPHDFNLALSMELFTIIVLGGLASVPGSIVGSLFMSLMPKAVERISLALQRSGAFDHSPFLMNVFKNLNQVLTGVILVLVVLYLPLGLVDLWRRLQHWWAGRRHAAAGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC4</accession>
    <name>Q67RC4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39769">
        <property type="protein sequence ID" value="BAD39769"/>
        <property type="gene ID" value="STH784"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH784"/>
    <dbReference type="HOGENOM" id="CLU_1467500_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="17242" checksum="51BE5101EDC4179E" modified="2004-10-11" version="1">MDRQVAAGTVRLRWRDANVAQSGDLMARLLNLVQEEKRDMESLYAEEPVRAHLLQATGDDGSGDGSGSGDGSGGGSGDGSGSGDGSGSGDGSGADDADAAMTGGGSGGDEADADGSGADAGSGGEDGSGDEGVDATMTDDGSGADAGSGGEDGSGDEGVDATMTGGGSGGDADGADDDSFGSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL8</accession>
    <name>Q67RL8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH690</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194823.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH690"/>
    <dbReference type="EnsemblBacteria" id="BAD39675">
        <property type="protein sequence ID" value="BAD39675"/>
        <property type="gene ID" value="STH690"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH690"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="EHPRHVF"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="125"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="125"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="25347" checksum="C259C406EEFD84D8" modified="2004-10-11" version="1">MQPQHILVADDEPELIEILTDYLEAEGYAVTGVETGEEAVARVRDGAFDCVVLDVMLPGMSGFEACRAIRAFSDVPILFLSARGEDADKIRGLGLGGDDYVAKPFSPPEVVARVKALLRRYRPSAEQWRFGPLTIDPKGYRVWRDGREVAMTAKELELLIFLARHPGQVFSREQLYDRVWGDFGDPRTVTVHINRIREKIEADPSRPQFIQTVRGLGYRFTGVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN0</accession>
    <name>Q67RN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH678</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194811.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH678"/>
    <dbReference type="EnsemblBacteria" id="BAD39663">
        <property type="protein sequence ID" value="BAD39663"/>
        <property type="gene ID" value="STH678"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH678"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012341_2_0_9"/>
    <dbReference type="OMA" id="LIMWRLS"/>
    <dbReference type="OrthoDB" id="856767at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="727"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="760"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="817"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="20"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="281"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="852" mass="90906" checksum="A5C5AED00DAEABF8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT4</accession>
    <name>Q67RT4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH624"/>
    <dbReference type="EnsemblBacteria" id="BAD39609">
        <property type="protein sequence ID" value="BAD39609"/>
        <property type="gene ID" value="STH624"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH624"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_2_9"/>
    <dbReference type="OMA" id="QWEVINI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="17"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="35620" checksum="E2F62B7A3B7183F6" modified="2004-10-11" version="1">MLPIHQITLPLPGGPGAVHVYAVRSDPVTLIDTGVNTPDARAALVDGLKRLGLRVRDVRRVLLTHAHVDHIGLAGWVQEESGAEVHLHPEEAGKAEVAPWWQEARDRILAEAGVDPEGFALIERFWRLNRQLILPLQSWRQLADGQVFAFDGGRLEAVHLPGHALGHTGFLDREGRRLVGGDHLLDGITPNPILEPVPPGHPDAVPHAPNRALTLGQFLRSLDRAAGLDVDEVLPGHGPVITDHRAVGERYRAAHGRRLDSLLQRLQEGRSPWELTREVYPQVKGFDHFLALSEVLAHLDLLVVQGRALYTRKAGYGRYRAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT9</accession>
    <name>Q67RT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain fatty-acid-CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194752.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH619"/>
    <dbReference type="EnsemblBacteria" id="BAD39604">
        <property type="protein sequence ID" value="BAD39604"/>
        <property type="gene ID" value="STH619"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH619"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_9"/>
    <dbReference type="OMA" id="RVAAYKY"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="40"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="469"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="568" mass="63412" checksum="7463369C3FA1E4DE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV6</accession>
    <name>Q67RV6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glucose kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194735.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH602"/>
    <dbReference type="EnsemblBacteria" id="BAD39587">
        <property type="protein sequence ID" value="BAD39587"/>
        <property type="gene ID" value="STH602"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH602"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_0_2_9"/>
    <dbReference type="OMA" id="RRESQCR"/>
    <dbReference type="OrthoDB" id="1130699at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="32539" checksum="84F530C008259B3A" modified="2004-10-11" version="1">MVYLGIDIGGTGIKAAIVDGQGIILHRAEVPTGAAEGADAVLQRVRRLGLTLVERAGQPVEACGVGSPGRIDHLRGHVIFASGNLPGWTGTALGAELHQTFGVPVVVDNDVNAAAVGEAWIGAARGSSDFLMLTLGTGVGGALTVHGRLWRGARWGAGEVGHMALYPGGDPCPCGGRGCAERYVSSKALTRRANEGLVAGHPFRGVRDVIRTAEQGDGERQRAARLAVEQWTADLALFLMNLQMAFDPQMIVVGGGITRLGYWWDRLVQAAARECRERSMSIRIRRAKLGPMAGVVGAARLAMLRRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX8</accession>
    <name>Q67RX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative anthranilate synthase component I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194714.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH580"/>
    <dbReference type="EnsemblBacteria" id="BAD39565">
        <property type="protein sequence ID" value="BAD39565"/>
        <property type="gene ID" value="STH580"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH580"/>
    <dbReference type="eggNOG" id="COG0147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555392_0_0_9"/>
    <dbReference type="OMA" id="EWRECMA"/>
    <dbReference type="OrthoDB" id="1786019at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate_bind" evidence="2">
        <location>
            <begin position="215"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="491" mass="49953" checksum="D7AA9FBE459F6832" modified="2004-10-11" version="1">MATAESVRRLPLPPWEPADLAAALGSGPVALLDGITWEGRRVALVAWAPEARARTVAEAAAAADRTPLPPQAPPLLAAAVGRLDWDGTGRFWVPGSAALFDREAGELWVRGRLPAVPGRGCPPVRSGACAGEGGADASESSTNAGESGGTAGKSGGNTGENGANVRKSGADARKSGADEGEVGLAAGQGGSGRPGERASAHAPPHRAVPLWPYPAYAAAVREALTAMAEGRLTKVILSVPFAAPCPLPPAAIYRPLTAGSPPGLGFLLRVGAGGETLVGISPEPLVFLEAGRAHMHLLAGTRPAERASELAASPKDREEHAVAVEQARRDLEAVCLPGSVAVEAFMELERHPGLVHLASRLSGTLRPDLGPGDLVAACFPAGTVGGIPREAARALIDRLEPVPRGWYAGAVGAVLPGGGLQLWLTIRSLALRDGTVLVRAGAGLVPGSDPAAEWRECMAKAERALAAAGAALPPAEELMRGEDAVRGGDPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY8</accession>
    <name>Q67RY8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH570"/>
    <dbReference type="EnsemblBacteria" id="BAD39555">
        <property type="protein sequence ID" value="BAD39555"/>
        <property type="gene ID" value="STH570"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH570"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_5_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="41"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28863" checksum="6C9F5D481BFB16E6" modified="2004-10-11" version="1">MLLQMEGRRLVAPPYQPGQLGYVLGSDFLGRDYWSRLVYGARITVGLALMINLIRFALALPVGLQAGWRRGWLARAEQAAAAGFGSLPALVLVYIVLRGLWPFFLGGNAWMVAYVLVLGLAGAPRIAEQVRRRTEEIALMPHVEAAQALGARPARILRRHVLPIMRADLLVMAPSEMAWVLMIMAQLAVFGVHPGGAVQVETISGNIAVAEYLPEWGQMLGTNIPYLLSYPWLPFYPGLAIGLTAAGFHLLAEGLRERDLMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S64</accession>
    <name>Q67S64_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="4">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="4">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="4">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mobA</name>
        <name evidence="6" type="ordered locus">STH494</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MobA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH494"/>
    <dbReference type="EnsemblBacteria" id="BAD39479">
        <property type="protein sequence ID" value="BAD39479"/>
        <property type="gene ID" value="STH494"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH494"/>
    <dbReference type="eggNOG" id="COG0746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_2_1_9"/>
    <dbReference type="OMA" id="LFNCNTP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="5">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="21687" checksum="D9DCC8AE86E5D06B" modified="2004-10-11" version="1">MDACGVILAGGRSSRMGRNKALLALDGKPLIQRQVERLSAWFRQVVIVTNTPGEYAFLGVPMVSDRVPGLGPLAGVEAGLRASRFEHAFFCAVDMPFINEALVRFMVQAAPGYDIVVPAPGGEYEPMHAVYGRGCLPSIARNLEAGRLRLISIFPDVRVREITDAELLPFGEPERLFFNCNTPADLEEARRWLEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S88</accession>
    <name>Q67S88_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spermidine/putrescine ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH470"/>
    <dbReference type="EnsemblBacteria" id="BAD39455">
        <property type="protein sequence ID" value="BAD39455"/>
        <property type="gene ID" value="STH470"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH470"/>
    <dbReference type="eggNOG" id="COG1176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_3_9"/>
    <dbReference type="OMA" id="RIYAWMI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="51"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="29716" checksum="A4F83B6E984EC14C" modified="2004-10-11" version="1">MIWLVALVAAPMALMLVYSLAVQIAPDSPWPQGFTLAHYSRLLDPLYLRIFVRSVVQAALATLLSLLLGYPLAYYIARSPMRRRGRLLLLVVIPFWTNFLVRTYALMVLMRAQGVINSLLMAIGVIDDPLPLLYNEAAVQVGLVYTLLPLLVLPLYANLEKLDERLLAAAQDLGANPWTAFWSVTWPLSRPGVLVGGMMVFISAFGMYLVPDLMGGARSIMIGNLIQNQFLQARNWPFGAAVALALTAVVLVVALLVQRAGRIDGGEVKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S99</accession>
    <name>END4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable endonuclease 4</fullName>
            <ecNumber evidence="1">3.1.21.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endodeoxyribonuclease IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Endonuclease IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfo</name>
        <name type="ordered locus">STH459</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.21.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 3 Zn(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194593.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S99"/>
    <dbReference type="SMR" id="Q67S99"/>
    <dbReference type="STRING" id="292459.STH459"/>
    <dbReference type="EnsemblBacteria" id="BAD39444">
        <property type="protein sequence ID" value="BAD39444"/>
        <property type="gene ID" value="STH459"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH459"/>
    <dbReference type="eggNOG" id="COG0648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025885_0_1_9"/>
    <dbReference type="OMA" id="NPRGWAT"/>
    <dbReference type="OrthoDB" id="1088517at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008833">
        <property type="term" value="F:deoxyribonuclease IV (phage-T4-induced) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000190879" description="Probable endonuclease 4">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00152"/>
        </source>
    </evidence>
    <sequence length="276" mass="30064" checksum="E53141C1D8D773F4" modified="2004-10-11" version="1">MKLGCHISISKGFPQAVENAHRLGCEAFQFFTKNPRGFKGKSADPEAAARGRALMAEYGLVAVAHAPYITNLSTPDPELQAISIASLKQDLENAEAYGAIGCVCHMGKHVGEGEAYGRARMVETLNRLLEAYTGSCPLLLENTAGMGSELGTHLEELMEVRSRVEQPERIAFCFDTCHAFAAGIYRPEDWEDFVAHARAIGYWGLLRAVHLNDSKFDHGSRKDRHANLGKGFLGEAGIATLLRSGAFEGLPVVLETPVKDEAEYGPEIAYARSLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD4</accession>
    <name>Q67SD4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2344 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH424"/>
    <dbReference type="EnsemblBacteria" id="BAD39409">
        <property type="protein sequence ID" value="BAD39409"/>
        <property type="gene ID" value="STH424"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH424"/>
    <dbReference type="eggNOG" id="COG5011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083579_0_1_9"/>
    <dbReference type="OMA" id="QPCAILR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018768">
        <property type="entry name" value="DUF2344"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10105">
        <property type="entry name" value="DUF2344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03936">
        <property type="entry name" value="sam_1_link_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2344" evidence="1">
        <location>
            <begin position="8"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="25268" checksum="2DA9E68680339B5A" modified="2004-10-11" version="1">MWERTKLRLRIRLAKLGLARFLSHLDFQRVVERGLRRAGLPLAFTQGFNPHPRISYASALATGTSSEGEFIDVDLTEPVEPEAFVSRANAALPADVRLLEARPAPEGGESLMALVNAAEYRLTLQGADEEALRQAVARFLEAEQVVVTREGPAGSRSVDIRPHVYSLAVEGPGELRALVQTGSAGNVRPDEVVAGLIRCAPDLAGVELALAHRLMLYRRDPETGICAEPWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE6</accession>
    <name>Q67SE6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate dehydrogenase E1 beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194546.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH412"/>
    <dbReference type="EnsemblBacteria" id="BAD39397">
        <property type="protein sequence ID" value="BAD39397"/>
        <property type="gene ID" value="STH412"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH412"/>
    <dbReference type="eggNOG" id="COG0022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_9"/>
    <dbReference type="OMA" id="LPLDTCF"/>
    <dbReference type="OrthoDB" id="900125at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="326" mass="35555" checksum="EE0EA68DE7E2E6CB" modified="2004-10-11" version="1">MAKLTLIQAINDALRVAMRQDSTIVLLGEDIGINGGVFRATDGLIQEFGPERVIDCPLAESGYIGAAIGMAVNGLKPVAEVQFDGFLAPAHEQVANHLSRIRHRSRGRFTCPMVIRIPSWGGIKALEHHSESIENWYLNIPGLKMVAPSNPYDAKGLLLAAIADPDPVLYMEPKRLYRAFRAEVPEGYYTVPLGQAAVVREGTDMTVLTYGVHVHTALEAAEQAASQYGWQAEVIDLRSLNPLDLDTIIGSVKKTGRAVVVSEAPRTGGFHSELVALINDHALEYLEAPVARVTGFDVPMPYLLSEDLYIPDAGRVLEAMQAVRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI5</accession>
    <name>Q67SI5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Cell shape-determining protein MreC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Cell shape protein MreC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mreC</name>
        <name evidence="7" type="ordered locus">STH373</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in formation and maintenance of cell shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MreC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194507.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH373"/>
    <dbReference type="EnsemblBacteria" id="BAD39358">
        <property type="protein sequence ID" value="BAD39358"/>
        <property type="gene ID" value="STH373"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH373"/>
    <dbReference type="eggNOG" id="COG1792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042663_1_0_9"/>
    <dbReference type="OrthoDB" id="837894at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042177">
        <property type="entry name" value="Cell/Rod_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042175">
        <property type="entry name" value="Cell/Rod_MreC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007221">
        <property type="entry name" value="MreC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34138">
        <property type="entry name" value="PTHR34138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04085">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038471">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00219">
        <property type="entry name" value="mreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="62"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="31417" checksum="9023AB89E030DC5A" modified="2004-10-11" version="1">MRYNKHLRLLGVALGALVVIFITMALTARGRTAITPLEKGIATVLYPFQVATDWVRDRARGIVDSIRELTRLREENAALRQQVADMAQDRALIVQLQQENERLRKELGLKERTPYPMLTAEVISRSAESWYQTVIISRGSRDGVRQGMAVVNWQGLVGKIAYTTPYTSTVQLVSDVGFSEAGFTEAGFGAGAKLPNGEQGVLETVEGGYLRMRFWSTDPSVAVGQPVFTSGQGILPADLLVGWIEEVDTSSSFVKSALVRPAVDVHKLEVVQVVLTPSEDDRGSSAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI7</accession>
    <name>Y371_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>UPF0758 protein STH371</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH371</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0758 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SI7"/>
    <dbReference type="SMR" id="Q67SI7"/>
    <dbReference type="STRING" id="292459.STH371"/>
    <dbReference type="EnsemblBacteria" id="BAD39356">
        <property type="protein sequence ID" value="BAD39356"/>
        <property type="gene ID" value="STH371"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH371"/>
    <dbReference type="eggNOG" id="COG2003">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073529_0_2_9"/>
    <dbReference type="OMA" id="AMPDYEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08071">
        <property type="entry name" value="MPN_DUF2466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025657">
        <property type="entry name" value="RadC_JAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001405">
        <property type="entry name" value="UPF0758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020891">
        <property type="entry name" value="UPF0758_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30471">
        <property type="entry name" value="PTHR30471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04002">
        <property type="entry name" value="RadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00608">
        <property type="entry name" value="radc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01302">
        <property type="entry name" value="UPF0758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000070982" description="UPF0758 protein STH371">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="MPN" evidence="1">
        <location>
            <begin position="110"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="JAMM motif" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="234" mass="25615" checksum="DC734C688E5164E6" modified="2004-10-11" version="1">MTPSLKRLPEADRPRERLLRLGPEKLSDVELLAILLGTGSRGRSVIEVARDLLHHCEAKDPGGGLRALLHLRDAERSELVKGLGPAKVCTILAGLHLGLRASAAPLRRVDLCNPRAVFEFLAPRMAHLSIEQFHVILLNAKNQVIDVECVSEGTLTASLVHPREVFKTAIRRSAHAVILAHNHPSGDPTPSREDREITRRLVQAGRVIGIEVLDHLVVGQGGYTSFRERGLLTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL4</accession>
    <name>IXTPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">STH344</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194478.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SL4"/>
    <dbReference type="SMR" id="Q67SL4"/>
    <dbReference type="STRING" id="292459.STH344"/>
    <dbReference type="EnsemblBacteria" id="BAD39329">
        <property type="protein sequence ID" value="BAD39329"/>
        <property type="gene ID" value="STH344"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH344"/>
    <dbReference type="eggNOG" id="COG0127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_0_3_9"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000178248" description="dITP/XTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="151"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <sequence length="197" mass="21032" checksum="C47F230732797159" modified="2004-10-11" version="1">MSQKRLVLATKNQGKVREFRSLLAGAGFEIVGLDPDAPEVSETGDTFEENALIKARAASALTGLPALAEDSGIVVDALGGEPGVHSARWVPGSDEDRVRALLARMAEVPAERRTARYVSVIAVVLPSGREELFRGELEGRLAEAPRGTGGFGYDPIFVVADGRTVAEMALEEKNGISHRSRALARCLERLPALLEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP3</accession>
    <name>Q67SP3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH315</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH315"/>
    <dbReference type="EnsemblBacteria" id="BAD39300">
        <property type="protein sequence ID" value="BAD39300"/>
        <property type="gene ID" value="STH315"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH315"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="8">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="7">
        <location>
            <begin position="277"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="228"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="159"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="381" mass="43515" checksum="4133420DD7EBD576" modified="2004-10-11" version="1">MSKKTYRPYQPNQLLLLPPALQDWLPHDHFAYFLSDVVDSLDLSQIERTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMSYSRMVEEEQRLKAEIAELLQKAEEADRAEDDRFGPHRRGDELPEELRFRERRLAKIQEAKAALEAEARLRAGADEHEPPEEPGPKGPKGGRRRKRSKADPAPTAQRNFTDPDSRIMKGSDKQFIQGYNAQAVVDADTQIIVAAAVTNQAADAPHLIPMMEQVETNVDEMPEEVSADAGYFSEQNVQWLLQRRINPYMKPSTRNWSGTSWTRLTSRKTACASTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR6</accession>
    <name>Q67SR6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BcrAD_BadFG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH292"/>
    <dbReference type="EnsemblBacteria" id="BAD39277">
        <property type="protein sequence ID" value="BAD39277"/>
        <property type="gene ID" value="STH292"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH292"/>
    <dbReference type="eggNOG" id="COG2971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_1_9"/>
    <dbReference type="OMA" id="DTMGSMF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="1">
        <location>
            <begin position="8"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="30921" checksum="27B09D12D7290D03" modified="2004-10-11" version="1">METSRILVGIDGGGTRTRCLVATTDGRVIAEGGAGPANPLVVGLDRAVENIGQAVRQALEASGHTSAAVGAVCAGLAGAGQPETQARVAAALPGALSLSPGTPVQVVSDARVALAGALQGRPGAILIAGTGSIAYGLDDSGRLLRAGGWGWILGDEGSGFTIGRQALAAAFAAKDGTGPATRLGDRICAAWNLQDLTQAVPRIYADPSAARTAIAGLVPEVVQAAEEGDAVAAEILARAGGDLANLAAALLRRMQLREPLVATTGGVLEGVAAVRSELVRRLAELVPEARVVESAASPAAGAVLMARQMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY0</accession>
    <name>Q67SY0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative polyferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH228</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194363.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH228"/>
    <dbReference type="EnsemblBacteria" id="BAD39213">
        <property type="protein sequence ID" value="BAD39213"/>
        <property type="gene ID" value="STH228"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH228"/>
    <dbReference type="eggNOG" id="COG1148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048087_1_0_9"/>
    <dbReference type="OMA" id="CPDCRRY"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="271"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="348" mass="37105" checksum="597AA4E402551F56" modified="2004-10-11" version="1">MIDLERLLKLMDPDPIRVDAQACLAARHRHSSCRRCAEACPTSALRVEGGRVWADPAVCDRCGICAGVCPTAAVSVRGIDDEAVLSAAAVRCEKAAGDGLRLPCLGYLSVDHLLAMALRHGRVELAAGDCESCFRKVGGELAREAVALARAALAALGSDHAIRLTTEGGAEAATPSLTRRELLSLWRTESAQAARQLLPEPELNHARLPARLPRRRARWLRRLDPDATRAGEMPAGPWKARRATDACTGCGICAAFCPTGSLAAEGAEAGWTLTHQPAACVACGTCVDLCPARAMGEAPLAVEDLARGERRALIQLVECRCRHCRRTFRGRPGETQCPGCRNILGGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T03</accession>
    <name>Q67T03_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Superoxide dismutase</fullName>
            <ecNumber evidence="2">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH205"/>
    <dbReference type="EnsemblBacteria" id="BAD39190">
        <property type="protein sequence ID" value="BAD39190"/>
        <property type="gene ID" value="STH205"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH205"/>
    <dbReference type="eggNOG" id="COG0605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_891173_0_0_9"/>
    <dbReference type="OMA" id="TLDIWEH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="5">
        <location>
            <begin position="111"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="6">
        <location>
            <begin position="198"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="35484" checksum="01F27D0EBA50B259" modified="2004-10-11" version="1">MFGFDPYDSYALVCQHIADWARQARTAIACLKPEGGAADDLSGWQERFEALEREAQAALRADRSASGRPDAAALMRRAYEALTEFVRAAAGRGVPPAGELSPPRPVPPGGHRLPPLPYPYDALEPYIDAETMRLHHDRHHRSYVEGLNRAERALAEARAAGDWALVKHWERELAFNGAGHYLHTLFWESMAPGAGGEPGGEVLAQIRQDFGDFRRFREQFSKAAENVEGGGWAVWVWAPRANRTEILTAEKHQNLSQWDVVPLLPLDVWEHAYYLKYRNDRAAYIEQWWNVVNWPAVERRLREARRIAWEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T25</accession>
    <name>Q67T25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194318.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH183"/>
    <dbReference type="EnsemblBacteria" id="BAD39168">
        <property type="protein sequence ID" value="BAD39168"/>
        <property type="gene ID" value="STH183"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH183"/>
    <dbReference type="eggNOG" id="ENOG50331RK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1136633_0_0_9"/>
    <dbReference type="OrthoDB" id="1534253at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="23056" checksum="27D82A17AF61A131" modified="2004-10-11" version="1">MSCAYHPDREVRGICSHCGRPVCAECLVDLNGQPYCKRCLAARMQQPVREINGLVRFVLSIAPGVGHFYMGYFHRGTQLFLITLVGAAVLNLAFPGLLGLYIPAAIFFSIFDAREIHLRLSQGLEVEDRGFFDVQALPQRFGQRQAGIALIVVGALALWRVLTTDLLRWIFGANYFLVQRTLNGFTFGALALALGVWLLMRQPRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T69</accession>
    <name>Q67T69_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Cell division ATP-binding protein FtsE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">ftsE</name>
        <name evidence="8" type="ordered locus">STH139</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the ABC transporter FtsEX involved in cellular division.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Forms a membrane-associated complex with FtsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194274.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH139"/>
    <dbReference type="EnsemblBacteria" id="BAD39124">
        <property type="protein sequence ID" value="BAD39124"/>
        <property type="gene ID" value="STH139"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH139"/>
    <dbReference type="eggNOG" id="COG2884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="LPMYQRA"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005286">
        <property type="entry name" value="Cell_div_FtsE_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02673">
        <property type="entry name" value="FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25543" checksum="4D9246588A9A63BF" modified="2004-10-11" version="1">MIEFINVTKVYPHMKTPSLDGINLRVERGEFVFLVGPSGAGKSTLMKLIYREELPTTGQILVDGKDVARLPRRAVPYLRRSIGIIFQDFKLLPDRTVFENVAFALECTDVPRREVNRRVPAALELVGLRHKSRALAAELSGGEQQRVAVARAIVRNPVLVIADEPTGNLDPENTWAIMNLLNQINQMGATVVVATHAAEIVDTMKKRVIALERGRVVRDENRGTYGYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB3</accession>
    <name>Q67TB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycolate oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH95</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194230.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH95"/>
    <dbReference type="EnsemblBacteria" id="BAD39080">
        <property type="protein sequence ID" value="BAD39080"/>
        <property type="gene ID" value="STH95"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH95"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_1_0_9"/>
    <dbReference type="OMA" id="NSCLMHI"/>
    <dbReference type="OrthoDB" id="647535at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="133"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="27592" checksum="2C1B149D9C715B13" modified="2004-10-11" version="1">MRVSLFITCLADHFAPEVGEAVYRLLRRHGVEVDFPAGQTCCGQPSWNTGHLDDARDFARLYMKTFARSEYIVQPSGSCAGHVRYFYPEMFPDGSREQQEALAIGRRTYEFTEFMVKVLGVRDVGARFPGRAVFHNNCHMQRELRVQEEPVEMLRCVRDLELVEIPRPDLCCGFGGAFSVKEPEISTAMADDKLDAVLATGADLIVSSDLGCLMHQGGRIERRGLPIRTLHIAQLLWEGVQQNGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB7</accession>
    <name>ATPB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit beta</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">STH91</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TB7"/>
    <dbReference type="SMR" id="Q67TB7"/>
    <dbReference type="STRING" id="292459.STH91"/>
    <dbReference type="EnsemblBacteria" id="BAD39076">
        <property type="protein sequence ID" value="BAD39076"/>
        <property type="gene ID" value="STH91"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH91"/>
    <dbReference type="eggNOG" id="COG0055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_9"/>
    <dbReference type="OMA" id="GFNMIMD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000254403" description="ATP synthase subunit beta">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01347"/>
        </source>
    </evidence>
    <sequence length="472" mass="51358" checksum="B59B8C17DD50D769" modified="2004-10-11" version="1">MSEPTGKILAIMGAVVDVEFPDGHLPKLNHALVIRNGGERGDINLTLEVAQHLGNNQVRCIAMDSTDGLRRGETVYDTQAPITVPVGEPVLGRIFNVLGETIDGKGPVNSPVSLPIHREPPGVANVDPATEMLPTGIKVIDLLCPYARGGKIGLFGGAGVGKTVMIQELIHNIAYKFGGYSIFAGVGERTREGNDLYHEMIEAGVIDKVAMVFGQMNEPPGARMRVALTGLTMAEYFRDEQGLDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGYLQERITSTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAVFEKAIFPAVDPLDSTSRILDPNIVGREHYEVARGVQKVLQRYKELQDIIAILGMDELSDEDKLIVARARKIERFLSQPMFVAEKFTNLPGVFVDPKDTVKGFKEILEGKHDDLPEQAFYMVGTIEQAVEKGKKLLAEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI3</accession>
    <name>Q67TI3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH25</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH25"/>
    <dbReference type="EnsemblBacteria" id="BAD39010">
        <property type="protein sequence ID" value="BAD39010"/>
        <property type="gene ID" value="STH25"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH25"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15285" checksum="5E0F8DC91BD11A59" modified="2004-10-11" version="1">MAKIRVLVVDDHALVRTGLTKILSAQTDIEVIGEAESGNEARSLARSMPFDVMVLDISMPDLNGLQVAESLRREGCSGRIVFLSMYNKESFIFRALEAGALGYVSKSAPSDEVLRAVRWAHQGRIYLSPDSSTHIVAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI6</accession>
    <name>Q67TI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyanate hydrolase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH22</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH22"/>
    <dbReference type="EnsemblBacteria" id="BAD39007">
        <property type="protein sequence ID" value="BAD39007"/>
        <property type="gene ID" value="STH22"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH22"/>
    <dbReference type="eggNOG" id="COG1513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185569_0_0_9"/>
    <dbReference type="OMA" id="VYRFYEV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008824">
        <property type="term" value="F:cyanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009439">
        <property type="term" value="P:cyanate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00559">
        <property type="entry name" value="Cyanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008076">
        <property type="entry name" value="Cyanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003712">
        <property type="entry name" value="Cyanate_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036581">
        <property type="entry name" value="Cyanate_lyase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34186">
        <property type="entry name" value="PTHR34186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02560">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01693">
        <property type="entry name" value="CYANASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01116">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55234">
        <property type="entry name" value="SSF55234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00673">
        <property type="entry name" value="cynS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyanate_lyase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="73" mass="8538" checksum="81B8242F1752929B" modified="2004-10-11" version="1">MQPWPPTDPFVYRFYEVILLYGPVLKDVCHEIFGDGIMSAIDFSIDVKKVKGDEGEDRAQIILNGKWLRYKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J41</accession>
    <name>Q67J41_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197439.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3328"/>
    <dbReference type="EnsemblBacteria" id="BAD42309">
        <property type="protein sequence ID" value="BAD42309"/>
        <property type="gene ID" value="STH3328"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3328"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101313_1_0_9"/>
    <dbReference type="OMA" id="GCSVYAK"/>
    <dbReference type="OrthoDB" id="1893112at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027981">
        <property type="entry name" value="DUF4446"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14584">
        <property type="entry name" value="DUF4446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="18450" checksum="4D3C757D3729F345" modified="2004-10-11" version="1">MAVLLQQDPLLVAFTALGFCIVAIVIMLIVLVRQSILLRRYRSLLRGNTNASLEDLLIQQQQATADLRAAQESIRRRLSDLESASQKYLQRIGIVRYNAFPDVGADLSFSCALLDGEDNGVVVTSLYGRSECRTYAKPIRGGSSSYALTDEEKQALRLARGVDNEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J42</accession>
    <name>Q67J42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3327</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3327"/>
    <dbReference type="EnsemblBacteria" id="BAD42308">
        <property type="protein sequence ID" value="BAD42308"/>
        <property type="gene ID" value="STH3327"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3327"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_2_0_9"/>
    <dbReference type="OMA" id="HIISQME"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="3">
        <location>
            <begin position="243"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="38517" checksum="BC8B9BD20766F75F" modified="2004-10-11" version="1">MQLIVVPSTNRRSYSFFVYPALLAAGLGLVVLVVAALALGLRFFQTEAARLRAELAEMEALKQQARLQQEELERMRATAEQVEQELQEIRRLEKDLEHMTEGEDTLPSRSALTRRTPPGEGARGGPETSHSQQVPGLTLASILPDDVSPFVLGKRNTLAMDLKLGRLPSSMDSSLQSSRAVRAQLARQLAQLRDSVRALADGRDQLAAKLDYLAHKPAGWPVYGAEITDPFGPRWSPFGYGWQFHDGIDLGQDYGTPVYATGAGTVVYADWLEGGYGRCVIIDHGYGYRTLYAHLQDWNVFEGQEVERGDLIGWVGSSGLSTGPHLHYEVLVNGVAVDPEPYLESRPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J70</accession>
    <name>Q67J70_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3299</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
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        <property type="protein sequence ID" value="BAD42280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="BAD42280">
        <property type="protein sequence ID" value="BAD42280"/>
        <property type="gene ID" value="STH3299"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OMA" id="MEWVSFM"/>
    <dbReference type="Proteomes" id="UP000000417">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="9">
        <location>
            <begin position="172"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="511" mass="56683" checksum="44F8A430FFB7BCF3" modified="2004-10-11" version="1">MRKEARTLRILLVDDDREGRSYLARFLTLDGHAVTECGSAEEALEVYRPGAFEMVLSDIRMGRMSGINLVSAIRSQGGAPQADVVLYTGHLDISLAIAALRAGAFDYLTKPINMAELQAVLQRVEEHQRSLEEQRTPPPGYQGTGDDVPADVRLQMAYLKDLGAERAGQKKFCARSPAMRQVVEQARRYHTDRSLPVLIQGETGVGKEVIAHLIHYGDLEEAGPFVAINCSAITPTLFESELFGYEPGAFTGGSARGQKGKLDAAQGGTLFLDELAEIPVELQAKLLRVLEEKSFYRVGGLKKIDADVRIIAATNLDIEERIRQGLFRVDLYYRLNVCRIVIPPLRERKEDILPMALMYLIDFSRSRGRRFTGISDEAAEVLLSYHWPGNCRELRNLMEWVSFMYDDEVLRPEHLRRITDAAARAQVAAGTEPFGGGAPHGAAAPDSGDLPAATVVPFPRGSRRDYNLEHHTEALIQAALQEYGGNKTLAARALGISVRTLYNRLERMGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA9</accession>
    <name>Q67JA9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arginine/lysine/ornithine decarboxylases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3259</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197372.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3259"/>
    <dbReference type="EnsemblBacteria" id="BAD42241">
        <property type="protein sequence ID" value="BAD42241"/>
        <property type="gene ID" value="STH3259"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3259"/>
    <dbReference type="eggNOG" id="COG1982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025925_2_0_9"/>
    <dbReference type="OMA" id="YGADRTF"/>
    <dbReference type="OrthoDB" id="485164at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00615">
        <property type="entry name" value="Orn_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000310">
        <property type="entry name" value="Orn/Lys/Arg_deCO2ase_major_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008286">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036633">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01276">
        <property type="entry name" value="OKR_DC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03711">
        <property type="entry name" value="OKR_DC_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55904">
        <property type="entry name" value="SSF55904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00703">
        <property type="entry name" value="OKR_DC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OKR_DC_1" evidence="3">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="469" mass="49804" checksum="9E5F60F26096B201" modified="2004-10-11" version="1">MAEEQARTPLFSALREYVARVRAPLHVPGHKMGRAAPSAWREFLGPNALAIDLTEAPGLDDLHAPEGVIAEAQELAARAFGAWRSYFLVGGTTAGLHALILAACHPGEAIAVPRNAHRSVLGALILAGVRPHWVRVAFDPDLGIATGPVLTSLEEALSGAAAAVLVHPTYYGIAGDTAAAIDLIHRAAVPALVDEAHGAHFPFHPALPPSALELGADGVVQSLHKTGGSLTQSSLCHLGHGSRIAPERLQEMLRLVQSTSPSYLLMASLDLARRELALGGREAWGRALELAHEAKRRIDALPGLRVRPTDDPTKLLIDVRGRSISGFRAAERLWEEAGVAVEASGLTYVLAVLSPGDSREQIDALVAGLERLSSEGDVPALPPEPPWPEVVLPPREAYLARKKAVPLRQARGRIAAELVAPYPPGIPVVAPGERLTADVLDYLRRAADAGWHLQGPADPALRSIQVVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF5</accession>
    <name>Q67JF5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3213</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197326.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3213"/>
    <dbReference type="EnsemblBacteria" id="BAD42195">
        <property type="protein sequence ID" value="BAD42195"/>
        <property type="gene ID" value="STH3213"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3213"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_13_1_9"/>
    <dbReference type="OMA" id="CECKEVF"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="130"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="371" mass="41768" checksum="F5F6936F1A371F5F" modified="2004-10-11" version="1">MRFWQFLWDRINYIAVYVAFAALSLVVVHLDLMLTGASLQVVNLLYIFLLGLVGLVVYLWWEYQRIVPFYRHLAGLTGREPIAQLALLPVPQTAEGELLAEAWSRMYGRLSGELAAERERGQRNVSLVTQWAHHMKTPVAVIDLLLQRADTEEWPEEARALAAGVAEENQRLQHSLQMLLNTVRLQDFAQDFRAERLDLLALVREVINDEKRAFIARRVYPQVVAEDEGDRPWLVESDAKWLRLVLEQVVSNALKYASRPPEADEQGRVLFRLRREGGDVVLEIADNGVGIPPEDLGRVFDPFFTGLNGRAFPQATGMGLYLAREVCSQLGHGISLDSAPGEGTTVRLRFAAPQTLFAGLRDSLTANVTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL8</accession>
    <name>Q67JL8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3150"/>
    <dbReference type="EnsemblBacteria" id="BAD42132">
        <property type="protein sequence ID" value="BAD42132"/>
        <property type="gene ID" value="STH3150"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3150"/>
    <dbReference type="HOGENOM" id="CLU_3066979_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="53" mass="5881" checksum="CDC3398E094161E3" modified="2004-10-11" version="1">MSDENRPQTGAEHGHESPPVPKFLLLVYAVIAIFFVYYLAANLRFGPNSPTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM1</accession>
    <name>Q67JM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative menaquinol-cytochrome C reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH3147</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197260.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3147"/>
    <dbReference type="EnsemblBacteria" id="BAD42129">
        <property type="protein sequence ID" value="BAD42129"/>
        <property type="gene ID" value="STH3147"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3147"/>
    <dbReference type="eggNOG" id="COG1290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1098067_0_0_9"/>
    <dbReference type="OMA" id="WQANTRE"/>
    <dbReference type="OrthoDB" id="1724917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005798">
        <property type="entry name" value="Cyt_b/b6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036150">
        <property type="entry name" value="Cyt_b/b6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00032">
        <property type="entry name" value="Cytochrom_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81648">
        <property type="entry name" value="SSF81648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51003">
        <property type="entry name" value="CYTB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_CTER" evidence="11">
        <location>
            <begin position="16"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="28227" checksum="D96FFCEF6A614AA0" modified="2004-10-11" version="1">MAEAHEKYQQTGPKVKPRQKEELVYTWPFLVSIEAIMAITAILAFALMATFIDAPLLDLANPDKTPNPAKAPWYFLGLQELLLHMDPALAGVIVPGACLVLLATIPYIDRDRRGTGIWFSTKKGLPITVFSAIYTAVIEIALVLVDEVVRVPGMPEGSYRISATVTYLLEQTGLPTSAVSWVGSIFIPLVLMIGIPWVLAVIVRRRWQANTREVMIALYTFFFTSFIVLSGIGTFFRGESMHLVWPWELQPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP8</accession>
    <name>Q67JP8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Serine acetyltransferase</fullName>
            <ecNumber evidence="4 9">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH3120</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>acetyl-CoA + L-serine = CoA + O-acetyl-L-serine</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="4 9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3120"/>
    <dbReference type="EnsemblBacteria" id="BAD42102">
        <property type="protein sequence ID" value="BAD42102"/>
        <property type="gene ID" value="STH3120"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3120"/>
    <dbReference type="eggNOG" id="COG1045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_10_0_9"/>
    <dbReference type="OMA" id="GDDVMLY"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00199"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010493">
        <property type="entry name" value="Ser_AcTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06426">
        <property type="entry name" value="SATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000441">
        <property type="entry name" value="CysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SATase_N" evidence="11">
        <location>
            <begin position="5"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="25875" checksum="906C3C440BA70D14" modified="2004-10-11" version="1">MFSLFKRLRRDIEAVFERDPAARSVLEVLLCYPGLKAIWAHRVAHWLWQRNFKLLARVISQISRFFTQIEIHPGAKIGEGVFIDHGCGVVIGETAEVGNNVTIYQGVTLGGTGKEKGKRHPTIGDNVVIGTGARILGSFTVGANSRIGAGAVVLREVPPNSTVVGNPGRVVVQNGVRTDQLDMIHMPDPIANMFDQMQRQIDKLTQRIERLEEENRALRRLQEGLAHGHPHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ9</accession>
    <name>Q67JQ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197224.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3109"/>
    <dbReference type="EnsemblBacteria" id="BAD42091">
        <property type="protein sequence ID" value="BAD42091"/>
        <property type="gene ID" value="STH3109"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3109"/>
    <dbReference type="eggNOG" id="COG1811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091659_0_0_9"/>
    <dbReference type="OMA" id="IYCVGAM"/>
    <dbReference type="OrthoDB" id="1815957at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007563">
        <property type="entry name" value="DUF554"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36111">
        <property type="entry name" value="PTHR36111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04474">
        <property type="entry name" value="DUF554"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="227" mass="23573" checksum="F3DD629E38B5AE63" modified="2004-10-11" version="1">MVGLGTLANVGAVLLGGALGSLVIPQVPEEMRTSTMKALGLAVVLIGLKMAWETESILLLVVSLALGTMLGEWLRIEDRLQAWARRLERWPITQRGGFAKGFVQASLLFCTGAMAITGALEDGLNGNPSILYAKSILDGVASIMFGSVMGVGVMFAAVPVLVYQGLITLGASAISRFLTEAMIREISATGGLMVAAIGFNLMGAAEIRIGNLLPGLVVVAALAGVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR1</accession>
    <name>Q67JR1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197222.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3107"/>
    <dbReference type="EnsemblBacteria" id="BAD42089">
        <property type="protein sequence ID" value="BAD42089"/>
        <property type="gene ID" value="STH3107"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3107"/>
    <dbReference type="eggNOG" id="COG1719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_9"/>
    <dbReference type="OMA" id="NIGKRIC"/>
    <dbReference type="OrthoDB" id="1488323at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="108"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="19408" checksum="73962A2F98088508" modified="2004-10-11" version="1">MEQGRRTLPWDALGDVAAGRPHLGPMARLEICRLMQLSLCFVPEEQLGREAADRLLPEAGQAAGSAFADRLLGPMTSLSDYVRRLQATFREYGVGIVRVEEADPEQGRFVFTVEEDMDCSGLPETDRDICKFDEGFLAGLLERSMGRRYRVTEVDCWCAGARVCRFVAEAVTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS4</accession>
    <name>Q67JS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate dehydrogenase associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3094"/>
    <dbReference type="EnsemblBacteria" id="BAD42076">
        <property type="protein sequence ID" value="BAD42076"/>
        <property type="gene ID" value="STH3094"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3094"/>
    <dbReference type="eggNOG" id="COG1526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_2_0_9"/>
    <dbReference type="OMA" id="RAFWNLK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="244" mass="25720" checksum="F5566303CAE2ABD6" modified="2004-10-11" version="1">MQPPHPMPCERQTALVVNGTELLRIQTTPVDLGDWALGFLYSEAIIDGPADLLAVRVDADTCVVEAAVAPSRALHAVRAAGRYRRPDPGQGVAFDAGAHAGELRPVTHGLVVSREQVAGWMRQLQAAAPLYAATGGMHAAAVVHVPTDTLLVREDIGRHNAADKAIGAWLRSGWPPEETVLLATGRISYEMCRKAARAGIGVAASLSAATDQAIDLAEALAIDLAGYAKSPDRLVIYTAGGRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT7</accession>
    <name>Q67JT7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L7AE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197196.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3081"/>
    <dbReference type="EnsemblBacteria" id="BAD42063">
        <property type="protein sequence ID" value="BAD42063"/>
        <property type="gene ID" value="STH3081"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3081"/>
    <dbReference type="eggNOG" id="COG1358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168063_1_0_9"/>
    <dbReference type="OMA" id="KQTIRMV"/>
    <dbReference type="OrthoDB" id="2081820at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000948">
        <property type="entry name" value="Ribosomal_L7Ae_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="3">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="8732" checksum="20514AD1ED13ABB7" modified="2004-10-11" version="1">MSLERLKTASKKTIGTKQTVKAIAKGQARVVFVARDAEEHVLRGVLEAAREKGLEIEEVESMVVLGKACGIEVGAAAAAILEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K25</accession>
    <name>Q67K25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flagellar assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliO</name>
        <name evidence="6" type="ordered locus">STH2992</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197107.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2992"/>
    <dbReference type="EnsemblBacteria" id="BAD41975">
        <property type="protein sequence ID" value="BAD41975"/>
        <property type="gene ID" value="STH2992"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2992"/>
    <dbReference type="eggNOG" id="COG3190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1805187_0_0_9"/>
    <dbReference type="OrthoDB" id="1876061at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022781">
        <property type="entry name" value="Flagellar_biosynth_FliO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04347">
        <property type="entry name" value="FliO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="143" mass="15607" checksum="3C10C155DD26478C" modified="2004-10-11" version="1">MTGTETPVQAWPGGGSGSLLLFLFSALLVIALAFVTVRWLARWQFHIARGRRMRVLEGLAVGRDRYLLLVQVGQEILLLGSAEGSIRLIHRIDDPELAAEWLAESPPEQGSRPTSFAGVEGAVRANLERMRELLARKGGGPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K69</accession>
    <name>Q67K69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197062.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2946"/>
    <dbReference type="EnsemblBacteria" id="BAD41929">
        <property type="protein sequence ID" value="BAD41929"/>
        <property type="gene ID" value="STH2946"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2946"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2276045_0_0_9"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="102" mass="10697" checksum="EACB77A2E933350A" modified="2004-10-11" version="1">MQVQLIRSPKPTADGVSVEMIHSCQDMRVLLIQLSPGGEVPLATSSSSVSLQVVSGRCELVAGCEWVAAAAGTIWFYPPGEPFGVRATGEPATVLATYAPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA8</accession>
    <name>Q67KA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2907"/>
    <dbReference type="EnsemblBacteria" id="BAD41890">
        <property type="protein sequence ID" value="BAD41890"/>
        <property type="gene ID" value="STH2907"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2907"/>
    <dbReference type="eggNOG" id="COG3949">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043930_0_0_9"/>
    <dbReference type="OMA" id="PMAQVVY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038728">
        <property type="entry name" value="YkvI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37814">
        <property type="entry name" value="PTHR37814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="34490" checksum="E92F4244F8E22187" modified="2004-10-11" version="1">MVGAGFASGQETLRFFAAYGPAGLAGILLATLLFCVLGISAMNLGRRLGAETHREILHHVLGASLGRIMDGAITLFLGITLTVMIAGGGAIFAQQLGAPYALGTLLTAVVAGATILAGMRGVMAANTVVVPLLSLAVLGLVGASLHHHGLGEVLSALSPHAALAPARSWWMSALLYVSYNLVLALSVLGPLGAEIRDRTALLWGGLLGGLALGVMAAGMTLAISAHLPEAAVEIPMLYLTRFHGPFVQWFYAVVLWAEIYTTAVGCAYGFAGRLGQAWRYREAVVASICFALVGSGFGFSRLLALFYPLFGLVALVVLAGLLREPLRGVPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KG7</accession>
    <name>Q67KG7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2846"/>
    <dbReference type="EnsemblBacteria" id="BAD41831">
        <property type="protein sequence ID" value="BAD41831"/>
        <property type="gene ID" value="STH2846"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2846"/>
    <dbReference type="eggNOG" id="COG2968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1121903_0_0_9"/>
    <dbReference type="OMA" id="LPNGPHI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="DUF541"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269243" evidence="1">
        <location>
            <begin position="31"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="25739" checksum="7495004B0A915E61" modified="2004-10-11" version="1">MRNGLLTVILAGLLILGIAAFLPQAAPVHAAEDDTPVRTLSVVGRGELEVKPDTAVITVGVSEVKPTPIEAYNALSDSINKVAEALKAKGVKEEELQTSMFQLAAEYNWTQERGQELVGYRATNTLSITTQDLDKVAELIQAAVQAGANDLRGVTFQVKNADKLLDDALKLAVADAKAKAELVAGELGAKVVRVHSVSVQDQGASLVKARAGFNMDGVAAEIAPVPVYAGTSTFSATVSVTFELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI7</accession>
    <name>Q67KI7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2826</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2826"/>
    <dbReference type="EnsemblBacteria" id="BAD41811">
        <property type="protein sequence ID" value="BAD41811"/>
        <property type="gene ID" value="STH2826"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2826"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_5_2_9"/>
    <dbReference type="OMA" id="YQDTNRT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="7"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="8">
        <location>
            <begin position="329"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="825" mass="91611" checksum="3DE2153A3138429B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ0</accession>
    <name>Q67KJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2823</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2823"/>
    <dbReference type="EnsemblBacteria" id="BAD41808">
        <property type="protein sequence ID" value="BAD41808"/>
        <property type="gene ID" value="STH2823"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2823"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OMA" id="HLTACHY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="17"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="338" mass="37516" checksum="69B2F57B935B0BB5" modified="2004-10-11" version="1">MMSTAVGTKAQTGDVLVDVRNLKKHFPVTGGGIIARTVGYAKAVDGVSFQIRRGETLGLVGESGCGKTTMGKVLLRLQEPTEGQVYFEGRNIYELNREEMRKLRREMQIIFQDPYSSLNPRMTVGDIIGEPLEIHGVARGAAKTKRVHELLDVVGLASFHARRYPHEFSGGQRQRIGIARALALNPKLIVCDEPVSALDVSIQSQVLNLLEDLQKEFGLTYLFIAHGLAVVKHVSDRVGVMYLGKMMEISPANELYTNPLHPYTEALMSAIPIPDPTLKRERIVLEGDVPSPVNPPSGCRFHTRCRYQPQVGDICKEEEPPLVDVGNDHWVACHMRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV8</accession>
    <name>Q67KV8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative insertion sequence ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2703</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2703"/>
    <dbReference type="EnsemblBacteria" id="BAD41688">
        <property type="protein sequence ID" value="BAD41688"/>
        <property type="gene ID" value="STH2703"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2703"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2526300_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="2">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="84" mass="9310" checksum="421746C74B9E57E4" modified="2004-10-11" version="1">MRPSTKGSAPTCQCPPSRGRPPSNSCGDRFEKRIRVHTSPRLPIIDEQGYLTLTPVEASLLFQLVNSRYERGSISLTSTKSFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L35</accession>
    <name>Q67L35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2626</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2626"/>
    <dbReference type="EnsemblBacteria" id="BAD41611">
        <property type="protein sequence ID" value="BAD41611"/>
        <property type="gene ID" value="STH2626"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2626"/>
    <dbReference type="eggNOG" id="COG0668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_0_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="PTHR30221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="377" mass="41001" checksum="2B7273242241E4E1" modified="2004-10-11" version="1">MAWAVFAAQLLVTWAVAGLAKRILRWIAHRVVSRSDTTLDDRLVEAGTPAVRWVILALGLRLALMSLGSAIPSFQAGGRYATQFTVAGNLAEAVVVIAVTVLVNALINAALDWYLHELAGRTDGTWDRELMPMIKKGVSAVVGFFALSIVLELFGISVSALIATAGVASAAIALASQDTLSNMLGGLAILIDRPFRVGDWIELTDGKVGVVVEIGLRTTRIRQFDGTALVVPNKDMANTRVINYALPSPQAAIRQTIRVTYDTDVEKAKKVLLDVLQSHPEVLKDPVPGVWLSAFNTYSLDLFFSCWVDSYTNRFRVTDELNMQILKAFRENGIALAIPAQHIRIQQKEDSLDQAVELPAPDGNRPVQAVPGDPENH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L49</accession>
    <name>Q67L49_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4173 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2612</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196734.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2612"/>
    <dbReference type="EnsemblBacteria" id="BAD41597">
        <property type="protein sequence ID" value="BAD41597"/>
        <property type="gene ID" value="STH2612"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2612"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025121_0_0_9"/>
    <dbReference type="OrthoDB" id="1213947at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025231">
        <property type="entry name" value="DUF4173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13777">
        <property type="entry name" value="DUF4173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4173" evidence="2">
        <location>
            <begin position="271"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="512" mass="54906" checksum="02C9B1CE0FBB38AC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L54</accession>
    <name>Q67L54_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196729.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2607"/>
    <dbReference type="EnsemblBacteria" id="BAD41592">
        <property type="protein sequence ID" value="BAD41592"/>
        <property type="gene ID" value="STH2607"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2607"/>
    <dbReference type="eggNOG" id="COG2968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080344_4_0_9"/>
    <dbReference type="OMA" id="IRLQPNY"/>
    <dbReference type="OrthoDB" id="1825422at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="DUF541"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="227" mass="23450" checksum="487240F953613509" modified="2004-10-11" version="1">MGTFMGYPAGFAAGPVEPPTLQVTGVGEAGAAPDTARLTLGFTARAPQAAAAYEQTAAALNRVVQALMQAGLAADQMQTQEVALNPVFDRPRDGGETRLTGYEATATLAVTLRDLARVGAIIDLAVSAGANRVDGVQFTVRERERAEAAALTQAVQDAQRQAAVLARALGVALGPVREVSTEAAPGPSPYLARSAAEGIPVLPGRLAVVRTVRLAYQIHPSVTARAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L66</accession>
    <name>Q67L66_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative fosmidmycin resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2595</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2595"/>
    <dbReference type="EnsemblBacteria" id="BAD41580">
        <property type="protein sequence ID" value="BAD41580"/>
        <property type="gene ID" value="STH2595"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2595"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040537_0_0_9"/>
    <dbReference type="OMA" id="YFYMASF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="14"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="393" mass="41047" checksum="3A25010AB5144F3B" modified="2004-10-11" version="1">MNSTVSAGRPVLALLLVLSLTHAVIDLTSGAIVALLPAMREAYSLSYAMVGLIPLFANLTSTFTQPVFGIISDRSEMRWLLPLSVALGGLGLAAVGYVSNYWLVLAAVVISALGSAAFHPEGAHAAHNLAGERRALAMAIYSVGGNLGYALGPGFAAALIAAFGLRGTGLAALLPLALAAFLIWLLPRWRAHEQEAAARKREQAEPGETNWRGTVLITLVVILRSIIHLGMTTYVPFFWIDVLGNAPATAGLVQTTYLLAGVLGTLLGAPLADRFGTKPVLIASFALLLPVQAVLASLRGWPLLIGLFAAGFLVVSTFTTTLVMTQEYMPRSLGLASGLNLGLAFGMGGVGTSVLGIVADHFGVLVVMRCIASLVLPALLLTLALPPVRLNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA0</accession>
    <name>Q67LA0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196683.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2561"/>
    <dbReference type="EnsemblBacteria" id="BAD41546">
        <property type="protein sequence ID" value="BAD41546"/>
        <property type="gene ID" value="STH2561"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2561"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_6_2_9"/>
    <dbReference type="OMA" id="ICAQACM"/>
    <dbReference type="OrthoDB" id="1814691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="56"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="17120" checksum="1D98BB4C2C37EAD7" modified="2004-10-11" version="1">MSEPFVSERDHLQGLHWQCFGCGQENRLGLRLNIRLVGDRTECTFTPIPEFQGPPGVAHSGIIATVLDEVMSQLIYAQLQLAVTRKIEITYRRPIRVGQTYYFSAWIVKQNTRIIAAKAEARDAEGNQVARARGIFAPMDEERVAKFLDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC7</accession>
    <name>Q67LC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Malate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196657.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2534"/>
    <dbReference type="EnsemblBacteria" id="BAD41519">
        <property type="protein sequence ID" value="BAD41519"/>
        <property type="gene ID" value="STH2534"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2534"/>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034446_2_1_9"/>
    <dbReference type="OMA" id="HKYAAVV"/>
    <dbReference type="OrthoDB" id="654531at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="6">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="5">
        <location>
            <begin position="176"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="43905" checksum="519BF985DF85F693" modified="2004-10-11" version="1">MENRTGDGRQNPARNEADLKEAALRLHVQNRGKWTTASKVPLQTQEDLALAYSPGVAEPCRAIAADPEAVFRYTARGNLVAVVSDGSAVLGLGDIGPEAALPVMEGKALLFKAFADIDAVPLVLAERDVDEIVRTVTRLSPTFGGINLEDIAAPRCFAVERKLQAAVDIPVFHDDQHGTAIVVAAGLQNALKLVGKRLEEVRVVIEGAGASGLATAALLLDLGVSDLTLVDSRGTIYPGRPDGMNPFKEELAQRTNPRRVRGGLAEALAGADVAVGLARGGSMTREMVRAMAPDPIVFALANPEPAIWPEEAKAAGAAVVATGRSDYPNQLNNVLAFPGIFRGALDVRAREINEAMKRAAVRAIADLVAPEELSADYIIPAPLDRRVAPAVAAAVAEAAVRSGVARSPGFDSFDPYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH7</accession>
    <name>Q67LH7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenylacetic acid degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaC</name>
        <name evidence="1" type="ordered locus">STH2484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2484"/>
    <dbReference type="EnsemblBacteria" id="BAD41469">
        <property type="protein sequence ID" value="BAD41469"/>
        <property type="gene ID" value="STH2484"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2484"/>
    <dbReference type="eggNOG" id="COG3396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070585_1_0_9"/>
    <dbReference type="OMA" id="NGDFAHT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011882">
        <property type="entry name" value="PaaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037834">
        <property type="entry name" value="PA_CoA_Oase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02158">
        <property type="entry name" value="PA_CoA_Oxy3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28272" checksum="C65B82E10EC796CC" modified="2004-10-11" version="1">MIALLYQLADDELVIGHRDSEWLGLAPHIEEDVAFSSIAQDEVGHAALFFRLLEELGQGRADDLAFLRPAGRRRNAVLLERPNGPGTYLDEPRFDWAYTVVRRLAYDLFDAIRLEVLCSSSYAPLAQAAAKVRREERYHLLHHTTWFRRLATGTDESRRRLEAAVAAVWPDVGGLFTPGEVAPGPHFPVRPDELAGRWAERMAPLFAEVGLTWPGRPESAAAPGADGRLGQHTPDLEGLLSTMGEVYRTAPGAAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL6</accession>
    <name>Q67LL6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Signal peptidase I</fullName>
            <ecNumber evidence="4 7">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196568.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2445"/>
    <dbReference type="EnsemblBacteria" id="BAD41430">
        <property type="protein sequence ID" value="BAD41430"/>
        <property type="gene ID" value="STH2445"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2445"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_5_1_9"/>
    <dbReference type="OMA" id="RSQVWVK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="8">
        <location>
            <begin position="17"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="20368" checksum="D09292ACC2AF41DB" modified="2004-10-11" version="1">MAPPQEGPAPARRGLVREILETAALALVVALVVRTFGVQVFRVEGESMLPTLAHGDRLLVNKLVYRLREPAPGEVVVIADPANPHRHLVKRVIAVAGDEVAVEGDAVWVNGRLLDEPYVHPGSPGTYRAGPLTVPEGYVWVMGDNRGASLDSRLLGPIPVARVEGRAAALVWPPVRIGDHGPLAAARTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS4</accession>
    <name>Q67LS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196510.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2387"/>
    <dbReference type="EnsemblBacteria" id="BAD41372">
        <property type="protein sequence ID" value="BAD41372"/>
        <property type="gene ID" value="STH2387"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2387"/>
    <dbReference type="eggNOG" id="ENOG5032ZSJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_198029_1_0_9"/>
    <dbReference type="OMA" id="CWDCCVE"/>
    <dbReference type="OrthoDB" id="2038589at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7586" checksum="70CC8A0B319D657C" modified="2004-10-11" version="1">MRCPNCGSRSIGRVGAEQYYCWDCCVEFNTARGAVRIYHLDSDGELIAAAEHRVEASAPLGPTNERRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG3</accession>
    <name>Q67MG3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD-GYP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2145</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2145"/>
    <dbReference type="EnsemblBacteria" id="BAD41130">
        <property type="protein sequence ID" value="BAD41130"/>
        <property type="gene ID" value="STH2145"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2145"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_1_9"/>
    <dbReference type="OMA" id="MPHEACE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="144"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="44923" checksum="8EC18D843554543A" modified="2004-10-11" version="1">MRNRGGGPCILRDAALICPSNPEVYTLYRVPTTDIRAGMRLGKTIFSANGQVLLQAGTVLRESFIGPLLSYGITTVYIVNELAPDVEPLDVVSDETRIQLKDQMQRTMEEVRAALAEATHAGLRHFSPTIRTDGLKQTIDQIIAELLSNPKVAVSLVDIRSADQYTLSHSVNVCILSTLLGIELGFSLDDLRDLALGTLLHDIGKVAIPPEILNKPDRLTPEEAEIMKRHTVLGWELLKNQPEIPPTAAVVALQHHERWAGGGYPHNLRDNQIYRYARVCAIVDCYDAMTSDRIYRKGLSPAMALESLSGPMHGFFDPHFLLAFLNCIAPWPVGSMVELTGGRKAVVVAAERGRADRPRVRVLLEPDGSPTPQPYEIDLFRERHVEIIREVHEDADQYMREMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI2</accession>
    <name>Q67MI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196251.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2126"/>
    <dbReference type="EnsemblBacteria" id="BAD41111">
        <property type="protein sequence ID" value="BAD41111"/>
        <property type="gene ID" value="STH2126"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2126"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924161_0_0_9"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="301" mass="32869" checksum="F138F6DD0ED86FC6" modified="2004-10-11" version="1">MKTYIALVRHGVTDWNYDGRAQGQVDIPLNAEGERQAGAVAARLATERWDAVYSSDLARARATAEAICRLTGHALITDERLRERSMGPAEGMTAVEREALWPGVALSDIPGVESDAALGLRAEAVLTEIARRHPGQRIVVVSHGALLNRFLQQTVGTKPVNFRNTGISPVVWDGQGFRPAGQHDYRHLLVDGVEYDGVRTRLAHHVQREGLPGVALPPEKAALMVRNASAVDAAWVDDRLVGYVRAFTDRVRYGYIDLLYTAPGYERVGAVLQRRIAERYPEVRFELIAGGAGETGEATGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N00</accession>
    <name>Q67N00_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sigK</name>
        <name evidence="9" type="ordered locus">STH1958</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the sigma-70 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1958"/>
    <dbReference type="EnsemblBacteria" id="BAD40943">
        <property type="protein sequence ID" value="BAD40943"/>
        <property type="gene ID" value="STH1958"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1958"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_7_9"/>
    <dbReference type="OMA" id="LMYLRQN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014209">
        <property type="entry name" value="RNA_pol_sigma-K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02846">
        <property type="entry name" value="spore_sigmaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="8">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="236" mass="26306" checksum="3AB016FABFE41066" modified="2004-10-11" version="1">MLAMLGPLAEAIVRSMLLLAGFVANSGTFPQPLSEEEEAKYLSRLQNGDEEARAVLIERNLRLVAHIVKKFDNTGEDVDDLISIGTVGLIKAIGTFKPERGTRLATYAARCIENEILMHLRSLKRVRGEVSLYDPIGVDREGNEITLIDVLGSDPDVVPDLVGKRLDESKLREKLKKLGGKERQVLELRYGIAGGPRKTQREIARMLGISRSYVSRIEKKAVMKLCRELFEEGYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N04</accession>
    <name>Q67N04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196081.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1954"/>
    <dbReference type="EnsemblBacteria" id="BAD40939">
        <property type="protein sequence ID" value="BAD40939"/>
        <property type="gene ID" value="STH1954"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1954"/>
    <dbReference type="HOGENOM" id="CLU_782862_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="354" mass="38308" checksum="2AA1237C501BC8D4" modified="2004-10-11" version="1">MDVTAAFDPLNPSVEAISLRQRVNFLATAADHAYGRLLELFPEAASAGRPQIRLYESHEAFRAAVGAAAPPDALAWYNPGDPLRLSPEFLRGLMRWETERDLGYEFVKHISAAASGQPVALIDPIAMGLFERSTAGDLPYLPDPRRLVGTPLPDLAALFSTPVQSLGAAGQRAYATAAAELVRFLQDRLPAEELQGPAPGRGWSLGALADRLGQTPETLAAEFEVFLHRQLQATSVLNVPAAQSRVPEGLPDAIARRAEAAAGGDVEAFLRRTSPAHRDGWSAWLAAARRYGLVRYEASLLDWERNEGVALVLERLQFRDGRTVIGVVRQHWALEDAGWAAGPVESVWTGADGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N25</accession>
    <name>Q67N25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1933</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196060.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1933"/>
    <dbReference type="EnsemblBacteria" id="BAD40918">
        <property type="protein sequence ID" value="BAD40918"/>
        <property type="gene ID" value="STH1933"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1933"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_9"/>
    <dbReference type="OMA" id="EVINPEM"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="29311" checksum="C13125DCC1128C6A" modified="2004-10-11" version="1">MLRIESLAVGYGGEPVLNGLDLAVARGEFLVVVGPNGSGKSTLVRAVTGALPPTEGRILLDGRDLRGLRPREVARTLAVVAQDTSVGFPFTVEEIVALGRIPHLPPLRSETPRDRAAVERAMRLTGTQPLAGRLVTALSGGERQRVMVARALAQEPRLLILDEPTAHLDIAHQVGLLDLVRRLNRTEGLTVLAILHDLNLAALYADRLVMLRGGRVWADGPPAEVLTEANILTVYGSRVRIVRHPSAGQPQVMLLSREVAAPAQPLEPVSAGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N84</accession>
    <name>Q67N84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1874</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1874"/>
    <dbReference type="EnsemblBacteria" id="BAD40859">
        <property type="protein sequence ID" value="BAD40859"/>
        <property type="gene ID" value="STH1874"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1874"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481386_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="3">
        <location>
            <begin position="253"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="566" mass="58255" checksum="0C6EDCBC4D2507C1" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N96</accession>
    <name>Q67N96_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stage III sporulation protein AA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spoIIIAA</name>
        <name evidence="4" type="ordered locus">STH1862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1862"/>
    <dbReference type="EnsemblBacteria" id="BAD40847">
        <property type="protein sequence ID" value="BAD40847"/>
        <property type="gene ID" value="STH1862"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1862"/>
    <dbReference type="eggNOG" id="COG3854">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052793_0_0_9"/>
    <dbReference type="OMA" id="ISPPGCG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014217">
        <property type="entry name" value="Spore_III_AA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045735">
        <property type="entry name" value="Spore_III_AA_AAA+_ATPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19568">
        <property type="entry name" value="Spore_III_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02858">
        <property type="entry name" value="spore_III_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="126"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="292" mass="30928" checksum="D5E84953578007A6" modified="2004-10-11" version="1">MPAPPEEIRIRQGRPLHLVYPGGDAFVTPDGRLTADPARAVRPDGDDLQRTFQLMAKGSVYAWEEEVRGGFLTLEGGHRVGLCGRAVTEACRIRTLKQVASLNVRIAREVPGAADGLLPHLVRGGRFLSTLLVSPPQAGKTTLLRDLVRQLSTGVPRLGLRGLKVGLVDERSEVAGCVCGVPQRDVGPRTDVLDGCPKAEGLMLMIRALSPQVVAADEIGRPEDAVAVMEALHAGVAVLATAHGAGLSDVRRRPALAELLRAGAFERAVVLGRSRGPGTVEAVLDLAREVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND3</accession>
    <name>Q67ND3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1825</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195953.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1825"/>
    <dbReference type="EnsemblBacteria" id="BAD40810">
        <property type="protein sequence ID" value="BAD40810"/>
        <property type="gene ID" value="STH1825"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1825"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_5_2_9"/>
    <dbReference type="OMA" id="DYQVHPG"/>
    <dbReference type="OrthoDB" id="1831818at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="19271" checksum="D76323BC8A6F3305" modified="2004-10-11" version="1">MRVIRQEEIYRGRVIAVRRDLIDLDGKERTWDVVAHPGAVVVLPVDGDDLLMVRQYRYAAGETLLELVAGGLEPGEDPAEAAQRELQEEAGFRAGRLIRLAEFYSAPGFCTEKLHLFAAEELTPSRLPMDEDEQIELVRLSLDEALRMALAGELRDAKTLAGVLLYARLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG6</accession>
    <name>Q67NG6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1792"/>
    <dbReference type="EnsemblBacteria" id="BAD40777">
        <property type="protein sequence ID" value="BAD40777"/>
        <property type="gene ID" value="STH1792"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1792"/>
    <dbReference type="eggNOG" id="ENOG5032X8M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_852226_0_0_9"/>
    <dbReference type="OMA" id="MAMISSC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="326" mass="34734" checksum="42E5F0E783D994AB" modified="2004-10-11" version="1">MDERLRRLAERYRAGNGDRRPVKERVAALYRTYVAKREGALIDAAAAAAAVHSLFAADPASAVAVDPRLEEAFRLAFPNVDPESLRGLDPDALAPYLPALKGKYFEVLVRDRLNEGQWVGDIRLAPGQRAVLADSATQPGWDLQILNEDGSVAELLQLKATNSLSYVKAALERYPDIQVLTTDEVLAAGGDLQGVLGSGFSDAELEQAVDRGIEALFGDASESVLGEVLPALPFVLIALREGRCVLMGRKTLAVACQDGLTGAARSGVAIGAGALAANLVSPGFGMATAALVRMGIHRHDRLSRASGLIQVRLEQLKRLRARPAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH7</accession>
    <name>Q67NH7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1781</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195909.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1781"/>
    <dbReference type="EnsemblBacteria" id="BAD40766">
        <property type="protein sequence ID" value="BAD40766"/>
        <property type="gene ID" value="STH1781"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1781"/>
    <dbReference type="HOGENOM" id="CLU_2107754_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="13390" checksum="84E226432F386CA0" modified="2004-10-11" version="1">MAYTLQTFIHHKVFGNHLSKCKPLTYRKEDWLHLTRGRERSVRLIIRVMLGVPSAHHGPNEHAMWCFQFPKGSLLTVHLHRGTVAEISTYEADKDELEEAVDYLLEEVAARLRQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL0</accession>
    <name>MUTL_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutL</name>
        <name type="ordered locus">STH1748</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195876.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NL0"/>
    <dbReference type="SMR" id="Q67NL0"/>
    <dbReference type="STRING" id="292459.STH1748"/>
    <dbReference type="EnsemblBacteria" id="BAD40733">
        <property type="protein sequence ID" value="BAD40733"/>
        <property type="gene ID" value="STH1748"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1748"/>
    <dbReference type="eggNOG" id="COG0323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_4_1_9"/>
    <dbReference type="OMA" id="AHERIMY"/>
    <dbReference type="OrthoDB" id="764332at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000096694" description="DNA mismatch repair protein MutL">
        <location>
            <begin position="1"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="635" mass="68299" checksum="8DC896BB5A9FF85B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL4</accession>
    <name>Q67NL4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcription elongation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1744"/>
    <dbReference type="EnsemblBacteria" id="BAD40729">
        <property type="protein sequence ID" value="BAD40729"/>
        <property type="gene ID" value="STH1744"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1744"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_5_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00830">
        <property type="entry name" value="GREAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GreA_GreB" evidence="1">
        <location>
            <begin position="75"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16891" checksum="7ED3C4E7285F4E19" modified="2004-10-11" version="1">MVRARPPSEIEHMRSRIASRLRVLEELLNTQMEEYFSSPGSEGLAYSVRPHLRRYVEVAGQLLERMGGPDGDPVDDVVFMDVPVTLVDETDGTEEVYTVVLPEEGDPAKGHISCLSPLGLALLLKRVNDRVVIDAPGGRFTYRIAAVGSLAASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN7</accession>
    <name>Q67NN7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell division protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ftsA1</name>
        <name evidence="2" type="ordered locus">STH1721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195849.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1721"/>
    <dbReference type="EnsemblBacteria" id="BAD40706">
        <property type="protein sequence ID" value="BAD40706"/>
        <property type="gene ID" value="STH1721"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1721"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010661_0_0_9"/>
    <dbReference type="OMA" id="MVPMAGD"/>
    <dbReference type="OrthoDB" id="1044637at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="717" mass="75318" checksum="48591A33C05FEC88" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP2</accession>
    <name>Q67NP2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="4">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195844.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1716"/>
    <dbReference type="EnsemblBacteria" id="BAD40701">
        <property type="protein sequence ID" value="BAD40701"/>
        <property type="gene ID" value="STH1716"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1716"/>
    <dbReference type="eggNOG" id="COG1183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_2_0_9"/>
    <dbReference type="OMA" id="PVLWLRQ"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="29989" checksum="4844E2D2CD011D29" modified="2004-10-11" version="1">MNENTVMQPHRPRWRVRTVRGGKWIRERGQKGLSSLPHLFTLANLSFGIGSLLLTMDGQWRAASLMVVGSLVADGLDGRLARLMKAEGAFGRELDSLADVVAFGVAPAVLLYQMGLRELGYLGLAVTLFFPLCGALRLARFNILTNEGYFLGVPITAAGTLLSTMAFYFLQETTAPPPPHVIPSVMVILGYLMTSSIPYPDFKKRRKGAGIQFWPIVGPVLALAALLFAAGWNPWAVILMPLTAYVVLGPWLVVLRKWDEKVQPWLADVTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ4</accession>
    <name>Q67NQ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1704</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1704"/>
    <dbReference type="EnsemblBacteria" id="BAD40689">
        <property type="protein sequence ID" value="BAD40689"/>
        <property type="gene ID" value="STH1704"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1704"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="AEQVYIE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="37"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="69"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="271" mass="29638" checksum="81FA1E1D55633859" modified="2004-10-11" version="1">MTGDGRRLATPRRPGAVALRPSARPGGGQRRMTEALLVLENIRKRFGQREVLHDVSLTVRRGERVALLGHNGAGKSTLLRVATGLLAADGGLAIIGGLPAAAGHREAKMLVGYLPDVPPLYEALTPAEYLEYVAALWNIPSRVATERAAGYLKQYQLWEARHTWIQNLSKGMRQKVGLISVFLREPPLLLLDEPFSGLDAEAAELTVQLLRTWGDDRAVIVASHDVELVERLAERAVVLRQGRMVADFPVRRGRLATDLRQFAQGAAEVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR1</accession>
    <name>Q67NR1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="4">3.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="4">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ahcY</name>
        <name evidence="8" type="ordered locus">STH1697</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>H2O + S-adenosyl-L-homocysteine = adenosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.3.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="4 5">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 6">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.3.1.1" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195825.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1697"/>
    <dbReference type="EnsemblBacteria" id="BAD40682">
        <property type="protein sequence ID" value="BAD40682"/>
        <property type="gene ID" value="STH1697"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1697"/>
    <dbReference type="eggNOG" id="COG0499">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_0_2_9"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="OrthoDB" id="522981at2"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AdoHcyase_NAD" evidence="7">
        <location>
            <begin position="190"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="219"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="298"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="45884" checksum="1B7FDDC600A05D07" modified="2004-10-11" version="1">MPSKVSVSTLRDPGLAPSGHQKIDWVKAHMPILNQLERELSAHRPLAGQRVAMSIHLEAKTAYMALVFAAAGAEVFLTGSNPLSTQDDVAAAAAERGVTVHAWHGATPEEYTAHLTRTLEAARPTLLLDDGGDLTHLLHTGRADLAANLIGGSEETSTGVQRLRAMEAEGVLRFPMVAVNNARMKHLFDNRYGTGQSTLESVMRNTNLSIAGKRVVVAGYGWCGKGVAMRAKGLGARVIVCEVDPVLANEALMDGFEVMPMARAAALGDIFITVTGCEKVIRREHFEVMRDGAILANAGHFDVEIWKPDLEAFGGQPVRVRPHVDAYTAPDGRRLYLIGEGRLANLAAGDGHPAEVMDLSFGVQLLTHLWLVENRGRLENRVIEVPPEIDTRVAETRLRALGVEIDRLTPEQERYIRSWQV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR4</accession>
    <name>NDK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">STH1694</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195822.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NR4"/>
    <dbReference type="SMR" id="Q67NR4"/>
    <dbReference type="STRING" id="292459.STH1694"/>
    <dbReference type="EnsemblBacteria" id="BAD40679">
        <property type="protein sequence ID" value="BAD40679"/>
        <property type="gene ID" value="STH1694"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1694"/>
    <dbReference type="eggNOG" id="COG0105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_9"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="OrthoDB" id="1752581at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137060" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="150" mass="16797" checksum="7167707257696A00" modified="2004-10-11" version="1">MERSFVMVKPDGVQRGLIGEVISRLERRGLKLVAAKLMRVSRELAEEHYAQLKDKPFFPSLIEFITSGPVMAMVWEGPNAISIIRKTMGATNPANAEPGTIRADFACDVSYNVVHGSDGPESAEREIRLWFGEVEPSYSRSVDRWIFPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P00</accession>
    <name>Q67P00_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195737.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1608"/>
    <dbReference type="EnsemblBacteria" id="BAD40593">
        <property type="protein sequence ID" value="BAD40593"/>
        <property type="gene ID" value="STH1608"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1608"/>
    <dbReference type="HOGENOM" id="CLU_2453521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="89" mass="10305" checksum="4CCBFE87900DB39D" modified="2004-10-11" version="1">MNYLVVIVLALTAVVVVSVIRTRRDRELLADEVRRRGGEVIRLIRARRGSPFPDTGRGWWAWKVEWRDAGGERTSWALTTRDGLGEWRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P21</accession>
    <name>NUOB1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit B 1</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit B 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit B 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoB1</name>
        <name type="ordered locus">STH1587</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD40572" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40572.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_043713797.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P21"/>
    <dbReference type="SMR" id="Q67P21"/>
    <dbReference type="STRING" id="292459.STH1587"/>
    <dbReference type="EnsemblBacteria" id="BAD40572">
        <property type="protein sequence ID" value="BAD40572"/>
        <property type="gene ID" value="STH1587"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1587"/>
    <dbReference type="eggNOG" id="COG0377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_9"/>
    <dbReference type="OMA" id="GWARKYS"/>
    <dbReference type="OrthoDB" id="1904620at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000358486" description="NADH-quinone oxidoreductase subunit B 1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="166" mass="18213" checksum="A0080192E6A41E9A" modified="2008-12-16" version="2">MSADGVFRDQGGFVTTTVDSFLRWAQSNSIWPLTFGLACCAIEMMNLASGPRYDIARFGSEAFRASPRQADLIFISGRVSNKMAPVIKRVYSQMLEPKWVVAFGACASSGGIFDNYAIMQGVDNLLPVDIYVPGCPPTPEAVIYAVQKLRDRIRKEDPRGGIIVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P44</accession>
    <name>Q67P44_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 4-hydroxybenzoate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195693.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1564"/>
    <dbReference type="EnsemblBacteria" id="BAD40549">
        <property type="protein sequence ID" value="BAD40549"/>
        <property type="gene ID" value="STH1564"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1564"/>
    <dbReference type="eggNOG" id="COG0043">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_4_1_9"/>
    <dbReference type="OMA" id="SYQQFWG"/>
    <dbReference type="OrthoDB" id="1012819at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022390">
        <property type="entry name" value="HBDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03701">
        <property type="entry name" value="mena_SCO4490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="484" mass="54285" checksum="E5C8422F6BE3C6E4" modified="2004-10-11" version="1">MAFRDLHEFIAACEQRGWLKRIKAPVSPYLEIAEITDRVSKMPGGGPALLFENVEGSRFPVLTNTLGSMERICLALGVNHLDEIGQRIRDLLKLPTSGGGLLDKLGQGMKLLDVARSTAPRLVNRAPCQEVVLTGDDVDLYKLPILTTWPKDGGPFITLTNVITRDPVTGGRNIGMYRMQVYDRRTTGMHWHKHKDGQRHFDAYQGTRMPVAVALGGDPATIYSGSAPLPPVIPELMFAGFLRGEPVELVKCKTIDLEVPAHADFILEGYVDTAEPLRPEGPFGDHTGFYSPVDPYPVFHVTAITHRRDAIYPATVVGKPPQEDAYLGKATERIFLPLLQLFMPEIIDYNMPVEGGFHNCVLVKIKKRYPGHARKVVHGLWGLGLMMLSKCIIVVDEHIDLNNYSEVFWYVSGNIDPKRDIFFAEGPVDDLDHAAPVWRYGSKMGIDATRKWPEEGHPRPWPEEIEMSPEVKERVTARWKEYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P63</accession>
    <name>Q67P63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dipicolinate synthase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195675.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1545"/>
    <dbReference type="EnsemblBacteria" id="BAD40530">
        <property type="protein sequence ID" value="BAD40530"/>
        <property type="gene ID" value="STH1545"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1545"/>
    <dbReference type="eggNOG" id="COG0169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082687_0_0_9"/>
    <dbReference type="OMA" id="MMAIQHT"/>
    <dbReference type="OrthoDB" id="1871452at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014215">
        <property type="entry name" value="Dipicolinic_acid_synth_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031629">
        <property type="entry name" value="DpaA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16924">
        <property type="entry name" value="DpaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02853">
        <property type="entry name" value="spore_dpaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DpaA_N" evidence="2">
        <location>
            <begin position="8"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="1">
        <location>
            <begin position="146"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="31753" checksum="5118C1BEE1BFAD37" modified="2004-10-11" version="1">MSVYGKIIAVIGGDRRMAEAVHFLLRAGARVRIAGLEWEDRFAGVEVCTSGADALTGAQVVILPVQGVSPDGTVYTEGDVPPCVIDLDGLRRVARGAPVFVGLSNPHLNRLCEEAGLPLIEYREADHFATWNSIPSAEGAIQMAMESTPFTIFGSRSLVLGFGRTGRAIAMLLRGLMSDVSVAARSELDQARIWAAGHRPVEWPKLADAVAEADIIFNTVPALVLTRDILSRAPRHAVIIDVASAPGGTDFEAARELGLTARLAPGLPGIVAPVTAGRIIAELVVRHLRRTDAMEGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P73</accession>
    <name>Q67P73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheW2</name>
        <name evidence="2" type="ordered locus">STH1535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195665.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1535"/>
    <dbReference type="EnsemblBacteria" id="BAD40520">
        <property type="protein sequence ID" value="BAD40520"/>
        <property type="gene ID" value="STH1535"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1535"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_1_9"/>
    <dbReference type="OMA" id="MPQYVEG"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="16183" checksum="F51D6E311F2A6812" modified="2004-10-11" version="1">MRRGTAAEQKVVVFAIGGQEYALPIAGVREIITWTAPTPVPDSPPYVEGVISVRGEVLPVMDLGRRFGLTSRREAEQRRIIILELAGKSAGLIVDDVVAVEDVVEGKLAPPSPLVANLGGMHEAVVEGILQLGEGRLVVVLSADRLLETALQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG0</accession>
    <name>FABH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="1">2.3.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier-protein] synthase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-ketoacyl-ACP synthase III</fullName>
            <shortName evidence="1">KAS III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabH</name>
        <name type="ordered locus">STH1448</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="EC" id="2.3.1.180"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. FabH family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.180" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195578.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PG0"/>
    <dbReference type="SMR" id="Q67PG0"/>
    <dbReference type="STRING" id="292459.STH1448"/>
    <dbReference type="EnsemblBacteria" id="BAD40433">
        <property type="protein sequence ID" value="BAD40433"/>
        <property type="gene ID" value="STH1448"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1448"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_3_1_9"/>
    <dbReference type="OMA" id="YAKHKGK"/>
    <dbReference type="OrthoDB" id="872193at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01815">
        <property type="entry name" value="FabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004655">
        <property type="entry name" value="FabH_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00747">
        <property type="entry name" value="fabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000187908" description="3-oxoacyl-[acyl-carrier-protein] synthase 3">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="ACP-binding" evidence="1">
        <location>
            <begin position="256"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01815"/>
        </source>
    </evidence>
    <sequence length="330" mass="34934" checksum="551CC24715B9191A" modified="2004-10-11" version="1">MKIRPVGITGLGMAVPERVLTNHDLERMVDTSDEWIRTRTGIRERRIAAPHEASSDYALRAAREAMAQAGVTPEQIDLIICATVTPDMPMPATAALVQAALGAHRAAAFDLSAACPGWIYGVVMAQQSIATGLYDCALVIGVELLSKMVNWQDRKTCVLFGDAAGAAVLQPVSEGRGILSSVLGAEGAGHCHLYTPAGGSRLPASPETVAQGLHYVHMNGPEVFKFAVRVMDEATVQVVEKAGLTVGDIDLLVPHQANVRIIDSAVKRLGLAPEKVVVNLDRYGNTSSASIPVALTEALTEGRVRDGDLVVCVSFGAGLVWGALALRWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL1</accession>
    <name>Q67PL1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1397"/>
    <dbReference type="EnsemblBacteria" id="BAD40382">
        <property type="protein sequence ID" value="BAD40382"/>
        <property type="gene ID" value="STH1397"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1397"/>
    <dbReference type="HOGENOM" id="CLU_933601_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="31146" checksum="DFE9E36DF36998AB" modified="2004-10-11" version="1">MTARRRTESPAPPRKEAGGRRPGWWSPPCRPPSGSGGTAPQPWSARMRRRRRVGMRADRPQHPGDPGRSPAVEQLAAQLDRVIQALGRLVLVLEELTARPPVHLQVERLEVQTLAFHLGDIDVEQLEGQLNIGITHSLTLESGPSPGEDEPVRPPGAPRARPFEVDPARPAPFGAGTPGRRAAGESAPDGRAGGKPGPGDDDSGRPPRALRNSVVSRWLAQPGQGAPARSGRPAGDRPEQAGPACETAPAGTHPEAAGPADAGPAAADGSSPPRIQIWPPPDAEGSETDGEGQPGAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM5</accession>
    <name>Q67PM5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1648 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195513.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1383"/>
    <dbReference type="EnsemblBacteria" id="BAD40368">
        <property type="protein sequence ID" value="BAD40368"/>
        <property type="gene ID" value="STH1383"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1383"/>
    <dbReference type="eggNOG" id="COG5658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093038_0_0_9"/>
    <dbReference type="OMA" id="VGIKTPW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012867">
        <property type="entry name" value="DUF1648"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026272">
        <property type="entry name" value="SdpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025962">
        <property type="entry name" value="SdpI/YhfL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37810:SF5">
        <property type="entry name" value="PTHR37810:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07853">
        <property type="entry name" value="DUF1648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13630">
        <property type="entry name" value="SdpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038959">
        <property type="entry name" value="SdpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1648" evidence="2">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="25215" checksum="375358B31CB18F92" modified="2004-10-11" version="1">MAMKEFTYRVDRLTVAGDWPFFAFLLATIAVTAWAFPRLPDQAAVHFDAAGTADRFAGPWFLALWWPIFATGLYLLLLVLPLADPRRANYASFGRIYGYLRQATPAFLLVIHLAQLADALGMPISPLSVALVAMSALFLLLGLVMDRFRYNYFVGFRTPWTLASEEVWNRTHAFGSRVWVALSLLNALVVLLPLHLQLWASMGVIAVMVVVPVVYSHLVFRQVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS5</accession>
    <name>Q67PS5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dioxygenase related to 2-nitropropane dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195464.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1333"/>
    <dbReference type="EnsemblBacteria" id="BAD40318">
        <property type="protein sequence ID" value="BAD40318"/>
        <property type="gene ID" value="STH1333"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1333"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_0_0_9"/>
    <dbReference type="OMA" id="ATYECDA"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="80"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="333" mass="35251" checksum="C49125C5BBE8A702" modified="2004-10-11" version="1">MNLPSLQVGHKTARYCIVQGGMSVRIALHRLAAAVAECGGVGTIGGMGIPPGELRREIRAAKALTDGVVGVNLMYAGYLFDRLLDVCIEERVDYVAIGAGFARGPFKKLHEAGIPGFCIISSVKAARIVARTPGVAGVVVESGQAGGHLGPEDPEISTWELFPPVRAALREAGFAGPIIAAGGLLTRQDVLRALAMGADGVQMGIRFAMTEESAASAEMKARWLAATGSQVTDWSPTGYKSRAIVPHIPFEALPRTRENGGTYSCTNCLKHCLHRDEGRSHCIWNALSNAQKGRVENGLIFCGGRVGEIHDIVPVAEVFRRLTTPSADEAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX5</accession>
    <name>Q67PX5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron-siderophore uptake ABC system ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1283</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1283"/>
    <dbReference type="EnsemblBacteria" id="BAD40268">
        <property type="protein sequence ID" value="BAD40268"/>
        <property type="gene ID" value="STH1283"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1283"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035418_0_0_9"/>
    <dbReference type="OMA" id="VYQNPFS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="287"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="319"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="562" mass="60357" checksum="D64DA5F749A85C07" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY8</accession>
    <name>Q67PY8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">M20_dimer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1270"/>
    <dbReference type="EnsemblBacteria" id="BAD40255">
        <property type="protein sequence ID" value="BAD40255"/>
        <property type="gene ID" value="STH1270"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1270"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029469_2_1_9"/>
    <dbReference type="OMA" id="RGNCYLE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="191"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="459" mass="49840" checksum="86331F45645EC1A2" modified="2004-10-11" version="1">MGYLEEHQSRFLNEFLSLLRIPSISTDPAAAGAVRQAAEWVAERLRQAGMDGVRILETGGHPAVYGQWCGAPGRPTVLIYGHFDVQPVDPLHLWTHPPFEPAVVDGRVYARGATDMKGNLLLPIVACEALLRTEGQLPVNVKFLLEGEEEIGSPNLGPLLEAHRDLLACDLMVSADGGCGTPERPEVWLGPRGLVGLQINVRTADRDLHSGNGGLAPNAIHALVRLLDSMRDSAGRITVEGFYDSVRPLSDTDRAEIAAAAYPPAEYQSQTGVRGFFGEPEFTPFERASARPTLEVNGIWGGFTGEGIKTVIPCEAHAKITCRLVPDQRPAEIREKLLAHIRRHAPAYAEVTVDSLPGEADPYLIPADHPAQQALVRVLTAVTGRRPAVTRGFGTVPVMGMVKRILGVETISLGTGQADERAHAPDEFYRLDSFARGQRAYVLLLHELGEALPRTDDIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q01</accession>
    <name>PYRB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">STH1257</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195388.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q01"/>
    <dbReference type="SMR" id="Q67Q01"/>
    <dbReference type="STRING" id="292459.STH1257"/>
    <dbReference type="EnsemblBacteria" id="BAD40242">
        <property type="protein sequence ID" value="BAD40242"/>
        <property type="gene ID" value="STH1257"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1257"/>
    <dbReference type="eggNOG" id="COG0540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_2_0_9"/>
    <dbReference type="OMA" id="FPTEREY"/>
    <dbReference type="OrthoDB" id="1844275at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113214" description="Aspartate carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <sequence length="310" mass="33747" checksum="29BCE696DA600B2E" modified="2004-10-11" version="1">MARLKRKDILGLRDMTVEEIDLILDTAEALKDLATRPIKKVPTLRGKALATLFYEPSTRTAKSFELAGKYLGADVITISASTSSVQKGETLKDTARNLEAMGIDAVVLRHSMGGAPHLLARALRASVINAGDGLHEHPTQGLLDMLTIRQRKGRFKGLKVAIIGDSYHSRVARSDIFALTKYGAEVWLAGPSTLLARGFEEWGVRTTTRVEEALEGADVVIVLRLQLERQQKGLFPTVAEYHRFWGVTPERLRLARPDALLMHPGPLNRGVEISTAVADGPQSAILEQVTNGVAVRMALLYLLLGGGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q03</accession>
    <name>Q67Q03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1255"/>
    <dbReference type="EnsemblBacteria" id="BAD40240">
        <property type="protein sequence ID" value="BAD40240"/>
        <property type="gene ID" value="STH1255"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1255"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_457771_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="596" mass="63279" checksum="BEDE7C8C99178C67" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q38</accession>
    <name>Q67Q38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sporulation-specific protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spoIIGA</name>
        <name evidence="3" type="ordered locus">STH1220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195351.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1220"/>
    <dbReference type="EnsemblBacteria" id="BAD40205">
        <property type="protein sequence ID" value="BAD40205"/>
        <property type="gene ID" value="STH1220"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1220"/>
    <dbReference type="HOGENOM" id="CLU_059158_0_0_9"/>
    <dbReference type="OMA" id="ELPEAWI"/>
    <dbReference type="OrthoDB" id="1492550at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030436">
        <property type="term" value="P:asexual sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005081">
        <property type="entry name" value="SpoIIGA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03419">
        <property type="entry name" value="Peptidase_U4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02854">
        <property type="entry name" value="spore_II_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="35260" checksum="FB2B51772BDBA251" modified="2004-10-11" version="1">MSGLPAVRPDVFFLVNFGLDLALLWFAARAARVRYRAWRLWVAALLGAALAVLPVLLPDVPAAAPASPSAGPAPKAGWLFSGAALLAGSAALAWLLVWPGPWAQFAAVLGFFWTGLILSGGLLFLLAERYPALFAAPPAALVAGGAGVALAGAQLLWQAYRERAEVDDGLYELEVRVDGRREVVEGLVDSGNLLRTPVGRMPVAVVEGGRLRSLLPPAVLEAASSGPMGLDGLPAEWQSRCQLVPFAAVGRSDGWLLVIRPDGLSVRPRGRGDWVQVEGRVGLAAGPLDPEGRYAALLPTAMIAAARRAHGRARGRPVEAQSGERGEGRPDVHVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q52</accession>
    <name>Q67Q52_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase</fullName>
            <ecNumber evidence="8">6.3.2.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Meso-A2pm-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Meso-diaminopimelate-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">UDP-MurNAc-tripeptide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">UDP-N-acetylmuramyl-tripeptide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">murE</name>
        <name evidence="13" type="ordered locus">STH1206</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + meso-2,6-diaminoheptanedioate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:23676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.13" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195337.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1206"/>
    <dbReference type="EnsemblBacteria" id="BAD40191">
        <property type="protein sequence ID" value="BAD40191"/>
        <property type="gene ID" value="STH1206"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1206"/>
    <dbReference type="eggNOG" id="COG0769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022291_4_1_9"/>
    <dbReference type="OMA" id="CFMEVSS"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008765">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 8 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="12">
        <location>
            <begin position="106"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="11">
        <location>
            <begin position="331"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-MurNAc-L-Ala-D-Glu binding" evidence="8">
        <location>
            <begin position="150"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Meso-diaminopimelate binding" evidence="8">
        <location>
            <begin position="406"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Meso-diaminopimelate recognition motif" evidence="8">
        <location>
            <begin position="406"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="108"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>meso-2,6-diaminoheptanedioate</name>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="8">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="497" mass="53774" checksum="BCD56CAD8608B322" modified="2004-10-11" version="1">MRVKDCLVVGQLLQGDPAAEITDVVYDSRQVTPGALFVALPGRNVDGHDFAPEAVQRGAAALVVERPVAAPAHVAVIRTDSTRAALGRLAAEFWRHPSRRMRVVGVTGTNGKTTTTHLVKAVLERQGHVVGLTGTVHTLMPGERRPAGLTTPEASDLQRLFHQMAEAGCTYAVMEASSQGLDMNRVDDVEFDLGIFTNLTQDHLDYHGTFGAYREAKAKLFRLLSRPGYKPRKAAVLNADDPASEYYRSVTEVPVYTYGFRPDAMVRAADVELSPAGTRLRLCTPQGEVPVSLRLVGRFNVYNALAAAAAGVAEGIALDVIRDALEAEEGVPGRLQPVRAGQPFGVFVDYAHSPDSLENVLHTARGFTRGRLIVVFGCGGDRDPTKRPVMGRIAGCLAHHTIVTSDNPRSEDPVRIVQQIESGLVDGLLPGHTYEVVIDREEAIRRAVTMAEPDDVIVIAGKGHETYQIFKDRTIPFDDRAVARSLIEARVGKGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q76</accession>
    <name>Q67Q76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spore coat-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1182"/>
    <dbReference type="EnsemblBacteria" id="BAD40167">
        <property type="protein sequence ID" value="BAD40167"/>
        <property type="gene ID" value="STH1182"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1182"/>
    <dbReference type="HOGENOM" id="CLU_1395690_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023833">
        <property type="entry name" value="Signal_peptide_camelysin"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04088">
        <property type="entry name" value="cognate_SipW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="24529" checksum="DD7137CA158F57BA" modified="2004-10-11" version="1">MTERPPGSTGPGGRGLGGNPMSKKTLMVLGSAVAISALSIGGTLALFTDATDASKDFVAGTLCLRSDRNDGDPVPGPMFYITPEQGKTPSGQLGINATGPWAPGDSHTRTLTVYNSPDCSSMDAWLVSVEARLTEGIEDQYEPMAEKLHVKVKTPKRGGPDEVVAEAPLSDFLAGPVSIAYPDGTKIPLHLTSNRHMKFEVTFDKAAGNDYQDKTLVVDFIVHAEQMKNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD9</accession>
    <name>Q67QD9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase, group I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195251.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1119"/>
    <dbReference type="EnsemblBacteria" id="BAD40104">
        <property type="protein sequence ID" value="BAD40104"/>
        <property type="gene ID" value="STH1119"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1119"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_365505_0_0_9"/>
    <dbReference type="OMA" id="ISTPYWH"/>
    <dbReference type="OrthoDB" id="61917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="1">
        <location>
            <begin position="66"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="757" mass="81984" checksum="ED6362D6B463E0F5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI5</accession>
    <name>Q67QI5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">SelB translation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">selB</name>
        <name evidence="7" type="ordered locus">STH1073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195205.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1073"/>
    <dbReference type="EnsemblBacteria" id="BAD40058">
        <property type="protein sequence ID" value="BAD40058"/>
        <property type="gene ID" value="STH1073"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1073"/>
    <dbReference type="eggNOG" id="COG3276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023030_3_0_9"/>
    <dbReference type="OMA" id="YAIDRVF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015190">
        <property type="entry name" value="Elong_fac_SelB-wing-hlx_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015191">
        <property type="entry name" value="Elong_fac_SelB-wing-hlx_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004535">
        <property type="entry name" value="Transl_elong_SelB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09106">
        <property type="entry name" value="SelB-wing_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09107">
        <property type="entry name" value="SelB-wing_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00475">
        <property type="entry name" value="selB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="629" mass="67818" checksum="2F2850D16FBDC3CF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ0</accession>
    <name>Q67QJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195200.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1068"/>
    <dbReference type="EnsemblBacteria" id="BAD40053">
        <property type="protein sequence ID" value="BAD40053"/>
        <property type="gene ID" value="STH1068"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1068"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3023">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015278_2_0_9"/>
    <dbReference type="OMA" id="TYYMQLV"/>
    <dbReference type="OrthoDB" id="1584918at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14488">
        <property type="entry name" value="CBM6-CBM35-CBM36_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033803">
        <property type="entry name" value="CBM6/CBM35/CBM36-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269497" evidence="1">
        <location>
            <begin position="26"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="2">
        <location>
            <begin position="220"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="509" mass="55952" checksum="715E67C7B57ADF84" modified="2004-10-11" version="1">MRKGRGLSFFVLLLFLLTVVGTGWAAPEEFERGHRLDGPMGQAFRKAADAYGVPVELLVALAYAETRLDDHDGVPSVAGGYGVMHLVSNPQVTTLDLAAKLTGLDPARLRTDTAANILGGAALLAHYAAEQGADVKSLKLGDWFEAVARYSQLEGVAARDYALQVYEFLNRGIDAVVDGEEIRIEPQAVTPDLGRFAGLITVQSQDYGPAAWVPAHSSNYTASNRPSSYPIRYIVIHVTQGSYSGTISWFQNPSSNVSAHYVIRSSDGAITQMVREKDIAWHAGNWTYNTQSIGIEHEGWVSSCTWFTDAMYRASAALTRNIAQKYGIPMTRSYIIGHNEVPGATHTDPGSCWDWNYYMSLVTQSSGSWSAIVDNTTSGRFRASSNWGTSSWSSQRYGSNYRFAEPKAVSDAAYYKFNIPSTGNYDIYMWYPANSGYNDRTPVVIWQANSAGTGTTPVTVYVNQRQNGGRWVHLGTFRLLAGDREIIAVSRWTSGTGYVIADAFRVVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ8</accession>
    <name>Q67QJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glutamine ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195192.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1060"/>
    <dbReference type="EnsemblBacteria" id="BAD40045">
        <property type="protein sequence ID" value="BAD40045"/>
        <property type="gene ID" value="STH1060"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1060"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_1_9"/>
    <dbReference type="OMA" id="GICAELK"/>
    <dbReference type="OrthoDB" id="847343at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269498" evidence="5">
        <location>
            <begin position="28"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="6">
        <location>
            <begin position="77"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="PBPe" evidence="7">
        <location>
            <begin position="77"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32367" checksum="3A47DFE64FD116CE" modified="2004-10-11" version="1">MRKSSAFLLVILLLTALALAACGGARSSDGGQPSGGSAAQQPSGGAQAPADAGAEAPSAGESAQPASSLERIKAEGVLRVGIDASYPPMEFVDEDGKTPIGFDIDLANAVAEKLGVKADFTIIDWAGIQAGLLGGHYDVIMSSMTITEERQKEMDFVQYLTMGIAYASRPGVEVRSDADLDGKIVVVQEETTAQYYVEDLMADKGIQPKELRSFPYATDAFRELANGRADVVAIDAPVAGYYAQLDPDQYRVTGFAEMDPDPIGIALRKGETELREALQKAVEELEAEGVLRELQVKWFGDAAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R04</accession>
    <name>Q67R04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH904"/>
    <dbReference type="EnsemblBacteria" id="BAD39889">
        <property type="protein sequence ID" value="BAD39889"/>
        <property type="gene ID" value="STH904"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH904"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1022798_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027961">
        <property type="entry name" value="DUF4442"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14539">
        <property type="entry name" value="DUF4442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="28755" checksum="D1BFAF47A9F1FFFE" modified="2004-10-11" version="1">MRIPFREVIGAQVVEAGHGRSTLRMQASEHTQNNMGMVHGGALCTLADAAIGTALRSALKPGDAIATIEMKMNRSHRAAAVCWPGPAPCTSAEPRRWGGGDRGSPGQVGGEGACDLLPEARGGGAAARGTYGDDPGGGRPRTGRLDRRLLSDRGPVALRVAEERLIHIPLFVREVPERDARLTQELRQASEAQVGRMDFDQPDPRRLAQLPLQPVPLLLPLQFGGVREPLGDAAVLGSLVLQGHAVLGKEQVELHARAGLGVVQATGSPVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R34</accession>
    <name>Q67R34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sulfate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH874</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195006.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH874"/>
    <dbReference type="EnsemblBacteria" id="BAD39859">
        <property type="protein sequence ID" value="BAD39859"/>
        <property type="gene ID" value="STH874"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH874"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="AGLYPWR"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="13"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="30160" checksum="36AA5D8E9C73ED54" modified="2004-10-11" version="1">MRQPAVQPAPPELVLRSVTKHFPTRRGRIKVLDRVSLSVRKGEFLCLLGPSGCGKSTLFNIIAGLEEPDSGEVLVEGEPVAGPGPDRVLVFQDGALFPWLTALGNVEFGLSLQNIPRAERHERAMAALEMVRLARFRDAYIHELSGGMRQRVAIARALALEPKVLLMDEPFAALDAQTRNLMHEELQRIWARTGQTIIFVTHNVSEAIRLGDRIAVLSFRPGRVRREVTIQHPRPRKADDPHLLEIRTFLVRDLRSDVEAALEEELRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R49</accession>
    <name>Q67R49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH859"/>
    <dbReference type="EnsemblBacteria" id="BAD39844">
        <property type="protein sequence ID" value="BAD39844"/>
        <property type="gene ID" value="STH859"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH859"/>
    <dbReference type="HOGENOM" id="CLU_3222986_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="44" mass="4970" checksum="01113957DD059F63" modified="2004-10-11" version="1">MSVKEAHACDPGSLRSVTHCCSSRAAPFLNLRRHVPIRERVPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R66</accession>
    <name>Q67R66_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194975.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH842"/>
    <dbReference type="EnsemblBacteria" id="BAD39827">
        <property type="protein sequence ID" value="BAD39827"/>
        <property type="gene ID" value="STH842"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH842"/>
    <dbReference type="HOGENOM" id="CLU_2095633_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="12974" checksum="8D34588C4B8702FF" modified="2004-10-11" version="1">MADDLEALLLHAFIDLIEERKAAGRRELVATHETIAQWLSDRTGLNVTPRHVQYLTLALRDGQIIDIGGGGIGRPNTYDTREAQMGTDAFWDQVEAFLMVWRMPGREALRKADPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC2</accession>
    <name>Q67RC2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acid sugar phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-6 carbon acid sugars in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. NagD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194919.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH786"/>
    <dbReference type="EnsemblBacteria" id="BAD39771">
        <property type="protein sequence ID" value="BAD39771"/>
        <property type="gene ID" value="STH786"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH786"/>
    <dbReference type="eggNOG" id="COG0647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043473_1_2_9"/>
    <dbReference type="OMA" id="AFEYICR"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27993" checksum="EC2FD8E07595FE5E" modified="2004-10-11" version="1">MKRYAGYVFDLDGTLYLGDHAIPGAPETLAELRRRGARIAFLSNKPIEPAASYAAKLNRLGIQAAVEEVINSSIVMARYLSRTAPGARVYLIGEEPLAEELRKRGIRIVADPLDCEYVVVSWDRQFTYQKLNDALQAIRNGARFIATHPDRTCPVPGGEVADVGGMIGAVEGVTGKKVELITGKPSPITVQEAMNLLGLPPDQCIMVGDRLETDMRMGREAGMATALVLTGVTRREQVESSPWKPDYVLESVRGLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF0</accession>
    <name>Q67RF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH758</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194891.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH758"/>
    <dbReference type="EnsemblBacteria" id="BAD39743">
        <property type="protein sequence ID" value="BAD39743"/>
        <property type="gene ID" value="STH758"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH758"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_4_0_9"/>
    <dbReference type="OMA" id="ATSWIFT"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="61"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="27153" checksum="17933C0A8F1B95FE" modified="2004-10-11" version="1">MRKRTAGLLWGLLGIGASLVAWHLAARRYPPWAVPTPVDVARELVRLAGEGALLQAVAVTTVRTLSGFGLAVLLGGVSGLLAGLLRPLGQALAPVVTAVQGVPPVALIIVALLWFDANSTAPVFTIAVAVLPIIFAAALEGTRSVDRGLIEMATVFRTPRRMLVTEVYLPHLLSYLFPALVSGLGLAWKVALLAELMAGSRSGMGAGLGAARVNLNVAGMFAWIAVVVILLLVMENLVLRPLKRRLEPWQRGREGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH0</accession>
    <name>Q67RH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194871.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39723">
        <property type="protein sequence ID" value="BAD39723"/>
        <property type="gene ID" value="STH738"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH738"/>
    <dbReference type="HOGENOM" id="CLU_2848238_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="65" mass="7949" checksum="E77A646F4C4EBFA9" modified="2004-10-11" version="1">MRRPIRQRPIRPEYSPGTMSRELDWLVDRWQSSRPEERLQIVLAKIRAEERLEEERHEELAPVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI1</accession>
    <name>Q67RI1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH727</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH727"/>
    <dbReference type="EnsemblBacteria" id="BAD39712">
        <property type="protein sequence ID" value="BAD39712"/>
        <property type="gene ID" value="STH727"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH727"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_296806_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="467"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="4">
        <location>
            <begin position="541"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="6">
        <location>
            <begin position="623"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="5">
        <location>
            <begin position="766"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="415"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1016" mass="110035" checksum="7F633440B524D552" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK5</accession>
    <name>Q67RK5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH703</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH703"/>
    <dbReference type="EnsemblBacteria" id="BAD39688">
        <property type="protein sequence ID" value="BAD39688"/>
        <property type="gene ID" value="STH703"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH703"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="HEVLAFC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="22"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="276" mass="30923" checksum="C17E177181546A89" modified="2004-10-11" version="1">MGTTADTRCERRRWHGVSEMHLAVEEVAIGFGGVKALDGVSLAARKGSITSIIGPNGAGKTSLINCISGRYIPQQGRILFEGREITRMPPARRAELRIARTFQNIALFKHMTVLENLMVGRHALMRSGLLACGLWWGPARREEIEHRKAVEEIIDFLEIAHVRRKPVGMLPYGLQKRVELGRALALEPRLLLLDEPMAGMNAEEKEDMVRFILDINQERGVTCVLIEHDMGVVMDISDWVVVLDFGRKIGEGTPEEVRRNPEVIRAYLGDKDLAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK8</accession>
    <name>Q67RK8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative low temperature requirement C protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194833.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH700"/>
    <dbReference type="EnsemblBacteria" id="BAD39685">
        <property type="protein sequence ID" value="BAD39685"/>
        <property type="gene ID" value="STH700"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH700"/>
    <dbReference type="eggNOG" id="COG1267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110655_0_0_9"/>
    <dbReference type="OMA" id="RCNTFID"/>
    <dbReference type="OrthoDB" id="1603340at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026038">
        <property type="entry name" value="Put_PGPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019587">
        <property type="entry name" value="PGPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PgpA" evidence="1">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17556" checksum="522B17FE08A561CE" modified="2004-10-11" version="1">MYKTVVKMLEDRGVMLREIAEVVYEIQKSYYDSLPLEECLESVEAVVKKREVQNAILTGLALDMLAEEGKLPEPLQSIVAGDDFLFGVDEIVALSITNIYGSIGLTNFGYLDKTKPLVIGRLNSHKNGGCHTFLDDLVSAIAAAAASRIAHARRPSNNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS1</accession>
    <name>Q67RS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194770.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH637"/>
    <dbReference type="EnsemblBacteria" id="BAD39622">
        <property type="protein sequence ID" value="BAD39622"/>
        <property type="gene ID" value="STH637"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH637"/>
    <dbReference type="HOGENOM" id="CLU_1651284_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="18473" checksum="88AB74912A733D73" modified="2004-10-11" version="1">MFFVRRFSYLLSSLLLLVVLGLLAWRLHWQLAGYVVILGLIGVFVIYWVAVRRGARTPADPRKKLRKSRSGDRPTVLHFYSDFSLSCLLSRPLVMPVERQYRGRCEFIYVDVGHADAQAMMDELKAEMGDWLLFDRSGRLVGHRRRLTAADIERVLDGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY5</accession>
    <name>Q67RY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH573</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH573"/>
    <dbReference type="EnsemblBacteria" id="BAD39558">
        <property type="protein sequence ID" value="BAD39558"/>
        <property type="gene ID" value="STH573"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH573"/>
    <dbReference type="eggNOG" id="COG4124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027698_0_0_9"/>
    <dbReference type="OMA" id="PSAANWR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="PTHR40079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH26" evidence="5">
        <location>
            <begin position="57"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="589" mass="66569" checksum="968BF10F5505AE91" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ2</accession>
    <name>Q67RZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative sensory box/GGDEF domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH566</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH566"/>
    <dbReference type="EnsemblBacteria" id="BAD39551">
        <property type="protein sequence ID" value="BAD39551"/>
        <property type="gene ID" value="STH566"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH566"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_1_9"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="247"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="375" mass="41335" checksum="572B4DFCCF4B0261" modified="2004-10-11" version="1">MGWALVPASFVVFSPWGEGSETVARNGPLEQQKRRLLLVALPIASAAALFEWALLRRSGQAPSPLLLAGAAFALLLALLLWRRRRSLRFVETVLTAGIAVGLFVFLYLGLFSADSLSLERFARLSPWVSVALAGLLVISGTRRSVWIGTAVYLGLLALGLVRLFRAGPQPTREALFGPLAHFYLANAVLLWLMHAWSELQRQYARTRQIARSMASLAHTDPLLGIPNRRQMELLIEQEIRCAEEGGQPATMIMFDVDRFKQINDMYGHEAGDAALRAVATTVRQALRATDHVGRWGGDEFIILVRVNETAQAYQLAQRVGEAVHQKLSEHFPIVTISLGVAPYRPGDTVSSWVSRADAAMYRAKESGRNRVVVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S12</accession>
    <name>Q67S12_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194680.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH546"/>
    <dbReference type="EnsemblBacteria" id="BAD39531">
        <property type="protein sequence ID" value="BAD39531"/>
        <property type="gene ID" value="STH546"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH546"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1199303_0_0_9"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="24952" checksum="DE7BEA15D088C247" modified="2004-10-11" version="1">MRFVWLVRGELWALKRNRRILMFLAMMLGMAAIYALDGEGTADFLPVVLIVSIYSGIWQMTVNSLVEEKERRTLEALLVTPVRPLEIIGAKAFVGVALAASMSVLILLVFRRPPGAPLVLLLGIALAIGFATASGALVGIVARDLKTSSLYRTPVLAVLVGGTVIPWQDSGLAVAEVMRWLPTRPVQELMWGGWTGAPVPVAQHAAVMLAYTALLLAVDARILRRQATTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S15</accession>
    <name>Q67S15_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH543"/>
    <dbReference type="EnsemblBacteria" id="BAD39528">
        <property type="protein sequence ID" value="BAD39528"/>
        <property type="gene ID" value="STH543"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH543"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525732_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="489"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="518" mass="52661" checksum="2A168A4C282BD686" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S38</accession>
    <name>FLOA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flotillin-like protein FloA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">floA</name>
        <name type="ordered locus">STH520</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Found in functional membrane microdomains (FMM) that may be equivalent to eukaryotic membrane rafts. FMMs are highly dynamic and increase in number as cells age. Flotillins are thought to be important factors in membrane fluidity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooligomerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the flotillin-like FloA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S38"/>
    <dbReference type="SMR" id="Q67S38"/>
    <dbReference type="STRING" id="292459.STH520"/>
    <dbReference type="PRIDE" id="Q67S38"/>
    <dbReference type="EnsemblBacteria" id="BAD39505">
        <property type="protein sequence ID" value="BAD39505"/>
        <property type="gene ID" value="STH520"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH520"/>
    <dbReference type="eggNOG" id="COG4864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_836378_0_0_9"/>
    <dbReference type="OMA" id="GIMDYYR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01562">
        <property type="entry name" value="FloA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022853">
        <property type="entry name" value="FloA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12127">
        <property type="entry name" value="YdfA_immunity"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000232570" description="Flotillin-like protein FloA">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01562"/>
        </source>
    </evidence>
    <sequence length="332" mass="35440" checksum="E2F3DD256F793C01" modified="2004-10-11" version="1">MSMPGLGYLILTFVVLLLLVLFFSFVPVGLWISAAAADVRVGIFYMIGMKLRRVPPHRIVNALIKAEKAGLEISIDKLEAHYLAGGNVDRVIDALIAAQRAGIDLVFERAAAIDLAGRNVLEAVQMSVNPKVIETPVVAGVAQDGIELRAKARVTVRADINRLVGGAGEDTIIARVGEGVVSTIGSAASHKEVLENPDMISRTVLAKGLDAGTAFEIVSIDIADVDVGANIGARLRADQAEAEKVMAQAKAEERRAMAVAEEQEMRAETQRMRAKVVEAEAEVPRALAQALREGRIGVMEYLMMQNLQADTAMREALGGGQRGGQPGGQDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S41</accession>
    <name>Q67S41_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="1">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH517"/>
    <dbReference type="EnsemblBacteria" id="BAD39502">
        <property type="protein sequence ID" value="BAD39502"/>
        <property type="gene ID" value="STH517"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH517"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068847_1_0_9"/>
    <dbReference type="OMA" id="LNGADWV"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="1" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="2">
        <location>
            <begin position="168"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="34537" checksum="5475549CDC4B2242" modified="2004-10-11" version="1">MVPTEQAVGLMYAHDLTRIVPGEGKGPRFRRGQVVRPEDVPVLLAMGKEHLYVWEPHPGMLHEEEAALRLAEAGAGGGLRWDPPAEGKVSLRAAFRGLLRVDAARLEAINAIGDITIATLRDATLVEEDTVVAGCKVTPLLVAEEQIRRAEAMGRWIEVKPLAPLRAGLVVTGSEVYKGRIPDKFGPVVEEKLARFGCPVVYKAYSNDQAEMTAAKIREAVEAGAQMVLCTGGMSVDPDDATPGAIRRAGADVVTYGVPFLPGSMFLFAYLGDVPVMGLPGAVMWEKVTVFDVVLPRVLAGERLSKADFVRLGHGGLLPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S77</accession>
    <name>Q67S77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage II sporulation protein P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoIIP</name>
        <name evidence="2" type="ordered locus">STH481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH481"/>
    <dbReference type="EnsemblBacteria" id="BAD39466">
        <property type="protein sequence ID" value="BAD39466"/>
        <property type="gene ID" value="STH481"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH481"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040895_1_0_9"/>
    <dbReference type="OMA" id="QQSFPFE"/>
    <dbReference type="OrthoDB" id="1416338at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010897">
        <property type="entry name" value="Spore_II_P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07454">
        <property type="entry name" value="SpoIIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02867">
        <property type="entry name" value="spore_II_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="40573" checksum="51E7800187F54EC5" modified="2004-10-11" version="1">MVVAATVGLLVNRRPPESGSLPAMTARASDGRTHESERTEESFWTALFRPGLPTARQMLRRAVPALAVRGPLGEPDDRVLRFLWTGPGPQRPQTLFQAALPFLRPGALPEEPMTVDPSLPAGESPGPEPRPSPPAAAEAPFPRPEPGATVLNDGLPLVGIYHTHDYEAYISEFPDLAVTSDQDLQRIASYDHSKRTIVDIGAILARRLRDLGVTTVHAPFKHQELGYEYAYQSSRNTARRILREAPTVKVLMDLHRDGNMDLDSTVWIDGQPVARVRCVIGVRDDLTHWQENLAFCNRLMEKMEEANPGITLPTLTPQARYNQDLLPGAILLEIGNALNTFEEAERAVYYLADALVELLRAGEYPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB5</accession>
    <name>Q67SB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH443"/>
    <dbReference type="EnsemblBacteria" id="BAD39428">
        <property type="protein sequence ID" value="BAD39428"/>
        <property type="gene ID" value="STH443"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH443"/>
    <dbReference type="HOGENOM" id="CLU_210189_5_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="40" mass="4235" checksum="2602DE1F083EF650" modified="2004-10-11" version="1">MPQTTGLLPGSIAMIIFGAALLWGGLAFFITTALRAEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC3</accession>
    <name>Q67SC3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase (symmetrical)</fullName>
            <ecNumber evidence="2">3.6.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH435</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.41"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.1.41" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194569.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH435"/>
    <dbReference type="EnsemblBacteria" id="BAD39420">
        <property type="protein sequence ID" value="BAD39420"/>
        <property type="gene ID" value="STH435"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH435"/>
    <dbReference type="eggNOG" id="COG1713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089580_1_2_9"/>
    <dbReference type="OMA" id="ILTRNWA"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005249">
        <property type="entry name" value="YqeK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00488">
        <property type="entry name" value="TIGR00488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="4">
        <location>
            <begin position="21"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="20768" checksum="AA877EC0C17E001B" modified="2004-10-11" version="1">MATADWRTLEGVLQRQVTGERLAHTYRVLEYARRLGRIHGADPDQVAVAALMHDYAKPLPAETLLREAERLGLSVHPVERAAPHLLHGPVAAAQLAEQGLVTDREVLQAIATHTTGRAGMGLLEKVLYVADYAEPGRRFPGAAEVREAAEQDLDRALLAALNNSLLYLVGQGWLIHPASVEARNWLLQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE3</accession>
    <name>Q67SE3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194549.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH415"/>
    <dbReference type="EnsemblBacteria" id="BAD39400">
        <property type="protein sequence ID" value="BAD39400"/>
        <property type="gene ID" value="STH415"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH415"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_9"/>
    <dbReference type="OMA" id="LEVYIAM"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="228" mass="25525" checksum="D5B181520A605A6F" modified="2004-10-11" version="1">MSLRVLVVDDDPKITAFLRRSLTLEGYDVAVANSGTEALRSVTASPPDLMVLDIMMPDVDGLEVCRRIRAAGETFPILMLTARDAVSDRVKGLDQGADDYLVKPFALEELLARLRALSRRGAPAQGEDGHRVLQYADLRLDTATREAYRGDRQISLTAKEYELLHLFLSHPRQVLTRDTLMEKVWGYDYSGESNVLEVYVAYLRNKLEAGGEPRLIQTVRGVGYVLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE8</accession>
    <name>Q67SE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH410"/>
    <dbReference type="EnsemblBacteria" id="BAD39395">
        <property type="protein sequence ID" value="BAD39395"/>
        <property type="gene ID" value="STH410"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH410"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_3_9"/>
    <dbReference type="OMA" id="PVPLYQA"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="11247" checksum="0BC610933504AE5B" modified="2004-10-11" version="1">MQARLCQVLASPKRLEILYTLKDQEMTAGELAKAVDVTMPNLSQHLSLMKQHGLVESRKEGLNMYYRVASHQILETLASVRKVLVEQLAKQSKLLEQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV0</accession>
    <name>Q67SV0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methionine synthase I, cobalamin-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH258"/>
    <dbReference type="EnsemblBacteria" id="BAD39243">
        <property type="protein sequence ID" value="BAD39243"/>
        <property type="gene ID" value="STH258"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH258"/>
    <dbReference type="eggNOG" id="COG5012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_495139_0_0_9"/>
    <dbReference type="OMA" id="MAIKPHI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="543" mass="59095" checksum="604FA48C49BF64CB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV1</accession>
    <name>Q67SV1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD/NADP octopine/nopaline dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH257</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH257"/>
    <dbReference type="EnsemblBacteria" id="BAD39242">
        <property type="protein sequence ID" value="BAD39242"/>
        <property type="gene ID" value="STH257"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH257"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056511_2_0_9"/>
    <dbReference type="OMA" id="LMMLMNA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003421">
        <property type="entry name" value="Opine_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02317">
        <property type="entry name" value="Octopine_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_Gly3P_dh_N" evidence="2">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Octopine_DH" evidence="3">
        <location>
            <begin position="184"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="364" mass="38631" checksum="71A80A1EB4B546F8" modified="2004-10-11" version="1">MLRYCVIGAGHGGLAMAGHLGVMGFPVSLWARNPQTLEPIEAVGGVFLEGEVEGFGPVQVAYSLEAALEGADAVLVVLPASAHREIAMRCAPYLRDGQRILLLPGRTAGAIEFRHSLREAGCTADVIVGEAQTFLYASRKTGPFQARIHGIKRQVPAAALPASRTGELLEAIKPAFPQFMPARWVWKTSLDNVGAIFHPGVALLNAGRIESTGGAFRHYVDGITPSVAALLEQLDRERVAVARALGVGALTAREWLAEAYGVERPTLYEAIQATRAYADVGAPAQLHHRYIWEDVPTGLVPIASLGAACGVSTPVADALIQIANGIFGVDFRAMGRTVERLGMAGWGPARITQYALEGDVVDVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW6</accession>
    <name>PGK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk</name>
        <name type="ordered locus">STH242</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194377.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SW6"/>
    <dbReference type="SMR" id="Q67SW6"/>
    <dbReference type="STRING" id="292459.STH242"/>
    <dbReference type="EnsemblBacteria" id="BAD39227">
        <property type="protein sequence ID" value="BAD39227"/>
        <property type="gene ID" value="STH242"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH242"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_9"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="OrthoDB" id="462069at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000058074" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="347"/>
            <end position="350"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <sequence length="392" mass="41999" checksum="3BC18CE27BB08EAE" modified="2004-10-11" version="1">MALMTVRDIDVKGKRVFVRVDFNVPLEDGRITDDTRIRAAVPTIRYLVEEGAVAVLASHLGRPKGQRNEKYSLAPCARRLSELLGREVVFAPDCIGEEVERLVAEQPPGSVVLLENVRFYAEEEKNDPEFAAKLARLGEIFVNDAFGTAHRAHASTRGVADHMPVRVAGFLMQKEVDIMGKALSDPDRPFVAIIGGAKVSDKIMVIENLLTKVDRLIIGGGMANTFLRARGFATGKSLVEEDRVDTARELLAKGGDKILLPTDLVVASEFKADAEQKVVPVDAIPEGWMALDIGPETARAFADVIRGAHTVVWNGPMGVFEMEPFARGTFAVAQAMADCEGVTIVGGGDSVAAVEAAGLADRMTHVSTGGGASLEFLEGKELPGVACLAVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX0</accession>
    <name>Q67SX0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH238"/>
    <dbReference type="EnsemblBacteria" id="BAD39223">
        <property type="protein sequence ID" value="BAD39223"/>
        <property type="gene ID" value="STH238"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH238"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="13"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="29892" checksum="E8B8E14914B18B9E" modified="2004-10-11" version="1">MNQAMRNASGAKLVTRGLRVYYGGREALRGVSLAVPDRSVTALIGPSGCGKSTFLRALNRMHDLRHGVRVEGRILLDGLPVERLPPEELRRRIGMVFQRPNPFPMSIFQNVAFGPALLGVRGPELERIVRSALQRAALWSEVKDRLHQHAGGLSGGQQQRLCLARALAVEPEVLLLDEPCSALDPAATDEVERLIAELRGAMAIVLVTHSLDQARRLADRVAFFQDGRLVEEGPAEEVLQRPRQPETRRFLERSDWHGICRGNRGRQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ6</accession>
    <name>Q67SZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH212"/>
    <dbReference type="EnsemblBacteria" id="BAD39197">
        <property type="protein sequence ID" value="BAD39197"/>
        <property type="gene ID" value="STH212"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH212"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_9"/>
    <dbReference type="OMA" id="RCIKEAV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27942" checksum="E68DFD89B11DA10C" modified="2004-10-11" version="1">MAYENILVFREGAVGRVQLNRPQALNALNFRLMTELVEAFREMERDPGIGCLLLHGNEKAFAAGADIKEMAELGAIDMYTRDYIELWDEVARIKKPIVAAVSGYCLGGGLELAMICDIIVASETAKFGQPEINIGVLPGAGGTQRLTRAVGKSLAMEMVLTGRMLTAQEALAAGLVSRVVPVEVYLSEAQKLAGEIASKSPVALRLAKEAVLQAFEGSLSSGLAYERRLFAMAFASEDQKEGMRAFTEKRKPEWKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T33</accession>
    <name>Q67T33_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH175"/>
    <dbReference type="EnsemblBacteria" id="BAD39160">
        <property type="protein sequence ID" value="BAD39160"/>
        <property type="gene ID" value="STH175"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH175"/>
    <dbReference type="HOGENOM" id="CLU_1325803_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="22318" checksum="90DB2F7037CB6222" modified="2004-10-11" version="1">MAAVQEDRLMVLQMLADGRLTVEEADTLLRSLEETAAHADAAGAGPARRADSAADRHEPGARLRVLLQQMLDEANAALDRALRSVEERLAELERREPYRQIRQLADSLDRVAAREAVREARAAAKEAVREARAAAKEAVEEALEEVEEKVADAIETAKEAVADAIEAAEEAVADALEAAEEAVADAAHAAEEAEENVRFRASGRFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T71</accession>
    <name>Q67T71_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH137"/>
    <dbReference type="EnsemblBacteria" id="BAD39122">
        <property type="protein sequence ID" value="BAD39122"/>
        <property type="gene ID" value="STH137"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH137"/>
    <dbReference type="eggNOG" id="ENOG5032Y5Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117097_1_0_9"/>
    <dbReference type="OMA" id="MVRGGYT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269371" evidence="1">
        <location>
            <begin position="35"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="143" mass="14915" checksum="4F682983DA55BE98" modified="2004-10-11" version="1">MEGSELRMPHRWRNLSLLVAAALLVGLAAGRVMAETAEPGTGGDPLVSKSYVDLVALYRVVEVSAGQKVIGEAGTELVLRGGRATAIDSALGGLLDVTAGTDIRGGSRIPANHLIVVPRSDGRGFQAETDLVLMVKGRVTIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T76</accession>
    <name>Q67T76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">L-cysteine/cystine lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194267.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH132"/>
    <dbReference type="EnsemblBacteria" id="BAD39117">
        <property type="protein sequence ID" value="BAD39117"/>
        <property type="gene ID" value="STH132"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH132"/>
    <dbReference type="eggNOG" id="COG0520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_1_9"/>
    <dbReference type="OMA" id="SDCEHPG"/>
    <dbReference type="OrthoDB" id="446447at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="4">
        <location>
            <begin position="19"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="41312" checksum="8B8F13B1C364A9F2" modified="2004-10-11" version="1">MEKLTQIRSQLPALSSQVYLNTGTAGPLPEPVRAAMVESLAAQAAEGRIATEYYVKLNQLKAQVRAKHAALLGCEPEEIALTQNTTDGMNLVTLGVNWQPGDEAITTNLEHPGALFPLFAARERYNITIRIADVLNRPEEAPAVIERLITPRTKLISLSHVSFITGAVLPIREIAEVAHRHGVLVLVDGAQSFGAIPVNVKELGVDFYAVTGQKWLCGPEGVGALYGSRTAAAQLRITFAGYGTAQAYDMYAGLLPKGGVQKLEQGTVQPANLAGQLAACRWLTEEVGTDWAYARIRRLAATARELLARVPGVTVVTPAETAGLVTFQVETDAERVLKALAQQRIIVRTVPHPRSLRLSAGFYNTEEELEQLAEALRPLAQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA3</accession>
    <name>Q67TA3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194240.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH105"/>
    <dbReference type="EnsemblBacteria" id="BAD39090">
        <property type="protein sequence ID" value="BAD39090"/>
        <property type="gene ID" value="STH105"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH105"/>
    <dbReference type="eggNOG" id="COG0425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_0_1_9"/>
    <dbReference type="OMA" id="DCLGDMC"/>
    <dbReference type="OrthoDB" id="2042068at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8647" checksum="F174389262250970" modified="2004-10-11" version="1">MATYTLNVYGEMCPAPLLKAEAKLRSMQPGDLLIMESDHSCTVRLLREHLRKLPCRFRVEEVADGIWQFRIERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB2</accession>
    <name>Q67TB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-lactate permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH96</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transports L-lactate across the membrane. Can also transport D-lactate and glycolate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the lactate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194231.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH96"/>
    <dbReference type="EnsemblBacteria" id="BAD39081">
        <property type="protein sequence ID" value="BAD39081"/>
        <property type="gene ID" value="STH96"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH96"/>
    <dbReference type="eggNOG" id="COG1620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021628_0_0_9"/>
    <dbReference type="OMA" id="TNEMAYA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015129">
        <property type="term" value="F:lactate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003804">
        <property type="entry name" value="Lactate_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30003">
        <property type="entry name" value="PTHR30003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02652">
        <property type="entry name" value="Lactate_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00795">
        <property type="entry name" value="lctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="578" mass="60573" checksum="A108C23D1EB6C4D8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD6</accession>
    <name>Q67TD6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH72</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194207.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH72"/>
    <dbReference type="EnsemblBacteria" id="BAD39057">
        <property type="protein sequence ID" value="BAD39057"/>
        <property type="gene ID" value="STH72"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH72"/>
    <dbReference type="eggNOG" id="COG3872">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038140_0_0_9"/>
    <dbReference type="OMA" id="LFQYHGA"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010787">
        <property type="entry name" value="DUF1385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07136">
        <property type="entry name" value="DUF1385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="32749" checksum="E62CD0EAB5B74D27" modified="2004-10-11" version="1">MSERGGAGVSVQRGVFGGQAVIEGVMMRGPQWMAIAVRRPDGEIAVHREEVRSLMQRYPVLRLPILRGMVAMVEALTLGISALMFSANQSMPEDEQLSRGEMTVTTVVGFAFAIALFVVAPTLLTNYVKALSVTPMVTHVVEGALRLGLFIGYLVAISLMKDIQRVFQYHGAEHKVINAYEAGDELTVENVRRHSREHKRCGTSFLLYVVVISILIFSLINVESVWLRVVARIGMLPLVAGVAYEWIRIAGRHDNALVNALSRPGMWLQGLTTREPDDSQIEVAIASFETARGHGLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG7</accession>
    <name>Q67TG7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH41</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194176.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH41"/>
    <dbReference type="EnsemblBacteria" id="BAD39026">
        <property type="protein sequence ID" value="BAD39026"/>
        <property type="gene ID" value="STH41"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH41"/>
    <dbReference type="eggNOG" id="COG1078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_0_0_9"/>
    <dbReference type="OMA" id="FSHAMEH"/>
    <dbReference type="OrthoDB" id="370035at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="49"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="47765" checksum="0D3645C72F775D7C" modified="2004-10-11" version="1">MKEYIFRDPIHGNIAVKDETILRLIQSPEFQRLRRIRQLGTSFISYPGAEHTRFAHSLGVYHLMGRVLRHLVEHRVEIGEEEQAMARAAALLHDIGHGPFSHLFEKVTGMNHEAWVARIITSPESTVAHILAERDPAWPARVASFIRGVWEGRPFLKELIASQLDVDRMDYLLRDSRMCGVTYGQFDLERLIQTVTVVDDHIALTDKGITSAEEFLLARYFMYWNVYFHKATRSSEVLLELALRRAVALVRGGEQAALGFLPPALEPVLAGEELSLDQYVALDETDVLYAIKRWTAAPDPVLADLSGRFLHRRLFAGIRLDGGLDPEQEEGVRRALRAAGFDPDYYYQIDRAASATYQYYVAQDAGPTPIKILLSWTDPPELREMTEVSQVLRGIATQPVSRSFLFVPREAAERVRAAVMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI2</accession>
    <name>Q67TI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Two-component response regulator variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH26</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH26"/>
    <dbReference type="EnsemblBacteria" id="BAD39011">
        <property type="protein sequence ID" value="BAD39011"/>
        <property type="gene ID" value="STH26"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH26"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_103_8_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8926" checksum="B471256CCFAEE6CF" modified="2004-10-11" version="1">MATSRYEELTPREQEVFRLLVDGYTSKAIARALYISPKTADKHRASIMRKLEVNTFPELVRYAVSIGLLEVEPQESVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1993-07-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>P31014</accession>
    <accession>Q67SB7</accession>
    <name>TNAA1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Tryptophanase 1</fullName>
            <ecNumber>4.1.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>L-tryptophan indole-lyase 1</fullName>
            <shortName>TNase 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tnaA1</name>
        <name type="synonym">tna-1</name>
        <name type="ordered locus">STH441</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1992" name="Appl. Environ. Microbiol." volume="58" first="2633" last="2642">
            <title>Cloning, nucleotide sequences, and overexpression in Escherichia coli of tandem copies of a tryptophanase gene in an obligately symbiotic thermophile, Symbiobacterium thermophilum.</title>
            <authorList>
                <person name="Hirahara T."/>
                <person name="Suzuki S."/>
                <person name="Horinouchi S."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="1339259"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-36</scope>
        <source>
            <strain>IAM 13621</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + L-tryptophan = indole + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19553"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16881"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="EC" id="4.1.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid degradation; L-tryptophan degradation via pyruvate pathway; indole and pyruvate from L-tryptophan: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-eliminating lyase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD39426" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.1.99.1"/>
    <dbReference type="EMBL" id="AB010832">
        <property type="protein sequence ID" value="BAA24688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39426.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A49022">
        <property type="entry name" value="A49022"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P31014"/>
    <dbReference type="SMR" id="P31014"/>
    <dbReference type="STRING" id="292459.STH441"/>
    <dbReference type="EnsemblBacteria" id="BAD39426">
        <property type="protein sequence ID" value="BAD39426"/>
        <property type="gene ID" value="STH441"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH441"/>
    <dbReference type="eggNOG" id="COG3033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047223_0_0_9"/>
    <dbReference type="OMA" id="EMYQYGD"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-7584"/>
    <dbReference type="UniPathway" id="UPA00332">
        <property type="reaction ID" value="UER00452"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009034">
        <property type="term" value="F:tryptophanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00544">
        <property type="entry name" value="Tryptophanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011166">
        <property type="entry name" value="Beta-eliminating_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013440">
        <property type="entry name" value="TNase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018176">
        <property type="entry name" value="Tryptophanase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001386">
        <property type="entry name" value="Trpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00853">
        <property type="entry name" value="BETA_ELIM_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="chain" id="PRO_0000195622" description="Tryptophanase 1">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAA24688." evidence="2" ref="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="454" mass="51083" checksum="C13518B3F5CD6555" modified="2004-11-23" version="2">MPKGEPFKIKMVEPIRLIPREDRERALKEAHYNPFFLRSSDVYIDLLTDSGTGAMSQFQWSAMMLGDESYAGASSYYRLKETVTDITGYEYVIPTHQGRGAEKVAFSQLITRPGMYVLSNMFFDTTRGHVQLAGGRPVDLLIDVPTEEYHPFKGNMDTERLEQFIREHGAENIACIVMTVTNNSAGGQPVSMANIRETYQIARKYGVLVLFDVARYAENCHFIRMREEGYADKHPIDIAREMFSYGDGLMMSAKKDALVNIGGLLAFRDEELFTKVGAAVVPFEGFLTYGGLAGRDLEAMAVGLREALDPDYLAYRVGQVQYLGEMLRNAGIPIQWPVGGHAVFIDAAKFLPHVPWDQFPGHALTLALYLEGGVRTVEVGSLMIGRDPETGENVRGPFEFTRLAIPRRVYTNLHLEDVAETVINAFQKREEIRGVKFAREPKVLRHFTAWFDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB8</accession>
    <name>Q67JB8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197363.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3250"/>
    <dbReference type="EnsemblBacteria" id="BAD42232">
        <property type="protein sequence ID" value="BAD42232"/>
        <property type="gene ID" value="STH3250"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3250"/>
    <dbReference type="eggNOG" id="COG3583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036884_0_0_9"/>
    <dbReference type="OMA" id="EAEASNW"/>
    <dbReference type="OrthoDB" id="2027419at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010611">
        <property type="entry name" value="3D_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007137">
        <property type="entry name" value="DUF348"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011098">
        <property type="entry name" value="G5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06725">
        <property type="entry name" value="3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03990">
        <property type="entry name" value="DUF348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07501">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01208">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="SSF50685"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51109">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269226" description="G5 domain-containing protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G5" evidence="3">
        <location>
            <begin position="107"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="30774" checksum="B65980BE09E70682" modified="2004-10-11" version="1">MHLKSLRGRALAIAAAAVLLAGALGQLGRPAQHTVTTATESAPAGEVLAAAATVPVMLIDGGERRTVQTAAHTVGELLKEQRVTLGELDSLSVPESAAVTAGLEVRIVRRTEELVTEEEEIPFDTVDRPDGSLVVGAEEVVQPGAPGLKLVERLVRYEDGVVVSSEVVTERVVQEPTPQIVAYGTGGVVSRGGERFRYTEVLEMVATGYTPGPESNPGGVGLTYTGIPAARGIVAVDPQVIPLYTRLYIEGYGPALAADTGGAIKGNRIDLCFDTVEEALAWGVRPVTVYVLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG2</accession>
    <name>Q67JG2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197319.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3206"/>
    <dbReference type="EnsemblBacteria" id="BAD42188">
        <property type="protein sequence ID" value="BAD42188"/>
        <property type="gene ID" value="STH3206"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3206"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1824345_0_0_9"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="89"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="141" mass="15720" checksum="B7815A028406E0E5" modified="2004-10-11" version="1">MTLVRDAMIQVEPLSPNDPIFVALQAMRKQRLPFVPVVRSDGTLYGLVTEGDLVRLIYRATRESGESVPHWIRGAGRQVLLVQSVKEVVTRELDTIGPDIPLEDAAELMVRNHRKVLPVVADGRPMGYLMRSAVVDHLIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH6</accession>
    <name>Q67JH6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamate formimidoyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3192</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.5" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3192"/>
    <dbReference type="EnsemblBacteria" id="BAD42174">
        <property type="protein sequence ID" value="BAD42174"/>
        <property type="gene ID" value="STH3192"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3192"/>
    <dbReference type="eggNOG" id="COG3643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040037_0_0_9"/>
    <dbReference type="OMA" id="DEDHNRL"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00555"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030409">
        <property type="term" value="F:glutamate formimidoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013802">
        <property type="entry name" value="Formiminotransferase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037070">
        <property type="entry name" value="Formiminotransferase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004227">
        <property type="entry name" value="Formiminotransferase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012886">
        <property type="entry name" value="Formiminotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037064">
        <property type="entry name" value="Formiminotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022384">
        <property type="entry name" value="FormiminoTrfase_cat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02971">
        <property type="entry name" value="FTCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07837">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01221">
        <property type="entry name" value="FTCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01222">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55116">
        <property type="entry name" value="SSF55116"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02024">
        <property type="entry name" value="FtcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0290">Folate-binding</keyword>
    <keyword evidence="5" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FTCD_N" evidence="8">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="FTCD" evidence="7">
        <location>
            <begin position="173"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="290" mass="31160" checksum="6F9F7ABEB7CB75CD" modified="2004-10-11" version="1">MPNFSEGRRPEVIEAIAGAIAAQGVKVLSVSADVDHNRCVVTFIGSPEAVVRAALRGAEAAIRLIDLNHHKGSHPRMGAVDVIPFVPVSGCTMEDCVALARQVGEALGNMGVPVFLYEEAATRPDRRNLADVRRGEFEGLRELIGVDPAKDPDFGPRKIHPTAGCTAVGARMPLIAFNVNLGTSDLSIARKIAKAVRGSSGGLVHCKAIAVPLADRNQVQVSMNMVNYKETPLHRAFELIRIEAERYGVPVVGSEIVGLVPVDALVGVARHYLRLEGFKTDQVLEKRLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL7</accession>
    <name>Q67JL7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197264.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3151"/>
    <dbReference type="EnsemblBacteria" id="BAD42133">
        <property type="protein sequence ID" value="BAD42133"/>
        <property type="gene ID" value="STH3151"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3151"/>
    <dbReference type="HOGENOM" id="CLU_2921097_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="61" mass="6968" checksum="F40D67E212FC7D05" modified="2004-10-11" version="1">MDIEWTRFSDLSVSSGFLLIAVLVLWAAVAVVMYWAFMSGSTSMNEDVKFKYVEDDKPITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR0</accession>
    <name>Q67JR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197223.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3108"/>
    <dbReference type="EnsemblBacteria" id="BAD42090">
        <property type="protein sequence ID" value="BAD42090"/>
        <property type="gene ID" value="STH3108"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3108"/>
    <dbReference type="eggNOG" id="COG2770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_4_9"/>
    <dbReference type="OMA" id="HTWEINT"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAMP" evidence="4">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="126"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="187"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="271"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="425" mass="46652" checksum="72F165DCDE81281A" modified="2004-10-11" version="1">MTDKTSLDHAVEALRGLLADPKADAEPPPELQHHAGFAALYRDLSALRPHLTRICQGDLSHPIHEQGPLAGCRKSLQARLRHLTWQVQMVAAGDFSQRGESLGEFSTAFNEMTEALARARAELQRSEARYRLLAEHVADVIVTLDGEGRFLYVSPSAQALLGYAPDELAGRPLCDITPSAEAPRPGTVVELTVRCRDGTTRWAEVNTAALPPGAGDGAVLVCVFRDISARKRLESDLKHLATTDGLTGAYNRRSFVEVCGQELQRADRRARPTSLLLMDIDHFKRLNDRFGHAAGDEVLRQIVETGRRVLRSEDVVGRIGGDEFAVLLPATTLEQAGRVAEQLRTTYERLAVPARGGTATFTVSFGVAEWKPHLRTVEELLQRADRALYAAKNAGRNCVRTEEACGGRPQQDPPPQGRNCIVLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR6</accession>
    <name>Q67JR6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative formate dehydrogenase formation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197217.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3102"/>
    <dbReference type="EnsemblBacteria" id="BAD42084">
        <property type="protein sequence ID" value="BAD42084"/>
        <property type="gene ID" value="STH3102"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3102"/>
    <dbReference type="eggNOG" id="COG3058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071015_1_1_9"/>
    <dbReference type="OMA" id="DLFCEDL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16341">
        <property type="entry name" value="FdhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024064">
        <property type="entry name" value="FdhE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006452">
        <property type="entry name" value="Formate_DH_accessory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37689">
        <property type="entry name" value="PTHR37689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04216">
        <property type="entry name" value="FdhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144020">
        <property type="entry name" value="SSF144020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="29193" checksum="2368143D23C835E6" modified="2004-10-11" version="1">MQSLEFLRAWRERARALEAELPGPERLPAPEEAEAALGRGEPLITLVEPPIDPDRFTAVLADLASLYAESRADARPLAEGLRALPPAEQRTLAEVLVRAGDAAEWAARLGVDEGLLLTLGGLALQPFMARFARAVRAVAPLHGWRRIHCPVCGASPDLCRIDPDNYRYLHCPQCDTQWEHHRLTCAVCGSDDVRRVSILTLADLEPWRVEVCDRCGGYIKTLDQRHGGHLAMPNVDLYLEDARTLQLDLLAEQEGYRRGGRAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT9</accession>
    <name>RS7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsG</name>
        <name type="ordered locus">STH3079</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197194.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JT9"/>
    <dbReference type="SMR" id="Q67JT9"/>
    <dbReference type="STRING" id="292459.STH3079"/>
    <dbReference type="EnsemblBacteria" id="BAD42061">
        <property type="protein sequence ID" value="BAD42061"/>
        <property type="gene ID" value="STH3079"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3079"/>
    <dbReference type="eggNOG" id="COG0049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_1_1_9"/>
    <dbReference type="OMA" id="NVMPHVE"/>
    <dbReference type="OrthoDB" id="1540940at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000124362" description="30S ribosomal protein S7">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <sequence length="156" mass="17850" checksum="008F46BBCE552152" modified="2004-10-11" version="1">MPRRGHVPKREVQPDPVYNSELVTRVINKIMLDGKKALAQRIFYEAIDKASAKLGKEPMEVLEGALKNIMPVVETRSRRVGGATYQVPMEVRPERRQALGIRWLVQFARARGERTMVERLAAELVDAFNNTGGAVKKKEEVHRMAEANKPFAHYRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW9</accession>
    <name>RS11_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsK</name>
        <name type="ordered locus">STH3049</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197164.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW9"/>
    <dbReference type="SMR" id="Q67JW9"/>
    <dbReference type="STRING" id="292459.STH3049"/>
    <dbReference type="EnsemblBacteria" id="BAD42031">
        <property type="protein sequence ID" value="BAD42031"/>
        <property type="gene ID" value="STH3049"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3049"/>
    <dbReference type="eggNOG" id="COG0100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_5_0_9"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="1713391at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000123239" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <sequence length="129" mass="13949" checksum="C4248019DE2F8BF7" modified="2004-10-11" version="1">MAKRTARVKRRERKNVDKGVAHIRSTFNNTIVTITDVHGNALAWATAGAMGFKGSRKSTPFAAQMAAEKAAKDAMEHGVREVEVLVKGPGSGREAAIRQLQAAGLEVTAIKDVTPIPHNGCRPPKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX5</accession>
    <name>Q67JX5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197158.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3043"/>
    <dbReference type="EnsemblBacteria" id="BAD42025">
        <property type="protein sequence ID" value="BAD42025"/>
        <property type="gene ID" value="STH3043"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3043"/>
    <dbReference type="eggNOG" id="COG0619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_2_2_9"/>
    <dbReference type="OMA" id="MKAQMSR"/>
    <dbReference type="OrthoDB" id="1479665at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="268" mass="29201" checksum="46968458857FA1CD" modified="2004-10-11" version="1">MSFQVAIGQYYPGNSLLHRLDPRVKITGVFLLSIAIFLPRAWPGHLAAVLFTLALVALGRVPLKQILRGLRPILIFLVITALFNLFLTPGEELFRIGPAVATREGLRLVAVTSTRLLLLVTIASILTLTTSPIRLTDGLEMMLRPLRPIGVPAHELALMMTIALRFIPTLAEEADRIMRAQQARGASFATGSLVSRVRSLVPVLVPLFVAAFRRADELATAMEARGYRGGEGRTRMKELTFTARDAVALVVLIGFVALMVVMRIGGVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY2</accession>
    <name>LEU3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="1">1.1.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-IPM-DH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Beta-IPM dehydrogenase</fullName>
            <shortName evidence="1">IMDH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuB</name>
        <name type="ordered locus">STH3035</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197150.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JY2"/>
    <dbReference type="SMR" id="Q67JY2"/>
    <dbReference type="STRING" id="292459.STH3035"/>
    <dbReference type="EnsemblBacteria" id="BAD42018">
        <property type="protein sequence ID" value="BAD42018"/>
        <property type="gene ID" value="STH3035"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3035"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_3_9"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="OrthoDB" id="1551125at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01033">
        <property type="entry name" value="LeuB_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="PTHR42979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000083765" description="3-isopropylmalate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01033"/>
        </source>
    </evidence>
    <sequence length="368" mass="39411" checksum="675D601995BFD9F9" modified="2004-10-11" version="1">MAQPTYHIACLPGDGIGPEVTRGAVTVLQAAAAAYGFRLEFSEYLVGGAAYDAVGTPFPDETRDACDRADAILFGAVGGPRYEGLPWDLRPEAGLLAIRKRYDLYANLRPILLYPPLKDASPLKNEIIGGGVDFIIVRELVGGIYFGEPRGIETLPDGTRRGVNTEVYTDAEIARIARMGFEIARGRRRRLTSVDKGNVMEAGKLWRTVVDEVAREFPDVEVEHLLADNAAMQILRRPGDFDVMLASNLFGDFLSDEAAMLTGSIGLLPSASLGAARNRFGLPKGFYEPIHGSAPDIAGQDRANPLAAILCGAMLLRHSLGREDAARAVEQAVAAVLEEGLRTADITVPGTAVLGTAAMARAVADRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K18</accession>
    <name>Q67K18_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar FliJ protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fliJ</name>
        <name evidence="11" type="ordered locus">STH2999</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliJ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197114.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2999"/>
    <dbReference type="EnsemblBacteria" id="BAD41982">
        <property type="protein sequence ID" value="BAD41982"/>
        <property type="gene ID" value="STH2999"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2999"/>
    <dbReference type="HOGENOM" id="CLU_1739602_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012823">
        <property type="entry name" value="Flagell_FliJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02050">
        <property type="entry name" value="FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02473">
        <property type="entry name" value="flagell_FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="150" mass="17659" checksum="8FC44E008B8BBEA9" modified="2004-10-11" version="1">MKRFRFRLQRLLEIRQQETKVALNNFARARLATRAAAMRLEQAAARRAESAQRLLARRQGRMTVLEWRQSTELHEALVAAEWLAAEQLAAAQQEEERRRWELTEAERREKVLERLRDRRAAEFERAMLAAEQAAIDEMAQTVYREGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K42</accession>
    <name>Q67K42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Flagellar motor protein MotA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">motA</name>
        <name evidence="9" type="ordered locus">STH2973</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197088.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2973"/>
    <dbReference type="EnsemblBacteria" id="BAD41956">
        <property type="protein sequence ID" value="BAD41956"/>
        <property type="gene ID" value="STH2973"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2973"/>
    <dbReference type="eggNOG" id="COG1291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079895_0_0_9"/>
    <dbReference type="OMA" id="KLQGFME"/>
    <dbReference type="OrthoDB" id="897037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="101"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="27873" checksum="D5778F15560A4152" modified="2004-10-11" version="1">MDLATIGGLLFAIIAIAVGAYLEHVTLDSLLGISAFLIVVGGSIGATIVSSAVEDLKQVPRVLKMAFSPPKRDYQGMIDLLVGLAEKARRNGLLSLQEDAEHAENPVVQRGLTMAVDGADPEQVVAVMRAMSEQQADEIIRGAAVFDTAGGYAPTLGIMGTVMGLITIMGRLSEPDTLGPAIAVAFLATLYGLGSANLFFLPMGYKIKAIAKQLTHFNEMIIVGIEGIQAGENPRNLREKLSIYLPSTGGRRAAVTEGAPVREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K51</accession>
    <name>Q67K51_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation efflux system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2964</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2964"/>
    <dbReference type="EnsemblBacteria" id="BAD41947">
        <property type="protein sequence ID" value="BAD41947"/>
        <property type="gene ID" value="STH2964"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2964"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_5_9"/>
    <dbReference type="OMA" id="IEMHLIV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="7">
        <location>
            <begin position="247"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="327" mass="34193" checksum="3E1CD40918B6097B" modified="2004-10-11" version="1">MRVLCRLGKVSSGQLSFTAETPADREKAGCAPIDTTSARQAQSQKGAWLSVATYCLLSAAKLTVGWLSGSQAIAADGINNATDVLGSAAVLLGVKIAQRPADDEHRYGHERAEGVASLVVATIMGLASLEVGRGAVLALLSPERGAPAAWSLWVALGSAAILLAVYTYNLRLARHTGSKALEAAAYDHLSDFFISVGAAAGILGSQVGWRWADPLAGLLVAALIARTAWSIGSEAAHMLMDGFADRARIAALEEVVLGVQGVTGVQSLRARLMGSRVHVDVTVLVPSQMSIVEAHAVADRVEEALIRLSDVREVHVHVEPDVVAQAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB7</accession>
    <name>CH10_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-10</fullName>
            <shortName evidence="1">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groES</name>
        <name evidence="1" type="synonym">groS</name>
        <name type="ordered locus">STH2898</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197015.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KB7"/>
    <dbReference type="SMR" id="Q67KB7"/>
    <dbReference type="STRING" id="292459.STH2898"/>
    <dbReference type="PRIDE" id="Q67KB7"/>
    <dbReference type="EnsemblBacteria" id="BAD41881">
        <property type="protein sequence ID" value="BAD41881"/>
        <property type="gene ID" value="STH2898"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2898"/>
    <dbReference type="eggNOG" id="COG0234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132825_2_0_9"/>
    <dbReference type="OMA" id="RSINMTI"/>
    <dbReference type="OrthoDB" id="1965002at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000174874" description="Co-chaperonin GroES">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <sequence length="97" mass="10518" checksum="0C242F9E31FD8ED8" modified="2004-10-11" version="1">MNVKPLGARVVIKPLEKEERTKSGIVLPDTAKEKPQQGKVIAVGPGRTLESGTKVELEVKEGDTVIFSKYAGTEVKIDGEEYIILDGERDILAIVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC9</accession>
    <name>Q67KC9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2886</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197003.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2886"/>
    <dbReference type="EnsemblBacteria" id="BAD41869">
        <property type="protein sequence ID" value="BAD41869"/>
        <property type="gene ID" value="STH2886"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2886"/>
    <dbReference type="eggNOG" id="COG2414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020364_1_0_9"/>
    <dbReference type="OMA" id="HGCHRGC"/>
    <dbReference type="OrthoDB" id="332680at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.599.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013985">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AFOR_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="603" mass="65571" checksum="45FD727C0E29765A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI3</accession>
    <name>Q67KI3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2830</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2830"/>
    <dbReference type="EnsemblBacteria" id="BAD41815">
        <property type="protein sequence ID" value="BAD41815"/>
        <property type="gene ID" value="STH2830"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2830"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_242327_0_0_9"/>
    <dbReference type="OMA" id="DYWKYLY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032812">
        <property type="entry name" value="SbsA_Ig"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13205">
        <property type="entry name" value="Big_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269879" evidence="2">
        <location>
            <begin position="33"/>
            <end position="1625"/>
        </location>
    </feature>
    <feature type="domain" description="A2M_N_2" evidence="3">
        <location>
            <begin position="818"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="A2M" evidence="4">
        <location>
            <begin position="1013"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="983"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1625" mass="178327" checksum="BBA4A31C4B4D731E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK7</accession>
    <name>Q67KK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2806"/>
    <dbReference type="EnsemblBacteria" id="BAD41791">
        <property type="protein sequence ID" value="BAD41791"/>
        <property type="gene ID" value="STH2806"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2806"/>
    <dbReference type="HOGENOM" id="CLU_2276044_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="102" mass="11289" checksum="B41ECAD23E3F11D6" modified="2004-10-11" version="1">MADVQSYQKCLTDYCPLPHYRNWVPTPGGPGWQPGPTEGEGLGMAGFLVALLWLASILIFLWIWDDATRRHGQNIGCLWALAVLALGPIAIIAYFLFGRSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL4</accession>
    <name>Q67KL4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196918.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2799"/>
    <dbReference type="EnsemblBacteria" id="BAD41784">
        <property type="protein sequence ID" value="BAD41784"/>
        <property type="gene ID" value="STH2799"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2799"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_23_0_9"/>
    <dbReference type="OMA" id="HPRELIL"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="33751" checksum="AC8DA60CA1282D62" modified="2004-10-11" version="1">MRAPITLAVVGLGNIARKAHLPVLTSHPEVEIIGLSSRTGRNVDELAAQYRLRLRARTFAEILAARPRAAYLLSATPAHREQAIALMEAGIDVFMEKPLADNLVDAEAIAEAAGRTGRLLMVGFNRRYAPAYRRAKALFHDSGRRIEYVLVQKHRTGGGTRWPLRFYVTDDVIHIIDLARFFAGDLQLRTACARTGLVAAQMESADATLVQLSQSFSAGAVTERVELHGGGLTVIVEEMERLVVREDGAERVEPLFGSWTPTLQKRGIAPELEHFLACLREGRTPETSVAEALATQRLAEEILRAAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS2</accession>
    <name>Q67KS2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196859.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2740"/>
    <dbReference type="EnsemblBacteria" id="BAD41725">
        <property type="protein sequence ID" value="BAD41725"/>
        <property type="gene ID" value="STH2740"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2740"/>
    <dbReference type="eggNOG" id="COG1199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012117_2_0_9"/>
    <dbReference type="OMA" id="PRRAQNY"/>
    <dbReference type="OrthoDB" id="679382at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="31"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="656" mass="73011" checksum="E3F6F1D836DA1097" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX8</accession>
    <name>Q67KX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fructose-bisphosphate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2683</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196805.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2683"/>
    <dbReference type="EnsemblBacteria" id="BAD41668">
        <property type="protein sequence ID" value="BAD41668"/>
        <property type="gene ID" value="STH2683"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2683"/>
    <dbReference type="eggNOG" id="COG0191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040088_0_1_9"/>
    <dbReference type="OMA" id="NNMEIVQ"/>
    <dbReference type="OrthoDB" id="827430at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011289">
        <property type="entry name" value="Fruc_bis_ald_class-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01859">
        <property type="entry name" value="fruc_bis_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="209"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="2">
        <location>
            <begin position="230"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="30614" checksum="66C40CAC28E260DD" modified="2004-10-11" version="1">MTLVSLRTLLDAAQAGKYAVGAFNCNNMEVVQAIVAAAEEARSPVILQASQGAIKYAGLDYIVSMVKAAAAKATVPMALHLDHGTDFNTIMACIRAGFTSVMFDGSKYEKEENIARTKEIVKIAHAVGVSVEGEIGKIGGVEDDIAVDERDAMLTPVPDAIEFYEATGVDALAMSFGTAHGPYKLPPKLDLDRIAAVHQKTGAPLVMHGGSGVPEEDMRKGIELGIRKVNIDTELRQAFLAEVRRQLAERPDELDPRKIFAPAREVLKEHIKEKMRLFGCAGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ5</accession>
    <name>Q67KZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative endonuclease III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2666</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2666"/>
    <dbReference type="EnsemblBacteria" id="BAD41651">
        <property type="protein sequence ID" value="BAD41651"/>
        <property type="gene ID" value="STH2666"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2666"/>
    <dbReference type="eggNOG" id="COG2231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_9"/>
    <dbReference type="OMA" id="GPQGWWP"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="10">
        <location>
            <begin position="53"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28621" checksum="203EE35A30BA47E7" modified="2004-10-11" version="1">MVRPEPPEGWARWVAEVYRRLLEAFGPRHWWPSALEPPPAKAQPFEMIAGAILVQNVAWSNAAKAVRALAEAGLLDVAAMDAAPAEAVEPLIRPAAYFRQKAQRLKGFAAHVMNRYGGDLPAMLRRPADELRAELLALPGIGPETADCILCYAAGRPVMAMDAYTRRIFARLGVFAPDARYEAMQAFFHGWTPADAQLRGEYHALIDTLGNRLCLKRNPRCGQCPLADLCPRVGVVEEAGGAAGRARTVDGRGRGPCCEAGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ6</accession>
    <name>Q67KZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196787.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2665"/>
    <dbReference type="EnsemblBacteria" id="BAD41650">
        <property type="protein sequence ID" value="BAD41650"/>
        <property type="gene ID" value="STH2665"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2665"/>
    <dbReference type="eggNOG" id="COG1451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065947_1_0_9"/>
    <dbReference type="OrthoDB" id="1301886at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF45" evidence="1">
        <location>
            <begin position="87"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="21590" checksum="2DB379A886DBC186" modified="2004-10-11" version="1">MRIVFKDKKHSSGTIRDGEIVLYISSRLSRAEQVRHIEELTRRLTPRLERARQLQLTGAGPLSRLTPSPVTDDAALARWAREINDRYYGFPMGRVRFRRQESRWGSCSGRTRNIQISHRLRGGPHELLEYVLVHEIAHLGELNHSPRFWALVERACPDWRERRRLLRWYGEYLRAVGTPPDSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L47</accession>
    <name>Q67L47_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196736.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2614"/>
    <dbReference type="EnsemblBacteria" id="BAD41599">
        <property type="protein sequence ID" value="BAD41599"/>
        <property type="gene ID" value="STH2614"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2614"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_7_9"/>
    <dbReference type="OMA" id="KECINDV"/>
    <dbReference type="OrthoDB" id="1992113at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="31"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="137"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="22723" checksum="13AC8D73C5FB8160" modified="2004-10-11" version="1">MPEWDEQTIHRQLLARDPAGLDALAGALAHRLYRLAEMALGGLGSAQDAEEVVSDALAAAWERAAEFDPQRSTLANWVLMLTKYAALDRRRALRRRTLDDMGRAKVIPLSAVPEAAEPEETAPPVDAAVVAHEEQAAVHAALRRLPPAERELLIRRYFFEESVAEIARDLGLSRGALDTRLWRARQNLRRLLSDESEVHTHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L99</accession>
    <name>Q67L99_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative K(+)-stimulated pyrophosphate-energized sodium pump</fullName>
            <ecNumber evidence="9">7.2.3.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Membrane-bound sodium-translocating pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate-energized inorganic pyrophosphatase</fullName>
            <shortName evidence="9">Na(+)-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hppA</name>
        <name evidence="10" type="ordered locus">STH2562</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na(+) movement across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphosphate + H2O + Na(+)(in) = H(+) + Na(+)(out) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:57884"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Requires K(+) for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.3.-" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2562"/>
    <dbReference type="EnsemblBacteria" id="BAD41547">
        <property type="protein sequence ID" value="BAD41547"/>
        <property type="gene ID" value="STH2562"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2562"/>
    <dbReference type="eggNOG" id="COG3808">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008743_3_1_9"/>
    <dbReference type="OMA" id="MATTAMQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:pyrophosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01129">
        <property type="entry name" value="PPase_energized_pump"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="PTHR31998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001265">
        <property type="entry name" value="H+-PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="569"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="636"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="site" description="Determinant of potassium dependence" evidence="9">
        <location>
            <position position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="659" mass="66603" checksum="67BB1A62D01BB1A4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE8</accession>
    <name>Q67LE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196636.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2513"/>
    <dbReference type="EnsemblBacteria" id="BAD41498">
        <property type="protein sequence ID" value="BAD41498"/>
        <property type="gene ID" value="STH2513"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2513"/>
    <dbReference type="eggNOG" id="COG0029">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068376_1_0_9"/>
    <dbReference type="OMA" id="YVDHDVL"/>
    <dbReference type="OrthoDB" id="1324202at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="65"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="191" mass="19912" checksum="9AC706A55D906E6F" modified="2004-10-11" version="1">MYDVAIVGAGPAGASAATFTARAGLKTLLLDADQSMTRRAWIPNHLGFPDGVTGNDLVDLGKKQAQTAGANLVKAKVTAVRREGEGFRLETEGGEAYEARQVILCLGANCELARQAGAEIVEGPEPRIKEVVAVDREGRTRVEGLWAAGTCAGTSVHTIITAGDGARVAINLISAVKGARHVDHEVMPASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI8</accession>
    <name>Q67LI8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2473</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2473"/>
    <dbReference type="EnsemblBacteria" id="BAD41458">
        <property type="protein sequence ID" value="BAD41458"/>
        <property type="gene ID" value="STH2473"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2473"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043807_0_0_9"/>
    <dbReference type="OMA" id="RRFYQGI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015114">
        <property type="term" value="F:phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009887">
        <property type="entry name" value="ANKH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28384">
        <property type="entry name" value="PTHR28384"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="488" mass="52356" checksum="E6ED18E93BE94E8D" modified="2004-10-11" version="1">MPRRRGRWRPGRRGLDVPALQGTILVVDSRDRRDIAALTSTTESRRSAASGTLTYGTILRLFLPLSLSDVIMVIAGPILTIGLTKLANPEISLAAYAVANNVAILLESPIIMLLHASNLLSRYRETYQPLRHFMLWANALLTALYALLAFTPAYDLIFRTLLGQPDAIAAAARPAFQVQLLWPAAIGWRRFYQGILIQHKRSSVVAYAGFARIGSLALVTALGVMGRAHGATLAGLALVVSVIVEAAAVTWLARPVLQAGVTDPHAEAPDWAPRTVGQIALWYWPLAMTQILVSVVRPLLSGGIARSVDPELGLAAWPVAWSTILMVANAVRMVQQVALTLLQDRRSYLMLRRFTLTIGAVACCVMALLAVTPLGRGYMEWVLGLKGGLASVADAALPALGFGVVFPFYVALQNWLQALLIKDGRTLVVNAGAIAGGAVTLAAVYAGALIWRLPGAVLGIVSLLCGAAVELVVLVRASRPQRQAWLAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN1</accession>
    <name>DTD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <shortName evidence="1">DTD</shortName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gly-tRNA(Ala) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtd</name>
        <name type="ordered locus">STH2430</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DTD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LN1"/>
    <dbReference type="SMR" id="Q67LN1"/>
    <dbReference type="STRING" id="292459.STH2430"/>
    <dbReference type="EnsemblBacteria" id="BAD41415">
        <property type="protein sequence ID" value="BAD41415"/>
        <property type="gene ID" value="STH2430"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2430"/>
    <dbReference type="eggNOG" id="COG1490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076901_1_0_9"/>
    <dbReference type="OMA" id="GVFQAHM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043908">
        <property type="term" value="F:Ser(Gly)-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="PTHR10472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="SSF69500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00256">
        <property type="entry name" value="TIGR00256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000164609" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Gly-cisPro motif, important for rejection of L-amino acids" evidence="1">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00518"/>
        </source>
    </evidence>
    <sequence length="151" mass="16727" checksum="F4DB7724037E8157" modified="2004-10-11" version="1">MILRAVVQRVSRARVTVGGEVVGQIGPGYVVLLGVSREDDEAAADYLAEKVAGLRVFEDEEGKMNRSIQEAGGAVLAVSQFTLYGDVRRGRRPGFDRAARPEQAEPLYRRFVERLRALGLHVETGRFQTHMEVELVNDGPVTILIDSEKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN7</accession>
    <name>Q67LN7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diacylglycerol/lipid kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2424"/>
    <dbReference type="EnsemblBacteria" id="BAD41409">
        <property type="protein sequence ID" value="BAD41409"/>
        <property type="gene ID" value="STH2424"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2424"/>
    <dbReference type="eggNOG" id="COG1597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045532_2_1_9"/>
    <dbReference type="OMA" id="VTKSRYY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005218">
        <property type="entry name" value="Diacylglycerol/lipid_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00147">
        <property type="entry name" value="TIGR00147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="31473" checksum="18194A106D963485" modified="2004-10-11" version="1">MAFRRIAVIYNPASGRPREREEPLARLAAPLRAAGCEVAICPTARPGHATEIAQARAGEGYDLILAHGGDGTMNEALQGVMGTGAALGFWPGGTANVLAAELHLPHRPEEMARRIRDGRTAEVTVGRANDRYFLLMAGVGLDAAVAGSVDPELKRRFGKAAFFVAAMQFIWRWELAPFVVHLDGEPPVEGRFVVAGNARSYGGGFQFTPDASLTDPDLDVCIFTAEAVGDYLRYVSAALVGLHRNLPGVIYRKARRVSITAAAEHAPLTQVDGEVIGSLPVTLESIPRALRLLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS2</accession>
    <name>MNMA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="1">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmA</name>
        <name type="ordered locus">STH2389</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LS2"/>
    <dbReference type="SMR" id="Q67LS2"/>
    <dbReference type="STRING" id="292459.STH2389"/>
    <dbReference type="EnsemblBacteria" id="BAD41374">
        <property type="protein sequence ID" value="BAD41374"/>
        <property type="gene ID" value="STH2389"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2389"/>
    <dbReference type="eggNOG" id="COG0482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_0_0_9"/>
    <dbReference type="OMA" id="AVCTGHY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000349822" description="tRNA-specific 2-thiouridylase MnmA">
        <location>
            <begin position="1"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="1">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="1">
        <location>
            <begin position="131"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <sequence length="390" mass="43316" checksum="E2112223BD609F33" modified="2004-10-11" version="1">MPAGRRYGRTRRATLYVTKEGGGRPVAKRVLMAMSGGVDSSVAAALLVEQGYEVIGVTMNTWTDDIPEEIQMNQHSGCCSLAAVEDARSVAHKLGIPYYVMNFQGQFARTVIDYFIEEYTRGRTPNPCIACNRYVKFSAFLEKAKQLECDYVATGHYAVIGQDDRFPGRWLLGKSADARKDQTYVLHNLTQEALAHTLFPVGHLQKSEVRALAAKYGFVTADKPDSQEICFVYDNDYGRFLKERAPEAIVPGPILNTRGEVIGQHQGLPLYTIGQRKGLGLTTPRPVYVVDLDVERNAVIVGEDEETYRGGLVASDLNWIAIPGLTFPRRCRAKIRRMAPEAECTIYPIGEDAVRVEFDRPQRAITPGQAVVFYDGDWVLGGGTIERAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS8</accession>
    <name>Q67LS8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41368">
        <property type="protein sequence ID" value="BAD41368"/>
        <property type="gene ID" value="STH2383"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2383"/>
    <dbReference type="HOGENOM" id="CLU_1991541_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="125" mass="14426" checksum="367D8DBFCD52EC25" modified="2004-10-11" version="1">MSHSHVNFPSGPSVGIYERTWRERPVDEGKVFDQFLHLPDGYQAQVMVMAEPVSGLEGKVAYRSRVWLGHEDEMDPYLYRTEVHWDLQEAIRTCWAMLRQLAPHCFRSRAAIEAVKGVDRKLPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M07</accession>
    <name>Q67M07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196427.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2304"/>
    <dbReference type="EnsemblBacteria" id="BAD41289">
        <property type="protein sequence ID" value="BAD41289"/>
        <property type="gene ID" value="STH2304"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2304"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_9"/>
    <dbReference type="OMA" id="VGWVEPV"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="26477" checksum="DED261AA1FC7B43F" modified="2004-10-11" version="1">MGMLDGKVAIITGAAMGMGAATAELFAREGARVVIADLNEELGRAQARKIQDAGGAAAFVRADVSRAGDVEAMVQFAVDTFGRLDVAVNNAAHSPDTRPLADLDEAVWDAVIGVDLKGVALCLKYELRQMLKQGGGGSIINISSVSGIRPQPGTPAYVAAKHGVIGLTKQAAMDYSPHGIRVNAVAPGAVDTPMLQKALVEFNLEPVSYAKQLSLLGRFAQASEVAEVNLWLASDRSSYVTGAVYTVDGGYTAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M15</accession>
    <name>Q67M15_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2295"/>
    <dbReference type="EnsemblBacteria" id="BAD41280">
        <property type="protein sequence ID" value="BAD41280"/>
        <property type="gene ID" value="STH2295"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2295"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="296"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="552" mass="62143" checksum="1A80215C12D94574" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M47</accession>
    <name>Q67M47_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2263"/>
    <dbReference type="EnsemblBacteria" id="BAD41248">
        <property type="protein sequence ID" value="BAD41248"/>
        <property type="gene ID" value="STH2263"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2263"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_6_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="134" mass="15368" checksum="963AB0D277868EA0" modified="2004-10-11" version="1">MRTIFAQPDQKAARSQLAVVVENLRKQFPRAAQLLEDAEEDILAYMAFPSEHWRQLHSTNPLERLNREIGRRTEVVGIFPNREALIRLAGAVMIEQQEEWMTAPRRYFSQASMAKLYANDPSLSCPELLPLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA0</accession>
    <name>Q67MA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2208</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196333.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2208"/>
    <dbReference type="EnsemblBacteria" id="BAD41193">
        <property type="protein sequence ID" value="BAD41193"/>
        <property type="gene ID" value="STH2208"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2208"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_19_3_9"/>
    <dbReference type="OMA" id="LYCVQPM"/>
    <dbReference type="OrthoDB" id="1118058at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="10"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="402" mass="42320" checksum="65241CB56A12EBD9" modified="2004-10-11" version="1">MPRIQRGTREYQLVTLALFLSALSIYASLYVTQPILPLLSADFGIIPAQASLSVSTATISMAISQMLVGPVADSLGRKPIMTVAVLATGVIGLVASRTVAFGPFLFTRFLQGLVMAGLPATAMAYLAEEIDPQHLGAAMGLYISGNSLGGLGGRIISGSVAEFWGWRGAVAAIGVVSLVCALWFARALPPSRNFRAQPLQLRRLVHSLFGAVRDPLLRALYLVGFMAMGSFVALYNYVSYHLMAPPYSLSAALMGWIFLLYLAGTFSSALMGRLSDQYGRAPMLALSLGIELAGAAIALAGPLLVKIGGIAVFTFGFFGAHSIASSWVGQRARHDKGAASALYLFTYYMGASIAGTVAGLFWMRFGWPGVVGFIACLLATAVGLAALARKLSAGTETVSTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA1</accession>
    <name>Q67MA1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">XdhC_CoxI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2207"/>
    <dbReference type="EnsemblBacteria" id="BAD41192">
        <property type="protein sequence ID" value="BAD41192"/>
        <property type="gene ID" value="STH2207"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2207"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154250_1_0_9"/>
    <dbReference type="OMA" id="ETAWNVG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003777">
        <property type="entry name" value="XdhC_CoxI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02625">
        <property type="entry name" value="XdhC_CoxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="11207" checksum="36B96C14B544A08E" modified="2004-10-11" version="1">MTDVDDPILLLRASLAAGERVALATVVHTRGHCPREVGAKMLVWRDGRTAGSIGGGCGENRIRLAALTALDEGRPTLEQVDLLDDPSLPDGAVCGGVMEVLIEPFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD2</accession>
    <name>Q67MD2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nuclease SbcCD subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">sbcD</name>
        <name evidence="10" type="ordered locus">STH2176</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'-&gt;5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Heterodimer of SbcC and SbcD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the SbcD family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196301.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2176"/>
    <dbReference type="EnsemblBacteria" id="BAD41161">
        <property type="protein sequence ID" value="BAD41161"/>
        <property type="gene ID" value="STH2176"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2176"/>
    <dbReference type="eggNOG" id="COG0420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038045_3_0_9"/>
    <dbReference type="OMA" id="GNHDQHS"/>
    <dbReference type="OrthoDB" id="1831911at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004593">
        <property type="entry name" value="SbcD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00619">
        <property type="entry name" value="sbcd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 8 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="9">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="411" mass="45184" checksum="F8169C17B684750F" modified="2004-10-11" version="1">MRILHTADWHLGRTLEGRSRQEEHEAFVDELCAMVREERIDLVLIAGDVFDTGNPSAAAEELYCDALARLGENGRRAVVVIAGNHDSPDRLTAVQALAQRHGATMFGYPYDDPGVYTPGPDRVQRVAAGPGWAELTVPGVDHSAVVLALPYPSESRLRKLIADTLGEEELQRSYSDQVKGWLAAAAGRFRPDAVRLVTSHLYMAGGVESDVVERPIQMGGAYTVHPCAVPETAQYVALGHLHRPQEIHGTAAVTRYSGSPISFSFNETGYVKGVTVVEVEPRDPAPRVRHVPISAGRPLVRWVAEDGLAQVERWVAEGKDPTAWISLEVHTEHPLTLEQVHRLRAMRPEFVHIRAVLPAEAEAAATAEPQRLGVDEQFVRFYSQVRGGPPAPELVRLFLELVNARDEEVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH6</accession>
    <name>Q67MH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196257.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2132"/>
    <dbReference type="MEROPS" id="M23.009"/>
    <dbReference type="EnsemblBacteria" id="BAD41117">
        <property type="protein sequence ID" value="BAD41117"/>
        <property type="gene ID" value="STH2132"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2132"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_7_3_9"/>
    <dbReference type="OMA" id="WTWPANG"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269538" evidence="1">
        <location>
            <begin position="29"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="95"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="145"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="341" mass="35684" checksum="47320A83684756BA" modified="2004-10-11" version="1">MHLPTSALRRRVFATALAALVVSGVGVAARPHSAPATAPNQAGPDDATAGAIPEGFYGPALPVGAEAVALQKAAPAAAAQAQPEQEEEQSKPKVITYTVVEGDTIEDIAHRYGVSTETLLYINDMYADDILHIGQELLIPTMDALVYTVVEGDTMWGVADAFDADFDAIVAANPDVNADALQPGDLLFVPGGTPPSRRTMVASRGGSRTPAATGTFDIWPVWGGVTDWFGWRVHPVTGVWHLHDGIDLDAPTGTPVAAVASGTVTTAGWLGGYGYAVKVDHGDGIMTMYAHLSQVAVSVGEWVETGEIIGYSGSTGNSTGPHLHFTVLVWGEPVDPWGWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP6</accession>
    <name>Q67MP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ethanolamine utilization protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2062"/>
    <dbReference type="EnsemblBacteria" id="BAD41047">
        <property type="protein sequence ID" value="BAD41047"/>
        <property type="gene ID" value="STH2062"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2062"/>
    <dbReference type="eggNOG" id="COG4819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046255_0_0_9"/>
    <dbReference type="OMA" id="NCDYIDI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009377">
        <property type="entry name" value="EutA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06277">
        <property type="entry name" value="EutA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012293">
        <property type="entry name" value="EutA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="484" mass="50729" checksum="A1AC1192A718C41B" modified="2004-10-11" version="1">MRDSLTSIGIDIGTTSTCLVVSRLTTARLGGVHALASVEITGREVLYRSPVIFTPLMDEARLDGDAIFAWVREQLRRANLTWGSIDSGAVIITGESARRENARAVVERLSGDAGRFVVATAGPQLEAILAGRGSGAAALSREQNLRVLNLDVGGGTTNGALFVGGEVEATVCAHVGGRLVRVDPDTARITGVSPPARRAAEILGVRIEPGQPANPEALWTLCTGMAEALHELIGGRAESELARALAIGPPPPSPVRADVVTFSGGTGRELYAAAERRSLKEVTRYGDIGPMLADALRLGPTCRAFALREPPETVYATVIGAGMEVLELSGSTIFLHAEGLLPLHNVPMVRPPNLDPLAPAEAIAASVRRGLAWFETGGDGPDQPVAVAFPGLPHPRFEQVRNLAAGLAEGVRPLVERGLPVIVVLQENIGNVLGHSLAALLPPGHPVICVDQIRTQSGDYLDIGAPLYGGIVVPVVVKSLVFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS2</accession>
    <name>Q67MS2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="4">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2036"/>
    <dbReference type="EnsemblBacteria" id="BAD41021">
        <property type="protein sequence ID" value="BAD41021"/>
        <property type="gene ID" value="STH2036"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2036"/>
    <dbReference type="eggNOG" id="COG0520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_2_5_9"/>
    <dbReference type="OMA" id="HKLCGPT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="5">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="44552" checksum="422B20935C308BFB" modified="2004-10-11" version="1">MARIRQDFPILHQEMNGHPLVYLDNAATTQKPRQVIQALVEYYEGYNANVHRGIHTLAERATEAYEEARAKIARFIGSPSPNQVVFTRNGTEALNMIAYGWLRHRLRPGDVIVTSGMEHHSNLLPWQQAAQATGAQIKYIELLPDGTLNQEHYDRLLSEGNVRFVAFSGASNVLGTLNPVKEMVAKAHAVGARVAVDAVQVVPHMPVNVVDWDADWIAVAGHKMLAPTGTGFLWGKMELLEEMEPTYFGGSMISEAHLTGAKWAQPPAKFEAGTPNIADFIAFGAAVDYLADLGMENVYAHEKALTEYAWERLSAIDGMRLFGPRQPRAGLISFEVAGIHPHDLSAVLNAEGVAIRVGHHCAQPLMEILDVPATNRASFYIYNTQAEVDRLVESILHAKEFFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT9</accession>
    <name>Q67MT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2019"/>
    <dbReference type="EnsemblBacteria" id="BAD41004">
        <property type="protein sequence ID" value="BAD41004"/>
        <property type="gene ID" value="STH2019"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2019"/>
    <dbReference type="HOGENOM" id="CLU_2653159_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="76" mass="8375" checksum="D20833E58C012FEF" modified="2004-10-11" version="1">MDRLSTLTQTEPLTAHELLLLNELIRLESGDVQKTKSMLAMVSDADLRQQLTGCLQTGQAHVRALHDFCRARGIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N15</accession>
    <name>Q67N15_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3794 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40928">
        <property type="protein sequence ID" value="BAD40928"/>
        <property type="gene ID" value="STH1943"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1943"/>
    <dbReference type="HOGENOM" id="CLU_1631907_0_0_9"/>
    <dbReference type="OMA" id="VECVIPF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024300">
        <property type="entry name" value="DUF3794"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12673">
        <property type="entry name" value="DUF3794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3794" evidence="1">
        <location>
            <begin position="33"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="21451" checksum="86EDE297754276B1" modified="2004-10-11" version="1">MIQTPVVVNANTAQVLVVSEIPLTPPAFKIDHIDKLVEVDDCVAACDKVIINGRLIKNITYKTAKEWDHKGGLNRVCGDVRHCTVEIPFHLFIDVPGSRDGDDCEIEDAIVAGEFDKLVDRNRDGTFSKLLEKSVIKVRAKVVRRRWLKVNAQDVTPRRLRCPETSEVVTVPGLDIPTQDKGDKPFAPGDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N18</accession>
    <name>Q67N18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PTS system mannose/fructose-specific IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196067.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1940"/>
    <dbReference type="EnsemblBacteria" id="BAD40925">
        <property type="protein sequence ID" value="BAD40925"/>
        <property type="gene ID" value="STH1940"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1940"/>
    <dbReference type="eggNOG" id="COG2893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123235_0_1_9"/>
    <dbReference type="OMA" id="RNITCAS"/>
    <dbReference type="OrthoDB" id="2008912at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00006">
        <property type="entry name" value="PTS_IIA_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033887">
        <property type="entry name" value="PTS_IIA_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-4" evidence="3">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="132" mass="13582" checksum="ECBBE4CD9DC12D17" modified="2004-10-11" version="1">MIGIVLISHGPLASGLLQAAEMIAGEQSQVAVLELQPAQEMDQFREAMEQAVARVDSGDGVLIVADLFGGSPANTSAYLLRPGVEVVCGANLPMLLEVLTLRDGQTLSELAQTAVQAGASGALRLADVLGRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N37</accession>
    <name>GCSH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine cleavage system H protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvH</name>
        <name type="ordered locus">STH1921</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="1">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvH family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196048.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N37"/>
    <dbReference type="SMR" id="Q67N37"/>
    <dbReference type="STRING" id="292459.STH1921"/>
    <dbReference type="PRIDE" id="Q67N37"/>
    <dbReference type="EnsemblBacteria" id="BAD40906">
        <property type="protein sequence ID" value="BAD40906"/>
        <property type="gene ID" value="STH1921"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1921"/>
    <dbReference type="eggNOG" id="COG0509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097408_2_0_9"/>
    <dbReference type="OMA" id="YRDSHEW"/>
    <dbReference type="OrthoDB" id="1773485at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06848">
        <property type="entry name" value="GCS_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00272">
        <property type="entry name" value="GcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002930">
        <property type="entry name" value="GCV_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033753">
        <property type="entry name" value="GCV_H/Fam206"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017453">
        <property type="entry name" value="GCV_H_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11715">
        <property type="entry name" value="PTHR11715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01597">
        <property type="entry name" value="GCV_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00527">
        <property type="entry name" value="gcvH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0450">Lipoyl</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000119305" description="Glycine cleavage system H protein">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-lipoyllysine" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <sequence length="128" mass="14169" checksum="0061E1EF56E403D5" modified="2004-10-11" version="1">MANVPADLKYSKEHEWIRVEGNIGIVGITWFAQDQLGDVVFVELPEVGRELKQNEQFGVVESVKTVSDLYCPASGKVVEVNTKLESSPELINQDPYGEGWILKLELSNPAELDNLLDAAAYDAFTKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N65</accession>
    <name>Q67N65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1893</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196020.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1893"/>
    <dbReference type="EnsemblBacteria" id="BAD40878">
        <property type="protein sequence ID" value="BAD40878"/>
        <property type="gene ID" value="STH1893"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1893"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_2_2_9"/>
    <dbReference type="OMA" id="KINHAYR"/>
    <dbReference type="OrthoDB" id="491753at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269289" evidence="2">
        <location>
            <begin position="28"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="3">
        <location>
            <begin position="81"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="34311" checksum="7025EDFEF940B7C2" modified="2004-10-11" version="1">MRRFFAGALLGWILSLCALALGWRWFAAQHPPGTPAPGGSAGADSVQLGLVPEGQAEEPEGTAPAYVLVLGVDERPGDPGRSDTMLLVRVGGGPLRVLSLPRDTRVTLEGVGEAKLNAAYAWGGPELATRTVSELLGLPVEHYVKVNLAGFRRLVDLIGGVPMHIEAPMRYVDPDDGLVIDLRPGHQVLDGARAEQYVRFRNDAAGDDLSRIRRQQQFLRAAARRALQPANLPKLPALLRTAARYVDTNLPLTEQIRLATIAIRTYHEGTLVTETLPGYAAYVGPVSYFLPDTQEIQRLREAWLPPHAMDNAGASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N81</accession>
    <name>Q67N81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1877"/>
    <dbReference type="EnsemblBacteria" id="BAD40862">
        <property type="protein sequence ID" value="BAD40862"/>
        <property type="gene ID" value="STH1877"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1877"/>
    <dbReference type="eggNOG" id="COG4502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111510_0_0_9"/>
    <dbReference type="OMA" id="FNAKFRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010708">
        <property type="entry name" value="5'(3')-deoxyribonucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06941">
        <property type="entry name" value="NT5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="23947" checksum="75FD973F06F116F7" modified="2004-10-11" version="1">MARPILLFDLDGVCCNLMKKWLAVYNRDYHDNLRPEDITSWDWETFVKPECGKRIYHYLNRPGFFADLEPIEGCVESLGRLAAICELVVVTASPRQAAGDKIAWVRRHLPMVPKGNIVITHRKDLVRGDFMFDDAPKNLRNHPAIRILMDYPYNRHFHDCYRVHSWAEAEQLIHSLVARQQGRSRHPSAQWAMQPDHGDASGGDSSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA0</accession>
    <name>Q67NA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Stage III sporulation protein AE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spoIIIAE</name>
        <name evidence="3" type="ordered locus">STH1858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1858"/>
    <dbReference type="EnsemblBacteria" id="BAD40843">
        <property type="protein sequence ID" value="BAD40843"/>
        <property type="gene ID" value="STH1858"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1858"/>
    <dbReference type="eggNOG" id="ENOG502Z7PW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046838_1_1_9"/>
    <dbReference type="OMA" id="EYGGFLP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014194">
        <property type="entry name" value="Spore_III_AE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09546">
        <property type="entry name" value="Spore_III_AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02829">
        <property type="entry name" value="spore_III_AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269910" evidence="2">
        <location>
            <begin position="27"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="41009" checksum="8D833A060139F9ED" modified="2004-10-11" version="1">MRSVKRTWWWVLLLLAVALTALPASAEGPAAPGGLLWEQAAALDTSAIERFLDEVNRTWEGYGPQITLGDFLRLSSGEDSPSLSAGAILQGLLRYLVREVLANADLLMKLVVLAITAAVLGQMQSAMGADAAGSVAYWVIYLVLVGLAITGFGLAVGAARQVMESLNAFMLAVLPTLLTVLIALGGAATAAIFQPLMVTMLNVTSTVMVNVVFPLAFLAAVLDIVSGLHEKYRLSNLAHLLRQGATVTLGATGTVFLGTVAVKGAAGAVADGLSVKAAKFVTGSFVPVIGKTLADATDLIVGSSLLLKNALGMLGATAIFFIVAFPLLKILSISWVYQVAGALVQPAGAGEIARMLTTMAKSLYMLFAAVGMVALMFFISVVVIVGSANVAVMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA4</accession>
    <name>Q67NA4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195982.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1854"/>
    <dbReference type="EnsemblBacteria" id="BAD40839">
        <property type="protein sequence ID" value="BAD40839"/>
        <property type="gene ID" value="STH1854"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1854"/>
    <dbReference type="HOGENOM" id="CLU_962875_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="289" mass="30722" checksum="028842EF7C416578" modified="2004-10-11" version="1">MSPSFLQQLVDRLRQEPRVLAVYVDRGLTAPRTGEVQEITLHLAVEGSFAQAADAWAASLAELVYSGPESHGWTLITPDGTEWRLCFHGPSADFAEEGLQGVFDRRAPSRVREGAQEGASPSPGGAAGQAQTDVAAVAAAFWRDLYRAARAIRAGRALTAHHWLFACGGRMIDLYRLALQPGPSGRGWEGADEVPGMARALEPVREFLSSPLECPAQRRAARRLGEAFEGLVLPLCRRIGATYPMALRTLTFRALEPEPDAAGGGGAAPHPSDATARGRGSGSGSGMNV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC1</accession>
    <name>NADK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD kinase</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK</name>
        <name type="ordered locus">STH1837</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195965.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NC1"/>
    <dbReference type="SMR" id="Q67NC1"/>
    <dbReference type="STRING" id="292459.STH1837"/>
    <dbReference type="EnsemblBacteria" id="BAD40822">
        <property type="protein sequence ID" value="BAD40822"/>
        <property type="gene ID" value="STH1837"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1837"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_0_9"/>
    <dbReference type="OMA" id="VNLGHVG"/>
    <dbReference type="OrthoDB" id="1463423at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000229700" description="NAD kinase">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="142"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="183"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <sequence length="283" mass="29480" checksum="5968E48D7A6E2CC0" modified="2004-10-11" version="1">MPKYALVINEDKPMAVTTGEEILQRLEASGAAVLLHPAAAGRLGRPDLAAPEGPAWGEVDMLIVLGGDGTLIRAVQRVAPYGVPVLGINTGHLGFLTAMESGDALAELDRVLAGSYLLEERMMLEATVVRDGLALATMPALNDAVISKGPRARMVHLEVSVGETVVARYRADGVIVATPTGSTAYSLSAGGPVVEPTVDCLLVTPICPHTMSARSIVVGADVALAIRVAASPGEVGLSADGSDPFPLLPGDVVRVGRAPYTARLVRLPGYRFYDVLRQKLSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF8</accession>
    <name>Q67NF8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1800"/>
    <dbReference type="EnsemblBacteria" id="BAD40785">
        <property type="protein sequence ID" value="BAD40785"/>
        <property type="gene ID" value="STH1800"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1800"/>
    <dbReference type="HOGENOM" id="CLU_1089592_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021338">
        <property type="entry name" value="DUF2953"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11167">
        <property type="entry name" value="DUF2953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28194" checksum="B6EBE6B0C69CBED6" modified="2004-10-11" version="1">MGNGPPPLWQDSPRQCGKGFRGWVKMGWLIAVGCLAAAMALLWLAPVQLHLRLAQANLKAEVFVELRVGWLRFARAITLGEKAPRVRARTRRRPGAKKLDVRDLLAASVQGLRYLGRATWCERLRLRVEVGGLDACDSALLAGLGWSLVCNLLAQFGRLVQVNPAGVAVAVVPNFQRPLLRTDLDCILRVPLGKATWAAGMVLRAALRRRALLARIRERQRRKGDRTDGRSPDSSPHEDGDGQPEEHGGREHGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG9</accession>
    <name>Q67NG9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40774">
        <property type="protein sequence ID" value="BAD40774"/>
        <property type="gene ID" value="STH1789"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1789"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808727_0_0_9"/>
    <dbReference type="OMA" id="PSERVWE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269913" evidence="2">
        <location>
            <begin position="23"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="3">
        <location>
            <begin position="135"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="37126" checksum="C8D8E5DDC7E5F272" modified="2004-10-11" version="1">MAGALALLLAALALPWGGRRPAADNGALRVAQGVTLLGFDFSGMTEREAKALLGELATGYRALPVSARRYESADGIAYTVPELNGYELDVERSWLRLATAPPGTAVEPAVQVQPAETRLADLPLGAIRQGNSEKQAVALLINVDWGTEELTRMLPILKRKDARATFFLSGRWAEQNPNLTRLIAADGHEVATHGHNLTHGPKALARAGRLKDDIAQSVRVIEEITGRKVKYYAPHKAEVDQAVLQTAADLNLQTVLYSLDTVDWNEQYATPARILETFQKAKAGDLILMHPKPNTVQVLEQAIDLLQSRGYRLVTLSEMLSPDPLPPGATGLQPPEEGTGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH2</accession>
    <name>Q67NH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore maturation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195914.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1786"/>
    <dbReference type="EnsemblBacteria" id="BAD40771">
        <property type="protein sequence ID" value="BAD40771"/>
        <property type="gene ID" value="STH1786"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1786"/>
    <dbReference type="eggNOG" id="COG0700">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127717_0_0_9"/>
    <dbReference type="OMA" id="VSTMQGS"/>
    <dbReference type="OrthoDB" id="1757687at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="46"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="18638" checksum="36671B48570ABF44" modified="2004-10-11" version="1">MSLLDGLSLWAVPGMLALIPLWGAIRRVPVYEAFVEGAEEGLEIAVKIIPFLVGMLTALSIFRASGAMELLASALSGVTGALGFPPEVLPLMIVRPLSGAGALAVTADLLKTHGPDSFIGRLASIMQGSTDTTFYVLTVYFGSVGIRRVRYSAAVGLTADLVGFLAALYVCQWFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI6</accession>
    <name>Q67NI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1772"/>
    <dbReference type="EnsemblBacteria" id="BAD40757">
        <property type="protein sequence ID" value="BAD40757"/>
        <property type="gene ID" value="STH1772"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1772"/>
    <dbReference type="eggNOG" id="ENOG503384K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183618_0_0_9"/>
    <dbReference type="OMA" id="AKWIFSK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="11398" checksum="E9D3EC1EC0EE70E2" modified="2004-10-11" version="1">MGYDEVQALRDRIRALEERTRLLEERIRGLRTSRRILMNLLAAQERERRALVARLEAENGRLQRKTARFARAVLERNIRIVRLEESLRRQTDVSSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ5</accession>
    <name>MTAD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-methylthioadenosine/S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="1">MTA/SAH deaminase</shortName>
            <ecNumber evidence="1">3.5.4.28</ecNumber>
            <ecNumber evidence="1">3.5.4.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtaD</name>
        <name type="ordered locus">STH1703</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of 5-methylthioadenosine and S-adenosyl-L-homocysteine into 5-methylthioinosine and S-inosyl-L-homocysteine, respectively. Is also able to deaminate adenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'-thioinosine</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.28" evidence="1"/>
    <dbReference type="EC" id="3.5.4.31" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195831.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NQ5"/>
    <dbReference type="SMR" id="Q67NQ5"/>
    <dbReference type="STRING" id="292459.STH1703"/>
    <dbReference type="EnsemblBacteria" id="BAD40688">
        <property type="protein sequence ID" value="BAD40688"/>
        <property type="gene ID" value="STH1703"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1703"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_9"/>
    <dbReference type="OMA" id="IIASWGK"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
        <property type="term" value="F:5'-methylthioadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000312459" description="5-methylthioadenosine/S-adenosylhomocysteine deaminase">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <sequence length="436" mass="46967" checksum="60E6371CE58DC4CC" modified="2004-10-11" version="1">MERLVIEGGTVLPMTGQADVYENGVVLVEAGRIVYAGPRDGAPHLAGARRIDASGRIVMPGIVNTHCHAAMTLLRGYADDMRLMEWLQTKIWPAEARMTADDVYWGTALGAYEMLSGGITTFLDMYFPADAVARAIQDTGIRGIVARGIIGVGGPSEALSRLDESREAFHRWNGKAGGRITFMVGPHAPYTCPPDALQACAELADELGVGIHIHLSETRDEVEEARRNWGKSPIRHVYDLGLMKGRHVVAAHCVHVDDDDIAILAETGTGVCHCPVSNLKLASGRTPVAKMRRKGVAVGFGTDGASSENMLHILGSEMRIGAIQAKELEGDPAVYTAYDAVAMATIEAARVLGMESEIGSLEPGKKADLILIDAERPHLTPNHDVFALIAYSALPGDVVMTIVDGRIVYEDGRLTTMDGREIMARVREAAARLVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB3</accession>
    <name>Q67PB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195625.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1495"/>
    <dbReference type="EnsemblBacteria" id="BAD40480">
        <property type="protein sequence ID" value="BAD40480"/>
        <property type="gene ID" value="STH1495"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1495"/>
    <dbReference type="HOGENOM" id="CLU_2557041_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="8970" checksum="07CC66C2CA64B806" modified="2004-10-11" version="1">MESGSWRWDLVGLRWEEAQAILQDRGVAYTWSVTAPPNRPVGIGELRVVAQRETPAGPAFVLAHREYARREQPAGQGGQGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC1</accession>
    <name>Q67PC1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1487"/>
    <dbReference type="EnsemblBacteria" id="BAD40472">
        <property type="protein sequence ID" value="BAD40472"/>
        <property type="gene ID" value="STH1487"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1487"/>
    <dbReference type="eggNOG" id="COG1315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026157_2_1_9"/>
    <dbReference type="OMA" id="TGHVKFK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005646">
        <property type="entry name" value="FapA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38032">
        <property type="entry name" value="PTHR38032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03961">
        <property type="entry name" value="FapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="476" mass="50162" checksum="29F9E8FD5FCA0F96" modified="2004-10-11" version="1">MSEPREGLDAGRTGPRDAELLLLVSPNAMSVEARVVPPEPGGRNITREQVLQELARLKVVFGIDHAAIDRLVEMAEKGFRLDRQGIVVAEGRPPVDGQDAAIIHHPLLQTPAGYPRLTEDGRADFFDLNLVRNVAKDTVLATRKPPTPGEPGIDVFGRTVKAREGRDVRLRAGAGTRLSPDGQSVIATVEGHASISASGEVTVSPIFTVPGDVGYGSGNIDFVGTVVVRGDVTQGFTVKAGGNVEVHGGIMGGSMEAGGDVLVRYGIVGAGRGRVKAGGKVRCRFIESAEVEAAGDVVVSDGILNSQVSGDSVLVTGGRGSIIGGRIRARREISAQTLGSPMGAATDLQVGVPPAVRAELDQIREQLARVEDRFGQATRTATYLNSGAAETARISREFAEKSRRLTGAERQQLAGRLAALQSQLEPEPGACVKGYEAVYPGVRITIGAQRHVVVDQSTNSCFVIGEDGEIRLVPAW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD3</accession>
    <name>Y1475_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0102 protein STH1475</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1475</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0102 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195605.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PD3"/>
    <dbReference type="SMR" id="Q67PD3"/>
    <dbReference type="STRING" id="292459.STH1475"/>
    <dbReference type="EnsemblBacteria" id="BAD40460">
        <property type="protein sequence ID" value="BAD40460"/>
        <property type="gene ID" value="STH1475"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1475"/>
    <dbReference type="eggNOG" id="COG0792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115353_2_3_9"/>
    <dbReference type="OMA" id="RNWRCGR"/>
    <dbReference type="OrthoDB" id="2044249at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00048">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34039">
        <property type="entry name" value="PTHR34039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00252">
        <property type="entry name" value="TIGR00252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000336271" description="UPF0102 protein STH1475">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00048"/>
        </source>
    </evidence>
    <sequence length="118" mass="13011" checksum="D525C2C56A6FFDEC" modified="2004-10-11" version="1">MSRRVGEAGEQAAAEFLTASGYRIIARNVRFRSGEIDLIAQDGGVLVFVEVKTRRGRRYGTPGEAVTAAKQRRLARLASLYLARLGSEPPPCRFDVVEVEPGPDGRLRCRLIQNAFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE0</accession>
    <name>Q67PE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195598.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1468"/>
    <dbReference type="EnsemblBacteria" id="BAD40453">
        <property type="protein sequence ID" value="BAD40453"/>
        <property type="gene ID" value="STH1468"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1468"/>
    <dbReference type="eggNOG" id="ENOG50301P4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135539_0_0_9"/>
    <dbReference type="OMA" id="WKEEVQQ"/>
    <dbReference type="OrthoDB" id="1976442at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021297">
        <property type="entry name" value="YlqD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11068">
        <property type="entry name" value="YlqD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14875" checksum="308C8703BD4E7E18" modified="2004-10-11" version="1">MSLQILRPVTIKARVTESLKARLTAELNAAIKQLDDEMAELESQVKRAQLTATISPQQQLQLHQLVEQERAVRAEKKAQLQEEIRRVQALPLGAEVVQGTIQATATVKVGDSLDALMTAEIVVEDGKVIEIRGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF4</accession>
    <name>Q67PF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoVS</name>
        <name evidence="1" type="ordered locus">STH1454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195584.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1454"/>
    <dbReference type="EnsemblBacteria" id="BAD40439">
        <property type="protein sequence ID" value="BAD40439"/>
        <property type="gene ID" value="STH1454"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1454"/>
    <dbReference type="eggNOG" id="COG2359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168904_1_0_9"/>
    <dbReference type="OMA" id="IPAFTEI"/>
    <dbReference type="OrthoDB" id="1985350at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007347">
        <property type="entry name" value="SpoVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35331">
        <property type="entry name" value="PTHR35331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04232">
        <property type="entry name" value="SpoVS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="86" mass="8800" checksum="66234C68690BEC78" modified="2004-10-11" version="1">MEALKVSATSNPKSVAGALAAVLREKGSAEVQAVGAGAVNQAVKAIAIARGYVAPNGIDLITIPAFAEIAIEGEERTAIRFIVEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG6</accession>
    <name>Q67PG6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1442"/>
    <dbReference type="EnsemblBacteria" id="BAD40427">
        <property type="protein sequence ID" value="BAD40427"/>
        <property type="gene ID" value="STH1442"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1442"/>
    <dbReference type="eggNOG" id="COG1399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100236_1_1_9"/>
    <dbReference type="OMA" id="RLDHRNP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="23301" checksum="6381F07B85037A31" modified="2004-10-11" version="1">MLTRKGMRRYNTACARAARPGAGPGLGWFPVRIDVADIKQEVGSHKLTDLSVTLDSAEFGGAEVRFDRPFTGKAKIWNLGDRLLVQAELQGEVRLTCSRCLREYTQPVSVSFEEEFREGEPGAEGPDEEDGDESVEPVSFYSDDELDVTEVARDHILLALPMKPLCSEACQGLCPRCGKDLNEGPCGCGGAEENVDPRLAVLKDLLRKPDSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH2</accession>
    <name>Q67PH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein, glycine-rich</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40421">
        <property type="protein sequence ID" value="BAD40421"/>
        <property type="gene ID" value="STH1436"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1436"/>
    <dbReference type="HOGENOM" id="CLU_2511478_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="85" mass="9369" checksum="0237BE067B09C16F" modified="2004-10-11" version="1">MILRKVREEGGFRVRLGLRLGLRLGLRLGLRLGLRFGLRIGIRHGSGRCAGFRVGVVIGRLIGQRRCVGRRLRPGGVSGRRGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL5</accession>
    <name>Q67PL5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hemerythrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the hemerythrin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1393"/>
    <dbReference type="EnsemblBacteria" id="BAD40378">
        <property type="protein sequence ID" value="BAD40378"/>
        <property type="gene ID" value="STH1393"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1393"/>
    <dbReference type="eggNOG" id="COG2703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086902_3_1_9"/>
    <dbReference type="OMA" id="MLWKDKY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12107">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016131">
        <property type="entry name" value="Haemerythrin_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035938">
        <property type="entry name" value="Hemerythrin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012827">
        <property type="entry name" value="Hemerythrin_metal-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47188">
        <property type="entry name" value="SSF47188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02481">
        <property type="entry name" value="hemeryth_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00550">
        <property type="entry name" value="HEMERYTHRINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin" evidence="4">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="17166" checksum="79B160D2838AC56C" modified="2004-10-11" version="1">MEELCFFTAGGKEMALRWYSALETGIEEIDGQHRQLFEQIDRLLESAKSGAGQDACLRILDFLGGYVVEHFATEERYMERYGYPKKAEHKQQHAELVEYYRKMRARVEAEGAKPTLIIQLQHSLVEWLNNHILKSDRDLGDFLKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN6</accession>
    <name>Q67PN6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195502.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1372"/>
    <dbReference type="EnsemblBacteria" id="BAD40357">
        <property type="protein sequence ID" value="BAD40357"/>
        <property type="gene ID" value="STH1372"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1372"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_9"/>
    <dbReference type="OrthoDB" id="955232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="35074" checksum="C65FB22B7044BBA9" modified="2004-10-11" version="1">MSRRALITGVTGVLGRELAGCLRRRGYAVYGAARRPLEGWQDGLDWLPIECDVTDDASVLRAIRLSRPELIFHLAAAQASATRSPSVDRLFAVNVGGTLRLLTAAAEAAPGARVIVASTGAVYGPNTGPRHRWREDAPLRPPSPYAASKAAMELAARGAAAGLGVEVVVARLFNLIGVSPGADSAPAGFARQIAAMAAAGRPRPLRTGNLDAVRDYLDSRDAARALTLLGELPDPDPVYNVCSGRGVRLRWVARQLWRLGGAGAPFLAEGDIGTAKLPGGAGSHPPDRLVGDPGRIRAATGWAPEVPLTRSLERLLAYWQTRATAGREEERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP7</accession>
    <name>Q67PP7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DhaL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195491.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1361"/>
    <dbReference type="EnsemblBacteria" id="BAD40346">
        <property type="protein sequence ID" value="BAD40346"/>
        <property type="gene ID" value="STH1361"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1361"/>
    <dbReference type="eggNOG" id="COG1461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017496_1_0_9"/>
    <dbReference type="OMA" id="TRTIPQG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019986">
        <property type="entry name" value="DAK2_dom-cont_prot_YloV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033470">
        <property type="entry name" value="UPF_DhaK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13684">
        <property type="entry name" value="Dak1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01121">
        <property type="entry name" value="Dak1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="SSF101473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03599">
        <property type="entry name" value="YloV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DhaL" evidence="1">
        <location>
            <begin position="9"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="583" mass="62756" checksum="CAB00E4E8BC8B3E4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS4</accession>
    <name>Q67PS4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4261 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195465.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1334"/>
    <dbReference type="EnsemblBacteria" id="BAD40319">
        <property type="protein sequence ID" value="BAD40319"/>
        <property type="gene ID" value="STH1334"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1334"/>
    <dbReference type="eggNOG" id="ENOG502ZA5G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1029390_0_0_9"/>
    <dbReference type="OMA" id="YEIMAYK"/>
    <dbReference type="OrthoDB" id="609567at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025357">
        <property type="entry name" value="DUF4261"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14080">
        <property type="entry name" value="DUF4261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4261" evidence="1">
        <location>
            <begin position="180"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="271" mass="29334" checksum="8D9518704E3494B6" modified="2004-10-11" version="1">MGDQSSLLLASLLLREPVMPDPAAVSARLAARCGGRYTVSWDEAEGEGDITLSCSVSGHTVVLGLMRAPVPAADLEAACARNPFWPEAAEVCRQHRAHLMVTLMKGWGDPIRRHLVLTDFVAALSEAVGGLAVLWGPVGVLQSAEYFREQAAEASADHLPLFLWVEFALVRHDGVPFVATYGLDAFGVMEVEGGSSRMKPIQLLERVFDVAHYLCLEGPVLNDGDTIGGSERERIPVRHTDSILDRPGPVIRIEFDAAGGRRGLLSRLFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT5</accession>
    <name>Q67PT5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1323"/>
    <dbReference type="EnsemblBacteria" id="BAD40308">
        <property type="protein sequence ID" value="BAD40308"/>
        <property type="gene ID" value="STH1323"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1323"/>
    <dbReference type="eggNOG" id="ENOG502ZFQS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2235205_0_0_9"/>
    <dbReference type="OMA" id="AGFCENA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="105" mass="12427" checksum="18A0C6ABB6EB6640" modified="2004-10-11" version="1">MQNRRAHMYEFQGRDWTELARAWGISLEHEDDELAARVRHYMRTHVSADATPDPAMVADLRRFVAGFCENAKERPDAPLWQGLRDIQHDLTFVQFCDVLLRHMWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY0</accession>
    <name>Q67PY0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PTS system mannose-specific IID component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1278</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1278"/>
    <dbReference type="EnsemblBacteria" id="BAD40263">
        <property type="protein sequence ID" value="BAD40263"/>
        <property type="gene ID" value="STH1278"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1278"/>
    <dbReference type="eggNOG" id="COG3715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036800_0_0_9"/>
    <dbReference type="OMA" id="DIMAIAI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004700">
        <property type="entry name" value="PTS_IIC_man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004704">
        <property type="entry name" value="PTS_IID_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03609">
        <property type="entry name" value="EII-Sor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03613">
        <property type="entry name" value="EIID-AGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51106">
        <property type="entry name" value="PTS_EIIC_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51108">
        <property type="entry name" value="PTS_EIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="484" mass="51682" checksum="55CDCFF9E7C9E525" modified="2004-10-11" version="1">MGDLHTGLVVGGTLEFVFMGAVDIGGSVPPNYAIGAVLGTAFAIATGQGVETALLIAVPAALLGSFFEVLAKTFSSFFVNAAERAADRGDDRSIAMFMHLGNLLHFLAYALPTFIALALGASAVQRLAASIPPWLNSGISVAGKMLPALGFALLLNSLAPGTMLPFFFVGFLLAAYTSWGVLGIAVLAILIALIIQHYRQANDEDAAELDPEATAGLGDTITRGDLRTLFFRSFALQSAFSFDRMQALGWTWSLIPFLKKIYRDQPEEYKNALKRHLTFFNTHMWVHGPILALTADLEARRAHGEDIDEQAIQGLKGSLMGPLAGLGDSMFHGTLRPIMGGICAGLAVTGNPLAPIIFFVTVNAVHVFCAWYGLYKTYEWGQTVIARLASGGLRQFLEGASIAGLMAVGALTGTWLNVTTPLAYKVQEATVSVQGMLDSIMPRILPLTLVMIVFWFVRKQKKTTNIMLGLIAAGLVLGSLGVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ3</accession>
    <name>Y1265_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0145 protein STH1265</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH1265</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195396.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PZ3"/>
    <dbReference type="SMR" id="Q67PZ3"/>
    <dbReference type="STRING" id="292459.STH1265"/>
    <dbReference type="EnsemblBacteria" id="BAD40250">
        <property type="protein sequence ID" value="BAD40250"/>
        <property type="gene ID" value="STH1265"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1265"/>
    <dbReference type="eggNOG" id="COG0393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_3_2_9"/>
    <dbReference type="OMA" id="VDFEISM"/>
    <dbReference type="OrthoDB" id="2015368at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="PTHR34068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225850" description="UPF0145 protein STH1265">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00338"/>
        </source>
    </evidence>
    <sequence length="104" mass="11039" checksum="C03004077BD20B10" modified="2004-10-11" version="1">MIVTTTPSVEGRQIAEYLGIVSAEAIMGANFFRDLAASITDLIGGRAGSYESKLREGREECLRELVAEAARLGADAVVGVDIDYEVVGESMLMVTASGTAVRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q05</accession>
    <name>Q67Q05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase, short-chain specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195384.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1253"/>
    <dbReference type="EnsemblBacteria" id="BAD40238">
        <property type="protein sequence ID" value="BAD40238"/>
        <property type="gene ID" value="STH1253"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1253"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_9"/>
    <dbReference type="OMA" id="QVMQGFD"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="229"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="387" mass="41963" checksum="D919CD7BDF7BD13C" modified="2004-10-11" version="1">MQFELSPEQQELVRLARQVGRDVIAPRAAQVDAEGVYPEDYFQAFKETGLLGVAIPEEYGGAGLGCLGLVLAVEEVAKHCCTAGLMLLLTRLSTMAISLAGTEAQKREYLEGTATGRRRGAFCLSEPDAGSDAQAIRTRAVRKGDRYVIQGTKSWISGAGAADFFVVAAKTRTDVPGARGFSVFVVDRDTPGVTVGRKEHKMGVRGLPVHQVIFEDVEVPVSSLVGQENEGFKLIMQNLNAVRPVVAARGLGLAEGALMYWLEYARTRQTFGRPLIEHQGLQWMAAELAAEIEACRLLTYRAASLVDQGRVGREAAHFLSMAKLKATELAVKAANDCLQMMGAVGYMEDYPMARFVRDARQLTIVEGTSQIQKGIIGRALADGLLAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q85</accession>
    <name>Q67Q85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1173"/>
    <dbReference type="EnsemblBacteria" id="BAD40158">
        <property type="protein sequence ID" value="BAD40158"/>
        <property type="gene ID" value="STH1173"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1173"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1721431_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="17025" checksum="16716550BE88E725" modified="2004-10-11" version="1">MVALAASALPLDPLAYVQTASVVSHILQTRCDGYLKRTHGLSWPGFTVLRTLKQCGPTSAKRLTAALACTHSNITGIVDRLERDGWVRRTRSTEDRRVIYLELTEKGERIDEVEEGLHRFLTLSLGHWFGEAHPLFERLQALEARMGDGQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE1</accession>
    <name>Q67QE1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1117</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1117"/>
    <dbReference type="EnsemblBacteria" id="BAD40102">
        <property type="protein sequence ID" value="BAD40102"/>
        <property type="gene ID" value="STH1117"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1117"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_26_0_9"/>
    <dbReference type="OMA" id="EHGVHFF"/>
    <dbReference type="OrthoDB" id="1465613at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="2">
        <location>
            <begin position="58"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="3">
        <location>
            <begin position="227"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="427" mass="45687" checksum="49EDCAE7397E25D4" modified="2004-10-11" version="1">MASPSLSPWRNRAAGDGANPSLSPWRTRAAGDGASPSLSPWRNRAAGDGAISSRRPWRIGLAGAGAFGRFVLTALAPLPDVELAAVAARSEASRAAALAAWRAAREVAGLPPGAPEAVDDAVALAQRPDLDAVLVATPPDLQPQVAGAAIAAGKALFLEKPGALAAEPLRELALRARSRGVVAALNFVMRASPLIACVRRWYRAGLLGLPERWHVENWAGGELPPDHWFWDRSRSGGVLIEHGVHFFDEAAWVLGGRPVRAWGADWPHPRHPDAAARALAAVEYAGELDGRPWHLAATFYHGFVRRRDEHQVRELGFTAGWLRIEDWIPRRLTGVLRVEPEAARALQEAAADLVRVEVRPLPDGRTWVEAAVAGGEATYREMIREGFVRLRRAAEGEGEPLAPLTAGVAALQVAEAASAGGWRAVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH6</accession>
    <name>Q67QH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1082</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195214.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1082"/>
    <dbReference type="EnsemblBacteria" id="BAD40067">
        <property type="protein sequence ID" value="BAD40067"/>
        <property type="gene ID" value="STH1082"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1082"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="SEIWSET"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="208"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="308"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="505"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="622" mass="66257" checksum="839A360F9D75CD74" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH9</accession>
    <name>Q67QH9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endo-1,4-beta-glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1079</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195211.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1079"/>
    <dbReference type="EnsemblBacteria" id="BAD40064">
        <property type="protein sequence ID" value="BAD40064"/>
        <property type="gene ID" value="STH1079"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1079"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_9"/>
    <dbReference type="OMA" id="MHTPVEM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="37973" checksum="407719DC4252FC99" modified="2004-10-11" version="1">MDITQHLAELTRLSSITGRERAVAEYLLQAWAPLVDEAYLDRLGSYVGVKRGEGPEPRPRVLAAAHIDSIGGVVTAIEPGGFIRFNPVGGVDRRLLMAQEMEVHGRRVIPGVVGSKPPHVTSPEERKRLVPVEELYIDTGLPEDEVRALVQVGDPVLFRYQFRILQNGRISSRYLDNRASQAALAVALQELQGLRHSADFYAVGTVGEEFGAYAGATAAAFALQPDIAIAVDVTFARHPGAEKDTFPLGGGPAIGVGPSCTPQVVKLMRKVAEEVGIDYQVEVMAGPSGTDAWGMQEVRGGVATAILSIPLRYMHTPVELVDLADIRQAGRLLAHVVARIDQAFVEELRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW4</accession>
    <name>Q67QW4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195076.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH944"/>
    <dbReference type="EnsemblBacteria" id="BAD39929">
        <property type="protein sequence ID" value="BAD39929"/>
        <property type="gene ID" value="STH944"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH944"/>
    <dbReference type="eggNOG" id="COG3011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086500_5_0_9"/>
    <dbReference type="OMA" id="KVVPSYW"/>
    <dbReference type="OrthoDB" id="2019103at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007263">
        <property type="entry name" value="DCC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04134">
        <property type="entry name" value="DCC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="14145" checksum="F5334C4655245AD5" modified="2004-10-11" version="1">MQPITVFYDGWCPLCTAARQRLARLDWLGRLEFVSIRDPGVAERAGVAPERLAARLHVHTPRTGRWAEGIWAVAAIAARLPLLWPLWPLLALAGVTGLGQPLYDFIARRRAIVPVGQCDETGCPLPGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX7</accession>
    <name>Q67QX7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH931"/>
    <dbReference type="EnsemblBacteria" id="BAD39916">
        <property type="protein sequence ID" value="BAD39916"/>
        <property type="gene ID" value="STH931"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH931"/>
    <dbReference type="eggNOG" id="ENOG503343A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2235204_0_0_9"/>
    <dbReference type="OMA" id="MIEREHY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="105" mass="11784" checksum="F42708D5218613CF" modified="2004-10-11" version="1">MQHTHPEVIAMNPEELSATCQYIISELGRIETVAGTLAMIEREHYDALNRFDDRALLDLATEEQSAARQLSMVKHVCGELARRMADIQSALERRPEGGEDRAPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R02</accession>
    <name>Q67R02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acylaminoacyl-peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH906</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195038.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH906"/>
    <dbReference type="ESTHER" id="symth-q67r02">
        <property type="family name" value="ACPH_Peptidase_S9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39891">
        <property type="protein sequence ID" value="BAD39891"/>
        <property type="gene ID" value="STH906"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH906"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_2_1_9"/>
    <dbReference type="OMA" id="KENDWIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="4">
        <location>
            <begin position="506"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="717" mass="79617" checksum="F1ACC8E3418BB611" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R19</accession>
    <name>Q67R19_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH889"/>
    <dbReference type="EnsemblBacteria" id="BAD39874">
        <property type="protein sequence ID" value="BAD39874"/>
        <property type="gene ID" value="STH889"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH889"/>
    <dbReference type="HOGENOM" id="CLU_1331360_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="22643" checksum="6106578F1198E445" modified="2004-10-11" version="1">MSLNFLREARVLFHGRRGLAWLVPLAVLLLRFAGFDATSPENAAALELLLPLVGPAIAVVLLSLDQHGMGEVVLAGSRAGHFVWVYRLAWVVLWLLLLVAVPAPSPDLLLALGPAALLMGVVLSLSRRISLRAGLSVALVWWGISYLAIRLADPLLYFSPFSWAMLQLVQTGLFPEEIWLRKGAQLLLGLLLALDWARQNMKGHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R28</accession>
    <name>Q67R28_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195012.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39865">
        <property type="protein sequence ID" value="BAD39865"/>
        <property type="gene ID" value="STH880"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH880"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="ILDRDNM"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49956" checksum="9EB05AD284E4720B" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRCLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEANRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R45</accession>
    <name>Q67R45_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredox_DsbH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39848">
        <property type="protein sequence ID" value="BAD39848"/>
        <property type="gene ID" value="STH863"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH863"/>
    <dbReference type="eggNOG" id="COG1331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014051_4_1_9"/>
    <dbReference type="OMA" id="PFYFGTY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004879">
        <property type="entry name" value="DUF255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024705">
        <property type="entry name" value="Ssp411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42899">
        <property type="entry name" value="PTHR42899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03190">
        <property type="entry name" value="Thioredox_DsbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006402">
        <property type="entry name" value="UCP006402_thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredox_DsbH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="623" mass="69856" checksum="EC6EA892E185B5FC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R55</accession>
    <name>Q67R55_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH853</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH853"/>
    <dbReference type="EnsemblBacteria" id="BAD39838">
        <property type="protein sequence ID" value="BAD39838"/>
        <property type="gene ID" value="STH853"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH853"/>
    <dbReference type="HOGENOM" id="CLU_2792491_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7918" checksum="20E68B3F6CB382B1" modified="2004-10-11" version="1">MRNSDMDELARRRAAREREAGGDDDTVRFTWTDTLAMIIAAYQVLMPIVLLMIGILLVVYLLFRVFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R70</accession>
    <name>Q67R70_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Pyruvate kinase</fullName>
            <ecNumber evidence="6 15">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">STH838</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 15"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH838"/>
    <dbReference type="EnsemblBacteria" id="BAD39823">
        <property type="protein sequence ID" value="BAD39823"/>
        <property type="gene ID" value="STH838"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH838"/>
    <dbReference type="eggNOG" id="COG0469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_9"/>
    <dbReference type="OMA" id="QVPIVQK"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
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        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 15 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 19" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="16">
        <location>
            <begin position="3"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="18">
        <location>
            <begin position="357"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="17">
        <location>
            <begin position="503"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAD39823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="584" mass="62459" checksum="A3FE0C11427EA0CB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD0</accession>
    <name>Q67RD0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spore coat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH778"/>
    <dbReference type="EnsemblBacteria" id="BAD39763">
        <property type="protein sequence ID" value="BAD39763"/>
        <property type="gene ID" value="STH778"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH778"/>
    <dbReference type="eggNOG" id="COG2334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042636_0_0_9"/>
    <dbReference type="OMA" id="QMALVWK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014255">
        <property type="entry name" value="Spore_coat_CotS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02906">
        <property type="entry name" value="spore_CotS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0946">Virion</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="435" mass="48944" checksum="F5AE9680A4B458DE" modified="2004-10-11" version="1">MNNSPVIIYVPAGAGIYVPPTGGVQIIGSGRAAPGDEPLLTARLILDPEDDAPPGPVPEPRWDAKADRRGAQVSAVAPARRNQEEALPDLTVPPEIQDLAERAAKAWDMKVGSWTVAATKPEKGWGAIWRIETNRGPRALKLLHRPFERNLFSIGAQDYLVRRKARVAPLVPTRDGKLYTVVDGRMFIVAEWIQGLRQAPKDTVDGAALLCSGLAEFHRRSLGYRPPSGAAPSSRLHRWPRVYRKLRTKLDWFEHLARAYREMPASPLLLEVLPRFKAQADEAIRMLEASAYRKLAARGEEAWGLAHQDYGWSNGQVGPDGKVWIIDLDGVAFDLAFRDLRKLITGTMDDRGDWDLTWMKAMIRAYHEVHPIEPEAMQVMLIDMYLPNEFYKLVKDVLYDPNLLDATMVQALQRLLITDEHKQQALRELGLRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ7</accession>
    <name>Q67RJ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anaerobic dimethyl sulfoxide reductase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dmsC2</name>
        <name evidence="2" type="ordered locus">STH711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194844.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH711"/>
    <dbReference type="EnsemblBacteria" id="BAD39696">
        <property type="protein sequence ID" value="BAD39696"/>
        <property type="gene ID" value="STH711"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH711"/>
    <dbReference type="eggNOG" id="COG3302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064909_1_1_9"/>
    <dbReference type="OMA" id="MVRVYNT"/>
    <dbReference type="OrthoDB" id="909963at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019645">
        <property type="term" value="P:anaerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007059">
        <property type="entry name" value="DmsC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38095">
        <property type="entry name" value="PTHR38095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04976">
        <property type="entry name" value="DmsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="29592" checksum="5391C2DA3326D751" modified="2004-10-11" version="1">METKEFALVLFTVLSQASVGAFILLSWLRLRNRSEAMDAVYRRSIAVLLPIMAVALVASLFHLGRPMLAFTSLRRLATSWLSREIFFSGAFFALLAATVLLEKSAPGRKALSALTALAGAVAVFSMGAVYTATVIPAWQGWGTYAAFVGTTVLAGAGLAAGLVAFFGRSVEEAAGDLQQLVGAAIAALILGLVAYPLYLASLGNGGAEAQASLQLLGGPFAGWLALRWALTLAGGAVPLLIAWRRLSAGQKTSGLVYAALVCMVAGELVGRYLFYATGVPMSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL5</accession>
    <name>Q67RL5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH693</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194826.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH693"/>
    <dbReference type="EnsemblBacteria" id="BAD39678">
        <property type="protein sequence ID" value="BAD39678"/>
        <property type="gene ID" value="STH693"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH693"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="200"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="300"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="614" mass="64881" checksum="F5673AD220D96ACC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S03</accession>
    <name>Q67S03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194689.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH555"/>
    <dbReference type="EnsemblBacteria" id="BAD39540">
        <property type="protein sequence ID" value="BAD39540"/>
        <property type="gene ID" value="STH555"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH555"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805940_0_0_9"/>
    <dbReference type="OMA" id="LICESTT"/>
    <dbReference type="OrthoDB" id="1400958at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269960" evidence="1">
        <location>
            <begin position="24"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="35374" checksum="816E5C44143AA7A0" modified="2004-10-11" version="1">MITRWRRSLAAARANLGRSVAALAPGADATDPGNEVLRLGIKDTPAIGDELRRRLAAEPGLAWLTVDRAAPGGRAVDTGLLNPLTGRPMTGSTSGGPVNILLGIIDLCVGTDGGGSVLGPAAATGLAGIIGSGLGLTCPVERVSTDGFAFRPALGVIARDWPTARTGFRALLRASGAAEPPAASLAGLRVALPRPGSVTLPDGADMAAELEPYLRPLWAAGAEPVAVDMTGIAARERAMALLREIWATADLAVTLEGPVDLFGLGDSVVGSLGPPGAALQARGGKYLLRALNLVGCTGIALPVPRLSSGLILAGPPGPVGAARALAAADLLAPSLRMPPLFERYFFQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S24</accession>
    <name>Q67S24_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">era</name>
        <name evidence="13" type="ordered locus">STH534</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9 10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194668.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH534"/>
    <dbReference type="EnsemblBacteria" id="BAD39519">
        <property type="protein sequence ID" value="BAD39519"/>
        <property type="gene ID" value="STH534"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH534"/>
    <dbReference type="eggNOG" id="COG1159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038009_1_0_9"/>
    <dbReference type="OMA" id="WAEVDVI"/>
    <dbReference type="OrthoDB" id="984717at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Era-type G" evidence="12">
        <location>
            <begin position="7"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="11">
        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="9">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="9">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="9">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="9">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="9">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="62"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="35500" checksum="30E157E38E4D7D0B" modified="2004-10-11" version="1">MNGAPFRSGFCSIVGRPNVGKSTLLNAFVAAKLAIMSDKPQTTRNRILGVYNRPDAQVVFLDTPGIHKPRHRLGEYMNKVAIGTIPEVDVVLFVVDGSVPEPGEGDRYVADVVARSGRPAILVVNKMDRVKRGDWYAVMDAYKALAQPEARSENPDPGAPTIEWIDIVPVSALEHKNIDALLDLIVQQMEEGPKYYPEDMITDQPERFVIAEFIREKILQLTRDEVPHSVAVEVEELQRRPTGTVYVSASIYVERESQKGIIIGKRGAMLKEIGARARQDIEQMLGSKIYLELFVKVKEDWRNKESVLRSLGYREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S30</accession>
    <name>Q67S30_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194662.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH528"/>
    <dbReference type="EnsemblBacteria" id="BAD39513">
        <property type="protein sequence ID" value="BAD39513"/>
        <property type="gene ID" value="STH528"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH528"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050521_1_0_9"/>
    <dbReference type="OMA" id="VRARVWY"/>
    <dbReference type="OrthoDB" id="1194127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010690">
        <property type="entry name" value="YqfD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06898">
        <property type="entry name" value="YqfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02876">
        <property type="entry name" value="spore_yqfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="46561" checksum="86741D3C5C5FF894" modified="2004-10-11" version="1">MLRRLVRYLLGTLRIEVTGGDIERFLNGCLEEGILLWDLRRTPERLHATMMLADFFQLRPVARAGRCRVRIRARHGLPFLTRRLRRRPFLLAGALGGLAALVWAGSHLWVVDVRINGPGYLDPRAILAVAAEAGLRQGAWKARIDLDHVTQHLKSRVEELSWAVVRVDGTRAVIEVVEKGAVTPPDQAQCVHLVARKAGVVEQVIPLQGEPLVKKGDVVQPGEMLVECALRYWAGGRPVVIPGTPPPPRTDLARTVVAQAAVKARVAYRQYYEYPAVEQVKEPTGRRHVQWVLNWNGRSIILRGRGPVPFAEYEVTTRTLAPGQWRNWSPPVEIVIREALEVSTRAEPVPVEVLTERARAAMERRLAWILGPNDRVLTPLRAEVVQQDGGYVGILVTVETLEEVSAPLEGPMLTMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG2</accession>
    <name>Q67SG2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">T2SSF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH396</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194530.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH396"/>
    <dbReference type="EnsemblBacteria" id="BAD39381">
        <property type="protein sequence ID" value="BAD39381"/>
        <property type="gene ID" value="STH396"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH396"/>
    <dbReference type="eggNOG" id="COG2064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1019143_0_0_9"/>
    <dbReference type="OMA" id="HHANKIV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="133"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="28964" checksum="957B7B984A802740" modified="2004-10-11" version="1">MPLVIALFLSGAVFLLLLPRGRQGGGEAPWPGPRWPAVRRLASRLAGILASGRLARELAVSGLELTLEELALRKIRLAGLALACALLLALLGRPGLATLGFAGAVWAFQAPDLEVTRAAAARRRAVQRDLPYFLFTLAVLAESGMQLLPALDHYARNAAAPLGREVRSALAEIRMGQPPGMALLEMAQRLDVRDLTRAVGALVQTMEKGTEGLAAALRAQAAEAWDRRRRLAQEAGAQASVKLLVPLVLFVLPAVLAMAAGPAIYAFLTQLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH8</accession>
    <name>Q67SH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39365">
        <property type="protein sequence ID" value="BAD39365"/>
        <property type="gene ID" value="STH380"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH380"/>
    <dbReference type="HOGENOM" id="CLU_1668509_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="158" mass="17361" checksum="909F3636AC99CAC4" modified="2004-10-11" version="1">MTMRRWLALLLGAGLLCAGVLYAGAAPGEWRSAYQIRRRAAELAQAADVMVLAEVGEQVLPSYPAEGRVYTDTVVSVVRGGGFAAGTELTLRLPGGRTDDLEVIVDYLHPFPDKGGQVFLLLKEAGEKYDVLAFMELVDGRPRWPEGRAYMRYLSQLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-08-21" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI1</accession>
    <name>MINE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division topological specificity factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">minE</name>
        <name type="ordered locus">STH377</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MinE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194511.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SI1"/>
    <dbReference type="STRING" id="292459.STH377"/>
    <dbReference type="EnsemblBacteria" id="BAD39362">
        <property type="protein sequence ID" value="BAD39362"/>
        <property type="gene ID" value="STH377"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH377"/>
    <dbReference type="eggNOG" id="COG0851">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137929_1_1_9"/>
    <dbReference type="OMA" id="VHDRANV"/>
    <dbReference type="OrthoDB" id="2038628at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032955">
        <property type="term" value="P:regulation of division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00262">
        <property type="entry name" value="MinE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005527">
        <property type="entry name" value="MinE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036707">
        <property type="entry name" value="MinE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33404">
        <property type="entry name" value="PTHR33404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03776">
        <property type="entry name" value="MinE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55229">
        <property type="entry name" value="SSF55229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01215">
        <property type="entry name" value="minE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000298194" description="Cell division topological specificity factor">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00262"/>
        </source>
    </evidence>
    <sequence length="92" mass="10562" checksum="FB851D25DD9A168A" modified="2004-10-11" version="1">MLDLISRVFGRDHSSADIARERLRLVLVHDRTNVSPQFLETLKEELIEVISRYMEIEEEGMDVTLQTAENQVALVANIPVRRMKRAAVGSKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ9</accession>
    <name>CLPX_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpX</name>
        <name type="ordered locus">STH359</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194493.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SJ9"/>
    <dbReference type="SMR" id="Q67SJ9"/>
    <dbReference type="STRING" id="292459.STH359"/>
    <dbReference type="EnsemblBacteria" id="BAD39344">
        <property type="protein sequence ID" value="BAD39344"/>
        <property type="gene ID" value="STH359"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH359"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_9"/>
    <dbReference type="OMA" id="HYKRVQA"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="term" value="F:unfolded protein binding"/>
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    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
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        <property type="entry name" value="ClpX_bact"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Znf_CppX_C4"/>
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        <property type="entry name" value="Znf_CppX_C4_sf"/>
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        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000160438" description="ATP-dependent Clp protease ATP-binding subunit ClpX">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="ClpX-type ZB" evidence="2">
        <location>
            <begin position="2"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <sequence length="424" mass="47166" checksum="8ECA5F841B807752" modified="2004-10-11" version="1">MFKFTGEDKGQLKCSFCGKYQDQVKRLVAGPGVYICDECIELCNEIIEEELSEEVDFELKDVPKPAEIKAILDQYVIGQERAKRTLAVAVYNHYKRINLGSKLDDVELQKSNILMLGPTGSGKTLLAQTLARILNVPFAIADATSLTEAGYVGEDVENILLKLIQAADYDIEKAERGIVYIDEVDKIARKSENPSITRDVSGEGVQQALLKILEGTIASVPPQGGRKHPHQEFIQINTTNILFICGGAFDGIDKIIANRVGRSGLGFGADIRSKQEQNVGELLKQIMPEDLLKFGLIPEFIGRLPIVVTLDALDEDALVRILREPKNALVKQYQKLLQLDNIELEFEEEAVRAIAKEALRRNTGARGLRAIIEDIMTDVMYEVPSRTDVTKCVITKDVVLKKQEPILVTTNDAKSRRTKKEESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK6</accession>
    <name>Q67SK6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH352</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194486.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH352"/>
    <dbReference type="EnsemblBacteria" id="BAD39337">
        <property type="protein sequence ID" value="BAD39337"/>
        <property type="gene ID" value="STH352"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH352"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042017_0_0_9"/>
    <dbReference type="OMA" id="PQDIQAP"/>
    <dbReference type="OrthoDB" id="1203651at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023873">
        <property type="entry name" value="FeFe-hyd_GTPase_HydF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041606">
        <property type="entry name" value="HydF_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040644">
        <property type="entry name" value="HydF_tetramer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18128">
        <property type="entry name" value="HydF_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18133">
        <property type="entry name" value="HydF_tetramer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03918">
        <property type="entry name" value="GTP_HydF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="14"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HydF_dimer" evidence="2">
        <location>
            <begin position="182"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="HydF_tetramer" evidence="3">
        <location>
            <begin position="284"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="44386" checksum="8FAE782050A4A4A7" modified="2004-10-11" version="1">MAAERTPTGQRLHIAFFGRRNAGKSSLINALSRQAVSLVSPVPGTTTDPVRRPMELHPLGPVVLIDTAGIDDEGDLGRLRVERSFQVLDQTDLAVLAVPAGEEPGEAEERLVAELKRRGTPAVGAVTKADLDPAGARRQAARLEERFGFSFCACSCIGQPGVAALRQRIIAAAPVDPTEVPLLADLVRPGDLIVLVVPLDSGAPKGRLILPQVQAIREVLDAGGIAVTVRDRELPDALKSLGRRPALVVTDSQVFGTVNRAVPPEIPLTSFSILMARHKGDLAELVRGAQAIDRLRPGDRVLIAETCTHHQQKDDIGTVKIPRWLRERVGGELQFEWAHGGEMPEDLSPYRLIVHCGGCMVNRRLMLSRIRRAQAAGVPIVNYGVCIAHLNGILPRVLTPFREQGGMSGGNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK8</accession>
    <name>Q67SK8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">PTS system N-acetylglucosamine-specific enzyme IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194484.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH350"/>
    <dbReference type="EnsemblBacteria" id="BAD39335">
        <property type="protein sequence ID" value="BAD39335"/>
        <property type="gene ID" value="STH350"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH350"/>
    <dbReference type="eggNOG" id="COG1263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012312_1_0_9"/>
    <dbReference type="OMA" id="HALYPVF"/>
    <dbReference type="OrthoDB" id="2035550at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013013">
        <property type="entry name" value="PTS_EIIC_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51103">
        <property type="entry name" value="PTS_EIIC_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="374" mass="39936" checksum="471A7C7352BA97B5" modified="2004-10-11" version="1">MLAQLQKVGKGLMMPVAVLPAAGLLLRLGQPDVLDWPWMANAGDAVFSNLPLLFAVGLSVSLAKDSGVAGLSGVVAYFVMTNVAVTINAEINMGVLAGLIAGILAANMYGRFKDTKLPDYLGFFGGRRFVPIVTAGAATVLGLIFGFIWPPIQHGIGAVGNWMTAAGAIGVGVFGLLNRLLIPLGLHHVINSIVWFMFGEFQKPDGTVATGDLWRFFAGDPSAGFFMAGWFPIMMFGLIGAAFAMTHAARPENRKRVGGIMLSAAFTSFLTGITEPIEFAFMFVAPLLYAIHAVLAGTSMAIAQLLDIHHGFTFSAGLIDFVLNYGIASDNAWLLIPIGLVYGVVYYVVFRWVIVRFNIPTPGREPEESHVPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL0</accession>
    <name>Q67SL0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PTS system enzyme IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH348</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194482.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH348"/>
    <dbReference type="EnsemblBacteria" id="BAD39333">
        <property type="protein sequence ID" value="BAD39333"/>
        <property type="gene ID" value="STH348"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH348"/>
    <dbReference type="eggNOG" id="COG2190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012312_5_1_9"/>
    <dbReference type="OMA" id="ESEIIHY"/>
    <dbReference type="OrthoDB" id="1481289at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIA type-1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="164" mass="16737" checksum="BFF3338AA18E62A7" modified="2004-10-11" version="1">MTVVTLLAPITGRTVPLDDVPDPVFAGRMLGDGVAIDPSGDLVVAPAAGEVVALFPTGHALALRMDSGVEILLHLGLDSSRAKGVFRPVVALGDRVETGQPLIYMDLEGLRAQSRSPLSPLVVLSAGAGARADVAVEVLASGPVVAGRDVVCRLLEGRKGDATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL1</accession>
    <name>Q67SL1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194481.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH347"/>
    <dbReference type="EnsemblBacteria" id="BAD39332">
        <property type="protein sequence ID" value="BAD39332"/>
        <property type="gene ID" value="STH347"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH347"/>
    <dbReference type="eggNOG" id="COG0826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078477_0_0_9"/>
    <dbReference type="OMA" id="HTYIRMA"/>
    <dbReference type="OrthoDB" id="796890at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="30890" checksum="F19862859172F321" modified="2004-10-11" version="1">MRLLDMPPSGLIQLTRANLLQGIRLSEGRLVVAETIVVAPPLIDGVSNAELAAAFGADILLLNCYDCVDPAILGLPGEPGKGQTCQDLKELVGRPVGVNLEPSDRVPPGRRATPENARRAVEQGADFICLTGNPHTLVTNAGILESLRHIREAVGDRTVLVAGRMHAAGSGLSPGRGLITPEETEAFVDAGADVVLLPAPGTVPGTTVEEVRACVQAAHRKGALAMAAIGTSQEGADEATIRQIALSAKMAGVDLHHLGDAGYHGVAVPENILAYCLAVKGRRHTYRRMALSARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP9</accession>
    <name>Q67SP9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH309</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH309"/>
    <dbReference type="EnsemblBacteria" id="BAD39294">
        <property type="protein sequence ID" value="BAD39294"/>
        <property type="gene ID" value="STH309"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH309"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010123_0_0_9"/>
    <dbReference type="OMA" id="HLSTNMC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031:SF539">
        <property type="entry name" value="PTHR24031:SF539"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18019">
        <property type="entry name" value="HD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="11">
        <location>
            <begin position="12"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="234"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="719" mass="81720" checksum="52920E5CA4D09591" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS6</accession>
    <name>Q67SS6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH282"/>
    <dbReference type="EnsemblBacteria" id="BAD39267">
        <property type="protein sequence ID" value="BAD39267"/>
        <property type="gene ID" value="STH282"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH282"/>
    <dbReference type="eggNOG" id="ENOG5033NCM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_918060_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="34276" checksum="F53663D543BA7101" modified="2004-10-11" version="1">MYTPPMQKSDMEPSLLARLVISPCGRNCGRASPETPICAQRRDGWVPHAELRGKISQSGSNAFDRLEDLLTSDVFGRLRYLPPDRGLIPVLQQAVNLQGNRGCPLPQTVSPEPDYQFWPWLKRCEPDVLIQLSGDEGKPFLVLVECKYRSPKSDRPGAEANGDVEERSALPDQLAREFLDLTDWLSEQGGDGILLYVTGHSVLPRSDLETSAAALAGEAGSNRGNRLRERFLDRTYWIGWPAMWAVFRELYTQEQDPYRRRILGDLLDLLAHKGYRRFRGWPEPNPAGPLPPPPSPVWYRRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV3</accession>
    <name>Q67SV3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH255</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH255"/>
    <dbReference type="EnsemblBacteria" id="BAD39240">
        <property type="protein sequence ID" value="BAD39240"/>
        <property type="gene ID" value="STH255"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH255"/>
    <dbReference type="eggNOG" id="COG1464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067080_0_0_9"/>
    <dbReference type="OMA" id="HIEPMGV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="29991" checksum="BFC5FED69C25A0A6" modified="2004-10-11" version="1">MLAGCGGAKGSPAQGEQPAQQAPAQDQGQVQGPEQTQEPVTLTVGATVTPHSEILEFIKDKAEAEGIKLDIVVFTDYVQPNLALADGSLDANYFQHVPYLEQFSADRGLDLVSVGPVHLEPLGLYALELTSLDQLPDGATVSIPDDPTNAGRALKLLADHGLIELKEGVGVKATPNDIVANPKSLKIEMLQAEFLPRSLQDVDAAVINTNYYLDAAKNLGVKANVLARESAENNPYANIVAVRRGDENRPEIKKLMELLQSEAVARFINENYDGAVVPAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI4</accession>
    <name>Q67TI4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Oxygen sensor histidine kinase NreB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nitrogen regulation protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH24</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxygen, probably at the conserved histidine residue, and transfers its phosphate group probably to a conserved aspartate residue of NreC. NreB/NreC activates the expression of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH24"/>
    <dbReference type="EnsemblBacteria" id="BAD39009">
        <property type="protein sequence ID" value="BAD39009"/>
        <property type="gene ID" value="STH24"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH24"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="37"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="178"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="373"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD39009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="45528" checksum="F53AD089C1AFEB3A" modified="2004-10-11" version="1">MDAVLHGRAAAAVGLQAAGRGKERVHMPASAYGSEFEILEVLPVGIFILDRSLRVAFVNETMENYFGISRSRLIGRYKPALVQEQIRHIIQEGDEFARRLLASYENNTYPEQFRCRVLAGRNRRARWLEHRSKPITTGPYTGGRSELYTDITAQVESEQEREFLHNQIMLIQEREKARLAQDLHDGLGQSVVAVKLMLEELECGMAARGIQDPACHRLRDIISYVERLSHEVRAISFDLTPSMLRPLGLTETIAWMVDHCADLYNLTIDFTSHGLSHRRLPDAVETQLFRIFQEALNNVVKHARASEVQVRLVYTHPTVILTMKDNGQGFNRNTAFEGLGIKSMERRAQQLGGRFKLVSGSGRGTTVRVEVPVEEGGHGEDPGVGGRRSRTGPDRAYQDPERSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J94</accession>
    <name>Q67J94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative membrane efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3275</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197387.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3275"/>
    <dbReference type="EnsemblBacteria" id="BAD42256">
        <property type="protein sequence ID" value="BAD42256"/>
        <property type="gene ID" value="STH3275"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3275"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_1_2_9"/>
    <dbReference type="OMA" id="YANIYSP"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="61"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="102"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="37241" checksum="E358B85CE1AA940A" modified="2004-10-11" version="1">MKRFFPAAWLALGALILSGCGLASGRGGEPTVEAVAVAVAEARTGTLTATSRASGQLSPIRNITITAKVPGKVVAVHKQMGDTVQEGDLLVELDDKDLASQLAAAQAQYTQAQAQRAEAARQASRLEALLEQGAVSQQQAEQIRTQLTLANAQVEAARAQLELATSSYEGARITAPADGVLAARYVEPGGMVGAGSPLFQLVDLSTVVVEAGVAEAAINAVRPGVAVTVSVPALNRTFTGEVESVSPQMDLQTRSYRVRVTLPNPDGVLKGGMFAEVIIPLAEQTGVLVPVNALVETGSEPHVYVVKDGVAHRTPVTVVSRSDEQVLVEGIAEGDQLVVAGQNRLYDGAPVRLGGGSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA0</accession>
    <name>Q67JA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arginine/lysine/ornithine decarboxylases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3269</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197381.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3269"/>
    <dbReference type="EnsemblBacteria" id="BAD42250">
        <property type="protein sequence ID" value="BAD42250"/>
        <property type="gene ID" value="STH3269"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3269"/>
    <dbReference type="eggNOG" id="COG1982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025925_2_1_9"/>
    <dbReference type="OMA" id="WSTLLTE"/>
    <dbReference type="OrthoDB" id="485164at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00615">
        <property type="entry name" value="Orn_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000310">
        <property type="entry name" value="Orn/Lys/Arg_deCO2ase_major_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008286">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036633">
        <property type="entry name" value="Prn/Lys/Arg_de-COase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01276">
        <property type="entry name" value="OKR_DC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03711">
        <property type="entry name" value="OKR_DC_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55904">
        <property type="entry name" value="SSF55904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00703">
        <property type="entry name" value="OKR_DC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OKR_DC_1" evidence="3">
        <location>
            <begin position="221"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="488" mass="52935" checksum="DB025F496D117190" modified="2004-10-11" version="1">MNQHETPLFTGLVRHAERNPIQFHIPGHKKGTGMAPEFRAYIGERALAIDLINIAPLDDLHNPHGIIREAQALAAEAFGADHSFFSVQGTSGAIIAMIMTVCGPGDKILVPRNVHKSVLTGIVLSGALPIFMNPDLDYELGIAHGVSLEAVRHALDQHPDAKGVLVINPTYYGVAADLKGIVELAHSRGVPVLVDEAHGVHLCFHDELPLSAMQAGADMAATSVHKLGGSLTQTSILNVREGFINPGRVQAILSMLTTTSTSYLLLASLDVARKQLATRGRELIDRAVRLAERARREIAEIPGLYCLGPDVVGQSSSRFDLDPTKLCINVKELGVTGVEVEAILREEFNIEVELSDLYNILCIISLGDTAEDVDALVAALGEISRRFYRQRPANVVKVKVPVTPILAMHPRTAFYAETEVVPIREAEGRVIAESIMVYPPGIPILLPGEIVAEENLDYLEECIQAGLPVQGTEDPEIRMVKVVKDAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE8</accession>
    <name>Q67JE8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197333.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3220"/>
    <dbReference type="EnsemblBacteria" id="BAD42202">
        <property type="protein sequence ID" value="BAD42202"/>
        <property type="gene ID" value="STH3220"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3220"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_9"/>
    <dbReference type="OMA" id="LDKYATH"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="136"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="22670" checksum="873C26E1EB94DD19" modified="2004-10-11" version="1">MRILIADDHRVVRTGLRMLLESHPDFEVVGEASSGPETVQKAQELRPELVILDLSMPDGNGLLYLRQLSEMTRVLVLTMHDDPAYVRQVIQAGGSGYVLKEAADVELFSAIRAVLSGQTYIYPTLAAKLVEEKAGGERKSVPLSPRELEVLRLVALGHTNQEIALQLNVSVRTVETYKTRICEKLGVYTRSEMVRYALEHKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH2</accession>
    <name>Q67JH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3196"/>
    <dbReference type="EnsemblBacteria" id="BAD42178">
        <property type="protein sequence ID" value="BAD42178"/>
        <property type="gene ID" value="STH3196"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3196"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052943_0_0_9"/>
    <dbReference type="OMA" id="VYDDKMR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16_C" evidence="1">
        <location>
            <begin position="186"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="427" mass="47740" checksum="7AB14EE3B13C2047" modified="2004-10-11" version="1">MADHFTRFPLHEGVNLYVQPTRKFKTTTVYIYFHMPLEPVTVTYNALLPMVLARASADFPTTAALSRHLDELYGASFSVDVARRGEVQSIVFRLEVAGDHHIPGERGLLLRGLDVLASVITRPLLVGDGFRPDYVEQERNNLRQMIEGLINDKRRYAMVRCTAEMCAGEPFALHRLGRVEDLEGATPQSLLAHHRRVLTEAPVDIFILGDVDPEQVAQEVPRRLPIPAGERRFPDTLVKRRPDGPVRAVVDRMDVNQGVVVIGFRTGITLRDELYFPMLVANGVLGGFSHSKLFQEVREKHSLAYFAYSAIETVKGVGYMYAGVEFADAEKCRAIMLEQLKALQEGALTEAELEMTKATLVNDMLSAADSPGAMAELAVDQVFSGRDLSIDERVTRYRQVTREQVVEAARHFTPDTVYLLTRKEGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ6</accession>
    <name>Q67JJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell division protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ftsW</name>
        <name evidence="7" type="ordered locus">STH3172</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3172"/>
    <dbReference type="EnsemblBacteria" id="BAD42154">
        <property type="protein sequence ID" value="BAD42154"/>
        <property type="gene ID" value="STH3172"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3172"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_2_1_9"/>
    <dbReference type="OMA" id="YQLNLSM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011923">
        <property type="entry name" value="RodA/MrdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474:SF1">
        <property type="entry name" value="PTHR30474:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02210">
        <property type="entry name" value="rodA_shape"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="379" mass="41461" checksum="B0655481C232B17C" modified="2004-10-11" version="1">MFALYWHGRFWRMIDWPLFAIVLAITAIGFVVISSAAPSYEVKADLIKQAAALVMGIFAWAILLLLDYNELRALHWWIYGFNVAMLLAVVAFGVEVMGNKNWLDLGVIMVQPSELGKVLLIITLAKQLDDMERLDAWWHLAPPILHVLPVLGAVVLQKDLGTALVFAVIGVVMVYGRGFPGRKLLAAGILLAAFVVGSVWSHYTYGTVFPLNINPGQWSRIDAFLFPEKDPQGSGWQVLQSKMAIASGDIWGKGYKQGEYQQNGWLPFPHTDFAFAALVEEWGFVGGAVLLGLYALLFLRLAIIAFSANDRYGTLLVVGVAGLFGAHVLENVGMTMGLMPVTGIPLPFVSYGPTALLANMAAIGLVQSVAARREPLPYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM0</accession>
    <name>Q67JM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Menaquinol-cytochrome C reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3148"/>
    <dbReference type="EnsemblBacteria" id="BAD42130">
        <property type="protein sequence ID" value="BAD42130"/>
        <property type="gene ID" value="STH3148"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3148"/>
    <dbReference type="eggNOG" id="COG1290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031114_0_1_9"/>
    <dbReference type="OMA" id="WATQTGM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005797">
        <property type="entry name" value="Cyt_b/b6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00033">
        <property type="entry name" value="Cytochrome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51002">
        <property type="entry name" value="CYTB_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="CYTB_NTER" evidence="2">
        <location>
            <begin position="21"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="29270" checksum="6663C8C1754B652D" modified="2004-10-11" version="1">MGLWARFKNTTLYKSIYRNEYPDTPRNQVLVILNSVVLHLHPVKVPRRAVRMSYTWGLGGLSLLMFLMLTVTGVWLMFYYVPSVPGAYESMKALETDVIFGPFARNLHRWAAHGMVIAVWLHMTRVFYTGSYKPPREFNWVIGVALFLITVLLSFTGYLLPWDQLAYWAIQVGSNMAKVTPVIGTYVQRALLDGYDIGPATLVRWYTLHVILLPLACIALMAVHFWRIRKDGGISTPIPYDEDEVEGAVEAGLAADDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM4</accession>
    <name>Q67JM4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197257.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3144"/>
    <dbReference type="EnsemblBacteria" id="BAD42126">
        <property type="protein sequence ID" value="BAD42126"/>
        <property type="gene ID" value="STH3144"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3144"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_891174_0_0_9"/>
    <dbReference type="OMA" id="FENAMSP"/>
    <dbReference type="OrthoDB" id="1871658at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008168">
        <property type="entry name" value="Cyt_C_IC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00605">
        <property type="entry name" value="CYTCHROMECIC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="42"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="187"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="34916" checksum="827C0A665C04A534" modified="2004-10-11" version="1">MRNERKYKIITLLSLALALLLLGLGVREPFRMATAEYRQHEEYVLKGAQIYAENCVQCHGPYGEGVVGMPLNRAEFRVDPASPAGKEIYAYLFDTIARGREGNAAHYQWVRVKTPEGKDAWMSYTEMPAWLKEHGGPLDEEAVKALTLFIMYDDPSGSQWYMVGDSSKAPIPAADLTPDETGVIPLPDADVPEEVNVTAKALLRDLAKTQCLTCHRIGSKGAYIGPDLSQLGLWGIDKEFLVEWIKRASGPNAMPHDERMPIYWSQNRAITSTEIDLSERVVSEGPYYMPAFEDRLTDEEISVIADYLLGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN0</accession>
    <name>Q67JN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3138"/>
    <dbReference type="EnsemblBacteria" id="BAD42120">
        <property type="protein sequence ID" value="BAD42120"/>
        <property type="gene ID" value="STH3138"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3138"/>
    <dbReference type="HOGENOM" id="CLU_2720848_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="7743" checksum="6CA1C3573F36ABAE" modified="2004-10-11" version="1">MTGAHATGHHLNEAAERGEPARDVPLEGLVWIEKEDQAKQGMARKGDMRDAAAAPDRPHPAGRPDQGGRQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ5</accession>
    <name>Q67JQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stage 0 sporulation protein H, RNA polymerase sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spo0H</name>
        <name evidence="4" type="ordered locus">STH3113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197227.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3113"/>
    <dbReference type="EnsemblBacteria" id="BAD42095">
        <property type="protein sequence ID" value="BAD42095"/>
        <property type="gene ID" value="STH3113"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3113"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090333_0_1_9"/>
    <dbReference type="OMA" id="LEWKVLM"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014218">
        <property type="entry name" value="RNA_pol_sigma-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016371">
        <property type="entry name" value="RNA_pol_sigma-H_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002939">
        <property type="entry name" value="RNA_polymerase_sigma-H_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02859">
        <property type="entry name" value="spore_sigH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="221" mass="25700" checksum="1630DE3D2CA1DEBB" modified="2004-10-11" version="1">MTATPQRNVYVDYDSMMDEDIVEFAREGSTEALEYLINKYKNFVRAKARSYFLIGADRDDITQEGMIGLYKAIRDFRADKLSSFRAFAELCITRQIITAIKTATRQKHIPLNSYVSLNKPIYDDDSDRTLLDVISGSKVTDPEELIISREEFGDIEGKMEEILSDLEWKVLMSYLDGKSYHEIAVELHRHVKSIDNALQRVKRKLERYLENRNQSLERGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS3</accession>
    <name>Q67JS3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3095"/>
    <dbReference type="EnsemblBacteria" id="BAD42077">
        <property type="protein sequence ID" value="BAD42077"/>
        <property type="gene ID" value="STH3095"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3095"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424467_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033463">
        <property type="entry name" value="sCache_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17202">
        <property type="entry name" value="sCache_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="410"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="615"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="645" mass="67815" checksum="4E16417A2EBFA523" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS5</accession>
    <name>RL33_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmG</name>
        <name type="ordered locus">STH3093</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JS5"/>
    <dbReference type="SMR" id="Q67JS5"/>
    <dbReference type="STRING" id="292459.STH3093"/>
    <dbReference type="PRIDE" id="Q67JS5"/>
    <dbReference type="EnsemblBacteria" id="BAD42075">
        <property type="protein sequence ID" value="BAD42075"/>
        <property type="gene ID" value="STH3093"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3093"/>
    <dbReference type="eggNOG" id="COG0267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_0_2_9"/>
    <dbReference type="OMA" id="RYTTMKN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00294">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018264">
        <property type="entry name" value="Ribosomal_L33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43168">
        <property type="entry name" value="PTHR43168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00582">
        <property type="entry name" value="RIBOSOMAL_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000356757" description="50S ribosomal protein L33">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00294"/>
        </source>
    </evidence>
    <sequence length="54" mass="6257" checksum="B51595A723B8E5F7" modified="2004-10-11" version="1">MAKASARVIITMACTDCKNRNYSTYKNKKNDSGRLELRKFCPTCGHHTLHRETR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX2</accession>
    <name>RL17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplQ</name>
        <name type="ordered locus">STH3046</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197161.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JX2"/>
    <dbReference type="SMR" id="Q67JX2"/>
    <dbReference type="STRING" id="292459.STH3046"/>
    <dbReference type="EnsemblBacteria" id="BAD42028">
        <property type="protein sequence ID" value="BAD42028"/>
        <property type="gene ID" value="STH3046"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3046"/>
    <dbReference type="eggNOG" id="COG0203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_2_2_9"/>
    <dbReference type="OMA" id="YAVTELF"/>
    <dbReference type="OrthoDB" id="1838961at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000267952" description="50S ribosomal protein L17">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <sequence length="113" mass="12645" checksum="E186E2E081650F3A" modified="2004-10-11" version="1">MAKYQKLGRDSSARKALFRAVVTALFDKERIETTEAKAKAVQSIAEEMITLAKRGDLHARRQALAYIYDESVVTKLFNQIAPRYADRNGGYTRVIRTGVRKGDAAPMAILELV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-29" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX3</accession>
    <name>ECFA1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Energy-coupling factor transporter ATP-binding protein EcfA1</fullName>
            <shortName evidence="1">ECF transporter A component EcfA1</shortName>
            <ecNumber evidence="1">7.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ecfA1</name>
        <name type="synonym">cbiO1</name>
        <name type="ordered locus">STH3045</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197160.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JX3"/>
    <dbReference type="SMR" id="Q67JX3"/>
    <dbReference type="STRING" id="292459.STH3045"/>
    <dbReference type="EnsemblBacteria" id="BAD42027">
        <property type="protein sequence ID" value="BAD42027"/>
        <property type="gene ID" value="STH3045"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3045"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="YALELCP"/>
    <dbReference type="OrthoDB" id="1713578at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005876">
        <property type="entry name" value="Co_trans_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030947">
        <property type="entry name" value="EcfA_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01166">
        <property type="entry name" value="cbiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04520">
        <property type="entry name" value="ECF_ATPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51246">
        <property type="entry name" value="CBIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000288016" description="Energy-coupling factor transporter ATP-binding protein EcfA1">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="6"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01710"/>
        </source>
    </evidence>
    <sequence length="287" mass="31221" checksum="A1ED4EC44BC8AD4D" modified="2004-10-11" version="1">MTNPYIVAEGVSYAYGREEADGAAPVRLALDGVDLDVRRGEFLAILGMNGSGKSTLARHLNALLLPRAGRVLVDGLDTREEANLWSIRDRVGMVFQNPDNQIVAAVVEEDVAFGPENQGLPSAEIRARVAEALEAVGMSEHRERSPHLLSGGQKQRVAIAGALAMRPACLVLDEPTAMLDPSGRAEVLAVVRRLNRELGMTVVWITHFMDEAVVADRVVVMAEGRVQMVGTPREVFAQADRIRALRLDLPPAVQAAERLRAQGVPLPRTILTLDELVEALCQLYSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K01</accession>
    <name>Q67K01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Long-chain fatty-acid-CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197131.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3016"/>
    <dbReference type="EnsemblBacteria" id="BAD41999">
        <property type="protein sequence ID" value="BAD41999"/>
        <property type="gene ID" value="STH3016"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3016"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_9"/>
    <dbReference type="OMA" id="KQSDMKA"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="35"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="463"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="555" mass="61974" checksum="71FDAA8D1BE9B244" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K26</accession>
    <name>Q67K26_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Flagellar biosynthetic protein FliP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">fliP</name>
        <name evidence="14" type="ordered locus">STH2991</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in the flagellum-specific transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FliP/MopC/SpaP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2991"/>
    <dbReference type="EnsemblBacteria" id="BAD41974">
        <property type="protein sequence ID" value="BAD41974"/>
        <property type="gene ID" value="STH2991"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2991"/>
    <dbReference type="eggNOG" id="COG1338">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042028_0_1_9"/>
    <dbReference type="OMA" id="MPPNQIL"/>
    <dbReference type="OrthoDB" id="1049349at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005837">
        <property type="entry name" value="FliP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005838">
        <property type="entry name" value="T3SS_IM_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587">
        <property type="entry name" value="PTHR30587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587:SF0">
        <property type="entry name" value="PTHR30587:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00813">
        <property type="entry name" value="FliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00951">
        <property type="entry name" value="FLGBIOSNFLIP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01302">
        <property type="entry name" value="TYPE3IMPPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01103">
        <property type="entry name" value="fliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01061">
        <property type="entry name" value="FLIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7 12" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 12" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="14" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269511" description="Flagellar biosynthetic protein FliP" evidence="13">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD41974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27504" checksum="C2A886BC58EDB1A7" modified="2004-10-11" version="1">MRSARILRPLLLTAALLALAAPAALAQPLEIPTFDFIRPAETPQEGLQTMQLLLLLTVLTLAPAIIILCTSFTRIIVVFGFIRTALGLQQTPPNQVLIGLALFLTAAVMMPTFSEINQTAVQPYLAGEIDQVEAIERGALPLKAFMLRQTREADLGLMLEVSGQPAPEGPEDVSFLTVVPAFALSELKTAFTMGFLIYLPFIIIDLITASLMMSLGMMMVPPTLISLPFKVLLFVLVDGWHLVVESLVRSFTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF8</accession>
    <name>PURL_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="ordered locus">STH2855</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196974.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KF8"/>
    <dbReference type="SMR" id="Q67KF8"/>
    <dbReference type="STRING" id="292459.STH2855"/>
    <dbReference type="PRIDE" id="Q67KF8"/>
    <dbReference type="EnsemblBacteria" id="BAD41840">
        <property type="protein sequence ID" value="BAD41840"/>
        <property type="gene ID" value="STH2855"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2855"/>
    <dbReference type="eggNOG" id="COG0046">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_9"/>
    <dbReference type="OMA" id="FIEPYQG"/>
    <dbReference type="OrthoDB" id="26038at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100495" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="309"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="778" mass="82896" checksum="BEC34D07094EADC4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN8</accession>
    <name>Q67KN8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH-quinone oxidoreductase</fullName>
            <ecNumber evidence="2">7.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nuoC2</name>
        <name evidence="4" type="ordered locus">STH2775</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196894.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2775"/>
    <dbReference type="EnsemblBacteria" id="BAD41760">
        <property type="protein sequence ID" value="BAD41760"/>
        <property type="gene ID" value="STH2775"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2775"/>
    <dbReference type="eggNOG" id="COG0852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_6_0_9"/>
    <dbReference type="OMA" id="THINGDT"/>
    <dbReference type="OrthoDB" id="1735902at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="SSF143243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0874">Quinone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Complex1_30kDa" evidence="3">
        <location>
            <begin position="24"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="156" mass="18216" checksum="42A65EE7255AFC15" modified="2004-10-11" version="1">MDFLKERFPDAQFETHINGDTEILVPADRYEEVVRACYEAGFTFCSVVTAIDWQDRLTLVTSLARINPDEVGVRNIHIKVDLPRQGAHIPSIANLYPGANWHERETYDMYGIRFDGHPNHRRILLPDNWQGGHPLLKDFADRRPKRPRLVRHRFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV6</accession>
    <name>Q67KV6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2705"/>
    <dbReference type="EnsemblBacteria" id="BAD41690">
        <property type="protein sequence ID" value="BAD41690"/>
        <property type="gene ID" value="STH2705"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2705"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_2_2_9"/>
    <dbReference type="OMA" id="WALMREE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="376" mass="41489" checksum="8911CB99E7E9235C" modified="2004-10-11" version="1">MSLYRGKTKKRQMLEVDKQPWLPQQVQVTLHALMGAAKEGLLALAVAVGLDVLRSMMEAEVTAIVGPKGKHNPNRKAFRHGAEEGRVVLGGRKVPIQRPRVRTKDGQEVRLSSYEAFQDEQLLTQAALERMLHGLSTRRYHHGLEPVGDDLPAVATSKSSVSRHFVAATRKALAELLARPLGDQRYLVLMLDGIEVADHTVVVALGITDDGQKQILGLWEGATENATVCRALLTDLVERGLRVDGGILVVIDGAKALRAAVRDVLGARATVQRCQVHKKRNVLDHLPDEARAWVSRKLEQAWRETDYEKARTALTSLAKTLDDKYPGAAASLREGLEETLTVLRLELPEALRKTLRSTNPIESAFEKVRMARSLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ9</accession>
    <name>Q67KZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative poly-gamma-glutamate biosynthesis enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2662</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CapA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41647">
        <property type="protein sequence ID" value="BAD41647"/>
        <property type="gene ID" value="STH2662"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2662"/>
    <dbReference type="eggNOG" id="COG2843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038823_2_3_9"/>
    <dbReference type="OMA" id="YLCTENE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07381">
        <property type="entry name" value="MPP_CapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019079">
        <property type="entry name" value="Capsule_synth_CapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09587">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00854">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGA_cap" evidence="4">
        <location>
            <begin position="88"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="388" mass="40511" checksum="9C9372D0F255ADBE" modified="2004-10-11" version="1">MGCTALSAGPSDAEPGPGAPPAGGPGAGPQNGGTDPGLQNGGAQDGGQGAGNGAGDHPGSGSAGGPDDASGSSPGSGAGTGGGDGTVTITFVGDIMLDRLPGDYLARGEDPFAGVADALTGADLTVGNLECVVSTVGEPVPKWFNFRCHPRVVPVLARYFDAVSVANNHSGDYGKEAFAEQLRLLTEGGVPYFGGGMNDEEAHRPLILERNGLRIALLGYNNVELRSYAAGPDTPGLAWIELDRLAADVRAARAEADIVVVYPHWGYDYMFWHAEDQAEIARVAIDNGADLVVGNHPHVTQPVEIYKDRLIAYSLGNFVFDDFVDVGPDLDEPSRTSWVLTVTIGKDGIRRWETRVARTDDRGFPQWLEGVASPCSDGAPEDGYMCYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L65</accession>
    <name>Q67L65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0749 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2596"/>
    <dbReference type="DNASU" id="2981847"/>
    <dbReference type="EnsemblBacteria" id="BAD41581">
        <property type="protein sequence ID" value="BAD41581"/>
        <property type="gene ID" value="STH2596"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2596"/>
    <dbReference type="eggNOG" id="COG3879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040273_4_0_9"/>
    <dbReference type="OMA" id="GLWPPRV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010273">
        <property type="entry name" value="DUF881"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37313">
        <property type="entry name" value="PTHR37313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05949">
        <property type="entry name" value="DUF881"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="24808" checksum="46A2B212ADF0BB31" modified="2004-10-11" version="1">MNKTYWGLAVVMLFLGLLLTTQFRITSEQHIDPTRLRMDELVVALTDRETELAAAEGRIRELEREVSELRRSLNSPSQEAELERLRLLAGTTAVTGPGVVVTLSETPEAVTAKNRVADEDIWRVVNELYTAGAEAIAVGGVRLTSVSGIRNVGERILVGETMIASPVEIHAIGDPAVLDASLKLRGGVIDLLARWGIKVTVVKSDSLTLPPLKTQPAFRYAKPAESAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L75</accession>
    <name>Q67L75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2586"/>
    <dbReference type="EnsemblBacteria" id="BAD41571">
        <property type="protein sequence ID" value="BAD41571"/>
        <property type="gene ID" value="STH2586"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2586"/>
    <dbReference type="eggNOG" id="COG3039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_2_1_9"/>
    <dbReference type="OMA" id="KIGERND"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13751">
        <property type="entry name" value="DDE_Tnp_1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF772" evidence="1">
        <location>
            <begin position="55"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="356"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="487" mass="55867" checksum="0C457093F5FA5A53" modified="2004-10-11" version="1">MVNAVLREHGHAQRKYELVLIEDLVPQDHLLRKIQAILDTDFIRERTARFYSDRGRPAIDPVVLVKMELIAYLFGIRSDRRLVEEIRVNVAYRWFLGLGLTDPVPHFTTPGKNYSRRWKDSGLFEELFDHVVKQAIDAGYIDGRMIFTDSSHLKANANKRRIAKEGTKGVTLEDIARARERHLAARRAEREECAANDDTEDGLLAAVNADREAHGLKPLPERKEDPQPDLSVDEMTVSLTDPEAAMLRREGKPDGFHYLQHRTVDGRHGFILDVLVTSAAMTDAQVYPTCLSRVDRHGLKVEKVGVDAGYNTLEVLHLLSKRGIQAAVAHRRHPSPKELMGKWRFKYDASRDAYRCPAKQWLTYVTTNRDGYRVYRSDASVCASCPLLGQCTRSTTKQKVIHRHLYEHLREEAREFVKTDEGQRLAQRRRETVERSFADAKELHGLRYARYRGRKRVQHQCLVSALAQNLKKLALLESRRSSYALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM2</accession>
    <name>Q67LM2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2439</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196562.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2439"/>
    <dbReference type="EnsemblBacteria" id="BAD41424">
        <property type="protein sequence ID" value="BAD41424"/>
        <property type="gene ID" value="STH2439"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2439"/>
    <dbReference type="eggNOG" id="COG0608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009736_3_3_9"/>
    <dbReference type="OMA" id="HFMIFKP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="7">
        <location>
            <begin position="85"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="8">
        <location>
            <begin position="319"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="9">
        <location>
            <begin position="455"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="853" mass="92046" checksum="78674AB22788B7F6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ6</accession>
    <name>Q67LQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2405"/>
    <dbReference type="EnsemblBacteria" id="BAD41390">
        <property type="protein sequence ID" value="BAD41390"/>
        <property type="gene ID" value="STH2405"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2405"/>
    <dbReference type="HOGENOM" id="CLU_2425878_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="9919" checksum="B7B166A1E5BF79D2" modified="2004-10-11" version="1">MGYADHRAAFREGRRPFSIARRRRGIGRVRALPVALACGSAHLPSTSDQQARDASSKAPLEQRVPPVGMPDRMAPSLRLTRAAFTAYFAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT4</accession>
    <name>Q67LT4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2377"/>
    <dbReference type="EnsemblBacteria" id="BAD41362">
        <property type="protein sequence ID" value="BAD41362"/>
        <property type="gene ID" value="STH2377"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2377"/>
    <dbReference type="HOGENOM" id="CLU_874145_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="33287" checksum="71CF42D3A19C0F4A" modified="2004-10-11" version="1">MRSWLAPSAPPVQHAPPDAGEHQCGSRDHQAAGSHHPARGGQLGDGRPRGFSGSLRRGRRFGLAGRLRQRGAYNRSGWLLCRRKLRGDGFHGFCGLLRLPGLRLHWLRIAGRGGAGRPAARWVGVTGILRPAGVPRLCGIPRITGIAGITRISRVAGVAGLFRGARILRLARFLRCAGILRFAGLTGRPGLPGLPRPTQLAHLLRDTRFHGIIRVAGILGRARVLRIARLPGVAGVPGVARVPGPAPTTAAGRVHLSGLTGIHRVIRTTWGTGVAGVPGWAGLPGASGPPGFRPVTVGSPPVTPASPLAGPCVRRQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU9</accession>
    <name>Q67LU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cystathionine beta-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196485.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2362"/>
    <dbReference type="EnsemblBacteria" id="BAD41347">
        <property type="protein sequence ID" value="BAD41347"/>
        <property type="gene ID" value="STH2362"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2362"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2669746_0_0_9"/>
    <dbReference type="OMA" id="KWEYSRM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="7955" checksum="09C13C9F19537C93" modified="2004-10-11" version="1">MEFGTRVLHTGREVDSVTGAAAVPIYQVSTFRQPGLNRSGPYDYARSGNPTRQALEAIVADLEQALGYAAAQSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW4</accession>
    <name>Q67LW4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196470.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2347"/>
    <dbReference type="EnsemblBacteria" id="BAD41332">
        <property type="protein sequence ID" value="BAD41332"/>
        <property type="gene ID" value="STH2347"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2347"/>
    <dbReference type="eggNOG" id="ENOG5032RXZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153284_0_0_9"/>
    <dbReference type="OMA" id="IFGAQQY"/>
    <dbReference type="OrthoDB" id="1891852at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025496">
        <property type="entry name" value="DUF4387"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14330">
        <property type="entry name" value="DUF4387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="102" mass="11491" checksum="806B847A3F8404C0" modified="2004-10-11" version="1">MRLRDLASTIRSKNAGVDLITFDILFKTREAYERAKACPALSREGIARLFRIPGDRIYSYVTFDPALAIKFTLRRQRPSGSAGEHDLFGAQQYAPLLDVEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY2</accession>
    <name>Q67LY2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2329</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196452.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2329"/>
    <dbReference type="EnsemblBacteria" id="BAD41314">
        <property type="protein sequence ID" value="BAD41314"/>
        <property type="gene ID" value="STH2329"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2329"/>
    <dbReference type="eggNOG" id="COG1592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095256_0_1_9"/>
    <dbReference type="OMA" id="PQAYFEV"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="5">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="4">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="196" mass="22039" checksum="03B813E28EC6E0E7" modified="2004-10-11" version="1">MNLKGTRTLENLLKAFAGESQAQARYMMYAKVARDEGLEQIAAIFEETALNEAEHGKRFFSLAAAGLEGEMPADVEIQAAYPIAIGNTVENLKAAAAGEHHEWAEMYPEFADIAEAEGFKEIATAFRMIAKAETAHETRYRKLVENLEKGVVFQRDGKYFWKCRNCGYIHEGTAAPKFCPACQYPQAYFEIFVENY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M09</accession>
    <name>Q67M09_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2302"/>
    <dbReference type="EnsemblBacteria" id="BAD41287">
        <property type="protein sequence ID" value="BAD41287"/>
        <property type="gene ID" value="STH2302"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2302"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105788_4_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="18866" checksum="2A94B9DF06CE7B20" modified="2004-10-11" version="1">MQDKPVLIVTRTGRLVTIRPCTPADAPLIAAMYQRLSPRSMRFRYCSGLHIHEEEEAARLCGGDPDAGVVLLALSEGEVVAVGELGQVDEECAEIALLVRDDCQGEGIGTALGQELVEAARERGFTRLVAYMLAENQAMRRIIRGLPFRRTWDSGCGELCVTVELDRPAVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M13</accession>
    <name>Q67M13_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2297</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196420.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2297"/>
    <dbReference type="EnsemblBacteria" id="BAD41282">
        <property type="protein sequence ID" value="BAD41282"/>
        <property type="gene ID" value="STH2297"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2297"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037802_0_1_9"/>
    <dbReference type="OMA" id="VGHMSVK"/>
    <dbReference type="OrthoDB" id="1518604at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="19"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="19652" checksum="690C10D8ED2F48F6" modified="2004-10-11" version="1">MELILGLLIGTAFGFILRRSRVASNACIRGALALTDFHMLKLLLTAVGVTLILVFPLSALGIVNFSVKPTYVVGIVVGGLLFGVGMAVAGFCPGTMLAALPGGDRRFWWAVAGGLAGSLAYALVYEPLEPLLIRPLNLGRLTLPDLLGLNPVVAGVGVGIVILVGVILLDRTTARNKPAIPDLARTGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M56</accession>
    <name>Q67M56_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196378.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2254"/>
    <dbReference type="EnsemblBacteria" id="BAD41239">
        <property type="protein sequence ID" value="BAD41239"/>
        <property type="gene ID" value="STH2254"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2254"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1502741_0_0_9"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="19393" checksum="DEA686B527433EE1" modified="2004-10-11" version="1">MFQRILALAQKEFIQIRRDRRNLAMMLVLPILWLILFGYAFTFDVGEVPVAVVDRSGTTIGAAVADALRSYDRFVPADLPEPSEAGIREAIFRGEVVMGVLIPPGYGDEAHAELHILLDGANLFAAQTAARLIPAALEPAQEVVRAELQARTRAHVAELIAEAAEARKAEVLEGAASPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M66</accession>
    <name>Q67M66_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2244</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2244"/>
    <dbReference type="EnsemblBacteria" id="BAD41229">
        <property type="protein sequence ID" value="BAD41229"/>
        <property type="gene ID" value="STH2244"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2244"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_8_2_9"/>
    <dbReference type="OMA" id="MRVIHAK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="33341" checksum="408D550ADE09B494" modified="2004-10-11" version="1">MRVIHAKLELVQALGMTGVSKSQVSRLCAELDEVVQAFRNRPLEGRYPYVWLDAKYVKVRENGRVSSMALVVAVGVREDGDREILGLDVGPSEDGAFWTAFLRQLVARGLKGVLLAISDSHVGLQEAIRTVLSGASWQRCRVHFMRNLLSYVPKHWQSMVAAAVRTIFAQPDQQAARRQLAVVADNLRPQFPRAAQLLEEAEDDILAYMAFPTEHWRQLHSTNPLERLNREIGRRTDVVGIFPNREAVIRLAGAVLIEQQDEWTAAPRRYFSQASMAKLYTSNQSVSGTDFLTLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M75</accession>
    <name>Q67M75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron-containing alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2233"/>
    <dbReference type="EnsemblBacteria" id="BAD41218">
        <property type="protein sequence ID" value="BAD41218"/>
        <property type="gene ID" value="STH2233"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2233"/>
    <dbReference type="eggNOG" id="COG1454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_0_9"/>
    <dbReference type="OMA" id="GDCLATR"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00060">
        <property type="entry name" value="ADH_IRON_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="3">
        <location>
            <begin position="20"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="33424" checksum="C93A6170997CEA7F" modified="2004-10-11" version="1">MRRLRRRDGRAQRGVAGPGQQVKAHQADLVVAVGGGSAIDVAKATAVLAANGGTAYDYFGVDRVPRPGLPVIAMPTTAGTGAEATPNAIFTNTRTQVKEGIVSPYMMPAVAIVDPELTLTAPPGVTAATGMDALTHAVESFTSVKAAPTTDLFAAEAIRRIGRSIRTAVFRGGDLAARTDMALGSLYAGIAICNAGTGAVHAMAYPLGGQFRVPHGIANASLLPHVLPWNLQGSVEKFAQVARLLGEPVDGLSALAAAERAVAAIQRLCADLGIPSRLSAFGVREEHIPAMAAAAHATRRLMDNNPRNLTVEEVEAIYRSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M96</accession>
    <name>Q67M96_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2212</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2212"/>
    <dbReference type="EnsemblBacteria" id="BAD41197">
        <property type="protein sequence ID" value="BAD41197"/>
        <property type="gene ID" value="STH2212"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2212"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_510820_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269902" evidence="2">
        <location>
            <begin position="27"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="52"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="533" mass="55363" checksum="759A0D6BEBA2A0FB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC6</accession>
    <name>Q67MC6_SYMTH</name>
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        <recommendedName>
            <fullName evidence="2 6">Pyruvate carboxylase</fullName>
            <ecNumber evidence="2 6">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">STH2182</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
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                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + hydrogencarbonate + pyruvate = ADP + H(+) + oxaloacetate + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2182"/>
    <dbReference type="EnsemblBacteria" id="BAD41167">
        <property type="protein sequence ID" value="BAD41167"/>
        <property type="gene ID" value="STH2182"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2182"/>
    <dbReference type="eggNOG" id="COG1038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_0_1_9"/>
    <dbReference type="OMA" id="YAIQSRV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
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    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
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        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
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        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0092">Biotin</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="13">
        <location>
            <begin position="6"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="126"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="14">
        <location>
            <begin position="534"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="1072"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="615"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="9">
        <location>
            <position position="712"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="741"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="743"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="876"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD41167.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1148" mass="125810" checksum="97CB21A282205F8B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD7</accession>
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            <fullName evidence="1">DUF262 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        </lineage>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
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                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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        <property type="gene ID" value="STH2171"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR004919">
        <property type="entry name" value="DUF262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="DUF262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF262" evidence="1">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="39857" checksum="1269E2F21919F610" modified="2004-10-11" version="1">MSVGELVSMYRDGELDIHPEFQRFFRWTDAQKTAFIESLLLGIPIPSIFVAETSSGTWDVIDGLQRISTVLQLMGELKDQDGNRVPPLRLRATRYLPSLQGMVWTAEKEGDIELPQVAKLKIKRARFDIKIILNTSDTKSKYELFQRLNTGGSIATDQEVRNCILIMVNREFYHWLNELRNNKDFQACLPLTDRQLSEQYDMELATRFIVLRRIELDGLSKIEEVGSFLTDEIIKIAEDPTFDRDEAKAAFEKTFSLLASTLGEDSFKRFDLEKNRPVGPFLISMFEIVALGIGYHCLTPGYSKSYEQLVENHMKIWENPRFTSGTGSGVRASSRIPLTIPLGRTIYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF2</accession>
    <name>Q67MF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196281.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2156"/>
    <dbReference type="EnsemblBacteria" id="BAD41141">
        <property type="protein sequence ID" value="BAD41141"/>
        <property type="gene ID" value="STH2156"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2156"/>
    <dbReference type="eggNOG" id="COG3275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091606_0_0_9"/>
    <dbReference type="OMA" id="MVANHAP"/>
    <dbReference type="OrthoDB" id="1731620at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="21696" checksum="9CFE18A5F39B2B1A" modified="2004-10-11" version="1">MAAQQKKNVWAFRFTTLSLVLIPVAVGINYVGKLFAAALKLPLWLDSIGTVLAGMLAGPWIGAISGGVNNIIFGITADPVSFWYLITSIVIGLLVGYLAMTGWIRDFPRAVVLGLIVALAAAVVSTPINVILWEGTTGNVWGDALYTVLRNGGTPVWIASFIDELVVDLLDKVATVIISFLIFKALPQRLVGLFAGEGQVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF7</accession>
    <name>Q67MF7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41136">
        <property type="protein sequence ID" value="BAD41136"/>
        <property type="gene ID" value="STH2151"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2151"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1676960_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="16758" checksum="99B70B0C3ADD9A9D" modified="2004-10-11" version="1">MVFNAGGELLLCRSRDGSAWVLPGGTLDPGEDLRTAAVREAAEEAGVAAEVGPLAYVQEFRSARRSEHVVEIVFRAAAPTGHPSGAALAGRAAEPAGPADRPWQGWRIQDPDGPVRECRWFTRAEVAALSEPVYPEELRDALWEDGGAVHLGLIHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI6</accession>
    <name>Q67MI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2122"/>
    <dbReference type="EnsemblBacteria" id="BAD41107">
        <property type="protein sequence ID" value="BAD41107"/>
        <property type="gene ID" value="STH2122"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2122"/>
    <dbReference type="eggNOG" id="COG4477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_346102_0_0_9"/>
    <dbReference type="OMA" id="THIDEKQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="762"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="816" mass="86797" checksum="ACB4C239855950A2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM0</accession>
    <name>Q67MM0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2088</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196213.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2088"/>
    <dbReference type="EnsemblBacteria" id="BAD41073">
        <property type="protein sequence ID" value="BAD41073"/>
        <property type="gene ID" value="STH2088"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2088"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023166_0_0_9"/>
    <dbReference type="OMA" id="EARMIET"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="294"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="474"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="721" mass="76696" checksum="7704D99BE1B24202" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN3</accession>
    <name>Q67MN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2075</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196200.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2075"/>
    <dbReference type="EnsemblBacteria" id="BAD41060">
        <property type="protein sequence ID" value="BAD41060"/>
        <property type="gene ID" value="STH2075"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2075"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="WREDYRA"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="25813" checksum="F35B1BA5BB771A83" modified="2004-10-11" version="1">MTLLIDARNLVKRYRDRIAVSDVSLSVAPGEVVAVIGPNGAGKSTTLEMILRLRSRDGGEVRYGCAEPRREIGVQLQSTPFFPGLTAAENLRVFAAFYGRRLSRAETDELLVRCRLQHAARTEASRLSGGEQKRLAIAVALVHQPRALFLDEPTAALDPRARHEIRTLIRELAQGGTAVVFTSHDMEEVGRLADRVVLIVNGRVWAEGAPQELLERHGAASLDELYLNLTAEEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN7</accession>
    <name>Q67MN7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2071"/>
    <dbReference type="EnsemblBacteria" id="BAD41056">
        <property type="protein sequence ID" value="BAD41056"/>
        <property type="gene ID" value="STH2071"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2071"/>
    <dbReference type="HOGENOM" id="CLU_1703345_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16737" checksum="170661FF5514F1CE" modified="2004-10-11" version="1">MRARRCRSPRDRGRITGVERDQPAACRAGTTASPEVAKMRKAILFLAALAAVLGGVWLYPVGGRIWTATQVSGVALVREGRRAELSNPAALPHAAQDALLAARLDRFRRLAPALRGGPCVPLDLALRKGNVWVNVSLKCTDGLTFDDIDRDAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS3</accession>
    <name>Q67MS3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SufBD_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196161.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2035"/>
    <dbReference type="EnsemblBacteria" id="BAD41020">
        <property type="protein sequence ID" value="BAD41020"/>
        <property type="gene ID" value="STH2035"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2035"/>
    <dbReference type="eggNOG" id="COG0719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026231_3_1_9"/>
    <dbReference type="OMA" id="PNCESHQ"/>
    <dbReference type="OrthoDB" id="422804at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PTHR43575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01981">
        <property type="entry name" value="sufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="57"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="382" mass="41971" checksum="E45BDC7BC7DBC494" modified="2004-10-11" version="1">MPTVTEPRFLEERRKAAQTLTETLSLPAKTDEAWRRTSLEGLDVLQYRPTAARVTLKGSVPEGVVFANLLDAAAEHAELVQRYLGRLFPMDQNLLTAAQAAHLNGGVLLYVPRGVVVEEPLEVVFEAQDGEAQYLHTLVIAEDNAEVTLLERYEGTGDYLSVHVVEVFPLQGARVRYGYLQNHSQDAWAFTFRRGLTGRDATLDWAGGEFGGALVRSEIVNDVSGEGSQSNLKVVFGATGRQHQDIVASQVHNGSYSRSDILGRGVLSGHAHTVFRGNGQINPAAKHAQTFQRQQALVLSETARADSIPALIINEHEVEGAGHAATVGQLDEEQLFYLMARGLTRQEAVRMLVLAFLSPVLEQIPVQELRDEMIRLMGEKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N26</accession>
    <name>Q67N26_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cobQ</name>
        <name evidence="7" type="ordered locus">STH1932</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1932"/>
    <dbReference type="EnsemblBacteria" id="BAD40917">
        <property type="protein sequence ID" value="BAD40917"/>
        <property type="gene ID" value="STH1932"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1932"/>
    <dbReference type="eggNOG" id="COG1492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_0_0_9"/>
    <dbReference type="OMA" id="PSGNGRM"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269559" description="Cobyric acid synthase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="CbiA" evidence="6">
        <location>
            <begin position="6"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="282" mass="30418" checksum="FC0670692660405A" modified="2004-10-11" version="1">MARSLMFLGTASSVGKSTLAAAFCRILRQEGFRVAPFKAQNMSCAATEIEGGLRISVVQAMQAQAAGVAPRVEMNPVMLLPRTELRSEVIVNGRSLGEMAWQQYTADLHTVALQAVREAIARLEAEFEVIVAEGAGSPVEVNLRDRDLANMKTAELLDADVLLVADIDRGGVFASIVGTLELLPPAERARVKGLVINRFRGDASLFEDGVRWLEERTGLPVIGVIPYLADLGLDEEDSAGLTDVSAPAPSAAAREGALDRLADHVRRHARVDQVLELLARQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N47</accession>
    <name>Q67N47_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196038.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1911"/>
    <dbReference type="EnsemblBacteria" id="BAD40896">
        <property type="protein sequence ID" value="BAD40896"/>
        <property type="gene ID" value="STH1911"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1911"/>
    <dbReference type="eggNOG" id="ENOG5033JQD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523653_0_0_9"/>
    <dbReference type="OrthoDB" id="535127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269457" evidence="3">
        <location>
            <begin position="26"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="486"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="520" mass="57154" checksum="E0E347F3AD25E915" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N75</accession>
    <name>Q67N75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LytR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1883"/>
    <dbReference type="EnsemblBacteria" id="BAD40868">
        <property type="protein sequence ID" value="BAD40868"/>
        <property type="gene ID" value="STH1883"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1883"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_5_4_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269909" evidence="3">
        <location>
            <begin position="45"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="4">
        <location>
            <begin position="87"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="487" mass="52507" checksum="B50927F9E5F9C2D8" modified="2004-10-11" version="1">MRSTSKKSTRKRWHWVIPLVLLLALAAAVRAVALHRPFDSPATADPGRSNPTNPDGSAEEQPPLHTLPELQTFLVMGVDSMEGEWGRSDSLMVVSYNPREQRVAMLSIPRDLWTYIPGRGYDKINHAYAFGGPTLSVETVERLLGMDIDHWVSISFDGFVEVIDALGGVEVNPPEPLYYVDPADRRFGPEGLVIDIQAGPQVMDGLTALKYARFRSDSQGDVGRMRRQQEIIQAAMKKAASPALFARAGQLIPALYHTIGTDMTVGEMLKLATTGRQALTKSPVTGTIEADEYWIDGVFYFGADLVKLRSTAYELLVGEPPGEDFLARAQADNQEYQSVLQREVARSQAAAAARAEEEAANSDGESGETVADPTGDGLGGDAAEGPDGTADETAGAWASVYVVDATGQGAAWDYVAGLEAAGMRVRVHESQAVLSVTMALVRDPELDEESVRQRLESVIPGVVIRMAPDPTAADDIDLVLGTNVLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N76</accession>
    <name>Q67N76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fur family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196009.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1882"/>
    <dbReference type="EnsemblBacteria" id="BAD40867">
        <property type="protein sequence ID" value="BAD40867"/>
        <property type="gene ID" value="STH1882"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1882"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_3_3_9"/>
    <dbReference type="OMA" id="FEIYGTC"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="16538" checksum="627DEFEF3E902939" modified="2004-10-11" version="1">MQVEQVLQRLKDMGCKLTPQRRLIVEVLLSAGEGALTADEIYQQVRQTYPEIGLDTIYRNLRLMARLNVVSEVKLTGRTAQYSLNRELHNHSLVCLECGAEIPLAHCPIKELEAVALEQHGFIISAHRIELFGYCPACAPGADNKIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N83</accession>
    <name>Q67N83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1875"/>
    <dbReference type="EnsemblBacteria" id="BAD40860">
        <property type="protein sequence ID" value="BAD40860"/>
        <property type="gene ID" value="STH1875"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1875"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_42_1_9"/>
    <dbReference type="OMA" id="NTLVAYM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="28790" checksum="92939622F7903588" modified="2004-10-11" version="1">MNGMGTPAREVVVITGAGGMGVAIARRLGPGRRVLLADYSETVLQQAVKQLEGEGFDVHAQQTDVSDPAAVKSLADTAETLGTFRILVHTAGVSPVQAPPERVIAVDVVGTALVLDEFLRLAGPQSVAVCIASMAGAMATLPPEVERALAATPTDQLASLPVLDPQKLDRGAAYSIAKRANQLRVQWASLAWGRKGARVVSVSPGIISTPMGQEELSGPSGDMIRAMIAASGTGRIGTPDDIAAAVEFLVSPAASFITGTDLLVDGGAIAGMRYALSRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N90</accession>
    <name>Q67N90_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1868</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195995.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1868"/>
    <dbReference type="EnsemblBacteria" id="BAD40853">
        <property type="protein sequence ID" value="BAD40853"/>
        <property type="gene ID" value="STH1868"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1868"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692468_0_0_9"/>
    <dbReference type="OrthoDB" id="2053476at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="224"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="398" mass="43543" checksum="B7EF7178825B710C" modified="2004-10-11" version="1">MNYSAYVESVGSQLADRGFEVQAVPPELQGRLALLVSRQEEWGRLLLALAAELNPSDPQERESLAQACGAWVREARGDEVWHMILLFPFDRTVGEEEAEAIAALEERDPGERWGVIPWSADLAVGLLDQHTGFPPVDEAVARILTDVPETPERRFVEVRPRYEARRPWPGLDRFPATRLILALTVAYYLWTVWMGGGLLNLISGPDTLSLVIWGANYSLLTLAERTQQWRLVSHLFLHGGAMHLAFNMWAFWQVGRYCELIYGSARMLFIYFVAGVIGGVASVAVRPGLVISVGASGAILGLMGALIYFATTVRNRRVDWQGLLAPVAINLLFGFVYGRVDNYAHIGGLLGGLLAAFVAGVPGERGGWRQLAAPALGVLVGLVVAGVLPLKHVSTLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC7</accession>
    <name>Q67NC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1831</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195959.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1831"/>
    <dbReference type="EnsemblBacteria" id="BAD40816">
        <property type="protein sequence ID" value="BAD40816"/>
        <property type="gene ID" value="STH1831"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1831"/>
    <dbReference type="eggNOG" id="COG3290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_1_9"/>
    <dbReference type="OMA" id="LEAKYTF"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="178"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="235"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="370"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="687" mass="75407" checksum="0ACD83B78FCE3893" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC9</accession>
    <name>Q67NC9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable butyrate kinase</fullName>
            <shortName evidence="8">BK</shortName>
            <ecNumber evidence="8">2.7.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Branched-chain carboxylic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">buk</name>
        <name evidence="10" type="ordered locus">STH1829</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + butanoate = ADP + butanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:13585"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58079"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.7" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195957.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1829"/>
    <dbReference type="EnsemblBacteria" id="BAD40814">
        <property type="protein sequence ID" value="BAD40814"/>
        <property type="gene ID" value="STH1829"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1829"/>
    <dbReference type="eggNOG" id="COG3426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048716_0_0_9"/>
    <dbReference type="OMA" id="GISICPL"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047761">
        <property type="term" value="F:butyrate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00542">
        <property type="entry name" value="Butyrate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011245">
        <property type="entry name" value="Butyrate_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060:SF3">
        <property type="entry name" value="PTHR21060:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036458">
        <property type="entry name" value="Butyrate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02707">
        <property type="entry name" value="butyr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="358" mass="38937" checksum="D6AA392190EE4B0C" modified="2004-10-11" version="1">MSAEGFRILVINPTATATRIALFDDDEERLAADLAHSPDELARFERVWDQYLYRKDRITERLQAEGIRLESLHAVVGRGGLLKPVPGGVYRVSGAMLDDLRAGVQGEHASNLGGILAYGIAHALGIPAFVVDPVSTDEMWPEGHLTGIPQIRRTSLAHALNMRWVGRRAAAALGKTYDESNLVMAHLGAGISVAAHRQGRMVDATNALEDGPFGPERAGRLPAGDLVRLCFSGRYTERELIRRLTSGGGLVAHLGTRDWREVEKRIAAGDGEARLVYQAMAYQVAKEIGAMAVALRARADAVVLTGDLAHSRLLTDWITGWVDWIAPVLRFPGSEENRALAAGALRVLRGEEEARTYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG7</accession>
    <name>Q67NG7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195919.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1791"/>
    <dbReference type="EnsemblBacteria" id="BAD40776">
        <property type="protein sequence ID" value="BAD40776"/>
        <property type="gene ID" value="STH1791"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1791"/>
    <dbReference type="HOGENOM" id="CLU_1601849_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="166" mass="18289" checksum="8CC6F762C2EFBF80" modified="2004-10-11" version="1">MGILDQITGKAIAQQVTEYSEIYGEILIGLHRDVEHLRDDVDRAQQALARAEQKVERLDQQATRLDRQHDALRSEVQQAIAQFQGLVGCLEGAEAELVSLRTAVARLDPTPAGHPEDGEPGSSGLSVGALWRELEVQRRRVRTLVFLCLLSLLSSVVLGVITWTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI8</accession>
    <name>Q67NI8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanol dehydrogenase regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">moxR2</name>
        <name evidence="2" type="ordered locus">STH1770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195898.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1770"/>
    <dbReference type="EnsemblBacteria" id="BAD40755">
        <property type="protein sequence ID" value="BAD40755"/>
        <property type="gene ID" value="STH1770"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1770"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_9"/>
    <dbReference type="OMA" id="FMARVSI"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="35"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="34614" checksum="B447779094EB0983" modified="2004-10-11" version="1">MEQMAALAARIRANMEKVMVGKADVIELLLVALAAGGHVLIEDVPGTGKTMLAKSLARSLDVTFRRIQFTPDLLPSDVTGLSYFNQKTGEFEFRPGPVFAQVLLADEINRATPRTQSALLECMEERQVTVEGVTRPLARPFLVLATQNPVEQEGTFPLPEAQLDRFLLKLPAGYPSADEEMAMLDRFRSGDPYRELAPVATADEILALQQAVPQVHVARPVQQYIVDLARATRSAEGIELGVSPRGTLALLRASQALAAVRGRDYVLPDDVQYLARPVLAHRLVTSTQLRLRGRGAAEVLDGILAATPVPAEPVPKGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL7</accession>
    <name>Q67NL7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1741</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1741"/>
    <dbReference type="EnsemblBacteria" id="BAD40726">
        <property type="protein sequence ID" value="BAD40726"/>
        <property type="gene ID" value="STH1741"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1741"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_4_9"/>
    <dbReference type="OMA" id="RIVFVYY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="4">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="5">
        <location>
            <begin position="118"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="20318" checksum="842B74EE80DC9036" modified="2004-10-11" version="1">MRPLHETELIRRAQRGDSAALATLLQQHYLPVKKYLVTITFDRALAEDLTQETMIRAIERVGQFQGRSRFSTWLMSIATRQYLDWLRRERRERQAAERAADERPPAQPGPSAEGRTALELLQTLPPEVALPVVLKHYYGYTYEEIAEWMEIPVGTVKSRVFNGVRALRRGLVQDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP0</accession>
    <name>Q67NP0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195846.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1718"/>
    <dbReference type="EnsemblBacteria" id="BAD40703">
        <property type="protein sequence ID" value="BAD40703"/>
        <property type="gene ID" value="STH1718"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1718"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_808727_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269285" evidence="1">
        <location>
            <begin position="21"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="140"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="33821" checksum="E573C0CDAC0F6A31" modified="2004-10-11" version="1">MRCSVWLAALALLIALPAAAANGAASAGPLAEAVSVRALRFGDAGPAVAELQRLLAANGFDPGPVDGIFGPLTEAAVLAAQRHYGLTVDGLAGRMTVGALQSRPQAPVTAAAYDVPERGEGALVVYEASPLAAPRGGDAGTVALTFHHLPAAEELSALLDALRAQGARATFFVTGDEAERRPADLGRIRADGHEIGSLGYREVDLRDRPAVAVRLQLRRASQAIERAVGERPALLRPPLGRFDRQLRRLAAEEGLDLVLWSNAVATPGAGAGPVQLADHALRALHPGAVLMLPLDVPGGVEAAKRVLAGIAAAGYRAVPQEPGVIDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX6</accession>
    <name>Q67NX6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195761.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1632"/>
    <dbReference type="EnsemblBacteria" id="BAD40617">
        <property type="protein sequence ID" value="BAD40617"/>
        <property type="gene ID" value="STH1632"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1632"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_725459_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="381" mass="40598" checksum="8312F5D6AF3645B3" modified="2004-10-11" version="1">MSRAQAIVVRGQEVLMVRHRQGEAEWWCLPGGAVLDGETPAEAALRELEEECRVRGRVVRETSVVTYGPDDRHYTYLVDIGRQEPALGCDPEHAADSQVLQEVRWMRLDALSERDRAFLWTAGLLSVPPFGGLVLGWGDAVSYPCSNALGSAGHIATGRYGQEAGSLGSSSPSLRGNGDAGGTALRIIEHRRHTMRAKPGRHLSQPGVDLARRVGEGLGPFHRVVTSSLPRAFETALAMGFAVDEQVSLLASLPDGFEDEVPWDAGIGRIASAVRRRPDGTVARFAHQLAAFHRDLAARLPDGGRALVISHGGIVEASAVGCKPDGDYAGWGPAFGYCEGVRMYFHGTRCERIEPLRVAAAADPGGQDGAAGGTGDPARGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P11</accession>
    <name>Q67P11_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NADH dehydrogenase I subunit L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nuoL1</name>
        <name evidence="11" type="ordered locus">STH1597</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1597"/>
    <dbReference type="EnsemblBacteria" id="BAD40582">
        <property type="protein sequence ID" value="BAD40582"/>
        <property type="gene ID" value="STH1597"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1597"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018393">
        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="621"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="10">
        <location>
            <begin position="70"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="9">
        <location>
            <begin position="128"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="640" mass="68149" checksum="CB2081DE563DC8C0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P70</accession>
    <name>Q67P70_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chemotaxis signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheC</name>
        <name evidence="3" type="ordered locus">STH1538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195668.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1538"/>
    <dbReference type="EnsemblBacteria" id="BAD40523">
        <property type="protein sequence ID" value="BAD40523"/>
        <property type="gene ID" value="STH1538"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1538"/>
    <dbReference type="eggNOG" id="COG1776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087860_2_0_9"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
        <property type="entry name" value="CheC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04509">
        <property type="entry name" value="CheC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC" evidence="2">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="20976" checksum="2B6C2C8A11632DE6" modified="2004-10-11" version="1">MKLQPSQWTALEQMVTQALAESSRSLSQMTTGEIRLDAPQLSFVPLRELPGVAGGPDHVVTAVYVGIEGELQGHVILLFQPKEARALVDLLLGHPAGTCRELDELGRSALAEVGNICGSAFMNAIADRSGLEVKPTPPVLLEDMAGAILQTVATDLLLTGDEALVIATGFNGAVPGHFLLMPNTESMARLITTLEAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA2</accession>
    <name>Q67PA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1506</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195636.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1506"/>
    <dbReference type="EnsemblBacteria" id="BAD40491">
        <property type="protein sequence ID" value="BAD40491"/>
        <property type="gene ID" value="STH1506"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1506"/>
    <dbReference type="eggNOG" id="COG0803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016838_1_1_9"/>
    <dbReference type="OMA" id="DPHIWFD"/>
    <dbReference type="OrthoDB" id="1703187at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006129">
        <property type="entry name" value="AdhesinB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00691">
        <property type="entry name" value="ADHESINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269296" evidence="4">
        <location>
            <begin position="20"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="309" mass="32925" checksum="E1681F3E2EF53459" modified="2004-10-11" version="1">MVARLRFGLVLLALGIALAAAGCGAGRTGTAAGERIPVVATTGMVADLVRNIGGERVAVTALMGPGVDPHLFKPTEGDVARLQQARIIFYNGLHLEGRMGDILVKMAREKPTVAVAERIPEERLLITEDGVEDPHIWFDVSLWMAAAEVVRDALAELDPDGQSTYEQNARAYLDELAELDEWVRAELATVPAARRVLVTAHDAFGYFGRAYDVEVAGLQGISTAAEYGLADLERTIDLLVGRQIKAVFIESSVPRSAIEALVAGAAARGHAVTIGGELFSDAMGAEGTPEGTYVGMIRHNVRTIADALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA4</accession>
    <name>Q67PA4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1504"/>
    <dbReference type="EnsemblBacteria" id="BAD40489">
        <property type="protein sequence ID" value="BAD40489"/>
        <property type="gene ID" value="STH1504"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1504"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_3_0_9"/>
    <dbReference type="OMA" id="HFVKDEI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="19"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="32863" checksum="F76DA99A35AE6F1D" modified="2004-10-11" version="1">MGGLAVAEELGWRWRAVAGTSAGALVAALVAAGYRAQELREIVRDLDLRSFGDAAGLKVASLLLHEGMFKGEKLLAEVDRLLSDRLGRPRVTFRDLPVACQIVATDLTHRRMLVFPRDLARPPYSMEDPYDFPVADAVRASTAIPFFFQPYRLELPNGVRATLVDGGLLSNFPVHLFQPIGGPACLPTFGFDLRGGDDLPQPTDTPLELVQAMVNTILSARDLSARENQDYVRIIPIETAPYRTTQFDIDADGKEELYRRGRAAAEAFFADPETQRWLDQFAVRRALRARRLPLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE3</accession>
    <name>Q67PE3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ffh</name>
        <name evidence="9" type="ordered locus">STH1465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195595.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1465"/>
    <dbReference type="EnsemblBacteria" id="BAD40450">
        <property type="protein sequence ID" value="BAD40450"/>
        <property type="gene ID" value="STH1465"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1465"/>
    <dbReference type="eggNOG" id="COG0541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_9"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="8">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="189"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="247"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="454" mass="50473" checksum="E095E83F87360481" modified="2004-10-11" version="1">MFESLQERLQEAFKRLRGKGKLTESDVNEALREVRLALLEADVNFKVVKSFIARVKERAVGQEILESLNPAQMVIKIVYEELVALMGGESVGLNMADRPPTIIMLCGLQGAGKTTHAAKLALKLKKEGKKPLLVAADVYRPAAIKQLEVLGEQVGVPVYSEGDQASPVDIAQHAVAYAAEERRDVIIVDTAGRLTIDEELMEELQQIKARIQPHDTLLVLDAMIGQDAVTTAEHFHTKLGIDGVILTKLDGDTRGGAALSVREVTGRPIKFVGVGEKLDALEPFYPDRLASRILGMGDVLTLIEKAQEQVDRKQAEEMARKMLKAQFDFEDFLEQLRALQRMGPLQDILKMLPGVGAQLKDINIDEKELKHIEAIILSMTPKERRNPDIINVRRRERIARGSGRPIAEVHRLIKQFEQTRKLMKQLGGMEKMLKRGGKLPRLPGGGFGGLFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI1</accession>
    <name>Q67PI1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1427</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195557.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1427"/>
    <dbReference type="EnsemblBacteria" id="BAD40412">
        <property type="protein sequence ID" value="BAD40412"/>
        <property type="gene ID" value="STH1427"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1427"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_9"/>
    <dbReference type="OMA" id="TKTQHRF"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="19"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="350"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="383"/>
            <end position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="590" mass="64223" checksum="5AE078866D1AAB09" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR0</accession>
    <name>Q67PR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195478.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40333">
        <property type="protein sequence ID" value="BAD40333"/>
        <property type="gene ID" value="STH1348"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1348"/>
    <dbReference type="HOGENOM" id="CLU_2107751_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="12536" checksum="41CE4AD1A74BC07A" modified="2004-10-11" version="1">MNHTPVSFRVTASDRDEERLAIEIRGTPAGEAVIRYGSGTATVDARVQLSEFPQVIMALDRFHHEFAAELLAYVLDRGAMAQESDGSIIYDLGSALHAVPLPANHEVGLGYPNSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q02</accession>
    <name>Q67Q02_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional protein PyrR</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="5">Pyrimidine operon regulatory protein</fullName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="5">Uracil phosphoribosyltransferase</fullName>
                <shortName evidence="5">UPRTase</shortName>
                <ecNumber evidence="5">2.4.2.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrR</name>
        <name evidence="7" type="ordered locus">STH1256</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer and homohexamer; in equilibrium.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1256"/>
    <dbReference type="EnsemblBacteria" id="BAD40241">
        <property type="protein sequence ID" value="BAD40241"/>
        <property type="gene ID" value="STH1256"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1256"/>
    <dbReference type="eggNOG" id="COG2065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094234_2_1_9"/>
    <dbReference type="OMA" id="PVSANYV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01219">
        <property type="entry name" value="PyrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023050">
        <property type="entry name" value="PyrR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 5" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="6">
        <location>
            <begin position="14"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PRPP-binding" evidence="5">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="183" mass="20505" checksum="BE694482AFA70EDE" modified="2004-10-11" version="1">MTKPEPKAILMDANQMRRALSRIAHEIIERNKGTEHVALVGIRRRGIPLAQRLAERLKEFEGRDVPVGSLDITLYRDDLTELGTLPVVHKSEVNFTLPGRVVVLVDDVIYTGRTVRAALDALLDIGRPSAIQLAVLVDRGHRELPIRPDYVGKNVPTSRRERVEVNVVEIDGEDYVALFDNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q20</accession>
    <name>Q67Q20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1238"/>
    <dbReference type="EnsemblBacteria" id="BAD40223">
        <property type="protein sequence ID" value="BAD40223"/>
        <property type="gene ID" value="STH1238"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1238"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029469_2_1_9"/>
    <dbReference type="OMA" id="GHHDVQP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="193"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="457" mass="49822" checksum="0BBF7FD7750B6695" modified="2004-10-11" version="1">MQQVEAYLRERRDEHLRQLMDFLRIPSVSALSEHRSDVRRAAEWLAAELRRIGLNRVEVMETGGHPVVYAERLDNPGGPTALIYGHYDVQPVDPIELWTTPPFEPDIRDGKLYARGASDDKGQVFMHLKVIEALLAAEGRLPVNVKLLIEGEEEVGSENLERFIAAHRDLLKADVVVISDTALYARGVPSLTYALRGLAALEVHVIGAKGDLHSGAFGGAAPNAAQALVQLLATLHRPEGGVAVAGFYDQVRPLTPEEREGFAALGFDEEKLKADLGVDALPGEPGYTALERLWARPTLEINGIFGGFQGEGTKTVIPARAGAKITCRLVPDQDPERVLDAIEAHLKAHCPAGVRLEIRRMGGTPAAITPIDHPAIRAAMQALSDAYGAEARFIRTGGSIPVVGTFGAVLGTPCVLMGFSLEEENFHAPDEHFHLENFDLGMRALSRFWHYLAEGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q23</accession>
    <name>Q67Q23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sugar ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1235</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195366.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1235"/>
    <dbReference type="EnsemblBacteria" id="BAD40220">
        <property type="protein sequence ID" value="BAD40220"/>
        <property type="gene ID" value="STH1235"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1235"/>
    <dbReference type="eggNOG" id="COG3845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_0_9"/>
    <dbReference type="OMA" id="VGREMSH"/>
    <dbReference type="OrthoDB" id="551294at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="255"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="500" mass="55002" checksum="6DDB68F611719BC7" modified="2004-10-11" version="1">MLLRLEGITKRFGSLVANDHIDLEIRQGEIHALLGENGAGKSTLMNILYGLYTPDEGRIFWKGKEVQIRNPRHAIDLGIGMVHQHFMLVPVFTVAENITLGKEVVRNRFLDMNEARRQVAELSRRFGLEVNPDDRIESISVGLQQRVEILKALYRGAELLILDEPTAVLTPQEVEELGKIMKRLVAEGHTIIIITHKLKEVLHLADRVTVIRRGKKIATLDAAGQTEDELARLMVGRDVILRVDKQPANPGKVVLEVNDLHAKNDRGVDAVRGVSFTVRAGEIVGIAGVDGNGQSELISCITGLRKPTAGTIRVNGQDVTGQSPRRIRDAGVGHIPEDRQKRGLVLNFTVAENAALGDHRAFARRGFISQQQMVQKAQEIISGFDVRPPRHDYLARALSGGNQQKVIVGREILRNPDLLVAVQPTRGLDVGAIEFIHKHLVAERDAGRAVLLVSLELDEIMNLADRILVMYKGQIVAEVPGHEATEEGLGLLMMGGSASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q39</accession>
    <name>Q67Q39_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">ftsZ</name>
        <name evidence="8" type="ordered locus">STH1219</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195350.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1219"/>
    <dbReference type="EnsemblBacteria" id="BAD40204">
        <property type="protein sequence ID" value="BAD40204"/>
        <property type="gene ID" value="STH1219"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1219"/>
    <dbReference type="eggNOG" id="COG0206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_2_1_9"/>
    <dbReference type="OMA" id="GNPSIGQ"/>
    <dbReference type="OrthoDB" id="1009041at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="6">
        <location>
            <begin position="13"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="7">
        <location>
            <begin position="207"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="21"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="108"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="354" mass="36800" checksum="045E23B40208162F" modified="2004-10-11" version="1">MNDYEPGFEQLACIKVIGVGGGGNNAVNRMISAGLQGVEFIAVNTDAQALKSAQAPTRLQIGAKLTKGLGAGADPEIGNRAAEESREEIAAALRGADMVFVTAGMGGGTGTGAAPVVAEIAKELGALTVGVVTRPFTFEGKKRAMQADKGIQNLRQKVDTLITIPNDRLLQVVDKKTSLMEAFRVADDVLRQGVQGISDLIAVPGLINLDFADVRTIMSNTGSALMGIGVGRGESRAADAARAAISSPLLETTIDGAKGVLLNITGGPDLGLMEVNEAAEIIAQAADPEATIIFGAVIDESIQDEIRVTVIATGFGDGDFPAWGRRRAGQNAVGEVKPMVDELDIPAFLRRKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q59</accession>
    <name>Q67Q59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1199"/>
    <dbReference type="EnsemblBacteria" id="BAD40184">
        <property type="protein sequence ID" value="BAD40184"/>
        <property type="gene ID" value="STH1199"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1199"/>
    <dbReference type="eggNOG" id="COG3694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071040_1_1_9"/>
    <dbReference type="OMA" id="AIMRLED"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010390">
        <property type="entry name" value="DUF990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06182">
        <property type="entry name" value="ABC2_membrane_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="250" mass="27574" checksum="C2C9A9082357B17B" modified="2004-10-11" version="1">MKANLASALEYRASLVSQVLGMFINNALWVFFWTLYFTRFPVLRGWTLEDVVVLWAAASTSYGLAVGLMGNVRRLPQLIVQGQLDYFLALPKDVLLHVLVSQIRMIHLGDLLFGPVVLLAVVRPTWGQVAVFAAATVLAALVLLGFLILLGSLTFFLGSAEAVTDQVHNALLHFATYPATIFDGGVRVLLYTVLPAAFISTVPVELVRSFSWTGMLWLACAAAAFMGLGIWTFRRGLRRYESGNLMMMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA4</accession>
    <name>Q67QA4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195285.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1154"/>
    <dbReference type="EnsemblBacteria" id="BAD40139">
        <property type="protein sequence ID" value="BAD40139"/>
        <property type="gene ID" value="STH1154"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1154"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_9_5_9"/>
    <dbReference type="OrthoDB" id="1669868at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="21208" checksum="3A431E8F11C4E54F" modified="2004-10-11" version="1">MAIKAVIFDLGGVIFTLGEQGYRREVARRLGLGDALPAAYEEALPALQRGELAEDDLWERLSGRRVPLDAFDDAWEAHFRVNPEMLALAAELRDRGLKTAILSNTQASHVAIMRRMGVLAPFDPVLMSCEVGCRKPEPAIFRLALERLGLPAEQVAFVDDVPEFVAAARSLGIHGIRHTGDVAATRAALLALVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE4</accession>
    <name>Q67QE4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphopentomutase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1114"/>
    <dbReference type="EnsemblBacteria" id="BAD40099">
        <property type="protein sequence ID" value="BAD40099"/>
        <property type="gene ID" value="STH1114"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1114"/>
    <dbReference type="eggNOG" id="COG1015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079842_0_0_9"/>
    <dbReference type="OMA" id="FHINGQI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043094">
        <property type="term" value="P:cellular metabolic compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010045">
        <property type="entry name" value="DeoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21110">
        <property type="entry name" value="PTHR21110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="5">
        <location>
            <begin position="204"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="32735" checksum="FE70B87FC2C933EA" modified="2004-10-11" version="1">MFFIDGLGLGDDDPDRNPLMTAAMPAFRSLLGGRPLWRGAVPFRGADVAAVPTDACLGVPGLPQSATGQTTIFTGRNGAQAIGRHLNAYPTPSLKAILNEHSIFKRVVERGLSATFLNAFRPEFFAWVAAGQPQHPDRRYRPSASTVAALAAGLQVFRDFDQLRRGEAVGFDIDHHLLRELGYDLDPVDPAEAGRRAARVAAQHHFTLYEHFLTDKAGHAQDMAEARRVLERLDGFLAGLLEALPPDHLLVITSDHGNIEELSVKQHTTNPVATIVRGPGAADVAGRIRSLEDIAPAILDVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ7</accession>
    <name>Q67QJ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glutamine ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1061</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1061"/>
    <dbReference type="EnsemblBacteria" id="BAD40046">
        <property type="protein sequence ID" value="BAD40046"/>
        <property type="gene ID" value="STH1061"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1061"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_1_9"/>
    <dbReference type="OMA" id="MTRAGQL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="19"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="218" mass="24039" checksum="50B8E44066DEB033" modified="2004-10-11" version="1">MARFIEHTLNILPVLFEGLGVTLKLTALSALFGTLLGLLAALARISRNPLFTVPATLYVNLFRGTPLLLQLLFIYNALPQLGLLIQPFPAAVLGYSLNSGAYIAEIIRAAIQSIDRGQMEAARSLGMSYGLAMRRIILPQTYRRLLPPMVNELSALTKDTSLASTISILELMKYSSQFVSRTNLAWQTYVWTAAFYLVVTMTLSALASRLEKKLEARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK6</accession>
    <name>Q67QK6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine diphosphokinase</fullName>
            <ecNumber evidence="1">2.7.6.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.6.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195184.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1052"/>
    <dbReference type="EnsemblBacteria" id="BAD40037">
        <property type="protein sequence ID" value="BAD40037"/>
        <property type="gene ID" value="STH1052"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1052"/>
    <dbReference type="eggNOG" id="COG1564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044237_1_1_9"/>
    <dbReference type="OMA" id="ITYCPEG"/>
    <dbReference type="OrthoDB" id="1628990at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036371">
        <property type="entry name" value="TPK_B1-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63862">
        <property type="entry name" value="SSF63862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPK_B1_binding" evidence="6">
        <location>
            <begin position="144"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="22472" checksum="6287CAAD7A410223" modified="2004-10-11" version="1">MAHGGRRRAAIFAAGELLDLDRVRALVGQPDLVICADGGLRHAQALGLRVDLLVGDFDSLDGAALADARAAGMAIVQVPVEKDRTDSQIALEEALRRGADEILLAGGSGTRLDHTLSNVLLLPRCPVPVTMTDGKSVVRVLRDGQTLEVPRAAGAYLSLVPLTPAVTGVTVTGVHWPLGGAALRWGESLGISNRVVDVAARVSVGAGCLLVIQAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK7</accession>
    <name>Q67QK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1051"/>
    <dbReference type="EnsemblBacteria" id="BAD40036">
        <property type="protein sequence ID" value="BAD40036"/>
        <property type="gene ID" value="STH1051"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1051"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_3_9"/>
    <dbReference type="OMA" id="HWEQMST"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="39"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="132"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="22741" checksum="A505E5957BE71100" modified="2004-10-11" version="1">MSWQARPGAQPLPAGDEEGRRIVRRLTAGDEEALGMMMESFGGALLQYAHRLVGDRHAAEEICQDALLKAWQQGEEFDQDGHLKAWLFRVARHRAIDCLRRRRSVVVEAFANQPAERAVHPEAEAERAWLTEAILDALAELPPPYQSVIQMRFFHDMGYREIAECLHIPIGTVKSRLNYGLKGLARILRARRISLDVLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW5</accession>
    <name>Q67QW5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH943"/>
    <dbReference type="EnsemblBacteria" id="BAD39928">
        <property type="protein sequence ID" value="BAD39928"/>
        <property type="gene ID" value="STH943"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH943"/>
    <dbReference type="eggNOG" id="COG0161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_0_9"/>
    <dbReference type="OMA" id="REGLNQH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="469" mass="51526" checksum="A2F17C80229177E9" modified="2004-10-11" version="1">MRRVIIGPGAGFRTGGDHMNLKELDLRHLIHNEVRFRELARKGTTVITRAEGSTIWDIDGRAYLDAQAGMVLVNVGYGRRELGAVAAAQMERLMYYHTYFQYSNEPAVRLAAKLASLAPEGLGKVFFTLGGAESVETAVKIARLYQRARGRADGHKIICLDLGYHGNSLGALSATAFEAHRAYYGPLVPGFVHIPSPDTFEGPFRADDPEAGRKYAALLEERILAEGPETVAAFLAEPILGVGGIIVPPDDYLKHVRRICDKYGVLLILDEVMTGFGRAGTMWACGQFGVVPDLMCTAKGLTSGYLPLGAVLVGDHVIEAIAEADFPFEHGFTYAGHPVSCAVAMENIAILEREGLAERAARMGERLKEALLARDNPYIAEVRGRGLMVAAELVRDRETRERFPEGNRAFRFDVEAGCLREGVITGIAPYRDTLMITPPLVITEAEIDRLVDVYDRVIRQEGDRRRKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX5</accession>
    <name>Q67QX5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH933"/>
    <dbReference type="EnsemblBacteria" id="BAD39918">
        <property type="protein sequence ID" value="BAD39918"/>
        <property type="gene ID" value="STH933"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH933"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2526299_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="84" mass="9033" checksum="C9BEA8B44F169D3B" modified="2004-10-11" version="1">MISGCRPGVERKCHVDGQVHQVEITRIAREVTGRALTANVVALGVLNRLCRLVSDEALVEAVLETVPRGTEAINRKALEAGMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R93</accession>
    <name>Q67R93_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylthioadenosine phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194948.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH815"/>
    <dbReference type="EnsemblBacteria" id="BAD39800">
        <property type="protein sequence ID" value="BAD39800"/>
        <property type="gene ID" value="STH815"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH815"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_9"/>
    <dbReference type="OrthoDB" id="1364220at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="28290" checksum="F0D612AAAED71BA0" modified="2004-10-11" version="1">MVDTVIIGGTAAYHLQFDPGWTERTVETPFGPAGPFRIFAVAGRPVAFLSRHGGEGRLGVTPPFINYRANVWAARALGARRVLSWNSAGSMVRALPPGSLAVVSDLIDWTRRRPDSFGAAARDPAVRDTVVRHPAPPAGHATGPAYAGLFDPDLRARLVQAAAACGHRAAPRAVYAATEGARLETRAEIALLAQAGAELVGMTLAPEVFLARELGMAYGSICWVSNYATGVPFDGPEQRLFGPEVGQLMFRIMLRLLEEEANAYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA4</accession>
    <name>Q67RA4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH804</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194937.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH804"/>
    <dbReference type="EnsemblBacteria" id="BAD39789">
        <property type="protein sequence ID" value="BAD39789"/>
        <property type="gene ID" value="STH804"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH804"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_9"/>
    <dbReference type="OMA" id="ICPSIAI"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="270"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="392" mass="40341" checksum="CC652B0B6746FB7F" modified="2004-10-11" version="1">MREAVIVSAVRTPIGSLGGALSTVKSYELGAAAIAEALRRAGIRGEQVDEVIIGQVLEAGEGQHPARIASLKAGIPYTVPVTGVNKVCGSGLKAVALAAQAIMLGDADVVVAGGQESMSGAPYLVPRARFGYRMGHGQLVDSMIADALTCGWEHVHMGLTAENIAEQFGISREDQDAFAAASQQKAEAAIKAGRFREEIVPVTVPGRKGDTVVDADEHPRFGTTVEALAKLRPAFKEGGTVTAGNASGINDGAAAVVVMSAEKAASLGVRPMAVIRSYAAAGVEPRIMGTGPIPATRKALARAGLTIDRLDLIEANEAFAAQALAVVRGLELDPGKVNVNGGAIALGHPVGASGARILVTLLYEMQRRRSRYGLATLCIGGGQGIAMVVERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB4</accession>
    <name>Q67RB4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tagatose-6-phosphate kinase</fullName>
            <ecNumber evidence="5">2.7.1.144</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + D-tagatofuranose 6-phosphate = ADP + D-tagatofuranose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12420"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58694"/>
            <dbReference type="ChEBI" id="CHEBI:58695"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.144"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-fructose 1-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14213"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.56"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; D-tagatose 6-phosphate degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carbohydrate kinase PfkB family. LacC subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.144" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH794"/>
    <dbReference type="EnsemblBacteria" id="BAD39779">
        <property type="protein sequence ID" value="BAD39779"/>
        <property type="gene ID" value="STH794"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH794"/>
    <dbReference type="eggNOG" id="COG1105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050013_0_2_9"/>
    <dbReference type="OMA" id="VPKTFYK"/>
    <dbReference type="UniPathway" id="UPA00704">
        <property type="reaction ID" value="UER00715"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008662">
        <property type="term" value="F:1-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009024">
        <property type="term" value="F:tagatose-6-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001059">
        <property type="term" value="P:D-tagatose 6-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005988">
        <property type="term" value="P:lactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01164">
        <property type="entry name" value="FruK_PfkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022463">
        <property type="entry name" value="1-PFruKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017583">
        <property type="entry name" value="Tagatose/fructose_Pkinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000535">
        <property type="entry name" value="1PFK/6PFK/LacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03168">
        <property type="entry name" value="1-PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03828">
        <property type="entry name" value="pfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0423">Lactose metabolism</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="6">
        <location>
            <begin position="13"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="33023" checksum="99FF50F2D57930F9" modified="2004-10-11" version="1">MKTITTVALNPAIDRTLVVPGFTAGATNRVASTRVDPGGKGINVARAAQALGAPVQVLGFLGETNAELILNSLSHEQVVHDFLMVPGETRVNLKIVEPQSGQLTELNESGFAVAPEQVDALVRRVEQRLPETAVLVLAGSLPAGVPGTIYRDLIALARQAGVPTILDADGEPMRIALAARPTLIKPNRDEAERLVGRRLDGPDALVQAARQLLHAGPEVVVVSSGADGAGLVTAQEAWWATPPGIRPGSTVGAGDSMVAGLALALSGGLSLAEALRLATAAGTATARLDGTQVCTRDAVDAVLPQVRIAEVKAKGEGGDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB6</accession>
    <name>Q67RB6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH792"/>
    <dbReference type="EnsemblBacteria" id="BAD39777">
        <property type="protein sequence ID" value="BAD39777"/>
        <property type="gene ID" value="STH792"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH792"/>
    <dbReference type="eggNOG" id="ENOG50337P6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136265_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="15719" checksum="410DC376E65BC74C" modified="2004-10-11" version="1">MGNDIRVSCEGGRLSGRFGGAVIGKDVDLTFDGAVLRGRWGGVWNGKDLHFTIRGTEGRGRMGGSVVGWDIALVQDGDRIAGRIGGAVSGADLRLTAGDGRLTGRIGGSVFGKDCDLQVEGVPYLVAAALAAMVYYQMHLDSSNNASSSSGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE1</accession>
    <name>Q67RE1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194900.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH767"/>
    <dbReference type="EnsemblBacteria" id="BAD39752">
        <property type="protein sequence ID" value="BAD39752"/>
        <property type="gene ID" value="STH767"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH767"/>
    <dbReference type="eggNOG" id="COG5322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1007311_0_0_9"/>
    <dbReference type="OMA" id="YEAGCKL"/>
    <dbReference type="OrthoDB" id="1106190at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="307" mass="33881" checksum="2999752ED7AC03BB" modified="2004-10-11" version="1">MKRIVSVSLGSSRRNSVATVSVLGEEVTVERIGTDGSIEQAIALIRELDGKVDAFGMGGIDLYVYAGGRRYAFRDAKRIAAAAVRTPIVDGSGLKNTLERRAVYWLDRNVMPLRGKRVLMVASVDRFGMAEALVDVGADIVFGDMMFALGIPIPIRSFRTIHVLARILLPIITQLPFTWLYPTGAKQETHQPRFTEYYQWADLIAGDWLYIRRYAPDRLEGKTILTNTVTPADIEWMKQAGVATLVTTTPNLGGRSFGTNLMEGLIVAAAKKRADEMSPADYEEWLDRIGFTPRVERPAARGSAAGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG2</accession>
    <name>PSTB1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB 1</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB1</name>
        <name type="ordered locus">STH746</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RG2"/>
    <dbReference type="SMR" id="Q67RG2"/>
    <dbReference type="STRING" id="292459.STH746"/>
    <dbReference type="EnsemblBacteria" id="BAD39731">
        <property type="protein sequence ID" value="BAD39731"/>
        <property type="gene ID" value="STH746"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH746"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="TIAMKPE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015850">
        <property type="entry name" value="ABC_transpr_PstB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF3">
        <property type="entry name" value="PTHR43423:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092911" description="Phosphate import ATP-binding protein PstB 1">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <sequence length="242" mass="26835" checksum="B50BF61F7D42F5C7" modified="2004-10-11" version="1">MDLYYGSYRALRSISLEIPANRVTALIGPSGCGKSSFLRCLNRMNDLIPGARVEGTITLDGADIYAPGVDVVALRRRVGMVFQRPNPFPMSIYDNVAYGPRIHGERRKAVLDEIVEQSLRGAALWDEVKDRLHRSALGLSGGQQQRLCIARSLAVKPEVLLMDEPASALDPISTAKIEELIRELRAQYSIVIVTHNMQQAARISDYTAFFLSGELIEHGPTGTIFTNPKDQRTEDYITGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ1</accession>
    <name>Q67RQ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SNF2 family helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH657</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194790.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH657"/>
    <dbReference type="EnsemblBacteria" id="BAD39642">
        <property type="protein sequence ID" value="BAD39642"/>
        <property type="gene ID" value="STH657"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH657"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_21_8_9"/>
    <dbReference type="OMA" id="FHIDRWW"/>
    <dbReference type="OrthoDB" id="325071at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022138">
        <property type="entry name" value="Helicase_SWF/SNF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12419">
        <property type="entry name" value="DUF3670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="532"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="820"/>
            <end position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="989" mass="107410" checksum="A91BD5C8869DF8BD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ6</accession>
    <name>Q67RQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH652</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194785.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH652"/>
    <dbReference type="EnsemblBacteria" id="BAD39637">
        <property type="protein sequence ID" value="BAD39637"/>
        <property type="gene ID" value="STH652"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH652"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_2_9"/>
    <dbReference type="OMA" id="IWQIRTP"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="36020" checksum="CB35029D2705FA4D" modified="2004-10-11" version="1">MTSRNPRPAPRWRALLLSGLFTGLVILLSFGMGRYPIPPGEVVRILGQIVTGRFDPATATEAARVLFYIRLPRILLAALTGVALSGAGTLFQGMFRNPLVSPDVLGVSSGAALGAAIGILLPAGGSIQLWAFACGVAAVALTYGIAQASRGQSVVMLVLAGMVVSAFFSAALSFLKYVADPYEQLPTIVFWLMGGFYRASWPAVGSLLLTVLPGAVLLLLTSWKLNILSLGDEEALSLGVNVRVLRPLLIAAATLMVAGAVSVTGTISWVGLVVPHIARMLAGADHDRSMPVAMLFGAGFMLLMDDLARSLTTAEIPVGILTSAVGAPFFGYLLISGRREAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT6</accession>
    <name>Q67RT6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194755.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39607">
        <property type="protein sequence ID" value="BAD39607"/>
        <property type="gene ID" value="STH622"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH622"/>
    <dbReference type="HOGENOM" id="CLU_2169789_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="110" mass="12145" checksum="41A20BE3BE3D6DC3" modified="2004-10-11" version="1">MRERLYRAGYPATAAGWTGFLALMAFALALTVVATGVNGLALIGLVGLTWLLNVATVHWAVPWRLVCHVFGWVVLYRSVVPDRAWYLVVPAALLLAGSIIWEQERRLARA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S16</accession>
    <name>CRCB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB2</name>
        <name type="ordered locus">STH542</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S16"/>
    <dbReference type="SMR" id="Q67S16"/>
    <dbReference type="STRING" id="292459.STH542"/>
    <dbReference type="EnsemblBacteria" id="BAD39527">
        <property type="protein sequence ID" value="BAD39527"/>
        <property type="gene ID" value="STH542"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH542"/>
    <dbReference type="eggNOG" id="COG0239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_2_3_9"/>
    <dbReference type="OMA" id="TWMLETH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000110199" description="Putative fluoride ion transporter CrcB 2">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="123" mass="13000" checksum="3DF3107C9DDF11C3" modified="2004-10-11" version="1">MAGVAAGGALGAWARHGLGSWLTALLPSTFPLPTLMINVLGALLLGFVAGYGIERGRLPEAWRLPVTVGFIGSFTTFSTWSVDTVLLLDAGRWPLALANVGISLAVGLAAVWVGRRLAFRWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S32</accession>
    <name>Q67S32_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGA_cap domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH526</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CapA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194660.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH526"/>
    <dbReference type="EnsemblBacteria" id="BAD39511">
        <property type="protein sequence ID" value="BAD39511"/>
        <property type="gene ID" value="STH526"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH526"/>
    <dbReference type="eggNOG" id="COG2843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041663_0_0_9"/>
    <dbReference type="OrthoDB" id="1003186at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07381">
        <property type="entry name" value="MPP_CapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019079">
        <property type="entry name" value="Capsule_synth_CapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09587">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00854">
        <property type="entry name" value="PGA_cap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269344" description="PGA_cap domain-containing protein" evidence="3">
        <location>
            <begin position="32"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="PGA_cap" evidence="4">
        <location>
            <begin position="213"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="516" mass="55184" checksum="D2053B190628F020" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S51</accession>
    <name>PRMA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein L11 methyltransferase</fullName>
            <shortName evidence="1">L11 Mtase</shortName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prmA</name>
        <name type="ordered locus">STH507</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methylates ribosomal protein L11.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194641.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S51"/>
    <dbReference type="SMR" id="Q67S51"/>
    <dbReference type="STRING" id="292459.STH507"/>
    <dbReference type="EnsemblBacteria" id="BAD39492">
        <property type="protein sequence ID" value="BAD39492"/>
        <property type="gene ID" value="STH507"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH507"/>
    <dbReference type="eggNOG" id="COG2264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049382_0_1_9"/>
    <dbReference type="OMA" id="EFFFIFP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00735">
        <property type="entry name" value="Methyltr_PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004498">
        <property type="entry name" value="Ribosomal_PrmA_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000401">
        <property type="entry name" value="RPL11_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00406">
        <property type="entry name" value="prmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000192322" description="Ribosomal protein L11 methyltransferase">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00735"/>
        </source>
    </evidence>
    <sequence length="304" mass="32911" checksum="70E96569508B0A03" modified="2004-10-11" version="1">MRYLEIRIRCQRAAADAVGNLLLTLTGAGYAVDDPLIVEQNRSRWDMTDLPPGDPEWVTVSGWLPEAGDVEQQRLRLETGLDEIRSLGLGAVDPARFRWVEEEDWAHAWKAYFRPTRVGDRLVVVPAWEEYAPQEGELPIRIDPGMAFGTGTHATTALCMRWLEELVTPGSRVIDVGTGSGILAVAAKHLGAAEVVAIDVDPVAVDAARENAGRNGVEIDVRLATLDQVAEGEADLIVANIIASVIVDILPDVASRLKPGGRFLASGIIAARKEAVTEAMTDAWLLPVGAREQDGWVAILAMKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S79</accession>
    <name>Q67S79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194613.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH479"/>
    <dbReference type="EnsemblBacteria" id="BAD39464">
        <property type="protein sequence ID" value="BAD39464"/>
        <property type="gene ID" value="STH479"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH479"/>
    <dbReference type="HOGENOM" id="CLU_2557037_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="9005" checksum="E957C17B4E77EFB1" modified="2004-10-11" version="1">MTLHEVAAELARRMNCTVEPAHGDAQSVTVRGKGYHFVVAGFFGGWQATLYLPDQDPVTFYGEAVEALEIRLKGRLSGRPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH6</accession>
    <name>Q67SH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tungstate ABC transporter ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH382</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194516.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH382"/>
    <dbReference type="EnsemblBacteria" id="BAD39367">
        <property type="protein sequence ID" value="BAD39367"/>
        <property type="gene ID" value="STH382"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH382"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="HVYCCIR"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="27717" checksum="2B87716C7129F4F7" modified="2004-10-11" version="1">MTALLEVTDVKVKRGNRVVVDVPHLAIQPGEVLAVIGPNGSGKSSLLHALALLIPASFGSYRWEGRQVSLPQEALALRRQMAVVFQESLLLTGTVMQNVTLGLRLRGVPAAKQREVAMEMLEQLRISHLAHRSSRQLSGGEAQRVSIARALATGPRLLYLDEPMASLDVLARSHLLADLRRILTASGTAALFVTHDFTEIPPLADRVAVLAEGKLLQIGTPAEVFNQPATPLVRDLVQVAHDLVRTLDAGRRQEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR5</accession>
    <name>Q67SR5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH293"/>
    <dbReference type="EnsemblBacteria" id="BAD39278">
        <property type="protein sequence ID" value="BAD39278"/>
        <property type="gene ID" value="STH293"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH293"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453970_0_0_9"/>
    <dbReference type="OMA" id="SMESKWI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="78"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="600" mass="67509" checksum="CF251875468074E8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU9</accession>
    <name>Q67SU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Asparagine synthase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194394.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH259"/>
    <dbReference type="MEROPS" id="C44.976"/>
    <dbReference type="EnsemblBacteria" id="BAD39244">
        <property type="protein sequence ID" value="BAD39244"/>
        <property type="gene ID" value="STH259"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH259"/>
    <dbReference type="eggNOG" id="COG0367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_2_2_9"/>
    <dbReference type="OMA" id="IEHSHQP"/>
    <dbReference type="OrthoDB" id="610569at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="7">
        <location>
            <begin position="2"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="326"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="6">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="506" mass="55614" checksum="95BC057E7BBE9FAE" modified="2004-10-11" version="1">MSGIAGILGKPDKARIVRMLEQQAHRGPDGRGIWTATAGFGLGHVRLATVDVAGGGQPLSNEDGSLWLAMTGAIYNHGALRRELEGRHAFRTQSDAEVVVHLFEEYGPACVERLDGMFAIAIWGEEVGLFLARDPLGIQPLYWGEDEEGNVLFASEIKALVGETRFVREFPPGHRWRAGEPLEPYERLPAHTGELADPDQIVLALDRLLHAAVRKQLAADVPVGCLLSGGLDSSLVTAIARQHAEGELHTFAVGLEGSADLEQARMVAGELGTIHHERVLTEKEVTAVLPRVVDALESCDPALVRSAVATYYVAELAAGHVKVVLSGEGADELFAGYDYLAEFERDAGALGAELYEITAAMHNCNLQRVDRMTQVHGVEARMPFADQAVVDFAFRIHPRLKRRDGESKWILRRVAEQYLPPAIVWREKQKFAIGSGIAPLLEHYAAKAVPDAAFRRARSPKGVPFASKEEFLYWSLFRERYGREDVLALMGRSRSLNQGQRWVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY2</accession>
    <name>Q67SY2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uracil permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">uraA</name>
        <name evidence="8" type="ordered locus">STH226</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194361.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH226"/>
    <dbReference type="EnsemblBacteria" id="BAD39211">
        <property type="protein sequence ID" value="BAD39211"/>
        <property type="gene ID" value="STH226"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH226"/>
    <dbReference type="eggNOG" id="COG2233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_1_2_9"/>
    <dbReference type="OMA" id="FGLCPKF"/>
    <dbReference type="OrthoDB" id="1115239at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="410" mass="41733" checksum="1A41A9B003091B3D" modified="2004-10-11" version="1">MAGSTGTLSPTKRFVLGLQHVAAMFGATVLVPIQTGLNPSVALLTAGLGTLLFHLVTKGRVPVFLGSSFAFIAAIQLVGQTEGLEYAAGGIVAAGLVYLVLSGLIYLFGVDRIRSFFPPIVAGPMIMVIGLTLAPVAVDMASSHWLVALITLLTVVLVSVYARGFLKLLPIICGLLVGFAASAAFGLVDWAAVAQASWVAVPPVTAPKFSLSAILAIAPLALVTMVEHIGDITTNGAVIGKDLIRDPGLHRTLMGDGLATALAGLLGGPANTTYSENTGVLAVTRVYDPGILRIAAVVAIALSFVGKLGALLQAIPTPVMGGISIVLFGMITSIGIRQVVDARVDLTNGRNLVIAAVVLVLGIGGAALPLPGGLQLGGMALAALVGVVLNKLLPEQVVRETEPALTGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T05</accession>
    <name>Q67T05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Stage V sporulation protein AD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH203"/>
    <dbReference type="EnsemblBacteria" id="BAD39188">
        <property type="protein sequence ID" value="BAD39188"/>
        <property type="gene ID" value="STH203"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH203"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048574_0_0_9"/>
    <dbReference type="OMA" id="GHQTWVF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010894">
        <property type="entry name" value="SpoVAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038369">
        <property type="entry name" value="SpoVAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07451">
        <property type="entry name" value="SpoVAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011570">
        <property type="entry name" value="SpoVAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02845">
        <property type="entry name" value="spore_V_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="338" mass="35944" checksum="488B5B6BFF6DE81A" modified="2004-10-11" version="1">MARKHIGAQTVRLEQPPVIVGAAAVAGPVEAQGPLGHEFDLTYPDLTVGQDSFEKAERQMMIDACNLALRRAGIQQKPPAVDYFLAGDLLNQLITTAFSGLELGVPLYGLYGACSTFTEALSLGAKLIDGGFADRVLCAVSSHYGSAERQYRYPTEYGSQRKPYAQHTVTGAGAVVLASAGEGPRITYCTTGKVVDWGVADPLNQGAAMAPAAVDTIEAHLRDTGRSVDDYDLIVTGDLAKFGYEMARRLAAERNLYLGENYRDCGVMVYDTTKQNVDSGGSGCASSAIVTCGHLLKEMARGRLRRLLVVSTGSLFSPTSFQQGESIPTIAHAVAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T09</accession>
    <name>Q67T09_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TerC family membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH199</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TerC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39184">
        <property type="protein sequence ID" value="BAD39184"/>
        <property type="gene ID" value="STH199"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH199"/>
    <dbReference type="eggNOG" id="COG0861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045644_1_2_9"/>
    <dbReference type="OMA" id="ADHAREY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022369">
        <property type="entry name" value="Integral_membrane_TerC_rswitch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30238:SF0">
        <property type="entry name" value="PTHR30238:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03718">
        <property type="entry name" value="R_switched_Alx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="310" mass="34752" checksum="5CB7B14776A9E16F" modified="2004-10-11" version="1">MHNLELTAFFLFVIFATWLDLFVLHKDDHDIGLKEATVTSLAWILLAVLFSGYVYYRYGTEMWMQYITAYTMEKALSVDNLFVIATIFGFFGIRGGLQHRALAWGVIGAVAMRSLLIFLGVEAVERFSWLLPVFGVFLVVTGFKMLRENDEEKASLEDNKVYRLLSRVLPVYPGYDGHAVITRRNGRWELTLLGMAIVMVEATDLLFAVDSIPAVMAISQDFFIVLTSNIFAILGLRALYFLLAGILGKFRYLSLGLAFVLLFIGGKMLVLPFGIHVETWISLSVVLGTITIAILASLLIPEKTRPESAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T48</accession>
    <name>CPFC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coproporphyrin III ferrochelatase</fullName>
            <ecNumber evidence="1">4.99.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cpfC</name>
        <name type="ordered locus">STH160</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Fe-coproporphyrin III + 2 H(+) = coproporphyrin III + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:49572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:68438"/>
            <dbReference type="ChEBI" id="CHEBI:131725"/>
            <dbReference type="EC" id="4.99.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:49574"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194295.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T48"/>
    <dbReference type="SMR" id="Q67T48"/>
    <dbReference type="STRING" id="292459.STH160"/>
    <dbReference type="EnsemblBacteria" id="BAD39145">
        <property type="protein sequence ID" value="BAD39145"/>
        <property type="gene ID" value="STH160"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH160"/>
    <dbReference type="eggNOG" id="COG0276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_0_0_9"/>
    <dbReference type="OMA" id="FSYHGVP"/>
    <dbReference type="OrthoDB" id="780534at2"/>
    <dbReference type="UniPathway" id="UPA00252"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="PTHR11108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000175214" description="Coproporphyrin III ferrochelatase">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <sequence length="320" mass="36453" checksum="0A7886E5D1D5423C" modified="2004-10-11" version="1">MEKTAVLLVNLGTPSAPEPDAVRPYLAEFLGDWWVIDKPRWQWLPILHGIILRVRPPRVAKIYQKIWLPEGSPLLHYSRLQQAALQQRLEPEGIRVALGMTYGQPSVKSALEELRGWGVRRLLVLPLFPQYSSTTTAAVWSKVQKALDGWRDLPEQIFIRDFPTHPKYLAFLTERISGYIAEKGRPDALVLSYHSIPQAYTASGDDYAAQCESTTDAVRDRFPDLKIVMGYQSKFGNDPWLEPATDEVLRELVRTGHRHVAVMAPGFAADCIETLHELEVEYAEEFVKAGGERYDYLPAANDHPLFIDCLEDLVRRHLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T59</accession>
    <name>Q67T59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH149"/>
    <dbReference type="EnsemblBacteria" id="BAD39134">
        <property type="protein sequence ID" value="BAD39134"/>
        <property type="gene ID" value="STH149"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH149"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_9"/>
    <dbReference type="OMA" id="RAWWVVS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="99"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="302" mass="32872" checksum="510D83B8B70033CC" modified="2004-10-11" version="1">MAAPHLNVEAEEQLQGLAREESQFRQFARRFARNKLAVAGLVILVILYAVAIFAPFLASEPLDARVNLTERFLPPGSPGHPLGTDNLGRDLLSRMIWGSRISLSVGFVAMGVAVLLGTLVGSIAGYYGGTWIDIVLMRFAEAIDVIPVFFLLVTVVSVLEPSVYNIMIVIGLTSWPGLAYIVRGQFLSLRQRDFTEASRAMGATDMRLIFRHILPNAVAPIIVSATLRIGNAILSEAGLSFLGLGAPPPAVSWGQILSVSRTFLKQAPWLMFWPGLAIFVTVLAFNFVGDGIRDALDPKLKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T86</accession>
    <name>FLIW_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flagellar assembly factor FliW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fliW</name>
        <name type="ordered locus">STH122</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin. Binds to flagellin and participates in the assembly of the flagellum.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with translational regulator CsrA and flagellin(s).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FliW family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194257.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T86"/>
    <dbReference type="SMR" id="Q67T86"/>
    <dbReference type="STRING" id="292459.STH122"/>
    <dbReference type="EnsemblBacteria" id="BAD39107">
        <property type="protein sequence ID" value="BAD39107"/>
        <property type="gene ID" value="STH122"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH122"/>
    <dbReference type="eggNOG" id="COG1699">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112356_0_2_9"/>
    <dbReference type="OMA" id="DVAVFCI"/>
    <dbReference type="OrthoDB" id="1767625at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01185">
        <property type="entry name" value="FliW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003775">
        <property type="entry name" value="Flagellar_assembly_factor_FliW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024046">
        <property type="entry name" value="Flagellar_assmbl_FliW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39190">
        <property type="entry name" value="PTHR39190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02623">
        <property type="entry name" value="FliW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141457">
        <property type="entry name" value="SSF141457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_0000273007" description="Flagellar assembly factor FliW">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01185"/>
        </source>
    </evidence>
    <sequence length="138" mass="15293" checksum="23685FB0430C7351" modified="2004-10-11" version="1">MDNVFEFPAGLYGFPDLKRFVVVDVPGAGDVVKQLVSTEDPNVGFTLVFPFAFFPRYSPDIPEEELVAVGAESAEQVLLYAIASVPEDFRKATANLRAPVLFNPFTRKGRQVILGDDRYGIREPLFQGADRIPAEEGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB9</accession>
    <name>ATPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">STH89</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194224.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TB9"/>
    <dbReference type="SMR" id="Q67TB9"/>
    <dbReference type="STRING" id="292459.STH89"/>
    <dbReference type="PRIDE" id="Q67TB9"/>
    <dbReference type="EnsemblBacteria" id="BAD39074">
        <property type="protein sequence ID" value="BAD39074"/>
        <property type="gene ID" value="STH89"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH89"/>
    <dbReference type="eggNOG" id="COG0056">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010091_2_1_9"/>
    <dbReference type="OMA" id="LQAPGVM"/>
    <dbReference type="OrthoDB" id="837522at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000238375" description="ATP synthase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="1">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <sequence length="506" mass="55164" checksum="1800C9C29BA86B90" modified="2004-10-11" version="1">MSIRPEEISSILKQEIERFSTEIETSNVGRVIQVGDGIARVYGLQSCMASELLEFPNGIMGMAFNLEEDNIGVVILGPDEDIKEGMEVRRTGRVVEVPVGPALIGRVVNALGEPLDGKGPIETDMYLPVERKAPGVITRKSVHEPLQTGLKAIDSMTPIGRGQRELIIGDRGTGKTAIAVDTIINQKGQNCICIYVGIGQKASTIAGVVKKLQETGAMEYTIVVSATASDPAPLQFLAPYAGCAMGEYFRDNGMHALIVYDDLSKHAVAYREMSLLMRRPPGREAYPGDVFYLHSRLLERAAKMSDEYGAGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETDLFNAGVRPAINVGISVSRVGGAAQIDSMKRVSGTLKLDLAQYRELAAFAQFGSDLDKATQARIIRGQRTTEILKQPQYHPLPVELQVLSIFAATNGYLDDLELEEVAAFEQELHTYFKANHPELLARIPKEKWKDIEPEVRAAVDAFKKGYGQRAAANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE1</accession>
    <name>Q67TE1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fructose-1,6-bisphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH67</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
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            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
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            <dbReference type="EC" id="3.1.3.11"/>
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            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
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        <text evidence="2 3">Belongs to the FBPase class 2 family.</text>
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    <dbReference type="eggNOG" id="COG1494">
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    <dbReference type="OMA" id="AVFYMDK"/>
    <dbReference type="OrthoDB" id="879338at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="P:gluconeogenesis"/>
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            <end position="89"/>
        </location>
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    <feature type="region of interest" description="Substrate binding" evidence="5">
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            <end position="165"/>
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            <end position="187"/>
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    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29035"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="34369" checksum="461F82554E38629A" modified="2004-10-11" version="1">MERELALELVRITEAAALASARWMGRGDKELADQLAVDAMRAGFDRVAIDGVVVIGEGEMDEAPMLYIGEQVGAGGIPVDVAVDPVEGTNLVAKGLNGAIAVVAVAPRGHLLHAPDMYMEKIAVGPRAKGCIDITAPITANLKWVAQAKGKDISDLTVVILDRERHRSLIQECRDAGARIKLISDGDVAPAVAAAIDETGVDILMGIGGAPEGVLAAAALKCLGGDMQARLKPQNEQEYARCKAMGLSDPEQVLTLDDLVKSEDVYFSATGITDGDLLRGVRYSGRNVATTHSIALRGTTGTVRFIEATHMLDRKPHWYRLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG5</accession>
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        </submittedName>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
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                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
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            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    </dbReference>
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        <location>
            <begin position="41"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="2">
        <location>
            <begin position="109"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="1223" mass="128485" checksum="3AC7A606D8F31ABE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK5</accession>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
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                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38988.1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02988">
        <property type="entry name" value="YaaA_near_RecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD38988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="8254" checksum="F99AC96F77FEEA24" modified="2004-10-11" version="1">MAEEIVTIHTEYITLDAFLKWTGVADTGGQAKALIAAGLVHVNGEPELRRGRKLRAGDRVRLEGGGQWLIRREGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J36</accession>
    <name>Q67J36_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ParA, ParA family ATPase</fullName>
        </submittedName>
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        <name evidence="2" type="ordered locus">STH3333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
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        <lineage>
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            <taxon>Clostridia</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    <dbReference type="OMA" id="VQCEYFA"/>
    <dbReference type="OrthoDB" id="729012at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="3"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="28414" checksum="15B66CE736650C94" modified="2004-10-11" version="1">MSRVIAIANQKGGVGKTTTAVNLSACLADLGKRVLLIDVDPQGNATSGLGLDRRRLKASVYDVLLDELPLQEAVVNTRVRGLQVVPATIDLAGAEIELVPRIARESRLKQALDPVRDQYDFVLMDCPPSLGLLTINALTAADSILIPIQCEYYALEGLTQLMDTFRLVREALNPNLEVEGVVLTMFDGRTNLSIQVVEEVKRFFKGQVYRSIITRNVRLSEAPSHGLPITLYDPRSKGAEAYMELAREVIERVEERAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB5</accession>
    <name>Q67JB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sporulation-related transcriptional regulator AbrB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">abrB</name>
        <name evidence="3" type="ordered locus">STH3253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3253"/>
    <dbReference type="EnsemblBacteria" id="BAD42235">
        <property type="protein sequence ID" value="BAD42235"/>
        <property type="gene ID" value="STH3253"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3253"/>
    <dbReference type="eggNOG" id="COG2002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158484_0_1_9"/>
    <dbReference type="OMA" id="VCRECAI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04014">
        <property type="entry name" value="MazE_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00966">
        <property type="entry name" value="SpoVT_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01439">
        <property type="entry name" value="lp_hng_hel_AbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="2">
        <location>
            <begin position="9"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="89" mass="10038" checksum="1574F98D0499B528" modified="2004-10-11" version="1">MRHMMKSTGIVRKVDELGRVVIPIELRRTLNIEEKDSLEIYVDGDKIILRKYEPACVFCGNAANVENFKGKNVCQSCLASMAERAHQVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV4</accession>
    <name>RL24_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplX</name>
        <name type="ordered locus">STH3064</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197179.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV4"/>
    <dbReference type="SMR" id="Q67JV4"/>
    <dbReference type="STRING" id="292459.STH3064"/>
    <dbReference type="EnsemblBacteria" id="BAD42046">
        <property type="protein sequence ID" value="BAD42046"/>
        <property type="gene ID" value="STH3064"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3064"/>
    <dbReference type="eggNOG" id="COG0198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093315_3_0_9"/>
    <dbReference type="OMA" id="HVKPTQE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241671" description="50S ribosomal protein L24">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <sequence length="83" mass="9003" checksum="20F6BFF264492CC1" modified="2004-10-11" version="1">MHVKKDEIVVVITGKDKGKQGKVLRVLPKENRVVVEGVNVVKKHTKPSAKNPNGGIIEMEAPIHASNVMSLAKIEAKKAKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY1</accession>
    <name>Q67JY1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3036</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197151.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3036"/>
    <dbReference type="EnsemblBacteria" id="BAD42019">
        <property type="protein sequence ID" value="BAD42019"/>
        <property type="gene ID" value="STH3036"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3036"/>
    <dbReference type="eggNOG" id="COG5496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119426_0_1_9"/>
    <dbReference type="OMA" id="VFATPAM"/>
    <dbReference type="OrthoDB" id="1895484at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025540">
        <property type="entry name" value="FlK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36934">
        <property type="entry name" value="PTHR36934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014972">
        <property type="entry name" value="FlK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="3">
        <location>
            <begin position="36"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR014972-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR014972-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="130" mass="14047" checksum="667F73466E83AE2A" modified="2004-10-11" version="1">MSLAPGLTATAETVVTPENCARAVGSGAVDVFATPMMIALMEEAAARAVEPFLGEGETTVGTRVDVEHLAATPPGMKVRATAVLERVDGRRLLFRVEAFDDRERVGSGTHERVIVRMERFLQRAGEKRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K56</accession>
    <name>Q67K56_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PepSY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2959</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2959"/>
    <dbReference type="EnsemblBacteria" id="BAD41942">
        <property type="protein sequence ID" value="BAD41942"/>
        <property type="gene ID" value="STH2959"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2959"/>
    <dbReference type="eggNOG" id="COG2959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045803_0_0_9"/>
    <dbReference type="OMA" id="STAYWAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014239">
        <property type="entry name" value="Spore_YpeB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03413">
        <property type="entry name" value="PepSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14620">
        <property type="entry name" value="YPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02889">
        <property type="entry name" value="spore_YpeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269241" description="PepSY domain-containing protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="3">
        <location>
            <begin position="398"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="471" mass="51251" checksum="A9BEBE23E9294C2E" modified="2004-10-11" version="1">MDMRRVVTWLSVSLFAAVAVAVWAAWTANLNEHNRLLANALEAERQRNFVDMVHHVEQIQALLGKGLATGSERQNMRYMADVHRHASAAMTAFSSLPLPTEVSSTTGKFLQQVGDFAYALLRDEAAGRSLTEAERAELQRLREAAAALKEQLGTMLTQYQRGGFRWHQPAQLSLRTLMRPPGRVLSDPAASGGPAAAALLDGGWAQLATSMEQMPTFIYNGPFSDHVNQTPPALSGPQLSREEAGGRLATALPDAAAYRVVDVVESGGNLPAYTFRLAPAGTRGSAYTATVSLTRQGGHLLEFLSGRVLGQPALDLEQARAVGQEYLARIGYADMVPTFAQIQDGVAFVAYVYQQGEVIVYPDQAKVKVALDNGEVLGVDARQYLTNHRRRTLPRPRLTPDEARSRLNPHLEVSRVQLALIPDAAGTGEVLTYEFQGKIGDEVYLVYINAETGAEEQILQLIVTDGGTFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K61</accession>
    <name>Q67K61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2954</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197069.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2954"/>
    <dbReference type="EnsemblBacteria" id="BAD41937">
        <property type="protein sequence ID" value="BAD41937"/>
        <property type="gene ID" value="STH2954"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2954"/>
    <dbReference type="eggNOG" id="COG2309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062630_0_0_9"/>
    <dbReference type="OMA" id="HEDYVFY"/>
    <dbReference type="OrthoDB" id="689314at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000787">
        <property type="entry name" value="Peptidase_M29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02073">
        <property type="entry name" value="Peptidase_M29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="32253" checksum="46D09B6E3830C15A" modified="2004-10-11" version="1">MVPRWAGTVLQDSLGLRPGERVLVLADRPLAPAAAALDEAARALGAAEVKAHLLPDPDRLQVVPRWITDAVRSADAIVSLRAALRLAAEDALMRAALDAFHRARRGRWVSMAQVDVSVLETALAPGLAEAAARARALAARLRAAAGVRLTAPGGTDLQLTFAGRPVHADTGWVRQPGDFGNLPAGEAYVAPDETGAEGLLVVDVALGDIPLDRPVALRFRRGRVAEVTGGEAAAELRRRLGNDPWAWTVGELGLGTNPHVRPCGRVAVAEKALGTAHVALGGNLAFGGRNPASTHYDCVIAAADVRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K68</accession>
    <name>Q67K68_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative cyclic nucleotide-binding transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2947</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197063.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2947"/>
    <dbReference type="EnsemblBacteria" id="BAD41930">
        <property type="protein sequence ID" value="BAD41930"/>
        <property type="gene ID" value="STH2947"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2947"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_3_2_9"/>
    <dbReference type="OMA" id="VLCRDFG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018335">
        <property type="entry name" value="Tscrpt_reg_HTH_Crp-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00034">
        <property type="entry name" value="HTHCRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00042">
        <property type="entry name" value="HTH_CRP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="146"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="24752" checksum="20A811963A2AFC50" modified="2004-10-11" version="1">MPDLDFLRSLSLFQGLSDEELARIAAIARERHYRKGTILFNEGDPGEAIYFIKKGRVRVSRLAANGQEQVLRVWGPGHEVGLVVLADKAPYPATAQVTEDSVLIAFRVDDLVQLLPESRTLAANAFCLVGRRLRLARDTAHELAVHNTHGRLAALLLRMKAEQGSDVINLGMTHQEIAGMIGTSRETVTRVLGDFRRTGCIAVQGESVVIRDERKLREWMGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB0</accession>
    <name>Q67KB0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="6 10">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH2905</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="function">
        <text evidence="3">May be involved in the biosynthesis of molybdopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="6 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197022.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2905"/>
    <dbReference type="EnsemblBacteria" id="BAD41888">
        <property type="protein sequence ID" value="BAD41888"/>
        <property type="gene ID" value="STH2905"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2905"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1910">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_3_0_9"/>
    <dbReference type="OMA" id="IEAKTHS"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9 10" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="11">
        <location>
            <begin position="183"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD41888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="641" mass="69728" checksum="00F33450FEA3B3FE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK0</accession>
    <name>Q67KK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methionyl-tRNA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">metS2</name>
        <name evidence="8" type="ordered locus">STH2813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196932.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2813"/>
    <dbReference type="EnsemblBacteria" id="BAD41798">
        <property type="protein sequence ID" value="BAD41798"/>
        <property type="gene ID" value="STH2813"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2813"/>
    <dbReference type="eggNOG" id="COG0143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1712346_0_0_9"/>
    <dbReference type="OrthoDB" id="761140at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="PTHR45765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anticodon_3" evidence="7">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="17239" checksum="301BD885A45B09B2" modified="2004-10-11" version="1">MPAGGEPGEREQALARECAAAVGEYRSHLRSVEFRKAVQAMRRLCTLGNRYLEDRAPWNLVKEDREAGAMVLRTALNLVRIFALAAEPILPFTAQRVFDALQLTPDERKVPLQRCVDLSALAPGRQFQLIPPLFRRLEAAEVERLKARFGGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK4</accession>
    <name>Q67KK4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196928.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2809"/>
    <dbReference type="EnsemblBacteria" id="BAD41794">
        <property type="protein sequence ID" value="BAD41794"/>
        <property type="gene ID" value="STH2809"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2809"/>
    <dbReference type="HOGENOM" id="CLU_990196_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="29166" checksum="514504D075B791FF" modified="2004-10-11" version="1">MANVLERGIAVWRRHWPGLFLACLAVAVVPGLTVALVGALLGLLPVSLADALVLLLPVQFAGQGADVTLFFGQKGSWVLLVLYGVLVLLGSAGILGILWTVVHEGRAPKASDFLGGIRRYAGRLVGVRVLCGLVLVAAALAAGLVYVLAGSVLAGWLVPIAGLAALLAGISAVLYADFALVVEEGTATEAMGDSVGVLTERWREAVGAALMFILISVAGEAAGWLVARIIPGLPGLLLSLLPVALAESILLAYLAVRYVEHVHRGIYGRDGRFAIDERKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK5</accession>
    <name>Q67KK5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative muramoyltetrapeptide carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S66 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196927.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2808"/>
    <dbReference type="MEROPS" id="S66.001"/>
    <dbReference type="EnsemblBacteria" id="BAD41793">
        <property type="protein sequence ID" value="BAD41793"/>
        <property type="gene ID" value="STH2808"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2808"/>
    <dbReference type="eggNOG" id="COG1619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034346_3_1_9"/>
    <dbReference type="OMA" id="MLTQWRL"/>
    <dbReference type="OrthoDB" id="952832at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027461">
        <property type="entry name" value="Carboxypeptidase_A_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027478">
        <property type="entry name" value="LdcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040449">
        <property type="entry name" value="Peptidase_S66_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040921">
        <property type="entry name" value="Peptidase_S66C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003507">
        <property type="entry name" value="S66_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237">
        <property type="entry name" value="PTHR30237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02016">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17676">
        <property type="entry name" value="Peptidase_S66C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028757">
        <property type="entry name" value="LD-carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141986">
        <property type="entry name" value="SSF141986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S66" evidence="4">
        <location>
            <begin position="18"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S66C" evidence="5">
        <location>
            <begin position="186"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR028757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="33999" checksum="E0DA1942A2CB8967" modified="2004-10-11" version="1">MTGAAVRRPRPVPLGGTVGVVAPAGPVPPEALEEGLRRLRAWGYRTVVGEAVLDRRGYLAGADDRRAEDFNRMWADPAVDAVICARGGYGAMRILRRIDWELIRRNPKFFCGFSDITALHLAMAREAGLVTFHGPMVAAFGGAHGYNGAGLRRALQQVGPLGEMPWPDPAEEGAPRPLTIRSGVAEGRLIGGNLTLIVSLLGTPWEPDFTGCIVLLEDVDEAPYRVDRMLVQLLLAGKLQGAAGILFGDSPTCMHGPPDRPSLTLPEVLTELLGPLGIPVLYGFPCGHGPHRATLPLGVRTRLDAAGGTLTILEPALEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR6</accession>
    <name>Q67KR6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2746"/>
    <dbReference type="EnsemblBacteria" id="BAD41731">
        <property type="protein sequence ID" value="BAD41731"/>
        <property type="gene ID" value="STH2746"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2746"/>
    <dbReference type="HOGENOM" id="CLU_1085545_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="26854" checksum="22CC9B3F1EE671CF" modified="2004-10-11" version="1">MCTPALVWPPIQRAAGNPDSDVPAAPVPAQNLNSPPTPDPRFCAPATSPPPAHAQNLNLPPTPNHRFCAPAPCSPPVHAQNLNQPSTPDFRFCAPAPCSPPAHAQNLNLPPTPNHRLCAPATSPPPAHAQNLNSLPTPDLRFCAPTPCSPPVHAQNLNSLPTPDLRFCAPATCSPPVHAQNLNSPPTPDLRFCAPAPCPSHAHAQNLNSPPTPDLRFCAPATCPPPVHAQNLNSPPTPDPRFCAPAPCPPPLTRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT1</accession>
    <name>Q67KT1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-N-acetylmuramyl-tripeptide synthetase</fullName>
            <ecNumber evidence="8">6.3.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UDP-MurNAc-tripeptide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">murE</name>
        <name evidence="13" type="ordered locus">STH2731</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.-" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2731"/>
    <dbReference type="EnsemblBacteria" id="BAD41716">
        <property type="protein sequence ID" value="BAD41716"/>
        <property type="gene ID" value="STH2731"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2731"/>
    <dbReference type="eggNOG" id="COG0769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022291_4_1_9"/>
    <dbReference type="OMA" id="FIPGRML"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 8 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="12">
        <location>
            <begin position="103"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="11">
        <location>
            <begin position="329"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-MurNAc-L-Ala-D-Glu binding" evidence="8">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="105"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="8">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD41716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="496" mass="53238" checksum="522C3872CD100988" modified="2004-10-11" version="1">MLSALDPVQVLEAPDVTVTGIAADSRAVRPGDLFVCIPGAHVDAHQFIPDALRRGAVAVVTQRPWAVPTGIGAAVVPDARLALSALADRFYGHPSRALRMVGVTGTNGKTTTAFLVEAIARTAGKAMGVIGTAGMMLGREVLEGKSGYTTPEPQTIHRLLAAMLQRGAWGAVMEVSSHALEQRRTDHCRFDVAVFTNLTHEHLDYHGDMEGYYAAKARLFRGLQPGAAAVVNIDDPYGRRLAEEIPAGVRTLTYGFSEGADIRAEDVHLTGPGARYRLITPVGEADVEAPHLFGTYNVSNAMAAVGAGLALGFSLQDAVAALGTAKGAPGRFERIDEGQDFTVVVDYAHTPDGFEKLLSDVARIKEPGSRVIMVFGSAGQRDPSKRPDMGRIAGEYCDVLVLTEEDPRTEDAMEIARQIASGVARDEVEIHLIEDRVEAIREAIRMARTGDIVLITGKGQETELEVQHPTTWRGDVVEAVDALRDRAREEARGVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX3</accession>
    <name>Q67KX3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ketol-acid reductoisomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2688</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2688"/>
    <dbReference type="EnsemblBacteria" id="BAD41673">
        <property type="protein sequence ID" value="BAD41673"/>
        <property type="gene ID" value="STH2688"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2688"/>
    <dbReference type="eggNOG" id="COG0059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_2_9"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="7">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="8">
        <location>
            <begin position="120"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="30203" checksum="4ED48CAD590B4F47" modified="2004-10-11" version="1">MAPVAAAAEMADVIMLLINDEHQGRVFAEQIRPHLKPGKALGFGHGFAVHFGQVRPPADVDVFMIAPKGPGHLVRRQYEEGRGVPCLMAIHQDATGRCRDIALAWAKGIGGTRAGVIETTFREECESDLFGEQAVLCGGLTQLVKYGFEVLTEAGYAPEIAYFECLHELKLIVDLMYEGGMAAMRYSISDTAEYGDYVSGPRVIGPEVKERMKQILAEIQNGTFAKDWILENQVGRPRFTALRRQNAAHPVEQVGARLRAMMPWLPKPVGAVGNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX6</accession>
    <name>ILVD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="1">DAD</shortName>
            <ecNumber evidence="1">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvD</name>
        <name type="ordered locus">STH2685</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196807.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KX6"/>
    <dbReference type="SMR" id="Q67KX6"/>
    <dbReference type="STRING" id="292459.STH2685"/>
    <dbReference type="EnsemblBacteria" id="BAD41670">
        <property type="protein sequence ID" value="BAD41670"/>
        <property type="gene ID" value="STH2685"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2685"/>
    <dbReference type="eggNOG" id="COG0129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_9"/>
    <dbReference type="OMA" id="TQGRNMA"/>
    <dbReference type="OrthoDB" id="193579at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000225430" description="Dihydroxy-acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <sequence length="565" mass="59431" checksum="F5E5B2479EC6E4F3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ1</accession>
    <name>Q67KZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Snf2 family helicase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2670"/>
    <dbReference type="EnsemblBacteria" id="BAD41655">
        <property type="protein sequence ID" value="BAD41655"/>
        <property type="gene ID" value="STH2670"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2670"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_11_9"/>
    <dbReference type="OMA" id="YVWEQCD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="63"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="325"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="499" mass="56229" checksum="853A30C01273F343" modified="2004-10-11" version="1">MTHSLGLPPAPSLFAGDGWRAQSPDAPLPFFDLPAPPEGAAPDPDALYAPPGEELYDYQVDGARFLMEHPVALLGDEMGLGKSIQAISALRLLIRRGEVGRALILCPKTLIFDWYYKLRLWAPDLRVVPVEGPRRRRQWYWRCQVHVHLVGYESWREDLKAGLASPDVYDLIVLDEVQRIKNPGTAIHKAVAQVRAPWRWGLSGTPLENRVEELLAIFAYLKPGLLPGRKGCPVEEIHRRIAPYVLRRRKADVLRHLPPKEHRTVWLDLTPAQRMAYEAAERAAVAAIRQAGHAAAPVIALTLLNQLKQLCNLDPATGASCKLAFLEQELAPLVARGEKAVIFSQYPKVTLEPLLPRLEPFGAVLFDGSLSDWKRQLIVHHFQHGDMPRVLAMSLKAGGVGITLTRANHVYHLDHWWNPAVAQQAEDRTHRIGQRRPVFVTTLLTRGTVEERIAELVERKRELFHEVMDPLTEPGEPEEQPYLLQRLSRAEILSLFGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L17</accession>
    <name>Q67L17_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DinB_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2644"/>
    <dbReference type="EnsemblBacteria" id="BAD41629">
        <property type="protein sequence ID" value="BAD41629"/>
        <property type="gene ID" value="STH2644"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2644"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107587_1_0_9"/>
    <dbReference type="OMA" id="VWQQYMN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="1">
        <location>
            <begin position="25"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="18345" checksum="25D2F4640F1F8BBD" modified="2004-10-11" version="1">MQGRVELLGRMREFAWNREGWFVPLEEALRGLTAREAAWRPLVGGLTIWQILNHLNYWNTYMLRRITGVSPDTPVLENEETFGSQGDPNDEDGWHALVAQTRQIAEELRAALADLSESDLDRPLPNMGTVADSLAAWVMHDAYHTGQIILIRKLQGAWPPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L40</accession>
    <name>Q67L40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2621</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196743.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2621"/>
    <dbReference type="EnsemblBacteria" id="BAD41606">
        <property type="protein sequence ID" value="BAD41606"/>
        <property type="gene ID" value="STH2621"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2621"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="RCIPFLE"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="240" mass="26467" checksum="4FA685BC70C30F43" modified="2004-10-11" version="1">MIRVRNLRKSFGRLEVLRGIDLDVARGEVVALIGPSGSGKSTLLRCVNLLEQPTAGSIAVDGQEITAPGFDVNRLRQRVGMVFQHFNLFPHMTVLRNVTLAPVLTGRLSAKDAEERALELLGRVGLRDKAHAYPDSLSGGQKQRVAIARALAMNPEVMLFDEPTSALDPEMVKEVLEVMKQLALEGMTMLVVTHEMGFAREVCDRVIFMDEGRLVEQGEPQQVFSSPQHPRTAAFLKAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L41</accession>
    <name>Q67L41_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41605">
        <property type="protein sequence ID" value="BAD41605"/>
        <property type="gene ID" value="STH2620"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2620"/>
    <dbReference type="HOGENOM" id="CLU_2810899_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="67" mass="7001" checksum="A74ED9B4DCC0A1B4" modified="2004-10-11" version="1">MMAEILILLGLLSIVLLRPTSVEIRRRELQHGYDVFGSALLAAPGNPGREPSGPHRTPAGTAGPSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L50</accession>
    <name>Q67L50_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196733.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2611"/>
    <dbReference type="EnsemblBacteria" id="BAD41596">
        <property type="protein sequence ID" value="BAD41596"/>
        <property type="gene ID" value="STH2611"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2611"/>
    <dbReference type="eggNOG" id="COG4586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1359816_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="22555" checksum="E531BC6AE7813F33" modified="2004-10-11" version="1">MTASTVIWRFRTATEPWTSDRSLAELAALTLAQGGRAALRTWTGSGGDSHSGDGTAAVPDGVAYVHGERHDLWWEFTLEASFRWQAHLCRLRPDVYRQRLRRVTRALGLEEVLQREVASLTHGTRALADLAVALMQKPRLLLWEEPFYYMGRDEALRAVRLVEAEHLAGMTVVAVAREAPGLADLPAEPRRPHLRRLRAAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-02" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC0</accession>
    <name>FTSH1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent zinc metalloprotease FtsH 1</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsH1</name>
        <name type="ordered locus">STH2541</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LC0"/>
    <dbReference type="SMR" id="Q67LC0"/>
    <dbReference type="STRING" id="292459.STH2541"/>
    <dbReference type="PRIDE" id="Q67LC0"/>
    <dbReference type="EnsemblBacteria" id="BAD41526">
        <property type="protein sequence ID" value="BAD41526"/>
        <property type="gene ID" value="STH2541"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2541"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000400401" description="ATP-dependent zinc metalloprotease FtsH 1">
        <location>
            <begin position="1"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="2"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="114"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="186"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <sequence length="594" mass="64245" checksum="4B68F253E4D3BB83" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE0</accession>
    <name>Q67LE0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nitro_FeMo-Co domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2521"/>
    <dbReference type="EnsemblBacteria" id="BAD41506">
        <property type="protein sequence ID" value="BAD41506"/>
        <property type="gene ID" value="STH2521"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2521"/>
    <dbReference type="eggNOG" id="COG1433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_2_2_9"/>
    <dbReference type="OMA" id="CQNMPNP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="10"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="117" mass="12552" checksum="1E7BF8B1B962120B" modified="2004-10-11" version="1">MMRIAIPLADGDVAPHVGHCRSYLIADVEDGKVVHTEEVANPGHGPGGPPPVFLANQGVNLVLAWGIPQHARELFRRHGVEYIVGCHGEARRVLQDYLAGTLKLTDQGLEGDGCSCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ5</accession>
    <name>Q67LJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methylmalonyl-CoA mutase N-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2466"/>
    <dbReference type="EnsemblBacteria" id="BAD41451">
        <property type="protein sequence ID" value="BAD41451"/>
        <property type="gene ID" value="STH2466"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2466"/>
    <dbReference type="eggNOG" id="COG1884">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009523_5_1_9"/>
    <dbReference type="OMA" id="PWGGSAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004494">
        <property type="term" value="F:methylmalonyl-CoA mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006099">
        <property type="entry name" value="MeMalonylCoA_mutase_a/b_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006098">
        <property type="entry name" value="MMCoA_mutase_a_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01642">
        <property type="entry name" value="MM_CoA_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00641">
        <property type="entry name" value="acid_CoA_mut_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="METMALONYL_COA_MUTASE" evidence="1">
        <location>
            <begin position="14"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="534" mass="60056" checksum="2128EBCE1CDC8ECE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM1</accession>
    <name>Q67LM1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Magnesium chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196563.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2440"/>
    <dbReference type="EnsemblBacteria" id="BAD41425">
        <property type="protein sequence ID" value="BAD41425"/>
        <property type="gene ID" value="STH2440"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2440"/>
    <dbReference type="eggNOG" id="COG1240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015279_0_0_9"/>
    <dbReference type="OMA" id="CLIIDAS"/>
    <dbReference type="OrthoDB" id="247788at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="563"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="741" mass="77993" checksum="5F97B3455A92C83E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ7</accession>
    <name>Q67LZ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2314</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2314"/>
    <dbReference type="EnsemblBacteria" id="BAD41299">
        <property type="protein sequence ID" value="BAD41299"/>
        <property type="gene ID" value="STH2314"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2314"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_9"/>
    <dbReference type="OMA" id="PDFWMGI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="77"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="34677" checksum="D6F0D0D4796E1FC2" modified="2004-10-11" version="1">MLVVIFVLMRAIPGDPARLMLGERASPEAVAALREQMGLNDPLYVQFFTFLGDVLRGDLGVSLQSRQPVTAELMARYPATVELAIFAMLLASVLGILAGVVSAVWRNSLLDYGVMAAALVGVSMPIFWLGLVLMMLFSVRLHWLPAGGRLDARMIWDGATQFVVLESLVRGHWTVFVTGLRHLILPGIALATIPLSIIARITRSSMLEVLNLDYIRTARAKGLPNWRVNFRHALKNALLPVVTIIGLQVGLLLGGAVLTETIFSWPGIGRYIVMAISARDYPVVQGGILLITLAMILVNLLVDVIYTLIDPRIRYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M33</accession>
    <name>Q67M33_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6431 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41262">
        <property type="protein sequence ID" value="BAD41262"/>
        <property type="gene ID" value="STH2277"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2277"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1805185_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045536">
        <property type="entry name" value="DUF6431"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20020">
        <property type="entry name" value="DUF6431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6431" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="143" mass="16933" checksum="07680991026C74A2" modified="2004-10-11" version="1">MCILLPLGQNVQTYLRKYGTCGPDLPLHCPGCGDRMRRHGRYWRWVFTAHQKAYIPIYRWWCPGCRKTCAILPDFLKPYARFITLVREAVVRGRVRRGLPWSTLARRLSSPTVSWLSEKRGCRETHAMKRESLREQEPRKRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M65</accession>
    <name>Q67M65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41230">
        <property type="protein sequence ID" value="BAD41230"/>
        <property type="gene ID" value="STH2245"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2245"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="2">
        <location>
            <begin position="299"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="548" mass="61425" checksum="08CD9E3D6F36C172" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH4</accession>
    <name>Q67MH4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2134"/>
    <dbReference type="EnsemblBacteria" id="BAD41119">
        <property type="protein sequence ID" value="BAD41119"/>
        <property type="gene ID" value="STH2134"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2134"/>
    <dbReference type="HOGENOM" id="CLU_1446971_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="187" mass="20110" checksum="00633D272B9C2FB3" modified="2004-10-11" version="1">MGSPAEGWPLPDAHDGIDLFRGGQLLRNAAEAYGRALTPEEYWWLGVGLAAATLAELGGREYAARRIQQLLGRLGGPGDPEEWFEQFHNELAALDAEGLLWPLYQRDERGKMARTDAVVAAGFDADAGWALALAHLAIARAVLEERQEAIPTLAEVIADLIEGAPAAEHLPRVQAALGMQPEGAEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI8</accession>
    <name>Q67MI8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative sodium/calcium exchanger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2120"/>
    <dbReference type="EnsemblBacteria" id="BAD41105">
        <property type="protein sequence ID" value="BAD41105"/>
        <property type="gene ID" value="STH2120"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2120"/>
    <dbReference type="eggNOG" id="COG0530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007948_2_1_9"/>
    <dbReference type="OMA" id="NIFNMVI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="PTHR10846"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="164"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="32768" checksum="6B3820B8203DC4A3" modified="2004-10-11" version="1">MIIVAGRAISNASDELAERTGLGRAFIGSLLLAGATSLPEVAASGSAAFMGSGNLALGNVFGSNIFNMILLVVGQIFATRHILSNVSPTHITTAATGMLLSGIAALSILAPQPYAFLGAGLDTWLIAVLYIVIMRMLPGAEQEPEVAAATQARDAEPTTSLTVLWLKFAASAAAILVAGWFLSKAADEIAVITGLGQTFIGSTLLAASTSLPELTVSIFTARMGAYDLLVGNVLGSNIFNMVILIVSDLLQPGSTPILSTGTTGQAVSALVGLVLSGIVIVSLTLPRREGKFKFSWDMWLILAVYLVGLRMIYQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML6</accession>
    <name>Q67ML6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196217.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2092"/>
    <dbReference type="EnsemblBacteria" id="BAD41077">
        <property type="protein sequence ID" value="BAD41077"/>
        <property type="gene ID" value="STH2092"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2092"/>
    <dbReference type="eggNOG" id="COG2322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104065_2_0_9"/>
    <dbReference type="OMA" id="HRKIARW"/>
    <dbReference type="OrthoDB" id="1623896at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007352">
        <property type="entry name" value="DUF420"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37692">
        <property type="entry name" value="PTHR37692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04238">
        <property type="entry name" value="DUF420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="15914" checksum="3CC823E840C6238F" modified="2004-10-11" version="1">MDRILAVLPTVNAILISVSGILILWGIRLVRRGEPQRHQRVMLTATALAALFLVLYVTRVFLGGLSTFSGPGWLRPIYLAILISHFTLAMVQTPLVLITVWRGLRGLLPSHRRLGRVTWPIWVYVSATGVLVYGLLRFPYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML8</accession>
    <name>Q67ML8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF5668 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2090"/>
    <dbReference type="EnsemblBacteria" id="BAD41075">
        <property type="protein sequence ID" value="BAD41075"/>
        <property type="gene ID" value="STH2090"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2090"/>
    <dbReference type="eggNOG" id="COG4758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073800_0_0_9"/>
    <dbReference type="OMA" id="NMYGFLP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043726">
        <property type="entry name" value="DUF5668"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18917">
        <property type="entry name" value="DUF5668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5668" evidence="3">
        <location>
            <begin position="58"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="40517" checksum="702ABDF61F3C56B7" modified="2004-10-11" version="1">MHSDAGGPPPGTPGGRGPAAFARDEGGRDEEGTSGPRGPHGPLYHPPPPPPRSRRQSIVGPLVLIFLGLFFLGQSLGLIEWSLWEVVWRLWPVWLIVAGLDMVFGQRGGWARALLVLIAVAIVLGIVLGIQPQRAIDRPTGAVPSSPSRTFLMEPSERVPAAPSLPVTGQQPEPVTIAQPLEGISAAEVRIESAVSVLEIRGGDLPDLLIEGTVVPLIGEQVQWDYDAVDGTGVFRLYSDKPTRISSGPRQGEWDLVLTDRIPISLHLSTGVADSDIDLTRLHVPELNVEASVGEVSIKLPETGAVKGTINAGIGEVILWIPEGRPARIRVETGIGSTSVAPGFLYRDGYYVTPQYADQEDAVDLVVTGGVGTVDLRIMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM2</accession>
    <name>Q67MM2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2086</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41071">
        <property type="protein sequence ID" value="BAD41071"/>
        <property type="gene ID" value="STH2086"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2086"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_12_2_9"/>
    <dbReference type="OMA" id="DETPAWM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="7">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="6">
        <location>
            <begin position="277"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="452" mass="52119" checksum="B43B36DB604C06F3" modified="2004-10-11" version="1">MSKKTYRPYEPNQLLLLPPALQDWLPHDHFAYFLSDVVDSLDLSQIEQTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMSYSRMVEEEQRLKAEIAELLQKAEEADQAEDHRFGPHRRGDELPEELRFRERRLAKIQEAKAALEAEARLRAGADEHEPPEEPGPKGPKGGRRRKRPKAEPAPTAQRNFTDPDSRIMKGSDKQFIQGYNAQAVVDADTQIVVAAAVTNQAADAPHLIPMMEQVETNVDEMPEEVSADAGYFSEQNVQWLLQRRINPYIPPEKQRHSERRRAKSPRGPIPKGATLRDRMRRKLQLKRHAERYRKREQSIEPVFGQIKEARGFRQFHLRGLEAVNGEWLLVCLCHNLLKLYAFSQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM3</accession>
    <name>Q67MM3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2085"/>
    <dbReference type="EnsemblBacteria" id="BAD41070">
        <property type="protein sequence ID" value="BAD41070"/>
        <property type="gene ID" value="STH2085"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2085"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132482_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007061">
        <property type="entry name" value="DUF664"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04978">
        <property type="entry name" value="DUF664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="209" mass="23298" checksum="35C3FAB47B59D186" modified="2004-10-11" version="1">MAVGRAQGLSAGAASWCKSTLGVWGRSDSGIVPLRRGGLAMRDVHSSGVPQALAVPFGLVEDAFRRLEKRLEGMSEEEFHFKAPDNVNSTAMLLRHLVLVDMTYLHLIMGDSERVPEIRGKYGPFQDENGRIPEAQGATLSGLLGEYRELMDTARRYISGLSDEDAERAVVVPWWPEPATVRYMLWHMAGHSCHHQGQIARLRAAYRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ6</accession>
    <name>Q67MQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2052"/>
    <dbReference type="EnsemblBacteria" id="BAD41037">
        <property type="protein sequence ID" value="BAD41037"/>
        <property type="gene ID" value="STH2052"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2052"/>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2669742_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="7854" checksum="B2B6FB051B1BD89D" modified="2004-10-11" version="1">MRLFGTLRLDAGRRRVEVDAATVAEALEKTAAALGLADPGPLLQGAVYVNGERAGMRTRLKDGDELYLLSPAAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU7</accession>
    <name>Q67MU7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196138.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2011"/>
    <dbReference type="EnsemblBacteria" id="BAD40996">
        <property type="protein sequence ID" value="BAD40996"/>
        <property type="gene ID" value="STH2011"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2011"/>
    <dbReference type="eggNOG" id="COG1321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_3_0_9"/>
    <dbReference type="OMA" id="SWDAIDR"/>
    <dbReference type="OrthoDB" id="2047422at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="17487" checksum="E2ED1E5FAB7B54C9" modified="2004-10-11" version="1">MATPSMEDYVEKIYELIQSKGYARVSDIAMELNLQPSSVTKMVQKLAEAGYIHYERYRGLVLTPAGQLLGEQMKDRHTMLSEFLRTIGVPEEVIEVDVEGIEHHVSPSTVRCIQDLTSFLQENEDVREALAAHRRRRREREAAEQAAREQAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV7</accession>
    <name>Q67MV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2001"/>
    <dbReference type="EnsemblBacteria" id="BAD40986">
        <property type="protein sequence ID" value="BAD40986"/>
        <property type="gene ID" value="STH2001"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2001"/>
    <dbReference type="eggNOG" id="COG4467">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1209290_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269553" evidence="2">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25920" checksum="792DF3C5045477DF" modified="2004-10-11" version="1">MRRASVATLLAATAAVFVACTSPPPPPGTSMEVEELSAQVAELTAVNQRLEAENRELEARVAELDAKVKELEFRNQNLADRLSPEEREVNLINPRFPPAVGGEPGWEYHQVLSADLDNDGVEERVSVTTNAFWMEDRKEFGWDDGHPWHVYVEEPDGTRTYLFSDWVQLGKLDVILDREGPGVFIVYRRDGGMIIYRATYQGPGQFRTVRSYQIPLSYSATWANPDMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW6</accession>
    <name>Q67MW6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endolytic murein transglycosylase</fullName>
            <ecNumber evidence="7">4.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peptidoglycan polymerization terminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mltG</name>
        <name evidence="8" type="ordered locus">STH1992</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transglycosylase MltG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196119.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1992"/>
    <dbReference type="EnsemblBacteria" id="BAD40977">
        <property type="protein sequence ID" value="BAD40977"/>
        <property type="gene ID" value="STH1992"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1992"/>
    <dbReference type="eggNOG" id="COG1559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025574_2_0_9"/>
    <dbReference type="OMA" id="IAMPGKA"/>
    <dbReference type="OrthoDB" id="708179at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08010">
        <property type="entry name" value="MltG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02065">
        <property type="entry name" value="MltG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003770">
        <property type="entry name" value="MLTG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30518">
        <property type="entry name" value="PTHR30518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02618">
        <property type="entry name" value="YceG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00247">
        <property type="entry name" value="TIGR00247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="38098" checksum="D4817ACAD7BD20E3" modified="2004-10-11" version="1">MSNAAKAVRLVLALLILLAALGGAAYGGLRWALARLEPADPTAGLLEVEIPSGATTTDVAQILAEHGIIRDPAVFRYYVRYRELDGQIVSGRYELSAAMSADQILTKLVNGEVVVRRFTIPEGLTVEMMADLLAEAQVVDREAFLDAALAAAAENPYLPEDVELIQPMEGYLFPATYQYHSGITAEEVVAMLMARFEAVWTPELLARADEMGLSVHEVTTLASIVETEARVAAEQPQIAGVYLNRLAVGMPLQADPTVYYALGLPRSEALSYDDLEVDSPYNTYRYPGLPPGPIAAPGEGAIRAVLYPATHDYYYFVAKNDGSGEHYFATTYAEHLENVDRAEANLAEQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY8</accession>
    <name>Q67MY8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196097.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1970"/>
    <dbReference type="EnsemblBacteria" id="BAD40955">
        <property type="protein sequence ID" value="BAD40955"/>
        <property type="gene ID" value="STH1970"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1970"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="QSIVTFP"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="28538" checksum="34AECEE0F916F8E1" modified="2004-10-11" version="1">MDRPRITLEEVSLTYLSPRGATQALAGVSLAVWPGEFVSIVGPSGCGKSTILSLIAGILRPTAGRVLLDGEPVSGPSRRVGYMLQRDYLFEWRTVTENCLIGPEVQRQDMARARDRVAGLLRKTGLSAFADAYPDQLSGGMRQRAALVRTLAIDPDILLLDEPFSALDFQTKLTLIDEVWSLLRSEQKTVLLVTHDISEAISMSDRVIVLSRRPGRVKSVHEIRFAGPERPIPFQARSAPEYGGYFHRIWAELDPPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N50</accession>
    <name>Q67N50_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Type I restriction enzyme endonuclease subunit</fullName>
            <shortName evidence="12">R protein</shortName>
            <ecNumber evidence="12">3.1.21.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Type-1 restriction enzyme R protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH1908</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Subunit R is required for both nuclease and ATPase activities, but not for modification.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1908"/>
    <dbReference type="REBASE" id="10270">
        <property type="entry name" value="StpORF1910P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40893">
        <property type="protein sequence ID" value="BAD40893"/>
        <property type="gene ID" value="STH1908"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1908"/>
    <dbReference type="eggNOG" id="COG0610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005762_1_0_9"/>
    <dbReference type="OMA" id="YDMTQAV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004473">
        <property type="entry name" value="Restrct_endonuc_typeI_HsdR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040980">
        <property type="entry name" value="SWI2_SNF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021810">
        <property type="entry name" value="T1RH-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11867">
        <property type="entry name" value="DUF3387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18766">
        <property type="entry name" value="SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00348">
        <property type="entry name" value="hsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="265"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1063" mass="119940" checksum="03134D5B6D93062D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N82</accession>
    <name>Q67N82_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA thioester hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196003.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1876"/>
    <dbReference type="EnsemblBacteria" id="BAD40861">
        <property type="protein sequence ID" value="BAD40861"/>
        <property type="gene ID" value="STH1876"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1876"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_3_2_9"/>
    <dbReference type="OMA" id="KWIDQAG"/>
    <dbReference type="OrthoDB" id="1712258at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="18395" checksum="D6E3510ED2BAB6D3" modified="2004-10-11" version="1">MHTPEQHLEPKPARLSACEMTEIVMPNDANPLGIMLGGRLLHIMDIAATIAAMRHAGHPCVTTSFDSVDFHTPIHIGEVCIVRARVTWTGRTSLEVGIDVFAESPIKGERRHTTSAFATFVALDQETGRPTRVPPLLLETDEDRRCFAEGEQRRQLRLARRHARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE3</accession>
    <name>Q67NE3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH1815</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1815"/>
    <dbReference type="MEROPS" id="S11.005"/>
    <dbReference type="EnsemblBacteria" id="BAD40800">
        <property type="protein sequence ID" value="BAD40800"/>
        <property type="gene ID" value="STH1815"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1815"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_7_3_9"/>
    <dbReference type="OMA" id="TNEAKYC"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBP5_C" evidence="15">
        <location>
            <begin position="295"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="12">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD40800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="403" mass="43216" checksum="5A92E014291151C9" modified="2004-10-11" version="1">MERWPPAPDVEDGDRLLQGGGAYGLDEPVEAAAAAEEAGSGEPVKIESPSAILMEPLSGRVIYQKNAHERRNPASVTKIMTLIVAFDAIRDGKVSLKDPVTISEEAKRQPGTTIFADVGETFSLEELLLSVAVGSANDAAVAVAEHVAGSVDAFVALMNKKAKELGMNDTQFMNPTGLSAEGHYSTAYDIALMSRHAVLHYPELVKLTAIYGAELNVPWRKNGPKFQLWNNNKLLTWYPGADGLKTGWTQAAGYCLAATAVQNNTRMIAVVMGAESAKQRNIEVARLLDYGFANYTAVEIAPADKKFGPVRVARGAVAAIEPVPRAAFGVSVPKGSQDKVKWEVRLPKQVAAPLEKGQAVGEIVATLDGQEVGRMELVAPEAVGKANLWQSVVHTVRNIFSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF9</accession>
    <name>Q67NF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1799"/>
    <dbReference type="EnsemblBacteria" id="BAD40784">
        <property type="protein sequence ID" value="BAD40784"/>
        <property type="gene ID" value="STH1799"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1799"/>
    <dbReference type="eggNOG" id="COG3874">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2048535_0_0_9"/>
    <dbReference type="OMA" id="KFVNGRQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014229">
        <property type="entry name" value="Spore_YtfJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39162">
        <property type="entry name" value="PTHR39162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09579">
        <property type="entry name" value="Spore_YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021377">
        <property type="entry name" value="YtfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02874">
        <property type="entry name" value="spore_ytfJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="120" mass="12400" checksum="085FBCF0A94242B5" modified="2004-10-11" version="1">METQDGTVIVPVSRVTFGFAAGGSEFVPAHEREPGHDHPFGGGSGAGVSVSPVGFLVVSKDQVRMLPVDGGQPVHRIIDLAPQLIDKLRAALGGRAQDEPCEPEVSDTTGADGTPKTTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ4</accession>
    <name>Q67NJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1764"/>
    <dbReference type="EnsemblBacteria" id="BAD40749">
        <property type="protein sequence ID" value="BAD40749"/>
        <property type="gene ID" value="STH1764"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1764"/>
    <dbReference type="HOGENOM" id="CLU_1905708_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="133" mass="13785" checksum="391F1A81974DBAA4" modified="2004-10-11" version="1">MTVVITVAQIGLPVWCTGGGRCMRCGACGREVDGGPGDLCPVCGGVLGADYVTATPESSLAGFGKVVRGIRPARRRSRVVRAGALAILLPFAAASVAWGYQVWQNAGGRLTLGMAMPALLLLLTGAVAFRWLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK1</accession>
    <name>MUTS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutS</name>
        <name type="ordered locus">STH1757</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NK1"/>
    <dbReference type="SMR" id="Q67NK1"/>
    <dbReference type="STRING" id="292459.STH1757"/>
    <dbReference type="EnsemblBacteria" id="BAD40742">
        <property type="protein sequence ID" value="BAD40742"/>
        <property type="gene ID" value="STH1757"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1757"/>
    <dbReference type="eggNOG" id="COG0249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_1_3_9"/>
    <dbReference type="OMA" id="TPMMAQY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224413" description="DNA mismatch repair protein MutS">
        <location>
            <begin position="1"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="625"/>
            <end position="632"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <sequence length="875" mass="96685" checksum="F1B62AEB01499E98" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK6</accession>
    <name>Q67NK6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Small heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195880.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1752"/>
    <dbReference type="EnsemblBacteria" id="BAD40737">
        <property type="protein sequence ID" value="BAD40737"/>
        <property type="gene ID" value="STH1752"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1752"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_0_9"/>
    <dbReference type="OMA" id="NMRDIIP"/>
    <dbReference type="OrthoDB" id="1523369at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="34"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="150" mass="16924" checksum="F616EA38DB4B39AB" modified="2004-10-11" version="1">MDLIPWNPLRELERIRTPLERFFADPFLGPTMPFSGGMGAFPVEVAEQGDDIVVRCELAGIDPQDVDVRITDQGVTIRGERKAEEVDRQRGTYRSERFYGAFSRTVSFPVPVDSERAQATFRHGLLEVRAPKRNPDAGPDGRRLNITPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR8</accession>
    <name>Q67NR8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amino acid amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1690"/>
    <dbReference type="MEROPS" id="M20.020"/>
    <dbReference type="EnsemblBacteria" id="BAD40675">
        <property type="protein sequence ID" value="BAD40675"/>
        <property type="gene ID" value="STH1690"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1690"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_2_1_9"/>
    <dbReference type="OMA" id="YFTAIHI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="237"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="446" mass="46942" checksum="062E4FF1FB18F283" modified="2004-10-11" version="1">MKEVLPVDISTRVQQLAPDLVRVRRDLHAHAESGWTEFRTAALVVQRLSALGYEVRYGPEVLSPDHMAGLPPREELDRHLERAVAQGADPEVVRRMAGGFTAVVGVLRCGEGPTVALRFDMDALDVTETDDPGHRPNREGFASRNPGAMHACGHDGHTAIGLGVAAVLADLKDELRGTVKLLFQPAEEGVRGARAMVEAGVCDDVDYLLGAHLGFAATEVGTVIAGAGGFLATSKLDAVFTGVPSHAGAAPEAGRNALLAAAAAALNLHAIARHSKGASRINVGVLQAGSGRNVIPARAVMKLETRGETTAIDAYMREEAERIIRAAAAMHGVQVEILPIGSAAGGASDPDLAEVVARAAAETPSVTRILPAGDLGGSEDFAWMMDRVQRRGGKAVYMMIGTQIAAPHHDSRFDFDERALPIGVEVMARAVLRLAGGGIPQNSRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU4</accession>
    <name>Q67NU4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="1">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="1">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1664"/>
    <dbReference type="EnsemblBacteria" id="BAD40649">
        <property type="protein sequence ID" value="BAD40649"/>
        <property type="gene ID" value="STH1664"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1664"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_2_9"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="HXTX 1" evidence="1">
        <location>
            <begin position="45"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="1">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="21004" checksum="89E063C5595509E5" modified="2004-10-11" version="1">MRCFAAVEVSSPDVRQALVRACDELRRALGADAGLVKWVRPDQFHFTLRFFGELGPTEVEAAAQALRRAAAGTAPFPLAVAGLGAFPSARSPRVLWAGTGEGREQLIALARRLEDELAAAGFGREERPFQPHLTLGRVRQGADLAAPLRGLLTRPPVRYGDWLVDRLVLMRSELLPAGPRYTVVEAAELVRGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW5</accession>
    <name>CINA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative competence-damage inducible protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cinA</name>
        <name type="ordered locus">STH1643</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CinA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195772.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NW5"/>
    <dbReference type="SMR" id="Q67NW5"/>
    <dbReference type="STRING" id="292459.STH1643"/>
    <dbReference type="EnsemblBacteria" id="BAD40628">
        <property type="protein sequence ID" value="BAD40628"/>
        <property type="gene ID" value="STH1643"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1643"/>
    <dbReference type="eggNOG" id="COG1058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_9_3_9"/>
    <dbReference type="OMA" id="PGVPYEM"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00226_B">
        <property type="entry name" value="CinA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041424">
        <property type="entry name" value="CinA_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008135">
        <property type="entry name" value="Competence-induced_CinA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18146">
        <property type="entry name" value="CinA_KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006728">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00200">
        <property type="entry name" value="cinA_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156781" description="Putative competence-damage inducible protein">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00226"/>
        </source>
    </evidence>
    <sequence length="426" mass="45324" checksum="96ECBF4D821ADD31" modified="2004-10-11" version="1">MIAELVFVGTELLLGEILNTNAQYLSRQLAQLGVDVYHQVVVGDNAARLRAVLSQALSRSDLVIASGGLGPTDDDITREVAAEVTGRPLELDPQLLAQLEIWFARRGRRMAENNRRQCMVPRGARVLPNDRGTAPGLMIPADGDKVVILLPGPPGELRPMFEAHVAPYLAARSGGRPLRLVTRTLRFVGIGESALADGLRDLMATQTDPTIAPYAKVAEVHLRLATRAADEAEGYARIAPLEAEIRSRFGRFLYGSDEETLPQAVGRLLAERGMTLSTAESCTGGLVAKWITDVPGSSRYFGTGFVTYANEAKVSLLGVPEELLSAHGAVSEPVARAMAEGALQRSGADVAVAVSGIAGPDGGTPEKPVGTVCFALAGRGRQGGGPLPAGTWAETLWLHGDRDGVRERAAVHALAMVRRYLLGYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW9</accession>
    <name>Q67NW9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1639"/>
    <dbReference type="EnsemblBacteria" id="BAD40624">
        <property type="protein sequence ID" value="BAD40624"/>
        <property type="gene ID" value="STH1639"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1639"/>
    <dbReference type="HOGENOM" id="CLU_1128603_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="26379" checksum="2BDFDDA8278FBAC7" modified="2004-10-11" version="1">MRRLARSFRSAGGPQQNPAGSQAPAQQPGGPQDAPPQGRDQAYLEPQGAQQPPWLPSLNGETAASMLTALRAAKQQLVQELEANLKQLKAVLEETHRIQKQMEAVLMEARAEIGLQQRAGGHGQDQQPQRQQQPQARQPQAWGQMQQPQQLQQPQAQGQPPAQGHLQQPQGHGEPQPPQAPGHGRPQQPQAPSAGATGGPRGAQPPSPGAPGPRRPRPPLSSDPPPPWEPPEPASEPGWSPWQPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P02</accession>
    <name>Q67P02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1606</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195735.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1606"/>
    <dbReference type="EnsemblBacteria" id="BAD40591">
        <property type="protein sequence ID" value="BAD40591"/>
        <property type="gene ID" value="STH1606"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1606"/>
    <dbReference type="HOGENOM" id="CLU_2235207_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="105" mass="11713" checksum="1872A51F159847EE" modified="2004-10-11" version="1">MSTLSVRVRNPFLLRGSLEVVLEVARMDLANAEIEEIRGLLAAIPNSVRPTELQVPVAAARAALLAVRYFNQSRTRHWLREEMVNALLDLERALERHLRDAAGGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P14</accession>
    <name>NUOI1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit I 1</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit I 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit I 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoI1</name>
        <name type="ordered locus">STH1594</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195723.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P14"/>
    <dbReference type="SMR" id="Q67P14"/>
    <dbReference type="STRING" id="292459.STH1594"/>
    <dbReference type="EnsemblBacteria" id="BAD40579">
        <property type="protein sequence ID" value="BAD40579"/>
        <property type="gene ID" value="STH1594"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1594"/>
    <dbReference type="eggNOG" id="COG1143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067218_4_3_9"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="PTHR10849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000245752" description="NADH-quinone oxidoreductase subunit I 1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="1">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="1">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01351"/>
        </source>
    </evidence>
    <sequence length="162" mass="18288" checksum="53083EADEE908F9F" modified="2004-10-11" version="1">MNGVAAIAKGMATTLKVLFRKPVTVDYPYVKRPRAPRFRGRHELRTYENGLEMCVGCELCQVACPAAAITVQAAENDPDNPHSPGERYGYKYQVDLLRCIFCGMCEEACPTDCLHLTQEFELADFTRESLILQKEQLVNRNPSGFKVPMNVYPPFRRKAVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P49</accession>
    <name>Q67P49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195688.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1559"/>
    <dbReference type="EnsemblBacteria" id="BAD40544">
        <property type="protein sequence ID" value="BAD40544"/>
        <property type="gene ID" value="STH1559"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1559"/>
    <dbReference type="HOGENOM" id="CLU_189760_0_0_9"/>
    <dbReference type="OrthoDB" id="2084435at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025619">
        <property type="entry name" value="YlzJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14035">
        <property type="entry name" value="YlzJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8156" checksum="AE6A63DE2FC87128" modified="2004-10-11" version="1">MTECGPVVLWSVVPVEVILAGAEAGVPPLREVRVDGRLVLVAAGADGRGTVVRLISGQAQDYLDARFQPGATVMLERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P57</accession>
    <name>Q67P57_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195680.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40536">
        <property type="protein sequence ID" value="BAD40536"/>
        <property type="gene ID" value="STH1551"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1551"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="MQEETMA"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49913" checksum="74AF68B46DE156B5" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGEDGVPTERLRDQIRAAWERIREELLRGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRRLNAYLRGWGGYYALSDARSAFERLEGWLKRRLRACVWKQWKRVRTRFRELRALGLPEWVVHQLANSRKGPWRMAGGPLNSALGNAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD0</accession>
    <name>Q67PD0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0033 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1478"/>
    <dbReference type="EnsemblBacteria" id="BAD40463">
        <property type="protein sequence ID" value="BAD40463"/>
        <property type="gene ID" value="STH1478"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1478"/>
    <dbReference type="eggNOG" id="COG0425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_0_0_9"/>
    <dbReference type="OMA" id="VCPYPQL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="16"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="87" mass="9896" checksum="A110020307431ABB" modified="2004-10-11" version="1">MSTTGQGTLPQPDRAIDLRGDVCPVTFAKTKIALEEMQIGQVLLVRLDYEPATRNVPRSAELYGDEVLAVRQVGEREWEVWLRKRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE8</accession>
    <name>Q67PE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195590.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1460"/>
    <dbReference type="EnsemblBacteria" id="BAD40445">
        <property type="protein sequence ID" value="BAD40445"/>
        <property type="gene ID" value="STH1460"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1460"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094015_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="181" mass="20611" checksum="35C67A36914E4CB3" modified="2004-10-11" version="1">MHAVVTVLQPELYHRVEALWDDLERRFGLSGARITPIPHFSWQIAPEYDFARVEDCLQTVAQGLAPFEVETTGIGVFRGPAPVVFIDVRRTPALDALHVRLWEALRPHRRRESPYYSPEAWRPHITLAEKDLSPAHVERVVSHLSRLPLRWTVAVDNLALIEQPQGAVGRLRFSRRFGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI3</accession>
    <name>Q67PI3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Meso-diaminopimelate D-dehydrogenase</fullName>
            <shortName evidence="10">DAPDH</shortName>
            <shortName evidence="10">Meso-DAP dehydrogenase</shortName>
            <ecNumber evidence="5 10">1.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH1425</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <reference evidence="16 17 18" key="2">
        <citation type="journal article" date="2014" name="ChemBioChem" volume="15" first="217" last="222">
            <title>Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum.</title>
            <authorList>
                <person name="Liu W."/>
                <person name="Li Z."/>
                <person name="Huang C.H."/>
                <person name="Guo R.T."/>
                <person name="Zhao L."/>
                <person name="Zhang D."/>
                <person name="Chen X."/>
                <person name="Wu Q."/>
                <person name="Zhu D."/>
            </authorList>
            <dbReference type="PubMed" id="24339368"/>
            <dbReference type="DOI" id="10.1002/cbic.201300691"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS) IN COMPLEX WITH NADP</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible NADPH-dependent reductive amination of L-2-amino-6-oxopimelate, the acyclic form of L-tetrahydrodipicolinate, to generate the meso compound, D,L-2,6-diaminopimelate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>H2O + meso-2,6-diaminoheptanedioate + NADP(+) = (S)-2-amino-6-oxoheptanedioate + H(+) + NADPH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13561"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58556"/>
            <dbReference type="EC" id="1.4.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the diaminopimelate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.16" evidence="5 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195555.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="PDB" id="3WB9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.93 A"/>
        <property type="chains" value="A/B/C=1-299"/>
    </dbReference>
    <dbReference type="PDB" id="3WBB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.93 A"/>
        <property type="chains" value="A/B/C=1-299"/>
    </dbReference>
    <dbReference type="PDB" id="3WBF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.12 A"/>
        <property type="chains" value="A/B/C=1-299"/>
    </dbReference>
    <dbReference type="PDBsum" id="3WB9"/>
    <dbReference type="PDBsum" id="3WBB"/>
    <dbReference type="PDBsum" id="3WBF"/>
    <dbReference type="SMR" id="Q67PI3"/>
    <dbReference type="STRING" id="292459.STH1425"/>
    <dbReference type="EnsemblBacteria" id="BAD40410">
        <property type="protein sequence ID" value="BAD40410"/>
        <property type="gene ID" value="STH1425"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1425"/>
    <dbReference type="eggNOG" id="COG0673">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055796_1_0_9"/>
    <dbReference type="OMA" id="WDPGLFS"/>
    <dbReference type="OrthoDB" id="803114at2"/>
    <dbReference type="BRENDA" id="1.4.1.16">
        <property type="organism ID" value="6177"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00026"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047850">
        <property type="term" value="F:diaminopimelate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010190">
        <property type="entry name" value="Diaminopimelate_DH_Ddh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032094">
        <property type="entry name" value="Meso-DAP_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16654">
        <property type="entry name" value="DAPDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025648">
        <property type="entry name" value="DDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01921">
        <property type="entry name" value="DAP-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="16 17 18" id="KW-0002">3D-structure</keyword>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0521">NADP</keyword>
    <keyword evidence="11 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="12">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="DAPDH_C" evidence="13">
        <location>
            <begin position="120"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>NADP</name>
            <dbReference type="ChEBI" id="CHEBI:44409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>NADP</name>
            <dbReference type="ChEBI" id="CHEBI:44409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>NADP</name>
            <dbReference type="ChEBI" id="CHEBI:44409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NADP</name>
            <dbReference type="ChEBI" id="CHEBI:44409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NADP</name>
            <dbReference type="ChEBI" id="CHEBI:44409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="67"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 18">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>NADP</name>
            <dbReference type="ChEBI" id="CHEBI:44409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="71"/>
        </location>
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        <location>
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        <ligand>
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        <location>
            <begin position="90"/>
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            <name>NADP(+)</name>
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        </ligand>
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        <ligand>
            <name>NADP</name>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR025648"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR025648-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16654"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAD40410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="16">
        <source>
            <dbReference type="PDB" id="3WB9"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="17">
        <source>
            <dbReference type="PDB" id="3WBB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="18">
        <source>
            <dbReference type="PDB" id="3WBF"/>
        </source>
    </evidence>
    <sequence length="299" mass="32443" checksum="DD12557007F7B46A" modified="2004-10-11" version="1">MDKLRVAVVGYGNVGRYALEAVQAAPDMELVGVVRRKVLAATPPELTGVRVVTDISQLEGVQGALLCVPTRSVPEYAEAMLRRGIHTVDSYDIHGDLADLRRRLDPVAREHGAAAVISAGWDPGTDSIIRALLEFMAPKGITYTNFGPGMSMGHSVAVKAIPGVRDALSMTIPAGMGVHKRAVYVELEPGADFAEVERAIKTDPYFVRDETRVTQVESVSALMDVGHGVVMERKGVSGATHNQLFRFEMRINNPALTAQVMVAALRAAARQKPGCYTMIEIPVIDYLPGDREAWIRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI8</accession>
    <name>Q67PI8_SYMTH</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">STH1420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
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            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
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    </evidence>
    <sequence length="115" mass="12479" checksum="D29EC78721AE63AB" modified="2004-10-11" version="1">MDRRLWWRARLYRWGQAHDRWLRVGMLLGFVGLALTLALRTIAPQLWPAWQLLAMGGSLVLAGAGYALLGEQTTVVAYARGDSEQFPTVSRFASGLLASLGGAILAMTGLSAMMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU2</accession>
    <name>Q67PU2_SYMTH</name>
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            <fullName evidence="4">2-oxoacid:ferredoxin oxidoreductase beta subunit</fullName>
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        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">T / IAM 14863</strain>
        </source>
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            <name>[4Fe-4S] cluster</name>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PorB_KorB"/>
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    </dbReference>
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    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="47"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="PFO_beta_C" evidence="3">
        <location>
            <begin position="198"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12367"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAD40301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="31558" checksum="6CFA419F6E8BB2CB" modified="2004-10-11" version="1">MTMADFRTSEKSWWCPGCGDFGVLAALQKALVDLQIEPEKVAVVSGIGCSGKIGNYINSYNLHTVHGRALAAAQALKLANRELTVIAAGGDGDGFAIGMGHYMHALRRNVNITYIVMDNMIYGLTKGQTSPTSETGFTTKSTPTGNLESPVNQLQLALASGCGFVASGFSANQKQLVEIIKRAIQHPGFALVSTYSPCVTFNRVNTFDFFKQNLVNLDEDPSYNRHDRFAAMKKLMETQELVTGIIYEDPESVPYEDRVPGFREQGIVNQDWTMDPAVWEQIVAQYK</sequence>
</entry>
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    <accession>Q67PW2</accession>
    <name>Q67PW2_SYMTH</name>
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        <submittedName>
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        </submittedName>
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    </gene>
    <organism evidence="2 3">
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1296"/>
    <dbReference type="EnsemblBacteria" id="BAD40281">
        <property type="protein sequence ID" value="BAD40281"/>
        <property type="gene ID" value="STH1296"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1296"/>
    <dbReference type="eggNOG" id="COG4584">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2014103_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017895">
        <property type="entry name" value="HTH_IS408/IS1162_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50532">
        <property type="entry name" value="HTH_IS408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH IS408-type" evidence="1">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="14025" checksum="EE52FBA440E2A237" modified="2004-10-11" version="1">MVDHYRVLPAPPELWITSPHERTWASLGKSEVVDAQDQRDLRLKWEVGLSARQIARSLSISHSTVLDMLRRFECSGLSWPLPNIDDAELEAKLYPGNPQGQVERPLPDMAYIHRELARKGVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ9</accession>
    <name>Q67PZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydroorotate dehydrogenase electron transfer subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="5">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1259"/>
    <dbReference type="EnsemblBacteria" id="BAD40244">
        <property type="protein sequence ID" value="BAD40244"/>
        <property type="gene ID" value="STH1259"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1259"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_1_2_9"/>
    <dbReference type="OMA" id="RYMKCGI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="28081" checksum="07DB1738093C4076" modified="2004-10-11" version="1">MLAHEGDGEICRLTLHAPEIARRAAPGQFVQVHPVPVALRPSAPAIDPLLRRPFSLCEIRPEEGEITLIYRVVGRGTRVLSSVPAGAELDLLGPLGRSFPDPRAGRGLLVLVGGGLGIPPMAAAARWAAQARRDAVAILGARTARYLAGAREVAASGMPVVTVTDDGSAGQQGIVTEPLDRMLRHGAAAAAGRPGAANPVGEVWACGPEPMLAAVKELCTAAGVPCFVSVERFMGCGFGACLGCTVPRAGGGYLKTCQDGPVFPAEEVVLGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q15</accession>
    <name>Q67Q15_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1243</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195374.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1243"/>
    <dbReference type="EnsemblBacteria" id="BAD40228">
        <property type="protein sequence ID" value="BAD40228"/>
        <property type="gene ID" value="STH1243"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1243"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_4_1_9"/>
    <dbReference type="OMA" id="AHPSPGW"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="304" mass="34165" checksum="5E4EE8DF61C3CC24" modified="2004-10-11" version="1">MREYRITAEEGHPRIDVFLAGRPEIGLSRSRIQALIDEGQVTLNGAMPKARQAVNPGDVIVVRVPDPEPMELVPEDIPLDIVYEDEDILVVNKQRGLVVHPAAGHWTGTLVHALLDKVDDLEGIGGELRPGIVHRLDKDTTGLLVVAKSERAMASLQEQIRDHMVQRVYWALVHGNSMPDTGRIEAPIGRHPRDRKRMAVNPKTGRDAVTYFRVLERYRGYALLECRLETGRTHQIRVHLSFIGHPVVGDPLYGPRKPHLGMPPQALHARQLSFRHPASGEWMEFTVDPPADMMAVVQRLREEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q19</accession>
    <name>Q67Q19_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195370.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1239"/>
    <dbReference type="EnsemblBacteria" id="BAD40224">
        <property type="protein sequence ID" value="BAD40224"/>
        <property type="gene ID" value="STH1239"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1239"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_6_9"/>
    <dbReference type="OMA" id="DAVMNPY"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269322" evidence="1">
        <location>
            <begin position="31"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="83"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="576" mass="64263" checksum="9A4792791735A3FE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q22</accession>
    <name>Q67Q22_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sugar ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1236</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BMP lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1236"/>
    <dbReference type="EnsemblBacteria" id="BAD40221">
        <property type="protein sequence ID" value="BAD40221"/>
        <property type="gene ID" value="STH1236"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1236"/>
    <dbReference type="eggNOG" id="COG1744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038813_0_0_9"/>
    <dbReference type="OMA" id="LASMMKR"/>
    <dbReference type="OrthoDB" id="603910at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Bmp" evidence="4">
        <location>
            <begin position="49"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="355" mass="37811" checksum="A57B3C45A57AF807" modified="2004-10-11" version="1">MALTLSLLAGCGGSQQPQNQTPSTQPPAGQEQQPPQQQTQEPSKQVDFTVGMATDVGGLNDDSFNAAAYRGILQAEKDLGVKHIVIESQRQEDYITNLQTLIDGGADIVWGVGFMMQASIDEMAQANPDQWFGLIDEQVNQPNVASVLFKEQEGSFLVGYLAGKATKTNRVGFVGGVESPVIGRFEAGFRAGVMAANPDAVVDVVYAAAFDDPARGKDIALTMFNTGADVIFHAAGGTGQGVIEAAKEKNLFAIGVDSDQNYLAPNNVLSSMIKRVDVAVYDVIKMALEGNFPGGQVVRLGLAENGVGYSDTTLWDKMPEGTKAEVDKWAEAIKEGKVVVPETPQEADTWTPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q28</accession>
    <name>Q67Q28_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell-division initiation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">divIVA</name>
        <name evidence="8" type="ordered locus">STH1230</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195361.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1230"/>
    <dbReference type="EnsemblBacteria" id="BAD40215">
        <property type="protein sequence ID" value="BAD40215"/>
        <property type="gene ID" value="STH1230"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1230"/>
    <dbReference type="eggNOG" id="COG3599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076854_2_0_9"/>
    <dbReference type="OMA" id="QSIFVAQ"/>
    <dbReference type="OrthoDB" id="875822at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007793">
        <property type="entry name" value="DivIVA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794">
        <property type="entry name" value="PTHR35794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05103">
        <property type="entry name" value="DivIVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20918" checksum="206BE4008E5AE734" modified="2004-10-11" version="1">MGITPVDIANKEFPVRMRGYDRDAVDDFLDQVVQEFEALIRENASLREQVEHLNQRLEQYRSLEQTINRTLVLAEESAEEIRENARREAELLMNETRAQVERMLEAGQEKARQILADNADLIRVLDTLRTQVRALLKSQLEALDGLPDPLAHVAAARLSDAAPDRPARGIEPRNGLSAQAERNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q55</accession>
    <name>CARA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carA</name>
        <name type="ordered locus">STH1203</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q55"/>
    <dbReference type="SMR" id="Q67Q55"/>
    <dbReference type="STRING" id="292459.STH1203"/>
    <dbReference type="MEROPS" id="C26.A33"/>
    <dbReference type="EnsemblBacteria" id="BAD40188">
        <property type="protein sequence ID" value="BAD40188"/>
        <property type="gene ID" value="STH1203"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1203"/>
    <dbReference type="eggNOG" id="COG0505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_9"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112336" description="Carbamoyl-phosphate synthase small chain">
        <location>
            <begin position="1"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="171"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <sequence length="363" mass="38466" checksum="73FD5A197D26F625" modified="2004-10-11" version="1">MEDGTLFAGAGFGAPAVQAGEVVFNTGMTGYQEVVTDPSYYGQIVVMTYPLIGNYGVTAADGESRQPWIRGLVVKELCDRPSHWQAVGSLSDYLAGCGVPILAGLDTRALTRHLRSHGTMRGVIATLPEGAEPGPAQVAAWVETARAFRLEGAVRSVATAAPYRIPGPGPRVVAVDFGAKENILRELTARGCDVTVVPATASAEEVLSLRPEGVVLTNGPGAPTDVPEAVEMVRGLLSQGDLPIFGICLGHQIAALALGATTYKLPYGHRGANHPVKELATGRIHITSQNHGYAVAAESLPPEVEVTHVSLHDGTVEGLAHRRLPLFTVQYHPEACPGPRENRYLFDRFLALVDRSAVSTRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q95</accession>
    <name>Q67Q95_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RsgI N-terminal anti-sigma domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1163"/>
    <dbReference type="EnsemblBacteria" id="BAD40148">
        <property type="protein sequence ID" value="BAD40148"/>
        <property type="gene ID" value="STH1163"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1163"/>
    <dbReference type="eggNOG" id="COG4223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_459212_0_0_9"/>
    <dbReference type="OMA" id="HWEDSKE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024449">
        <property type="entry name" value="Anti-sigma_RsgI_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12791">
        <property type="entry name" value="RsgI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51849">
        <property type="entry name" value="RSGI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="RsgI N-terminal anti-sigma" evidence="9">
        <location>
            <begin position="9"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="272"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="322"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="594" mass="60106" checksum="C79A0BD31F07D5AC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA3</accession>
    <name>Q67QA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1155"/>
    <dbReference type="EnsemblBacteria" id="BAD40140">
        <property type="protein sequence ID" value="BAD40140"/>
        <property type="gene ID" value="STH1155"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1155"/>
    <dbReference type="eggNOG" id="COG1178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_1_0_9"/>
    <dbReference type="OMA" id="QAFWRVA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="66"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="350"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="552" mass="59694" checksum="F2ACE893D15F7B41" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD2</accession>
    <name>Q67QD2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative molybdopterin biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1126"/>
    <dbReference type="EnsemblBacteria" id="BAD40111">
        <property type="protein sequence ID" value="BAD40111"/>
        <property type="gene ID" value="STH1126"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1126"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_10_0_9"/>
    <dbReference type="OMA" id="MIYDALE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="7"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="27465" checksum="91F874695BA36E38" modified="2004-10-11" version="1">MHMTHACFTPAERERLDRASVLLIGCGGLGSAVAYALAAAGVGRIGLCDMDRVDLSNLQRQVLHHTADVGRPKVESAREKILGLRPGIQVDLHPVALTSENALDLFRQYDLIVDGSDNFATRYLVNDACVLTGRPQVHGSIFRFDGQVTTFVPGEGPCYRCVYPTPPPPGAVPSCAEAGVIGVLPGFIGSLMAMEALKLITGRGSPLIGRLLIFSALDMGVSEVQIRRNPECPVCGEHPTIHELMDYEQFCGGVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH0</accession>
    <name>Q67QH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1088</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195220.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1088"/>
    <dbReference type="EnsemblBacteria" id="BAD40073">
        <property type="protein sequence ID" value="BAD40073"/>
        <property type="gene ID" value="STH1088"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1088"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046705_0_0_9"/>
    <dbReference type="OMA" id="DKWIIFT"/>
    <dbReference type="OrthoDB" id="1335440at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024301">
        <property type="entry name" value="Amidase_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12671">
        <property type="entry name" value="Amidase_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269328" evidence="2">
        <location>
            <begin position="27"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase_6" evidence="3">
        <location>
            <begin position="306"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase_6" evidence="3">
        <location>
            <begin position="365"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="467" mass="51858" checksum="FE8C6FD717A073DA" modified="2004-10-11" version="1">MKRRYRPYLHAVVLTAILIGAQPRPAASQPVPAALAAVPPASQARLRAPGQRSPGQPSRPAEPRPSPSPDPGQPIRAAEPGQSPPAAEPGQSRRTREPGQPPRAPEPGQPSRAAEPGQSPAPAPSSESQPVDPPEPEKPDLTPRLREIYERRARRFLTDWEGPPLEEDFLLERKTAQWALLHEEGKYRYVKAWAEARGVRFVEARTRIWVKELKVTEDRARFYVAQTLQLGYQYPGEEAVNRFGVGSRHIVELHRTADGRWLIGLEWYSDPLGDETEAPAELPARRSSGNRGNSGLHAVTALAAQRYDRRGAVEYADTYCGLAAGCGNDHKYNPKFRNYMGEGGDCANFVSQALRYGGKLQMPLFTRADALIGHLRYAGKGDLAVRGDFGTVWRIAAGRPEGFRSFLKPGDVLGYEEKGKMTHVALITGFDSRGYPVANSHTADRYRVPFDLGWDRKTIYWFVAMRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK4</accession>
    <name>Q67QK4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative RNA nucleotidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1054"/>
    <dbReference type="EnsemblBacteria" id="BAD40039">
        <property type="protein sequence ID" value="BAD40039"/>
        <property type="gene ID" value="STH1054"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1054"/>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_3_1_9"/>
    <dbReference type="OMA" id="HRHKDVY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032810">
        <property type="entry name" value="CCA-adding_enz_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13735">
        <property type="entry name" value="tRNA_NucTran2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolyA_pol" evidence="5">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol" evidence="5">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="6">
        <location>
            <begin position="177"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_NucTran2_2" evidence="7">
        <location>
            <begin position="302"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="457" mass="50032" checksum="DB69B1FE75D882FD" modified="2004-10-11" version="1">MGGAARALRLGWGRWALPGRLRRALPPGGRTILARLTGAGHEAVVVGGAVRDLLLGLVPQDWDLATSARPAELQALFPEGRAVGAARETGTLLIPGDGTTYEITPYRAGRLEGDLARRDFTIDAMAATPEGRLIDPLGGRRDLAAGVVRACGRPEDRLAEDPLRMLRAVRLAAQFDLELDPALARAIAALAPRLQAVAPERIGAEFGRLLVTDRPAWGMQRLRELGLLHQFAPELLEMVGVEQNRYHAFPVWEHSLMALALVPPALHLRLAALLHDVAKPRCLSVDADGERHFYGHEAVGAELADRLLRRLRFDNATREKAVHLVRRHMDLHLDGPVTDAALRRMVRRIGVEHLDDLVQLRRADRLASGKREGDLGPDTMAILDGIRRVLAEDAALKVTDLAVDGHDVMAAFGRGPGPYVGQVLEALLEEVLDRPELNRREALLRRLQEMADGGWKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL4</accession>
    <name>Q67QL4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1044"/>
    <dbReference type="EnsemblBacteria" id="BAD40029">
        <property type="protein sequence ID" value="BAD40029"/>
        <property type="gene ID" value="STH1044"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1044"/>
    <dbReference type="HOGENOM" id="CLU_2169790_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="110" mass="11945" checksum="AE27E9880D5F9A0F" modified="2004-10-11" version="1">MAVRVAPDGATQPGRAARRASEREVQARVHRQMGITAAVMGVAAAGVGFWWIGRVFGVGPLEALQAAWPLFLLVLVILALGMLLSLGAARAAWLTERFLARRRQRKVDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU0</accession>
    <name>Q67QU0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-(Acyl-carrier protein) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH968"/>
    <dbReference type="EnsemblBacteria" id="BAD39953">
        <property type="protein sequence ID" value="BAD39953"/>
        <property type="gene ID" value="STH968"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH968"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_9"/>
    <dbReference type="OMA" id="AMHRTNI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="24557" checksum="71467F003B5F54DC" modified="2004-10-11" version="1">MTGASRGIGRAVALELAAAGAAVVVGYHRQREQAEAVAAAIRAAGGAAEPLQVDVRDPAACEAAVSGTVDRFGRIDILVNNAGTALEKLLVDTTPAEWNDLVAVHLTGMYACTRAALPHMLSRRYGRIITISSIWGITGAAGEVAYSAVKAGQNGFTRALAQEVGPFGVTVNAVAPGAIDTEMNSFLQGEELREWLSRVPVGRLGTPEEVAALVRFLAGPQAGFITGQVISPNGGVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV3</accession>
    <name>Q67QV3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphocarrier protein HPr</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH955</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195087.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH955"/>
    <dbReference type="EnsemblBacteria" id="BAD39940">
        <property type="protein sequence ID" value="BAD39940"/>
        <property type="gene ID" value="STH955"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH955"/>
    <dbReference type="eggNOG" id="COG1925">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136230_2_2_9"/>
    <dbReference type="OMA" id="MSLAIGH"/>
    <dbReference type="OrthoDB" id="404807at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001020">
        <property type="entry name" value="PTS_HPr_His_P_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00369">
        <property type="entry name" value="PTS_HPR_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPr" evidence="3">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="85" mass="9464" checksum="266E7E892755D85A" modified="2004-10-11" version="1">MQQFTHTIRDELGLHARPAALLVRLSTQYKSRITLTKGEKVADAKGIFAVMTLGVKCGDTLTVTVEGEDELEAAEALREFFQRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R05</accession>
    <name>Q67R05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH903"/>
    <dbReference type="EnsemblBacteria" id="BAD39888">
        <property type="protein sequence ID" value="BAD39888"/>
        <property type="gene ID" value="STH903"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH903"/>
    <dbReference type="eggNOG" id="COG4769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787380_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010898">
        <property type="entry name" value="Hpre_diP_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038690">
        <property type="entry name" value="NusG_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024045">
        <property type="entry name" value="NusG_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07456">
        <property type="entry name" value="Hpre_diP_synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07009">
        <property type="entry name" value="NusG_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="NusG_II" evidence="3">
        <location>
            <begin position="245"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="352" mass="35417" checksum="8848734E500E23BD" modified="2004-10-11" version="1">MPARRPRRSQSQHVGVTPLSGRRTSEPKVTRSFSTRQLTRLSLLTAAGLALALLERQLPPPAPVPGVRWGLANAATLAALALDGPAAGLLVWFCRFILAQIFAGTLFGPAFFVGGAGGLLAWAAMALFARGRRLGPAGVSAAGAVAHHVGQLAAAAAVTSTPGVLALLPLLLVLGPPVGLLTGALVGLLLRRLEAAGALGGAGAGDVARATAPDGSAPRAAVSGGRSGLIRASDLALAGGVAVLAGALVLGRGWWAGSRGPAAQAVVTVAGAPWRTLDLRQDGLYPVEPAGGHLVVEVADGAVRVREADCPDQVCVLTGWISSPGDMIVCVPYRVVIQITGDSGVGPDAILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R50</accession>
    <name>Q67R50_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH858"/>
    <dbReference type="EnsemblBacteria" id="BAD39843">
        <property type="protein sequence ID" value="BAD39843"/>
        <property type="gene ID" value="STH858"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH858"/>
    <dbReference type="HOGENOM" id="CLU_1365622_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="21564" checksum="56801F68FC9E719A" modified="2004-10-11" version="1">MMEPVDAAGDAGRAALPPVQTDPVTSAEEALARIPPAALEGRTFDALLVEDYGHRSPDRQWHERECSTWIVQILHPSSGVTFLIDAATGELNAFVQSEAQAPAKLPKSAQDAVDLVFEQWGHLPYIRQFPSAIGSSESPLRLPDESEIDRFSALSTTAATADRRGEYLVTPPRTGRKTAAKDRPGGSSPCIPPVSSRRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R75</accession>
    <name>Q67R75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aspartate ammonia-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194966.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH833"/>
    <dbReference type="EnsemblBacteria" id="BAD39818">
        <property type="protein sequence ID" value="BAD39818"/>
        <property type="gene ID" value="STH833"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH833"/>
    <dbReference type="eggNOG" id="COG1027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021594_4_1_9"/>
    <dbReference type="OMA" id="EICENYV"/>
    <dbReference type="OrthoDB" id="734949at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="2">
        <location>
            <begin position="12"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="3">
        <location>
            <begin position="407"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="469" mass="50622" checksum="DB71FDB6F00842D2" modified="2004-10-11" version="1">MQQTRTERDLLGEKQVPADAYYGIQTTRALENFRITGQRLHPALVRGLAQVKKAAAQANHQLGYLPDDVARAIVQACDEILAGRLADQFPIDPVQGGAGTSANMNANEVIANRAIEILGGRKGDYSLVSPNDHVNFAQSTNDAFPTALRLALMERADVLVESLVGLEEALRAKAAEFDDVVKVGRTHLQDAVPIRLGQEFSAWCAAVERGRRRIQQAVEALCEVNMGATAVGTGLNADPAYPPLVIELLSRNTGRSLRPPADLVDATQNVDPLVALSGSLKALAVSLSKIANDIRLMASGPRCGLYEIRLPEMQPGSSIMPGKVNPVMAEVLNQTCFLVIGLDLTVTLAGEAGQLELNVMEPVMGHALFTSIDALESVVRLFTDRCVRGIQANRERCEELVNRSIGLVTAINPIVGYKNASEVAREATLTGRPVREIVLEKGLLTAEEIDEILSPKHLTTMGIARRTHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R76</accession>
    <name>Q67R76_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-methionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH832</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-methionine = 2-oxobutanoate + methanethiol + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23800"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="EC" id="4.4.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family. L-methionine gamma-lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.11" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194965.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH832"/>
    <dbReference type="EnsemblBacteria" id="BAD39817">
        <property type="protein sequence ID" value="BAD39817"/>
        <property type="gene ID" value="STH832"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH832"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_9"/>
    <dbReference type="OMA" id="SMTHSVV"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018826">
        <property type="term" value="F:methionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006237">
        <property type="entry name" value="L-Met_gamma_lys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01328">
        <property type="entry name" value="met_gam_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="401" mass="43004" checksum="91BC18B59EC6EAD2" modified="2004-10-11" version="1">MEQTRQPGLATRCVHAGQRPDPLTGAISPPIYQTSTFAFASLEQGAARFAGEEPGYIYTRLGNPTVAALEAKIADLEGGEAAVAFGSGMAAIAAVVTALVRAGDHVLFGNPIYGCTYDFLMDVMARFGVEATPVDPARPGQVERAIRPNTRLLLFETPANPTLRLADIRALSDLAHRHQITVVVDNTFMSPYLQRPLEHGADMVVHSATKFIGGHGDVVAGLAVGPAEVMARVRSTTLKNFGGILSPFDAWLLLRGLKTLAIRMERHSENALKVARYLEQHPLVMRVYYPGLESSPYHELARRQMDGFGGLLSFELMGGMEAGRTLLNHVRLCTVAVSLGDTDTLIQHPASMTHAVVPPEARAAAGISDGLVRLSVGLEDADDIIADLDQALAAVGRAQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA8</accession>
    <name>Q67RA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched-chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194933.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH800"/>
    <dbReference type="EnsemblBacteria" id="BAD39785">
        <property type="protein sequence ID" value="BAD39785"/>
        <property type="gene ID" value="STH800"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH800"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="QHIRKTP"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="264" mass="29113" checksum="9F7F25069856C81A" modified="2004-10-11" version="1">MALLELRQVTMRFGGIVAVDRLDLAVEEGTIHGLIGPNGAGKSTVFNLISRIYDPVGGSIAFAGKDLLRMAPHDVIHHGIARTFQNVELFRGLTVLGNLLVGQHSRIRYGLLASALGNLVPAVRREEREAEERALEVLEFLGIAAYKDAYPQLLPYGIQKRVEFGRALVAQPRLILLDEPAAGMNPQETLELAELIREVRDRYRATVLLVEHDMSLVMRICERITVMDFGRKIAEGTPAEVQSDPAVIRAYLGEEDDEEGAAHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD6</accession>
    <name>RBSD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-ribose pyranase</fullName>
            <ecNumber evidence="1">5.4.99.62</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbsD</name>
        <name type="ordered locus">STH772</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of beta-pyran and beta-furan forms of D-ribose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-ribopyranose = beta-D-ribofuranose</text>
            <dbReference type="Rhea" id="RHEA:25432"/>
            <dbReference type="ChEBI" id="CHEBI:27476"/>
            <dbReference type="ChEBI" id="CHEBI:47002"/>
            <dbReference type="EC" id="5.4.99.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbsD / FucU family. RbsD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.62" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194905.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RD6"/>
    <dbReference type="SMR" id="Q67RD6"/>
    <dbReference type="STRING" id="292459.STH772"/>
    <dbReference type="EnsemblBacteria" id="BAD39757">
        <property type="protein sequence ID" value="BAD39757"/>
        <property type="gene ID" value="STH772"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH772"/>
    <dbReference type="eggNOG" id="COG1869">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135498_0_0_9"/>
    <dbReference type="OMA" id="IIRTGEC"/>
    <dbReference type="OrthoDB" id="1750843at2"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00888"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062193">
        <property type="term" value="F:D-ribose pyranase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01661">
        <property type="entry name" value="D_rib_pyranase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023064">
        <property type="entry name" value="D-ribose_pyranase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023750">
        <property type="entry name" value="RbsD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007721">
        <property type="entry name" value="RbsD_FucU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37831">
        <property type="entry name" value="PTHR37831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05025">
        <property type="entry name" value="RbsD_FucU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102546">
        <property type="entry name" value="SSF102546"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000346286" description="D-ribose pyranase">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01661"/>
        </source>
    </evidence>
    <sequence length="131" mass="13898" checksum="3B0DFA2F4BF7AC24" modified="2004-10-11" version="1">MKKTTLLNQALSEVVAGMGHGDLLVIGDYGLPCPKGVRRIDLALRPGIPAFLDVVETILAELQVEAAVVARETAERNPAVQEGLTRLLGGVPVTTVSHEELKEISARAVALVRTGECTPYANVILRAGVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI0</accession>
    <name>Q67RI0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH728"/>
    <dbReference type="ESTHER" id="symth-q67ri0">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39713">
        <property type="protein sequence ID" value="BAD39713"/>
        <property type="gene ID" value="STH728"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH728"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924160_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="68"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="301" mass="32273" checksum="255526D78E8B2AB3" modified="2004-10-11" version="1">MRQRSDLTVHTDPIAITSETHSLAGTLVLPDGAGNDRPVPAALIVGGPGPLPLERRAQDGTPNWPLRWAEAFGAAGLACLCYDQRGSGESTGQYEDADWDDLYGDAAAAAEILRLQPEVSRVIAVAWADAVGFALRLAAEGKVDGLILLAAGAHTAEERYREQVRRLAAGRGLSDRVVELRVRQWQAQLQAVRDRVAAGERVAETDIGGQRIVTNLRRFLAVHEFDPMAAARQVATPALLLHGAADTVVPPAESELLAETLAGPVERRVYPGEGHFLYRSDQAVADAVGWMQRRAAGITAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI2</accession>
    <name>Q67RI2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative molecular chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH726</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194859.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH726"/>
    <dbReference type="EnsemblBacteria" id="BAD39711">
        <property type="protein sequence ID" value="BAD39711"/>
        <property type="gene ID" value="STH726"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH726"/>
    <dbReference type="eggNOG" id="COG2076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133067_1_2_9"/>
    <dbReference type="OMA" id="FEIAWAI"/>
    <dbReference type="OrthoDB" id="1990300at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045324">
        <property type="entry name" value="Small_multidrug_res"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="PTHR30561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00893">
        <property type="entry name" value="Multi_Drug_Res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="11464" checksum="82AFD87CA37A6ED1" modified="2004-10-11" version="1">MAWLYLFVAGAMEIAWSTAMKYSEGFTRFWPSVATIVLSLTSFVLLSQAMKTLPLGTAYGVWTGIGALGSALVGIVFLGEPREPLRLLCIFLIFAGIVGLKLTSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS7</accession>
    <name>Q67RS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH631"/>
    <dbReference type="EnsemblBacteria" id="BAD39616">
        <property type="protein sequence ID" value="BAD39616"/>
        <property type="gene ID" value="STH631"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH631"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="YATHNIS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007891">
        <property type="entry name" value="CHASE3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05227">
        <property type="entry name" value="CHASE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="203"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="303"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="619" mass="64297" checksum="E10955668629881D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV9</accession>
    <name>Q67RV9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH599</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194732.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH599"/>
    <dbReference type="EnsemblBacteria" id="BAD39584">
        <property type="protein sequence ID" value="BAD39584"/>
        <property type="gene ID" value="STH599"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH599"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="6"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="34320" checksum="C0D2057987DEB2BD" modified="2004-10-11" version="1">MSEPVLKLDEIVVLRRTDPTGPFGLWGARPVRAVDGVSLTVDREETLGLMGGSGAGKTTLAQAATLRLPLHRGNLLMLGEDVRRLDRKRARRRLQLVPQDPRESLDMDRRVDEQLDQLVRAYELPDGGARIRRALERVGLPPEQFLKRTPRQMCGGEQQRLAIAQALALNPLLVALDEPVSGVDPRLREELLDLLADVQREQGVAYLLISQDLRVIGRLARRVAIMHLGRICEVGPTEQVLMNPRHPYSRRFLGKEEGALPPDENTAGRVFAGCPWAPHCPHAVERCTRERPAEREVAPGHLAACHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RY0</accession>
    <name>Q67RY0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldedh domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH578</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194712.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH578"/>
    <dbReference type="EnsemblBacteria" id="BAD39563">
        <property type="protein sequence ID" value="BAD39563"/>
        <property type="gene ID" value="STH578"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH578"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046407_0_0_9"/>
    <dbReference type="OMA" id="CVWVAPW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="21"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="477" mass="50549" checksum="8715C058FE1EE9BA" modified="2004-10-11" version="1">MREIPIWRGGRERTSLDVRLLRDYRGEPLAKVAVAPALLVHRAVAELRAAAEPEADLTALWDRIAAAGRLLDAAELGGCSPEEHARLVTLATGAPLTDSRRALAELARGMAQVREALTWQAPGGTPEPFLTGRIEPAPGKSCGWAPVGRVLGFVAPSNHPAVHLTWVLALAMGWVVAVRPGADDPFTPWRVLLALREAGFPAERIALLPGGHDLVPALVEHCDRTVAYGGPALESLLGRDPRVLFNGPGRSKVLVDTDAVGAAPGDPTPEPNGQPSGLRTHKPASSSTSAEDPVTFLVDCILHDGGRKCTCASAVLLRGDAPGLLEAVAERLARLPLLDPLDPAARVPAWKDPAAARLTPGEAVEVDGLTFVRPALVLGVDPADPRFGAEIPAPWATGLRLPAGADPLPLLRGSLAVTLMTKEAALVRRCLREPSIQKLFVGPVPPWHTEPGAPHKGRLADFLFTAKAYREAVIPWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ9</accession>
    <name>Q67RZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH559"/>
    <dbReference type="EnsemblBacteria" id="BAD39544">
        <property type="protein sequence ID" value="BAD39544"/>
        <property type="gene ID" value="STH559"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH559"/>
    <dbReference type="eggNOG" id="COG0520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_766715_0_0_9"/>
    <dbReference type="OMA" id="VGAESKF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="362" mass="38456" checksum="BE7594DDC10CB152" modified="2004-10-11" version="1">MTHPLPALSPAEAATLQWRLVDLIHTHFDGHEILQAGDLGVVPGLGRPRTTARVERVLADFFGGEAAALVRGAGTGAIRAALQAIPGVRRILVHRAPIYSTTRSTIEAMGLDVLQADFHDPEELSRKAREADLALVQHARQRLDDRYALGEVIRTLRKAAPGLPIITDDNYAVCKVPAIGVQLGATASTFSLFKLLGPEGVGCVVGAADVVERIHRAAYSGGLQVQGPEAMDALRSLVYAPVALALQAQVVQEVVDRLNAGEVPGVACAAVANAQSRVALVQLCEPIADRVLEVAPRFGAAPHPVGAESRYEVAAMFYRASGTMLADRPDLAPFLLRINPMRAGADTVIRVLRMSVEAAVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S20</accession>
    <name>Q67S20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194672.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH538"/>
    <dbReference type="ESTHER" id="symth-q67s20">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39523">
        <property type="protein sequence ID" value="BAD39523"/>
        <property type="gene ID" value="STH538"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH538"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_8_9"/>
    <dbReference type="OMA" id="HGMCTVE"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="25"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="25668" checksum="2E6D324688E5B68C" modified="2004-10-11" version="1">MPSLPTPAEGTLWYAESGPADGRPLLLLHAALQTHESMAPLAEELAPLGLRLIAVDLPGHGRSAHAGPLTIRTMADAVAGLIERLGLDRPLAAGYSLGGIVGIELALRGLVSGLVVLASRIQPAGDARATFAPEAIRRRSPLWARQLAEKHTVTPWEALAAALGEELASWPGFDPQALAALSVPVLVVQGDRDQMVPLEQGQELARLAPQGRFCLVPRAGHPELLYRADARAAVRAFLANFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S29</accession>
    <name>Q67S29_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphate starvation-induced protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH529</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194663.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH529"/>
    <dbReference type="EnsemblBacteria" id="BAD39514">
        <property type="protein sequence ID" value="BAD39514"/>
        <property type="gene ID" value="STH529"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH529"/>
    <dbReference type="eggNOG" id="COG1702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051654_0_0_9"/>
    <dbReference type="OMA" id="PAQMKMV"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="4">
        <location>
            <begin position="111"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="340" mass="37786" checksum="37B82272610FAD93" modified="2004-10-11" version="1">MDETQERRILIADNDRARELFGLNDSHLRFIEEGFDVTIVPRGNEVIIKGQEDEVGRVVRLFNRLTAIQERGGRVTERDVRYAVRLAREGDEKPLADALTDVIVTTYRGKQIRPKTIGQKEYVDAIRRSGVTFGIGPAGTGKTYLAMAVAVAAFKAKEVARIILTRPAVEAGEKLGFLPGDMQQKVDPYLRPLYDALFDIMGSETFERLMDRGVIEVAPLAFMRGRTLDDSFIILDEAQNTTPEQMKMFLTRMGFGSKVVVTGDITQIDLPEGTRSGLVDVQRVLRNVPGISFYYLTERDVVRNDLVQLIIKAYEAAGGGGTGSHPPRPRAGRPNGSETQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S44</accession>
    <name>RS21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsU</name>
        <name type="ordered locus">STH514</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194648.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S44"/>
    <dbReference type="SMR" id="Q67S44"/>
    <dbReference type="STRING" id="292459.STH514"/>
    <dbReference type="EnsemblBacteria" id="BAD39499">
        <property type="protein sequence ID" value="BAD39499"/>
        <property type="gene ID" value="STH514"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH514"/>
    <dbReference type="eggNOG" id="COG0828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159258_3_2_9"/>
    <dbReference type="OMA" id="FKRSCQK"/>
    <dbReference type="OrthoDB" id="2088764at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00358">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_S21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018278">
        <property type="entry name" value="Ribosomal_S21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038380">
        <property type="entry name" value="S21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109">
        <property type="entry name" value="PTHR21109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00976">
        <property type="entry name" value="RIBOSOMALS21"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01181">
        <property type="entry name" value="RIBOSOMAL_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000178390" description="30S ribosomal protein S21">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="58" mass="6998" checksum="38725B154F8F2628" modified="2004-10-11" version="1">MSEVKIGKNESLDSALRRFKRQLQRAGVLAEIRKREHYEKPSVKRKKKSEAARRRKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S50</accession>
    <name>Q67S50_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="6">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH508</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194642.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH508"/>
    <dbReference type="EnsemblBacteria" id="BAD39493">
        <property type="protein sequence ID" value="BAD39493"/>
        <property type="gene ID" value="STH508"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH508"/>
    <dbReference type="eggNOG" id="COG1385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067442_3_0_9"/>
    <dbReference type="OMA" id="TQWKGER"/>
    <dbReference type="OrthoDB" id="1490892at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="26218" checksum="22627462865F98FD" modified="2004-10-11" version="1">MTPRFFLERVEGDEVVLPRADSHHLLRVLRAGPGTPFVVLSGGMAYDCVLHRVDDGLARGRIRAAEPAAGEPAIRITLFQGLAKGEKMELVLQHGTELGIAEFVAVAAARSVVRLDERKAAQRTERWQRIAREAAEQSRRGAVPTVAPPHTWKEAVARIPEFDLALVAWEGGGEPLRAALEARPEARDIAVFIGPEGGLTAEEVELARQAGAVPVTLGPRILRTETAPLAAVAAILYARGEWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S81</accession>
    <name>Q67S81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194611.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH477"/>
    <dbReference type="EnsemblBacteria" id="BAD39462">
        <property type="protein sequence ID" value="BAD39462"/>
        <property type="gene ID" value="STH477"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH477"/>
    <dbReference type="eggNOG" id="COG1950">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159834_0_0_9"/>
    <dbReference type="OMA" id="VSWIVPG"/>
    <dbReference type="OrthoDB" id="1925503at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007165">
        <property type="entry name" value="Phage_holin_4_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37309">
        <property type="entry name" value="PTHR37309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04020">
        <property type="entry name" value="Phage_holin_4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269353" evidence="2">
        <location>
            <begin position="27"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="109" mass="11378" checksum="0480A2DEA2A298C1" modified="2004-10-11" version="1">MMGAIVRFVVSAVVLLVVAWLTPGFAVNGFWAALFASLVIAGLGWVAQAMLGRGASPAGRGVAGFIAAAVIIYLTGWLFPGWLTVTWWGALIAAFLIGLADAFVPTELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S92</accession>
    <name>Q67S92_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4129 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194600.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH466"/>
    <dbReference type="EnsemblBacteria" id="BAD39451">
        <property type="protein sequence ID" value="BAD39451"/>
        <property type="gene ID" value="STH466"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH466"/>
    <dbReference type="HOGENOM" id="CLU_831343_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="DUF4129"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269521" description="DUF4129 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4129" evidence="2">
        <location>
            <begin position="245"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="334" mass="35627" checksum="23DAA2EB5EEE00C1" modified="2004-10-11" version="1">MRRALATLLLVLWLPSAAASAPLPAGAYAGRLEEAAALLDEAERLAQQGDETAARRRIYAAADRLSGVDAVWADAGDVEADLTDLMDSLRGAAEDPEALSRARGLLAEHLHAAADLVEAEPVHAPGARASLDRALAQVAGQSLLDRAREWVLGLFTRPLQGKELGPVPPSVWWIGGAVGALALAWAGFALYRGLTGHGAGTEGTWREGRRTEPARPPTPAELLHAAQEAADRSDYLAAVRLSHLALLQHLDGLGIIRYQPAQTDREHERQLARRRPDLVPALRSLHDLLEGLLYGGRPARADEFRQAESLVMQLWREGDATSGSAATPGPSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S96</accession>
    <name>Q67S96_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH462"/>
    <dbReference type="EnsemblBacteria" id="BAD39447">
        <property type="protein sequence ID" value="BAD39447"/>
        <property type="gene ID" value="STH462"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH462"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048408_0_0_9"/>
    <dbReference type="OMA" id="RVMKMPF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF58" evidence="1">
        <location>
            <begin position="185"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="48069" checksum="D44B46D2E11EEAD0" modified="2004-10-11" version="1">MRFSPTPRLPLLLALAALPALFPGLLWLSLALAAALTGVALADLAASRRTIEAERRVENPLSLGEENPVRLAFRCQGAPVTLEVCDDLPLHLEPAGEWPVVRLEEGAWVEVGYRVRPGRRGRYAIGPLHGRYRSRLGLWNRMITWPITDEVRVYPNLLAVRQYEVAMRQGRQMEGLKRARLRGAGTEFESLREYQEGDEFRAINWPATARRGSLVTNLYQVDRSQPIMLLVDAGRLMIPQIQGLSRLDYVLNAAMLLATVAAERGDQVGLMLFGGEVKAFVPPRKGRRQVMAMLESLYDLQPEQVEPDYGRMLGWFAARHRRRSLLVLFTDLVDPEINRGLTAHLSALAARHLVLVVTLTDPALLALSRCIPENAQQAYEKATALEVLAQRAETRARLQRSGVLVIDVPPSSFSAAVVNQYLQIKEQGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA4</accession>
    <name>Q67SA4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194588.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH454"/>
    <dbReference type="EnsemblBacteria" id="BAD39439">
        <property type="protein sequence ID" value="BAD39439"/>
        <property type="gene ID" value="STH454"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH454"/>
    <dbReference type="eggNOG" id="COG2823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1209287_0_0_9"/>
    <dbReference type="OMA" id="SAMDINC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014004">
        <property type="entry name" value="Transpt-assoc_nodulatn_dom_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00749">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BON" evidence="1">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="BON" evidence="1">
        <location>
            <begin position="81"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="24961" checksum="FDABDB7A41A4E98E" modified="2004-10-11" version="1">MPEQTFEHDEQLAKQVKEALLAQTDSAGIDVQVSARDGTVRLHGVVDALSHKSAAEEIARRVPGVRRIDNDITVANDETLSDRDLEGAVSSRLADVPEYSRIGAQVEKGVVTLMGEARTYEDLEAAVRLVEGVPGVREVRSDQVKVGVGEEEDDADVSRAALRLLGRMGYDPDRFQLYCADGVLHVKGIVPGREDRSRIKAALRGIRGVAKLEALLVAEDEMLADEEVH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC9</accession>
    <name>RL21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplU</name>
        <name type="ordered locus">STH429</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194563.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SC9"/>
    <dbReference type="SMR" id="Q67SC9"/>
    <dbReference type="STRING" id="292459.STH429"/>
    <dbReference type="EnsemblBacteria" id="BAD39414">
        <property type="protein sequence ID" value="BAD39414"/>
        <property type="gene ID" value="STH429"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH429"/>
    <dbReference type="eggNOG" id="COG0261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061463_3_2_9"/>
    <dbReference type="OMA" id="HRQEITR"/>
    <dbReference type="OrthoDB" id="1949059at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018258">
        <property type="entry name" value="Ribosomal_L21_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01169">
        <property type="entry name" value="RIBOSOMAL_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000269404" description="50S ribosomal protein L21">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <sequence length="104" mass="11442" checksum="4C81AD095B06150C" modified="2004-10-11" version="1">MTYAIVETGGKQYRVQEGDTLRVEKLTAGEGETVVFDKVLAISRDGKVTVGTPYIEGAKVTAKVAAHGKGPKIIVFKYRNKTNYRRKTGHRQPFTAITIESIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM5</accession>
    <name>Q67SM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Restriction endonuclease R.SthI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194467.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH333"/>
    <dbReference type="REBASE" id="10265">
        <property type="entry name" value="StpORF334P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39318">
        <property type="protein sequence ID" value="BAD39318"/>
        <property type="gene ID" value="STH333"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH333"/>
    <dbReference type="eggNOG" id="ENOG5033VEG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_834052_0_0_9"/>
    <dbReference type="OMA" id="TICSAMI"/>
    <dbReference type="OrthoDB" id="748390at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019071">
        <property type="entry name" value="Restrct_endonuc_II_XcyI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09571">
        <property type="entry name" value="RE_XcyI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="37024" checksum="BA2F61567613BB16" modified="2004-10-11" version="1">MASARKPPKPLNLPSPLVLPDPAGAIEFGASLDALRQLFLVQALLATVKTLDLNVLNAELAQYVPAHRLQEMAGVGLRAEIVYPVPCLIEANPRLIAYYRLLLGYSAKRLYRMNSPLGPFETLEKKGVINPRLRPHIPTICSAMIASAEKLVDGLGIQRITRELLDDLQLLTLGSQLQGARNVAIGMAGMHAVRLIIEDIVAPYITKSDANSIVLTNSAGRTVTIRIGADPDVRIEEKVGRNVKRTLSIEVKAGEDNSNIYNRLGEAEKSHNKAKKKGFTEFWTIVNTSNIDLVKARVQTPTTNEFFLLRLLKDKSTPEYADFRQRIEALCGIPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ3</accession>
    <name>Q67SQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194440.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH305"/>
    <dbReference type="EnsemblBacteria" id="BAD39290">
        <property type="protein sequence ID" value="BAD39290"/>
        <property type="gene ID" value="STH305"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH305"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_4_1_9"/>
    <dbReference type="OMA" id="THARGHA"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002525">
        <property type="entry name" value="Transposase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01548">
        <property type="entry name" value="DEDD_Tnp_IS110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEDD_Tnp_IS110" evidence="2">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="431" mass="48650" checksum="901BCB00B54101B7" modified="2004-10-11" version="1">MQLKQRARKLALVRGEPTVVVGIDIAKRKHWAWVVDGRTEVPVGSPFHFQNSREGFDRLLGKIARAKEKAGATRVVVGMEPTGHYWKPLAWYLQQVGFTVVIVNPAHVKASKELDDNSPTKNDRKDAWLNAQLVLQGRFSVPYLPDGVYAELRILTQARQQHRRKLNAALNQLQALLDEYFPEYTEVFADPLGQASLYVLQYRPFPQDVLAVPVTQLAAELRAASNRRVGLRRAQDLQEAARRSIGVPYGLDAARLQLRQILQEVIFWKERLAETEAAMAEALEKTGLAQYLLSVPGVGVVTAAAFLGEVGDLRRYADWRQLRKLAGYNLTENTSGQRDRSRTPISKRGRPGLRHVLYQMAMTAAAKNREFRALYKHFRTRQQNPLKGKQALVALACKLLRVLFTLVRKQCSYDPARALGAWRAEQLGLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU3</accession>
    <name>Q67SU3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH265"/>
    <dbReference type="EnsemblBacteria" id="BAD39250">
        <property type="protein sequence ID" value="BAD39250"/>
        <property type="gene ID" value="STH265"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH265"/>
    <dbReference type="eggNOG" id="COG3267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024125_1_0_9"/>
    <dbReference type="OMA" id="HEMSDAM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="228" mass="26064" checksum="A2DF72C40F9659C5" modified="2004-10-11" version="1">MGSGKTTAIRTLYDLLDRSRNPFVYIADSKLTPTAFYRDVLTQLGVAAPYHFLSREARRLFEQTILDGYRLHGLQPVIVLDEAHLLPHAMLQEVRFLLNFHLDSACPMTFLLVGQSELRGMLRLRTFEAIAQRVQVRYHLGPLSDEESVAYIRHHLRRTGVDHDIFSEQALQKIAAESRGLPRVINTLCTNCLLDACSREQRFVDEANVDRVLFELQDLQTAKGSSPW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC0</accession>
    <name>ATPD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit delta</fullName>
            <shortName evidence="1">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpH</name>
        <name type="ordered locus">STH88</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase delta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194223.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TC0"/>
    <dbReference type="SMR" id="Q67TC0"/>
    <dbReference type="STRING" id="292459.STH88"/>
    <dbReference type="EnsemblBacteria" id="BAD39073">
        <property type="protein sequence ID" value="BAD39073"/>
        <property type="gene ID" value="STH88"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH88"/>
    <dbReference type="eggNOG" id="COG0712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085114_1_1_9"/>
    <dbReference type="OMA" id="MVDNIQD"/>
    <dbReference type="OrthoDB" id="1937493at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000371173" description="ATP synthase subunit delta">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <sequence length="186" mass="21084" checksum="CF67F5B358CA64F3" modified="2004-10-11" version="1">MMNHAVARRYARALFELAQEKGLLDQVNRELELVVSMYETDSYLRAFMNDQRISPSQKRKVLASVLEGKVSPLVLHFLYVVVQKRREPHLPSMYRAFQDLANEAMGVVEVEVRSAVPLAEETARNLEAKLTTKLGKRVKLRTQVAPELIGGLAVRVGDTLMDGSVRTRLRRMHERLISAQSNVIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD2</accession>
    <name>Q67TD2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Low molecular weight protein-tyrosine-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH76</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194211.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH76"/>
    <dbReference type="EnsemblBacteria" id="BAD39061">
        <property type="protein sequence ID" value="BAD39061"/>
        <property type="gene ID" value="STH76"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH76"/>
    <dbReference type="eggNOG" id="COG0394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_1_2_9"/>
    <dbReference type="OMA" id="AFFPQKA"/>
    <dbReference type="OrthoDB" id="2062716at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="4">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="156" mass="16659" checksum="48CA08897993D14F" modified="2004-10-11" version="1">MRVERVLLVCSGNTCRSPMAAALLRALWRRADPGWDLQVDSAGIGAFPGLPAAPNAVAAMKARGIDLSGHRSQAVTDLEGYDLVLGMTRAHRDALRTRFPALAGRIFTLAEYAGVGGDLSDPFGGPLQAYEETAKALEEQLQAVVERIRREGSAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG1</accession>
    <name>Q67TG1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH47</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH47"/>
    <dbReference type="EnsemblBacteria" id="BAD39032">
        <property type="protein sequence ID" value="BAD39032"/>
        <property type="gene ID" value="STH47"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH47"/>
    <dbReference type="HOGENOM" id="CLU_2866162_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="6929" checksum="C1321DD73B93EFC9" modified="2004-10-11" version="1">MQVQHTSSWLELPDLCVLQVATVSDGTLVVVVEGVATGARCPKCCSWTNRVHQLLSPGLFVNMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q3V819</accession>
    <name>Q3V819_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyridoxal phosphate biosynthetic protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PdxA family. PdxA2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2236"/>
    <dbReference type="EnsemblBacteria" id="BAD41221">
        <property type="protein sequence ID" value="BAD41221"/>
        <property type="gene ID" value="STH2236"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2236"/>
    <dbReference type="eggNOG" id="COG1995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2095636_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="PTHR30004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="12630" checksum="D536BDE0E5F832DB" modified="2005-10-11" version="1">MHVSLRQAIDLVTADRGRRVIGVAHRTLRRMGFQHPRIAVAGLNPHAGENGLFGDEEIREIVPGIQAAREEGYEATGPWPPDTVFARAMAGEFDAVIAMYHDQGHIPIKLAGSPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J47</accession>
    <name>Q67J47_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3322"/>
    <dbReference type="EnsemblBacteria" id="BAD42303">
        <property type="protein sequence ID" value="BAD42303"/>
        <property type="gene ID" value="STH3322"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3322"/>
    <dbReference type="eggNOG" id="COG4481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180138_1_1_9"/>
    <dbReference type="OMA" id="RIKCLGC"/>
    <dbReference type="OrthoDB" id="2041238at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009296">
        <property type="entry name" value="DUF951"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38455">
        <property type="entry name" value="PTHR38455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06107">
        <property type="entry name" value="DUF951"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037263">
        <property type="entry name" value="DUF951_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8764" checksum="9E33EAE134775EDE" modified="2004-10-11" version="1">MERVRYEIGDQVRLKKPHPCGSNRWEIYRTGVDFGLKCLGCGHRVMIPRPKFEKAVRERFPKDQLAQEQQQPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD5</accession>
    <name>Q67JD5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stage V sporulation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoVB</name>
        <name evidence="8" type="ordered locus">STH3233</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3233"/>
    <dbReference type="EnsemblBacteria" id="BAD42215">
        <property type="protein sequence ID" value="BAD42215"/>
        <property type="gene ID" value="STH3233"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3233"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_2_1_9"/>
    <dbReference type="OMA" id="HYFDAPL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13124">
        <property type="entry name" value="MATE_SpoVB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024923">
        <property type="entry name" value="PG_synth_SpoVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038958">
        <property type="entry name" value="PG_synth_SpoVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="385"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="549" mass="57197" checksum="2B6B96F455A7E6BC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ0</accession>
    <name>Q67JJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Class-V aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3178</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3178"/>
    <dbReference type="EnsemblBacteria" id="BAD42160">
        <property type="protein sequence ID" value="BAD42160"/>
        <property type="gene ID" value="STH3178"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3178"/>
    <dbReference type="eggNOG" id="COG0075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_0_0_9"/>
    <dbReference type="OMA" id="IRINHMG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="7">
        <location>
            <begin position="37"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000524-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="396" mass="42975" checksum="982505721C026080" modified="2004-10-11" version="1">MERRFGELNPPQRLLMGPGPSNVDPRILRAMATPVIGHLDPAFLSIMDETMGWLREVYETRNHHTVPISATGSAGVEAMMVNLLEPGDDVVVGIIGYFGQRMADVASRTGANVRVIEAPLGEAIPPERFEEELRRKPAKLVALVHAETSTGAEQEIAPVAEIAHRYGALIAIDCVTSLGGMPVRVDEWGVDAAASCSQKCLGAPPGLSPVTFSPRAMEVITGRKSKCQSWYLDVNLLFAYWGDGKANNRVFHHTGPITMIYAMREALRIALEEGLEARYARHRKAHEAFAAGLEAMGLTLFTKGKRLPMLNVINIPEGIDDLAVRRRLLEQNIEISGGFGPLAGRVWRVGLMGVNANPAPVITLLGALEQALVEQGYRCERGAAVAAARAVYKQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL6</accession>
    <name>Q67JL6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome C oxidase mono-heme subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3152</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197265.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3152"/>
    <dbReference type="EnsemblBacteria" id="BAD42134">
        <property type="protein sequence ID" value="BAD42134"/>
        <property type="gene ID" value="STH3152"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3152"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2993">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050647_0_0_9"/>
    <dbReference type="OrthoDB" id="1469969at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003468">
        <property type="entry name" value="Cyt_c_oxidase_monohaem-su/FixO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02433">
        <property type="entry name" value="FixO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="59"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="223"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="320" mass="35373" checksum="728BAA82B2AD7661" modified="2004-10-11" version="1">MGNKFERNAALIGLGAFLLLTFAVIITVAVPLMQEDLYAASPSSPRYSGMFPDGKPISPQVARGREIYIREGCFYCHTQQVRTLPNDAAFQAPAVTLDDGRVVGGRPTRPEDYGNDNPALLGSQRTGPDLKYVGHRLPSKEWHIAHLIDPRSTEPNSIMPSYAHLPPDELDALAEYLLVLRDWAVPVQTLAKYPGPNILEATDDILPDEYRDLQNPYSIDDAAVLARAQELIEANGCLQCHGADMRGMTAADGWGSPPYPTNWFEAGKVHSEQFIFWVISEGTLKEDGSGSGMPSWRLNGLSDEDRWALTTYIKWLGTQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP1</accession>
    <name>DISA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA integrity scanning protein DisA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cyclic di-AMP synthase</fullName>
            <shortName evidence="1">c-di-AMP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Diadenylate cyclase</fullName>
            <ecNumber evidence="1">2.7.7.85</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">disA</name>
        <name type="ordered locus">STH3127</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in a DNA-damage check-point that is active prior to asymmetric division when DNA is damaged. DisA forms globular foci that rapidly scan along the chromosomes during sporulation, searching for lesions. When a lesion is present, DisA pauses at the lesion site. This triggers a cellular response that culminates in a temporary block in sporulation initiation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Has also diadenylate cyclase activity, catalyzing the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP acts as a signaling molecule that couples DNA integrity with progression of sporulation. The rise in c-di-AMP level generated by DisA while scanning the chromosome, operates as a positive signal that advances sporulation; upon encountering a lesion, the DisA focus arrests at the damaged site and halts c-di-AMP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DisA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197240.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JP1"/>
    <dbReference type="SMR" id="Q67JP1"/>
    <dbReference type="STRING" id="292459.STH3127"/>
    <dbReference type="EnsemblBacteria" id="BAD42109">
        <property type="protein sequence ID" value="BAD42109"/>
        <property type="gene ID" value="STH3127"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3127"/>
    <dbReference type="eggNOG" id="COG1623">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787128_0_0_9"/>
    <dbReference type="OMA" id="VVRDYVP"/>
    <dbReference type="OrthoDB" id="1139866at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01438">
        <property type="entry name" value="DisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038331">
        <property type="entry name" value="DisA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018906">
        <property type="entry name" value="DNA_integrity_scan_DisA_link"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023763">
        <property type="entry name" value="DNA_integrity_scanning_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10635">
        <property type="entry name" value="DisA-linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000255652" description="DNA integrity scanning protein DisA">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="DAC" evidence="2">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01130"/>
        </source>
    </evidence>
    <sequence length="351" mass="39074" checksum="ABCA99FAD21B9F3E" modified="2004-10-11" version="1">MEDRSGFWQVLQQLAPGTALREGLESILLARTGSLIVVGDSPEVLALVDGGFRLDCEFTPTRLYELAKMDGAIILTRDAQRILYANAMLVPDPSIPTSETGTRHRTAERVARQTGELVISISQRRDLITLYKGPMKYILRDFAVVLTKANQALQTLEKYKLVRDQAVARLSALELDDMVSALDVALVVQRTEMLLRIGKEIERYIVELGTEGRLVAMQLEELLAGVAAEGLLIIRDYASTDDPQHAAALRQQMAEWTYEELQDLAAVARLLGAGALDSPLGARGYRMLRRIPRLPAAVIENLVARFGRLQRVLAASLEELDDVEGIGEVRARAIQEGLRRMRMQFALERQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS8</accession>
    <name>RL11_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplK</name>
        <name type="ordered locus">STH3090</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197205.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JS8"/>
    <dbReference type="SMR" id="Q67JS8"/>
    <dbReference type="STRING" id="292459.STH3090"/>
    <dbReference type="EnsemblBacteria" id="BAD42072">
        <property type="protein sequence ID" value="BAD42072"/>
        <property type="gene ID" value="STH3090"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3090"/>
    <dbReference type="eggNOG" id="COG0080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_2_1_9"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="1702697at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104392" description="50S ribosomal protein L11">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <sequence length="140" mass="14924" checksum="E4EFA0A475909277" modified="2004-10-11" version="1">MAKKVTAVIKLALPAGKATPAPPVGPALGAHGVNIMAFCKEYNERTKDQVGLVIPVEITVYEDRSFTFILKTPPTAVLIKKALGIETASGVPNKQKVGKLTDAQVEEIAKIKMPDLNANDLEAAKRMVRGTARSMGVDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT0</accession>
    <name>Q67JT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3088"/>
    <dbReference type="EnsemblBacteria" id="BAD42070">
        <property type="protein sequence ID" value="BAD42070"/>
        <property type="gene ID" value="STH3088"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3088"/>
    <dbReference type="HOGENOM" id="CLU_3222988_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="44" mass="5436" checksum="771F2ECC965F5909" modified="2004-10-11" version="1">MDERQIRQRLESLVDRWLKASPAEKRILIQEKIRLEEMLEEERA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU8</accession>
    <name>RL22_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplV</name>
        <name type="ordered locus">STH3070</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197185.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU8"/>
    <dbReference type="SMR" id="Q67JU8"/>
    <dbReference type="STRING" id="292459.STH3070"/>
    <dbReference type="EnsemblBacteria" id="BAD42052">
        <property type="protein sequence ID" value="BAD42052"/>
        <property type="gene ID" value="STH3070"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3070"/>
    <dbReference type="eggNOG" id="COG0091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_3_3_9"/>
    <dbReference type="OMA" id="KRIQPRA"/>
    <dbReference type="OrthoDB" id="1666043at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243215" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="113" mass="12645" checksum="462B2443690D5DA2" modified="2004-10-11" version="1">MEVKASARFVRIAPRKVRVVIDLVRGKSVNEALALLKFIPKRASEPVAKVIASAAANAEHNFSLNKDNLYIAKAYVDQGPTLKRYHPRQRGQAFPILKRTSHITVVVKEKEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW5</accession>
    <name>Q67JW5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal protein L14E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3053"/>
    <dbReference type="EnsemblBacteria" id="BAD42035">
        <property type="protein sequence ID" value="BAD42035"/>
        <property type="gene ID" value="STH3053"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3053"/>
    <dbReference type="eggNOG" id="COG2163">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168121_1_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="RPL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="85" mass="9609" checksum="E0059AEDE888284C" modified="2004-10-11" version="1">MRSLAGRDLGTYYVVVRVLDERFVAVANGQRRTVSRPKVKNRRHLEVIGWVDASLALRLERGAKVTDEQIAKALETMPARVYEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY7</accession>
    <name>Q67JY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197145.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3030"/>
    <dbReference type="EnsemblBacteria" id="BAD42013">
        <property type="protein sequence ID" value="BAD42013"/>
        <property type="gene ID" value="STH3030"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3030"/>
    <dbReference type="eggNOG" id="COG1738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113622_0_0_9"/>
    <dbReference type="OMA" id="SWFINRN"/>
    <dbReference type="OrthoDB" id="1457141at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990397">
        <property type="term" value="P:queuosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003744">
        <property type="entry name" value="YhhQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02592">
        <property type="entry name" value="Vut_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="18321" checksum="A97C517FECF1BDF0" modified="2004-10-11" version="1">MLVALYLAAIVAANLSVVYFGPYASIINSFVFIGLDITTRDALHERWEGRNLWGKMALLILSGSVLSWLLNRDAGRIALASFTAFAASGAADTVVYHLLRHRPKLWRVNGSNAVSAAVDSVVFTSVAFGSFMPLIMAGQFLAKVAGGYLWALVLNRVYWRRAQAQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K12</accession>
    <name>Q67K12_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Flagellar basal-body rod protein FlgC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flgC</name>
        <name evidence="8" type="ordered locus">STH3005</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4 5">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197120.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3005"/>
    <dbReference type="EnsemblBacteria" id="BAD41988">
        <property type="protein sequence ID" value="BAD41988"/>
        <property type="gene ID" value="STH3005"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3005"/>
    <dbReference type="eggNOG" id="COG1558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123272_0_0_9"/>
    <dbReference type="OMA" id="YVAYPNI"/>
    <dbReference type="OrthoDB" id="1870921at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006299">
        <property type="entry name" value="FlgC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01395">
        <property type="entry name" value="FlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="7">
        <location>
            <begin position="96"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="16319" checksum="89F32E7BBB83F7AD" modified="2004-10-11" version="1">MRMFQAMEASASALTAERLRMDVIANNLANYRTTRTAEGGPYRRRTVVVEERPNTFASELARQAGRPAEPGLGVRVTQIVEDPSPFKEVHDPTHPDADPVTGIVRLPNVDLLQEITDLMLAQRMYDANLTAFNAAKNMALRALEIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K46</accession>
    <name>Q67K46_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ATP-dependent DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197084.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2969"/>
    <dbReference type="EnsemblBacteria" id="BAD41952">
        <property type="protein sequence ID" value="BAD41952"/>
        <property type="gene ID" value="STH2969"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2969"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025997_0_0_9"/>
    <dbReference type="OMA" id="FWLDAGS"/>
    <dbReference type="OrthoDB" id="207660at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="520"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="679" mass="73197" checksum="AEB4C1FF95976101" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K86</accession>
    <name>Q67K86_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197045.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2929"/>
    <dbReference type="EnsemblBacteria" id="BAD41912">
        <property type="protein sequence ID" value="BAD41912"/>
        <property type="gene ID" value="STH2929"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2929"/>
    <dbReference type="HOGENOM" id="CLU_2262414_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="103" mass="11813" checksum="8D5C0AD0BB9EE6BC" modified="2004-10-11" version="1">MSAGKGSEAGFLRQLAEAIRHHGGPAYDAQRARSLAEAVWRDLGHRAPDDFPHLVQEGDLRFVCQWYEQPPIMTVMVWDGEMWRDMERLLLPGEPPEREGETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB5</accession>
    <name>Q67KB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41883">
        <property type="protein sequence ID" value="BAD41883"/>
        <property type="gene ID" value="STH2900"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2900"/>
    <dbReference type="HOGENOM" id="CLU_1179726_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="25799" checksum="4E4879A86977763E" modified="2004-10-11" version="1">MLRRDRRSHRPVFLVLRSRPGDSGARPLAAPFASPDITVGPDGRPRAVVLNLGSREVVASTEFYSVPAGLPVTAENARLVGTGNPAIIRPGEAVTVSCNEPWLNRQADVLVVMAFHPELDPVARPFDVLADRHVGQMNYAWVGTYAGSLPDGDVRVEIRPAPQGLFRLKLYQEGALYPRCDRIMKPHGHRFHWMEVEGDLRMLFDLAVVDNNRLTLGVGPRGGSPRSGLLTRVTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF7</accession>
    <name>PURQ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">STH2856</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196975.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KF7"/>
    <dbReference type="SMR" id="Q67KF7"/>
    <dbReference type="STRING" id="292459.STH2856"/>
    <dbReference type="EnsemblBacteria" id="BAD41841">
        <property type="protein sequence ID" value="BAD41841"/>
        <property type="gene ID" value="STH2856"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2856"/>
    <dbReference type="eggNOG" id="COG0047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_9"/>
    <dbReference type="OMA" id="LHFVCRD"/>
    <dbReference type="OrthoDB" id="1527083at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100593" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <sequence length="235" mass="25288" checksum="B88F918125CA602A" modified="2004-10-11" version="1">MRFGILVFPGTNCEMETFYVLREVVGVQADYVWHEARDLTPYDAVVIPGGFTYGDRVRSGALACRAPVMEAVAEFAARGGLVLGICNGFQILTEAGLLPGGFRPNAHGRYRCGWSRVRVENAATPFTLACRPGQVLKIPVSHGMGNYQADPDTLRALSENQQVLFRYCTPEGAVTPGANPNGSAENIAGIVNRTGNVAGVMPHPERATEQVLGSADGRLLFASMVQHLTGRVIRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH5</accession>
    <name>HIS1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="1">ATP-PRT</shortName>
            <shortName evidence="1">ATP-PRTase</shortName>
            <ecNumber evidence="1">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisG</name>
        <name type="ordered locus">STH2838</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>Lacks the C-terminal regulatory region which is replaced by HisZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP phosphoribosyltransferase family. Short subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196957.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KH5"/>
    <dbReference type="SMR" id="Q67KH5"/>
    <dbReference type="STRING" id="292459.STH2838"/>
    <dbReference type="EnsemblBacteria" id="BAD41823">
        <property type="protein sequence ID" value="BAD41823"/>
        <property type="gene ID" value="STH2838"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2838"/>
    <dbReference type="eggNOG" id="COG0040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_2_0_9"/>
    <dbReference type="OMA" id="YVMMDYD"/>
    <dbReference type="OrthoDB" id="1419568at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13595">
        <property type="entry name" value="PBP2_HisGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01018">
        <property type="entry name" value="HisG_Short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024893">
        <property type="entry name" value="ATP_PRibTrfase_HisG_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000229334" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01018"/>
        </source>
    </evidence>
    <sequence length="221" mass="24515" checksum="A48D89E5F55719D0" modified="2004-10-11" version="1">MSPITIALPKGRPYAATVRFLREAGLAGPELEPEEGRSLYVECPAQGTRFIIARDSDVPTYVEYGAADLGIVGKNVLMELEPQVYELLDLGYSQCRFVLAAPAGVDPRQLLSGRSRQRVATKYPRMTEAYFNSRGLQVETIFLHGSIEVAPKVGLADLIVDIVETGRTLRENNLVVVEELWTSSMRLIANRAAYRLRAERIGPMVQRLRELVSRRAVAANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL6</accession>
    <name>Q67KL6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysophospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196916.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2797"/>
    <dbReference type="ESTHER" id="symth-q67kl6">
        <property type="family name" value="Monoglyceridelipase_lysophospholip"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41782">
        <property type="protein sequence ID" value="BAD41782"/>
        <property type="gene ID" value="STH2797"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2797"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_7_2_9"/>
    <dbReference type="OMA" id="FHGFSDH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="31311" checksum="16ED7A7CAEF0C24A" modified="2004-10-11" version="1">MRERSGTLSGLGGLKLYYRCWEPEHVQGNLVLVHGAGEHVGRYEHVAAWFAGRGFAVWAMDHRGHGRSEGTRMHVDRFSDYLVDLAAFVKLAAEAHGRPVMIGHSMGGLIAYRYAAAHPETISALVLSSPWFLSRAKVSRLEQALAPVLAVISPRLQVKSGIPPEICTRDAERIALDQKDPLRCQTATPRWFVECTRAAAECRTRVAFPEGLPALFLVAGTDHLVDPEATRAVFDRIGHGDKRFKLYPEKYHEIFNDPGREEVFAEILDWLRAHGLAPQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM5</accession>
    <name>Q67KM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2788"/>
    <dbReference type="EnsemblBacteria" id="BAD41773">
        <property type="protein sequence ID" value="BAD41773"/>
        <property type="gene ID" value="STH2788"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2788"/>
    <dbReference type="eggNOG" id="COG1413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1124073_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="26332" checksum="652D881900545ECA" modified="2004-10-11" version="1">MKGGRAMEALLAQILVGLASPDGERRVQAARELEALLSARLDEGETEWTPVIDALLPSMVAGLGDPEKGVQVHCANCLEFLAYQSGAVVPALRAALKPPDGRQAWTAAFVLARLGLWSDEVGEALSAAMGAPDRDTRWAAAGFALGLGRAHPECVAMVRRTLRAPVPNARKMAAYCLGAMGEYADVAADLADRLADPDRDVRRAAVLALNRLPHVDAAIRQAVARLRQDPDEFVRRAADAVAARWGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT2</accession>
    <name>Q67KT2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2154 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196849.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2730"/>
    <dbReference type="EnsemblBacteria" id="BAD41715">
        <property type="protein sequence ID" value="BAD41715"/>
        <property type="gene ID" value="STH2730"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2730"/>
    <dbReference type="eggNOG" id="COG4758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1065297_0_0_9"/>
    <dbReference type="OrthoDB" id="1687355at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016975">
        <property type="entry name" value="Cell_wall-act_antibiotic_resp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024425">
        <property type="entry name" value="DUF2154"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09922">
        <property type="entry name" value="DUF2154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031509">
        <property type="entry name" value="Cell_wall_LiaF/YvqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2154" evidence="2">
        <location>
            <begin position="136"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="261" mass="27929" checksum="B1C06903E3E5ADA9" modified="2004-10-11" version="1">MRRNTIGLLLVIVGALYLLQTMGWIRGISFGAAALLLIGLSIAGSGLRRSRPRVFTLGVGLWIAAIGLFNILAQAGVTTITGGDVLRMGWPLLLIALGISMLFGRGVGVYVFTTPRSSGQSSRSGSYKPVRSGTHFVSDLKMGNEPWVLEEDLHLQTFVGDLRLDLTTAAISPGVHRIEIAKFVGDTKVRVPDNVSVRARAEATITGSLDVLGEERDGVGAVSLEREEIVPGADAELIIDVHVGVGDITIERVPTTQFRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU5</accession>
    <name>Q67KU5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sugar transferase involved in lipopolysaccharide synthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2716</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2716"/>
    <dbReference type="EnsemblBacteria" id="BAD41701">
        <property type="protein sequence ID" value="BAD41701"/>
        <property type="gene ID" value="STH2716"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2716"/>
    <dbReference type="eggNOG" id="COG1086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_0_0_9"/>
    <dbReference type="OMA" id="LETRIMK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017475">
        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03025">
        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="8">
        <location>
            <begin position="247"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="458" mass="50646" checksum="58BC6C3E7B8759FB" modified="2004-10-11" version="1">MPGQGGSWLWGALVDIMLLVAALAGAMVWRVGWPPPETNLQSLLVVAPITFMALPFVFFGFGLYLRKPARSIVRSALFAESALFLVALAAAFWFRGFSSPRSVLVVGFLIHFIAVIGWRWGLQKTKHARASRVTVVATRDEAVAVVEKLLYEPSGWYEIVRVIEPGQLLAEGDVALVGSDMVVAGPSIVGRTRLVLLETAVRAGARVFIMPELSDILMVGSVAGQIGDAPVFEIRPLMLTGRQLVGKRVLDLSLSIPLLIMLLPVMAAVALAIKLFSPGPVFFTQKRVGLNGKEFVLYKFRTMVVDAEKRTGPVLATANDPRITPIGEFLRATRLDELPQLINVVRGEMSLVGPRPERPYFVDQFCRELPDYDLRHLTPPGITGLAQVMGRYYTSAQDKLRFDLYYIRHYSIWLDLKILLLTLQAVISRESATGVEASPTPQRMRAERLVLDRSGRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ4</accession>
    <name>Q67KZ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="2">PPIase</shortName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2667</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2667"/>
    <dbReference type="EnsemblBacteria" id="BAD41652">
        <property type="protein sequence ID" value="BAD41652"/>
        <property type="gene ID" value="STH2667"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2667"/>
    <dbReference type="eggNOG" id="COG0652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_16_3_9"/>
    <dbReference type="OMA" id="MRAPIVN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="4">
        <location>
            <begin position="46"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="21944" checksum="0049B0607F648109" modified="2004-10-11" version="1">MYNNRSPAGQQGGAGQGGADQAGHKQWDKPPEMIIDPAKRYTATMETSLGTMTFELFADEVPQTVNNFVFLAREGFYDGVTFHRIIKDFMIQGGDPTGTGTGGPGYTIPDEYPVTRPYQRGTLAMARTTAPNSAGSQFFICTGLSCGGLDQQPVYVIFGQLIEGDDVLTQLESVEVRQGNSFDPVPSTPVNPPVIRKVTIAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L14</accession>
    <name>Q67L14_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Membrane transporter similar to multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2647</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2647"/>
    <dbReference type="EnsemblBacteria" id="BAD41632">
        <property type="protein sequence ID" value="BAD41632"/>
        <property type="gene ID" value="STH2647"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2647"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_6_9"/>
    <dbReference type="OMA" id="AIGPPIF"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="10">
        <location>
            <begin position="38"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="431"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="473" mass="49474" checksum="3E45B182844E264B" modified="2004-10-11" version="1">MSQARRSGTINVIVSGRDVPVDQSNAPAAQAVTNLWLQLAALCTVPLIMVLGNSMLIPVLPDMGRVMGLSKATTGLAVTAFSLSAGIAIAVAGFLADRYGRKLVVVPSLLVYGLGGIVSGLAGWRMGPNGFAVVMAGRVIQGIGAAGTAPVAMAFVGDLFRGGSRVTALGIFEASNGLGKVISPILGALIGQVLVWWAVFFAYAVLAVPFALAVWFLTREPPRRKAPPAREYFGQVLTIFRRKGVALIACFWAGTVALFVLFGVLLFLSEHLETVHGIDGVPKGVRIMWPVLAMSATSFAAGLFLQRRGQLLKPAVVLGLALAIAGTVLIAFVRADWAFYAGLVVAGIGTGLQLPGLNNLITSSARTEQRAMITSVYGAVRFIGVAFGPPLYGALMRNGEALPFWFSAGVLATSLAATLFLIRPERLVEAEDEAGAEAQGPDRREGGGGRGEVAIPAWEPPANLPLGTRAPLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L16</accession>
    <name>Q67L16_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2645"/>
    <dbReference type="EnsemblBacteria" id="BAD41630">
        <property type="protein sequence ID" value="BAD41630"/>
        <property type="gene ID" value="STH2645"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2645"/>
    <dbReference type="HOGENOM" id="CLU_946375_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027783">
        <property type="entry name" value="Bacterial_PH-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10882">
        <property type="entry name" value="bPH_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_5" evidence="2">
        <location>
            <begin position="48"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="bPH_5" evidence="2">
        <location>
            <begin position="192"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="32342" checksum="326BA0F50789AC0C" modified="2004-10-11" version="1">MIAMMNETFYPPRPARSQGLKGISFVTLAGLTLAVLALASSMLSALNMRYEVTPEALRIRSGFSTREIPLDEVTGVWRPEALTGGVRKFGTSVPNLRTGRWRFNETGDITLYATRLETLVVVDTADGRYGVTPENPDAFTDALRSRLPAGFSPAGGSGTAAASMLIPLLLVAVAVPTTLYAVGLPSRFPQTLRYELGPDGLTIRTGFRPVQVRYADVERVEVASPKGYPIRIYGTAVGSLLWGKFRWPDAGPNLYLYCTRMKPLVLLRLRDDRTIGITPEEDERFVAELRKRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L39</accession>
    <name>Q67L39_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2622</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2622"/>
    <dbReference type="EnsemblBacteria" id="BAD41607">
        <property type="protein sequence ID" value="BAD41607"/>
        <property type="gene ID" value="STH2622"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2622"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_1_9"/>
    <dbReference type="OMA" id="SVGPYRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="60"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="268" mass="29604" checksum="B725EFE13EF410E9" modified="2004-10-11" version="1">MRSTRWRRRPRSRPRSSSRSEDARGPQAARGTAVWGLFEGRSAMRWDVIPTFVPIIFEGVKITLQISALGILFGTLIGLVAGLLRSRKPSNLILWALYGLATLYVEVVRGTPFLVQLYLMYYGPFFLFGIDLPAMTAGTIALSLNSGAYVAEIFRGAIESIDRGQMEAGRSLGLTFAQTMRHIILPQAFRRALPPLANEFASLIKESSVISVLGVADIMFKTKTVGTAHFAMFQALLVAAVYYLVLTGITSQLVRLLERRLGKSDSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L53</accession>
    <name>Q67L53_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196730.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2608"/>
    <dbReference type="EnsemblBacteria" id="BAD41593">
        <property type="protein sequence ID" value="BAD41593"/>
        <property type="gene ID" value="STH2608"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2608"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046841_0_0_9"/>
    <dbReference type="OMA" id="RTKGFWI"/>
    <dbReference type="OrthoDB" id="1155696at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="407" mass="43623" checksum="63ADD3D0204E8A46" modified="2004-10-11" version="1">MRNLWLVTRREFTSRIKSGAFIASTLIMMIVVFAATAIPLLMGGSSAPLSVVVLDRTGQLLAPLQQAVAAMEGGREVVLTPGEGDERTLMAEARAQGSVLLILDGTYPDAVHARLLYNSMGDLSDSYLVTDPLVQLVRAARLEQRGLPQELAQEVLQPLTLETLQLRSAEEGASDQFLGTLFLALGAIMSVYMISMMNSQFVFQGVLEEKVSRVVEVMAAAVRPSEMMLGKILGLGLLGILQYIGMMAAWLAGSALTMRAIGASMAGLTPGVALLIAAFVVLSYAMNASVLAALGATVSRMEDSQTMVSPVLILMMLPMFLLTPVMNDPNGTLATALSFVPLWTPIVMLMRVMMADVPAVQVAVSLALLAATTALLWWASGRIYRAALLSFGARPTLKQLWQYLRAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-09-13" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD9</accession>
    <name>PXPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-oxoprolinase subunit A</fullName>
            <shortName evidence="1">5-OPase subunit A</shortName>
            <ecNumber evidence="1">3.5.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-oxoprolinase (ATP-hydrolyzing) subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pxpA</name>
        <name type="ordered locus">STH2522</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.5.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex composed of PxpA, PxpB and PxpC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LamB/PxpA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LD9"/>
    <dbReference type="SMR" id="Q67LD9"/>
    <dbReference type="STRING" id="292459.STH2522"/>
    <dbReference type="EnsemblBacteria" id="BAD41507">
        <property type="protein sequence ID" value="BAD41507"/>
        <property type="gene ID" value="STH2522"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2522"/>
    <dbReference type="eggNOG" id="COG1540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069535_0_0_9"/>
    <dbReference type="OMA" id="DRTYKQD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00691">
        <property type="entry name" value="PxpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="PTHR30292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000185054" description="5-oxoprolinase subunit A">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00691"/>
        </source>
    </evidence>
    <sequence length="261" mass="27383" checksum="4BCA073C07E8CF22" modified="2004-10-11" version="1">MGAIRGPVRKIDLNCDMGESFGVYRIGADEEIMPLITSANIACGFHGGDPQVMRRTVRLAREHGVAVGAHPGYRDLVGFWRRPVRCSPDEVYADVLYQIGALAAFCRAEGVALRHVKPHGALYNTAAADAAIAGAVARAVADFDRSLMLYAPPGSALEQAGLAAGLRVIREGFADRGYAADGTLLPRTHPGAVLHEPERAAAQARGMVCSGTVTADTGEEVAVPAETLCVHGDHPSVIQVLRRIRSELEAAGVSVGAPGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF8</accession>
    <name>Q67LF8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metalloendopeptidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2503"/>
    <dbReference type="EnsemblBacteria" id="BAD41488">
        <property type="protein sequence ID" value="BAD41488"/>
        <property type="gene ID" value="STH2503"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2503"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_13_2_9"/>
    <dbReference type="OMA" id="YHLHYEI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269423" evidence="1">
        <location>
            <begin position="32"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="2">
        <location>
            <begin position="87"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="20536" checksum="C0ABEC13A4F282D0" modified="2004-10-11" version="1">MPLVHRRRWLLALVMVLTFPVAVWAALPAGAADPAQDVEEIARWIRERQDGAAPAPLLPCAWPAPGHAEVTSPFGYRLHPVLKRFRLHAGLDIGAPEGAAAAACWDGVVIAVAELPAYGRVVVLDHGGGLATVYAHLSAVRVSEGDRVPQGDEVGWVGVTGQVTGPHLHFEVWLDGRPVDPLRFADMAGLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK0</accession>
    <name>Q67LK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2461"/>
    <dbReference type="EnsemblBacteria" id="BAD41446">
        <property type="protein sequence ID" value="BAD41446"/>
        <property type="gene ID" value="STH2461"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2461"/>
    <dbReference type="HOGENOM" id="CLU_823677_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="337" mass="36025" checksum="D36750E347AE913F" modified="2004-10-11" version="1">MRNQDAAGAASWFSRIPGPHSLPVQRWVGVRDAGRVSRAGGRSGAKAAERTPRQRMCTFVESLRGRRFGARGAANAYGARASRKCTFASVVGFSAHVRTYWGPECRADQCRGNRCALSPSPYAAGISGLAGWEKPMGRGFHESAHSRQRRGRGQERNRGRGRAETRAGTTGTNVHLPRILTQQAFPGPRGGKSPWGAGFTKVHVRVNGRVQGECVNVSGPRASTGVVPRQRMCTFGESLRGRRFGARWPGEAYGTRVSRKCTFAPTSGARAGPSPGRGRTETRAGTTGTKVHSRVNGRPSGRVHERVRAPSFDGGSAEAKDPGRSGRGVGAVAQVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK1</accession>
    <name>Q67LK1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MutT/nudix family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2460"/>
    <dbReference type="EnsemblBacteria" id="BAD41445">
        <property type="protein sequence ID" value="BAD41445"/>
        <property type="gene ID" value="STH2460"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2460"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_18_2_9"/>
    <dbReference type="OMA" id="HHIMAFY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="17441" checksum="EA53A24C13066833" modified="2004-10-11" version="1">MTPPDEPALRETRIGAYGICICDDRILVVRKAKGPYKGLYDLPGGGVEFGESPTETVAREFLEEAGVAVEVKELVGSFSLVSVFPADSGTHLVELHHLGFLYRVNLAAPAPIKEDPDGRDSLGSVWLPLRDASPDKLSPLAREGLQCISSQGSGISLRRRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM7</accession>
    <name>Q67LM7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2434"/>
    <dbReference type="EnsemblBacteria" id="BAD41419">
        <property type="protein sequence ID" value="BAD41419"/>
        <property type="gene ID" value="STH2434"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2434"/>
    <dbReference type="HOGENOM" id="CLU_3066978_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="53" mass="5823" checksum="4131DA7F07D9AE25" modified="2004-10-11" version="1">MLPTVQEVLQADWQETAQSVQPANCTVVCSRGADRVRICLAKENPPIAWITPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW0</accession>
    <name>Q67LW0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41336">
        <property type="protein sequence ID" value="BAD41336"/>
        <property type="gene ID" value="STH2351"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2351"/>
    <dbReference type="HOGENOM" id="CLU_936663_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="31953" checksum="7A18C052E33196AF" modified="2004-10-11" version="1">MSRSGVRPPELPAAREGSAAVRDGRIAVTDPQGDGRPAVLIPGDDVILMIEGQRVTLPVAVFSWQDVQVRPRRPACPVRGFRVAVSPDGLSAYLEVEEQGPPGYRIADAGPARVLALTAEPLPGLQANPRDADVHQALRRAGVSAGVIPSAVAYALASPAARTVQVTWLPELPAARTVQVTWLPEVPAARTVQVTWFPEVPAARTVQVTWLPEVPAARTVQVTWLPEVPAARTVQVTWLPEVPAAHTVQVTWLPEVPAARTVQVAWLPEVPAARTVQVAWLPDVPAPPAVTRRRRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX8</accession>
    <name>Q67LX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2333"/>
    <dbReference type="EnsemblBacteria" id="BAD41318">
        <property type="protein sequence ID" value="BAD41318"/>
        <property type="gene ID" value="STH2333"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2333"/>
    <dbReference type="eggNOG" id="COG3381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077650_0_0_9"/>
    <dbReference type="OMA" id="EDHLGRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="SSF89155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="244" mass="27026" checksum="81903E48BD48A9B5" modified="2004-10-11" version="1">MGTKLVRAVTVLAVPRGRRELAVDLPDLAWYCEVRAEVYGLLARLFRDPPDERLLAVIRHPDFVREWPVGRGQPDVDRGLERLAAALPAVDPDALRHEFWHLFGTLGPAAAPPWQSVYLDREGALMGEETLRVRALYARFGLEAELAVAVTDDHIAVQLAFVSELSRRAAERLRTGDVAGARDLLAGQRECLAEHLLRWADRFATKVEAAGGTGFYAGLARLTLGLLRVDCKFLEEVAAEPVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY7</accession>
    <name>Q67LY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2324"/>
    <dbReference type="EnsemblBacteria" id="BAD41309">
        <property type="protein sequence ID" value="BAD41309"/>
        <property type="gene ID" value="STH2324"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2324"/>
    <dbReference type="eggNOG" id="COG2771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2511479_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="85" mass="9492" checksum="D33D7B51751C4F8C" modified="2004-10-11" version="1">MRSSGGTRRARKPNTFRCCGHQVEKGVIDPVGDRLYYAGRYMRHHVAEAHGSVFAYLIGLDKLQTSGERGDNLPRELEDDDDGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M78</accession>
    <name>Q67M78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196355.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2230"/>
    <dbReference type="EnsemblBacteria" id="BAD41215">
        <property type="protein sequence ID" value="BAD41215"/>
        <property type="gene ID" value="STH2230"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2230"/>
    <dbReference type="HOGENOM" id="CLU_1739601_0_0_9"/>
    <dbReference type="OrthoDB" id="1633799at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009936">
        <property type="entry name" value="DUF1468"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07331">
        <property type="entry name" value="TctB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="150" mass="16471" checksum="368B93879CAC8DF5" modified="2004-10-11" version="1">MKKHDRIGAVVLIVIGVAAAVYSVIKLKVGTLRVPGSGFMPLLASLAVIGGSIGWLWEVRGPDPDPRPLWPDKNWLRPLLGLLFLILYALLFKPLGHLFSTLVFLGLWQFLVERVNWRRATLVTLLGAAGMYLLFVVLLGVHVPTSPLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M79</accession>
    <name>Q67M79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196354.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2229"/>
    <dbReference type="EnsemblBacteria" id="BAD41214">
        <property type="protein sequence ID" value="BAD41214"/>
        <property type="gene ID" value="STH2229"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2229"/>
    <dbReference type="eggNOG" id="COG3333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022936_2_0_9"/>
    <dbReference type="OMA" id="AMEGNLM"/>
    <dbReference type="OrthoDB" id="735109at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="499" mass="51902" checksum="5081EF80AF84DCB9" modified="2004-10-11" version="1">MESLQGLMQGFSVAMLPTNLLFAFLGCLLGTAIGVLPGLGPAGTISLLLPVVYSMGSSATTLIFLAGIYYGSMYGGSTTSILLNLPGEAASVITAIDGYEMAKKGRAGAALSIAAIGSFLAGTLAVVGLTFVAPPVAEFALDFGPPEYFSLTLLGLFLAVFLSGGSMAKGVVLLAAGVLLASVGLDPVTGKARFTFGSVAMQSGFDFTTLAMGVFGLGEIFFNLEKADFMGTITAKIGRLLPTAKEWAESRWAIVRGAVLGFAVGVLPGGGGVLGSLASYATERKLSKHPEEFGKGAIAGVAGPESANNAASTASFIPLLTLGIPTNSSMAVIFAALLIQGVTPGPFLITEHPDVFWGVIASMYIGNLMLLVLNLPLVGVWVRLLKVPFNILGPLVVLLTVIGVYSTTNDVFQVFVLIGFGVLGYFMRKFRFDPGPLPLAFVLAPTIENSLRQSLIMSRGSAAIFFTRPISVTLLRIFAALVIGQLAKGLRSHRRTAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M84</accession>
    <name>Q67M84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2224</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196349.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2224"/>
    <dbReference type="EnsemblBacteria" id="BAD41209">
        <property type="protein sequence ID" value="BAD41209"/>
        <property type="gene ID" value="STH2224"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2224"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005532_0_0_9"/>
    <dbReference type="OMA" id="MYIPLFA"/>
    <dbReference type="OrthoDB" id="159395at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013496">
        <property type="entry name" value="CHP02680"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02680">
        <property type="entry name" value="TIGR02680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="380"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="480"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="774"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="918"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1401" mass="156561" checksum="6813697FE1E0B44A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG5</accession>
    <name>Q67MG5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0751 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2143"/>
    <dbReference type="EnsemblBacteria" id="BAD41128">
        <property type="protein sequence ID" value="BAD41128"/>
        <property type="gene ID" value="STH2143"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2143"/>
    <dbReference type="eggNOG" id="COG4378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151785_1_0_9"/>
    <dbReference type="OMA" id="CEYCKVF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016772">
        <property type="entry name" value="UCP020408"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10087">
        <property type="entry name" value="DUF2325"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020408">
        <property type="entry name" value="UCP020408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="11636" checksum="4459D850DC5DCC36" modified="2004-10-11" version="1">MVLVIGGDHLGSIDEHLRRLGYRSVKHVTGRSERRVEIPSGTELVVVLIDYVNHNLARWVKQQAKARGLPIIFSRRSWSSICASLRNCEDCPHRKGCLDSSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH5</accession>
    <name>Q67MH5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2133"/>
    <dbReference type="EnsemblBacteria" id="BAD41118">
        <property type="protein sequence ID" value="BAD41118"/>
        <property type="gene ID" value="STH2133"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2133"/>
    <dbReference type="eggNOG" id="COG1739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083552_2_1_9"/>
    <dbReference type="OMA" id="HCYAYTT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015796">
        <property type="entry name" value="Impact_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015269">
        <property type="entry name" value="UPF0029_Impact_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="PTHR16301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09186">
        <property type="entry name" value="DUF1949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00257">
        <property type="entry name" value="IMPACT_YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0029" evidence="2">
        <location>
            <begin position="37"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1949" evidence="3">
        <location>
            <begin position="155"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="24426" checksum="95F07CD3BE5F008F" modified="2004-10-11" version="1">MCGGHSSNCTLSTLNARGERVSYRTVAGRGQAEIVIKKSRFIAHVAPVASEEAAWAFINEIRAAHSDATHNCFAFTAGAVQRMSDDGEPSGTAGRPIFEVLEKQGLSDTAIVVTRYFGGILLGAGGLVRAYSQAAAAGVEAAGVAEALPAVDLRVRVDYALLGRVQYLLQQHGALTLQTDFGQEVVIVFRVLETDRDRVTAELAEASAGRIAVEPVGTALVGPDLQPIHRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML0</accession>
    <name>Q67ML0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome C oxidase subunit III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2098</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196223.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2098"/>
    <dbReference type="EnsemblBacteria" id="BAD41083">
        <property type="protein sequence ID" value="BAD41083"/>
        <property type="gene ID" value="STH2098"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2098"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_3_2_9"/>
    <dbReference type="OMA" id="FLDLLWV"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02863">
        <property type="entry name" value="Ubiquinol_oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033946">
        <property type="entry name" value="Ubiquinol_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="9">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="23439" checksum="513C765DBDDE3CF0" modified="2004-10-11" version="1">MAHHEHAHRAHPAWAHDAHAHQTNTGIDNRKLGFWLFIASESIFFACLLVGYLVFRGGYQPGVGPRDLFNIELTTVSTFVLLMSSLAMVLAVNAAQRNEGRAARRWVLVTALMGLTFLGFQAYEFTHFYHAGLSLSSSVFGTAFYTLTGFHGAHVAVGVIWLLTLLYHGRGKGLTVDKADDVEVAGLYWHFVDIVWIVLFTVVYLLEFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML3</accession>
    <name>Q67ML3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2095"/>
    <dbReference type="EnsemblBacteria" id="BAD41080">
        <property type="protein sequence ID" value="BAD41080"/>
        <property type="gene ID" value="STH2095"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2095"/>
    <dbReference type="eggNOG" id="COG5349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133146_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009325">
        <property type="entry name" value="DUF983"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06170">
        <property type="entry name" value="DUF983"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="13035" checksum="CC9624598F03C3FF" modified="2004-10-11" version="1">MLGPMARGAWDGFRLRCPACRRGAMSRGLFQLADRCPVCGAPFEPEEGDFVGAMLVAYSVTAVLVVIGIYVTAALTPLSPRTQLVLWCLFGAGFLIGTYRNMKGAWVGILHAMTGLRRGGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-01-04" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MN1</accession>
    <name>Y2077_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0316 protein STH2077</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH2077</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0316 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD41062" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41062.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_070105455.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MN1"/>
    <dbReference type="SMR" id="Q67MN1"/>
    <dbReference type="STRING" id="292459.STH2077"/>
    <dbReference type="EnsemblBacteria" id="BAD41062">
        <property type="protein sequence ID" value="BAD41062"/>
        <property type="gene ID" value="STH2077"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2077"/>
    <dbReference type="eggNOG" id="COG4843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106166_1_0_9"/>
    <dbReference type="OMA" id="KLALGYI"/>
    <dbReference type="OrthoDB" id="1480783at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01515">
        <property type="entry name" value="UPF0316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019264">
        <property type="entry name" value="DUF2179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044035">
        <property type="entry name" value="DUF5698"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022930">
        <property type="entry name" value="UPF0316"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40060">
        <property type="entry name" value="PTHR40060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10035">
        <property type="entry name" value="DUF2179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18955">
        <property type="entry name" value="DUF5698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000171960" description="UPF0316 protein STH2077">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="177" mass="19727" checksum="B21C6C18B23DD9B5" modified="2005-01-04" version="2">MRLAKEVEAALDLLIIFLAQATYVSVNTVRWIILVKGRRLLASAISFFEVILWVYALGLVVSQLSDPVKVATYALGYAVGALVGSKIEERLALGYVLFQVITTRIGELAPALREHGLGVTDWRAEGRMGQREVLMVVARRKNGPQVVRLLEELDPQAFVVQLDASWYRGGFIQKMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT4</accession>
    <name>Q67MT4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2024"/>
    <dbReference type="EnsemblBacteria" id="BAD41009">
        <property type="protein sequence ID" value="BAD41009"/>
        <property type="gene ID" value="STH2024"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2024"/>
    <dbReference type="HOGENOM" id="CLU_1546798_0_0_9"/>
    <dbReference type="OrthoDB" id="1731686at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="19308" checksum="FB22A1357D3E2996" modified="2004-10-11" version="1">MLTDETTVFATGQVRSVQTLAVPGVRFVPDRHQVQEAGFAYFAFLDRLARPLLRVRFGERGTAAVRLCGITLLSFRPPEVREAPGMASIRFPIAAGVLVQRPMRGRGELRFEMHADRLVMAVEGYYAALAGAGGSDVRHWIYERTQAAIHRRVAARYLDLWLGRLIAARSIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX8</accession>
    <name>Q67MX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40965">
        <property type="protein sequence ID" value="BAD40965"/>
        <property type="gene ID" value="STH1980"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1980"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_7_1_9"/>
    <dbReference type="OMA" id="IANCFEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="37953" checksum="86011E02EA3B0057" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQPNPVPECLLAGLGWVLCALRCQVSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N08</accession>
    <name>AROC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate synthase</fullName>
            <shortName evidence="1">CS</shortName>
            <ecNumber evidence="1">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroC</name>
        <name type="ordered locus">STH1950</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="1">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N08"/>
    <dbReference type="SMR" id="Q67N08"/>
    <dbReference type="STRING" id="292459.STH1950"/>
    <dbReference type="EnsemblBacteria" id="BAD40935">
        <property type="protein sequence ID" value="BAD40935"/>
        <property type="gene ID" value="STH1950"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1950"/>
    <dbReference type="eggNOG" id="COG0082">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_2_0_9"/>
    <dbReference type="OMA" id="MLSINAV"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140663" description="Chorismate synthase">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="254"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="314"/>
            <end position="318"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <sequence length="391" mass="41732" checksum="7A5D9CD2CE3B0BF5" modified="2004-10-11" version="1">MRYLTAGESHGRALVTIVEGLPAGVPVDLAAIDRDLARRQSGYGRGGRMKIEQDRVQVLSGIRHGKTLGSPVALLVENRDWLNWTEVMSPAPLEAYTDPRAAQKVRTRPRPGHADLAGALKYDHADLRNVLERASARETAARVAAGSLAKQYLAPFGIRVAGYVRSIGPVEAQPPAGLDLDGIVARAEASPVRCPDPAASARMVEEIDAAKRDGDSLGGVVEVVAAGLPPGLGSHVHWDRKLDGALGAALLSIQAAKGVEIGDGFLGARRRGSEVHDEIGWSPDRGYFRYTNRAGGLEGGMTNGMDLVVRVAFKPIATLYKPLRSVEIDTHVEAAAGIERSDVCAVPAAAVIAECVTAFELARFVAEKFGGDSLEEALRNFRGYLEQIARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N33</accession>
    <name>Q67N33_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonine-phosphate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.81</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-threonine-O-3-phosphate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH1925</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + O-phospho-L-threonine = (R)-1-aminopropan-2-yl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:11492"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58563"/>
            <dbReference type="ChEBI" id="CHEBI:58675"/>
            <dbReference type="EC" id="4.1.1.81"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.81" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1925"/>
    <dbReference type="EnsemblBacteria" id="BAD40910">
        <property type="protein sequence ID" value="BAD40910"/>
        <property type="gene ID" value="STH1925"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1925"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_2_9"/>
    <dbReference type="OMA" id="RDPWSVN"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005860">
        <property type="entry name" value="CobD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01140">
        <property type="entry name" value="L_thr_O3P_dcar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="10">
        <location>
            <begin position="15"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="339" mass="36848" checksum="F6CA9CC44E9293FF" modified="2004-10-11" version="1">MARARWGEPPGGFCDFSANINPLGPPAGAVAAAQAALAGIAHYPEPFARRLRAALARRHQVDESMVLVGNGAAEVLYLLLRLASGFRVALPEPGFAEYARAARAAGAELLPVAHDRTDPPPQLGRGDWWIICNPHNPTGHLFTPEQLLRAAERTEATLLIDEAFIDLTAAGEAGSVIARVPHRCNLVVVRSLTKFFALPGLRVGYAVAPRWMVAELDAARDPWSVNSLAQAAALAALSDEAYAARTRHWVAVERAYLAEQLASLPGFIVYPPSANFVLVRAPLPAHEIQRRLGPRGVLIRDCRSFAGLTEHHMRLAVRTRPENRRLIALLRSILEEEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG2</accession>
    <name>Q67NG2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195924.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1796"/>
    <dbReference type="EnsemblBacteria" id="BAD40781">
        <property type="protein sequence ID" value="BAD40781"/>
        <property type="gene ID" value="STH1796"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1796"/>
    <dbReference type="HOGENOM" id="CLU_1098066_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="25540" checksum="333E297114247A3E" modified="2004-10-11" version="1">MTEQEWQQHPEREVPALPGQEPHFPGGDPGGRPQGIHPGRSEGQAALSSGDSVPVLVAWFADASSARACLRALELRGVGVTLAEEAPPGHTGPVAIHASPSVGEKDPVMTERGMGRGAVLGATVGATAGFLAATYLVPPLGAASATGAMLTTLAGAGLGSFFGNLADQARDDSGNPAGQGGDAAAAQESATLRFRLEVSARPTQVDEVMALVSAWDPEEMRLLPTGAGEARGYPAGRRGDGDGGRIPVEGGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM9</accession>
    <name>Q67NM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1729</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195857.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1729"/>
    <dbReference type="EnsemblBacteria" id="BAD40714">
        <property type="protein sequence ID" value="BAD40714"/>
        <property type="gene ID" value="STH1729"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1729"/>
    <dbReference type="eggNOG" id="ENOG502ZNH6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076871_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024992">
        <property type="entry name" value="DUF3891"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13030">
        <property type="entry name" value="DUF3891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="262" mass="29361" checksum="C3A67879F7D41425" modified="2004-10-11" version="1">MIVRAECGQLCLTEQTEHARMAAELVGWWGGVCAPVEPADAVKLAVALHDAGWAEVDASPQVNPDTGRPYSFLDIPQDVHVAAHTRTVRRARQADPYAGLLTSLHSAGLYRNRYGHLPHIAQRPVAPQWREAVDRFLAEQDAVQEELTAALRPDPAVLWTHYRWFQLWDMLSLYSCMFSPGDTPDTLLCTVPRAPGGADITFYLQGSDAGFSVDPWPFTEDRIVLRWAARILPDRPYRIDAELQDAYARASVVQQEIVLHPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP6</accession>
    <name>Q67NP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195840.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1712"/>
    <dbReference type="EnsemblBacteria" id="BAD40697">
        <property type="protein sequence ID" value="BAD40697"/>
        <property type="gene ID" value="STH1712"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1712"/>
    <dbReference type="HOGENOM" id="CLU_1834163_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269914" evidence="2">
        <location>
            <begin position="29"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="14547" checksum="1F6DBE093CCDCA21" modified="2004-10-11" version="1">MPRWVRFLSCTVTGAVLSFGGVLLAAVAAERPPGFWRALQAFAVTPGFWALALLFCLLAAGAVAAARALVSLYGLPPAAAGALGGAVLATCYLAALVSRHLDAWGGWEGTLPRLWPAALWIALPFAASGAASGWLWERLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT3</accession>
    <name>Q67NT3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="ESTHER" id="symth-q67nt3">
        <property type="family name" value="5_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40660">
        <property type="protein sequence ID" value="BAD40660"/>
        <property type="gene ID" value="STH1675"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1675"/>
    <dbReference type="eggNOG" id="COG4099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064094_1_0_9"/>
    <dbReference type="OMA" id="ANHNSWD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="105"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="26283" checksum="81C8933EB6ED2736" modified="2004-10-11" version="1">MAPAELDRERVEPMQESRWFARTVQKQVCANYLLFLPPGYGDDRSRRWPLILFLHGAGERGSDLSLVKRHGLPKLLDERPGDFPFIVVSPQCPDGEVWDVDVLLALLDEVCETHAVDRDRIYVTGLSMGGMGTFALALAAPQRFAAAAPVCGWGNPLQVRPEHARVPFWVFHGELDDKVPAALSAQMVAALQAVGAPVRYTVYPGVGHDAWTPTYANAELYQWFLAHRRSGEPAAGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ6</accession>
    <name>Q67NZ6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Molybdenum cofactor biosynthesis protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in the biosynthesis of molybdopterin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195741.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1612"/>
    <dbReference type="EnsemblBacteria" id="BAD40597">
        <property type="protein sequence ID" value="BAD40597"/>
        <property type="gene ID" value="STH1612"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1612"/>
    <dbReference type="eggNOG" id="COG0521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_9"/>
    <dbReference type="OMA" id="TGWDGIL"/>
    <dbReference type="OrthoDB" id="1528139at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="PTHR43232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="20"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="18299" checksum="C342F5FFE9B2AD45" modified="2004-10-11" version="1">MGVHDHKEEAARAVGQLTAGIVTVSDTRTAETDESGQLLRELLTGAGHRVEAYAIVPDEVEAIRSAVVRFAERVDFVVVNGGTGMTPRDVTIEACRPLFSKELEGFGELFRMLSYQEIGSAAVMSRAAAGAVGRVMLFCLPGSKGAVRLATERLILPEIRHLVSHIRKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-02-09" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P10</accession>
    <name>NUON1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit N 1</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit N 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit N 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoN1</name>
        <name type="ordered locus">STH1598</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P10"/>
    <dbReference type="SMR" id="Q67P10"/>
    <dbReference type="STRING" id="292459.STH1598"/>
    <dbReference type="EnsemblBacteria" id="BAD40583">
        <property type="protein sequence ID" value="BAD40583"/>
        <property type="gene ID" value="STH1598"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1598"/>
    <dbReference type="eggNOG" id="COG1007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_5_9"/>
    <dbReference type="OMA" id="YYLQWTA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000391235" description="NADH-quinone oxidoreductase subunit N 1">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00445"/>
        </source>
    </evidence>
    <sequence length="491" mass="51193" checksum="8B802EC99F34B8EF" modified="2004-10-11" version="1">MPVDLNFTIAAPLLALAAGALLILLLDLLFRYETIQGPMYVAAVGAVLVAGWYLVPLWRGAAEPFGFHGMLVMDRFAAVYGLVLLGAALLAILLSFGRVREDQSGYLALLLWAAMGMVLLGGAGNLMVIFLGIELLSLALYVMIAFAPKRMAAREAAFKYFVLGSVAAAFLIFGFALIYGAAGTMSLTGIAAAARSFTSEGAWSVGLYYKVGVGLAIVGLAFKMALVPFHIWAPDVYQGAPTPVTAFMAIGTKAAAFAAMARLLVAAVPQAYQPSFLLPLSILAFASMMLGATVGIWQSDLKRLMAYSGIANAGYLIMAIPGLGLDGLSAAAYYLAAYGFATMGVFAVVRILEADGVDGSQLANLKGLFYRSPWVGVCLAVLFFGLIGVPPTGGFVGKFLLAIAAVRGGAWIVLTGLILSTGISAYVYLKVIGTAFTRTKVAPQPEEGEEPHPPTRTAAQVVLAIATAGTLVLGVLPGPVSELLRVALAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P34</accession>
    <name>Q67P34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1574</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195703.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1574"/>
    <dbReference type="EnsemblBacteria" id="BAD40559">
        <property type="protein sequence ID" value="BAD40559"/>
        <property type="gene ID" value="STH1574"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1574"/>
    <dbReference type="eggNOG" id="COG3185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012617_1_1_9"/>
    <dbReference type="OMA" id="ISYIIGY"/>
    <dbReference type="OrthoDB" id="361195at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="455" mass="49836" checksum="20516F0D5CCE9114" modified="2004-10-11" version="1">MRTVRIGAGQGFYGDSLLPVLDVARYGDVKYISFDTLAELTLAILEKGRRKDPTGGYTRDVVPMMRNLLPIAKEKGIRLITNAGGINPRGAARAVAEVARELGLSVRIACVTGDDIYDRLDELEARGVTFADKETGQALGDVRDRVLFASVYLGARVVADALATGADVVITGRTTDTAQFLGPLIHEFGWAPDDWDRLAQGIVLGHLMECSGQVTGGNYQVGWEDIPDLHRIGFPIAEVSEDGTFILTKAPGTGGRVDLKSVKEQFLYEIHDPTSYVTPDVVCDLTTTRLEQVGENRVRVSGTKGRPAPPTLKALLGYADGWMGEGYISFSWPKAYSKAKRAAQIIRARLEMQGVKPEEIHEEYIGINSLWGALAPEPVDEDQINEVRLRIAIRTRNKKDCEILAREFPPLLLSGPPTASAVAGTPQPRELMGLWSTLIPRELIEPYVKIEVEEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P69</accession>
    <name>CHED_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable chemoreceptor glutamine deamidase CheD</fullName>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheD</name>
        <name type="ordered locus">STH1539</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheD family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195669.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P69"/>
    <dbReference type="SMR" id="Q67P69"/>
    <dbReference type="STRING" id="292459.STH1539"/>
    <dbReference type="EnsemblBacteria" id="BAD40524">
        <property type="protein sequence ID" value="BAD40524"/>
        <property type="gene ID" value="STH1539"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1539"/>
    <dbReference type="eggNOG" id="COG1871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087854_2_0_9"/>
    <dbReference type="OMA" id="VGMADLN"/>
    <dbReference type="OrthoDB" id="1863047at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16352">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01440">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038592">
        <property type="entry name" value="CheD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005659">
        <property type="entry name" value="Chemorcpt_Glu_NH3ase_CheD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147">
        <property type="entry name" value="PTHR35147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03975">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="SSF64438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000251068" description="Probable chemoreceptor glutamine deamidase CheD">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01440"/>
        </source>
    </evidence>
    <sequence length="165" mass="17289" checksum="C22897E2CB23B071" modified="2004-10-11" version="1">MSDRRLEAVGLGELKVADRPDQVLVCYGLGSCVGLALYDPVVRIGAMVHVVLPDSSMGRGREAPPGKYADTGVEAAVAALVDAGASRSRLIAKAAGGARMLRLAGSNPQLDIGARNTEAVRAALARHQIRLVAEDMGGTYGRTLQLFIETGRVLVSTVGRGEHEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG5</accession>
    <name>RL32_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmF</name>
        <name type="ordered locus">STH1443</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PG5"/>
    <dbReference type="SMR" id="Q67PG5"/>
    <dbReference type="STRING" id="292459.STH1443"/>
    <dbReference type="EnsemblBacteria" id="BAD40428">
        <property type="protein sequence ID" value="BAD40428"/>
        <property type="gene ID" value="STH1443"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1443"/>
    <dbReference type="eggNOG" id="COG0333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129084_1_3_9"/>
    <dbReference type="OMA" id="HRACRHC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00340">
        <property type="entry name" value="Ribosomal_L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002677">
        <property type="entry name" value="Ribosomal_L32p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044957">
        <property type="entry name" value="Ribosomal_L32p_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35534">
        <property type="entry name" value="PTHR35534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01783">
        <property type="entry name" value="Ribosomal_L32p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01031">
        <property type="entry name" value="rpmF_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000225772" description="50S ribosomal protein L32">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="64" mass="7333" checksum="32E0EB9952816730" modified="2004-10-11" version="1">MAVPKRKVSKSRRDSRRAQTFRLEAPNLSPCPNCRTPRLPHRVCPNCGYYQGRVVIQHEAEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI4</accession>
    <name>Q67PI4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1424</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1424"/>
    <dbReference type="EnsemblBacteria" id="BAD40409">
        <property type="protein sequence ID" value="BAD40409"/>
        <property type="gene ID" value="STH1424"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1424"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_5_0_9"/>
    <dbReference type="OMA" id="SPWRDFL"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="103"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32910" checksum="4B72638B30090441" modified="2004-10-11" version="1">MRPVAAAREPDTRREDARTGRRVLRQALRQLWRRKVALLGVLMVLGAVICALFAPVLAPHDPNAQQVQLRLLPPFWMEGAHPAYPLGTDHLGRDILSRLIYGSRVSLTVGLSAVLIAGGIGTAAGLAAGYLGGAVDDLVGRLGDVQLAFPFTLLALVVMAVLGTGMGKTIAVLGITGWVFYARVLRSEVLALRERDFILASRALGNPGWRTVLRHLFPNVAGTLIVLATLEVPRVIITESALTFLGLGIQPPTVTWGGMLADARNYITTHWWVATFPGLALQLTVLGLNLTGDFLREILDPRLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP2</accession>
    <name>Q67PP2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycos_transf_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195496.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1366"/>
    <dbReference type="EnsemblBacteria" id="BAD40351">
        <property type="protein sequence ID" value="BAD40351"/>
        <property type="gene ID" value="STH1366"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1366"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068877_0_0_9"/>
    <dbReference type="OMA" id="ATWWETA"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="217"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="333" mass="36484" checksum="EEDBCC7FC7A3CFBF" modified="2004-10-11" version="1">MKITIPAFNLELGGGTRTLVRVAEGLAAYGHDVAFLIPERGGIQWKVTVPVISVPWIVPDAFPKSDIILTNFWSTVPPAWESGAGQVVRFSLGYEPLWVKDPRAKETYLLPIPTLAISQWLASRIEAVAGRRPQVIHLGIEQETFVPPPPDAPPRSGILYIWRDKTLGYGFKGTDDFVQALGPVMQRYPRLPLTIVTPVACRVHLPYPHRVVVAPTDAELAELYQQAAVFVSTSWFEGFSMPPLEAMSCGAAVVATNCGGIGEYARHQVNCLLVPPRTPMALAQAILTVLGDPALRQRLGAAGVATARDWSWQRTWKEMHEALTAIHEASRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP8</accession>
    <name>Q67PP8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alkaline-shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195490.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1360"/>
    <dbReference type="DNASU" id="2982593"/>
    <dbReference type="EnsemblBacteria" id="BAD40345">
        <property type="protein sequence ID" value="BAD40345"/>
        <property type="gene ID" value="STH1360"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1360"/>
    <dbReference type="eggNOG" id="COG1302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113198_2_0_9"/>
    <dbReference type="OMA" id="CYGLVGM"/>
    <dbReference type="OrthoDB" id="1961470at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34297">
        <property type="entry name" value="PTHR34297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="119" mass="12918" checksum="B59D7329B5D3F49C" modified="2004-10-11" version="1">MGKELITERGRIYVSNEVIATLAGIATTECYGIVGMASSRIQDGISELLGRENLARGVSVEVDGDSVEIDLYIVVGYGTRISEIGRNVTDQVRYVVESYTGLKVRAVRIHVRGVKLGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU4</accession>
    <name>Q67PU4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1314</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 130 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195445.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1314"/>
    <dbReference type="EnsemblBacteria" id="BAD40299">
        <property type="protein sequence ID" value="BAD40299"/>
        <property type="gene ID" value="STH1314"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1314"/>
    <dbReference type="eggNOG" id="COG2152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046648_0_0_9"/>
    <dbReference type="OMA" id="ARQEEPI"/>
    <dbReference type="OrthoDB" id="1622507at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="PTHR34106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016202">
        <property type="entry name" value="PH1107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="35238" checksum="429BE8F8D36CE288" modified="2004-10-11" version="1">MLKLQRLSQQPILTPVREHEWEREAVFNAGAIYENGLFWLFYRASNNRFRLDSPTPRPEEKFVSSIGLAVSTDGIHFSRFDRPVFAPATEQEAWGVEDPRITRIGDIYYMCYTAFGGRHWQDFRPALAWSRNLFEWHGREILLDEPNKDVALFPGKVNGRYVLLHRREPSIWIAFSDDLKSWTDHRILMEPIPGGWEAKKIGAAGPPHRTEAGWVLFYHAVDSDNVYRLGVALLDLADPTRVLARYPHPILEPETDWERNGLVPNVVFSCGSVEKDGAYFVYYGGGDCCLGVAAVDRTVLLHHLTPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX8</accession>
    <name>Q67PX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="6">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195411.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1280"/>
    <dbReference type="EnsemblBacteria" id="BAD40265">
        <property type="protein sequence ID" value="BAD40265"/>
        <property type="gene ID" value="STH1280"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1280"/>
    <dbReference type="eggNOG" id="COG1820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_2_1_9"/>
    <dbReference type="OMA" id="MFNHAPT"/>
    <dbReference type="OrthoDB" id="1026967at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="PTHR11113:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="7">
        <location>
            <begin position="55"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="398" mass="41402" checksum="6A3569C47053B725" modified="2004-10-11" version="1">MGRPIALTGGRVLTPGRELVPATLLIEGTEVAGLAAPGEALPPYAQTIDVSGCLVAPGFIDTHVHGGMGCNFMLGTPEALAVISARLVQGGTTACLATTTSAPARDIAVALDTIARASRAPRPGQVEILGAHLEGPFINPEKAGSQARQHLRPPEPAAVQALWEAAGGALRVVTIAPELPGAGEAIRYLAGMGVQVSLGHSAATYEEAREALGWGVRRATHLYNAMPALQHRRPGAAAALLEDPRVFVELTVDGHHVHPAMVALTYRLVGPERLVLVTDGVDVAGLEDGTYTRWEGTPVRLQGGECRTESGSLAGSTLRLDQAVRNMVRFAGVPVAEALRMASETPAQALGIDRKGRLAPGKDADVVVLSEDLEAILTIARGHVVYDGRPGGRAETIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY9</accession>
    <name>Q67PY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bacterial luciferase-like monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To bacterial alkanal monooxygenase alpha and beta chains.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1269"/>
    <dbReference type="EnsemblBacteria" id="BAD40254">
        <property type="protein sequence ID" value="BAD40254"/>
        <property type="gene ID" value="STH1269"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1269"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_9_0_9"/>
    <dbReference type="OMA" id="AEHHFVA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019949">
        <property type="entry name" value="Luciferase_OxRdatse_group-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03558">
        <property type="entry name" value="oxido_grp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="337" mass="37081" checksum="03F05994B114D5B7" modified="2004-10-11" version="1">MQGMKLSILDLSPIPSGSTASQALQNTLELAQLADRLGYERYWLAEHHNTAAIASVAPEIMIGQVARVTRRIRVGAGGIMLPNHAPLKVAEAFLTLEALFPGRIDLGIGRAPGTDPVTARALRRTASLGAEDFPEQLADLFHFARGDFPEGHPFRSVQATPPGVPLPPVWLLGSSDYSARLAARLGLAFSFAAHFSPFDPVLPMRLYRAQFRPSEHRAEPHAILAVAAICADTRAEAERLARALDLFWLQIRRGERRPLPSPEEAEAYPWTAEELAAVQEHRRLLFLGEPDEVAAGIRSLALATQADEVMVVSHIHDHRQRLRSYELLAEAFALDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q34</accession>
    <name>Q67Q34_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195355.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1224"/>
    <dbReference type="EnsemblBacteria" id="BAD40209">
        <property type="protein sequence ID" value="BAD40209"/>
        <property type="gene ID" value="STH1224"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1224"/>
    <dbReference type="eggNOG" id="COG1873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161336_0_1_9"/>
    <dbReference type="OMA" id="DYVIPWE"/>
    <dbReference type="OrthoDB" id="1995157at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014238">
        <property type="entry name" value="Spore_YlmC/YmxH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40061">
        <property type="entry name" value="PTHR40061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02888">
        <property type="entry name" value="spore_YlmC_YmxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="81" mass="8954" checksum="7A9904E039B5F4F7" modified="2004-10-11" version="1">MAKASDLRKDVIDIRTGRRLGELIDVEIDEKSGRITAIIVPGEGRWFGLWGGGPDIVIPWTKIVCIGPDCILVEIERPMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA5</accession>
    <name>Q67QA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195284.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1153"/>
    <dbReference type="EnsemblBacteria" id="BAD40138">
        <property type="protein sequence ID" value="BAD40138"/>
        <property type="gene ID" value="STH1153"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1153"/>
    <dbReference type="HOGENOM" id="CLU_1004466_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="30508" checksum="FA85CD5459067E1D" modified="2004-10-11" version="1">MSETKWSDAQVEQYLRSLTYADALPDAPALSPELAGRALAAPQRRRAGARWKVGVAAAAVAGLLLVGSNSSMVADAVERILGVGIRNMTQQEYAQRVSGDWPADMPMPEYYSPEETAQLATFPLRSPAWVPEGFTLLNGPAGAFEWLHTEDGQWELLEDPEHFYVTEAYQSADGRRIHIRQSLFGEAQWISWPPGTEQLEVAGHPAFLREDVEPVHVEPPEGVEPTDDWTPDSLNLLYLWVEEPDGRITEITLDGDVDPEVLIQVAESLFAGDGSAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB5</accession>
    <name>SAT_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-sulfurylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfate adenylate transferase</fullName>
            <shortName evidence="1">SAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sat</name>
        <name type="ordered locus">STH1143</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H(+) + sulfate = adenosine 5'-phosphosulfate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18133"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfate adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195274.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QB5"/>
    <dbReference type="SMR" id="Q67QB5"/>
    <dbReference type="STRING" id="292459.STH1143"/>
    <dbReference type="EnsemblBacteria" id="BAD40128">
        <property type="protein sequence ID" value="BAD40128"/>
        <property type="gene ID" value="STH1143"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1143"/>
    <dbReference type="eggNOG" id="COG2046">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022950_1_1_9"/>
    <dbReference type="OMA" id="CPHGQHH"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="UniPathway" id="UPA00140">
        <property type="reaction ID" value="UER00204"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00517">
        <property type="entry name" value="ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00066">
        <property type="entry name" value="Sulf_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025980">
        <property type="entry name" value="ATP-Sase_PUA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020792">
        <property type="entry name" value="SO4_adenylyltransferase_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024951">
        <property type="entry name" value="Sulfurylase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002650">
        <property type="entry name" value="Sulphate_adenylyltransferase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01747">
        <property type="entry name" value="ATP-sulfurylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14306">
        <property type="entry name" value="PUA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00339">
        <property type="entry name" value="sopT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000340632" description="Sulfate adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00066"/>
        </source>
    </evidence>
    <sequence length="393" mass="43812" checksum="B793C344C5E53D89" modified="2004-10-11" version="1">MATQIAPHGGRLVDRWLRGPAREEALERARRLPRVRLDAREAADLEMIGDGALSPLTGFMGQADYRSVVAEMRLASGLLWALPVTLAVSRAEAESIREGEEIALEDPGGRLMAVMRVAERFAYDRGAEAARCYGTTDPAHPGVRRLLRQGEVYLGGEVWLLDRPPAPFAEYRLTPAETRAEFARRGWRTVVGFQTRNPVHRAHEYIQKCALEICDGLLLHPLVGETKDDDLPAAVRMRAYEAILEGYFPRERILLAVFPAAMRYAGPREAVWHALCRKNYGCTHFIVGRDHAGVGSFYGPYDAQRIFDHLDPAELGITPLFFDHTFWCRTCGAMASPKTCPHGPEARVALSGTRVREMLYRGEAPPPEFTRPEVARVLMEGLQAAARPQSVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD8</accession>
    <name>Q67QD8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative mannose-1-phosphate guanyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195252.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1120"/>
    <dbReference type="EnsemblBacteria" id="BAD40105">
        <property type="protein sequence ID" value="BAD40105"/>
        <property type="gene ID" value="STH1120"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1120"/>
    <dbReference type="eggNOG" id="COG1208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_2_9"/>
    <dbReference type="OMA" id="PFLTHQL"/>
    <dbReference type="OrthoDB" id="1557977at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="36621" checksum="A63897D3C31125BA" modified="2004-10-11" version="1">MAVRAILLAGGLGTRLHPLTVELPKPMVPVLGKPWLSRLIDQLAAFGVTDITLSLRHGGQVVTDYFRESPPGVRLRFAVEPQPLGTGGAIRFAAGPDPTDTLLILNADIVQTFDLNALLEFHRQHRAQVTIGLVEVADPSAYGAVELDKNSRVTRFVEKPRPGETDSRMVNAGVYVFDPGVLSWIPPERPVSVERETFPALLRDGVRVYGCLCTGYWKDIGTGDRYLQLHRDILEGRCPIPVSGPVRGPAHWQADDADVSPDARIMPPVYIGSGAVIEAGAQVGPRAVIGAGCLVAKGARISDSVLWDGARVGAGATVRHSVIGFATGIGGGTVENVLLAGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF0</accession>
    <name>Q67QF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195240.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1108"/>
    <dbReference type="EnsemblBacteria" id="BAD40093">
        <property type="protein sequence ID" value="BAD40093"/>
        <property type="gene ID" value="STH1108"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1108"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_9"/>
    <dbReference type="OMA" id="AAWKHGT"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="98"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="36644" checksum="70D360D61E18C46A" modified="2004-10-11" version="1">MKAVRILERVLTALPMSILMLLLVFAFVRLLPGDPVDIMMGQAGNVSQAEIDALRRELHLDRPLAEQLSIFVRGLLRGDLGYSFREQRPVAAIIGEAFPATVELALAGLTFALLVGLPIGVVSAVKRNSWFDRLAMGFSFLGISMPAFWLGIMAMMLFSVHLGWTPVQGRLSAGLVPPDLTGFYVLDALLARDWALLKDALRHLVLPAVTLGAELTAIIARVTRSSMLEALHMDYVTAARSRGVPEWQVVLRHALRNALIPTVTVTGLQLGVLLGGNMIVETVFGWPGLGRLVVGAIFARDYALVQGAVMLYALTFLLANLLVDILYTQLNPKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF8</accession>
    <name>Q67QF8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195232.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1100"/>
    <dbReference type="EnsemblBacteria" id="BAD40085">
        <property type="protein sequence ID" value="BAD40085"/>
        <property type="gene ID" value="STH1100"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1100"/>
    <dbReference type="eggNOG" id="COG3307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504236_0_0_9"/>
    <dbReference type="OrthoDB" id="754057at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="330"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="516"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="540" mass="57725" checksum="182B106FA8EC46D3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI0</accession>
    <name>Q67QI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195210.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1078"/>
    <dbReference type="EnsemblBacteria" id="BAD40063">
        <property type="protein sequence ID" value="BAD40063"/>
        <property type="gene ID" value="STH1078"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1078"/>
    <dbReference type="eggNOG" id="COG1683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076318_1_1_9"/>
    <dbReference type="OMA" id="CPECAGG"/>
    <dbReference type="OrthoDB" id="1757306at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007553">
        <property type="entry name" value="2-thiour_desulf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04463">
        <property type="entry name" value="2-thiour_desulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="156" mass="16306" checksum="14FF2D85C5218C5F" modified="2004-10-11" version="1">MILVSACLVGCRCRYDQGAKPHDPVIVLIREGKAVPVCPEQMGGLPTPRLPAEIIGGDGDDVLDGRARVVNAAGEDVTEQFLAGAREALRMAEAAGATVAILKERSPSCGSAAIYDGTFRGTVRPGQGVTAALLRRHGIEVLSEESWPEGTGEVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV6</accession>
    <name>Q67QV6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Permease IIC component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH952</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH952"/>
    <dbReference type="EnsemblBacteria" id="BAD39937">
        <property type="protein sequence ID" value="BAD39937"/>
        <property type="gene ID" value="STH952"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH952"/>
    <dbReference type="eggNOG" id="COG1455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029688_1_1_9"/>
    <dbReference type="OMA" id="WYPFFKA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004501">
        <property type="entry name" value="PTS_EIIC_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004796">
        <property type="entry name" value="PTS_IIC_cello"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006351">
        <property type="entry name" value="PTS_EIIC-Cellobiose"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51105">
        <property type="entry name" value="PTS_EIIC_TYPE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-3" evidence="10">
        <location>
            <begin position="11"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="413" mass="45551" checksum="9BBF3BF8A8D51F46" modified="2004-10-11" version="1">MSFMQRFMDWMQNSVGPATEKVANNAWISGLQKAILKALPMVLVGSLITIYNVVRNFAPSLPDLRPISTYTFGLISLFMVFCVPYYILELKNVHKTKFVAGFTGIALFFIMLNPTVTDQGYVFNFSGFGAGGMFVAIVAGLFTAAVMLLFRRLTFFKEDSVMPDFVKEWFDSMLPIFVVVFVGWLVVIRWGFDIYSAIVALFTPLMSIAQSLPGMILLYLIPTVFYSMGISGWVFQPILNPVQLFAINANAEAAAAGAVAQYPFTAETTYAFLSLGGRGATLSLVLLLLFAHSKRLKALGRASSVPAVLNINEPVVFGTVAWNPILMIPMWLNGLILPLITYFALKTGLVPIPTEVFTMWYVPIGISSWLVTKSVSGLILTAVNFLVSLAIWFPFFKLYDSQEAAKEAQAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R35</accession>
    <name>Q67R35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfate ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH873"/>
    <dbReference type="EnsemblBacteria" id="BAD39858">
        <property type="protein sequence ID" value="BAD39858"/>
        <property type="gene ID" value="STH873"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH873"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_10_0_9"/>
    <dbReference type="OMA" id="VQEPWGA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269945" evidence="1">
        <location>
            <begin position="21"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="35436" checksum="0CA71A64A097D42E" modified="2004-10-11" version="1">MRRCLAILVVVLLLAGCSAAREGSEQSGAPPGEVRIGYMPNLTHAQAVLGVAEGTFARHLGVPVRPRLFTSGSAALQALFAGEVDLLYIGPAPALQGYLRSDGDALRVIAGAASGGSVLVLRPDVDRDDLRGTRLATPGIGNSQDAALRYLLMQEGWRTRERGGDVTVSPMAPAEILTLFSRGELDGAWVAEPWGSRLIAEAGGVLAIDERELWPDGTVPTTLVAVRPGFLSEQPEAVRRFLEAHVSLTREIQADPDGSRERVRQALAELQGRPLSDLVMADAWARVDFTFDPMADAVAELADRAFRAGLLGASRPDLTSLYDLTLLQEVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R53</accession>
    <name>Q67R53_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH855</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194987.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH855"/>
    <dbReference type="EnsemblBacteria" id="BAD39840">
        <property type="protein sequence ID" value="BAD39840"/>
        <property type="gene ID" value="STH855"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH855"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055576_0_0_9"/>
    <dbReference type="OMA" id="NINDSIW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="24"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="300"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="403" mass="42144" checksum="BF9787A24828A371" modified="2004-10-11" version="1">MRFSERPGILRLAARNLLRNPGRTLVVLLGVMVVAGTAFAGGLIGRGVSFAVSRGLERLGADLMVVPEGAAEKTHTALVMGLPTAFYMEGAGRLEAIRAVEGVAAASPQIFVETLASSACCTGHLMLVGYDPETDFTVKPWLRQNLKRELAPDEVLVGNHILGVTGDPLLFYGSTFRLAARLDPTGMGMDETVFLPAPAVWEIAERSRELATEPLEIPQGHVSAILVKLEDGADAAQVAAEIERRLDGVTVITAGEIARSVADDLSVLMASLLPVLISLVLVAVLLFVILFFAIARERTREIGLLRAMGATAGQATGALVLEAGLLSALGGLAGVLAGSAVYGLFKEAIMVSYTLPFLYPPGAEQTLLAAAVVAVAAAGGVLAAAVPARRLARLEPHEAIHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R71</accession>
    <name>Q67R71_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pfkA</name>
        <name evidence="16" type="ordered locus">STH837</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 14">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade 'B1' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH837"/>
    <dbReference type="EnsemblBacteria" id="BAD39822">
        <property type="protein sequence ID" value="BAD39822"/>
        <property type="gene ID" value="STH837"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH837"/>
    <dbReference type="eggNOG" id="COG0205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020655_0_1_9"/>
    <dbReference type="OMA" id="KFAVICV"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00339">
        <property type="entry name" value="Phosphofructokinase_I_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012003">
        <property type="entry name" value="ATP_PFK_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012828">
        <property type="entry name" value="PFKA_ATP_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000532">
        <property type="entry name" value="ATP_PFK_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="SSF53784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02482">
        <property type="entry name" value="PFKA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 14 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PFK" evidence="15">
        <location>
            <begin position="6"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator; shared with dimeric partner" evidence="14">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="14">
        <location>
            <begin position="128"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="14">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator" evidence="14">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP binding; allosteric activator" evidence="14">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="14">
        <location>
            <begin position="252"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD39822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="34145" checksum="E3198AF8A92E76DE" modified="2004-10-11" version="1">MPKIRKIGVLTSGGDAPGMNAAVRAAVRRGISLGAEVVGVRRGYAGLIAGEFVDLGVSSVGDIIHRGGTFLQTARSDHFLTPEGQAQALAKAREAGIEGLVVIGGDGSFRGALRLTELGLPAVGVPATIDNDIYGTDLTIGFDTAVNTCLDAIRKIRDTATSHERTFVLEVMGRHSGWIALAAGLAGGAESILLPERPMPMEEVVTRLKRGVERGKRHTLIIVAEGVGSGFEVGRQIREMTGYDTRVMVLGHIQRGGSPTALDAMLASRMGAKAAEVLLEEGRGGLFIGVENEQMVTRPLEQVLGRRKEIPDELVDLAEILAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R92</accession>
    <name>Q67R92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation initiation factor eIF-2B alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194949.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH816"/>
    <dbReference type="EnsemblBacteria" id="BAD39801">
        <property type="protein sequence ID" value="BAD39801"/>
        <property type="gene ID" value="STH816"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH816"/>
    <dbReference type="eggNOG" id="COG0182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_2_0_9"/>
    <dbReference type="OMA" id="DMVTQSA"/>
    <dbReference type="OrthoDB" id="1290468at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="38703" checksum="A0B169767FF249AD" modified="2004-10-11" version="1">MNGPKLPLLTNRADTLKYEDGVVRILDRRRLPEETVFVDCRDYREVARAIVDMVIQGAPPLAYAAGYGLALACRQSRDLPAPERRRALEQAHRELRATRPTGADIFPLLDRALALALAALEEGRDPEAAVREFVDGQIRAGDRAARLCGSHAAGLLEDGDRILTHCFAGAALNYMLYFALKAGKRIELIATETRPYLQGARLTAAQAREIGVPVTLVTDNMPGYLFYRGMITKYVTAADSISLDGHVSNKIGTFQYAVLAHHFGVPFMVLGYDGPDRERATAADIPIEERDPEEVFYCRGVRTAAEGIRGYYPAFDITPPQYVSVIATDRGLFSPWNVRDYFRAPEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL4</accession>
    <name>Q67RL4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH694</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194827.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH694"/>
    <dbReference type="EnsemblBacteria" id="BAD39679">
        <property type="protein sequence ID" value="BAD39679"/>
        <property type="gene ID" value="STH694"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH694"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049568_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012046">
        <property type="entry name" value="LytTR_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007492">
        <property type="entry name" value="LytTR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04397">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036612">
        <property type="entry name" value="ABC_ATP_LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00850">
        <property type="entry name" value="LytTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50930">
        <property type="entry name" value="HTH_LYTTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="HTH LytTR-type" evidence="5">
        <location>
            <begin position="224"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="35198" checksum="00E07C80FBBAD8E3" modified="2004-10-11" version="1">MIRVDGLTIAGKLTDIRLTVQPGELVAVVGSRGSGKSTLVRALVGLVPPDGGTVTVGGHPAGSDAGRALVGAASEAWGLMERLTVWENLNTFARLRGAPRDRAAALLNRLDLGGRRDVRVDRLTPGEAARLRLACALVHDPSVLFLDEPAGDIDSESASLIAFTVAQEAEAGKTVLVMTFGDPRILGVASRICYLEGGRLVEPEAAAAGEPAPPSAVRRPVPHVAARKGDRVLLFRPEEIRFAYARDKAVYIETPEGTCGVSFTLAELEERLADSGFFRSHKAYLVNLAYVREIAAWTRDSYSLILKDGEEVPLSKHRAHELRVRLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP2</accession>
    <name>Q67RP2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH666"/>
    <dbReference type="EnsemblBacteria" id="BAD39651">
        <property type="protein sequence ID" value="BAD39651"/>
        <property type="gene ID" value="STH666"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH666"/>
    <dbReference type="HOGENOM" id="CLU_1439701_0_0_9"/>
    <dbReference type="OrthoDB" id="1593228at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09670">
        <property type="entry name" value="Cse2_I-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013382">
        <property type="entry name" value="CRISPR-assoc_prot_Cse2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038287">
        <property type="entry name" value="Cse2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09485">
        <property type="entry name" value="CRISPR_Cse2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02548">
        <property type="entry name" value="casB_cse2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="191" mass="22009" checksum="FABDA933DD82397C" modified="2004-10-11" version="1">MKGPSSRQVEFAGWLSGLDDRGTLAALRRGLMLEEEQLFELFGYVPPRFLTGLRPGEERLYLMVAALYAYHPVSFGEEELAERRRNLGESLRRLAEEKARQRGGLDEGEELLPESLKRRMEALLSAPRADLFGHLRQVISLLKSEEIPVDWAQLLSDLQRWEAPDRRVQWAWSRSFYVGYQTEGGDETDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP4</accession>
    <name>Q67RP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH664</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH664"/>
    <dbReference type="EnsemblBacteria" id="BAD39649">
        <property type="protein sequence ID" value="BAD39649"/>
        <property type="gene ID" value="STH664"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH664"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013924_1_0_9"/>
    <dbReference type="OMA" id="MATGNAM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041372">
        <property type="entry name" value="Cas3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF5">
        <property type="entry name" value="PTHR47959:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18395">
        <property type="entry name" value="Cas3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18019">
        <property type="entry name" value="HD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD Cas3-type" evidence="9">
        <location>
            <begin position="30"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="936" mass="105027" checksum="96AA97C8AF3EF76E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ8</accession>
    <name>Q67RQ8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH650</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194783.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH650"/>
    <dbReference type="EnsemblBacteria" id="BAD39635">
        <property type="protein sequence ID" value="BAD39635"/>
        <property type="gene ID" value="STH650"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH650"/>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545034_0_0_9"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="406"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="187"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="500" mass="54125" checksum="4DDA36146C7E50AB" modified="2004-10-11" version="1">MHRLAPQHRLFALLLAIGLTPIVLLPALGLAPRWLFWAAPPLALLLYLLSRQFTRPVSEVMALARRLREGDLRARAVLPPGTDWFMLARTMTDLAEQLEAVTRNLEEQVAVRTAALARKADQLRAVGQVGQQVAAVLDPDVLLHFVTRVMRGTFGYDVAAVLLQHGDYLVLAACAARGREEVPLGRAFPLTGPTPSPLARALAAGEPGGERAEGEHPESGPPTPLLPDTAPRAELAIPIRVADRTLGLMVVQSFDPRPFDEEDRFTVQTIAGQVAVALENARLLEAERRLRRLAVTEERNRMAREIHDTLAQGFMGILMQLRAMEAAADPETARFHREVAAALAQESLQEARRSVWKLRPHPLEDRGLAEALAAEVAALEGQFGLIGRFHMDGDAAALEPPVEVALLRIAQEAIHNAVKYARATRLDVGLTIRDGMAVLTVADDGVGFDPASPPKPAAGGGYGLGMMADRARQVGGGLEVDARPGGGCRIVARVPLRKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ9</accession>
    <name>Q67RQ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH649</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194782.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH649"/>
    <dbReference type="EnsemblBacteria" id="BAD39634">
        <property type="protein sequence ID" value="BAD39634"/>
        <property type="gene ID" value="STH649"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH649"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_9"/>
    <dbReference type="OMA" id="HVHRILQ"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="145"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="23023" checksum="380918607F1B944C" modified="2004-10-11" version="1">MAVRVVVVDDHRVVRAGLVALLRQAPGLEVVGEAGNGQEALDVVEATKPDVVLMDMQMPVMNGVEATRQIRARFPDVEVLVLTTYDDDELIWGGIQAGAKGYLLKDAPPQQLLEGIETVARGQSLLSPEIAAKLVQVISQGGPARQEGAEGLTERETEILRLIASGAANKEIAAALFISENTVKTHISNLFQKLGARDRTEAVTKALARGWLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR9</accession>
    <name>Q67RR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194772.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH639"/>
    <dbReference type="EnsemblBacteria" id="BAD39624">
        <property type="protein sequence ID" value="BAD39624"/>
        <property type="gene ID" value="STH639"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH639"/>
    <dbReference type="HOGENOM" id="CLU_2541359_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="10301" checksum="662AA7F58260CEA9" modified="2004-10-11" version="1">MGTRQRRPGTGTRRLRRGRPWMRGRGRRRTGWLAALVGRLRRRWRSFWHWEVLHALRERYPQLSRTRTLSPGELRRFPQRTWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX2</accession>
    <name>Q67RX2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Pyruvate, phosphate dikinase</fullName>
            <ecNumber evidence="4 7">2.7.9.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH586</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + phosphate + pyruvate = AMP + diphosphate + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10756"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.1" evidence="4 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH586"/>
    <dbReference type="EnsemblBacteria" id="BAD39571">
        <property type="protein sequence ID" value="BAD39571"/>
        <property type="gene ID" value="STH586"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH586"/>
    <dbReference type="eggNOG" id="COG0574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015345_0_2_9"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PTHR22931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000853">
        <property type="entry name" value="PPDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01828">
        <property type="entry name" value="pyru_phos_dikin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="12">
        <location>
            <begin position="19"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="PPDK_N" evidence="12">
        <location>
            <begin position="302"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="11">
        <location>
            <begin position="420"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="13">
        <location>
            <begin position="516"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="8">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="840"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="558"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="775"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="776"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000853-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD39571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="892" mass="98743" checksum="01F2882BD6032280" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S09</accession>
    <name>Q67S09_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194683.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH549"/>
    <dbReference type="EnsemblBacteria" id="BAD39534">
        <property type="protein sequence ID" value="BAD39534"/>
        <property type="gene ID" value="STH549"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH549"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_14_3_9"/>
    <dbReference type="OMA" id="WYEINSD"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="108" mass="12429" checksum="269F4A59EAB7DC4B" modified="2004-10-11" version="1">MQRLTAETFDEAVREHPHLIVNFTADWCPDCRRIKDAYAGFPERFPEIAFAVVDTEESPDLAERFDVRGIPSLLVFRDGELIDRLYSRDAKTVQQVEAFVTRQLPVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE0</accession>
    <name>Q67SE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH418"/>
    <dbReference type="EnsemblBacteria" id="BAD39403">
        <property type="protein sequence ID" value="BAD39403"/>
        <property type="gene ID" value="STH418"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH418"/>
    <dbReference type="HOGENOM" id="CLU_1748730_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005370">
        <property type="entry name" value="UPF0180"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03698">
        <property type="entry name" value="UPF0180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="16239" checksum="710F46279B997BE4" modified="2004-10-11" version="1">MIQRPGLRATTRAGTGAEPTAAAAAERLRRRPDARQILLPRRGHGSRRPAQTKFWPPDPYDQTCAEVLSMRIAVQDGLENVKQALRAEGYQVTRLTPGSMEGVGAAVVTGLSNNFMGIHDTDGNLFPVIDATGKTVEEIVRQVKERGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF8</accession>
    <name>Q67SF8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH400"/>
    <dbReference type="EnsemblBacteria" id="BAD39385">
        <property type="protein sequence ID" value="BAD39385"/>
        <property type="gene ID" value="STH400"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH400"/>
    <dbReference type="HOGENOM" id="CLU_1348355_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="1">
        <location>
            <begin position="42"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="21679" checksum="8E1C7583D20A0F10" modified="2004-10-11" version="1">MTGMNSRTRYLLAALCAAAAGLLTFLYLGGIGVAAGSGTEIVWVARREILPGTQLKEEMLQRVEVDGPTRQLLAREALPRTASDTPDGWYATRAIRPGEPLIPAGNVSPVPPSADVTPPEALRVVSLRTEWVGPPELQPTEEVDLYVVTGDGEALRILTGARVVQAESDRVSVLVPEEQVPLVIAAADGVTVKVVRRLEGLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH5</accession>
    <name>Q67SH5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tungstate ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194517.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH383"/>
    <dbReference type="EnsemblBacteria" id="BAD39368">
        <property type="protein sequence ID" value="BAD39368"/>
        <property type="gene ID" value="STH383"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH383"/>
    <dbReference type="eggNOG" id="COG4662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_2_9"/>
    <dbReference type="OMA" id="EVGVAMM"/>
    <dbReference type="OrthoDB" id="1110665at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="26"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="24775" checksum="30CAE3B26236D777" modified="2004-10-11" version="1">MEMIGQGILAALRMLVSGDAELVRVTLLTIRVAGTATLLSILVGIPLGCFLALARFPGREFMISIVNAGMGTPPVVAGLVVSLLLWRSGPFGYLRLMYTPTAMIIAQFLIALPLVTGYALAGIGQLNPKLHDQILALGASRWQLYWLLIREARLPLLAAVIGGFGGVISEVGASTMVGGNILGRTRVLTTATVMEVSKGNFDIAIALSIILMLLIWLVTYALTLLQQRGRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK3</accession>
    <name>Q67SK3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH355"/>
    <dbReference type="EnsemblBacteria" id="BAD39340">
        <property type="protein sequence ID" value="BAD39340"/>
        <property type="gene ID" value="STH355"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH355"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_13_3_9"/>
    <dbReference type="OMA" id="ICHHGAR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="22"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="12153" checksum="95F8CBF67A04775F" modified="2004-10-11" version="1">MFGFGPRIESITTAELQARLERGERPKIIDVREPWEYAEGHVPGAVLKPLGQIRTWARELDRNEEILLICRTASRSAVAYQFLKRLGFTNVKNVAGGMVTWRGEVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS8</accession>
    <name>Q67SS8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5808 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39265">
        <property type="protein sequence ID" value="BAD39265"/>
        <property type="gene ID" value="STH280"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH280"/>
    <dbReference type="eggNOG" id="COG4194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2620757_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043831">
        <property type="entry name" value="DUF5808"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19124">
        <property type="entry name" value="DUF5808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5808" evidence="2">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8738" checksum="CA5D113411FEC0D4" modified="2004-10-11" version="1">MSGGSSGWSAPRHWKLGILYYHYPEDRRMVVPRRRGMGMTLNSAHKHTWFFVGALFLPLVTVLLLILVSTAAGEISVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST2</accession>
    <name>Q67ST2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH276"/>
    <dbReference type="EnsemblBacteria" id="BAD39261">
        <property type="protein sequence ID" value="BAD39261"/>
        <property type="gene ID" value="STH276"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH276"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_8_2_9"/>
    <dbReference type="OMA" id="WIANWEN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="29106" checksum="733BCCC0F28E67C9" modified="2004-10-11" version="1">MAQFRNRPLDGEYPYVWLDAKAVKTRENDRVVNMAAVVAVGVRTTGDREVLGFDLGPAETYEFWVTFLRSLVRRGLGGVRLVISDAHEGLRQAIAEVLQGAAWQRCRVHFMRNLLGYVPKPAQSMVAALVRTVFAQPDIEAAREQLDRVVASLGRRYPKVAALLSEAAEDVLAYMAFPREHWQKIHSTNPLERLMREIGRRVDVVGIFPNAAAALRLIGAVLQEQEDEWRVQRRYLSMQSMAKLALTPSEPEAALVLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX2</accession>
    <name>Q67SX2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH236</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194371.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH236"/>
    <dbReference type="EnsemblBacteria" id="BAD39221">
        <property type="protein sequence ID" value="BAD39221"/>
        <property type="gene ID" value="STH236"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH236"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_9"/>
    <dbReference type="OMA" id="GNIAIEM"/>
    <dbReference type="OrthoDB" id="1325025at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="59"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="27982" checksum="C72FEA0705B55516" modified="2004-10-11" version="1">MDVRDRLARAALGAAATVASAGVILIALAVTAEGWRALPHLFGPVWSPEDGRFGVLPLLAGSVLVTLGSLALAVPVGVGVGLFLTEVATPRLAGAVRPVLQGMAAVPSVVFGFLGLNLIVPWVSRAFGGLGLSLLAGWLVLALMVLPTVAAVSEDALRAVDPALREGAYALGATPGQVAWRVLLPAAKGGITTAVILALGRALGETMAVLMVLGNAAAVPRSPLDPARTLTGNIALEMAYATGRHREALFASGALLLLINLAVTQAARRAAGGRAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T15</accession>
    <name>Q67T15_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194328.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH193"/>
    <dbReference type="EnsemblBacteria" id="BAD39178">
        <property type="protein sequence ID" value="BAD39178"/>
        <property type="gene ID" value="STH193"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH193"/>
    <dbReference type="eggNOG" id="COG4856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039811_1_0_9"/>
    <dbReference type="OrthoDB" id="992089at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012505">
        <property type="entry name" value="YbbR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07949">
        <property type="entry name" value="YbbR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="424" mass="45852" checksum="5D715548E7AE78EA" modified="2004-10-11" version="1">MIRRLLALLRHDFWLKLLSLAVAVLLWAMVVQDYNKVTSVTFDVPLEVISHPEYEIYEGRRDLETEVEVRVTGPNLLVSSLKADDLRAWVDYRTVQQAGRAQEVRVQVSGPPRYEGQVEYRANPATVTVTLVENRTLTVPVAVTPESGIVAVGDREFRYTAVPVNGTIFVSGRADYLGLVRSAVVALQGADLEPPLQDGRLTETRTRLQKAVRLVDAVMQPVEKLAEHYAEVEITWEELPPGKRVAVSPQTVGELPPGFELAGVSVSPAEVTIRSATLDGSLPQVSAVQTEPVDLSGQTQSFTTVARLVAPAGTSLAQTSVEVAVSIREVSAEKLFGAVPIAVQNAPEGLEVTLSQGTVEVRLTGRYTLVHPLDASAVIAYVDVAGLGPGTHRLPVKVIYPPEIVETAIDPAVIEVTISPAEIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T19</accession>
    <name>WHIA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cell division protein WhiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">whiA</name>
        <name type="ordered locus">STH189</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell division and chromosome segregation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WhiA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T19"/>
    <dbReference type="SMR" id="Q67T19"/>
    <dbReference type="STRING" id="292459.STH189"/>
    <dbReference type="DNASU" id="2979884"/>
    <dbReference type="EnsemblBacteria" id="BAD39174">
        <property type="protein sequence ID" value="BAD39174"/>
        <property type="gene ID" value="STH189"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH189"/>
    <dbReference type="eggNOG" id="COG1481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053282_0_0_9"/>
    <dbReference type="OMA" id="CDAEAAW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043937">
        <property type="term" value="P:regulation of sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01420">
        <property type="entry name" value="HTH_type_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018478">
        <property type="entry name" value="Sporu_reg_WhiA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003802">
        <property type="entry name" value="Sporulation_regulator_WhiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023054">
        <property type="entry name" value="Sporulation_regulator_WhiA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039518">
        <property type="entry name" value="WhiA_LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37307">
        <property type="entry name" value="PTHR37307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02650">
        <property type="entry name" value="HTH_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14527">
        <property type="entry name" value="LAGLIDADG_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10298">
        <property type="entry name" value="WhiA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00647">
        <property type="entry name" value="DNA_bind_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000376602" description="Probable cell division protein WhiA">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="282"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01420"/>
        </source>
    </evidence>
    <sequence length="314" mass="34851" checksum="AB0D950B51275ECB" modified="2004-10-11" version="1">MSEELSFSAMVKAELALLTPELPCCRRMELAGLVRALGRLELSGRGRFALTLSTDSAPVSRKVIRLLKSVSPVRYEVMVMRRRKLRKNLVYRVHIPHQPGVAELLQLAGFIDEAGRPADWVEPPELANDHCRRAFLRGTFLGSGWVAGPEKQHHLEITTTATEAADALGQMLFRGGIAARMVARRESLVLYIKEADQIIRFLGLVGAHQALLRYEEVRVIKEMKNQVNRQVNAEVANMTKQADAAARQVEAIKRLEAAGALERVSPPLRELAGLRLAYPDASLKELGELCRPPVSKSGAAHRMRQLMALAESLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T90</accession>
    <name>METK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine synthase</fullName>
            <shortName evidence="1">AdoMet synthase</shortName>
            <ecNumber evidence="1">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">MAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metK</name>
        <name type="ordered locus">STH118</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194253.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T90"/>
    <dbReference type="SMR" id="Q67T90"/>
    <dbReference type="STRING" id="292459.STH118"/>
    <dbReference type="EnsemblBacteria" id="BAD39103">
        <property type="protein sequence ID" value="BAD39103"/>
        <property type="gene ID" value="STH118"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH118"/>
    <dbReference type="eggNOG" id="COG0192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041802_1_1_9"/>
    <dbReference type="OMA" id="MPYLRPD"/>
    <dbReference type="OrthoDB" id="1024388at2"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000174608" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible loop" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="258"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00086"/>
        </source>
    </evidence>
    <sequence length="398" mass="43760" checksum="AB30DC16785C51AC" modified="2004-10-11" version="1">MTEQKGRYLFTSESVTEGHPDKMADQISDAVLDAILALDPRARVACETLLTTGLVVVTGEITTHAYVDIPNLVRDVVRDIGYTRAKYGFDGSTCGVMTAIDPQSPDIAQGVDVAIEVRGEVNEKELEIGAGDQGMMFGFACDETPELMPLPISLAHRLARRLAQVRKNGTLPYLRPDGKTQVTIEYEDGRPKRIDTIVISAQHDPDTTQEQIRKDVIDYVILDVVDRNLIDDRTRYFINPTGRFVVGGPQGDTGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARWVAKNIVAAGLARKCEVQVAYAIGVAHPVSILVTTFGTGKLPDERLAQLVRETFDLRPGAIIRDLDLRRPIYRQVAAYGHFGRPDLDLPWERTDKVDLLREKAGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T95</accession>
    <name>RS4B_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4 B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsD2</name>
        <name type="synonym">rpsD</name>
        <name type="ordered locus">STH113</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194248.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T95"/>
    <dbReference type="SMR" id="Q67T95"/>
    <dbReference type="STRING" id="292459.STH113"/>
    <dbReference type="EnsemblBacteria" id="BAD39098">
        <property type="protein sequence ID" value="BAD39098"/>
        <property type="gene ID" value="STH113"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH113"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_1_9"/>
    <dbReference type="OMA" id="HIPFEFN"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000228932" description="30S ribosomal protein S4 B">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="93"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <sequence length="201" mass="22874" checksum="EBE35A1D0466AE80" modified="2004-10-11" version="1">MARYIGPKHKLCRRVGRPLCGSAKCPALKRPYRPGQHGPGRPQKLSEYGAQLLEKQKLRFIYGVMERQFRRYFEQAQRSRGVTGEVLLQLLEQRLDTVVYRLGFARTMAAARQLVGHGHVTLNGRRVDIASCQVKPGDVVGLTQKARSLAVVRESLDLRRPVPPYLSLDETTMTSRLQRLPLREEIPVDVDESLVVELYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB0</accession>
    <name>Q67TB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH98</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH98"/>
    <dbReference type="EnsemblBacteria" id="BAD39083">
        <property type="protein sequence ID" value="BAD39083"/>
        <property type="gene ID" value="STH98"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH98"/>
    <dbReference type="eggNOG" id="COG3552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_663785_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008912">
        <property type="entry name" value="Uncharacterised_CoxE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05762">
        <property type="entry name" value="VWA_CoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="414" mass="47019" checksum="6B9486052CB57598" modified="2004-10-11" version="1">MCSIGRLIRRLMMRAGRLFRLRRPRLVAAAQVTRLFLAGRDPNNLYVAFAEAADHRPEAPASLAEVQAGDRMTSLLWAGPAAADPVEPVAARASLWRGLAPSPADDALRGAEAANRRFAYVDLLHSPIRKLAPEHIRELEELMDRLARKWLAHRDTFRERPRRARLDVGRTLRHNLPRYGGAVLEFRWPVKVVPVPQPQKPSRILVIGDVSHSMARYVSVVLFFFHKLNFRFVVESYVFSENATYATPYLNGPGTFEEKAQRLMQGARSWNAGTRFGTALAEIAQHAAVDDQTYVFIATDGKVAITPDEQEKIRVNMAELRRRARQVIFLTPSAEFSGVRRGPAREWQRRLGTFYYGPNEVPVWLMGSPHWYGTLARYADRLYLIRTVQDLIDMVENLLISSGEEERRNGHGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF8</accession>
    <name>Q67TF8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH50</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH50"/>
    <dbReference type="EnsemblBacteria" id="BAD39035">
        <property type="protein sequence ID" value="BAD39035"/>
        <property type="gene ID" value="STH50"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH50"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_8_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017895">
        <property type="entry name" value="HTH_IS408/IS1162_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50532">
        <property type="entry name" value="HTH_IS408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH IS408-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="8322" checksum="A305251C9E4589DA" modified="2004-10-11" version="1">MANRRLSMRKIREILRLKWEVGLSARQIARSLSISHSTVLDMLRRFECSGLSWPLPNIDDAELEAKLYPPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH7</accession>
    <name>Q67TH7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sigma-54-dependent transcriptional regulator variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH31</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH31"/>
    <dbReference type="EnsemblBacteria" id="BAD39016">
        <property type="protein sequence ID" value="BAD39016"/>
        <property type="gene ID" value="STH31"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH31"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_1_9"/>
    <dbReference type="OMA" id="QICNARP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="2">
        <location>
            <begin position="140"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="438" mass="47894" checksum="CE547DAEE2EB83CC" modified="2004-10-11" version="1">MIDEGIHVVDRNGVTVLYNQQAGQNDGLSPEEVIGRHLLEVFPSLTPATSTLLKVLATGKPIPAHEQTFANYKGQRVTTINSTLPIFHEGELVGAIEVSKDVTRVRELSERVAGLQAALLRPRSRRGAPVGGARYTFDDLVGDHPAMQRLKEQALRAAATDSPVLVYGETGTGKELLVHAIHAASPRRHRPLVAQNCAALPEGLLESLLFGTARGSFTGAEDRPGLFELADGGTLYLDEINSMQPELQAKLLRVLQDGRIRRLGEATERQVDVRIIASTNEEPAAALASGRLRSDLYYRIAVVALEMPPLRRRASDIPLLTDHFLDKHSARLGLPRRPLHPEVEAFFSGTPGRATCASWSTPSRRRCSWPPGRRSGSAISRLPCSGQPRRRSGAAGRMRAGRGPLPQTRGFRPVSCAASWRSWSGRRCGRPWRRRGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ9</accession>
    <name>Q67TJ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH9</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194144.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH9"/>
    <dbReference type="EnsemblBacteria" id="BAD38994">
        <property type="protein sequence ID" value="BAD38994"/>
        <property type="gene ID" value="STH9"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH9"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_9"/>
    <dbReference type="OMA" id="NIAGMQV"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="462"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD38994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="540" mass="57726" checksum="B1624D02E7983C59" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J60</accession>
    <name>Q67J60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197421.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3309"/>
    <dbReference type="EnsemblBacteria" id="BAD42290">
        <property type="protein sequence ID" value="BAD42290"/>
        <property type="gene ID" value="STH3309"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3309"/>
    <dbReference type="eggNOG" id="COG0308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079582_0_0_9"/>
    <dbReference type="OMA" id="MGVYWAG"/>
    <dbReference type="OrthoDB" id="1514411at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269495" evidence="1">
        <location>
            <begin position="33"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="33831" checksum="D6025AEEFB1E9626" modified="2004-10-11" version="1">MKGQRVVWRLLSAVVAGALLLCGLAGWPRARAAVYRYYRHQGRVRTLGGLAGYRTAESGRFVLYYLPEDGDLAALILEQAEAVYERVVAEVGYPPAERVPLILYPDRTALRAAYGWGSEQSAVGVYWRGTVGLLSPRVWIAAGDPAELASEFRRLSPVAHELTHYLLDELTEGNYPRWFTEGLAQYVEELATGYVWPEAAAAPLDPPLYTLSELTRRFDDLPDRPLAYRQSHLLVAHIAEAHGRPGLTGLIALLAEGVPFDRAVETALGRSMASIYADWLDQAEGRSAQGRNVRLSERKGLLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J61</accession>
    <name>Q67J61_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3308</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197420.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3308"/>
    <dbReference type="EnsemblBacteria" id="BAD42289">
        <property type="protein sequence ID" value="BAD42289"/>
        <property type="gene ID" value="STH3308"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3308"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="IDRVWGS"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="133"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="27142" checksum="17E273365EDD22F3" modified="2004-10-11" version="1">MADRILVVDDERPIAEILKFSFEREGFEVEMAFDGEEAVAKARASDFDLVILDIMLPKLDGFTVCREIRRFSTVPILMLTAKEDEVDKVLGLELGADDYVTKPFSPRELIARVRAILRRTRVAESSRSRTAADEILREGDITINLTSYEVRKGDKLLELTPREFELLRFLMAHAGQVFSREALLEEVWGYDYYGDVRTVDVTVRRLREKLEENSARPEYIKTRRGVGYYFQRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J72</accession>
    <name>Q67J72_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative formate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FNT transporter (TC 2.A.44) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197409.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3297"/>
    <dbReference type="EnsemblBacteria" id="BAD42278">
        <property type="protein sequence ID" value="BAD42278"/>
        <property type="gene ID" value="STH3297"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3297"/>
    <dbReference type="eggNOG" id="COG2116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036896_3_0_9"/>
    <dbReference type="OMA" id="MIWFPIM"/>
    <dbReference type="OrthoDB" id="1834167at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000292">
        <property type="entry name" value="For/NO2_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024002">
        <property type="entry name" value="For/NO2_transpt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30520">
        <property type="entry name" value="PTHR30520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01226">
        <property type="entry name" value="Form_Nir_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01005">
        <property type="entry name" value="FORMATE_NITRITE_TP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="30887" checksum="BD6D068C879FE610" modified="2004-10-11" version="1">MAFASPQETALAVAQANETRASNSIPKLIVLGLLGGAFTGMGAEAATMICHDFTFLGAGLQQFAKGSLFSIGVIFAVICGGELFTGDALLWLAFMDERVGWKKMLKVLGVVLLANVVGATAYAWLYYQSGLFLFNNAELGAEAVRTAVTKVNLTWWQVFVRGVLCNWLICLGVWMAFASKEVISKIASLYIGVMVFVMSAFENSVANFYYLPAGWFAKHIPAVVEAANLGAKLDTLTWSNIITRNWIPSALGNFVGGALFVATAYWFVYVRQAPATPDQSMAASAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J85</accession>
    <name>Q67J85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3284"/>
    <dbReference type="EnsemblBacteria" id="BAD42265">
        <property type="protein sequence ID" value="BAD42265"/>
        <property type="gene ID" value="STH3284"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3284"/>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049311_4_2_9"/>
    <dbReference type="OMA" id="QHKTQAL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023841">
        <property type="entry name" value="BshB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04000">
        <property type="entry name" value="thiol_BshB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="217" mass="24539" checksum="9804AEC5C6F4009A" modified="2004-10-11" version="1">MKEHVLVIMPHPDDETFAVGGTIALYAQRGVPVTYVCGTRGEMGRRMGRPPFATRESLPGLREKELREACRILGITDLRFLGLWDKTVEFEDPEALAARLKAIIEEIRPSLIITYHPDYSVHPDHMALGRATVRAVAQLPPDRRPPVHTRAFGKASAVLGEPDLVVDVRPVWETKLAAIRAHRSQSALVLADDDPEAQERLRRDRTQEAYYVWKFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD1</accession>
    <name>Q67JD1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gate domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197350.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3237"/>
    <dbReference type="EnsemblBacteria" id="BAD42219">
        <property type="protein sequence ID" value="BAD42219"/>
        <property type="gene ID" value="STH3237"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3237"/>
    <dbReference type="eggNOG" id="COG0370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151192_0_0_9"/>
    <dbReference type="OMA" id="MLSFCHS"/>
    <dbReference type="OrthoDB" id="1116727at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="19"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="145" mass="15007" checksum="4EC82EE24353F2E2" modified="2004-10-11" version="1">MDLAASVKQGTRAGLLVLWELAKVIVPAVMLIHVLEQSGWLDRISDWLGPVMGIFGLPGEAALALVSANLSTIYAGLGVTVALGLPARETTILAAMMMINHAAISETALVAKTGARAGWVLLARTVAMVVVALLLNWLLPGAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD3</accession>
    <name>Q67JD3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription-repair-coupling factor</fullName>
            <shortName evidence="9">TRCF</shortName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 13" type="primary">mfd</name>
        <name evidence="13" type="ordered locus">STH3235</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197348.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3235"/>
    <dbReference type="EnsemblBacteria" id="BAD42217">
        <property type="protein sequence ID" value="BAD42217"/>
        <property type="gene ID" value="STH3235"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3235"/>
    <dbReference type="eggNOG" id="COG1197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_2_2_9"/>
    <dbReference type="OMA" id="WAPPCRE"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00969">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="703"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="885"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD42217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1243" mass="138446" checksum="E1EDDBA0805D0E51" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE0</accession>
    <name>Q67JE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3228"/>
    <dbReference type="EnsemblBacteria" id="BAD42210">
        <property type="protein sequence ID" value="BAD42210"/>
        <property type="gene ID" value="STH3228"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3228"/>
    <dbReference type="eggNOG" id="ENOG5032YWW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168343_3_0_9"/>
    <dbReference type="OMA" id="FIEGQIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012504">
        <property type="entry name" value="Spore_YabP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022476">
        <property type="entry name" value="Spore_YabP/YqfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038705">
        <property type="entry name" value="YabP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07873">
        <property type="entry name" value="YabP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02892">
        <property type="entry name" value="spore_yabP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="11145" checksum="9D808777974633FE" modified="2004-10-11" version="1">MEERTHEGATHSLSIANRERVQITGVVAVDSFDDAEVNLETEMGLLTIRGEELQIKQLDLEKGQFEISGFVSALQYASPRQRTGRQQRGRSFLERLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG7</accession>
    <name>Q67JG7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="5 9">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3201</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="5 9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197314.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3201"/>
    <dbReference type="EnsemblBacteria" id="BAD42183">
        <property type="protein sequence ID" value="BAD42183"/>
        <property type="gene ID" value="STH3201"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3201"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_9"/>
    <dbReference type="OMA" id="MTGAMVP"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="8 9" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="10">
        <location>
            <begin position="179"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="415" mass="43498" checksum="05CAC2B15722B1D4" modified="2004-10-11" version="1">MLRVEEACELLLHRAPPRRVERVPLEDALGLVLAEDVHAPEPLPAFARSGMDGYAVRSADTLGATREQPVVLRPVGVIPAGHPREAELAPGTAVAIMTGGAVPPGADAVIRLEEVRVTPEGVLVFRPVPPMENVAPVGEDVREGELILEAGHLIRPQEINLLAALGILEVPVFARPRVGILSTGDELVPAHQTPGPGQIRNSNSPGVAALVRAAGGVPIVLGVARDVTEVIAGMLREAAGCDLILTTGGVSVGRFDVVREALTILGAEQLFWRVSIKPGTPVCASVLEGRLVIGLSGNPAAAITDFDLLVRPLLDHLLGRRRLGLRAAEGVLDQPVLKTAGVTRYLRARAYNGPDGEIRLDTSMAQRAGVLSSMSHANAYAVVPAHAGPLPAGARVRALLLDDADVFVPPPRGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ5</accession>
    <name>Q67JJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3173"/>
    <dbReference type="EnsemblBacteria" id="BAD42155">
        <property type="protein sequence ID" value="BAD42155"/>
        <property type="gene ID" value="STH3173"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3173"/>
    <dbReference type="HOGENOM" id="CLU_1460593_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="185" mass="20918" checksum="ADEDC83D8F3FD004" modified="2004-10-11" version="1">MALALPDWIRGMLLRPAETFAQAQTQMRFAYLWILLTVFTVEAVMLLFHPSVRSAVPSLPAGALLWSLLNLMLTLFAVQAVLLFWSGRIFGWKIELQEAAKYTGLVWVLFLVEDIVTFVPYLTQRDWLVLWASVPFLVWRVVAQTAGVHRISGLPLVRCLGLVLTATLPWNLPLLYLNWSGLVAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX0</accession>
    <name>RS4A_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4 A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsD1</name>
        <name type="synonym">rpsD</name>
        <name type="ordered locus">STH3048</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197163.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JX0"/>
    <dbReference type="SMR" id="Q67JX0"/>
    <dbReference type="STRING" id="292459.STH3048"/>
    <dbReference type="EnsemblBacteria" id="BAD42030">
        <property type="protein sequence ID" value="BAD42030"/>
        <property type="gene ID" value="STH3048"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3048"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_1_9"/>
    <dbReference type="OMA" id="NVVFRMG"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000228933" description="30S ribosomal protein S4 A">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <sequence length="207" mass="23916" checksum="F97E0B4426F83FAD" modified="2004-10-11" version="1">MARYTGPVCRLCRREGVKLYLKGEKCYSDKCPIVKRATPPGQHGASRRKPTEYAIQLREKQKARRYYGVLERQFERYFEMASRKKGVTGEVLLQTLERRLDNVVYRMGFAASRAEARQIVKHSHIEVNGRKVNIPSYLVREGDVVAVRESSREHKRIKELAAAATRTVPAWLSVDPEALRGTVLRLPNRDEIDTPVQEQLIVEFYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ6</accession>
    <name>Q67JZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197136.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3021"/>
    <dbReference type="EnsemblBacteria" id="BAD42004">
        <property type="protein sequence ID" value="BAD42004"/>
        <property type="gene ID" value="STH3021"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3021"/>
    <dbReference type="HOGENOM" id="CLU_1395692_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="20788" checksum="FCC90B8F99A9C934" modified="2004-10-11" version="1">MVKRIGPIPETKPRSWRHRLTAPLSAAARQVAELEEGVGLRLLTERLSAMDLGERSVLVVRPTLGFMTVDLCLVGPGRALVINALHWAGDIVPGPKGEWRGAGGKVDLGRPDRRARVFCERLAFTGRARGIVLEPVVVFTAGAARCQGTEPEATLVQWDELDAFVAGRIPASAVGFPAADLIRLISPPGAGGTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K05</accession>
    <name>Q67K05_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3012"/>
    <dbReference type="EnsemblBacteria" id="BAD41995">
        <property type="protein sequence ID" value="BAD41995"/>
        <property type="gene ID" value="STH3012"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3012"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010448_0_0_9"/>
    <dbReference type="OMA" id="GQGFYKK"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="9">
        <location>
            <begin position="7"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="8">
        <location>
            <begin position="208"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="794" mass="86934" checksum="E827CE7168422ABB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K58</accession>
    <name>Q67K58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197072.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2957"/>
    <dbReference type="EnsemblBacteria" id="BAD41940">
        <property type="protein sequence ID" value="BAD41940"/>
        <property type="gene ID" value="STH2957"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2957"/>
    <dbReference type="eggNOG" id="COG3859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090959_0_0_9"/>
    <dbReference type="OMA" id="VQVLFDY"/>
    <dbReference type="OrthoDB" id="1865311at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015234">
        <property type="term" value="F:thiamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012651">
        <property type="entry name" value="Thia_Transptr_ThiT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09515">
        <property type="entry name" value="Thia_YuaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02357">
        <property type="entry name" value="ECF_ThiT_YuaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="182" mass="19447" checksum="14728EF5EEFDC27F" modified="2004-10-11" version="1">MSDTRVRLRRLVETAMMIAAAVALSYVKVWEMPQGGSVTLGSMVPILLVGLRHGPGWGILAGVLDGLLQFWLDPYFYHPVQVLLDYPIAFGLLGLAGLARGHARWAAAWLGSLAIAGRFAAHVIAGVVFFGQFAPEGQSPLVYSLIYNGSYLLPEAVLSAVLLMVLLPALDRALPAQVGSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K66</accession>
    <name>Q67K66_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41932">
        <property type="protein sequence ID" value="BAD41932"/>
        <property type="gene ID" value="STH2949"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2949"/>
    <dbReference type="HOGENOM" id="CLU_1314845_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="209" mass="22060" checksum="AE79BE7AFDCD272D" modified="2004-10-11" version="1">MPLAVAPALQEQLAPFRPADAEQVWVRLVDTQETDEAFSFQVQVIRRDSAGRLALEGDDEVLVSYLEGGEPLVTGYAHAAAGAPDLAVAVWYGAGPEDAAAPALLDLPAAVLADGAGGYRVSVEALAVLGEIAWQEDGSVSLVLAGGTRTEPLSVAEIEGRSYVQGTDLEAAVDGRRVDLSDAGYTYAVDWRPDLGQLGLWRAVRRPMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC7</accession>
    <name>Q67KC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197005.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2888"/>
    <dbReference type="EnsemblBacteria" id="BAD41871">
        <property type="protein sequence ID" value="BAD41871"/>
        <property type="gene ID" value="STH2888"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2888"/>
    <dbReference type="HOGENOM" id="CLU_2572602_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036473">
        <property type="entry name" value="Mopterin_CF_MoaD-rel_C_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="81" mass="8551" checksum="D733B82EAE7497A2" modified="2004-10-11" version="1">MNLDLRGSTPDQIRGYLESLGGTVRPDGTVAGPDWTATLEAGEHRAFGAVWPRVVVTFSGDPQSVARVVSGLRLRAMRGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM7</accession>
    <name>Q67KM7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2786</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2786"/>
    <dbReference type="ESTHER" id="symth-q67km7">
        <property type="family name" value="Homoserine_transacetylase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41771">
        <property type="protein sequence ID" value="BAD41771"/>
        <property type="gene ID" value="STH2786"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2786"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_1_2_9"/>
    <dbReference type="OMA" id="DVARMQY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="PTHR32268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="48"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" evidence="2 3">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="347" mass="37705" checksum="EC949E9BD0D8CF0E" modified="2004-10-11" version="1">MRAQEATTRVTNTRQLFSTDRLRLEVGETISATVGYETYGRLNARRDNAVLLCHPLAADCHAAAGPADPRPGWWEPLIGPGRAFDTDRYFVIAADTFCGWGTGEAIPQITVRDNVRLQRQLLKSLGIERLACVAGPSTGGFQALEWAVTYPDRVDQVIAVASGHQASPLFALAVCQAAIDAIRTDPAEGLLYALRLYLTLSSSNDWLDAVWGRRTAPASPHPWTGPEGRYAFQAEVEAEAERLAAHLDPGRFAAAARMALLHNIAHGNRDLDVAAQKIAAEILLIPVSSDILFPPSAGREFADLVRTYGGRAEVWVLESLAGHRAALLEAHRLAEPISAFLGHPVWH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ1</accession>
    <name>NRDR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdR</name>
        <name type="ordered locus">STH2761</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196880.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KQ1"/>
    <dbReference type="SMR" id="Q67KQ1"/>
    <dbReference type="STRING" id="292459.STH2761"/>
    <dbReference type="EnsemblBacteria" id="BAD41746">
        <property type="protein sequence ID" value="BAD41746"/>
        <property type="gene ID" value="STH2761"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2761"/>
    <dbReference type="eggNOG" id="COG1327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108412_0_0_9"/>
    <dbReference type="OMA" id="YRFTTYE"/>
    <dbReference type="OrthoDB" id="1551627at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000182366" description="Transcriptional repressor NrdR">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="48"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <sequence length="150" mass="17426" checksum="A7B853D1174EBB12" modified="2004-10-11" version="1">MKCPFCGGESRVLESRPASDEEAVRRRRECLACGRRFTTMERVEVPPLIVVKKDGRREPFNRDKLLTGVLKACEKRPVPMEVIEKLADDIERDLRSSLDREVPSVVIGERVMEALRQVDGVAYVRFASVYREFKDLNEFREQLEQLLKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS9</accession>
    <name>Q67KS9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2733"/>
    <dbReference type="EnsemblBacteria" id="BAD41718">
        <property type="protein sequence ID" value="BAD41718"/>
        <property type="gene ID" value="STH2733"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2733"/>
    <dbReference type="eggNOG" id="COG3296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1585640_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019109">
        <property type="entry name" value="DUF4870"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09685">
        <property type="entry name" value="DUF4870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="168" mass="17602" checksum="16CB02145217CF78" modified="2004-10-11" version="1">MRAAGIGQPSGRRLVRNRLEGMGMSSGTEDRNYGMIAHGLVVLNGASAFMGSLGSLGWAAAVASVVLYFVWKSRSPFVVRHAKQAAGVQVFLFLLSVVLFPFTMLFTVGAAASGSLGGVVALVFLVSLFNLAVGVATIVCGVMGLMRAQKGEEYTYPVVGALVDRIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW2</accession>
    <name>Q67KW2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2699"/>
    <dbReference type="EnsemblBacteria" id="BAD41684">
        <property type="protein sequence ID" value="BAD41684"/>
        <property type="gene ID" value="STH2699"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2699"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_2_0_9"/>
    <dbReference type="OMA" id="FECWGAV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="108"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="485" mass="55509" checksum="DC9C901E8A97BA8B" modified="2004-10-11" version="1">MEGKSQRQVAKALGISRKTVAKYAQLTKVPRYQRQKPAQPVVLTPEFQAEIERRLAENEILPRKQRWTGRTIYEDLVALGYQGSEPTVRRYIAKIRKLKRVQPAFIPLAHDPGESIEVDWGEAVVVLDGAEVTVQILCVRLRWSGMPVVIAYPTQRQEAMFDGLRRALERLSGVPRRMTLDNLKQAVKRILEGRNREEQDAFLSFRTHYLIDSNFCNPASGNEKGSVENLVGLAQRYIVGPHREARTWDELNAILWQRCLEYARRIPQGSTQSILERWEEEKKYLRPLPARPFDCCKVALVSSNKVSCVTFETCRYSVPVQYANRPLQVRAYWDRIDIYAGQEKIASHLRCYERNREILDLDHYLELLHVKPGALEQAKPYRMAQLPPVYHQFRAEMQAQGRSDREFIEILMLHRAYSVSEVSTALELALAQRTACYTAVKQLLAVQPDLGAEASTSTAGELAGIRVQQPSLAEYNRLLKGGVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW5</accession>
    <name>Q67KW5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2696"/>
    <dbReference type="EnsemblBacteria" id="BAD41681">
        <property type="protein sequence ID" value="BAD41681"/>
        <property type="gene ID" value="STH2696"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2696"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107614_0_0_9"/>
    <dbReference type="OMA" id="ANPYVDI"/>
    <dbReference type="OrthoDB" id="1255127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00725">
        <property type="entry name" value="TIGR00725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="194" mass="19860" checksum="7CAA65D282A8B416" modified="2004-10-11" version="1">MLRIGVIGQSGPISEELRAAAFAVGRAVGARGALLFTGGRDGVMAAASQGAQSAGGVTVGILPGDDLREANPYVTVPVTTGLTMVGRSEVLVHAVDACIIVGGGAGTLAEIAVAYLYRKPLVALRGTGGWGDHLASALVDGRFLDHRRLVPIHYVADPEEAVALAAELAQRGQRPPDRPAPGLGDVPKLREASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW9</accession>
    <name>Q67KW9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prephenate dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2692</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2692"/>
    <dbReference type="EnsemblBacteria" id="BAD41677">
        <property type="protein sequence ID" value="BAD41677"/>
        <property type="gene ID" value="STH2692"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2692"/>
    <dbReference type="eggNOG" id="COG0077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_4_2_9"/>
    <dbReference type="OMA" id="HTRFVIL"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="10">
        <location>
            <begin position="207"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="site" description="Essential for prephenate dehydratase activity" evidence="8">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001500-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="290" mass="31724" checksum="046348AB67F5777F" modified="2004-10-11" version="1">MCDRMTEKSSSGVNSMPVVAFQGELGAYGDEAVRARFGPSAEPYPCKSFVDLFEAVASGTVDYGLAPVENSQAGSINDVYDLLRQYDLYVAGEVLHPVNHALLALPGQTLSDIRRVISHPQALAQCDRFLRDLGVEVMATYDTAGAAKMIREQGLTGVAAVAGLGAAQRYGLAVLAESIQTIKDNITRFVVLQRDPAPREEGPQKTMLFLALAHQPGSLYMALGALANRNINLLKLESRPSRNRPWEYVFYLDFEGHRDDPHVRAALADLAKHANYCKVLGSFRRETVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L10</accession>
    <name>Q67L10_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196773.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2651"/>
    <dbReference type="EnsemblBacteria" id="BAD41636">
        <property type="protein sequence ID" value="BAD41636"/>
        <property type="gene ID" value="STH2651"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2651"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_3_2_9"/>
    <dbReference type="OMA" id="YMYAQRT"/>
    <dbReference type="OrthoDB" id="1087129at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="45"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="459" mass="50908" checksum="6D76110E9121F71D" modified="2004-10-11" version="1">MVYNNRAHQADGGGGAVVYREKSFWLETAGEYREQPPLEGERTADVCVVGGGFTGVSAALHLKEKAPGLRVLVLESEVVGFGASGRNAGFCMTLFGLAPEVTRWRFGAARLREADAYMLRAVDLVRRRVAEHGIDCDFEETGLLTVATNPAQRFRLLREMELERKIGLHETEWLEADAVQAQVRSPLFCGGRFDRHCALLNPAKLVRGLARAAEEAGAEICERSPVVEVVPGSRVRLRTPRGTVTAEKVIFATNAYSARLRPLRFRQLPVHTYIVLTEPLAPEQLSAVGWQGRQGIEDGRNLIHYFRLTRDNRLLMGGNDAFYYYGSADDRDRSGAMQQRLQEAVKRIFPMLSGIRFTHHWGGPISATLDLAPLIGRIGPNMFYSVGCMGHGVALCNLNGATLADLALERQTDLTEVFFVDRRALPVPPEPLRFAVAGALLTAMRLQDRWHERGSRRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L33</accession>
    <name>Q67L33_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycine betaine/carnitine/choline ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2628</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2628"/>
    <dbReference type="EnsemblBacteria" id="BAD41613">
        <property type="protein sequence ID" value="BAD41613"/>
        <property type="gene ID" value="STH2628"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2628"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_7_2_9"/>
    <dbReference type="OMA" id="TAYVLQG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="22"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="23100" checksum="5EC99E438A176236" modified="2004-10-11" version="1">MELMQELVVYVNAHWPRLLGLTIQHVKLALLAVLYGVLVGVPVGYLISRRRKLAEPVLWVANALQTIPALALIGFVMLFLGLSPATGIFCLFVYSLMPIIRSTYTALISIDPALIEAATGMGMTRWQILRIVQLPLALAVMMVGIRLALVMSIGTASIMSLAGAGGLGSEIFAGIDRVQDKMILAGALPAALLAIAADLGMGALERWLTPRGLRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L68</accession>
    <name>Q67L68_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196715.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2593"/>
    <dbReference type="EnsemblBacteria" id="BAD41578">
        <property type="protein sequence ID" value="BAD41578"/>
        <property type="gene ID" value="STH2593"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2593"/>
    <dbReference type="eggNOG" id="COG5632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1229360_0_0_9"/>
    <dbReference type="OMA" id="HWTANER"/>
    <dbReference type="OrthoDB" id="1584918at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06583">
        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00644">
        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="24722" checksum="B54CFC7D561D9DB5" modified="2004-10-11" version="1">MIPIIPDMLPEDHPLERLPMTPRYITIHDTGNPAPGANAAMHGRYIRSQAAIEREVMWHFTVDDREIRQHLPLDRNGWHAGDGWYGTGNRQSIAIEICENADGNRAAAEANAAALVAYLIQTVPTLLPFPECVVQHNRWSGKDCPRVLRGRPGGWEAFLSSVAANLNDGRRPIPVRDPAGRLLLTGWLEDGTTVVPLRALAEALGLVVEWDAAAAEARLRWPGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE1</accession>
    <name>Q67LE1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196643.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41505">
        <property type="protein sequence ID" value="BAD41505"/>
        <property type="gene ID" value="STH2520"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2520"/>
    <dbReference type="HOGENOM" id="CLU_139038_1_0_9"/>
    <dbReference type="OrthoDB" id="1876435at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="130" mass="15029" checksum="66414C19194A1B1D" modified="2004-10-11" version="1">MNKAEMYYEYLKEEGYVPRYDSDGDIVFKVEGLTYLLFASEDDEPFFRLCLPNIWKVESAAERERALAAACRVNAEIKVVKLHLVEDNVWASVEMLIDPPEGFKPVFDRALKIVRLGAERFVSYMNTPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH9</accession>
    <name>Q67LH9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2482"/>
    <dbReference type="EnsemblBacteria" id="BAD41467">
        <property type="protein sequence ID" value="BAD41467"/>
        <property type="gene ID" value="STH2482"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2482"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_453354_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269255" evidence="1">
        <location>
            <begin position="35"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="602" mass="63980" checksum="43A88BAABEE1566F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ2</accession>
    <name>Q67LJ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196592.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2469"/>
    <dbReference type="EnsemblBacteria" id="BAD41454">
        <property type="protein sequence ID" value="BAD41454"/>
        <property type="gene ID" value="STH2469"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2469"/>
    <dbReference type="HOGENOM" id="CLU_1844091_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269256" evidence="1">
        <location>
            <begin position="36"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="14195" checksum="F2D715EEA3098BFE" modified="2004-10-11" version="1">MSKKTRVVTSTACPVDVSRPVVSVSCPKTVPTATAGVTDQSDKKGFFDKKGFFPFSFGFGGKSVPGQVFNNPAVDLAPFSKLASLAAFSPAAALAAKSPVLAIFNPAINPAAAFLQFNPGAAPTLALTGKGKPFGKFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM9</accession>
    <name>Q67LM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2432</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196555.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2432"/>
    <dbReference type="EnsemblBacteria" id="BAD41417">
        <property type="protein sequence ID" value="BAD41417"/>
        <property type="gene ID" value="STH2432"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2432"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031960_8_0_9"/>
    <dbReference type="OMA" id="HYAASTM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269527" evidence="1">
        <location>
            <begin position="31"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase2" evidence="2">
        <location>
            <begin position="68"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="301" mass="33473" checksum="5D2C69906B4CA0C4" modified="2004-10-11" version="1">MNGRAWHRRLPWLALGLAGLLALAPVTAAAPGGPSVLRVPDDAADVVRRIERLTRWAPGVVSVWYYDLSTHETFGVRADEVYTAASTVKVLIANYLYHLAATGQVSLDEPLAIRAVDWKDGSGILNGVPDGTRLPLREVARLMLVHSDNTAANALMRRLGVQPMVQYYRSLGIRYGTPRMHTPGELWEGNRIAPEDLGIVLRNIWEAARRSPEPWEELLGFMRESRSKGRIPAGLPSHVPMANKTGSKGTSFHDAALVLDRRPYVLVVMTQGMTARQASHYIAAVSREVWEWHVRRGVRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP9</accession>
    <name>Q67LP9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2412"/>
    <dbReference type="EnsemblBacteria" id="BAD41397">
        <property type="protein sequence ID" value="BAD41397"/>
        <property type="gene ID" value="STH2412"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2412"/>
    <dbReference type="HOGENOM" id="CLU_1805186_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="143" mass="15034" checksum="65B85805F1979E15" modified="2004-10-11" version="1">MPPRGRSTPGAASALFALRVGEGAGGYLSERYLLCSHCVLVRAPADTSPGGICSVRTACWCKRGGVPLGAAQRWGHRLAQPTVTAYRPGASGRGHPRRGWPHGARTDRKPQGRVACRGGPTARANRPNASGRDHPGRGCPHRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR5</accession>
    <name>Q67LR5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2396"/>
    <dbReference type="EnsemblBacteria" id="BAD41381">
        <property type="protein sequence ID" value="BAD41381"/>
        <property type="gene ID" value="STH2396"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2396"/>
    <dbReference type="eggNOG" id="ENOG502ZBRV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1015371_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025576">
        <property type="entry name" value="YwiC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14256">
        <property type="entry name" value="YwiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="28869" checksum="4C26C0876D6B73DF" modified="2004-10-11" version="1">MRLRPGPVPLPREHGAWAMVLTPAVVAVLALGPQPLGLVALLGWLTAYAARGPLEVLLGRGASGRAGMASAEPEVAGFWLLALAAAAAALLLPVAALRPAVLGLLAGALLLACLVFWLAQRGRTRSLLSGFLSVTGLMAGAPLYELAGAGGMSLRGWALTYACFAFFAGSIFRVKTMARERKRRSFHDLSVAVHFAFLAAAAYPAVRGWAPPLVPLALVPPLVWSVVCAWRARRRGAARLDRVGWSEVFLTVLFAGLTVLALRLPAWPPPAAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW2</accession>
    <name>Q67LW2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196472.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2349"/>
    <dbReference type="EnsemblBacteria" id="BAD41334">
        <property type="protein sequence ID" value="BAD41334"/>
        <property type="gene ID" value="STH2349"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2349"/>
    <dbReference type="eggNOG" id="COG2876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062599_1_1_9"/>
    <dbReference type="OMA" id="SMTIQEF"/>
    <dbReference type="OrthoDB" id="687380at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006268">
        <property type="entry name" value="DAHP_syn_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01361">
        <property type="entry name" value="DAHP_synth_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="28958" checksum="681913D35F873019" modified="2004-10-11" version="1">MARPLRVTAEPGRTRPVLIRPGLAVGGGTPVVIAGPCSVETPEQIRAAAQAVRRAGARMLRGGAFKPRTSPYSFQGLGEEGLRLLAQAGQLTGLPVVTEVMDPREIDLVARYADLLQVGARNMQNFRLLTALGEIDKPVLLKRGPGATLEEWLNAAEYVASRGNERIILCERGIRTFETYTRYTLDLASAVAAKQLTHLPVIADPSHACGRRELVPAMALAALAAGLDGVMLEVHPDPARALSDGEQALTPVDLERFMAAAGLLSGTSAADSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY8</accession>
    <name>Q67LY8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4212 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2323</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196446.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2323"/>
    <dbReference type="EnsemblBacteria" id="BAD41308">
        <property type="protein sequence ID" value="BAD41308"/>
        <property type="gene ID" value="STH2323"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2323"/>
    <dbReference type="eggNOG" id="COG4327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140854_4_1_9"/>
    <dbReference type="OMA" id="FYAWRMN"/>
    <dbReference type="OrthoDB" id="2029157at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019886">
        <property type="entry name" value="Na_symporter_ssu_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13937">
        <property type="entry name" value="DUF4212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03647">
        <property type="entry name" value="Na_symport_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4212" evidence="2">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="87" mass="9951" checksum="6DAB7893E1FF976F" modified="2004-10-11" version="1">MSPKDAERYEQHFRANLGLAVKLLLVWFLVSIGAGAVTEWLDQFRILTGFPLGYYMGAQGAQITFVILIFIYAFRMRAIDQKYGVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ0</accession>
    <name>Q67LZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetyl-coenzyme A synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2321</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_006177.1"/>
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    <dbReference type="EnsemblBacteria" id="BAD41306">
        <property type="protein sequence ID" value="BAD41306"/>
        <property type="gene ID" value="STH2321"/>
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    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
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        <property type="entry name" value="ACAS_N"/>
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        <property type="entry name" value="AMP-bd_C"/>
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        <property type="entry name" value="AMP-bd_C_sf"/>
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        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
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    </dbReference>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="EMBL" id="BAD41306.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="632" mass="69570" checksum="26DEC1E44AAB445D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M51</accession>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
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        </location>
    </feature>
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            <end position="526"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="549" mass="61116" checksum="7E5E0FA895BCFE43" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M60</accession>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
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    </dbReference>
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        <source>
            <dbReference type="EMBL" id="BAD41235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="54" mass="5726" checksum="C9D0408526109EAD" modified="2004-10-11" version="1">MDEAAHCDRLAMMNAGRLVAVGTPRELREAYAAGGGLEQVFVHLTRMEGAASPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M90</accession>
    <name>Q67M90_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    </dbReference>
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            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="16905" checksum="03D65EEE68D5F782" modified="2004-10-11" version="1">MLLRSRSRFPNFQSHLTGHPPAHLSPPSPALVTARRLAFSTLVLATVGSGRITQQKDGLGQPNWNWAAPSWDPRWSSTTGGGILWWWLIAQTTPERRTGRKDGPSAPVTTGKSGSYRGWISLTGQFTDRHIKQRRARRLKKGSAASSRHLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK8</accession>
    <name>Q67MK8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2100</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
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            <taxon>Symbiobacteriaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="BAD41085">
        <property type="protein sequence ID" value="BAD41085"/>
        <property type="gene ID" value="STH2100"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2100"/>
    <dbReference type="eggNOG" id="COG3336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054944_0_1_9"/>
    <dbReference type="OMA" id="MKLGYIM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019108">
        <property type="entry name" value="Caa3_assmbl_CtaG-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09678">
        <property type="entry name" value="Caa3_CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="297" mass="33693" checksum="FA092BF888393015" modified="2004-10-11" version="1">MASPKPGCPPCPAATEGGAPMTADLWAHRFEDLWYPDLLAWTVLLNAAYLLLVNLWRRAFNWGPPVPVWRQVLFCLGLWTVYLSEGTPIHIMSELYLFSVHMVQHTLLTMVMPPLILLGTPEWALRPLLRRPWAKRILRTLVHPVPALLLFNLIYSIWHLPVLYQAILLYHWFHAVQHAILVATALLMWWPVCSPTRELPPLSEPGQMVYVFLAGLAQIAAFAVITFADVVLYPFYEEAPRVFGIDPMSDQQLAGVVMHLASGVIFVFAWILIFFRWVAREDRDATPGPAGPDRATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM4</accession>
    <name>Q67MM4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2084</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2084"/>
    <dbReference type="EnsemblBacteria" id="BAD41069">
        <property type="protein sequence ID" value="BAD41069"/>
        <property type="gene ID" value="STH2084"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2084"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_12_2_9"/>
    <dbReference type="OMA" id="ENIVAMW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="7">
        <location>
            <begin position="62"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="6">
        <location>
            <begin position="283"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="234"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="458" mass="52833" checksum="424D22A172B1344E" modified="2004-10-11" version="1">MSNVFIMSKKTYRPYQPNQLLLLPPALQDWLPHDHFAYFLSDVVDSLDLSQIERTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMSYSRMVEEEQRLKAEIAELLQKAEEADQAEDDRFGPHRRGDELPEELRFREQRLAKIQEAKAALEGEARLRAGVDEHEPPEGPEPKGSKGGRRRKQRKAEPAPTAQRNFTDPDSRIMKGSDKQFIQGYNAQAVVDADTQIIVAAAVTNQAADAPHLIPMMEQVEANVDEMPEEVSADAGYFSEQNVQWLLQRRINPYIPPEKQRHSERRRAKSPRGPIPKGATLRDRMRRKLQLKRHAERYRKREQSVEPVFGQIKEARGFRQFHLRGLEAVNGEWVLVCLCHNLLKLYAFRQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM5</accession>
    <name>Q67MM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Molybdopterin oxidoreductase iron-sulfur binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2083</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2083"/>
    <dbReference type="EnsemblBacteria" id="BAD41068">
        <property type="protein sequence ID" value="BAD41068"/>
        <property type="gene ID" value="STH2083"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2083"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_1_0_9"/>
    <dbReference type="OMA" id="CHACTEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="28135" checksum="A9AB1B782BA189BA" modified="2004-10-11" version="1">MQPSAALRGHLPETLARSLHPSPGEVHRSSKGVTPMTAAARDPGSRHEPYWVMVIDLQKCIGCDTCTVSCKAENRTPPGVSYNVVLEMELGEYPNTRRAYVPRPCMQCDSPPCAGVCPVGATYKGESGIVVIDDDRCIGCRYCIAACPYGARSFDFGENYDEMLGYTEVQAPEYGVDRGPRRRGETPIGTVRKCTFCLHRIHRGEEPACVETCIGDARYFGDLNDPNSVVAKLAASPRAFRLKEELGTQPRVIYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS1</accession>
    <name>Q67MS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen fixation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2037"/>
    <dbReference type="EnsemblBacteria" id="BAD41022">
        <property type="protein sequence ID" value="BAD41022"/>
        <property type="gene ID" value="STH2037"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2037"/>
    <dbReference type="eggNOG" id="COG0822">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079283_4_0_9"/>
    <dbReference type="OMA" id="MKLDSMY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="16202" checksum="58E142A024CE0C38" modified="2004-10-11" version="1">MNLSALYQQVILDHYKKPRNKGAVERAVLKKHLHNPTCGDDIEVQVSLGEDGRIDDVKWNGRGCSISMASASMMSVALKGKTLAEAQELMQSFYRMIQGEQGNYKALGEIQALSGVSKFPVRIKCATLAWHCLEEGIREYEGGDANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU1</accession>
    <name>Q67MU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2017"/>
    <dbReference type="EnsemblBacteria" id="BAD41002">
        <property type="protein sequence ID" value="BAD41002"/>
        <property type="gene ID" value="STH2017"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2017"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_15_6_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="17756" checksum="0C7900D37E65442C" modified="2004-10-11" version="1">MNALSPLRTEISRLWHQVNREMRSLLDDAFRQSAMPPPVYFMLREIVREPGITVSELSRRVAMVKSHVSRTVDHMADRGYVRKESDPADQRLIRLYATAPAERELEQTDRRVCAAWQSLVADMSAEELAAVEQGLRILLKTLERNATTRPREKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW3</accession>
    <name>Q67MW3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1995"/>
    <dbReference type="MEROPS" id="S54.027"/>
    <dbReference type="EnsemblBacteria" id="BAD40980">
        <property type="protein sequence ID" value="BAD40980"/>
        <property type="gene ID" value="STH1995"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1995"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055068_5_1_9"/>
    <dbReference type="OMA" id="IAYWAHA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="67"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="25027" checksum="6EFC6E7DADAF19BA" modified="2004-10-11" version="1">MVLPLRDSPAVRRRPWVNWALIALNFAVFLLEMASPVTGEWLIGNFALRPTALLSPDAWAASGGWPLVTLFTSAFLHGSWGHVLGNMLYLWIFGDNLEDRLGHGRYLLFYVLCIALANLAHAVANPTSPIPTIGASGAVAGVLGGYILSFPRARVLALVPIGAFVPAVRVPAWVFLGLWFFVQLASGLAPIWLHDLTQTVAFWAHINGFLSGVALAQLLSPRQEKAEEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N34</accession>
    <name>Q67N34_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1924"/>
    <dbReference type="EnsemblBacteria" id="BAD40909">
        <property type="protein sequence ID" value="BAD40909"/>
        <property type="gene ID" value="STH1924"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1924"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_8_4_9"/>
    <dbReference type="OMA" id="FWADPYA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="187" mass="20587" checksum="A3500729A9433E54" modified="2004-10-11" version="1">MRHGATAANAEGRYVGWEEHPLSADGLAQAEAAARYLSRFRLTGMRTSDLMRCRQTAERIGRATGLTPAPDPRLRELNFGRFSGLTYEEIARQWPEELAAWLADPEHAPPPGGESLASLRSRALAALPRQDGAVVVTHGGVIRTVLAHLTGRGLWDWRVPPGAVVLLRWDGERAVDEPRVWRPEAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N79</accession>
    <name>Q67N79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1879</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1879"/>
    <dbReference type="EnsemblBacteria" id="BAD40864">
        <property type="protein sequence ID" value="BAD40864"/>
        <property type="gene ID" value="STH1879"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1879"/>
    <dbReference type="eggNOG" id="COG1108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028808_3_1_9"/>
    <dbReference type="OMA" id="RIDLMAY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="27651" checksum="408CF0024BFB7752" modified="2004-10-11" version="1">MLSLAFMQRALIAGLVVAVVAPSIGLFLVLRRLALYGDALSHVTLAGVAAGMLAHTYPVATGLLFAVAASLGMDWLRQSYRRYSEMAVAIVLAGALALAVILLSLASGGTGEIMAYLFGSIVTVSPTDVRVIAALGAAVLLCLLLLYKELLATTFDEEYARVGGLPIRGINVAFYVLVALTVAVTMRVVGALLVSSLMVVPVGAALQVARSFRGALALSVAFGVASVLAGLTLAYFLDLAPGGTVVFTAVAILLVILAYKRLRRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA6</accession>
    <name>Q67NA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alkaline-shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1852"/>
    <dbReference type="EnsemblBacteria" id="BAD40837">
        <property type="protein sequence ID" value="BAD40837"/>
        <property type="gene ID" value="STH1852"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1852"/>
    <dbReference type="eggNOG" id="COG1302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113198_4_2_9"/>
    <dbReference type="OMA" id="AYVFLQY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34297">
        <property type="entry name" value="PTHR34297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="13767" checksum="244B7362A3AB7713" modified="2004-10-11" version="1">MKEGKEGEPQVESSIRIADDVVGVIAGIAALEVDGVAGMSGGLAAEVGERMTGKKNPSKGVKVQIGEKEVAIDLYIVVEFGVRIPEVATKVQEAVKRAVESMTGLECVEINIHVQGVSFKEPEDDYRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC4</accession>
    <name>Q67NC4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1834"/>
    <dbReference type="EnsemblBacteria" id="BAD40819">
        <property type="protein sequence ID" value="BAD40819"/>
        <property type="gene ID" value="STH1834"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1834"/>
    <dbReference type="HOGENOM" id="CLU_2774410_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="69" mass="7618" checksum="50F6B384E1DF1C3F" modified="2004-10-11" version="1">MGFPSPPALALWCRPDARAPSRYVCIDLDCKIGFLWRTNCGRQKMRSLFALGDASCSGMITGLVPNRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF0</accession>
    <name>Q67NF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1808</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195936.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1808"/>
    <dbReference type="EnsemblBacteria" id="BAD40793">
        <property type="protein sequence ID" value="BAD40793"/>
        <property type="gene ID" value="STH1808"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1808"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_9"/>
    <dbReference type="OMA" id="MGSNMIT"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="308"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="402" mass="43438" checksum="EA03C5B777CD10E2" modified="2004-10-11" version="1">MNLLETFSVALSALWANKLRSALTMLGLIIGVGSVIAIIAIGRGTQKAVAAELDALNGGVFQLMATPGWSEDGQPTARLRMFRDDDIALLKEALPEIDLIDPQVGMSGRITFGREVEWWDWIRGAGADTAALMNIQLSAGRWFTEEEIARGDRVMVLSASRVEYILGEGVNPLGIELMVNGYPFQIIGVIAPNTGMLSQLLGGQGGDFYVPRSYITRVTGQNSYDHLTVRVRDGYDPVEVKDLAVRVMERLYPGTGYEAYIWTDFMGEFNQVISIVTGVMAAIAGISLVVGGVGIMNIMMVSVTERTREIGLRKAIGARRRDILMQFLVEALTLCLIGGGIGIVLAAGPVTIAARYLETPLSLDLYAIALALGFSAAVGLIFGVYPAVKAARMDPIEALRYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK4</accession>
    <name>Q67NK4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Group II intron-encoding maturase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1754"/>
    <dbReference type="EnsemblBacteria" id="BAD40739">
        <property type="protein sequence ID" value="BAD40739"/>
        <property type="gene ID" value="STH1754"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1754"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_1_1_9"/>
    <dbReference type="OMA" id="RIANCKQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIIM" evidence="2">
        <location>
            <begin position="67"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="24031" checksum="28AB4C2C9D4D031B" modified="2004-10-11" version="1">MTATSTSAANGRENGSTGACATSCRSGYGLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRCLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL1</accession>
    <name>MIAA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="1">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="1">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="1">IPP transferase</shortName>
            <shortName evidence="1">IPPT</shortName>
            <shortName evidence="1">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaA</name>
        <name type="ordered locus">STH1747</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195875.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NL1"/>
    <dbReference type="SMR" id="Q67NL1"/>
    <dbReference type="STRING" id="292459.STH1747"/>
    <dbReference type="EnsemblBacteria" id="BAD40732">
        <property type="protein sequence ID" value="BAD40732"/>
        <property type="gene ID" value="STH1747"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1747"/>
    <dbReference type="eggNOG" id="COG0324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_0_1_9"/>
    <dbReference type="OMA" id="YAKRQYT"/>
    <dbReference type="OrthoDB" id="1069591at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000163991" description="tRNA dimethylallyltransferase">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <sequence length="319" mass="36285" checksum="7973A9B34BAA580A" modified="2004-10-11" version="1">MKLPLVVLVGPTAVGKTALSVAVAQAVGAEIISGDSMQVYRGMDIGTAKIRPEEMGGVPHHLIDIKDPDEEFSVAEFQARVDALIPQICARGRLPMLVGGTGLYVRAVVEKYTFTPMEADHELRARLRQEEERHGPGYLHARLREVDPASAARLHPNDLLRIVRALEVYEQTGVPISATQTAAFSEPRYDDLMIGLTMDRAQLYARIDERVDAMLAAGWLDEVRGLLVRYPPHVRAMQALGYRELVLYLRGLLTWEEAVALIKRNTRRFAKRQFTWFRKERRLTWLDLTGPEARNRATEEIVRLIREKWPCRERKGRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN0</accession>
    <name>Q67NN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1728"/>
    <dbReference type="EnsemblBacteria" id="BAD40713">
        <property type="protein sequence ID" value="BAD40713"/>
        <property type="gene ID" value="STH1728"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1728"/>
    <dbReference type="eggNOG" id="ENOG5032ZQ2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_943109_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012452">
        <property type="entry name" value="DUF1657"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07870">
        <property type="entry name" value="DUF1657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="30871" checksum="59959954ABB569E3" modified="2004-10-11" version="1">MGLPFPVGEGGVQEEDSGMTVHGDLERAIAMAQAARGNYLLFATQSEDEKAHQVFKQMAEDMERHVMILESRLQYLEQYNALVQHSRGQQQGQDQGSGQGQRKAQGQGQTHGQGPGLHQAQGANQPPGQGSGQGQGQGADQREGPQGMGWQFPRMDGQGGGQQAQGQRQDGQQQAQQDVPQQQVGGHGGAPHGQGGPWQGGTGQSGTGQGRWVQQAQGGGENPAGDGQAGGSGQFSQAGQPDPSAGAGTDQQMASQCGQPQSDRQQGQQQSGQDGQPWQQQGQPSQGAEPWQQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN8</accession>
    <name>Q67NN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195848.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1720"/>
    <dbReference type="EnsemblBacteria" id="BAD40705">
        <property type="protein sequence ID" value="BAD40705"/>
        <property type="gene ID" value="STH1720"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1720"/>
    <dbReference type="eggNOG" id="ENOG5032ZFY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169738_0_2_9"/>
    <dbReference type="OMA" id="TSRECGH"/>
    <dbReference type="OrthoDB" id="2064087at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8571" checksum="E55DB487D6C9F298" modified="2004-10-11" version="1">MAQFTPTRRVLPHELLDRLKWEVAEQAGLTEQIVQHGWPQMSSRACGHIGGRIGGRMVKVMLKYAEQALAEGSATLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR3</accession>
    <name>Q67NR3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1695"/>
    <dbReference type="EnsemblBacteria" id="BAD40680">
        <property type="protein sequence ID" value="BAD40680"/>
        <property type="gene ID" value="STH1695"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1695"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1061426_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="208"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="262" mass="28050" checksum="F68B39E20F6AC7F5" modified="2004-10-11" version="1">MTEHGTHGEGGMEQQYHRRWRGGGLGRLLMVATGDVDLDGAPEIVACAGRELKVYRWTGDTYALQAEAALPKDGLSLAVGQVDPGGPGTVAVGTRDNVLLYALTPNGLVALCQTLLYPNAYFRSLSLDDVNDDGRAEVIAGASGAQTIYLYQVMAVGRESRLEELGRLYVGGLVAARPTPSGELVAATKDGWVDVYVPCALLPRETQTIYTVRRGDSLWRLARKFGTSPGTIAKANKLTEPYQLTPGQVLIIPGRRAPKGDP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX5</accession>
    <name>RIMO_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein S12 methylthiotransferase RimO</fullName>
            <shortName evidence="1">S12 MTTase</shortName>
            <shortName evidence="1">S12 methylthiotransferase</shortName>
            <ecNumber evidence="1">2.8.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S12 (aspartate-C(3))-methylthiotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosome maturation factor RimO</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimO</name>
        <name type="ordered locus">STH1633</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37087"/>
            <dbReference type="Rhea" id="RHEA-COMP:10460"/>
            <dbReference type="Rhea" id="RHEA-COMP:10461"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:73599"/>
            <dbReference type="EC" id="2.8.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. RimO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195762.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NX5"/>
    <dbReference type="SMR" id="Q67NX5"/>
    <dbReference type="STRING" id="292459.STH1633"/>
    <dbReference type="PRIDE" id="Q67NX5"/>
    <dbReference type="EnsemblBacteria" id="BAD40618">
        <property type="protein sequence ID" value="BAD40618"/>
        <property type="gene ID" value="STH1633"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1633"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_0_1_9"/>
    <dbReference type="OMA" id="HYAYPTG"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103039">
        <property type="term" value="F:protein methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018339">
        <property type="term" value="P:peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01865">
        <property type="entry name" value="MTTase_RimO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005840">
        <property type="entry name" value="Ribosomal_S12_MeSTrfase_RimO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041582">
        <property type="entry name" value="RimO_TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43837">
        <property type="entry name" value="PTHR43837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18693">
        <property type="entry name" value="TRAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00274">
        <property type="entry name" value="ribosomal_protein_S12_methylth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01125">
        <property type="entry name" value="TIGR01125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000375028" description="Ribosomal protein S12 methylthiotransferase RimO">
        <location>
            <begin position="1"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="158"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="390"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="485" mass="54094" checksum="EC35B4145451696D" modified="2004-10-11" version="1">MTDRPTTALPGAQIAGRPVKVGFISLGCAKNLVDTESMIGLLRNTGYQITNRAEEADVLVVNTCGFIDAAKQESVDAILEAAQHKTRGRCQALVVAGCMVPRYGEELAREIPEIDALVGTADYPRIGEVVAGILAGQRVQQISDPDSITDWNFERVLATPGYTAYLKIAEGCDCACAFCSIPLMRGRHRSRPIESIVDEARRLAGMGVRELVVISQDTTYYGLDLYRKPMLARLLRELAQVDGIRWIRIHYSYPTRITDELIEVIVTEPKVLNYLDLPLQHGSNRVLRIMNRPANAEGYLRLVQKLRERVPDICLRSTFIAGHPGETEEDFELLLDFLRACEFDHVGVFAYSQEEGTKAGQMEQLPEEVRLARRDRAMEVQQEIARRRNQLQVGRELEVLVEGRSPQGRGWFVGRCYGQSPGIDGVVLFRAPAGAELKPGDMVQVRITGVQDYDLLGEATEPLTVDGIAQEQDLILPVFHLTRHQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-12-15" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P12</accession>
    <name>NUOK1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit K 1</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit K 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit K 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoK1</name>
        <name type="ordered locus">STH1596</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195725.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P12"/>
    <dbReference type="SMR" id="Q67P12"/>
    <dbReference type="STRING" id="292459.STH1596"/>
    <dbReference type="EnsemblBacteria" id="BAD40581">
        <property type="protein sequence ID" value="BAD40581"/>
        <property type="gene ID" value="STH1596"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1596"/>
    <dbReference type="eggNOG" id="COG0713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_0_0_9"/>
    <dbReference type="OMA" id="FDVWLSR"/>
    <dbReference type="OrthoDB" id="2081663at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="PTHR11434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000390258" description="NADH-quinone oxidoreductase subunit K 1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01456"/>
        </source>
    </evidence>
    <sequence length="102" mass="10902" checksum="32FD2F872A668CCF" modified="2004-10-11" version="1">MPAFSLNAYVALSAVLFALGGIGVLVRRSPLAILMCIELMLNAANLLFVAFGRAHGGYEGQIMAFLVITVAAAEVAIGLALTVLLFRRRAEVDVDRVNELKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P64</accession>
    <name>DAPB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">STH1544</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195674.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P64"/>
    <dbReference type="SMR" id="Q67P64"/>
    <dbReference type="STRING" id="292459.STH1544"/>
    <dbReference type="EnsemblBacteria" id="BAD40529">
        <property type="protein sequence ID" value="BAD40529"/>
        <property type="gene ID" value="STH1544"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1544"/>
    <dbReference type="eggNOG" id="COG0289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_0_1_9"/>
    <dbReference type="OMA" id="HHPNKAD"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228395" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="167"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="254" mass="27042" checksum="B8C6D8ECF0F8A11C" modified="2004-10-11" version="1">MQPIRVVLAGATGKVGQVLARALVQEPGFALTGAIARQGGGRNLAELVPLGGRPGPTVHGSLEEFLAAGGEADVLVDFSVAEAGRRTIPAAIEASIAPVVGTTGFQPGETETWAAMCRKRGLGGAFIANYAVGIMLLMRFAEEAHRFFPDVEIIEMHHKTKLDAPSGTALRTKARLERGRGDLAAAEVPVHSVRLPGLVAHQEVIFGGLGQTLTIRHDAPSREAYVPGVLMTCRWVLREKRVAFDLEEVAFPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P75</accession>
    <name>Q67P75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative dUTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1533</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1533"/>
    <dbReference type="EnsemblBacteria" id="BAD40518">
        <property type="protein sequence ID" value="BAD40518"/>
        <property type="gene ID" value="STH1533"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1533"/>
    <dbReference type="eggNOG" id="COG0756">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068508_0_0_9"/>
    <dbReference type="OMA" id="YAAFVHP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="PTHR11241"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="4">
        <location>
            <begin position="9"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="14645" checksum="7259CC4F7FE98939" modified="2004-10-11" version="1">MRGYEGRVRLPQRKTRLSAGYDLEAAEEVTIPPGQVALVPTGLKAYMGEDEVLLLAVRSSLAVKRRLALANGIGVIDADYADNPDNEGHILVALVNLGTEPATIACGERVAQGIFVRYLTVAGDQPGGLRTGGIGSTGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH7</accession>
    <name>Q67PH7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195561.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1431"/>
    <dbReference type="EnsemblBacteria" id="BAD40416">
        <property type="protein sequence ID" value="BAD40416"/>
        <property type="gene ID" value="STH1431"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1431"/>
    <dbReference type="eggNOG" id="ENOG5032YCI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169738_2_1_9"/>
    <dbReference type="OMA" id="KENNDHY"/>
    <dbReference type="OrthoDB" id="2064087at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="79" mass="8376" checksum="12DD6AE8CEB234CD" modified="2004-10-11" version="1">MASNNQSSNNSILVSQARAALEQMKNETAAELGIQNYAQQYKGDLPSRVNGSVGGYMVKKMIALAEQQLAGGTGTTTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ6</accession>
    <name>Q67PJ6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="11">IGPS</shortName>
            <ecNumber evidence="4 11">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpC</name>
        <name evidence="13" type="ordered locus">STH1412</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4 11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1412"/>
    <dbReference type="EnsemblBacteria" id="BAD40397">
        <property type="protein sequence ID" value="BAD40397"/>
        <property type="gene ID" value="STH1412"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1412"/>
    <dbReference type="eggNOG" id="COG0134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_2_0_9"/>
    <dbReference type="OMA" id="RGPHDLI"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_B">
        <property type="entry name" value="IGPS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="IGPS" evidence="12">
        <location>
            <begin position="3"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="28202" checksum="70F0984150C851A3" modified="2004-10-11" version="1">MAAARRARPLADVIAAARAAPPPRGFLRALGGPPSGAGAGGTEVRVIAEVKKASPSKGVIRPDFDPVAIARAYAEGGAACLSVLTDERYFQGSLAYLAAIREAVALPLLRKDFIIEEYQVHEARAAGADAILLIVAAFCGDCAGGRTPEDLNRLAALARELGMDVLTEVHTADELALAVASGAPLIGINNRDLRTFHTTLEVTERLAPLVPPDRLLVSESGIWTHDDLRRVAAAGARAVLVGESLMRQPDVAAALRALRGSPEGSVPDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM8</accession>
    <name>Q67PM8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetylmuramoyl-L-alanine amidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195510.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1380"/>
    <dbReference type="EnsemblBacteria" id="BAD40365">
        <property type="protein sequence ID" value="BAD40365"/>
        <property type="gene ID" value="STH1380"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1380"/>
    <dbReference type="eggNOG" id="COG5632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_660432_0_0_9"/>
    <dbReference type="OrthoDB" id="1184688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01510">
        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55846">
        <property type="entry name" value="SSF55846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYZ2" evidence="3">
        <location>
            <begin position="35"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="2">
        <location>
            <begin position="226"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="416" mass="45208" checksum="08060802A3767EFE" modified="2004-10-11" version="1">MTWNAPIMGRSLARPEQMTAYALRDNPFAPDLAGLYLGLASRLGVRGDVAYAQALVETNGFRMDGWAERGRYNFGGLGVTRPGDPGISFRSPEEGVLAHLQALSRQAGGGPLPPDMPDLTPFLSPEDRGRIRRVGDLRRPGWPEDEEYGEAVARTLAAILLEPAEGEPYRIEQDLLPPGSPARPGARDGTGRWQGGGGIVIYRSASPHLDGPGMRALLERPVAGEVRSFHFVIDGRSIRQLVPLGEIAYHTDGRNHRDIGVMICERGWGTSEWEEGYRRLVWLAARLMVVLGIGIEGLSGGFWWNPVRHPYDPTHLGWKPEDGPATGLFHWNRLVADVAAARPAVEKLFGLHPAEPQVSPAAQGRNPPAGDRPGTATQGRNLSIGHTGAAGGGKPPRGNWVQAARTRSARYSSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS2</accession>
    <name>Q67PS2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1336</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195467.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1336"/>
    <dbReference type="EnsemblBacteria" id="BAD40321">
        <property type="protein sequence ID" value="BAD40321"/>
        <property type="gene ID" value="STH1336"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1336"/>
    <dbReference type="HOGENOM" id="CLU_681393_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269603" evidence="2">
        <location>
            <begin position="32"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="39060" checksum="5742516BE53D02FE" modified="2004-10-11" version="1">MKRMPLKWLGLAAAAALLASGCTAQPQNANRVNQSNLFTTTGDADTCAAALGNTVGANAFWGMAGVNRNVTANGVIIGNVALVALPRESQNTPVNGAATGTGGQNGAAAGNGVQTGAAGGNGVPMGTTGNGVGTGTAAGNGLSTGVQRDGDVTGNLGTLTPPGTVDTTPGHVGTVPGNTGSTVMTPGLGTTRGATGVVGGRVESPSLPGTGTTTGTGTRAGTGTRAGTGTPAGTGTAAGTGTMAGSGTRGGTGATTRAGTAFGTRTGTTAADPLERVRTACPTLADIRVVNDETDRARLAEITAAVRSGRPVTEFMSELATMAQRATSAGPGASGRQRNRPENFRNRLQQGNLVPGRAGTPTTPAPAAPRTPPAPNAPGTDGRGSTPARPGTGTATDPGTSATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS3</accession>
    <name>Q67PS3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cation-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1335</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195466.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1335"/>
    <dbReference type="EnsemblBacteria" id="BAD40320">
        <property type="protein sequence ID" value="BAD40320"/>
        <property type="gene ID" value="STH1335"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1335"/>
    <dbReference type="eggNOG" id="COG0474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_1_0_9"/>
    <dbReference type="OMA" id="PLWNNMM"/>
    <dbReference type="OrthoDB" id="178322at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="735"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="762"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="852"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="920"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="9">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="959" mass="103368" checksum="6A8840899CC48FEA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ4</accession>
    <name>Q67PZ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195395.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40249">
        <property type="protein sequence ID" value="BAD40249"/>
        <property type="gene ID" value="STH1264"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1264"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048546_1_0_9"/>
    <dbReference type="OrthoDB" id="1667004at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="29501" checksum="76CD8CAC075A2E82" modified="2004-10-11" version="1">MIELLAESLPLRRVASRLNISVSTAFRWRHRALTVLSARDRKPLSGDVRVETFLVKYSEKGSRVCHGPGSWGYWNVVRKGEEPVGRVRSGQSAGRRRFRLLIDGRPLHVMVAETDTGYEFDILGQGRRNAEMVAAGLARLIRPGSRVFAFGGSEYRRACETLGCEHHDGVAAVSRWFQCLRGAEEGGASRGAEEVTTPEVRFPNLPIWWLRRFRGVATKYLGHYLAWFRDIVRIVEFPAGANMASADGRALLRRPADWGVPPT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q27</accession>
    <name>SYI_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">STH1231</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195362.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q27"/>
    <dbReference type="SMR" id="Q67Q27"/>
    <dbReference type="STRING" id="292459.STH1231"/>
    <dbReference type="EnsemblBacteria" id="BAD40216">
        <property type="protein sequence ID" value="BAD40216"/>
        <property type="gene ID" value="STH1231"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1231"/>
    <dbReference type="eggNOG" id="COG0060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_7_1_9"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098488" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="601"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>L-isoleucyl-5'-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:178002"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="904"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="907"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="923"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="926"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02002"/>
        </source>
    </evidence>
    <sequence length="938" mass="106189" checksum="A21A5E420CA8C381" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q37</accession>
    <name>Q67Q37_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">spoIIGB</name>
        <name evidence="9" type="ordered locus">STH1221</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the sigma-70 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1221"/>
    <dbReference type="EnsemblBacteria" id="BAD40206">
        <property type="protein sequence ID" value="BAD40206"/>
        <property type="gene ID" value="STH1221"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1221"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_7_9"/>
    <dbReference type="OMA" id="EPLNTDW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014200">
        <property type="entry name" value="RNA_pol_sigma-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02835">
        <property type="entry name" value="spore_sigmaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0749">Sporulation</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="8">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="28973" checksum="5B2FA1D6FC670291" modified="2004-10-11" version="1">MYTFVRRQILALQGALFRVSTRVLRWLAARGLVRPRAIYYVGSSEALPPPLTGEEEAALLQRLTQGDESVKNTLIERNLRLVVYIARKFDNTGIWVEDLVSIGTIGLIKAVKTFDPSKRIKLATYASKCIENEILMYLRRASRTRSEVSFDEPLNKDWEGNELLLSDVMGTDSDITYRGLEEEVDRMLLRKAMAKLTGREKRIMELRFGLVDGRERTQKEVADLLGISQSYISRLEKRIFKRLKKEILRME</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q82</accession>
    <name>Q67Q82_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40161">
        <property type="protein sequence ID" value="BAD40161"/>
        <property type="gene ID" value="STH1176"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1176"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1721431_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="17345" checksum="81C491FB2CFA6900" modified="2004-10-11" version="1">MVRRMASRLEQQCERYLRSAYGLSWPQFQVLRELSVERPTMLQRVNDAVYCTRGNLAAIIDRLERDGWLERFRLQGDRRVVMLSLTEKGAQVTAIEAELRARIREWLEPVWREAQEIQEYLGVLLRDMTDPLAAEEALKPKERLVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB8</accession>
    <name>Q67QB8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multidrug efflux protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195271.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1140"/>
    <dbReference type="EnsemblBacteria" id="BAD40125">
        <property type="protein sequence ID" value="BAD40125"/>
        <property type="gene ID" value="STH1140"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1140"/>
    <dbReference type="HOGENOM" id="CLU_1517178_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="177" mass="17797" checksum="C3AA0AA726C633F7" modified="2004-10-11" version="1">MIAFAWVYEAAASMVSQFGQVYLPALGLSMLGAGVVFSASRLLGAAGGWVAARLQGGGADRWLRWGPLGQAALILLAGLARTAGGGVALAVNEGVDGLVYPLLSARINQAVPSAQRATILSFQSLGSSLLIALAFPAAAALPSVFTVYAIVGGALAAAALGWALPRQRAAVPAPEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD5</accession>
    <name>Q67QD5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195255.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1123"/>
    <dbReference type="EnsemblBacteria" id="BAD40108">
        <property type="protein sequence ID" value="BAD40108"/>
        <property type="gene ID" value="STH1123"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1123"/>
    <dbReference type="eggNOG" id="ENOG5031XUS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067338_2_0_9"/>
    <dbReference type="OMA" id="GIIYYIP"/>
    <dbReference type="OrthoDB" id="1637742at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014197">
        <property type="entry name" value="Sporulation_prot_YunB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09560">
        <property type="entry name" value="Spore_YunB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02832">
        <property type="entry name" value="spo_yunB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="28115" checksum="15EF65683EFCDB12" modified="2004-10-11" version="1">MRRFSRSRPLRGMGRGPVRLRFRRGPRLRLRGGLRAWLGRLSLRLRVRRIWLRNLILAAAVLYLLIRIGDLLLMQPLKAVAEVEARRLGAAAVNRVVAAQVSKSLAGAQVVEYVRDESGRIAAYHVNTPLVNRVASEAAVAVQEELKRLAETPVRLPLGALTGSALLSNLGPRLSVQLVPVGSVSITVQQEFKGEGINQTRHRVWLEAAATMRIILPLTTQEVPLVQELPLADTVIVGPVPNALYGGSLGGVTLPAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF1</accession>
    <name>Q67QF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1107"/>
    <dbReference type="EnsemblBacteria" id="BAD40092">
        <property type="protein sequence ID" value="BAD40092"/>
        <property type="gene ID" value="STH1107"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1107"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_474601_0_0_9"/>
    <dbReference type="OMA" id="WMREHAL"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="309"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="594" mass="65864" checksum="12E3DECA7DBB48B0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN6</accession>
    <name>Q67QN6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1022</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195154.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1022"/>
    <dbReference type="EnsemblBacteria" id="BAD40007">
        <property type="protein sequence ID" value="BAD40007"/>
        <property type="gene ID" value="STH1022"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1022"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713565_0_0_9"/>
    <dbReference type="OMA" id="CFRETIT"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="286"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="387" mass="42639" checksum="A512D5E90C779219" modified="2004-10-11" version="1">MRWSDVPVENKAQIAWGALVVVYLLGAQRPELIRPPVVAVFLLSAAAAFLELWLRSRGWPHLLWYDCIVWSALLTGMVVVTGGRGSEVWAAYILMSLTAPVVLRRVAPYILLGVNVTAYGLIYLLYNPFGAPLDWGLLFLRIGTIFLVAYVVDRSTARERQSHARAVALARSRVSELVQARDAERRRIAHDIHDWLGTGIIAPLRRLEIAARQSDVESCRRHVEEAADSLRRAHAELRRLMENLHPHLLEQMGLAEALRAYLTDWGEEHGVAVHYHLTPGPEPPADAALALYRIQQEALNNCAKHADASQVWVTLELGADRVRLTLRDDGHGRPGRPGRGITGMQERAAAFGGTVTVHGQPGRGTTVTAELPLRAAAAAAPAPTEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT2</accession>
    <name>Q67QT2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH976</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH976"/>
    <dbReference type="EnsemblBacteria" id="BAD39961">
        <property type="protein sequence ID" value="BAD39961"/>
        <property type="gene ID" value="STH976"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH976"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="ALINCPK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="26235" checksum="C54AF48E894C53C3" modified="2004-10-11" version="1">MDITKVYKMGSSEVHALRGVSLEIQAGELVAIMGPSGSGKSTLMNILGCLDRPTSGTYELDGRQITARDESELARVRNRQIGFVFQTFNLLPRMSALRNVEQPLLYAGVGARERRRRAREALERVGLGERIHHRPNELSGGQRQRVAIARALVTQPSIILADEPTGNLDSRTGEEILALFQELHAEGATVLIVTHEPDVAQHVNRVIRLRDGRIVSDEPVPNPVRAVDRLAEMGGPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV0</accession>
    <name>Q67QV0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Putative hemolysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH958</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195090.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH958"/>
    <dbReference type="EnsemblBacteria" id="BAD39943">
        <property type="protein sequence ID" value="BAD39943"/>
        <property type="gene ID" value="STH958"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH958"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_9"/>
    <dbReference type="OMA" id="ILTPRTQ"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="13">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="217"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="282"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="415"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD39943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="452" mass="48782" checksum="A80812F7E2DA6388" modified="2004-10-11" version="1">MQHWRALGLILGLVLINAFFAAGEIAVVSARPVRIRQLAEQGNPSAWALLQLMKDQSRFLATIQVGITLAGFLASASAAVGLSAGLGAALQRLGVSPAVAGSAAVVAVTLLLSYLTLVLGELVPKRIALQSPERVALLVARPVLLIARLTRPFVALLTASTNLVVRLLGGQAQPAEPGISEEELRLYVAENTGLQEAEKRMIAGVFSFGDRSVRQVMVPRTEMACLHQDAPLPEALAEVRRHGFWRYPVYREDYDDIVGMVTVKDLLRHADPQAGLRTVADVMRPAWFVPESKQALSLLKEMQAADEQLAVVVDEYGGVSGLVTMEDLLEEIIGEIAEEPSPPSAEPRELELDGGEPVDEVAEQLHIPIPRSPHYETLAGYILHHLGAIPSEGDELAVSGWRLRVVRMDGHRIDRVRAVPDAPDAPGASDRPEPPERQGMRDRASPSGPPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW0</accession>
    <name>Q67QW0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH948</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195080.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH948"/>
    <dbReference type="EnsemblBacteria" id="BAD39933">
        <property type="protein sequence ID" value="BAD39933"/>
        <property type="gene ID" value="STH948"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH948"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="FNEMARN"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="208"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="308"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="622" mass="66023" checksum="2A6CCA207BE73300" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX2</accession>
    <name>Q67QX2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH936"/>
    <dbReference type="EnsemblBacteria" id="BAD39921">
        <property type="protein sequence ID" value="BAD39921"/>
        <property type="gene ID" value="STH936"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH936"/>
    <dbReference type="eggNOG" id="COG2318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1377506_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="1">
        <location>
            <begin position="65"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="23362" checksum="1527C1BEBF354A5B" modified="2004-10-11" version="1">MNGCRRAAIRWAGRRDFGRPGKSIGLEERGHHPAARAVAACRGGNPMLAFDLYRHWETDLRPFTVNALRRMTPEQLAWKPPGWHSSAFELAEHMALCEWVWIYRNALKVVPWEARWESGRFPDLDSLLQYWDEIHRVTVQWLKDTPISQLGRKLPMPYPDMPWATMDWVIYHVMEHEIHHRGQIFMLMRMQGIDPPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ0</accession>
    <name>Q67QZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nitrate reductase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">napD</name>
        <name evidence="4" type="ordered locus">STH918</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195050.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH918"/>
    <dbReference type="EnsemblBacteria" id="BAD39903">
        <property type="protein sequence ID" value="BAD39903"/>
        <property type="gene ID" value="STH918"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH918"/>
    <dbReference type="eggNOG" id="COG3062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2572601_0_0_9"/>
    <dbReference type="OrthoDB" id="1976760at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005623">
        <property type="entry name" value="Chaperone_NapD_NO3_reduct"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03927">
        <property type="entry name" value="NapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="81" mass="8463" checksum="CE069305201CA804" modified="2004-10-11" version="1">MVISGLVFQCDPARVEAVAARLAGVPGLSDITPGGRPGYLVGVLESPGTEASVRTLDHLRAIPGVYSVLPAYIQSVEEDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R52</accession>
    <name>Q67R52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH856</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH856"/>
    <dbReference type="EnsemblBacteria" id="BAD39841">
        <property type="protein sequence ID" value="BAD39841"/>
        <property type="gene ID" value="STH856"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH856"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="WSNEDEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="21"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="205" mass="22195" checksum="86FF41627F47CB01" modified="2004-10-11" version="1">MVAVAPLSGRIARGEFVMITGRSGAGKSTLLALMGGLTRPDGGRVTVMGQDLWALSDAERARFRARHIGFVFQFASLIPTLTVLENLLIPASFLPPGEGGDRKRALELLERVGLPDCADRLPWQLSGGQQKRVAVARALLNRPEVLLADEPTADLDEETERAIVELFVEENRAGTTVVMVTHNKDLVPFASRHLILNAGQVHLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R84</accession>
    <name>Q67R84_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH824</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194957.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH824"/>
    <dbReference type="EnsemblBacteria" id="BAD39809">
        <property type="protein sequence ID" value="BAD39809"/>
        <property type="gene ID" value="STH824"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH824"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="EHDMRFI"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="28463" checksum="561CD44FC76421C3" modified="2004-10-11" version="1">MALLECRDVSRHFGGLKALVDFDLQLEKGELVGLIGPNGAGKTTVFNVITGVFPPTAGEVVLEGRVLNRLKPYQITRLGIARTFQNIRLFKQMTVLENVMTVMHPRCGYGLLDTFLRTPRFLRQERALREEAEALLERMNLADRRHEKAGSLPYGAQRRLEIARALATRPKVLLLDEPAAGMNPSEVGQLVELIRQVKAEFDLTVLLIEHQMGLVMNLCERLVVLDFGQVIARGTPAEIQADPRVLEAYLGKAVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R98</accession>
    <name>Q67R98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194943.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH810"/>
    <dbReference type="EnsemblBacteria" id="BAD39795">
        <property type="protein sequence ID" value="BAD39795"/>
        <property type="gene ID" value="STH810"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH810"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006325_0_0_9"/>
    <dbReference type="OMA" id="CWERAYR"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15831">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="809"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="809"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1080" mass="120806" checksum="43DEA570527BDA3F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE3</accession>
    <name>Q67RE3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194898.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH765"/>
    <dbReference type="EnsemblBacteria" id="BAD39750">
        <property type="protein sequence ID" value="BAD39750"/>
        <property type="gene ID" value="STH765"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH765"/>
    <dbReference type="HOGENOM" id="CLU_2669739_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="8683" checksum="759E95E8D603DBE4" modified="2004-10-11" version="1">MKEVLQWPNWAILATFIAVVISLVLNIVLYQRVQVLYSKFGAQALPFRNRDELEAAFQAGTIDRAEYEQLKRKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI9</accession>
    <name>Q67RI9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194852.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH719"/>
    <dbReference type="EnsemblBacteria" id="BAD39704">
        <property type="protein sequence ID" value="BAD39704"/>
        <property type="gene ID" value="STH719"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH719"/>
    <dbReference type="eggNOG" id="COG2718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049702_2_0_9"/>
    <dbReference type="OMA" id="QYFAYIE"/>
    <dbReference type="OrthoDB" id="481720at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014230">
        <property type="entry name" value="Spore_YhbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006698">
        <property type="entry name" value="UPF0229"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30510">
        <property type="entry name" value="PTHR30510"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04285">
        <property type="entry name" value="DUF444"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02877">
        <property type="entry name" value="spore_yhbH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="43301" checksum="E226EE90F0832D45" modified="2004-10-11" version="1">MASTSRHDWSLHRKGPVDQARHNEKVKEAIRQNLPEIIAEESIITSDGKRLVKVPIRTLEEYRFRFNPNQGKRVGQGDGDSKVGDVIARARPGGQGGGGTQPGDQPGVDYYEAEVTVDDLAELIFEDLGLPDLRPKSTQEIPTEEVRFTEVRKKGPMSNLDRRRTIMENLKRNARRGMPVLGNITNDDLRFKVWEQEMRPSSSAVVIAMRDASGSMGEFKKYITRSLFFWMIRFLRTKYQDVEIVFITHHSEAQEVDEEAFFKMGESGGTRVSSAYKLALEIIQERYDPERWNIYPFHFSDGDNWSDNDNRLCVELVGKLLEVCNLFGYGEIREGGYTSSLMSAFSAIKDPRFVIVTITEKRDVYPALQKWFSRGGMAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL3</accession>
    <name>Q67RL3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH695</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH695"/>
    <dbReference type="EnsemblBacteria" id="BAD39680">
        <property type="protein sequence ID" value="BAD39680"/>
        <property type="gene ID" value="STH695"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH695"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="RARCEEP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="282" mass="30023" checksum="B5A2AF4DEEF93EC7" modified="2004-10-11" version="1">MISLTQVTMRYGGTVALDGVDLQVAAGETLGLLGPNGAGKTTALRLLAGMAPPFRGRVSVMGVDPWSEPDRVRTRMGVLPEGTGLFDRLTVGENLALFAGLYGLGRESVQRALDATGAAHLTGRRVGSLSKGERQRVALARAVLHDPELLILDEPTSGLDPAAAASLHQIIRDMQARGRTVVLASHDMAEVEALCDRVAILDRGRLVACDAPAALKARHGRRWVRVTVAGSEGPETLRWPLEEAAWVEVAARHQALGTLLAVHTEEATLSEVFLKTTGRELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP3</accession>
    <name>Q67RP3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH665"/>
    <dbReference type="EnsemblBacteria" id="BAD39650">
        <property type="protein sequence ID" value="BAD39650"/>
        <property type="gene ID" value="STH665"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH665"/>
    <dbReference type="eggNOG" id="COG1203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034285_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013381">
        <property type="entry name" value="CRISPR-assoc_prot_Cse1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09481">
        <property type="entry name" value="CRISPR_Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02547">
        <property type="entry name" value="casA_cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="523" mass="58667" checksum="5E2140921A8B5540" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP5</accession>
    <name>Q67RP5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas1</name>
        <name evidence="9" type="ordered locus">STH663</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH663"/>
    <dbReference type="EnsemblBacteria" id="BAD39648">
        <property type="protein sequence ID" value="BAD39648"/>
        <property type="gene ID" value="STH663"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH663"/>
    <dbReference type="eggNOG" id="COG1518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077904_0_0_9"/>
    <dbReference type="OMA" id="IWVGEAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09719">
        <property type="entry name" value="Cas1_I-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033641">
        <property type="entry name" value="Cas1_I-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019851">
        <property type="entry name" value="CRISPR-assoc_Cas1_ECOLI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03638">
        <property type="entry name" value="cas1_ECOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="307" mass="34401" checksum="282D7142D90CE1FD" modified="2004-10-11" version="1">MRNLRILPKVRDSWSYLYVEHARIDQEDKGICIHDAKGRVHVPCASLSLLMLGPGTSVTHAAVMAMVEAGCLVAWCGEQGVRFYAQGMGETRSAVNLMQQARFWADPQRRMQVVRRMYEMRFGEPLDPSLSLRQIRGKEGARVRTAYARASETYGVPWSGRNYDRNDWDRSDPVNRALSAANACLYGVCHAAIVAAGYSPALGFIHTGKMLSFVYDVADLYKTEITIPAAFMEVAKGVQRLESRVRHRCRDLFAERRLLERIVTDLARLFDLDPNPEPEVDLEVALPGDLWDPEGPVAGGRNFGGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU6</accession>
    <name>Q67RU6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Corrinoid adenosyltransferase</fullName>
            <ecNumber evidence="4">2.5.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cob(II)alamin adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Cob(II)yrinic acid a,c-diamide adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Cobinamide/cobalamin adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH612</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:28671"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16304"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:11528"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:58503"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Cob(I)alamin adenosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH612"/>
    <dbReference type="EnsemblBacteria" id="BAD39597">
        <property type="protein sequence ID" value="BAD39597"/>
        <property type="gene ID" value="STH612"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH612"/>
    <dbReference type="eggNOG" id="COG2096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_2_9"/>
    <dbReference type="OMA" id="VSYCHIA"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:cob(I)yrinic acid a,c-diamide adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="PTHR12213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="SSF89028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cob_adeno_trans" evidence="5">
        <location>
            <begin position="6"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="20791" checksum="CC1E47289538AC9F" modified="2004-10-11" version="1">MRPMRIYTRTGDKGDTSLRWGERVPKNALRVEVYGTVDEANAHVGMAVAMMEGPAFAAVRDVLLRVQNELFYVGADLATPPNRDRGEQPRITQASVDALEADIDRLEAGLPPLRNFVLPGGSAPAAALHVARTVVRRAERLLVSLMQQEPVDPALLRYLNRLSDLLFVAARAANQAAGHGDVLVDWNRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S14</accession>
    <name>Q67S14_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ferritin</fullName>
            <ecNumber evidence="5">1.16.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Iron-storage protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>4 Fe(2+) + 6 H2O + O2 = 12 H(+) + 4 iron(III) oxide-hydroxide</text>
            <dbReference type="Rhea" id="RHEA:11972"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:78619"/>
            <dbReference type="EC" id="1.16.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the ferritin family. Prokaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.2" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH544"/>
    <dbReference type="EnsemblBacteria" id="BAD39529">
        <property type="protein sequence ID" value="BAD39529"/>
        <property type="gene ID" value="STH544"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH544"/>
    <dbReference type="eggNOG" id="COG1528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065681_1_2_9"/>
    <dbReference type="OMA" id="CEDKGFE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="PTHR11431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="6">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="17972" checksum="D913252FDCF9497A" modified="2004-10-11" version="1">MNKAMEAALNEQIKLELHSAYTYLGMAAYCESTNLPGMAHWLELQAKEELEHAMKIYGHVNDRGGRVALKAIPEPVLDYASPLAVFEAVLAHEQKVTASIHKLYALAVEEKDYASLPLLQWFIEEQVEEESSADEVLQKIRLAGDSKSALLFLDSQLGSRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S76</accession>
    <name>LEPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 4</fullName>
            <shortName evidence="1">EF-4</shortName>
            <ecNumber evidence="1">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lepA</name>
        <name type="ordered locus">STH482</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194616.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S76"/>
    <dbReference type="SMR" id="Q67S76"/>
    <dbReference type="STRING" id="292459.STH482"/>
    <dbReference type="EnsemblBacteria" id="BAD39467">
        <property type="protein sequence ID" value="BAD39467"/>
        <property type="gene ID" value="STH482"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH482"/>
    <dbReference type="eggNOG" id="COG0481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_9"/>
    <dbReference type="OMA" id="MVQIAIQ"/>
    <dbReference type="OrthoDB" id="182107at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000176358" description="Elongation factor 4">
        <location>
            <begin position="1"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <sequence length="603" mass="67825" checksum="83464ECB8CB8A177" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF4</accession>
    <name>Q67SF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative NADH-ubiquinone oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194538.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH404"/>
    <dbReference type="EnsemblBacteria" id="BAD39389">
        <property type="protein sequence ID" value="BAD39389"/>
        <property type="gene ID" value="STH404"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH404"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_5_9"/>
    <dbReference type="OMA" id="FFNRFAA"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="8"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="33060" checksum="D749ACE632562120" modified="2004-10-11" version="1">MAVVLVAGGTGFIGSYIVRRLTQDGHRVIVMSRDPGKARGRVPDGVEVRAGDVTDGATLGPALAGAEIVVCAVQFPNHPVENPRRGHTYIRVDGEGTVRLVGAARKAGVSRFVYISGAGTREGQTKPWFRAKLMAEKAIRESGIPYTIFRPSWVYGPEDRSLNKFATFARLLPFVPVIGSGRTRVQPLYVEDLADAVAASLRTGAALNRTYDIGGPQELTMDEIIRTMLWVMGRRRPLLHSPAWLMKAAAWPLQFLPTPPLSPGAVDFVLMEEPVDNGQVLQDLGLTLTPLERGLSYLRPEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK9</accession>
    <name>Q67SK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PTS system enzyme IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH349</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH349"/>
    <dbReference type="EnsemblBacteria" id="BAD39334">
        <property type="protein sequence ID" value="BAD39334"/>
        <property type="gene ID" value="STH349"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH349"/>
    <dbReference type="eggNOG" id="COG1264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012312_8_4_9"/>
    <dbReference type="OMA" id="MGKAHNI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00212">
        <property type="entry name" value="PTS_IIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036878">
        <property type="entry name" value="Glu_permease_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018113">
        <property type="entry name" value="PTrfase_EIIB_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001996">
        <property type="entry name" value="PTS_IIB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00367">
        <property type="entry name" value="PTS_EIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55604">
        <property type="entry name" value="SSF55604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00826">
        <property type="entry name" value="EIIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51098">
        <property type="entry name" value="PTS_EIIB_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01035">
        <property type="entry name" value="PTS_EIIB_TYPE_1_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIB type-1" evidence="5">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate; for EIIB activity" evidence="4">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="79" mass="8549" checksum="40FFB2F733905BB5" modified="2004-10-11" version="1">MDDRTRAQEILAALGGKENVTELESCITRLRLVVNNPGAVDEKRLKAAGAVGVMKVGQVVQVILGTSAERIEQIMKDMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST7</accession>
    <name>Q67ST7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH271"/>
    <dbReference type="EnsemblBacteria" id="BAD39256">
        <property type="protein sequence ID" value="BAD39256"/>
        <property type="gene ID" value="STH271"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH271"/>
    <dbReference type="HOGENOM" id="CLU_2439679_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="90" mass="10400" checksum="BF2969518F9D33BE" modified="2004-10-11" version="1">MWNRCLELHRQGRQQGRWLDEGELKALTKGGQFALHAASVQAVVELFVECLERTRAMRRAGRCVFRTDSTTHSGRIRPLITDEIDHRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST8</accession>
    <name>Q67ST8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39255">
        <property type="protein sequence ID" value="BAD39255"/>
        <property type="gene ID" value="STH270"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH270"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041900_10_1_9"/>
    <dbReference type="OMA" id="RYNDYII"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002560">
        <property type="entry name" value="Transposase_DDE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01610">
        <property type="entry name" value="DDE_Tnp_ISL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_ISL3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="226" mass="26690" checksum="6ED690A33D0FDDCA" modified="2004-10-11" version="1">MYNTVFVDLEERRILDVVETREKKPVREHLKQYEKSVQAVCIDLNEAFRQAVRQALPEAEIVADKFHVIRLANYALNAVRKRVRRQIKGDRTHPIFRSRSVLLRNFEDLSSSQRKRLSQLLALSPELRAAYAAKERLRRWYNTSTTENSSHRLAQLISWLLASGVPELKHLATTFLRWQRELANYHRYRISNSITEGLNNKIKVIKRQAYGFRSFENFRRKILLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV2</accession>
    <name>Q67SV2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH256"/>
    <dbReference type="EnsemblBacteria" id="BAD39241">
        <property type="protein sequence ID" value="BAD39241"/>
        <property type="gene ID" value="STH256"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH256"/>
    <dbReference type="eggNOG" id="COG3209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1142135_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="24629" checksum="23FD856934C11363" modified="2004-10-11" version="1">MPASRREAGEGAGVGCSGSKGVPPPHPHPLAIGRRCHGGWPYMPASRREAGEGAGVGCSGSKGVPPPHPHPLAIDRRCHGGWPYMPASRREAGEGAGVGCSGSKGVPPPHPHPLAIGRRCHGGWPYMPASRREAGEGAGVGCSGSKGVPPPHPHPLAIGRRCHGGWPYMPASRREAGEGAGVGCSGSKGVPPPHPHPLAIDRRCHGGWPYMPASRREAGEGAGVGCSGSKGVPPPAGNRQAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ3</accession>
    <name>Q67SZ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH215"/>
    <dbReference type="EnsemblBacteria" id="BAD39200">
        <property type="protein sequence ID" value="BAD39200"/>
        <property type="gene ID" value="STH215"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH215"/>
    <dbReference type="eggNOG" id="COG1392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086031_1_0_9"/>
    <dbReference type="OMA" id="YWLLNEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="23022" checksum="42BF35F7606E8D63" modified="2004-10-11" version="1">MIFISNRSQQFYQLLREATENAAEVAGLLGRLLEEPVHVGNLAPEIKQLESKGDDLTHRLFELIHKTFATPLERTDLATLGVAIDNVVDAMEAAAARVGIYRLSASDKHLRAFAQILRAQATELVAAIDLLAGGKLLEIQDRIRQINTLENHGDDELRLALSELYDRSASDPVLFITMKEIYEMLEQATDEVETVANVLEGVIMKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T21</accession>
    <name>Q67T21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative gluconeogenesis factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH187</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for morphogenesis under gluconeogenic growth conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gluconeogenesis factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH187"/>
    <dbReference type="EnsemblBacteria" id="BAD39172">
        <property type="protein sequence ID" value="BAD39172"/>
        <property type="gene ID" value="STH187"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH187"/>
    <dbReference type="eggNOG" id="COG0391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044041_0_0_9"/>
    <dbReference type="OMA" id="LCGDDDW"/>
    <dbReference type="OrthoDB" id="665169at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00973">
        <property type="entry name" value="Gluconeogen_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010119">
        <property type="entry name" value="Gluconeogen_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30135">
        <property type="entry name" value="PTHR30135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01826">
        <property type="entry name" value="CofD_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="56"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="457" mass="49571" checksum="A279822FDBB1B45A" modified="2004-10-11" version="1">MRILRWFAPGLHIKRWLLVAVLGVALVVVGLSLTLRINLYRLLGAWLVENVAASRAVFYTGPVVAGVAALALGLVLTWVGISRLVNSVLSTLTAREEDVADAFLERRLLVRGPRVVAVGGGTGLPATLRGMKNYTANISAVVTVADDGGSSGRLRTEFGILPPGDIRNCLIALADIEPLMERLFQYRFTNGEGLAGHPFGNLFILAMSETTGDFYQAVKAASEVLAVRGRVLPSTLDHVVLRAELADGRMVSGESAIGRAGSPIRRVFLDPADPGGKIAALDDVLSAIAEAELIVLGPGSLYTSIMPNLLVPGVADAIRRSPALKIYVCNIMTEPGETDGFKVSDHMKALIDHGGFGLFDVCLVNTRQVPARLRERYREEGAEPVVLDPAAQRMGVQVVARDLLRIEGDYVRHDPEKLAQAIGHVFLARRPHVGRTPWEFFLFRQRLRERSRQEGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T23</accession>
    <name>Q67T23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histidinol phosphatase and related hydrolases of PHP family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194320.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH185"/>
    <dbReference type="EnsemblBacteria" id="BAD39170">
        <property type="protein sequence ID" value="BAD39170"/>
        <property type="gene ID" value="STH185"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH185"/>
    <dbReference type="eggNOG" id="COG1387">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1011661_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="29290" checksum="538594464461FDA4" modified="2004-10-11" version="1">MRIIADLHVQVRRGPAEGPVAAQVSAALVRGLEAVALFAPHSPAALPVLRAVRAIDARTPTLRVLSGVSCRILSPEGDLRLHTRAGREHDLVLAVAAPQVATALARWVPRYRPAARRALTDAVVAAVYRHDVDLVALPARPEVDPAEVARALRDRGVALEVCARRPRPPAALLRQLARLGVRFAVTSGAGRPEEVGDCGAAVALLAAAGVPPEQVVNSDRGGLTEWLAARRRLSDPGGWADWSRQGGTEGRERPGGRERRPGDWADWSAQGGEIH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-09-22" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T51</accession>
    <name>THIME_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Thiamine biosynthesis bifunctional protein ThiM/ThiE</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Hydroxyethylthiazole kinase</fullName>
                <ecNumber evidence="2">2.7.1.50</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>4-methyl-5-beta-hydroxyethylthiazole kinase</fullName>
                <shortName>TH kinase</shortName>
                <shortName>Thz kinase</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>Thiamine-phosphate synthase</fullName>
                <shortName>TMP-PPase</shortName>
                <shortName>TP synthase</shortName>
                <shortName>TPS</shortName>
                <ecNumber evidence="3">2.5.1.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Thiamine-phosphate pyrophosphorylase</fullName>
                <shortName>TMP pyrophosphorylase</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="primary">thiM/thiE</name>
        <name type="ordered locus">STH157</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the Thz kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.50" evidence="2"/>
    <dbReference type="EC" id="2.5.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T51"/>
    <dbReference type="SMR" id="Q67T51"/>
    <dbReference type="STRING" id="292459.STH157"/>
    <dbReference type="PRIDE" id="Q67T51"/>
    <dbReference type="EnsemblBacteria" id="BAD39142">
        <property type="protein sequence ID" value="BAD39142"/>
        <property type="gene ID" value="STH157"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH157"/>
    <dbReference type="eggNOG" id="COG0352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568500_0_0_9"/>
    <dbReference type="OMA" id="RRRMFLN"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00139"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004417">
        <property type="term" value="F:hydroxyethylthiazole kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000383912" description="Thiamine biosynthesis bifunctional protein ThiM/ThiE">
        <location>
            <begin position="1"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Hydroxyethylthiazole kinase">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine-phosphate synthase">
        <location>
            <begin position="288"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>5-(2-hydroxyethyl)-4-methylthiazole</name>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>5-(2-hydroxyethyl)-4-methylthiazole</name>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="303"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="400"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="451"/>
            <end position="452"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P39593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="P39594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="480" mass="48683" checksum="0FEC441B02894F29" modified="2004-10-11" version="1">MSTLPERVRERRPLVHAITNCVTMEWVARGLLAAGARPVMARDAAEAPVVAAAADALVLNLGTWSPGLQQAMLEAGQVAARRGIPVVLDPVGAGGTETRTRAALELLERVRVTAVRGNAGEILALAGRDGLVRGVDGPDGRPGPQTERAARAVARRFGCLVAVTGATDLVTDGRRTLAVRAGHPLMSQVPGTGCLATALVAAALAAGTGAGPAGRDRPMEDVDVVAEALLWAGWAGEQAASAASGPGTFAAAFLDRLALRGPLPPGRIAPPLSERLSLYVLVSGATPPDVLEAVLQAGCRMIQFREKRLPLPAQLEAAARVREACRRHGALLVVNDRVDLALAVGADGVHLGQEDLPVAAARRILGPDAVIGATCETAGEARAARDAGADYIGAGPVYVTPSKPDAGEPYGPDVVRRVSEAADLPVVGIGGIGPGRAAPVIAAGAAGVAVISAVLGAPDPGAAARAILDEVRRAKGEVSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T57</accession>
    <name>UVRB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein B</fullName>
            <shortName evidence="1">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrB</name>
        <name type="ordered locus">STH151</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194286.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T57"/>
    <dbReference type="SMR" id="Q67T57"/>
    <dbReference type="STRING" id="292459.STH151"/>
    <dbReference type="PRIDE" id="Q67T57"/>
    <dbReference type="EnsemblBacteria" id="BAD39136">
        <property type="protein sequence ID" value="BAD39136"/>
        <property type="gene ID" value="STH151"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH151"/>
    <dbReference type="eggNOG" id="COG0556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009621_2_1_9"/>
    <dbReference type="OMA" id="EYVDRMV"/>
    <dbReference type="OrthoDB" id="95696at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000227373" description="UvrABC system protein B">
        <location>
            <begin position="1"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="25"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="1">
        <location>
            <begin position="431"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="620"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <sequence length="659" mass="75407" checksum="CD202F659C8EB5B3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T85</accession>
    <name>Q67T85_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome hibernation promoting factor</fullName>
            <shortName evidence="2">HPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpf</name>
        <name evidence="4" type="ordered locus">STH123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with 100S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194258.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH123"/>
    <dbReference type="EnsemblBacteria" id="BAD39108">
        <property type="protein sequence ID" value="BAD39108"/>
        <property type="gene ID" value="STH123"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH123"/>
    <dbReference type="eggNOG" id="COG1544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071472_0_3_9"/>
    <dbReference type="OMA" id="HGGYGVI"/>
    <dbReference type="OrthoDB" id="1766263at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00839">
        <property type="entry name" value="HPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034694">
        <property type="entry name" value="HPF_long/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="3">
        <location>
            <begin position="119"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="19965" checksum="C1EF0AD34BE130DD" modified="2004-10-11" version="1">MQVAVRGKNIEITKALREYVEKKLGKIEKFVDHPIKAQANLYVERGRHIIEVTADLNGWILRGEEATGDMYASIDLVADKLEKQVQRYRARLRRRGGAGASPEAAVAGAEDQGVDAELDGKVVKVKRFPVKPVTVDEAILQMDLLSHDFFVFVNSDTGKVNVLYRRHDGDYGLLVPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA8</accession>
    <name>Q67TA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194235.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH100"/>
    <dbReference type="EnsemblBacteria" id="BAD39085">
        <property type="protein sequence ID" value="BAD39085"/>
        <property type="gene ID" value="STH100"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH100"/>
    <dbReference type="HOGENOM" id="CLU_1098063_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014794">
        <property type="entry name" value="DUF1779"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036209">
        <property type="entry name" value="YwmB-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08680">
        <property type="entry name" value="DUF1779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143842">
        <property type="entry name" value="SSF143842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27419" checksum="3557F08027A2BA30" modified="2004-10-11" version="1">MRPRNAHPRPVRRAGPRLAPEQTLTRQQVSLPLLLSLALALALVAAPLWAFPGLRPAAERPRRESATPLPGPALEMLNRAWEAAGDRLEQALLFLQEPVEDPQVAERLAAGLGWGADAPTGEERSLRLVDGIGGPYVEVTWRLRGEAAARWDERYRELRQVLAAEGLWPQVQVELSGRAAGGQPLALAGAALDALGARQREPWSDGRAASVAGYTPLLPPGPYAVNVQAAVRRDGDGDGNRLWIGWPTVRSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC7</accession>
    <name>Q67TC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Undecaprenyl-phosphate N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH81</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194216.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH81"/>
    <dbReference type="EnsemblBacteria" id="BAD39066">
        <property type="protein sequence ID" value="BAD39066"/>
        <property type="gene ID" value="STH81"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH81"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_2_4_9"/>
    <dbReference type="OMA" id="MCLGFLP"/>
    <dbReference type="OrthoDB" id="728505at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="358" mass="37786" checksum="C212E19F5F3CA6AC" modified="2004-10-11" version="1">MLQQAGLTFAAALVASLLLTPLARRLALRTGTVAPVVKRSVHTEPKPFLGGLAMYLAFALAMILAGGLRDPQIVGILVCGGLLVLLGIRDDRSPMRPRTKFLGQVAVAALMVYVPYWQLQVNAIYSPLGDRWVSFGPLAGPLTILWIVSFINVMNFIDGLDGLAAGISTIAALTLLVVSLQQGMPSAILLSAALAGSAVGFLRYNFNPAKIFMGDAGSMFLGFTLAAISVDGVMKSALAVSVLVPVLALAVPIFDTAFAIIRRVAAGLPIGQADKDHLHHRLLRLGLSHRKTVLVMWAVSAALGLSAVALTEVNFAEAMVIVAVVLTIVVVAARRLGLLEVTETRHSIRAADSGEDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J40</accession>
    <name>Q67J40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3329"/>
    <dbReference type="EnsemblBacteria" id="BAD42310">
        <property type="protein sequence ID" value="BAD42310"/>
        <property type="gene ID" value="STH3329"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3329"/>
    <dbReference type="HOGENOM" id="CLU_1601851_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="166" mass="18410" checksum="005286FDBF9AD45F" modified="2004-10-11" version="1">MDTEPFCSYRTGLLRQAPSPGAPVGDVPRGTTRKVVGPQAEKYTRPAVMQGTRTGSDRTPSGLMRRFSTDVPRGTLPPIERADMPPQHRPALLVKRRQVARVTRHMVLTVRHDTPRGRRFTGMGDCPDLADNRRSVTGRTGTLPARGLAKALTTCRLRMARRKMTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J78</accession>
    <name>Q67J78_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PF03932 family protein CutC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cutC</name>
        <name evidence="3" type="ordered locus">STH3291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CutC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Once thought to be involved in copper homeostasis, experiments in E.coli have shown this is not the case.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197403.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3291"/>
    <dbReference type="EnsemblBacteria" id="BAD42272">
        <property type="protein sequence ID" value="BAD42272"/>
        <property type="gene ID" value="STH3291"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3291"/>
    <dbReference type="eggNOG" id="COG3142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050555_3_1_9"/>
    <dbReference type="OMA" id="CRWTFHR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00795">
        <property type="entry name" value="CutC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005627">
        <property type="entry name" value="Cu_homeostasis_CutC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036822">
        <property type="entry name" value="CutC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12598">
        <property type="entry name" value="PTHR12598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03932">
        <property type="entry name" value="CutC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110395">
        <property type="entry name" value="SSF110395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="23646" checksum="27CEFEE728983671" modified="2004-10-11" version="1">MKRVTLEVIAVTVADAVAAEAGGADRIELVASMAEGGVTPSAGVIAAVRRATRLPVYVMIRPRGGSFLFSPEEVEAMVTDARIARDLGADGLVVGALTPEGDVDRTALERILTEAGLPATFHRAFEEIIHREGALAQVASLPHVERILTGGGAARPEEALDTLRELVQRSPLEIQIGGGVTPANAARLVRETGASALHVGSAVRDSAGGVSAARVAELRRIIDGATAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF0</accession>
    <name>Q67JF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3218</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197331.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3218"/>
    <dbReference type="EnsemblBacteria" id="BAD42200">
        <property type="protein sequence ID" value="BAD42200"/>
        <property type="gene ID" value="STH3218"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3218"/>
    <dbReference type="eggNOG" id="COG1178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_1_1_9"/>
    <dbReference type="OMA" id="VGQQQFG"/>
    <dbReference type="OrthoDB" id="1783638at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="519"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="69"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="350"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="556" mass="60461" checksum="6DC3869E2012C439" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG0</accession>
    <name>Q67JG0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Iron hydrogenase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH3208</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3208"/>
    <dbReference type="EnsemblBacteria" id="BAD42190">
        <property type="protein sequence ID" value="BAD42190"/>
        <property type="gene ID" value="STH3208"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3208"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_2_9"/>
    <dbReference type="OMA" id="CDDVIMD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="560"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="12">
        <location>
            <begin position="592"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD42190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="618" mass="66765" checksum="D6C3D40F2AC55F41" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI1</accession>
    <name>Q67JI1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">7,8-dihydroneopterin aldolase</fullName>
            <ecNumber evidence="3">4.1.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH3187</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHNA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197300.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3187"/>
    <dbReference type="EnsemblBacteria" id="BAD42169">
        <property type="protein sequence ID" value="BAD42169"/>
        <property type="gene ID" value="STH3187"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3187"/>
    <dbReference type="eggNOG" id="COG1539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112632_1_3_9"/>
    <dbReference type="OMA" id="GHYKSVA"/>
    <dbReference type="OrthoDB" id="1858654at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00154"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00534">
        <property type="entry name" value="DHNA_DHNTPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="PTHR42844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00525">
        <property type="entry name" value="folB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FolB" evidence="4">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD42169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="13621" checksum="08238B08F21895F8" modified="2004-10-11" version="1">MDRIVISGMRFYAYHGVLPEETRLGQEFLVDAELYLDLQAAGQEDDIRQTVHYGKVYQTVKAIVEGTPFKLIEAVAERVAAEVLAQYRNVEAVTIRVHKPRAPIPGALGGVMVEIQRRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI6</accession>
    <name>Q67JI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3182"/>
    <dbReference type="EnsemblBacteria" id="BAD42164">
        <property type="protein sequence ID" value="BAD42164"/>
        <property type="gene ID" value="STH3182"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3182"/>
    <dbReference type="HOGENOM" id="CLU_395292_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269229" evidence="1">
        <location>
            <begin position="24"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="697" mass="75250" checksum="027AF891E210FB56" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK2</accession>
    <name>Q67JK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltranspeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197279.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3166"/>
    <dbReference type="MEROPS" id="T03.025"/>
    <dbReference type="EnsemblBacteria" id="BAD42148">
        <property type="protein sequence ID" value="BAD42148"/>
        <property type="gene ID" value="STH3166"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3166"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_1_9"/>
    <dbReference type="OMA" id="MFCLFYD"/>
    <dbReference type="OrthoDB" id="803717at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="535" mass="58103" checksum="9EA6C91F1532E708" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN8</accession>
    <name>Q67JN8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3343 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3130"/>
    <dbReference type="EnsemblBacteria" id="BAD42112">
        <property type="protein sequence ID" value="BAD42112"/>
        <property type="gene ID" value="STH3130"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3130"/>
    <dbReference type="HOGENOM" id="CLU_167443_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021778">
        <property type="entry name" value="Se/S_carrier-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11823">
        <property type="entry name" value="DUF3343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3343" evidence="1">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="79" mass="8654" checksum="8A9EA0E371C6DA62" modified="2004-10-11" version="1">MPRGRDGMDYGVALFHTTSMALKAEQRLKAAGLAVKLIPTPRQFSSDCGFALRFAWADCARVEELLRTGGVETAGLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ4</accession>
    <name>Q67JZ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH3023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197138.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3023"/>
    <dbReference type="EnsemblBacteria" id="BAD42006">
        <property type="protein sequence ID" value="BAD42006"/>
        <property type="gene ID" value="STH3023"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3023"/>
    <dbReference type="eggNOG" id="COG1101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="VMLHQGN"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD42006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="267" mass="29069" checksum="E05261C6C7DA6A45" modified="2004-10-11" version="1">MTGLTIEGVHKTFFPGTVNEIRALKGVDLTLAPGEFVTVVGSNGAGKSTLLNSVAGVIAPDRGRILLGDVDITRLTEVQRAARIGRVLQNPLAGTAPSLTVAENLALALARGRSRGLRWALTAARRRMFAEELAQLGLGLENRLDDKVGLLSGGQRQALSLLMATLQRPDVLLLDEHTAALDPRAAALIAELTNRWVRQHRLTTLMITHNLEQAIRLGDRLVMMHEGRILFTLSGPEKQGLTIEGLRQAFERVRGQEFSYDRAVLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K04</accession>
    <name>Q67K04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Electron transfer flavoprotein alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197128.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3013"/>
    <dbReference type="EnsemblBacteria" id="BAD41996">
        <property type="protein sequence ID" value="BAD41996"/>
        <property type="gene ID" value="STH3013"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3013"/>
    <dbReference type="eggNOG" id="COG2025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_1_9"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="OrthoDB" id="1400253at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="PTHR43153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="5">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="33572" checksum="75913D42DA52828D" modified="2004-10-11" version="1">MARNILVVAEVRNGAIRPVSFECIAAGREIAEGGAITAVLLGQQGVDASELIRRGADRVVVVKGDHLANYTPDGWLSALQQVYLQEKPDVVLMGHTGIGRDLLPRFAMRNAAGMISDAIAVKVEGGQVVFTRPIYAGKAYATQVVKDGLIVATIRPNNVAALEADPGRTGDVSEAEAASVHLRTVVKEVVRKATTGVDLTAAKVVVAGGRGVRSAEGFKPLYELAEVLGGAVGASRAAADAGYCDYALQIGQTGKTVTPDLYIACGISGAIQHLAGMSASKVIVAINKDPDAPIFKVADYGIVGDLFEVVPLLTREFKALLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K27</accession>
    <name>Q67K27_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Flagellar biosynthetic protein FlhB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">flhB</name>
        <name evidence="14" type="ordered locus">STH2988</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the type III secretion exporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197103.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2988"/>
    <dbReference type="MEROPS" id="N06.A01"/>
    <dbReference type="EnsemblBacteria" id="BAD41971">
        <property type="protein sequence ID" value="BAD41971"/>
        <property type="gene ID" value="STH2988"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2988"/>
    <dbReference type="eggNOG" id="COG1377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_1_2_9"/>
    <dbReference type="OMA" id="DEYKQQE"/>
    <dbReference type="OrthoDB" id="1424100at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006136">
        <property type="entry name" value="FlhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00950">
        <property type="entry name" value="TYPE3IMSPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00328">
        <property type="entry name" value="flhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 12" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0969">Cilium</keyword>
    <keyword evidence="14" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD41971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="361" mass="39960" checksum="DA03B4C74D2B92D6" modified="2004-10-11" version="1">MDLQLFAERTEEPTPRRLQKAREEGRVARSTDLVAGLGLVAATLALRGLGGYSVQQVTGGMVGAFETLRPLELTPETTAAVMQDWALIGLRAVLPIALVVVAIGVVAGTLQTRFIFTLRALVPRFATLNPITGLTRIFSLRTVTELVKGLIKLAAIGYIAYRSVRGLVPQFPNLIAQTVPAGLSAIADMVLDVMLAVGLAYLVVGVLDYGYQYWEFRRSMRMTKQEVKQEHREQEGAPELKSRQRQRMRELAMRRRALKEVPGADVVVTNPTHFAVALKYEAAKDEAPRVVAKGADLLALEIQKIARAHDVPLVENRTLARGLYYDVEVGRLIPPEYYQAVAEVLAFVYNLRRQSRQAGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K30</accession>
    <name>Q67K30_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagellar biosynthesis switch protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">flhG</name>
        <name evidence="3" type="ordered locus">STH2985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197100.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2985"/>
    <dbReference type="EnsemblBacteria" id="BAD41968">
        <property type="protein sequence ID" value="BAD41968"/>
        <property type="gene ID" value="STH2985"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2985"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_0_9"/>
    <dbReference type="OMA" id="FKVAKNN"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02038">
        <property type="entry name" value="FlhG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033875">
        <property type="entry name" value="FlhG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="31516" checksum="E768241EAEDCB05F" modified="2004-10-11" version="1">MFDQASRLRELAAAYRNQAAGRAPRRFEAIAVTSGKGGVGKTNLAVNLAQVLVKDGHEVLLMDVDLGLANANILLGTVPPYHLGHFLRGEVDILQVIHRTDTGLKLIAGGSGLVELGSLTAAQLRPILRSLERLEGEAEYLILDTGAGVGDAVLEFALAADQVLVVTTPEPTAMADAYTMIKALVARDPGARIRLVVNQAERPEDAQRAAERIVTTARNFLGVEVVHLGTVPRDPHVWQSVRRRVPYVLGYPASPAARAVEAMASRLVGAQVPAPSAPGSFFGRLASLFGRRSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K39</accession>
    <name>Q67K39_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flagellar secretion chaperone FliS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fliS</name>
        <name evidence="7" type="ordered locus">STH2976</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FliS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2976"/>
    <dbReference type="EnsemblBacteria" id="BAD41959">
        <property type="protein sequence ID" value="BAD41959"/>
        <property type="gene ID" value="STH2976"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2976"/>
    <dbReference type="eggNOG" id="COG1516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080373_1_2_9"/>
    <dbReference type="OMA" id="AKRHMEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16098">
        <property type="entry name" value="FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003713">
        <property type="entry name" value="FliS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036584">
        <property type="entry name" value="FliS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34773">
        <property type="entry name" value="PTHR34773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02561">
        <property type="entry name" value="FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039090">
        <property type="entry name" value="Flis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101116">
        <property type="entry name" value="SSF101116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00208">
        <property type="entry name" value="fliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="127" mass="14341" checksum="AAFEC043A5FDE6E8" modified="2004-10-11" version="1">MNQYKAYQVQTTAPEDQVALLYEGAQRFIDKAALALQQGRYGEVSAYVGRAQQIFTELRTALNPEAGEIARNLDRLYDYWTWRLSQGLLKKDIEAFREVSATVGDMAGAWREAARQVRAQRGTHVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K74</accession>
    <name>Q67K74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2941</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2941"/>
    <dbReference type="EnsemblBacteria" id="BAD41924">
        <property type="protein sequence ID" value="BAD41924"/>
        <property type="gene ID" value="STH2941"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2941"/>
    <dbReference type="HOGENOM" id="CLU_918062_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="33840" checksum="0362E8E4351117B3" modified="2004-10-11" version="1">MEQGGLSSVEQAGIHLIMSRCRLLLQDAPGAVLSGLSAVKLAEQEREYDLLGRALLALGAAHAAMQQFEQALSCFQRYFTYRAYYRDAARLEGAAWKSLGITYQRMLQPKQAVHALEAARTWFGGRQMEHGVFTTTHDLINVYLYLAKEDRSLRRPVRRLLIEQRGIAARRPTEPYYAGTYLLDLAAWLASGGYYGRAAAAAVRALFVHRDDPAHACHCYLLLARCELQLGEAHKALTFALAAQNEAVMAGRPELEQLAADALDEVVQREGVEILRHLDAAYQASARFPEEMPTVPLLRRDLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K77</accession>
    <name>ACPS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="1">Holo-ACP synthase</shortName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpS</name>
        <name type="ordered locus">STH2938</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197054.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67K77"/>
    <dbReference type="SMR" id="Q67K77"/>
    <dbReference type="STRING" id="292459.STH2938"/>
    <dbReference type="EnsemblBacteria" id="BAD41921">
        <property type="protein sequence ID" value="BAD41921"/>
        <property type="gene ID" value="STH2938"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2938"/>
    <dbReference type="eggNOG" id="COG0736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089696_3_1_9"/>
    <dbReference type="OMA" id="DERHYAV"/>
    <dbReference type="OrthoDB" id="1893660at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000175719" description="Holo-[acyl-carrier-protein] synthase">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <sequence length="125" mass="13676" checksum="BAA889E06B7E4DB8" modified="2004-10-11" version="1">MILGTGVDIVAVERVARAVERHGDRFLRRVFTPGELAYCTSSEAHRAARLAARFAAKEAVLKALGIGLREVRWTDAEVCRDERGRPSVRLTGRLAEIAAARGATRIHLSLSHTQEYAVAQVVIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K80</accession>
    <name>Q67K80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2935"/>
    <dbReference type="EnsemblBacteria" id="BAD41918">
        <property type="protein sequence ID" value="BAD41918"/>
        <property type="gene ID" value="STH2935"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2935"/>
    <dbReference type="eggNOG" id="COG0864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2720847_0_0_9"/>
    <dbReference type="OMA" id="ENECFAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="8401" checksum="B1BE70B66029FB87" modified="2004-10-11" version="1">MPASLLEQVDGLAALERTDRDALIQEAVRIYVEERKKRDMREQLRRGYREMSRINLTLAEEGPIFERIGSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K90</accession>
    <name>Q67K90_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycoprotein endopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197041.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2925"/>
    <dbReference type="EnsemblBacteria" id="BAD41908">
        <property type="protein sequence ID" value="BAD41908"/>
        <property type="gene ID" value="STH2925"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2925"/>
    <dbReference type="eggNOG" id="COG1214">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064886_0_0_9"/>
    <dbReference type="OMA" id="MEFCENI"/>
    <dbReference type="OrthoDB" id="1826274at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="1">
        <location>
            <begin position="32"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="24250" checksum="2D496BCB78C14434" modified="2004-10-11" version="1">MRVLAFDTATAACTVAVAADGQVLSEFTLQVPRAHSVRLMPLIAQTLREAGVDRRELDAIAVGVGPGSFTGLRIGLATAKGLALALDKPVVPVSTLAAAAYGTGAQAGLVVPLLDAKRDQVFTAVYAAGDRDPSTWTELLGPANLHIDEVVERVRELRARLDRPWPFITLCGDAAEQHAPRFGGDGAVRLAPAGSLLPRASSVAALGLSLLRSGGAMSPDALSVCPAWALTWR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA0</accession>
    <name>MURA2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2</fullName>
            <ecNumber evidence="1">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enoylpyruvate transferase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylglucosamine enolpyruvyl transferase 2</fullName>
            <shortName evidence="1">EPT 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murA2</name>
        <name type="ordered locus">STH2915</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197032.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KA0"/>
    <dbReference type="SMR" id="Q67KA0"/>
    <dbReference type="STRING" id="292459.STH2915"/>
    <dbReference type="EnsemblBacteria" id="BAD41898">
        <property type="protein sequence ID" value="BAD41898"/>
        <property type="gene ID" value="STH2915"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2915"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_9"/>
    <dbReference type="OMA" id="IETVWEN"/>
    <dbReference type="OrthoDB" id="537477at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231285" description="UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="23"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <sequence length="423" mass="45280" checksum="29B8250BB5A9BB3E" modified="2004-10-11" version="1">MTAKFVVRGGNRLSGTVTIGGAKNSALPIVTAAALAAEGESILENVPDNSDIQHLCEILRALGCRVERVAETTLRIQARELEHHVAPYHLARRLRGSTYVMGLLLARLGKGEVACPGGCEIGARPVDFHLKGFRALGAEVVVEHGAMVSRRVDLRGGRFYVDRASVGTTVNMIITASLAPGVTVLENAACEPEIVDLANFINAMGGRVRGAGTNTVRIEGVDRLRGARHEIIPDRIEAGTYMMMTAAAGGDVVVENVIPEHLGTVIAKLVEAGQEVEEHGDCIRVRARPIRAVDVETQVYPGFPTDLQSPWVALMGLADGISVVHETIFENRFGFTNELIRMGAKIKVDRNTGIIRGVKRYTGAPVEARDIRGGAALVTAALAAEGVTEVSGVQYIDRGYTRMEEKLAALGADIRRVPNGSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE9</accession>
    <name>Q67KE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196983.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2864"/>
    <dbReference type="EnsemblBacteria" id="BAD41849">
        <property type="protein sequence ID" value="BAD41849"/>
        <property type="gene ID" value="STH2864"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2864"/>
    <dbReference type="eggNOG" id="COG3238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068878_3_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006750">
        <property type="entry name" value="YdcZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34821">
        <property type="entry name" value="PTHR34821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04657">
        <property type="entry name" value="DMT_YdcZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="150" mass="15075" checksum="561C02B9FC13422E" modified="2004-10-11" version="1">MASWLFWLIIILGGVAGAVQAPVNATLAQHIRPIPASLVSFIVGGTALLALTLFTLRGQGLSGLGQGLSTAPPWSFLGGLCGAMVVSSVIVGTGAIGANATLSLLTGVQLIAALVIDAIGFGTAGPIPIRWPQVLGVLLIIGGMKLVLTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ3</accession>
    <name>GATB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">STH2820</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196939.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KJ3"/>
    <dbReference type="SMR" id="Q67KJ3"/>
    <dbReference type="STRING" id="292459.STH2820"/>
    <dbReference type="EnsemblBacteria" id="BAD41805">
        <property type="protein sequence ID" value="BAD41805"/>
        <property type="gene ID" value="STH2820"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2820"/>
    <dbReference type="eggNOG" id="COG0064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_9"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="OrthoDB" id="497127at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000241284" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="490" mass="54795" checksum="B65A8018CB5ED4C9" modified="2004-10-11" version="1">MSTAKWETVIGLEVHVELSTETKIWCGCKNEFGAEPNTNVCPVCLALPGALPVLNYKAVEYTIRAGLALNCKIQRHSKFDRKNYFYADLPSGYQISQFDLPLCYDGYVDITKKDGTTRRIRIKRIHLETDAGKLLHAGDDVAAADYSLVDFNRAGVPLIEIVTEPDLRSAEEAGLFLQKLRTILKYSGVSDVKMEEGSMRCDVNLSVRPAGSSEYGVRTELKNVNSFSAVMRGIEYEEKRHIRILEEGGQPEQETRSWRDAQGISVLLRSKEDAEDYRYFPEPDLPPLEVSAEEIERIRAGLPELPDALMQRLMTEYGLSAYDASVIVAEREYAQWFLHAVELAGAGQAKTVANWQINELYRVMNEKGLGPEQIPVTPEQLVGMLKLIEQGTITGKIAKTVFDKMVETGKDAETIVKEEGLTQVADEGELLAIAREVVASNPKVFEDWKAGKQSAAQWFVGQIMKRTRGRANPQMALKLVTQALEEQAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL0</accession>
    <name>Q67KL0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196922.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2803"/>
    <dbReference type="EnsemblBacteria" id="BAD41788">
        <property type="protein sequence ID" value="BAD41788"/>
        <property type="gene ID" value="STH2803"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2803"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2071967_0_0_9"/>
    <dbReference type="OrthoDB" id="1948031at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="118" mass="12660" checksum="6D79CE10D66F9B5B" modified="2004-10-11" version="1">MSERAAIATSCYDHLGGPLGERLARRLMELGWVTAEPAPGVTPEGWGGFARLGLDLSPLPAGRRKPVALCGKHIGAHLGFLLRRHLLEAGWIERTPDGFALTPAGEEALRQLGVDLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN3</accession>
    <name>Q67KN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41765">
        <property type="protein sequence ID" value="BAD41765"/>
        <property type="gene ID" value="STH2780"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2780"/>
    <dbReference type="HOGENOM" id="CLU_2940150_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="60" mass="6556" checksum="2A00D2B230BA2E65" modified="2004-10-11" version="1">MASMLAMMNRLMRIRRIRFSPFRCLSMKQPVGLPVRCTGSLFASARTSKAVSGAGLAPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU2</accession>
    <name>Q67KU2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2266</name>
        <name evidence="3" type="ordered locus">STH2719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">IAM14863</strain>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196390.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2266"/>
    <dbReference type="EnsemblBacteria" id="BAD41251">
        <property type="protein sequence ID" value="BAD41251"/>
        <property type="gene ID" value="STH2266"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41704">
        <property type="protein sequence ID" value="BAD41704"/>
        <property type="gene ID" value="STH2719"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2266"/>
    <dbReference type="KEGG" id="sth:STH2719"/>
    <dbReference type="eggNOG" id="COG3267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024125_1_0_9"/>
    <dbReference type="OMA" id="IICDFLM"/>
    <dbReference type="OrthoDB" id="947558at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="278" mass="31827" checksum="E9DE0FD09FD8DDB7" modified="2004-10-11" version="1">MLAEFFGFRVLPFSKEIPTTDLFPCAQHQELLARLQYCVRHRAFGLITGEVGSGKTTAIRTLYDLLDRSRNPFVYIADSKLTPTAFYRDVLTQLGVAAPYHFLSREARRLFEQTILDGYRLHGLQPVIVLDEAHLLPHAMLQEVRFLLNFHLDSACPMTFLLVGQSELRGMLRLRTFEAIAQRVQVRYHLGPLSEEESVAYIRHHLRRTGVDHDIFSEQALQKIAAESRGLPRVINTLCTNCLLDACSREQRFVDEANVDRVLFELQDLQTAKGSSPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L79</accession>
    <name>Q67L79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196704.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41567">
        <property type="protein sequence ID" value="BAD41567"/>
        <property type="gene ID" value="STH2582"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2582"/>
    <dbReference type="eggNOG" id="ENOG5032UTE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117091_0_0_9"/>
    <dbReference type="OrthoDB" id="1828245at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="204" mass="24290" checksum="68E586F82E46905B" modified="2004-10-11" version="1">MEPFAEHLYRRLAEGLLVSECPRLEEAYVLSLYVDFDDGPQRMKLWLYYNTPWQLRRAISQGEQPDEARWYFALWEPEFITAVGLSEQECDRMADTESHRLLARWLARRGLYRAPEELDALLADPGRYDAWESAARQSLLDLVIRVARRLHDTGIILCRFGRTIPLLVHQWEYDQWCLEATRKVNPPGLITEFERWWWLEWSCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L83</accession>
    <name>Q67L83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2578"/>
    <dbReference type="EnsemblBacteria" id="BAD41563">
        <property type="protein sequence ID" value="BAD41563"/>
        <property type="gene ID" value="STH2578"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2578"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_972968_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="286" mass="31374" checksum="48FDCB8048C0CED6" modified="2004-10-11" version="1">MPAESEKGVRTLPQDARILGTLSVQLAAEGRWQDALHTAHACLRLAEPGSVLYLWALHMLACTYVDAGLPQRARPYAQAYLRQAAGNPQLASYTPFVVRAMGHIAYQEHRFLSAFRWYKKAHALFCRQGDHVQAAVTSHNMAWALTRAGRPHRAREVLAPRHAFPAELAYLFDGAMAAILAAEGRLSDTIQRGHEALSAAGRRAHDLVDAAEVALFLARAYWRLREHGAASAWISRAAEFAALQGWRFVDVLHLNERAGGGEVPHAASPRGSANLHHRGCFTTGIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC2</accession>
    <name>PROA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="1">GPR</shortName>
            <ecNumber evidence="1">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proA</name>
        <name type="ordered locus">STH2539</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LC2"/>
    <dbReference type="SMR" id="Q67LC2"/>
    <dbReference type="STRING" id="292459.STH2539"/>
    <dbReference type="EnsemblBacteria" id="BAD41524">
        <property type="protein sequence ID" value="BAD41524"/>
        <property type="gene ID" value="STH2539"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2539"/>
    <dbReference type="eggNOG" id="COG0014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_0_9"/>
    <dbReference type="OMA" id="PGVCNAM"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000189797" description="Gamma-glutamyl phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <sequence length="425" mass="44252" checksum="9D6187BDEBC3032A" modified="2004-10-11" version="1">MDVRSMARQARAAQRQLELTSPEERNAALEAIARALEARAGQIVAANRADLAAAEEAGLPGPMIARLRLDEGKLAGVIRGVRAVAELPDPLAVEPQAWIRPNGLRIQRVRVPLGVVGIIYESRPGVTVDAAVLCLKAGNAVLLKGGKEAARSNAALGEAMAAGLQEVGLPVELAQVLPSTREAAQELMAARSLVDVLIPRGGAGLIRATVEQSQVPVIETGTGVCHVYVHRAADLAMAREIVLNAKCSNPAVCNAAETLLVDREVAAPFLAEAGPALLAAGVRLRACPEALAILQETARPDLAGAGPAAVEPATEEDWAAEYLDLCLAVKVVSGLDEALAHIARYGTGHSEAIVTGDAGAADRFLRSVDAAAVYHNASTRFTDGGEFGFGAEIGISTQKLHARGPMGLAELTTYKYVVLGNGQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH5</accession>
    <name>Q67LH5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenylacetic acid degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaA</name>
        <name evidence="1" type="ordered locus">STH2486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2486"/>
    <dbReference type="EnsemblBacteria" id="BAD41471">
        <property type="protein sequence ID" value="BAD41471"/>
        <property type="gene ID" value="STH2486"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2486"/>
    <dbReference type="eggNOG" id="COG3396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_879733_0_0_9"/>
    <dbReference type="OMA" id="MQPEANW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097266">
        <property type="term" value="F:phenylacetyl-CoA 1,2-epoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011881">
        <property type="entry name" value="PaaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02156">
        <property type="entry name" value="PA_CoA_Oxy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="35457" checksum="226DB5BAC58FABF7" modified="2004-10-11" version="1">MRTLDERHAEFLDRVTRGEKVEATDWMPDESRMALVKFLQMHALSELMGALPEKEWVPRAPTLARKLSLMAKVQDEVGHAQLILRVAEDLAAPLGKGREELVEELFTGECKFHNVFHMPAPTWADAGIIGWLVDGAAVVTQAALLTSSYAPYARVLQRVCAEEGFHIQHGESICLALAEGTPAQRRMLQEALERWWEPLLLFFGPPESNSPASAVLFRYRFRTKTNEELRQKFLSKYVPRIRALGLTIPDPELRYDEAAGRWIYTPPDWSKLKAIARNQGPLSQYRLGLRRLSYEQGRWVREALLTPAAQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI3</accession>
    <name>Q67LI3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2478"/>
    <dbReference type="EnsemblBacteria" id="BAD41463">
        <property type="protein sequence ID" value="BAD41463"/>
        <property type="gene ID" value="STH2478"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2478"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_9"/>
    <dbReference type="OMA" id="WKHGIET"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="119"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="217" mass="24449" checksum="531E366808854A74" modified="2004-10-11" version="1">MRSALETQLRDLNRDVLQMGRLVSGMVEKAVRSLAQQDAALAQQVIDQDDEVDRMLIAIEMRCLQVMALQQPMARDLRAVGSSLKVVTDLERVADHATDIAEVTLKLAGEPLIKPLIDIPRMADLAQQMVREALEAFVQQDEAAARTMIARDHDMDDLYRAVFDELVEMMERDPGVVRQATYLLHVAGWLERIGDHATNLGEWIIYQLTGELNDLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ0</accession>
    <name>Q67LJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196594.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2471"/>
    <dbReference type="EnsemblBacteria" id="BAD41456">
        <property type="protein sequence ID" value="BAD41456"/>
        <property type="gene ID" value="STH2471"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2471"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1694627_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="156" mass="17649" checksum="348FD566D1A3E517" modified="2004-10-11" version="1">MVEVRFWRLNDLPWLLHMAAVTDWEIMNPTDRAVARPEVVQANAHRNLLATLASPGGTAFVADDGGRPVGFLLIGIRPDDRTGEWQGYMADIYLLPEYRGKGIAKRLHEAGEWYLRQLGIRRITNWVHATNPLGQKASQARGFRVWGVMMEKLLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ6</accession>
    <name>Q67LJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylmalonyl-CoA mutase C-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2465</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2465"/>
    <dbReference type="EnsemblBacteria" id="BAD41450">
        <property type="protein sequence ID" value="BAD41450"/>
        <property type="gene ID" value="STH2465"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2465"/>
    <dbReference type="eggNOG" id="COG2185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128233_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006159">
        <property type="entry name" value="Acid_CoA_mut_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00640">
        <property type="entry name" value="acid_CoA_mut_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="3">
        <location>
            <begin position="8"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14483" checksum="8083EC0A21BFC4F5" modified="2004-10-11" version="1">MEVATGRRIRVLVAKLGLDAHDRGAKVIARAYRDAGFEVIYTGLYQRPEAVAAAAIQEDVDVIAISSLAGAHGVLIPQLIQLLREQGVDDVLILAGGIIPDDEVPALLEAGVARVFGPGSDLEEIIQYTRENVKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR6</accession>
    <name>Y2395_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>UPF0403 protein STH2395</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH2395</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0403 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LR6"/>
    <dbReference type="SMR" id="Q67LR6"/>
    <dbReference type="STRING" id="292459.STH2395"/>
    <dbReference type="EnsemblBacteria" id="BAD41380">
        <property type="protein sequence ID" value="BAD41380"/>
        <property type="gene ID" value="STH2395"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2395"/>
    <dbReference type="eggNOG" id="ENOG502ZBVN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132521_0_0_9"/>
    <dbReference type="OMA" id="HHIEGRP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009474">
        <property type="entry name" value="YphP/YqiW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40052">
        <property type="entry name" value="PTHR40052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06491">
        <property type="entry name" value="Disulph_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04191">
        <property type="entry name" value="YphP_YqiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000272021" description="UPF0403 protein STH2395">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="140" mass="15020" checksum="48910BFE89625FB0" modified="2004-10-11" version="1">MDLHMFVPDFQAMREELTRLGFEELRTAEEVQEKLPTAKGVTLLAINSMCGCAGGIARPAAALALRQVKPDHLMTVFAGQDKEATAAARALFPQYPPSSPSFAVLKDGQAVAMIPRSQIEGSDPQTVAQRIVEAVQAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY3</accession>
    <name>Q67LY3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative PadR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41313">
        <property type="protein sequence ID" value="BAD41313"/>
        <property type="gene ID" value="STH2328"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2328"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_5_1_9"/>
    <dbReference type="OMA" id="AWVIRIG"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="111" mass="12662" checksum="03DAF4D018B1FD32" modified="2004-10-11" version="1">MYRFVEPVVLFALKRKGESHGYDLAGVVRECALTESEIERASLYRTLKHLEAAGHVTSRWEADQSGPARRLYRLTESGEQYLGEWVETLDRLSQAMARFVAEARSLGEDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY9</accession>
    <name>Q67LY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ssf family Na+/solute symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2322</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196445.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2322"/>
    <dbReference type="EnsemblBacteria" id="BAD41307">
        <property type="protein sequence ID" value="BAD41307"/>
        <property type="gene ID" value="STH2322"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2322"/>
    <dbReference type="eggNOG" id="COG4147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_8_2_9"/>
    <dbReference type="OMA" id="AYMTMVT"/>
    <dbReference type="OrthoDB" id="278483at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019899">
        <property type="entry name" value="Na/solute_symporter_VC_2705"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03648">
        <property type="entry name" value="Na_symport_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="570" mass="60906" checksum="FA4E594052C4BD99" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M40</accession>
    <name>Q67M40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2270"/>
    <dbReference type="EnsemblBacteria" id="BAD41255">
        <property type="protein sequence ID" value="BAD41255"/>
        <property type="gene ID" value="STH2270"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2270"/>
    <dbReference type="eggNOG" id="COG4934">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_314934_0_0_9"/>
    <dbReference type="OrthoDB" id="1281138at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269434" evidence="2">
        <location>
            <begin position="27"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S53" evidence="3">
        <location>
            <begin position="42"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="906"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="759"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="928" mass="101139" checksum="C4705CD31BC6BBE0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M69</accession>
    <name>Q67M69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2241"/>
    <dbReference type="EnsemblBacteria" id="BAD41226">
        <property type="protein sequence ID" value="BAD41226"/>
        <property type="gene ID" value="STH2241"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2241"/>
    <dbReference type="HOGENOM" id="CLU_3066977_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="53" mass="6296" checksum="7F1CE4A56FE3FC0A" modified="2004-10-11" version="1">MVYRTIDQHRLRCALAQFLRFPVRHNPYETVGILHHRHTLRSPTFRDPVAGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M72</accession>
    <name>Q67M72_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Group II intron-encoding maturase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2238"/>
    <dbReference type="EnsemblBacteria" id="BAD41223">
        <property type="protein sequence ID" value="BAD41223"/>
        <property type="gene ID" value="STH2238"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2238"/>
    <dbReference type="HOGENOM" id="CLU_2829690_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="66" mass="7220" checksum="8634472ADE6821BA" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAQAFGRGGGVMMRSGRHVSTWRKGTTGSWTWTWRSSSTGSTTTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA8</accession>
    <name>Q67MA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196325.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2200"/>
    <dbReference type="EnsemblBacteria" id="BAD41185">
        <property type="protein sequence ID" value="BAD41185"/>
        <property type="gene ID" value="STH2200"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2200"/>
    <dbReference type="HOGENOM" id="CLU_1229359_0_0_9"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="23925" checksum="AF9D04FEA5A696F5" modified="2004-10-11" version="1">MPGGGGGGFLQYMDSMAMAFGFLLFLWILHHACGRCVKLHHGAFLSGSMVSLIVVFLVGRKAELALDPFVGAMALLAGLLYATGFLALIDGRRRLTPYFVAYGMVGILGYALAAHYGNVTGAVWQMPLLWMTAIPSGAVLLGGPVWLMSRKGDGSHALWVPLGTILLYAAFYYMVGTTNGTIALGDNASSLINTLLAAGYLCHLLGFVLVRRWLAADRPDAAEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB2</accession>
    <name>Q67MB2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2196"/>
    <dbReference type="EnsemblBacteria" id="BAD41181">
        <property type="protein sequence ID" value="BAD41181"/>
        <property type="gene ID" value="STH2196"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2196"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_1_9"/>
    <dbReference type="OMA" id="VFFNFFN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="339" mass="35781" checksum="F9761BEC3BBF7D62" modified="2004-10-11" version="1">MQETPVTRTQAHPSARFGWILPLLVIAALYALPFLPGLGQNRYLLSLLIGAFVFAVFAMSFDLLLGYTGIVSFGHALFFGAGTYAAALLAKYHGVGFWASLALAPAIAAVLALVIGALSLRVRGVYFAMVSMAFAEFFRVAAEKFSSVTGGSDGLSVQVAPDWAYGPRHRIELYLLTLTLAAVVYLALRRIIHSPFGRVLVAIRENEQRAAAIGYNVFAFKLGAIVLAGAVAAVAGVMYAATENFVVPTVLSTETTINVLLMTIIGGAGTLGGPALGAGVVRLAGTLLSSYTDRWRLALGLLYALIVLFLPHGIAGLARSLRRIAAARAGARAEVRAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG2</accession>
    <name>Q67MG2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196271.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2146"/>
    <dbReference type="EnsemblBacteria" id="BAD41131">
        <property type="protein sequence ID" value="BAD41131"/>
        <property type="gene ID" value="STH2146"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2146"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_7_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="10916" checksum="DD287C3739117088" modified="2004-10-11" version="1">MDLRVGGRYRLSMRLGWGDLVCVGTYREIVPPERLVFTFAWEGDPAGEETVVSLFLREVDGGTELILRHEGFAAPGVAENHRDGWMDCVGRLAALAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG4</accession>
    <name>Q67MG4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD-GYP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196269.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2144"/>
    <dbReference type="EnsemblBacteria" id="BAD41129">
        <property type="protein sequence ID" value="BAD41129"/>
        <property type="gene ID" value="STH2144"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2144"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_1_9"/>
    <dbReference type="OMA" id="YHHERED"/>
    <dbReference type="OrthoDB" id="1094814at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="118"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="311" mass="34678" checksum="32D4188D89440EB6" modified="2004-10-11" version="1">MAKVRRVPVAELQPGDLLARDVLSEDGRVLLSTGVRLNRNYIWQLRQHGVEAVYVPDTSPPKPAPELSLRLRREVTQELRQFTDGLRRTMEQATATVRLPELELDTSGIRRAVVGVVREVLTHPAAALPLQELRQADEYTLLHSVEVCLLSTMIGRAMGLQGSALVDLAMGALLHDLGKVGIPTAILNKPDRLTPEELEVMKRHTTLGWVLLREQPDLPPEVALVALQHHERWDGRGYPMGLAGEQIHLYARICTVADVYDALTADRVYREGIHPTEALATMAGPMREMFDPKVLQTFMNAVEHVQRQGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP1</accession>
    <name>Q67MP1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41052">
        <property type="protein sequence ID" value="BAD41052"/>
        <property type="gene ID" value="STH2067"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2067"/>
    <dbReference type="HOGENOM" id="CLU_2686482_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="7742" checksum="643E0B1B913D50F9" modified="2004-10-11" version="1">MSPGGTSPSPVLRIAHMWGDRAVRNRTAGWMLFSSACLLLGLSLQPVSPNAVLVGFFAGFACALAAVVSYLLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR2</accession>
    <name>Q67MR2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2046"/>
    <dbReference type="EnsemblBacteria" id="BAD41031">
        <property type="protein sequence ID" value="BAD41031"/>
        <property type="gene ID" value="STH2046"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2046"/>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_65_0_9"/>
    <dbReference type="OMA" id="GMQVAKI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008327">
        <property type="entry name" value="Sig_transdc_resp-reg_antiterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036382">
        <property type="entry name" value="RR_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="ANTAR" evidence="5">
        <location>
            <begin position="138"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="23696" checksum="79C2EAA9A9460877" modified="2004-10-11" version="1">MPPTARSRGGNARMRPVRLVVAVHHPEERTRLSAALTGPDRVVVGTTGNGPAAISLVRQLRPDVALVDCRLEGLDGLAAARQLLLDRCAAVVVMIQLHHLSRAYDLIELGAAGCLIKPCSPEQVGLAVLAAARRREEWLTLARRAETAERRLAERRHVERAKALLLEQENLSEEEAYRRLRQLSMRTRRTMGDVAHTLLLNASLRPSRGAENPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N22</accession>
    <name>Q67N22_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SIS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196063.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1936"/>
    <dbReference type="EnsemblBacteria" id="BAD40921">
        <property type="protein sequence ID" value="BAD40921"/>
        <property type="gene ID" value="STH1936"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1936"/>
    <dbReference type="eggNOG" id="COG4821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089975_0_0_9"/>
    <dbReference type="OMA" id="FHPIVEL"/>
    <dbReference type="OrthoDB" id="1605411at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05013">
        <property type="entry name" value="SIS_RpiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035472">
        <property type="entry name" value="RpiR-like_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="34"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="26486" checksum="0B2A70039A5663D6" modified="2004-10-11" version="1">MTITAERFYQAADEAIARLKATQLPAIKRAGELLAETVMKGGVIQVFGTGHSRAFALEMAGRAGGLVPAHAIEEDILFLNGVYPVEERKNPYLEREPEIAHQILAQYDIRPEDIFIIVSNSGRNGSTVEMAAEVKRRGHKLIVVTSMAHTTSVTSRHPSGKRLYEFADVVIDNCGPLGDALLEVEGSPVKVCAVSSITGALIAQGLTAEVIGRLVAAGQTPPVYISANVDGSDEHNEALKARYAGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA1</accession>
    <name>Q67NA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stage III sporulation protein AF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spoIIIAF</name>
        <name evidence="4" type="ordered locus">STH1857</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195985.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1857"/>
    <dbReference type="EnsemblBacteria" id="BAD40842">
        <property type="protein sequence ID" value="BAD40842"/>
        <property type="gene ID" value="STH1857"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1857"/>
    <dbReference type="HOGENOM" id="CLU_1277086_0_0_9"/>
    <dbReference type="OMA" id="DMILPSR"/>
    <dbReference type="OrthoDB" id="1455925at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014245">
        <property type="entry name" value="Spore_III_AF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09581">
        <property type="entry name" value="Spore_III_AF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02896">
        <property type="entry name" value="spore_III_AF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="22856" checksum="C7A7CE3583004734" modified="2004-10-11" version="1">MAALTEWVRGLVVLVVLASLLEMLLPMGGMKRFVRLAMGLVIMLGIVRPILGLLGGQVAVDPEPWLEPTTSLPSVSEIARQAERFQARTQALLLEELQDRIRRAAEEAARSVEGVAEAAAAVELAGGPRLETVSLERVTVTVVLGSRFGQVRPVAPVRIGGEEQAAGGGAGTRARAPTPAETPLAETVRRQVAEQLGLTDASQVTVWIESVDAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE1</accession>
    <name>Q67NE1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40802">
        <property type="protein sequence ID" value="BAD40802"/>
        <property type="gene ID" value="STH1817"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1817"/>
    <dbReference type="HOGENOM" id="CLU_606811_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="451" mass="48534" checksum="3F93230A902BCB90" modified="2004-10-11" version="1">MGAQMAWFGLMYGFSRGQMQLYQGLLVPLFQITPARPLAFLLGRVIEAVPTRAWTTLLWAWAYSAMVPGPGRWPAMALLWTAGLATGMLAHLAGLLLLTFWSRYHPRSMRHGNTAFGVLTIAMMTAAVVYLMEGGTATELALALRAARRSVLTVLTAAAGLPGLMLALALLIRPQWVESHYREGLYRVLELGEQDTVRSGRSWWLPLRGDGVLRAVLSREWLQWVRYRMARTQLLIFAAGVVCVWFAGRSAAGRAMPLAGLVGSVGGLSLLAWYNAFGHWVAKVFQQERITIALYRLAAVPTVRLVIAKLVSVLVPSVLLVAVAAAVGSAAARLPLSTAGSLLLWTELGLVFGVLGGFGAAAATANQEPEEPEAPGAPRMEQGGSAMVQSSAWSSLARTFGLTVSTALPLWAAAGRPWLDLPPASAWTIALGVPLALFLGGVGWMLRTWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE2</accession>
    <name>Q67NE2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1816</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1816"/>
    <dbReference type="EnsemblBacteria" id="BAD40801">
        <property type="protein sequence ID" value="BAD40801"/>
        <property type="gene ID" value="STH1816"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1816"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="DEYLTHC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="11"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28555" checksum="06FE53D6C41BF914" modified="2004-10-11" version="1">MRTTVATEPAILLENLTKSYNGHPAVQGLNLAVRPGEIFGFLGPNGAGKTTTMKMMVGLLPPTSGRVVILGHDVWRNPGPAKRAIGYVPDTPILHELLTAREFLWFMADLYGMSRAEGKARAEELLQMMGLTPQADRLIRDFSLGMKRKMAIATALLHRPRVLLLDEVTNGLDARAAREVKDLIRAWARAGVAVVLTTHVLSVAEELSDRIGLIHRGRLIAEGTAADLARQAGIPGANLEETFLALTGDELPAEDRDTEGEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG4</accession>
    <name>Q67NG4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195922.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40779">
        <property type="protein sequence ID" value="BAD40779"/>
        <property type="gene ID" value="STH1794"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1794"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="SHINCDA"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49929" checksum="7A3395CF0EBE3FEA" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRAEWERIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRCLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWAVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH0</accession>
    <name>Q67NH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1788"/>
    <dbReference type="EnsemblBacteria" id="BAD40773">
        <property type="protein sequence ID" value="BAD40773"/>
        <property type="gene ID" value="STH1788"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1788"/>
    <dbReference type="eggNOG" id="COG3870">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143974_1_0_9"/>
    <dbReference type="OMA" id="KGYRMTE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010375">
        <property type="entry name" value="CdAMP_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38456">
        <property type="entry name" value="PTHR38456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06153">
        <property type="entry name" value="CdAMP_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="9863" checksum="DE4D23C5A42D7925" modified="2004-10-11" version="1">MIVAVVRDEYAGPLTDALTRKGLGATKLSSTGGFLKAGNTTFLIGVRDDQLEEALDAIRGACPGAQVGQGSLKGGKTAPQSSATVFILGVERMLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK5</accession>
    <name>Q67NK5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DinB_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195881.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40738">
        <property type="protein sequence ID" value="BAD40738"/>
        <property type="gene ID" value="STH1753"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1753"/>
    <dbReference type="HOGENOM" id="CLU_1609935_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="1">
        <location>
            <begin position="17"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="17854" checksum="7E668A2F8865E319" modified="2004-10-11" version="1">MIAEARACSEQLAFGLRTIQGAIRDLTPEQLAFVPPGLANSIATLVLHVAATEVVVAEQVLGRRAAEDLRRAVLLDRYTPAPGAPIPAADPGETAESLIGKLERARAEVLGALEALTPADLERTFPFRPGGRPQPLTFFLQLLPFHIASHYGQIALIKRFLRQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN3</accession>
    <name>Q67NN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40710">
        <property type="protein sequence ID" value="BAD40710"/>
        <property type="gene ID" value="STH1725"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1725"/>
    <dbReference type="eggNOG" id="COG3039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070817_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
            <begin position="136"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="32070" checksum="FD8BCEFBBEAF553A" modified="2004-10-11" version="1">MVGLAAKTTKTAGMVARPVYHAVALILQHLHRLLDEVAAERRGRKPRNQPKTGRPRLYSTPSLLLFRIAADLLGWSLRKTLNYAEQEAGAWLRKALGWEQVPHLSVVCRRLHGDDVRRLRTRLWRKLRRRSLRQGDLRVLTIDMTDLPVPLRRKDPDATWGATSKGKFFGYKLHLVISRTGFPLAFRVTPAAVHEMRPAVPLFAEVVKLADQKQKAELTYGVGDSAYDSENLYKFWADVCCGHFLCAPNRRSTPEGEEVRAQGPYRKAALEFWKTDLARRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU1</accession>
    <name>Q67NU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1667"/>
    <dbReference type="EnsemblBacteria" id="BAD40652">
        <property type="protein sequence ID" value="BAD40652"/>
        <property type="gene ID" value="STH1667"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1667"/>
    <dbReference type="HOGENOM" id="CLU_2345621_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="97" mass="10853" checksum="348AAFFA8E5297D4" modified="2004-10-11" version="1">MTLGVTGVQRPAEGLDWREERDREAVEAFQARHRRLADAVERVLMRLVVLGLVILALAQMVGMDRFGLLAALEGTPVHEVTDWSRSLARTANIPYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV4</accession>
    <name>Q67NV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195783.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1654"/>
    <dbReference type="EnsemblBacteria" id="BAD40639">
        <property type="protein sequence ID" value="BAD40639"/>
        <property type="gene ID" value="STH1654"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1654"/>
    <dbReference type="eggNOG" id="COG3415">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041125_3_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="175" mass="19481" checksum="14BA959C49B96E7B" modified="2004-10-11" version="1">MIFVRELSAEERAELERAARRRHRVVSSRRAAIVLASAQGLTAPQIAQRFHCSVDHVRHVIHHFNEHGLDALKPAYCGGRPRVFDANQRARIVKLALTPPQVAGFPWPCWSLSRLQEALVQLHIVDGISKETIRLVLDEAGVRCRSRGAWRLAGRRARKGATHAAPGDSGELPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV7</accession>
    <name>Q67NV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1651</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1651"/>
    <dbReference type="EnsemblBacteria" id="BAD40636">
        <property type="protein sequence ID" value="BAD40636"/>
        <property type="gene ID" value="STH1651"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1651"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="VEPGQFC"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="15"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="29228" checksum="72EECEF260C0D905" modified="2004-10-11" version="1">MRQPVRGEEMRHVKLQIRGLTATYRTARGTITALNGIDLDVREGEKVVILGPSGCGKSTLLKIVAGFERAAAGSVCVDGLPVSGPGPDRAFVFQEFDQLFPWLTVSGNLSFALRVARGVTGREAERAIDEVLSLVGLSRWRDLYPHQLSGGMKMRVAIARALVLNPAVLLMDEPFAALDAQSRSLLQAEVNRMLAETGQTLLFVTHSIEEALVLGDRIVVLSASPGRVRQIIEPAVTGVTLLERPEGAELRRVIRQLLEQDRVAGEMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P55</accession>
    <name>Q67P55_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1553"/>
    <dbReference type="EnsemblBacteria" id="BAD40538">
        <property type="protein sequence ID" value="BAD40538"/>
        <property type="gene ID" value="STH1553"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1553"/>
    <dbReference type="eggNOG" id="ENOG502ZSYS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1703344_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16237" checksum="5C6ED9B4B120D7CA" modified="2004-10-11" version="1">MKAGRVPDGLLSPRCRGHDGSNAGVAPMPAPPVNRGDGRPVFMAKGGSAMRKIGLGLLILLACAPALYWAPWLSADAAQQRAEASFTSGWTGVVDGCGINCQGCGSVGAERVPFGWRVELEYACGLLPADLPEHHRRTVLFVSAFGTVHRVNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P62</accession>
    <name>Q67P62_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dipicolinate synthase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1546"/>
    <dbReference type="EnsemblBacteria" id="BAD40531">
        <property type="protein sequence ID" value="BAD40531"/>
        <property type="gene ID" value="STH1546"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1546"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118958_0_0_9"/>
    <dbReference type="OMA" id="AKAHLRN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014214">
        <property type="entry name" value="Dipicolinic_acid_synth_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001390">
        <property type="entry name" value="Dipicolinate_synth_subunit_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02852">
        <property type="entry name" value="spore_dpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="7"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="199" mass="21783" checksum="72364BCE88DB6CB6" modified="2004-10-11" version="1">MQQLAGKRIGVAMSASHCNLGRAMAQLRRLRDEGAEIIPIISTNVRTTETRFGKPDDWITQIEQITGRLPLMTIPEVEPFGPQVKLDCLVVMPCTGNTMAKLANAINDTPVCMAAKAQMRNHRPVVLAVTTNDGLGMNARNLGALLVARNIYFVPFRQDDPFGKPTSIDADIEHYLVPTILAAMEGRQVQPLLLGPKQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-21" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD7</accession>
    <name>RL19_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplS</name>
        <name type="ordered locus">STH1471</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195601.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PD7"/>
    <dbReference type="SMR" id="Q67PD7"/>
    <dbReference type="STRING" id="292459.STH1471"/>
    <dbReference type="EnsemblBacteria" id="BAD40456">
        <property type="protein sequence ID" value="BAD40456"/>
        <property type="gene ID" value="STH1471"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1471"/>
    <dbReference type="eggNOG" id="COG0335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103507_2_1_9"/>
    <dbReference type="OMA" id="TITVYYE"/>
    <dbReference type="OrthoDB" id="1698718at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000163551" description="50S ribosomal protein L19">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <sequence length="121" mass="14053" checksum="AE7F71886500F721" modified="2004-10-11" version="1">MSDIIREIEREYMRSDIPAFRPGDTVRVNVKVVEGNRERIQAFEGVVIKRQGSGINETFTVRRVSYGVGVERTFPVHSPRLASIEVIRRGVVRRAKLYYLRERTGKAARIRERRLARPEEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-05-10" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD8</accession>
    <name>TRMD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmD</name>
        <name type="ordered locus">STH1470</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195600.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PD8"/>
    <dbReference type="SMR" id="Q67PD8"/>
    <dbReference type="STRING" id="292459.STH1470"/>
    <dbReference type="EnsemblBacteria" id="BAD40455">
        <property type="protein sequence ID" value="BAD40455"/>
        <property type="gene ID" value="STH1470"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1470"/>
    <dbReference type="eggNOG" id="COG0336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047363_0_1_9"/>
    <dbReference type="OMA" id="ILCGHYK"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000060479" description="tRNA (guanine-N(1)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <sequence length="247" mass="27517" checksum="36A88ED025CADA28" modified="2004-10-11" version="1">MLIQILTIHPAIVAPVFRESILGRACEAGILDLRVVNIRDFALSKHQQTDDYPYGGGAGLLMKPEPVFGAVRWAAGRAPAGARPPRVILMDPQGRRFDQRYAEELAREDHLILICGRYEGFDERIRALATDEISIGDYVLMGGEVAALVVVEAVTRLIPGVLGDLESSVAESHTSGLLEGPQYTRPAEFEGMRVPEILTSGNHGAIARWRREQALRRTFERRPDLLQSADLTPEERRLVEAWRTRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT1</accession>
    <name>Q67PT1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1327</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 manganese ions per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1327"/>
    <dbReference type="EnsemblBacteria" id="BAD40312">
        <property type="protein sequence ID" value="BAD40312"/>
        <property type="gene ID" value="STH1327"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1327"/>
    <dbReference type="eggNOG" id="COG1690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022279_1_0_9"/>
    <dbReference type="OMA" id="QTRGVEC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008452">
        <property type="term" value="F:RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="SSF103365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="1">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="323"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="350"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="445" mass="48343" checksum="BABCA734F859412A" modified="2004-10-11" version="1">MCAGRGASREEVRRGAANQGTRESGGCVAMPFTLWERYPVFGEHDRDTLAQLGRCAEAGRAVGAALMADGHKGYSQPIGGVLFYERFVSPSGVGYDIACGNKAVRTDLVYGEIRREKDRIAAEIHRRVAFGLGRKNPDPVDHELFDDPTWRDVRPLQSLKSMARDQLGTVGAGNHYVDIMAEVPEGWQLGDPIADDAPVWVAVHFGSRGFGHRTASGFLNLAAGRPWDAGAPGESMDQPPTLIPADSELGQAYIAAMKLAGRYAYAGRDYVVDQVLRILGARPTFTVHNHHNFAWFEEHGGVKGWVIRKGATPLFPGQLGFIGGSMGDVAVVVQGVDHPESRAALYSTVHGAGRVMSRTQAAGRWKRHRGRRIRTGGAVDMDAVRRRLAQAGVALVGGDADEAPQVYRPLRQVLRWHAPTLKVLHVLAPVCVVMAAADEFDPYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW0</accession>
    <name>Q67PW0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1298"/>
    <dbReference type="EnsemblBacteria" id="BAD40283">
        <property type="protein sequence ID" value="BAD40283"/>
        <property type="gene ID" value="STH1298"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1298"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_3_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="199" mass="22395" checksum="0AC029892C0F90EB" modified="2004-10-11" version="1">MRPLPPQRYEFAEWRKAKVNLDYHVEVDRNYYSVPYPLVGETVDIRLTDGLVEILHRGKRVACHSRATGRGRSVTQIQHMPASHRRHREWTPSKLRRWAESVGPHTAQLVVTILEEKPHPEQGFRSCLGIIHLARKYGSDRLEAAAARACAARTFSYSSVKSILAHNLDQAPLPGPPDNQATPVHPNLRGPDYYAGGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW5</accession>
    <name>Q67PW5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195424.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1293"/>
    <dbReference type="REBASE" id="10276">
        <property type="entry name" value="StpMcrB2P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40278">
        <property type="protein sequence ID" value="BAD40278"/>
        <property type="gene ID" value="STH1293"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1293"/>
    <dbReference type="eggNOG" id="COG1700">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018970_0_0_9"/>
    <dbReference type="OrthoDB" id="643928at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018633">
        <property type="entry name" value="DUF2357"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007505">
        <property type="entry name" value="PDDEXK_7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09823">
        <property type="entry name" value="DUF2357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04411">
        <property type="entry name" value="PDDEXK_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2357" evidence="1">
        <location>
            <begin position="16"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="515" mass="58945" checksum="2D0541326789025F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX7</accession>
    <name>Q67PX7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1281"/>
    <dbReference type="EnsemblBacteria" id="BAD40266">
        <property type="protein sequence ID" value="BAD40266"/>
        <property type="gene ID" value="STH1281"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1281"/>
    <dbReference type="HOGENOM" id="CLU_2332603_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="10558" checksum="E18A5C8DD513F3BC" modified="2004-10-11" version="1">MPKRSGEGFAGTRNRPGGSPAFAVPGSRARRSARRWARGVWVGCTICSHPVLAEAAPVSVWSVRIEYGSERCPGSVQSLQNELLFGVNLGGVRFTRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY2</accession>
    <name>Q67PY2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PTS system mannose-specific IIB component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1276</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195407.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1276"/>
    <dbReference type="EnsemblBacteria" id="BAD40261">
        <property type="protein sequence ID" value="BAD40261"/>
        <property type="gene ID" value="STH1276"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1276"/>
    <dbReference type="eggNOG" id="COG3444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116175_1_0_9"/>
    <dbReference type="OMA" id="MVPNESK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.35.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004720">
        <property type="entry name" value="PTS_IIB_sorbose-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036667">
        <property type="entry name" value="PTS_IIB_sorbose-sp_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03830">
        <property type="entry name" value="PTSIIB_sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52728">
        <property type="entry name" value="SSF52728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51101">
        <property type="entry name" value="PTS_EIIB_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIB type-4" evidence="8">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17111" checksum="5AF7D1D8BB4BC0CC" modified="2004-10-11" version="1">MPIVLVRVDDRLIHGQVAVGWTRTVGANHIVVANDEVARDATQKSLLKLAAPVGVKVSILSVAEAAALLVGPKAAKDRVMVLVRDPQSLLALMDGGVELTKVNVGNVRAAEGRVRLTKEVAASPEEIEAWKELDRRGVILEAVWLPGGAVTDFNSVIRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ2</accession>
    <name>Q67PZ2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195397.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1266"/>
    <dbReference type="EnsemblBacteria" id="BAD40251">
        <property type="protein sequence ID" value="BAD40251"/>
        <property type="gene ID" value="STH1266"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1266"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137371_1_0_9"/>
    <dbReference type="OMA" id="FTKRIVH"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="38"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="12647" checksum="DAC5BB5497AB8C33" modified="2004-10-11" version="1">MAGVIATEQTNLAAVSLLSEARFTKRICFSNPQVLVFVLTFAPGQELPKHRHPGSTLVVHVTGGSGVVVADGRETPVAAGDILLVEGEEEFAVRNTGSEALVLLASLSPNPSDPRYAQPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q07</accession>
    <name>Q67Q07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PTS system N-acetylgalactosamine-specific IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">agaF</name>
        <name evidence="5" type="ordered locus">STH1251</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195382.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1251"/>
    <dbReference type="EnsemblBacteria" id="BAD40236">
        <property type="protein sequence ID" value="BAD40236"/>
        <property type="gene ID" value="STH1251"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1251"/>
    <dbReference type="eggNOG" id="COG2893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123235_1_0_9"/>
    <dbReference type="OMA" id="RDGIVHK"/>
    <dbReference type="OrthoDB" id="2008912at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00006">
        <property type="entry name" value="PTS_IIA_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033887">
        <property type="entry name" value="PTS_IIA_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-4" evidence="4">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="15589" checksum="FBBA2F5663891EAD" modified="2004-10-11" version="1">MIGCIVTGHGEFAGGLAQALTMIAGEQEHFELVPFRETEPLDGFAERMKAALQRLLGQTDGVLIFTDLLGGTPFRTAMLAAAGHENVAVLTGTNLPMLIEIGLMRSAQSDVQALARTAEEAGRAGIQAARLEPGGSAPPSGAGSDDDSEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q41</accession>
    <name>Q67Q41_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small basic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sbp family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195348.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1217"/>
    <dbReference type="EnsemblBacteria" id="BAD40202">
        <property type="protein sequence ID" value="BAD40202"/>
        <property type="gene ID" value="STH1217"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1217"/>
    <dbReference type="eggNOG" id="COG3856">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135532_1_0_9"/>
    <dbReference type="OMA" id="TGFFFNI"/>
    <dbReference type="OrthoDB" id="1930757at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009709">
        <property type="entry name" value="DUF1290"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06947">
        <property type="entry name" value="DUF1290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018579">
        <property type="entry name" value="Sbp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR018579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="128" mass="14167" checksum="EEFA2A311635D754" modified="2004-10-11" version="1">MWIIIVGFIAGILIGIFSPFTLPVAYARYLSIAVLAALDTSFGGLRASLEGTYDNAIFITGFFTNTLLAAGLVYLGDRIGVDNLYLAGVVAMGIRMFQNLGIIRRLILQRWGFFHRDEETLQHHGLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q70</accession>
    <name>Q67Q70_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lactoylglutathione lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylmalonyl-CoA epimerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195319.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1188"/>
    <dbReference type="EnsemblBacteria" id="BAD40173">
        <property type="protein sequence ID" value="BAD40173"/>
        <property type="gene ID" value="STH1188"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1188"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_5_2_9"/>
    <dbReference type="OMA" id="IHHICYE"/>
    <dbReference type="OrthoDB" id="1335734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07249">
        <property type="entry name" value="MMCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017515">
        <property type="entry name" value="MeMalonyl-CoA_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03081">
        <property type="entry name" value="metmalonyl_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="16371" checksum="8BC88715FB22E560" modified="2004-10-11" version="1">MMMEIDHIGIAVRDLQAQIALYRDRLGLRFEGVEEVPAQKVRVAFFAAGGARVELLESTAPDGPIARHIERRGEGLHHVAYRVADIRAAMAEARAQGMELVDQEPRPGAHGALVCFLHPRSTGGVLTELVQHVGSEWQREHGGPGGGASRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG8</accession>
    <name>Q67QG8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PepSY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1090"/>
    <dbReference type="EnsemblBacteria" id="BAD40075">
        <property type="protein sequence ID" value="BAD40075"/>
        <property type="gene ID" value="STH1090"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1090"/>
    <dbReference type="eggNOG" id="COG2959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045803_0_0_9"/>
    <dbReference type="OMA" id="WRITSEA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014239">
        <property type="entry name" value="Spore_YpeB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03413">
        <property type="entry name" value="PepSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14620">
        <property type="entry name" value="YPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02889">
        <property type="entry name" value="spore_YpeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PepSY" evidence="1">
        <location>
            <begin position="378"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="451" mass="48934" checksum="EF2A384A50480DFB" modified="2004-10-11" version="1">MLAVWGAWTVHLYRQYQALAYELEAERQRNFAEMISHVEAMRGLMGKSLAAGSTRQNALYMGEVYRRASLAAANFMALPLPEELGAATGKFLNQIGDFAYSVVRHEAAGRTMDEAQRQELARLYQAATDLTATLRDTGQASVSEGFRFAKAGVGLSDLFTAWRERRAAGGADLTQDQAQKSLIPPGLNQVGPQMDQMPVLVYDGPFSDHLEQRTPAMGGPAITPEEARARALAFLPEGVTADALEVTERNGRVPVFAVRLPPGGGRPAVTVDLAREGGHLVSYINARPAGEPRLTLEDAREAGLAYLAAHGWSEMEPTYGEVADGFATVQFVHAPGGVRIYPDQVKVRVALDNGEVVGVDARSYVMSHRERGGLTPSVTREQARAAVNPELQVEEARLALIPTEAGDGEVLCWEFRGTLGEETYLVYVNAHTGLEERILQMLITDSGTLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH8</accession>
    <name>Q67QH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endo-1,4-beta-glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1080"/>
    <dbReference type="EnsemblBacteria" id="BAD40065">
        <property type="protein sequence ID" value="BAD40065"/>
        <property type="gene ID" value="STH1080"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1080"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_0_2_9"/>
    <dbReference type="OMA" id="FMAHMDE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="342" mass="37142" checksum="45CBA025E82CB732" modified="2004-10-11" version="1">MLLERLSNACGVSGDEGEVRALLKEVLAPRVDEMWSDPMGNLIVRKGSGPVRVMLDAHMDEVGFLVGEITDEGYLSLRKVGGLDDRVLPGRQVWVTHRRIPGVIGAKAWHLCSPEERGKVIPFDEMYVDLGCRSRAEVEALGIELGDPVYFATEFELLGDRVAKGKALDDRVGCTVLARVLLEYDFPGITLLGAFTTQEEVGLRGAKAAAFHLNPDVALALEGTGSANVAGVDPFDTITNMGEGPVISLMDAAGIPHKRVQEELIRLARKHGIRHQFRRLTSGGTDAGAIARSRQGVPACTISVPCRYIHTNAALCHLDDVDGAVQLVRRFLESVEKGEFRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK1</accession>
    <name>Q67QK1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1057"/>
    <dbReference type="EnsemblBacteria" id="BAD40042">
        <property type="protein sequence ID" value="BAD40042"/>
        <property type="gene ID" value="STH1057"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1057"/>
    <dbReference type="HOGENOM" id="CLU_2014102_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13887" checksum="CCB125298432B448" modified="2004-10-11" version="1">MRGKQQGVRSGRPAQCEVTSVAKAGVDRRREETRTPTCSVLVFSCFGARRRRATVVSVRQGLSRDVGRFWRSDSAMESRAFTDRLASTSTHMRARVIPLPRLTLPATPGFHCRRARERLMPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM4</accession>
    <name>Q67QM4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="3">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bipA</name>
        <name evidence="5" type="ordered locus">STH1034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195166.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1034"/>
    <dbReference type="EnsemblBacteria" id="BAD40019">
        <property type="protein sequence ID" value="BAD40019"/>
        <property type="gene ID" value="STH1034"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1034"/>
    <dbReference type="eggNOG" id="COG1217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017016_4_0_9"/>
    <dbReference type="OMA" id="MSMLFTI"/>
    <dbReference type="OrthoDB" id="143460at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="4"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="619" mass="69063" checksum="8926147A6F9E8433" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP0</accession>
    <name>Q67QP0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195150.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1018"/>
    <dbReference type="EnsemblBacteria" id="BAD40003">
        <property type="protein sequence ID" value="BAD40003"/>
        <property type="gene ID" value="STH1018"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1018"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_972968_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="285" mass="30963" checksum="B3FD6B663D1BD940" modified="2004-10-11" version="1">MGKEVEALFEDVHVHAELAHVLQQEGRWAEALHHARRVVALAPRGSVLHLWGYAKAATIAADMGRGQLAHRFATVYLALARGASGVEQYTPWVQQAMGIACYQRGRFQEAIAWYRRAVQGFARCGLTEQVAVASSMLAFNLARIGRPQRARACLADRSEFPESRSYLYDSAMTAIHMAEGDHELAVSSGRRALTASGRRSFDFADAAAVALLVSRALRALGARREATAFILSAARFAVRQRWEVLAPLLPNRGQRGGDMAREAAVSRGRGSAHPRDGASLCPGCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ3</accession>
    <name>Q67QZ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195047.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH915"/>
    <dbReference type="EnsemblBacteria" id="BAD39900">
        <property type="protein sequence ID" value="BAD39900"/>
        <property type="gene ID" value="STH915"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH915"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031960_9_2_9"/>
    <dbReference type="OMA" id="FVTRFMV"/>
    <dbReference type="OrthoDB" id="991474at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="1">
        <location>
            <begin position="26"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="29691" checksum="8CDF9E7FFA72BCA0" modified="2004-10-11" version="1">MTDPVSTLNALVAGFSGTMGVWARSLQTDEVIQVGASDDTFPSASTIKLAIFYEVMRQAGEGRLDLSEPRELRTEDKVPGCGVLRDLSPGIRLPLRDLATLMMIISDNTASNMCIDAVGIESVNRSMDELGLTGLRLRWKFFGAPAGQPVNAAVPSQLGRLMDMIVRRQVLTPEACDEMLRVMKQVQSPYAARYLPGGSQYDRPPEGPVIAAKFGSITGCRHEVGAVWKGGRGFVFSVMTKDCKDERRVEDNEGVLAVGRAVAILYRHFLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R44</accession>
    <name>Q67R44_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell wall hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194996.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH864"/>
    <dbReference type="EnsemblBacteria" id="BAD39849">
        <property type="protein sequence ID" value="BAD39849"/>
        <property type="gene ID" value="STH864"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH864"/>
    <dbReference type="eggNOG" id="COG3773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148732_0_0_9"/>
    <dbReference type="OMA" id="MIYQPHA"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011105">
        <property type="entry name" value="Cell_wall_hydrolase_SleB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042047">
        <property type="entry name" value="SleB_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07486">
        <property type="entry name" value="Hydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_2" evidence="1">
        <location>
            <begin position="11"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="134" mass="15263" checksum="B4298A9083CA6E43" modified="2004-10-11" version="1">MARLMRAEAEGEGDLGMLMVGNVITNRVYANCGEFQGLRSVRDIINRDLATECRTGFEAVCKPYFWQGARERDIRLAERNARGEQQHPATYALWFFRPPGQACPATWWGRPLAGQYKNHCFYLADAQACPRVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R54</accession>
    <name>Q67R54_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194986.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH854"/>
    <dbReference type="EnsemblBacteria" id="BAD39839">
        <property type="protein sequence ID" value="BAD39839"/>
        <property type="gene ID" value="STH854"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH854"/>
    <dbReference type="HOGENOM" id="CLU_1748731_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025531">
        <property type="entry name" value="DUF4418"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14387">
        <property type="entry name" value="DUF4418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="15884" checksum="0CA60D2131B8E3C5" modified="2004-10-11" version="1">MSKLTRDWRPLLFIVLGAGAVLVSHFFLHTCTDHGHFMETAAGTLVPMRCHWSERAFQGVGALVAFAGLAMYVFPDAARGLSFAVAGAGLLMILIPMWLVPTCDMPGMVCNLSFKPGAYLTGGITALSGLGGTLRLRRLDVMGGERSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG3</accession>
    <name>Q67RG3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH745"/>
    <dbReference type="EnsemblBacteria" id="BAD39730">
        <property type="protein sequence ID" value="BAD39730"/>
        <property type="gene ID" value="STH745"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH745"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_9"/>
    <dbReference type="OMA" id="ENDHQIN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="22"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="127"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="222" mass="25273" checksum="9E7921ADD337C39C" modified="2004-10-11" version="1">MCAVAPRSAFDEQLAHLQRDMLRMAVFVGEAIEKAVQSLQKADRNLARAVVDGDEVADRMAIDLQQRCLQLMALQQPMARDLRAVGTVLKVVTDLERMADHATDIAKVVLRLERPVPQGLLFDIPLLARRVQEMNREALDAWVQGDSDRARRMTRRDDEIDRLYRKVFDDLLQAMQSDPKAVRPAVYLLLVAHYLERIGDHATNLGEWTIYQETGELQDLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG5</accession>
    <name>Q67RG5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39728">
        <property type="protein sequence ID" value="BAD39728"/>
        <property type="gene ID" value="STH743"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH743"/>
    <dbReference type="HOGENOM" id="CLU_1073352_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="25789" checksum="88AB16B3C452F556" modified="2004-10-11" version="1">MTPPLGPSSARRASPGPGSRAKSEPGLCASGQILRVNPVPAPGSRAKPEPGLCASGQILRVNPVPAPGSRAKSEPGLCASGQILRVNPVPVPGARAKSEPGLCASGQILRVDSAAAPGSRAKSEPGLCASGQILRVDPVPAPGSRAKSEPGLCASGQILRVDPVPAPGSRAKSEPGLCASGQILRVDPVPAPAPAPSRLAAYSPAEVEQREAPGVTRPRSPGTPVEARLGPGTGAFTCGIGTPDLAWTVSPCAQNLGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM5</accession>
    <name>Q67RM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Carbon starvation-induced protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH683</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptide transporter carbon starvation (CstA) (TC 2.A.114) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF13722"/>
        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <sequence length="591" mass="64115" checksum="8702D820E50D0214" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM6</accession>
    <name>Q67RM6_SYMTH</name>
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        <submittedName>
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        </submittedName>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
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        <location>
            <begin position="42"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM8</accession>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
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                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="334"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="38912" checksum="DF668356DB1DD882" modified="2004-10-11" version="1">MWREMAQTLREFLDSRPNLRYIFTGGKGGVGKTVTAAVLAYQFALEGKKTLVASLNPVHSLTSVFGQNLSGGQFRQVEGVPNLWAVEVDASDVVARYRENIAKRVREFLKYADIPVDAGPFVDIAVTNPAFEESAMFDKMIDVMLNEARDFDRIVFDTAAVANAIRLIGLSKIYGLWLSRMIQSRKEALSLRVQLSFRKEKVEEEVRKDPMLADLLDMNDRFTRVKKLLVDPEVTAFFFVTLPLTLPISVVKRFIEQVRAYDIPVGGVLVNSCIRADEARRAAGDEYLQNKFREQMGYMQVIEQDLGPLVRSYIPLYKSEVHGLDALKQVARDMFAPADAALYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR1</accession>
    <name>Q67RR1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH647"/>
    <dbReference type="EnsemblBacteria" id="BAD39632">
        <property type="protein sequence ID" value="BAD39632"/>
        <property type="gene ID" value="STH647"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH647"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_0_0_9"/>
    <dbReference type="OMA" id="AHARYNP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="39152" checksum="C88B7C103DDB3396" modified="2004-10-11" version="1">MANQLWQVLMLNVRQQMRNKSAFFFNLIMPVLMLLFFGATNAGSDTVTAVTVGLVDRDGGPVAQLIRTGLQESGYFEVEAGEEAELIRRLDAGKVRAVLVLPAGLSEQVAVGSGPAEVIVHWDPTSTASAAAQGGLQYLIQGLETFQKEPSIAVRDQRLDSTEQLGLFDFMMPGLLVYMTLNAGVLAIGTAVSYQQRAGTLRHMFSTPLTMGAWLVGTVLGNHLLAILQLSVIWCVGIAFFKIQLPNNLPGTVAILLLSSTAGIGIGLAIAAFARSGETAMPIAIIASMGLTFLGNAMMPLDHAPEIVQTVMRFMPSYYMTHALQQVTMKGQPLAAVLPDLGVLALTATIGLGLAAWRLRRTVVTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS2</accession>
    <name>Q67RS2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH636"/>
    <dbReference type="EnsemblBacteria" id="BAD39621">
        <property type="protein sequence ID" value="BAD39621"/>
        <property type="gene ID" value="STH636"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH636"/>
    <dbReference type="eggNOG" id="COG3876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033227_1_0_9"/>
    <dbReference type="OMA" id="PGMCLFE"/>
    <dbReference type="OrthoDB" id="1439085at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033922">
        <property type="term" value="F:peptidoglycan beta-N-acetylmuramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008302">
        <property type="entry name" value="NamZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42915">
        <property type="entry name" value="PTHR42915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07075">
        <property type="entry name" value="DUF1343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016719">
        <property type="entry name" value="UCP016719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="388" mass="42884" checksum="E6BD6CEA0AB216E4" modified="2004-10-11" version="1">MIGLADVRLGNEVLLRSRRQLLSGVRVGIVTNHTGVNSRLESIIDLLAADPGVRVTALFSPEHGIRGDVPAGEKVDRAVDPATGLPVHSLYGDTRKPAPEMLADVDVLLFDLQDAGVRFFTYTWTMALCMQAAAEQGKRLIVLDRPNPIGGLQVEGGIVQPGLESFVGLYPVAIRHGLTHGEIATWLNQRFKIGCDLVVVPLEGWRRSMWWDETGLPFVPPSPAANGLDMLTLYPGLCFLEGTNLSEGRGTALPFQVFGAPWLDERRTVAELRALELPGVLFRPAYFIPTASKHQGRLCRGVQVHIVDRRVVRPVELGAHVIAVVRRLHPKHFEWVMWGSTRWLDRLSGSDRLYRAAEGELPLGQVLSAWAEEARSFAEEAAAYRLYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU8</accession>
    <name>Q67RU8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH610"/>
    <dbReference type="EnsemblBacteria" id="BAD39595">
        <property type="protein sequence ID" value="BAD39595"/>
        <property type="gene ID" value="STH610"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH610"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093397_0_0_9"/>
    <dbReference type="OMA" id="KWRRECK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="269" mass="29353" checksum="65DC37D4E4609102" modified="2004-10-11" version="1">MLQRNPFHLGRPHGVFGHICRTLAPFVAEPEPEKAVLRRLVALHRARAAAGDMVGCHDWDDIVNTIAREVGFPERIDVAGLVEAYCRMEGMIWAYPGAAECLDRLRAEGHTLVSVTNGFRCYQEPVQRALGLLPRFAALITPDTAGAGKPQPSIYRAAEAYGGPPFIHVGDVLPHDIAGARAAGWLAIYIVQPLAPGYTPMPPALAALPPWQRPAAGRAWLEERFAIDRRWHDYPPCELADCIPDAIVNHLEEVPAAVEHLVRAGPQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX0</accession>
    <name>Q67RX0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor SigA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpoD</name>
        <name evidence="6" type="synonym">sigA</name>
        <name evidence="11" type="ordered locus">STH588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194722.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH588"/>
    <dbReference type="EnsemblBacteria" id="BAD39573">
        <property type="protein sequence ID" value="BAD39573"/>
        <property type="gene ID" value="STH588"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH588"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_3_3_9"/>
    <dbReference type="OMA" id="YQRWLAE"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="9">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="10">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="155"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="234"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="6">
        <location>
            <begin position="323"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="388" mass="43796" checksum="5CF57A024E3D31F6" modified="2004-10-11" version="1">MESNEAKMQNARELVKQLIAKGKRKGSLSYDEVADSLQELELDAEQMEAIFDTLTEAGIKLQGDEPDEAEEPVDGDADVEALDVVEPPPVDDAEPDDDAAAGEDDDLSVPEGIAIDDPVRMYLKEIGRVPLLTAEEEIMYAERIEKGDEEAKRKLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVTRQLVQELGREPTPEEIAAVMDIPVERVREIQKIAQEPVSLETPIGEEEDSHLGDFIEDQDAPAPAEAASFTLLKEQLEEVLESLTPREEKVLRLRFGLDDGRARTLEEVGQVFGVTRERIRQIEAKALRKLRHPSRSKKLKDYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX6</accession>
    <name>Q67RX6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194716.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH582"/>
    <dbReference type="EnsemblBacteria" id="BAD39567">
        <property type="protein sequence ID" value="BAD39567"/>
        <property type="gene ID" value="STH582"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH582"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1694627_0_0_9"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="155" mass="16612" checksum="A7DCD50B6F04565E" modified="2004-10-11" version="1">MAVEVGLLTVAEWPWLVSRSAATAWAHVDPALRDRVNPQAVAARVEQNLRALLTRPGSAALVARAGGAAAGYLVVALVPDELTGLPVGLFYDIFVEPAYRGTGVSHRLTAAGEAHCRAWGASLVRRYIDPGNEPSLRHALREGCRIDRLSLVKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S35</accession>
    <name>Q67S35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH523</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194657.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH523"/>
    <dbReference type="EnsemblBacteria" id="BAD39508">
        <property type="protein sequence ID" value="BAD39508"/>
        <property type="gene ID" value="STH523"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH523"/>
    <dbReference type="eggNOG" id="COG2270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_16_1_9"/>
    <dbReference type="OMA" id="MQLTVQN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="415" mass="43428" checksum="3634484C73A0DB60" modified="2004-10-11" version="1">MTRYLSLLRNRNFLRYWLAGALAHVGDFFNTLALVKLFSQDPDRLGFYTSLVVVGNMLPTLLLGPVAGVLADRLPRKLIMVTADLVRAALMFSMMFVHDPLALIGIDFAAAVAAAFFGPASSALLPRLVQREELATAGSLNIMTQRLAMLFGNGLGAVLLGLVGPAGVFAINTGLFLASGLLHATMRAPETAPAEAGSRPAASPLAKLVRDLREAAAVVRERPNLLHLLAGLCIANFGDSGSNVLLVTFFTVTLGVAAEQLGFVMALFGGAAVLGAFAIGAVGNRVHWRHLFSLGCCYVWFTATGAVVVRSLIPSSAFLLLLGLGSGAINVGLQVAIADLVPDNVRGRIFGSFGTISSLIMIVGTLLAGVLADRFGAAATMLGYALFYLAAGIYGYQKLRPEPAPAAQAASARAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S36</accession>
    <name>Q67S36_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH522"/>
    <dbReference type="EnsemblBacteria" id="BAD39507">
        <property type="protein sequence ID" value="BAD39507"/>
        <property type="gene ID" value="STH522"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH522"/>
    <dbReference type="HOGENOM" id="CLU_754249_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="39314" checksum="600CE38933F59EA9" modified="2004-10-11" version="1">MVRHRHRTGRLRPGGVSLVNLFSARAGPGAPLTLALLALLEHSDRSVLEGFLALAGLSPPEGAEASFHYPAPDGPPGAGEIRLGDRRIRVAAVAPGEPPPEFPPGTAEGLLVGGPAPAGPGGLPGAEEGEPVRRLSWERLDRWLEEAAAVHDPDTRTGFLIRQFRAYLPEAGITWFRGFDAEELDRAPRAFRELSAFHRRVGELFEQVGSGFAASWPLLRTARPEDLLAGYWFRDYAVGSGGGDFLRVALDLGAGELQIALWFEPGGGAHGRLQLALAEEGGLSRALARLTPPPVLRLWSAADERHIPVGDLDPSRIHAVDWEAYTAAVQVARPLTDLAGEGLPDRLAGWTRALLDALAPVLSEVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC2</accession>
    <name>Q67SC2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsfS</name>
        <name evidence="3" type="ordered locus">STH436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH436"/>
    <dbReference type="EnsemblBacteria" id="BAD39421">
        <property type="protein sequence ID" value="BAD39421"/>
        <property type="gene ID" value="STH436"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH436"/>
    <dbReference type="eggNOG" id="COG0799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092688_2_2_9"/>
    <dbReference type="OMA" id="VVHVFQK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="12620" checksum="FE9216ADAF213B10" modified="2004-10-11" version="1">MAQWAAIAAEGKKARDVRILDIRSISVVADYFVICSGTSGTHVRAIVDHVEEELDKKGHKVRHVEGYSGGRWVLLDFGDVVVHVMQEEEREFYNLERLWGDAVEVAVETPAVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI3</accession>
    <name>MINC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable septum site-determining protein MinC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">minC</name>
        <name type="ordered locus">STH375</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with MinD and FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MinC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194509.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SI3"/>
    <dbReference type="SMR" id="Q67SI3"/>
    <dbReference type="STRING" id="292459.STH375"/>
    <dbReference type="EnsemblBacteria" id="BAD39360">
        <property type="protein sequence ID" value="BAD39360"/>
        <property type="gene ID" value="STH375"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH375"/>
    <dbReference type="eggNOG" id="COG0850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048711_0_0_9"/>
    <dbReference type="OMA" id="IYKMAED"/>
    <dbReference type="OrthoDB" id="1665744at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901891">
        <property type="term" value="P:regulation of cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00267">
        <property type="entry name" value="MinC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013033">
        <property type="entry name" value="MinC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036145">
        <property type="entry name" value="MinC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005526">
        <property type="entry name" value="Septum_form_inhib_MinC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34108">
        <property type="entry name" value="PTHR34108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03775">
        <property type="entry name" value="MinC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63848">
        <property type="entry name" value="SSF63848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01222">
        <property type="entry name" value="minC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_1000047869" description="Probable septum site-determining protein MinC">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00267"/>
        </source>
    </evidence>
    <sequence length="228" mass="23989" checksum="5B42A79CBD43822B" modified="2004-10-11" version="1">MRQDIVIRGTTRTGLLLLLPDEGEFSAVLERLAERLASSGRFFVGGRVQVHVGNRRLSPEDREALEQTLQRSGMVLLSVKEGGDPLAEVQAPEAGAPSAPPPPAGNTLVVTKTVRSGQEIRHDGDVIILGDVNPGAVVVATGHIVVMGALRGVAHAGCTGNRTAIVAATKLRPTQLRIAEVIGRAPDGDAPQSYPEVARIRGDLIVVEASAEKRQVSALEAVGAKEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ5</accession>
    <name>Q67SJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH363</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194497.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH363"/>
    <dbReference type="EnsemblBacteria" id="BAD39348">
        <property type="protein sequence ID" value="BAD39348"/>
        <property type="gene ID" value="STH363"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH363"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_18_9"/>
    <dbReference type="OMA" id="CALWIAV"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="6">
        <location>
            <begin position="86"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="5">
        <location>
            <begin position="186"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="500" mass="52910" checksum="DF93F498B7DFC72B" modified="2004-10-11" version="1">MRWSHLINPLQWNFGIRTVATLFTWALLPVFLTTFVAFRRLGAAAQTLGLSAQQLQALESHLLQAVLWVEVPIVIVVIGASILFAYVVVKPLARLKEAMQRVAQGDLSQTSVVVTSRDEVGQATRSYNMMASQLAAMVRTLAQTASNLERAAAEVDRSAREADEVTGASSREIANMETMAARQAEYAADGARAIREVEEAASQVAAAAQSQAEEVERAAATVRQMAEAIEQVAESAGVVAEAATRTREAAEQGGEAVRQSAEGMNRVRDRVLDASNQVRQLSESLQQVDEILQLITEIAGQTDLLALNAAIEAARVGEHGKGFAVVAGEVRRLAERVRTAAGEIAGRIEGLRSSAAVVVQTMEAGTAEVEHGVQLSAEAGASLERILAAVADTQKEAQSISAAAQEMAAASQDVVNATVQLSAIAEENAATAEEMLASAKAVTTLIGQVEEQSQAGRHAMAAMSASATQVQAAMQRLVELAGKASDMANALRQQAAQFRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL6</accession>
    <name>MURI_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate racemase</fullName>
            <ecNumber evidence="1">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murI</name>
        <name type="ordered locus">STH342</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Provides the (R)-glutamate required for cell wall biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194476.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SL6"/>
    <dbReference type="SMR" id="Q67SL6"/>
    <dbReference type="STRING" id="292459.STH342"/>
    <dbReference type="EnsemblBacteria" id="BAD39327">
        <property type="protein sequence ID" value="BAD39327"/>
        <property type="gene ID" value="STH342"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH342"/>
    <dbReference type="eggNOG" id="COG0796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052344_1_0_9"/>
    <dbReference type="OMA" id="MPWGPRT"/>
    <dbReference type="OrthoDB" id="1718671at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00258">
        <property type="entry name" value="Glu_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033134">
        <property type="entry name" value="Asp/Glu_racemase_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00924">
        <property type="entry name" value="ASP_GLU_RACEMASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000078581" description="Glutamate racemase">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="80"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="192"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00258"/>
        </source>
    </evidence>
    <sequence length="277" mass="29860" checksum="83CDE29B5B339D3C" modified="2004-10-11" version="1">MTQYIPDTQRPIGLFDSGEGGLTVARAVADLLPQENLIYACDTAHFPYGPRPLAEVRAFFRRFMEFFVEQNCKLVIVACNTATAAAIDLLLADAFPIPALGVVQPGAAMAAEASVTGRIGVAATQGTCDSGIYPQTIRLFRPDAYVVQQACPILVIRAEEGVISGPEVRREVERCLAPILAERVDTLVLGCTHFPHMAKVIQDVVGPAVRLVDPGKATAVQVADLLRRRGLLNPGPGPGQRRAFTTGDPQRFLEVACRLWPGGVDAAAHIHLWSQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL7</accession>
    <name>Q67SL7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194475.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH341"/>
    <dbReference type="EnsemblBacteria" id="BAD39326">
        <property type="protein sequence ID" value="BAD39326"/>
        <property type="gene ID" value="STH341"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH341"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_8_9"/>
    <dbReference type="OMA" id="QFVALQW"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="16628" checksum="2BAB64424E6EE4B4" modified="2004-10-11" version="1">MLVKPSVSSQVAEIDSLLREVGGLLRKWGRDILGGFDITNPQFEALLVLREHGELTMGELCAKMFLACSTATDLIDRMERNQLIERVRDTADRRVIRLRVLPKGNHLIDQILEARQAYLSNAMAGMAASDKARLILSLQQLTAVLSREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM8</accession>
    <name>Q67SM8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194464.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH330"/>
    <dbReference type="EnsemblBacteria" id="BAD39315">
        <property type="protein sequence ID" value="BAD39315"/>
        <property type="gene ID" value="STH330"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH330"/>
    <dbReference type="HOGENOM" id="CLU_1814841_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="15005" checksum="08A7A60B6E02C3A3" modified="2004-10-11" version="1">MTVTLTQTQLLLAAVGLSVIVTLILGPIVTLLTRRVPASTVRTVLDGAEQVARVAEAILPDGTLPDKIARYAGMAVAAAEQMFDVSQREDKYAYADDLVRQALRAASVPEEQIERLGGVIQGAIEAAVLALPDSHKTEAVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN5</accession>
    <name>Q67SN5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH323"/>
    <dbReference type="EnsemblBacteria" id="BAD39308">
        <property type="protein sequence ID" value="BAD39308"/>
        <property type="gene ID" value="STH323"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH323"/>
    <dbReference type="HOGENOM" id="CLU_1408110_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="20185" checksum="F2215BC9C2D3459B" modified="2004-10-11" version="1">MEAVANVKAAGAEVVDAAKDQFAAMKEFGRDAAGVVRDFFKEKDKPPLPEVGDDAKYASDQLGDLSGTANTAAGGMDALRESAEKAGSAVREAFGGFKAYALGEVAYRLGALAPDWQSLAAQQYAAANRFSAGLPAAAYATAPANSTLTIDGSVRVEGVNSRGDLVAVTRLLADDLRAEAERYPAAPSQRRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ2</accession>
    <name>Q67SQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39291">
        <property type="protein sequence ID" value="BAD39291"/>
        <property type="gene ID" value="STH306"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH306"/>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="146"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="402" mass="45446" checksum="32BE1E4F43E8B38C" modified="2004-10-11" version="1">MAAVAGPDRSGMVIVDIDLTGQKVRGETRQYTGTDFGYIQGKLARGYQIAAAFLSGKQQRFAIDGLLKSGKANSRSGACLLELIPRIEARIGRPLRRVEWVEACLAQQKARVRQLYQQLQTVSGKGSARRKQKLQREFQEEVQHLREVNQRLRQYRQENRTNLAPLRILLRADSAFGTPEVIQRLLELGYEFTIKSYSGSNVAYKHLFDAVPAENWVEVEKNRFASEAVTVPGPTLLAPYPVRLVAMRRWDADGREVRSVILTTLQPEELTTTEVVKLYHGRQTIEAGFQEWKGTFHFGTPRLRKYEANAAFTQLVLFAFNLVRWAWRFLSTNSPKLAEAGSRLLVRVAARCRATIRCLGDTLRLVFSRGTPLAGAEITLNRATPYPYALLTPRMSSCSRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY5</accession>
    <name>Q67SY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-oxoglutarate oxidoreductase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194358.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH223"/>
    <dbReference type="EnsemblBacteria" id="BAD39208">
        <property type="protein sequence ID" value="BAD39208"/>
        <property type="gene ID" value="STH223"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH223"/>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_2_1_9"/>
    <dbReference type="OMA" id="LPTIFCP"/>
    <dbReference type="OrthoDB" id="981516at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="50"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="27211" checksum="42D1FF9D92874641" modified="2004-10-11" version="1">MKCIAGMPESLSPVVTHYCPGCSHGVLHRIIAELLDELGLRERTVGVASVGCSVLAYNYFETDFVQAPHGRAPAVATGVKRARPNLFVFTYQGDGDMAAIGTAEIIHAAARGERITAIMYNNTIYGMTGGQMSPTTLMGAVTATTPYGRQEESGRPYRMAELLAQMPGTAYAARTHVADVANIVKTKQYIRKAFEAQVNGLGFSMVEVLGNCPTNWGVTPAEANRYVLNDMLAYFPVGEFKDVTKEALAGVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T94</accession>
    <name>Q67T94_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pyruvate formate-lyase-activating enzyme</fullName>
            <ecNumber evidence="12">1.97.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH114</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glycyl-[formate C-acetyltransferase] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[formate C-acetyltransferase] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:19225"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12190"/>
            <dbReference type="Rhea" id="RHEA-COMP:12191"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
            <dbReference type="EC" id="1.97.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:61976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15993"/>
            <dbReference type="Rhea" id="RHEA-COMP:15994"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the organic radical-activating enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.97.1.4" evidence="12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH114"/>
    <dbReference type="EnsemblBacteria" id="BAD39099">
        <property type="protein sequence ID" value="BAD39099"/>
        <property type="gene ID" value="STH114"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH114"/>
    <dbReference type="eggNOG" id="COG1180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058969_1_1_9"/>
    <dbReference type="OMA" id="HIDEEMH"/>
    <dbReference type="OrthoDB" id="1141206at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043365">
        <property type="term" value="F:[formate-C-acetyltransferase]-activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012839">
        <property type="entry name" value="Organic_radical_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012838">
        <property type="entry name" value="PFL1_activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001989">
        <property type="entry name" value="Radical_activat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000371">
        <property type="entry name" value="PFL_act_enz"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02493">
        <property type="entry name" value="PFLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01087">
        <property type="entry name" value="RADICAL_ACTIVATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="28"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD39099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="27975" checksum="2EAAB44885086C59" modified="2004-10-11" version="1">MGRVHPEPTPDRARRTGRIHSVESCGAVDGPGLRFVVFVQGCPLRCRYCHNPDTWDPGQGAEVTVGSLIEEIQSYVPYMKFSGGGVTVSGGEPLLQPDFVADLFAECRRLFIHTALDTSGFAPPERARRVLEQTDLLLLDIKHPDLLRHKALTGVDGRPPRTTARLAAAMGIPIWIRYVVVPGWTDSPADVEALADLVQTLPTVEKVELLPYHLLGRHKWEALGLPYGLDGVAPPSEETLARIRQQLADRGIPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TB5</accession>
    <name>Q67TB5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LUD_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH93</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH93"/>
    <dbReference type="EnsemblBacteria" id="BAD39078">
        <property type="protein sequence ID" value="BAD39078"/>
        <property type="gene ID" value="STH93"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH93"/>
    <dbReference type="eggNOG" id="COG1556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090664_1_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LUD_dom" evidence="2">
        <location>
            <begin position="36"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="24616" checksum="559356EAC5ADD589" modified="2004-10-11" version="1">MAARLGRTPGEAPPRFRPSQPLPAVGPTEAAALADRFQLELEKISGVVHRVNDASEVAPTVVRILQAAQDAADEAARGSGPGPGASDPSRPGPPVARWDDPLLDGFGIDAALEEAGWPVIRFRAGDDGRAFASRVERAFAGVVACDAAIAETGTIVLHSGPTRGRTVSLLPPVLIAVLRKEQIVFDAARLFRRLARAPMPSQVIFKSGPSRSSDIENDLTIGIHGPGDLHVILL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC3</accession>
    <name>ATP6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpB</name>
        <name type="ordered locus">STH85</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194220.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TC3"/>
    <dbReference type="SMR" id="Q67TC3"/>
    <dbReference type="STRING" id="292459.STH85"/>
    <dbReference type="EnsemblBacteria" id="BAD39070">
        <property type="protein sequence ID" value="BAD39070"/>
        <property type="gene ID" value="STH85"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH85"/>
    <dbReference type="eggNOG" id="COG0356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041018_2_2_9"/>
    <dbReference type="OMA" id="ITPFIFP"/>
    <dbReference type="OrthoDB" id="867266at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045082">
        <property type="entry name" value="ATP_syn_F0_a_bact/chloroplast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42823">
        <property type="entry name" value="PTHR42823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000145340" description="ATP synthase subunit a">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <sequence length="257" mass="28040" checksum="46B89FD3A1A835B1" modified="2004-10-11" version="1">MTTFLFSLAEASGGKEFHIHQPFPVLLQGTYFEINNMIITQWLIVAGLLALAFVINRAVLSGRPSKLRGSLELLIDFLEGWFASFIGSRQYARQYMPLLCTLFLFIIVSNYLGVVPTVGYGLFAPTGRWGTTLGLGIVVAIAVQVIAIRTLGAGGWLKHILHLGPLSLLEEIVHPFSLSLRLFGNIFGEETLLAVIIFLAPMIAPIPIMVLSLVFGALQAVVFTTLTAIYIGAVLEHHQAHAHHPEGEAEHEPAPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE6</accession>
    <name>Q67TE6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH62</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194197.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH62"/>
    <dbReference type="EnsemblBacteria" id="BAD39047">
        <property type="protein sequence ID" value="BAD39047"/>
        <property type="gene ID" value="STH62"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH62"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_260492_0_0_9"/>
    <dbReference type="OMA" id="WVTATDP"/>
    <dbReference type="OrthoDB" id="66120at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269977" evidence="7">
        <location>
            <begin position="35"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="8">
        <location>
            <begin position="132"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1102" mass="117265" checksum="B5A04B0677A88CE2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH6</accession>
    <name>Q67TH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH32</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH32"/>
    <dbReference type="EnsemblBacteria" id="BAD39017">
        <property type="protein sequence ID" value="BAD39017"/>
        <property type="gene ID" value="STH32"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH32"/>
    <dbReference type="HOGENOM" id="CLU_3158618_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="48" mass="5050" checksum="B99BD87B561D37E8" modified="2004-10-11" version="1">MGIAVLATSFLLLVSARQDFHRLSPEPREPGQLAAQLVSLFLEGAAAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1993-07-01" modified="2022-05-25" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>P31015</accession>
    <accession>Q67SB9</accession>
    <accession>Q9R5M8</accession>
    <name>TNAA2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Tryptophanase 2</fullName>
            <ecNumber>4.1.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>L-tryptophan indole-lyase 2</fullName>
            <shortName>TNase 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tnaA2</name>
        <name type="synonym">tna-2</name>
        <name type="ordered locus">STH439</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1992" name="Appl. Environ. Microbiol." volume="58" first="2633" last="2642">
            <title>Cloning, nucleotide sequences, and overexpression in Escherichia coli of tandem copies of a tryptophanase gene in an obligately symbiotic thermophile, Symbiobacterium thermophilum.</title>
            <authorList>
                <person name="Hirahara T."/>
                <person name="Suzuki S."/>
                <person name="Horinouchi S."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="1339259"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 2-35</scope>
        <source>
            <strain>IAM 13621</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Agric. Biol. Chem." volume="55" first="3059" last="3066">
            <title>Purification and properties of thermostable tryptophanase from an obligately symbiotic thermophile, Symbiobacterium thermophilum.</title>
            <authorList>
                <person name="Suzuki S."/>
                <person name="Hirahara T."/>
                <person name="Horinouchi S."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="1368766"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-36</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + L-tryptophan = indole + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19553"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16881"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="EC" id="4.1.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid degradation; L-tryptophan degradation via pyruvate pathway; indole and pyruvate from L-tryptophan: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text>Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-eliminating lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Tna2 is expressed in strain T but expression in strain IAM 13621 has not been proven.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.1"/>
    <dbReference type="EMBL" id="AB010832">
        <property type="protein sequence ID" value="BAA24687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B49022">
        <property type="entry name" value="B49022"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194573.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P31015"/>
    <dbReference type="SMR" id="P31015"/>
    <dbReference type="STRING" id="292459.STH439"/>
    <dbReference type="EnsemblBacteria" id="BAD39424">
        <property type="protein sequence ID" value="BAD39424"/>
        <property type="gene ID" value="STH439"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH439"/>
    <dbReference type="eggNOG" id="COG3033">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047223_0_0_9"/>
    <dbReference type="OMA" id="MFPHIPY"/>
    <dbReference type="OrthoDB" id="91973at2"/>
    <dbReference type="BRENDA" id="4.1.99.1">
        <property type="organism ID" value="6177"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00332">
        <property type="reaction ID" value="UER00452"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009034">
        <property type="term" value="F:tryptophanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00544">
        <property type="entry name" value="Tryptophanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011166">
        <property type="entry name" value="Beta-eliminating_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013440">
        <property type="entry name" value="TNase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018176">
        <property type="entry name" value="Tryptophanase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001386">
        <property type="entry name" value="Trpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00853">
        <property type="entry name" value="BETA_ELIM_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2 3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000195623" description="Tryptophanase 2">
        <location>
            <begin position="2"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; BAA24687." evidence="4" ref="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1339259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="1368766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="454" mass="50661" checksum="5EB609F6EFE866CB" modified="2007-01-23" version="4">MPKGEPFKIKMVEPIRLIPREDREAAIKAAHYNPFLLRSSDVYIDLLTDSGTGAMSQFQWSAMMLGDESYAGASSYYRLKEAVTDITGYEYVLPTHQGRGAEKSAFAQLITRPGMYVLSNMFFDTTRGHVQLAGGRPIDLLLDVPTEEYHPFKGNMDTARLEAFIQEHGAENIACIVMTVTNNSAGGQPVSMANIRETSRIARKYGILLLFDVARYAENCHFIRMREEGYADKAPIDIAREMFSYGDGLMMSAKKDALVNIGGLLAFKDEELYTRVGGTVVPFEGFLTYGGLAGRDLEAMAVGLREALDPDYLAYRVGQVEYLGNLLRSAGIPIQWPVGGHAVFIDAAKFLPHIPWDQFPGHALTVALYQEGGVRTVEVGSLVMGRDPETGENVRSPFEFTRLAIPRRVYTNLHLEDVAETVINAFQKREQIRGVKFTREPKVLRHFTAHFDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA3</accession>
    <name>Q67JA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197378.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3266"/>
    <dbReference type="EnsemblBacteria" id="BAD42247">
        <property type="protein sequence ID" value="BAD42247"/>
        <property type="gene ID" value="STH3266"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3266"/>
    <dbReference type="eggNOG" id="COG3773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053345_3_1_9"/>
    <dbReference type="OMA" id="YIELTEM"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011105">
        <property type="entry name" value="Cell_wall_hydrolase_SleB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042047">
        <property type="entry name" value="SleB_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07486">
        <property type="entry name" value="Hydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_2" evidence="1">
        <location>
            <begin position="56"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="155" mass="17067" checksum="9E633266733EF23A" modified="2004-10-11" version="1">MAVHRWLKWTAALLAAGALLLWPEATFDGHRTFAESRAAKDNLHLLAQVVCGEARGEPYEGKVAVAAVILNRTLDNRFPDSVAGVVYQTHAFESVSNGEIYKETTEECFKAARAALAGWDPSNGALYFFAPAKTSNAFIWSREQVRTIGKHIFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF2</accession>
    <name>Q67JF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3216"/>
    <dbReference type="EnsemblBacteria" id="BAD42198">
        <property type="protein sequence ID" value="BAD42198"/>
        <property type="gene ID" value="STH3216"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3216"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109730_1_0_9"/>
    <dbReference type="OMA" id="IGLVRHF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="22460" checksum="EF448504A955A75E" modified="2004-10-11" version="1">MRVQWKHVREAVNSTVKIGLVRHFRVKHAYPAKLLVTPDEVAEWFAGYDNADIEYGHVDLSGGDWQHCFCSDLPRAVKTARAIFPGEIIILKDLREIEPYPFRGNRIKLPFLVWAVLVRLVWYFKSHPNVESRRAVRERVRRVVDRVLQSDSDALVVSHAALMPFLRSELKRRGFRGPWFGHAANGLLYVFER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH9</accession>
    <name>FTHS_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="1">6.3.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formyltetrahydrofolate synthetase</fullName>
            <shortName evidence="1">FHS</shortName>
            <shortName evidence="1">FTHFS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fhs</name>
        <name type="ordered locus">STH3189</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197302.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JH9"/>
    <dbReference type="SMR" id="Q67JH9"/>
    <dbReference type="STRING" id="292459.STH3189"/>
    <dbReference type="EnsemblBacteria" id="BAD42171">
        <property type="protein sequence ID" value="BAD42171"/>
        <property type="gene ID" value="STH3189"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3189"/>
    <dbReference type="eggNOG" id="COG2759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003601_3_3_9"/>
    <dbReference type="OMA" id="TRQGFSK"/>
    <dbReference type="OrthoDB" id="177859at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000199401" description="Formate--tetrahydrofolate ligase">
        <location>
            <begin position="1"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01543"/>
        </source>
    </evidence>
    <sequence length="556" mass="59450" checksum="49822EE6AAD9BDAE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK3</accession>
    <name>Q67JK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3165"/>
    <dbReference type="EnsemblBacteria" id="BAD42147">
        <property type="protein sequence ID" value="BAD42147"/>
        <property type="gene ID" value="STH3165"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3165"/>
    <dbReference type="eggNOG" id="COG5482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075295_0_0_9"/>
    <dbReference type="OMA" id="IMTAYRQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018679">
        <property type="entry name" value="DUF2161"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09929">
        <property type="entry name" value="DUF2161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="220" mass="24443" checksum="1788C27019DFE8A4" modified="2004-10-11" version="1">MKSLLEGLGFTVRGEVNGCDLAAVRGDDLVIVELKRTVNLDLLLQAVDRLRLTDLVYVAVEAPRRARGRRWTQIQHLCRRLGLGLMVVRFTGGGPAAAVLFDPEPWRPRPRSRQRAALLREVARRSGDYNVGGSTRSPLITAYREEALRIAAYLREQGPSAVRTIRAATGVEAAAPILQRNYYGWYERTARGVYRLTPAGEEALRTYAHVVSRLGGRPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM2</accession>
    <name>Q67JM2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Plastoquinol--plastocyanin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3146</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3146"/>
    <dbReference type="EnsemblBacteria" id="BAD42128">
        <property type="protein sequence ID" value="BAD42128"/>
        <property type="gene ID" value="STH3146"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3146"/>
    <dbReference type="eggNOG" id="COG0723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="PTHR10134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="9">
        <location>
            <begin position="67"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="178" mass="19045" checksum="D5E4432A8B209C70" modified="2004-10-11" version="1">MAAENQVHAAAAAVPPAHGPAADGMTRRQLLRSTMFATIGAMFGLAGAGAGGMLWPIKLTGFGGIVPAPVKAHEMEIGDVVMVREGKYYLTRSEDGLMALYWKCVHLGCTVPWNAAQKKFMCPCHASVYEITGQNIAGPAPRPLDMMEVIVEPDGTVLVNTGKITERPRHEPEHAVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN2</accession>
    <name>Q67JN2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcriptional regulator CtsR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH3136</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the CtsR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197249.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3136"/>
    <dbReference type="EnsemblBacteria" id="BAD42118">
        <property type="protein sequence ID" value="BAD42118"/>
        <property type="gene ID" value="STH3136"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3136"/>
    <dbReference type="eggNOG" id="COG4463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118139_0_0_9"/>
    <dbReference type="OMA" id="FRCAPSQ"/>
    <dbReference type="OrthoDB" id="2005536at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008463">
        <property type="entry name" value="CtsR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041473">
        <property type="entry name" value="CtsR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041908">
        <property type="entry name" value="CtsR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040465">
        <property type="entry name" value="CtsR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041902">
        <property type="entry name" value="CtsR_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40028">
        <property type="entry name" value="PTHR40028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05848">
        <property type="entry name" value="CtsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17727">
        <property type="entry name" value="CtsR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010607">
        <property type="entry name" value="Txn_repr_CtsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CtsR" evidence="8">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CtsR_C" evidence="9">
        <location>
            <begin position="76"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD42118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="17311" checksum="C5DE4F2E61DCB9F1" modified="2004-10-11" version="1">MANLADKIERYLKEHLSRTEILEVRRAMLAALFDCAPSQINYVLTTRFTPERGYLVESRRGGGGYIRIIRLNIKNPEDLQDLIERQIGDAISQNEAFHIVGRLRDEGLIDEREAAIMEAAVRREVLGLELPYRDEVRASVLKAMLTAILRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR8</accession>
    <name>Q67JR8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Formate dehydrogenase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 4 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3100"/>
    <dbReference type="EnsemblBacteria" id="BAD42082">
        <property type="protein sequence ID" value="BAD42082"/>
        <property type="gene ID" value="STH3100"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3100"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_0_3_9"/>
    <dbReference type="OMA" id="KACQSAC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015944">
        <property type="term" value="P:formate oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014603">
        <property type="entry name" value="Formate_DH_Fe-S_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036298">
        <property type="entry name" value="FDH_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
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            <begin position="253"/>
            <end position="271"/>
        </location>
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            <begin position="21"/>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
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        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="36"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="40"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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            <name>[4Fe-4S] cluster</name>
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            <name>[4Fe-4S] cluster</name>
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        <location>
            <position position="156"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        </ligand>
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        <location>
            <position position="159"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
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        <location>
            <position position="171"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
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        <location>
            <position position="175"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036298-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="291" mass="32231" checksum="7B7E412D6173FAA6" modified="2004-10-11" version="1">MGRAATWETWRPDPANTRQHVAMLVDTSECIGCKGCEVACKQWNQNEARINQDPGTYQSHPALDAETWTVIRFFETEEENGMSMWLMQKHSCMHCTDAGCVTACPTDALQYGDYGLVTLNQDACIGCGYCEAACPFDCIHVDRTAWGQRAQKAGKCTFCYDRIAQGMQPACVATCPTDCIKYGDRDALIAWGRERVAQLQAMGYKNANLYGVDEMGGLHHLYVLLEPPETYGLPVAPELSPVLRLWKYVLQPIGKAVLGVGLFGLIVNWLAARRVFKGGLSPHETAESLHD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV5</accession>
    <name>RL5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplE</name>
        <name type="ordered locus">STH3063</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197178.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV5"/>
    <dbReference type="SMR" id="Q67JV5"/>
    <dbReference type="STRING" id="292459.STH3063"/>
    <dbReference type="EnsemblBacteria" id="BAD42045">
        <property type="protein sequence ID" value="BAD42045"/>
        <property type="gene ID" value="STH3063"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3063"/>
    <dbReference type="eggNOG" id="COG0094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_2_1_9"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="1456375at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000243073" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="180" mass="20072" checksum="CD340192F61EDE38" modified="2004-10-11" version="1">MANLKTKYQQEVAPALQKQFNYKNVMMIPRLEKIVINVGLGEAVQNAKALDAAVADIAAIAGQRPVITRAKKSISSFKIRTGMPIGCKVTLRGERMWDFLEKLIYVALPRVRDFRGVSPKSFDGRGNYALGLKEQLLFPEIDYDKVDKIRGMDVIIVTTAKTDEEAKALLKGLGMPFAER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW7</accession>
    <name>RL36_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmJ</name>
        <name type="ordered locus">STH3051</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197166.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW7"/>
    <dbReference type="SMR" id="Q67JW7"/>
    <dbReference type="STRING" id="292459.STH3051"/>
    <dbReference type="EnsemblBacteria" id="BAD42033">
        <property type="protein sequence ID" value="BAD42033"/>
        <property type="gene ID" value="STH3051"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3051"/>
    <dbReference type="eggNOG" id="COG0257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135723_6_2_9"/>
    <dbReference type="OrthoDB" id="2066169at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42888">
        <property type="entry name" value="PTHR42888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000126277" description="50S ribosomal protein L36">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <sequence length="37" mass="4295" checksum="C2EBC462C6BF4CF4" modified="2004-10-11" version="1">MKVRPSVKPICEKCKVIKRHGHVMVICENPKHKQKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX7</accession>
    <name>Q67JX7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsI</name>
        <name evidence="9" type="ordered locus">STH3040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3040"/>
    <dbReference type="EnsemblBacteria" id="BAD42023">
        <property type="protein sequence ID" value="BAD42023"/>
        <property type="gene ID" value="STH3040"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3040"/>
    <dbReference type="eggNOG" id="COG0103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_2_1_9"/>
    <dbReference type="OMA" id="QWKINGR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="128" mass="14379" checksum="21AE95696850871C" modified="2004-10-11" version="1">MTQYWGTGRRKTAIARARLLPGTGNFVVNGRTLQEYFGLQTLQMMVESPFTVTGTTGKFDVYLNVIGGGISGQAGACRHAIARALVKYDPELRPALKQAGFLTRDPRMKERRKYGLKKARKRPQFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ3</accession>
    <name>Q67JZ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197139.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3024"/>
    <dbReference type="EnsemblBacteria" id="BAD42007">
        <property type="protein sequence ID" value="BAD42007"/>
        <property type="gene ID" value="STH3024"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3024"/>
    <dbReference type="eggNOG" id="COG2984">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058196_1_0_9"/>
    <dbReference type="OMA" id="NMDSETP"/>
    <dbReference type="OrthoDB" id="1729219at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06325">
        <property type="entry name" value="PBP1_ABC_unchar_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007487">
        <property type="entry name" value="ABC_transpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35271">
        <property type="entry name" value="PTHR35271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04392">
        <property type="entry name" value="ABC_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269379" evidence="2">
        <location>
            <begin position="23"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="337" mass="35245" checksum="97C62909404395A6" modified="2004-10-11" version="1">MRRVLLACLAAALVLAGCGGSASQPQGTSGSDPSSGSDGRVRIGLTQIVEHPALDATRQGIIDGLKDAGYEDGKQIEIDFQSAQGEQPVAQQIAEKFVADKKDLIIAIATPSAQAVVQATRSNPIPVVFSAVTDPLSAGLVSDLQKPGGHVTGTSDLVPVKIQMELFDRLGLNVTNVGVIYNAGEANSVALMKDIKVAAEELGLNIVEATVANATEVQQAAQSLVGRVDGFYLITDNTLAQGVQAVIDIANTHKIPAISSVDSYVEQGALATFGLDYYLHGRLTAEVVAEILQGKDPGDLPVRYNEDLSLIINTRAVETLGLDIPAAVLEEARKVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K13</accession>
    <name>Q67K13_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar hook-basal body complex protein FliE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">fliE</name>
        <name evidence="4" type="ordered locus">STH3004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the FliE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3004"/>
    <dbReference type="EnsemblBacteria" id="BAD41987">
        <property type="protein sequence ID" value="BAD41987"/>
        <property type="gene ID" value="STH3004"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3004"/>
    <dbReference type="eggNOG" id="COG1677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147249_3_0_9"/>
    <dbReference type="OMA" id="LHDVMIT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00724">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001624">
        <property type="entry name" value="FliE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34653">
        <property type="entry name" value="PTHR34653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02049">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01006">
        <property type="entry name" value="FLGHOOKFLIE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00205">
        <property type="entry name" value="fliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="11190" checksum="FEA999CA851C728D" modified="2004-10-11" version="1">MDIQRINAMLSLEPTISRENRSEVREGTSFGDVLANAIRQVEQDQRHANEMAVRLAAGEVEDIAQVMIASERASLSLNLAIQVRNKVIEAYQEIMRMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K37</accession>
    <name>Q67K37_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flaG</name>
        <name evidence="2" type="ordered locus">STH2978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2978"/>
    <dbReference type="EnsemblBacteria" id="BAD41961">
        <property type="protein sequence ID" value="BAD41961"/>
        <property type="gene ID" value="STH2978"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2978"/>
    <dbReference type="eggNOG" id="COG1334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120910_3_1_9"/>
    <dbReference type="OMA" id="HKESKRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005186">
        <property type="entry name" value="FlaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035924">
        <property type="entry name" value="FlaG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37166">
        <property type="entry name" value="PTHR37166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03646">
        <property type="entry name" value="FlaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160214">
        <property type="entry name" value="SSF160214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="12612" checksum="B8F5CEDF70EBC046" modified="2004-10-11" version="1">MTAPVSGPTPVPDRTVTPRETADLGKLQTQEEQRKQSQPLSKEQAEELAEKMNSAAELFNKAVQFKVVEGNRIVIKVVDVTTDEVLREFPPERLLEALLAMERSLGLLIDEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K76</accession>
    <name>Q67K76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2939"/>
    <dbReference type="EnsemblBacteria" id="BAD41922">
        <property type="protein sequence ID" value="BAD41922"/>
        <property type="gene ID" value="STH2939"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2939"/>
    <dbReference type="HOGENOM" id="CLU_2439683_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="90" mass="9638" checksum="D58C34D77BECFD28" modified="2004-10-11" version="1">MEKPTGKLCSAGGVAVRSRLFAPGRRLVAEVVSVYLDRAVLAFGDGVRIEVAARAPLREGERVILRVERARNGAVVLRLAGDEAGLDRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB3</accession>
    <name>Q67KB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2902</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197019.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2902"/>
    <dbReference type="EnsemblBacteria" id="BAD41885">
        <property type="protein sequence ID" value="BAD41885"/>
        <property type="gene ID" value="STH2902"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2902"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_550850_0_0_9"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="97"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="495" mass="54182" checksum="6896CDE17525AFAD" modified="2004-10-11" version="1">MDYFQLLRAHQGYLERIALAMLGNRPDAEDALQEAALAGYRRFAQLRDEQAFGAWMRRILIRQCRQILERRRRSFPVDDLAAHLPDTVPGPDPEATAIWEMVARLGDHLRPVVVLRYMLDMSQQEIAEALGIPLGTVKSRLGKALPLVGPVITRFILAGAGLQWAYDMGLIGEPVAEVTQNGITVRILGVVADARRTTVLYQVLGAPSTVGRGRQPRVPVHATITAQMSEFTPPEETPLGYLGTVSTLPLEAEAAELEVRFDVGAETITLTVPASRTETDRFSREVAVNRALEYDGVRITVEAVIYTPAETIVRYREEVTESFDFPVQWDGDVHCNYLEAGGQRYRQSGSGGGLNSVSYLAFPPVRGPARLVLKPAVKAEPLDAVWPLEEGAVAEVVGVPVTLAGIERRPGAIELSWTSPVREGFVGVSGFEVIDGEGNVYPLGGSWAGFSNYTHDGVRYQRYETELPEGLEPVAVRATKAGVLVDGPWVFDLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF5</accession>
    <name>Q67KF5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2858"/>
    <dbReference type="EnsemblBacteria" id="BAD41843">
        <property type="protein sequence ID" value="BAD41843"/>
        <property type="gene ID" value="STH2858"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2858"/>
    <dbReference type="eggNOG" id="COG0152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1502742_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="19826" checksum="F87DC70677613D54" modified="2004-10-11" version="1">MEEPVLKRERLWAGDGLAVHATDRADRLILEFEDETVCAAALRIQEILVGHGLATSFAARFTSRSFLIRKVQPLPVEVAAEAGPGEVRLAFRDGDRELSREEAEAALTPERLERIEDAALHAARTLRAHLSLRGVERLQLRMRFGLTEEGACLMQVMNPLECRLGSEDMKEVLALLGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF9</accession>
    <name>Q67KF9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="5">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purF</name>
        <name evidence="11" type="ordered locus">STH2854</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5 9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2854"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="BAD41839">
        <property type="protein sequence ID" value="BAD41839"/>
        <property type="gene ID" value="STH2854"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2854"/>
    <dbReference type="eggNOG" id="COG0034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_9"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="32"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 7">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 9">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="495" mass="52876" checksum="4EE8FE5BC85CAC40" modified="2004-10-11" version="1">MIRPGDPTLALALQPYLGGWPTNPDKGPADECGVFGIYGHPEAARVVYHALIALQHRGQESAGIVAADGANLNTHRGMGLVSDVFEKPETIQRLAGDIAIGHVRYSTTGSSRLGNAQPVVVNTRRGGLALAHNGNLVDAPAIRDRLEEQGAIFTTSIDTEVLAHLIVRSRAKSLEDAIVDAVSQVHGGYALLILAEDRLIGIRDPHGIRPLQLGRLDGSWVLASETCAFDTIGAEFVREVAPGEMVTISEGGKLRSRAAVREAVAPRPCIFEFIYFARPDSQFVGVNVHTVRKAMGRQLAKEAPADADIVIGVPDSSISAATGYAEESGIPYEVGLVKNRYIARTFILPSQAGRESALKLKLNPLRKVIEGRRVVLVDDSIVRGTTSRHLVSLLREAGAREVHLRIASPPYQNACHYGIDTSKSTDLIARGRTVREIADAIGADSLAYLSVEGMVKATGLSPEAGFCLACFTGDYPVPVPEEADKYALEGGCGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK1</accession>
    <name>Q67KK1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exodeoxyribonuclease-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196931.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2812"/>
    <dbReference type="EnsemblBacteria" id="BAD41797">
        <property type="protein sequence ID" value="BAD41797"/>
        <property type="gene ID" value="STH2812"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2812"/>
    <dbReference type="eggNOG" id="COG0507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006651_0_0_9"/>
    <dbReference type="OrthoDB" id="961809at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="589"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1196" mass="138217" checksum="1A654E495FBC7AAD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV5</accession>
    <name>Q67KV5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2706</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2706"/>
    <dbReference type="EnsemblBacteria" id="BAD41691">
        <property type="protein sequence ID" value="BAD41691"/>
        <property type="gene ID" value="STH2706"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2706"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_12_2_9"/>
    <dbReference type="OMA" id="ASFFIHS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="7">
        <location>
            <begin position="56"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="6">
        <location>
            <begin position="277"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="228"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="356"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="452" mass="52201" checksum="2C48C470A4F04044" modified="2004-10-11" version="1">MSKKTYRPYEPNQLLLLPPALQDWLPHDHFAYFLSDVVDSLDLSQIERTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMSYSRMVEEEQRLKAEIAELLQKAEQADQAEDHRFGPHRRGDELPEELRFRERRLAKIQEAKAALEAEARLRAGADEHEPPEEPGPKGPKGGRRRKRPKAEPAPTAQRNFTDPDSRIMKGSDKQFIQGYNAQAVVDADTQIVVAAAVTNQAADAPHLIPMMEQVETNVDEMPEEVSADAGYFSEQNVQWLLQRRINPYIPPEKQRHSERRRAKSPRGPIPKGATLRDRMRRKLQLKRHAERYRKREQSIEPVFGQIKEARGFRQFHLRGLEAVNGEWLLVCLCHNLLKLYAFRQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX5</accession>
    <name>Q67KX5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="15">BCAT</shortName>
            <ecNumber evidence="15">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvE</name>
        <name evidence="16" type="ordered locus">STH2686</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 15">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 15">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 15">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 13">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="15"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196808.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2686"/>
    <dbReference type="EnsemblBacteria" id="BAD41671">
        <property type="protein sequence ID" value="BAD41671"/>
        <property type="gene ID" value="STH2686"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2686"/>
    <dbReference type="eggNOG" id="COG0115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_9"/>
    <dbReference type="OMA" id="WRGSEMM"/>
    <dbReference type="OrthoDB" id="1275805at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="12 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 16" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD41671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="291" mass="32548" checksum="65D22DE898CD7D72" modified="2004-10-11" version="1">MSIQVYVDGEWYSRDEAKVSVFDHGFLYGDGVFEGIRAYGGRVFRLTEHLDRLYDSARAIWLEIPLSWREMEGVVLETLRRSNLRDAYIRLVVSRGRGDLGLDPRKCPRPTVVCIADSIALYPEEVYTRGMQVISVSTRRNPPDTVWPQVKSLNYLTSIIAKISAATQGYPEVIMLNKEGYVCEGTGDNIFIVKKGRLITPAVHLGILPGITRAAVMEYARDLGYAVEEGTFTLYDLYTADECFLTGTAAEIVPVVECDARKIGSGQPGEITRQLIAEFRRRVQVEGTPIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ3</accession>
    <name>GLUQ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-Q tRNA(Asp) synthetase</fullName>
            <shortName evidence="1">Glu-Q-RSs</shortName>
            <ecNumber evidence="1">6.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gluQ</name>
        <name type="ordered locus">STH2668</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196790.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KZ3"/>
    <dbReference type="SMR" id="Q67KZ3"/>
    <dbReference type="STRING" id="292459.STH2668"/>
    <dbReference type="EnsemblBacteria" id="BAD41653">
        <property type="protein sequence ID" value="BAD41653"/>
        <property type="gene ID" value="STH2668"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2668"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_0_0_9"/>
    <dbReference type="OMA" id="WLLRMED"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01428">
        <property type="entry name" value="Glu_Q_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022380">
        <property type="entry name" value="Glu-Q_tRNA(Asp)_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03838">
        <property type="entry name" value="queuosine_YadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000208328" description="Glutamyl-Q tRNA(Asp) synthetase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="249"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01428"/>
        </source>
    </evidence>
    <sequence length="323" mass="34743" checksum="CDB3D7538A8CF87C" modified="2004-10-11" version="1">MLCGRFAPTPSGALHLGNARTALLAWLHARRAGGRFILRIEDIDRARSRPHLAEQAIADLRWLGLDWDEGPDVGGPHGPYCQSEREELYRDALARLQAEGRLYPCYCSRAQLMAIASAPHGLTSEGPAYPGTCRRLTPEERRAREADGKTPSLRFALPDEEIAFTDLIAGPQRFPPGAGGDFVVLRADGVIGYQLAVVVDDALMGVTHVLRGGDLLDSTPRQILLYRALGRPVPAFGHLPLLLGPDGARLAKRHGAVTLAGIRAAGTSPETVVGHLAYLSGLVDQPEPVRPEELIPVFDLARIPREPVRIPAETIAALSGGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L15</accession>
    <name>Q67L15_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196768.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2646"/>
    <dbReference type="EnsemblBacteria" id="BAD41631">
        <property type="protein sequence ID" value="BAD41631"/>
        <property type="gene ID" value="STH2646"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2646"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1037990_0_0_9"/>
    <dbReference type="OrthoDB" id="1389691at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="121"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="268" mass="29856" checksum="7690A0192974058B" modified="2004-10-11" version="1">MDVALVAERWEENQEHLKQHLGESTSQLFGARWVLMPSAPYPRYNHVGCIRVDGDQVEAMIAAARAFFWEHGLPHVAFLITPATRPADLADRLLREGFRTETVPVMVWDGTPLPAPRSPDLRVVRMLPGQWELFWQVMRQVFFPGADGDYLAAGRKGVEVADALGARHYVAFLGQQPVGGGTLYPHARMGGIYNLCTLPAYQRRGVATAVIRTCIADALEMGCEHVALTPTAMGRRLYLRLGFRDAYVERYLFQRIGPLVPEAATGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L30</accession>
    <name>Q67L30_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2631</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196753.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2631"/>
    <dbReference type="EnsemblBacteria" id="BAD41616">
        <property type="protein sequence ID" value="BAD41616"/>
        <property type="gene ID" value="STH2631"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2631"/>
    <dbReference type="eggNOG" id="COG0490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112362_0_0_9"/>
    <dbReference type="OMA" id="INFWHNT"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="121"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="23491" checksum="45ED317E4CB8B3E5" modified="2004-10-11" version="1">MKREQTGLPRYEAIALDLARRIACGELEEGSRLLGRSSLAGTYQVSPETIRRAVAILHERGVVKAVAGSGIRILSQVAAQEYVEAVGSQFRVEQEIRELRQLLRERRRLDEEIENALDRLLKHTSGAIGTRHVEELVLDSHSWAVGRSLGEIRLRSSTGVTAVAITRGNEEYFSPPADMELKAGDVLTIVGSDAARARCRELLAATVVPEREP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L43</accession>
    <name>CTAA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Heme A synthase</fullName>
            <shortName evidence="1">HAS</shortName>
            <ecNumber evidence="1">1.17.99.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytochrome aa3-controlling protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaA</name>
        <name type="ordered locus">STH2618</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 A + Fe(II)-heme o + H2O = 2 AH2 + Fe(II)-heme a</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with CtaB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COX15/CtaA family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67L43"/>
    <dbReference type="SMR" id="Q67L43"/>
    <dbReference type="STRING" id="292459.STH2618"/>
    <dbReference type="EnsemblBacteria" id="BAD41603">
        <property type="protein sequence ID" value="BAD41603"/>
        <property type="gene ID" value="STH2618"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2618"/>
    <dbReference type="eggNOG" id="COG1612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041525_3_0_9"/>
    <dbReference type="OMA" id="YTGAYVR"/>
    <dbReference type="UniPathway" id="UPA00269">
        <property type="reaction ID" value="UER00713"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01664">
        <property type="entry name" value="HemeA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023755">
        <property type="entry name" value="HemeA_Synthase_type1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000349005" description="Heme A synthase">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01664"/>
        </source>
    </evidence>
    <sequence length="306" mass="32848" checksum="D8DAF5A1D807FAB5" modified="2004-10-11" version="1">MKALRAVSLANTAVMLLAVLWGAWVTSSDSGDGCGASWPLCKGTFMPDWDYAAIVEFGHRVVSALAGLLSVAVLVWVARVRPSETRLKRLAFGTFFFVVLQGGLGAAAVLRPQPDLVMALHFGFSLLCFTFALLVTVALGQGERAAFQRPDVSAQPVAPGLRTQIWGLAVYTYLVVYLGAYVRHLGASMACTGWPLCNGELIPPLYGPVGANFAHRLGAALAVVLVLRLWWTARRLTERDDLRRGAAWALALMAAQVASGALFPLGYLNLLTQLLHTGLITGFWGVLSYLCYLTLPVGRETVAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L77</accession>
    <name>Q67L77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxybutyryl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2584</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196706.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2584"/>
    <dbReference type="EnsemblBacteria" id="BAD41569">
        <property type="protein sequence ID" value="BAD41569"/>
        <property type="gene ID" value="STH2584"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2584"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_9"/>
    <dbReference type="OMA" id="FIREVTH"/>
    <dbReference type="OrthoDB" id="862072at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="3">
        <location>
            <begin position="7"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="2">
        <location>
            <begin position="186"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="32831" checksum="302B2DC9707AA2E0" modified="2004-10-11" version="1">MSVRIWGIVGTGPSGRGIAQLVATQGLEVIMVGRSEEELEQARRQLDLALQHEIEKWALTQSEKRAILARISMTTDINELAKADFVIATLVVEIAEDKEIFRTLDQVCRREVILASNTSTLSITEMASATNRPDKVIGCHFLQPIPRTRVVQVVRGLKTSDETVSQVMALMERLGRTGVEVFESPGYITTRLIVPLINEACQVLMEGVASAEDIDTAMRLGFEMPRGPLEIADRIGLDTVLVMAERLWREYGDLKYRPAPILKKLVRAGHLGVETGEGFFKYDADGARIKNGRTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L78</accession>
    <name>Q67L78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196705.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2583"/>
    <dbReference type="EnsemblBacteria" id="BAD41568">
        <property type="protein sequence ID" value="BAD41568"/>
        <property type="gene ID" value="STH2583"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2583"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036903_0_0_9"/>
    <dbReference type="OMA" id="HKQIFFV"/>
    <dbReference type="OrthoDB" id="231306at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024300">
        <property type="entry name" value="DUF3794"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12673">
        <property type="entry name" value="DUF3794"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3794" evidence="1">
        <location>
            <begin position="36"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3794" evidence="1">
        <location>
            <begin position="176"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3794" evidence="1">
        <location>
            <begin position="312"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="445" mass="49251" checksum="2611A1A7BB493D52" modified="2004-10-11" version="1">MADLLSTQLLKLQCLVCENTGEACIESRFKLPRKAKKIAEIQARIVDLEADVIDDQVCISGVVHKQIFFVGEDHHVHHVPEDVPFTTFVNCPGAKAGDIAEVKANIAKVNFNLEWGQEVVQRVIVQFVVRVSEDCQVNVRLNPRGPLVKAECVVGEATKAVTIENCIELDRRAIKIRDLQVSLEDVKAEATSDQVMFQGTLVKNIFYISDEDVELFQEERIPFSGIADVCGAEPGDNVTLQAQIVRVDKVLSDGVELRQRVVLSLFIKVTRTCEINVAEDPTGPQVMASRVIATGERQVLVENVTDLNKPAQKIQEIQARVEDVAVEVIPNKVIITGTLHKQIFFVGEDDIVRHQGEDIPFTTFVDLPGVNPGDEISVTPVVEHVGFDLLDKVWVSHHGDDCDDDEGRPVFRRLLQRTVILLCVTGSQEHPIRIAQAPFTGLAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L89</accession>
    <name>Q67L89_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2572"/>
    <dbReference type="EnsemblBacteria" id="BAD41557">
        <property type="protein sequence ID" value="BAD41557"/>
        <property type="gene ID" value="STH2572"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2572"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1712345_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="16291" checksum="117387001EE74A1E" modified="2004-10-11" version="1">MQAGSWTWRDDNGICGIRPAGRAPHEGSPAPEPGALPVPARTGTGVAPGGDRAGHGRLPGGDQSACRDPGQADVLAQHFTGVHPDHRGLGIALALKCLVTRYAQQNGYRRVTTATQETNAAMLAVNRRLGFRVAYSHLRLERFPGHSRRTADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L92</accession>
    <name>Q67L92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2569"/>
    <dbReference type="EnsemblBacteria" id="BAD41554">
        <property type="protein sequence ID" value="BAD41554"/>
        <property type="gene ID" value="STH2569"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2569"/>
    <dbReference type="eggNOG" id="COG4031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1577701_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018664">
        <property type="entry name" value="DUF2103_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09876">
        <property type="entry name" value="DUF2103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="19015" checksum="4829B4729E2BF331" modified="2004-10-11" version="1">MEATRPLYPRTETSLKKYRAGKIKREHSVIEHLWPVLEQIAVCEHVTSIIPGPIRPLGTQGFEVTFQRFTETGLRLLAKNGTAVQEVYIVAPDRQAALEWLAAEGIVEWKPKREGEPARRRDAPEPLQTVTLPSDMPCAVCGRAMRAGTRAAVLGRKRHVYAHVRCARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB3</accession>
    <name>Q67LB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196671.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2548"/>
    <dbReference type="EnsemblBacteria" id="BAD41533">
        <property type="protein sequence ID" value="BAD41533"/>
        <property type="gene ID" value="STH2548"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2548"/>
    <dbReference type="HOGENOM" id="CLU_2588426_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="80" mass="8222" checksum="64747D61A22779D0" modified="2004-10-11" version="1">MNFGGFGTDIATFSPFIGAQAGANDLAIWPGAAIYPSANATIYRVYEYAVPVTTVATVPSAYPTTAGIPGIAGYPGWYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE7</accession>
    <name>Q67LE7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196637.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2514"/>
    <dbReference type="EnsemblBacteria" id="BAD41499">
        <property type="protein sequence ID" value="BAD41499"/>
        <property type="gene ID" value="STH2514"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2514"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1676963_0_0_9"/>
    <dbReference type="OMA" id="FIWLVVE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="18053" checksum="FDC77B693A595976" modified="2004-10-11" version="1">MAEVRIRPFDYVTDTAEILSFMPELYESNFPGFVVDVDFMARRRNQLRAAARDPSQMVLVAEDPLGLCGFIWLVVEQDYRHRRRGEVSAIYVHPRMRGRGVGRRLMEAGEEYLRSSGCHSVMLMVTAANQVALGLYESLGYEITRHQMEKRLPRGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN0</accession>
    <name>Q67LN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2431</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196554.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2431"/>
    <dbReference type="EnsemblBacteria" id="BAD41416">
        <property type="protein sequence ID" value="BAD41416"/>
        <property type="gene ID" value="STH2431"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2431"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112782_0_0_9"/>
    <dbReference type="OMA" id="LGYEWMT"/>
    <dbReference type="OrthoDB" id="1515042at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="174" mass="18969" checksum="05AD2E778FD49F92" modified="2004-10-11" version="1">MTNIFQGPKSSWIWLVIRLYVGYQWLVAGWHKIVDGFDASGFLKGAIAKAVPAAEGAKPVVQGWWAAFLEGFALPNVGLFNFLIPWGEFLVGLALILGFATIFAATMGMVMNFAFLLSGTISTNPNYLILQFILVSLGGAYAGYIGIDYYFRPVWRKAIARILPGEGNAAAQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR4</accession>
    <name>Q67LR4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2397"/>
    <dbReference type="EnsemblBacteria" id="BAD41382">
        <property type="protein sequence ID" value="BAD41382"/>
        <property type="gene ID" value="STH2397"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2397"/>
    <dbReference type="HOGENOM" id="CLU_1884754_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="2">
        <location>
            <begin position="34"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14814" checksum="0A455EFA196869AA" modified="2004-10-11" version="1">MRVVTSAPRQEMLWDERNLSRDQGKGAVLGYMFDSAEFAALREEYLQGARERAGRLREAVAALREGGPVDLRQLRQEVHKLRGSGGFYGFRALSAAAAAAEDALLMVLDGEQERDDHALADLVARVVAEIEAATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ3</accession>
    <name>Q67LZ3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196441.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2318"/>
    <dbReference type="EnsemblBacteria" id="BAD41303">
        <property type="protein sequence ID" value="BAD41303"/>
        <property type="gene ID" value="STH2318"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2318"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031866_2_0_9"/>
    <dbReference type="OMA" id="FDHRAKV"/>
    <dbReference type="OrthoDB" id="692608at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="44"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="414" mass="43689" checksum="1C02F0DE607AA1EF" modified="2004-10-11" version="1">MTQALTLCTQLDRTCISPSGGEVYLLVTVKAPRMPAPEGRPPLNLAAVVDRSGSMAGAALYFTKQALRFLVDQMAEEDRLAIVTYDDQVHVPFPSQPVVQKDAVRLLVDGITAGGTTNLSGGLATGMQQIRPHAGPGRVSRVLLMTDGLANVGVTDPDVLAGWARAWREKGLAVSTMGVGPHFSEDLLVALAEAGGGNFHYIANPDQIPRIFQEELHGLLQVAVQGLHLIIETESGVAVSGVLGYRSQGTPLRAALSLPDLYAGEVKHVLVRLSVAAPPAGGKLGRVALHYLPAAAGGRPGTLEADVSLEVTDDPARLGEPPDETVMRQLRLSQAGAAWDEAVELADGGDLQGAAMRLFAAAEALEAEAAGGDSHALEQARALRAQAEAIAAAPYDTDVRKRMRQEGLRRRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M71</accession>
    <name>Q67M71_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41224">
        <property type="protein sequence ID" value="BAD41224"/>
        <property type="gene ID" value="STH2239"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2239"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="570" mass="60253" checksum="9559BF2FA8F03D02" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ0</accession>
    <name>Q67MJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196243.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2118"/>
    <dbReference type="EnsemblBacteria" id="BAD41103">
        <property type="protein sequence ID" value="BAD41103"/>
        <property type="gene ID" value="STH2118"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2118"/>
    <dbReference type="HOGENOM" id="CLU_2439680_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="90" mass="10222" checksum="A05A74292F3DB057" modified="2004-10-11" version="1">MSKSYQRWMLICTDCAYIIAAALAVWAVPLLYQLGPLAVLPYVGIAGMVWSAVTAAGLAVIWFRHERPDSRFRVRLTAWVRRPAWRESEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ2</accession>
    <name>LEUC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC</name>
        <name type="ordered locus">STH2116</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196241.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MJ2"/>
    <dbReference type="SMR" id="Q67MJ2"/>
    <dbReference type="STRING" id="292459.STH2116"/>
    <dbReference type="EnsemblBacteria" id="BAD41101">
        <property type="protein sequence ID" value="BAD41101"/>
        <property type="gene ID" value="STH2116"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2116"/>
    <dbReference type="eggNOG" id="COG0065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_9"/>
    <dbReference type="OMA" id="CNMSIEM"/>
    <dbReference type="OrthoDB" id="749418at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076827" description="3-isopropylmalate dehydratase large subunit">
        <location>
            <begin position="1"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01026"/>
        </source>
    </evidence>
    <sequence length="481" mass="51567" checksum="7E09D935EBC42B36" modified="2004-10-11" version="1">MGQGVIGMSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVRRPDLTIATMDHNVPTTDRSLPLTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIGPELGLTQPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLWQHKPKVMEIRVNGKLPPGTTAKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGMIAPDETTFAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGTNPGQVVPVTGRIPDPRDFADPAQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRIEDLRAAAAVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLDQIFREAGFEWREAGCSMCLGMNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRELLAEGGAAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP0</accession>
    <name>MTNA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="1">M1Pi</shortName>
            <shortName evidence="1">MTR-1-P isomerase</shortName>
            <ecNumber evidence="1">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtnA</name>
        <name type="ordered locus">STH2068</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196193.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MP0"/>
    <dbReference type="SMR" id="Q67MP0"/>
    <dbReference type="STRING" id="292459.STH2068"/>
    <dbReference type="PRIDE" id="Q67MP0"/>
    <dbReference type="EnsemblBacteria" id="BAD41053">
        <property type="protein sequence ID" value="BAD41053"/>
        <property type="gene ID" value="STH2068"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2068"/>
    <dbReference type="eggNOG" id="COG0182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_9"/>
    <dbReference type="OMA" id="RLWVDET"/>
    <dbReference type="OrthoDB" id="1290468at2"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00874"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000357245" description="Methylthioribose-1-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="359" mass="39604" checksum="F6566DE1F6BA11A2" modified="2004-10-11" version="1">MPKQIEPVALDDAGSALVIVDQTLIPNETRYLRLTTPEETWEAIRSLRVRGAPAIGIAAAMGLYLGVKGSEAADFEDFYHEFRQVKAYLASARPTAVNLFWALDRMEACLLRHRDRPIPEIKEALRAEAEAIREEDARANRTIGEYALSLLKPGMGILTHCNAGALATAAYGTALAPIYLGQERGYNFRVFADETRPLLQGARLTAYELMQAGVDVTLICDNMASAVMKNGWVDAVFVGCDRVAANGDTANKIGTSGVAILARHYGIPFYVCAPTSTIDLRCPTGADIVIEERRPEEVTEQWYAKRMAPEGVKVYNPAFDVTDADLITAIITEYGIARPPYTESLKELFRRKEEAERRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP9</accession>
    <name>Q67MP9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin-like_fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196184.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41044">
        <property type="protein sequence ID" value="BAD41044"/>
        <property type="gene ID" value="STH2059"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2059"/>
    <dbReference type="HOGENOM" id="CLU_207734_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like_fold" evidence="1">
        <location>
            <begin position="2"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="63" mass="6747" checksum="65C85026953FC807" modified="2004-10-11" version="1">MAAMENPRIRAYAVDAAAFGELARRNGVRSVPHTIIEGARRVTLAGGGPEERFLAALLQAARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV1</accession>
    <name>Q67MV1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2007</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196134.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2007"/>
    <dbReference type="EnsemblBacteria" id="BAD40992">
        <property type="protein sequence ID" value="BAD40992"/>
        <property type="gene ID" value="STH2007"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2007"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="VDCAYDG"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25883" checksum="768E33CA3F110F2A" modified="2004-10-11" version="1">MPHILVVDDEPGLVKGLRLSLEQEGFQVTTASDGREALSKFRAGDFDLIVLDLMLPGVDGLTICREVRAAGMTPIIMLTAKADDIDKILGLELGADDYITKPFNTRELIARIRAVLRRAEAASRQDRAGRLVVGDLVLHPRQRRAEVAGRPLELTAREYDLLELLARHPGVVYTRQELLDLVWGYDFAGDERTVDVHVRRIREKLEEDPSRPRYLRTKWGVGYYLEAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW2</accession>
    <name>Q67MW2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1996</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1996"/>
    <dbReference type="EnsemblBacteria" id="BAD40981">
        <property type="protein sequence ID" value="BAD40981"/>
        <property type="gene ID" value="STH1996"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1996"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_12_0_9"/>
    <dbReference type="OMA" id="QWCVEDD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="72"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="142" mass="15033" checksum="89A6A2C871334C4A" modified="2004-10-11" version="1">MRLRDLMTTDVRTCAPDTPVSEVARIMEEADCGFVPVVDGGRVAGVITDRDIVLRAVARGRDIRTTTARECMTSPAVTVGPDTDAHAAADLMADKQIRRLCVVDGGRLVGVVALGDLATERIHVDEAGEALSSISEPAQPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX5</accession>
    <name>Q67MX5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1983</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196110.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1983"/>
    <dbReference type="EnsemblBacteria" id="BAD40968">
        <property type="protein sequence ID" value="BAD40968"/>
        <property type="gene ID" value="STH1983"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1983"/>
    <dbReference type="eggNOG" id="COG1482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020529_0_0_9"/>
    <dbReference type="OMA" id="FRPKIWG"/>
    <dbReference type="OrthoDB" id="645703at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014628">
        <property type="entry name" value="Man6P_isomerase_Firm_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036894">
        <property type="entry name" value="PMI_Firm_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="324" mass="35095" checksum="C7848840F13E0991" modified="2004-10-11" version="1">MLYPLRFTPLFKERVWGGRRLARFYPDLPPGPIGEAWVLSDHPQGRTPIANGPWAGETLSGLREHLPPERDAALMGARVLARGEEGFPLLFKLLDAQDDLSIQVHPADDFPGLPPGERGKTEMWVVLEAEPGARIVHGLAEGVTRDGLAAAVREGRTVEAMRFLTVRPGDVYYLPAGLVHALGAGLVVAEIQQSSDTTYRLYDYGRLGLDGKPRELHIDQALACCSDAPPPAPAQLGLPEPNRWETLVSSPYFVTAHGRCRGDWPQETDPGTFEALMILSGAGAIAWPGGGEEPLAPGQAWLVPAALGPYRLSGEFDALRVRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ5</accession>
    <name>Q67MZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1963"/>
    <dbReference type="EnsemblBacteria" id="BAD40948">
        <property type="protein sequence ID" value="BAD40948"/>
        <property type="gene ID" value="STH1963"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1963"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148636_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="15963" checksum="5F61E3220C4C483A" modified="2004-10-11" version="1">MEDLRIRFAGLEDEETCRRMDPYLPGPAFAHKIALHELLLVERAGRTIGYVRLEYIWGKIPYIGLIWLEPTHRGQGIGRAVLAFVEAHLRERGYTFLLSSCQVNEPEPQAWHRAVGFRECGLIAGINDGGIGEVFFRKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ6</accession>
    <name>Q67MZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1962"/>
    <dbReference type="EnsemblBacteria" id="BAD40947">
        <property type="protein sequence ID" value="BAD40947"/>
        <property type="gene ID" value="STH1962"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1962"/>
    <dbReference type="HOGENOM" id="CLU_1874385_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="14262" checksum="D81EEEF4315A77C5" modified="2004-10-11" version="1">MFSGGTAVRPRACCGGQIGASCSVYDEVDGVRLTDLPPPEVEDPGNTQWNRVPCRIRLEPVRSTPHWEDARPELAGPGLGENAWEAYNAARQVFAPAPQGGEPAGRLARLEPGPGVALVSGMRPAHAADLAAGLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N30</accession>
    <name>Q67N30_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenosylcobinamide kinase</fullName>
            <ecNumber evidence="8">2.7.1.156</ecNumber>
            <ecNumber evidence="9">2.7.7.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Adenosylcobinamide-phosphate guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">STH1928</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide + ATP = adenosylcob(III)inamide phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15769"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosylcob(III)inamide + GTP = adenosylcob(III)inamide phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15765"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosylcob(III)inamide phosphate + GTP + H(+) = adenosylcob(III)inamide-GDP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.7.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CobU/CobP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.156" evidence="8"/>
    <dbReference type="EC" id="2.7.7.62" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196055.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1928"/>
    <dbReference type="EnsemblBacteria" id="BAD40913">
        <property type="protein sequence ID" value="BAD40913"/>
        <property type="gene ID" value="STH1928"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1928"/>
    <dbReference type="eggNOG" id="COG2087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094161_0_1_9"/>
    <dbReference type="OMA" id="DCLTVWL"/>
    <dbReference type="OrthoDB" id="1903913at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00236"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043752">
        <property type="term" value="F:adenosylcobinamide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00544">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003203">
        <property type="entry name" value="Cobinamide_kinase/P_G-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34848">
        <property type="entry name" value="PTHR34848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02283">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006135">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="16">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="BAD40913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="183" mass="19948" checksum="6C3D28AD101E1912" modified="2004-10-11" version="1">MAGVILVLGGARSGKSRWAEHLASPFRRVVYLATGRAGDAEMADRIARHRASRPAHWRTVEEELRPAAALAQALHEAPADAVLLDCVTLLISNHLLQGEEGFEARARQELAQLLSLTRERGALLIAVSNEVGAGLVPEHRLGRLFRDAQGRLNQWLAREADQVYACVAGIAVDLRRIGTVIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N59</accession>
    <name>Q67N59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196026.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1899"/>
    <dbReference type="EnsemblBacteria" id="BAD40884">
        <property type="protein sequence ID" value="BAD40884"/>
        <property type="gene ID" value="STH1899"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1899"/>
    <dbReference type="eggNOG" id="ENOG50330BE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128689_1_0_9"/>
    <dbReference type="OMA" id="ANRLKLW"/>
    <dbReference type="OrthoDB" id="1879632at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025689">
        <property type="entry name" value="Spore_YtrH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14034">
        <property type="entry name" value="Spore_YtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="109" mass="11440" checksum="C6E3F2389A48D903" modified="2004-10-11" version="1">MDDGFAAKLLSNFLIALGVTLGGSLSGAAASLLSGGHPLDRLRYLSEDLRLWGILVAVSGSFEPLRTLEQGLLFGQGRALVRQVVALVVAMAGANVAYFLIQTVAGRRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-19" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N73</accession>
    <name>DNAE2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Error-prone DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaE2</name>
        <name type="ordered locus">STH1885</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-C family. DnaE2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196012.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N73"/>
    <dbReference type="SMR" id="Q67N73"/>
    <dbReference type="STRING" id="292459.STH1885"/>
    <dbReference type="EnsemblBacteria" id="BAD40870">
        <property type="protein sequence ID" value="BAD40870"/>
        <property type="gene ID" value="STH1885"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1885"/>
    <dbReference type="eggNOG" id="COG0587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001600_0_0_9"/>
    <dbReference type="OMA" id="NSWPMGF"/>
    <dbReference type="OrthoDB" id="561611at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01902">
        <property type="entry name" value="DNApol_error_prone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041931">
        <property type="entry name" value="DNA_pol3_alpha_thumb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023073">
        <property type="entry name" value="DNA_pol_error_prone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00594">
        <property type="entry name" value="polc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000103400" description="Error-prone DNA polymerase">
        <location>
            <begin position="1"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01902"/>
        </source>
    </evidence>
    <sequence length="1085" mass="120631" checksum="1BC11470BF0DB77E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N95</accession>
    <name>Q67N95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1863"/>
    <dbReference type="EnsemblBacteria" id="BAD40848">
        <property type="protein sequence ID" value="BAD40848"/>
        <property type="gene ID" value="STH1863"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1863"/>
    <dbReference type="HOGENOM" id="CLU_2371708_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="95" mass="10403" checksum="1EF0D4D2E82ADD50" modified="2004-10-11" version="1">MLGDIAEEIEEITEFQGDLAEYVDEMDDDLSSVEDEVYNENEIVFIPDDADDVTVLTCSRCGEPIGAQAGEVDDEDLEVTCPVCGCTMEADEVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N98</accession>
    <name>Q67N98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage III sporulation protein AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoIIIAC</name>
        <name evidence="2" type="ordered locus">STH1860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1860"/>
    <dbReference type="EnsemblBacteria" id="BAD40845">
        <property type="protein sequence ID" value="BAD40845"/>
        <property type="gene ID" value="STH1860"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1860"/>
    <dbReference type="HOGENOM" id="CLU_194471_2_0_9"/>
    <dbReference type="OMA" id="HWVTLIG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009570">
        <property type="entry name" value="Spore_III_AC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025664">
        <property type="entry name" value="Spore_III_AC/AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06686">
        <property type="entry name" value="SpoIIIAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02848">
        <property type="entry name" value="spore_III_AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7388" checksum="0D629B9E4562999D" modified="2004-10-11" version="1">MSRVDVQLLFRIAGVGIIVAVMATVLKQAGKDEQGQMVTLVGVLVVLMMVVTLLGRFFNLVKATFGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND2</accession>
    <name>Q67ND2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable butyrate kinase</fullName>
            <shortName evidence="8">BK</shortName>
            <ecNumber evidence="8">2.7.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Branched-chain carboxylic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">buk</name>
        <name evidence="10" type="ordered locus">STH1826</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + butanoate = ADP + butanoyl phosphate</text>
            <dbReference type="Rhea" id="RHEA:13585"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58079"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.7" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195954.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1826"/>
    <dbReference type="EnsemblBacteria" id="BAD40811">
        <property type="protein sequence ID" value="BAD40811"/>
        <property type="gene ID" value="STH1826"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1826"/>
    <dbReference type="eggNOG" id="COG3426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048716_0_0_9"/>
    <dbReference type="OMA" id="IWHALNQ"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047761">
        <property type="term" value="F:butyrate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00542">
        <property type="entry name" value="Butyrate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011245">
        <property type="entry name" value="Butyrate_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060:SF3">
        <property type="entry name" value="PTHR21060:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036458">
        <property type="entry name" value="Butyrate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02707">
        <property type="entry name" value="butyr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="361" mass="38111" checksum="228F857F8E8AC813" modified="2004-10-11" version="1">MQQRLLIINPGSTSTKIAVYDDDSPLFAETLRHSAADLACFRDVAAQFPFRRDLILEALDAHGVSLSSLTAVVGRGGLLRPVRGGTYRVDAAMLAELSRAAHGEHASNLGALLAHEIAQAAGGVPAFIVDPVVVDELEPVARLTGLPEMPRRSVFHALNQKAVARRAAADLGRAYTEVNLVVVHLGGGISVGAHRRGRVVDVNNALDGEGPMAPERAGTVPSLGLVHLAFSWQHTLREVGRMLVGRGGLVAHVGTNDARRVEERIAAGDEAARVAYRAMAYQVAKEVGRAAVALGGQVDRIVLTGGLAHSEMLTGWIAEQVEWIAPVAVYPGEDEMAALAAGALRVLRGEEPAQVYGEAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH6</accession>
    <name>Q67NH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar ABC transportor ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1782</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195910.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1782"/>
    <dbReference type="EnsemblBacteria" id="BAD40767">
        <property type="protein sequence ID" value="BAD40767"/>
        <property type="gene ID" value="STH1782"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1782"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="SPKAFLM"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03301">
        <property type="entry name" value="ABC_MalK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015855">
        <property type="entry name" value="ABC_transpr_MalK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="364" mass="40884" checksum="8499BF5145C9B383" modified="2004-10-11" version="1">MAEVSLRHVSKKFGDVTVVRDFNLDIADREFIVFVGPSGCGKSTTLRMIAGLEEITEGEIYIGDRLVNNVAPKDRDIAMVFQNYALYPHMNVYENMAFGLKLRRVPKAEIDRRVKEAAAILGLSDLLGRKPKSLSGGQRQRVALGRAIVRNPKVFLMDEPLSNLDAKLRASMRTELAKLHERLQTTTIYVTHDQVEAMTMGHRIVVMRDGVIQQVAPPQELYDRPANLFVAGFIGSPAMNFLRGRLSEDGQVFRAATFQFRLPERLAAAARPYAGKEMILGIRPENIHADPTYLATHDGPRIPARIEVVEPLGAEIYLYLRTGEDEVTARVEPHLRFRPHDQLDLAFDADKVHLFDAATEMAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK7</accession>
    <name>Q67NK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1751</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195879.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1751"/>
    <dbReference type="EnsemblBacteria" id="BAD40736">
        <property type="protein sequence ID" value="BAD40736"/>
        <property type="gene ID" value="STH1751"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1751"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_9_1_9"/>
    <dbReference type="OMA" id="SYACYTM"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="4">
        <location>
            <begin position="10"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="4">
        <location>
            <begin position="158"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="326" mass="34196" checksum="6C7382FC4293DACE" modified="2004-10-11" version="1">MERTRGRQTLGYMMVFGAASLWGTLGVVSRNLYVGGLSAQAVVTLRASMAAALLLAALTVLRPATLRVRLRDIPLFAAYGLVSVAAFYLLYFLTIQHLSVAAAAVLMYTAPAFVAVAAALTLGEPLTQGKLLALTLTMAGCALVARAYEPSVFRGQLIGVLTGLGSGFTYGMYSIFGKHALKRYNPWTVQAYSLLFGAAPLVLFFGREAVRSLARSPEALPWLAYLALVTTLGAYGLYLSGLQVVEASHAALLSTVEPVVAALLGFWILEESLRLPQVIGILLVLGSAVILNAQRGEPAGAASRQAGVPPAGVGTECARRTPGGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL6</accession>
    <name>Q67NL6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1742</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195870.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1742"/>
    <dbReference type="EnsemblBacteria" id="BAD40727">
        <property type="protein sequence ID" value="BAD40727"/>
        <property type="gene ID" value="STH1742"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1742"/>
    <dbReference type="eggNOG" id="COG1668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091276_1_0_9"/>
    <dbReference type="OMA" id="VPIGVVW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="278" mass="29524" checksum="95385927C9FE6E9F" modified="2004-10-11" version="1">MNRRAVLAVARKDIRAVTANLQVWLPMVLVPLILGVAVPLGMVLAFRYGAEGLSAADVRTLIGWAERLPAGDLGDALAGLPDLSRRLIYVGANYLLAPFFLMIPLMAASVISADSFAGEKERGTLETLLFAPVDMVSLFTGKVLAAFAPAMGLSLATLLLCAAAVNAAAWPLFHRIIFPQPNWIPLVLLVIPAVALLAILLNVFISARVATFQAAYQMGGLVVLPMLLLVAGQATGVLVFSVPVVLAVGLGLWLVDGLLLWLALGRLDRQQLFAGQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM7</accession>
    <name>Q67NM7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1731"/>
    <dbReference type="EnsemblBacteria" id="BAD40716">
        <property type="protein sequence ID" value="BAD40716"/>
        <property type="gene ID" value="STH1731"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1731"/>
    <dbReference type="eggNOG" id="COG1333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034630_1_0_9"/>
    <dbReference type="OMA" id="TEVHINR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023494">
        <property type="entry name" value="Cyt_c_bgen_Ccs1/CcsB/ResB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007816">
        <property type="entry name" value="ResB-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566">
        <property type="entry name" value="PTHR31566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05140">
        <property type="entry name" value="ResB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="zinc_ribbon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="zinc_ribbon_2" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="ResB" evidence="7">
        <location>
            <begin position="70"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="506" mass="57439" checksum="CBF7D6F84AB1555E" modified="2004-10-11" version="1">MWLDWQGGSFALAAVHAANCPDCQRPLPAGAHFCPHCGRALGDRSRARVEGPNPWVVLLDRTWDFFASTKVASWIIIALAIASIAGTVIEQEGMYQDWRPPELYYPDRYGEFWGSLFLRLGLTHAYSSWWYAALVMMLVISLVICSLHRLVPLHRQLTRPQVWKLPQYLRKQQVVLEVPGDLQQAEERLKRAGYRIWRDRECLYAEKGRLSRYGPYILHIGLLVVCFAAFAKALPGWDEVQDVWIPDGQTVKVPGENFAITNHKFTMELYENGMPSRYATDASIIVDGEEVLRRTIEVNKPLKYGGWEIYQASWREEPGVAHLRLVEADTGSAVATVAFDLRDPSPEYSLEGTGLKLVVLTYLHDFVLDPETNQPTNASYEVRNPVLFAEVATADGSEMVGRIALAMAGGSEVVYTGPYYLELDRVESRWYTALKLHHDRTVPYMYFGLAVVFVGMVITFFIYHWQVWVREENGGILIGARAYKNRFALAREIKRIFGAPQGEGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP1</accession>
    <name>Q67NP1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195845.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1717"/>
    <dbReference type="EnsemblBacteria" id="BAD40702">
        <property type="protein sequence ID" value="BAD40702"/>
        <property type="gene ID" value="STH1717"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1717"/>
    <dbReference type="HOGENOM" id="CLU_1937072_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023804">
        <property type="entry name" value="DUF3792_TM"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04086">
        <property type="entry name" value="TIGR04086_membr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="130" mass="12793" checksum="4ABDCA17DF573255" modified="2004-10-11" version="1">MRRADGGEALVDLGALASGVLWGLLLMVAAALTLGVLDFWFPPEPQAEAARTLIVQGVAAALAGARSTWLAGRGGFMHGLAAGVGLVAAVTVIIGIFRDLPGILGLARGLAVGAGAGALAGVAAVSLGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ8</accession>
    <name>Q67NQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195828.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1700"/>
    <dbReference type="EnsemblBacteria" id="BAD40685">
        <property type="protein sequence ID" value="BAD40685"/>
        <property type="gene ID" value="STH1700"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1700"/>
    <dbReference type="HOGENOM" id="CLU_1045558_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="28798" checksum="886785EBFF8A74B2" modified="2004-10-11" version="1">MEFLLLYDLHLFAQVEVAGQKRLANIDTGANIDLAFGVFEGVEMLDGEEGPVHGAVGEVRLRSGRVPDVRLLDEQVGPLQVAISDENGYEKHPFPVSMRLGAKTLLSRPLLLDFKGLRMGRERLPDSYPRVAAPLEFVCGVPFVEIELGGRRLRALFDTAAGMSVLNTAHLDDLGLRPEVLYETQATDSSGTTVTLPVGRTRGLQIGGLALGDCEYLMLDLSAIEEIMGTRIDLGLGLNTLLASSTVWYLDPQAGRVQVTDVGLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ1</accession>
    <name>Q67NZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1617"/>
    <dbReference type="EnsemblBacteria" id="BAD40602">
        <property type="protein sequence ID" value="BAD40602"/>
        <property type="gene ID" value="STH1617"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1617"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="YLGAHHD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="25382" checksum="46447A2E836A1F68" modified="2004-10-11" version="1">MLEPGEVVGLIGPNGAGKTTVFNVITGVFPPTEGEVLLEGRVLNGLRPWRITQLGIARTFQNIRLFREMSVLDNVLAAMHPRCGYGLLDALLHTPRFAGREQAMRDEALALLERMNLADRRHDRAGSLPYGAQRRLEIARALATHPKVLLLDEPAAGMNPTEVDALVELIRGVAAEFGLTVLLIEHQMGLVMNLCQRLVVLDFGEVIARGKPTEIQQNPRVLEAYLGRGVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P59</accession>
    <name>DAPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="1">HTPA synthase</shortName>
            <ecNumber evidence="1">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapA</name>
        <name type="ordered locus">STH1549</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P59"/>
    <dbReference type="SMR" id="Q67P59"/>
    <dbReference type="STRING" id="292459.STH1549"/>
    <dbReference type="EnsemblBacteria" id="BAD40534">
        <property type="protein sequence ID" value="BAD40534"/>
        <property type="gene ID" value="STH1549"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1549"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_9"/>
    <dbReference type="OMA" id="MHELMFN"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000103170" description="4-hydroxy-tetrahydrodipicolinate synthase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="293" mass="31202" checksum="220526EFBDEEFDCA" modified="2004-10-11" version="1">MPTFGRLLTAMVTPMTPDGAVDYQRAGELAKHLVAAGSEGLVVSGTTGESPTLSHEEKLRLFETVVDAVGGQVSVIAGTGSNNTAESIRFSREAARTGVHGLLLVTPYYNKPPQEGLYRHFRAVAEAVDLPCILYNVPSRTSVNMLPATTLRLARDVPNIVGIKECADVGQLADILSGAPEGFRVWSGDDANLLPYLTVGAYGIISVASHVVGPQMRELIDAFLAGDTARAAAWHRRLLPVFRGLFAVTNPILVKAALRLTGFPVGPVRLPLVDATEEQEEALREVLAEAGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA3</accession>
    <name>Q67PA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1505"/>
    <dbReference type="EnsemblBacteria" id="BAD40490">
        <property type="protein sequence ID" value="BAD40490"/>
        <property type="gene ID" value="STH1505"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1505"/>
    <dbReference type="eggNOG" id="COG4454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1093850_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028096">
        <property type="entry name" value="EfeO_Cupredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13473">
        <property type="entry name" value="Cupredoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupredoxin_1" evidence="1">
        <location>
            <begin position="160"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="27900" checksum="9C54FCF661C025D0" modified="2004-10-11" version="1">MHRFLSRAGGDTVVQQRTLPHQGGMQMKQWLRRAVAAMTAAVLLAVMAGRALGAEEEADADALAHRLQDAREATPDLVAAAAARDLERFRSAYNRFAAALDPVLPAVRAEDPRLAARLVVASDALRGLALAGILSEEDVAREVAVLRGALEEALRAAARSTERVQRFTVAAREYRFEPDELRARPGTRIVVRLENRGRVPHEFRIPELNVVIGPVEPGRSAEGEFIVERPGTYGYECHVDGHYLKGMRGRLVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB1</accession>
    <name>Q67PB1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="12">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH1497</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1497"/>
    <dbReference type="EnsemblBacteria" id="BAD40482">
        <property type="protein sequence ID" value="BAD40482"/>
        <property type="gene ID" value="STH1497"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1497"/>
    <dbReference type="eggNOG" id="COG4589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037294_3_1_9"/>
    <dbReference type="OMA" id="FTFDEVG"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="30307" checksum="14E295B3BAA59A0B" modified="2004-10-11" version="1">MSGASAPCEPEGAGSRLSHSKAGDVVLVRILTGLVGIPLFLGLLFIGQWPFLVVVAGMGLIGFYEYARILRAMGFRPSLPLGCAATVAIVLAAGLLPPHGVHAQAVAALATLVILAWAIFRPEGFSALDGLLTLAGVAYVGLLSYLVLLRRLGGGSGWDLGLVWAGMAFLCTWAADTLAYFVGVTWGRHKLAPKISPQKSVEGLLGGVAGALLAGLLWGPAIPLARWQGALLGAAIALVAAMGDLVESALKRTAGVKDAGRLLPGHGGVLDRFDSSLFVLPFVYYVATLLFGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH0</accession>
    <name>Q67PH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1438</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195568.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1438"/>
    <dbReference type="EnsemblBacteria" id="BAD40423">
        <property type="protein sequence ID" value="BAD40423"/>
        <property type="gene ID" value="STH1438"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1438"/>
    <dbReference type="eggNOG" id="COG0742">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075826_0_2_9"/>
    <dbReference type="OMA" id="FNWLMPY"/>
    <dbReference type="OrthoDB" id="1827334at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="20707" checksum="273E3ACA1377DF56" modified="2004-10-11" version="1">MRVITGSAKGRPLKTVKSRAVRPTSDRVKESLFNIIGSRVVDADFLDLFAGSGAVGIEALSRGARACVFVELQTAHLKVVADNLRTTGLAGRAELIRRDARAALVDLAHRGRRFDFIFVDPPYGQDLVPAVLALIDGHGVLAEDGWVICEHHAKDPVPAAAGGLYRFREVLFGETMLSIYRADARDAGGQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI5</accession>
    <name>Q67PI5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1423</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195553.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1423"/>
    <dbReference type="EnsemblBacteria" id="BAD40408">
        <property type="protein sequence ID" value="BAD40408"/>
        <property type="gene ID" value="STH1423"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1423"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_2_9"/>
    <dbReference type="OMA" id="PVQYWHW"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="94"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="33543" checksum="3EE3705BA73D251D" modified="2004-10-11" version="1">MGRYLVRRLAHAGLVVIGVTLVAFFLTRMTGDPVRLLAPLDATEEEVELLRTQLGFNRPLYVQFLEYMRGVLVLDFGESLRHGEPAMRLVLERMPATLQLSLAALLLALAVAIPIGVVSATRRGGLLDGLGTLLALLGQSMPVYWLGILLIIVFGVQLRWLPAGGRAGWTSYILPAVTLAAYTCASIMRLLRSSLLNVLSQDFIRTARAKGLEERRVIYRHALRNALLPVVTVVGLQMGSLLSGSVITETIFAWPGVGRFVVQAIFNRDFYVVQAAVFLFALIIVAINLVTDLVYALLDPQIRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ6</accession>
    <name>Q67PQ6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1352"/>
    <dbReference type="EnsemblBacteria" id="BAD40337">
        <property type="protein sequence ID" value="BAD40337"/>
        <property type="gene ID" value="STH1352"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1352"/>
    <dbReference type="eggNOG" id="COG0631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034545_4_1_9"/>
    <dbReference type="OMA" id="EDHTWVA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="245" mass="26486" checksum="2B4B14840B99452D" modified="2004-10-11" version="1">MYGERTDPGRVRPSNQDAYLFEPLSVPGGGALLAVADGMGGYDGGEVASRLAIQTLRAYVPPRVEAWVREGSVGEGLVEAIHEANHAILQEQMARAELAHMGTTVTAVVVMGPRLYVGHVGDSKAFLVGPREARQITSDHNVPGELLQSGHLTREEAANHPQRHVLTRALGVRPDLPVEQRELEWAVGDLLLLCTDGLSNLVALEEIREAALRLEPDALAEWLVNLANSRGGYDNITVLAARWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV4</accession>
    <name>Q67PV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195435.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1304"/>
    <dbReference type="EnsemblBacteria" id="BAD40289">
        <property type="protein sequence ID" value="BAD40289"/>
        <property type="gene ID" value="STH1304"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1304"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1049408_0_0_9"/>
    <dbReference type="OMA" id="HAKCAVA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="1">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="28553" checksum="A08E38C965D968D9" modified="2004-10-11" version="1">MNPKLELSLVALAESAGDDALAALRALLAAERIGPGTTLHEVRTLLAPHRIDDHLLAPFLKLWAATGATAVPGPALALALGVAHATRARAERRAPRISPVWTGPYAPPGVQARSTLAVMQEMVEGARARILLVGYALTGESPEAVRLVLALARRAAQGVQVAIALHDDGKNHDTLLNLWPERIPLSLLRWTGRPDVPKASLHAKLLVVDSRDLLVTSANLTHLGLDKNIEFGVRIQGQVAHEIDRHFAALVRDAVLVEYDRSQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV9</accession>
    <name>Q67PV9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1299"/>
    <dbReference type="EnsemblBacteria" id="BAD40284">
        <property type="protein sequence ID" value="BAD40284"/>
        <property type="gene ID" value="STH1299"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1299"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_7_0_9"/>
    <dbReference type="OMA" id="WHERLGG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="76"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="29335" checksum="2EFCD83E59CF8BD2" modified="2004-10-11" version="1">MRQQQQPELQSLSFEERLELLVDQEWLDRQNRRLARLLKEAKFRVAACMEDVDYQHPRGLDRTVMRSLAACQWVEYGQHILIAGPTGVGKTFIACALGNAACRHGYTVRYYRVPRLLAELATARVDGSLRRILNTLARTDVLILDDWGLAPISVAESRDLLEVIDDRTQARSTVIASQLPIETWHSVIGDATVADAILDRLVHGAHKLNLKGESMRKIANLHHGLALPSHRHFAPSPQCHRAGRMKSETAVDLDQNRWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX3</accession>
    <name>Q67PX3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1285</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195416.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1285"/>
    <dbReference type="EnsemblBacteria" id="BAD40270">
        <property type="protein sequence ID" value="BAD40270"/>
        <property type="gene ID" value="STH1285"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1285"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_1_0_9"/>
    <dbReference type="OMA" id="AWKRGVH"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269608" evidence="8">
        <location>
            <begin position="43"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="337" mass="34789" checksum="199AC4E8350B0594" modified="2004-10-11" version="1">MSSRNPAKVHRPAALLLLAGVLVLVALLSLRVGSIPVSTADAVAALTRYDPDNYSQLVVRTMRLPRTVIGLGVGAALAAAGAAMQAATRNPLAEPSLLGVNAGASFAIVAAVYFGHMTHPLQYLWFAFAGALGAAVLVYAIASAGGGASPVKLALAGVIVSALLGSWTTALLILNKETLEMVRFWLAGSLAGRSLNLLPPVAPFLAAGLLGVLLVGRQLNTLSLGEETARSLGMHTGRMRALVAVLVMLLAGSAVALAGPVGFVGLAVPHIVRAWIGADYRWILPFCLVLGPALLLGADILGRVMMRPAEVQVGVVTALVGAPFLIYLARRRQVAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PZ8</accession>
    <name>Q67PZ8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="9">DHOD</shortName>
            <shortName evidence="9">DHODase</shortName>
            <shortName evidence="9">DHOdehase</shortName>
            <ecNumber evidence="9">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrD</name>
        <name evidence="11" type="ordered locus">STH1260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-dihydroorotate + A = AH2 + orotate</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="9">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195391.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1260"/>
    <dbReference type="EnsemblBacteria" id="BAD40245">
        <property type="protein sequence ID" value="BAD40245"/>
        <property type="gene ID" value="STH1260"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1260"/>
    <dbReference type="eggNOG" id="COG0167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_0_0_9"/>
    <dbReference type="OMA" id="SCPHAKG"/>
    <dbReference type="OrthoDB" id="1109542at2"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 9" id="KW-0288">FMN</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="10">
        <location>
            <begin position="8"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="73"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="49"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="31665" checksum="26900AB7746A75CA" modified="2004-10-11" version="1">MASDQPDLAVQLFGRTFRTPVWAASGCFGYGREFAGVLDLSRVGAICVKGTSIEPWTGNPGIRVVETPGGMLNAIGLQNPGVDHFLTVDWPWLQEQGATVIVNIVGRTVEEYVAVARRLEGTGVAAVELNISCPNVKEGGMAFGTTPGSAAAVTAAVRKATTLPLIVKLSPNVADVVAIARAVEDAGADGLSVINTLQGMSIDIRRGRPHLGNVFGGLSGPAIKPVALRMVWQVAGAVKVPVIGVGGIMSGEDAAEFLMAGASAVQVGTACFIDPRAPLTIAEELARFMGEHGFRNVRALVGAARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q79</accession>
    <name>Q67Q79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40164">
        <property type="protein sequence ID" value="BAD40164"/>
        <property type="gene ID" value="STH1179"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1179"/>
    <dbReference type="HOGENOM" id="CLU_1293791_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="21057" checksum="314865D77C3AA48B" modified="2004-10-11" version="1">MGLFAARRAAVGVAGVSISVLGAWAVRIIANARVTVIGCAVFGSVAVGLVAVGLTGTGISVPACDPVVGGLVGPADRVAGLLSARPTVVGVVLRLTGGVTLGEFVVVRIAGPLSVVLHRLPGRIFVLFLAAVLVAIAGFGRLIAGRSCSGGAVIGLRSLLAFLLLHDGLGGNPAVRSRIGQLAAGSVSTRICRVRGRGGATIHAVPGRNGLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB4</accession>
    <name>Q67QB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1144"/>
    <dbReference type="EnsemblBacteria" id="BAD40129">
        <property type="protein sequence ID" value="BAD40129"/>
        <property type="gene ID" value="STH1144"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1144"/>
    <dbReference type="HOGENOM" id="CLU_128747_5_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="40" mass="4379" checksum="FBEDB8B12E717AC8" modified="2004-10-11" version="1">MAVFVCTQCGAEREGRCKPQKCACGAKGTFVKKEEAKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD7</accession>
    <name>Q67QD7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Penicillin-binding protein 1A</fullName>
            <ecNumber evidence="8">2.4.1.129</ecNumber>
            <ecNumber evidence="7">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">STH1121</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="8"/>
    <dbReference type="EC" id="3.4.16.4" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195253.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1121"/>
    <dbReference type="EnsemblBacteria" id="BAD40106">
        <property type="protein sequence ID" value="BAD40106"/>
        <property type="gene ID" value="STH1121"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1121"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_9"/>
    <dbReference type="OMA" id="PWRAPYF"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Transgly" evidence="20">
        <location>
            <begin position="77"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="19">
        <location>
            <begin position="366"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="790"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="800"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="848"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="BAD40106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="953" mass="103652" checksum="100E961A0C540330" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN0</accession>
    <name>Q67QN0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydroxylamine reductase</fullName>
            <ecNumber evidence="7">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Hybrid-cluster protein</fullName>
            <shortName evidence="7">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hcp</name>
        <name evidence="8" type="ordered locus">STH1028</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="7">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HCP family.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195160.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1028"/>
    <dbReference type="EnsemblBacteria" id="BAD40013">
        <property type="protein sequence ID" value="BAD40013"/>
        <property type="gene ID" value="STH1028"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1028"/>
    <dbReference type="eggNOG" id="COG1151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038344_0_0_9"/>
    <dbReference type="OMA" id="MRPRETY"/>
    <dbReference type="OrthoDB" id="337713at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="7">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="7">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="46772" checksum="4E2B99660B9BEE8A" modified="2004-10-11" version="1">MFCNQCEMTAHGTGCTKVGICGKDENIQALQENLIYALKGIAAYTYHARELGYTDPEIDAFFAEALYSTLTNVDFDLNHFLELNMRAGQMTLRAMTMLKKAHTDHFGEMVPTQVPTGTVAGPAVVVTGHNLKALYELLRQTEGTGINVYTHSEMLPAHGYPGLKRFPHLVGNLGAAWCDQKKLFAQFPGAIVGTSNCVQIPLDAYKDRIFTVGASRLPGVPHVENWDFSPVIARALSLPPCEEKPGEVTLTTGFADTNILPMADKILAGVKAGKIRHFFVVGGCDAPGSLGNYYRDFVRMTPPDTLVITLACGKFRFNDLDLGTIDGIPRLLDLGQCNDTISAIRIATALAEAFGCEVNDLPLSIVLSWMEQKAVAVLMCLLSLGIRGIYLGPKPPAWITPDVLRVLQEQFDLRLTTTPEADLKALLGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-11-08" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ0</accession>
    <name>G6PI_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose-6-phosphate isomerase</fullName>
            <shortName evidence="1">GPI</shortName>
            <ecNumber evidence="1">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoglucose isomerase</fullName>
            <shortName evidence="1">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphohexose isomerase</fullName>
            <shortName evidence="1">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgi</name>
        <name type="ordered locus">STH1008</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195140.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QQ0"/>
    <dbReference type="SMR" id="Q67QQ0"/>
    <dbReference type="STRING" id="292459.STH1008"/>
    <dbReference type="EnsemblBacteria" id="BAD39993">
        <property type="protein sequence ID" value="BAD39993"/>
        <property type="gene ID" value="STH1008"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1008"/>
    <dbReference type="eggNOG" id="COG0166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037303_0_1_9"/>
    <dbReference type="OMA" id="CPAYAYG"/>
    <dbReference type="OrthoDB" id="417261at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000180751" description="Glucose-6-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <sequence length="448" mass="49188" checksum="209CF6D33C30A27E" modified="2004-10-11" version="1">MSSSLRFTVSPSLLGPHPHELTYQAEAIRIAHRMLHERTGPGAGFTGWLDLPERYDREEFRRIQEAAARIRGDSDVLVVIGIGGSYLGARATVEALGHAFHNLLPPERRGAPQIFFAGTDLSPRYLTELLDVLEDREVSLNVVSKSGTTTEPAIAFRLLRNWMERRYGKEGARRRIYATTDAARGALKQLADAEGYTRFVIPDDVGGRYSVLTAVGLLPIAAAGIDIDALMAGAAAAAAAYSDPDLERNPCYQYAAARNALYRKGMTTEVLVTYEPALKATAEWWKQLFGESEGKDGKGIFPAAAAFTTDLHSLGQYIQEGMRNLFETVVHVERPSVDIPLPPGDEGDGLGFLGGQTLHHVNHTAFLATRLAHTEGGVPNLTLTLPELTPYHYGYMLYFFMKACAISGYLLGVNPFDQPGVEAYKQNMYALLGKPGFEARRAEIEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR7</accession>
    <name>Q67QR7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39976">
        <property type="protein sequence ID" value="BAD39976"/>
        <property type="gene ID" value="STH991"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH991"/>
    <dbReference type="eggNOG" id="COG4911">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137908_1_0_9"/>
    <dbReference type="OMA" id="PAYLCWK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018699">
        <property type="entry name" value="DUF2203"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09969">
        <property type="entry name" value="DUF2203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016498">
        <property type="entry name" value="UCP016498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="18944" checksum="1B12F3588EFB17D7" modified="2004-10-11" version="1">MRVNRPRDGRGKVGMRMATRYFTPAEVNRLIPELERIIAYLRRLDAEIQEKDWRLRQRKVEARRRDDPVDTYTFLKEEAELDFLRILSRGQCDRIRELGGELKGGYLVDFPAIVDGQEVLLCWRPGEPEVRWYHGLHEGMMGRKPLPEAPGDEEARPEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R10</accession>
    <name>Q67R10_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion-translocating oxidoreductase complex subunit G</fullName>
            <ecNumber evidence="6">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rnf electron transport complex subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnfG</name>
        <name evidence="9" type="ordered locus">STH898</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RnfG family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195030.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH898"/>
    <dbReference type="EnsemblBacteria" id="BAD39883">
        <property type="protein sequence ID" value="BAD39883"/>
        <property type="gene ID" value="STH898"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH898"/>
    <dbReference type="eggNOG" id="COG4659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077882_2_1_9"/>
    <dbReference type="OMA" id="YSGAIHL"/>
    <dbReference type="OrthoDB" id="1381933at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00479">
        <property type="entry name" value="RsxG_RnfG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010209">
        <property type="entry name" value="Ion_transpt_RnfG/RsxG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36118">
        <property type="entry name" value="PTHR36118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006091">
        <property type="entry name" value="E_trnsport_RnfG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01947">
        <property type="entry name" value="rnfG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269944" description="Ion-translocating oxidoreductase complex subunit G" evidence="7">
        <location>
            <begin position="24"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="8">
        <location>
            <begin position="94"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="modified residue" description="FMN phosphoryl threonine" evidence="6">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="19102" checksum="B6F1D002E5D59176" modified="2004-10-11" version="1">MREAVRMILTLLVVTVLAGVALAGVNGVTAPIVAEMEARALQEGLAEVLPGAATFEEVPADELARAGGPVQAAWRGVDAEGEPVGVVVQAAPSGYGGAIVLLVGVEPGGTVHQLKVLSATGETPGLGSKATEPAFLQQFLGLSPSIELVKNQPPAGNEIQAVTGATISSRAVLDGVNAALDAARQLQTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R83</accession>
    <name>Q67R83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194958.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH825"/>
    <dbReference type="EnsemblBacteria" id="BAD39810">
        <property type="protein sequence ID" value="BAD39810"/>
        <property type="gene ID" value="STH825"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH825"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="IMMQKIM"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030660">
        <property type="entry name" value="ABC_branched_ATPase_LivF/BraG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039137">
        <property type="entry name" value="ABC_branched_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="239" mass="26027" checksum="E79A6FC8D59FEC8B" modified="2004-10-11" version="1">MILQVEDLYVAYGNIEALHGVSLEVNQGEIVTLIGANGAGKSTTLRAISGLNRAKSGTIRFQGRDITRTPAHEIVAMGIGHVPEGRRVFADLTVRENLELGAYLVHPSQFRKNLAEVYERFPRLKEREHQRASTLSGGEQQMLALGRALMLQPKLLLLDEPSMGLAPRLVQEIFEIIAELNRRGATILLVEQNAAMALSIAHRAYVLETGRVVLSGPASELRTHPAVREAYLGGAAHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R88</accession>
    <name>Q67R88_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA_16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194953.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH820"/>
    <dbReference type="EnsemblBacteria" id="BAD39805">
        <property type="protein sequence ID" value="BAD39805"/>
        <property type="gene ID" value="STH820"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH820"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_401066_0_0_9"/>
    <dbReference type="OMA" id="DAYQITH"/>
    <dbReference type="OrthoDB" id="332708at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_16" evidence="2">
        <location>
            <begin position="15"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="556"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="709" mass="79343" checksum="4FA6B154DE48E6B3" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN2</accession>
    <name>Q67RN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH676</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH676"/>
    <dbReference type="EnsemblBacteria" id="BAD39661">
        <property type="protein sequence ID" value="BAD39661"/>
        <property type="gene ID" value="STH676"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH676"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_27_9"/>
    <dbReference type="OMA" id="ARARTIM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="198"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="298"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="612" mass="65294" checksum="A93376EC20245911" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ3</accession>
    <name>Q67RQ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194788.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH655"/>
    <dbReference type="EnsemblBacteria" id="BAD39640">
        <property type="protein sequence ID" value="BAD39640"/>
        <property type="gene ID" value="STH655"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH655"/>
    <dbReference type="eggNOG" id="COG2014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076326_1_1_9"/>
    <dbReference type="OMA" id="TFTHEHE"/>
    <dbReference type="OrthoDB" id="1905652at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="DUF364" evidence="1">
        <location>
            <begin position="105"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="26951" checksum="230331775EABDE3B" modified="2004-10-11" version="1">MTLLERLYEAAAPRLCGKRVQDVRIGSHLVGVLLDSGEFGAAYALPGERQTPEPVFPDPARYVGMPAVELAAGLKQEDLVARALGMAAANAVARPEPGHCLEDPDALTTVPVRPADTVGLIGFIRSIHRRIEGRVNRIIVFDRSLDGEPGISPEEQQPELLPLCDVVFVTGSAVTNGTLEAVLPWCRRARDIVVIGPSTPLYPEAFAGTGVTVIAGATWPPEHHQAALAGIGEGKAFHDMHGLTRRWALRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR0</accession>
    <name>Q67RR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH648"/>
    <dbReference type="EnsemblBacteria" id="BAD39633">
        <property type="protein sequence ID" value="BAD39633"/>
        <property type="gene ID" value="STH648"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH648"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="EGSRNVY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="18"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="34911" checksum="CFDB1865A646D50F" modified="2004-10-11" version="1">MTEAEVRSARAAAPAVLIEARGLVKRYGDKLAVDGFSLQVRSGEIVGLLGPNGAGKTTAVEMMEGLRRPDAGQSFICGLDVHRQGRALRPRIGIALQAARLPEYATVLEILTLYASFYEDPMPVGDLLRQFSLEEKARTRFSHLSGGQRQRLALAVALIGRPKVLFLDEPTTGLDPQARHALWDVILDLKEEGRAILLTTHYMEEAERLCDRVAVMDHGRILAEGTPRELTRAYGPEAAVELDPGGARVDLAELARLSAVTGVRSEDGRVLLHTANPPATLMALARYAQERSLPLSDLRTRSATMDDVFMALTGRRLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT2</accession>
    <name>Q67RT2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ThrE_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH626</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194759.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH626"/>
    <dbReference type="EnsemblBacteria" id="BAD39611">
        <property type="protein sequence ID" value="BAD39611"/>
        <property type="gene ID" value="STH626"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH626"/>
    <dbReference type="eggNOG" id="COG3610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117642_3_0_9"/>
    <dbReference type="OMA" id="TNESFNY"/>
    <dbReference type="OrthoDB" id="1567942at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE_2" evidence="6">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="137" mass="14427" checksum="039724DB2979AC18" modified="2004-10-11" version="1">MITYPFAFLTSAAIAVSFRSPRGAILWTGLCGLVGWAGFDLALRAGAPAPAAVLLGAVTLGTASEVLARRLHQPAILFVIPGLFPLVPGIIAYRGMLLLSQSRLAEGAWQLARALAIAGILAAGLAIPPVLFRRWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV7</accession>
    <name>Q67RV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH601</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH601"/>
    <dbReference type="EnsemblBacteria" id="BAD39586">
        <property type="protein sequence ID" value="BAD39586"/>
        <property type="gene ID" value="STH601"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH601"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_635542_0_0_9"/>
    <dbReference type="OMA" id="QIADPFQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="405" mass="42058" checksum="B0D3CBE7ABBACC66" modified="2004-10-11" version="1">MGLAPGELDGRVAVVTGAARGIGHAAAAALAALGARVAVVDIAGEGEEAVEQIRAAGGEARFFGCDVSDEAAVAHLARAVRDGMGPASVVINNAVVCPVAPVEQMSAALWDRVFAVNLRGAFLVTRAFLPQLREAGTGTLVNLISTDAMPGLSAYIASKQGLVGFTQSLAAEIGDGSLRVVAFAPGMVETPGLLEAARGLAPLLGMSEAGFRRVSLHPAYEGLMPVEHAGLALAFLVARLAGEYHGQVASGYEVLERAGLITPCGPTAPAGEPGVGDVPLAEGLTRLKEILAETEADFQRLPVFVRPLARSGFRSRTGKPVQAWQQEADALLACVREGRPFDRDRLRADLDRLVAYVEGVPAETARFTRDPAVLAQVQARMDGYARDLRSLQAALTEGGRAAATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW0</accession>
    <name>Q67RW0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">GTP cyclohydrolase 1 type 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH598"/>
    <dbReference type="EnsemblBacteria" id="BAD39583">
        <property type="protein sequence ID" value="BAD39583"/>
        <property type="gene ID" value="STH598"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH598"/>
    <dbReference type="eggNOG" id="COG0327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037423_1_0_9"/>
    <dbReference type="OMA" id="EVAYDIY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017221">
        <property type="entry name" value="DUF34/NIF3_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037489">
        <property type="entry name" value="UCP037489_NIF3_YqfO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR037489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="368" mass="39370" checksum="3E9CCC27AFF627C2" modified="2004-10-11" version="1">MRNIAAYIEELAPLYWAESWDNVGLQVGDPGAAVQRVLVALELTDPVLEEAESLGCDMVVVHHPPIFRPLKALRFDNRPARRLLRLIRGGIALYAAHTNLDQAAGMTNDTLAAAAGLTEFEVLKQAGEERYVKLVVFVPRGHEDAVRDALAGAGAGHIGNYSHCTFQAPGTGTFLPLEGTNPFIGQQGRLERVEELRLETIVPESAAAQAVQAMIAAHPYEEVAYDLYPLANPGKVRGHGRIGRLAQPVSLAQLAERLKQALRTPAVRVVGDPERMVTTVAVGAGAGADLIRPAASRGADVLVTGDVKYHEAQDALDLGLALVDVGHYNSEAGAIGSLAAYLRERLAADGHPVEVVESQAGRDPFRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S39</accession>
    <name>Q67S39_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NfeD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH519"/>
    <dbReference type="EnsemblBacteria" id="BAD39504">
        <property type="protein sequence ID" value="BAD39504"/>
        <property type="gene ID" value="STH519"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH519"/>
    <dbReference type="eggNOG" id="COG1030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024619_2_0_9"/>
    <dbReference type="OMA" id="QMDTPGG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_sah"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="3">
        <location>
            <begin position="328"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="425" mass="44904" checksum="01E3E4896D089287" modified="2004-10-11" version="1">MTLAGAPGARAENGSGKVYVLRIDNGQVIDPGLAQFADRVFDEAGADPDAVAIAVVLDTPGGYVESAARIKDRLLHPPNGLKTIAYVENDAVSAGALIATAAEYLFMHPGAVIGAAEPRNALTGETADYKSLSVVVNYFTSAAEYRKRDPNIARAFVDKDHPMPWQTDVLLTLDYQKAVATGYANGVADSLHDAIRQAGIAQFQLVEPQWTFSEQVGRVLTTPWVAILLLVVGVIAFGIEFMQPGLTGPAALGIVCLTLFFLGNALVGTAGWVEIALAISGLVLLGVELFTPGFGIFGLAGIVAFGGAIFLAAPSAGLAWRYLTWTTVASAFVLFAVIRAISRRGLGKVLTLKDTAKDWSAQAEDRARLVGREGVSLTVLRPAGTAVFDDQRLDVVTEGEFIPAGVPVKIVRVEGTRIVVRAMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE5</accession>
    <name>Q67SE5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH413</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194547.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH413"/>
    <dbReference type="EnsemblBacteria" id="BAD39398">
        <property type="protein sequence ID" value="BAD39398"/>
        <property type="gene ID" value="STH413"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH413"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_0_9"/>
    <dbReference type="OMA" id="RAMAQNM"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="7">
        <location>
            <begin position="145"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="450" mass="47633" checksum="2B117E07E68FFF3A" modified="2004-10-11" version="1">MAYEFKLPDVGEGLHEAELLRWLVKEGDTVTEDQPIMEVQTDKATVEITSPVNGRVVKLLGQPGDILKVHSVVVIFDDGSPGALPTAGEVASGVAAAAPAGAQPQASLDVPAPAAQPAPAPAAPPAPAPAPAAGAGPADRPRRALATPATRRLARELGVDINQVPGTGPAGRVTSDDVRAFAARRTAPAPAQAPTQAPTEAAAPTPATPAPAAPAEADDERIPLRGIRKVIAERMVKSKYTAPHVTTVEEVDMTELMAFRAQAKELAARKGIKLSFMPFIIKAVVAALREFPYLNASIDDEAQEIVLHKRYHIGFALDTDAGLLVPVIKDADRKPVFAIAQEMNDLIARGREGKLAPDEMRGSTFTISNQGSIGGLFFTPVINYPEVAILGIGKTQPRPVVRDGEIVIRQMAHLALSFDHRLIDGGMATRFLNRLAELLSDPTLLMMEAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH1</accession>
    <name>Q67SH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194521.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH387"/>
    <dbReference type="EnsemblBacteria" id="BAD39372">
        <property type="protein sequence ID" value="BAD39372"/>
        <property type="gene ID" value="STH387"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH387"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_572264_0_0_9"/>
    <dbReference type="OMA" id="TWLACEL"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="477" mass="50461" checksum="A6A46B9CE34A2B14" modified="2004-10-11" version="1">MRLAALACTGDPLLDQLLGECASLALVGQPACERASDVLPLLTAEPPALIFVSAALADAEDLLKVRGPRFCWLAEGSDTDGGVPAPPGGVEVLRAEKLTTQLIDAWVRRLQKPPVRQVPPPAPVRPPLRPRSPSDGDSPETPDEPATAPGTRPRFPALSSPAARVPTPGGWPKMHALKSSPPAPSPPAGRAGPGTPSAPALRPPVRVIRQQVVALWGGKPGAGRSTMAVALADLLARSGDVRVCTVDLNPYNSSLSPLLGREQEVPSWVHLAEAAARSQPLPGSGLHWIRPNWALVSGPDGRPDLVARVTPEAIAWLVDELRSQFDYIILDPEARPGPVRDAAARLAQLVLVTVTCDYPDVLDTARGFEAAVEQGVLARDRCRLVLSRWLETPHLTQEEVAECFGLPVAAVIPLMPEAVLQAAGEARPVTRLSDPRAGRLVHALQQLLDVVAPALAAAGGDRSRSAPGSAWLDWLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ8</accession>
    <name>Q67SJ8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endopeptidase La</fullName>
            <ecNumber evidence="3">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH360"/>
    <dbReference type="EnsemblBacteria" id="BAD39345">
        <property type="protein sequence ID" value="BAD39345"/>
        <property type="gene ID" value="STH360"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH360"/>
    <dbReference type="eggNOG" id="COG1067">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020014_0_0_9"/>
    <dbReference type="OMA" id="GLYFWNL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008268">
        <property type="entry name" value="Peptidase_S16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014251">
        <property type="entry name" value="Spore_LonB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02902">
        <property type="entry name" value="spore_lonB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01046">
        <property type="entry name" value="LON_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="5">
        <location>
            <begin position="349"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="448"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="579" mass="62766" checksum="F8E276A6D3FB3B78" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL8</accession>
    <name>SSRP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">smpB</name>
        <name type="ordered locus">STH340</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SmpB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194474.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SL8"/>
    <dbReference type="SMR" id="Q67SL8"/>
    <dbReference type="STRING" id="292459.STH340"/>
    <dbReference type="EnsemblBacteria" id="BAD39325">
        <property type="protein sequence ID" value="BAD39325"/>
        <property type="gene ID" value="STH340"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH340"/>
    <dbReference type="eggNOG" id="COG0691">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108953_0_0_9"/>
    <dbReference type="OMA" id="WTNHSAR"/>
    <dbReference type="OrthoDB" id="1720952at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000103049" description="SsrA-binding protein">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <sequence length="158" mass="18341" checksum="FB06DB0B22D18F99" modified="2004-10-11" version="1">MARAKAEIQPVAENRKARHDYFVEETYEAGIVLVGSEVKSCRAGRVNLRDAYAQIKDGEIFLLNCHISPFEQANRFNHEPLRPRKLLMHKSEIHRLYGKVREKGFTLVPLRLYFNQKGKVKVELALAKGKRAYDKRDDIAAREAKREMARALRGRYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM6</accession>
    <name>Q67SM6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39317">
        <property type="protein sequence ID" value="BAD39317"/>
        <property type="gene ID" value="STH332"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH332"/>
    <dbReference type="HOGENOM" id="CLU_1651283_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17634" checksum="B4CE07B53C62CA1B" modified="2004-10-11" version="1">MGFWGRRKERLAPLVLTTAPCRVNGTVCCSWQGACSGTNPTRRSRIRRFPYPLIQKHACIFFGYLPYITSGIRADKGGLSPVIPPITCDKGYPCVQTQGSTKRRAPAGLIGGGCFVFQGLHCQVVGIPQRASMRCRKSAYSGVLLSFRRRRRKNSLVVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN8</accession>
    <name>Q67SN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH320"/>
    <dbReference type="EnsemblBacteria" id="BAD39305">
        <property type="protein sequence ID" value="BAD39305"/>
        <property type="gene ID" value="STH320"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH320"/>
    <dbReference type="eggNOG" id="COG0695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2653157_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="76" mass="8434" checksum="569003DA16F96727" modified="2004-10-11" version="1">MYTKESCPGCLQIKRMLNYLQLAYEERRVDEQSADRERILALGFRSLPVVEVEGVGAVPGADPAQVEDLLVRAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP7</accession>
    <name>Q67SP7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194446.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH311"/>
    <dbReference type="EnsemblBacteria" id="BAD39296">
        <property type="protein sequence ID" value="BAD39296"/>
        <property type="gene ID" value="STH311"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH311"/>
    <dbReference type="eggNOG" id="ENOG502Z7WH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031037_1_0_9"/>
    <dbReference type="OMA" id="TIRDRFY"/>
    <dbReference type="OrthoDB" id="924061at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010144">
        <property type="entry name" value="CRISPR-assoc_prot_Csd1-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09709">
        <property type="entry name" value="Cas_Csd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01863">
        <property type="entry name" value="cas_Csd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="591" mass="65628" checksum="B9BFA8D48C0351BF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS1</accession>
    <name>Q67SS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Group II intron-encoding maturase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH287"/>
    <dbReference type="EnsemblBacteria" id="BAD39272">
        <property type="protein sequence ID" value="BAD39272"/>
        <property type="gene ID" value="STH287"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH287"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2467871_0_0_9"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIIM" evidence="2">
        <location>
            <begin position="7"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="10810" checksum="A1333D9FD7B3926C" modified="2004-10-11" version="1">MADKAHKRVKRELRRMTKRSRSQSMKERIQRINAYLRGWIGYYALSDADSVFKEIEGWLRHRLRACLWKQWKRPRTRLRELRALGLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS5</accession>
    <name>Q67SS5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gly-zipper_Omp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194418.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH283"/>
    <dbReference type="EnsemblBacteria" id="BAD39268">
        <property type="protein sequence ID" value="BAD39268"/>
        <property type="gene ID" value="STH283"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH283"/>
    <dbReference type="eggNOG" id="ENOG5032VM8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590418_0_0_9"/>
    <dbReference type="OrthoDB" id="1322593at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039567">
        <property type="entry name" value="Gly-zipper"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13488">
        <property type="entry name" value="Gly-zipper_Omp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gly-zipper_Omp" evidence="2">
        <location>
            <begin position="168"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="463" mass="51356" checksum="06638A9656C0AB63" modified="2004-10-11" version="1">MKLELDPEAYAGWFERLKGYVGEVVAADVLARQGYQVELADVPNQPGWDLLVEGQPFNVKITDDPDQILEHFAEYPDIPVITSPEVAAELPPELQDHVLALEGLEADQIAELTQETLDGVEGLDIGPSFPVVPAALSALREIQHLALGQTDLGTSLRSAALDIAGTGIGGWVGAKVGAAVGTAILPGLGTVIGALLAGLAGSMVGRAITNDIKLAALRKALEEYQEQYARFHKTATEEHNILVAKVVRTIDIHRAELLAEIQAIRRRHEEAARQARNRFWDVAQDLGKRLPELLLRAEAELAEEQQRVLAELPRSPVWRRILWPSADDLRYRAVELWFTRQRQELRRMASEFSKNLDKLTPEGMLAEVRKCLSGKIFDSAALKEAVDRVVSEYNATRARIALERRLAVQEVRAAVGKRAAELHRYTAEQVKTLTQRVRPLAEALRPYREKVEAEAAKLGYQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU0</accession>
    <name>Q67SU0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194403.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH268"/>
    <dbReference type="EnsemblBacteria" id="BAD39253">
        <property type="protein sequence ID" value="BAD39253"/>
        <property type="gene ID" value="STH268"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH268"/>
    <dbReference type="HOGENOM" id="CLU_1209286_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25322" checksum="D1CA3EEEC0CED655" modified="2004-10-11" version="1">MITRIEYVVCPTRLVPDAPYISQLASADALADLIRKELRAAYPAAEVVVRVARLMEMLAPDACLQVVAEDGCDQDARLAVEQIIQSVCTDRQAEWIIIDRTEPMLQSDVAAIAENILRCHLGRLHPPGTIHSTVREWASRIISDPSYAETLAEVGGEVPYHQLTQYQAGITALVSDLCHDLVRFRRGEATDLAPAIIWAYERHLSVLTDPPAPSPSAAWHLPSPSEKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU4</accession>
    <name>Q67SU4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39249">
        <property type="protein sequence ID" value="BAD39249"/>
        <property type="gene ID" value="STH264"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH264"/>
    <dbReference type="HOGENOM" id="CLU_2620756_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8651" checksum="48BF398AE57B1C3B" modified="2004-10-11" version="1">MRPVCLGSVNPCRPPALRECLAAPGEECTKISGAGELQLDGTSRSIPEHEYVTWVRPAVRLTIEVDLNRKLRQHGRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU7</accession>
    <name>Q67SU7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gate domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194396.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH261"/>
    <dbReference type="EnsemblBacteria" id="BAD39246">
        <property type="protein sequence ID" value="BAD39246"/>
        <property type="gene ID" value="STH261"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH261"/>
    <dbReference type="eggNOG" id="COG3314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1905707_0_0_9"/>
    <dbReference type="OrthoDB" id="819609at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="42"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="133" mass="14508" checksum="3F010028EC29EEAA" modified="2004-10-11" version="1">MRGKFTTPVLAAVAVMLTAALVIYPKESLEAAKEGMNLFFTVVFPSLLPFFILSEMLLGLGVVHFIGVLFTPLMRPLFNVPGEGAFVLSMGLAAGYPMDAVITARFRRSNMCTRVEGERMLAFSNTADPVFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T13</accession>
    <name>Q67T13_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Germane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH195"/>
    <dbReference type="EnsemblBacteria" id="BAD39180">
        <property type="protein sequence ID" value="BAD39180"/>
        <property type="gene ID" value="STH195"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH195"/>
    <dbReference type="eggNOG" id="COG5401">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1098064_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019606">
        <property type="entry name" value="GerMN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10646">
        <property type="entry name" value="Germane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00909">
        <property type="entry name" value="Germane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Germane" evidence="2">
        <location>
            <begin position="138"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="25881" checksum="ABDB5537C1C480AE" modified="2004-10-11" version="1">MQLQAEAEPEPAVGQAAEGAADRAAQAGGDRLVEVDEEQAAEAGGEQVADAAADQAAEADAADAAAAGPDAGVGPGPAPEPGSAGEPVYAPATSRSAIDRSVGAAETMRVTVYYVDFDAHMEALQPVQILVPFSVTPIKVSVEQLLRPPEELGLFSEFPPGTTARGFNLVDGVAVVDLSATVEGVRGETAVNAVVATLVYTLTEIPGVEAVQLWADGKPAVLDGFTWSSPLTRADVAAWQAFRVEPVIEYSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T31</accession>
    <name>Q67T31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH177"/>
    <dbReference type="EnsemblBacteria" id="BAD39162">
        <property type="protein sequence ID" value="BAD39162"/>
        <property type="gene ID" value="STH177"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH177"/>
    <dbReference type="eggNOG" id="COG1950">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120441_2_4_9"/>
    <dbReference type="OMA" id="VNGLCFW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007165">
        <property type="entry name" value="Phage_holin_4_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37309">
        <property type="entry name" value="PTHR37309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04020">
        <property type="entry name" value="Phage_holin_4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="124" mass="13065" checksum="D9C7BB30E669C7C8" modified="2004-10-11" version="1">MMLRLLVRWALGALTLLAIAYVAPSLGILPGFHVAGFGAALVAVVVLSLLNLTVRPILKWLSLPITCLTFGLFTLVINAVVMLLTDALVAGFEVGGFLNAVIVSVIYAVVTALLYGLVEGDQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T78</accession>
    <name>Q67T78_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194265.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH130"/>
    <dbReference type="EnsemblBacteria" id="BAD39115">
        <property type="protein sequence ID" value="BAD39115"/>
        <property type="gene ID" value="STH130"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH130"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_9"/>
    <dbReference type="OMA" id="FATWGRI"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="100"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="302" mass="32372" checksum="A188CE02D671616B" modified="2004-10-11" version="1">MAATAEVRAPAAAGAEPRSRTAWDRLRQHKLAMAGLIFIVLLILMAILAPWLAPADPNKQDLFLKLAKPGTPFDALGEGKYWLGADHLGRDVFSRVIYGARVSLLVGFVAEAIVMVIGVTMGALAGYYGGRVDRVIMRTVDVLFAFPDLLFAIAIMFALGRGLINLFIALSVVGWAGMARLTRGQVLALKEREFVEGARAAGASDARVILRHILPNALGPIIVAVTLGIPGAIMSEASLSYLGLGVQPPTATWGSMIYEGRSYLRHAPWMSLAPGVMIMLTVLAFNLLGDGLRDALDPRTKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC9</accession>
    <name>UPP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">upp</name>
        <name type="ordered locus">STH79</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194214.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TC9"/>
    <dbReference type="SMR" id="Q67TC9"/>
    <dbReference type="STRING" id="292459.STH79"/>
    <dbReference type="EnsemblBacteria" id="BAD39064">
        <property type="protein sequence ID" value="BAD39064"/>
        <property type="gene ID" value="STH79"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH79"/>
    <dbReference type="eggNOG" id="COG0035">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_2_9"/>
    <dbReference type="OMA" id="TYATRMP"/>
    <dbReference type="OrthoDB" id="1581906at2"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_B">
        <property type="entry name" value="Upp_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034332">
        <property type="entry name" value="Upp_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120901" description="Uracil phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <sequence length="209" mass="23042" checksum="C68E134F84CC4D53" modified="2004-10-11" version="1">MSRVVVIDHPLIQHKLSIIRDKDTGPKEFRELVNEIAMLMAYEVTRDLPTEEVEVDTPIARARCRRLAGEKLGLIPILRAGLGMVQGILSLYPTARVGHIGLYRDPDTLKPVEYYCKLPTDLGERELLVLDPMLATGGSVVASLDLIKRQGGRRIKLLCLIAAPEGVQAVQEAHPDVDIYLAALDEMLNEHAYIVPGLGDAGDRLFGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK2</accession>
    <name>Q67TK2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA gyrase subunit B</fullName>
            <ecNumber evidence="10">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">gyrB</name>
        <name evidence="12" type="ordered locus">STH6</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194141.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH6"/>
    <dbReference type="EnsemblBacteria" id="BAD38991">
        <property type="protein sequence ID" value="BAD38991"/>
        <property type="gene ID" value="STH6"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH6"/>
    <dbReference type="eggNOG" id="COG0187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006146_1_2_9"/>
    <dbReference type="OMA" id="LWETTMH"/>
    <dbReference type="OrthoDB" id="205481at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
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        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="421"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD38991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="638" mass="71238" checksum="F24D1BA0B6BFB446" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J31</accession>
    <name>Q67J31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SpoIIIJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoIIIJ</name>
        <name evidence="8" type="ordered locus">STH3338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3338"/>
    <dbReference type="EnsemblBacteria" id="BAD42319">
        <property type="protein sequence ID" value="BAD42319"/>
        <property type="gene ID" value="STH3338"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3338"/>
    <dbReference type="eggNOG" id="COG0706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036138_4_1_9"/>
    <dbReference type="OMA" id="QWPILIA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01900">
        <property type="entry name" value="YIDCPROTEIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="28428" checksum="0B81B93C2FD9C2EF" modified="2004-10-11" version="1">MPPLSSGRVRPGPLAADIPNSPGGFPYLITSFLVPEWLVQPMTKLLEVFLKWTGNYGLAIILLTVVVRIVILPLTVYQMRSMKRMQEVQPLMKELQDKYKDNPEKLNQELMALYQREKVNPFSGCLPLLVQLPILYALFAVFNAFDPTKFNYTYQFLWADLSQRDIPLAILTVVSMILQQYLTGMPTTDPNQRVMMWIMPIMFGWIAFTMPTSSIVLYWVVSTFFGLIQQAIYPGFPRFKGSLGSKGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J43</accession>
    <name>Q67J43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3326"/>
    <dbReference type="EnsemblBacteria" id="BAD42307">
        <property type="protein sequence ID" value="BAD42307"/>
        <property type="gene ID" value="STH3326"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3326"/>
    <dbReference type="eggNOG" id="COG1664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072799_6_5_9"/>
    <dbReference type="OMA" id="LDYHLHI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007607">
        <property type="entry name" value="BacA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35024">
        <property type="entry name" value="PTHR35024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04519">
        <property type="entry name" value="Bactofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="137" mass="14488" checksum="7315893EF540CD82" modified="2004-10-11" version="1">MTVFGSRKKSTADNLSHGKIETLIGEGTQIKGTFQSSGVVRVDGFLEGSIEHDGDLIVGPKGRIQATIKARSLATAGEIHGDVEVAEKLELLPGAHLKGDVRCGHLVIHEGARFHGRSLMSVTDSAEPPQPDRSPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE4</accession>
    <name>Q67JE4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative site-specific modification DNA-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3224"/>
    <dbReference type="REBASE" id="10275">
        <property type="entry name" value="M.StpORF3224P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42206">
        <property type="protein sequence ID" value="BAD42206"/>
        <property type="gene ID" value="STH3224"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3224"/>
    <dbReference type="eggNOG" id="COG0827">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020255_1_0_9"/>
    <dbReference type="OMA" id="AYKCRVR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="538" mass="59709" checksum="C2FE705CF6F71AB5" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF7</accession>
    <name>Q67JF7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3211</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197324.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3211"/>
    <dbReference type="EnsemblBacteria" id="BAD42193">
        <property type="protein sequence ID" value="BAD42193"/>
        <property type="gene ID" value="STH3211"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3211"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022800_3_0_9"/>
    <dbReference type="OMA" id="FIVQGSM"/>
    <dbReference type="OrthoDB" id="366413at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027022">
        <property type="entry name" value="ABC_permease_BceB-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018968">
        <property type="entry name" value="ABC_permease_BceB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="608"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="86"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="643" mass="71425" checksum="CFBEF81A6E37BD98" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN6</accession>
    <name>Q67JN6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3132"/>
    <dbReference type="EnsemblBacteria" id="BAD42114">
        <property type="protein sequence ID" value="BAD42114"/>
        <property type="gene ID" value="STH3132"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3132"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064908_0_0_9"/>
    <dbReference type="OMA" id="FLGCPWR"/>
    <dbReference type="OrthoDB" id="1104816at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026366">
        <property type="entry name" value="Seleno_YedE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04112">
        <property type="entry name" value="seleno_YedE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="223"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="375" mass="38823" checksum="E9616C050AA82C75" modified="2004-10-11" version="1">MSLNRLWSGRWGVTLTGVVVGILAPILVKLGNPGNMGVCVACFTRDIAGALGLHRAAAVQYIRPEIIGLVLGATIAALGAGEWRARGGSSPLVRYIMGVIAAIGALVFLGCPWRAFLRIGGGDWNAVVGVLGFAAGIWAATFFLRRGYSLGKSKPMAPVAGWIMPAAMVGLLVLLLAAPLFGRDADGNPVGPIFFSTSGPGSQHAAWWISLLFALVIGYLGQRSRFCSVGAFRNLFMLRDTKLLTGVVALIITAAVANLALGQFHPGFANQPIAHTDGLWNFMGMLLSGLAFTLGGGCPGRQLFLSGEGDTDAAVFTFGLLTGTALAHNFNLASSAAGPSPYGPAAVIIGLIVVVVLGFTMRDVAAGEDQSRSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K40</accession>
    <name>Q67K40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41958">
        <property type="protein sequence ID" value="BAD41958"/>
        <property type="gene ID" value="STH2975"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2975"/>
    <dbReference type="HOGENOM" id="CLU_2132276_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="13340" checksum="3AE821C0C166E861" modified="2004-10-11" version="1">MPRFHDSPCTASRRPCLRRGIRNPRQLSHEPAHLGVERLLLLSKGLKLLTKSDFVPWQEQRNRRLHRRIPATNEFVHLVHRRRGRGLLQGLQELPTRFVRPLVRLVEALLPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K81</accession>
    <name>Q67K81_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mRNA interferase</fullName>
            <ecNumber evidence="3">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2934</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Toxic component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PemK/MazF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197050.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2934"/>
    <dbReference type="EnsemblBacteria" id="BAD41917">
        <property type="protein sequence ID" value="BAD41917"/>
        <property type="gene ID" value="STH2934"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2934"/>
    <dbReference type="eggNOG" id="COG2337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121823_1_0_9"/>
    <dbReference type="OMA" id="NDIGNQY"/>
    <dbReference type="OrthoDB" id="1754531at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003477">
        <property type="entry name" value="PemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011067">
        <property type="entry name" value="Plasmid_toxin/cell-grow_inhib"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33988">
        <property type="entry name" value="PTHR33988"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02452">
        <property type="entry name" value="PemK_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033490">
        <property type="entry name" value="MazF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR033490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="120" mass="13472" checksum="A31CBB68C9EB8D48" modified="2004-10-11" version="1">MSKPLQIRRGDVFYADLSPVIGSEQGGVRPVLILQNDVGNKYSPTVIVSAITSQIEKAKLPIHVELDGETYGLERDSVILLEQVRTIDKRRLRERVTHLGSDIMRQVDEALMISLGLKEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K93</accession>
    <name>RNH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease H</fullName>
            <shortName evidence="1">RNase H</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhA</name>
        <name type="ordered locus">STH2922</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197039.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67K93"/>
    <dbReference type="SMR" id="Q67K93"/>
    <dbReference type="STRING" id="292459.STH2922"/>
    <dbReference type="EnsemblBacteria" id="BAD41905">
        <property type="protein sequence ID" value="BAD41905"/>
        <property type="gene ID" value="STH2922"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2922"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_6_2_9"/>
    <dbReference type="OMA" id="MQEIEIF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000332680" description="Ribonuclease H">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00408"/>
        </source>
    </evidence>
    <sequence length="147" mass="16584" checksum="221D1CCA7D5800A0" modified="2004-10-11" version="1">MREVIIYTDGACSGNPGPGGWGAVLLYGSHRKELSGFHPHTTNNRMEIQAAIEALRALKYPCKVKLYSDSAYLVNAFRQNWLRTWQRNGWVNSRKQPVENQDLWQELLEAARPHQVEWLKVQGHADVAENNRCDELARAAIAAGTQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF4</accession>
    <name>Q67KF4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2859"/>
    <dbReference type="EnsemblBacteria" id="BAD41844">
        <property type="protein sequence ID" value="BAD41844"/>
        <property type="gene ID" value="STH2859"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2859"/>
    <dbReference type="HOGENOM" id="CLU_3030779_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="55" mass="5873" checksum="CDB97CC25545DDAB" modified="2004-10-11" version="1">MTSLGSIIVGALGGLIVTVVVMYFWPRSRNRHLGALLAAVLGVAFAFVVMTQVRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH7</accession>
    <name>HIS7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="1">IGPD</shortName>
            <ecNumber evidence="1">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisB</name>
        <name type="ordered locus">STH2836</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196955.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KH7"/>
    <dbReference type="SMR" id="Q67KH7"/>
    <dbReference type="STRING" id="292459.STH2836"/>
    <dbReference type="EnsemblBacteria" id="BAD41821">
        <property type="protein sequence ID" value="BAD41821"/>
        <property type="gene ID" value="STH2836"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2836"/>
    <dbReference type="eggNOG" id="COG0131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_9"/>
    <dbReference type="OMA" id="ARHGLFD"/>
    <dbReference type="OrthoDB" id="1278103at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158176" description="Imidazoleglycerol-phosphate dehydratase">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <sequence length="208" mass="22605" checksum="E5D69EAA4339B77C" modified="2004-10-11" version="1">MTDCSRNETGALAERVGQVARKTRETDIAVTWRLDGAGRADVDTGVPFFDHMLDQIARHSLTDLTARAVGDLEIDAHHTVEDTGIALGQALRRALGDGRSIRRYGSAFVPFDETLAFAAVDVSGRPYLVFDAALPAQKVGNFDTELAEEFFRALAMNAGITLHLKVHYGRNTHHMIEGLFKAFARALGDAVARDPRVLGVPSTKGALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL9</accession>
    <name>Q67KL9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196913.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2794"/>
    <dbReference type="EnsemblBacteria" id="BAD41779">
        <property type="protein sequence ID" value="BAD41779"/>
        <property type="gene ID" value="STH2794"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2794"/>
    <dbReference type="HOGENOM" id="CLU_1495466_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="19266" checksum="A4FA828A2A364674" modified="2004-10-11" version="1">MNAKGCWDDMTLDLFRMGLLEEAEAEAVRGHVQRCAACQVRLGALDLEDLRLEAALALTREEAKALAAADLPGRLAARVSVGRNGARALAVGLSGLGLAAVAGLQGAVLPWLQGLPLGGWMTAGHWAPELAGRLLRSARSFLFAPPTSRHNVLVPLLAAGLVIILLNLRHRRRRLSPFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM0</accession>
    <name>Q67KM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2793"/>
    <dbReference type="EnsemblBacteria" id="BAD41778">
        <property type="protein sequence ID" value="BAD41778"/>
        <property type="gene ID" value="STH2793"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2793"/>
    <dbReference type="eggNOG" id="COG1664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_700052_0_0_9"/>
    <dbReference type="OMA" id="ADRENRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269518" evidence="2">
        <location>
            <begin position="30"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="394" mass="40642" checksum="2C98882F250B03CA" modified="2004-10-11" version="1">MNRMRRWRIWLVLTLAALAAAVSTVGAAAAGGPEAGRPPIAAGEEQVIIPEGERLEDDLVVNAEKGAAVYGTLAGDLILLGADAVITGTVEGDVIGYANRVWISGQVLGNVRVLALEAVTIEGRVGRSAITVSQRATLGPEAEIGTTWFALSGDVELGGTVGRSLFATASRVTVSGEVGGDLSLHGYDQARVLPGAVVRGGILAVADRPPVVDDGASVGEVRFVAREGATQPLLTMDGFALGRLAGFAAVGLLVTWLAPGLLGSFQRRVSGHFWATLAVGAGLLAGVPVLALVLMLTVGGIPAALMVVLPLYAAAIYLGQVFVAGWLGWAILGRVRGDGQAPRSAAFLLGLVCLTLFSRLPYVRYVGSFLAVSLALGGLSLTLGPWIRRIARED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN1</accession>
    <name>Q67KN1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine sulfhydrylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2782"/>
    <dbReference type="EnsemblBacteria" id="BAD41767">
        <property type="protein sequence ID" value="BAD41767"/>
        <property type="gene ID" value="STH2782"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2782"/>
    <dbReference type="eggNOG" id="COG2873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_2_9"/>
    <dbReference type="OMA" id="TYTLFAH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="426" mass="46387" checksum="58E8ECF363392E47" modified="2004-10-11" version="1">MAERKYRLETLAIHAGHEIDPTGARAVPLYMTTSYVFRDTAHAARLFSLQEPGNIYTRIMNPTTDVFEKRMAALEGGVGALAVASGQNAELYAILNLARAGDEIVAATSLYGGTYALFAHNLPRLGITVKFVEPSDPENFRAAITDRTRALYIETIGNPRCDVPDIAAIAAVAHEHGLPLIVDNTFASPYLCRPIEHGADIVIHSATKFIGGHGTAIGGVIVDGGRFDWGAGRHPLYTEPDPAYHGLRYADLGEQAYILRARVSLLRDMGGALSPFNAFLFLQGLETLHLRMERHSANALAVARFLEAHPKVEWVTYPGLESSPYYGNAKRYLPRGQGAILAFGIRGGLEAGRRFIESLRLFSHLANVGDSKSLAIHPASTTHEQLSDEERRAAGVTPEMVRLSVGTEHIDDIIEDVAQALERATN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP1</accession>
    <name>NUOI2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit I 2</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit I 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit I 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoI2</name>
        <name type="ordered locus">STH2772</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011196891.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KP1"/>
    <dbReference type="STRING" id="292459.STH2772"/>
    <dbReference type="EnsemblBacteria" id="BAD41757">
        <property type="protein sequence ID" value="BAD41757"/>
        <property type="gene ID" value="STH2772"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2772"/>
    <dbReference type="eggNOG" id="COG1143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1155919_0_0_9"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="PTHR10849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000245753" description="NADH-quinone oxidoreductase subunit I 2">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="1">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="1">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="240" mass="26924" checksum="1C46A571E9A0D671" modified="2004-10-11" version="1">MGVTNFFGEAWRTGKSIVTGLGITFREMMFRPAITVFYPEQRDDVPPWFRGIPVLKTDLRTGEYKCTSCMQCAQACPVNVITIEWHQDPETKKKVCDRFAIDMSRCMLCNFCVEACPFDSLVMGYDYELCKVNPENLVFEFEDLLRLGLKYSKAEEPGPKATRSSTPPWVFHGLTNATEDDIQDIHGYLGRPPLPKGYEPELKPQFRKPAEEAAEAQQAEAAGQPAAEPGKTNGEEAGQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-02-09" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP6</accession>
    <name>NUON2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit N 2</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit N 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit N 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoN2</name>
        <name type="ordered locus">STH2767</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KP6"/>
    <dbReference type="SMR" id="Q67KP6"/>
    <dbReference type="STRING" id="292459.STH2767"/>
    <dbReference type="EnsemblBacteria" id="BAD41752">
        <property type="protein sequence ID" value="BAD41752"/>
        <property type="gene ID" value="STH2767"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2767"/>
    <dbReference type="eggNOG" id="COG1007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_5_9"/>
    <dbReference type="OMA" id="FYIRVIV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000391234" description="NADH-quinone oxidoreductase subunit N 2">
        <location>
            <begin position="1"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00445"/>
        </source>
    </evidence>
    <sequence length="508" mass="53747" checksum="3526A2DB4CCD79A4" modified="2004-10-11" version="1">MPGVVSWSQLVNPSYVAILPHLIVTATLLVVIVLDAYFKEKRSLVWVTLGGVVLAMLSIWYTASDPQIQAGIAAGRPPEFWGGMIIADGFTFFMNGVLLGIAALVILLSADYVGKFLRGAHMEFYEIILAVTLGMMFMVSSRDLLTIYIGLELTSISSYVLAGILRKDAKSNEAALKYFLTGATASAVLLFGLSLIYGVTGSTRLPEVAAALAGGSHVVAAAGPALTPLLVAGMAFLMVGFGFKVAAVPVHQWAPDVYEGAPTPVTAFFSAGPKGAAMAAILRVFVGGLGVAPFTDKWALIWALAAAASMTVGNLVALQQTNIKRMMAYSSIAQAGYILVGVAASGLQSVEGISSVLFYVMAYAVTNLGIFAVLTHMDQEGGWVEVDNYAGLAKRNPLYAWALLLFFVSLIGIPPTVGFLGKFFLFRAAAASGYLWLAVLMAVNSVISVGYYYRVVKVMFLDQSDYPALTPSTGISATVLLSLLGVVALTIFANPFVQWTAQSAALLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ8</accession>
    <name>Q67KQ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class I aminotransferase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2754</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196873.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2754"/>
    <dbReference type="EnsemblBacteria" id="BAD41739">
        <property type="protein sequence ID" value="BAD41739"/>
        <property type="gene ID" value="STH2754"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2754"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_0_9"/>
    <dbReference type="OMA" id="CDYVYVT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="489" mass="53387" checksum="E42DEC76C1E1A49E" modified="2004-10-11" version="1">MFKLDRTSATPLYRQLYAQAKERILSGALPPGTRLPPERTLARRLGVNRTTVVNAYRELAAEGLVEGRVGHGTVVLGPPEPRGADAVRPMPWAGPTEAEEPLVADINAIIRRPGAISFAHGEMSPELYPAEAVAHLMQQVLQDPAALGYGPIAGLMPLRQAIADAMGVTADQVLILAGSQIALYLISRVLLQPGDTVLVEMPSYINTLGIFDSAGVRVVPVPVDQHGLVVDGLDELMLRYQPKLLFTLPTFHNPLGVTLTPERRRRLIQLAARHQVGIVEDDPYGPLHFTGQPVPTLKSLDPGGYVIYISSASKAVSPALRLAWIVAAPPVVERIARARGSLDFRAALLNQRVMEAFLREGLLAEHLGRLRPALKERRDLMLEALQRYMPDGVSWYVPEGGYHIWCRLPRPLTARRLLTEAGREGVAFVPGDFYGAGQMARRGLRLNFSYPRPEEIGPGIARLARAVERLLREEAVDGGEEASISGPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF3</accession>
    <name>Q67LF3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative potassium channel beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196631.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2508"/>
    <dbReference type="EnsemblBacteria" id="BAD41493">
        <property type="protein sequence ID" value="BAD41493"/>
        <property type="gene ID" value="STH2508"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2508"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_9"/>
    <dbReference type="OMA" id="EFAIAWV"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="PTHR43150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="2">
        <location>
            <begin position="15"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="36296" checksum="6B58C99E911F1403" modified="2004-10-11" version="1">MEYRRLGSSGLKLSEISLGSWLTYGSRVEAEAAKACIRTAYEHGINHFDCAVVYGAQPHQAEEVVGEALREFPRHTYTVTSKVFWKVGPSTYDQGLSRKHLIERVEMSLKAMKLDYIDIYYAHRFDPETDLEETLRAFDDLVAQGKILYWGFSQWTPAQIERAAAIVDKRNLRRPVVSQPVYNMIDRGIEQEVMPICAREGIGQVVYSPLAQGVLTGKYKPGMPIPEGSRGADPKYGKFVQRYLSDEEMLRRIQQLQPIAERNGLTMAQLALAWVLRRPEVTSAIIGATRPEQVVENVKASGVKLSEEDLKEIDRILGYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG9</accession>
    <name>Q67LG9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative S-layer associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2492</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2492"/>
    <dbReference type="EnsemblBacteria" id="BAD41477">
        <property type="protein sequence ID" value="BAD41477"/>
        <property type="gene ID" value="STH2492"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2492"/>
    <dbReference type="eggNOG" id="COG2340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_794194_0_0_9"/>
    <dbReference type="OMA" id="IMITRAL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014258">
        <property type="entry name" value="CAP_domain_YkwD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02909">
        <property type="entry name" value="spore_YkwD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="83"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="148"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="207"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="410" mass="43655" checksum="352933805ECF6669" modified="2004-10-11" version="1">MGCDRLCSYGNLTVCSQKLTHFDRLGGAPVSQRPFPHHLAALAIAATLLGILPVSASGLRAILPGAVAPGRVLPGAAPALAAPAAYTDQAQIPAWAAADIEFTTELGIFRGDADTGAFRPHDQITRAEVAAVVLRAFPLAADPAVRAPFVDIHGGWYEEPVTRAVQAGVIQAGDFGPWFRPNQPITRAELARMLARAIGVCHVSPSSVAFSDVDPNQAPFGPYIARAAGCGIIRGMGDGTFAPDQTATRAQAAVMIARALRLTQAGSAPPQPAEPAPAGGPSAEDGIHEFERRVAELVNAERLAAGLQPLRLDPGLSRVARLKSRDFVTGGYFGHESPTYGSPFEMMKQFGFTYRMAGENIALGHRTPEQVHEAWMNSPGHRSNILNPDFDTIGVGYYEYGWTQMFIQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH6</accession>
    <name>Q67LH6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenylacetic acid degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaB</name>
        <name evidence="1" type="ordered locus">STH2485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196608.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2485"/>
    <dbReference type="EnsemblBacteria" id="BAD41470">
        <property type="protein sequence ID" value="BAD41470"/>
        <property type="gene ID" value="STH2485"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2485"/>
    <dbReference type="eggNOG" id="COG3460">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166737_0_0_9"/>
    <dbReference type="OMA" id="VYEIFVQ"/>
    <dbReference type="OrthoDB" id="2083684at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009359">
        <property type="entry name" value="PaaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038693">
        <property type="entry name" value="PaaB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06243">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030200">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="13760" checksum="7ABA38BC56CEA81B" modified="2004-10-11" version="1">MARPPGEGGRRFRVYEIFVQKDHQSPHVWVGSVDAGSAEDALLLARESFLRRDPAVSIWAVEQSAIHRTDYADRDFFARETDKRYRTLAGYRESNAERWRKYRRELLDLERDFVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ8</accession>
    <name>Q67LJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase, short-chain specific</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2463</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196586.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2463"/>
    <dbReference type="EnsemblBacteria" id="BAD41448">
        <property type="protein sequence ID" value="BAD41448"/>
        <property type="gene ID" value="STH2463"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2463"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_9"/>
    <dbReference type="OMA" id="FQGLRFM"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="8">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="121"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="230"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="381" mass="42341" checksum="E26DE8840AE30E64" modified="2004-10-11" version="1">MNFFLTEEQNMVRQAIRELAEKEIAPRSAEFNRKHEFPWPNFKLLAENGYLNMHLPEPYGGGMDWVSYAIVIEELARACAVTAVIYEVHCSLHSEAIFHFGSEEQKQKYLPRLTRGEILGAYALTEPGAGSDAAAIRTTAIRDGDSYILNGEKTFITNGGQAGLYVVFARTNPDPSVGHRGISAFLVEADTPGFSVGKPMEKMGLHASHTTTLHFEDCRIPASQLLGKEGEGFKIAMAILDRGRIGIAAQAVGITQAALEDSIKFAKERHTFGKPIAEHQAIAWKIADMATDLEAARLLLYRAAFLMQQGVRATKEISMAKLFASEMAMKHTVEAVQIHGGYGYMQEYRVERLMREAKITQLYEGTSEIQRLVISRALLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LM4</accession>
    <name>Q67LM4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2437</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate</text>
            <dbReference type="Rhea" id="RHEA:22088"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:142410"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the relA/spoT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.5" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2437"/>
    <dbReference type="EnsemblBacteria" id="BAD41422">
        <property type="protein sequence ID" value="BAD41422"/>
        <property type="gene ID" value="STH2437"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2437"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012300_3_0_9"/>
    <dbReference type="OMA" id="DWISSPK"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008728">
        <property type="term" value="F:GTP diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045600">
        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="6">
        <location>
            <begin position="48"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="387"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="5">
        <location>
            <begin position="649"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="735" mass="83539" checksum="812479177D9A6F50" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP0</accession>
    <name>SYDND_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">STH2421</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LP0"/>
    <dbReference type="SMR" id="Q67LP0"/>
    <dbReference type="STRING" id="292459.STH2421"/>
    <dbReference type="PRIDE" id="Q67LP0"/>
    <dbReference type="EnsemblBacteria" id="BAD41406">
        <property type="protein sequence ID" value="BAD41406"/>
        <property type="gene ID" value="STH2421"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2421"/>
    <dbReference type="eggNOG" id="COG0173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_3_2_9"/>
    <dbReference type="OMA" id="YQLDVEM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
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        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000110963" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="593"/>
        </location>
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    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="222"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="542"/>
            <end position="545"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <sequence length="593" mass="66824" checksum="2DA0757A53D18CF4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT8</accession>
    <name>Q67LT8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2373"/>
    <dbReference type="EnsemblBacteria" id="BAD41358">
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        <property type="gene ID" value="STH2373"/>
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    <dbReference type="KEGG" id="sth:STH2373"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="9940" checksum="26DDA36B77E2E1AC" modified="2004-10-11" version="1">MPRREIGRDPGAWWRPRLRSRIPSRGPLPLRRGARSAPGSSGTSWTRAGPVRLPASGSMCGRRRSGKTSPGGGRPMVTSTMCSGRGPWAACGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU4</accession>
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        <submittedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41352">
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        <property type="gene ID" value="STH2367"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HAD-SF_hydro_IA"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="216" mass="23261" checksum="B688D717E764AA58" modified="2004-10-11" version="1">MEEETLSFKCVAFDVDGTLLDTEKAVLVSLQKVLLEETGQTPAEEELRFVLGIPGVTALEQLGVPDPGAAFDKWVAYAREVDHLVTVFPGIVELLDELQDAGVTLGLVTSRTRIELDGDLVHFGLLERFRAVVCADDTEGHKPDPDPLLKLMADTGFSPGEVLYIGDTVYDSQCARAAGVKFALALWGAGDPNVPCDYRVAHPAELAAICRPAPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV1</accession>
    <name>Q67LV1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2360"/>
    <dbReference type="EnsemblBacteria" id="BAD41345">
        <property type="protein sequence ID" value="BAD41345"/>
        <property type="gene ID" value="STH2360"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2360"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028469_1_0_9"/>
    <dbReference type="OMA" id="FAQIVWS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="559"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="619" mass="64648" checksum="D8D6F8AAB291F32B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX0</accession>
    <name>Q67LX0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sugar ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2341</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196464.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2341"/>
    <dbReference type="EnsemblBacteria" id="BAD41326">
        <property type="protein sequence ID" value="BAD41326"/>
        <property type="gene ID" value="STH2341"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2341"/>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_92_3_9"/>
    <dbReference type="OMA" id="IKMLSGA"/>
    <dbReference type="OrthoDB" id="551294at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="256"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="511" mass="56120" checksum="6625FFBFD761E653" modified="2004-10-11" version="1">MEPLVRMDHITKTFPGVRALSDITFDIRPGEVHVLLGENGAGKSTLMKILSGAYEPTSGTITVDGRAYPRLTPALSAKLGISIIYQELSVINELSIEENIFVGRLPVRKVMGVPVVDYAFMRQRTQELLDILDLRRSPETLVGDLSISEKQMVEIAKAVAFNARVIIMDEPTSSLTDAEVEKLFAIIRRLKGEGKGIVYISHKLKEILAIGDRITVLKDGTYVGTRDVQDVTMDGLVTMMVGRELKDKYQANTDVHRTGKVIFEVRNLTRRDGRVQDVSFALREGEVLGFAGLVGAGRSETMCAIYGTDPIRSGEIWLHGERMEIRSPYDALKKGIGLVTENRRETGFFPNFSIRRNVAIARQLKLARWGGLWGLVNERDEQKTAEKARADMQIKCASLEQNITELSGGNQQKVILGKWLAAGVKVLIFDEPTRGIDVGTKAEIYKLMRSLAESGIGVIVVSSELPELLSVCDRIIVMSEGRISAEFTAAEATEEKIVKAATVGAGASGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY6</accession>
    <name>Q67LY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196448.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2325"/>
    <dbReference type="EnsemblBacteria" id="BAD41310">
        <property type="protein sequence ID" value="BAD41310"/>
        <property type="gene ID" value="STH2325"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2325"/>
    <dbReference type="eggNOG" id="COG1234">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_3_1_9"/>
    <dbReference type="OMA" id="HIPPWTD"/>
    <dbReference type="OrthoDB" id="1712770at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="18"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="244" mass="25927" checksum="B56D24F9395EE3BD" modified="2004-10-11" version="1">MLVTVLGRYSPYAPVGGACPGLLVQARGVALMLDGGPGTVARLQMQVPAAHLTAVLVSHLHHDHIADLHCLQYLMADQAPGREPLPIYAPGGDNPDRRWLEPSSFGARWVRLLPLPVDEGLAVGPLRVTFARTDHPEPCWAMRITDGCRTLVYTADTGAGVDLAPFARGADLLIAESTFVAANGQNRRGHLTAAEAADLALRAGVGRLLLTHFLPSTPAAEAEAEARAIFPPAEAAVEMRTYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M06</accession>
    <name>Q67M06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2305"/>
    <dbReference type="EnsemblBacteria" id="BAD41290">
        <property type="protein sequence ID" value="BAD41290"/>
        <property type="gene ID" value="STH2305"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2305"/>
    <dbReference type="eggNOG" id="COG0563">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092618_1_1_9"/>
    <dbReference type="OMA" id="WVLDGNY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="21408" checksum="D90AA84C2885803F" modified="2004-10-11" version="1">MIGSSGSGKTTLARELAMRLGVPHIEADALYWGPNWTPAQPDEFHRRVDQATAGDRWAFDGNYSVVHRLVWSRADTVIWLDYPLGIILWRLLRRSVRRSVLGEELWNGNRESLWKHLLPRDSLFYWVLTTHRRRRQRYEATLQQPEYAHLRFIRLRSPRDTAKWLASVPTTATLREDAGASTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M26</accession>
    <name>Q67M26_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2284</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41269">
        <property type="protein sequence ID" value="BAD41269"/>
        <property type="gene ID" value="STH2284"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2284"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269531" evidence="2">
        <location>
            <begin position="26"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="840" mass="89268" checksum="5EA0A351631C81B0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M54</accession>
    <name>Q67M54_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2256"/>
    <dbReference type="EnsemblBacteria" id="BAD41241">
        <property type="protein sequence ID" value="BAD41241"/>
        <property type="gene ID" value="STH2256"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2256"/>
    <dbReference type="eggNOG" id="COG3858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_754250_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269268" evidence="2">
        <location>
            <begin position="23"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="3">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="3">
        <location>
            <begin position="44"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="382" mass="41716" checksum="945BB598646F4648" modified="2004-10-11" version="1">MRRVLALLMMLTMMLVPALAQAETAVRLVVDGVEVQTDVAPVLENDRTLVPIRAVTEALGFEVEWDQETRTATLTKGETTIQLTVGSPEAVVNGEKVALDVAPFIVSDRMMVPVRFVAEEIGLLVDWEQETRTVLITSQPPADEAAETGESDEDGEADEAGEPAGVVEPAALELLAQAHVASEVNVRQTGHFTVTIEGGLIPVDSEIFLEMYQEAPERALGYNTVRAFGMEQTIGVAVLDGQYWMQDETGAWAQMVMEEMAPTDRLSDPSALVNLNPAEYDWASATVTREAYEEAELQVVTVVMDKSGLAALIGEASELITDVRMEARYWLNDDGTLHHIDVYVETIADEPVPIRVVMQGTVFIEPWDGTIEFPPEITGAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M59</accession>
    <name>Q67M59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196375.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2251"/>
    <dbReference type="EnsemblBacteria" id="BAD41236">
        <property type="protein sequence ID" value="BAD41236"/>
        <property type="gene ID" value="STH2251"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2251"/>
    <dbReference type="HOGENOM" id="CLU_677799_0_0_9"/>
    <dbReference type="OrthoDB" id="1003112at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="45973" checksum="F898EC20E9B4DC32" modified="2004-10-11" version="1">MHTDPPDPRRGQVCSLEVARTDHLVRRLRSKLMDYLRDDEAEISVGWGMVRFFPPELFDEVEPEARSLAVDWLAFMFPGEWRVPLARQLAEDDASLSQEERATLNAWADRAAPGFFRVESVSGPQARLVRLPDDAPRTVRGVRGGVLKPGDLLVTWLLPTGPAYYLGLETAFVDQDLTDGLRHILQVEMDLLRRQRPRATWDDLYRTSWPRIVWAVGVLANDADVFRIHAPPGPSVLWDGRPVPDAPAWWERTAEWARTLAETSAPIHEEPGDGIERLWWDAALTLRPKQERTAASWTAAVVYAFRRYVQGDPTVTQAEVAEQCCVSPATVGNRSRQIVKALGLEPFDLRYVDLLARDVRILWEMHCIGALGSPALYGGMDVAKMSPEDKLLLHAVLRDMRRYLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB4</accession>
    <name>Q67MB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2194</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2194"/>
    <dbReference type="EnsemblBacteria" id="BAD41179">
        <property type="protein sequence ID" value="BAD41179"/>
        <property type="gene ID" value="STH2194"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2194"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="MDHGQMV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="26758" checksum="86AFF19B783F9FA2" modified="2004-10-11" version="1">MKPVSLLELEGVETYIGEYHILQGVSFAVRPGAITVLLGRNGAGKTTTLRTIMGLNPARRGRIRYKGKDITRLPPHEVARLGIGYVPEDRGLFGSLTVAENLRVAVRGGDLDRQVRRRGPGAATGRLAEVLELFPDLKRAWNRPAGTLSGGQQQMLAIGRVLLSDNDLLLIDEPSKGLAPVVVEHLGEALRKIAARTTVLLVEQNFGLAQAVAEHCVVIDDGRTVHQGSMADLLDDPVLRQRYLGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME6</accession>
    <name>Q67ME6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="4 9">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2162</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + 3-methyl-2-oxobutanoate + H(+) = (R)-N(6)-(S(8)-2-methylpropanoyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10489"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the BCKDHA family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="4 9"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2162"/>
    <dbReference type="EnsemblBacteria" id="BAD41147">
        <property type="protein sequence ID" value="BAD41147"/>
        <property type="gene ID" value="STH2162"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2162"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_5_0_9"/>
    <dbReference type="OMA" id="LQYWGGD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034616">
        <property type="entry name" value="BCKDH_E1-a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="PTHR43380:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="E1_dh" evidence="10">
        <location>
            <begin position="43"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="352" mass="39077" checksum="89E537A6E899F34D" modified="2004-10-11" version="1">MQAVDFQVSEHRDGRGRAMKEATMARHEALGLTADQVREMYYYMLLTRRLDERLWLLQRGGKIPFVISPQGQEAAQVGAAFAFRRRQDWFTPYYRDLGVNLVVGVTPREVMLSAFARGADPASGGKQMPSHWGNRPLNIVSGSSPVTTQVLHAVGIAQAARMRGDDVVVYTACGEGSSNQGDFHEALNWAGLHKLPVIFMVQNNEYAISVPLSQQVAGGSVAARGRGYGMPGVEVDGTDVLAVYEVVKEAHERARRGEGPTLIEARCIRITSHSSDDDQRRYRDPEEIAAVQVRDPIRKARQYLFEHGLMDEAAEQELERKVAAIVDDATDWAEAQPYAAPEEALRHVYKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH3</accession>
    <name>Q67MH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196260.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2135"/>
    <dbReference type="EnsemblBacteria" id="BAD41120">
        <property type="protein sequence ID" value="BAD41120"/>
        <property type="gene ID" value="STH2135"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2135"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_555393_0_0_9"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="370"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="491" mass="54614" checksum="FCD59F2CE8796996" modified="2004-10-11" version="1">MGHRQPNDVGAVTGLHWSGRVFMAALSAFAVASAFAGWLLTRRPPMWSLAWWGPGIGAALGFVLTFSAFWRVYVTVPWLPWAFLALDGLAVILFWSDPDSPFGPLLLLLLSAVVAARFRKWFLMAHVWILAVALAGAWFLGADASALLFLVSGLSGVIGVIRAIYLRFREQAIQTDHLRRLMELLPVLKAHGVQDVVEAAVRHIVRATNSRTGVVLLLDEEAQVLRPHFLYSEPPLSPEEKEAVMNTTIEVGVGLSGWVAKHGQSILTNDAARDPRAAHVPGTPHEDESILVVPLIADGRLYGVLRVDRPGVDQYTIGDLHLLELMAAHVSDAISRAQLEERLARRDALTGVYNRHYLNEWSQHAMREVREVSVLLIDLRKFKEVNDRWGHLTGDRVLRQCAELICGSVRTGDLVVRYGGDEFLVVLANTTRAEAERIAARIQERVAEWNAQQGEGEPRLILDIGVDTAQQSEFQQLLDRADERMYACKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH8</accession>
    <name>Q67MH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2130"/>
    <dbReference type="TCDB" id="9.B.151.1.3">
        <property type="family name" value="the uncharacterized 7 tms archaeal/bacterial protein (7-abp) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41115">
        <property type="protein sequence ID" value="BAD41115"/>
        <property type="gene ID" value="STH2130"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2130"/>
    <dbReference type="HOGENOM" id="CLU_1045560_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="27995" checksum="BE5D039AC4AA307E" modified="2004-10-11" version="1">MAALVSRAGASGRNTGRAGLTPPSRQANIQSIRIRNACGERGVLQMMTIANSVLVVLSLLLGGRLLMHYRNKPRPHSLWYGVGLILIACAALPELVYELTGQVPAVLWWLYWSSASSCVAFLSVGSAYLISQKFGKGALVTAAVTTAWVVAATLLTGGMGPAVLSSEAFRSAPTGAIKLPFLLQNICGAMLILVTAIMSFVRTRAPFALLIAGGVFMFASGGASAGLLEFSQIFAFTQTAGILLLYAGVSLSLRPRQRPQHQSVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ1</accession>
    <name>Q67MJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2117"/>
    <dbReference type="EnsemblBacteria" id="BAD41102">
        <property type="protein sequence ID" value="BAD41102"/>
        <property type="gene ID" value="STH2117"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2117"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_9"/>
    <dbReference type="OMA" id="HVRLLCN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="29497" checksum="6A932CD4E5090625" modified="2004-10-11" version="1">MVAQPSLSQQIRRLEEELGVLLFDRSRTPVRLTDAGEALLPHAEAILRQVEAAGAAVEERIGLRSGRLVLGSLPVTGSRLLPRAITAYRQRYPGVQITLREEPTQRLTELALADETDLTLTTLPVGSDELDWQPLLTEPILLALPPDHPLAADGPPHPAIPLSAVAGEPFLIMKPGYGFRDLVLAACQAAGFSPQIAFESAQIETLQAMVAAGLGVTLVPQMAADRSLRPSPVFVPLAGAPLTRTLALAWRRDRPLPRSAQAFLKLASELWPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ7</accession>
    <name>Q67MJ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2111</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196236.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2111"/>
    <dbReference type="EnsemblBacteria" id="BAD41096">
        <property type="protein sequence ID" value="BAD41096"/>
        <property type="gene ID" value="STH2111"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2111"/>
    <dbReference type="eggNOG" id="COG2510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120467_0_0_9"/>
    <dbReference type="OMA" id="VNSDFAT"/>
    <dbReference type="OrthoDB" id="1668457at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="138" mass="14815" checksum="ADA6086DCBF6CC72" modified="2004-10-11" version="1">MWKLYAVLSAVFAALTSILAKIGIKGVDANLATAIRTTVILFLSWGIVFAIGEHHAIRSLTRHNWTFLILSGLATGLSWLFYYKAIALGDVSKVAPIDKSSIVLTLILSYLILGEPFTPKTLLAGGLVVAGTFLLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML7</accession>
    <name>Q67ML7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196216.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2091"/>
    <dbReference type="EnsemblBacteria" id="BAD41076">
        <property type="protein sequence ID" value="BAD41076"/>
        <property type="gene ID" value="STH2091"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2091"/>
    <dbReference type="HOGENOM" id="CLU_2385054_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="94" mass="10052" checksum="F391EB5165A967A4" modified="2004-10-11" version="1">MRRNRTALVGAALGSALAMPLFEVCLLTILLVDGSLLDATVAVRYFSRPLNLIGLLASLHVLGALVGAAVGYMHGRMRPAPRLRVIPGGYRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW7</accession>
    <name>Q67MW7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage V sporulation protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoVD</name>
        <name evidence="2" type="ordered locus">STH1991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1991"/>
    <dbReference type="EnsemblBacteria" id="BAD40976">
        <property type="protein sequence ID" value="BAD40976"/>
        <property type="gene ID" value="STH1991"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1991"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_3_9"/>
    <dbReference type="OMA" id="VPYGWFV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transpeptidase" evidence="1">
        <location>
            <begin position="241"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="550" mass="58750" checksum="CE98CC99C4A5D3A7" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY9</accession>
    <name>Q67MY9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1969"/>
    <dbReference type="EnsemblBacteria" id="BAD40954">
        <property type="protein sequence ID" value="BAD40954"/>
        <property type="gene ID" value="STH1969"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1969"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061540_1_0_9"/>
    <dbReference type="OMA" id="PYTTFMA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMT1" evidence="1">
        <location>
            <begin position="60"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="353" mass="38583" checksum="8AEB6849576B651B" modified="2004-10-11" version="1">MGGVQGEDGQMWNRRFRPAGAALALVLTAALLAACSPRSAPKDEKFTVRFTEVIHSIFYAPQYVAQAKGFFADEGLEVVSSTAQGSDRGAAALLAGTADIAMVGPETAVFVHNQDSPVKVKIFAQLTARDGSFLLAREKPEGEFSWTDVKGKSIIGWRPGSMPQLVAEAILRQHGLDPETDLEYIDNLAATAMVGAFLSGQGDYIQVFEPSVSELVQGGHAYLATYLGADYGEMPYTAFLATDKFIAEHPDVIQRYTNAIYRAQKYIFEADPKVVAQEVAQFFEGTDVEILASSIQRYRDEGGWVHTPVMKPEDYEKLQDLMIAGGVLEPDQRAPFDAIATNRFAEEAVRQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N19</accession>
    <name>Q67N19_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PTS system IIB component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1939</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1939"/>
    <dbReference type="EnsemblBacteria" id="BAD40924">
        <property type="protein sequence ID" value="BAD40924"/>
        <property type="gene ID" value="STH1939"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1939"/>
    <dbReference type="eggNOG" id="COG3444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116175_1_0_9"/>
    <dbReference type="OMA" id="QVATMWS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.35.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004720">
        <property type="entry name" value="PTS_IIB_sorbose-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036667">
        <property type="entry name" value="PTS_IIB_sorbose-sp_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03830">
        <property type="entry name" value="PTSIIB_sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52728">
        <property type="entry name" value="SSF52728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51101">
        <property type="entry name" value="PTS_EIIB_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIB type-4" evidence="8">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="17569" checksum="493FCEAA84E33501" modified="2004-10-11" version="1">MVHMRIDNRLIHGQVTVAWASHLSADHMIVCNDKVAQDPIQKMMLPAAARGVKTSVLSVADTLAYCASPEAEQERIFIIAKFPSDALALLDGGLKPQEINVGNQAPIPGTKFTMVTRTCAVTPEDAAIYREIVKRGYELTGRMVPADPKNDFIELIAKKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N20</accession>
    <name>Q67N20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1938</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196065.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1938"/>
    <dbReference type="EnsemblBacteria" id="BAD40923">
        <property type="protein sequence ID" value="BAD40923"/>
        <property type="gene ID" value="STH1938"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1938"/>
    <dbReference type="eggNOG" id="COG3356">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030011_5_0_9"/>
    <dbReference type="OMA" id="IVFCTAH"/>
    <dbReference type="OrthoDB" id="704008at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="44994" checksum="7BC54924F68AF20C" modified="2004-10-11" version="1">MKAGHHCAIITPPIPCGMGGYAARSGPAEGVHDPLFARALVLEAGGERLGIITCDILHLERPVVEAARARAAELTGIPPERVMLLASHTHSGPSPGDWSTHPSPPEYRAWLPLQLAGCLLQATRQMAEVDLAWAEAPVEGLGKNRTDPDLPFDPTLRLLALLAGGRPRAVLLNYGCHPTVMGPENRLISADWPGAAVAALRRALGDDVWVGFAQGCAGDVSARFTRREQSFAEVERHGWLLAGAALTALGRLGGPEGGSLSRPLRLGARSRVVRLEARRLPSAEEAALQVESARARVAELEAAGASHAELRIAQTALQGAELALRYATEGIPVDLNCEVQAFAIGDAALVSLAGEPFSALGQAIRSRSPFAVTLVAGYGNGYCGYIPDRAAFARGGYEALSAPSEPGSGERLVDAAADLLSELKAEVAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N44</accession>
    <name>Q67N44_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1914"/>
    <dbReference type="EnsemblBacteria" id="BAD40899">
        <property type="protein sequence ID" value="BAD40899"/>
        <property type="gene ID" value="STH1914"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1914"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_778276_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="38860" checksum="1C6054B9935A4415" modified="2004-10-11" version="1">MDTLEGPGRFTLDLPDGGWYLHAVGGIPDDRGTLNGLGWGSHGGIYGFGRPVAPGEPVRIHVSPLWRDLTHLPLIRGAMLSDDQWHALHRAMARLHEDLSCNIEGDLERAASLTRTRLSALFRRGVGVTMEEYRTRVRLEAAKALLVLSDAPVRDVALEVGYSAPAQLRRMFLRYLGVTPGEFRRLAQAVSESGPAQPPAAGSARRYGLAGALSRWLGPPRTGTVRGEVLYRGDREGRALYIGLFPGPLPDTYPAVWTAIPAPGPFTLRRVPEGRYYVLAAYLAARMRYPGDLGSGFAYGGYGDVDTSGDLARWNPVPVTVRPGDEVAGLRIELVDGEIIRRFGGTWLRAFLPDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N88</accession>
    <name>Q67N88_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1870"/>
    <dbReference type="EnsemblBacteria" id="BAD40855">
        <property type="protein sequence ID" value="BAD40855"/>
        <property type="gene ID" value="STH1870"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1870"/>
    <dbReference type="eggNOG" id="COG0170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058561_7_0_9"/>
    <dbReference type="OMA" id="VASICWG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="27921" checksum="917C3636AEFE4F82" modified="2004-10-11" version="1">MGVQPPVKRVASVSTPEPDIPCGTNGGYDMLTDLVAGFGKLVVYYGLCVIGALALRRFVRVPAEVFRKTLHLILLGSLFVFLYGFGTWTASALAAVLFVAAVYPALAAAERLPGYSQLLTERKAGEIKRSLVVVFAMYATLAAVGWGWLGERYLVTASVMAWGLGDAAAALVGKRFGSHFIEGRLVEGRKSVEGTLAMFLVSFLSVLAVLLVHSAGNWRVSAPIAAVTAAVSAAVELCTRDGMDTITCPFAAAAVLIPLVRALGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB1</accession>
    <name>FOLD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">STH1847</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195975.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NB1"/>
    <dbReference type="SMR" id="Q67NB1"/>
    <dbReference type="STRING" id="292459.STH1847"/>
    <dbReference type="EnsemblBacteria" id="BAD40832">
        <property type="protein sequence ID" value="BAD40832"/>
        <property type="gene ID" value="STH1847"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1847"/>
    <dbReference type="eggNOG" id="COG0190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_2_1_9"/>
    <dbReference type="OMA" id="HIVGRPM"/>
    <dbReference type="OrthoDB" id="1449514at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000268528" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="286" mass="30374" checksum="E3A018F64C3DB3C7" modified="2004-10-11" version="1">MTAGVIDGKQVAAQVQEEVAREVAALKAEGLTPGLAVVLVGDDPASKVYTANKERTAKELGMHSVLYHLPATTTQQELADLVMQLNRDPAIHGILVQSPLPEGLDMDAVIRLIDPRKDVDGFHPENVGRLWIGQDGLVPCTPAGVMRLLDAYGVDPKGKHAVVVGRSNIVGKPMAALLLQRHATVTICHSRTPDLAETCRRADILVAAVGRLQMITAEHVKPGAVVIDVGINPVPGFKKRIRGDVDFDSVQEVAGLITPVPGGVGPMTIAMLMANTVKAARMQTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC5</accession>
    <name>Q67NC5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage IV sporulation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoIVB</name>
        <name evidence="2" type="ordered locus">STH1833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195961.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1833"/>
    <dbReference type="MEROPS" id="S55.001"/>
    <dbReference type="EnsemblBacteria" id="BAD40818">
        <property type="protein sequence ID" value="BAD40818"/>
        <property type="gene ID" value="STH1833"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1833"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035713_1_0_9"/>
    <dbReference type="OMA" id="TMTFYDP"/>
    <dbReference type="OrthoDB" id="1237700at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014219">
        <property type="entry name" value="Pept_S55_SpoIVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008763">
        <property type="entry name" value="Peptidase_S55"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05580">
        <property type="entry name" value="Peptidase_S55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02860">
        <property type="entry name" value="spore_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51494">
        <property type="entry name" value="SPOIVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S55" evidence="1">
        <location>
            <begin position="192"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="469" mass="50916" checksum="162C0C9A131D7DAD" modified="2004-10-11" version="1">MSTREKWRRAAGLLLVVLILSVCATPEFRTFVSLPDHLRLPQGQAHELSLGIPFSTAVPTSGQGLAVQRSGSPLQLAAEQTGRYTLDVKLFGLIPIRRMTVDVVPEIRVVPGGHSIGVRLQSDGVQVVGFAAVEDEEGRVHHPGRAAGLQVGDTILAIDGRPVRGEDHAVHLFQEAGRRGREITLTVRRGDERLERTVKPVREKETGRWRVGLYIRDGASGVGTLTFYHPPTRKFGALGHVIAEGESRRPFQFAHGQITAADVVKIQKGRRAAPGEKITSHPDSGGRLGVIEKNTPFGIFGRLNSPLKNPLYEEPLPVAMASQVKEGPAEMLTVVDGQKIERFQVEIVRLIRQPTAEGKNMIVQVTDPRLLEATGGIVQGMSGSPIIQDGRVVGAVTHVFVNDPTRGYGVLIEWMLQEAGLLPAERNTEPNQSGLREDTLLPEVLSGRAVRGGGRRAGNVCVTSMCVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE6</accession>
    <name>Q67NE6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase sigma factor similar to B. subtilis sporulation-specific sigma-F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">spoIIAC</name>
        <name evidence="6" type="ordered locus">STH1812</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195940.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1812"/>
    <dbReference type="EnsemblBacteria" id="BAD40797">
        <property type="protein sequence ID" value="BAD40797"/>
        <property type="gene ID" value="STH1812"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1812"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_5_9"/>
    <dbReference type="OMA" id="RDDGMIK"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014322">
        <property type="entry name" value="RNA_pol_sigma-B/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014236">
        <property type="entry name" value="RNA_pol_sigma-F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02980">
        <property type="entry name" value="SigBFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02885">
        <property type="entry name" value="spore_sigF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="5">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28836" checksum="269153BE8C2ED0C1" modified="2004-10-11" version="1">MAEENTLEERGWPDEEVRRLVALARAGDKEARDRLVEGNLRLVRSLVSRFSASGADPEDLFQIGCIGLLKAIDRFDLNYDVRFSTYAVPLIMGEIRRHLRDDGPMRVSRSLKQLAQQARRVRERLAAQMERDPTVAEVAAELGVSPEEVVAALDGVRPPTSIHQTVHEGDGDPIYLLDQLAADDGQREGVWLDRVALREGLNQLDERERTVILLRFFRDKTQTEVASILGCSQVQVSRLERRALERIRNYVNAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG8</accession>
    <name>Q67NG8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spore peptidoglycan hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1790"/>
    <dbReference type="EnsemblBacteria" id="BAD40775">
        <property type="protein sequence ID" value="BAD40775"/>
        <property type="gene ID" value="STH1790"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1790"/>
    <dbReference type="eggNOG" id="COG3858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_611013_0_0_9"/>
    <dbReference type="OMA" id="HTVYFED"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02874">
        <property type="entry name" value="GH18_CFLE_spore_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041704">
        <property type="entry name" value="CFLE_GH18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="3">
        <location>
            <begin position="133"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="448" mass="47764" checksum="E54D51EA1282DDE8" modified="2004-10-11" version="1">MASATSHTVSACLFQHPSAPHGRVKRPPRHHPANNSQLAGNRHHRYNLTRHIFVLDYIGGGAVRRLGRPLWVGLLIGALLLAGTVVAGAPGLPAGALPVPAELQSGRSWVLPDLAGDGWVVLTAEPPAVDEGPVVVAYYAEDWEGDVRALNALRRAGARVDRVVNFALELHADGSVTTRSYPALAAAAAELGVPVYGLVHNMQNGQFSAEIARAVLSDPARRSRAVADMLAIAREHGLAGIDIDIENVPGDLRAGYTALAAEAAAVLKPGGIRLTLSVPAKTWDDTTSNWGGAFDYAALGEIADEIAIMAYDEHTWGLPHGPVASLPWVERVVAYAVTQIPPEKILLGIPAYGYDWIAGTRQVVGGLSTGGAYSLAARHGAEVRWDDEAQVPWFRYVADGAEHIVYFENSRSAAAKLRLVGRYGLGGIAIWRLGLNEDGLWEVVVDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NK2</accession>
    <name>Q67NK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195884.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1756"/>
    <dbReference type="EnsemblBacteria" id="BAD40741">
        <property type="protein sequence ID" value="BAD40741"/>
        <property type="gene ID" value="STH1756"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1756"/>
    <dbReference type="eggNOG" id="COG0860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_754250_0_0_9"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269462" evidence="1">
        <location>
            <begin position="25"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="30"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="47"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="38579" checksum="A76FD888913C2CB0" modified="2004-10-11" version="1">MRRFFVVLLMLILIPTAVPGTALAEEPVRLFVNGSQVVPDVAPVIVSDRTLVPVRFLAEPLGFAVEWDGATGTVTLSGARVIRLAVGRPEAIVDGTVVALDVAPVNVDGRVMVPLRFVAEQMGAAVEWDPQNRVVTVTAARILPEGQVGGDLALVGNVEALGAWAEGHTTGQFRLSAVAGVLSMDYTFALDGYREGDDLLVYLTGAGPGLDYRSALAVRAGKVWRLGPGGTWVRRPEEDAAFGPDGLPGWGGDLPAEGGLFDPDTTELAGARILREERTLEGVTYDVVVVEWDQAALSPLFAEFPPTVGDASLLLTVYALPDTGGPVRVDLSMHAADATGVTVDLSGSLAVKPLEGAIPFPPEILSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-21" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP7</accession>
    <name>COMB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable 2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">comB</name>
        <name type="ordered locus">STH1711</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NP7"/>
    <dbReference type="SMR" id="Q67NP7"/>
    <dbReference type="STRING" id="292459.STH1711"/>
    <dbReference type="PRIDE" id="Q67NP7"/>
    <dbReference type="EnsemblBacteria" id="BAD40696">
        <property type="protein sequence ID" value="BAD40696"/>
        <property type="gene ID" value="STH1711"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1711"/>
    <dbReference type="eggNOG" id="COG2045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070028_0_0_9"/>
    <dbReference type="OMA" id="RLFMSTT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00490">
        <property type="entry name" value="ComB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000081473" description="Probable 2-phosphosulfolactate phosphatase">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00490"/>
        </source>
    </evidence>
    <sequence length="233" mass="25210" checksum="211315F45620BCDF" modified="2004-10-11" version="1">MVNIPPAEELAGRVAVVIDVLRATTTICTALANGADTVVPILSPEEAFQVARDNPDRQFLLGGERKAVLIPGFHCDNSPLEYTEARVKGRPILFTTTNGTRAIRRAAGADRVYIASLLNAPAVGRELARLEVDVAICCAGTHDQFSLEDTVCAGAILEYMAGPDRPVETNDMGLVARELFRRYDGRLADLLHLSSHGQNLVRLGMQDDLLFCAQLGTLTILPVFTEGQVVLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU9</accession>
    <name>Q67NU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1659</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1659"/>
    <dbReference type="EnsemblBacteria" id="BAD40644">
        <property type="protein sequence ID" value="BAD40644"/>
        <property type="gene ID" value="STH1659"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1659"/>
    <dbReference type="eggNOG" id="COG1863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086615_3_2_9"/>
    <dbReference type="OMA" id="HAMDIED"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="PTHR34584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019239">
        <property type="entry name" value="MrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="17944" checksum="BC0CCA190191F89C" modified="2004-10-11" version="1">MLFAFLWMILQESYTLGSFVGGLVVGLVILLIFPAPVHGLINPYVRRPGGIFRWLVSAAHLFLYYTRHWLRGNAEMARLMLRRDLSTITPGVLKFPLRVRQPGQVALLANLITVTPGSYTVEVSDDWDVLYLHVIDASDAEAALAPIRRIEELIMEVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P41</accession>
    <name>Q67P41_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gamma-D-glutamyl-L-diamino acid endopeptidase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1567"/>
    <dbReference type="EnsemblBacteria" id="BAD40552">
        <property type="protein sequence ID" value="BAD40552"/>
        <property type="gene ID" value="STH1567"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1567"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050685_0_0_9"/>
    <dbReference type="OMA" id="FHANEWI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06229">
        <property type="entry name" value="M14_Endopeptidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034274">
        <property type="entry name" value="ENP1_M14_CPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="54"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="400" mass="45029" checksum="80E28EF93981C265" modified="2004-10-11" version="1">MAGQYVVQQGDTFYRIAQRYRISVEALAAANPQVEDPNVIRPGMVLYIPARPPLQYIVQPGDTLWQIAQAYGTTVAALLQANPGVNPYQLRIGQPLTIPAASDGETIVVPREDYGYDEMMADLARLQQRYPFIEVRSIGRSVLGRDIPVVRLGTGPKEVHYNAAIHGEEWITAVLMMKFIEVYARARERGERIGNFDVPALWEQTSLFVVPMVNPDGVEISQEGVSRDNPYYDLLLQANGGSTNFRTWAANARGVDLNNQFPADWEREAARGPRSPAPRNYSGTGPLTEPEAIALAEFTRAHDFRLVIAWHSQGEEIYWGYEGLEPPESEQITKLFVDISGYRPVRYLQSWAGYKDWFIQDWRRPGFTLEVGRGVNPLPMSQFWPIWSRTIGVMLAGLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P50</accession>
    <name>Q67P50_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent clp protease proteolytic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195687.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1558"/>
    <dbReference type="MEROPS" id="S14.012"/>
    <dbReference type="EnsemblBacteria" id="BAD40543">
        <property type="protein sequence ID" value="BAD40543"/>
        <property type="gene ID" value="STH1558"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1558"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066827_1_0_9"/>
    <dbReference type="OMA" id="KMQDRVI"/>
    <dbReference type="OrthoDB" id="1399458at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="27495" checksum="8E4760DE5FCA32B5" modified="2004-10-11" version="1">MQDAPPQPIPGVSPGPDPAIPAQTPAPSPVVARSPKNAPLENLEAIGTTSVPQVSPNIHLLTIIGQVEGHLALPPQNKTTKYEHIIPQLVAVEQSPEIEGLLLVLNTVGGDVEAGLAIAEMVASLSKPSVSLVLGGGHSIGVPIAVSTTYSFIAATATMTIHPIRLTGLVIGVPQTYEYLDKMQDRVVRFVVSNSRISEERFRELMFRTGELARDVGTVVVGPDAVREGLIDEVGGLSDAVAKLNQLIAERKRTRPGVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P87</accession>
    <name>Q67P87_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L7AE family 50S ribosomal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1521"/>
    <dbReference type="EnsemblBacteria" id="BAD40506">
        <property type="protein sequence ID" value="BAD40506"/>
        <property type="gene ID" value="STH1521"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1521"/>
    <dbReference type="eggNOG" id="COG1358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157804_1_1_9"/>
    <dbReference type="OMA" id="MNKFYSM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L7Ae" evidence="1">
        <location>
            <begin position="5"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="103" mass="10660" checksum="7160A8F53FD7F69C" modified="2004-10-11" version="1">MLGLCQRAGKLVSGDLAAEQALRRRRGHLVLIAADASERTQSKFAHLAAQAGAPCYVVGTRADLGGAIGKAGRAVVVVQSRDFARGILGILRREGVAPVTGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA5</accession>
    <name>Q67PA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40488">
        <property type="protein sequence ID" value="BAD40488"/>
        <property type="gene ID" value="STH1503"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1503"/>
    <dbReference type="HOGENOM" id="CLU_1834161_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="15206" checksum="ADD7063F0A9020FD" modified="2004-10-11" version="1">MVAPVAAEPGVAVTVRYLTDVTWDRLTHTVVRDGEVVRRGAIRGREASFRLPDAPGEYTVTFRYTWGGPLAGTWGEGLWQFVVRLQERMQRRPGCGIAAARSLCPLLSTPGRTRRCAGPPCRSRGRPASEPAGGRGCSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD1</accession>
    <name>Q67PD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195607.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1477"/>
    <dbReference type="EnsemblBacteria" id="BAD40462">
        <property type="protein sequence ID" value="BAD40462"/>
        <property type="gene ID" value="STH1477"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1477"/>
    <dbReference type="HOGENOM" id="CLU_2557040_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="8853" checksum="F245734E332BC28A" modified="2004-10-11" version="1">MAEQDRRTVHEGPMADRRGPGWRQDAAELEARDRERKENPMGALPNNLTTIDKAHAARAKSDEGSVDPQADLGGGAAGESEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD4</accession>
    <name>RNH21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII 1</fullName>
            <shortName evidence="1">RNase HII 1</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB1</name>
        <name type="ordered locus">STH1474</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195604.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PD4"/>
    <dbReference type="SMR" id="Q67PD4"/>
    <dbReference type="STRING" id="292459.STH1474"/>
    <dbReference type="EnsemblBacteria" id="BAD40459">
        <property type="protein sequence ID" value="BAD40459"/>
        <property type="gene ID" value="STH1474"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1474"/>
    <dbReference type="eggNOG" id="COG0164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_2_1_9"/>
    <dbReference type="OMA" id="IHRYSFS"/>
    <dbReference type="OrthoDB" id="1685277at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07182">
        <property type="entry name" value="RNase_HII_bacteria_HII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_B">
        <property type="entry name" value="RNase_HII_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022898">
        <property type="entry name" value="RNase_HII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF18">
        <property type="entry name" value="PTHR10954:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000235776" description="Ribonuclease HII 1">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="68"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <sequence length="259" mass="28540" checksum="4A77FF94F58CA77A" modified="2004-10-11" version="1">MKRLQALMAEQGPDIYPEVIAALKDDPREGAQRLVRQCQTYLKERERQRAVLQRMYNYERQLWSMGYRHIAGIDEVGRGPLAGPVVAAAVILPGEVELPGIEEAKRLSDRRRMELYRRIREVAVAVGVGLVQPEGIDEASVVVATYKAMMKAVADLPVTPDYLLIDGVHLPNVSQPQVPVVGGETLSCSIAAAAVVAKVVRDEYMVEMDAKYPAYGFAHHKGYGTPEHRLALERYGPCPIHRKPSDGTPAGAVLLFTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF7</accession>
    <name>Q67PF7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="6">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195581.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1451"/>
    <dbReference type="EnsemblBacteria" id="BAD40436">
        <property type="protein sequence ID" value="BAD40436"/>
        <property type="gene ID" value="STH1451"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1451"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_9"/>
    <dbReference type="OMA" id="KMPERDY"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 6" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0753">Steroid metabolism</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="155"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="248" mass="25657" checksum="4F9220C3FFFF3832" modified="2004-10-11" version="1">MNLSTQVAIVTGATGGLGRAISLALAREGAAVVVNYARSAEKAAGLVEEISAQGGRALAVQADVGTAEGAEALVKAALDHFGRVDILVNNAGINRDTLIMRMKESDWDDVLDTNLKGAFLCIKAVSRPMMKARYGRIINIGSVAGLAGNAGQANYSAAKAGLVGLTRAVARELGSRNITVNCIAPGAIDAGMLLELSEEQRSNYLRQIPLERFGRPEDVAAAVVFLAGPGGAYITGQTIAVDGGLTMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF9</accession>
    <name>Q67PF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195579.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1449"/>
    <dbReference type="EnsemblBacteria" id="BAD40434">
        <property type="protein sequence ID" value="BAD40434"/>
        <property type="gene ID" value="STH1449"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1449"/>
    <dbReference type="eggNOG" id="COG1307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048251_0_1_9"/>
    <dbReference type="OMA" id="LHVQNKD"/>
    <dbReference type="OrthoDB" id="1288126at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="282" mass="30810" checksum="A0531D0200008777" modified="2004-10-11" version="1">MGIRIVTDSTADLPKELCRELGIEVIPLTVRFGEEAYLDGVTLDSDGFWAKLKESPHHPSTAQPAPGDFLEVYRRIHEAGDEIVSIHISSKMSGTVNSAEIAAQMLPEARISIVDTRSVSLGLGLVVIGAARMAREGKSREEIVAWAHKTCDRMNILFTIDTLEFLQRNGRIGKATALLGGLLGIKLILQVDKEGVVAPADKVRGRSRVFARAREIMHERVPPGRRIRMAVVHAQAPEQAQAWGEEVKRDYQVEEYLVGQLGAVVACHAGPGALGVIFHEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PI0</accession>
    <name>Q67PI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195558.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40413">
        <property type="protein sequence ID" value="BAD40413"/>
        <property type="gene ID" value="STH1428"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1428"/>
    <dbReference type="HOGENOM" id="CLU_2829689_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="66" mass="7344" checksum="44BDD555B607E574" modified="2004-10-11" version="1">MASSRIRRQLRQLFRLVRRLDGAGVSENLILAVVFFALSRDDRGTLAAFWQDDGSGEDDGSGDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ4</accession>
    <name>Q67PJ4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="11">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpB</name>
        <name evidence="13" type="ordered locus">STH1414</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1414"/>
    <dbReference type="EnsemblBacteria" id="BAD40399">
        <property type="protein sequence ID" value="BAD40399"/>
        <property type="gene ID" value="STH1414"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1414"/>
    <dbReference type="eggNOG" id="COG0133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_9"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="32"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="376" mass="40546" checksum="BA70F5A023094564" modified="2004-10-11" version="1">MAACIELEEAYRRYKDDPEFLEELTYYRRQYVGRPTPLYFAERLTRHAGGARIYLKREDLNHTGAHKINNALGQALLARRMGKKRIIAETGAGQHGVAAATVAALFGMECDVHMGEVDVARQRLNVFRMQLLGARVIPTRSGSRTLKDATNEAIRDWVTNVETTHYVIGSAVGPHPYPMIVRDFQAVIGYETMSQIQEAEGRLPDAVVACVGGGSNAIGIFHPFVPFPEVRLVGTEAGGLGIGSGKHGATLSGGGEVGVFHGARTYLLQDAYGQVQEAHSISAGLDYPGVGPEHAYLRDTGRAEYVAVTDEQALEGMQLLSRTEGIIPALESSHAVYYGVRLAAGMAPDQIVVINLSGRGDKDMAEVARILGGEGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR9</accession>
    <name>KGUA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gmk</name>
        <name type="ordered locus">STH1339</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195470.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PR9"/>
    <dbReference type="SMR" id="Q67PR9"/>
    <dbReference type="STRING" id="292459.STH1339"/>
    <dbReference type="EnsemblBacteria" id="BAD40324">
        <property type="protein sequence ID" value="BAD40324"/>
        <property type="gene ID" value="STH1339"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1339"/>
    <dbReference type="eggNOG" id="COG0194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_1_2_9"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="1502854at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000170624" description="Guanylate kinase">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <sequence length="211" mass="23261" checksum="DE67573342EF9D68" modified="2004-10-11" version="1">MPNKPKGLLIVVTGPSAVGKGTICRALLAETPGIRFSVSCTTRPKRPGEVDGVEYYFISKEEFERRIAAGEFLEWAEVYGNYYGTPRGYVEEVTAQGQDVILDIDRVGARAVREQYPDAVSVFVIPPSMEALRQRIAARGTESPEAVARRLAEAPEWIREGLTYDYVIVNDDLARAVAELRAIIMAEKARTVRNGAALIETLLEKGALTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW1</accession>
    <name>Q67PW1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1297"/>
    <dbReference type="EnsemblBacteria" id="BAD40282">
        <property type="protein sequence ID" value="BAD40282"/>
        <property type="gene ID" value="STH1297"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1297"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092639_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20344" checksum="A17F74ED6F9E0EA8" modified="2004-10-11" version="1">MQVDFAGDTVPIHDRETGKVWQASIFIAVLPASNYTFAKAYPAQDLRSWIKAHCEALEFFGGVPEIIVPDNPKAGVTRACRYEPDLNPAYQEMAAHYGAAVIPARPRSPRDKAKVENAVLVVERWILAALRNHTFFSLHDANRAIREAVARLNDRPFQKSRRNAPEPVRNAGQAGVAPFTAAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY3</accession>
    <name>Q67PY3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NifA family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1275</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1275"/>
    <dbReference type="EnsemblBacteria" id="BAD40260">
        <property type="protein sequence ID" value="BAD40260"/>
        <property type="gene ID" value="STH1275"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1275"/>
    <dbReference type="eggNOG" id="COG1221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3933">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014204_1_1_9"/>
    <dbReference type="OMA" id="ISMENFF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="2">
        <location>
            <begin position="107"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-4" evidence="3">
        <location>
            <begin position="559"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="PRD" evidence="4">
        <location>
            <begin position="817"/>
            <end position="916"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="916" mass="97981" checksum="473F9B6F5314FE23" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q42</accession>
    <name>Q67Q42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0749 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195347.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1216"/>
    <dbReference type="EnsemblBacteria" id="BAD40201">
        <property type="protein sequence ID" value="BAD40201"/>
        <property type="gene ID" value="STH1216"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1216"/>
    <dbReference type="eggNOG" id="COG3879">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040273_4_0_9"/>
    <dbReference type="OMA" id="IKVMDQR"/>
    <dbReference type="OrthoDB" id="1883872at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010273">
        <property type="entry name" value="DUF881"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37313">
        <property type="entry name" value="PTHR37313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05949">
        <property type="entry name" value="DUF881"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="44"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="26134" checksum="ACF5E53C686B8CCC" modified="2004-10-11" version="1">MSAQMRRVPWATALVGVALGLMLSMQFKVQRQVALADLAAVQRTEELVAQLAAAEALRDELAAEVEALRQQQLISVKTQNEYQALAERLEQAQIHAGLLPLTGPGVTVVMADSVLPVTPGENANNFIIHDEDVLRVINELRAAGAEAIAINGQRVTSRTEIRCMGPVVTINGVRTAPPLNIVAIGNPDELERAIMMKGGVAESLKYWGIQITVKKETSVRVPAYTASLRLNYAQPALEEVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QH7</accession>
    <name>Q67QH7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Endo-1,4-beta-glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1081</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195213.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1081"/>
    <dbReference type="EnsemblBacteria" id="BAD40066">
        <property type="protein sequence ID" value="BAD40066"/>
        <property type="gene ID" value="STH1081"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1081"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_9"/>
    <dbReference type="OMA" id="GLMITHI"/>
    <dbReference type="OrthoDB" id="900854at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="34932" checksum="6C9457D3D1D5C4AD" modified="2004-10-11" version="1">MSEKLLERVRELARLWGPSGREQRVHDALKAQIAPFVDEVRTDGFGNLLAVRKGREGGRRVLLSAHMDTPGAIALNVTDKGLIYLAPVGGLKVHHAVGQRVVWGSGALGVLQYEHVEEPKDLSFRKLFCDIGAATKEEALERVSLGDMCTLAGTVEAWGDLVVGPGLDNRLGCAVLLEVAERLGSGPHTVIFAFTAQGEVAPRGAAVAAFGAEPDVAVLVDSAVSGDQPGVRQVATRLGGGPALRLKDSGFMAHHGLAAQIAAAAEAGGIPLQRCVSPTGSEGWTVTPVGPGVPTAVIDIPVRYPGTGGEMADLRDARAAAELLLHFLGLPLNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI4</accession>
    <name>Q67QI4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1074</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195206.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1074"/>
    <dbReference type="EnsemblBacteria" id="BAD40059">
        <property type="protein sequence ID" value="BAD40059"/>
        <property type="gene ID" value="STH1074"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1074"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1069302_0_0_9"/>
    <dbReference type="OMA" id="VFITHID"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="28351" checksum="B0C8C5CEA8E980CF" modified="2004-10-11" version="1">MRQAGRVLVANPSLLERQRIASVLEAVGYEVVQAASLAEATQLLTEAAPRSVRAVLTETEFADGTVDDLIRQIRRDPAWVFTPVVVLGPAPPIPRLIELISLGASTVVNKPFSSQVLLRRLGESLAEAREREARGESPVRNLPDYLRREFRRADLGGFQVALMVVRLPDSVPEHRVPGFVETAARSLREYDLALRIGTRLVALVLAETGASGGEAVRRRLEALYPTPFSSGVAVYPDDATDPEELIRLARARAESALSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ1</accession>
    <name>Q67QJ1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD</fullName>
            <ecNumber evidence="1">6.3.5.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatD</name>
        <name evidence="3" type="ordered locus">STH1067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with MurT.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobB/CobQ family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.13" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1067"/>
    <dbReference type="EnsemblBacteria" id="BAD40052">
        <property type="protein sequence ID" value="BAD40052"/>
        <property type="gene ID" value="STH1067"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1067"/>
    <dbReference type="eggNOG" id="COG3442">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064047_0_0_9"/>
    <dbReference type="OMA" id="GTYMHGP"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140282">
        <property type="term" value="F:carbon-nitrogen ligase activity on lipid II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02213">
        <property type="entry name" value="Lipid_II_synth_GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043702">
        <property type="entry name" value="Lipid_II_synth_GatD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase_3" evidence="2">
        <location>
            <begin position="8"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="247" mass="27502" checksum="CD47FDFC9C3DC653" modified="2004-10-11" version="1">MERPRIHIGWLYPEYMNLYGDRGNIIVLTQRSRWHGLDPVVTPVRMGESADFARFDLLLIGGGQDREQSLIAEDFVQVKVPSLADAIEDGLAVLAVCGGYQMLGKYFKTHTGELIRFSGILDLWTEAGRERMIGNCVIESDLFGGTRTIVGFENHSGRTFLGKGLRPLGRVVKGYGNNGSDRTEGVVYKNTVGTYLHGSILPKNPHLADWLIQRALDRRYGGQVKIGALNDDLEMRAHEAIIARFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ4</accession>
    <name>Q67QJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1064</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195196.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1064"/>
    <dbReference type="EnsemblBacteria" id="BAD40049">
        <property type="protein sequence ID" value="BAD40049"/>
        <property type="gene ID" value="STH1064"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1064"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_0_9"/>
    <dbReference type="OMA" id="PLYRWSR"/>
    <dbReference type="OrthoDB" id="1452500at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="17"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="411" mass="42972" checksum="621F8A80846E48AB" modified="2004-10-11" version="1">MQQGLLAPQKRLGTAGLIALITVLNMTAPLSTDMYLPSFPTMMDEFGVTAGVLNLTLVGFFFFFAVGMLVFGPFSDRYGRKPVLVAGLGLYLAASLACAGAGSIWQLILFRVLQALGAGCMVSVSTALVKDCFDGRMRGTILATIQSMSVVAPMLAPIIGAFIVQYATWRTTFLMLAGISLCSLTAALLLQEPLPPGARLRGPILGSLGRLFVVARNPSFTSLLLVVALIPAGFMAYISVSSYIYQDFFGLSETAYSLFFATNAGVSVLGPLTYIAINRKVPPRLIITGALGLALCGGLAVLLLGRTAPLAFLLSFIPYSFGSSLIRPVSTNTLLSQQDSDTGSASALINFGNTAMGSLGMVLAALPWPDFVTGLGTIAAGCALLALLGWAVLLLSSIPLRGIKEGLSSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ5</accession>
    <name>Q67QJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD(P)H nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195195.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1063"/>
    <dbReference type="EnsemblBacteria" id="BAD40048">
        <property type="protein sequence ID" value="BAD40048"/>
        <property type="gene ID" value="STH1063"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1063"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_7_2_9"/>
    <dbReference type="OMA" id="PMNGWDE"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="23577" checksum="489D2B7C836D2C78" modified="2004-10-11" version="1">MTELPQNPCDVLEAIARRHSVREFRPDPVSEQVLLQLLEAARQAPSAMNLQPWEFVVVRRPETKARLAAATAGRNAQVVQTAGASVVCLASLRQADALADRIEGQIRAGQAPPDREAMVRRLREDQAYRQLLVLPNTYTAVGFLLLAATRLGLATLWMTGFDGEKVKEAVGAPAQDSLFAGVVAVGYPAEGWQQPARTRRPLEEIYSFERFGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM0</accession>
    <name>Q67QM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1038"/>
    <dbReference type="EnsemblBacteria" id="BAD40023">
        <property type="protein sequence ID" value="BAD40023"/>
        <property type="gene ID" value="STH1038"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1038"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_6_9"/>
    <dbReference type="OMA" id="CHATDPN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012854">
        <property type="entry name" value="Cu_amine_oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036582">
        <property type="entry name" value="Mao_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07833">
        <property type="entry name" value="Cu_amine_oxidN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55383">
        <property type="entry name" value="SSF55383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cu_amine_oxidN1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="132"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="617" mass="69895" checksum="8BE79430A0DA0FED" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP2</accession>
    <name>Q67QP2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1016"/>
    <dbReference type="EnsemblBacteria" id="BAD40001">
        <property type="protein sequence ID" value="BAD40001"/>
        <property type="gene ID" value="STH1016"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1016"/>
    <dbReference type="HOGENOM" id="CLU_1712343_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="16795" checksum="4A328AE1727C9A66" modified="2004-10-11" version="1">MSARLPRQHVYRSGHGCVRSTEARHPSQWDLDPEGWPALERLAGLPHWASACRIVSSTELKAVRTAECLARRNRLPPPEAVSALGERHKGSVVPNHAGVVVRLFRFPSQPAAQGWETVRAALAASTTRSRRWWRPPGGGTSSWSPTASCCRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ1</accession>
    <name>Q67QQ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195139.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1007"/>
    <dbReference type="EnsemblBacteria" id="BAD39992">
        <property type="protein sequence ID" value="BAD39992"/>
        <property type="gene ID" value="STH1007"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1007"/>
    <dbReference type="HOGENOM" id="CLU_2304622_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="100" mass="10389" checksum="0CF2C46FCCF5C7C1" modified="2004-10-11" version="1">MPIACRFARRGMLVAPRRLTAPGGPVSRVEGSTAGSGGDRSGGRDPIVELLAELRGRAVTLQVGQRLLTGRLILADPVVIVDGQGRATCARPEAVVAVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS7</accession>
    <name>Q67QS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH981"/>
    <dbReference type="EnsemblBacteria" id="BAD39966">
        <property type="protein sequence ID" value="BAD39966"/>
        <property type="gene ID" value="STH981"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH981"/>
    <dbReference type="eggNOG" id="ENOG5034AG5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140339_3_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012861">
        <property type="entry name" value="DUF1634"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07843">
        <property type="entry name" value="DUF1634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="108" mass="11271" checksum="6D01F485496BB7E9" modified="2004-10-11" version="1">MDSLQTRQHEVSRDVERVELAVAHLLRIGTAVAAALISAGLAVTLLQLSVSIGPTLVTAGIIALICTPLLRVAAAMVIYLRLGDVVYGVICLAVLLFVLAGMLLGEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ7</accession>
    <name>Q67QZ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transglut_core2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH911</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0162 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195043.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH911"/>
    <dbReference type="EnsemblBacteria" id="BAD39896">
        <property type="protein sequence ID" value="BAD39896"/>
        <property type="gene ID" value="STH911"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH911"/>
    <dbReference type="eggNOG" id="COG2912">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063810_3_0_9"/>
    <dbReference type="OMA" id="ANTKNFH"/>
    <dbReference type="OrthoDB" id="1023028at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglut_core2" evidence="2">
        <location>
            <begin position="104"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="30787" checksum="26216583ABD58801" modified="2004-10-11" version="1">MRTEGELRALVSLLADEQEGVARAAWDELLGAGQAAVPFLEAAFDDPDPRLRGRVRALLEELRLEALELQWRRLAQKDDDALDLEEGCILLAALGGTVGRERAVASFLDAVAGSVRAHMPAVGGLRAMGEVLFENLRFRAGEMGNPEHHFLPSVLERRRGIPIALAAVYILVGRRVGLPVYGVAMPDHFLAMYAEADRPAYVDCFNQGQVYRYETLSRILSRRGVVAPERVLSPCSTRVILYRMLGNLERLYTGVGQSRMVGRVQRWREILVVKGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R31</accession>
    <name>Q67R31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M23/M37 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH877"/>
    <dbReference type="EnsemblBacteria" id="BAD39862">
        <property type="protein sequence ID" value="BAD39862"/>
        <property type="gene ID" value="STH877"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH877"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_5_1_9"/>
    <dbReference type="OMA" id="GVRRYYN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M23" evidence="1">
        <location>
            <begin position="163"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="30164" checksum="221CDBA2E66AC1A6" modified="2004-10-11" version="1">MQLPASAPVITLRPAQVLQGDFAVLKVDQPLPGEPALHVEGLSEQPKLFLLDRHPTAFIGIPAAAPVGTYPVRLTWEGGEWEGTLEVVRKDFTVDRLEVTAEQEQIYYDPRQAEQWEKLYRLRSTSFPEPLWQGAFRPPLDGELWVTTYFGEIRIVNGVETGRHSGMDFGAPLGTPIYAPARGRVILAEEFIVSGRTIVIDHGLNLFTAYYHCEELLVQPGDWVEPGDPIGTVGSTGFSTGPHLHWTATVGNTPVDPWPLTQGSVADLFAGGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R90</accession>
    <name>Q67R90_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclic dehypoxanthine futalosine synthase</fullName>
            <shortName evidence="6">Cyclic DHFL synthase</shortName>
            <ecNumber evidence="6">1.21.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dehypoxanthine futalosine cyclase</fullName>
            <shortName evidence="6">DHFL cyclase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Menaquinone biosynthetic enzyme MqnC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mqnC</name>
        <name evidence="8" type="ordered locus">STH818</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Radical SAM enzyme that catalyzes the cyclization of dehypoxanthine futalosine (DHFL) into cyclic dehypoxanthine futalosine (CDHFL), a step in the biosynthesis of menaquinone (MK, vitamin K2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dehypoxanthine futalosine + S-adenosyl-L-methionine = 5'-deoxyadenosine + cyclic dehypoxanthinylfutalosinate + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:33083"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58864"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64270"/>
            <dbReference type="EC" id="1.21.98.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. MqnC family.</text>
    </comment>
    <dbReference type="EC" id="1.21.98.1" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH818"/>
    <dbReference type="EnsemblBacteria" id="BAD39803">
        <property type="protein sequence ID" value="BAD39803"/>
        <property type="gene ID" value="STH818"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH818"/>
    <dbReference type="eggNOG" id="COG1060">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_1_0_9"/>
    <dbReference type="OMA" id="ATMMFGS"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046992">
        <property type="term" value="F:oxidoreductase activity, acting on X-H and Y-H to form an X-Y bond"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00992">
        <property type="entry name" value="MqnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022431">
        <property type="entry name" value="Cyclic_DHFL_synthase_mqnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03699">
        <property type="entry name" value="menaquin_MqnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="76"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="393" mass="44174" checksum="4E522E97DDEEA408" modified="2004-10-11" version="1">MKPADRAEPEAQLERIHEALLRPEPTPAVERILHKALDGHRISYEEGCVLMREGDLGSLGLVAGEITRRFHPDNIVTFVVDRNINYTSVCVTDCKFCAFYRHEKDPDAWVLSHEEILQKVGELAATGGTQVLIQGGHHPKLPFAYYTEMCRLIKRHYPQVAIHSFSVSEIRHFARAYKMPVREVIERLKEAGLDSVPGAGGEVLADRPRRIIAPLKDPARDWLETYELIADCGLRGSSTMMFGHVETLEERVEHLLRLREVQDRTAEKHGVGVFWSFICWTFQPGNTPLVAEGLHNGVATSSYEYLRTLAVARIFLDNIPNIQASWVTQGVRIGQISLDCGCNDFGGTMMEENVVAAAGTIYHATPADAIRLIRDAGKIPARRNTFYEILETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC8</accession>
    <name>Q67RC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipopolysaccharide N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194913.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH780"/>
    <dbReference type="EnsemblBacteria" id="BAD39765">
        <property type="protein sequence ID" value="BAD39765"/>
        <property type="gene ID" value="STH780"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH780"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_38_2_9"/>
    <dbReference type="OMA" id="SVEICIL"/>
    <dbReference type="OrthoDB" id="694191at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="21"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="188"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="375" mass="40811" checksum="6EFF0B8F76AE1993" modified="2004-10-11" version="1">MRIALVCTEKLPVPPVRGGAIQTYIDGVLPYLAARHDVTVVGRADPALPPAEEAGGARFVRVAADDGPEAYADRVAAFLAAERWDAVEVFNRPAFVERIARAAPGARLILSLHNAMLAPDRIDPARARRILGMVDRVVTISDFIRRTAVRIYPEFAAKFRTIYSGVDLDRFRPGPCPAAAALRAALGIGGRTVVLSVSRLSPKKGVHLLLEAMAHVARTHPGAVLVQVGSRWYGRNDPDDYVRDLARRAAELGDAVRMAGYVPYGEVDGYFRMADVFVCASQWEEPLARVHYEAMACGLPIVTTDRGGNAEVVAEGRGGLIVRPHDRPEAFAAAIRTLLDDPALRRRMGAENRRLAEARFGWDRVAAELLAVLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI7</accession>
    <name>Q67RI7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH721"/>
    <dbReference type="EnsemblBacteria" id="BAD39706">
        <property type="protein sequence ID" value="BAD39706"/>
        <property type="gene ID" value="STH721"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH721"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031960_9_0_9"/>
    <dbReference type="OMA" id="ERWTETI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="1">
        <location>
            <begin position="15"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="28835" checksum="DF9E55A87A7ECB15" modified="2004-10-11" version="1">MADFPGEYSVALEDLLTGQRWLHNADRLYHPASTVKVPVALYALEQYRAGKIGWGDLIEYTEADVEPPLTGAFEAAEFGDLYPVQDLVHWALAYSDNVAVNMLGRRLGWGNIERWTETIGGRLTHEDRLPRASALSVLQWWRHLHVLSLEDPERASLIVRPLLAATYRGRITAGLPDGVPHMHKHGSYEGSYHDSAIVYATRPYILVVLTEGAPLEEADAAIARLSAEIYRVMTASHSTPLVPVWSADALQTFVGPRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN9</accession>
    <name>Q67RN9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194802.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH669"/>
    <dbReference type="EnsemblBacteria" id="BAD39654">
        <property type="protein sequence ID" value="BAD39654"/>
        <property type="gene ID" value="STH669"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH669"/>
    <dbReference type="eggNOG" id="ENOG5030BEK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080982_0_2_9"/>
    <dbReference type="OMA" id="EQAGWPT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010179">
        <property type="entry name" value="CRISPR-assoc_prot_Cse3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08798">
        <property type="entry name" value="CRISPR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01101">
        <property type="entry name" value="CRISPR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01907">
        <property type="entry name" value="casE_Cse3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24968" checksum="18225564D0CEB237" modified="2004-10-11" version="1">MYLSLLRLNPASAAVQRDLRDVQALHQRVMSAFPDVLDPEVEARAYFGVLYRLELNRYSGQVLLYVQSRVEPDWGRLPAGYLTPADGLPNPAVKRVDEAYARIREGRVLRFRLRANPTRKIDTKSGPNGEKRNGRRVPLSGLDAQLGWMERKAREHGFELLEATVAAAGASERVRSYTTGRTFQGVLFEGRLVVRDAGRFREALERGIGPGKAYGYGLLSVGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RR2</accession>
    <name>Q67RR2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">1-pyrroline-5-carboxylate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH646</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194779.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH646"/>
    <dbReference type="EnsemblBacteria" id="BAD39631">
        <property type="protein sequence ID" value="BAD39631"/>
        <property type="gene ID" value="STH646"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH646"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_9"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07124">
        <property type="entry name" value="ALDH_PutA-P5CDH-RocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005932">
        <property type="entry name" value="RocA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01237">
        <property type="entry name" value="D1pyr5carbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="49"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="514" mass="55412" checksum="24813D077B1C2C48" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS5</accession>
    <name>Q67RS5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH633</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194766.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH633"/>
    <dbReference type="EnsemblBacteria" id="BAD39618">
        <property type="protein sequence ID" value="BAD39618"/>
        <property type="gene ID" value="STH633"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH633"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_7_9"/>
    <dbReference type="OMA" id="RSWQTVR"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="58"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="388"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="421"/>
            <end position="428"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="630" mass="71397" checksum="C3B2297D8859D7BB" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S22</accession>
    <name>Q67S22_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39521">
        <property type="protein sequence ID" value="BAD39521"/>
        <property type="gene ID" value="STH536"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH536"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523652_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269349" evidence="1">
        <location>
            <begin position="23"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="520" mass="56839" checksum="1C076F08B9567D6F" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S31</accession>
    <name>Q67S31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194661.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH527"/>
    <dbReference type="EnsemblBacteria" id="BAD39512">
        <property type="protein sequence ID" value="BAD39512"/>
        <property type="gene ID" value="STH527"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH527"/>
    <dbReference type="HOGENOM" id="CLU_161222_2_1_9"/>
    <dbReference type="OMA" id="EMVVENH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022476">
        <property type="entry name" value="Spore_YabP/YqfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022477">
        <property type="entry name" value="Spore_YqfC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07873">
        <property type="entry name" value="YabP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02856">
        <property type="entry name" value="spore_yqfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="10760" checksum="CFA89F5BE51B8E69" modified="2004-10-11" version="1">MQQRRRTLGERLVSLLELPGDAVLDVPRAVLIGSMELVVENHRGLVEYRPERVVLRMPEGKMTVDGADLRIGFLSPDQAVILGRIDGLRYAPPEGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S55</accession>
    <name>Q67S55_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">grpE</name>
        <name evidence="8" type="ordered locus">STH503</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the GrpE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194637.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH503"/>
    <dbReference type="EnsemblBacteria" id="BAD39488">
        <property type="protein sequence ID" value="BAD39488"/>
        <property type="gene ID" value="STH503"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH503"/>
    <dbReference type="eggNOG" id="COG0576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057217_5_0_9"/>
    <dbReference type="OMA" id="YAYEKIA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="67"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="22302" checksum="03C04DFD06CAE1A0" modified="2004-10-11" version="1">MKKSPQAEDPPEQQTGEAEAVPAQPADAAGGAGAESGAPEAGADSEAPAEAAVDGAPGEAEPEADRVAELMDRLIRLQADFENYRRRVQREKEEIAQYGTQRLLINLLPVLDNLERALATPPNPGDERLRQGVELTARSFLEVLAKEGVKPIEAVGQPFDPHLHEAVMTGDDPDKEEGIVLEEFRKGYMLGDRVIRASMVKVNTRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB4</accession>
    <name>SYL_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">STH444</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194578.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SB4"/>
    <dbReference type="SMR" id="Q67SB4"/>
    <dbReference type="STRING" id="292459.STH444"/>
    <dbReference type="EnsemblBacteria" id="BAD39429">
        <property type="protein sequence ID" value="BAD39429"/>
        <property type="gene ID" value="STH444"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH444"/>
    <dbReference type="eggNOG" id="COG0495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_9"/>
    <dbReference type="OMA" id="TFMVLAP"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152101" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="588"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="861" mass="97335" checksum="CF32ACCC54E5F2F9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD8</accession>
    <name>Q67SD8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1540 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194554.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39405">
        <property type="protein sequence ID" value="BAD39405"/>
        <property type="gene ID" value="STH420"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH420"/>
    <dbReference type="HOGENOM" id="CLU_175500_1_0_9"/>
    <dbReference type="OrthoDB" id="2076861at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011437">
        <property type="entry name" value="DUF1540"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07561">
        <property type="entry name" value="DUF1540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1540" evidence="1">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8257" checksum="14F4AB532244071C" modified="2004-10-11" version="1">MASQQIRCTVSSCYYYAAGDNCAAEEIMVRADPAALGKSGMEVGDIGGEARQSNHTLCETFIPHQQGPKPGIRRIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF5</accession>
    <name>Q67SF5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH403</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194537.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH403"/>
    <dbReference type="EnsemblBacteria" id="BAD39388">
        <property type="protein sequence ID" value="BAD39388"/>
        <property type="gene ID" value="STH403"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH403"/>
    <dbReference type="HOGENOM" id="CLU_828817_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="37492" checksum="A6CFEE29486ACBB6" modified="2004-10-11" version="1">MSGRSASGRRCEAGYASVYVALVVSLVLVPLTLIVIDFTTLAYWRAKLQGTADALAIGAVLETREFHIPIPTEFVGYFPVNGFLINALHLSNLKPGGVHTRVEPKLERLVELNRDRMGAQVEVRAGDARVLPAGNFLSPFMYVHIPVEAEVPLLTPLLGTLLGSDHPNRVTLRAESCAAAWYRPDAWVHRWWDADPETLVKTIDAVINVTDEPQKYYRLINCVDEAADVLSLLEIVAREHLARDPEAREFLEQWMGNRPQPREMQRARKGLERLPERCGPDDPCVDGSRESIEEWARSTVERRRAEEEEAKKREEAARRAAGKDAERNAAGGDDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN9</accession>
    <name>Q67SN9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase_S9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH319"/>
    <dbReference type="EnsemblBacteria" id="BAD39304">
        <property type="protein sequence ID" value="BAD39304"/>
        <property type="gene ID" value="STH319"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH319"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2720844_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="1">
        <location>
            <begin position="10"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="8294" checksum="601BE458B8B54C60" modified="2004-10-11" version="1">MVEDPWSPEGVQALWKISPIAYVKNVKTPISLMHSEFDYRCPIEQAEQFYMAIKFYKKAPTELVATRAPTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR3</accession>
    <name>Q67SR3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194430.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH295"/>
    <dbReference type="EnsemblBacteria" id="BAD39280">
        <property type="protein sequence ID" value="BAD39280"/>
        <property type="gene ID" value="STH295"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH295"/>
    <dbReference type="HOGENOM" id="CLU_2604748_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="79" mass="8705" checksum="EE25463BB6D20625" modified="2004-10-11" version="1">MPSGGEHQGRDEWPVQHLLQFLRFIATVALTILIIAVLGLALGPKDAGTVTLYALVIGLNALIALLYWPVVRFVQRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY1</accession>
    <name>Q67SY1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194362.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39212">
        <property type="protein sequence ID" value="BAD39212"/>
        <property type="gene ID" value="STH227"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH227"/>
    <dbReference type="HOGENOM" id="CLU_1440385_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269662" evidence="2">
        <location>
            <begin position="26"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="188" mass="20424" checksum="7CCECCD28EFCF368" modified="2004-10-11" version="1">MRRPWLRAAALILSTALILSGCGSADNPPPSPPPQTEPKPTPNPTPPETPETPSPPADAPEDPAEPEPDVPALPGDTEALREALRVQSPITYTIVLAHDAPREMDKTAYLDQMLAEQGYPGKNEILLVLFPADNYNIRFAMGSLVFDRRITLQQMLELVQSQYLTRSRQGDPAGGLAALINAINERAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T30</accession>
    <name>Q67T30_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39163">
        <property type="protein sequence ID" value="BAD39163"/>
        <property type="gene ID" value="STH178"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH178"/>
    <dbReference type="HOGENOM" id="CLU_1577699_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="18187" checksum="92A2DC965997543F" modified="2004-10-11" version="1">MRWEVCSMPQWLMRAVEPIVAHGLREMRTEGADREHILREVALMSALVGTGMPAVQAIRLVEAMEPQLLGMPRGEAREPEHAGYPDMGGWGMGFPGMGFPGMGYPGMGYPGMGYPGMGYPGMGYPGMGGWGKDGFGKGMMMGPWGKMPGTGPMGWPQGGQNYMQGTQPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T37</accession>
    <name>UVRC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein C</fullName>
            <shortName evidence="1">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrC</name>
        <name type="ordered locus">STH171</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194306.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T37"/>
    <dbReference type="SMR" id="Q67T37"/>
    <dbReference type="STRING" id="292459.STH171"/>
    <dbReference type="EnsemblBacteria" id="BAD39156">
        <property type="protein sequence ID" value="BAD39156"/>
        <property type="gene ID" value="STH171"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH171"/>
    <dbReference type="eggNOG" id="COG0322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014841_3_2_9"/>
    <dbReference type="OMA" id="HIECFDN"/>
    <dbReference type="OrthoDB" id="1036075at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000227483" description="UvrABC system protein C">
        <location>
            <begin position="1"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="12"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <sequence length="629" mass="71995" checksum="C2440ADA97467B76" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T66</accession>
    <name>Q67T66_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carboxy-terminal processing protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH142"/>
    <dbReference type="EnsemblBacteria" id="BAD39127">
        <property type="protein sequence ID" value="BAD39127"/>
        <property type="gene ID" value="STH142"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH142"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017295_3_2_9"/>
    <dbReference type="OMA" id="TFNQVDW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="129"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="420" mass="45089" checksum="CAB295808A89A5AE" modified="2004-10-11" version="1">MFATERMTLNMDERRTRFGLSLLLVAVTGVVSYALGALGWPPALQESMSQLRDRLPVAGSSLSGLQVERLEEVMRAIQGQYVEEVDVEALNVGALRGMVEALGDRYSTFFTPEEYRSFVEGFEPTFSGIGVTVEISQQTGLLTVVSPIKGSPGAKAGLRTGDAIIQVDGRDITGMSLNEAVALIKGPKGTQVRLLVKREGEPEPLEFVITRDTITVPVLDYRMIDQEAGIGYIQLFEFSKKGAASQVKEAIAELRSQGMTRLIFDVRQNPGGLLDEVVEIASFFLPTGDPVVHIVERAKEPRALTAKEAEKWTGPLVVLVDGGSASASEILAGAVKDAGVGTLIGAKTFGKGSVQTFWRLQDGSGIKLTTAKYLTAGGHSIDGQGVEPDIVVGNPNRIIPGEPGDPQLEAAVRHIREMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T68</accession>
    <name>Q67T68_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Cell division protein FtsX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ftsX</name>
        <name evidence="11" type="ordered locus">STH140</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ABC-4 integral membrane protein family. FtsX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194275.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH140"/>
    <dbReference type="EnsemblBacteria" id="BAD39125">
        <property type="protein sequence ID" value="BAD39125"/>
        <property type="gene ID" value="STH140"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH140"/>
    <dbReference type="eggNOG" id="COG2177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073546_2_2_9"/>
    <dbReference type="OMA" id="ETGIMRL"/>
    <dbReference type="OrthoDB" id="1653234at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004513">
        <property type="entry name" value="ABC_transpt_FtsX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040690">
        <property type="entry name" value="FtsX_ECD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47755">
        <property type="entry name" value="PTHR47755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18075">
        <property type="entry name" value="FtsX_ECD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003097">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX_ECD" evidence="10">
        <location>
            <begin position="59"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR003097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="307" mass="33204" checksum="11C034B114837AB8" modified="2004-10-11" version="1">MSLRGWAAALRDALHNMARGNLMSLASMLSVAVTLLVLAVVALLAVNLEQMAASAESQVEIKGYLCTARRDEVKCDQRDLTEEEIQAILSRIRSMPNVQEVTFLSRHEALEQMKRADPAQAAILSGYEGDENPLSDEIYVKVTDVQLVAEVAEQIQAMPGVAKVDYGKEIVADLLAFTRAVRIGGLGLVLLLLFATVLTLSNTIRLSVYARGREVSIMKLVGATDWYIRRPFVLEGMLLGTVGAGLASGVTAWGYIRLAPAIQQTIPFLPVVQPGAVLQDLTLALLGLGAVLGALASYFSLHRYLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T74</accession>
    <name>Q67T74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH134"/>
    <dbReference type="EnsemblBacteria" id="BAD39119">
        <property type="protein sequence ID" value="BAD39119"/>
        <property type="gene ID" value="STH134"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH134"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1546796_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="18290" checksum="0BC17ECCF390C76E" modified="2004-10-11" version="1">MAEAIGALLFGLALAESDHRERVEHLILPFRDFFGALFFFSFGLSIDPVGLGGAVVPAVIAAAVSMVGNFAAGMLAGRTSGLSHKASANIGLTIVARGEFSIVMANLGVAGGLLPVLQPFAALYVLILAVLGPVLAKHSTAIYDLLSRVFRWQRPKPRRRKITIPEAAQEAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD1</accession>
    <name>Q67TD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribose 5-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH77</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LacAB/RpiB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194212.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH77"/>
    <dbReference type="EnsemblBacteria" id="BAD39062">
        <property type="protein sequence ID" value="BAD39062"/>
        <property type="gene ID" value="STH77"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH77"/>
    <dbReference type="eggNOG" id="COG0698">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091396_4_1_9"/>
    <dbReference type="OMA" id="DYPPFCL"/>
    <dbReference type="OrthoDB" id="1346802at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016861">
        <property type="term" value="F:intramolecular oxidoreductase activity, interconverting aldoses and ketoses"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004785">
        <property type="entry name" value="RpiB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003500">
        <property type="entry name" value="RpiB_LacA_LacB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036569">
        <property type="entry name" value="RpiB_LacA_LacB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30345">
        <property type="entry name" value="PTHR30345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02502">
        <property type="entry name" value="LacAB_rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005384">
        <property type="entry name" value="RpiB_LacA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89623">
        <property type="entry name" value="SSF89623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01120">
        <property type="entry name" value="rpiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00689">
        <property type="entry name" value="rpiB_lacA_lacB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="3">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005384-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="15370" checksum="7B241333A4660963" modified="2004-10-11" version="1">MKVAVASDHGGIDLKATVISVLKSLGCEVADLGTHDRSSCDYPDYAQKVAEGVAAGEYQRGVLICGTGIGMSIAANKVAGVRAALCNEIYSARMARAHNDANVLCLGARVVGSGVAEEIVRAFFTTEHEGGRHARRVEKIRLLEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF6</accession>
    <name>Q67TF6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH52</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH52"/>
    <dbReference type="EnsemblBacteria" id="BAD39037">
        <property type="protein sequence ID" value="BAD39037"/>
        <property type="gene ID" value="STH52"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH52"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_7_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="11097" checksum="E94F1EAFBECAAFAF" modified="2004-10-11" version="1">MPLQTTIERLREMRLRGMADALVRQQQQPELQSLSFEERLELLVDQEWLDRQNRRLARLLKEVKFRVAACMEDVDYQHPRGLDRTVMRSLAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH1</accession>
    <name>Q67TH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lysine 5,6-aminomutase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH37</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194172.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH37"/>
    <dbReference type="EnsemblBacteria" id="BAD39022">
        <property type="protein sequence ID" value="BAD39022"/>
        <property type="gene ID" value="STH37"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH37"/>
    <dbReference type="eggNOG" id="COG0274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_517447_0_0_9"/>
    <dbReference type="OMA" id="SGLCMPE"/>
    <dbReference type="OrthoDB" id="346908at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016176">
        <property type="entry name" value="Cbl-dep_enz_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015130">
        <property type="entry name" value="Lys-AminoMut_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037086">
        <property type="entry name" value="Lys-AminoMut_asu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09043">
        <property type="entry name" value="Lys-AminoMut_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51703">
        <property type="entry name" value="SSF51703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lys-AminoMut_A" evidence="1">
        <location>
            <begin position="5"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="520" mass="57451" checksum="437BC91D11340910" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JA1</accession>
    <name>Q67JA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3268"/>
    <dbReference type="EnsemblBacteria" id="BAD42249">
        <property type="protein sequence ID" value="BAD42249"/>
        <property type="gene ID" value="STH3268"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3268"/>
    <dbReference type="HOGENOM" id="CLU_2810900_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="67" mass="7206" checksum="AEB14517ADDAC1AE" modified="2004-10-11" version="1">MYTGFPRPVENSRSPKRTRGAALFVPTAGLSPGIVDNLWPTWGDAVDSFRATNRKNLHVQTAGPGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JE3</accession>
    <name>Q67JE3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42207">
        <property type="protein sequence ID" value="BAD42207"/>
        <property type="gene ID" value="STH3225"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3225"/>
    <dbReference type="HOGENOM" id="CLU_2169792_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="110" mass="12211" checksum="1B865203BE721678" modified="2004-10-11" version="1">MFSGFCGLVSTCRRDAAGLSVWPLRPSGEMRSASKPLSLVRSRFSWRRNVRWAEAVGCTFRLESDAWITMFEFTGGRVKSRRPSRTGTVLSSSTCFSATGAASPRHRESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI3</accession>
    <name>Q67JI3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate-nucleotide diphosphorylase (carboxylating)</fullName>
            <ecNumber evidence="4">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH3185</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197298.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3185"/>
    <dbReference type="EnsemblBacteria" id="BAD42167">
        <property type="protein sequence ID" value="BAD42167"/>
        <property type="gene ID" value="STH3185"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3185"/>
    <dbReference type="eggNOG" id="COG0157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039622_0_1_9"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="OrthoDB" id="1718114at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="11">
        <location>
            <begin position="20"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="10">
        <location>
            <begin position="111"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD42167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="29661" checksum="259AA711302FE792" modified="2004-10-11" version="1">MTLNPVAVEELVRRALLEDVGPGDLATSAVVPEDARCTATIVAGAAGVLCGHPVAQAVFRAVDPELVYERLVPEGGAVDDGVPVASVSGPARPILTAERVAVSLLQRMSGIATATRQLADAIKYYHARLVAGWETVPDLRVIAQYAVRIGGGQVQPYGPTGGILVRAGHLALAGGVREAVVAARKGAPPTSRIAVEVVSLEQVQEGVDGGADILVLTGMDPETVKRAVEMVAGRAALEVCGSADPAHLTELAKTGVDIISVCDLAHAARPLPFRLSFGDPKALEKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL2</accession>
    <name>Q67JL2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42138">
        <property type="protein sequence ID" value="BAD42138"/>
        <property type="gene ID" value="STH3156"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3156"/>
    <dbReference type="HOGENOM" id="CLU_2620761_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="78" mass="8322" checksum="5A4A57CA51692BBC" modified="2004-10-11" version="1">MPMPRPLHDLRQPQIGPTAGDLGQSAFAANLRDVDGPDAGPQIEATVRPWEAPEPRNPPPGQRRPSGPALRLGADVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP3</accession>
    <name>Q67JP3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197238.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3125"/>
    <dbReference type="EnsemblBacteria" id="BAD42107">
        <property type="protein sequence ID" value="BAD42107"/>
        <property type="gene ID" value="STH3125"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3125"/>
    <dbReference type="eggNOG" id="COG1329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048259_1_1_9"/>
    <dbReference type="OMA" id="DMKVMIP"/>
    <dbReference type="OrthoDB" id="1942592at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042215">
        <property type="entry name" value="CarD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CarD_TRCF" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="18148" checksum="029A6E09063B1514" modified="2004-10-11" version="1">MFQVGDKVVYPMHGAGIIEAIEDREVLGERKRYYVLRLPVADMQVLVPCDGPQSTGLRAVMSEQVFQQVLEVLRSRPTQAEKNWNHRYRMNMEKIRSGDVLALAEVVRTLSQREREKGLSTGERKMLDSARQILLSEIVLIRGLELEQASSLLDRVLAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT2</accession>
    <name>RL7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplL</name>
        <name type="ordered locus">STH3086</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197201.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JT2"/>
    <dbReference type="SMR" id="Q67JT2"/>
    <dbReference type="STRING" id="292459.STH3086"/>
    <dbReference type="PRIDE" id="Q67JT2"/>
    <dbReference type="EnsemblBacteria" id="BAD42068">
        <property type="protein sequence ID" value="BAD42068"/>
        <property type="gene ID" value="STH3086"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3086"/>
    <dbReference type="eggNOG" id="COG0222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086499_3_2_9"/>
    <dbReference type="OMA" id="LEDKWGV"/>
    <dbReference type="OrthoDB" id="1822695at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_L7/L12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000243507" description="50S ribosomal protein L7/L12">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <sequence length="127" mass="13006" checksum="0FE49D53059CB152" modified="2004-10-11" version="1">MSKAAEVIEIIKGMTALELKELKELFEETFGVTAAAPVAVAAAPAAGAAAAAPAAEEKTEFDVILKNPGANKIAVIKVVRELTGLGLKEAKDLVDGAPKPVKEKVSKADADAAAEKLKEAGAEVEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU4</accession>
    <name>RL4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplD</name>
        <name type="ordered locus">STH3074</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197189.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU4"/>
    <dbReference type="SMR" id="Q67JU4"/>
    <dbReference type="STRING" id="292459.STH3074"/>
    <dbReference type="EnsemblBacteria" id="BAD42056">
        <property type="protein sequence ID" value="BAD42056"/>
        <property type="gene ID" value="STH3074"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3074"/>
    <dbReference type="eggNOG" id="COG0088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_5_2_9"/>
    <dbReference type="OMA" id="PQVHILE"/>
    <dbReference type="OrthoDB" id="1572673at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000242447" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="207" mass="22519" checksum="FF5D9209623226D8" modified="2004-10-11" version="1">MPKVAVYNKEGATVGEITLSDAVFGAEVNPGLLHEVVQMYLANKRQGTADTKTRAEVSGGGRKPWRQKGTGRARHGSIRSPLWRKGGIVFGPHPREYGWSMPKKARRAALRQALSAKVKSGELIVVDKFELEAPKTREVATLLKNLKVDGSAFIVTAQEDVNIYKSARNIPGVRVNAARNLNAYDVLAASKLVFTQDAVAKVEEVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY5</accession>
    <name>Q67JY5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anti-sigma-W factor RsiW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3032</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-associated anti-sigma factor (ZAS) superfamily. Anti-sigma-W factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197147.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3032"/>
    <dbReference type="EnsemblBacteria" id="BAD42015">
        <property type="protein sequence ID" value="BAD42015"/>
        <property type="gene ID" value="STH3032"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3032"/>
    <dbReference type="HOGENOM" id="CLU_1874387_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-HC2" evidence="3">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="15223" checksum="B8AD8940B10045BF" modified="2004-10-11" version="1">MATMRCDEELVQLYVDGTLHPAEAALVEAHLRQCRACRRLAGFYKSLYWDLGRTATLAPDPAQDPDALAEVLLAEHRRHRQPEAPPASAWAFSTLWLTANPAFARPARTLTALGHRGAEGLVRTLKRRWRRRKGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY8</accession>
    <name>Q67JY8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42012">
        <property type="protein sequence ID" value="BAD42012"/>
        <property type="gene ID" value="STH3029"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3029"/>
    <dbReference type="HOGENOM" id="CLU_2014106_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13326" checksum="2E6F35DFAB755D1D" modified="2004-10-11" version="1">MVFNPLLLSYAIPTMGLDLEPGGQRVLMDGVFFIGAIVAVLGVFLLRGASRPHLNRELVKLVIWVELIAGLVLNLYLALRGYGHPLALVAFALLHGAIALVGRHALVTCRRHLTAVKPLKRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K52</accession>
    <name>Q67K52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2963"/>
    <dbReference type="EnsemblBacteria" id="BAD41946">
        <property type="protein sequence ID" value="BAD41946"/>
        <property type="gene ID" value="STH2963"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2963"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_3_2_9"/>
    <dbReference type="OMA" id="VFPLFQH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="196" mass="21357" checksum="C204BA77BC2F0A03" modified="2004-10-11" version="1">MCRGGAWMSLGHRIRQTRTQKGITLQELAERSGLSKGFLCQLENDKASPSLQALDRLSAGLGVPVALLLISQEERAHVVRANARQGYSMNGEGLSVQLLSAPGRSLKMMLLELAPGAATGGDNHVHEGEECHLVLQGTVRYRQGDEELILNEGDSLHWNGYIPHRVENCGDQPARILCVTTGAMEHMLESECNECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K64</accession>
    <name>Q67K64_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197067.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2951"/>
    <dbReference type="EnsemblBacteria" id="BAD41934">
        <property type="protein sequence ID" value="BAD41934"/>
        <property type="gene ID" value="STH2951"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2951"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_2_0_9"/>
    <dbReference type="OMA" id="YALSIDM"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018833">
        <property type="entry name" value="DUF2437"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10370">
        <property type="entry name" value="DUF2437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2437" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="56"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="27150" checksum="2DE02A51F4211D1F" modified="2004-10-11" version="1">MRYVRFLTEDAPPRWGRQEGDVVHELDGAPYEGGQPTGRTVPLQAVQLLAPVTPSKIVCIGRNYRDHAAELGNAAPERPMFFLKAPSALIGPGEAIRLPDPDATVHWEAELAVVIGRRMKDVAPADALSYVFGYTIANDVSHRDAQKADGAFGFGRGKSYDTFCPCGPAVVTEGIDPADALITLTCNEEVRQSDSTGLMIHPVPELLSYLSQIMTLMPGDLVLTGTPKGVGAMKPGDLIEIHIPGIGTLANPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB1</accession>
    <name>Q67KB1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">GTP 3',8-cyclase</fullName>
            <ecNumber evidence="3 12">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">moaA</name>
        <name evidence="14" type="ordered locus">STH2904</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="3 12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197021.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2904"/>
    <dbReference type="EnsemblBacteria" id="BAD41887">
        <property type="protein sequence ID" value="BAD41887"/>
        <property type="gene ID" value="STH2904"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2904"/>
    <dbReference type="eggNOG" id="COG2896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_9"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="OrthoDB" id="1199289at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="260"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD41887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="329" mass="36665" checksum="81FE21BA14E4B8DE" modified="2004-10-11" version="1">MIDQWGRKVNYMRISVTDRCNLRCVYCMPLTGVKLGPHDEYLRYEEILRVVKAAVGLGIDRIRVTGGEPLVRQGIVEFLAQLKPLGVTDLSLSTNGLLFAPMAKDLKAAGVDRVNISLDTLRPDRFTKIARLSGSPAQVLEAAEAALELGMEPVKLNMVVIRGWNDDEIGDMARLTLDRPIHVRYIEVMPFSEGYQFTWENLVPAAEMRERLLDEFGDLEPVRGGVRGNGPAKYWRLPGAKGTVGFISAVTECFCAECNRIRLSADGKVNPCLGHVHEVDLKPVIRDPEKTDDDLRNALADAILRKPREHNFDDAESDYVLRVMHGIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF0</accession>
    <name>Q67KF0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2863</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2863"/>
    <dbReference type="EnsemblBacteria" id="BAD41848">
        <property type="protein sequence ID" value="BAD41848"/>
        <property type="gene ID" value="STH2863"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2863"/>
    <dbReference type="eggNOG" id="COG2108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_620840_0_0_9"/>
    <dbReference type="OMA" id="NLLEFCY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="PTHR43288:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="51"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="45130" checksum="48A192BEB46A5808" modified="2004-10-11" version="1">MQQAGLPLEPPGRNGEAQDLRAELRARGASFRNADHSIHLGPLSPACEACRMGVGTATFFASLKCHRRCFYCFNPNQEGYDFFQEHRRDLGAELAELAEGGFQVSHVALTGGEPLLHPEEAVTFFRTAKQLFPGVHTRLYTSGDHLGEALVRQLAEAGLDEVRVSVRVDDGPAGRRHTYSRLALAREHIPAVMVETPVLPGTLDAMTEMLLELDRIGIFGVNLLEFCFPFHNAEAFRERGYQVKNPPYEVPYDYWYAGGLPVAGSEVDALRLVKFALDQGLSLGVHYCSLENKHSGQIFRQNSTASLPATACFSARDFFIRSAKVFGRDALRSRRRFDALGYRGYAVDPARDFLEFHPAWIPQLRGVADEVALCTYVAEERPEGPVLRELKISVTTPGTFDPETDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KJ8</accession>
    <name>Q67KJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0236 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196934.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2815"/>
    <dbReference type="EnsemblBacteria" id="BAD41800">
        <property type="protein sequence ID" value="BAD41800"/>
        <property type="gene ID" value="STH2815"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2815"/>
    <dbReference type="eggNOG" id="ENOG502ZACA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040782_0_0_9"/>
    <dbReference type="OMA" id="MHEGWEA"/>
    <dbReference type="OrthoDB" id="683481at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009620">
        <property type="entry name" value="UPF0236"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06782">
        <property type="entry name" value="UPF0236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="38384" checksum="64AEE1B7F85CB674" modified="2004-10-11" version="1">MFKVADSVTSFKDLVQWLLEKLAADFCQQVETVLAEIENQREREESLKGWESIGPRERTLVSLFGMEIRFRPRGYRQRRPDGSWAYRYPLDEMLGLLPEERFCPLVQELAVELAARMSFREAATVLREHLRVPVSHQEVHRWLQEAGQERDRQFREEVQAVFERGQAPESEGRGAEMVVIEADGMYIPLQRERQRVAELKLAVMHEGWEAESPAGERFRLMNKAVWAGYMQAEAFWERGDICFAQRYDPEKVGRVIVNGDGAEWIKEARKHFSNVELYLDPFHRNRAIQEALGFAPDLVRRALNAVRSGDLKGLGRVLNEAVAVARGEDQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL5</accession>
    <name>Q67KL5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative sugar transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2798</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2798"/>
    <dbReference type="EnsemblBacteria" id="BAD41783">
        <property type="protein sequence ID" value="BAD41783"/>
        <property type="gene ID" value="STH2798"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2798"/>
    <dbReference type="eggNOG" id="COG2211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038661_0_0_9"/>
    <dbReference type="OMA" id="PMVVDMT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="7"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="402" mass="44996" checksum="D799BEE3E2D34CD5" modified="2004-10-11" version="1">MRLNWKNTFIIGLGFFGVSLVWPLYNAYMPIFYARYIEAKATIGYLMTIDNWLALTLTPLVGFLSDRTRTRFGRRIPYLLVFAPLSALFLMLIPVGWETSLWLLLGAAVLMNLSMACWRSPIVALMPDVTPPPLRSKANGIINFMGGLAYIVAMFGGAILYRMYPGLPFVASALLLLGITAIFYFFIREPEHSTEKSERFQFALIRDRSAIFMLLAIFCWFVAYNSLETWVTTYGREHLGMHEADVAGLLTFSGGAFLLFAIPSGFLADGIGRFKGLGRKRTILGGLLLMILAFFLLDLQANLKSAWYLFALVGFAWSWVSINSYPMITQMAGPGQIGAYTGMYYLFSSMANIAGPPLFGWVFDHYGYRFFFPLGILFMALAALCMLLVTKGEKEPAAEARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM8</accession>
    <name>Q67KM8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AhpC-TSA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2785"/>
    <dbReference type="EnsemblBacteria" id="BAD41770">
        <property type="protein sequence ID" value="BAD41770"/>
        <property type="gene ID" value="STH2785"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2785"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2738575_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AhpC-TSA" evidence="1">
        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="71" mass="7586" checksum="769CF1980CE9DCFA" modified="2004-10-11" version="1">MALIAAGLEESGGLRRIADQKETGVKAPMVQPGEVAPDFTLESTEGPFTLSALRGRKRALIIFYPKDNTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR0</accession>
    <name>Q67KR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2752</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2752"/>
    <dbReference type="EnsemblBacteria" id="BAD41737">
        <property type="protein sequence ID" value="BAD41737"/>
        <property type="gene ID" value="STH2752"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2752"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="IDDLHHK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="15"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="370" mass="40780" checksum="B8CEBCA9EBF1A2E7" modified="2004-10-11" version="1">MNREGESGMGEAQSVTLERVSKSFNGTVLTVKNVDLHIAGGEFFTLLGPSGCGKTTTLRMIAGFYYPTSGRILFGDRDVTYMPPNDRNIGMVFQNYALFPHMSVFENVAFGLRVRKVPREEMKRRVSEALALVRLEGMEQRRIDQLSGGQQQRVALARALVIRPALLLLDEPLSNLDAKLRDETRTEIRRIQTESNITAVYVTHDQAEAMAISDRIAVMEGGVVHQVGTPQEIYHRPATRFVATFIGKSNLLEGTLTEVDGTAGVVELPGTGRIRVDLERRNPQVPTAVGSRVVMTVRPEGIQVVAQPEGPNVLTGRVVAAEFTGAYTEYLVKAGESTLVASSADRYGEVRRPGDPIHLYIPPERVYLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR7</accession>
    <name>Q67KR7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2745"/>
    <dbReference type="EnsemblBacteria" id="BAD41730">
        <property type="protein sequence ID" value="BAD41730"/>
        <property type="gene ID" value="STH2745"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2745"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_4_9"/>
    <dbReference type="OMA" id="WEMILHA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="57"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="551" mass="61403" checksum="F942726A6A5CA159" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ0</accession>
    <name>Q67KZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2671"/>
    <dbReference type="EnsemblBacteria" id="BAD41656">
        <property type="protein sequence ID" value="BAD41656"/>
        <property type="gene ID" value="STH2671"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2671"/>
    <dbReference type="HOGENOM" id="CLU_2208716_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="12015" checksum="97484CEED0872909" modified="2004-10-11" version="1">MDVRFSSGQEGFAAHPPNSLRRQTRRSGDMAKGALTQKDLMIWVMNHPNGTKYYYLIEREPFEREGIKKAVHRMGSTGDAVFLYRDQAEAELAKFPPGDEEPEGAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L21</accession>
    <name>Q67L21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase iron-sulfur subunit</fullName>
            <ecNumber evidence="6">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2640</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="6">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2640"/>
    <dbReference type="EnsemblBacteria" id="BAD41625">
        <property type="protein sequence ID" value="BAD41625"/>
        <property type="gene ID" value="STH2640"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2640"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_3_3_9"/>
    <dbReference type="OMA" id="KYDPLVW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102040">
        <property type="term" value="F:fumarate reductase (menaquinone)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 6" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="141"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="28128" checksum="1694DCB7D7EE3446" modified="2004-10-11" version="1">MTRAMKDFKLRVFRYDPEKDEKQHYDTFTVEYRDGMTVLEALLWVFEKKDPSLAFRYSCREAICGSCAMYISGRYALACRVQVKDALEGDTVTVSPLPHMRVIKDLVVDQTKFWENYARVKPWLINDNPPPERERLQSPEDRAKYNMEIDCILCGACFSSCPSGANNEEYLGPHALVWASRFFHDTRDTAKRERLEIVASEYGVFRCHTIFNCVEACPKHINPTEAIQKLKKAAMAYKMGLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L58</accession>
    <name>Q67L58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41588">
        <property type="protein sequence ID" value="BAD41588"/>
        <property type="gene ID" value="STH2603"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2603"/>
    <dbReference type="eggNOG" id="ENOG50330WY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_694284_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="384" mass="43430" checksum="1ECB11A32411E354" modified="2004-10-11" version="1">MHSAQLEYAEHSPARIRALLNRIDPAAVGVETPPQWYAEDVYFEVAYESYGVAVPWAREKGREVRPVDWTADPLEQVNALLWPAVNPEPDPGGSGEERITYEDLLQSWDVPPLLFADTPEWHDAVNRSYANADANPNPYGEAGRRYMLYRNLMIAREIVNMAADHEGKRVVVLIGAAHKPDLDLFLATVPNIVVRQASEWDGATAAEVAAEERRADHLAILWYNLAGTRRIKDGDVDLTRMDRLLAGLEKATPLDPEVRFLRARWHHVRGDTARAVAQYAALAWGQTWEDRPFTFPDVGLYKRMMDWSNAYASMGQTVEYGIDNVFSPVGNLTVRQRVLYELATQEHDPAARERARHELLAEPLNVRQRQQLQALLNPPAAEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF5</accession>
    <name>Q67LF5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196629.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2506"/>
    <dbReference type="EnsemblBacteria" id="BAD41491">
        <property type="protein sequence ID" value="BAD41491"/>
        <property type="gene ID" value="STH2506"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2506"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="YFEGANT"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="10">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="26157" checksum="6D1E6367A98BF89C" modified="2004-10-11" version="1">MAEPLILVVDDEQAIADLIEIHLLAEGYRVRKADNGADALRIVAEEDPDLVVLDIMMPGMDGLEVCRAIRRERNVPILMLSAKSEDVDKILGLKTGADDYLTKPFNPLELVARVKAQLRRYLSLNPASAAAREPGVLAVGGLRIDPLGRSVFKDGREIALTPTEFDILLLLASHPGRVFSSEEIFERVWKEHYFQSNNTVMVHIRRLREKVEDDPSHPTLIRTVWGVGYKIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LF6</accession>
    <name>Q67LF6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH2505</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2505"/>
    <dbReference type="EnsemblBacteria" id="BAD41490">
        <property type="protein sequence ID" value="BAD41490"/>
        <property type="gene ID" value="STH2505"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2505"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_3_9"/>
    <dbReference type="OMA" id="ADAFDHM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269254" description="Histidine kinase" evidence="8">
        <location>
            <begin position="36"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="79"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="146"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="42559" checksum="2B4A93BC969B8788" modified="2004-10-11" version="1">MRSNLAFSLRWRMILTFMASGFLAAVSTALMLAVAANLAGSIPPFNWLLNFLADTVGVWPIVFAVAVVLSFTWYILLSQPMIRYLEALSRAMDEVARGNYDVTVPPRGRDELGLLGENLVSMSRQIKASLQRERQANQARYELITAVSHDLRTPLTSVLGYLQLIDEDRYRDEVELRYYVDMAYEKAKQLKRLIDQLFEFTRTSHGGIVLRPVPINPAELLEQVAEAFVPALQEAGMEYRLRLPDERATVLADPDLLVRVLENLISNAIRYGREGKVVELELEVRRVERAVLLRVANRGNPIPSHQLDRIFDSFYRGEVSRSGRTGGAGLGLAIVRNIVTLHGGTVRAINETDRTVFEVRLPAAGGAEAAGAGLSPGAAVRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG7</accession>
    <name>Q67LG7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196617.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2494"/>
    <dbReference type="EnsemblBacteria" id="BAD41479">
        <property type="protein sequence ID" value="BAD41479"/>
        <property type="gene ID" value="STH2494"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2494"/>
    <dbReference type="HOGENOM" id="CLU_911115_0_0_9"/>
    <dbReference type="OrthoDB" id="1244064at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022025">
        <property type="entry name" value="Amidoligase_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12224">
        <property type="entry name" value="Amidoligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="324" mass="35243" checksum="2861A749DF76E597" modified="2004-10-11" version="1">MWPRTVDWEELRFGVEIEFVGGNPAAVELLPGWVMAFDELQVDDTGEDSGAELKPPPLLWRDREQMRVMLDRLKATGARANWSCGLHVHVGLEPWGQDIVLPLVDEALAHQDALRGLLRTAEDRLIHCPPVVPAMRDRYLAKPERASLVRRGRPQSHRCGINAAAWFDFGTVEIRYPNGSLEYDEVVNTVELCLRFVAAVGAGRLVPPAAAGATGGGAAASGPAASAADAAALAALLGAPADGYPLPRPAPRWHRERAWLEDALVPVLEPLVRSRVPEAEILAIRPVPGGFRVTAEEPGDRRMAFLTRPGPDGWSLEPLESERG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP7</accession>
    <name>Q67LP7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2414"/>
    <dbReference type="EnsemblBacteria" id="BAD41399">
        <property type="protein sequence ID" value="BAD41399"/>
        <property type="gene ID" value="STH2414"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2414"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_862888_0_0_9"/>
    <dbReference type="OMA" id="HMEICKE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="37280" checksum="36E71C95FBC7CA73" modified="2004-10-11" version="1">MNPGSTSERSRKMEARRRAVDARFVEGWMSYIGAVQGVLNAAGMGPWEYWRLMGDTGMAFHLVMHETCCVSSVTAYDWMNTHLAALSRIGVHSEVYGAHPAMPTFDAARRRALVHIRESIDRGIGVVLWGVDSGEFGVVWGYDEEDGVLLTSGCHGPRGNPIPYENLGMSFPGAPELHYQIVLERVPADERQTARSSLRYYVDLMERQGHMGPGYRAGLSAYDNWMRGLQREGFDQFGCRYATFVYAEARACAARYVEHLAEAERALAPAAEAFRRTAAIYGRMMEVLGQDLADPSALDTPVSAEQAAALIPLLREAKESESETVARVAQYLRTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ0</accession>
    <name>Q67LQ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose:sterol glucosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196534.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2411"/>
    <dbReference type="EnsemblBacteria" id="BAD41396">
        <property type="protein sequence ID" value="BAD41396"/>
        <property type="gene ID" value="STH2411"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2411"/>
    <dbReference type="eggNOG" id="COG1819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_8_0_9"/>
    <dbReference type="OMA" id="ICPFFGD"/>
    <dbReference type="OrthoDB" id="1485440at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033072">
        <property type="term" value="P:vancomycin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="1">
        <location>
            <begin position="3"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="44387" checksum="75922337D68777E8" modified="2004-10-11" version="1">MKFAILATGTRGDIQPFVALAQGFSAAGHEAVLAAPRAFESFVRSYGVDFFPMGSDFTALLESPEGKAALRENPIKTMQAVRQTVIPMMRRMLDDAWAAVQGADAIIYHPKALAGVHLAERLRVPCFIGAPVPVVVPTATFPAPAFVSRDLGGFLNRLTYAAVRSGTRPFRKMIDAWRREVLGLGPRREDEFTQGGQPVPVLHAFSRHVVPPPADWPPEAVVTGYWFARRVQVWHPPEALSAFLEAGPPPVYVGFGSVAGLDRDEGTRMALEALSQAGVRGILATGSGDGPRLLSPDVLAIPEAPHDWLFPRVAAVVHHGGAGTVAPGLAAGKPTLVCPATTDQPFWGRVVHQLGVGPAPIPRRQLTAERLAEAVRAATGDPEMQRRAAALGEAIRAEDGVAQAVAEILRRVGALAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV2</accession>
    <name>Q67LV2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2359</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196482.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2359"/>
    <dbReference type="EnsemblBacteria" id="BAD41344">
        <property type="protein sequence ID" value="BAD41344"/>
        <property type="gene ID" value="STH2359"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2359"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="IVMSISQ"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="31770" checksum="0235EE02E4A1E728" modified="2004-10-11" version="1">MQVLSVRNLAKRFGGLDAVRSVSLDVGPGETVAVIGPNGAGKTTLFQLIAGAERPDGGQVYLEGERIDGLPAHVIAAKGIARTFQNGRVYGNLTVLENVLLGAHTRLQAEFGRPAGPLTGVLGAIREVIAAMARPARLRRLEAELVEEAVEILSLFGDRLLPRLHQPAYSLSYANRRRTELARALALRPKILLLDEPTAGMNPTETEEMTEVIRLLQARGHTILLIEHKLDVVSALAHRVVALEAGEVLVAGTPAEVWSDRRLVEAYVGRSPGRRQPPAAAPAPPAQDARARAAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX1</accession>
    <name>Q67LX1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2340"/>
    <dbReference type="EnsemblBacteria" id="BAD41325">
        <property type="protein sequence ID" value="BAD41325"/>
        <property type="gene ID" value="STH2340"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2340"/>
    <dbReference type="eggNOG" id="COG1172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028880_2_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="331" mass="33890" checksum="157B4837A03706D8" modified="2004-10-11" version="1">MEGTELAKDIAVKKRLSFSDVWDKYSTVLILILMILVFGALEPVAFLGGSNLIKIVEQSAITILLACGEFFAILLAGIDLSVSSTMALTGVVTAKLLVAGVHPFLAVVLGAILLGGLIGALNGGLINATGLPPFIITLGTQAILRGLTLTVSSARAVSGVPVTFTKAMGGKLFGVVPTPILIALITALVLTFFTKKTQAGRNLYAIGGNAQSAWFAGINVKRHTLLAFTIAGICAGLAGMVNIARLGAAEPNAGTGYETFAIAAAIIGGTSFFGGVGVVPKVVVGGLIIGVINNGLNMVGVSSYYQQIAMGALIIVAVTLDRFFGASRKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M00</accession>
    <name>Q67M00_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transketolase N-terminal subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196434.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2311"/>
    <dbReference type="EnsemblBacteria" id="BAD41296">
        <property type="protein sequence ID" value="BAD41296"/>
        <property type="gene ID" value="STH2311"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2311"/>
    <dbReference type="eggNOG" id="COG3959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_9"/>
    <dbReference type="OMA" id="MVHAVNS"/>
    <dbReference type="OrthoDB" id="1543182at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="29595" checksum="8DB443B9F29764DF" modified="2004-10-11" version="1">MTAPDVPLLTEIAKRIRVHVIRTVLRLGSGHLGGSLSAAEILSVLYFHTLRIRPEEPDWPGRDRFILSKGHAALAYYAALAERGYFPPDELAGFGALGSRLQGHPDMRRTPGVDMSTGSLGQGLSAGVGMALAARIRGEGWRTYVLLGDGEIQEGQVWEAAMLAGELRLTGLTAILDFNRLSQTRSTTGAHPPDLLPGRWRGFGWEVREVDGHDVSALAAALDRPADGRPLLVVAHTVKGRGVSFMEGRPEWHSHPLTREQAEQALAELGVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M18</accession>
    <name>Q67M18_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196415.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2292"/>
    <dbReference type="REBASE" id="10273">
        <property type="entry name" value="StpMcrBP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41277">
        <property type="protein sequence ID" value="BAD41277"/>
        <property type="gene ID" value="STH2292"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2292"/>
    <dbReference type="eggNOG" id="COG0507">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1401">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_448273_0_0_9"/>
    <dbReference type="OrthoDB" id="489714at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="609" mass="67389" checksum="570890E81B75CFE2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M20</accession>
    <name>Q67M20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2290"/>
    <dbReference type="EnsemblBacteria" id="BAD41275">
        <property type="protein sequence ID" value="BAD41275"/>
        <property type="gene ID" value="STH2290"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2290"/>
    <dbReference type="HOGENOM" id="CLU_1634530_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="18289" checksum="FD7EED6003DA02ED" modified="2004-10-11" version="1">MNHRTGWDGLLRTRLPVRGCRTPSGSRPPIEPNLRTLGGTRMKIHVEVWLAAASLHEKGREVMTTRELQDEVKRLFGDERSGVVTHINGHANAASPKGAGVVYNYLFRVGPGTHRLCRIGEPVHPTRTGRQLWPDQAEVDEQYWPLWRKWVVWQQGGGAVTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB0</accession>
    <name>Q67MB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative phospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2198</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196323.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2198"/>
    <dbReference type="EnsemblBacteria" id="BAD41183">
        <property type="protein sequence ID" value="BAD41183"/>
        <property type="gene ID" value="STH2198"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2198"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_0_0_9"/>
    <dbReference type="OMA" id="VIRPWAD"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="12"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="270"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="343" mass="35739" checksum="E527C28C43540A63" modified="2004-10-11" version="1">MGKCTDGPRVGLALGGGGARGAAHVGVLRALERAGIPIACIAGTSSGAMVGAAYAAGLSPDQIERVFASVRFRDLFRPVWARDGWLDNSPLADTFEREAGRLNAEDLRIPFAATATDADTGEPVTLQCGPLGPILRATTAMPCIVRPVHLDGRRLVDGGVVHKVPVQLARAMGADIVVAVDLSFPYPWRRKKARHPLVFLMRMLEIMEQHLVAAQLDGADVVIRPWADCGTFEFHRYRAQILSGEAAAAKAIPAILACLDRFAAAGRPIPAPATDHAPATDHAPATDHAPAAGRETRPSTGAAAEARPALTPAPVAPAAHPAPAPRPGLLPLPAPGQTQASAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD8</accession>
    <name>Q67MD8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN_MAE_28990 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41155">
        <property type="protein sequence ID" value="BAD41155"/>
        <property type="gene ID" value="STH2170"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2170"/>
    <dbReference type="eggNOG" id="ENOG5032SV9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100981_1_0_9"/>
    <dbReference type="OMA" id="YAHWEGY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040788">
        <property type="entry name" value="HEPN_MAE_28990"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18737">
        <property type="entry name" value="HEPN_MAE_28990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN_MAE_28990" evidence="1">
        <location>
            <begin position="11"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="22386" checksum="8593F9BB5D7CBC25" modified="2004-10-11" version="1">MEACKRDHLRKLWLRAAVPILYAHWEGYIKLAAQAYLAYVASKGLRYCDLQFNFVAVARLGDIQQAARTKQAEKHVQLVESFIRFDTEQARIPTEGIIDTASNLNSEVLSNVLCTIGLPYDEYWRAKAVKIDRSLLGLRNGIAHGERREVTLDEYRELHELVIDCLEYMKRMVEAYCENRWYLRGSALDKRMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD9</accession>
    <name>Q67MD9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196294.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2169"/>
    <dbReference type="EnsemblBacteria" id="BAD41154">
        <property type="protein sequence ID" value="BAD41154"/>
        <property type="gene ID" value="STH2169"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2169"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1293793_0_0_9"/>
    <dbReference type="OMA" id="VMIKKHR"/>
    <dbReference type="OrthoDB" id="1692349at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="23160" checksum="DE8688E985FB58BC" modified="2004-10-11" version="1">MRRLVRTWDRLVRRFLGIRPARPDGVLGYAVRRYRGRGIAVEGGASLRRGDLVLELHLDSRRLAEKTAGQSPHRRILWLRRTLLADLRALARAVESDPALAGAAGLWGMTVLHRGVDALGFAVAEPAAGFVGRLATWYMRGLLAAYHPEATDRVQHRAEALVAREIFLPMDRLRALIGPAPADGEEAFQTVGPARPGRSSGPGGPTASSEPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK6</accession>
    <name>Q67MK6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acylamino-acid-releasing enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2102</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196227.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2102"/>
    <dbReference type="ESTHER" id="symth-q67mk6">
        <property type="family name" value="Prolyl_oligopeptidase_S9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41087">
        <property type="protein sequence ID" value="BAD41087"/>
        <property type="gene ID" value="STH2102"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2102"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_3_2_9"/>
    <dbReference type="OMA" id="IAFTANR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="3">
        <location>
            <begin position="404"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="610" mass="68325" checksum="66C67F1F726CDF34" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT6</accession>
    <name>Q67MT6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0236 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196148.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2022"/>
    <dbReference type="EnsemblBacteria" id="BAD41007">
        <property type="protein sequence ID" value="BAD41007"/>
        <property type="gene ID" value="STH2022"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2022"/>
    <dbReference type="eggNOG" id="ENOG5031FFI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040782_3_0_9"/>
    <dbReference type="OMA" id="ESKRIEY"/>
    <dbReference type="OrthoDB" id="683481at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009620">
        <property type="entry name" value="UPF0236"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06782">
        <property type="entry name" value="UPF0236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="465" mass="53402" checksum="ED152E202A3E1494" modified="2004-10-11" version="1">MEQIRAAVDFSFQEIERLAWQQTLDCFREALVEALSAIDTALYESRDPSRYVYKEMRSRSVVTKFGPITFQRRYYWDREEERFVFLLDEVLGIAKRQRVSDSVRADAVEASVTAGSYRGAAAELERRDCQVFVSHEAIRQWNLQTGRALAAAEKQQQMTLAGTRRVRVLFIEADGFWPARQRGKKAEVRLFVIHEGWIQRGPGSKEYSLVNRRDFVPEPGRDSWEQLSELLESEYDLSETWVIINGDRALWIREGVTWFPKALYQIDRFHLKRELNHVLRHRPQRLEQAHAALEANDAGRLLAVLDEAHKAETDTDRRGKMRRLLADLRMMPESIRDYRIRLQERGVNVEGLRGVGAAEGAVERYSARLRKVGRSWSESGLMAMLQVMAAYYRGALRGAVQYVERALGLESVNAAAEKVRHRVRETVGRGVDAARHARMPILNAGRNNSGGYSKTFRGFAGLVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU6</accession>
    <name>Q67MU6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2012</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2012"/>
    <dbReference type="EnsemblBacteria" id="BAD40997">
        <property type="protein sequence ID" value="BAD40997"/>
        <property type="gene ID" value="STH2012"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2012"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_57_3_9"/>
    <dbReference type="OMA" id="AGRWCER"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="16"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="410" mass="43500" checksum="4626813B6BD8CCCF" modified="2004-10-11" version="1">MTERRRHNEMEPWRRNVAVLWIAVFIAASAWTMVMPFMPVFLSEDLGVGAGVTVWAGLLGAVNSGFSALTAPIWGALGDRWGRKAMMVRAGIFLGSAYVLMAFVRNPYELLGVRVMIGALTGFIPTATALVATTTPERHMGQALSLVATGNQAGSILGPMLGGFLADLIGIRNTMALSGVAVFAATLLVVFAVREQFQRPARQPAHFLSDMRQVLQGGPLIVVLAMSLLLQMSQSAMEPVLIPHIRQLLGSGSPNWMAGVIYSVPGIAFVLAAPSWARLGERWGYTTTVTIGLAGGALFTLPQALVQSGLGMGSFRLSAGLALAAVTPGISALIAASVPREQRGKAFGLNQSAFNVGAMLGPLLGGVLGDRVGIRWVFPATALLLLFAAGWCWSLVRARVRQHRDSQLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV9</accession>
    <name>Q67MV9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1999"/>
    <dbReference type="EnsemblBacteria" id="BAD40984">
        <property type="protein sequence ID" value="BAD40984"/>
        <property type="gene ID" value="STH1999"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1999"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_2_9"/>
    <dbReference type="OMA" id="TIFIGER"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="191" mass="22056" checksum="6DDF46C760AC17A2" modified="2004-10-11" version="1">MIWGEKAGIRPLRPTDAGTLYRFMTDPEVADLLYEEKAGPVPGPALLAFNVWLNQLAARPEWAIVDERERFIGVVRLWRVSERNRSAMLTIFIGERSCWGRGYGTDALRLVLRQAFGPMDLHRVELHVFDFNKRAIRAYEKAGFVHEGVRRQALRRGSRYHDILVMGITRREFYAREQQRAAARDQGASLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX0</accession>
    <name>Q67MX0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">(R)-citramalate synthase</fullName>
            <ecNumber evidence="4">2.3.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH1988</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1988"/>
    <dbReference type="EnsemblBacteria" id="BAD40973">
        <property type="protein sequence ID" value="BAD40973"/>
        <property type="gene ID" value="STH1988"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1988"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_7_0_9"/>
    <dbReference type="OMA" id="KSWDFHV"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005675">
        <property type="entry name" value="Citramal_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43538">
        <property type="entry name" value="PTHR43538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00977">
        <property type="entry name" value="citramal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="556" mass="59213" checksum="FF989A6D9BFBBEBE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N10</accession>
    <name>AROB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate synthase</fullName>
            <shortName evidence="1">DHQS</shortName>
            <ecNumber evidence="1">4.2.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroB</name>
        <name type="ordered locus">STH1948</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit. Can use either Co(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196075.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N10"/>
    <dbReference type="SMR" id="Q67N10"/>
    <dbReference type="STRING" id="292459.STH1948"/>
    <dbReference type="EnsemblBacteria" id="BAD40933">
        <property type="protein sequence ID" value="BAD40933"/>
        <property type="gene ID" value="STH1948"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1948"/>
    <dbReference type="eggNOG" id="COG0337">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_0_2_9"/>
    <dbReference type="OMA" id="ARAHFIG"/>
    <dbReference type="OrthoDB" id="1677032at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00110">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016037">
        <property type="entry name" value="DHQ_synth_AroB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030963">
        <property type="entry name" value="DHQ_synth_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001455">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01357">
        <property type="entry name" value="aroB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000231136" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00110"/>
        </source>
    </evidence>
    <sequence length="358" mass="38345" checksum="427330F9C1A93942" modified="2004-10-11" version="1">MELGEERYPIYVGAGLMAQLGALVRRHLPETRRALLVTDANVAPLYGEAARAALEQAGITTARVTVPAGEASKSLSQAYSLYSSCVRAELDRTSAVVALGGGVVGDLAGFVAATYLRGIPLVQVPTTLLAQVDSSIGGKTGVDLPQGKNLVGAFHQPSLVVADVETLKSLPRRELSAGMAEVVKHGVIRDEEFLKYVEVQVGNVLAGDPAVLERVVAESCRIKAEVVAADPREKGLRAILNFGHTVGHALEAAIGFRWLHGECVAVGMVAAAHIARHSGIAQEGRLELRLTRLLERLDLPTTLPEGMDPKDLEPFLRRDKKIKSGVIHWVMPVRPGEVVVTPDVSPDAIRWGLEALRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N54</accession>
    <name>Q67N54_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1904"/>
    <dbReference type="EnsemblBacteria" id="BAD40889">
        <property type="protein sequence ID" value="BAD40889"/>
        <property type="gene ID" value="STH1904"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1904"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_969525_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="2">
        <location>
            <begin position="129"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="31062" checksum="54068C95FC526DBB" modified="2004-10-11" version="1">MVPLSRDPNTYNGQPLACIFVASQRDQAVTKTEYGSESFSRPVCSEDSRRVPHQSQRKDLHRPPQISEGGTLQMPRTPEEGRALFDRWARTYDLDLAGQDGPKAGPLVGYAETLRIAAEQVPVAPGMSVLDVGIGTGAFAARFRGRGVRVSGVDPSPAMLDACRGKHPGFTLRVGDFNAIPFDSGEFDMVISSFAFHEVPPGGRLAACRELARVLKPGGALCLVDILFASPAALAAAREAIGERWDDEEDYPLVGDLDQLLHEAGIGGSRWRQTAPCHWMVLARKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N89</accession>
    <name>Q67N89_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1869</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195996.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1869"/>
    <dbReference type="EnsemblBacteria" id="BAD40854">
        <property type="protein sequence ID" value="BAD40854"/>
        <property type="gene ID" value="STH1869"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1869"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318935_0_0_9"/>
    <dbReference type="OMA" id="ERTANLC"/>
    <dbReference type="OrthoDB" id="1887694at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="21704" checksum="D7F9C5C20030635D" modified="2004-10-11" version="1">MKGQSGERTLPFSVLLSSPGPIVVGAGLLIGRSSTQLADFIRRTAELAAIVVSWAVYRVLRRGGEPDSARRRRLERTANGFVGGAMCLSGAAMLVLALFSGGAEKGNVIPGLVIAGLGVTTNGWFWFRYRTLDRREPSAILAAQSRLYRAKTLVDGCVTAALATLALAPASPAARYVDLGGSAAVALYMVISGVLIVRGGSRAERTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-24" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG0</accession>
    <name>KU_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Non-homologous end joining protein Ku</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ku</name>
        <name type="ordered locus">STH1798</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With LigD forms a non-homologous end joining (NHEJ) DNA repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA nor ssDNA. Recruits and stimulates the ligase activity of LigD.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with LigD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic Ku family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NG0"/>
    <dbReference type="STRING" id="292459.STH1798"/>
    <dbReference type="EnsemblBacteria" id="BAD40783">
        <property type="protein sequence ID" value="BAD40783"/>
        <property type="gene ID" value="STH1798"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1798"/>
    <dbReference type="eggNOG" id="COG1273">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048975_2_0_9"/>
    <dbReference type="OMA" id="IRYRKVC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00789">
        <property type="entry name" value="KU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01875">
        <property type="entry name" value="Prokaryotic_Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="Ku70/Ku80_beta-barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009187">
        <property type="entry name" value="Prok_Ku"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41251">
        <property type="entry name" value="PTHR41251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SSF100939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02772">
        <property type="entry name" value="Ku_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000389201" description="Non-homologous end joining protein Ku">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Ku" evidence="1">
        <location>
            <begin position="12"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="402" mass="41840" checksum="FBA85D71B31AA319" modified="2004-10-11" version="1">MDALRAMWKGTISFGLVTIPVKLYAATESKDLRFNLLHEPCQTPVQYRKYCPTCEREVESGEIVKGYEFDRGRYVTLRDEDFESIPLAARRTLEIVAFVRLEEIDPIYFDKTYYLEPGEGGAKAYGLLRHAMEVTGRVAIARVVLRAKESLAALRVFREGVLAMETMHFPDEVRPVAALTGIAQPELRPQEIEMATGLIESLTMPFSPERFENTYREALLELIQAKIAGAPPAEPGPAPEQGRVVDLMEALRASIRAAEAQRAAGGATGGAAGADGTPPAPVARSPEAPARRAIPGLPGDPAASVPGVPAAPVPRAPGVTGAPTPGTPPAAVPGVPATAVPGTPGAPVPTAPGVPSAPAPGTSPTSVPGVQTAPNGAAPPGPFAGSDRAAEVGPDETAPGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ0</accession>
    <name>Q67NQ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1708"/>
    <dbReference type="EnsemblBacteria" id="BAD40693">
        <property type="protein sequence ID" value="BAD40693"/>
        <property type="gene ID" value="STH1708"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1708"/>
    <dbReference type="eggNOG" id="ENOG50324NT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1502740_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="2">
        <location>
            <begin position="121"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="19119" checksum="DF2EF954DF05FBEF" modified="2004-10-11" version="1">MAAAGGLVAAVIWLGTDRDDRLSHTLVRPAVMALLSLLLLVILAEAEFWAYSLSAAAAGIGLWESYLGAYLLMSAPVSRTGPVAQVRTTARWLLNTLANLMLLNLSAQLAFPHTFEWRGIPASALDVAYFTVLTFASGGYGDVLPASPLGKLLAMLTSVSGLLFATILFAALFHRLREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ1</accession>
    <name>Q67NQ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195835.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1707"/>
    <dbReference type="EnsemblBacteria" id="BAD40692">
        <property type="protein sequence ID" value="BAD40692"/>
        <property type="gene ID" value="STH1707"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1707"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_9"/>
    <dbReference type="OMA" id="PYWHQRQ"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="15"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="316" mass="34888" checksum="145468A6B56EF186" modified="2004-10-11" version="1">MEYRNLGRSGLKVSKMALGTNAFGTRTDEATAIRIVHAALDAGINLIDTADMYGQGESERMIGLALKDRRSRAVLATKCWYPTGDGPNDRGASRKHIMDAVDASLRRLQTDYIDLYQIHRWDGDTPIEETMRALEDLVRAGKVRYIGCSNFAAWQIVMANAVADRHGWSRFISNQPEYSPVSRQIEQEVIPACLADGVGQIVYFPLAGGLFTGKYRRGEAPPPDSRAAKQGPRFAERWLTPAHFDLVEAMEHVAREAGLTLTQLTLAWVMARPGVTAAIVGASRPEQVAENVSACEVQLPQEVMDRVTALSEPFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ9</accession>
    <name>Q67NQ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1699</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1699"/>
    <dbReference type="EnsemblBacteria" id="BAD40684">
        <property type="protein sequence ID" value="BAD40684"/>
        <property type="gene ID" value="STH1699"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1699"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_20_9"/>
    <dbReference type="OMA" id="NCYRFFT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="45"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="123"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="174"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="242"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="325"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="4">
        <location>
            <begin position="469"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="720" mass="79716" checksum="C6116813C2BA861A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV8</accession>
    <name>Q67NV8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195779.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1650"/>
    <dbReference type="EnsemblBacteria" id="BAD40635">
        <property type="protein sequence ID" value="BAD40635"/>
        <property type="gene ID" value="STH1650"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1650"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_5_1_9"/>
    <dbReference type="OMA" id="ILAWWRE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="25694" checksum="4EBD23D7BF32D436" modified="2004-10-11" version="1">MEWLIVAIVSLGTSFVKTAFGLGAGVLFGPVLALFLEPRQAMGMTAPVMFLSSVAALAAHWRRWDWPVLRRLLPTALAGLWLGSVFLAQAPAPHLRRAIGVAAVSFAVVQVARLRRGAAVSPAGDWAPGPAVLLGFTGGVVSGIAHSGGLFFSMYLLPRLEKVAFVASLTATLMVVDLFRLVSYWYLDVLQPRHVLLGLLCAPLMLLGGWLGKRLNGRLSSRGFVTALSALIAATGLALLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW3</accession>
    <name>Q67NW3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195774.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1645"/>
    <dbReference type="EnsemblBacteria" id="BAD40630">
        <property type="protein sequence ID" value="BAD40630"/>
        <property type="gene ID" value="STH1645"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1645"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_2_9"/>
    <dbReference type="OMA" id="HETYKGE"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="336" mass="36201" checksum="09632E4DA107A39B" modified="2004-10-11" version="1">MTIRIGINGFGSIGRRVFRIALSRPDIEIVAINDLTPPATSAHLLKYDSNYGILDAEVSATENSIIVNGKEIRVYAEKDPAQIPWKEHGVDIVMECTGRFTDKQQAEVHITSGGAKKVIISAPAKNEDITIVMGVNHEQYDPANHHVISNASCTTNCLAPVVKVLHDKFGVVKGMMNTIHSYTNDQVILDYPHKDLRRARAGALSIIPTTTGAAKAVALVIPELKGKLTGFAMRVPTPVVSVVDFTAELATPTTKEEINAALKAAAEGPMKGILAFEEAPLVSVDFKGNPNSSIVDGPSTLVLDGNLVKVVSWYDNEWGYSNRMVDLAAYIASKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW4</accession>
    <name>Q67NW4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional repressor of xylose-utilizing enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195773.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1644"/>
    <dbReference type="EnsemblBacteria" id="BAD40629">
        <property type="protein sequence ID" value="BAD40629"/>
        <property type="gene ID" value="STH1644"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1644"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_5_9"/>
    <dbReference type="OMA" id="HAPCSRA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="HTH crp-type" evidence="4">
        <location>
            <begin position="14"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="403" mass="43220" checksum="7F4D7C22AF70C016" modified="2004-10-11" version="1">MLVGPELIRAINKQRVLRLVRSAGAMSRADLAERTGLTRPTVSAVVAELLEEGWVEEIGTGESSGGRPPILLRFNPRARWVIGAELGAGHVRAVLADLAGNVFHRFKQRVESRDPLIEVDQLERAVRYLLDQTPRYGPPTPVAGVGIGITGVIDPEEGVWRYSPHYQVRDLPVAPMLQERLSLPVWIENDARAMAWGERSFGAAQGVDNLAFIRVGVGLGAGIIIDGRLYGGAHQGAGEIGHIMVKERGLRCRCGSDGCLETVGSAIAIARRAVQRMAQGEETLIRELCGGDPSKVIATTVIEAADAGDRVAQEILSEAGRFLGIGIGAMINLLNPAMVIIGGGTSRAGDYLIEPLRQAALERTLPALRERVKIVRTELGEDAGPLGGAALVIEELFRTPTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ3</accession>
    <name>Q67NZ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Branched-chain amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195744.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1615"/>
    <dbReference type="EnsemblBacteria" id="BAD40600">
        <property type="protein sequence ID" value="BAD40600"/>
        <property type="gene ID" value="STH1615"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1615"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_3_0_9"/>
    <dbReference type="OMA" id="YIVGCFM"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="31536" checksum="4297E0EE5D11D397" modified="2004-10-11" version="1">MEHLLEQTLNGLQLGFVYALIAVGYTMVYGIIKLINFAHGDVFMVGAFLAYFGFTSWGLPWPVAVLVSMAGCAVLGVGIERLAYRPLRARGAPRIAALITAIGVSLFIEYFTSLNFVFGSDYRNVPRVLPDRRWELGGITVTNVQLLVLVTVLVLVASLQFVVYRTRVGKAMRAVAQDTDAARLMGIDVDRIIACTFALGSALAAAAGALFAVAYPQIHPFMGIMPGLKAFTAAVLGGIGSIPGALLGALVMGQVEVLTAAYVTTDFRDAIAFALLILVLLLRPTGLLGKTAPEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P51</accession>
    <name>Q67P51_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1557"/>
    <dbReference type="EnsemblBacteria" id="BAD40542">
        <property type="protein sequence ID" value="BAD40542"/>
        <property type="gene ID" value="STH1557"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1557"/>
    <dbReference type="HOGENOM" id="CLU_2720845_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="72" mass="8261" checksum="3F38D8F5BDC147DD" modified="2004-10-11" version="1">MNRMPNFREWTPSEIMAQMPEARSVLAKHFGREGISTASGFRLRDLARQKDVELSDVIRDLNDVARATGMPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P98</accession>
    <name>Q67P98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1510"/>
    <dbReference type="EnsemblBacteria" id="BAD40495">
        <property type="protein sequence ID" value="BAD40495"/>
        <property type="gene ID" value="STH1510"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1510"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_6_1_9"/>
    <dbReference type="OMA" id="KSYAIFY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="35"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="26477" checksum="1225012041C41AE2" modified="2004-10-11" version="1">MYGIPYPRRARTQPFSGREPALLQSEVFGVMKVAAVIPAYNEESTIGAVVSACRQVPEISEVIVVSDGSTDETATLARAAGATVIEHTENRGKAAAMKSGLEATTAPVLLFLDADLIGLSADHLRLLLRPVLAGEADMSIGVFGDGRVATDLAQMLAPYLSGQRAVRRELLADMFREEPEAEQVRFGIEVALTKYVKQHGCRVVEVVLEEMSHRMKEEKLGLVKGAAARLKMYYEILKYVQKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PA7</accession>
    <name>ISPG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)</fullName>
            <ecNumber evidence="1">1.17.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispG</name>
        <name type="ordered locus">STH1501</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + H2O + oxidized [flavodoxin] = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:43604"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:10623"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspG family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195631.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PA7"/>
    <dbReference type="SMR" id="Q67PA7"/>
    <dbReference type="STRING" id="292459.STH1501"/>
    <dbReference type="PRIDE" id="Q67PA7"/>
    <dbReference type="EnsemblBacteria" id="BAD40486">
        <property type="protein sequence ID" value="BAD40486"/>
        <property type="gene ID" value="STH1501"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1501"/>
    <dbReference type="eggNOG" id="COG0821">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042258_0_0_9"/>
    <dbReference type="OMA" id="PTCGRTQ"/>
    <dbReference type="OrthoDB" id="665306at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00096"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046429">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00159">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016425">
        <property type="entry name" value="IspG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004588">
        <property type="entry name" value="IspG_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30454">
        <property type="entry name" value="PTHR30454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04551">
        <property type="entry name" value="GcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004640">
        <property type="entry name" value="IspG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00612">
        <property type="entry name" value="ispG_gcpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000190637" description="4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00159"/>
        </source>
    </evidence>
    <sequence length="370" mass="40241" checksum="66129BEB1DF9F7DB" modified="2004-10-11" version="1">MIERRKTRAVKVGRVQIGGGAPITVQSMTKCDTRDVPEVLRQIRALEEAGCDIVRVAAPTMEAAECFKEIRKGCNIPLVADVHFDYRIALKVLEAGIDKLRINPGNIGARWKVEEVVRACKDRGVPIRIGVNAGSLEEEFLEKYGYPTADGMVESALKHVAILEELDFHDIVISIKSSRVDQMIEAYRKLSEKVDYPLHVGVTEAGTPFAGTIKSAVGIGTILAEGIGDTIRVSLSTDCVEEVRVGYEILKALGLRTRGINIISCPSCGRVQIDLVKVANEVERRLQHIDVPLNVAVMGCVVNGPGEAAEADVALFGGKGVGMLYVGGERVRKTTEEEMVEALVELVEQKAAEIKASGRVPTGYGRHEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK1</accession>
    <name>Q67PK1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="5 11">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpE</name>
        <name evidence="14" type="ordered locus">STH1407</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="5 11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195537.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1407"/>
    <dbReference type="EnsemblBacteria" id="BAD40392">
        <property type="protein sequence ID" value="BAD40392"/>
        <property type="gene ID" value="STH1407"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1407"/>
    <dbReference type="eggNOG" id="COG0147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_9_3_9"/>
    <dbReference type="OMA" id="GCVGYLD"/>
    <dbReference type="OrthoDB" id="1786019at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005256">
        <property type="entry name" value="Anth_synth_I_PabB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00564">
        <property type="entry name" value="trpE_most"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anth_synt_I_N" evidence="13">
        <location>
            <begin position="28"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="12">
        <location>
            <begin position="238"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD40392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="505" mass="56097" checksum="8032E03B84AABDF2" modified="2004-10-11" version="1">MYHPSLGTYLSLAREYDLVPVHREVLADLETPISVFMKLCLDEPCAFLFESVEGGEKVARYSFLGCRPLLTLAHKAGVSRLLRGEEAAEYRTVGMVTEGERGERMEGDPLGHLRQVLRRYAVYQPPGLPRFAGGLVGYVAYDAVRAYERLPPGPPDELNLPDSLFMAPELVVVFDHLKHKLLLVANTMPGGEPDVAYRRAQALLDRAVERLRGPVPLPPGLPWTRSRPLEVRACQTPEDFTAAVERAKAYIRAGDAFQVVLSQRWETRVRSRPLDIYRALRMLNPSPYMFYLSFGDLKVIGASPELLVRVEDGVATTRPLAGTRPRGATEEEDRRLEAELLADEKERAEHVMLVDLGRNDLGRVCNYGTVHVHQLMHVERYSHVMHIVSDIRGEVAEEKDACDVLAACFPAGTLTGAPKVRAMEIIDELEPVRRGIYGGAVGYFSWSGAMDTCIAIRTLIMKGDRVFIQAGAGIVADSDAEAELNESRNKARALIRALELAEEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK2</accession>
    <name>Q67PK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multidrug efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1406</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1406"/>
    <dbReference type="EnsemblBacteria" id="BAD40391">
        <property type="protein sequence ID" value="BAD40391"/>
        <property type="gene ID" value="STH1406"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1406"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_60_4_9"/>
    <dbReference type="OMA" id="WESNLSV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="394" mass="41831" checksum="AA138774CAEF260B" modified="2004-10-11" version="1">MLWFLAIGSFLNSTGMSFIWPLTTIYIHEHLGRSMTVAGTVLLLHSAGAMLGQLAGGWCHDRIGPRPVMLGGLLAAAAVTAVLGWTTAWPVYVACMVLYGIAVALPLSPVNAMVARSWPGQGRRAFNFNYVASNLGVAAGTALGGVLADRSFALAFGGASCLFLVYTLFTAAFIREERLPAEPFDEGAYPRREAVPEGPVPWAPIAALCAGFLCVTLVYSQWQTGNSIRTQELGFGLSAFSLLWTLNGALIFVGQPVIGLIARVLQRAASQMMLGVALLALAFGVLLTSDRYGIFVLSMVLLTFGEMLLWPVFPASVAHLSPPSMRGRLQGFILSSGTIGRMIGPLLGGMLYDAFGYATQISVMTAGLVVPALAILVYERTRRVAEAGVEPGDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK9</accession>
    <name>Q67PK9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1399"/>
    <dbReference type="EnsemblBacteria" id="BAD40384">
        <property type="protein sequence ID" value="BAD40384"/>
        <property type="gene ID" value="STH1399"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1399"/>
    <dbReference type="eggNOG" id="COG0071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1642871_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="44"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="16868" checksum="C56E233F455DF62C" modified="2004-10-11" version="1">MQRIAEQLEALGDAFGEELFRELGRAGTPRPKGQSGGRIGGRARTGAAAGPPLDIYVTPSEVVVEAILPGLGGPHQVTVSLAGPAELLIEAFLPDRLPDGLYIQRERFVGYCVRMVTLPATVLPAASARYRHGILQLRFPRATPGSGGEGVAVLHVPPTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP3</accession>
    <name>Q67PP3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative surface adhesion</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1365"/>
    <dbReference type="EnsemblBacteria" id="BAD40350">
        <property type="protein sequence ID" value="BAD40350"/>
        <property type="gene ID" value="STH1365"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1365"/>
    <dbReference type="eggNOG" id="COG3936">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064947_0_0_9"/>
    <dbReference type="OMA" id="YHFYFVP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="2">
        <location>
            <begin position="9"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="40297" checksum="1DD0E8BC225E2C27" modified="2004-10-11" version="1">MKQKVIVPELIIIRAVSCLAVVLMHSITMLIGDPEVNRGLNTARLLLLFATPTFAYMSAFLIGYSKSQRPYFETLWLRVKYLILPYLIFAVGYACWQHYQWGAPLVDRIRYNIAGGYHGYFVLVVFQFYLFHPLFRPILERVPAWVGVPAAGLVNIGYLAALNLGLVSIPYLGFHAYRLLPAWFFYYVLGYYSGRDRDQFQLVLRRWWPLSAALLACGAGLLLRNALSGFLPDVTSQRFEVVLYAAGVILLAFPLAGRARRIPSVLNLVNRASFGIYLLHWFFLELFRRWVVRIPGLPKPAGILLLFVLAATAAALVTFALNRWRWGAYVVGRLGVDGSKARLAPPPGSAAAPAEAATR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR5</accession>
    <name>DEF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide deformylase</fullName>
            <shortName evidence="1">PDF</shortName>
            <ecNumber evidence="1">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">def</name>
        <name type="ordered locus">STH1343</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195474.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PR5"/>
    <dbReference type="SMR" id="Q67PR5"/>
    <dbReference type="STRING" id="292459.STH1343"/>
    <dbReference type="EnsemblBacteria" id="BAD40328">
        <property type="protein sequence ID" value="BAD40328"/>
        <property type="gene ID" value="STH1343"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1343"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_4_2_9"/>
    <dbReference type="OMA" id="NMHGEPV"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000301114" description="Peptide deformylase">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="217" mass="23242" checksum="DAA0BEF5C915026C" modified="2004-10-11" version="1">MAILEIVKEPAEVLRKKAKPVTKINASIRKLLDDMTETMYAAPGVGLAAPQVGVSKRLIVVDPQDGSGQLYQLINPEIVKAEGWVKGTEGCLSIPGMVGDVWRYEKVQVVALDRTGKKVWIDAEGYLARIFQHEIDHLDGILYTDKCTNLRPVSEDGEEEEEAEVAEVMPEPEAEGAGEPSAEGAGQAAAEAGAEEGEQVTTGVSGERRGSAAAKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PX2</accession>
    <name>Q67PX2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron-siderophore uptake ABC system substrate-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1286"/>
    <dbReference type="EnsemblBacteria" id="BAD40271">
        <property type="protein sequence ID" value="BAD40271"/>
        <property type="gene ID" value="STH1286"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1286"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062437_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269932" evidence="1">
        <location>
            <begin position="39"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="70"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="347" mass="37336" checksum="9702798492F1AFE0" modified="2004-10-11" version="1">MLIRKLIPIRNSRLFPVLALLLAALLLAGCTNASGGGAAPGGSGSSPEPAESWSFVDDRGITVTLPERPTRVVAQSTAAAALWSLGVEVVGVFGPQRLPDGSNDPTVGDVDLSRVTSLGEAWGELDLEKLAALKPDLIVSIMYTPPELWYVLPDVEPQVRAIAPTVGITVGQRSIKEAMQRFEELAGALGADLNAPQVAAARQRFEEASEELRAALAEKPGLTAMFGAGSLETLWVSNPPYYGDLSYFQELGMQILVPENPESYWEALSWEQALRYQADVLFYDSRTEVTQPPELAAQVPTWSHIPAVKAGQVYPWVAVPPYSWDGLATILEDVAAAVREADPHVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q21</accession>
    <name>Q67Q21_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydro_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1237"/>
    <dbReference type="EnsemblBacteria" id="BAD40222">
        <property type="protein sequence ID" value="BAD40222"/>
        <property type="gene ID" value="STH1237"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1237"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_1_0_9"/>
    <dbReference type="OMA" id="VAWVGSE"/>
    <dbReference type="OrthoDB" id="751534at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="2">
        <location>
            <begin position="51"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="536" mass="57749" checksum="A4F85FBE2AD46480" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q32</accession>
    <name>Q67Q32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1226"/>
    <dbReference type="EnsemblBacteria" id="BAD40211">
        <property type="protein sequence ID" value="BAD40211"/>
        <property type="gene ID" value="STH1226"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1226"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073023_1_0_9"/>
    <dbReference type="OMA" id="QVICVEN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014193">
        <property type="entry name" value="CHP02828_mem_fusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032681">
        <property type="entry name" value="HlyD_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12700">
        <property type="entry name" value="HlyD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02828">
        <property type="entry name" value="TIGR02828"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="300" mass="31802" checksum="E10BC434E5E853FA" modified="2004-10-11" version="1">MILLAVAALGTGLYIWNPAVLLTAAPAEPDPLAGVRQGSLRVTLEADAVLVRREQVYRAPQAGTLRRLAAEGARVRVGGQVVAFAPGQSAAALEEVITTPVSGVVFYQTDGLEEVLTPEAVAGWGPEAFSTLPYPDTREIADGPVEAGEPLFKLVDDLSLEMLLRVSADSLSESQQAVLPGQEVELQVSGRDLPLTARVVRLAAAGPDLLVHLEAPLPSADALRLRRMRVSLLLAEYEGIILPRAAIDVEQGRTGVWIAQRGGYRFVPVRVLGGTADEVAVEGDVPPDAQVLTAPPPRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q51</accession>
    <name>Q67Q51_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="7">6.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">D-alanyl-D-alanine-adding enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murF</name>
        <name evidence="12" type="ordered locus">STH1207</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MurCDEF family. MurF subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1207"/>
    <dbReference type="EnsemblBacteria" id="BAD40192">
        <property type="protein sequence ID" value="BAD40192"/>
        <property type="gene ID" value="STH1207"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1207"/>
    <dbReference type="eggNOG" id="COG0770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031507_1_1_9"/>
    <dbReference type="OMA" id="TIINDAW"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="9">
        <location>
            <begin position="27"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="110"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="10">
        <location>
            <begin position="347"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="521" mass="53261" checksum="49F7A074DE49EE9E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q58</accession>
    <name>MRAZ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>Transcriptional regulator MraZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mraZ</name>
        <name type="ordered locus">STH1200</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Forms oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MraZ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q58"/>
    <dbReference type="SMR" id="Q67Q58"/>
    <dbReference type="STRING" id="292459.STH1200"/>
    <dbReference type="EnsemblBacteria" id="BAD40185">
        <property type="protein sequence ID" value="BAD40185"/>
        <property type="gene ID" value="STH1200"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1200"/>
    <dbReference type="eggNOG" id="COG2001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107907_0_5_9"/>
    <dbReference type="OMA" id="RGQERCL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16321">
        <property type="entry name" value="MraZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16320">
        <property type="entry name" value="MraZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01008">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003444">
        <property type="entry name" value="MraZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035644">
        <property type="entry name" value="MraZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020603">
        <property type="entry name" value="MraZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035642">
        <property type="entry name" value="MraZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038619">
        <property type="entry name" value="MraZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34701">
        <property type="entry name" value="PTHR34701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02381">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00242">
        <property type="entry name" value="TIGR00242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000108547" description="Transcriptional regulator MraZ">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB 1" evidence="2">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB 2" evidence="2">
        <location>
            <begin position="74"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <sequence length="138" mass="15386" checksum="49860FD0103DB692" modified="2004-10-11" version="1">MGEFQHAIDAKGRLIIPAKLREGLGERFIATKGLDRCLFVFPLAEFEAVSQKLRGLGMSSSAARAFNRLFFSGATECELDPQGRILLPANLREYAGIQKDCVIVGVENRVEIWAAERWAEYSEEAGELYTEIAEKLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q67</accession>
    <name>Q67Q67_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Biotin carboxylase</fullName>
            <ecNumber evidence="5">6.3.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetyl-coenzyme A carboxylase biotin carboxylase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] = ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195322.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1191"/>
    <dbReference type="EnsemblBacteria" id="BAD40176">
        <property type="protein sequence ID" value="BAD40176"/>
        <property type="gene ID" value="STH1191"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1191"/>
    <dbReference type="eggNOG" id="COG0439">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_9"/>
    <dbReference type="OMA" id="FVEICSH"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="7">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="120"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="449" mass="49265" checksum="236A4E2E68D24165" modified="2004-10-11" version="1">MFKKVLIANRGEIAIRIIRACRELGIKTVAVFSEADREALHVRMADEAHYIGPAPSNQSYLSVPAIITAATASGADAIHPGYGFLAENPFFAEACRQWNIAFIGPHPEAIRAMGMKDASKALMKKAGVPTIPGSDGVVESEAEALRVAEEIGYPVMVKATAGGGGRGIRIANSPDELVQALQKAASEAGAAFGNAAVFVEKYIQVGRHIEVQVLGDQHGNIIHLGERECSIQRRRQKMLEEAPAPFLPEETRQRIREAGVRAARAVGYDNAGTVEMIYDEMTGEFYFLEMNTRIQVEHPVTEAVTGIDLVKAQIRVAAGEPLPWKQEDIVIRGHAIECRVNAEDPDNKLYPSTGTVTALEIPGGPWTRWDGMLYPGYQMKPFYDSLVGKIIVWGEDRSDAIAKMQRALAEFRVEGIKTTVPVHQRILASSEFREGRYSVEWLEGFLGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q73</accession>
    <name>Q67Q73_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1185"/>
    <dbReference type="EnsemblBacteria" id="BAD40170">
        <property type="protein sequence ID" value="BAD40170"/>
        <property type="gene ID" value="STH1185"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1185"/>
    <dbReference type="HOGENOM" id="CLU_1577700_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="18383" checksum="9E11F8272ACFB11D" modified="2004-10-11" version="1">MALSSIHAAQAGSSIRPTWSNIAFNSAAVAGSIRLARSAPPSFTRREAAAQRAENWPSLAATWGMDSTWYRSQTARDIGTSDLSRALLTMRPLISDATFPGLLPYTRTRWAKGSIRADQVSADPPEPWFQRVGRTQATCRELRPGATRRSRSTGSPARWCCAACSQSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q83</accession>
    <name>Q67Q83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195306.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1175"/>
    <dbReference type="EnsemblBacteria" id="BAD40160">
        <property type="protein sequence ID" value="BAD40160"/>
        <property type="gene ID" value="STH1175"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1175"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_1_4_9"/>
    <dbReference type="OrthoDB" id="1992113at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="117"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20794" checksum="5368CB01649FDD4F" modified="2004-10-11" version="1">MREGVEMWAQPALSDDEQLGRLMEVMGGSLLSYANRLLGDPFAAEEVCQDAMYKAWCHMESLEPTSQLRAWLYKVTRNAALDVLRSQRHQTTALLTEDLLDPTPGPESELERKAFVEALREALHRLPQHYNSVLSLRLLAGYSYRDIAEELNLPLGTVKSRLRNGLQYLSRELASVGVDQGYLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q98</accession>
    <name>RUVA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvA</name>
        <name type="ordered locus">STH1160</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195291.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q98"/>
    <dbReference type="SMR" id="Q67Q98"/>
    <dbReference type="STRING" id="292459.STH1160"/>
    <dbReference type="EnsemblBacteria" id="BAD40145">
        <property type="protein sequence ID" value="BAD40145"/>
        <property type="gene ID" value="STH1160"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1160"/>
    <dbReference type="eggNOG" id="COG0632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087936_3_0_9"/>
    <dbReference type="OMA" id="VGMAVQC"/>
    <dbReference type="OrthoDB" id="1370858at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14332">
        <property type="entry name" value="UBA_RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00084">
        <property type="entry name" value="ruvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000224916" description="Holliday junction ATP-dependent DNA helicase RuvA">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <sequence length="205" mass="21467" checksum="50C8FAA2A899CB01" modified="2004-10-11" version="1">MIAHLRGELVTAGADWVVIDVAGVGYRCLVPASTRSRLPAQGAAVQLYTYLQVREDALTLYGFLTQAEYDLFELLLRVDGVGPKVALAVLSTTDPAAFRRAVAFEDLDAICRVPGIGRKTAQRLVLELKDKIGAVPAGGGGVPDGLPVAVAPAGDAWAEASEALIALGYSRGEAAAALARVRAEAGEAPSVETLVRLALKQLYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB6</accession>
    <name>Q67QB6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Adenosine 5'-phosphosulfate reductase</fullName>
            <shortName evidence="10">APS reductase</shortName>
            <ecNumber evidence="6 10">1.8.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">5'-adenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Thioredoxin-dependent 5'-adenylylsulfate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cysH</name>
        <name evidence="12" type="ordered locus">STH1142</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the formation of sulfite from adenosine 5'-phosphosulfate (APS) using thioredoxin as an electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 10">
            <text>[thioredoxin]-disulfide + AMP + 2 H(+) + sulfite = [thioredoxin]-dithiol + adenosine 5'-phosphosulfate</text>
            <dbReference type="Rhea" id="RHEA:21976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="1.8.4.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 10">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the PAPS reductase family. CysH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.10" evidence="6 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195273.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1142"/>
    <dbReference type="EnsemblBacteria" id="BAD40127">
        <property type="protein sequence ID" value="BAD40127"/>
        <property type="gene ID" value="STH1142"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1142"/>
    <dbReference type="eggNOG" id="COG0175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044089_2_0_9"/>
    <dbReference type="OMA" id="PIARWTQ"/>
    <dbReference type="OrthoDB" id="674332at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043866">
        <property type="term" value="F:adenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011798">
        <property type="entry name" value="APS_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000857">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02055">
        <property type="entry name" value="APS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00434">
        <property type="entry name" value="cysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="11">
        <location>
            <begin position="39"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; cysteine thiosulfonate intermediate" evidence="10">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="26421" checksum="512407A8DF3DC50D" modified="2004-10-11" version="1">MAEKRWSPEEIAAAARSLEGQGPVAALRWAYEQFAPEEIAFACSFGAEDVALAHMISRVAPGAQVFYLDTDFLFPETYRTIEAVRAAYPLRIRRVSPALTPREQAERYGDELWSRDPDLCCSLRKVEPLREVLRGLKAWITGIRRAQSPTRAGAQVVEWDRKFGLVKVNPLVDWKDADVWKYILEHNVPYNPLHDRGYPSIGCTHCTRPVRPGEDPRAGRWSGFAKTECGLHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC3</accession>
    <name>Q67QC3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Secreted peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1135"/>
    <dbReference type="EnsemblBacteria" id="BAD40120">
        <property type="protein sequence ID" value="BAD40120"/>
        <property type="gene ID" value="STH1135"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1135"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1255415_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269494" evidence="1">
        <location>
            <begin position="28"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="2">
        <location>
            <begin position="76"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="220" mass="24531" checksum="D9046D15C195AE88" modified="2004-10-11" version="1">MVRMVKLIAALALIVGIGFHANNFVEAASWYFFVHPTSETSPNYYYDLDRRVNYMRDWTGWSCSNCTDVWGHTYDQHMGTDYPLANGRPIRSAQQGTVTEVVNYQYNQCGVNTGPVFGTYVKIRHPAHGSSGKVYYTTYAHMTYQSPAVTVGQNVGIYQYLGAVGQTGNACGYHLHWAVEETNLGNYVDPFDKGLVCGFYGSAPCSVEPSQLDEDVELDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QD0</accession>
    <name>Q67QD0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1128"/>
    <dbReference type="EnsemblBacteria" id="BAD40113">
        <property type="protein sequence ID" value="BAD40113"/>
        <property type="gene ID" value="STH1128"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1128"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037521_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025668">
        <property type="entry name" value="Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13701">
        <property type="entry name" value="DDE_Tnp_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tnp_DDE_dom" evidence="2">
        <location>
            <begin position="296"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="530" mass="59920" checksum="035B79A1404222E9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QJ3</accession>
    <name>Q67QJ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195197.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1065"/>
    <dbReference type="EnsemblBacteria" id="BAD40050">
        <property type="protein sequence ID" value="BAD40050"/>
        <property type="gene ID" value="STH1065"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1065"/>
    <dbReference type="HOGENOM" id="CLU_1049407_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269622" evidence="2">
        <location>
            <begin position="30"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="27013" checksum="E86698350183C3E2" modified="2004-10-11" version="1">MTPHRFGRAALLLAGLVLAGCGRSGPSEAPNPNGIDPAAPVAAGHAALPLPGEPTPLTGRALSGLYASPPSADTLEALSTAPVSPDGLFRAVLHGDELWVTRIDGAWLWQVTPPPVGTPSPADAGSGAQPGAGGPDASPDSGSPPGAGIATAAQAGADGADVRPLAPIQWTPRSTLLFRDTAQRWLEADPETALVTPLSAFLTGATSLIPSPDGSQVLFYKENRLYTARWDGSQPQLVGESVVGHWDSAGRLVVTERPADTVNPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP7</accession>
    <name>Q67QP7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195143.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1011"/>
    <dbReference type="EnsemblBacteria" id="BAD39996">
        <property type="protein sequence ID" value="BAD39996"/>
        <property type="gene ID" value="STH1011"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1011"/>
    <dbReference type="eggNOG" id="COG0170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042633_0_0_9"/>
    <dbReference type="OMA" id="HIALGGW"/>
    <dbReference type="OrthoDB" id="1421771at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="PTHR13353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="482" mass="49269" checksum="E8DC3803B4BEBDA6" modified="2004-10-11" version="1">MNDLWAILLAFGYVGGVVAVGEVGYRMGLPRPIARKVVHVGAGLWIIGTLLVFENRYLAAVPPALSAAANWVVHRRRLLGAVEADADNLGTVWFAVSFALLVLLAWEEPAAMLGGVLAMTLGDAAASTVGLRLGRRPYATLGGRTKSLEGSLAMFAVTLSTLLVVLRLFAPDLAAPALAGLALLGAVAASCAEALGIKGLDNLWVPLGTGFVVWSGLNLVTPLLPALGLGSLLASLIGLAAWRKGALTPSGVLGAILTGTAVFGLGGWPGGLALVAFFVSGSLLSRLFRQRKAVVEADFAKTGTRDLGQALANGVVAAVAAVAYTATGQAAWMGAILGSLAAAAADTWATELGVLARSAPRLITTFKPVPPGTSGAVSVGGTLAAVGGGLFIALVGALADPRWWRLLPWVTLAGLAGSFLDSLLGATLQGVYWCPQCGKETERPVHGCGSRTRLHRGLQVVSNDLVNLLATVVGGLVGFLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS2</accession>
    <name>Q67QS2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH986</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH986"/>
    <dbReference type="EnsemblBacteria" id="BAD39971">
        <property type="protein sequence ID" value="BAD39971"/>
        <property type="gene ID" value="STH986"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH986"/>
    <dbReference type="eggNOG" id="COG3275">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020473_3_0_9"/>
    <dbReference type="OMA" id="RVARNEM"/>
    <dbReference type="OrthoDB" id="1031920at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010559">
        <property type="entry name" value="Sig_transdc_His_kin_internal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06580">
        <property type="entry name" value="His_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="371"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="477" mass="52492" checksum="3364206921C98255" modified="2004-10-11" version="1">MDPGRIESRQGCVLFDDTPRRSDIARPGAAGGSGGFAVGGVPVVNRRARALLLAMVGVGGAALVWLVWARPPRAEMWAAAVVVLQLVLAAWLLTRQDDEESISPAVLESITSFDTSLRIGEETMPYLKQGLNFESAQKICEIIRQITEMDAVAITDDKVILGFSGVGCRRHRQGGPILTGATRHVLETGEARVVNDPRVLSCDEPGCPHPLKAAVITPLKFRGKVVGTFKLYRASNAPVPTYVVRLAAGIAALLGIQMELAESERQRQLVVRARLEALQAQIRPHFLFNVLNTIIMFSRTDPERSRELLVSLAQFFRRSLTTRGNMNTFQDELEYIHIYLSLEQARFGEKLRVKMKVDPRVTGAMVPVLTLQPLVENAVVHGLAPKEDTGVVGIRARQVGSHLHILIADNGVGMDRETQRRVFEEGYGNNMGLGLTNVNERLISLYGPQYRLRIRSTPGRGTAIRLRIPLQHAAQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ5</accession>
    <name>Q67QZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH913</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195045.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q67QZ5"/>
    <dbReference type="STRING" id="292459.STH913"/>
    <dbReference type="EnsemblBacteria" id="BAD39898">
        <property type="protein sequence ID" value="BAD39898"/>
        <property type="gene ID" value="STH913"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH913"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007487_1_1_9"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="4">
        <location>
            <begin position="26"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="5">
        <location>
            <begin position="173"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16" evidence="4">
        <location>
            <begin position="515"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="5">
        <location>
            <begin position="665"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="921" mass="99803" checksum="B4ECCDB485085CBA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R07</accession>
    <name>Q67R07_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ion-translocating oxidoreductase complex subunit B</fullName>
            <ecNumber evidence="5">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Rnf electron transport complex subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnfB</name>
        <name evidence="8" type="ordered locus">STH901</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 3 [4Fe-4S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195033.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH901"/>
    <dbReference type="EnsemblBacteria" id="BAD39886">
        <property type="protein sequence ID" value="BAD39886"/>
        <property type="gene ID" value="STH901"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH901"/>
    <dbReference type="eggNOG" id="COG2878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053470_0_0_9"/>
    <dbReference type="OMA" id="MLPGANC"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00463">
        <property type="entry name" value="RsxB_RnfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010207">
        <property type="entry name" value="Elect_transpt_cplx_RnfB/RsxB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S" evidence="7">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="203"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Hydrophobic" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="271" mass="27000" checksum="E262D59DA59DE900" modified="2004-10-11" version="1">MMTAALVLGGAGLVLGAGLAFVAVKIAVPPDERVVAVREVLPGANCGACGFPGCDGLAAALVRGEAKPDACAAGGPATAAAVAGVLGVEAAVGERLVVHVHCVGSDAKARRTYLYQGIADCRAAALMPGGGPKACSYGCVGLGTCVRACPFDALHMDEAAGLPVVDRQKCTGCGICTQECPKGIMALVPASQATVVSCRNVDKGPQVRKVCTAGCIACGLCVRQCPQKTIAMVNNVAYIDPSGCDGCGICAEKCPTKCIVATQPVPAVEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R51</accession>
    <name>Q67R51_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH857</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH857"/>
    <dbReference type="EnsemblBacteria" id="BAD39842">
        <property type="protein sequence ID" value="BAD39842"/>
        <property type="gene ID" value="STH857"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH857"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_789685_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="36102" checksum="38ACC2CE3313ED6A" modified="2004-10-11" version="1">MKGVWRKSVRRGEGPTPGESAWHVANRASGGAAARQAGPTPSPPHRRHGDRPPGLMDLIWAGLRRSPGQTAAGLLAGLLAALALVGGLSLFQGMGRMLEQGLDRLGADMVLARPEHRPLVEQWLATGATEPIPATIDVARWRQGVDEAQILGLAGVEAVDLSAGGPGVPAGELASILVLRLQFWESAMMARAALADVLPEADAVVGEQATRHVLTDLQPLVRHLGRAAAVAALGASLVAGLLASVRVGQRRAELGMLRAMGATRGYLVALTVGESCALALAGALAGGLLVVGALWLIPATADVLRFLGVLPALGLVGLAAAAITVLSALASLPTALQAAWLDPLEAARRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R94</accession>
    <name>Q67R94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methylthioadenosine phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH814"/>
    <dbReference type="EnsemblBacteria" id="BAD39799">
        <property type="protein sequence ID" value="BAD39799"/>
        <property type="gene ID" value="STH814"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH814"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1106666_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="2">
        <location>
            <begin position="63"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="26567" checksum="FFD3D9A45B63A2BA" modified="2004-10-11" version="1">MAAGVGSPGAGHPGLRHPGTGAPETAYSGTGDFDRLILHETPYGKVEQRGPVLMRTAEADPRALIYAAKAAGASAVLAAARVEPVSPLLEPGDLVVPVDVIDLTRLLPPTFFVGKGYGFIRLDPPFCPELSAALYAAGREKTPRAFRGATYVGLDGSREATPAERRMYRAWGADVVGSGLVPEAFLARELELCYAALTVVREAQADVLAVLERAAQLAPVRRSCSCGRAMAGARAQGLIGEDWREWVRGNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH7</accession>
    <name>Q67RH7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194864.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH731"/>
    <dbReference type="EnsemblBacteria" id="BAD39716">
        <property type="protein sequence ID" value="BAD39716"/>
        <property type="gene ID" value="STH731"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH731"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="IVGFKMG"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="25772" checksum="3785200A8C39BB1C" modified="2004-10-11" version="1">MSVARRILVADDDPKTLKVLEHALTAEGFAVIKASDGQEALDLALSQRPDLVILDVMMPKVDGFAVCGRIRAASAVPILLLTARGDSVDKVVGFRLGADDYVTKPFDLNELVLRVHAILRRMPTLPESRTLRFGHLEINKSTRTVHVGDRNPELTPREFDLLWLMASHPGHPFTRESLLARVWHGDAPTDQSTVTVCIRRLREKIEDNPAQPRWIKTVWGIGYKFDPAGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RN1</accession>
    <name>Q67RN1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194810.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH677"/>
    <dbReference type="EnsemblBacteria" id="BAD39662">
        <property type="protein sequence ID" value="BAD39662"/>
        <property type="gene ID" value="STH677"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH677"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="ITLRIMP"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="28462" checksum="09A238DC36FBA07F" modified="2004-10-11" version="1">MVPVLEARALTKEYARGRLPVTVLRGVDVQVHPSEFLAIMGPSGSGKSTLLHILGALDQPTGGEIRFEGQLVPEWGREPGATEFRRRRLGFVFQSFNLVRSLTARENVGLPLILQGAPASVVERETEVWLKRVGLWDRRDHYPYELSGGEQQRTAVARALVHNPALLLADEPTGNLDSANGLQVLDLLERVCREQGTTVLLVTHDPVAASRADRVVYLRDGRIVDEWEQGRDWTRSMRVQHTLSRLQHLVEGEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RP7</accession>
    <name>Q67RP7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194794.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH661"/>
    <dbReference type="EnsemblBacteria" id="BAD39646">
        <property type="protein sequence ID" value="BAD39646"/>
        <property type="gene ID" value="STH661"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH661"/>
    <dbReference type="HOGENOM" id="CLU_1524374_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="20683" checksum="1C3CB9E4D895FABF" modified="2004-10-11" version="1">MEPLWNLIRTLFATHRPRRVGGIDPRHVGRYAKADLLAELEGYVRRLDGALLETERYPRFRGLLQEMLDQESEQKVSALDRQLFFDFLWDLIRLAEADESPDASFWRQALWNLDGAARHAWALEVRDRTMTRNVLWSARERYLGARVIVRTHNYHAVKDPEAIARAAPEYGRQVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU1</accession>
    <name>Q67RU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194750.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39602">
        <property type="protein sequence ID" value="BAD39602"/>
        <property type="gene ID" value="STH617"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH617"/>
    <dbReference type="HOGENOM" id="CLU_2792490_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="68" mass="7263" checksum="8A8AEAEA87306446" modified="2004-10-11" version="1">MEAEPSCPLPAVQGGCGRDASETILLEVVGFEGEDDTVRISGSEVHVRLSAELRNLVQQRLNEAAVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW8</accession>
    <name>Q67RW8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isocitrate lyase</fullName>
            <ecNumber evidence="1">4.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Isocitrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Isocitratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Can also use Mn(2+) ion.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194724.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH590"/>
    <dbReference type="EnsemblBacteria" id="BAD39575">
        <property type="protein sequence ID" value="BAD39575"/>
        <property type="gene ID" value="STH590"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH590"/>
    <dbReference type="eggNOG" id="COG2224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_0_9"/>
    <dbReference type="OMA" id="LEKDWAE"/>
    <dbReference type="OrthoDB" id="152545at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="PTHR21631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="190"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="311"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="47761" checksum="988770987BD087B5" modified="2004-10-11" version="1">MRDKVRQQAAELEREWRTNPRWAGVERPYSAEDVVRLRGSFQVEYTLARRAAEKLWHLLHTEPYVPALGALTGNQAVQMVKAGLKAIYLSGWQVAADANLAGQMYPDQSLYPANSVPAVVQRINNALRRADQIAWSEGRDDIDWMVPIVADAEAGFGGPLNAFELMKAMIEAGAAAVHFEDQLASEKKCGHMGGKVLVPTSQHIRTLIAARLAADVMGVPTLIVARTDSLGANLLTSDIDPRDREFITGERTPEGFYKVRNGIEAAIARGLAYAPYADLIWCETATPDLDEARQFAEAILSRYPNKLLAYNCSPSFNWKRHLDDDTIARFQRELGAMGYKFQFITLAGFHSLNASMFELARGYAREGMTAYVRLQEKEFALEREGYTATRHQREVGTGYFDEVAQVISGGASSTLAMRGSTEEQQFNVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S40</accession>
    <name>Q67S40_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative molybdopterin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH518</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH518"/>
    <dbReference type="EnsemblBacteria" id="BAD39503">
        <property type="protein sequence ID" value="BAD39503"/>
        <property type="gene ID" value="STH518"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH518"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_9"/>
    <dbReference type="OMA" id="CPLDCPD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02786">
        <property type="entry name" value="MopB_CT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037920">
        <property type="entry name" value="YoaE_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="713" mass="79057" checksum="D1D97569636C9E5B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S49</accession>
    <name>Q67S49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH509"/>
    <dbReference type="EnsemblBacteria" id="BAD39494">
        <property type="protein sequence ID" value="BAD39494"/>
        <property type="gene ID" value="STH509"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH509"/>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049311_3_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="250" mass="28073" checksum="90DD057B8CD2E1CE" modified="2004-10-11" version="1">MKRRLFSGLYRFGSLRWVEWMAACLVAPDHPELARAQGELLVSTPRRVLAFTAHPDDLEFFCGGTLRRMALAGSRITAVVLTDGEKRGNWRDLAEQRQAEQWQAARLQGYEAVHFLGLPDFGLAEDPRLERLLARAWDHAGPEVVLAFDPKELVPQMAHRDHKALGRAVMDLARSRAVSGAQVYFYGTRHPDVLVDISPVIDDKVRAVRAHRSQMVYLDEAETDGAVRLMARIMAGRSGVAYAEGLFRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SB3</accession>
    <name>Q67SB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH445"/>
    <dbReference type="EnsemblBacteria" id="BAD39430">
        <property type="protein sequence ID" value="BAD39430"/>
        <property type="gene ID" value="STH445"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH445"/>
    <dbReference type="HOGENOM" id="CLU_1365620_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269355" evidence="2">
        <location>
            <begin position="36"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="19987" checksum="01C4FAE5DD5BA610" modified="2004-10-11" version="1">MSVHGVVKRGRGSARVLRMGLTMLLIIGLVSGVPAHGSKLADVGAVEGNPVGQPDEESPSDPGGEDPDSVGQSDEEFPSDEGDEDSGSVGQPDEESPSGPGGEDPDSVGQPDEEFPSGGGDEDSGFVGQPDEESPSDPGGEGSDPGDVDPGDDEDGPAFPVAPGTEDDGGTDPAQEGLFAALAWCCPGPRWSPTTAAPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC8</accession>
    <name>Q67SC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194564.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH430"/>
    <dbReference type="EnsemblBacteria" id="BAD39415">
        <property type="protein sequence ID" value="BAD39415"/>
        <property type="gene ID" value="STH430"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH430"/>
    <dbReference type="eggNOG" id="COG2868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140910_2_2_9"/>
    <dbReference type="OMA" id="HAGYAEY"/>
    <dbReference type="OrthoDB" id="2065603at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16332">
        <property type="entry name" value="Prp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007422">
        <property type="entry name" value="Peptidase_Prp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036764">
        <property type="entry name" value="Peptidase_Prp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39178">
        <property type="entry name" value="PTHR39178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04327">
        <property type="entry name" value="Peptidase_Prp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118010">
        <property type="entry name" value="SSF118010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="109" mass="11410" checksum="A0FC3ABFFDF9F23F" modified="2004-10-11" version="1">MITVRVGRAEDGSVVSLETEGHAGYAEPGEDIVCAGVTALVVTALIGLKRVAGHPHEGKAVSGRAWCRLLPGGTPESRLKAQAILETTVLGLKDIAKDYKNYVRVTEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF2</accession>
    <name>Q67SF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194540.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH406"/>
    <dbReference type="EnsemblBacteria" id="BAD39391">
        <property type="protein sequence ID" value="BAD39391"/>
        <property type="gene ID" value="STH406"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH406"/>
    <dbReference type="HOGENOM" id="CLU_2014101_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13655" checksum="EBFFFFFEC3AE8E6E" modified="2004-10-11" version="1">MFWLQVKQFFVIALATVNLVLLIWALLAHRQRRVLPAGYYRLLPASAAIGLVQVSIGMFFLIQGRIPYWQHVLYGALVGVGAVLQFVLLPGTPAGQQYRNRPLVHAAVALFVALVGIRSWMTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK1</accession>
    <name>TIG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trigger factor</fullName>
            <shortName evidence="1">TF</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PPIase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tig</name>
        <name type="ordered locus">STH357</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194491.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SK1"/>
    <dbReference type="SMR" id="Q67SK1"/>
    <dbReference type="STRING" id="292459.STH357"/>
    <dbReference type="PRIDE" id="Q67SK1"/>
    <dbReference type="EnsemblBacteria" id="BAD39342">
        <property type="protein sequence ID" value="BAD39342"/>
        <property type="gene ID" value="STH357"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH357"/>
    <dbReference type="eggNOG" id="COG0544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033058_3_2_9"/>
    <dbReference type="OMA" id="KGIKTQF"/>
    <dbReference type="OrthoDB" id="932993at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00303">
        <property type="entry name" value="Trigger_factor_Tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="PTHR30560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003095">
        <property type="entry name" value="Trigger_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="SSF102735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00115">
        <property type="entry name" value="tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0697">Rotamase</keyword>
    <feature type="chain" id="PRO_0000179446" description="Trigger factor">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="1">
        <location>
            <begin position="163"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00303"/>
        </source>
    </evidence>
    <sequence length="429" mass="47932" checksum="5CD466CFCB0C0230" modified="2004-10-11" version="1">MKATWDKLENNWMQFEVEVDASEFSKSVEAAFRKMNRQVTIPGFRRGKAPRAVFERVYGKEALVQEAVDQILPRAYSEALEQGQIEPISQPEIEVVQAEDGKPLIFKGKVQVTPEVTLGRLSGFGIEKQVPEVTDQEVEEQLSALRERLATLVTDESGEVGQGSFAVIDFEGFIDGEPFEGGKGEGYTVEIGSGTFIPGFEEGLVGAKAGETREVTVTFPEDYHAQHLAGKQAVFKVTVHEVKKKELPELNDEFAQQVSPFKTVEELRADIKNRLSEAAAQKAEEDFRNKVVEAVADDASVEVPEVLVHDKVHDMIHDFEHQLAQQGITLDMWHQITGKTHEDLHKELEPQATKLVKVDLVLAAVAKQVGLTVSDAELEAEFDRLLAMYPKQQSYIRQLRASAAYRAQLRRDLLKQKTVEHLVELNSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN0</accession>
    <name>Q67SN0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH328"/>
    <dbReference type="EnsemblBacteria" id="BAD39313">
        <property type="protein sequence ID" value="BAD39313"/>
        <property type="gene ID" value="STH328"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH328"/>
    <dbReference type="HOGENOM" id="CLU_2653158_0_0_9"/>
    <dbReference type="OrthoDB" id="2055867at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024405">
        <property type="entry name" value="Phage_BhlA/UviB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10960">
        <property type="entry name" value="Holin_BhlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="76" mass="8786" checksum="697EB771B0ECDF60" modified="2004-10-11" version="1">MQAAGSEVRRMEEGLMKLAIEQGLWAALFVGLLLWTLRQNNARETRYLEIIKTLGEEVRARLDRLESLASRGRRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST3</accession>
    <name>Q67ST3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH275"/>
    <dbReference type="EnsemblBacteria" id="BAD39260">
        <property type="protein sequence ID" value="BAD39260"/>
        <property type="gene ID" value="STH275"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH275"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_2_0_9"/>
    <dbReference type="OMA" id="MESHRNF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="504" mass="57846" checksum="75A50AE5EE03F958" modified="2004-10-11" version="1">MKGLKIEMAHVERIRWLLHVEGKSQRQVAKALGISRKTVAKYAQLTEIPRYRRQKPAEPLVLTPEFQAEIERRLAENEILPRKQRWTGRTIYEDLVALGYQGSEPTVRRHIAKIRKLKRMQPAFIPLDHDPGESIEVDWGEAVVVLDGVEVTVHILCVRLRWSGMPVVIAYPTQRQEAMFDGLRRALELLGGVPRRMTLDNLKQAVKRILEGRNREEQDAFLSFRTYYLIDSNFCNPASGNEKGSVENLVGLAQRYIVGPHREARTWDELNAILWQRCLEYARRVPQGSTQSILERWEHEKKFLRPLPARPFDCCKVALVTSNKVSCVTFETCRYSVPVQYANRSLQVRAYWDRIDIYVGQEKIASHPRCYERNREILDLDHYLELLHAKPGALEQAKPFRMAQLPAVYHQFRAELQAQGRSDREFIEILMLHRTYSVSEVSTALERALAQRTACYAAVKQLLGAQPDLADGAPPSTAGELASIRVQQPSLAQYNRLLKGGVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY3</accession>
    <name>Q67SY3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 8">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">spo0A2</name>
        <name evidence="12" type="ordered locus">STH225</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8 9">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH225"/>
    <dbReference type="EnsemblBacteria" id="BAD39210">
        <property type="protein sequence ID" value="BAD39210"/>
        <property type="gene ID" value="STH225"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH225"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072509_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051606">
        <property type="term" value="P:detection of stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042173">
        <property type="term" value="P:regulation of sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014879">
        <property type="entry name" value="Spo0A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012052">
        <property type="entry name" value="Spore_0_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08769">
        <property type="entry name" value="Spo0A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002937">
        <property type="entry name" value="Res_reg_Spo0A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02875">
        <property type="entry name" value="spore_0_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="4 8" id="KW-0749">Sporulation</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="11">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002937-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="27696" checksum="8ABA59C2340E0FD1" modified="2004-10-11" version="1">MEGRTTVLIANDNHEERQRLMDKFDGMSEFTVVGEARHGLEAVELVRTLRPDLLILDDVLPHLDGLGVLAELGPEGRPHVLLLLSCASDLLVQHYYEKGASYCLLRPVQPDLVAERALLVCGHPAASQTSSAGRTVPSPRTIAELLRRSGVPAHLQGYRFLKDAVQYVLSTNKTTCGMTKELYPHIARLHGTQPNRVERSIRHAIEVAWGRADINDLQRLFGSTVHHVRGKPTNAEFVAMLADHLRGAVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T01</accession>
    <name>Q67T01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194342.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH207"/>
    <dbReference type="EnsemblBacteria" id="BAD39192">
        <property type="protein sequence ID" value="BAD39192"/>
        <property type="gene ID" value="STH207"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH207"/>
    <dbReference type="HOGENOM" id="CLU_2686479_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8035" checksum="6DFCEAEE33BEDCD8" modified="2004-10-11" version="1">MEKNRNRPEDALQGSVRSETQRVLSQLREGEAWSHRAVAMGYLAGGNEGQFLTADQSLGDAGDTQSPTDIDTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T41</accession>
    <name>Q67T41_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">UvrABC system protein A</fullName>
            <shortName evidence="14">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uvrA</name>
        <name evidence="17" type="ordered locus">STH167</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194302.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH167"/>
    <dbReference type="EnsemblBacteria" id="BAD39152">
        <property type="protein sequence ID" value="BAD39152"/>
        <property type="gene ID" value="STH167"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH167"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_2_9"/>
    <dbReference type="OMA" id="PFEGIIP"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 14" id="KW-0742">SOS response</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="598"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="738"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <begin position="639"/>
            <end position="646"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD39152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="954" mass="105836" checksum="4C5F80645BEB9628" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T53</accession>
    <name>Q67T53_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH155"/>
    <dbReference type="EnsemblBacteria" id="BAD39140">
        <property type="protein sequence ID" value="BAD39140"/>
        <property type="gene ID" value="STH155"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH155"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2002765_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13508">
        <property type="entry name" value="Acetyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="124" mass="14061" checksum="A3C7FB1E35968011" modified="2004-10-11" version="1">MAADLRTHHGFVAVRRTGAEEQVVGFVTWLPRVGDVNLVELTWLGVTPQFQRRGLGRRLVEAVADRCGQIGVSVMEVTTLADSVDYAPYARTRAFYRTLGFRDWRVDADFYGPGDDRLVLRKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T88</accession>
    <name>Q67T88_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative competence protein ComF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH120"/>
    <dbReference type="EnsemblBacteria" id="BAD39105">
        <property type="protein sequence ID" value="BAD39105"/>
        <property type="gene ID" value="STH120"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH120"/>
    <dbReference type="eggNOG" id="COG1040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054549_0_0_9"/>
    <dbReference type="OMA" id="RYNQSAL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044005">
        <property type="entry name" value="DZR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18912">
        <property type="entry name" value="DZR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DZR_2" evidence="2">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="152"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="27304" checksum="F69C7290574802FB" modified="2004-10-11" version="1">MTPLLRTILEGLVTLLWPQRTTCITCDGPLPAPLPPLATVAETVPVCADCWAAMPFTLDMRLCINCSRPLRGGWGLCAECVITPSYGRVWALGLHRGVLRAAVHHVKFSGRQALGEALGRYLAQMVEDLPDVIVPIPLHPIRERERGYNQAECIARGLAAELGVPVAADDLVRVRATGQQALRDRHARWRNLARAFGVRTGREPAWAGRHALLVDDVLTTGATASAAADVLWATGARAVNLAVVAVSDKPIPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE4</accession>
    <name>Q67TE4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH64</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194199.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH64"/>
    <dbReference type="EnsemblBacteria" id="BAD39049">
        <property type="protein sequence ID" value="BAD39049"/>
        <property type="gene ID" value="STH64"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH64"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_9"/>
    <dbReference type="OMA" id="LWIGPGQ"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="277" mass="29800" checksum="2A8C022C04281408" modified="2004-10-11" version="1">MEKLIITVALNGAEVTREQNPHIPFTPEEIAEDAARCRAAGAAMVHVHGRLPDGTPTQDAAVYKEILEAIRARTDVIVQVSTGGAVGMTAEERLAPVTLRPEMASLTTGTVNFGSDVFFNPPDLIETFARTMRQYGVVPEIEAFDVGHIDNALALVKKGILDLPLHFDFVMGVPGGIAGTIRNLLHMAESLPPGCTWSVAGIGRAQLPLATAAILLGGHVRVGLEDNIYYARGVKATNLMLVERIVRLANELGREVATPDEARRILGINREGDRDKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG8</accession>
    <name>PYRG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">STH40</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194175.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TG8"/>
    <dbReference type="SMR" id="Q67TG8"/>
    <dbReference type="STRING" id="292459.STH40"/>
    <dbReference type="EnsemblBacteria" id="BAD39025">
        <property type="protein sequence ID" value="BAD39025"/>
        <property type="gene ID" value="STH40"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH40"/>
    <dbReference type="eggNOG" id="COG0504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_9"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="783657at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="541"/>
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            <begin position="293"/>
            <end position="539"/>
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        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
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            <position position="386"/>
        </location>
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        </location>
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        <location>
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        </location>
    </feature>
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            <position position="13"/>
        </location>
        <ligand>
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        <location>
            <position position="13"/>
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        <ligand>
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        </ligand>
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        <location>
            <begin position="14"/>
            <end position="19"/>
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        <ligand>
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        </ligand>
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        <ligand>
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        </ligand>
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        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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            <position position="141"/>
        </location>
        <ligand>
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        </ligand>
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            <end position="150"/>
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        <location>
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            <end position="390"/>
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        </source>
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    <sequence length="541" mass="59746" checksum="7244E10797D8E1D2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG9</accession>
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        <recommendedName>
            <fullName evidence="6">RNAP delta factor</fullName>
        </recommendedName>
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        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
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        <lineage>
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            <taxon>Clostridia</taxon>
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        </lineage>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Asxl_HARE-HTH"/>
    </dbReference>
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        <property type="entry name" value="RNAP_delta_N_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RpoE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
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            <end position="159"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009828"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="159" mass="18146" checksum="F98AFBC610E9A57C" modified="2004-10-11" version="1">MGSPMELKPDMSVTDLAFTILKKHGKPMHFKELITEVMQVKAISQENPGRLIAQMHTEINLDSRFIHQGGGEWGLREWLPKTNRVIHIKQDGTPGRVSRPPLRLEDEIDEIGAEEEEELEELGEDLDDEYGLDDVDEELELEDIDVDVDEEPGEDEDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q3V816</accession>
    <name>Q3V816_SYMTH</name>
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        <recommendedName>
            <fullName evidence="3 9">Flagellar biosynthetic protein FliQ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">fliQ</name>
        <name evidence="10" type="ordered locus">STH2990</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
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            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Role in flagellar biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FliQ/MopD/SpaQ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197105.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2990"/>
    <dbReference type="EnsemblBacteria" id="BAD41973">
        <property type="protein sequence ID" value="BAD41973"/>
        <property type="gene ID" value="STH2990"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2990"/>
    <dbReference type="eggNOG" id="COG1987">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164516_2_0_9"/>
    <dbReference type="OMA" id="TMPWMLN"/>
    <dbReference type="OrthoDB" id="1939288at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002191">
        <property type="entry name" value="Bac_export_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006305">
        <property type="entry name" value="FliQ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34040">
        <property type="entry name" value="PTHR34040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01313">
        <property type="entry name" value="Bac_export_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004669">
        <property type="entry name" value="FliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00952">
        <property type="entry name" value="TYPE3IMQPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01402">
        <property type="entry name" value="fliQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="89" mass="9750" checksum="7C1745F705D60AF6" modified="2005-10-11" version="1">MTPEFALDLAQEAVRVALYVAAPVIGLSLLVGLIISLFQATTQVHEPSLQFVPKILAAVAGLVVFGPWMLTTLMDFARRVMDLLPRMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J62</accession>
    <name>Q67J62_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3307</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3307"/>
    <dbReference type="EnsemblBacteria" id="BAD42288">
        <property type="protein sequence ID" value="BAD42288"/>
        <property type="gene ID" value="STH3307"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3307"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_9"/>
    <dbReference type="OMA" id="LTFWAVN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="192"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="370"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="596" mass="65864" checksum="737763AD3DEE8C60" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J66</accession>
    <name>RLMH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.177</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (pseudouridine1915-N3)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m3Psi1915 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (pseudouridine-N3-)-methyltransferase RlmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmH</name>
        <name type="ordered locus">STH3303</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10221"/>
            <dbReference type="Rhea" id="RHEA-COMP:10222"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74486"/>
            <dbReference type="EC" id="2.1.1.177"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase RlmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.177" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197415.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J66"/>
    <dbReference type="SMR" id="Q67J66"/>
    <dbReference type="STRING" id="292459.STH3303"/>
    <dbReference type="EnsemblBacteria" id="BAD42284">
        <property type="protein sequence ID" value="BAD42284"/>
        <property type="gene ID" value="STH3303"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3303"/>
    <dbReference type="eggNOG" id="COG1576">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100552_0_0_9"/>
    <dbReference type="OMA" id="NEPYHHQ"/>
    <dbReference type="OrthoDB" id="1783583at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070038">
        <property type="term" value="F:rRNA (pseudouridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18081">
        <property type="entry name" value="RlmH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00658">
        <property type="entry name" value="23SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003742">
        <property type="entry name" value="RlmH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33603">
        <property type="entry name" value="PTHR33603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02590">
        <property type="entry name" value="SPOUT_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004505">
        <property type="entry name" value="MT_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000198198" description="Ribosomal RNA large subunit methyltransferase H">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="129"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00658"/>
        </source>
    </evidence>
    <sequence length="161" mass="17934" checksum="43DCF37049CB5E42" modified="2004-10-11" version="1">MRIRLVTVGKVKEKYLQDGVQEYLKRLRPYARVEIVTVPDEPIPDGASPAQEAQVMQREGERLLRALDQGGQEHVVVLDGRGKNFSSEELAAFLAERALHGDANLAFVIGGSLGLDPAVLARAGTTLSLGRMTFLHQMVPLILLEQIYRAFKINRGEKYHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J82</accession>
    <name>Q67J82_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ALP_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197399.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3287"/>
    <dbReference type="EnsemblBacteria" id="BAD42268">
        <property type="protein sequence ID" value="BAD42268"/>
        <property type="gene ID" value="STH3287"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3287"/>
    <dbReference type="eggNOG" id="COG4972">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066405_0_0_9"/>
    <dbReference type="OMA" id="TYMNQTY"/>
    <dbReference type="OrthoDB" id="894872at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10227">
        <property type="entry name" value="ParM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040607">
        <property type="entry name" value="ALP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042051">
        <property type="entry name" value="ParM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17989">
        <property type="entry name" value="ALP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ALP_N" evidence="1">
        <location>
            <begin position="6"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="38674" checksum="816DEBCD9128C8B5" modified="2004-10-11" version="1">MERLIGVDLGYGFVKATDGREGYLFPSVVGDGSPYLPLRLASQETDPTDNLRVQIGDRVYHVGTLAVRQSRMAYGFLSVMRDEGNDLLVLFLTALSLFASEANTTFSVVTGLPPGRMHLADQFVRSVRGDHRVVRYRTGNPEELYLRVDRVTVVPQPLGTYWSQVLDARGQLAQQHPAADARVGIVDIGFRTTDLVTVEGGEYVPEQSRTVPTGLSAAYGAVANALLREYGIERENHALDEAIISGEIGVSGRRVDITGLREKAFEQLATKVLVEIRSTWQVADYDFLWFTGGGGLALQRYLVPQFSQASLIADPLTANSREYLAWAHYIYGTGGAPWLERTPVNPQPRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB3</accession>
    <name>Q67JB3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signal peptidase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3255"/>
    <dbReference type="EnsemblBacteria" id="BAD42237">
        <property type="protein sequence ID" value="BAD42237"/>
        <property type="gene ID" value="STH3255"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3255"/>
    <dbReference type="eggNOG" id="COG1774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033149_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="426" mass="46384" checksum="DC763C91FD742B15" modified="2004-10-11" version="1">MVCVVGVRFKLAGKVYYFDPGELPVAQGDRVIVETVRGVEYGEVVVGPKMVPEDEVVQPLKKVIRIGNDRDAQIAEANKRKEKDAFAIALQKIEAHNLDMYLVDVEYTFDGSKIIFYFTAEGRVDFRELVRDLASVFRTRIEMRQIGVRDEAKLLGGIGPCGRVLCCTSFLGEFAPVSIRMAKDQNLSLNPSKISGLCGRLMCCLRFEQETYEAAKKDPTLAPAYVPPPLPDPEPEEMPEVEWLTEPAAPVPAEAVQPGAGDRARIAGAREDERPSRRERGRNRGEGTQEGNAVAQDETAEAGSAGGERKSRRRARRRSKRQQPRVDDSRQERSQAGGNREEQGRAEAGRGDHGRRDGSQAGEQSRRRGHGAREGGEPHGEAASAERPARSAHRRRRRRRPAAAVGGAAGGTASDGGAPAAGEAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JB4</accession>
    <name>Q67JB4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tetrapyrrole methylase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH3254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3254"/>
    <dbReference type="EnsemblBacteria" id="BAD42236">
        <property type="protein sequence ID" value="BAD42236"/>
        <property type="gene ID" value="STH3254"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3254"/>
    <dbReference type="eggNOG" id="COG0313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044779_2_0_9"/>
    <dbReference type="OMA" id="RTMVFFE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018063">
        <property type="entry name" value="SAM_MeTrfase_RsmI_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01296">
        <property type="entry name" value="RSMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD42236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="27579" checksum="28E46166708427C2" modified="2004-10-11" version="1">MIACEDTRETRKLLQHFQIDTPTTSYHEHNKRKAGPALIRRLLDGEDVALVSDAGMPAISDPGEELVRSALDAGIPVVPVPGPTALVTALVASGLPTGRFAFEGFLPHKGRERRRALERLKDEERTLILYEAPHRLLETLHDLRETLGDRPMTAARELTKLHEEYVRGTVSQVIDHFNRNAPRGEFVLVVQGAEAEAPVAGAPDPALLAGEVAAFVAQGMDRKLAMKEVAQRFGVSRKAVYQALLDAKDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD2</accession>
    <name>Q67JD2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gate domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3236</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3236"/>
    <dbReference type="EnsemblBacteria" id="BAD42218">
        <property type="protein sequence ID" value="BAD42218"/>
        <property type="gene ID" value="STH3236"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3236"/>
    <dbReference type="eggNOG" id="COG0370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682808_0_0_9"/>
    <dbReference type="OMA" id="HAVFEDT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="158" mass="17291" checksum="A82DB1B5E847913F" modified="2004-10-11" version="1">MWLWWLWEGLLTGVRQVAGLWWLILLFIGIQILKDAGWLGRASRYMAPVLRPLRLPGEAGVPMAAGLGIGLTYGAGVLIQTAREGRLTRDELTVMCVFLGICHAIFEETVLFAAAGANGLLLLAIRLAAAALFGWLAARAWLRPTEQASPVPDEVRTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-02" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH0</accession>
    <name>FTSH3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent zinc metalloprotease FtsH 3</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsH3</name>
        <name type="ordered locus">STH3198</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197311.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JH0"/>
    <dbReference type="SMR" id="Q67JH0"/>
    <dbReference type="STRING" id="292459.STH3198"/>
    <dbReference type="MEROPS" id="M41.021"/>
    <dbReference type="EnsemblBacteria" id="BAD42180">
        <property type="protein sequence ID" value="BAD42180"/>
        <property type="gene ID" value="STH3198"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3198"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_9"/>
    <dbReference type="OMA" id="QYGMTER"/>
    <dbReference type="OrthoDB" id="190468at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000400402" description="ATP-dependent zinc metalloprotease FtsH 3">
        <location>
            <begin position="1"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="29"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="125"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="626" mass="69572" checksum="2A2C9FD7A5F003F6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JN5</accession>
    <name>Q67JN5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Class III stress response-related ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3133</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197246.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3133"/>
    <dbReference type="EnsemblBacteria" id="BAD42115">
        <property type="protein sequence ID" value="BAD42115"/>
        <property type="gene ID" value="STH3133"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3133"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_1_9"/>
    <dbReference type="OMA" id="SKMMQGE"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="10">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="9">
        <location>
            <begin position="425"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="421"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="833" mass="92880" checksum="A7F75E6B5F295C24" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS6</accession>
    <name>Q67JS6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translocase subunit SecE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">secE</name>
        <name evidence="9" type="ordered locus">STH3092</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3092"/>
    <dbReference type="EnsemblBacteria" id="BAD42074">
        <property type="protein sequence ID" value="BAD42074"/>
        <property type="gene ID" value="STH3092"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3092"/>
    <dbReference type="eggNOG" id="COG0690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2304040_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33910">
        <property type="entry name" value="PTHR33910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13576" checksum="1C168CA1B27F6FF3" modified="2004-10-11" version="1">MTGTAVQRFPEAGDDRRKLCPRALQPVRGRATICTLVALPGFICVGAFSISRVVVIMERIKAFFEGIRTYLREVVGELRKVVWPSRERVIKATGIVVAMVALVAGFLYLWDLGIGALMELLLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JX6</accession>
    <name>RL13_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplM</name>
        <name type="ordered locus">STH3041</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JX6"/>
    <dbReference type="SMR" id="Q67JX6"/>
    <dbReference type="STRING" id="292459.STH3041"/>
    <dbReference type="EnsemblBacteria" id="BAD42024">
        <property type="protein sequence ID" value="BAD42024"/>
        <property type="gene ID" value="STH3041"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3041"/>
    <dbReference type="eggNOG" id="COG0102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_2_2_9"/>
    <dbReference type="OMA" id="DFVVIIN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000261805" description="50S ribosomal protein L13">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <sequence length="143" mass="16237" checksum="221303BFD8E7E414" modified="2004-10-11" version="1">MNKTYMANPQNVERKWYVVDAEGKTLGRLASKVAAILRGKHKPTFTPHVDCGDFVIIVNAEKIQVTGKKVTDKVYYRHSGYPGGQKATTFEQMIARRPERVLELAIKGMLPHNRLGRQMYRKLKVYAGPEHPHAAQKPEPLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K82</accession>
    <name>Q67K82_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197049.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2933"/>
    <dbReference type="EnsemblBacteria" id="BAD41916">
        <property type="protein sequence ID" value="BAD41916"/>
        <property type="gene ID" value="STH2933"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2933"/>
    <dbReference type="eggNOG" id="COG2071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030756_2_0_9"/>
    <dbReference type="OMA" id="PEYWAET"/>
    <dbReference type="OrthoDB" id="1628378at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="PTHR43235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="241" mass="26538" checksum="00295448654D5EAB" modified="2004-10-11" version="1">MRPLIGITACNRANEEPGQDWLYTPHDYFRAVWRAGGLPVMLPFVTDEEEAAQVLDRLDGLLLSGGGDLDPMLYGELPLPVTGSIEPERDQAELAYARVAVARDMAVLGICRGHQVLAVAFGGALWQDIPAQVEGAIKHRQEAPKNYPSHPVSILPGTRLAALLGTERRVNSRHHQAVKRVPEGWVASAHAPDGVIEAMEHPGRRFVLSVQWHPENHQGQPCNFDALFDAFVEAARRSVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K99</accession>
    <name>Q67K99_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="8">IMP dehydrogenase</shortName>
            <shortName evidence="8">IMPD</shortName>
            <shortName evidence="8">IMPDH</shortName>
            <ecNumber evidence="8 15">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">guaB</name>
        <name evidence="17" type="ordered locus">STH2916</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 15">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 14">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="8 15"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197033.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2916"/>
    <dbReference type="EnsemblBacteria" id="BAD41899">
        <property type="protein sequence ID" value="BAD41899"/>
        <property type="gene ID" value="STH2916"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2916"/>
    <dbReference type="eggNOG" id="COG0516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_9"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="OrthoDB" id="532857at2"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 8 15" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 15" id="KW-0520">NAD</keyword>
    <keyword evidence="6 8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 8 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="94"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="16">
        <location>
            <begin position="155"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8 10">
        <location>
            <begin position="339"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8 10">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8 10">
        <location>
            <begin position="386"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="8 9">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="249"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 12">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 12">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 12">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD41899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="486" mass="52428" checksum="B0611E606810260F" modified="2004-10-11" version="1">MDAKFGPEALTFDDVLLVPAHSEVLPRDVDVSTRLTRKIRLNIPLVSAAMDTVTEARMAIAMAREGGIGIIHKSMSIERQAEEVDKVKRSEHGIIVDPVFVHPDDMIETALQLMARYRISGTPVVERGTHKLVGILTNRDLRFEDNWNQPVGNVMTRENLITAPVGTTLEQAREILRHAKVEKLPLVDEHGVLKGLITIKDIEKAKKYPNSAKDEHGRLLCGAAVGVSDDLMERAGALVDAGVDVLVLDSAHGHSRGIMEALRKVKRNFPQVQVIAGNVATYEGTRDLIEAGADAVKVGIGPGSICTTRVVAGIGVPQITAIYESARAADEYDVPIIADGGIKYSGDITKAIAAGASAVMIGSLFAGTDESPGEMEIYQGRSFKVYRGMGSLGAMKQGSGDRYFQEGAPKLVPEGIEGRVPYRGPLAEMVFQLVGGLRAGMGYCGTRTIEELRKNGKFCRITAAGLRESHPHDVMITKEAPNYSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA2</accession>
    <name>Q67KA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2913</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197030.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2913"/>
    <dbReference type="EnsemblBacteria" id="BAD41896">
        <property type="protein sequence ID" value="BAD41896"/>
        <property type="gene ID" value="STH2913"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2913"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_9"/>
    <dbReference type="OMA" id="MDAYAIE"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="6"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="273"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="41493" checksum="F09C9E8CE6419E40" modified="2004-10-11" version="1">MGREAVIVSAARTPFGAFQGALKDLSATDLGAVAIREALRRVELLERPEEIDQVLMGQVVQAGAGQIPARQAAHKAGLPFGVVSATVNKVCASSLWAVNLADTLIRAGDADVVVAGGMESMSNAPYLLQGARRGYRMGHGQLIDGVIHDGLWCAFGDVHMGTYGGRGAREYGVAREELDAWAYRSHVRAAAAWDRGFFAEEVTRVVVPQKKGETVVLEDEPIRRDTSPEKLAALRPAFEPDGPITAGNAPGLSDGASALVIMSREKATALGLEVLATIVAQGQYSDEPHLLHEVPARAGQAALRRAGLTAKDLHLVEINEAFASVTLISTRLLGVDPEIVNVNGGAIALGHPIGASGGRILMHLVYELRRRGGGFGLAAICSGGGQGEATLVRVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB8</accession>
    <name>CH60_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60</fullName>
            <shortName evidence="1">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL</name>
        <name evidence="1" type="synonym">groL</name>
        <name type="ordered locus">STH2897</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197014.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KB8"/>
    <dbReference type="SMR" id="Q67KB8"/>
    <dbReference type="STRING" id="292459.STH2897"/>
    <dbReference type="EnsemblBacteria" id="BAD41880">
        <property type="protein sequence ID" value="BAD41880"/>
        <property type="gene ID" value="STH2897"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2897"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_9"/>
    <dbReference type="OMA" id="TDTDKME"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000063565" description="Chaperonin GroEL">
        <location>
            <begin position="1"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="540" mass="57898" checksum="503B3E32FD99A4B9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD2</accession>
    <name>Q67KD2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2883"/>
    <dbReference type="EnsemblBacteria" id="BAD41866">
        <property type="protein sequence ID" value="BAD41866"/>
        <property type="gene ID" value="STH2883"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2883"/>
    <dbReference type="HOGENOM" id="CLU_803924_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="37219" checksum="628D25A33BAB0D93" modified="2004-10-11" version="1">MPMQVVRAGSWQRWTVAGAALLLVLVYALQWLAGGGEIELHQVMNYFQVVSSMVIVTWVGDRRNRPTMLTGSVLRVARAFGWEAIPLAAAEVKPLRTGWQVRWNDGREVRRLSLVTPPEFREAVLRAAEQARAAPPGEAPRTVREALLAVPLAERSAVRGRGAAYLALLTVLPLLSLWLNHPWPLFGLPVLVWFQDRIFAGTTVVLSDRILWLLGAQGPVDQIPLERVRSVEGVKRNRVLLRLDDPRHPALKLFRYHEGVDVLAQIEKAMAGQPLFTPAGEGVSQAAAARAGDDPTAGEAVSPAGGGDHSHLRALPAPGPVRGAGGRPRPAGKGAAAHVRAAGSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH9</accession>
    <name>HIS4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="1">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisA</name>
        <name type="ordered locus">STH2834</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD41819" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41819.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_043714220.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KH9"/>
    <dbReference type="SMR" id="Q67KH9"/>
    <dbReference type="STRING" id="292459.STH2834"/>
    <dbReference type="EnsemblBacteria" id="BAD41819">
        <property type="protein sequence ID" value="BAD41819"/>
        <property type="gene ID" value="STH2834"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2834"/>
    <dbReference type="eggNOG" id="COG0106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_9"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="OrthoDB" id="794219at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142062" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="249" mass="26416" checksum="700B403F228495C9" modified="2005-02-01" version="2">MRLDLYPAIDLKDGQVVRLRQGRMDEATVYGVDPVRIAARWAEAGARWIHVVDLDGALRGRPQNAAAVRAIVEALRQRHPGVRVQLGGGLRTLEALEAALALGVSRAIIGTSALEGDVAARAVARFGPDRVAVSIDARGGFVAARGWVEVTRVRAVDLAVRMREVGVRTVVYTDIATDGMLTGPNFAELEMMGRTGLDVIASGGISSLEDIRRLTEIPGVAGAIIGRALYTGAVDLAEALSLCGETRDT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI2</accession>
    <name>HIS8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisC</name>
        <name type="ordered locus">STH2831</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196950.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KI2"/>
    <dbReference type="SMR" id="Q67KI2"/>
    <dbReference type="STRING" id="292459.STH2831"/>
    <dbReference type="EnsemblBacteria" id="BAD41816">
        <property type="protein sequence ID" value="BAD41816"/>
        <property type="gene ID" value="STH2831"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2831"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_9"/>
    <dbReference type="OMA" id="IMMGPGS"/>
    <dbReference type="OrthoDB" id="1248286at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153464" description="Histidinol-phosphate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <sequence length="361" mass="39538" checksum="A025E063F67B970F" modified="2004-10-11" version="1">MSSVRTAVRRMKPYVPGKPVEDVQRELGLHDLVKLNQNENPLGPSPRAVAAARAAMAQVHTYPEGTARRLRERLAQMWNLPADWFLIGNGSDEVFRLLAEVYLEPGDRVVVPEPSFAAYRFVAELMGAEVVAVPLAGWTMDLPAMAEAAARGAKLLFLCRPNNPTGTVFAEADLRAALERVPPSTLVVVDEAYREFDETPFDSRALVQDYPNVVIARTFSKIYGMAGFRLGYGVMRPEVLAPLYTARDPFSVNGLAVAAGLAALDDVEHVERTRALTREGKAYLYAAFQRLGLGYVPSEANFVLFDAGRPAAEVFDALLRRGVLVRPCGSFGLPDHLRVTVGTPEQNRRFVEALKAALGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK9</accession>
    <name>Q67KK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Somatin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196923.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2804"/>
    <dbReference type="EnsemblBacteria" id="BAD41789">
        <property type="protein sequence ID" value="BAD41789"/>
        <property type="gene ID" value="STH2804"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2804"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053998_0_0_9"/>
    <dbReference type="OMA" id="NLMVVLC"/>
    <dbReference type="OrthoDB" id="1213601at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="57"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="287" mass="31289" checksum="9367F5C28A7361EB" modified="2004-10-11" version="1">MVKALPGWIGLVTVLLLIGLSIWLFALGLPDAPWLRPSPVLLAASAVLFLVACICCNGLFVVQPNQARVLVLFGRYTGTVKADGWYFVNPLVSKRPVSLRVRNFTSPQLKVNDANGNPIEIAAVVVWRVVDTARAVFSVEDYNAFVEVQSETAIRHLASQYPYDDGLNEGELSLRGSAEEVALALKKELQDRLEMAGIAVIEARISHLAYSPEIAGAMLQRQQAAAIIAARQKIVEGAVSMVEMALDMLDQHRKVELTPERKAELVANLLVVLTSDRAATPTLPLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN5</accession>
    <name>Q67KN5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196897.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2778"/>
    <dbReference type="EnsemblBacteria" id="BAD41763">
        <property type="protein sequence ID" value="BAD41763"/>
        <property type="gene ID" value="STH2778"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2778"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064956_1_0_9"/>
    <dbReference type="OMA" id="TYENMHF"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="236" mass="26607" checksum="CF10B750B6AE8735" modified="2004-10-11" version="1">MVRTILFDLDGTLLPIDTDAFIRGYMQALAEYVGHIIPPEQLVKEVWAATGAMVRNTDPTLTNAQVFARAFFPAVGHDEAALMPVFEEFYRTRFPELRAACPGLPGMARSVVEAALEKGYEIVLATNPLFPRLAIEERMRWIEVHDLPWRLVTAYEEMHACKPQPEYFLEVLERIGRRPEECLMVGNDVQEDGAAARAGIDVFFITDFLIDREGRPLPPGRSGSLAEFRERLVTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP5</accession>
    <name>Q67KP5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH dehydrogenase I subunit M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nuoM2</name>
        <name evidence="8" type="ordered locus">STH2768</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Membrane</location>
            <topology evidence="1 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196887.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2768"/>
    <dbReference type="EnsemblBacteria" id="BAD41753">
        <property type="protein sequence ID" value="BAD41753"/>
        <property type="gene ID" value="STH2768"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2768"/>
    <dbReference type="eggNOG" id="COG1008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_4_4_9"/>
    <dbReference type="OMA" id="ITRWGNQ"/>
    <dbReference type="OrthoDB" id="535707at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000260">
        <property type="entry name" value="NADH4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010227">
        <property type="entry name" value="NADH_Q_OxRdtase_chainM/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="PTHR43507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01059">
        <property type="entry name" value="Oxidored_q5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01972">
        <property type="entry name" value="NDH_I_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_q5_N" evidence="7">
        <location>
            <begin position="38"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="6">
        <location>
            <begin position="136"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="508" mass="55430" checksum="6DC8DBB6619C9A68" modified="2004-10-11" version="1">MLSLIIFVPLVFALVMLFFVPKTQERLLYWLAGIGTLIPFALALVLAAQWPTAEVSTAPYAQGMRFVTHVPWIQAIGVHYTLGVDGFAFTMLLLSTLLFPLAVLASYGYIKDRVKDYLVLLLILATGTNGVFATLDFMLFYVFWEIVLLPMYFLIGIWGGERREYAAIKFFLYTLIGSTVMLVGAIYLYVATGVGTFNLLQMAAASSKLPITGLTTFAFFALLVGFAVKVPMFPFHTWLPDAHVEAPTPISMLLAGILLKLGSYAMVRISHPMLPQLATKYADLLFVFGVVGILYAALAAMAQKDFKKQVAYSSVNHMGFFIMALAAASKLAPTSPELAQVALQGGYFVTIAHGLISPLFFFAVGMFYERTHTRDLRKLGGMFLTIPVVSFATAFVAFANLGLPGLAGFIGEFISLTGSYPAFGLWVLVAGLGMVITAAWHLLTMRNVLLGEAKPEYQGLADITLKEKIIFAPLAVLIVFFGVYPRPVMALLQSSVEVLTQVMGLGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KS3</accession>
    <name>Q67KS3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="6 16">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">STH2739</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="6 16"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2739"/>
    <dbReference type="EnsemblBacteria" id="BAD41724">
        <property type="protein sequence ID" value="BAD41724"/>
        <property type="gene ID" value="STH2739"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2739"/>
    <dbReference type="eggNOG" id="COG0460">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_0_9"/>
    <dbReference type="OMA" id="LMFYGPG"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016204">
        <property type="entry name" value="HDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 16" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="18">
        <location>
            <begin position="352"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="BAD41724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="436" mass="47354" checksum="E0FC030CF1C3B763" modified="2004-10-11" version="1">MEKQTMRFAILGLGTVGKGVVKLLQESREMLHLKTGLNLELAKVLVRDASKPRPGFEGLPLTEDVSEILDDPSIRIVVELMGGIEPARTYLVEALRRGKTIVTANKDVMADYDKDLYDAGEIGDAEIYFEASVGGGIPIIRPLKEALAANTMRQVMGIVNGTTNYILTQMSQYGRSYEEALQEAQALGYAEPDPTNDVQGFDAARKLAILCTICFLSRVKPGMVYTEGITRVTPRDIEYGRRFGWVLKLLAIGKEVDGEIEARVHPAFIPHTHPLASVAGSFNAIYTVGDPVGETMFYGRGAGAGPTASAVVSDLIAAALSKRTPGRRAGDAHTVYFEKRVLPIEETRSRYYLRLHAVDAPGSLARIAERFGEHGVSLAEVVQASGGPDHPDDGGAAELVLVTHTVQDANLQAVVRDLKEMRDTVLRVDNVIRVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT8</accession>
    <name>Q67KT8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2723</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2723"/>
    <dbReference type="EnsemblBacteria" id="BAD41708">
        <property type="protein sequence ID" value="BAD41708"/>
        <property type="gene ID" value="STH2723"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2723"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_4_9"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="42"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="360"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="393"/>
            <end position="400"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="599" mass="66611" checksum="32A276C98D382114" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV0</accession>
    <name>Q67KV0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2711</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2711"/>
    <dbReference type="EnsemblBacteria" id="BAD41696">
        <property type="protein sequence ID" value="BAD41696"/>
        <property type="gene ID" value="STH2711"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2711"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_9"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="9">
        <location>
            <begin position="3"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="36120" checksum="5E07FACDE4D889A3" modified="2004-10-11" version="1">MVGGAGYVGSHVVRALLEAGHEPVVYDNLSTGHMEAVCDSEVIIGDVGDRDKLRTVLQARRFDGVVHLAAASLVAESMREPSVYFRNNVSASIVLFDELIRADVPWVVLSSTAAVYGEPEVVPIPEEHPTRPTNPYGESKLMLERILKWYDVAYGFKHISLRYFNAAGAHPSGQIGEDHMPETHLIPIVLQVALGLRDFVTVFGIDYPTPDGSAVRDYVHVCDLADAHVRAITRLCATGTSSVYNLGSARGYSVREIVDVAQQVVGMPIKSVVADRRAGDPPILVADNRKALSELQWMPQFDLEAIVETAWRWHRDHPQGYGNRRSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV1</accession>
    <name>Q67KV1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2710"/>
    <dbReference type="EnsemblBacteria" id="BAD41695">
        <property type="protein sequence ID" value="BAD41695"/>
        <property type="gene ID" value="STH2710"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2710"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2144588_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="112" mass="12499" checksum="B9082F2689F9979A" modified="2004-10-11" version="1">MECHVRRDILRKGDALDNVVAESFYSTLQTELLNQSIWPTLASLRLSILEVFYIAADVTQRWATSHQQTLKRHGLSVKAAETASEPNCRVYEIETTLGVTELTHGARGSALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV7</accession>
    <name>Q67KV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2704</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41689">
        <property type="protein sequence ID" value="BAD41689"/>
        <property type="gene ID" value="STH2704"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2704"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_7_3_9"/>
    <dbReference type="OMA" id="ETAFFRF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="331"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="494" mass="53192" checksum="1E1BA8B241D31B69" modified="2004-10-11" version="1">MLIRHSFLYLGAHGVPVLIRVATLSVLTWLLTPGEYGIYTMVVAVVGMTNAVGFHWLRVSLLRFLPSAQGEGGRGALLSTIAVAYCIEVVVLSVLGGLAAIIFGFPQWILVTLLLLWTQAWLELEVEVTRVQLNPVKYGVLLTTRALLGLTLSSGLAYLGGGAIGALWGLVIANLVPAAWSYWRNRYMWVVRGFDWRVMRQIWTFGLPLATTSTLNYGVSTVDRFILGFMVGAEAAGLYAVAYDLPFQSLGALMTVTNLAGMPLAIRTLENEGIAAARRQLSANCVVLLGLGVPATLGIILLAPNVAGVFFGPEFQMDVARLIPVIAVGTLLEGVKTYYLDQAFQLAKNTLAQVGVAVLTAISKVGLTIILVHHFGLVGAAYSTVISFLIAAMLSWGVGRNIFAMPFPVGSLVKIVVATLIMGWLIAPLARYRGPLALGGQVIAGVLVYMCTAVLLNVGELRVHLINTIETWRLHRRSKATGSYSLGSDHKNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY8</accession>
    <name>Q67KY8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196795.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2673"/>
    <dbReference type="EnsemblBacteria" id="BAD41658">
        <property type="protein sequence ID" value="BAD41658"/>
        <property type="gene ID" value="STH2673"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2673"/>
    <dbReference type="eggNOG" id="COG1725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_874147_0_0_9"/>
    <dbReference type="OrthoDB" id="1860081at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="33375" checksum="6BBECF6637A2C49E" modified="2004-10-11" version="1">MQAFRLNRSSCVPLKEQLQAQIRYQIAAGLLHPGDQLPSLRDLAAGLAINVNTVARAVEELIGEGYLTSHQGKGIFVADEPPGQAPGAALRSLLSGVLATAQEWGMDAEELALDLLSQAQLARSPAPAANRVVLVATARSDLRRLQRQLESQLPGTQVAAVLPEELGHVPPGIPVVTTLFHGPALRRWQPVVLAGAESRGALDRLARLPAGSCVAVAASDRVQAARIQQSLEQGGFGHLRFRAVTAPDEVDALLGDSACLLAAPSGYPLAEAARAARPGLPCIREPLATPPEALTSLREALGGAAAPPRRVAIRSSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L01</accession>
    <name>Q67L01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginase-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196782.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2660"/>
    <dbReference type="EnsemblBacteria" id="BAD41645">
        <property type="protein sequence ID" value="BAD41645"/>
        <property type="gene ID" value="STH2660"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2660"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_6_1_9"/>
    <dbReference type="OMA" id="DTPFQIV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="PTHR43782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="313" mass="33711" checksum="492216B02A0C37F3" modified="2004-10-11" version="1">MTIHVLGFPTTLGLPRHAVRHAPEALRATGLLDFLGQLDSRVIDHGDMAVPPGLRSDPVPVRIAKVVEAARRQQQHWQRVARPGDLLFTIGGDHSTSLGTIMALAALGYDFDVVWVDAHGDFNTVETSPSGNPHGMVLALATGLLPDAMDGVIRPDRLRLWGIRDLDPGERRLLSEARVEVVSPAEVRARRAELLAGLRPNVFVSFDIDSVDPAEAPGTMTPVPGGFTRDEAVSLVADIVRGRNLLALDVVEFHPDRDEGDATGRLAHAVIRAALEAWRGQSPIRSGSPCASRSDADSRPSACASPTRRLYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L55</accession>
    <name>Q67L55_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196728.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2606"/>
    <dbReference type="EnsemblBacteria" id="BAD41591">
        <property type="protein sequence ID" value="BAD41591"/>
        <property type="gene ID" value="STH2606"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2606"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074359_0_0_9"/>
    <dbReference type="OMA" id="NIVIDWT"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="177"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="326" mass="35702" checksum="862FA66CC1216C52" modified="2004-10-11" version="1">MQNQPLTLDLLPQAVDLWNRTLGDLFPMRPELMASNLWNEPSFDPEGSRAILAGGRMVALVAVKRRQHPMHTLPADQQGWISALVVDPAFQGKGVGSALLRDALTHLGRYAAEPVALGSDPGHFFPGIPLDCRRALDWFARRGAQLGALECDLANTEMATYEHPEAARRAFAREPGIIYRPMAPGEEEAALAFMRRAFPGRWAWELERHLAGGGLREDVMLALENSAIVGFARIAGPWSRHFSPGIYWAPRFQGPHGSLGPIGVAPEARGRGIGLALLSASLAELRDRGVRSAVIDWTQLVRFYGIAGFRPWMWYVRATMPAGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L67</accession>
    <name>Q67L67_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2594"/>
    <dbReference type="EnsemblBacteria" id="BAD41579">
        <property type="protein sequence ID" value="BAD41579"/>
        <property type="gene ID" value="STH2594"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2594"/>
    <dbReference type="eggNOG" id="COG4997">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142081_0_0_9"/>
    <dbReference type="OMA" id="MPTYNKL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd11532">
        <property type="entry name" value="NTP-PPase_COG4997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038735">
        <property type="entry name" value="MSMEG_1276-like_NTP-PPase_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="110" mass="13056" checksum="AD9FF38AE6B392D0" modified="2004-10-11" version="1">MPGGRKRMTVYHKLVRDRIPEIIEASGRRCVVRVLDDQAYLDSLHRKLEEELQEYLTSRSLEELADLLEVAYAIAEFRGVRPEQLEQVRREKREARSGFRRRLFLLQVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA9</accession>
    <name>Q67LA9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thiol-disulfide isomerase and thioredoxins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2552</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196675.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2552"/>
    <dbReference type="EnsemblBacteria" id="BAD41537">
        <property type="protein sequence ID" value="BAD41537"/>
        <property type="gene ID" value="STH2552"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2552"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_4_9"/>
    <dbReference type="OMA" id="QMEEITQ"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269421" evidence="3">
        <location>
            <begin position="39"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="64"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="21879" checksum="93B80A9A3023F20F" modified="2004-10-11" version="1">MSVSAKPGAARRPAQAVVLLVLALLLAGCGGLQRDAGAAGAGSRSGAQAGGSAADQGTVLEVKPVPGYLAADIRAKDVFTGEVLTLSDLKGTPVLLNFWATWCGPCREEMPEMEEFHQEMGDAVRVVAVSADSGDSPEKMAAFAEALGLTFSVVHDGGSAARAYRVGAIPTSFFIDGEGVIQVRYTGPLTLEQMKEYAELASNTAEDPEHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI6</accession>
    <name>Q67LI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2475"/>
    <dbReference type="EnsemblBacteria" id="BAD41460">
        <property type="protein sequence ID" value="BAD41460"/>
        <property type="gene ID" value="STH2475"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2475"/>
    <dbReference type="HOGENOM" id="CLU_3277683_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="41" mass="4449" checksum="0316FBB080E99571" modified="2004-10-11" version="1">MLDVGEIGVRDVSIVTAGALKPLAQVQALPRLLRLHPDMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI7</accession>
    <name>Q67LI7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196597.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2474"/>
    <dbReference type="EnsemblBacteria" id="BAD41459">
        <property type="protein sequence ID" value="BAD41459"/>
        <property type="gene ID" value="STH2474"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2474"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091584_0_0_9"/>
    <dbReference type="OMA" id="ITAGVCE"/>
    <dbReference type="OrthoDB" id="1623593at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="240" mass="25562" checksum="4C5DDBB5C680EF9E" modified="2004-10-11" version="1">MPADVLAGVALAVQSAIALYLVVGEPILGTRSYRRLLAILDSDPAARLNFYRKIVILEWALVFLVLVTLRLAGEPAAVIGLTSGRLDSEALILVIALAAGIAAPLIIGAFSPGYREALQRQMESVKGLLPGRPDERLWYALVAITAGVCEEILFRGFLIAYFMKVVPGLPVVAALILSGAVFGMAHLYQGWGGVMTTGFLGIMLGVLYLYTGSLVWPIVTHALLDLRVLALPGVKPPASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP1</accession>
    <name>Q67LP1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196543.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2420"/>
    <dbReference type="EnsemblBacteria" id="BAD41405">
        <property type="protein sequence ID" value="BAD41405"/>
        <property type="gene ID" value="STH2420"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2420"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_19_8_9"/>
    <dbReference type="OMA" id="HREATAN"/>
    <dbReference type="OrthoDB" id="1809950at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="18746" checksum="C61C0C11E0A4D9FB" modified="2004-10-11" version="1">MDQIRIRMARPSHAEAFAQIMTQPKVYYGTLQLSHTTPESWQKRLESNDPQHDYVLVAEVEGKVVGNLGLHRSRHPRTLHVASLGMSVHDAHQGQGIGRALMAAAIDAADRWLNILRIELEVYPDNERAIKLYESFGFVVEGRKRMAAFRDGQYVDTLVMGRIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU8</accession>
    <name>Q67LU8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2363</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196486.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2363"/>
    <dbReference type="EnsemblBacteria" id="BAD41348">
        <property type="protein sequence ID" value="BAD41348"/>
        <property type="gene ID" value="STH2363"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2363"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_9"/>
    <dbReference type="OMA" id="YKQDGVG"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="38735" checksum="D37B9DE3401F6836" modified="2004-10-11" version="1">MKLDTVLVHAGVRRDPAYGAVSVPVYQSATFQHPALGESTGYDYSRSGNPTRAALEEALARAEGGARALAFASGMAALTCALGLFGPGDHLVVTEDLYGGTYRLLEQVLALPHTYADTADLDAVRAAIRPDTRAVLVESLTNPRMKRADVAALAGLCRAHGLLLIVDNTFLTPWLCRPLELGADIVVHSATKYLAGHNDVVAGALITRDTALGDRLSFLQNAVGSVLGPQDSWLVLRGLKTLALRMERHQQNAARIAAWLREHPRVEEVLYPGVGGMLSFTVTEPSLVPQVLRRVKLILFAESLGGVESLITFPWTQTHADMPEEVRRRLGITDRLLRLSVGIEDADDLIADLAQALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV0</accession>
    <name>Q67LV0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched chain amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2361"/>
    <dbReference type="EnsemblBacteria" id="BAD41346">
        <property type="protein sequence ID" value="BAD41346"/>
        <property type="gene ID" value="STH2361"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2361"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_2_9"/>
    <dbReference type="OMA" id="KYIFRFV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269260" evidence="3">
        <location>
            <begin position="30"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="50"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="395" mass="42523" checksum="97FD948EF6FC4A86" modified="2004-10-11" version="1">MSLRGAQRARFWLAWVLAFSVVLAGCSSAATAPSGAQGSAGSQNGAQGEVLIGVMGPLTGDSAEYGKIWRRGFDLALEEINAAGGVQGRPLNYVFEDTQSDPKQSPAVAQKFVNDPRVVAVIGDFSSPASMAASPIFQRAGLVQLGITNSHPDFTKTGDFIWSNSTSQTDEAPFLARLAVEQLGRKRLAVLHQNTDWGKTTADLFVEQARALGAEVVLQEAYLLTEKDFRPVLTKARDAQPDALVLISYYNDGALIAQQIPAVGLDVTVLAASSVYSPQFLNLGGAAVEGVYTVSRFHPADPRPEAQQFVERFRERYGTDPDTFAAGAYDGMKILAAVLNRVGVDRKALRDGLEQAEHLETILQGATAFGEDRRLRNPRFVPLVVRGGAFAIWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV8</accession>
    <name>Q67LV8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2353</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196476.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2353"/>
    <dbReference type="EnsemblBacteria" id="BAD41338">
        <property type="protein sequence ID" value="BAD41338"/>
        <property type="gene ID" value="STH2353"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2353"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446825_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HD-GYP" evidence="7">
        <location>
            <begin position="284"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="545"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="486"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="611" mass="63882" checksum="436EB7A23D899E4A" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M04</accession>
    <name>Q67M04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2307"/>
    <dbReference type="EnsemblBacteria" id="BAD41292">
        <property type="protein sequence ID" value="BAD41292"/>
        <property type="gene ID" value="STH2307"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2307"/>
    <dbReference type="eggNOG" id="COG0420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_0_1_9"/>
    <dbReference type="OMA" id="FVYAMKW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="373" mass="41017" checksum="32226F6F6A69E333" modified="2004-10-11" version="1">MRILHLADLHLGWRPAFMAREQAEERRRRRDGLLARAVDLALDPGRRIGLVVIAGDLFETHCPEEPLVAAAVAQLRRLEQAGIPVVTVPGNHDEITYPNSVYQRRRDGWPGLLVTNPHPAHVATIEAQGETVHLYSLAYTGGVTQTRPAIRQFPRLDQPGLHVAIFHGTLGEGLGDRSLPLDPDALGRAGYDYIALGHIHKPSEVRLGRGLAVYPGTVEGKGFDDPGTRQFTIASVEPGGVTLERVPDPAAQKVAVHDLDVSPLPGAARVEAAVAALADPEAIVRVRLTGTAPELLDAEAIEAACAHRFFHLEVVDETDALSPALLDRWAAERTILGEFIRRMRRRMEEEQDEDERAVLEEALRRGVQAMRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M35</accession>
    <name>Q67M35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2275"/>
    <dbReference type="EnsemblBacteria" id="BAD41260">
        <property type="protein sequence ID" value="BAD41260"/>
        <property type="gene ID" value="STH2275"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2275"/>
    <dbReference type="eggNOG" id="COG3666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_1_1_9"/>
    <dbReference type="OMA" id="YLAANTH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF772" evidence="1">
        <location>
            <begin position="62"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="164" mass="19041" checksum="BC95102F527FB68F" modified="2004-10-11" version="1">MSNVFSVTKKTYRPYEPNQLLLLPPALQDWLPHDHFTYFLSDVVDSLDLSQIEQTYERELRGYPPYHPRMMVKVLLYAYCNGVYSSRKIERRLQEDVAFRVLAANNQPDFRTISDFRKRHLKALSDLFLQVVKICKKAGLVRLGHVALDGTKIKANASKHKAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M53</accession>
    <name>Q67M53_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2257"/>
    <dbReference type="EnsemblBacteria" id="BAD41242">
        <property type="protein sequence ID" value="BAD41242"/>
        <property type="gene ID" value="STH2257"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2257"/>
    <dbReference type="eggNOG" id="COG3183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1030259_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH" evidence="1">
        <location>
            <begin position="184"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="30927" checksum="BB49EB4ECE891FF6" modified="2004-10-11" version="1">MHWLGVRLVEAAKEGHTVYYHELSKPLQLTNYRLLSEPLGELSELAMDNGFPPISAVVINVTDQLPGAGFFKLVGRKLRGYEIPEQEWQPFFAEMLAEVFAWDDWDDFLEVLDETYGSDLPATRAAGTGRTAHPDLARTVEQDLQAEEIEEGRIEGGVVEYYGRRYERDPENRRRAIEIHGLNCVVCGFNFEEVYGPRGRDFIEVHHVKPLKAFEGVAQVVDPKVDLVPICANCHRMIHRRHDSVLTPDQLRRIVEEQRATRSPGHRPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD1</accession>
    <name>Q67MD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2177"/>
    <dbReference type="EnsemblBacteria" id="BAD41162">
        <property type="protein sequence ID" value="BAD41162"/>
        <property type="gene ID" value="STH2177"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2177"/>
    <dbReference type="HOGENOM" id="CLU_3158619_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="48" mass="5433" checksum="AA209861F47554EC" modified="2004-10-11" version="1">MHRGLPLSRRSREDGVNLWMRRCGRLRGRAVGGTPAPGQQDQPRPQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF1</accession>
    <name>Q67MF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobalt ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2157"/>
    <dbReference type="EnsemblBacteria" id="BAD41142">
        <property type="protein sequence ID" value="BAD41142"/>
        <property type="gene ID" value="STH2157"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2157"/>
    <dbReference type="eggNOG" id="COG0619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_2_3_9"/>
    <dbReference type="OMA" id="CYILPEM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="26700" checksum="580EA7D15B515B60" modified="2004-10-11" version="1">MPGDSWVHRLDPVSKLLLAAVGLALTFVLPGHLAPALLLAAALAILATARVLRRSGAVFAGVLLIAVTFFIVQGLVYAGNATPALTLGPVTFYREGLLLGLRLTLRLYNILASSLILILTTRPSDLVESLMRRGLPSRFGYVIIAVLQVIPVMVEAAGRITDAQRSRGMETEGSLWVRLRAFIPLMGPLVTSSLIATEERALALEVRGFASNRKLTFLRDEIIPGYAWPVRIAALAALALGIAGRVMLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT5</accession>
    <name>RF3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor 3</fullName>
            <shortName evidence="1">RF-3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prfC</name>
        <name type="ordered locus">STH2023</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD41008" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41008.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MT5"/>
    <dbReference type="SMR" id="Q67MT5"/>
    <dbReference type="STRING" id="292459.STH2023"/>
    <dbReference type="EnsemblBacteria" id="BAD41008">
        <property type="protein sequence ID" value="BAD41008"/>
        <property type="gene ID" value="STH2023"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2023"/>
    <dbReference type="eggNOG" id="COG4108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_2_1_9"/>
    <dbReference type="OMA" id="GFVFKIH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04169">
        <property type="entry name" value="RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00072">
        <property type="entry name" value="Rel_fac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004548">
        <property type="entry name" value="PrfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032090">
        <property type="entry name" value="RF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038467">
        <property type="entry name" value="RF3_dom_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041732">
        <property type="entry name" value="RF3_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43556">
        <property type="entry name" value="PTHR43556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16658">
        <property type="entry name" value="RF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00503">
        <property type="entry name" value="prfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242219" description="Peptide chain release factor 3">
        <location>
            <begin position="1"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="9"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="528" mass="59895" checksum="70CE1023AC0B124B" modified="2006-06-27" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N35</accession>
    <name>Q67N35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1923</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1923"/>
    <dbReference type="EnsemblBacteria" id="BAD40908">
        <property type="protein sequence ID" value="BAD40908"/>
        <property type="gene ID" value="STH1923"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1923"/>
    <dbReference type="eggNOG" id="COG1430">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_4_0_9"/>
    <dbReference type="OMA" id="HIVPCNS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="112" mass="12500" checksum="65BC07A8D5939FB2" modified="2004-10-11" version="1">MIVRNLTRGLVLGDRIRRADTFRLRLLGLMFRPGLEPGEGLWLEPCCQVHTHFMRFPLDLLFLDAEGRVLRALASFPPRRISPAVPGARAVLELPAGAMGETRPGDLVRAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N43</accession>
    <name>Q67N43_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1915"/>
    <dbReference type="EnsemblBacteria" id="BAD40900">
        <property type="protein sequence ID" value="BAD40900"/>
        <property type="gene ID" value="STH1915"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1915"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1668510_0_0_9"/>
    <dbReference type="OMA" id="GWANDPD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="158" mass="17444" checksum="F24CB69B31CAF97E" modified="2004-10-11" version="1">MLVKAPQEVVWWAWTRAERITQWFAPAATIDPRPGGAFELFFNPADRSRDCTAGCTFTAVEPMTRLAFTWKGPDLFAAVMNHPDRLTAVTVTMEPVEGGTRVTVVHAGWGEGDGWAEARRWHAAAWRQVLDSLKGALESGRGDLCCAPDEDQNQATVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N66</accession>
    <name>Q67N66_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetoin dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1892</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196019.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1892"/>
    <dbReference type="EnsemblBacteria" id="BAD40877">
        <property type="protein sequence ID" value="BAD40877"/>
        <property type="gene ID" value="STH1892"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1892"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_6_0_9"/>
    <dbReference type="OMA" id="CAHPLDF"/>
    <dbReference type="OrthoDB" id="2014094at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="73"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="134"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="205" mass="23126" checksum="79371D982ACE2438" modified="2004-10-11" version="1">MIVRNVMTEDVTTVSPDDTLQQAYEIIQTKNYDCLPVTANRRVVGIIQLTDIYEACMRDGRQAALPRPVKEFMVPDPVTVRPDDLVETAAKLMFKRDIPLLPVVHGERLVGVIHEHDIFRAFAHLLGVDSGTIRLTLVVPDRVGQLARIAELIRDAGVAIRNVATFRSSVLDQYQIIIRVETEASRPLIDLLERHGYKVLHVLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA5</accession>
    <name>Q67NA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1853</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1853"/>
    <dbReference type="EnsemblBacteria" id="BAD40838">
        <property type="protein sequence ID" value="BAD40838"/>
        <property type="gene ID" value="STH1853"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1853"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_2_2_9"/>
    <dbReference type="OMA" id="RDYDFLQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="95"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="285" mass="29466" checksum="CB81F6E93D7E84EE" modified="2004-10-11" version="1">MALLAQAGAVAPAPAAAGPEPSAPEVRVVPPPSAPKDAPADPSAAVAPGGQDGSAPADAEAKPAGGPGEEPAGQPPAPAGPVSPDPLIRLPTEAKEVVLTIDDGPGPLTERFLAVLEAEEVPAVFFWLSGSRSLPLAAEVVRQGHQIGTHTVSHPRLLQLQPGEAAAQITESQAALEAAAGVPVRFFRPPYGEHDRQIREIAAARGMSTVLWTVDSRDWALADDPEQIVANVLQQVKPGALILIHERAQTLAVLPDLIRALREAGYTFIPLPEPDPLPRAVASDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA8</accession>
    <name>Q67NA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195978.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1850"/>
    <dbReference type="EnsemblBacteria" id="BAD40835">
        <property type="protein sequence ID" value="BAD40835"/>
        <property type="gene ID" value="STH1850"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1850"/>
    <dbReference type="HOGENOM" id="CLU_192686_1_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018730">
        <property type="entry name" value="DUF2273"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10031">
        <property type="entry name" value="DUF2273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8800" checksum="6BF681A8A37846EF" modified="2004-10-11" version="1">MKWEWLKEVAAEHRYKIIGGLGGFLFALLVVWFGWWALFILFCTGIGYWIGKRMDEGPESLVQIFERLLPPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF7</accession>
    <name>Q67NF7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195929.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1801"/>
    <dbReference type="EnsemblBacteria" id="BAD40786">
        <property type="protein sequence ID" value="BAD40786"/>
        <property type="gene ID" value="STH1801"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1801"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1141575_0_0_9"/>
    <dbReference type="OMA" id="DDTLWVN"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="239" mass="27251" checksum="09D91A32E995E01D" modified="2004-10-11" version="1">MGFTGHAFVDWDDTIAENIRYFREVEAQISLLIAGRTGFDRDEVQRRGAELDVAVARQMGLVRESLAVAWLTCYREFAGRAGRPVDPVVEAELRRLCAIPYETRQELLPGAAETLRWLHEAGFEVTIWTAGEDAVQRRKIQESGLTRWIHRQTVVPEKTAERLRDAIGDRDPGHCFVVGNSRHSDIRPALTLGVAAYHVDVETWAYDHLDVDEEDPNYHRLTHIGELPRLLAQRFRLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT5</accession>
    <name>Q67NT5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lar_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195801.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1673"/>
    <dbReference type="EnsemblBacteria" id="BAD40658">
        <property type="protein sequence ID" value="BAD40658"/>
        <property type="gene ID" value="STH1673"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1673"/>
    <dbReference type="eggNOG" id="COG3875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050189_0_0_9"/>
    <dbReference type="OMA" id="SPGGFPK"/>
    <dbReference type="OrthoDB" id="313921at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050043">
        <property type="term" value="F:lactate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043166">
        <property type="entry name" value="LarA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018657">
        <property type="entry name" value="LarA-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09861">
        <property type="entry name" value="Lar_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lar_N" evidence="1">
        <location>
            <begin position="8"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="420" mass="46276" checksum="69B9D3B9D9CCE3C2" modified="2004-10-11" version="1">MKTPLKIGQSEMLLELPDENLKGILLPNEVAPLPNPLKTVEHVLKSPTGTPPLAELLRARRPERVVIIVNDITRPTPYDIMLPPLLRTLDEAGVRPEQVTFVVATGIHRPHTDEENRRIFGADVVNRYRFVNHDCVADDLVTVGRLSDGTDLTVNRHVAEADFLITTGLIGLHYFAGYSGGRKSVLPGVAGKSLITHNHRKMTDPRAATARYKDNPVHHIMMEAARMVGVDFILNVVTNEKKQIVAVVAGDLEAAWLEGVAVCERMSVVRVDEQADVAICSAGGFPKDLDVYQSQKAMEAAEQVTRPGGTIVLVADCREGYGGEAAFERWVREADSLDDILRRFESHFELGGHKAFAIARVLKEKEVVLVSPQPSDLAESLFMRPAATLDEALAYVRRKHGDDFKAYVLPHATLVFPVVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NU0</accession>
    <name>KCY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">STH1668</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NU0"/>
    <dbReference type="SMR" id="Q67NU0"/>
    <dbReference type="STRING" id="292459.STH1668"/>
    <dbReference type="EnsemblBacteria" id="BAD40653">
        <property type="protein sequence ID" value="BAD40653"/>
        <property type="gene ID" value="STH1668"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1668"/>
    <dbReference type="eggNOG" id="COG0283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_0_0_9"/>
    <dbReference type="OMA" id="RAITWWM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000131991" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <sequence length="226" mass="24562" checksum="C56CFD470DC41ED4" modified="2004-10-11" version="1">MTDGTRELVIAMDGPAGAGKSTVARIVANRLGYLYIDTGAMYRALALKALRLGIPETDAAALADLADATEVELQRAPDGGNRVLLDGEDVTAEIRSPAVSAVVSQVSAVPRLRQRLIEVQRSMARAGGVVMDGRDIGSYVLPHADRKFYITASLQERARRRVAQLRAEGHEADLAAVEAEIARRDEQDMNKGVHSLVQLPESIVIDTTGKRVDEVVEEILRHCRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV3</accession>
    <name>Q67NV3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH1655</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1655"/>
    <dbReference type="EnsemblBacteria" id="BAD40640">
        <property type="protein sequence ID" value="BAD40640"/>
        <property type="gene ID" value="STH1655"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1655"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_2_1_9"/>
    <dbReference type="OMA" id="AFILQYM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001516">
        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="546"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="577"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="602"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="628"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="666"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="723"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_N" evidence="11">
        <location>
            <begin position="42"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="10">
        <location>
            <begin position="104"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4040" evidence="12">
        <location>
            <begin position="587"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="754" mass="80903" checksum="FE9C821A3DD375B2" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW8</accession>
    <name>Q67NW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spore germination protein KB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gerKB2</name>
        <name evidence="7" type="ordered locus">STH1640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1640"/>
    <dbReference type="EnsemblBacteria" id="BAD40625">
        <property type="protein sequence ID" value="BAD40625"/>
        <property type="gene ID" value="STH1640"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1640"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047547_1_2_9"/>
    <dbReference type="OMA" id="WIRERQY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004761">
        <property type="entry name" value="Spore_GerAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34975">
        <property type="entry name" value="PTHR34975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03845">
        <property type="entry name" value="Spore_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="371" mass="40323" checksum="67E8AA19EE72AE33" modified="2004-10-11" version="1">MSVGHRVLISHQQLMFLVYTLHFSATELLLPSSLAETGKSGGWMAPLVSFFLSAVPVALMLGLLVRRHPHLGLGALSHHLLGRLPARLLMLLTTLFNVGLTALCLRDMVEAIPVAILPVTPTLAVALPFLLVAGYGAYCGAEVLARLAFFFMVIAVSLFSLVVVTLLRLVRALHLLPLWDQSPLQLVAAAWPTTGWYAESWTFLPLAAMVDRPQYAGRGLIAGALIAAVHLMSCTALSIGIFGHSLVAHFAFPIHALFQQITIGEFVERLDVILITICLLGMIVKTATHLWLAVDAAQFALGLRNQRPLLPALGLAAVLWMLSIPNLPWLFAFSTTVWTPFSLCLGLGVPALLLAASWIRERQYRPPDISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P25</accession>
    <name>Q67P25_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyrazinamidase/nicotinamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195712.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1583"/>
    <dbReference type="EnsemblBacteria" id="BAD40568">
        <property type="protein sequence ID" value="BAD40568"/>
        <property type="gene ID" value="STH1583"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1583"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1204290_0_0_9"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="25359" checksum="49DB1FDBDEF9C1BA" modified="2004-10-11" version="1">MAKRALFVIDTQVDFNDEQGSLTSFRRSDPKMQRIADLMDEVHREGGFVVATADTHPPLHHPEHLVTYRRMLQETMGPDRFEAYMDKAYEAYREELKLYPPHCEYGTPGWQPTAVIAAAFERLGDDLILIQKPTYRLVDGCVMKGPAALVGRTGVQVLEFLKGQGVEEVIVTGLITPVCVLAAVESAVGAGFRVTVDERLVDSYDEDSHRQGLERIAGAGGIVVPAADGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P65</accession>
    <name>Q67P65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheX1</name>
        <name evidence="3" type="ordered locus">STH1543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195673.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1543"/>
    <dbReference type="EnsemblBacteria" id="BAD40528">
        <property type="protein sequence ID" value="BAD40528"/>
        <property type="gene ID" value="STH1543"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1543"/>
    <dbReference type="eggNOG" id="COG1406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116290_1_0_9"/>
    <dbReference type="OMA" id="LYEFPEN"/>
    <dbReference type="OrthoDB" id="1956590at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038756">
        <property type="entry name" value="CheX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028051">
        <property type="entry name" value="CheX-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39452">
        <property type="entry name" value="PTHR39452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13690">
        <property type="entry name" value="CheX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheX" evidence="2">
        <location>
            <begin position="42"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16242" checksum="D936C925C7C59B81" modified="2004-10-11" version="1">MKAEFVNPFLVSAGQVLQTETGTEVVQGEVRLEESPLESDEVTVLIGVVGRVQGLVLYGMSEETGRKLVSAMTGEEVTVFDDMCESAVAELGNVITGLASGELEAAGYPCKIAPPSVVMGKGTAISTLSIKRLVIPLETKLGSITVHVALRETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB2</accession>
    <name>Q67PB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1496"/>
    <dbReference type="EnsemblBacteria" id="BAD40481">
        <property type="protein sequence ID" value="BAD40481"/>
        <property type="gene ID" value="STH1496"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1496"/>
    <dbReference type="eggNOG" id="COG0020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_1_1_9"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="OrthoDB" id="1630604at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="28944" checksum="5D02D915EE7A0D32" modified="2004-10-11" version="1">MTDDLHGRVEPDRAAPLHTAPRGPDYRPRHVAIIMDGNGRWALRRGLPRTVGHCAGVEALRGVVKAAPDLGIKILTCYAFSTENWKRPKDEVDTLMSLLLEYCRLEAENLHRNGVRINAIGRIHELPPAQQQEIARAVALTRGNDRLILNLAVNYGGQAELVDAFRSLAAEVQSGRLAPEAIDAEVVRRHLYTADLPDPDLIIRTAGEMRLSNFLLWQSAYAEIWVTDVYWPDFTREHLEQALRDYARRERRFGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB9</accession>
    <name>Q67PB9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1489"/>
    <dbReference type="EnsemblBacteria" id="BAD40474">
        <property type="protein sequence ID" value="BAD40474"/>
        <property type="gene ID" value="STH1489"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1489"/>
    <dbReference type="HOGENOM" id="CLU_1304355_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="21943" checksum="0C04DDF65E44E70D" modified="2004-10-11" version="1">MSARGPGIPMWVAGVLRASGGRLPADLLRGGMGMAGGVPGVDRRSAPFWRGLLVGAGAVAAALAMGLGYLATAGVTVTVTGDLLVARMGEEVEAAVRRELSGVLRQAQAEWPQRVQEAARSQIAAVRVEVAGLSLELPEAARTQVERAAAEAAQAAVESVAGNLPVDELAERIGARAAALARERLPQVLAEQRIVVEAAPWLRVPVRLQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC0</accession>
    <name>Q67PC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195618.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1488"/>
    <dbReference type="EnsemblBacteria" id="BAD40473">
        <property type="protein sequence ID" value="BAD40473"/>
        <property type="gene ID" value="STH1488"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1488"/>
    <dbReference type="HOGENOM" id="CLU_2235206_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="105" mass="11638" checksum="FBE5FE1EF79C2ED5" modified="2004-10-11" version="1">MAVRPIDSLTMLPRLQEAGRAVQQTEQYPHAFQQVLGAQVQQEGERSQKQVRRRTEAEQPTVTPDGRRPGGQGQPSGRGEHHRRRQARPEPGDAPGGTGRLDVKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD9</accession>
    <name>RIMM_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimM</name>
        <name type="ordered locus">STH1469</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195599.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PD9"/>
    <dbReference type="SMR" id="Q67PD9"/>
    <dbReference type="STRING" id="292459.STH1469"/>
    <dbReference type="PRIDE" id="Q67PD9"/>
    <dbReference type="EnsemblBacteria" id="BAD40454">
        <property type="protein sequence ID" value="BAD40454"/>
        <property type="gene ID" value="STH1469"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1469"/>
    <dbReference type="eggNOG" id="COG0806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077636_3_2_9"/>
    <dbReference type="OMA" id="KFYFHEV"/>
    <dbReference type="OrthoDB" id="1497415at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000163373" description="Ribosome maturation factor RimM">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="PRC barrel" evidence="1">
        <location>
            <begin position="97"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <sequence length="174" mass="19565" checksum="2E5AF173C5B47ECF" modified="2004-10-11" version="1">MAGQSRPDLIRIGQVTAPHGVRGAVRVYPTTDFPERFVTLKRVMIDGPDRAVGARFRGFVKNLVILELEGITDRNQAERLRGADLLVPREEVHPLPEGYYYDFDIIGIEVVDPDGRQLGRVVEVDHTSPVHDLYVVETAPGKRYLVPAVRRFVKEIDLETGRMVIDPIPGLLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK3</accession>
    <name>Q67PK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1405"/>
    <dbReference type="EnsemblBacteria" id="BAD40390">
        <property type="protein sequence ID" value="BAD40390"/>
        <property type="gene ID" value="STH1405"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1405"/>
    <dbReference type="HOGENOM" id="CLU_465334_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269599" evidence="2">
        <location>
            <begin position="31"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="586" mass="63334" checksum="ED5B067BD1696EFC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN3</accession>
    <name>Q67PN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195505.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1375"/>
    <dbReference type="EnsemblBacteria" id="BAD40360">
        <property type="protein sequence ID" value="BAD40360"/>
        <property type="gene ID" value="STH1375"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1375"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1208194_0_0_9"/>
    <dbReference type="OMA" id="GRIECIH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="233" mass="25620" checksum="24EF26B53E06A804" modified="2004-10-11" version="1">MSLPLVLVGCPVRNRARVLPAYLSALEAMDYPPGRLRFAFVLNDSVDESEAILRAFAERRPTALLRCDLGFPRWRRGHYSYANLALLRNLLIEEALRSGADYLFSVDSDVLPPPHALRRLLAAARPIVGARVPNDLHLPGEHWRCNFLCADDRGRLRHPRSFPPDALMSVDVVGAAVLIARAVLEAGCRYAPAGTGEDEGFCRQAKAAGFQPWVDTGVVCRHLMQEGDGGPEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP9</accession>
    <name>RL28_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmB</name>
        <name type="ordered locus">STH1359</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195489.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PP9"/>
    <dbReference type="SMR" id="Q67PP9"/>
    <dbReference type="STRING" id="292459.STH1359"/>
    <dbReference type="EnsemblBacteria" id="BAD40344">
        <property type="protein sequence ID" value="BAD40344"/>
        <property type="gene ID" value="STH1359"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1359"/>
    <dbReference type="eggNOG" id="COG0227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064548_7_0_9"/>
    <dbReference type="OMA" id="RINVCTS"/>
    <dbReference type="OrthoDB" id="2062238at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribo_L28/L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribo_L28/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001383">
        <property type="entry name" value="Ribosomal_L28"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00009">
        <property type="entry name" value="L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000178571" description="50S ribosomal protein L28">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00373"/>
        </source>
    </evidence>
    <sequence length="63" mass="7086" checksum="B290E32C311A9178" modified="2004-10-11" version="1">MARRCEVCNKGVSHGHNVSHAENKTKRTWSPNVQTVRAVVDGRVKRITVCTRCLRSGKVQRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS1</accession>
    <name>Q67PS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195468.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1337"/>
    <dbReference type="EnsemblBacteria" id="BAD40322">
        <property type="protein sequence ID" value="BAD40322"/>
        <property type="gene ID" value="STH1337"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1337"/>
    <dbReference type="eggNOG" id="COG1561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076609_1_0_9"/>
    <dbReference type="OMA" id="EIKSVNH"/>
    <dbReference type="OrthoDB" id="1509828at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005229">
        <property type="entry name" value="CHP00255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013551">
        <property type="entry name" value="DUF1732"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013527">
        <property type="entry name" value="YicC-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30636">
        <property type="entry name" value="PTHR30636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08340">
        <property type="entry name" value="DUF1732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03755">
        <property type="entry name" value="YicC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00255">
        <property type="entry name" value="TIGR00255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YicC_N" evidence="1">
        <location>
            <begin position="3"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1732" evidence="2">
        <location>
            <begin position="208"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="292" mass="32507" checksum="F1299BC1486F13BF" modified="2004-10-11" version="1">MARSMTGFGRGEASGEAGRVTVEVKAVNHRFSEVVFRMPRQFSALEDRVRRLIQGRTARGRFEVYVAWEPSAEARAVKVDKDLALAYYNALRQLAEEIGSNQELSLDTLARLPEVLSVQEGELSDDEIWALLEPALTQAMAGLIAMREREGAALAADLAGRLDRLEAFRAAAAARAPQVVEEYRQRLTRRLEELLPPTNPVDPQRLAQEVALFADRADITEELQRLASHIDQFRRALETDDAVGRKLDFLVQEMGREVNTIGSKANDADLAAAVVAAKSELEKIREQVQNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PS9</accession>
    <name>Q67PS9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195460.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1329"/>
    <dbReference type="ESTHER" id="symth-q67ps9">
        <property type="family name" value="UCP033634"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40314">
        <property type="protein sequence ID" value="BAD40314"/>
        <property type="gene ID" value="STH1329"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1329"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111932_0_0_9"/>
    <dbReference type="OMA" id="KDHQYNA"/>
    <dbReference type="OrthoDB" id="1200636at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017018">
        <property type="entry name" value="UCP033634"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033634">
        <property type="entry name" value="UCP033634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="22172" checksum="2B864D8BD7D6E2C6" modified="2004-10-11" version="1">MRMEAVPTPSGAGVTIQNRLAGPEKGSERLLVLLPGNNYSCDAPAFFYLKQAAVQAGWDVLSTAYAFQLTGGEVDGPAMLADVRAALDEVLPRGYREICIAAKSLGSAIALPLARSLAGYRVSLLILTPVPQFLTEPVGDLRTLVVIGTNDPVYHMTECGAARKRADAEWAVIPGLDHGFNVPGDWKASAAALEQVIAPCVRFLAGPAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT6</accession>
    <name>Q67PT6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative arginase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1322"/>
    <dbReference type="EnsemblBacteria" id="BAD40307">
        <property type="protein sequence ID" value="BAD40307"/>
        <property type="gene ID" value="STH1322"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1322"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066809_1_1_9"/>
    <dbReference type="OMA" id="ILMFDEY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="255" mass="27974" checksum="F885ED6A6D5188B6" modified="2004-10-11" version="1">MLNLDDTLTWQPALLAAPHEWIDLRHLPGKNACATPEALAAIAAALAPHQEVPLCFWGAGEYHYVTLLRLQALRRPVTLVLFDHHHDAAPLQGDLVTCGNWVRHALNLPWVRRAVWVGGRHPEMQLYLPHPRLLRAAEPTPPEPFAEWALQAIPTADVYISIDKDVLTGDDSATTWGAGDLALAELMDWLKALARHRRIVGADVCGEWSLSPAQVFPGPQDRRAIARNQRANLAIRDLLAPALVPGAPAAPSRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU1</accession>
    <name>Q67PU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-oxoacid:ferredoxin oxidoreductase alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1317</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195448.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1317"/>
    <dbReference type="EnsemblBacteria" id="BAD40302">
        <property type="protein sequence ID" value="BAD40302"/>
        <property type="gene ID" value="STH1317"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1317"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_1_0_9"/>
    <dbReference type="OMA" id="LNPFPKN"/>
    <dbReference type="OrthoDB" id="360732at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022367">
        <property type="entry name" value="2-oxoacid/accept_OxRdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03710">
        <property type="entry name" value="OAFO_sf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="14"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="POR_N" evidence="3">
        <location>
            <begin position="208"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="4">
        <location>
            <begin position="476"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="609" mass="64878" checksum="B083545406EF747B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q47</accession>
    <name>MURB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylenolpyruvoylglucosamine reductase 2</fullName>
            <ecNumber evidence="1">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramate dehydrogenase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murB2</name>
        <name type="ordered locus">STH1211</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurB family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q47"/>
    <dbReference type="SMR" id="Q67Q47"/>
    <dbReference type="STRING" id="292459.STH1211"/>
    <dbReference type="EnsemblBacteria" id="BAD40196">
        <property type="protein sequence ID" value="BAD40196"/>
        <property type="gene ID" value="STH1211"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1211"/>
    <dbReference type="eggNOG" id="COG0812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035304_1_1_9"/>
    <dbReference type="OMA" id="KMNAGMK"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224729" description="UDP-N-acetylenolpyruvoylglucosamine reductase 2">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="31"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <sequence length="308" mass="33219" checksum="AAF85F733C1F4EC2" modified="2004-10-11" version="1">MSPMAERLRSIIRDPGRIRRAEPLKRHTSVRIGGPADYLVEVADRHELSRLLRLAGEEALPVYILGSGSNLVVSDEGVRGLVLRLTGEFARIAVDGSTVRVGGGCSLPKLAHQASRRGLGGLEFACAIPGTVGAGLVMNAGAHGGDMAQVVAEATVIWGDGRMERLCPGEIGFAYRSTRLQGTSAIVAEVVMALRPADRAALEGAMRQHLNRRRATQPLQYPNAGSVFKNPPGDYAGRLIEQAGLKGERVGDAQVSEKHANFIVNLGQATARDVLTLMDRVRSTVERRFGVRLEAEVKIWGHNPWFPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI6</accession>
    <name>Q67QI6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin reductase</fullName>
            <ecNumber evidence="4">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1072</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195204.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1072"/>
    <dbReference type="EnsemblBacteria" id="BAD40057">
        <property type="protein sequence ID" value="BAD40057"/>
        <property type="gene ID" value="STH1072"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1072"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_9"/>
    <dbReference type="OMA" id="FRAEDYW"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="6">
        <location>
            <begin position="3"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="314" mass="34072" checksum="1E855B6B1D2A820F" modified="2004-10-11" version="1">MRKVVILGTGPAGLTAAIYTARANLQPLVIEGNEPGGQLTTTTEVENFPGFPDGIMGPELMDNMRRQAERFGAEFRRGYVTGVDFSRRPFRITVDETDVIEAEAVIIATGASAKHLGIPGEDENIGRGVSYCATCDGFFYRGKKVLVVGGGDSAMEEANFLTKFADVTVVHRRSELRASKIMQERARANPKISWVLNAVPVEVLADERGFKALKVRDKETGEERLLEADGMFVAIGHKPNTDFLRGQIDMDELGYIKTIPGTAQTNVPGVFACGDVQDSRYRQAITAAGSGCMAALDCERFLEEVAAQEGSLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI8</accession>
    <name>Q67QI8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1070"/>
    <dbReference type="EnsemblBacteria" id="BAD40055">
        <property type="protein sequence ID" value="BAD40055"/>
        <property type="gene ID" value="STH1070"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1070"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_34_6_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017255">
        <property type="entry name" value="AcTrfase_GNAT_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037663">
        <property type="entry name" value="Acetyltransf_GNAT_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="15669" checksum="23192E0991C3C50B" modified="2004-10-11" version="1">MEMEESLVIRPATAADAPAVAALLTDFNGEPTDAAASARRMAAMEGTETVLLALRGGAAVGLCSLRVVPFLSADHPYAEVTELYVAPQHRRQGIARRLMAEAEALARAKGAPAVYLLTGFRNETAQAFYRSIGYGDYALAMRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK2</accession>
    <name>Q67QK2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2007 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195188.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1056"/>
    <dbReference type="EnsemblBacteria" id="BAD40041">
        <property type="protein sequence ID" value="BAD40041"/>
        <property type="gene ID" value="STH1056"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1056"/>
    <dbReference type="HOGENOM" id="CLU_2425876_0_0_9"/>
    <dbReference type="OrthoDB" id="1912367at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018551">
        <property type="entry name" value="DUF2007"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09413">
        <property type="entry name" value="DUF2007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2007" evidence="2">
        <location>
            <begin position="5"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="9711" checksum="29DCFBFB8CE252FC" modified="2004-10-11" version="1">MNYEWVEVYKAPDHVEAELVRGLLEASGIPVVTEARGAQSLPFLLGPARVGGHISIRVPPEHAQAAREVLAAREEPSWPSGPGGGNGEDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM5</accession>
    <name>Q67QM5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Subtilisin-like alkaline serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1033</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195165.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1033"/>
    <dbReference type="MEROPS" id="S08.026"/>
    <dbReference type="EnsemblBacteria" id="BAD40018">
        <property type="protein sequence ID" value="BAD40018"/>
        <property type="gene ID" value="STH1033"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1033"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_15_5_9"/>
    <dbReference type="OMA" id="GINHAVK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase_S8" evidence="4">
        <location>
            <begin position="36"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="290" mass="31255" checksum="33473DF97589EBAD" modified="2004-10-11" version="1">MGRTIWRTPPWHIRRTGFPEAWRLLRLRRRRRGPVEIGLLDSGVDLTHPYLRDLLGPGINLLSPGDLPHDDHGHGTHVAGILAVASGAVGGQRPVWPPPLRIRPVKIFDRQGYGRIRDIIAGLQWCRAQGCEIVNLSFGTDRRASEELAEAIRALEKAGILLVGAAGNDGRGGAVDTPGKYPEVLAVAAINRRDRLARFSSRGPEVDLVAPGAGLFSLAPGGRFCRMSGTSMAAPHVTAAAAMLLSVRPGLTPAAVRAHLKHTAEWLPQIHASAQGAGLLRADRLLREEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS6</accession>
    <name>Q67QS6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH982</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH982"/>
    <dbReference type="EnsemblBacteria" id="BAD39967">
        <property type="protein sequence ID" value="BAD39967"/>
        <property type="gene ID" value="STH982"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH982"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_2_9"/>
    <dbReference type="OMA" id="FSATQMF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="279" mass="29249" checksum="5DE9A56EC8AEE866" modified="2004-10-11" version="1">MTPVALTLAILLTSVLAGLVGSMLGLGGGIIIVPALTLFFGYDFRTAVAASAVAIIATSTGAAVAYLRDHITNTRIAMFLEIGSTLGALSGAFIAGYVPARFLFLLFAALMAYSAFSMFRARKSELREDVVPDRLSERLQLRGAYYDKALGRTISYRATGALPGLLLMYVSGAFAGLLGVGGGTFKVPAMDIVMKLPIKVSTATSNFMIGVTAAASAAVYFVRGDVQPLVAGPVALGVLCGALLGSRLMVRMKGSTLRLLFVPLLVYVAVQMAWKGVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QW3</accession>
    <name>Q67QW3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39930">
        <property type="protein sequence ID" value="BAD39930"/>
        <property type="gene ID" value="STH945"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH945"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_626884_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="437" mass="46499" checksum="165C6A8AC3FCB856" modified="2004-10-11" version="1">MTVMAGCAPREGGAPRPTGQEPPAVAGQEPQAAQGPARAEVPGGPGAPVREEIPGGFRLGPFDESGSAWWLSPDAVWVYAKVGGAARYGAVSLAGGGLEPGPGHDPEDLPYWDRWHLLLSPDAGRAVVYSFHGFDRTFVDRETGAVSEPQAPNFGGSHMFDSWSPDGSRFLVQSVRTDPVPGFYFLDRDGNPAGTFAEPGYYSHRGVWSPDGKRLAFLSVPADLFYPRIPEEWEEPPYGERVGVLDPETGRVTYFAIDGLTFYAHPVWSPDGERLAVVCGERTTVRLDGPWEGYETTRLRGPRPCLIDPEAGTIAPMGDPAEGDVTIVILGLADEAALVAWARPSDGAGYGWVPLGGGALVDLGGTWSVEGAQLVAGGRAALTGQLLPNKLVLFNQRGEVERILAEGAIQGLTASPDGRHVAFFDGGYLTVLRVPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX6</accession>
    <name>Q67QX6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tautomerase</fullName>
            <ecNumber evidence="3">5.3.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH932</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 4-oxalocrotonate tautomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195064.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH932"/>
    <dbReference type="EnsemblBacteria" id="BAD39917">
        <property type="protein sequence ID" value="BAD39917"/>
        <property type="gene ID" value="STH932"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH932"/>
    <dbReference type="eggNOG" id="COG1942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_1_2_9"/>
    <dbReference type="OMA" id="NWGIGGQ"/>
    <dbReference type="OrthoDB" id="2013680at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018191">
        <property type="entry name" value="Tautomerase_Pseudo-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00013">
        <property type="entry name" value="taut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; via imino nitrogen" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR618191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="67" mass="7514" checksum="7E3C4CEBC42CC46C" modified="2004-10-11" version="1">MPYVNIKITREGATPEQKKQLIAGATRLLQEVLGKNPQTTFVIIEEVDTDNWGIAGETVTERRKKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R08</accession>
    <name>Q67R08_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion-translocating oxidoreductase complex subunit A</fullName>
            <ecNumber evidence="8">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rnf electron transport complex subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnfA</name>
        <name evidence="9" type="ordered locus">STH900</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NqrDE/RnfAE family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195032.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH900"/>
    <dbReference type="EnsemblBacteria" id="BAD39885">
        <property type="protein sequence ID" value="BAD39885"/>
        <property type="gene ID" value="STH900"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH900"/>
    <dbReference type="eggNOG" id="COG4657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095255_1_0_9"/>
    <dbReference type="OMA" id="CPFFGVS"/>
    <dbReference type="OrthoDB" id="1814639at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00459">
        <property type="entry name" value="RsxA_RnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011293">
        <property type="entry name" value="Ion_transpt_RnfA/RsxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003667">
        <property type="entry name" value="NqrDE/RnfAE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02508">
        <property type="entry name" value="Rnf-Nqr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006102">
        <property type="entry name" value="NQR_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01943">
        <property type="entry name" value="rnfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="20791" checksum="A634313B7D35DF60" modified="2004-10-11" version="1">MEILAILIGGILVNNYIFSRFLGLCPFFGVSNKLETVYGMGAAVVFVMTLASAITWPLQHLLLEPLGLGYLQTVAFILVIAALVQTVEMVIQRVSPTLYAALGIFLPLLTTNCAVLGAALLNITQGYGFMTSVLNGFACGVGWTLATLLFAGVRERIEGAHIPPAMRGMPIAFVAAGLMALAFMGFQGLFRGILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R36</accession>
    <name>Q67R36_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195004.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH872"/>
    <dbReference type="EnsemblBacteria" id="BAD39857">
        <property type="protein sequence ID" value="BAD39857"/>
        <property type="gene ID" value="STH872"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH872"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_5_0_9"/>
    <dbReference type="OMA" id="NSMAGQN"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="462" mass="49077" checksum="7E0E085132CB112C" modified="2004-10-11" version="1">MRRGSQRDFTQGSIPRHIIMFSLPLFAGNLLQMLYNVVDSIWVGQVIGKNALGAVSVSQPLIFALISLVVGLTMATTTLVGQYRGAGKEELVQRTIGTSLILLAGLGAVVGVLGVLLRYPLLRMIDTPEEIIHDAAAYLAIFMGGILFLFLYNTLGAILRGLGDANTPLIALAIATVLNIILDGIFILGLGPVPPMGVPGVAIATVVAQGISAIWLARWILKKTDLIRLSRDYWRVDWGLAGRIFRIGLPAGLQQAVVSFGMVTVTALINSYGPDVVAAFGAAQRLDQAAFLPSMSMGLAVTSVVAQNLGAGKLDRVSAAVRWCAGLGVAITGLVTLVAVLKPTILIALFNREPDVLAAGSLYLRINGWSYIFFALVFVLGGVMRGAGDTMATLVLTIIGLWVVRVPAAYLLARSFGMGPAGIWTGILISAIASFLLHLAYYSTGRWKRKVPSVEPAVAERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R82</accession>
    <name>Q67R82_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH826</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH826"/>
    <dbReference type="EnsemblBacteria" id="BAD39811">
        <property type="protein sequence ID" value="BAD39811"/>
        <property type="gene ID" value="STH826"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH826"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_424467_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033463">
        <property type="entry name" value="sCache_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17202">
        <property type="entry name" value="sCache_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="374"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="470"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="791" mass="83435" checksum="FAD123BAD6D793D6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RC3</accession>
    <name>Q67RC3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH785</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194918.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH785"/>
    <dbReference type="EnsemblBacteria" id="BAD39770">
        <property type="protein sequence ID" value="BAD39770"/>
        <property type="gene ID" value="STH785"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH785"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_9"/>
    <dbReference type="OMA" id="ICIDTDE"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="303"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="243"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="431" mass="45899" checksum="312D822C91D6B060" modified="2004-10-11" version="1">MPIAVAGAGYVGLTTAACLCELGHEVALLEIDGERVARLRRGECPFYEPGMDRLLRRHLGRSLTVTTSLDEAIRRAGMIIVCVGTPPSSSGAPDLQALWRLLDDLRVRRPAEPLTVVLKSTVPPGTNRRAQGRLGAGFAVVSNPEFLREGTAIHDFFHPDRIVVGAESAPAARQVADLYRGIDAPLVTTGWEEAELIKYASNAFLAVKLSFANEVAALADALGADGLAVLKGVGLDRRIGGHFLHPGPGYGGSCLPKDLAALLWTARRRRVRLDLLPAARRANLRQRERVLAMLEPAAGRRIAVWGLAFKAGTDDVRESPALAIVPALLAQGARVLAHDPQARGNFAAALAAGQAQGLPAGLELVDDPWEAIRGADALLVLTEWPEYAAAPPEEIRSALAGDLVVDARNLFDPASMAAAGLRYRGVGRRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH9</accession>
    <name>Q67RH9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrite reductase (cytochrome; ammonia-forming)</fullName>
            <ecNumber evidence="2">1.7.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH729</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6 Fe(III)-[cytochrome c] + 2 H2O + NH4(+) = 6 Fe(II)-[cytochrome c] + 8 H(+) + nitrite</text>
            <dbReference type="Rhea" id="RHEA:13089"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome c-552 family.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.2" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194862.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH729"/>
    <dbReference type="EnsemblBacteria" id="BAD39714">
        <property type="protein sequence ID" value="BAD39714"/>
        <property type="gene ID" value="STH729"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH729"/>
    <dbReference type="eggNOG" id="COG3303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035040_1_0_9"/>
    <dbReference type="OMA" id="EMVILWA"/>
    <dbReference type="OrthoDB" id="738283at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042279">
        <property type="term" value="F:nitrite reductase (cytochrome, ammonia-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00548">
        <property type="entry name" value="NrfA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003321">
        <property type="entry name" value="Cyt_c552"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30633">
        <property type="entry name" value="PTHR30633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02335">
        <property type="entry name" value="Cytochrom_C552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000243">
        <property type="entry name" value="Cyt_c552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="SSF48695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269634" description="Nitrite reductase (cytochrome; ammonia-forming)" evidence="9">
        <location>
            <begin position="33"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="48675" checksum="FC0542EAA0744E76" modified="2004-10-11" version="1">MNRKWIIAIGAALAVVLVGVGTFTYVANKTEAVEGPAVSISPNETDPAVFKLLYPAHYDSYMRNGEMHEPALKYASSEKKKSRLDQFPYMRTLWAGMAFSKEYNEARGHLYTLDDVVGGNGTEATQRINDKTNLTCMYCKSAQVPQLIEQMGDAFYNTKLLADNNTDLFVHPISCSDCHDPQTMELRITRPALVEAMERIGRPVENATRQEMRSLVCAQCHVEYFFNPNDANRVYFPWDKGFEPEDMYAYYEEIGFSDWTHAQTGGGMLKAQHPEFEMFQGSVHQRAGLSCADCHMPYVTEGSTKISSHWWTSPFRTFEQSCAQCHRESQEEMAQRVLHTQDRVKEALDRAGIANQDAILAIEKAIAAGVDEEILNQARALHREAQFYWDLASAENSFGFHNPQKFLETLADSIDLARQAELLVTRAMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RK0</accession>
    <name>Q67RK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Branched-chain amino acid ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH708</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194841.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH708"/>
    <dbReference type="EnsemblBacteria" id="BAD39693">
        <property type="protein sequence ID" value="BAD39693"/>
        <property type="gene ID" value="STH708"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH708"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="LVPEHRE"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="29719" checksum="33F797BDB7D8D40A" modified="2004-10-11" version="1">MLVLNNVEVMYERVILVLKGMSLQVPEGKVVALLGANGAGKTTTLKAISGLLKSENGEVTDGKIEFMGERITNLEAAEIVRKGIFQVMEGRHVFEHLTVEENLLAGSYTRTDRRNVRSDLDMVYHYFPKLVHRRRQKAGYLSGGEQQMVAIGRALMARPKLVLLDEPSLGLAPRLVREIFDIIRRLNEETKTTFLVVEQNAMVALSIAHYGYVMENGRIVLEGPVQTLLNNEDVKEFYLGLTGVGRKSYREAKAYKRRKRWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM4</accession>
    <name>Q67RM4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH684"/>
    <dbReference type="EnsemblBacteria" id="BAD39669">
        <property type="protein sequence ID" value="BAD39669"/>
        <property type="gene ID" value="STH684"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH684"/>
    <dbReference type="eggNOG" id="ENOG50338CB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133857_0_0_9"/>
    <dbReference type="OMA" id="FEWYHEL"/>
    <dbReference type="OrthoDB" id="1988870at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="112" mass="13220" checksum="649845941D307F20" modified="2004-10-11" version="1">MEVGGQHGGHGHGHQPWVKHAPLHARPRTLREKVRQFFWGLLFFEWYHELRHERAKYSDVLNLVLFGELLGIPLMNSSIGLRLLPFVLPELDGWKHRQLEEREVIEEVPHVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS3</accession>
    <name>Q67RS3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH635"/>
    <dbReference type="MEROPS" id="S12.950"/>
    <dbReference type="EnsemblBacteria" id="BAD39620">
        <property type="protein sequence ID" value="BAD39620"/>
        <property type="gene ID" value="STH635"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH635"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_1_1_9"/>
    <dbReference type="OMA" id="DDYHAYF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="2">
        <location>
            <begin position="34"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="378" mass="40729" checksum="DEBB411533F71B39" modified="2004-10-11" version="1">MTDGLYETRWPPGLHPAGPAAAGFDPDRLEAAIRLLEQEIAAERLPGAVVVIARDGAVAAHRALGWAAVYPRRRPMTPGTLFDLASLTKVMATLPAVLRLMEMGEIRLDDPLHLFFPEYQGDGRQEIRIRHLLTHTSGLPAGSLSLREPAASQEERIARIAQVPLEAPPGSRIIYSDLGFILLGELVARLSARPLERFVREQVHGPLGLEETGYLPEGERAASAAATEFREHLGRYQCGEVHDQTATALGGVSGHAGLFGTAPEVAAYGQMWLDGGSGVLSPASVAAATRDQTPHIAGDEHRGLGWIVVHEGAAFLSCGDLFSVGSFGHTGFTGTSLWVDPHRRLVVALLTNRVHFGRSDHIRRLRPLFHNAVAAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS9</accession>
    <name>Q67RS9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH629"/>
    <dbReference type="EnsemblBacteria" id="BAD39614">
        <property type="protein sequence ID" value="BAD39614"/>
        <property type="gene ID" value="STH629"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH629"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_739187_0_0_9"/>
    <dbReference type="OMA" id="ETMINKR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031004">
        <property type="entry name" value="rSAM_YfkAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014866">
        <property type="entry name" value="YfkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF2">
        <property type="entry name" value="PTHR42836:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08756">
        <property type="entry name" value="YfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01097">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04478">
        <property type="entry name" value="rSAM_YfkAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="21"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="369" mass="42240" checksum="79BA8133A722D5B3" modified="2004-10-11" version="1">MGEWGFIADPWEPRRGMEDGRFRLTSVEITVTNWCNLRCRHCAVGESLMDRDPERLPLDLILRRLDEVEGLTTLSLTGGELSGSTEVLRRWVAPLLQYAKRRGLQTQVNTNLTFDLGRYEEIAPWVDVFHITWNYRDEAHFHRIVWGHGSREVSPEASRRLFRRIIDNARALAAQGRFVSAETMINAETADHLGAMNRFLAEIGCRRHEVHPMYAVDWASDLPVLPLPAFREAIRRFLAERDPNLWVLFGTFPFLPCSPLPEDQDLLRAVAAAPNVSVRNCPDGRNRVNVDLFTGAVRVTDFGGMEPLGNVRTERLEDAFRRWHAHPRFQRLNCHCPEVGCTGPDLLVAEMYYPEVDFRTRRAVLPTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU5</accession>
    <name>Q67RU5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH613"/>
    <dbReference type="EnsemblBacteria" id="BAD39598">
        <property type="protein sequence ID" value="BAD39598"/>
        <property type="gene ID" value="STH613"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH613"/>
    <dbReference type="eggNOG" id="COG2316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090635_1_1_9"/>
    <dbReference type="OMA" id="EMLGWEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="28"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="22576" checksum="4F22ABE2800D91FE" modified="2004-10-11" version="1">MREEAGSTVTREEAWQLVNEYVKSPRLINHLLAVEACMRYFARLYGEDEETWGLIGLLHDFDYERWPTIPDHPLQGERILAELGVPEDIRYTICSHADALADRYPRRSLRDKVLYAVDELSGLVVATALVRPTKSIHDVDVQAVRKKMKDKRFAAGVSREDILRGVQDLGVDLDEHIGHVIRALQSAADALGLVGTAASDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV4</accession>
    <name>MURQ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetylmuramic acid 6-phosphate etherase</fullName>
            <shortName evidence="1">MurNAc-6-P etherase</shortName>
            <ecNumber evidence="1">4.2.1.126</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetylmuramic acid 6-phosphate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">N-acetylmuramic acid 6-phosphate lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murQ</name>
        <name type="ordered locus">STH604</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-acetyl-D-muramate 6-phosphate = (R)-lactate + N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:26410"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58722"/>
            <dbReference type="EC" id="4.2.1.126"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-sugar metabolism; N-acetylmuramate degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.126" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RV4"/>
    <dbReference type="SMR" id="Q67RV4"/>
    <dbReference type="STRING" id="292459.STH604"/>
    <dbReference type="EnsemblBacteria" id="BAD39589">
        <property type="protein sequence ID" value="BAD39589"/>
        <property type="gene ID" value="STH604"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH604"/>
    <dbReference type="eggNOG" id="COG2103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049049_1_1_9"/>
    <dbReference type="OMA" id="TRNPATM"/>
    <dbReference type="UniPathway" id="UPA00342"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016835">
        <property type="term" value="F:carbon-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046348">
        <property type="term" value="P:amino sugar catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097173">
        <property type="term" value="P:N-acetylmuramic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05007">
        <property type="entry name" value="SIS_Etherase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00068">
        <property type="entry name" value="MurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005488">
        <property type="entry name" value="Etherase_MurQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005486">
        <property type="entry name" value="Glucokinase_regulatory_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040190">
        <property type="entry name" value="MURQ/GCKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10088">
        <property type="entry name" value="PTHR10088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10088:SF5">
        <property type="entry name" value="PTHR10088:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00274">
        <property type="entry name" value="TIGR00274"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01272">
        <property type="entry name" value="GCKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000249668" description="N-acetylmuramic acid 6-phosphate etherase">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="55"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00068"/>
        </source>
    </evidence>
    <sequence length="300" mass="31586" checksum="9E86625464770E05" modified="2004-10-11" version="1">MELNRLVTEGRLQESLEIDQVSTLEMVRIINEQDKQVALAVEKELPHIARAVDLIAERLRAGGRLFYVGAGTSGRLGILDASEIPPTYGAPPDLVQGVIAGGLEAVFRTREGAEDSREQGAADIAARVRPGDVVVGIAASGRTPYTVAALEEARRLGCATVAVTNNPDSALAAAADVAIAPVVGPEVIMGSTRMKAGTAQKMVLNMLSTGAMIRLGKVYSNLMVDMQASNEKLRHRAVRMVVQATGRDEDAAARALEEAGGSVKQAIVALLAGVDARTAREALDRAGGYVREAIRLAQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV8</accession>
    <name>Q67RV8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoesterase, puative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39585">
        <property type="protein sequence ID" value="BAD39585"/>
        <property type="gene ID" value="STH600"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH600"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_0_0_9"/>
    <dbReference type="OMA" id="DTHYGPI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="3">
        <location>
            <begin position="79"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="32810" checksum="971215B951F1DFB7" modified="2004-10-11" version="1">MYLFPALLLVDVARLMYRGARLLAPGVFPPLNLVKVTGAVLLAAYAAVVIYGAWAARSPVVTRYEVTIPKHAGKYRELNVVLVSDTHLGLIIGRGRLERLVADVNRLGPDLVLLAGDIIDDDFRPFQARQMAGVLRGLDAPLGVYSVLGNHDAGSDDLPMFRAAMAEAGIRLLVDEWVAVDDAFYVVGRNDRSHQRASLQDVLHGVDPSRPVLVMDHQPDRLEEAVAAGVDLQVSGHTHRGQVWPGRLITDRIFEVDWGYLRKGNTQFIVSQGWGTWGPPIRVGSRSEIVQITIRFAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA9</accession>
    <name>Q67SA9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH449</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194583.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH449"/>
    <dbReference type="EnsemblBacteria" id="BAD39434">
        <property type="protein sequence ID" value="BAD39434"/>
        <property type="gene ID" value="STH449"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH449"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_18_3_9"/>
    <dbReference type="OMA" id="MYFVRTA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="19014" checksum="11D2E0FD1A883C22" modified="2004-10-11" version="1">MEYQVRHAAPEDAAAVAAVARRTWADTYRGIIPVEIQAAVLPQWYAEERLARAAANPASAFFVAQTGAGEGVGFAQAGPREEPGDAELWRFYVLPEHQRRGLGRRLLQACLGALQAQGPVSRLFVQVEAKNAGGRRAYERLGFHYVREYDDELMGHTTRMCEMCLHLDGQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SE1</accession>
    <name>Q67SE1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH417</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH417"/>
    <dbReference type="EnsemblBacteria" id="BAD39402">
        <property type="protein sequence ID" value="BAD39402"/>
        <property type="gene ID" value="STH417"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH417"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_3_4_9"/>
    <dbReference type="OMA" id="AVIWRVE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="373"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="479" mass="50495" checksum="07D8B27D54A0861E" modified="2004-10-11" version="1">MYDDKFNDPFGRRPEGNPEEPVDQDRTAASEQPARAEEPMTPVEPVAGGASAEQAESRPELELPEIPETPAVTASPPVLYDPPRFEPRPKEKKGGWGRGWRLAAGAVALVVLSAAVGSGSTYYLLKEHLASQPANYQQPAAPGGSGALQPVAQTVAEVGASVIPEIYNRVAPAVVSVYVESYRGFYRSSGTGSGFVVDPAGYILTNYHVVDGAQRITVQFIDGETMTARVVGKDSTSDLAVLKVDPGDRQLVAATLGDSDRVQVGELAIAIGNPYGHAFTVTAGIVSAIGREIVEPTTSIPGAIQTDAAINPGNSGGPLLNSRGEVIGVNTAIEAPSQWSGNVGLGFAVPINTAKEILPTLMAGQTVQRPYLGVYLEDVDQWYARVLGLRTAEGAVVTQVVPGSAAEEAGLRSPQYDRANRLISADVIVALDGEKVTNADDLVKRIQQRKVGDQVELTVARDGQELKVKATLGARPTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH2</accession>
    <name>Q67SH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194520.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH386"/>
    <dbReference type="EnsemblBacteria" id="BAD39371">
        <property type="protein sequence ID" value="BAD39371"/>
        <property type="gene ID" value="STH386"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH386"/>
    <dbReference type="HOGENOM" id="CLU_1554516_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="18333" checksum="BCDF1BD174B92596" modified="2004-10-11" version="1">MTVPAATWNPPAPQHGAVILTRTWRIVVYGDAVPRGARLYVALGPASLAEGYERLFPFLEAQRLCHRHVRTPELLRQLESLAAWAGKSVAVDPPESADPEELAGALDELLAGAGLTGPSLIAGARPFGGRTGLVFIRRAEPRPEQGGARHWLARLLGEGRPGHAPRGLGVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL3</accession>
    <name>Q67SL3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH345"/>
    <dbReference type="EnsemblBacteria" id="BAD39330">
        <property type="protein sequence ID" value="BAD39330"/>
        <property type="gene ID" value="STH345"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH345"/>
    <dbReference type="eggNOG" id="COG0622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_2_1_9"/>
    <dbReference type="OMA" id="ICFGHSH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="4">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="164" mass="17755" checksum="65EA296B15E77E20" modified="2004-10-11" version="1">MLRVGVVSDSHRRFGKVASMRAQCGRLDWLLHAGDHLTDAPRIAEAVGVEPDRVRAVAGNCDFPETEPAELLLELAGVRILLVHGHQHGVKTGPQRLLYRAQEVGARVAVFGHSHIPFLEDVGGVLLLNPGSLSMPRRPQDPPSCAVLELVDGAVRAYHVFLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW1</accession>
    <name>Q67SW1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH247</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194382.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH247"/>
    <dbReference type="EnsemblBacteria" id="BAD39232">
        <property type="protein sequence ID" value="BAD39232"/>
        <property type="gene ID" value="STH247"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH247"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_3_9"/>
    <dbReference type="OMA" id="QRVDMIN"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12918" checksum="0422CE3E48454900" modified="2004-10-11" version="1">MAQYTVPITDATFEAEVLQSELPVLVDFWAPWCGPCRMIGPVLEEYARDNGHRIKIAKLNVDEEQEWAGRFGVMSIPTMILFHKGKPVGQLVGFRPKHEMYQILDSALDQLESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX4</accession>
    <name>Q67SX4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Diaminopimelate epimerase</fullName>
            <shortName evidence="8">DAP epimerase</shortName>
            <ecNumber evidence="4 8">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dapF</name>
        <name evidence="10" type="ordered locus">STH234</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="4 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH234"/>
    <dbReference type="EnsemblBacteria" id="BAD39219">
        <property type="protein sequence ID" value="BAD39219"/>
        <property type="gene ID" value="STH234"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH234"/>
    <dbReference type="eggNOG" id="COG0253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_3_0_9"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="204"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="214"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="8">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="29543" checksum="E709569E2E6A1415" modified="2004-10-11" version="1">MVRFTKMEGAGNDYIYVNNLVEQVPEERLPALARALSDRHFGVGADGLIVILPPAEPGYDFRFRMFNADGSEGEMCGNGMRCFARYCYERGLTARRRMRVQTAAGCVVPEVLVDDAGSFAGVRVDLGPPRILGDRRIAAEGREYPVTVVDTGVPHIVIFVDDVDAVDLAKVGPQLERHPDFPARVNVNFAEAQGPAELRARTWERGSGLTLACGTGASAVLVAACLKGRTGRAARVRMPGGDLQVEWDEATGHVFLTGPAEFVCDGVFLREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX8</accession>
    <name>Q67SX8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Glutamate synthase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">STH230</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH230"/>
    <dbReference type="EnsemblBacteria" id="BAD39215">
        <property type="protein sequence ID" value="BAD39215"/>
        <property type="gene ID" value="STH230"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH230"/>
    <dbReference type="eggNOG" id="COG0067">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0069">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_8_2_9"/>
    <dbReference type="OMA" id="LKTGWDV"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00339"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015930">
        <property type="term" value="F:glutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="12" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="16">
        <location>
            <begin position="22"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD39215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1552" mass="167233" checksum="BCC0A52CA974F1D1" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T06</accession>
    <name>Q67T06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage V sporulation protein AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH202"/>
    <dbReference type="EnsemblBacteria" id="BAD39187">
        <property type="protein sequence ID" value="BAD39187"/>
        <property type="gene ID" value="STH202"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH202"/>
    <dbReference type="eggNOG" id="ENOG5031DDD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112786_0_1_9"/>
    <dbReference type="OMA" id="SKMFLIA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014203">
        <property type="entry name" value="Spore_V_AC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005562">
        <property type="entry name" value="SpoVA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38450">
        <property type="entry name" value="PTHR38450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03862">
        <property type="entry name" value="SpoVAC_SpoVAEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02838">
        <property type="entry name" value="spore_V_AC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="16699" checksum="4F1F7B84C0025152" modified="2004-10-11" version="1">MTIREGASQETQQSLYRRMVARAEPSPPRLRNAVMAFVSGGLVCALGEGITDFWERVLRISHKQAADPTVATLIFLSSLLTGLGYFDKLARHAGAGLAVPVTGFANAMTATALEFKREGLVFGIGGRMFQLAGAVIVYGVVTAFAVGIITGIARLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T44</accession>
    <name>Q67T44_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LigB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH164</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194299.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39149">
        <property type="protein sequence ID" value="BAD39149"/>
        <property type="gene ID" value="STH164"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH164"/>
    <dbReference type="eggNOG" id="COG3384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046582_2_1_9"/>
    <dbReference type="OMA" id="RTEHFAP"/>
    <dbReference type="OrthoDB" id="1728007at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016701">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LigB" evidence="5">
        <location>
            <begin position="4"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="28981" checksum="720414A8F34CD44B" modified="2004-10-11" version="1">MQPAIFAAHGSPMLALEENAYTRALRSLGERYRPRAVIVFTAHWESNIQAISDVAAYETIHDFGGFPPELYRMQYPARGDAATAVRAAELLTEAGIPFVWDRRRGLDHGAWVPLRLIYPDGSVPVVQMSVNPWASPAEQYRIGQALAPLRREDVMILASGGTIHNFATLRWDDPPEADAWAVAFDDWLLGRIREWDLPALFDYERQAPGARLAVPPGGNEHFVPLFYALGAADDQRTVQELHRSYRYGNLSHALWAFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T47</accession>
    <name>Q67T47_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DSBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH161"/>
    <dbReference type="EnsemblBacteria" id="BAD39146">
        <property type="protein sequence ID" value="BAD39146"/>
        <property type="gene ID" value="STH161"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH161"/>
    <dbReference type="eggNOG" id="COG2761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069253_2_0_9"/>
    <dbReference type="OMA" id="KPTNTFD"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="1">
        <location>
            <begin position="7"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="209" mass="23325" checksum="3348AD30B6545BE9" modified="2004-10-11" version="1">MRMAIRLTVFSDFVCPFCYIGEGLVEKLRQEPDLGVEVTWMPFQLNPATPPEGLTLAEYFGGRMPPDRLAMMHAELKARAGAMGLPMDPPPFICNTRRAHELAEFARDRGRLDAVVLPLFQAYFVQGRNLYEERVLGEAAEAAGLDPVEALAAVREGRYADQVDERLALAGRYGIHSVPTFIVNERYKIVGAQPYEVLRDALRSIAQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T97</accession>
    <name>Q67T97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin oxidoreductase iron-sulfur binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194246.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH111"/>
    <dbReference type="EnsemblBacteria" id="BAD39096">
        <property type="protein sequence ID" value="BAD39096"/>
        <property type="gene ID" value="STH111"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH111"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_1_2_9"/>
    <dbReference type="OMA" id="KIDDENC"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="237" mass="25638" checksum="63C6FDA806A58625" modified="2004-10-11" version="1">MTRYAYFVDLSLCAGCEACTVACQALNGLDWNIRYTKVDRMVVGTYPNVKSSFVTTQCLHCDDPPCAAVCPTGATYKTADGPVKIDDENCIGCQYCMTACPYEARTYDAAADVVRKCSFCHDRLGAGERPACEQTCLTGARMVGDLDDPTDPIHQQISAPDVIHIEGTSFYYRLPEHLDRAALPADFKPSVATFAWQSIVQPVGQILMGSTVAALLLSLAVNAFRNRGKEGAQDGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC8</accession>
    <name>Q67TC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">dCMP deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH80</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194215.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH80"/>
    <dbReference type="EnsemblBacteria" id="BAD39065">
        <property type="protein sequence ID" value="BAD39065"/>
        <property type="gene ID" value="STH80"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH80"/>
    <dbReference type="eggNOG" id="COG2131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047993_2_2_9"/>
    <dbReference type="OMA" id="YPCVICA"/>
    <dbReference type="OrthoDB" id="1544522at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="PTHR11086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="15768" checksum="329F786FE6BC7F32" modified="2004-10-11" version="1">MARPSWDEYFMELAQVVAKRSTCNRRKVGTVLVRDKRILTTGYNGSPSGLPHCTDVGCLVVDGHCVRAIHAEQNAIIQAALHGITLKGATCYVTSSPCVHCAKMLIAAGIERIVYMDEYTEQIGLEMARQAGVVMERFTPSSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TH3</accession>
    <name>Q67TH3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-lysine 2,3-aminomutase</fullName>
            <ecNumber evidence="5">5.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH35</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysine = (3S)-3,6-diaminohexanoate</text>
            <dbReference type="Rhea" id="RHEA:19177"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57434"/>
            <dbReference type="EC" id="5.4.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily. KamA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.2" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH35"/>
    <dbReference type="EnsemblBacteria" id="BAD39020">
        <property type="protein sequence ID" value="BAD39020"/>
        <property type="gene ID" value="STH35"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH35"/>
    <dbReference type="eggNOG" id="COG1509">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032161_0_0_9"/>
    <dbReference type="OMA" id="PIWLNTH"/>
    <dbReference type="OrthoDB" id="557227at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050066">
        <property type="term" value="F:lysine 2,3-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025895">
        <property type="entry name" value="LAM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022459">
        <property type="entry name" value="Lysine_aminomutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="PTHR30538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12544">
        <property type="entry name" value="LAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004911">
        <property type="entry name" value="DUF160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00283">
        <property type="entry name" value="L-lysine_2_3-aminomutase_(LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03820">
        <property type="entry name" value="lys_2_3_AblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00238">
        <property type="entry name" value="TIGR00238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="118"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR603739-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD39020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="448" mass="50643" checksum="7313A0121E6C1A12" modified="2004-10-11" version="1">MRMLTKPRDWREIPLWADATEEQWNDWRWQVSHRITNLEQLKQVVNLTEEEEAAIRDSQHLFRLGITPHYATLIDPDDPHCPMRLQAVPKYAELAWADYEMGDPLHEDVDSPVPGITHRYPDRVLFLITHECSLYCRHCTRRRIVGDQEAMSTAMLDQAIAYIRAHPEIRDVLISGGDPLAVPDRRLEYVIKKLRAIPHVEIIRIGTRMPVVLPQRITPELVNMLRQYHPIWLNTHFNHPFEVQHPKAREAMERLADAGIPTGNQSVLLKGVNDCAVVMRRLVHELVKVRCRPYYIYNCDLSEGLSHFRTSVAKGVAIIEALRGHTSGFCVPTFVVDAPGGGGKIPVMPQYLISQSDGRVILRNFEGKISIYHEGTPEDHVADGKCTLCGTDHSEIKIGPAAELWAVRRRAGEVELPEGVTRLGEQKPLITPDQLAALEPAGSGSGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J49</accession>
    <name>Q67J49_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197431.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3320"/>
    <dbReference type="EnsemblBacteria" id="BAD42301">
        <property type="protein sequence ID" value="BAD42301"/>
        <property type="gene ID" value="STH3320"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3320"/>
    <dbReference type="eggNOG" id="COG0629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078758_6_2_9"/>
    <dbReference type="OMA" id="IPVIAWR"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15308" checksum="5913CDB3A4248293" modified="2004-10-11" version="1">MLNSVVLIGRLTKDPELRYTPSGKAVATLRLAVDRGTVNQQGERETDFIDIVVWEKQAETVANYLQKGRLVAVQGRLQIRQYTTQDGQKREKAEVVATTVRFLDSARDHSGGGGGFSGPRREDGMGSELTLGDDEDVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J90</accession>
    <name>Q67J90_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 14">Phosphoenolpyruvate-protein phosphotransferase</fullName>
            <ecNumber evidence="6 14">2.7.3.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">Phosphotransferase system, enzyme I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">STH3279</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 14">General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="2.7.3.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 14 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.9" evidence="6 14"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3279"/>
    <dbReference type="EnsemblBacteria" id="BAD42260">
        <property type="protein sequence ID" value="BAD42260"/>
        <property type="gene ID" value="STH3279"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3279"/>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_7_0_9"/>
    <dbReference type="OMA" id="CNAEWAL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008965">
        <property type="term" value="F:phosphoenolpyruvate-protein phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024692">
        <property type="entry name" value="PTS_EI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006318">
        <property type="entry name" value="PTS_EI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008731">
        <property type="entry name" value="PTS_EIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036618">
        <property type="entry name" value="PtsI_HPr-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05524">
        <property type="entry name" value="PEP-utilisers_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000732">
        <property type="entry name" value="PTS_enzyme_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47831">
        <property type="entry name" value="SSF47831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01417">
        <property type="entry name" value="PTS_I_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="22" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="14 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PEP-utilisers_N" evidence="21">
        <location>
            <begin position="12"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="19">
        <location>
            <begin position="153"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="20">
        <location>
            <begin position="255"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="PEP binding" evidence="16">
        <location>
            <begin position="454"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="15">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF05524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BAD42260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="573" mass="61574" checksum="3682BDC624548989" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC2</accession>
    <name>ISPE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="1">CMK</shortName>
            <ecNumber evidence="1">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispE</name>
        <name type="ordered locus">STH3246</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JC2"/>
    <dbReference type="SMR" id="Q67JC2"/>
    <dbReference type="STRING" id="292459.STH3246"/>
    <dbReference type="EnsemblBacteria" id="BAD42228">
        <property type="protein sequence ID" value="BAD42228"/>
        <property type="gene ID" value="STH3246"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3246"/>
    <dbReference type="eggNOG" id="COG1947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053057_1_1_9"/>
    <dbReference type="OMA" id="RWPSPAK"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000235139" description="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <sequence length="282" mass="30064" checksum="026B9786A61498D7" modified="2004-10-11" version="1">MEIECHAKVNLTLDVLGRRPDGYHEIRSVMVPVSLHDRLVLEPAEEEIVLESRPPATDRLEENLAYRAAALLREATGCSRGAVIRLQKTIPVAAGLAGGSTDAAGVLTGLNRLWGTGLSDGELADLAIRLGSDVPFFIWSRPARVEGIGERVTPLPVAGPLWMVVATPDVPKSTGQVYRWFDELADVGPRPDAGAMEAALARGDAAAVGRALCNVFEQVMLPRHPEIARLKEAMLAAGALGAVMSGAGPSVLGVVPDREAGGRLLERIRPLSRDAWVVRTLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-02" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC8</accession>
    <name>GLMU_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="1">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmU</name>
        <name type="ordered locus">STH3240</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="1"/>
    <dbReference type="EC" id="2.3.1.157" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197353.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JC8"/>
    <dbReference type="SMR" id="Q67JC8"/>
    <dbReference type="STRING" id="292459.STH3240"/>
    <dbReference type="EnsemblBacteria" id="BAD42222">
        <property type="protein sequence ID" value="BAD42222"/>
        <property type="gene ID" value="STH3240"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3240"/>
    <dbReference type="eggNOG" id="COG1207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_15_2_9"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="OrthoDB" id="1381953at2"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00533"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03353">
        <property type="entry name" value="LbH_GlmU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01631">
        <property type="entry name" value="GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005882">
        <property type="entry name" value="Bifunctional_GlmU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038009">
        <property type="entry name" value="GlmU_C_LbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01173">
        <property type="entry name" value="glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000233861" description="Bifunctional protein GlmU">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyrophosphorylase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="254"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="388"/>
            <end position="389"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01631"/>
        </source>
    </evidence>
    <sequence length="471" mass="50885" checksum="B7BB1A233B9C191C" modified="2004-10-11" version="1">MSDITAVLLAAGHGTRMKSDLIKVMHPLAGKPMIGHIVDNVRRAGLEDIVVVVGYQQERIREYLGDRVRYAVQSEQLGTGHAVLQAAGLIDETEGGHVLVMYGDNPFIGPELIERLIRAHVEADAAASLLTAELADPGALGRILRDPATGAFLGSVEYKDATPEQRRIREIWTGVAVFRRAGFTALLNRLDRNNAQGEYYLPQVWEILLQRGEKVQALLLASEEDALAPNDRVELARAEARLRRQINERHMRNGVTIINPDATYIDEDVEIGRDTVIWPFTFIHGKTVIGPHCKIGPMTTIVSSTVAEGCVVEQSVVEESYVGPGCRIGPMAHLRPGCELEGAAEIGNYAELKKAKVGRGVKCHHHSYLGDATIGAGANIGAGTITANYNGVEKFRTEIGSGAFIGTNVNLIAPITVGDGALIAAGSTVGPRLEIPADALVVERAQAVIKEGRAASLKEAWRQRKMNREGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF4</accession>
    <name>Q67JF4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3214</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197327.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3214"/>
    <dbReference type="EnsemblBacteria" id="BAD42196">
        <property type="protein sequence ID" value="BAD42196"/>
        <property type="gene ID" value="STH3214"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3214"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_3_9"/>
    <dbReference type="OMA" id="YGQDDAH"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="8"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="134"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="134"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="236" mass="26992" checksum="6A7B3085F385FF2D" modified="2004-10-11" version="1">MTEGRRFRILIVEDDEKIASILADEFVRYGYDVVLARDYANIKGEFVAARPDVVLMDINLPRYDGYYWCRQIRTVSRVPILFISARSDQMDQVRAIDHGADDYITKPFNLELARAKVKAAIRRAYGEYAAAGEADLLRVGPLVLDRATNRVSCGDRTVELSPKEFRLLWCLARRAGEIVGRETLLEELWDDTEFVDDNTLTVNVTRVRRRLEELGLTGVIETKRGQGYRLNAGWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ1</accession>
    <name>Q67JJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3177"/>
    <dbReference type="EnsemblBacteria" id="BAD42159">
        <property type="protein sequence ID" value="BAD42159"/>
        <property type="gene ID" value="STH3177"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3177"/>
    <dbReference type="HOGENOM" id="CLU_1069304_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="28255" checksum="D91F23B075AD613E" modified="2004-10-11" version="1">MHARIPAAGYLRPLFWSLALRIRGGDGLFDKLRFSVPGGTEHLIPFAHLSRMQEAVELLGAEDFPLLMRLGAVDGLRLQPVRAGVLHDEALRASQRLVAHQVPTLTFHSPSGAALGSLFGGQGEADVAASDSARVSLTPRGIRIALRQFPPPVGFRSTPGLERGWFACFFASLRFGEDGICGLRTPEMGGSGAPVLLPELPKFPPVTRWHRAFVAGRPDVAEVRFAFTPAQDVFRDVLHALTAATQESLRLKRALEIELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ7</accession>
    <name>Q67JJ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197284.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3171"/>
    <dbReference type="EnsemblBacteria" id="BAD42153">
        <property type="protein sequence ID" value="BAD42153"/>
        <property type="gene ID" value="STH3171"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3171"/>
    <dbReference type="eggNOG" id="COG0724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_8_9"/>
    <dbReference type="OMA" id="LPWNTTA"/>
    <dbReference type="OrthoDB" id="2029304at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="95" mass="10382" checksum="B2DC0C9101D601ED" modified="2004-10-11" version="1">MAKTLYFGNLPWAATSEDLHNAVSQYCEVISARIVTDRETGRSRGFGFVEVPDEAAQLVIDALNGAEWAGRPLTVNEARPRDGRANLGLPKKVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JL0</accession>
    <name>Q67JL0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="10">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">STH3158</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:54452"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23624"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3158"/>
    <dbReference type="EnsemblBacteria" id="BAD42140">
        <property type="protein sequence ID" value="BAD42140"/>
        <property type="gene ID" value="STH3158"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3158"/>
    <dbReference type="eggNOG" id="COG0042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_0_3_9"/>
    <dbReference type="OMA" id="RPWLFAD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024036">
        <property type="entry name" value="tRNA-dHydroUridine_Synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004652">
        <property type="entry name" value="tRNA_dU_NifR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00737">
        <property type="entry name" value="nifR3_yhdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dus" evidence="12">
        <location>
            <begin position="25"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD42140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="37747" checksum="A9D1C563ABA9D6CE" modified="2004-10-11" version="1">MKGLSEPLLKPIRLGGLTIERPLALAPMAGVTNWPFRRLCKEHGCGLVVTEFVSDKALLYDSKRTREMIRLLPDERPAGVQLFGADPDTMARAAARVVELEQPDLIDINMGCPAPKVTKGRGGSSLLKEPELAQEIVRRVVQAVAPTPVTVKMRIGWDSRSINAVEVAKRVEEAGARMITVHGRTKEQHYSGRADWGVIAAVARAVSIPVLGNGDIADPREAEARLRTSGVAGLAVGRGALGNPWIFSRTLHYLETGELLPEPGVRERVETALRHLDLMIEYKGEFLAVREMRKHAAWYLKGLHGSAGVRAAVNEAERADDLRALLLGYLDRMEAVAAENVRLLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JV8</accession>
    <name>RL6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplF</name>
        <name type="ordered locus">STH3060</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197175.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JV8"/>
    <dbReference type="SMR" id="Q67JV8"/>
    <dbReference type="STRING" id="292459.STH3060"/>
    <dbReference type="EnsemblBacteria" id="BAD42042">
        <property type="protein sequence ID" value="BAD42042"/>
        <property type="gene ID" value="STH3060"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3060"/>
    <dbReference type="eggNOG" id="COG0097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_1_2_9"/>
    <dbReference type="OMA" id="KPDPYKG"/>
    <dbReference type="OrthoDB" id="1398618at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_L6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000260958" description="50S ribosomal protein L6">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <sequence length="178" mass="19042" checksum="B7AC4688C6A25D8C" modified="2004-10-11" version="1">MSRIGKAPIEIPAGVEVTVEGNLVRVKGPKGELSREIHPAMSITIENGALRVSRPSDEREHKALHGLTRTLIANMVEGVTKGYSKTLELVGTGYRAAKTGNQLTLSVGYSHPVVFNPPPGIEFQVPAPNQVIVAGIDKQLVGQVAADIRATRPPEPYLGKGIKYAGEHIRRKVGKAGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JW0</accession>
    <name>RS5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsE</name>
        <name type="ordered locus">STH3058</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JW0"/>
    <dbReference type="SMR" id="Q67JW0"/>
    <dbReference type="STRING" id="292459.STH3058"/>
    <dbReference type="EnsemblBacteria" id="BAD42040">
        <property type="protein sequence ID" value="BAD42040"/>
        <property type="gene ID" value="STH3058"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3058"/>
    <dbReference type="eggNOG" id="COG0098">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_2_2_9"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="1505038at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131614" description="30S ribosomal protein S5">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <sequence length="166" mass="17204" checksum="2D3137567DB0D481" modified="2004-10-11" version="1">MARNENVQGELKEKMVDLRRVAKTVKGGRRMSFSAVVVVGDGNGRVGAGLGKAAEIPEAVRKGTEDAKKHMIKIPLNGTTIPHEVIGRFGAGKVLLKPASPGTGVIAGGAVRAVLEAAGVRDILTKSLGSNNAANQVLATLAGLRQLKSAEEVAKLRGKPVEQILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JY6</accession>
    <name>Q67JY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197146.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3031"/>
    <dbReference type="EnsemblBacteria" id="BAD42014">
        <property type="protein sequence ID" value="BAD42014"/>
        <property type="gene ID" value="STH3031"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3031"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="VFELKHY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="115"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="20351" checksum="B82B81BDCDB1F3BF" modified="2004-10-11" version="1">MERDASIIQLCQQGHRAGFSRLVEAYQERVYRTAYAFVQHREDAQDLTQEVFLRTVRAIDSLDAGRPLWPWLRRVTTNLALSHLKRRRGHLSLDDLPEAALLVAHGTEPAWTMLDLERALADLPPLWRMALVLRHQEGLAYEEIARMLDLPVGTVKTYLFRARRALRARLETEGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ2</accession>
    <name>Q67JZ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197140.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3025"/>
    <dbReference type="EnsemblBacteria" id="BAD42008">
        <property type="protein sequence ID" value="BAD42008"/>
        <property type="gene ID" value="STH3025"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3025"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_3_2_9"/>
    <dbReference type="OMA" id="GPPGYEI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="15645" checksum="E9B2B7EE38BAD3AE" modified="2004-10-11" version="1">MQRLEGPGVAFVPLLLRIPLAAVFLFAGWPKLTNLAGAAALFGNFGLPGWLGQFAAVLEVVGGLLLLLGLGTRVMGLLFLMEMVVATALVNWGQAWAGGTFDYTAVRMQITAMFACLALVLTGGGAASLDALWLRRRSQGAADGAVIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K28</accession>
    <name>Q67K28_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flagellar biosynthesis protein FlhA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">flhA</name>
        <name evidence="8" type="ordered locus">STH2987</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FHIPEP (flagella/HR/invasion proteins export pore) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2987"/>
    <dbReference type="EnsemblBacteria" id="BAD41970">
        <property type="protein sequence ID" value="BAD41970"/>
        <property type="gene ID" value="STH2987"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2987"/>
    <dbReference type="eggNOG" id="COG1298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015346_3_0_9"/>
    <dbReference type="OMA" id="MAINPGQ"/>
    <dbReference type="OrthoDB" id="436367at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042194">
        <property type="entry name" value="FHIPEP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042193">
        <property type="entry name" value="FHIPEP_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042196">
        <property type="entry name" value="FHIPEP_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025505">
        <property type="entry name" value="FHIPEP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006301">
        <property type="entry name" value="FlhA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001712">
        <property type="entry name" value="T3SS_FHIPEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30161">
        <property type="entry name" value="PTHR30161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00771">
        <property type="entry name" value="FHIPEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005419">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00949">
        <property type="entry name" value="TYPE3IMAPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01398">
        <property type="entry name" value="FlhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00994">
        <property type="entry name" value="FHIPEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="683" mass="73350" checksum="8D3DC777AA2FE944" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K98</accession>
    <name>Q67K98_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type-4 uracil-DNA glycosylase</fullName>
            <ecNumber evidence="3">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2917</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. Type 4 (UDGa) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197034.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2917"/>
    <dbReference type="EnsemblBacteria" id="BAD41900">
        <property type="protein sequence ID" value="BAD41900"/>
        <property type="gene ID" value="STH2917"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2917"/>
    <dbReference type="eggNOG" id="COG1573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044815_1_3_9"/>
    <dbReference type="OMA" id="RPWLIAE"/>
    <dbReference type="OrthoDB" id="1529383at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005273">
        <property type="entry name" value="Ura-DNA_glyco_family4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00758">
        <property type="entry name" value="UDG_fam4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="9">
        <location>
            <begin position="40"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="23168" checksum="39F4771F9900264C" modified="2004-10-11" version="1">MIYTGEPTELDRIPTMEALAEVVKGCQRCGLRAGCTQVVFGEGVWPAGLMCIGEGPGADEDRLGRPFVGRAGQLLDRILEVSGFDRKRNCYIANIVKCRPPGNRAPEPAERQACWPYLRAQIRLVRPKIIVLLGATALQGIIDPSARITRMRGQWIEREGIHFMPTYHPAALLRDPSKKKDVWNDMKQVIARYRELVDPNHYSPYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA9</accession>
    <name>Q67KA9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="6 10">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH2906</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="function">
        <text evidence="3">May be involved in the biosynthesis of molybdopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="6 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197023.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2906"/>
    <dbReference type="EnsemblBacteria" id="BAD41889">
        <property type="protein sequence ID" value="BAD41889"/>
        <property type="gene ID" value="STH2906"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2906"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_9"/>
    <dbReference type="OMA" id="GKPLMHG"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9 10" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="11">
        <location>
            <begin position="186"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD41889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="410" mass="43326" checksum="85C2117F5BB21B0D" modified="2004-10-11" version="1">MDFFTLKSVEEARVEFLGAVTRRMPAERVAREEALGRITAAPVPAPEDLPPFPRSSVDGYAVLSADTFGASEGLPAYLTVTAEIPMGQVSAVPLHPGEAQKIATGGALPEGADAVVMVEHTEQSAPDEIAVLRPVSPGENVMQRGEDCRAGEPLLPAGRMIRAQEAALLAHAGVREVDVVRRPRVAIISTGDELVPPEEAPGPGQIRESNGQAMRALVLRDGGEPHYLGLARDDEQEILSLLRQGLAYDLVLISGGSSVGARDLTSRLIDQLGSPGVLVHGVQLKPGKPTILAVCDGVPVVGLPGHPVSAQVVYGLFVAPLLRQMQGLPPEPEFRPTVRARLTKNVASAAGRTDVVRVRLFRKDGEVWAEPVHGKSGLISTLVRADGFLIIPSAREGLLAGDTVEVEVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC6</accession>
    <name>Q67KC6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2889"/>
    <dbReference type="EnsemblBacteria" id="BAD41872">
        <property type="protein sequence ID" value="BAD41872"/>
        <property type="gene ID" value="STH2889"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2889"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_16_9"/>
    <dbReference type="OMA" id="IYVESKM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038733">
        <property type="entry name" value="Predicted_DNA_bind_prot_RHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12651">
        <property type="entry name" value="RHH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="39"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="219" mass="24153" checksum="DDF495ED36CE96B7" modified="2004-10-11" version="1">MYIYHSLYVGVCEMVDALSGILTRQPFWEAIRKALTEEETVSLASLDLDGFEEINAQAGRETGDKLIALLGGMLRERADSLGWTVGRIGGDEFAILMPGTGLEKAFLQMETLRADFAASAAQAYPELNPTISIGVANFPRDAKDLGGLTQQAGQALYQAKEAGRNSVALPSREEMVLRSCYYTTSQVARLKKLAEQLKKKESVLLREALDDLLRKYDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI9</accession>
    <name>Q67KI9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2824</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2824"/>
    <dbReference type="EnsemblBacteria" id="BAD41809">
        <property type="protein sequence ID" value="BAD41809"/>
        <property type="gene ID" value="STH2824"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2824"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OMA" id="PYHTIGK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="14"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="339" mass="37516" checksum="ED90C939B22D2694" modified="2004-10-11" version="1">MKEKRSEPVSEKLVEIRNLQTHYFTEDGVVKAVDGVDLYINRGETLGVVGESGCGKSVTSLSIMRLIPNPPGKIVGGEILFEGEDLLKKSEAEMRKIRGNEISMIFQEPMTSLNPVYTIGDQIAEAIELHQGLSHKEAIEKAIEMLRLVGIPLPEQRVKEYPHQLSGGMRQRVMIAMALSCNPKLLIADEPTTALDVTIQAQILELMKKLKKELGMAIMLITHDLGVVAEMCERVVVMYAGKVVEEADAVALFQNPLHPYTEGLLKSIPRLDQEVEKLHVIEGAVPNPLNLPEGCRFHPRCPVATDRCRAEQPTLQQVAPGRKVACFLAEERLMKGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KK6</accession>
    <name>Q67KK6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2807"/>
    <dbReference type="EnsemblBacteria" id="BAD41792">
        <property type="protein sequence ID" value="BAD41792"/>
        <property type="gene ID" value="STH2807"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2807"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090569_1_0_9"/>
    <dbReference type="OMA" id="MEVHEEN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="71"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="164" mass="18716" checksum="E8D6532AF2D39732" modified="2004-10-11" version="1">MGVYGQPAYPYPWYMPPHYWWPGSPPRVPQPQPALRDRGRTPYVVNIAQAARQNRNFRTALWTGEHFQVTLMSIPVGEDIGLEVHPTTDQFVRIEEGQGLVQMGPTRDRLEFRQMAYPGSAVMIPAGTWHNITNTGNRPLKVYVIYAPPEHPFGTIHPTKPEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR3</accession>
    <name>Q67KR3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BcrAD_BadFG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196868.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2749"/>
    <dbReference type="EnsemblBacteria" id="BAD41734">
        <property type="protein sequence ID" value="BAD41734"/>
        <property type="gene ID" value="STH2749"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2749"/>
    <dbReference type="eggNOG" id="COG2971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016274_1_1_9"/>
    <dbReference type="OMA" id="PANIMRD"/>
    <dbReference type="OrthoDB" id="1589192at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="1">
        <location>
            <begin position="7"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="33042" checksum="5DAFBA37A0B4758D" modified="2004-10-11" version="1">MTYYLAVDGGGTKCLAVLVHPDQGIVGSGRSGGSNPQSVGRERAVQALTEAVRLACRHLPEGSRIGTAAFGLAGVDTPATEEDARQLAQTALAAAGVSAECVLVENDGLIALRGAAEGGRGLLVVAGTGSVVYAGDGRRFVRAGGWGHRVGDVGSAFHIAQLGLAAAFRSLDAGDSDTPLIRHLCAAVGVDDLYALYDWLYLPGTGVDAIAGLARAVDAAAQAGDRAAAEVLARAGSELAEAAALAARRAGLVDGSAFPVFLVGGVLQNSAAVRAALLQRLAGKAPGGVVEVPRFAPIYGAVIRALGGPAAVDQRLRERLLAADVLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT0</accession>
    <name>Q67KT0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196851.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41717">
        <property type="protein sequence ID" value="BAD41717"/>
        <property type="gene ID" value="STH2732"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2732"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="RINAYLM"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49968" checksum="2DCF7D3030A38EBD" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRRLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWKQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-24" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L13</accession>
    <name>Y2648_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0756 membrane protein STH2648</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">STH2648</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0756 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196770.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67L13"/>
    <dbReference type="STRING" id="292459.STH2648"/>
    <dbReference type="EnsemblBacteria" id="BAD41633">
        <property type="protein sequence ID" value="BAD41633"/>
        <property type="gene ID" value="STH2648"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2648"/>
    <dbReference type="eggNOG" id="COG2707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125889_1_0_9"/>
    <dbReference type="OMA" id="FLEVIYW"/>
    <dbReference type="OrthoDB" id="1858774at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01874">
        <property type="entry name" value="UPF0756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007382">
        <property type="entry name" value="UPF0756_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38452">
        <property type="entry name" value="PTHR38452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04284">
        <property type="entry name" value="DUF441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000388940" description="UPF0756 membrane protein STH2648">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01874"/>
        </source>
    </evidence>
    <sequence length="150" mass="15534" checksum="550520C4CDCB14EE" modified="2004-10-11" version="1">MPGDQVILLSLMALGVVARNALIVTAAGVVLILRVLALERFFPLLERRGLEAGLIFLLIAVLVPFATGEVGWAEIRQSFTSWTGLAAILGGIIAAVLSGYGVTLLQVKPEVIVGMVVGTILGVVLFKGIPVGPLAAAGFTAILLALVRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN4</accession>
    <name>Q67LN4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MarR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196550.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2427"/>
    <dbReference type="EnsemblBacteria" id="BAD41412">
        <property type="protein sequence ID" value="BAD41412"/>
        <property type="gene ID" value="STH2427"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2427"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_4_9"/>
    <dbReference type="OMA" id="LNEHEMT"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="16107" checksum="B2BD0EF722CE9553" modified="2004-10-11" version="1">MESARIEAYLPKLEASFDNMVRVMTAATTAYQEVAPGPRMSMSQMIVLKALKARGPLQVTEVANELTVTLSAATGLVDRLVKAGFAERERDQRDRRVVWVRITPEGEQALEAAIQRRRAAFREMVKNLTEAELEQLCDILGRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LP2</accession>
    <name>Q67LP2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196542.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2419"/>
    <dbReference type="EnsemblBacteria" id="BAD41404">
        <property type="protein sequence ID" value="BAD41404"/>
        <property type="gene ID" value="STH2419"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2419"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_19_8_9"/>
    <dbReference type="OMA" id="FGMGVKD"/>
    <dbReference type="OrthoDB" id="1809950at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="18854" checksum="FD4CB3DD22EEECE5" modified="2004-10-11" version="1">MEQIGIRMARPSDAEAFAEIMSRPQVYYGTLQPPHTTPESWRKKLEGIDPRYDYVLVAEVDGKVVGNVWLQRYRNSRTLHTASLGIMVHDAWQGRGIGRALMAAAIDVADRWLNVLRVELEVFPDNERAIKLYESFGFVAEGRKRMAAFRDGQYVDTVVMGRIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS6</accession>
    <name>Q67LS6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2385"/>
    <dbReference type="EnsemblBacteria" id="BAD41370">
        <property type="protein sequence ID" value="BAD41370"/>
        <property type="gene ID" value="STH2385"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2385"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031275_8_2_9"/>
    <dbReference type="OMA" id="QEDPTDA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="39557" checksum="9F721D1CB2B51553" modified="2004-10-11" version="1">MTHRGRRILAALVLSGLVLALAWGLWTIRPVLAPFLLAVVLAYLLAPLVNRLVGLGLPRPWAILAVYAALAAAGVLVVVKLVPSALTEVRRLTEAIPLYSARAREMADGLQRWARDAGLPPELRESLDRSITNIEVRSVAALHRLVDIQTLEAAAGFLFSLALAPFLAFYLLKDLDYFRERFVRSLPRRWRQDIIQLLRELDRVISGFVRGQILLGLSVGALAAAASSLLGLRFAVLLGIWAGLMEFIPYVGPVLGAVPAVLSGLAQSPWRGLQIALVFLIIQQLENAVLSPKIMGESVGLHPIGVLLVVLAGGYLAGPWGLILAVPAAALVRVLWSFVVARLTEVHPLPVLPAPAARPEEEKGPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY0</accession>
    <name>Q67LY0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Anaerobic dimethyl sulfoxide reductase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dmsB1</name>
        <name evidence="11" type="ordered locus">STH2331</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Electron transfer subunit of the terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196454.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2331"/>
    <dbReference type="EnsemblBacteria" id="BAD41316">
        <property type="protein sequence ID" value="BAD41316"/>
        <property type="gene ID" value="STH2331"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2331"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_2_0_9"/>
    <dbReference type="OMA" id="YHYSGAC"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014297">
        <property type="entry name" value="DMSO_DmsB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02951">
        <property type="entry name" value="DMSO_dmsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="10">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD41316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="21698" checksum="C1D9B4EB4C98C3DF" modified="2004-10-11" version="1">MQYGFFIDAKYCAGCKTCQIACKDRASLEVGQLFRKVVNYEDGGWVQRGEGWVHNVTSYWFSISCNHCADPACVYVCPTGAMYKRSDNGLVLVNQDDCVGCQSCVWACPYDAPQYNPEVGRVHKCDGCIDRVEAGQRPVCVDACPYQLIDFGDIEELRAKHGGTAWVQGLADPSRTRPSLLVNPPTGAISSPIGGSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA9</accession>
    <name>Q67MA9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2199"/>
    <dbReference type="EnsemblBacteria" id="BAD41184">
        <property type="protein sequence ID" value="BAD41184"/>
        <property type="gene ID" value="STH2199"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2199"/>
    <dbReference type="eggNOG" id="ENOG5032RN5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1494616_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024047">
        <property type="entry name" value="MM3350-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012912">
        <property type="entry name" value="Plasmid_pRiA4b_Orf3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07929">
        <property type="entry name" value="PRiA4_ORF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159941">
        <property type="entry name" value="SSF159941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="17121" checksum="41E6164493BDC6A2" modified="2004-10-11" version="1">MAVRGWFTVRVDLVSGAGLHFAPQPGRIFLVGPSHTFLDLATAIDLGFARWDLSHLHEFRFPDGRCFGIPDDEDPRVVDYDTVRVASQVKRGDRFTYIFDFGANWEHHCQVLASNLDPLEVLGHVPQQPTPIWGWGWIPDQYGRRSYGAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MB8</accession>
    <name>Q67MB8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative long-chain fatty-acid-CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2190"/>
    <dbReference type="EnsemblBacteria" id="BAD41175">
        <property type="protein sequence ID" value="BAD41175"/>
        <property type="gene ID" value="STH2190"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2190"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_9"/>
    <dbReference type="OMA" id="AWPNTDF"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="20"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="435"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="523" mass="57629" checksum="05E06B32874E6E10" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC1</accession>
    <name>Q67MC1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2187"/>
    <dbReference type="EnsemblBacteria" id="BAD41172">
        <property type="protein sequence ID" value="BAD41172"/>
        <property type="gene ID" value="STH2187"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2187"/>
    <dbReference type="eggNOG" id="COG2607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039512_1_1_9"/>
    <dbReference type="OMA" id="WATRHNG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008533">
        <property type="entry name" value="DUF815"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05673">
        <property type="entry name" value="DUF815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="226"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="426" mass="47199" checksum="B7EFD4DC28AC7910" modified="2004-10-11" version="1">MWPQPDSLVLYQDLFAGGPGAAMAAFLRALPQGGEAARGAYLDLVRALYRAGDLPGPTGDAWQDYVIHGVLCADNAFTRAAAAGTVSGPLRDAAAWDLMHLQRCFRLSRLRTRALLGGGVADEQLPTWAGRPEADGAGPFASPAFRSMAHRLARAEAWAELVDDLAAFHRAQGFGPVARTWFLAWDGATLLPVEEPDLVDLDDLAGLEEQKAAIRENTEAFLRGGVGQNLLLYGPRGTGKSSLVRALALRYGEAGLRLVEVSRDRLGSLGDLYRTVRRYPQRFVLFLDDLTFEADDAGYRAFKSLLEGALARRPPNLVLYVTTNRRHMVPERWSDRNGPEEAEVHGQDALEERLSLADRFGRTILFLRPDQEEYLRIVEHLAARRGLPVGREELREAARRWALWQNGLSGRTARHFVDELEARLRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD4</accession>
    <name>ADDB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent helicase/deoxyribonuclease subunit B</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent helicase/nuclease AddB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">addB</name>
        <name type="ordered locus">STH2174</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -&gt; 3' nuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of AddA and AddB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. AddB/RexB type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MD4"/>
    <dbReference type="SMR" id="Q67MD4"/>
    <dbReference type="STRING" id="292459.STH2174"/>
    <dbReference type="PRIDE" id="Q67MD4"/>
    <dbReference type="EnsemblBacteria" id="BAD41159">
        <property type="protein sequence ID" value="BAD41159"/>
        <property type="gene ID" value="STH2174"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2174"/>
    <dbReference type="eggNOG" id="COG3857">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007838_0_0_9"/>
    <dbReference type="OMA" id="DRLENYV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01452">
        <property type="entry name" value="AddB_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014140">
        <property type="entry name" value="DNA_helicase_suAddB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02773">
        <property type="entry name" value="addB_Gpos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000379223" description="ATP-dependent helicase/deoxyribonuclease subunit B">
        <location>
            <begin position="1"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="1">
        <location>
            <begin position="282"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="803"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1121"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1124"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01452"/>
        </source>
    </evidence>
    <sequence length="1165" mass="130035" checksum="156756FAFECDF015" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME2</accession>
    <name>Q67ME2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 2-hydroxyacid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2166"/>
    <dbReference type="EnsemblBacteria" id="BAD41151">
        <property type="protein sequence ID" value="BAD41151"/>
        <property type="gene ID" value="STH2166"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2166"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_0_9"/>
    <dbReference type="OMA" id="WHHGTDK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh_C" evidence="1">
        <location>
            <begin position="104"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="33811" checksum="98FA69D367C5CE1D" modified="2004-10-11" version="1">MILVYEPKRAGTYADLLERAIPNPADYRLIPCTSREEAERAIGEAEILFCFRFPADLLQGAHRLRWIQAMAAGVDDLVAAPLPPGVLLTRIEGLFGTPMSEYAFAHMLAYAQQLRRVYAAQAERRWDPFRAETLAGKRLGVAGIGSIGLEVAVRGKAFGMEVWGLARTPGPRPHVDRVFTPDQVREFTAGVDFLVSTLPDTPETRGLIDPEGMKEGALLINVGRGATLDEGALLRAVRSGRIRAVLDVFAVEPLPGDHLFWTEPGITVTPHMSGLNRPDAIAAYCAENLRRFERGEPLRGVVDLARGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME9</accession>
    <name>Q67ME9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2159"/>
    <dbReference type="EnsemblBacteria" id="BAD41144">
        <property type="protein sequence ID" value="BAD41144"/>
        <property type="gene ID" value="STH2159"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2159"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="EVMFGPL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="7"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="274" mass="29634" checksum="0392C10D0CC40E42" modified="2004-10-11" version="1">MSGLAEIIVRDLRFHYTPGVEVLRGIDLTLTPGSVAIIGQNGAGKSTFVRLLNGLLKPTGGEVLVDGVSTRSTTVATMARKVGLVFQNPSDQLFKSRVLDEVMFGPLNLGLSPEEARRRAEQALERVGLTGVEQHNPYDLGLAERKLVTIAGVLAMDTPVVILDEPTIAQDVNGVRQIGEIVTWLQAQGRTVITITHDMDFVARYFQRVLVFQDGQVLADGTAQEIFAQPDLLRKAGLEPPPITLLGQGLGLPDPPLTVEAFVARVRALQARKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF0</accession>
    <name>Q67MF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalt ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2158</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196283.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2158"/>
    <dbReference type="EnsemblBacteria" id="BAD41143">
        <property type="protein sequence ID" value="BAD41143"/>
        <property type="gene ID" value="STH2158"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2158"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="ELIHVDC"/>
    <dbReference type="OrthoDB" id="1713578at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="30790" checksum="D3C49E7F424CFD94" modified="2004-10-11" version="1">MSVIVVENLRYRYPRQTHLALDGVSFRVEPGEFVGIVGPNKAGKSTLCQALVGLVPHFYKGAIGGRVEVAGLDVHASTVAEVSRRAGLVFQSPFTQMSGAKFTVYEEVAFGLEHTGIPRDEMVRRIEWALRLLGLWELRDRNPFALSGGQMQRLAIASVLVMQPEVLILDEPTSQLDPAGTSEVFAAVADLCRQGMTVVMAEHKVDRIAAQADKVLVLDRGQVVAWGPPGEVFSLDGLTARGVAVPAVTAAARELGWQEPDGTYPVTVEQAVRIGGERLGRDHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG0</accession>
    <name>Q67MG0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2148"/>
    <dbReference type="EnsemblBacteria" id="BAD41133">
        <property type="protein sequence ID" value="BAD41133"/>
        <property type="gene ID" value="STH2148"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2148"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1209291_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25298" checksum="1FDFE5A1F297B65D" modified="2004-10-11" version="1">MPTGSLGGRLLENGDRGFRFEWPLGGWVSVVEIVLDPVTGEGGSSDQLTREGRTVGSGTGVGHAGAGCTRVTVHHRGIPEDALPARWPEQTWQCVWVLWLRLLQGWVERAAALPRFAFRPPFGAVVERSVEIDAPPERVWAALVDPVLRDRWLGVALGPELRREEGRLLVCVFAADQPATTVTWRIDPLPDGRTRVTVREEGLGWESIDNHLGWHDWLVALALEMGRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH2</accession>
    <name>Q67MH2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2136"/>
    <dbReference type="EnsemblBacteria" id="BAD41121">
        <property type="protein sequence ID" value="BAD41121"/>
        <property type="gene ID" value="STH2136"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2136"/>
    <dbReference type="HOGENOM" id="CLU_2014104_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13811" checksum="47A5D557B7BE9FC3" modified="2004-10-11" version="1">MLGPVHPLPARRPGTAVAPHRGRRANERRRLSVYRIQKGTRKSGAGRHVAVETLRLLLLLQFAALLYLIYRVHRLEQLVNGRIRTTPRLTQRSGRAGKVVPLLRDDIPPGPFSSGNPDRDDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML9</accession>
    <name>Q67ML9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196214.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2089"/>
    <dbReference type="EnsemblBacteria" id="BAD41074">
        <property type="protein sequence ID" value="BAD41074"/>
        <property type="gene ID" value="STH2089"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2089"/>
    <dbReference type="eggNOG" id="COG2984">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058196_3_2_9"/>
    <dbReference type="OMA" id="TGLNNYH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06325">
        <property type="entry name" value="PBP1_ABC_unchar_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007487">
        <property type="entry name" value="ABC_transpt-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35271">
        <property type="entry name" value="PTHR35271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04392">
        <property type="entry name" value="ABC_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="360" mass="37230" checksum="A2339F0A1D21A80D" modified="2004-10-11" version="1">MDQRIDRNGRHRRRPGLCGPGRHVALLSLVLAAALFAGCARRDPAPAAIAVLLADDVRLEKVEGLREGLAELGYTPDRVDITVYSARGDRSRLPALAAQAIASGPDVLVAGGGIEADTLHRSDNPGLPVVMAGVASPVRWGWIDSYARPGGLMTGVDNQHAELSGKRLELLTKLLPDVRRVLVLYDPQVVPGLHALEVVEEAAARLGVSLGTVEAGDLDGALAGLGAIEPGEYDAALLLPAFVLESGAGAIAAALERLGLPAMGPLDLGPEAGLMAAYGNSNRAQGKQAARFVAKVLSGADPATLPIESIDNPELVVDLEVVRRLGITLKPEGMAFAHVRGDEAGPGPGDVQVPAGEVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MM7</accession>
    <name>Q67MM7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin oxidoreductase molybdopterin-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2081"/>
    <dbReference type="EnsemblBacteria" id="BAD41066">
        <property type="protein sequence ID" value="BAD41066"/>
        <property type="gene ID" value="STH2081"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2081"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008235_0_0_9"/>
    <dbReference type="OMA" id="ERYSGCP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02780">
        <property type="entry name" value="MopB_CT_Tetrathionate_Arsenate-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02758">
        <property type="entry name" value="MopB_Tetrathionate-Ra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037946">
        <property type="entry name" value="MopB_CT_Tetrathionate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041929">
        <property type="entry name" value="Tetrathionate-R_A_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="5">
        <location>
            <begin position="34"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1001" mass="110708" checksum="A6D361A3277EC150" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR0</accession>
    <name>Q67MR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2048"/>
    <dbReference type="EnsemblBacteria" id="BAD41033">
        <property type="protein sequence ID" value="BAD41033"/>
        <property type="gene ID" value="STH2048"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2048"/>
    <dbReference type="eggNOG" id="ENOG50321AX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128120_1_0_9"/>
    <dbReference type="OMA" id="RACHIYL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="16708" checksum="4F8A9936E1683FDB" modified="2004-10-11" version="1">MRVLLWFLGRGACACARLDSRVAAEVAGWAEGQRLCLRIGSAGPAMALAKEGGRLRFLGLRTVPEADLVIHFKHLEGALPVFLGQQSIAGAYAQRRMTLRGDLTFAMSAVRVLLITEAYLFPGIITRRIMQRVPRREVSMGRVYLRTLLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV3</accession>
    <name>Q67MV3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Germane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2005"/>
    <dbReference type="EnsemblBacteria" id="BAD40990">
        <property type="protein sequence ID" value="BAD40990"/>
        <property type="gene ID" value="STH2005"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2005"/>
    <dbReference type="eggNOG" id="COG5401">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_563746_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019606">
        <property type="entry name" value="GerMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018911">
        <property type="entry name" value="Gmad2_Ig-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10646">
        <property type="entry name" value="Germane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10648">
        <property type="entry name" value="Gmad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00909">
        <property type="entry name" value="Germane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269453" description="Germane domain-containing protein" evidence="1">
        <location>
            <begin position="34"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Germane" evidence="2">
        <location>
            <begin position="95"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="505" mass="54308" checksum="DE06D07BFEC4F014" modified="2004-10-11" version="1">MEMNPRKEQMSMSKAGPKAFARALPLMLAVLLAASGCAGVRPQPSPEGEAPVSPSVQVDPPVQTTTVTVFYPDWQVQHLIPEQRQVPEGSAAELATRVVEELLAGPADPHLKPAFPEGTRLLEPVKLEGGQATVNFSGELDQVQGSAAVMAALHSLRLSLGDIPGIDEVRIQVSGGSGGELDGMVVETKSPYLYLYPVFPNPERTRYLQSRADQGLDTWRLDPAAVARFEGRMFGFTAAELQQATVRQDGGRAEAYVTRDGTTYQFTLVRNPAAGEKGVWTIDSLNPMQWHEYDAVPAAVRELADRYAATTIGAAVAHSDRLYLIASVEEGEVRITSAEADGDALVVRVAEDGGQRLSIASVPLPGAAPDVVFRWEAEPTGPVAQHTPAEELPRLYTAEGVGHPEVKLTGDAVIASAVSEPGRIAVKGYARHLFEATVVARMLDGQGQEVAVAHATAAAAMWNWGEFTIVLEHHAPPGEYRVEVGDYSMRDGVWQPRAVTYVTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N72</accession>
    <name>Q67N72_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1886"/>
    <dbReference type="EnsemblBacteria" id="BAD40871">
        <property type="protein sequence ID" value="BAD40871"/>
        <property type="gene ID" value="STH1886"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1886"/>
    <dbReference type="HOGENOM" id="CLU_1969408_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="127" mass="13369" checksum="D7F03E457D7FB9AB" modified="2004-10-11" version="1">MRGSAGAEGGHAQNLHPGSGRRFRFCAGAPGQRGGHAQNLHPGSGRRFRFCAGAPGQKGGHAQNLHPGSGRRFRFCAGAPGQKGRHAQNLHPGSGGRFRFCAGAPGQKGRHAQNLHPDSGRRFRFCA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NA9</accession>
    <name>NUSB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nusB</name>
        <name type="ordered locus">STH1849</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NusB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195977.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NA9"/>
    <dbReference type="SMR" id="Q67NA9"/>
    <dbReference type="STRING" id="292459.STH1849"/>
    <dbReference type="EnsemblBacteria" id="BAD40834">
        <property type="protein sequence ID" value="BAD40834"/>
        <property type="gene ID" value="STH1849"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1849"/>
    <dbReference type="eggNOG" id="COG0781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087843_3_3_9"/>
    <dbReference type="OMA" id="DRMPVVD"/>
    <dbReference type="OrthoDB" id="1574817at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0889">Transcription antitermination</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000265610" description="Transcription antitermination protein NusB">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <sequence length="153" mass="16960" checksum="29EF79ED34609E37" modified="2004-10-11" version="1">MSRRKARELALQALFQMDIAGTDPDTAVAQALTRETEPDWAPDRLEEESAEFARRLVRGAWQHREESDRLIAQYARGWRVERMAAVDRAILRMAVYEIVHSEDVPDSVAVAEAVELAKTFSTADSSRFVNGILGSVIRGMKGAAGADEAVSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND7</accession>
    <name>Q67ND7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Tyrosine recombinase XerD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">xerD</name>
        <name evidence="16" type="ordered locus">STH1821</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'phage' integrase family. XerC subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the 'phage' integrase family. XerD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195949.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1821"/>
    <dbReference type="EnsemblBacteria" id="BAD40806">
        <property type="protein sequence ID" value="BAD40806"/>
        <property type="gene ID" value="STH1821"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1821"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_6_9"/>
    <dbReference type="OMA" id="HSFASHM"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01808">
        <property type="entry name" value="Recomb_XerC_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01807">
        <property type="entry name" value="Recomb_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011931">
        <property type="entry name" value="Recomb_XerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011932">
        <property type="entry name" value="Recomb_XerD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023009">
        <property type="entry name" value="Tyrosine_recombinase_XerC/XerD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02224">
        <property type="entry name" value="recomb_XerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02225">
        <property type="entry name" value="recomb_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 12" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10 12" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="15">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="14">
        <location>
            <begin position="105"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="12">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD40806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="294" mass="33724" checksum="17D4A6AA08CA997E" modified="2004-10-11" version="1">MNNLIHEFINYLSVERGLATNTLESYGRDLRQYYQYLGEDQVDLDAVSRSTIINYLMYLQKQGKATATIARRLAALKAFYQFLVREKRIKTDPTANLESPKLEKRLPRVLTVSEVERLLAQPDPSLPAGLRDRAMLELLYATGIRVSELVSLNIEDVNLETGYIRCTGKGSKERIVPLGSLAIKWVKEYLQQGRPKLVKDREEAALFVNHHGHRLTRQGFWKIVKKYAEDARIDKEITPHTLRHSFATHLLENGADLRSVQEMLGHADISTTQIYTHVTKGRLKEVYARTHPRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND8</accession>
    <name>DEOB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphopentomutase</fullName>
            <ecNumber evidence="1">5.4.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphodeoxyribomutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">deoB</name>
        <name type="ordered locus">STH1820</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphotransfer between the C1 and C5 carbon atoms of pentose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-ribose 1-phosphate = D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:18793"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="5.4.2.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-deoxy-alpha-D-ribose 1-phosphate = 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27658"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="5.4.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 or 2 manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphopentomutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195948.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67ND8"/>
    <dbReference type="SMR" id="Q67ND8"/>
    <dbReference type="STRING" id="292459.STH1820"/>
    <dbReference type="EnsemblBacteria" id="BAD40805">
        <property type="protein sequence ID" value="BAD40805"/>
        <property type="gene ID" value="STH1820"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1820"/>
    <dbReference type="eggNOG" id="COG1015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053861_0_0_9"/>
    <dbReference type="OMA" id="YLGNCHA"/>
    <dbReference type="OrthoDB" id="1772797at2"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00173"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043094">
        <property type="term" value="P:cellular metabolic compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16009">
        <property type="entry name" value="PPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00740">
        <property type="entry name" value="Phosphopentomut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010045">
        <property type="entry name" value="DeoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024052">
        <property type="entry name" value="Phosphopentomutase_DeoB_cap_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21110">
        <property type="entry name" value="PTHR21110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001491">
        <property type="entry name" value="Ppentomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143856">
        <property type="entry name" value="SSF143856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01696">
        <property type="entry name" value="deoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000258319" description="Phosphopentomutase">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00740"/>
        </source>
    </evidence>
    <sequence length="393" mass="42605" checksum="657D26B505D1A398" modified="2004-10-11" version="1">MAAVRRVVLIVIDSVGIGAMPDAADWGDAGANTLGNMARQRGGLPLPNLGRLGLGNLCAIEGTPPVDAPAGAYGRMAIFSHGKDTMTGHWEMVGIRPEAPFRTYPDGFPEDLIAEFCRRAGLEGVLGNKVASGTEIIKELGEEHLRTGWPIVYTSADSVFQVAAHEERFGLERLYEVCRIARDLLRPPHRVGRVIARPFVGTDRTNFTRTANRHDYALEPPRMLLDEVKDAGLSVLAVGKISDIFSGHGITYSVHTKSNADGIEKIHECLDRQEPGLIFANLVDFDMLYGHRRDVEGYARAMLEFDAALPAIMAKLGPEDVLVVTADHGNDPTYIGTDHTREYVPVLLYGEPIKPGVDVGTRASLADLGATVADLLGVPQTGFGESFADQIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NG5</accession>
    <name>Q67NG5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195921.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1793"/>
    <dbReference type="EnsemblBacteria" id="BAD40778">
        <property type="protein sequence ID" value="BAD40778"/>
        <property type="gene ID" value="STH1793"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1793"/>
    <dbReference type="eggNOG" id="COG3427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046420_1_1_9"/>
    <dbReference type="OrthoDB" id="1719670at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010419">
        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588">
        <property type="entry name" value="PTHR38588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="16377" checksum="6096E27A3225E331" modified="2004-10-11" version="1">MRVAGQFRFDAPPEIMYRLFSNKDALLNATIGLQSLEEVEPDRWNATLKVGIGGFALVYHGILEVTDRVPNESFHLHIAAETHNGSAEAEADLRFLPDGDGTLVTYEADIEFLGAQKLLPSLAKGLVDYFMHGMREYLEKARARALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ3</accession>
    <name>Q67NJ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1765"/>
    <dbReference type="EnsemblBacteria" id="BAD40750">
        <property type="protein sequence ID" value="BAD40750"/>
        <property type="gene ID" value="STH1765"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1765"/>
    <dbReference type="eggNOG" id="COG0724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_8_9"/>
    <dbReference type="OMA" id="ATWMGRD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="9"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="94" mass="10420" checksum="5C9FBFA52F56B8D7" modified="2004-10-11" version="1">MGERMRMTKTLYFGNLPWAVTPEDLQNAVSQHAEVLGARIATDRETGRSRGFGFVEVPADQAQAVIDALNGAEWAGRQLTVNEAQPRPERGPRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ9</accession>
    <name>MIAB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase</fullName>
            <ecNumber evidence="1">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-i(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaB</name>
        <name type="ordered locus">STH1759</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195887.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NJ9"/>
    <dbReference type="SMR" id="Q67NJ9"/>
    <dbReference type="STRING" id="292459.STH1759"/>
    <dbReference type="EnsemblBacteria" id="BAD40744">
        <property type="protein sequence ID" value="BAD40744"/>
        <property type="gene ID" value="STH1759"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1759"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_2_0_9"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000374591" description="tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase">
        <location>
            <begin position="1"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="162"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="401"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="470" mass="53517" checksum="C1690CDF01D956DF" modified="2004-10-11" version="1">MAQMQIDPEAGRRIHGKPEPRVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLLLFNTCAVRESAVEHAFGRIGQLKPLKYTNPDLIIGVCGCVPQVEGQVERIKRMFPYLDLIFGTHNIHRLPELVERARSERETVVDVWESMGDDFPDILPAAREGDLKAWVTIMYGCDKHCTYCIVPTTRGKERSRPYEVILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDFGDLIELIDRNSPGIERIRFTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRMKRSYNRKQYLRLVGWIRELIPDAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFMYSERAGTPAAQMEDRLSVPEKKERLQRLMEVQNRIARAKNEARVGKVYDILVEGLDKGKPDVVFGWTRGNILVTFPGDESLRGRIVPVRITRAGTWTLEGELVESPVTLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NP3</accession>
    <name>PSD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="1">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Phosphatidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Phosphatidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">psd</name>
        <name type="ordered locus">STH1715</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NP3"/>
    <dbReference type="SMR" id="Q67NP3"/>
    <dbReference type="STRING" id="292459.STH1715"/>
    <dbReference type="EnsemblBacteria" id="BAD40700">
        <property type="protein sequence ID" value="BAD40700"/>
        <property type="gene ID" value="STH1715"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1715"/>
    <dbReference type="eggNOG" id="COG0688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072492_2_0_9"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000029813" description="Phosphatidylserine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000029814" description="Phosphatidylserine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="183"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="1">
        <location>
            <begin position="182"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <sequence length="215" mass="23744" checksum="C502881B59E6D8E9" modified="2004-10-11" version="1">MSDMRRPIIAREGWPFVGALVALAVLALFVHWALGAVLAGLALFVMWFFRDPERPIPREDGLVVSPADGRVMFVREVEEPRFVGGRALLVSIFLSVFDVHINRSPVAGEVTYREYVPGKFLAAWDDTVGEVNERAYLGLVTDGGHRVLVSQVAGLLARRIVTWPAVGDRLDRGQRFGLIRFGSCTQVWLPADSEVLVRPGDRVVAGQTVIGRLPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-21" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P86</accession>
    <name>IF2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">STH1522</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P86"/>
    <dbReference type="SMR" id="Q67P86"/>
    <dbReference type="STRING" id="292459.STH1522"/>
    <dbReference type="EnsemblBacteria" id="BAD40507">
        <property type="protein sequence ID" value="BAD40507"/>
        <property type="gene ID" value="STH1522"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1522"/>
    <dbReference type="eggNOG" id="COG0532">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_5_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000228252" description="Translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="546"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="55"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="555"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="580"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="601"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="655"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="691"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="102"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="404"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="555"/>
            <end position="562"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="601"/>
            <end position="605"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="655"/>
            <end position="658"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1044" mass="110586" checksum="A60569269F5717D4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P90</accession>
    <name>RIMP_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimP</name>
        <name type="ordered locus">STH1518</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195648.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P90"/>
    <dbReference type="SMR" id="Q67P90"/>
    <dbReference type="STRING" id="292459.STH1518"/>
    <dbReference type="EnsemblBacteria" id="BAD40503">
        <property type="protein sequence ID" value="BAD40503"/>
        <property type="gene ID" value="STH1518"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1518"/>
    <dbReference type="eggNOG" id="COG0779">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070525_2_2_9"/>
    <dbReference type="OMA" id="YIHVSLY"/>
    <dbReference type="OrthoDB" id="1595498at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000229285" description="Ribosome maturation factor RimP">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <sequence length="160" mass="17542" checksum="2D3CE1439C64A4AD" modified="2004-10-11" version="1">MAKSGKRLEELVEELAAPHAAALGLELVGVELVKEGAYRYLRVYIDKEGGVGFDDCEALSRVVDAQLDEILPNPPYDFFEVSSPGLDRPLKREADFARYVGHKVVVTTYAPVDGQKSFVGELQGLVDGRVTLTLTEGKGRGQTIALDRKQVASARLYVEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG1</accession>
    <name>PLSX_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsX</name>
        <name type="ordered locus">STH1447</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195577.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PG1"/>
    <dbReference type="SMR" id="Q67PG1"/>
    <dbReference type="STRING" id="292459.STH1447"/>
    <dbReference type="EnsemblBacteria" id="BAD40432">
        <property type="protein sequence ID" value="BAD40432"/>
        <property type="gene ID" value="STH1447"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1447"/>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039379_1_1_9"/>
    <dbReference type="OMA" id="HGKSNAR"/>
    <dbReference type="OrthoDB" id="631784at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000189952" description="Phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <sequence length="346" mass="36332" checksum="F2CF80C9C01FA3AF" modified="2004-10-11" version="1">MGFRIAVDAMGSDAAPAPEVSGTIQAAREWPDLRFVLLGDDSRIRAEAEKAGGLPGNVEVVHTTQVITPDEEPTKAVRSKKDAPLTVAARLVKEGQADALLSAGSTGALVVVGTLGIGRMKGIDRPALGTIMPTVKQPVFMLDVGATPDARPEWLVQFALMGDIYAREILGLSRPRVALLSNGTEAEKGNAVVKATYPLLQELPSIHFIGNIEARDVPFGGADVVVADGFPGNVLLKTYEGVAMALFQSLKEALTGSPLTAVGAALAKPGLKKMAKRFDYTEYGGALLLGLKAPVVKCHGSSNARAIYSGLRVMKLALEGRVIDRLAEAIAQLPAKNPKEEGDPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG2</accession>
    <name>Q67PG2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1446"/>
    <dbReference type="EnsemblBacteria" id="BAD40431">
        <property type="protein sequence ID" value="BAD40431"/>
        <property type="gene ID" value="STH1446"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1446"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095708_0_0_9"/>
    <dbReference type="OMA" id="GIARGHH"/>
    <dbReference type="OrthoDB" id="1813646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045717">
        <property type="term" value="P:negative regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017275">
        <property type="entry name" value="Transcription_factor_FapR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037733">
        <property type="entry name" value="Transcription_factor_FapR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="123"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="21171" checksum="F905A0E8946C5D0E" modified="2004-10-11" version="1">MKKRDRQAALREKIRENPFLSDEELAHIFGVSIQTIRLDRLALGIPELRERTKSVAERTYGIVKSMGSKEIVGELIDIVLGERGISILETTEDMVFERSRVVRGQFIYAQAESLAIALIDADVVLTGLANIKFKRPVTVGEKLVAKGEVIRRRRNQHVVLVETRVGTENVFRGKFSVFEVDPARVGGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PG4</accession>
    <name>Q67PG4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195574.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1444"/>
    <dbReference type="EnsemblBacteria" id="BAD40429">
        <property type="protein sequence ID" value="BAD40429"/>
        <property type="gene ID" value="STH1444"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1444"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140876_0_0_9"/>
    <dbReference type="OMA" id="NECEIIS"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="18047" checksum="A94C81E61AA2AB28" modified="2004-10-11" version="1">MHPYRIQPPRTLADQQWLRDLWTAEWGGETMITRGRVHRLADLQALIAWDGAAPVGAATFHVEGTDCELMSLNAIRPGGGVGSALLQAVEDAARQAGCRRVWLITSNDNLDALRFYQRRGYRLVAVHPGAIDEARRLKPTIPLVGEHGIPIHDELELEKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL6</accession>
    <name>Q67PL6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1392"/>
    <dbReference type="ESTHER" id="symth-q67pl6">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40377">
        <property type="protein sequence ID" value="BAD40377"/>
        <property type="gene ID" value="STH1392"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1392"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_44_0_9"/>
    <dbReference type="OMA" id="FAMINSA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="28"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="268" mass="29117" checksum="2FD4CAEFE688ED13" modified="2004-10-11" version="1">MWKSPSGRRRHPMSLPCGIFGPPLAPAVLFLHGGGVSGWMWGPVVSRLQHRFRCLVPDLPGHGERTRETFTFGRAVAGLADLVRRVADGPVHVVGVSLGGQTALRLIAAHPELVGRALVSGTLTRPLPGVAPYRFLLRLTYPLARREWMIRANARALGIPPAYLEPFRSDTLRATAAGLEAVVAENMAFRPPEALSTSPVPLLALVGERERRLLRACARDLARTMPHARAYLVPGAGHTWCLEHPDLFARAVAAHLSDEPLPAELVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ2</accession>
    <name>Q67PQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chemotaxis signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheW1</name>
        <name evidence="2" type="ordered locus">STH1356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1356"/>
    <dbReference type="EnsemblBacteria" id="BAD40341">
        <property type="protein sequence ID" value="BAD40341"/>
        <property type="gene ID" value="STH1356"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1356"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_3_1_9"/>
    <dbReference type="OMA" id="FIQGVCH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="15704" checksum="C24C7AF419AD7889" modified="2004-10-11" version="1">MHSMAAGQYVIFRLDEQLYGADIAVVREVSYLAPVTRLPNTPHFVEGVIDLRGEVMPVIDLRKRLGLPSRPADGDSRIMILTVGGVTAALVVDGVEQVATIADEAVSLPDDSLTVVGQDYVYGVARMNGRLVVLIDLSRMLSIAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR7</accession>
    <name>Q67PR7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="7" type="ordered locus">STH1341</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195472.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1341"/>
    <dbReference type="EnsemblBacteria" id="BAD40326">
        <property type="protein sequence ID" value="BAD40326"/>
        <property type="gene ID" value="STH1341"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1341"/>
    <dbReference type="eggNOG" id="COG0452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_1_9"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="OrthoDB" id="1346419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="5"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="193"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="198"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="312"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="414" mass="43954" checksum="EE403E2A1C08C78C" modified="2004-10-11" version="1">MLHGKTVLLGVSGGIAAYKAAEICSRLVKLGADVHVLMTEAATRLVAPLTFQALSGNPVKVDLMGEQVHGHVDHVLLTHKADLLIIAPATANTIARLAGGHAGDWITVTALGVRCPVLVAPSMETEMYANPLTQRNLRILAELGWDIMEPEEGRLASGLMGKGRLPEPAKIVERAVALLQAREGRQRRPGGDLAGRRVLVTAGTTREPFDPVRFIGNRSTGRMGYAIAEAARDRGAEVVLVSGPTHLEPPAGVRLVRVESCVQMLEACLAEFPAADVAVAAAAPADYRPATVAPSKIKKTGEELTITLVKNPDIIAELGRRKRPGQVTVAFAAETDDLIANARRKLADKNADFVVANDVTAEGAGFGTETNRVVFVTADAVDELPLLPKREVADRILDRAAALLEDGARGHGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU0</accession>
    <name>Q67PU0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195449.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1318"/>
    <dbReference type="EnsemblBacteria" id="BAD40303">
        <property type="protein sequence ID" value="BAD40303"/>
        <property type="gene ID" value="STH1318"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1318"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_9"/>
    <dbReference type="OMA" id="MRRHTDG"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="32710" checksum="CF905EBFF24D04FE" modified="2004-10-11" version="1">MLLQHLITFCRVVETGGFARAGALVGLSQPAVTRQVAALEAELGTPLLDRSGRQIHLTPAGRLVYERARRIITAVNTLKEEVADLAHPERGRIAIGAVTTVGLRLLPPILAAFKREHPGIRVHVKADRTQETIQRLLEGEIELAILPTPVTHPRLRSVPLVEDPVVLVCAPGRRSELPDPLPLAKLGEIDLISFQAPSRFRTFVDAMLEQAGVYPNVTMEFDSHEAVTLMVEAGFGCALVPASAVERELALGTVVRVEVEGMPRMARTTSLAFRHPNPGWSPATERLFQMIVDFFRQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV8</accession>
    <name>Q67PV8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transposase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1300"/>
    <dbReference type="EnsemblBacteria" id="BAD40285">
        <property type="protein sequence ID" value="BAD40285"/>
        <property type="gene ID" value="STH1300"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1300"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_7_1_9"/>
    <dbReference type="OMA" id="ACLHREI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017894">
        <property type="entry name" value="HTH_IS21_transposase_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50531">
        <property type="entry name" value="HTH_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="HTH IS21-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="134" mass="15473" checksum="AF7D9369381DB611" modified="2004-10-11" version="1">MYAAGLSISEIARRTGRDRKTIRKWLRTNAMPKPAKRKRSSMLDQHKAFILEQMQKGVTNASKMLYLLQQRGFKGKIRIVRAFMAPYRPMAKAAATVRFETPPGKQAQVDWADFGYIEVDGRRLKLYCFIMVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW3</accession>
    <name>Q67PW3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1295"/>
    <dbReference type="EnsemblBacteria" id="BAD40280">
        <property type="protein sequence ID" value="BAD40280"/>
        <property type="gene ID" value="STH1295"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1295"/>
    <dbReference type="HOGENOM" id="CLU_2385052_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="94" mass="10379" checksum="4DBE734C37AB0636" modified="2004-10-11" version="1">MGGRLSPQTVVDFIRNKWTDSTGMSGRIRPEYARAGRTFVASSVMEARVSALERTVTQHDKRIGTLDRLQTTTEATDAAMQQDIGEAKEAAQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q06</accession>
    <name>Q67Q06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tagatose-6-phosphate aldose/ketose isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">agaS</name>
        <name evidence="6" type="ordered locus">STH1252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-galactosamine 6-phosphate + H2O = D-tagatopyranose 1-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:47680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:71674"/>
            <dbReference type="ChEBI" id="CHEBI:138150"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. AgaS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195383.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1252"/>
    <dbReference type="EnsemblBacteria" id="BAD40237">
        <property type="protein sequence ID" value="BAD40237"/>
        <property type="gene ID" value="STH1252"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1252"/>
    <dbReference type="eggNOG" id="COG2222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_0_0_9"/>
    <dbReference type="OMA" id="WTVGLAH"/>
    <dbReference type="OrthoDB" id="1046291at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05010">
        <property type="entry name" value="SIS_AgaS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035464">
        <property type="entry name" value="SIS_AgaS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SIS" evidence="5">
        <location>
            <begin position="48"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="5">
        <location>
            <begin position="223"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="401" mass="43243" checksum="577E274D5BF7D22D" modified="2004-10-11" version="1">MHILAASTEELRALGAELTAREIAQQPELWAETFAAYREQQERIERFLHDVTRRHGRLRVLFAGAGSSAYVGDVLLPFVKLSGDEGAFEFQATPTTSIVSNPASYLRRDLPTLLVSFARSGNSPESTAAVALARDLVQDLYQITLTCAPEGQLARQAGGDPRNLLWLMPERSNDQGFAMTGSFTCMTLAGLLIFDRRPLDEKARLVDQICAMGQEVIAREPEIGALANLGFRRIVYLGSGPLAGIAREAQLKILELTAGKIATAFDTPLGFRHGPKSFVDEHSAVFVLVANDPYTRKYDLDMLRELRADGIARAVCAVAVKHGGDEDAGAGAPQPSFRFPAAHAGVPDGYLALPYTVFGQILALHAAVRLGNRPDTPSPTGTVNRVVRGVTIHAFPVAGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q12</accession>
    <name>Q67Q12_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tagatose-6-phosphate kinase</fullName>
            <ecNumber evidence="5">2.7.1.144</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1246</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + D-tagatofuranose 6-phosphate = ADP + D-tagatofuranose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12420"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58694"/>
            <dbReference type="ChEBI" id="CHEBI:58695"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.144"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-fructose 1-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14213"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.56"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate metabolism; D-tagatose 6-phosphate degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carbohydrate kinase PfkB family. LacC subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.144" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1246"/>
    <dbReference type="EnsemblBacteria" id="BAD40231">
        <property type="protein sequence ID" value="BAD40231"/>
        <property type="gene ID" value="STH1246"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1246"/>
    <dbReference type="eggNOG" id="COG1105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050013_0_2_9"/>
    <dbReference type="OMA" id="QLNEPGP"/>
    <dbReference type="UniPathway" id="UPA00704">
        <property type="reaction ID" value="UER00715"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008662">
        <property type="term" value="F:1-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009024">
        <property type="term" value="F:tagatose-6-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001059">
        <property type="term" value="P:D-tagatose 6-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005988">
        <property type="term" value="P:lactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01164">
        <property type="entry name" value="FruK_PfkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022463">
        <property type="entry name" value="1-PFruKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017583">
        <property type="entry name" value="Tagatose/fructose_Pkinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000535">
        <property type="entry name" value="1PFK/6PFK/LacC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03168">
        <property type="entry name" value="1-PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03828">
        <property type="entry name" value="pfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0423">Lactose metabolism</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="6">
        <location>
            <begin position="21"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="33571" checksum="8700F160D491996A" modified="2004-10-11" version="1">MGRRERPAMERYILTVTLNASVDVACTVPGFAAGKINVARAVERVAGGKGNNVARVLRRLGARVVATGFAGGSAGQFIVRDLERRGVVPAYVEVAGESRSCFAITDPESGTVTELREPGAEVSAEDVARFTAHFTRLLDGADLVAISGSAPPGADAGIYRDLVEAARSRGVRVVLDSSGAPLRAALGARPFLVKPNQAELSEWAGAPVRTEAEALDAARRMRETGPEWVLVSLGADGALLVGPDAHGWRAVPPAIRAVNTVGSGDSAVAGLTYGLAEGLPPEELLRLAVACGTANALTSGVADPDPAEVERLKREIRVERIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q35</accession>
    <name>Q67Q35_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pro-sigma-E processing factor SpoIIR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spoIIR</name>
        <name evidence="1" type="ordered locus">STH1223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195354.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1223"/>
    <dbReference type="EnsemblBacteria" id="BAD40208">
        <property type="protein sequence ID" value="BAD40208"/>
        <property type="gene ID" value="STH1223"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1223"/>
    <dbReference type="eggNOG" id="ENOG5031K93">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1293792_0_0_9"/>
    <dbReference type="OMA" id="CYTIREV"/>
    <dbReference type="OrthoDB" id="1300956at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014202">
        <property type="entry name" value="Spore_II_R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09551">
        <property type="entry name" value="Spore_II_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02837">
        <property type="entry name" value="spore_II_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="22247" checksum="7EFE71D68FD67B50" modified="2004-10-11" version="1">MNRKLITGWLLIVLGLGQAGGGVLLAAAARAEDAALADPNLVRIHVIAHSDSPEEQALKLAVRDAVLDRLRPALSGARSRAEAEARIAVLLPELEQAARAVAAAWGAAHPVRAELGTYAFPGKGYGDLYLPAGRYRALRILIGDAAGANFWCLVYPSFCYTIREVQVPPTEAVGTTLPEGCADGCAGSCTGRYPEPWAEGCPGICTAGCVSPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q63</accession>
    <name>GLPK_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol kinase</fullName>
            <ecNumber evidence="1">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glycerokinase</fullName>
            <shortName evidence="1">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glpK</name>
        <name type="ordered locus">STH1195</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer and homodimer (in equilibrium).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195326.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q63"/>
    <dbReference type="SMR" id="Q67Q63"/>
    <dbReference type="STRING" id="292459.STH1195"/>
    <dbReference type="PRIDE" id="Q67Q63"/>
    <dbReference type="EnsemblBacteria" id="BAD40180">
        <property type="protein sequence ID" value="BAD40180"/>
        <property type="gene ID" value="STH1195"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1195"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_9"/>
    <dbReference type="OMA" id="FMLMNIG"/>
    <dbReference type="OrthoDB" id="233605at2"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0319">Glycerol metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000020806" description="Glycerol kinase">
        <location>
            <begin position="1"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="410"/>
            <end position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <sequence length="498" mass="54500" checksum="BBB81EB46CD26ACA" modified="2004-10-11" version="1">MNKGYVLALDQGTTSSRAILFNRDGRLVGQVNHEFRQIYPQPGWVEHDPREIWSTQLRAVQDVLARTGVRLSEVAAIGITNQRETTVVWDAETGEPVYNAIVWQCRRTAPICAELAARGWAPRIREKTGLVIDAYFSGTKVKWILDNVPGVREKAEQGRLRFGTIDAWLIWNLTGGRVHATDYSNASRTMLFNIHQLAWDEEILHELGIPASLLPRALPSSHVYGETDPAVLGAAIPIAGVAGDQQAALFGQACFQPGDAKNTYGTGCFMLMNTGDRAVPSESGLLTTIAWGIGDRVEYALEGSIFIGGAAVQWLRDELRFFDRAADSEALALSVPDSGGVYVVPAFVGLGAPYWDMYARGIVVGLTRGTGRAHITRATLESIAYQTRDVLGAMEKDSGIRLNRLKVDGGAVANNFLMQFQSDILGVPVERPKIAETTALGAAYLAGLATGFWRSQAELADKWALDRGFTPALPAEERERLYAGWRRAVERARGWAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q90</accession>
    <name>Q67Q90_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1168</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1168"/>
    <dbReference type="EnsemblBacteria" id="BAD40153">
        <property type="protein sequence ID" value="BAD40153"/>
        <property type="gene ID" value="STH1168"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1168"/>
    <dbReference type="eggNOG" id="COG1862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116157_5_2_9"/>
    <dbReference type="OMA" id="GMEMIIM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="85" mass="9743" checksum="BBD317AF3947AE26" modified="2004-10-11" version="1">MQWGPLILLFVLMYFMMIRPQQQQQKKRQEMLNSLKVGDEIVTIGGLHGVIQAVDETNNTIRVKVASNVELTFNRSAVGTVRREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB2</accession>
    <name>Q67QB2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1146"/>
    <dbReference type="EnsemblBacteria" id="BAD40131">
        <property type="protein sequence ID" value="BAD40131"/>
        <property type="gene ID" value="STH1146"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1146"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_0_0_9"/>
    <dbReference type="OMA" id="EWADWAS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026045">
        <property type="entry name" value="Ferric-bd"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002825">
        <property type="entry name" value="CfbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269492" evidence="3">
        <location>
            <begin position="22"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="37862" checksum="1774319BA33E9C44" modified="2004-10-11" version="1">MLSLILALSVFLSGCGGSSSGSNTGGSGSPAGSTSGSTPGSGSSSGGSGPSGSLMIYTSIYPDIINLVSPELEKAFPNLKVEWFQGGTEQVTAKLAAEIEAGKIGADLLMVADPSYYVYLKDQDLLLPYKSENFDAVAVDKDTEDYAYTGVRISNVVIGYNTNLVKPEDAPKTFRELTDPKWKDQVVIVDPTLSGTALVSTYALSEKYGWEYFEQLRDNGAVIGGGNSSVETKLVTGEFAVAVILEENLLKVKDRGEPVEVVYPEDGVIIVPSPIGILKTTQNAEAAKAVVDWWLSTEGQKAIVTGWMHSVRTDVQSPKGAKPLAEFAPGAIKVDWKDLASRSGEVKDKFVEIMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL6</accession>
    <name>Q67QL6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1042"/>
    <dbReference type="EnsemblBacteria" id="BAD40027">
        <property type="protein sequence ID" value="BAD40027"/>
        <property type="gene ID" value="STH1042"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1042"/>
    <dbReference type="HOGENOM" id="CLU_1053473_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="264" mass="27402" checksum="181C2879AD6A7F82" modified="2004-10-11" version="1">MKVNHSTLQGVPVLSHLCYATGLQLLSWSLGLAATLVLLDSRSGFLRDHPPLAWRVAGVLLGPLAVLSLAAVAVGGGPRWVTLGLVLPYALMALGASWVWYRGARADLRRQREREAAVLGLLAVLFASLAQTGGDGLPVLSLAASLGSAALVGGLGILALGCTVRRRRDEVEVDPNCDGIPVRVVATGLGIMALAASDVGRALLTGSAPLPASLGLWLGCSLLVPVLVLLAGRRLSDWNRPLLWRTACLAALVGQLALQTTLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ7</accession>
    <name>Q67QQ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation efflux system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1001</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1001"/>
    <dbReference type="EnsemblBacteria" id="BAD39986">
        <property type="protein sequence ID" value="BAD39986"/>
        <property type="gene ID" value="STH1001"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1001"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_3_9"/>
    <dbReference type="OMA" id="NYGHEKA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269321" evidence="7">
        <location>
            <begin position="34"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="8">
        <location>
            <begin position="215"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="329" mass="34029" checksum="12BDFC7EB06BEB52" modified="2004-10-11" version="1">MLSSASGDRRQRLIHRAGLLTIAVNLCLTVARAAAGFLAGSTAVLADAANSGTDILATLVVMGGSRIAARPPDWNHPYGHDKAEPVAAKLVGILVTFAGLATAAGAVQALRAGGEPVGLAAAVVTAVSIAAKEALARYLARLGRRLRSQAVLADAANQRTDVLASATALAGALGGRFGLPILDPLMGLLVSALILRMGLGLYWQAVRDLMDRAPEPETVDAMRRAALSVPGVREVGDLRARVFGPGIYAECKVSVDAGLTVAEGHRIGKRVKEAVMRAVPGCRDVLVHVNPYPGLEEEGDVLPLYPVADELPAVEGAEDAWAGREGEGP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ4</accession>
    <name>CLPP2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease proteolytic subunit 2</fullName>
            <ecNumber evidence="1">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endopeptidase Clp 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpP2</name>
        <name type="ordered locus">STH914</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195046.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QZ4"/>
    <dbReference type="SMR" id="Q67QZ4"/>
    <dbReference type="STRING" id="292459.STH914"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="EnsemblBacteria" id="BAD39899">
        <property type="protein sequence ID" value="BAD39899"/>
        <property type="gene ID" value="STH914"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH914"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_2_9"/>
    <dbReference type="OMA" id="GIFDTMQ"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000179680" description="ATP-dependent Clp protease proteolytic subunit 2">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <sequence length="200" mass="22005" checksum="3410B65B3B724F45" modified="2004-10-11" version="1">MSSLIPIVIEQTSRGERSYDIYSRLLKDRIIFLGTSIDDQVANAVTAQLLFLESDDPDKEINMYINSSGGITSAGLAIYDTMQHIKPKVNTYAIGMAASMAAVLLAGGTGTRYALPHARILIHQPWVKGGIGGQVTDIEITAKELLHTKEKLAEIIAKHTGQPLEKVKEDSERDRWMSAEEAKAYGLVDVVVQPRERKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R14</accession>
    <name>Q67R14_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195026.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39879">
        <property type="protein sequence ID" value="BAD39879"/>
        <property type="gene ID" value="STH894"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH894"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_2_1_9"/>
    <dbReference type="OMA" id="QCDIRKY"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04416">
        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="429" mass="49996" checksum="3F6A3AD295E47EBD" modified="2004-10-11" version="1">MEQVVARENMLAALKRVERNGGAPGVDGVPTERLRDQIRVEWSRIREELLQGTYRPQPVRRVEIPKPGGGKRMLGIPTVMDRLIQQALLQVLTPIFDPTFSESSYGFRPGRRGHDAVRKARQYVEEGYDWVVDMDLEKFFDRVNHDVLMARVARRVTDKRVLRLIRRYLQAGVMLNGVVVATEEGTPQGGPLSPLLANILLDDLDKELERRGHHFVRYADDCNIYVRSKRAGERVYRSVRHFLQERLRLKVNEEKSAVDRPWKRQFLGFSFYKHRGVRIRLAPKSLKRVKDKLRTLTDRNRSQSMEDRIRRLNAYLRGWVGYYALSDARSAFERLEGWLKRRLRACVWRQWKRVRTRFRELRALGLPEWVVYQLANSRKGPWRMAGGPLNSALGDAYWRAQGLISLTECYEATRQSWRTAGCGPACPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R15</accession>
    <name>Q67R15_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arsenical pump membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH893</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in arsenical resistance. Thought to form the channel of an arsenite pump.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ArsB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH893"/>
    <dbReference type="EnsemblBacteria" id="BAD39878">
        <property type="protein sequence ID" value="BAD39878"/>
        <property type="gene ID" value="STH893"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH893"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043931_1_0_9"/>
    <dbReference type="OMA" id="SWGYYFK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01118">
        <property type="entry name" value="ArsB_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02040">
        <property type="entry name" value="ArsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00935">
        <property type="entry name" value="2a45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="433" mass="45329" checksum="A2B46155D76A4D64" modified="2004-10-11" version="1">METILTPAAIFAGTLFLVIRRPWNLNTGVSALLGALLALLAGSITLVDVESVIGIVWDATLALVAIMVISTLLEQAGFFRWAALHLARRAGGSGRGLFVAVILLATAVSAFFTNDATVLIVTPIVLEMVEALGFGHQALLPFAMACGFMADAMSIPLAVSNLTNMIVVRYFDLSFARFALMMLLPSLACLGVTTLVLLGYYRREIPRRVDASGLGEPAAAIRDPFLFRVGVAVVCGMILAFLFNRTAFHVPTSAIMTIGAVLLLLAALRNRVVEPWPAIRRAPWHVVAFALGMYLVVFALGKAGLTGMVAGLLQGLTARGLGVTVLGTGLLVALLSAGVNNLPGVLIGTLAVAATGAAGAIGEGMVMATVIGADIGPKLTPIGSLATLMWLHILEGRGLSVSWGRYLRTALVITPPVLLATLGALWAALRLMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE9</accession>
    <name>Q67RE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194892.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH759"/>
    <dbReference type="EnsemblBacteria" id="BAD39744">
        <property type="protein sequence ID" value="BAD39744"/>
        <property type="gene ID" value="STH759"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH759"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="237" mass="25608" checksum="BF9C948EACF6CF35" modified="2004-10-11" version="1">MLTLESVSHAFGGHPVLEDIDLRLEPGQVLAIVGRSGCGKTTLLQIASGLLSPTRGRVENRFRRLAYVFQEPRLLPWRTAWQNIAFGLKAMGAPPAEQRRVASALIERLGLAGAADKYPHELSGGMRQRVALGRALAIGPDLLLLDEPFGALDPGLRADMQRLLLDLLAERKVAALFVTHDLAEAVRVGDALIALSPAPGRIIARVPLGRTPRTDADAYVHRTVAAIWPQVSAAYPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF4</accession>
    <name>Q67RF4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oligoendopeptidase F</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH754</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Has oligopeptidase activity and degrades a variety of small bioactive peptides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194887.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH754"/>
    <dbReference type="EnsemblBacteria" id="BAD39739">
        <property type="protein sequence ID" value="BAD39739"/>
        <property type="gene ID" value="STH754"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH754"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_1_1_9"/>
    <dbReference type="OMA" id="YQREVYR"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09609">
        <property type="entry name" value="M3B_PepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034009">
        <property type="entry name" value="M3B_PepF_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004438">
        <property type="entry name" value="Peptidase_M3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00181">
        <property type="entry name" value="pepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="8">
        <location>
            <begin position="125"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="7">
        <location>
            <begin position="216"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="609" mass="68268" checksum="39F309A5F09452BF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG0</accession>
    <name>Q67RG0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH748</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194881.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH748"/>
    <dbReference type="EnsemblBacteria" id="BAD39733">
        <property type="protein sequence ID" value="BAD39733"/>
        <property type="gene ID" value="STH748"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH748"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_9"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="OrthoDB" id="1325025at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="68"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="288" mass="30526" checksum="53C13A540558B4E0" modified="2004-10-11" version="1">MDRVRTADRLARAVLTGCAVLVALLIAAILLFVGSEGLRLFADVSPLEFFLSPVWNPPLGRFGALPFMAGSLAVTGIALLISAPLGLAGAIFMSKVAPGWMRHVLRQASNLFVGIPSVVYGYVGMTLLVPWVRNHLGGQGFGVLVAGIVLAVMVLPTIIGMSEDALQAVDAAIEEGSLGLGATRWQTIWNVTLPAARPRILAAIILAFARAFGETTAVQMVIGNSPVLVRSLTKGTATLTSQIIMEMGQTPPGSPWNRALFMMAFGLLVVSLLMILLVRWVSNRGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RG8</accession>
    <name>Q67RG8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194873.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH740"/>
    <dbReference type="EnsemblBacteria" id="BAD39725">
        <property type="protein sequence ID" value="BAD39725"/>
        <property type="gene ID" value="STH740"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH740"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1562096_0_0_9"/>
    <dbReference type="OrthoDB" id="1561653at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="113"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="171" mass="17760" checksum="BF3204963FA848D0" modified="2004-10-11" version="1">MQTMTSKTRTSLVIGGVAALVLVISLAIYAVTQRSQSPAESTNPLEQIAEPENGTPLSTSPTSNPSATANPSEAGTGTSQQAAGTAPADGSDAAAGTEDGTTPAMTRLEAVPAIHIVQPNETLYEISQKYYNTHIYAGDIEALNGLENPDQIYAGMELRLPQPSELPGYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RS8</accession>
    <name>Q67RS8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mut-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194763.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH630"/>
    <dbReference type="EnsemblBacteria" id="BAD39615">
        <property type="protein sequence ID" value="BAD39615"/>
        <property type="gene ID" value="STH630"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH630"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_7_0_9"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="16770" checksum="F7F0074B57CA3A42" modified="2004-10-11" version="1">MQHLRRHLSAGGLVLHEGAILLVRNRRGHWGLPKGHWEPGELLAETAAREVREETGLEVEIGDLAFITEFRNAEAKEHLVQFFFGARLIGGSLSPAPGEISGVKWVPTSEVEQYIRWRPWLEPLRHWLNGGTVKYHAFPDPSRNRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX4</accession>
    <name>Q67RX4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-oxoacid_dh domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194718.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH584"/>
    <dbReference type="EnsemblBacteria" id="BAD39569">
        <property type="protein sequence ID" value="BAD39569"/>
        <property type="gene ID" value="STH584"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH584"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_2_1_9"/>
    <dbReference type="OMA" id="CIAKAIR"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-oxoacid_dh" evidence="2">
        <location>
            <begin position="38"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="262" mass="27913" checksum="0BD185B92D0AC15C" modified="2004-10-11" version="1">MQLFPSPPPGAPAGLLLPGGTPDNPLAMGGPPFPPVVTLPLSHIRRVSMLNLEQAQRTTAPVTVVAEVDATGLISVRESLKPLAERHLGIRLTYLPFFAAATVRALKAWPIMNAMLTPQGFVIPRYINLGIATAVPGGVLLPVVPGAERMGFWDLARAIHLQTQKARAGLLSPHELSGHTFVITNTGRYGATLFGTPIIQPPNVGILAFEAIQKRPVVVGDDQLAIRPMMYLALTADHRAVDGAEMIGFLATVKEALEQVAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ0</accession>
    <name>RECO_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein RecO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Recombination protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recO</name>
        <name type="ordered locus">STH568</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA repair and RecF pathway recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194702.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RZ0"/>
    <dbReference type="SMR" id="Q67RZ0"/>
    <dbReference type="STRING" id="292459.STH568"/>
    <dbReference type="EnsemblBacteria" id="BAD39553">
        <property type="protein sequence ID" value="BAD39553"/>
        <property type="gene ID" value="STH568"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH568"/>
    <dbReference type="eggNOG" id="COG1381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066632_3_0_9"/>
    <dbReference type="OMA" id="LCTQSET"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00201">
        <property type="entry name" value="RecO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022572">
        <property type="entry name" value="DNA_rep/recomb_RecO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003717">
        <property type="entry name" value="RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042242">
        <property type="entry name" value="RecO_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33991">
        <property type="entry name" value="PTHR33991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02565">
        <property type="entry name" value="RecO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11967">
        <property type="entry name" value="RecO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="SSF57863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00613">
        <property type="entry name" value="reco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205017" description="DNA repair protein RecO">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00201"/>
        </source>
    </evidence>
    <sequence length="253" mass="28265" checksum="FBD85A1F3D3CC392" modified="2004-10-11" version="1">MALYNVDGIVIRVRNFQDADKIVVLLTREEGKVEAVARGARRPRNRFAAATQLFSHVRAQLFSGRSLDTLSQVEIVESFRQLREDLVRMAYATYACELVDALLPERQRQEAPYLLLLTSLHLWGEPDRDPEPLLRAFELKLLSMLGFRPSLAACVGCGSEAVQQNGGVRFAPVLGGVLCPQCTDEGEGALRLSLGALETMKRLLDGDIRRAHMVRLSGELAAEIDRALSAYILARTERRLKSKEFLDTLRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S02</accession>
    <name>Q67S02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional antiterminator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH556"/>
    <dbReference type="EnsemblBacteria" id="BAD39541">
        <property type="protein sequence ID" value="BAD39541"/>
        <property type="gene ID" value="STH556"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH556"/>
    <dbReference type="eggNOG" id="COG3711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146117_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRD" evidence="1">
        <location>
            <begin position="18"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="125" mass="13967" checksum="295EB5D3BAF29EC4" modified="2004-10-11" version="1">MSPLSLEMRLRLLQESGQISPEVAELTRWILKRVEERYDMTVTEENGAMFTTHLAVALERLRSGQAIDEMVSEGLAEVKAYPEEWAFVNEVVAGEAGRRLGARVPEAEVGYLTAHLITLVRPDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S48</accession>
    <name>Q67S48_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH510"/>
    <dbReference type="EnsemblBacteria" id="BAD39495">
        <property type="protein sequence ID" value="BAD39495"/>
        <property type="gene ID" value="STH510"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH510"/>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049311_3_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="201" mass="22028" checksum="5FCE83E6984219E6" modified="2004-10-11" version="1">MLAIGPHPGDLELFAGGTLRLLSQNGSAVTLAVLSRGEQATNRANIGEIRAREAEQAATILRAELVQLDLPDGGIRPGPELERALDELWARTRPELVLAFDPKGPTPLAQNPDHLALGAAVLARARSSVWRGERIYFYAARQPNVLVDITEVLQEKLNAVKAHRSQLLGPDWAVDSFGRFVSRLYSGRVPALYTEAFYRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S52</accession>
    <name>Q67S52_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194640.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH506"/>
    <dbReference type="EnsemblBacteria" id="BAD39491">
        <property type="protein sequence ID" value="BAD39491"/>
        <property type="gene ID" value="STH506"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH506"/>
    <dbReference type="HOGENOM" id="CLU_1045557_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="29027" checksum="54524B96DEFAD871" modified="2004-10-11" version="1">MPRLNRRDPLLDPLLPPSAWEIARRRRRVRRWVALALAAALVGLGAWGWLTFTDRTRSLPPVQVAEAAGEALLRAARYDFRAAVTGSSPDGFFPNAQLAGEFQAEPLLLHLRGEVGSGAAMMPIEYWLSGDALFVRQARGGWVRSPTADVPDVVAFLPEQLAAPLLAGVNRAELVGRERVDGAQTAVLALDLDPAVMQVAPPGQDERVEYRLWVDVRRLRPVRMAIRVERPADAGSSFDYQLDLTYPAPGSLSLPDEVLQAAGPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S73</accession>
    <name>Q67S73_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CYTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH485"/>
    <dbReference type="EnsemblBacteria" id="BAD39470">
        <property type="protein sequence ID" value="BAD39470"/>
        <property type="gene ID" value="STH485"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH485"/>
    <dbReference type="eggNOG" id="COG3025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1260928_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="3"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="219" mass="23446" checksum="B1EDE1AA73BB404B" modified="2004-10-11" version="1">MTTLEVEIKLAVPPTVPGGPGALFERLAAQETLAGFPLGAAQRVALRDTYFDTDDGTLAGAGAGLRVRREDGRTLITLKVTQAREDALTRREEYEAPLDLASLREVVERIRPLIGPGTVPFAAFAAGRPAGPLEPVLDVRTERIVRTVGNVATLSLDRVLYPGLAPEPYYDIEVEAAPGLADPAVLRALEAALTAAADGHLRPEGRSKLERGLALLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG8</accession>
    <name>Q67SG8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative secretion ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194524.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH390"/>
    <dbReference type="EnsemblBacteria" id="BAD39375">
        <property type="protein sequence ID" value="BAD39375"/>
        <property type="gene ID" value="STH390"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH390"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_4_0_9"/>
    <dbReference type="OMA" id="NGPDAIF"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="93"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="423" mass="47293" checksum="8D9F57C09CF21D62" modified="2004-10-11" version="1">MSSRSLLQRRVGAALTRDPAAQVQYERLRDYVRERLLQEISGDLLLRPTDASLRLLARERITAMLDRDGSVPAAVRDTVVTRLVQDLVGFGVLQPLMDDPEVTEILVNRYDDIWVERQGRLERVEGLAFESDEAVRHLAERIAGPIRRRIDERHPILDARLADGSRVCATLSPPALDGCALAIRKFSPRMLGWQTLVETGTLSAEAADFLRRAVRARCNMLVTGGTSSGKTTVLNALSAFIPAEERVITIEDAAELRLQQPHVLRYETRSGNAEGEGAITIRDLVRTALRLRPDRIIVGEVRGAEALDMVQACNTGHDGSFSTLHANSARDGLARLETMVLMADSGLPIRAIRQQVASAFELIIHCARLRSGQRRVVEIAEVDLEDEGYHLRPLFRWNPAQDRLEPTGARPERVVRRYLWEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ1</accession>
    <name>Q67SJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Folylpolyglutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH367"/>
    <dbReference type="EnsemblBacteria" id="BAD39352">
        <property type="protein sequence ID" value="BAD39352"/>
        <property type="gene ID" value="STH367"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH367"/>
    <dbReference type="eggNOG" id="COG0285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_2_9"/>
    <dbReference type="OMA" id="KCDYAVI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="7">
        <location>
            <begin position="45"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="6">
        <location>
            <begin position="291"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="437" mass="46009" checksum="5B04D679659699C2" modified="2004-10-11" version="1">MTYEAALAYLAGLQRFGMKPGLERTVALLEQLGRPDRRVGRVVHITGTSGKGSVAAMVEAGLRAAGRRVGLYTSPPLMRFKERMQVNRAEIPEADVARLTARVAEAAEAVIAAGLEPPTEFEVITAMALAWFAEQSVDDLVLEAGMGGRFDATTAVEKAAVTAITNVGLDHTQWLGQTHEAIAWDKAGVIRPGVPCVTGTDHPGALAVIERVAREQGAPLLHVTPADFRVLGADLSGQVADLRGERGWYRRVRLSLLGRHQAANAAVALRILEVLGVDEGAIREGFAHVAWPGRMELLRAAGGQLVLLDAAHNPAKCAALAGAVAELFPGRRVHLVVGMLADKDAAQGLAPLLPLAERVWATTPESPRAMPAPELAQLCRELGHPALVAADLPEALEAALAAAAPGDLVVITGSFYTVGPARRILVPGQVIQALPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SK7</accession>
    <name>Q67SK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BglG family transcription antiterminator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH351"/>
    <dbReference type="EnsemblBacteria" id="BAD39336">
        <property type="protein sequence ID" value="BAD39336"/>
        <property type="gene ID" value="STH351"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH351"/>
    <dbReference type="eggNOG" id="COG3711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078802_0_0_9"/>
    <dbReference type="OMA" id="VMQEILN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.24.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004341">
        <property type="entry name" value="CAT_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036650">
        <property type="entry name" value="CAT_RNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03123">
        <property type="entry name" value="CAT_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01061">
        <property type="entry name" value="CAT_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50151">
        <property type="entry name" value="SSF50151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRD" evidence="1">
        <location>
            <begin position="66"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="PRD" evidence="1">
        <location>
            <begin position="171"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="275" mass="30700" checksum="42086ECB6360FC54" modified="2004-10-11" version="1">MRPLKALNNNVLLAYDPACGQEVVVVGRGLGFRVSGPIRPDDPRIEKVFRLDESRNRKRFLRLTETLSGDVIGVAEEILNLAAARFGPLGEEAHLTLAEHLAATVDRVRKGMPIPNPFLAQIQLLYPEEYALAGQAVEMIASRLGVTLPEEEQGILALHLLGAWRRESPKVMARHQALIRESAAFLAAEAGISWSDDDPRLSRVLLHLRLAIDLIVSGKAETNPVLDRIRSEYPEEFARARRLGDYMAQRLERPVSDDEVGYLALHLLRLKNLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM0</accession>
    <name>Q67SM0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="5">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ppa</name>
        <name evidence="6" type="ordered locus">STH338</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH338"/>
    <dbReference type="EnsemblBacteria" id="BAD39323">
        <property type="protein sequence ID" value="BAD39323"/>
        <property type="gene ID" value="STH338"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH338"/>
    <dbReference type="eggNOG" id="COG0221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073198_1_2_9"/>
    <dbReference type="OMA" id="DEPTFPG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="171" mass="19234" checksum="997341C81647CF9D" modified="2004-10-11" version="1">MSDAVSEALVEAIIEIPAGSQNKYEVDKKRGLLRLDRVLYSPVHYPTDYGFVDETLEEDGDPIDILVLVSNPTVPGCIVDTRIIGVLVMSDDKGVDNKLLGVAQKDPRYAQVADLSGVPPHRLLEIEHFFRTYKELEGKRTVIEGWFGLDVALEKLRAARELYRRTRSAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP5</accession>
    <name>Q67SP5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39298">
        <property type="protein sequence ID" value="BAD39298"/>
        <property type="gene ID" value="STH313"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH313"/>
    <dbReference type="HOGENOM" id="CLU_1453717_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="186" mass="20246" checksum="8B7760BACFD947A3" modified="2004-10-11" version="1">MLHEPWQIWLRPSCFNPHPALGPAATGALLPLQGVHSLVSILTQPTPRATGRASDGGREVLGVSILARPWIRVLPAVTVEASRVTGFQEPGATPRVELNCPLIEHVSILTRTLGRVLRATASYQSEQKWPILTRSRGRVLGNLIRFQTILSLTRMTPQVTLPLFQPSHSLAAGCYGTRGKPPGRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP8</accession>
    <name>Q67SP8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pre-crRNA processing endonuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-C/Dvulg subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH310"/>
    <dbReference type="EnsemblBacteria" id="BAD39295">
        <property type="protein sequence ID" value="BAD39295"/>
        <property type="gene ID" value="STH310"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH310"/>
    <dbReference type="eggNOG" id="ENOG502Z82V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086014_0_0_9"/>
    <dbReference type="OMA" id="YLGCREF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010155">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029950">
        <property type="entry name" value="Cas_CT1134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01876">
        <property type="entry name" value="cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR029950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="26178" checksum="C144FED6B4958227" modified="2004-10-11" version="1">MACFSRPEFKVERVSYPVMTPSAARGILEAIFWRPEFRYQIRAIGVLKPGKTISILRNELAERQGNAPIFIEDQRQQRASLVLRDVAYLIHADMVLRPHATDPIYKYVDQFRRRVERGQYHHAPYLGTREFPAFFSPDNGETPPDLNLDVGPMLFDIAFIPSSQRPEMEFHRHGPGGACRVTGYAQPLFFDARVEHGWLHVPPQKYDDLYRMEGDDAAGTGQGSRPFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ7</accession>
    <name>Q67SQ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194436.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH301"/>
    <dbReference type="EnsemblBacteria" id="BAD39286">
        <property type="protein sequence ID" value="BAD39286"/>
        <property type="gene ID" value="STH301"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH301"/>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049311_3_3_9"/>
    <dbReference type="OMA" id="DPFNPDH"/>
    <dbReference type="OrthoDB" id="1282433at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="243" mass="27549" checksum="A29794B5B89043B2" modified="2004-10-11" version="1">MTGTREMRILTIGAHAADQELSAGMIIAKYTAAGHKAFILSLTPGEKGHPTLSAEEYAEQKRREAAECARKLGAESIVLNYKDAELPYNDEISLEVCDIIRDIKPDVIITHPEHSIHRDHRHAHKIAMDAHFFAALKWIERAKPAHWTRRVYFSENWEDMEDYEPDIYVEIPEPIFEQYCDALSSFALWNGGTGWPYADYYKSLARLRGCLGGGLNWKYAATLMVPKGGLRQYAAGDRLPLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS2</accession>
    <name>Q67SS2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group II intron-encoding maturase variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH286"/>
    <dbReference type="EnsemblBacteria" id="BAD39271">
        <property type="protein sequence ID" value="BAD39271"/>
        <property type="gene ID" value="STH286"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH286"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2412088_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="92" mass="9635" checksum="1D9F85B1BDC21986" modified="2004-10-11" version="1">MERVVARENMLAALKRVERNGGAPGVDGVPTERLRDHEAGGGAGIQEYPRLPGGTVTAQGQRAEECGRPPMAATVPRVQLLQEPGSSHSHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS9</accession>
    <name>Q67SS9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39264">
        <property type="protein sequence ID" value="BAD39264"/>
        <property type="gene ID" value="STH279"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH279"/>
    <dbReference type="HOGENOM" id="CLU_1593715_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="18250" checksum="907712620143F0B5" modified="2004-10-11" version="1">MEIASTSSLFRSVSGAPNRRYSVCIYKSHIWSTRRALKAANPPSNNEIRPTARKMVRVMPRAPCLINGLPSLRRPMTDDNSAKDTVTATKFAFAFSSIPFLRSFSEACNLSNVPRSISTYLSLSGGSSPVSIIGRISSTRRRCATQAPAYGRISSSIMSINLLTNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ST9</accession>
    <name>Q67ST9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39254">
        <property type="protein sequence ID" value="BAD39254"/>
        <property type="gene ID" value="STH269"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH269"/>
    <dbReference type="HOGENOM" id="CLU_2994982_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="57" mass="6576" checksum="A1B8BA61EA359000" modified="2004-10-11" version="1">MPVYARNPREAAEIKRLAESEYLYCVPRGPLAYYVGTDPALIDPLYWYTIQTAQHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU2</accession>
    <name>Q67SU2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39251">
        <property type="protein sequence ID" value="BAD39251"/>
        <property type="gene ID" value="STH266"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH266"/>
    <dbReference type="HOGENOM" id="CLU_2014100_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13381" checksum="E82B0E309F9E9787" modified="2004-10-11" version="1">MLASLAVGVLACLAARRLVTLLSLIVYLAVGGRRTASGTVGRWLALSAGVLAPIGLIPLIWWVLLTDPATWTASQPYHIVLMMVSLGLSLGYFRDRRRPGTVMWCLRQEGLALAARRAGRRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU8</accession>
    <name>Q67SU8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194395.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH260"/>
    <dbReference type="EnsemblBacteria" id="BAD39245">
        <property type="protein sequence ID" value="BAD39245"/>
        <property type="gene ID" value="STH260"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH260"/>
    <dbReference type="eggNOG" id="COG4756">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_822987_0_0_9"/>
    <dbReference type="OMA" id="YYITFAV"/>
    <dbReference type="OrthoDB" id="1127117at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012443">
        <property type="entry name" value="DUF1646"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07854">
        <property type="entry name" value="DUF1646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019205">
        <property type="entry name" value="DUF1646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="348" mass="37033" checksum="2A6E11636EBE9FF4" modified="2004-10-11" version="1">MADTYVIPGLVVILLLVLILPFAFKAIEHQLEIFLFIMGVSAALVSGIMDQHLIMKALEEPIMITAAVLIAGILFKLFQTKLQAGIRAILRVVPFPVFVFLVVTVLGLVSSVITAIIASLVLVEVVGALRLDRKTEVNLVILACFSIGLGAALTPLGEPLATIAVSKMGGDFWYLLRTIGVYVVPTVVALGLLGALFLKGGRPEAAAQAEGPGLIEQEDGESFKDVIVRALKIYLFVMALTFLGDGFKPLIDRYVLGLPSAALYWINMLSAVLDNATLTAAELSPAMAPAQVKAVLMGLLISGGMLIPGNIPNIVSASKLKIGSREWARTGVPLGLALMVIFFFIVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV8</accession>
    <name>Q67SV8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH250</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SecG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH250"/>
    <dbReference type="EnsemblBacteria" id="BAD39235">
        <property type="protein sequence ID" value="BAD39235"/>
        <property type="gene ID" value="STH250"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH250"/>
    <dbReference type="eggNOG" id="COG1314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094156_6_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="58" mass="6122" checksum="5EFF0FC3472E555C" modified="2004-10-11" version="1">MLLQSGKSAGLSGSIAGAGEQIFGKKKGLDEILNRFSMAFATLFVLTSVLMLFLEARG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW0</accession>
    <name>GPMI_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">iPGM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpmI</name>
        <name type="ordered locus">STH248</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194383.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SW0"/>
    <dbReference type="SMR" id="Q67SW0"/>
    <dbReference type="STRING" id="292459.STH248"/>
    <dbReference type="EnsemblBacteria" id="BAD39233">
        <property type="protein sequence ID" value="BAD39233"/>
        <property type="gene ID" value="STH248"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH248"/>
    <dbReference type="eggNOG" id="COG0696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026099_2_0_9"/>
    <dbReference type="OMA" id="FMDGRDT"/>
    <dbReference type="OrthoDB" id="338375at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="PTHR31637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="SSF64158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000212218" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="259"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01038"/>
        </source>
    </evidence>
    <sequence length="517" mass="56812" checksum="0C59E2B0CA053969" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW9</accession>
    <name>Q67SW9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RNA polymerase sigma-54 factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH239</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-54 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194374.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH239"/>
    <dbReference type="EnsemblBacteria" id="BAD39224">
        <property type="protein sequence ID" value="BAD39224"/>
        <property type="gene ID" value="STH239"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH239"/>
    <dbReference type="eggNOG" id="COG1508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020569_1_1_9"/>
    <dbReference type="OMA" id="NPPAIEF"/>
    <dbReference type="OrthoDB" id="1906842at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000394">
        <property type="entry name" value="RNA_pol_sigma_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007046">
        <property type="entry name" value="RNA_pol_sigma_54_core-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007634">
        <property type="entry name" value="RNA_pol_sigma_54_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038709">
        <property type="entry name" value="RpoN_core-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32248">
        <property type="entry name" value="PTHR32248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00309">
        <property type="entry name" value="Sigma54_AID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04963">
        <property type="entry name" value="Sigma54_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04552">
        <property type="entry name" value="Sigma54_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000774">
        <property type="entry name" value="RpoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00045">
        <property type="entry name" value="SIGMA54FCT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02395">
        <property type="entry name" value="rpoN_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00717">
        <property type="entry name" value="SIGMA54_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00718">
        <property type="entry name" value="SIGMA54_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma54_CBD" evidence="11">
        <location>
            <begin position="92"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma54_DBD" evidence="10">
        <location>
            <begin position="293"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="452" mass="50366" checksum="B0179E66F9E77547" modified="2004-10-11" version="1">MRLGFELRMQQTQRLVMTPELRQAITVLQKPVAELSELIAGELLENPCLEVEPREPSEDGAPSEVGRLLDFLNRGGSHGAGAPPEREEDTSFEPFTPRMPTLAEHLHSQLGLLRLDRAQERIARFLIGCIDDHGYLAVSLGEAAAQLGATEEQVEAALKVIQSLDPPGVGARSLQECLLIQWATLDDPNPLVPELIRHHLQDLAAGRIPRIAERLGVDCAAVQAAADAIRNLDPKPGRRFGGGDEARYVVPDVFIERVGQEYVVMVNEAPLPRLSVSTHYRRLLDAADGHTRRYVEERIQSALWLIRSIEQRRLTLLRVTEAIVRFQREFFDRGPRHLRPLTLREVAQAVGVHESTVSRATSGKWAQTPQGMFELKYFFSSGVETGHGEGVAAEAVKRMIADLIRQEDPARPLSDQALAEALAAQGLRIARRTVAKYREEMGLPNSGQRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SZ0</accession>
    <name>Q67SZ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194353.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH218"/>
    <dbReference type="EnsemblBacteria" id="BAD39203">
        <property type="protein sequence ID" value="BAD39203"/>
        <property type="gene ID" value="STH218"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH218"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="CEGYRRP"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="240" mass="26785" checksum="6749F9B3E2AD114F" modified="2004-10-11" version="1">MIELLNVSKSYSGGTKKAVDNLSLTIRPGEVFGFLGPNGAGKTTTIKMIVGLLRPDSGTVRVGGYDVVRQPLEAKRQLAYVPDTPEVYDKLTGLEYLNFIADVYGISPRERQERIAQFADAFDLTHALLDRVDSYSHGMQQKLVLISALMRDPPVWVLDEPMVGLDPRSSYQLKELMAEHARRNRTLFFSTHVLEVAERLCDRVAIINKGRIIACGTLDELRSGQTGETLESIFLELTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T02</accession>
    <name>Q67T02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH206"/>
    <dbReference type="EnsemblBacteria" id="BAD39191">
        <property type="protein sequence ID" value="BAD39191"/>
        <property type="gene ID" value="STH206"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH206"/>
    <dbReference type="HOGENOM" id="CLU_1805184_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269968" evidence="2">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="143" mass="16197" checksum="E2E44851C80A8146" modified="2004-10-11" version="1">MRGLRRWLAIALVAAWACLLLGGTSLAAGSEAEKQAPKLSPGMEQLLKDIRALRQSRLEQLNAEIDQLIDQAHRAGKITDEEAARLREWRAVRRMGLSPHASEAEVKEKLDEAVKNGRLTKEQAKRILKEWQRARRAHHKARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T11</accession>
    <name>Q67T11_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH197"/>
    <dbReference type="EnsemblBacteria" id="BAD39182">
        <property type="protein sequence ID" value="BAD39182"/>
        <property type="gene ID" value="STH197"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH197"/>
    <dbReference type="HOGENOM" id="CLU_2304620_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="100" mass="11000" checksum="422EF6192FCE8D43" modified="2004-10-11" version="1">MEAVRDRVVRSAPGRTGGRISVAVPAASTLRSWEQRVYVPPARNKGRRNKGRHRLYTEADPIPLRPGGRRVVAEAQKAARLRSRTVGRLPTRAVGPLRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T72</accession>
    <name>Q67T72_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Peptide chain release factor 2</fullName>
            <shortName evidence="6">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prfB</name>
        <name evidence="9" type="ordered locus">STH136</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH136"/>
    <dbReference type="EnsemblBacteria" id="BAD39121">
        <property type="protein sequence ID" value="BAD39121"/>
        <property type="gene ID" value="STH136"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH136"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_6_0_9"/>
    <dbReference type="OMA" id="YVFHPYQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="8">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="6">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="308" mass="34818" checksum="FAC2D2E622CFAEB7" modified="2004-10-11" version="1">MEQLESFRRRLDDLEVMIDLAEEASDAEMAAEARAEADALEEQVRQFELQVLLNGKYDAYNAILTIHAGAGGTEAQDWAEMLLRMYVRWAERRGFKVEELDRLDGDEAGIKSVTIAVRGPYAYGFLRPEMGVHRLVRISPFDASGRRHTSFAAVEVMPEIEDDVDDIEIRPEDLRIDTFRSSGAGGQHVNKTESAVRITHLPTGIVVSCQQERSQMQNRAVAMMMLRSKLVELKIEEQNKEMAQIRGPQMEIAWGSQIRSYVFQPYTLVKDHRSGVEVGNAQAVIDGELDPFIYGYLEKRANGELARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG0</accession>
    <name>Q67TG0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6431 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH48</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH48"/>
    <dbReference type="EnsemblBacteria" id="BAD39033">
        <property type="protein sequence ID" value="BAD39033"/>
        <property type="gene ID" value="STH48"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH48"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1320339_0_0_9"/>
    <dbReference type="OMA" id="FRCMLSE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045536">
        <property type="entry name" value="DUF6431"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20020">
        <property type="entry name" value="DUF6431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6431" evidence="1">
        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="24191" checksum="5B4BB9E3C9CF5764" modified="2004-10-11" version="1">MHLCMCILLPLGQNVQTYLRKYGTCGPDLPLHCPGCGGRMRRHGRYWRWVFTAHQKAYIPIYRWWCPGCRKTCAILPDFLKPYARFITLVREAVVRGRVRRGLPWSTLARRLSSPTVSWLSEKTLRRWLVRARALAGEWSQYLAERVLRFWPDTDLDALTPRREGPDAALHFLLDVGDWYRRQMGRRPEEHGGVFAALNRLGEGTASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JI2</accession>
    <name>Q67JI2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3186</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197299.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3186"/>
    <dbReference type="EnsemblBacteria" id="BAD42168">
        <property type="protein sequence ID" value="BAD42168"/>
        <property type="gene ID" value="STH3186"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3186"/>
    <dbReference type="eggNOG" id="COG0801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097916_1_1_9"/>
    <dbReference type="OMA" id="QVILRQN"/>
    <dbReference type="OrthoDB" id="1953248at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="8">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="175" mass="18954" checksum="B9D0707B995D9604" modified="2004-10-11" version="1">MTTAYLSLGANIGDPEGQLVSALRRLASAPGTSLAAVSGLYRTAPQGLTDQPDFLNCAARIETALDPYGLLALTQSIEADLGRVRTIRWGPRTIDIDILLHGQARLDGPGLTLPHPRMLERAFVLAPLLEVWRDGEEPAGISRTALEGRLSDLGDQPVVRIRDRQSLLRQIQGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JM6</accession>
    <name>Q67JM6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197255.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3142"/>
    <dbReference type="EnsemblBacteria" id="BAD42124">
        <property type="protein sequence ID" value="BAD42124"/>
        <property type="gene ID" value="STH3142"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3142"/>
    <dbReference type="eggNOG" id="COG4231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1822054_0_0_9"/>
    <dbReference type="OMA" id="TITEKCI"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="149" mass="16080" checksum="C0E8652F1164B3E7" modified="2004-10-11" version="1">MPHYIDEKCIGCTACVSVCPTEAISGERKQLHYIDPKLCIDCDACVRSCPVLAIADEFGVYKPRIPKRSDWPKPVIDPVSCSGCDFCVDICPFDCLELAGDGPFFGTAVLVRPNACVGCKECEEVCAKGAIIVLAPDEQHPIAVAEGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP0</accession>
    <name>Q67JP0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">radA</name>
        <name evidence="8" type="ordered locus">STH3128</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197241.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3128"/>
    <dbReference type="MEROPS" id="S16.A04"/>
    <dbReference type="EnsemblBacteria" id="BAD42110">
        <property type="protein sequence ID" value="BAD42110"/>
        <property type="gene ID" value="STH3128"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3128"/>
    <dbReference type="eggNOG" id="COG1066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018264_0_1_9"/>
    <dbReference type="OMA" id="FELMRLA"/>
    <dbReference type="OrthoDB" id="505485at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01121">
        <property type="entry name" value="Sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="7">
        <location>
            <begin position="67"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="5">
        <location>
            <begin position="352"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="5">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="456" mass="48679" checksum="BF12310E658E716A" modified="2004-10-11" version="1">MAKSRSAFFCQECGYESPRWMGRCPGCGAWNTMVEEPVNPPGGRGLLTSDPGTGGPPLAIGEVETEDAQRLETGMDELDRVLGGGIVPGSFVLVGGDPGIGKSTLLLQLAAGVARQHGTVLYASGEESARQIRMRADRLGTIHPRLLVLAETNLEAIAHHAAQVAPRLLIVDSIQTVFRPDLPSAPGSVSQVRECAAQLLRVAKGQNLAIFIVGHVTKEGSLAGPRVLEHIVDTVLYFEGDRHASFRILRAVKNRFGSTNEIGIFEMRDAGLAPVGNASQLFLAERPVEVAGSVVMPAMEGTRPLLVEVQALVSASTFVTPRRTAEGIDLNRLQLIIAVLEKRVGLLLGNHDAYVKVAGGVRLAEPAIDLALAVALASSFRDQPPDPRTVVVGEVGLAGEVRAVSRIDQRIREAEKMGFDRMVLPRANLRSLSMKTDVELVGVETVMEGLEAALGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP4</accession>
    <name>Q67JP4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ycf81 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3124"/>
    <dbReference type="EnsemblBacteria" id="BAD42106">
        <property type="protein sequence ID" value="BAD42106"/>
        <property type="gene ID" value="STH3124"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3124"/>
    <dbReference type="eggNOG" id="COG4956">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050839_0_0_9"/>
    <dbReference type="OMA" id="TMPTDLL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="3">
        <location>
            <begin position="296"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="369" mass="39586" checksum="5E9F3B4D4B71A6BC" modified="2004-10-11" version="1">MVLRKNLRIVLTIAGVLSGFGSTFLWRESVALLLSVPEHWGFTLLFFAVGSLVGGLVGFGLYPFVTWLEGRLARTAFIDIILGSVGLISGLLIAALLSVFTGKIPLFGGVISVLSAVIFGYLGMTVTTRKREEVSEAFSSIFSRSARKEHAAGSQAHSKHQEYRGVSRPKILDTSVIIDGRIADIVAAGFLEGPLVVPSVVLEELRHIADSPDVLKRNRGRRGLDILSEIQKLPGADVRIYEGEIDAAEVDIKLLKLAKMLGGVVVTNDFNLNKVAGLQGVRVLNINELANALKPIVLPGEEMTVSVVKDGKEPGQGVGYLDDGTMIVVDGGKRYIGDTIDVLVTSVIQTAAGRMIFAKPKMTVEKVAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP5</accession>
    <name>ISPD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">MEP cytidylyltransferase</fullName>
            <shortName evidence="1">MCT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispD</name>
        <name type="ordered locus">STH3123</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-methyl-D-erythritol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="EC" id="2.7.7.60"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JP5"/>
    <dbReference type="SMR" id="Q67JP5"/>
    <dbReference type="STRING" id="292459.STH3123"/>
    <dbReference type="EnsemblBacteria" id="BAD42105">
        <property type="protein sequence ID" value="BAD42105"/>
        <property type="gene ID" value="STH3123"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3123"/>
    <dbReference type="eggNOG" id="COG1211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061281_2_2_9"/>
    <dbReference type="OMA" id="ERQHSVY"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00453">
        <property type="entry name" value="ispD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000075633" description="2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="1">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="1">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00108"/>
        </source>
    </evidence>
    <sequence length="236" mass="25239" checksum="6FD484898FA73AA8" modified="2004-10-11" version="1">MSIHAGGLQVPVSVIIPAAGSGRRMGGGTAKQFLPLRGEPVLVRTVRRFSECPLVDEIVIAAGDVEATRALVGHMPKVTRIVQGGAERQDSVWAALQAVHSRPRIVAVHDAARPLLTADVLKGVLQAAADHPAQVVAVPVQDTIKQVGPDGVVVATPDRSMLWAVQTPQVFWADVLVRAFRQAIADGFLGTDDASLVERIGVPVRVYRGHPGNLKLTTPTDFRLAELLLEEEARQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR7</accession>
    <name>Q67JR7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Formate dehydrogenase gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH3101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197216.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3101"/>
    <dbReference type="EnsemblBacteria" id="BAD42083">
        <property type="protein sequence ID" value="BAD42083"/>
        <property type="gene ID" value="STH3101"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3101"/>
    <dbReference type="eggNOG" id="COG2864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091368_1_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="3">
        <location>
            <begin position="11"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD42083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="239" mass="26814" checksum="2BC77859B6B4D040" modified="2004-10-11" version="1">MARANGSIKKFSRTQVLFHWLYAGSWIVLALTGSIFLWRGNPAEPAAGLGPLLHGSVGQTLRLVHRIAAIGLMSSPLVWLLGDPKTVWPDLKELFTFTKNDLKYMLIAPLYYTTGKGHLPPQGKFNGGHKVNFYVVFLTYFTFIGSGLVMWFGRGAVSDEAFHAAQFIHSLSFWLGSGLFLLHFYLTAVHPFTRRSLSAMVDGWTELQYARLEHGEWVEREIRNGTAQIRESEQAAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K48</accession>
    <name>Q67K48_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDDEXK_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2967"/>
    <dbReference type="EnsemblBacteria" id="BAD41950">
        <property type="protein sequence ID" value="BAD41950"/>
        <property type="gene ID" value="STH2967"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2967"/>
    <dbReference type="eggNOG" id="COG2887">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070318_0_0_9"/>
    <dbReference type="OMA" id="NWQTRLY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDDEXK_1" evidence="2">
        <location>
            <begin position="83"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="33202" checksum="CF2FC85C7FD1706B" modified="2004-10-11" version="1">MTGSVAQPIPLHERHFSALALRLYAECPLRFRRRYVDGLAIPGLPDDPEERRAMLQGERFHLMARRYYSGLEPGTLGAPADRAELEDWMQKLVRYLPRRPGYAYYPELELRLSEPALRLVARFDLVVVAPDGEATIYDWKTLRHMPPRAKLEAAFQTTVYRYVLCAAGGAYAPGGAFDPDRVSMVYWNPLRGGREERLPYSAARFREDGRRLRQLVAQILATPPDGFPPTGDPRLCARCEYRQLCHGPLADRPLPEPGDGWPEAALEPGQEVPVEDEEPAGAFDADPDGLAGLPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K53</accession>
    <name>Q67K53_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2962"/>
    <dbReference type="EnsemblBacteria" id="BAD41945">
        <property type="protein sequence ID" value="BAD41945"/>
        <property type="gene ID" value="STH2962"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2962"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="LKAWIMK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="22939" checksum="10ADF8E20EE3FEBB" modified="2004-10-11" version="1">MPTDEELVAAYLKGDEAAFEEIVRRYEDRLYRLSYRMLGNHHDALDAVQEILVKLMAALPKFEGRSRFGTWVYRLAANTCLDIRRKRGRTAAESLEATLEFSPAASLAILDDEPADNPDLYLEQQYRENVVRAALDKLPESQRRLLELRDLEDFSNSQVADMLGIEVGALKARLHRARQALKRVLDAGVYVPGYTSPAATDLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K97</accession>
    <name>MURC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murC</name>
        <name type="ordered locus">STH2918</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67K97"/>
    <dbReference type="SMR" id="Q67K97"/>
    <dbReference type="STRING" id="292459.STH2918"/>
    <dbReference type="EnsemblBacteria" id="BAD41901">
        <property type="protein sequence ID" value="BAD41901"/>
        <property type="gene ID" value="STH2918"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2918"/>
    <dbReference type="eggNOG" id="COG0773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028104_2_2_9"/>
    <dbReference type="OMA" id="DITYQLR"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000182172" description="UDP-N-acetylmuramate--L-alanine ligase">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="111"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <sequence length="482" mass="52227" checksum="A63AE4C4520A6FC8" modified="2004-10-11" version="1">MLKARKVHFIGVGGYGMSALAMVLKQRGTAVSGSDVAASARTQRLAEAGVEVHIGAHDAAHIEGADVVVYSTQVPEDNPELAAARARGLRVLHRSELLAEFLNGRSIAVAGTHGKTTTSSMVACLLEKAGLDPTILLGGDLEVIGGTGKAGRGDWVVAEADESDRSFLRYHPRVAVVTNVEPEHLEFWEGSFARIQEGFRQFLRQVQPGGLAALCADDPTLRSIGQELRNQPGVVHVVFYGTHPEAEWRAENIRPWEKGIVYDCVRDGRLLGEVRLPIPGRHNALNSLGALVAGDYAGLSFRQMQETLLSFGNARRRFEVWADVDGIRVVDDYAHHPTEIRATLAAAREQAGRRVVAVFQPQRYSRTHWLMDEFATAFQDADVLVLTRIYSPPGERPIPGVSAEALARRIQANTGRPVAVISDQEEILRFLLAEVRPGDTVITMGAGDIWKVARALAQALQSRAVTRTASSGSGRPAPGSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA1</accession>
    <name>Q67KA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stage V sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoVE</name>
        <name evidence="8" type="ordered locus">STH2914</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197031.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2914"/>
    <dbReference type="EnsemblBacteria" id="BAD41897">
        <property type="protein sequence ID" value="BAD41897"/>
        <property type="gene ID" value="STH2914"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2914"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_0_1_9"/>
    <dbReference type="OrthoDB" id="1133883at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="404" mass="43174" checksum="5B98AA11D177A097" modified="2004-10-11" version="1">MARTVPSARRPPAPAAPPPVPGLPDGLLIVALIGLVSLGMVMIYSTTVHEGSPSAVVRELALQFGVGCAGLLLGMLVPLSWWRRLTPLLLVGAAGALASLLIPGNPLAITRLGATRWLQVGPLSIQPSEFAKLAFILFSAGFLDRNFRRMRLPQWMVYLGVTAGVALLIYREPDLGTAAVIGGIAICMLWVARVHWFWVLSLFGGAVGAILLLARTKQHQQERLLAWRNPWAFQDTIGHQIIQSWTAMARGGLWGVGLGQSLQKLGNRLPEAETDFIFSVVVEELGLVGGIAVILLFVLFAWRGFTIALRAPDRYSMLLAAGITTWVAGQAALNVGVVTGTLPNTGIPLPFLSSGGSSLLALMIATGLLLAVSRLPPAEPKPSARAEPGPEPAARPGRRRFRHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KB9</accession>
    <name>Q67KB9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative hydrolase of the HAD superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2896"/>
    <dbReference type="EnsemblBacteria" id="BAD41879">
        <property type="protein sequence ID" value="BAD41879"/>
        <property type="gene ID" value="STH2896"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2896"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_2_9"/>
    <dbReference type="OMA" id="VMIGINP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02240">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044266">
        <property type="entry name" value="PSP_YsaA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="28430" checksum="43A85DD8E79E7F8F" modified="2004-10-11" version="1">MRAVLFDLDETLILDEPVTREALAAAAALAGSRADPERLAARAWAEARRRWAEGPYAAYCRRIGHSAAEGLWARYDRGDHPAIQGLRQWAPDYRVAVWREALAEQGVRDDALAEAMAERFAAARRRYLRYPEVDPLLAALRERGYLLGIVTNGVPDLQREKLAVSGLEREVDAVVISGEVDCGKPDPGIFRHICRSLGVAPHEAVMVGDNPGRDVAGGRAAGLLTVWVQRGGRERDERHPADLACTDLSALLPWLEALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC8</accession>
    <name>Q67KC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin converting factor small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197004.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2887"/>
    <dbReference type="EnsemblBacteria" id="BAD41870">
        <property type="protein sequence ID" value="BAD41870"/>
        <property type="gene ID" value="STH2887"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2887"/>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_1_2_9"/>
    <dbReference type="OrthoDB" id="1995573at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010038">
        <property type="entry name" value="MoaD_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01687">
        <property type="entry name" value="moaD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="92" mass="9767" checksum="BD39717EA01607D2" modified="2004-10-11" version="1">MVVKLFGNLRLHGGGSRVEVDLGGGRTVADLLDRLFALHPALRPHVVTPEGDALLPHVNIMLNGRLIRDLQGLQTPVSEQDSVAIFPPSAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KF6</accession>
    <name>Q67KF6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ordered locus">STH2857</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2857"/>
    <dbReference type="EnsemblBacteria" id="BAD41842">
        <property type="protein sequence ID" value="BAD41842"/>
        <property type="gene ID" value="STH2857"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2857"/>
    <dbReference type="eggNOG" id="COG0152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045637_0_2_9"/>
    <dbReference type="OMA" id="TKFEFGF"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="9"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="33168" checksum="32317E1F5BA4A849" modified="2004-10-11" version="1">MPDPGLRRLHRGKVREMFELDADRLLMVATDRLSAFDVVFPQGIPGKGRVLTQLSALWFGATRHIVANHLVSDDLGGLGLPGDVRRLLEGRSLVVRRARRIDVECVVRGYLAGSGWKEYQASGTVCGIPLPAGLRLSSRLPEPIFTPALKNDRGHDENVPEAEVRRRLGDAVTDFIKEKSLALYRYAAGVAEKAGMILADTKFEFGFVGDEIVLIDEAFTPDSSRYWPADRYREGEPVDSLDKQPVRDWAERTGWNKQPPAPELPGELIEETARRYADVLDRLRKVLAETAAGAGGGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN9</accession>
    <name>NUOD2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit D 2</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit D 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit D 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoD2</name>
        <name type="ordered locus">STH2774</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KN9"/>
    <dbReference type="SMR" id="Q67KN9"/>
    <dbReference type="STRING" id="292459.STH2774"/>
    <dbReference type="EnsemblBacteria" id="BAD41759">
        <property type="protein sequence ID" value="BAD41759"/>
        <property type="gene ID" value="STH2774"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2774"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_9"/>
    <dbReference type="OMA" id="IMGTSME"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000357944" description="NADH-quinone oxidoreductase subunit D 2">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01358"/>
        </source>
    </evidence>
    <sequence length="384" mass="43902" checksum="9309D348E8B93F69" modified="2004-10-11" version="1">MNVERTQELLVNMGPQHPSTHGVLRLMIKLDGEQVTWCEPDIGYLHRCFEKLAEQKTYPQVIPFTDRTDYLAAMLNELCYVEAVEKLFGDAIQVPERAQYIRVMLAELQRITSHLLALGSMAMDLGATTPFLYCWRDREKLYSLFERITGGRMLYNYLRIGGVRNDLPEGILGTPQDGEDKADKTIWGFINYFDSYVYPEWKALVTGNRIFQYRTKNIGVLTAEQAIAYSCSGAVLRGSGVKWDLRKNLPYAIYDRFEFDIPVGQNGDSFDRWWVRQEEMYQSSRIVKQCLEWLAENPGPVMAPKMPRVLKPPKGEVYHRIEGARGEVACYVVSDGSTNPYKVKWRSPAFTHLQLMPLLCPGHKIADIIAILGSIDVVLGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ9</accession>
    <name>Q67KQ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional repressor of xylose-utilizing enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2753"/>
    <dbReference type="EnsemblBacteria" id="BAD41738">
        <property type="protein sequence ID" value="BAD41738"/>
        <property type="gene ID" value="STH2753"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2753"/>
    <dbReference type="eggNOG" id="COG1940">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036604_13_2_9"/>
    <dbReference type="OMA" id="DFVLLWN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="HTH marR-type" evidence="4">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="391" mass="41386" checksum="BF74B2A232B7C458" modified="2004-10-11" version="1">MPRPANARLMKQLNRQAVLSAIRDRGPISRVELAALTGLSQPAVTAIVRDLLDLGLVEEKGLGQSSGGRPPIMLLFNPAARFVLAVCLEGERLWGGLADLSGALQVEAEGVLSPDPHDPARSVCDFLQGLIDRSGVEASRLAAVAVGVPGIAHTEGTVSHAPSLGWWQEVPLRDRLRQCFGVPVVVENDVNLMALGEYFQGAGVGVANLALMHVSEGIGAGILIDGNLFRGARNAAGEVGYLPLGPLSPRRPQDFGLFELHYSSRAIRQQLQAALPEAPGDDGRPVRRLRELAEAGLPWARSLFGDALRHWAHAVASITCILNPELVLLAGDAVDCGREGLSEVNRIVADLVPDPPEIRFAVLGSRAALTGAVAMALSLADENAYGIEADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY4</accession>
    <name>Q67KY4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196799.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2677"/>
    <dbReference type="EnsemblBacteria" id="BAD41662">
        <property type="protein sequence ID" value="BAD41662"/>
        <property type="gene ID" value="STH2677"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2677"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="NAKAWLF"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="131"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="22515" checksum="B587280868C2F9D8" modified="2004-10-11" version="1">MLSLTDEQLVERAQEGDRCALGELISRYEQRTYNLAYRLMGNHADACDAAQEALVRVCVRLHNFRGDSQFSTWLYRVVTNTCLDELRRRGRTRCASLDEAMPLDEGSVPRQAVDDSDGPVEYAERHEVQAAVQRAIGRLPYDYRVVVILRDLHNLSYQEIATVLGTTLGTIKSRLHRARQALRCIIEDTEAHRLDIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L02</accession>
    <name>Q67L02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Small acid-soluble spore protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">SASP are bound to spore DNA. They are double-stranded DNA-binding proteins that cause DNA to change to an a-like conformation. They protect the DNA backbone from chemical and enzymatic cleavage and are thus involved in dormant spore's high resistance to UV light.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha/beta-type SASP family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2659"/>
    <dbReference type="EnsemblBacteria" id="BAD41644">
        <property type="protein sequence ID" value="BAD41644"/>
        <property type="gene ID" value="STH2659"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2659"/>
    <dbReference type="HOGENOM" id="CLU_2132275_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001448">
        <property type="entry name" value="SASP_alpha/beta-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018126">
        <property type="entry name" value="SASP_alpha/beta-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038300">
        <property type="entry name" value="SASP_sf_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00269">
        <property type="entry name" value="SASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00304">
        <property type="entry name" value="SASP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="12149" checksum="450110E31743EF8D" modified="2004-10-11" version="1">MCIFGFTGRDSFPFVYEIASAAKIGAGGSHMQKKTKSPTAKGRQKKPKELTPLDRLKLEIAQELGLAEKIARSGWAELTAAESGRLGGILNRRLKELKLAIGPKGTLIPTAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L12</accession>
    <name>Q67L12_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2649"/>
    <dbReference type="EnsemblBacteria" id="BAD41634">
        <property type="protein sequence ID" value="BAD41634"/>
        <property type="gene ID" value="STH2649"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2649"/>
    <dbReference type="HOGENOM" id="CLU_157752_0_1_9"/>
    <dbReference type="OMA" id="TIGIYQI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020390">
        <property type="entry name" value="Uncharacterised_YqhV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10942">
        <property type="entry name" value="DUF2619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="88" mass="9366" checksum="0A173936D501186A" modified="2004-10-11" version="1">MPVEERLIWGMRLARMLSACVEVCVALMLLRMADPKAMLRLNALAGLVGPAVFIAVSALGLAASLGRLEPGRLLVVLLGIALVVWGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L59</accession>
    <name>Q67L59_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative formate dehydrogenase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2602</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2602"/>
    <dbReference type="EnsemblBacteria" id="BAD41587">
        <property type="protein sequence ID" value="BAD41587"/>
        <property type="gene ID" value="STH2602"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2602"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061371_1_0_9"/>
    <dbReference type="OMA" id="AGSNEWK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="4">
        <location>
            <begin position="60"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="23591" checksum="91F6DF8A0A45634A" modified="2004-10-11" version="1">MGAMCPAIGHTELRMGVESLALTRRDLLKSSLAAGGALSLLGLGGKAEAKERDDLKIEGAKDYRTICCYCSVGCGIQLWVKDGEVVHVNGDPDHPINEGTLCPKGASIANLRIVADKNNQYQPNPRRLTKVLYRAPGATDWEEKDWDWAIERIARNVKAARDATFERVDENGVTVNRTFAMAHIGSAALDNEENYVLAKLMRGLGIVRLEHHARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA1</accession>
    <name>Q67LA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2560"/>
    <dbReference type="EnsemblBacteria" id="BAD41545">
        <property type="protein sequence ID" value="BAD41545"/>
        <property type="gene ID" value="STH2560"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2560"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_4_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="188" mass="19898" checksum="E09CCDC27D1F5C2D" modified="2004-10-11" version="1">MVQGPTPTAGGNRMPLSEIDTERLLLRPVGPGDAPDLQALAAAEAGEPPDPAGAPFPTADVERWIAEQQAGWQAGTHCTFAARTRAGAFVGVVQLRRSPSAPRAELGFATAPGARRQGYATEACRAVLRFAFEHLRLARVSALCLETNRASVRVLEKLGFRLAARLEPEGAQGAGAAPLLFYVRTCDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD2</accession>
    <name>Q67LD2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196652.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2529"/>
    <dbReference type="EnsemblBacteria" id="BAD41514">
        <property type="protein sequence ID" value="BAD41514"/>
        <property type="gene ID" value="STH2529"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2529"/>
    <dbReference type="eggNOG" id="COG0846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060310_0_0_9"/>
    <dbReference type="OMA" id="YCCYISS"/>
    <dbReference type="OrthoDB" id="503036at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039444">
        <property type="entry name" value="SIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13289">
        <property type="entry name" value="SIR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="318" mass="35081" checksum="B11BC88A0E5C64E4" modified="2004-10-11" version="1">MDLYLFGAGASAAEGAPATRDLFPMAYRALGPRFDPQVEEVWEFLGAVFQRPVTGPDTFRYLPALDEVISLVDWSLHVDQGLGPDYDPPRLYRIRQALEHLVCSVLERARGRQRPDDGPHARFARALASRAPDSYALVSLNYDTLLDDALRAAGLTPDYALDPGKSGGPLLLKLHGSLNWVHCPACDQIAVLREPVAHQLTRAAGLACGRCGSRRLAGVVISPTWLKRYSPGRLTRVFEQALEAVRRARRIVIIGYSLPPADVAVHHLLRRGLLTRAHPEPPAVEVITRSPSEQLLDRFRRLFGPGVAFDCTGFRGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LG3</accession>
    <name>Q67LG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative beta-N-acetylglucosaminidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2498</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2498"/>
    <dbReference type="EnsemblBacteria" id="BAD41483">
        <property type="protein sequence ID" value="BAD41483"/>
        <property type="gene ID" value="STH2498"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2498"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008392_5_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269892" evidence="3">
        <location>
            <begin position="50"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_3" evidence="4">
        <location>
            <begin position="96"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="637" mass="65092" checksum="2AD1500609D2CDDE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH0</accession>
    <name>Q67LH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2491"/>
    <dbReference type="EnsemblBacteria" id="BAD41476">
        <property type="protein sequence ID" value="BAD41476"/>
        <property type="gene ID" value="STH2491"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2491"/>
    <dbReference type="eggNOG" id="COG3835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043769_1_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008599">
        <property type="entry name" value="Diacid_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025736">
        <property type="entry name" value="PucR_C-HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042070">
        <property type="entry name" value="PucR_C-HTH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05651">
        <property type="entry name" value="Diacid_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13556">
        <property type="entry name" value="HTH_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diacid_rec" evidence="1">
        <location>
            <begin position="28"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_30" evidence="2">
        <location>
            <begin position="334"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="405" mass="43975" checksum="AD27C8DE1CF7D1B7" modified="2004-10-11" version="1">MPVALPAAGSQAGRRALPRWWEGITLRITSELAQPIADRAVTILQRNVNIMDEDGVIVASGDPTRIGSFHQAAAQVIRTNRRLDIYPGDEERWVGVRPGVNLPIQVDGRTVGVVGITGPPDQVGPFGELIREMVSLALMQRRAEELERVRLMARESFLRTLLTSKGPIGERMVQDAPLYDLSPDSAYQVLLCQGPRGTDGLSRIWAESEWLAQRLQRGGTDRITFAGPWNGLGVVIATDPPRDLSAVLLEALPKGFAVAGGLIGRGLVGLRRSYETALQSFAAGRLHGNPVALSAYRLRLSRLLTTLPPSEVAEFVAGMLGGLPAADSREGRLLRETVLAYAEEGLNSTGAADRLGIHRHTLVHRLGAIAERSGTDPRSWEGLLSLYLAIQLERLFGQSVEYGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI0</accession>
    <name>Q67LI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196604.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2481"/>
    <dbReference type="EnsemblBacteria" id="BAD41466">
        <property type="protein sequence ID" value="BAD41466"/>
        <property type="gene ID" value="STH2481"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2481"/>
    <dbReference type="HOGENOM" id="CLU_1282672_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="23875" checksum="8F1D5C020CEED8E5" modified="2004-10-11" version="1">MQNAEVRELVQRSLERYAGDERVLDAFWQWASNPTPPSAGYALEENAGFIATNLMLRYGEPAVDLVMSVNEECRRLAGRASIQELQRTIVTVLNGPVGEQLRQGIVRRLSQPTVARRALTAWLVNRARWRRMPGREPVVAGEFDWSLGADLEGSGADCQSAVLRALYGPEVAQVDLAREAMAVGVVNRLFYRNVAGRMEAKLRPGPRLPVSALVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK9</accession>
    <name>Q67LK9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore germination protein KA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gerXA</name>
        <name evidence="3" type="ordered locus">STH2452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2452"/>
    <dbReference type="EnsemblBacteria" id="BAD41437">
        <property type="protein sequence ID" value="BAD41437"/>
        <property type="gene ID" value="STH2452"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2452"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021639_4_1_9"/>
    <dbReference type="OMA" id="TKGWEHR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004995">
        <property type="entry name" value="Spore_Ger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03323">
        <property type="entry name" value="GerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005690">
        <property type="entry name" value="GerBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="476"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="567" mass="62281" checksum="F70BA703DE45787D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL3</accession>
    <name>Q67LL3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="15">PTS system N-acetylglucosamine-specific enzyme IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">STH2448</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196571.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2448"/>
    <dbReference type="EnsemblBacteria" id="BAD41433">
        <property type="protein sequence ID" value="BAD41433"/>
        <property type="gene ID" value="STH2448"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2448"/>
    <dbReference type="eggNOG" id="COG1263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012312_1_0_9"/>
    <dbReference type="OMA" id="AWAFNRF"/>
    <dbReference type="OrthoDB" id="2035550at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00212">
        <property type="entry name" value="PTS_IIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036878">
        <property type="entry name" value="Glu_permease_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018113">
        <property type="entry name" value="PTrfase_EIIB_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013013">
        <property type="entry name" value="PTS_EIIC_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001996">
        <property type="entry name" value="PTS_IIB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00367">
        <property type="entry name" value="PTS_EIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55604">
        <property type="entry name" value="SSF55604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00826">
        <property type="entry name" value="EIIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51098">
        <property type="entry name" value="PTS_EIIB_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01035">
        <property type="entry name" value="PTS_EIIB_TYPE_1_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51103">
        <property type="entry name" value="PTS_EIIC_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-1" evidence="14">
        <location>
            <begin position="2"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-1" evidence="13">
        <location>
            <begin position="397"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate; for EIIB activity" evidence="11">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD41433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="492" mass="51118" checksum="A3B0528E7AB90652" modified="2004-10-11" version="1">MREKAAFFQRASASLMVPLTFVPLPAVLVALGTVLGIGPLESAGLELLRVWLPLFFAMGIAVGFAQAEGMAVLSAAAGYLTMMAVAEGVAGDPAVNFGALGGVAVGAAAVWLYRYAAKVRLPEFLALFSGRRLGPILAALAGVVLGWGFGLVWPGFHRAVVLLGEWIYAAGGAGAFVYGGIIRMLIPTGLHHLLMQLMDYQMGAWTDPATGQVVMGEYIRFLHGDPSAGRLLSGFFLTLAFGPLGAALAMVHEARPEQRRRVSGMMLTGVLTAMVLGITEPIEFAFIFASPLLFGIHVLFSAAASLLGYVLDIHLGGYALPLAVVNWHRQQNGWLLLPLGAAWTALYYVTFRLIIRWRRPPVLGQTDAVPVAGAGGSDARAAAGEPSEAARPAGAAGPSAAAILDALGGPANVRSLTACMTRLRLEVNDPAAVDDAALRAFGAAGVVRSGSSVQVVMGARAGDIEQALQAELAEAAGQGGAGQTAGRQEPCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS3</accession>
    <name>Q67LS3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2388"/>
    <dbReference type="EnsemblBacteria" id="BAD41373">
        <property type="protein sequence ID" value="BAD41373"/>
        <property type="gene ID" value="STH2388"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2388"/>
    <dbReference type="eggNOG" id="COG3881">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132076_0_0_9"/>
    <dbReference type="OMA" id="DFIVADC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="83"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17125" checksum="AFA9F28A37DCD80F" modified="2004-10-11" version="1">MSRARDLIGLSVLASPDMRPVGRVQEVLISRDGRRIRGLVLETGGLLSRRRVLDYQGVKAVGSTYVLAEERYLEDEAETRCGSALTGLPVLDGSGEELGMLDDLHFEPSTGEIHALQLSRGFVDDLLSGKAIVPLNGPAVTGEGAILLDAVAEREGGLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT6</accession>
    <name>Q67LT6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH2375</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196498.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2375"/>
    <dbReference type="EnsemblBacteria" id="BAD41360">
        <property type="protein sequence ID" value="BAD41360"/>
        <property type="gene ID" value="STH2375"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2375"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_647089_0_0_9"/>
    <dbReference type="OMA" id="QMERPIA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269261" evidence="10">
        <location>
            <begin position="34"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S11" evidence="11">
        <location>
            <begin position="39"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="6">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD41360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="424" mass="46210" checksum="7DF6FA1F594F9862" modified="2004-10-11" version="1">MPSHREEIALKRLRAIIACALLLTLLLRLPASADDTWYETSAESGILMDCATGQVLWEKNPDERLAIASTTKIMTALLAIESGRLDETVTIPPEAELIEGSKVYLTQGTTYTLRELLEALILESANDAAVAIAIFLGGSEEAFVRQMNEKARALGLTDTQFRNPHGLDEDGHYSSARDLARLGRVAMADPVYREIAGTEWLTFPWPEMETTREIYNRNLLIQRMPEATGVKNGYTEAALFTLVGSAAADGREVIGVLLGNPDKEQMYADMERLLRHGLYDFSLTTVLSAGDLPGAGGRLEEPLRAWLREGDEVEIAYRRPLPGEEWPEGTVAVVQALVDGRVIAHQPVLAPVTASAQAEPPAHSDGGSGKPPGAGAAAWYQRAGVWMATGAALCGAVIATDRLQARRRQRRFRRHSADRLSASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LV3</accession>
    <name>Q67LV3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196481.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2358"/>
    <dbReference type="EnsemblBacteria" id="BAD41343">
        <property type="protein sequence ID" value="BAD41343"/>
        <property type="gene ID" value="STH2358"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2358"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="NIERAFT"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="240" mass="26350" checksum="4E878B4063EF788B" modified="2004-10-11" version="1">MAAILELRGVDTFYGPVQVHFGVSLTVREGEITCLLGGNASGKSTAMKVILGLVRPRGGQVLLGGEDATRWPTPRRIRWGLGSVPEARRLFPHMTVRENLLMGAYARTDREQVRADYEMVLELFPRVRERLSQVAGTLSGGEQQMVAMARALMGRPRLLCMDEPTMGLSPLWVDRVLELIRQVNARGTTVFMVEQNARAALQIAHRGYVLQNGVVRLEGTAAELAGNPLVEQAYLGGRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ8</accession>
    <name>Q67LZ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2313</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2313"/>
    <dbReference type="EnsemblBacteria" id="BAD41298">
        <property type="protein sequence ID" value="BAD41298"/>
        <property type="gene ID" value="STH2313"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2313"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_5_3_9"/>
    <dbReference type="OMA" id="VHMRESA"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="146"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="348" mass="36059" checksum="61F5E582A608D865" modified="2004-10-11" version="1">MAVIDPRTPAVGGPPSPAQGAPPVAPGVMMQGAQAHPAQVGPLEGAQPPPAAGHPGRGVLPPPGGDPVMSGRSPGILRRLLRHRAAVLGGTIVLLIVLMALLADVIAPYDPIRGRLSDMLQPPSRAHWMGTDEQGRDILSRIIHGSRISLQVGLVAVGISLTTGTIIGAVSAYFGGWLDLLIMRVIDILLAFPSILLAIAITAILGPSLTNAMVAVGIVGMPVYARLVRASVLSLKEQDFVQAARAAGGGHGRILWRHILPNALAPLIVQSTLSIGTAMLDAAGLSFLGLGAQPPAPEWGAMLSGAQVYIQLAPWVVTFPGLAIMLAVLGFNLLGDGLRDVLDPRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M12</accession>
    <name>Q67M12_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DeoR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2298</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2298"/>
    <dbReference type="EnsemblBacteria" id="BAD41283">
        <property type="protein sequence ID" value="BAD41283"/>
        <property type="gene ID" value="STH2298"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2298"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060699_1_4_9"/>
    <dbReference type="OMA" id="VNIFSIE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="27347" checksum="C619C6C584D6BFF6" modified="2004-10-11" version="1">MCDMNIQERLDQILKHLQQHGQVQVRDLAQQFGVSEMTIRRDLERLAREGHLVRTYGGATAAAGLIGEQPFAAKAVSHIEEKERIARAAADLVQDGDVVLLDAGSTTLAISRCLRGRKGLTVITVDLKIALELCDEPGIEVIVTGGTAMPEIYSLLGPVAEQFLRGLTVNIAFLGSSAVDVDFGLTTPTLSKVPLKRAMIGAAQHAVLVADSSKFNRRATYQICPLSSLSRVITDDGLPPAAAAAIRKGGITLDLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M85</accession>
    <name>Q67M85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196348.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2223"/>
    <dbReference type="EnsemblBacteria" id="BAD41208">
        <property type="protein sequence ID" value="BAD41208"/>
        <property type="gene ID" value="STH2223"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2223"/>
    <dbReference type="eggNOG" id="COG4924">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035709_1_0_9"/>
    <dbReference type="OMA" id="DELSNWC"/>
    <dbReference type="OrthoDB" id="418702at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013495">
        <property type="entry name" value="CHP02679"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024466">
        <property type="entry name" value="CHP02679_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024465">
        <property type="entry name" value="DUF2399"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09664">
        <property type="entry name" value="DUF2399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11796">
        <property type="entry name" value="DUF3323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02679">
        <property type="entry name" value="TIGR02679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3323" evidence="2">
        <location>
            <begin position="32"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2399" evidence="1">
        <location>
            <begin position="272"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="435" mass="46615" checksum="A449E317596669E7" modified="2004-10-11" version="1">MSAREAFAAMREAGLEPVLAAARQKVYEADGLRGRVRLTLGAAQLDELANLLGRVSVRDGQVLVPLQDLDAALTRSRFRTGLLDVLEAGYGPIVTRRQEREAAASRWASWLKQVGDALPPVDGVRAWFERVVAGESPSARWVRRVYRTDAEQAARAVAAVGTALARLPVDRGEHELLAVFAASLAGDPHAFDEKEPAGALLEHALRERFGPPPEELRPHEARAFLLDQAGLGADQVSSTVLVAHLAGARWAPAPGASHPMVAAMTACTGAWAVTLGELRRWSAARAHRGRAYVVENPPVFEWLLRRLAGAPPERRATLICTGGFLSAAGFRLLDLLAAEGTEIWYGGDFDRSGITIAVGLAARYGGLFRLWRFGPEDYRAAALGPGGQPLSEADRAALLSVTGPLAPTARAVATGGVTAYQERLVEVLAQDLMEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M87</accession>
    <name>Q67M87_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2221</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196346.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2221"/>
    <dbReference type="EnsemblBacteria" id="BAD41206">
        <property type="protein sequence ID" value="BAD41206"/>
        <property type="gene ID" value="STH2221"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2221"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2398516_0_0_9"/>
    <dbReference type="OrthoDB" id="1899767at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="93" mass="9358" checksum="07E9AD89EFFB05D8" modified="2004-10-11" version="1">MLVLGIPLGGFLAYQLSGEKPAANTGSGLSATSPLTAFRGGFLVLFGARMAGGCTTGHVLSGMAQLSIAGFLFAAAVFAGGIPTALLLKRRWS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-07" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MD5</accession>
    <name>ADDA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent helicase/nuclease subunit A</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent helicase/nuclease AddA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">addA</name>
        <name type="ordered locus">STH2173</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -&gt; 5' nuclease activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of AddA and AddB/RexB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. AddA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67MD5"/>
    <dbReference type="SMR" id="Q67MD5"/>
    <dbReference type="STRING" id="292459.STH2173"/>
    <dbReference type="EnsemblBacteria" id="BAD41158">
        <property type="protein sequence ID" value="BAD41158"/>
        <property type="gene ID" value="STH2173"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2173"/>
    <dbReference type="eggNOG" id="COG1074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001114_3_1_9"/>
    <dbReference type="OMA" id="KQSIYRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01451">
        <property type="entry name" value="AddA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014152">
        <property type="entry name" value="DNA_helicase_suAddA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF48">
        <property type="entry name" value="PTHR11070:SF48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000379358" description="ATP-dependent helicase/nuclease subunit A">
        <location>
            <begin position="1"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="1">
        <location>
            <begin position="553"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1035"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1141"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1371" mass="148869" checksum="0340FC02558E0E3C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ4</accession>
    <name>Q67MJ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41099">
        <property type="protein sequence ID" value="BAD41099"/>
        <property type="gene ID" value="STH2114"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2114"/>
    <dbReference type="eggNOG" id="COG4219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1383559_0_0_9"/>
    <dbReference type="OrthoDB" id="1225707at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="197" mass="21049" checksum="E8BCE53585B95A57" modified="2004-10-11" version="1">MAALTTVVLAGCAATGAVAERQAEQKQDVAAERVATDGGGAAPFRDGQSVLAEDREEGRPSEEESGLDGESAVRAVVEEFGRRLKSVSLSAPDEVVARSLPEQYGDLVSPELLERWQHNPSGAPGRVVSSPWPDHIEIQSVTLRADGAYEVEGWIVEMTSVELVKGGYAAKRPVTLVVARTDEGWRITDFALGEYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK4</accession>
    <name>Q67MK4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Spore germination protein KC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gerKC2</name>
        <name evidence="5" type="ordered locus">STH2104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKC lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2104"/>
    <dbReference type="EnsemblBacteria" id="BAD41089">
        <property type="protein sequence ID" value="BAD41089"/>
        <property type="gene ID" value="STH2104"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2104"/>
    <dbReference type="eggNOG" id="ENOG502Z9N7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051140_0_1_9"/>
    <dbReference type="OMA" id="NGWITPI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008844">
        <property type="entry name" value="Spore_GerAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038501">
        <property type="entry name" value="Spore_GerAC_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35789">
        <property type="entry name" value="PTHR35789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05504">
        <property type="entry name" value="Spore_GerAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02887">
        <property type="entry name" value="spore_ger_x_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269273" evidence="4">
        <location>
            <begin position="22"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="401" mass="44488" checksum="F9E9E609E67E460B" modified="2004-10-11" version="1">MRRRMRLLALSVLLALGSALLSGCWSRVELNDIGLVLGLAVDVGEEEPVRVTLYVPRPLSPEQGGGIGGDQEPIWVVAREADNFSDALALIRLASARRLVFHHLRVVLIGEEYARKHGIGDVLDVLATNHEIRLTVRPFLVEGRAQEVLETLPQLRALQPFNLTGILQTKGGLEWRLKDVLVARASDTHSVWMPTIQVVPRPAITANSPPTAVTLSGVALFRRDYLQRILEPAAYQVIAWFLGNPSGFTITAPCPTGGNGSVSAQVVSGRTRVRPRWQDGGIAFKVEITSNVNIMRSECEMAEVKHAEVREHLEQVLASDLRERIAQFIRITQEAVTDPVGFGKHAQLAFPRFYKTMEDKWGENFWPNTPVEVAVKMTVDQAGLVTGPVHPTERELRERVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ2</accession>
    <name>Q67MQ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Nitrate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">narB</name>
        <name evidence="14" type="ordered locus">STH2056</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2056"/>
    <dbReference type="EnsemblBacteria" id="BAD41041">
        <property type="protein sequence ID" value="BAD41041"/>
        <property type="gene ID" value="STH2056"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2056"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_9"/>
    <dbReference type="OMA" id="GMNAHQH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02791">
        <property type="entry name" value="MopB_CT_Nitrate-R-NapA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041957">
        <property type="entry name" value="CT_Nitrate-R-NapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="10" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269549" evidence="12">
        <location>
            <begin position="29"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD41041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="734" mass="79821" checksum="AC555A18C924086E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MS4</accession>
    <name>Q67MS4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2034</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Ycf16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2034"/>
    <dbReference type="EnsemblBacteria" id="BAD41019">
        <property type="protein sequence ID" value="BAD41019"/>
        <property type="gene ID" value="STH2034"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2034"/>
    <dbReference type="eggNOG" id="COG0396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_48_1_9"/>
    <dbReference type="OMA" id="MAMLEPK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="7"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="261" mass="28607" checksum="A1D4FF04C35FB000" modified="2004-10-11" version="1">MTTGVPLTVRNLHVSIDDKEILKGVDLEVKPGEIHAIMGPNGAGKTTLGFALMGHPRYEVTEGTVMLGDQNVLEMEVSDRAKAGLFLAFQYPFEVSGVTVANFLHQAVNAVRGEEISVWDFQEKLAEKMELLEMDESFAGRYLNEGFSGGEKKRNEMLQMLLLEPKIAILDETDSGLDIDALKVVAKAVNSLRGPNFGAIVITHYHRILDHIKPDVVHVLMDGRIVKTGGPELALEIEKKGYDWIKAELGLADEAAEAGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX6</accession>
    <name>Q67MX6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore peptidoglycan hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1982</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1982"/>
    <dbReference type="EnsemblBacteria" id="BAD40967">
        <property type="protein sequence ID" value="BAD40967"/>
        <property type="gene ID" value="STH1982"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1982"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037415_4_1_9"/>
    <dbReference type="OMA" id="IYARDWV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02874">
        <property type="entry name" value="GH18_CFLE_spore_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041704">
        <property type="entry name" value="CFLE_GH18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="45"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="2">
        <location>
            <begin position="77"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="419" mass="46969" checksum="10BE1963BC7EA4F3" modified="2004-10-11" version="1">MRPGESLWQIARRYGVTVQQIATLNGLRDANRLVPGMALLIPTERRHVVRAGETLWQIAARYGTTVDAIARENRIANVNLIYPGQTLVIPRGTRPVIEANAYQANLATGNVAPTAEAAPYLTYIAMFSAAVTEDGDVVPPRAESFIEAAHRGGAEALLTLTNFRGSTFSSDLASQFLNNPAAVQRAIDELVIILRVQGYAGLNVDFEYVYPSDREAYNNFLRQLRPRLHAEGRSLSTAIAPKVRADQPGLLYEAHDYPVHGEVCDFVVIMTYEWGYVTGPPMAIAPLRQVRQVLDYAVSAIPPEKILMGIPTYGRDWRLPYQRGVTRAETFSPVAATNRAIDVGAWIQYDEDAQSPFYRYWDAQGNEHIVWFEDARSLQAKFDTVKEYGLRGVSYWVLGNPFPQNLPLLQDNFRVRRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY2</accession>
    <name>Q67MY2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HflC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1976</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">HflC and HflK could regulate a protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the band 7/mec-2 family. HflC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196103.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1976"/>
    <dbReference type="MEROPS" id="I87.001"/>
    <dbReference type="EnsemblBacteria" id="BAD40961">
        <property type="protein sequence ID" value="BAD40961"/>
        <property type="gene ID" value="STH1976"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1976"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059167_1_0_9"/>
    <dbReference type="OMA" id="DFFAFYR"/>
    <dbReference type="OrthoDB" id="1714999at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052547">
        <property type="term" value="P:regulation of peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03405">
        <property type="entry name" value="SPFH_HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010200">
        <property type="entry name" value="HflC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42911">
        <property type="entry name" value="PTHR42911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005651">
        <property type="entry name" value="HflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01932">
        <property type="entry name" value="hflC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="4">
        <location>
            <begin position="38"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="304" mass="34011" checksum="B7AA2F74593D7353" modified="2004-10-11" version="1">MTPEREPIDLKIQVNPGEVKRLLAWIVAIAVIAGALSQVIFVREDEYLVIRSWTGVVQRVVTEAGPTFKIPLLQSAQTLPKHRVVHDSNPAELLTADQKPIIVDHYTVWQITDPRLFVQNTQTVARAEQRIDAAVYSTVRGVLGRLKFGEIISEGESARGNLNQEVTRLVNEQLASYGITVHDVRLKRTDLPPQNLESVFNRMKSERSKIAQDYLSQGDEQAAIIRARTDKEATLIVSEAARKAAEIEAEGEAEAARIFNEAYGADPEFYAFYRTLESYKTTLNGKPTIVIPIDSPYARLLMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MZ8</accession>
    <name>Q67MZ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1960</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196087.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1960"/>
    <dbReference type="EnsemblBacteria" id="BAD40945">
        <property type="protein sequence ID" value="BAD40945"/>
        <property type="gene ID" value="STH1960"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1960"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="CAILITG"/>
    <dbReference type="OrthoDB" id="1862837at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="32556" checksum="A7EBAF754500D7E3" modified="2004-10-11" version="1">MRSLDLVTRDLSKRFGERWALRGLSFAAVPGSVTLLAGRNGAGKTTWMRVATGLAHPSSGAVLFGGWPAGRVRTRLAAVFDDAPVYPLLTGAENLEVLSGGCPLRTAPARALVDELDLGSLLPLRADGLSFGQRKRLAVAAALLRRPSWLLLDEPSVGLDSGAWGAVAGTLRRLAAEGACIVVTGQDLKNLEELADQIVVIRDGAAVFAGTRAELQRLHPPRVRVRTPAADRLRALFPQAEVLAERPVPCLEIPCASGAEGEALLARIRASEIPLQSLELRAATLEESFRRLGLYDGEEQQEVAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N36</accession>
    <name>GCST_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminomethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gcvT</name>
        <name type="ordered locus">STH1922</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N36"/>
    <dbReference type="SMR" id="Q67N36"/>
    <dbReference type="STRING" id="292459.STH1922"/>
    <dbReference type="EnsemblBacteria" id="BAD40907">
        <property type="protein sequence ID" value="BAD40907"/>
        <property type="gene ID" value="STH1922"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1922"/>
    <dbReference type="eggNOG" id="COG0404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_10_2_9"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00259">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GCS_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022903">
        <property type="entry name" value="GCS_T_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GCST/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GCV_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCV_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="PTHR43757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="SSF101790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000122605" description="Aminomethyltransferase">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00259"/>
        </source>
    </evidence>
    <sequence length="375" mass="41243" checksum="EC3FF63B4CDC73DD" modified="2004-10-11" version="1">MNESLKRTPLYELHLKLGARMVPFGGWEMPVQYSSVIEEHRAVREAAGLFDVSHMGEFEVRGPQALDLIQLVSTNDAAKLAVGRVQYALMCYENGTVVDDILIYRLDEHRYWLVVNAGNTQKDWEWINTARERAGLHNLELIDRSAEIALLALQGPKAEEILQPLATGVVLSQLEPFSLAKNVTVSGVPTLVLSRTGYTGEDGFEIYVKAEDVAALWEALLEAGDEQGLLPCGLGARDTLRFEAKLPLYGHEISDQHNPLEAGLGFAVKLKKGVDFIGRDALARIKEQGPTRKLVGIEMIDRGVPRQGYPVAVGGEVVGEVTTGSFSPTLEKNIALAYVPVAHSAVGTEVEVIIRGRALKARVVETPFYRSPHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N62</accession>
    <name>Q67N62_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1896"/>
    <dbReference type="EnsemblBacteria" id="BAD40881">
        <property type="protein sequence ID" value="BAD40881"/>
        <property type="gene ID" value="STH1896"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1896"/>
    <dbReference type="HOGENOM" id="CLU_1757901_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="148" mass="15023" checksum="DD5E2595D023F3F5" modified="2004-10-11" version="1">MQRGTAFGLPEGGMAVRRLLYTIAALLLAVGSLGLAVLPRIAPGWLGFGVLGACFSGIGAAGWVLGGVAAGNWPRLRAGWFLLPAGLLGVVLSAMDHAGLPLFWAAGEAVTGLLLLVPLFPRHDGGESSGRPPGPVQAPPVAGRQEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N67</accession>
    <name>Q67N67_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">23S rRNA pseudouridine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196018.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1891"/>
    <dbReference type="EnsemblBacteria" id="BAD40876">
        <property type="protein sequence ID" value="BAD40876"/>
        <property type="gene ID" value="STH1891"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1891"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_8_2_9"/>
    <dbReference type="OMA" id="MIITKSH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="2">
        <location>
            <begin position="12"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="327" mass="35967" checksum="17D9537FC77E21D2" modified="2004-10-11" version="1">MRFVVDPSARGLRLSQYLFDRLKLSRTVVRRAKTSGGILVDGRPARTSLVLAGGEVVEVRVAQEGRVEPEPIPIQVVYEDPWLLVVDKPAGMVVHPVRDYVSGTLANAVAHHLLRSGSDPVARPVQRIDRETSGLVLFAKDPAVAGLLARELERQKLERRYVAVVEGAVAADAGTVDRPIRRVWGHPVAREVAEGPRTPEQERLLAEAAAQGRALREDWTAAGQPAVTHWRVLRRWPAATMLELRLETGRTHQIRVHMAYLGHPLLGDELYGGSRTLIQRQALHAATLAFVHPVTREPVRFTAPLPADLEDLVARLDRTAPNGSGAC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N86</accession>
    <name>BIOF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName>8-amino-7-oxononanoate synthase</fullName>
            <shortName>AONS</shortName>
            <ecNumber>2.3.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>7-keto-8-amino-pelargonic acid synthase</fullName>
            <shortName>7-KAP synthase</shortName>
            <shortName>KAPA synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>8-amino-7-ketopelargonate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-oxoamine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">STH1872</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (8S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195999.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N86"/>
    <dbReference type="SMR" id="Q67N86"/>
    <dbReference type="STRING" id="292459.STH1872"/>
    <dbReference type="EnsemblBacteria" id="BAD40857">
        <property type="protein sequence ID" value="BAD40857"/>
        <property type="gene ID" value="STH1872"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1872"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_0_9"/>
    <dbReference type="OMA" id="MDTHGFG"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008890">
        <property type="term" value="F:glycine C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010962">
        <property type="entry name" value="AONS_Archaea/Firmicutes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01825">
        <property type="entry name" value="gly_Cac_T_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381117" description="8-amino-7-oxononanoate synthase">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="206"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="237"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="392" mass="42720" checksum="EF839A3EFEA9E42E" modified="2004-10-11" version="1">MALTDFLVEELNGLKQAGLYRPLKELQSPQRPRSIIDGREVINLSSNNYLGLADDPRLKQAMIEATEAYGAGSGAVRTIIGTMTIHNQLEQKLAEFKHVEAAVVFQSGFTCNSGVIPVLVGEGDAVISDELNHASIIDGCRLSKAKIHRYKHADMDDLARVLKETDGQYRRRLIITDGVFSMDGDIAPLPDIVELAEKHGCMTYVDDAHSSGVLGKNGRGSVNHFGLDGRVTVQVGTLSKAVGVLGGYVAGPRALIELLWHKGRPFLFSTSHPPGVAAACLKAIEIMEQEPERIDRLWENTRYFKERLTELGFDTGKSETPITPVIVGDEVKAMQLSDRLLEEGVFAQGIAFPTVPRGKARVRTIVTAAHTKEDLDEALAAFAKVGRELGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB2</accession>
    <name>Q67NB2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exodeoxyribonuclease 7 large subunit</fullName>
            <ecNumber evidence="5">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exodeoxyribonuclease VII large subunit</fullName>
            <shortName evidence="5">Exonuclease VII large subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xseA</name>
        <name evidence="9" type="ordered locus">STH1846</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the XseA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1846"/>
    <dbReference type="EnsemblBacteria" id="BAD40831">
        <property type="protein sequence ID" value="BAD40831"/>
        <property type="gene ID" value="STH1846"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1846"/>
    <dbReference type="eggNOG" id="COG1570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023625_2_0_9"/>
    <dbReference type="OMA" id="WPAVRFE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04489">
        <property type="entry name" value="ExoVII_LU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00378">
        <property type="entry name" value="Exonuc_7_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025824">
        <property type="entry name" value="OB-fold_nuc-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13742">
        <property type="entry name" value="tRNA_anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00237">
        <property type="entry name" value="xseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_anti_2" evidence="8">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="7">
        <location>
            <begin position="132"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="7">
        <location>
            <begin position="338"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="415" mass="45942" checksum="848B17988500C2C7" modified="2004-10-11" version="1">MESFALAESGRQLLTVRELTRYVKDLLDEDPLLRSVWVQGEISNFKWHHSGHIYFTLKDEVAQVKVVMFRSYANRLRFRPENGMQVIVQGNVTVFERDGVYQLYAAAMEPYGLGALHLEFEQLKRRLAAEGLFDEALKRPIPSLPRAVGLVTAPTGAAIRDMITVARRRFPGMRLVLAPALVQGPDAPESLIRALGLVARVPEVDVVIIGRGGGSLEDLWAFNDERLARAIRACPVPVVSAVGHETDYTIADFAADLRAPTPSAAAELVVPSRSELLGVVDGLRIRLSTAARRSVERRRIRLRALTERPVLQRPQGRVLQDRQRLDGLVRRLEYLGGSLLAARRRELGGLAGRLEALSPLAVLARGYAIARTEEGRVVKDAAQLQVGDRLDVLLHRGSVRCQVEEIRGDAGEGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB8</accession>
    <name>Q67NB8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195968.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1840"/>
    <dbReference type="EnsemblBacteria" id="BAD40825">
        <property type="protein sequence ID" value="BAD40825"/>
        <property type="gene ID" value="STH1840"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1840"/>
    <dbReference type="HOGENOM" id="CLU_2482153_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="87" mass="9543" checksum="7CC5FBB1A91ACDA0" modified="2004-10-11" version="1">MEAPEVKIEVECAHCGNRKLVQLPGQWTPMPLNENNQPFPGPAMPVVLLGCTRCGYVQMFSPQAIKPKPFPERPAQQQGAQQDSGAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC2</accession>
    <name>ARGR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argR</name>
        <name type="ordered locus">STH1836</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Regulates arginine biosynthesis genes.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis [regulation].</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArgR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NC2"/>
    <dbReference type="SMR" id="Q67NC2"/>
    <dbReference type="STRING" id="292459.STH1836"/>
    <dbReference type="EnsemblBacteria" id="BAD40821">
        <property type="protein sequence ID" value="BAD40821"/>
        <property type="gene ID" value="STH1836"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1836"/>
    <dbReference type="eggNOG" id="COG1438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097103_3_0_9"/>
    <dbReference type="OMA" id="IMGTICG"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00173">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001669">
        <property type="entry name" value="Arg_repress"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020899">
        <property type="entry name" value="Arg_repress_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036251">
        <property type="entry name" value="Arg_repress_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020900">
        <property type="entry name" value="Arg_repress_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34471">
        <property type="entry name" value="PTHR34471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01316">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02863">
        <property type="entry name" value="Arg_repressor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01467">
        <property type="entry name" value="ARGREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55252">
        <property type="entry name" value="SSF55252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01529">
        <property type="entry name" value="argR_whole"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000205135" description="Arginine repressor">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00173"/>
        </source>
    </evidence>
    <sequence length="150" mass="16217" checksum="B4DC57B4722F04EB" modified="2004-10-11" version="1">MVKARRQAKILELVRTRVIETQEELAAALAAEGIPVTQATISRDIKELQLTKVPMPDGRYRYALPEEPGAAAGERWRRIFREAVLTIDHSGNLVVIKSLPGTAQGVAAAIDHAGWPEMIGTVAGDDTVIVVVKPADATGEVAERLKGLMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM2</accession>
    <name>LEXA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LexA repressor</fullName>
            <ecNumber evidence="1">3.4.21.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lexA</name>
        <name type="ordered locus">STH1736</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Ala-|-Gly bond in repressor LexA.</text>
            <dbReference type="EC" id="3.4.21.88"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S24 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.88" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195864.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NM2"/>
    <dbReference type="SMR" id="Q67NM2"/>
    <dbReference type="STRING" id="292459.STH1736"/>
    <dbReference type="MEROPS" id="S24.001"/>
    <dbReference type="EnsemblBacteria" id="BAD40721">
        <property type="protein sequence ID" value="BAD40721"/>
        <property type="gene ID" value="STH1736"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1736"/>
    <dbReference type="eggNOG" id="COG1974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_45_1_9"/>
    <dbReference type="OMA" id="HVWLLPH"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00015">
        <property type="entry name" value="LexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006200">
        <property type="entry name" value="LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006199">
        <property type="entry name" value="LexA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01726">
        <property type="entry name" value="LexA_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00498">
        <property type="entry name" value="lexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000170097" description="LexA repressor">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="1">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="91"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00015"/>
        </source>
    </evidence>
    <sequence length="205" mass="23097" checksum="8CAD209C0B362258" modified="2004-10-11" version="1">MPEPLTERQRQILQFIKDEIRTKGYPPSVREIGEAIGLSSSSTVHGHMTRLEEKGYIRRDPTKPRAIEVLDGSHTQLKRTIAVPVVGRVTAGQPILAQESIEDHFPLPADFVRADESELFFLTVQGDSMIEAGILDGDYVLVHRQQHANNGDIVVALIEDEATVKRFFKEQDHIRLQPENRFMDPIIVPDCQILGKVVGLVRRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR5</accession>
    <name>Q67NR5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1693"/>
    <dbReference type="EnsemblBacteria" id="BAD40678">
        <property type="protein sequence ID" value="BAD40678"/>
        <property type="gene ID" value="STH1693"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1693"/>
    <dbReference type="HOGENOM" id="CLU_1895131_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="134" mass="14737" checksum="EB880828D1C484C9" modified="2004-10-11" version="1">MGHAGTSRASSGGGSLTTIWRRGRTDPALRTDRNLCDNIMESGGGDMKEQITRTEVYSPSGRLYYIDVSDAGYVCVYDADGRRMLRVNLKGISAKAMVKRIRENIETGAPAYLAGWPPKDVDRILTQHAIACER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY6</accession>
    <name>Q67NY6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase_H_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1622</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1622"/>
    <dbReference type="EnsemblBacteria" id="BAD40607">
        <property type="protein sequence ID" value="BAD40607"/>
        <property type="gene ID" value="STH1622"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1622"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035904_2_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038720">
        <property type="entry name" value="YprB_RNase_H-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13482">
        <property type="entry name" value="RNase_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269578" description="RNase_H_2 domain-containing protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="RNase_H_2" evidence="3">
        <location>
            <begin position="113"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="437" mass="47833" checksum="D4EAC883C9E970C0" modified="2004-10-11" version="1">MRERLRLLKAAGAARAAAERAASPAAPASQASSGDPTRAPAGAPSALQPASLCPGLEGHLVATEAGPAFRLERRYPLDFRRGPLPLGWPLQLPATVWPLVGRVPPAFDPRRAAFVDTETTGLAGGTGTYAFLVGVGFYQGDEFVVRQYFLRDFPEEEAMLAAIAADLAPCTCLVSFNGKSFDWPLLETRFRLARRRPPLGGAPHLDLLHPARRLWSARLDGCTLQDLERAILGVVRTGDVPGSRIPALYFDYLRSGDASALVQVVEHNRLDILSLASLAGWMGQMAADPLGPTPDGELLCGDDLYHLGRLFEARGQLAEALTCYEAARQRGIAAVSETALLRRLSFAYKRARAHPQAVAIWEQMIASGGLTLFPYIELAKYHEHVTRRYDLAESLTVRALEVVDRRRSLGGAGAQREYQEVQHRLRRLRRKRTGQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P03</accession>
    <name>Q67P03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1605"/>
    <dbReference type="EnsemblBacteria" id="BAD40590">
        <property type="protein sequence ID" value="BAD40590"/>
        <property type="gene ID" value="STH1605"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1605"/>
    <dbReference type="eggNOG" id="COG1618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063820_0_0_9"/>
    <dbReference type="OMA" id="YYIPAMD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="35"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="387" mass="42229" checksum="AAB6032A3AFDF5B3" modified="2004-10-11" version="1">MCTMTRPGAVSHYFAGANTPGGFFSRYDQIAPADARKVFILKGGPGTGKSTFMKRIAEELLALGHDVEYFHCSADNEGIDAIYVPAADAAVLDGTAPHVVDPKYPGAVEEIINLGQFWDERALRSPEAKERIIRLTRGYKFWFQRANEARRAAQAWLDEWAAYHRACLDEDRMQAAGEAILAELFRGAPADGQDSTDPAPGRPGRARRLFASAITHGGPRSFLGPVFNGVRRRFVLVGPPGTGKKTIVQRVVDAALARGLDADVFHCTMYPERPDHVRLRALSAGVISSSWAHEYTPGPGDEVIDTGAFVDPAKLASYADAVAGAEAAYRAALRREVEHLGRAKAVHDELEQCYLPHMDFDGIERVRRETLERILACIAEGQADTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P07</accession>
    <name>Q67P07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ferrichrome ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1601"/>
    <dbReference type="EnsemblBacteria" id="BAD40586">
        <property type="protein sequence ID" value="BAD40586"/>
        <property type="gene ID" value="STH1601"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1601"/>
    <dbReference type="eggNOG" id="COG4606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_3_0_9"/>
    <dbReference type="OMA" id="WFAGSFT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="34949" checksum="80157DE1532812D9" modified="2004-10-11" version="1">MKRSLLIPLLTVLLVVLTVGSLFIGVTDISVTQVLRLTEDQRMVLMTSRVPRTAAIILAGVGMGVSGLIMQQLSRNKFVSPTTAGTDDSARLGILVAMILFGSATMLQKMVLALVFALAGTYIFMAILERVKLKDAVFIPLVGLMFGSIVSSITTFFAYRYDLLQAVTTWLQGNFALVIKGRYELLYFSVPAVIAAYIYANRFTIAGMGEDFATNLGVNYRSVVNVGLALVSLVSALVLLTVGMLPFLGLIIPNLVSIFQGDHLRRNLPLTALVGAVFLLACDIVGRLLIYPYEVPIGLTVGVIGSAMFLYLLWKGQRTREA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P26</accession>
    <name>SYN_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Asparagine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Asparaginyl-tRNA synthetase</fullName>
            <shortName evidence="1">AsnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">asnS</name>
        <name type="ordered locus">STH1582</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-asparaginyl-tRNA(Asn)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195711.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P26"/>
    <dbReference type="SMR" id="Q67P26"/>
    <dbReference type="STRING" id="292459.STH1582"/>
    <dbReference type="PRIDE" id="Q67P26"/>
    <dbReference type="EnsemblBacteria" id="BAD40567">
        <property type="protein sequence ID" value="BAD40567"/>
        <property type="gene ID" value="STH1582"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1582"/>
    <dbReference type="eggNOG" id="COG0017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_0_9"/>
    <dbReference type="OMA" id="DNMDLAE"/>
    <dbReference type="OrthoDB" id="1138123at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000176466" description="Asparagine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00534"/>
        </source>
    </evidence>
    <sequence length="437" mass="50445" checksum="22A2A82F8C1F30D0" modified="2004-10-11" version="1">MKWVTVDRLPQYEGQTVELRGWVQNYRSSGKIQFIIFRDGTGVCQAVLFIKDVPPEVFEAGKRLTQESSIIIRGSVRKDDRAPGGYEIGVQDLEIVQIAEEYPITKKEHGTEFLMDHRHLWIRSNRQVAILRIRNEITMAIHQFLQENGFVLTESPILMGTAAEGGATLFETTYVNDEPAYLSQSGQLHVEATAMALGRVYTFGPTFRAEKSKTRRHLIEFWMVEPEAAYFTHEDNMRLQEEMVTYVVRRVLERRSKELQLIGRDTTLLEKVEPPFPRITYTEAVEMLKKLHKPGDEWEPIEWGEDFGAPHETVLTQQFEKPVFVEKFPVKVKAFYMQPDPENPDVVLGADLLAPEGYGEIIGGSQRIHDPELLKRRFEEHGLDMNTYGWYYDLRRFGSVPHSGFGLGIERTVAWICGLEHVRETIPFPRLLNRLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P30</accession>
    <name>Q67P30_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1578</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40563">
        <property type="protein sequence ID" value="BAD40563"/>
        <property type="gene ID" value="STH1578"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1578"/>
    <dbReference type="HOGENOM" id="CLU_831159_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="35228" checksum="D974E5E125135E2B" modified="2004-10-11" version="1">MLLTDVALMEHRITLVGALGGLLAHAGRRLEPWYLAGVTGHAFRLTLDLVISPSAPHELNFHEVFPLWERLGVWFKRVAARPRDPGYADVRAEVIERVVQAVQRGRPAIVYDLLGCEEYGLVVGVEGDRWACLTPGAPTEPQWMEVASWPPADHAAFTRAEVIALLDLTPDFDRRAAEVASLRFAVDHFWAPASRDMWLQHGRQAYQFWRTTLAATGMPLHGPEPGRGHSYNLAVLHAARSDAAAYLADLAGRYPEADALQRAVGAYRRVADALAEASALAPYPGAPLAERRAEVAACLDRALAAETEGIEAIERALRALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P66</accession>
    <name>Q67P66_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cheR</name>
        <name evidence="7" type="ordered locus">STH1542</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1542"/>
    <dbReference type="EnsemblBacteria" id="BAD40527">
        <property type="protein sequence ID" value="BAD40527"/>
        <property type="gene ID" value="STH1542"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1542"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_1_1_9"/>
    <dbReference type="OMA" id="EWQEFVN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="29962" checksum="4892583D6ABC4B95" modified="2004-10-11" version="1">MNLPGYPVFQRTVLKLTGIDLDCYKGTQMERRLQTIMRRAGVQDLAEYARLLQTTPARVKEFQDFLTINVSEWLRNPDKFEELQQVILPELLKRNPVLRIWSAGCSNGAEPYSVAMLLDEIDPYGRHQIWATDLDAEILKVARAGVYQEKDIRNVSPERRAKYFTTVEEGFRVIDRLKARVRFEQHNLLSDPFPRDIDLILCRNVVIYFTEEAKDELYRKFHAALKPGGILFVGGTESLLKARELGFASASPFFYRAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC9</accession>
    <name>Q67PC9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1479</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195609.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1479"/>
    <dbReference type="EnsemblBacteria" id="BAD40464">
        <property type="protein sequence ID" value="BAD40464"/>
        <property type="gene ID" value="STH1479"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1479"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051308_0_0_9"/>
    <dbReference type="OMA" id="DDTRGLA"/>
    <dbReference type="OrthoDB" id="1015417at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="3">
        <location>
            <begin position="184"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="384" mass="41060" checksum="D14C727E7262FE9C" modified="2004-10-11" version="1">MILEHPTACRLVAEAELALPELVREVIAVCEIPAPTFHEAERAAYVERRMQEVGLREVRRDAVGNVIGRLRGNGTGPNLLIAAHLDTVFPAGTDVTVRPDGDILRAPGVGDNSSSVATMLHAARLLVKNDVPLAGDVIFAATCGEEGLGNLEGMRAVVEALKDEIDYVIALDGSLGGMVREGVGSRRFRLVVATSGGHSWGAFGAPSAIHSLGRIIARISELRVPTHPKTTYNVGVISGGTSINTIAARAEALIDLRSLDRGELVRLEERVLRIVRAEERESGVRATLELLGDRPTGSIPDTHPLCNLVRRVHRELGLQTRSYPSSTDGNIPLSMGIPAVTVGVTLGGNGHRLDEYIYTTPLTKGLAQVLLLVLGAQELPQRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF0</accession>
    <name>Q67PF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1458</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195588.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1458"/>
    <dbReference type="EnsemblBacteria" id="BAD40443">
        <property type="protein sequence ID" value="BAD40443"/>
        <property type="gene ID" value="STH1458"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1458"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_0_6_9"/>
    <dbReference type="OMA" id="HAGHRRY"/>
    <dbReference type="OrthoDB" id="1467120at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036244">
        <property type="entry name" value="TipA-like_antibiotic-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012925">
        <property type="entry name" value="TipAS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07739">
        <property type="entry name" value="TipAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89082">
        <property type="entry name" value="SSF89082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="29765" checksum="D9125FFA3367B050" modified="2004-10-11" version="1">MALTVKAVAELAGISVRTLHHYDEIGLLKPAGQSAAGYRLYSREDLERLQQILLFRELGFSLREIAAILESPGFDRRQALLAHREALLQRKARIERLIATVDRTLAAMEREDAPMDTREEMKELFDGFDPGEYEEEARQRWGGSREFRESAERTRKYTRQDWQRIAAEDGEIYGNIARLMDRDPADPAVQEWVGRWHEHISKWYYDCSLEVFRGLGEMYVQDERFTRNIDMTKPGLARFLRDAMQIYCDRREGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN9</accession>
    <name>Q67PN9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-glucose 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195499.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1369"/>
    <dbReference type="EnsemblBacteria" id="BAD40354">
        <property type="protein sequence ID" value="BAD40354"/>
        <property type="gene ID" value="STH1369"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1369"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_9"/>
    <dbReference type="OMA" id="HNTVSKR"/>
    <dbReference type="OrthoDB" id="1566711at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="304" mass="35765" checksum="7A4D9F80519CD130" modified="2004-10-11" version="1">MARILVTGSKGTLGSRLVQQLEQNGHEVWQMDTRHEADERYFRADVANWRQLERVFEQPYDYVYHLAAEFGRINGEEYYDTLWRTNVIGTRNILEFQRRKGFRLIFASSSEVYGETKAEVLDEDLPLRQPIIPHNDYALTKWVNEIQIMNFERRYEVPVVRLRFFNAYGPGERYHNYRSVVCLMAYRALTGRPWTVYEGYYRTFMYIDDFIPTLARVADRFKPGEVYNIGGTDFRSVRELSDLILQLTGADPGLVTYLPEDKHNTVSKRPDITKAMRDLGHNPKVTLEEGLPPTIDWLRREYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PP5</accession>
    <name>Q67PP5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195493.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1363"/>
    <dbReference type="EnsemblBacteria" id="BAD40348">
        <property type="protein sequence ID" value="BAD40348"/>
        <property type="gene ID" value="STH1363"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1363"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_372940_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="2">
        <location>
            <begin position="618"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="745" mass="81557" checksum="D9C3E5ABF506FC64" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ0</accession>
    <name>HSLO_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">33 kDa chaperonin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein 33 homolog</fullName>
            <shortName evidence="1">HSP33</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslO</name>
        <name type="ordered locus">STH1358</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Under oxidizing conditions two disulfide bonds are formed involving the reactive cysteines. Under reducing conditions zinc is bound to the reactive cysteines and the protein is inactive.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSP33 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195488.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PQ0"/>
    <dbReference type="SMR" id="Q67PQ0"/>
    <dbReference type="STRING" id="292459.STH1358"/>
    <dbReference type="EnsemblBacteria" id="BAD40343">
        <property type="protein sequence ID" value="BAD40343"/>
        <property type="gene ID" value="STH1358"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1358"/>
    <dbReference type="eggNOG" id="COG1281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054493_1_0_9"/>
    <dbReference type="OMA" id="DMQCECC"/>
    <dbReference type="OrthoDB" id="1406579at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00498">
        <property type="entry name" value="Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00117">
        <property type="entry name" value="HslO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000238101" description="33 kDa chaperonin">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="1">
        <location>
            <begin position="237"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="1">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00117"/>
        </source>
    </evidence>
    <sequence length="295" mass="31409" checksum="2D6ECF9AC6476B20" modified="2004-10-11" version="1">MADYLVRAIGAGGNVRAFAAVTTALTEEARRRHDTWPVATAALGRALTGTALLAATLKDPNESLTLRVAGDGPLRGILCDADEQGHVRGYVTEPHVDLEPAARGKLNVGAAVGTGMLYVTRQLALQGIYTGSSELVSGEIAEDLAYYLTRSEQTPSAVGLGVRVGPDGAVVAAGGYMVQLLPATPDADRDRLEENLGRLGSVSLAVEQGMTPEEILSVVLTGIDYQILERRDLSFPCRCSRERALGAIALLDETELSGLVEEGRGAELTCHFCNAVYRFSPEEVLQVQRGMKDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q04</accession>
    <name>Q67Q04_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1254"/>
    <dbReference type="EnsemblBacteria" id="BAD40239">
        <property type="protein sequence ID" value="BAD40239"/>
        <property type="gene ID" value="STH1254"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1254"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_9"/>
    <dbReference type="OMA" id="IIAGPYC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="391" mass="42189" checksum="35D4BA3B080DFC6D" modified="2004-10-11" version="1">MLDLSRVLAGPYCAQMLGDAGADVIKVESPAGDDTRAWGPPFLAGCEPQPGRPGDSAYYVSCNRNKRGIVLDLTTPHGQEALCRLVARADVLIENFKPGTMERWGLGYEDVLRPLNPRLVYASISGYGRTGPDADLPGYDFVAQAVGGIMSITGEPEGDPMKVGVAVTDLTTGMMAAFAICAALIARQATGRGQRVDLSLLETQVAWLANVGQSYLVSGQPPRRWGNAHASIVPYELFHAADRPIVVGVGNDAQFARFCAVLGRPEWAADERFATNPARLRHRRELVGLIARELRTRPAAEWLAAMRAAGVPSGPVRTIPEVFADPQVLARGMKVECNHPVAGLISLIGIPFKFSDTPATVRRPPPRWGEHTAEVLAEIGYSAEEIKQFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q31</accession>
    <name>Q67Q31_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1227</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1227"/>
    <dbReference type="EnsemblBacteria" id="BAD40212">
        <property type="protein sequence ID" value="BAD40212"/>
        <property type="gene ID" value="STH1227"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1227"/>
    <dbReference type="eggNOG" id="COG0325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_1_0_9"/>
    <dbReference type="OMA" id="IEWHMIG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="14"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="25687" checksum="2B02200495E84137" modified="2004-10-11" version="1">MSKIMENVSLVLERIRRAAERAARDPAGVRLVAVTKTRTVEEIEAVLAAGIRDLGENRVQELRSKAPFLQDRQPTWHLIGTLQTNKVKQALEWAELIHSLDRSALLSELVKQAERRGRPVDALVQVNVSGEASKHGLPPGDLEPFLRQVAQQRWVRVRGLMTMAPLSDNPEDARPHFRRLRQLRDQMQALGIEGVSLEHLSMGMSGDYEVAVEEGATLVRVGTAIFGPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q40</accession>
    <name>Q67Q40_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsA2</name>
        <name evidence="5" type="synonym">ftsA</name>
        <name evidence="8" type="ordered locus">STH1218</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Self-interacts. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="5">Localizes to the Z ring in an FtsZ-dependent manner. Targeted to the membrane through a conserved C-terminal amphipathic helix.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1218"/>
    <dbReference type="EnsemblBacteria" id="BAD40203">
        <property type="protein sequence ID" value="BAD40203"/>
        <property type="gene ID" value="STH1218"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1218"/>
    <dbReference type="eggNOG" id="COG0849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037850_3_2_9"/>
    <dbReference type="OMA" id="DGIIRHT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02033">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020823">
        <property type="entry name" value="Cell_div_FtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02491">
        <property type="entry name" value="SHS2_FTSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003101">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01174">
        <property type="entry name" value="ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="7">
        <location>
            <begin position="12"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR003101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="419" mass="42830" checksum="3F3A977AD7AACE55" modified="2004-10-11" version="1">MEGAGSVAENLVLGLDAGSTHVAAALAEAPEGGEPRVIGVGLVPSAGVYRGLISDLSAAARAMRKAAEAACAMAGRPQVNRAVISISGAHLRSEVGAAAVAVPRPAAGVTPELVRRVLDAAAEAVEPSAGRERVHVIPRSYQLDGSVPLRDPTGLCGRTLAAEVQVVTGDALHVQNHLRAASQAGFEVADYLVAVRAAGEAVLTPEEREEGVLLLDIGGGTTGVAVYELGHLFHLAVLPVGGDHITHDLATVLRIPVETAERLKRERGWAAPRLAGDGTVTLPTPSGLNTYEVSEKYVAEIIGSRVEEILQMAAAAVKRSGYAGLFPAGLVLTGGGSRLRGLAGYAGDCLSLKSRIGTPADSLAAEPELAAAAGLALWGVRAAPAAAEGGEPSEPATDAAEARPARAGRLRDWLKALFH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q93</accession>
    <name>QUEA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine:tRNA ribosyltransferase-isomerase</fullName>
            <ecNumber evidence="1">2.4.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queA</name>
        <name type="ordered locus">STH1165</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:32155"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="2.4.99.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueA family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.17" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195296.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q93"/>
    <dbReference type="SMR" id="Q67Q93"/>
    <dbReference type="STRING" id="292459.STH1165"/>
    <dbReference type="EnsemblBacteria" id="BAD40150">
        <property type="protein sequence ID" value="BAD40150"/>
        <property type="gene ID" value="STH1165"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1165"/>
    <dbReference type="eggNOG" id="COG0809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039110_1_0_9"/>
    <dbReference type="OMA" id="YSYGDGM"/>
    <dbReference type="OrthoDB" id="368001at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051075">
        <property type="term" value="F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00113">
        <property type="entry name" value="QueA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003699">
        <property type="entry name" value="QueA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042118">
        <property type="entry name" value="QueA_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042119">
        <property type="entry name" value="QueA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036100">
        <property type="entry name" value="QueA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30307">
        <property type="entry name" value="PTHR30307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02547">
        <property type="entry name" value="Queuosine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111337">
        <property type="entry name" value="SSF111337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00113">
        <property type="entry name" value="queA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000231382" description="S-adenosylmethionine:tRNA ribosyltransferase-isomerase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00113"/>
        </source>
    </evidence>
    <sequence length="341" mass="38185" checksum="FEB1F594D29330DA" modified="2004-10-11" version="1">MRLSDFDYDLPKELIAQKPVEPRDASRLMVVHRASGAIEHRCFRDLPEYLRPGDGLVINETRVMPARLLGSREQTGGAMEVLLLKRLDRDRWETLVKPGKKARPGERIVFGDGLLVGTVVGPTDYGGRVIDFHYEGVFENLLERLGQMPLPPYIHEQLEEPERYQTVYAREWGSAAAPTAGLHFTAELLDRLAARGVEIHRITLHVGLGTFRPVEVEDPTQHKMHSEFYHVSPEAAAGINAVRKGGGRLVAVGTTSVRTLETVADEDGTVRPGEGWTDIFIYPGYRFKAVDALVTNFHLPKSTLLMLVSALAGHDLIMRAYREAVAQRYRFFSFGDAMLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA0</accession>
    <name>Q67QA0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1158"/>
    <dbReference type="EnsemblBacteria" id="BAD40143">
        <property type="protein sequence ID" value="BAD40143"/>
        <property type="gene ID" value="STH1158"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1158"/>
    <dbReference type="eggNOG" id="COG1316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016455_5_3_9"/>
    <dbReference type="OMA" id="TGVRMDH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LytR_cpsA_psr" evidence="2">
        <location>
            <begin position="68"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="321" mass="35942" checksum="61D323A161276639" modified="2004-10-11" version="1">MLFSGSRRTIPQLTRWRRGDPNMATRRRTLSIVLAVLLLLLGGFAATAWFGTGPVNVLLMGLDEGRTRTDVVVLAHLEPRKGMLTLLSLPRDTLVEIDCAPLSEWCVTPDKLAHAHAYGGERGPEVTVETVERLLGVDIDHWVRVDFEGFQKVVDLLGGVDLVIDQDMHYEDPYADPPLHIHFEASEEPQHLDGQRALEFVRYRGVTGDIGRTERTKQFLVALARKARETQSLTRLPSAIRTGLAYVDTDIDVRTAFSLAIAALRVDLDRTRMETLPGWDDPGHRRGWVWVPDEEAVAEVVDRLILHPEPPADEPEVGSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA2</accession>
    <name>Q67QA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1156</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195287.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1156"/>
    <dbReference type="EnsemblBacteria" id="BAD40141">
        <property type="protein sequence ID" value="BAD40141"/>
        <property type="gene ID" value="STH1156"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1156"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_9"/>
    <dbReference type="OMA" id="EFVGTMS"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="347" mass="38135" checksum="977DC59B6A8B20A2" modified="2004-10-11" version="1">MSKVTIEGVVKRFEGHTAVNGVSLTVESGELFTLLGPSGCGKTTLLRVLAGFYRQDEGNVRFGDQLVNDVQPHKRNTGMVFQNYAVFPHMTVFDNVAYGLRARRVGGEELKRRVMEALEMVRMADLRDRMPAQLSGGQQQRVALARALVIQPQLLLMDEPLSNLDAKLRIEMRTEIRRLQRQYGITTIYVTHDQEEALAISDRIAVMNQGRVEQVGTPREIYFHPQTVFVAGFIGTTNLLRGRVEGGQVRALGAVVREVAAPDGDCTVAVRPEKIALGGGEVTFTGFVEEALFVGSATTVFVRTPAGERIEVRLPNEIDPPRVGEQATFGFRRAAAAVFGAQGEVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA6</accession>
    <name>Q67QA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1152</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1152"/>
    <dbReference type="EnsemblBacteria" id="BAD40137">
        <property type="protein sequence ID" value="BAD40137"/>
        <property type="gene ID" value="STH1152"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1152"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_1_3_9"/>
    <dbReference type="OMA" id="INECKDY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="23359" checksum="86EF30F17B4FC2DC" modified="2004-10-11" version="1">MKPLRGGDVVESAGAAGGLGMVGTGDPLRAAETGGGETEARPAEAAVEAWIQQHGEAVLRLAYASLLNRAQAEDVFQEVFIRAYRHADRLRDPDRVRPWLLQVTMNACRDLRRSWWWRRTREGSGLEGLAEAGEGPAADPAAAAVRADVSQAVARAVRALPDGFRETVVLHYFEGLDAAEIARITGVRVGTVHSRLHRARQLLRRQLEAWGVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE2</accession>
    <name>Q67QE2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1116"/>
    <dbReference type="EnsemblBacteria" id="BAD40101">
        <property type="protein sequence ID" value="BAD40101"/>
        <property type="gene ID" value="STH1116"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1116"/>
    <dbReference type="eggNOG" id="COG1078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_1_9"/>
    <dbReference type="OMA" id="FSEVYWH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="45"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="415" mass="47117" checksum="A10C6B318700E4A2" modified="2004-10-11" version="1">MQVRDPVHGDIYLTDVEVRVLDTREMQRLRSIKQLGTAHLVFPGCVHTRFDHSLGALHMAHRLMESLRLNGFTIAPEDAQLVRLATLVHDVTHIPYGHTFEDERQIFPRHDAGPRLEHFLGIQSELGRVLAASGYLDGVKAILTGRPPAPWMGQIVSSTIDADLLDYLRRDSFFAGLRMNYDDRVLSYFIKEDGRLLINMVKHGLDRPDARTEIIHLLRMRYVLTERLYLHHAKIAAGAMISKAVELHTRHTGLREEALYGLGDQTFLDLLARLPGPGIIQQLVEGVIERRLYKRAYVLSGDTLGPERDAFIARYGGQSAAREELEEQIAKKARLKPGQVIVYCPKASYFKEVQVPVRSRHGVGPLLELDRSGEVAAMARQYEGLWRTYVFCPADKVDAVQRACEKVFGYESEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QG3</accession>
    <name>Q67QG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Potassium uptake system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1095</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195227.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1095"/>
    <dbReference type="EnsemblBacteria" id="BAD40080">
        <property type="protein sequence ID" value="BAD40080"/>
        <property type="gene ID" value="STH1095"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1095"/>
    <dbReference type="eggNOG" id="COG0168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026429_0_1_9"/>
    <dbReference type="OMA" id="QATQFFI"/>
    <dbReference type="OrthoDB" id="756220at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004772">
        <property type="entry name" value="TrkH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00933">
        <property type="entry name" value="2a38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="456" mass="48058" checksum="6F9BB84FE715CED1" modified="2004-10-11" version="1">MALQHRRRGPTFTPPQALAAGFALLILIGTVLLALPVAHEPGHRLSLTDALFMATSAVCVTGLAVVDVSTTFSAFGEVVLLLLVQAGGLGIMSLSALMFLLTGRRIGLHERLMMQEALGSLSIAGVVRLTRTIIAGTLAVEAIGAALLSLRFLAYYPPAQALYFGIFHSITAFNNAGFDLTSRSLRPFQHDPAVLLVMAGLILMGGMGFVVLQDVWHHRRWERFSLQTKLVLAVTGVLVGAATLLVLALEWGNPATLGGLPVPEKLLNAFFTAVTFRTAGFESIPTAGMASAALLLAMVLMFIGGSPGGTGGGIRTTTFAVIALAVRATVRGTEDIQAMGRRLPRELLDRAIAIVAMSMAVIVAVGGLLLVTEGHLVTAPGNPFGAADVLFESTSAFTTMGLSTGVTPHLSMAGRLLLTVTMYVGRIGPLTAAVALAQRRRERVNIDYPEERVMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QI3</accession>
    <name>Q67QI3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195207.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1075"/>
    <dbReference type="EnsemblBacteria" id="BAD40060">
        <property type="protein sequence ID" value="BAD40060"/>
        <property type="gene ID" value="STH1075"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1075"/>
    <dbReference type="eggNOG" id="COG1876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054193_1_3_9"/>
    <dbReference type="OMA" id="FEGPHEK"/>
    <dbReference type="OrthoDB" id="1593541at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009045">
        <property type="entry name" value="Hedgehog_sig/DD-Pept_Zn-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003709">
        <property type="entry name" value="Pept_M15B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02557">
        <property type="entry name" value="VanY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55166">
        <property type="entry name" value="SSF55166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269314" evidence="2">
        <location>
            <begin position="28"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="VanY" evidence="3">
        <location>
            <begin position="123"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="29467" checksum="24E521AE24135BB3" modified="2004-10-11" version="1">MSFPSKQVRLTLLALLAAALAAGCAPGPQLPPPVPVDPTAEAPAPVEPEPEPDSGPVPDPGPGAADPEPADAPGGREVQLVADPTAIDVLVNKQYALPADYVPPDLVEPNVRFIFTEWHERRLMRAEAAAALEEMFAAAEEDGIYLAGVSGYRSYEYQEALFASYVSTDGLELAERYSARPGHSEHQTGLAMDISGSTGECAADDCFAGTPEAEWLAAHAHEFGFIVRYPKGKEHITGYMYEPWHVRYLGRELARAVYESGLTYEEYLQAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK8</accession>
    <name>Q67QK8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195182.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1050"/>
    <dbReference type="EnsemblBacteria" id="BAD40035">
        <property type="protein sequence ID" value="BAD40035"/>
        <property type="gene ID" value="STH1050"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1050"/>
    <dbReference type="eggNOG" id="COG1801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046519_0_0_9"/>
    <dbReference type="OMA" id="YLRMHGR"/>
    <dbReference type="OrthoDB" id="1068600at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002763">
        <property type="entry name" value="DUF72"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036520">
        <property type="entry name" value="UPF0759_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30348">
        <property type="entry name" value="PTHR30348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01904">
        <property type="entry name" value="DUF72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117396">
        <property type="entry name" value="SSF117396"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="34442" checksum="5EA0101ED6399DE2" modified="2004-10-11" version="1">MARIRVGTCAWSDHEEYYPPGLPPRERLSYYARHFSLVEVDSTFYRLQPARWFESWAERTPPDFRFNVKAYGAMTRHHREPRPGEEDVVEVFRRFDASVQPLREAGKLGALHFQFPPWFTCTEEARAWVRFCREFFADDLVAVEFRHVSWFRGESRDATLEFLRELRAVHVACDAPQVGTGTVPQVVAVTDPRLAIVRFHGRNAQTWYVKATTTAQRFDYLYSQEELAEWLPAIRDVLAPGAAEVHLLMNNNRANYAVRNALDLMELLGLPTPPRDERGVPLPPVRPRRTEQLRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN9</accession>
    <name>Q67QN9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195151.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40004">
        <property type="protein sequence ID" value="BAD40004"/>
        <property type="gene ID" value="STH1019"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1019"/>
    <dbReference type="HOGENOM" id="CLU_1569884_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="170" mass="19112" checksum="22722D63C599A2BB" modified="2004-10-11" version="1">MINALTREQVAGLRPPELQSYEEPLYEVGVPTLDLENRVPHRRLTSIEAAQLQAAFWQAEQHFGKEYDEVYRTLDELRERTEESGPLNEEWRRAIDAASNLHWNFARAIGVLAFLAGQREGFRLGAAAVTRGAQDQAIATALIGALVDDKQLDRLIGQLATGREDPEYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R11</accession>
    <name>Q67R11_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ion-translocating oxidoreductase complex subunit D</fullName>
            <ecNumber evidence="10">7.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Rnf electron transport complex subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rnfD</name>
        <name evidence="11" type="ordered locus">STH897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of six subunits: RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NqrB/RnfD family.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH897"/>
    <dbReference type="EnsemblBacteria" id="BAD39882">
        <property type="protein sequence ID" value="BAD39882"/>
        <property type="gene ID" value="STH897"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH897"/>
    <dbReference type="eggNOG" id="COG4658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042020_1_0_9"/>
    <dbReference type="OMA" id="GWQWINL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00462">
        <property type="entry name" value="RsxD_RnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004338">
        <property type="entry name" value="NqrB/RnfD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011303">
        <property type="entry name" value="RnfD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578">
        <property type="entry name" value="PTHR30578"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30578:SF0">
        <property type="entry name" value="PTHR30578:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03116">
        <property type="entry name" value="NQR2_RnfD_RnfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01946">
        <property type="entry name" value="rnfD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="modified residue" description="FMN phosphoryl threonine" evidence="10">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="315" mass="32587" checksum="FA07DA5E74FDA94B" modified="2004-10-11" version="1">MASSPHLWAGVSTARIMRDVLISLAPAALGAVCFFGWRAALIIALSMGAAALTEWGCMRLMGKPSTLGDGSALVTGLLLALTLPPASPVWLPVVGAAFAIAVVKVAFGGLGHNFVNPALAARAFLLAAFPAAMTTWTSPFDAVTTATPLAVLPSPFDPEVLAGPLPSYRDLFLGTVGGSLGETSALLLLIGAAYLLARRIIDWRIPAGFLGTLALLSWILSGQGGLFTGDPLYHVLAGGAILGAFFMATDYVTGPITPKGRWVFAVGCGLITVLIRLYGGYPEGVLFAILLMNVVTPLIDRFIVPAPFGGVKGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R23</accession>
    <name>Q67R23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative methyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH885"/>
    <dbReference type="EnsemblBacteria" id="BAD39870">
        <property type="protein sequence ID" value="BAD39870"/>
        <property type="gene ID" value="STH885"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH885"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_968779_0_0_9"/>
    <dbReference type="OMA" id="STSCFYL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="63"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="279" mass="32083" checksum="028CE249F553B423" modified="2004-10-11" version="1">MRLATRILLSLNKLFPPPVHPFNLANQGTMRYADWQFERGHLTLEHYRPFLSPEEIMRGKTVLDIGSGAGGKTLYYATLGVKKIYGVDVVPHYEHEARALAAEKNLSDRAEFLTADATALPFPDDHFDVIIANDVMEHVAQPEAVLREAYRVLKPGGRFFTNFPPYYHPYGAHLSDVIGIPWVHAFFSEPVLIEAYRELVKDLPDADMRLRLRLGNPETNRTRITYINRMTIKRFRRIVEESPFRIAHWRLVPLRSFLKPLAAVAPEFFMKAVVCILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R38</accession>
    <name>Q67R38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH870"/>
    <dbReference type="EnsemblBacteria" id="BAD39855">
        <property type="protein sequence ID" value="BAD39855"/>
        <property type="gene ID" value="STH870"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH870"/>
    <dbReference type="HOGENOM" id="CLU_097573_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="26535" checksum="891FF6FB5D976E94" modified="2004-10-11" version="1">MNVGQIFQRYFNDWQKFALVGLGALLVELAAGLIATVLGLLMMGPALVSIMRMEYLGLDIGPGQIFGMLGAMGGFAVIALVLGVVASGMANGGIMGAIVAYRRGEDVGLGTFWSYATRYFGKMILIGLIFFLIILVSAIVNLIPVLGQVVFLLWAPTALITLGLYPAYLVIHDGYGVGSAVGQGFQILKSQFGSAVLGGLIMLLFWVAFGLIGFVPVIGSLVVAIFGQPLVIYFFAERFESEVRPRLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R99</accession>
    <name>Q67R99_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH809"/>
    <dbReference type="ESTHER" id="symth-q67r99">
        <property type="family name" value="Carbon-carbon_bond_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39794">
        <property type="protein sequence ID" value="BAD39794"/>
        <property type="gene ID" value="STH809"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH809"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_9"/>
    <dbReference type="OMA" id="LLFWGKD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="46"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="281" mass="30425" checksum="2CCBDEBD9B2D2E2F" modified="2004-10-11" version="1">MRGSDAALSQSHQHFPLTGGTHMATVTLPSGLRLFYRERGHGGQTVLLIHGNTASSLWWERVMAHLPGHVRTLAPDLRGCGDSDKPAPPWSIADLAEDVYQFTQAMGVQRCFVVGHSLGGGVAMQLAVAHPDLVERLVLINSAPAEGLVTPPERYAQLEAAVKAPELLKAALALMMPTAPRDEFYQAVLEESVQKSAGALIPNGRALDGMNLVEQVGGLRVPTLILYGRQDGLVTLDMMERTRNQIPGAQLEVWPEVGHSAPVEAPERLAKRLIEFFGIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RA3</accession>
    <name>Q67RA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-(Acyl-carrier protein) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194938.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH805"/>
    <dbReference type="EnsemblBacteria" id="BAD39790">
        <property type="protein sequence ID" value="BAD39790"/>
        <property type="gene ID" value="STH805"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH805"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_9"/>
    <dbReference type="OMA" id="NAAHAHR"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="26368" checksum="A0EEDEB8AF1EE95C" modified="2004-10-11" version="1">MERELTGKVALVTGAARGLGRAIALEMAARGADVIVNDVPDMAEVAAETVAAAQALGVKARFYPAFVNDEEQVNRMAAEVGQVDILVNNAGVNRDGTMRKSSKADWDLVIDVNLTGTFNCLKAFTPAMRERRWGRVINIASYVGRTGVFGTPYYAASKAGVIGLTKEVAVEMARYGVTVNALAPGYIMTDMMMGYPEEQKAKITASIPVGYWAKPEDIAYWAGVLASPRAHYMTGAVIDCNGGVYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB5</accession>
    <name>Q67RB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DeoR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH793</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH793"/>
    <dbReference type="EnsemblBacteria" id="BAD39778">
        <property type="protein sequence ID" value="BAD39778"/>
        <property type="gene ID" value="STH793"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH793"/>
    <dbReference type="eggNOG" id="COG1349">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060699_1_4_9"/>
    <dbReference type="OMA" id="STHEPGF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014036">
        <property type="entry name" value="DeoR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00455">
        <property type="entry name" value="DeoRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08220">
        <property type="entry name" value="HTH_DeoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00037">
        <property type="entry name" value="HTHLACR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00420">
        <property type="entry name" value="HTH_DEOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="27919" checksum="BD79B5FFE70D47BF" modified="2004-10-11" version="1">MTGCDRMFPEERKRRIIERVRSGAAVTVQDLAQVFGVSESTIRRDLRELEREGLLERTHGGAVPADPTLTEPSYAEKTDQNRAEKMAIARVAAGMVHDGASIILDAGTTTLEIARLLKDRRNLTVVTNAYPIAAELADAPGVEVIVTGGTVRGKTLALVGPLAEQVLEQVNADLVFLGTNGIDIERGLTTPTPAEASVKRRMIAAARKVVVVADSSKAWRVAFAAVAPVSSMHMLISDRGLDPRLAHELLARGVQVLTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH8</accession>
    <name>Q67RH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome C-type protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH730"/>
    <dbReference type="EnsemblBacteria" id="BAD39715">
        <property type="protein sequence ID" value="BAD39715"/>
        <property type="gene ID" value="STH730"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH730"/>
    <dbReference type="eggNOG" id="COG3005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096753_0_1_9"/>
    <dbReference type="OMA" id="ACINCHV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3820.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005126">
        <property type="entry name" value="NapC/NirT_cyt_c_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038266">
        <property type="entry name" value="NapC/NirT_cytc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03264">
        <property type="entry name" value="Cytochrom_NNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="SSF48695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrom_NNT" evidence="3">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="153" mass="16479" checksum="8A8BE0FCE2DAEF50" modified="2004-10-11" version="1">MRNRWGKGALIALGALGGAFLILAFALQVPAIGHTIGAPAACGTCHVMTDQVLSLERGMHRELACVECHIPSGFFAKPIEEIKAASRHAAVFLTNSTPDVITATDSSRDIIQARCIECHAGFLRETSLVHHPTDGLKCFECHRDTTHGLPLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S13</accession>
    <name>Q67S13_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH545"/>
    <dbReference type="EnsemblBacteria" id="BAD39530">
        <property type="protein sequence ID" value="BAD39530"/>
        <property type="gene ID" value="STH545"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH545"/>
    <dbReference type="HOGENOM" id="CLU_896948_0_0_9"/>
    <dbReference type="OrthoDB" id="2110280at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="310" mass="35381" checksum="F4177F8639A72707" modified="2004-10-11" version="1">MMTVLHVVYDPHDPTAWYPNFLAGLRMGLDEAGVPYDYSPRMPERPRGPVFFTRYTVQKQLLEAAEPPEHVIIHEHDIWNPFTNAYDPADLWIYSHPSLAAILVTNPSMVPWGERYLPAGSRAQVVAAGFPYDHTLINRVIAELKPGPDRERLVVFPGRLNEFYQPYLSVRMAFELMDRGYRVFIASPVDPLSYYPVELWRQMGIEVGRLPHEDYYRLLARAQAAISVTTGGSLTLALYEAYLLGATPIAPAGRPDLPPWTEVYHPRYDALNPRQAIEMVEQRVPVAVECRWFDIGHYVEHLLAVAGPWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S61</accession>
    <name>Q67S61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194631.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH497"/>
    <dbReference type="EnsemblBacteria" id="BAD39482">
        <property type="protein sequence ID" value="BAD39482"/>
        <property type="gene ID" value="STH497"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH497"/>
    <dbReference type="HOGENOM" id="CLU_2107749_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="115" mass="11902" checksum="AF065B88203651A1" modified="2004-10-11" version="1">MNWDDMGTMPYGGTAMPMGMDGMPYGKWNMPYGKGGMPFGKGGMPFGKGGMPFGKGGMPFDKGMPFGKGGMPFGKGGFGKMPGWGGVPGGGFAKQYPPGMVGIPYGFYPGYGLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S85</accession>
    <name>Q67S85_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ComE-like competence protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH473</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH473"/>
    <dbReference type="EnsemblBacteria" id="BAD39458">
        <property type="protein sequence ID" value="BAD39458"/>
        <property type="gene ID" value="STH473"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH473"/>
    <dbReference type="eggNOG" id="COG0658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010363_2_1_9"/>
    <dbReference type="OMA" id="RTDKQGA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030420">
        <property type="term" value="P:establishment of competence for transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07731">
        <property type="entry name" value="ComA-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035681">
        <property type="entry name" value="ComA-like_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004797">
        <property type="entry name" value="Competence_ComEC/Rec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025405">
        <property type="entry name" value="DUF4131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13567">
        <property type="entry name" value="DUF4131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00361">
        <property type="entry name" value="ComEC_Rec2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="7">
        <location>
            <begin position="551"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="829" mass="86474" checksum="BA76BFE218DD4846" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S97</accession>
    <name>Q67S97_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative chromate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH461"/>
    <dbReference type="EnsemblBacteria" id="BAD39446">
        <property type="protein sequence ID" value="BAD39446"/>
        <property type="gene ID" value="STH461"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH461"/>
    <dbReference type="eggNOG" id="COG1300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069787_0_0_9"/>
    <dbReference type="OMA" id="QVWTNNA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="290"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="35468" checksum="0FE01FBAAB6934A3" modified="2004-10-11" version="1">MARRQEAWQELAGILAAAERRGPRRLSLEQVERLGLLYRRAASDLAYARTYFPNGQATAYLNQLVSRAHNLIYAEEPQRLRSLWRLFAHTVPATVRGAWRPLLLSAVLMGLGGLVGFFAILSDPNLAEALVPPELRHHEATAPDGDIFPVQFRSLIGTAILINNVRVGVMAFGLGITLGVGTALVLLYNGLIVGALAAHYFRAGLSFSFWALILPHGVLELMALFLAGAAGFCLGWPLVAPGRLTRGAAFRSGARRAGILILGSLPLFMVAAAIEGFITPMGSLSEWGKYGVALVTGALGVAYWALGGRRAAGAPADTDAQAPPAGTGSTAAPVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA6</accession>
    <name>Q67SA6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH452</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH452"/>
    <dbReference type="EnsemblBacteria" id="BAD39437">
        <property type="protein sequence ID" value="BAD39437"/>
        <property type="gene ID" value="STH452"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH452"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_9"/>
    <dbReference type="OMA" id="FFLDHNP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="5"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="333"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="576" mass="62232" checksum="AE15B633E7BC9CC0" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC5</accession>
    <name>Q67SC5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH433</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194567.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH433"/>
    <dbReference type="EnsemblBacteria" id="BAD39418">
        <property type="protein sequence ID" value="BAD39418"/>
        <property type="gene ID" value="STH433"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH433"/>
    <dbReference type="eggNOG" id="COG1534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095994_1_2_9"/>
    <dbReference type="OMA" id="FQVGKNG"/>
    <dbReference type="OrthoDB" id="2034603at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="98" mass="10737" checksum="C3F65B66C958290F" modified="2004-10-11" version="1">MELRGKQKRFLRAMGVTMSPILTIGKDGVTENVIRQADGALTARELIKGRVLQTAPAEPEAVAAQVAERTGAALVQVLGRNFLLYRRNPKEPKITLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF9</accession>
    <name>Q67SF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Septum site-determining protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194533.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH399"/>
    <dbReference type="EnsemblBacteria" id="BAD39384">
        <property type="protein sequence ID" value="BAD39384"/>
        <property type="gene ID" value="STH399"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH399"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_741713_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="373" mass="38134" checksum="4E8291F537A4F374" modified="2004-10-11" version="1">MTLRVALCGLPPAAARRLEGWLAAHPALQLSVSFSAPDVDSLLDRLSESPAHAVVLDAGLGLEGLSCAQELAAAGYAVLCLAGGPASAPLRRRAADLGLTLCPDADPARAATLLRRLLGLGAGSAQIGHVIAFHSPRGGAGTTSLLLHAARSLHGRGQTVAVVEVSGGGGAAPLLGLRPGGGWEELVGLPPEELLSDPCGPERVAAALRAVEPGLHLLPSAGPAVMDELHPDLVEAVLRLLGPCGCTFALVDTPAEMTLTAAAAIAASDAVCLIGLPDAVSAYRFVQVESLLAGLQVPPERVHPVLNRWREPAPPSVEEALAFLPYRPAVRVPEESRPAVDPSGRFCGFRPGGGAARALERLVDALVQEVAGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ6</accession>
    <name>ENGB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">STH362</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194496.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SJ6"/>
    <dbReference type="SMR" id="Q67SJ6"/>
    <dbReference type="STRING" id="292459.STH362"/>
    <dbReference type="EnsemblBacteria" id="BAD39347">
        <property type="protein sequence ID" value="BAD39347"/>
        <property type="gene ID" value="STH362"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH362"/>
    <dbReference type="eggNOG" id="COG0218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033732_3_0_9"/>
    <dbReference type="OMA" id="LMMDIRH"/>
    <dbReference type="OrthoDB" id="1746051at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000266971" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="EngB-type G" evidence="1">
        <location>
            <begin position="21"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="174"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="233" mass="26177" checksum="7540BA3EB54595AA" modified="2004-10-11" version="1">MGVHAEFVLSAVAQGQFPQDGLPEVALVGRSNVGKSSLINALVRNRKLARTSNTPGRTQALNFYRVWPQGKPRPEGEPQPDKDAGRTALSGPVLQAARESGAFYLVDMPGYGFARVSEAQRREWARLIEGYLLTRGALRGVLQIVDLRHPPTRDDVTMREWIRHHRLPSLCVATKADKIGRTAWPRHRQVIARELGLDGDGEPLVLFSAETGLGRDDVWRWIREHVQDWTFDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SL2</accession>
    <name>Q67SL2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH346"/>
    <dbReference type="EnsemblBacteria" id="BAD39331">
        <property type="protein sequence ID" value="BAD39331"/>
        <property type="gene ID" value="STH346"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH346"/>
    <dbReference type="eggNOG" id="COG2155">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2182643_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007211">
        <property type="entry name" value="DUF378"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37304">
        <property type="entry name" value="PTHR37304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04070">
        <property type="entry name" value="DUF378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="109" mass="11419" checksum="D395D1032833C857" modified="2004-10-11" version="1">MQQAPGGDLPKAFQILPQVLGPTGRPPCIGAAPPVHPTPQKGSGVRVFIARLLVIIGALNWGLIGLFGFDLVAFLFGGQMSLISRLVYTLVGVAGVFVIGDLVGNRQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP1</accession>
    <name>Q67SP1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH317"/>
    <dbReference type="EnsemblBacteria" id="BAD39302">
        <property type="protein sequence ID" value="BAD39302"/>
        <property type="gene ID" value="STH317"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH317"/>
    <dbReference type="HOGENOM" id="CLU_1947741_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="13747" checksum="4AAC415FBE926002" modified="2004-10-11" version="1">MAAGSSSTPPCFNPHPASRPGATRRSASPSRPRPRFNPHPASRPGATLPLDLLHRDGHHVSILTRPRGRVLRWAFFRPAAIRSFQSSPGLAAGCYPCIAAQIGCRVMFQSSPGLAAGCYPAQGLAHLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP4</accession>
    <name>Q67SP4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH314</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH314"/>
    <dbReference type="EnsemblBacteria" id="BAD39299">
        <property type="protein sequence ID" value="BAD39299"/>
        <property type="gene ID" value="STH314"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH314"/>
    <dbReference type="HOGENOM" id="CLU_2195597_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="108" mass="11339" checksum="362716B382A8AC78" modified="2004-10-11" version="1">MWPGATIGHLIDTAIVEGFQSSPGLWWGATSSSHRGAGRRRCFNSHSALWPGAAIMSKGHVCEGDAVSILTRPLGQVLPADDGDRLAVLVVSILTGLTAGRCLSTWPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ1</accession>
    <name>Q67SQ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39292">
        <property type="protein sequence ID" value="BAD39292"/>
        <property type="gene ID" value="STH307"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH307"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1980469_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="126" mass="14004" checksum="5A77C45EAECB1DBC" modified="2004-10-11" version="1">MVGVWPLPIHHVEVRLMQSHGTTPNIAAQAITSTTRHGFLVALGWVAQRLNLAEILNRHLRIKQKTYAHTPVDKVVEALVAILGNCRYMKDLNFDPEPLVADPAVAQAWGQERFAHFPPSVRPSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR0</accession>
    <name>Q67SR0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH298</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194433.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH298"/>
    <dbReference type="EnsemblBacteria" id="BAD39283">
        <property type="protein sequence ID" value="BAD39283"/>
        <property type="gene ID" value="STH298"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH298"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_8_0_9"/>
    <dbReference type="OMA" id="TDITMTI"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="88"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="295" mass="32284" checksum="9F38CA249AB44156" modified="2004-10-11" version="1">MNGRKKNGVNQDRFGGVRAFFRTIWRNPMSRAGFLILLVFLLMAIFGPMFIPELKSDYVNRLQPPSWKHPLGTDLAGKDTLLQLILGSRNVLLVAAYAGLFTVLIATGIGVISGLQGGVVDQVLMLLTNVVLTMPSFPVTMILSMIIRITDPLLFGVVLSLWSWAGLAKAIRSQVLSLKHREFIEASRLLGISKLNIIRYDVLPNLVSYVAVNFISIMKSAITSSVGLMLLGLVPFQGNHWGVMIQVAISQTGALMGSATVVYFLAPVVCLMLFQLGCYCFANGLDEALNPRLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SY7</accession>
    <name>Q67SY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH221</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194356.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH221"/>
    <dbReference type="EnsemblBacteria" id="BAD39206">
        <property type="protein sequence ID" value="BAD39206"/>
        <property type="gene ID" value="STH221"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH221"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_9"/>
    <dbReference type="OMA" id="CARMCPD"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="73" mass="7908" checksum="8CDFEF9A63F0E7C4" modified="2004-10-11" version="1">MGLRGIKLEMNQDRCKGCELCTEACPREVLAMGNQINALGYRAAVAIAPERCTGCRACALVCPEVVFTIYKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T26</accession>
    <name>Q67T26_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH182"/>
    <dbReference type="EnsemblBacteria" id="BAD39167">
        <property type="protein sequence ID" value="BAD39167"/>
        <property type="gene ID" value="STH182"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH182"/>
    <dbReference type="HOGENOM" id="CLU_1947740_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="14675" checksum="6295511D1B8975B7" modified="2004-10-11" version="1">MEGRDLLADLEDPELAQLVLNEPPPLPEDFTARVMARVEAERPRPFALLWPWLQGHWSVHQYASVAYALAATLVVVSLGNRFFLWSQATDRLAVWTAKGQAYWAAIQAWSGPLSERLLSMWYALTAFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T63</accession>
    <name>Q67T63_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH145</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194280.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH145"/>
    <dbReference type="EnsemblBacteria" id="BAD39130">
        <property type="protein sequence ID" value="BAD39130"/>
        <property type="gene ID" value="STH145"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH145"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_1_9"/>
    <dbReference type="OMA" id="MILASTY"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="98"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="327" mass="35986" checksum="5EA31BB61B8CA568" modified="2004-10-11" version="1">MSRYLLKRVLNMIPLLILATLLSFAIMQMAPGGPEKVLLAAEDATLDPTRIEELRERWGLNDPIPVQYVRWLGNVLRGDFGRSYFYRKPVVEVIGAALPATLKLQSVALFLTYAIAIPLGIISAVRQYSKLDYAVTGLAFAGQAAPSFWVAILLIWGVAMRSNGLIPTNGIATPGVNFETYGWLSVLADRAKYMILPVTVMVFGSLTGLTRYMRSAMLEVLKEDYIRTARAKGLSEKVVVYKHALRNALLPVISISGGLLSAMVGGSVITETIFSWPGIGRVGYDAVTQRDYNVSMAIMLMSGVLTLIGFLLVDIAYVWVDPRIKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T70</accession>
    <name>Q67T70_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.187</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">N-acetylmannosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">UDP-N-acetylmannosamine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">UDP-N-acetylmannosamine:N-acetylglucosaminyl pyrophosphorylundecaprenol N-acetylmannosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the conversion of GlcNAc-PP-undecaprenol into ManNAc-GlcNAc-PP-undecaprenol, the first committed lipid intermediate in the de novo synthesis of teichoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl diphosphate + UDP-N-acetyl-alpha-D-mannosamine = H(+) + N-acetyl-beta-D-mannosaminyl-(1-&gt;4)-N-acetyl-alpha-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:16053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:62959"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:132210"/>
            <dbReference type="EC" id="2.4.1.187"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; teichoic acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 26 family. TagA/TarA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.187" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH138"/>
    <dbReference type="EnsemblBacteria" id="BAD39123">
        <property type="protein sequence ID" value="BAD39123"/>
        <property type="gene ID" value="STH138"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH138"/>
    <dbReference type="eggNOG" id="COG1922">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063203_3_1_9"/>
    <dbReference type="OMA" id="PWRWRRM"/>
    <dbReference type="UniPathway" id="UPA00632"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047244">
        <property type="term" value="F:N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019350">
        <property type="term" value="P:teichoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06533">
        <property type="entry name" value="Glyco_transf_WecG_TagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02070">
        <property type="entry name" value="TagA_TarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034714">
        <property type="entry name" value="TagA_TarA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004629">
        <property type="entry name" value="WecG_TagA_CpsF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34136">
        <property type="entry name" value="PTHR34136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03808">
        <property type="entry name" value="Glyco_tran_WecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00696">
        <property type="entry name" value="wecG_tagA_cpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0777">Teichoic acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="250" mass="27205" checksum="E68CA6646761C938" modified="2004-10-11" version="1">MADDMKRAQILSIGVDKVTMAEAVDRCLGFIDAGGPHLVVTPNAEIADRAGRDPELAAIINGADLVVPDGAGIVLASRLLGDPVPEKVGGADLASNLLKALNDRGGGRVFLLGTRPEVVAEAARRVQEAYPRIQVVGWRDGFFRPEEEGEVVAQIRAARPDVLFVALGSPKQERWLHRHLPELGVPVGMGVGGTIDVWAGAVPRAPQWMIRANLEWLYRIVRLRRVRRSLPPLVRFGLNVLRARAQRPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA2</accession>
    <name>Q67TA2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194241.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH106"/>
    <dbReference type="EnsemblBacteria" id="BAD39091">
        <property type="protein sequence ID" value="BAD39091"/>
        <property type="gene ID" value="STH106"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH106"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_9"/>
    <dbReference type="OMA" id="QIILRQM"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="303" mass="33191" checksum="8E51E551685705EF" modified="2004-10-11" version="1">MTNELDIFCAVAKHGSMSRAAAELHLSQPAVSQRLRALEEQYGLQLFRRTNRGVELTPAGETLVRYAQRILQLERSLQAEMASLRAAEPKQVVIGATSAIGGYALPCTVYLFQQKYPDARIQLQIGKRCDVIQRLEDGLVDLALVEGPLVTALPDDWQVSVISEEELVLITPPTGPLAGKEAYTADDLTRLPLIIREPGSGTRMAVEEGWQAAGRDLRDLNIALELTGVDAIKTSVASGHGVALVSRWCVRAEARMGHLRIAPLTDIAFTSRWTLIVPKQGMKGAMERALLRTLRSPAERGFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE8</accession>
    <name>Q67TE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative carboxy-terminal processing proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH60</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH60"/>
    <dbReference type="EnsemblBacteria" id="BAD39045">
        <property type="protein sequence ID" value="BAD39045"/>
        <property type="gene ID" value="STH60"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH60"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017295_3_0_9"/>
    <dbReference type="OMA" id="IVTIEYN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269378" evidence="4">
        <location>
            <begin position="30"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="97"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="465" mass="50282" checksum="7227CC39FE946744" modified="2004-10-11" version="1">MVARLWRSFAASLLALFLTAALWTGPAYAASDPAHWGDASAELWMVYHVVQRYHVNGADLDAFLEGAVRGGLDALGDPYTEYLGPQDYQSFLDSVDGNLTGIGVYLEKVGDYITIVRPIRSSPAEAAGLRAGDRIVAADGVSLVGESIEKTQQLVRGEPGTKVVLTIERPDEGRRFDVTITRAFIHVPQVDYRMLEDGIGYLELSGFGQQAAEEFFAAVAELKEAGATRLVLDLRYNSGGWVASALEIAEAYVPAGEPIMFEITRDERRVYRSEGSPLGLPTAVLVSEYTASAAEILAGAVQDYGTGVLIGTQTFGKGTVQELVDLPGGGAVKVTTAEYLTGRQRRVHQVGLTPDLVVEPYQPPVHLQTPLVADRVMTTGHVGLDVLGLQERLALLGYRTDRDGYFKGQTTLAVLQFARDHGLNEVPMVDKAFVEALNAAVAAKLAAAEWPDTQLEAAIRYLKEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ3</accession>
    <name>RECR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Recombination protein RecR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recR</name>
        <name type="ordered locus">STH15</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecR family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194150.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TJ3"/>
    <dbReference type="SMR" id="Q67TJ3"/>
    <dbReference type="STRING" id="292459.STH15"/>
    <dbReference type="EnsemblBacteria" id="BAD39000">
        <property type="protein sequence ID" value="BAD39000"/>
        <property type="gene ID" value="STH15"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH15"/>
    <dbReference type="eggNOG" id="COG0353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060739_1_0_9"/>
    <dbReference type="OMA" id="DVMAIEN"/>
    <dbReference type="OrthoDB" id="1661836at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01025">
        <property type="entry name" value="TOPRIM_recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00017">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000093">
        <property type="entry name" value="DNA_Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023627">
        <property type="entry name" value="Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023628">
        <property type="entry name" value="Rcmb_RecR_C4-type_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015967">
        <property type="entry name" value="Rcmb_RecR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034137">
        <property type="entry name" value="TOPRIM_RecR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30446">
        <property type="entry name" value="PTHR30446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02132">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111304">
        <property type="entry name" value="SSF111304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00615">
        <property type="entry name" value="recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01300">
        <property type="entry name" value="RECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000190407" description="Recombination protein RecR">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="80"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00017"/>
        </source>
    </evidence>
    <sequence length="198" mass="21958" checksum="79C9B9EC915F7912" modified="2004-10-11" version="1">MYYAEPIARLIEELTKLPGIGPKTAQRLAFHILHMEPSVVEGIARTLVEARAKVKYCSVCCNLTDQDPCQICADDGRDHSTICVVQEPRDVVAMEKTREYHGVYHVLHGALNPMEGIGIDDIRVKELVARLGDGRVQEVILCTNPNTEGETTAMYIARYIKPLGVKVTRIARGLPMGGDLEYADEVTLAKALEGRREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG6</accession>
    <name>Q67JG6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin-guanine dinucleotide biosynthesis protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3202"/>
    <dbReference type="EnsemblBacteria" id="BAD42184">
        <property type="protein sequence ID" value="BAD42184"/>
        <property type="gene ID" value="STH3202"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3202"/>
    <dbReference type="eggNOG" id="COG1763">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068199_2_2_9"/>
    <dbReference type="OMA" id="HTHHNMD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MobB" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="18280" checksum="772C419D524DA708" modified="2004-10-11" version="1">MLSIVGCGKCGKTTLVERLVAELTRRGWSVGTLKHDVHGFQMDHEGKDTWRHRQAGAKAVCIIGPGQIGLVRSVPEGEFSTADAIALLGSVDLVITEGFKRERFPKIEIFSSARNEGHLLCGEDPTLIAVAGDLPVQTALPRFDWNDIVAIADFVEMRLLRDAQTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ2</accession>
    <name>Q67JJ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Succinate dehydrogenase cytochrome b558 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3176</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3176"/>
    <dbReference type="EnsemblBacteria" id="BAD42158">
        <property type="protein sequence ID" value="BAD42158"/>
        <property type="gene ID" value="STH3176"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3176"/>
    <dbReference type="eggNOG" id="COG2009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078991_0_0_9"/>
    <dbReference type="OMA" id="YDMMANI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03497">
        <property type="entry name" value="SQR_TypeB_1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011138">
        <property type="entry name" value="Cytochrome_b-558"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016002">
        <property type="entry name" value="Succ_DH_cyt_b558_Firmicute"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000170">
        <property type="entry name" value="Succ_dh_cyt_b558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02046">
        <property type="entry name" value="sdhC_b558_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="23743" checksum="6223BF2DA7050E39" modified="2004-10-11" version="1">MRRNHFLLRRLHSLAGVVPVGLFLVFHLFENSKAMYGPEAYNEVVLSINSLPFILFIEIFGIFLPLYFHAIYGIYIAADARHNVSQYSYGRNWAFLLQRITGLVTLVFVTYHIWHFRVQKALGVYDGNLELPGLPSYTIVAEAFASPLAKVLYGIGIVAAAYHFANGLYTFLITWGITIGPRSQRVSSLVTNLLFVLVSAMGLTALFAFQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK1</accession>
    <name>Q67JK1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Pyruvate flavodoxin dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH3167</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 3 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the pyruvate:ferredoxin/flavodoxin oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197280.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3167"/>
    <dbReference type="EnsemblBacteria" id="BAD42149">
        <property type="protein sequence ID" value="BAD42149"/>
        <property type="gene ID" value="STH3167"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3167"/>
    <dbReference type="eggNOG" id="COG0674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_0_0_9"/>
    <dbReference type="OMA" id="NTVMQVC"/>
    <dbReference type="OrthoDB" id="10483at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016903">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037112">
        <property type="entry name" value="Pyrv-flavodox_OxR_EKR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019456">
        <property type="entry name" value="Pyrv-flavodox_OxRtase_EKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011895">
        <property type="entry name" value="Pyrv_flavodox_OxRed"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10371">
        <property type="entry name" value="EKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000159">
        <property type="entry name" value="NifJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00890">
        <property type="entry name" value="EKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
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        <source>
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        </source>
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    <sequence length="1183" mass="128883" checksum="009B703237F72C23" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS7</accession>
    <name>Q67JS7_SYMTH</name>
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        <recommendedName>
            <fullName evidence="7 8">Transcription termination/antitermination protein NusG</fullName>
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    </protein>
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        <lineage>
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            <taxon>Clostridia</taxon>
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            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Participates in transcription elongation, termination and antitermination.</text>
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    <comment type="similarity">
        <text evidence="7 8">Belongs to the NusG family.</text>
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    <dbReference type="OrthoDB" id="2027986at2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
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    </dbReference>
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    <feature type="domain" description="NGN" evidence="9">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="20131" checksum="EBCE3E141A010C97" modified="2004-10-11" version="1">MAEKHWYVVHTYSGYENKVKTNLEKRVESMEMQEKIFRVLVPMEDEIEIKDGKQKKVKRKVFPGYVLVEMVMSDDSWYVVRNTPGVTGFVGSGNKPIPLTPEEVEAILGRHGEDKPAHVVVDFEVDQPVRVIAGPFKDHTGVIKEIFPEKGKARVQVTMFGRETPVEVDFSQIEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT6</accession>
    <name>Q67JT6_SYMTH</name>
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        <recommendedName>
            <fullName evidence="1">MTS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197197.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3082"/>
    <dbReference type="EnsemblBacteria" id="BAD42064">
        <property type="protein sequence ID" value="BAD42064"/>
        <property type="gene ID" value="STH3082"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3082"/>
    <dbReference type="eggNOG" id="COG2813">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_7_2_9"/>
    <dbReference type="OMA" id="HMIDVNE"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="28"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="206" mass="22386" checksum="557FDE38531A15E8" modified="2004-10-11" version="1">MSEHYYTRRPGVAHAETSFSVTLRGMDFRFVTDAGVFSRERVDFGSLLLIEAMEIGPADTVLDLGCGYGPIGIVAARLAPQGFIYMVEINERAAELARRNLEANAIANAEVRVGDGLEPVAGIAFDAVVTNPPIRAGKATVYRLLDEAHAALKPGGSLWVVVQNKQGAPSMKRKLEALFGNVEDVARQAGYRVLRARREDKPKMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JZ1</accession>
    <name>Q67JZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine proteinase inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3026"/>
    <dbReference type="EnsemblBacteria" id="BAD42009">
        <property type="protein sequence ID" value="BAD42009"/>
        <property type="gene ID" value="STH3026"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3026"/>
    <dbReference type="eggNOG" id="COG4826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023330_4_0_9"/>
    <dbReference type="OMA" id="DSINMWV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="PTHR11461"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="SSF56574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERPIN" evidence="2">
        <location>
            <begin position="32"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="388" mass="42489" checksum="DFAD443BFB5534E1" modified="2004-10-11" version="1">MTGCGSPGLASPPPRPDAVDPALSEAADRFGIELLRAVYDQRPDANLLLSPASAWVILSLAANGARGETQREMLAALGYGDAEMGAVNAHMRDVWGIMANPGRGVKLTAANAVWHHRDFAVVPAFREVAAKQYGAEVFGTTFGRPEAAAAINRWVARRTRDRITDLIDRTYAEQRMALVNALHFKGEWQEPFDPDRTEPGPFRRPDGSEVQVEFMHQTETFGYLAEEGLVGVRMPYGEGDVALYAFMPDEWDGFVVGLTPERFRGWIDALAETRIRLAMPKVRFTDRHDLSEALQALGMERAFDDRRADFGGLLVTDEPLYIGAVIQKTFLEINEEGTEAAAATVVGITAGAAPPPQPPEVVLDRPFLLAIRDDRTGMTLFLGAVVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K70</accession>
    <name>Q67K70_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2945"/>
    <dbReference type="EnsemblBacteria" id="BAD41928">
        <property type="protein sequence ID" value="BAD41928"/>
        <property type="gene ID" value="STH2945"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2945"/>
    <dbReference type="eggNOG" id="COG3278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034656_1_0_9"/>
    <dbReference type="OMA" id="AMTQFVP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="439" mass="47456" checksum="4CB6EB9D5A06DE9E" modified="2004-10-11" version="1">MKGPKKAGPQVIDPALSPPIYVPIGMALAGVLGLLVLQALVIAKAPMIFGPFRFNPAALAAVHLFTLGFATSVMIGAFHQITPVMMRGRPVEGGWALLQGLAYLAGSWALVWGFYRSAGPWITAGGTLVVLALVVFAALVVRAMRSATQWTVAGRFMAAALSFVLGTVIWGLVLAFNLRRGFLPDSLDYSPLGAHVLLGLGGWFMLTVFGVSYQLFPMFALTNRPSAASGHAVLAKLSAGLWGAFASLLLHLPALTAACLLLAAAGAARYAADFFAMMRQRRRPELDLSMRYGVTALAFLVPAALLLLLSVRHRAFAVPGAWLFLFGFVATMILGMLYRIIPFLFWHHLMRNRRSKTQRLPKLDEMFAQRTARAGYFCWTAGVLLTGLLLLGGALGWWDARYLVRGSAILNLTGSLLFAGTILQVLRARPLDGRERSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC1</accession>
    <name>Q67KC1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2894"/>
    <dbReference type="EnsemblBacteria" id="BAD41877">
        <property type="protein sequence ID" value="BAD41877"/>
        <property type="gene ID" value="STH2894"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2894"/>
    <dbReference type="HOGENOM" id="CLU_1427345_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="190" mass="20969" checksum="679D847F33C17DC5" modified="2004-10-11" version="1">MRDHSQVGRDSVDGPYLERPDTRAEQYLVGALLAAVCLAAGWLVVRIRSTPGAAHPLEFTGLFLLLALAAYLGHAGYELSTVRYALRDQRFRVAQGRRAVELDLRQPLRLHRWLNRWDGSGAAAAELGVAEVEWYPPVALVRTACWVVVGHDPAGLCRAVAVRPSPRLLALLREMAVPKWGEEVGETADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KI6</accession>
    <name>Q67KI6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Murein transglycosylase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196946.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2827"/>
    <dbReference type="EnsemblBacteria" id="BAD41812">
        <property type="protein sequence ID" value="BAD41812"/>
        <property type="gene ID" value="STH2827"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2827"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1299207_0_0_9"/>
    <dbReference type="OMA" id="AGWETNP"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269516" evidence="2">
        <location>
            <begin position="31"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="3">
        <location>
            <begin position="85"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="212" mass="22939" checksum="96E74B5C3F8BFF31" modified="2004-10-11" version="1">MRQRLRRRGFLWAALALGVAAVPTMLAATAAEPAQDAEPVLPEPEPDPTAELPPDPAELGRARLEELAARIEDLAPEEYRPLVVEVADRYGVDPRLIAAVITVESRWDPDAVGAHGERGLMQILPSTGQWLAGVMGLEAYDLSDPATSVEMGTFYLSALIAEYGSVDVALAVYNGGPRAAAGWETNPYRERVLAAYSSTFPREQRELLDLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KN2</accession>
    <name>Q67KN2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2781"/>
    <dbReference type="EnsemblBacteria" id="BAD41766">
        <property type="protein sequence ID" value="BAD41766"/>
        <property type="gene ID" value="STH2781"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2781"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1244806_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="222" mass="24520" checksum="11414418D097645B" modified="2004-10-11" version="1">MGERLAWARNQQGLILQQVSEKSGLAVGYISQLEKGAKVNPTIDALGRLAKALGVSIAFLLGEVHAPPYDDRAALLIGGQAWTVGQRFARYYEQLSPEERRKIELETVEQRFARVVDFLCQEFPETFTRPVVAYQLGLSVRALNDIMERNCEVGPMVLSQMVSITGIPLSFYARGRMESAPVDITPAQMIRYAEAISMAAEAQITPEELVLLIRGASQAHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR4</accession>
    <name>Q67KR4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Iron ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2748"/>
    <dbReference type="EnsemblBacteria" id="BAD41733">
        <property type="protein sequence ID" value="BAD41733"/>
        <property type="gene ID" value="STH2748"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2748"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_0_2_9"/>
    <dbReference type="OMA" id="NNPIADV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026045">
        <property type="entry name" value="Ferric-bd"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002825">
        <property type="entry name" value="CfbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="365" mass="41100" checksum="58E0B16BCCC6DDAE" modified="2004-10-11" version="1">MLVIGLFAAGCGGSGQSPNNNQGGTQTPSNSQTQTQQPQTQEPQEEKRLVIYTGRDQSIIDLMVAKFEEKYPEYKGNVEVLPMGAQEIMERVRAEKANPQGDIWWGGTQQALSLAVKEGLLAKINPEFASKIADGYKDPEGHWYGEMLLPEVIMYNKEMISESELPKDWDDLLDAKWKDQIIIRAVPASGTMRTIYAAMIDRFHQEDGKPDRGYEWLLKLDANTKEYAANPTDLYLKIFRQEGSLSLWNLQDVLIQAQEGMPFGYIIPESGVPILVDGVGMIAGAKHPQAAEKFLNLLFSDEVQAELAATRFQIPSINLPESMQPEWLKELMPQLKAMDLEWDVMAEKEAEWIGYWDENIKGKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR5</accession>
    <name>Q67KR5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NPCBM_assoc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2747"/>
    <dbReference type="EnsemblBacteria" id="BAD41732">
        <property type="protein sequence ID" value="BAD41732"/>
        <property type="gene ID" value="STH2747"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2747"/>
    <dbReference type="eggNOG" id="COG1463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_347750_0_0_9"/>
    <dbReference type="OMA" id="HKSQGFG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018905">
        <property type="entry name" value="A-galactase_NEW3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10633">
        <property type="entry name" value="NPCBM_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="NPCBM_assoc" evidence="2">
        <location>
            <begin position="439"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="880" mass="95968" checksum="AF8F3912D7875E5E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX4</accession>
    <name>Q67KX4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="3 7">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">leuA</name>
        <name evidence="9" type="ordered locus">STH2687</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="3 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2687"/>
    <dbReference type="EnsemblBacteria" id="BAD41672">
        <property type="protein sequence ID" value="BAD41672"/>
        <property type="gene ID" value="STH2687"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2687"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_9"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01025">
        <property type="entry name" value="LeuA_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005671">
        <property type="entry name" value="LeuA_bact_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00973">
        <property type="entry name" value="leuA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="523" mass="56994" checksum="BEF97922A83A39BC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY5</accession>
    <name>Q67KY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196798.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2676"/>
    <dbReference type="EnsemblBacteria" id="BAD41661">
        <property type="protein sequence ID" value="BAD41661"/>
        <property type="gene ID" value="STH2676"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2676"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_9"/>
    <dbReference type="OMA" id="KGIQQNM"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="298" mass="33890" checksum="1B874A07A8E47366" modified="2004-10-11" version="1">MLLSYLRVYCDIIETRSFSKAAARNFISQPAVSQQVKAMEEHFQQRLIERSPQGIYPTEAGRIFYEAAREIVERYQALEQQMANLSQSVSGTIRVATVYTVGLHDLPPYIKKFIQTYPQARIHLEYSRTNKIYEAVRQNQVDIGIVAYPQEGRQLGVIPLPSDELVVIVSPNHPLARAGKPVALQQLHRLPFIAFDPDIPTRKATDRVLNDHGVDVEIIHEFDNVETIKRSVEAELGVSIIPRRCAQWEVRSGSLVALPIADMRIERPVGVIYKRGKNFSNTLQKFIAILTGREEQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L57</accession>
    <name>Q67L57_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196726.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2604"/>
    <dbReference type="EnsemblBacteria" id="BAD41589">
        <property type="protein sequence ID" value="BAD41589"/>
        <property type="gene ID" value="STH2604"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2604"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_9"/>
    <dbReference type="OMA" id="HYLDDEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="291" mass="31296" checksum="423A4E648055F104" modified="2004-10-11" version="1">MLHLLESFCAAAETGSLNKAAEMLHLSQPAITRQIKSLEAQLGAVLLTRTPRGVELTPAGQAVLPHARAALAAVAACRHAAAQASGAGPGRLRIAAGMIITLYTLPPVITAFREAYPAIPVDLRPGHNREVLHQLLEYEADVAFLASRVSAPGVKAIKLFSDPMLLVTHPAAEPLERLRDLAGRTLLVLQPGAGMRHEVEEALTEHGVSCRLVEHPTVETIKTAVMLQMGVTILPASAVREEVRDGRLAARPVADWPGSGRTVHAYVRAEGVLPEQVRTFIHMVKQHYRDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-06-16" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L72</accession>
    <name>BSHC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative cysteine ligase BshC</fullName>
            <ecNumber evidence="1">6.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bshC</name>
        <name type="ordered locus">STH2589</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in bacillithiol (BSH) biosynthesis. May catalyze the last step of the pathway, the addition of cysteine to glucosamine malate (GlcN-Mal) to generate BSH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BshC family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="BAD41574" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.-.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41574.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_043714106.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67L72"/>
    <dbReference type="SMR" id="Q67L72"/>
    <dbReference type="STRING" id="292459.STH2589"/>
    <dbReference type="EnsemblBacteria" id="BAD41574">
        <property type="protein sequence ID" value="BAD41574"/>
        <property type="gene ID" value="STH2589"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2589"/>
    <dbReference type="eggNOG" id="COG4365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022249_1_0_9"/>
    <dbReference type="OMA" id="TTGHQLN"/>
    <dbReference type="OrthoDB" id="295429at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01867">
        <property type="entry name" value="BshC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011199">
        <property type="entry name" value="Bacillithiol_biosynth_BshC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10079">
        <property type="entry name" value="BshC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012535">
        <property type="entry name" value="UCP012535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03998">
        <property type="entry name" value="thiol_BshC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000378271" description="Putative cysteine ligase BshC">
        <location>
            <begin position="1"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="468"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="556" mass="62442" checksum="38C9C55FE74FEAC6" modified="2009-06-16" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L74</accession>
    <name>Q67L74_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-type D-Ala-D-Ala carboxypeptidase</fullName>
            <ecNumber evidence="4">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH2587</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S11 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2587"/>
    <dbReference type="EnsemblBacteria" id="BAD41572">
        <property type="protein sequence ID" value="BAD41572"/>
        <property type="gene ID" value="STH2587"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2587"/>
    <dbReference type="eggNOG" id="COG1686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027070_8_1_9"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012907">
        <property type="entry name" value="Peptidase_S11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07943">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00936">
        <property type="entry name" value="PBP5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBP5_C" evidence="15">
        <location>
            <begin position="264"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="12">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD41572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="374" mass="40752" checksum="B38027494C02B1EB" modified="2004-10-11" version="1">MLLDFATGQVLYEKNPDEPIAPASLTKLMTLHLAFKRIEAGQMSYDDPVVVSRNAWAANFPDSSLMFLEPGDKVTVGEVMKGLAIVSGNDAAVALAEHIGGTVENFVKMMNDEAQALGFKTFRFVDPHGLSPQNQVTAGEFARFARLYIQLHPESLEMLHSQKEYSYPQYENLSDARQAVTSPEAHEPITQMNRNGLLWTYEGVDGLKTGFVDEAGFNLAVTAQRGGMRLVGVLLGIQADSIPEGSAKREAEGAALLSWGFNNFVTVKPQMPEVKPVRVWKGAANEVTLQAAGGTPTLVLEKGLESSLTASVRQEESVTAPVAKGQKLGEVIFAADGQEVARLDLVAAEDVPQGGFFKRIWDSLKLWIHGFFNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L76</accession>
    <name>ACKA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetate kinase</fullName>
            <ecNumber evidence="1">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ackA</name>
        <name type="ordered locus">STH2585</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196707.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67L76"/>
    <dbReference type="SMR" id="Q67L76"/>
    <dbReference type="STRING" id="292459.STH2585"/>
    <dbReference type="EnsemblBacteria" id="BAD41570">
        <property type="protein sequence ID" value="BAD41570"/>
        <property type="gene ID" value="STH2585"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2585"/>
    <dbReference type="eggNOG" id="COG0282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020352_0_1_9"/>
    <dbReference type="OMA" id="KIITCHI"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107629" description="Acetate kinase">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="282"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="330"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <sequence length="398" mass="43191" checksum="E018C7C53B3D3B06" modified="2004-10-11" version="1">MKILVLNCGSSSIKYQLLDMETESVLAIGKVERVGMEDAIFEYKGAGEKIKEIGTILDHTAGVRKVLKALTDPVHGVIQAPEEIAAVGHRVVHGGEAFVKSVIITDEVKRTLRELFDLAPLHNPANLTGILAAEAALPHAPGVAVFDTAFHATMPRKAFLYALPYAMYKGHKVRRYGFHGTSHKYVSQRAADLLGRPLEEVNLITCHLGNGSSVTAVQGGKSVDTSMGFTPLAGLIMGTRAGDLDPGVIPYIMAREEIGISEVNSMLNKHSGILGISGISSDMRQIEEEMEKGDPRAVETFEMMEYRLRKYIGAYAAVLGRVDGIVFTGGIGENSPLFRKAVCQNLGFLGVTFDEEANNCRGQERIISGPDSKVAVMVIPTNEELMIARETAELIRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L82</accession>
    <name>Q67L82_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41564">
        <property type="protein sequence ID" value="BAD41564"/>
        <property type="gene ID" value="STH2579"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2579"/>
    <dbReference type="HOGENOM" id="CLU_1634531_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="162" mass="17053" checksum="58002C302DFEB604" modified="2004-10-11" version="1">MLAKRSLSSVGRARALHAWGRGFESLSDHQQPQGFTGSGSLAGACALTRLVGRVPRCGADPMAAWKKASTFRAASSHSLSSACADRFAVIVTVERPRAFCVTVSETPSRPGVGTAAEGCGMALIVTLPVPLRVRCRCFPPALVPSRGPSQPTLRPELRCLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L95</accession>
    <name>Q67L95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196688.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2566"/>
    <dbReference type="EnsemblBacteria" id="BAD41551">
        <property type="protein sequence ID" value="BAD41551"/>
        <property type="gene ID" value="STH2566"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2566"/>
    <dbReference type="HOGENOM" id="CLU_2669747_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="7498" checksum="C2142C9F7D634367" modified="2004-10-11" version="1">MSRKGHGKRGSSAVWAVTAAAVALVAVLICASVWSARSGRAPAGEAVRDADLGAVGNAIGPADAPVTVVEYMDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA6</accession>
    <name>Q67LA6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TGc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2555"/>
    <dbReference type="EnsemblBacteria" id="BAD41540">
        <property type="protein sequence ID" value="BAD41540"/>
        <property type="gene ID" value="STH2555"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2555"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328154_0_0_9"/>
    <dbReference type="OMA" id="TPEMARY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025403">
        <property type="entry name" value="DUF4129"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021878">
        <property type="entry name" value="TgpA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13559">
        <property type="entry name" value="DUF4129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11992">
        <property type="entry name" value="TgpA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="TGc" evidence="3">
        <location>
            <begin position="444"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="721" mass="78541" checksum="0F73D83A9F0E852D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA8</accession>
    <name>Q67LA8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Methanol dehydrogenase regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moxR1</name>
        <name evidence="3" type="ordered locus">STH2553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196676.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2553"/>
    <dbReference type="EnsemblBacteria" id="BAD41538">
        <property type="protein sequence ID" value="BAD41538"/>
        <property type="gene ID" value="STH2553"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2553"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_9"/>
    <dbReference type="OMA" id="DRFMFAI"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="37"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="228"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="319" mass="35420" checksum="DB0BBF52F442204F" modified="2004-10-11" version="1">MNAKEAVSRVIGNIELVVVGKRREIQYVLVALLCQGHVLIEDVPGVGKTTLVRSLARSLGCEFRRIQFTPDLLPSDVTGVSIYNQKTGEFEFRPGPIMAQIILADEINRTSPKTQSALLECMEEGQLTVDGVTRRLPRPFLVLATQNPIEYEGTFPLPEAQLDRFLLKLRLGYPSLRDEMTVVERSRRPPLEELQQVLTADEVLRLQQAVKDVHVDESVLEYIVRLVQATREHPDVYLGASPRGSIALYRTSQALALMRGRSYVTPDDVKEMAPLVLSHRIILRPEAQLRSGGAEALVSALLHQVPVPAGVSGHAARRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LE9</accession>
    <name>Q67LE9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41497">
        <property type="protein sequence ID" value="BAD41497"/>
        <property type="gene ID" value="STH2512"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2512"/>
    <dbReference type="HOGENOM" id="CLU_2425879_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="91" mass="10466" checksum="3607119EF8EC46E5" modified="2004-10-11" version="1">MRVTVMTGNRKDAQPSARERARRYRVRRVRRRIEPGEERDVRGPAEAADDADPGTPEQDRALQSGCMSMVRVVGLFFVVMILSIIATWFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK8</accession>
    <name>Q67LK8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spore germination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gerXB</name>
        <name evidence="7" type="ordered locus">STH2453</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196576.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2453"/>
    <dbReference type="EnsemblBacteria" id="BAD41438">
        <property type="protein sequence ID" value="BAD41438"/>
        <property type="gene ID" value="STH2453"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2453"/>
    <dbReference type="eggNOG" id="COG0531">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047547_1_0_9"/>
    <dbReference type="OMA" id="YLVEWIM"/>
    <dbReference type="OrthoDB" id="842200at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004761">
        <property type="entry name" value="Spore_GerAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34975">
        <property type="entry name" value="PTHR34975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03845">
        <property type="entry name" value="Spore_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00912">
        <property type="entry name" value="2A0309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="366" mass="39194" checksum="D88D53C3B93AF1BA" modified="2004-10-11" version="1">MQQFEGHISAREGSVLLFTVLGAMLFLQYPQYLVRIGGPAAWQAGILITLFSLLAVLPMVVLVRRFPGESLADISLQTAGPVVGPLLTLAVSVWLLAVSIVTLRNFTDTFINTILPQTPPSVLIVTATAAAVFSSYRGAEAIARTAYILLPLIAAGVLSVLVFSLPRMDTTLLFPLWGFGFRQTLLGSLYLTSVAAEAIALLAVGNVFRTARCLQHSTLQGILLFGVTATFTVTVLVATAGPPVAREAPFPVYYLARLIYLGRFLQRTESLIVLLWMFAAGTRFSALFHAGVASLSGALRLPEYRPLLFPCAVLVSALSLLPQDYVTVIRLDRLVIRPMGFGALAVPLLLLILAAIRGKGAAGNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL5</accession>
    <name>Q67LL5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196569.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2446"/>
    <dbReference type="EnsemblBacteria" id="BAD41431">
        <property type="protein sequence ID" value="BAD41431"/>
        <property type="gene ID" value="STH2446"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2446"/>
    <dbReference type="eggNOG" id="COG0125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160060_0_0_9"/>
    <dbReference type="OMA" id="LRWFIRS"/>
    <dbReference type="OrthoDB" id="2016147at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="125" mass="14533" checksum="468EA75948A12BAF" modified="2004-10-11" version="1">MRLREPVVAIVGVCAAGKTTLAAGLNAAGIRAYSVPQEHSVVRRLWKHLHPDANILVMLDARWETARRRRPEIRYGPERLEEQRRRLAYARAECDLYLPTDDLSIEDVRQRVLAFIETWKEKERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS7</accession>
    <name>Q67LS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196507.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41369">
        <property type="protein sequence ID" value="BAD41369"/>
        <property type="gene ID" value="STH2384"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2384"/>
    <dbReference type="HOGENOM" id="CLU_2482154_0_0_9"/>
    <dbReference type="OrthoDB" id="1264653at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="87" mass="9708" checksum="0F9BB272A6591977" modified="2004-10-11" version="1">MEEILRGYRDRAYALLAENRAQSREGIELLVRERLPHVAAAMQAGGKPLTTAQLDALYHELLEISFTMFSLGYTMAHTVDQTAGTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW3</accession>
    <name>Q67LW3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196471.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2348"/>
    <dbReference type="EnsemblBacteria" id="BAD41333">
        <property type="protein sequence ID" value="BAD41333"/>
        <property type="gene ID" value="STH2348"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2348"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_8_2_9"/>
    <dbReference type="OMA" id="LDMREGQ"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="260" mass="28306" checksum="F6282FC45D2B128D" modified="2004-10-11" version="1">MPGGAAIHPRPLPLWQQVVDMIQRNVEEGHWPVGHRIPSERELCQQHGVSRTTVRLAIAEAVSRGLLHRVHGKGTFVARPKIHQPLVQVTPFAAALRAQGLVPRTRLLAVMTQPADPATAHMLGLPTGQEIMRFRLLGLGSGEPVAAYHSTVPAALGEAVLERLEADAGADRFRLVVDVLAETFGWSHLTAEQTFEAAGASAEEARLLGLPRGAPVLVVTSLVRNPAGRTVEFARAVYRADQYRFHLTRQIEVSGQDHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LW6</accession>
    <name>Q67LW6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Micrococcal nuclease-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2345"/>
    <dbReference type="EnsemblBacteria" id="BAD41330">
        <property type="protein sequence ID" value="BAD41330"/>
        <property type="gene ID" value="STH2345"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2345"/>
    <dbReference type="eggNOG" id="COG1525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046484_5_0_9"/>
    <dbReference type="OMA" id="ISKGTRW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01123">
        <property type="entry name" value="TNASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="2">
        <location>
            <begin position="72"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="282" mass="29120" checksum="62F9FDF8BB59FC38" modified="2004-10-11" version="1">MQAGAGQDGTRTGTEAPDTTDATVGAEPASGAPHADAGQGAPSSDGTAGAAAGREEGASAGEAQSPAPTPAQTLQATVQRVVDGDTVDVLVDGREERVRLIGVDTPEVYGKAEAYGAEASAFTKQRLTGQEVRLELDVEERDRYGRLLAYVWIGDEMFNETLVREGYAQVMTVPPNVKYADRFVALQREAREHGRGLWGLEEASGSGAGVAASGGTASGGAAGGSVLPVNGDCGGLIKGNVSSSGELIYHVPGGQFYDRTRAEACFATPEEAEAAGYRRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LY4</accession>
    <name>Q67LY4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitro_FeMo-Co domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196450.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2327"/>
    <dbReference type="EnsemblBacteria" id="BAD41312">
        <property type="protein sequence ID" value="BAD41312"/>
        <property type="gene ID" value="STH2327"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2327"/>
    <dbReference type="eggNOG" id="COG1433">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_2_0_9"/>
    <dbReference type="OMA" id="AGCGCKS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="2">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="127" mass="13396" checksum="8577FA2474D12ACD" modified="2004-10-11" version="1">MRIAVPVQEDRLNPHFGRSPAFAFFDVDPGTRQITGPQILPAPPHDHGALAAFIKENGAAVVIAGGMGPGMRHALDREGITVVLGAPGIDPEEVVRQYLDGTLVTADPGRGHDHGQGCCHRHAGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ1</accession>
    <name>Q67LZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LuxR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2320"/>
    <dbReference type="EnsemblBacteria" id="BAD41305">
        <property type="protein sequence ID" value="BAD41305"/>
        <property type="gene ID" value="STH2320"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2320"/>
    <dbReference type="eggNOG" id="COG2771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_726982_0_0_9"/>
    <dbReference type="OMA" id="WRIAVEN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018656">
        <property type="entry name" value="DUF2087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09860">
        <property type="entry name" value="DUF2087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2087" evidence="2">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="43383" checksum="E4FE5C70C9257D36" modified="2004-10-11" version="1">MSPEALLNAATLADLKRGYLESTDAYTCLCCGERVEKGVVYPVGDRLLEAGRYIQQHIAEAHGSVFAHLVALDKQAHGLSDLQRRLLTLFYEGKSDEEIKALLGAGSLSTIRNHRFILREKERQARIFLAIMELLHERQSTGGATGAHGGKRSRSGKAPADQRSADDAILARYFPYGPEGPVQHFSTAPLKHKRMIAAVVAQRFTPGRTYSEREVNAVLEPIVEDYVLLRRCLVEFGYLKRLPDGSQYWRGDVDEEGMKQVDRRKELKRLAREAKTEGGVYQVRNTRNGMVWIGVTPNFRTLNGQRFQLEMGSHRSRELQQAWNEYGPDAFVFEPLEALEVPETGPFDRDAALRKLKQKWLRELQPFGSRGYNLPSELEEETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M11</accession>
    <name>Q67M11_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2299</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the four-carbon acid sugar kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2299"/>
    <dbReference type="EnsemblBacteria" id="BAD41284">
        <property type="protein sequence ID" value="BAD41284"/>
        <property type="gene ID" value="STH2299"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2299"/>
    <dbReference type="eggNOG" id="COG3395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029424_0_1_9"/>
    <dbReference type="OMA" id="IASCVPW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037051">
        <property type="entry name" value="4-carb_acid_sugar_kinase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010737">
        <property type="entry name" value="DUF1537"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031475">
        <property type="entry name" value="NBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042213">
        <property type="entry name" value="NBD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17042">
        <property type="entry name" value="NBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07005">
        <property type="entry name" value="SBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SBD_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="NBD_C" evidence="8">
        <location>
            <begin position="249"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="417" mass="42695" checksum="7835732BE04A56EE" modified="2004-10-11" version="1">MTSSEHRPAIGVIADDLTGANATGVLLTRTGYPTASLTELRWPEGGLDGYPCIVIATESRAVPADEARRRVADAARLLLDHGRRPMAKRIDSTLRGNLGPEVEAVLEALGPGSVAVLTGAFPASGRTARDGIHYVHGVPLAETEVRHDPLCPVTQSHVPTLVGSQTGLPVAHLPLAAVRAGAEAVARAIAEQARQGVRILCADAETDADIQALGQGMALSGVTCVAADPGPLTAAFVAASVPPVRGRVLVVAGSVTDLTRTQLDHLQAETGARLIQVDAGSLGRGGEDAEAEVRRAVDALAAIPAGEPVIGVRSSEVLSVDPDAAGRIAAGFAEIAARALDRLEGVKGLYTSGGDITVAVCRRLQAGAIQLDAEVLPLAVQGRLVGGLRPGLAIVTKGGLVGDRTAAVTCVRHLIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M62</accession>
    <name>Q67M62_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2248"/>
    <dbReference type="EnsemblBacteria" id="BAD41233">
        <property type="protein sequence ID" value="BAD41233"/>
        <property type="gene ID" value="STH2248"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2248"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2669744_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="7821" checksum="BA29DBFD64FF49F1" modified="2004-10-11" version="1">MVPSPAVVAGDWITKGGSPQRTSTVLDPHGLVELSPYWETEALGESAAQPAVVDGVIFHLAGPTSGGWSWMTSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M70</accession>
    <name>Q67M70_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2240"/>
    <dbReference type="EnsemblBacteria" id="BAD41225">
        <property type="protein sequence ID" value="BAD41225"/>
        <property type="gene ID" value="STH2240"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2240"/>
    <dbReference type="HOGENOM" id="CLU_2921096_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="61" mass="6741" checksum="ABC0EEC1FD4CF697" modified="2004-10-11" version="1">MILGVTSWLSRIAPTTQGRKIGPQVARSALVRMERFGWCKVWIVKMDIQAGGSTKLQPPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M76</accession>
    <name>Q67M76_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoglycerate dehydrogenase, N-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2232"/>
    <dbReference type="EnsemblBacteria" id="BAD41217">
        <property type="protein sequence ID" value="BAD41217"/>
        <property type="gene ID" value="STH2232"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2232"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1834164_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="1">
        <location>
            <begin position="7"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="140" mass="15061" checksum="8688C89249CC2E83" modified="2004-10-11" version="1">MADRIKVLMLQAIHEEGILLFDDRFEVIAARDPSPAAVIPELDGVQAIIVRLAPCTREIIEAAPDLRVIAKHGVGVDNIDVAAATERGILVLNTPEANAVSVAEHAIAAIAALAKRVVFMDRAVRDGRWQARGDLKAIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M77</accession>
    <name>Q67M77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoglycerate dehydrogenase, C-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2231"/>
    <dbReference type="EnsemblBacteria" id="BAD41216">
        <property type="protein sequence ID" value="BAD41216"/>
        <property type="gene ID" value="STH2231"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2231"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_4_9"/>
    <dbReference type="OMA" id="HRIPYRY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh_C" evidence="3">
        <location>
            <begin position="2"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="191" mass="20569" checksum="12163868882A5C73" modified="2004-10-11" version="1">MLGLVGIGRIGTQVARKAQAAFDMQVIGYDPYVAPEAAQAHGIRMVADLAELFRTADVVSIHAPLTPETRGLVTRELIGMMKPTAFLVNFARGEIVDEGALVEALREERIAGAALDVFEREPVDPENPLLQLDNVLLSPHSAAQTRECVIRMSVTTAQGVIDALTGRRPRYIVNPEVLLRSGRAGEGEMGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M83</accession>
    <name>Q67M83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196350.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2225"/>
    <dbReference type="EnsemblBacteria" id="BAD41210">
        <property type="protein sequence ID" value="BAD41210"/>
        <property type="gene ID" value="STH2225"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2225"/>
    <dbReference type="HOGENOM" id="CLU_653689_0_0_9"/>
    <dbReference type="OMA" id="MHEREAF"/>
    <dbReference type="OrthoDB" id="1936828at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013494">
        <property type="entry name" value="CHP02678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09661">
        <property type="entry name" value="DUF2398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02678">
        <property type="entry name" value="TIGR02678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="420" mass="47319" checksum="E1833C5F56402057" modified="2004-10-11" version="1">MATTGAAEQRQLALQDLMDRYLISQADEPEAYRRVALHEGYLKAWFHERPRWRILSGRGVYRLERMPSQVLFHRGLPRLRSPLSYACLCWVLWFAETLVTTARDWFVISELAQRIATVSEGRFTLAERSHREALVQALQFLIDLGGLLLRDGDADRWVAGQDYLGEPPEVMYEFTEMAPRLLANFSYESLAVAVTADHGRRTAPPTGEEAPPLTRAWRALLLGPVFWKADDPEAFAALEANYEAVYRDLEASLGWQVELTSSFARIWRTTTARHAGAVLLDLYPDPGEEAEERHTRYLFHPILLLLGRCQEGVAAGRWSADADGAVAISAGELEDLLTELRSQHRPSWGAALGALSIAELVTVVLSEMRRMGLLRGPDRFGRCWLLPAAAGIRGRYVTRETGARRAPEPEQPQAQQIRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MA7</accession>
    <name>Q67MA7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2201"/>
    <dbReference type="EnsemblBacteria" id="BAD41186">
        <property type="protein sequence ID" value="BAD41186"/>
        <property type="gene ID" value="STH2201"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2201"/>
    <dbReference type="HOGENOM" id="CLU_1642873_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="17269" checksum="D2B9F62E2A39EAD2" modified="2004-10-11" version="1">MCTAIGLMAFSLVITFIRMRYSVMIRGSAAPTNVRFSITMVTFLYMVITQLPGIRDKVDWKRPLGRTGPHSTPGGLALMVAGLFTAISPWGVGWTHVFDGVNYALLMAKPLAITGGLLMLAGAGLLLSARLGRPPGEWLADGVRWRIAARPPETAAKGGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MP8</accession>
    <name>Q67MP8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutaredoxin-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2060"/>
    <dbReference type="EnsemblBacteria" id="BAD41045">
        <property type="protein sequence ID" value="BAD41045"/>
        <property type="gene ID" value="STH2060"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2060"/>
    <dbReference type="eggNOG" id="COG3634">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1785917_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="145" mass="15623" checksum="265F562310EE6F3A" modified="2004-10-11" version="1">MVALSAAQRRQVSSLFAEHLRAPVQMVFFSATEHDRSTAALRALLADLASLSDGRIRVVARSGPEARAEAAAYGVKQAPALVLLDGAGRDTRIRFYGVPMGVDLVVLLEDLIDVSHGETRLSPPARALVRTVDRDLTVQVYSTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR8</accession>
    <name>Q67MR8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196166.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2040"/>
    <dbReference type="EnsemblBacteria" id="BAD41025">
        <property type="protein sequence ID" value="BAD41025"/>
        <property type="gene ID" value="STH2040"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2040"/>
    <dbReference type="eggNOG" id="COG2151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_2_2_9"/>
    <dbReference type="OMA" id="PPWGPER"/>
    <dbReference type="OrthoDB" id="1668114at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="11775" checksum="28F0F6C961FE3332" modified="2004-10-11" version="1">MAEITKEQVLKVLERVNDPELRVNIVDLGLVYDVEITEDNNVGIDMTLTYPGCPIGPLIQMQVEAALKSIPGVNQVDVRFVWDPPWTPDRMSERLKKARQMGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT3</accession>
    <name>Q67MT3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HtrA family serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2025"/>
    <dbReference type="EnsemblBacteria" id="BAD41010">
        <property type="protein sequence ID" value="BAD41010"/>
        <property type="gene ID" value="STH2025"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2025"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_2_2_9"/>
    <dbReference type="OMA" id="PPAIHIQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="198"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="30935" checksum="37AA2D5DD998C949" modified="2004-10-11" version="1">MFQMVGEREPFARVASQVGPGVVQVRAGRSQGSGVLLDPHSVITNAHVVGDSPSPAITFADGVTRPGRVVAADRLYDLALVETVSPVNARIEPAPEESVVPGRQVVAIGNPFGFGWTVTTGVVSAVDRIVGGLDGLIQTDAAINPGNSGGALVDLDGRLVGIPTLVLAPGQNIGFAIPAWQVLHVVEQFRRTGRAEHPYVGLAGATEVIDPVVARALDLPGRGVLVAGVEPGGPAHLAGLGEWDMVIAVDGAPTPSVQALRRQIRRTPVGATVPFTVLRGGRLLQVPVRVGELPAAARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX3</accession>
    <name>Q67MX3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Anaerobic glycerol-3-phosphate dehydrogenase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1985</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1985"/>
    <dbReference type="EnsemblBacteria" id="BAD40970">
        <property type="protein sequence ID" value="BAD40970"/>
        <property type="gene ID" value="STH1985"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1985"/>
    <dbReference type="eggNOG" id="COG3075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047793_1_0_9"/>
    <dbReference type="OMA" id="RYEWFVD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009158">
        <property type="entry name" value="G3P_DH_GlpB_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000141">
        <property type="entry name" value="Anaerobic_G3P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03378">
        <property type="entry name" value="glycerol3P_GlpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="4">
        <location>
            <begin position="2"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="399" mass="40692" checksum="0B6C38EE26B93698" modified="2004-10-11" version="1">MLVIGGGLAGMAAALAARQAGATVAVVSEGAGVLELASGCIDRLAVPEGSLPPEHPYRLIGMAAVDEELAAFQKAMEAAGYPLAADGPGAGGSHVITALGSRRRTHLVAPGMAAPPPGEPLWVVGFRGLRDFHPAVVAAGLRRSDPAADVAWGEVELPGAPEDVHPVALARQLEDEAYRRQVIERVAAVRPQGRGGAALLPAVLGLSGAAAVRRALAEGLGMRVVEVLLPPPSVPGLRLADALRRAVQQAGVDLAIGARAVEARREGGRVRHVVTRGPGGRITYEAEAYVLATGGLMGNGLTAEGRSVREPLFDLPVSAPDGEWADPRFLPAEGHPFVRVGVAVDGRLRPPGYTNLFVCGRALAGYDPYAEGSGGGVAVATGGAAGREAARLVAGGEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N31</accession>
    <name>Q67N31_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cobD</name>
        <name evidence="11" type="ordered locus">STH1927</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196054.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1927"/>
    <dbReference type="EnsemblBacteria" id="BAD40912">
        <property type="protein sequence ID" value="BAD40912"/>
        <property type="gene ID" value="STH1927"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1927"/>
    <dbReference type="eggNOG" id="COG1270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_1_0_9"/>
    <dbReference type="OMA" id="WGYRNER"/>
    <dbReference type="OrthoDB" id="2029688at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="33387" checksum="B3E90DC64C6DFAF3" modified="2004-10-11" version="1">MFPAAPWYLPLAALAVDAAVGDPARLPHPVALMGRVIAWAEPRLRRTRLPLRAAGVLLALLLPVAAWGLTWGLIRLAAALHPWLGVAAEVWLLSTCLAARSLADHALAVYRPLKAGDLSEARRRVAHIVGRDTAGLDAAEVTRATVETVAESTCDGVIAPLFWGLVGGAPLAMAYKAANTLDSMVGHRNERYLEFGWASARLDDLVNLAPARLSALLLALAGLSPKALRIALRDAPRHPSPNSGWPEAAMAALLRVQLGGLNHYGGRPERRAHMGDPLEMLQPGHIVQAVRWMWLATLLGTGLGALTLWFLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-06-28" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N40</accession>
    <name>LIPM_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Octanoyltransferase LipM</fullName>
            <ecNumber evidence="1">2.3.1.181</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Octanoyl-[acyl-carrier-protein]:[GcvH] N-octanoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipM</name>
        <name type="ordered locus">STH1918</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domain of GcvH, an intermediate carrier during protein lipoylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. The reaction proceeds via an octanoyl-thioester enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the octanoyltransferase LipM family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196045.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N40"/>
    <dbReference type="SMR" id="Q67N40"/>
    <dbReference type="STRING" id="292459.STH1918"/>
    <dbReference type="EnsemblBacteria" id="BAD40903">
        <property type="protein sequence ID" value="BAD40903"/>
        <property type="gene ID" value="STH1918"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1918"/>
    <dbReference type="eggNOG" id="COG0095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022986_5_0_9"/>
    <dbReference type="OMA" id="YAYLDDW"/>
    <dbReference type="OrthoDB" id="871298at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009107">
        <property type="term" value="P:lipoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02118">
        <property type="entry name" value="LipM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024898">
        <property type="entry name" value="LipM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000410865" description="Octanoyltransferase LipM">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="32"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01067"/>
        </source>
    </evidence>
    <sequence length="274" mass="30049" checksum="9EDA5B5053416A1E" modified="2004-10-11" version="1">METNWRLLDTGHRPGPENMAIDEAIAMAHGRGEVPPTLRFYGWNPPAVSIGYFQSMLGEVDLDAVRAGGYGYVRRPTGGRLIFHHMELTYSVVIREELLPGGVIETYREISRGLLAGMAELGVPAALSGGDRDPRRADPDGFHTACFDTASAYELQVGGRKVAGSAQTRRDGVILQHGSILLDIDVPLLFRLMRLPEGIPAERLMARFRAKSTTLAEALGRPVSWAEARDAFAAGFARALGLTLTPGQLTEREEKEAQTLVEAKYGCDNWNMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N42</accession>
    <name>Q67N42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1916"/>
    <dbReference type="EnsemblBacteria" id="BAD40901">
        <property type="protein sequence ID" value="BAD40901"/>
        <property type="gene ID" value="STH1916"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1916"/>
    <dbReference type="eggNOG" id="COG1514">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1371597_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="199" mass="20616" checksum="D6C5ACF8A4318709" modified="2004-10-11" version="1">MDFTHPTYVILPIKPPVADQVLRIRERYGHPSPNPVEVTVAGSSGVDVLEPDQDPAAVVQALERIAASTPPFQTEFGEVRRFPGTHIYWLSLKDEAPFRAAEELRHSLPAQPLPLPAPLHAAGAGALRGGGGGAPPPASSGHGGLRRDRPGGGGGAGEPAGGGPHPVDGQAHRRMSPQGHASQADPPRGSAWLHPCACG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N45</accession>
    <name>Q67N45_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative amidohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1913</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196040.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1913"/>
    <dbReference type="EnsemblBacteria" id="BAD40898">
        <property type="protein sequence ID" value="BAD40898"/>
        <property type="gene ID" value="STH1913"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1913"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046987_0_0_9"/>
    <dbReference type="OMA" id="PRIKMEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="3">
        <location>
            <begin position="307"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="382"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="42462" checksum="420AA752087B2851" modified="2004-10-11" version="1">MLAVVNARILTVSAGVIPSGAVLVDDAGRIAAVGERVDVPEGTEMIDAGGRTLTPGLVDGFSRLGLINEGLGWAGSDADESTDPNTAHVRALDGLYPFDRGIAEAHTGGVTTVHVVPGGSNVLAGQALAIKLRRAVAADDLVLRHPTGVAAGLGERPKAAYGGEKRMPATRMGVAAVLREALVKAQEYDPGKERDLRCEALLPVLRGELPLLVEAHRADDIVTALRIGREFRIRITLLGATEGHLVADLIAEAGAAVAAGPSLAARGRQETRDVGFHLPLALHRHGVPTAIVTGHPQLDQLYLNVAAALAVREGLMPEEALRAVTLTPAEILGVADRVGSIAPGRDADLVLWDGDPLELTTRVAWTMVEGRVVYMDDDRGSAHGGWGHDGREGPEPGRPCGEVNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N92</accession>
    <name>Q67N92_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="7">3-dehydroquinase</shortName>
            <ecNumber evidence="5 7">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DHQase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroQ</name>
        <name evidence="11" type="ordered locus">STH1866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes a trans-dehydration via an enolate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Homododecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="5 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195994.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1866"/>
    <dbReference type="EnsemblBacteria" id="BAD40851">
        <property type="protein sequence ID" value="BAD40851"/>
        <property type="gene ID" value="STH1866"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1866"/>
    <dbReference type="eggNOG" id="COG0757">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090968_1_0_9"/>
    <dbReference type="OMA" id="AYTHYSY"/>
    <dbReference type="OrthoDB" id="1872106at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="PTHR21272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="SSF52304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 9">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7 8">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7 10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="16462" checksum="4DD0AA31E2ED984F" modified="2004-10-11" version="1">MVRILVIHGPNLNLLGTREPEVYGSTTLSEIDAMLSELAGELGVEVESFQSNHEGAIIDAIHDARGRFDGILLNPGAYTHYSLAIRDAVAAVGLPVVEVHLSNIYQREPFRHRSVIAPVAAGTIAGFGAESYRLGLRALAHLLQGDRAQRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NH1</accession>
    <name>Q67NH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore maturation protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195915.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1787"/>
    <dbReference type="EnsemblBacteria" id="BAD40772">
        <property type="protein sequence ID" value="BAD40772"/>
        <property type="gene ID" value="STH1787"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1787"/>
    <dbReference type="eggNOG" id="COG2715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089992_1_0_9"/>
    <dbReference type="OMA" id="ASDAMCT"/>
    <dbReference type="OrthoDB" id="1316897at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="43"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="196" mass="20183" checksum="6AC1F7630E6EA36D" modified="2004-10-11" version="1">MLNMIWLGLILVGVAVAGATGRAAEVTDAAFSGGKLAIETAIGLAGTMALWMGIMKIAERSGLMEGLAALLRPVVGWLFPSVPRDHPAVGAMLMNISANMLGLGSAATPFGLKAMQELQELNPEKETASEAMCTFLALNTASITLVPGTVIALRATYGSVNPAETVAATLVASAVAGVFSIGLDWAVRMSLRARGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NQ7</accession>
    <name>Q67NQ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195829.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1701"/>
    <dbReference type="EnsemblBacteria" id="BAD40686">
        <property type="protein sequence ID" value="BAD40686"/>
        <property type="gene ID" value="STH1701"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1701"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1239615_0_0_9"/>
    <dbReference type="OMA" id="RIANYLM"/>
    <dbReference type="OrthoDB" id="1466627at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="25744" checksum="F22A337F9D7C179B" modified="2004-10-11" version="1">MPFYGKLFLFCRAVLRRLSPRYTLVHPERLLEPAVYLCRHADIHGIVLNLLWLPVPVRTWGLHQFGDRGACYRHLRDITFGARYGWPRWKATALAWPASIGLSALYRSAGCIPVYRGSSQIIRTFRESTEALKRGESLLIFPDKDYASTDSEVSELYEGFLSIDRFYHRATGRHIPFVPLYIDRQARTIRVCEPLFFSDGRDRKAQTAELIRTLQERLGGHGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT9</accession>
    <name>Q67NT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195798.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1669"/>
    <dbReference type="EnsemblBacteria" id="BAD40654">
        <property type="protein sequence ID" value="BAD40654"/>
        <property type="gene ID" value="STH1669"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1669"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_4_5_9"/>
    <dbReference type="OMA" id="RPWVEGL"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="35"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="22589" checksum="651FA1D963FF9966" modified="2004-10-11" version="1">MWLYGFARIVVGLGYRLAYRVQAEGREHIPPTGGVILCGNHINAQDPIVIGLACPRPICFMAKEELFRSQLLGFIIKGLGAFPVKRGSADRAALKHALALLEAGCCFGIFPEGTRSRTGELQKPEPGTAYIALKSGVPVIPVGISGSYRLFSPIRVRFGPPVDLDRFRGEKLNGETLEAASQAIADAIAALLDPPSGLSNAGAGPPAGRALSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NW6</accession>
    <name>Q67NW6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spore germination protein KA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gerKA2</name>
        <name evidence="4" type="ordered locus">STH1642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1642"/>
    <dbReference type="EnsemblBacteria" id="BAD40627">
        <property type="protein sequence ID" value="BAD40627"/>
        <property type="gene ID" value="STH1642"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1642"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021639_4_0_9"/>
    <dbReference type="OMA" id="IFREFRI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004995">
        <property type="entry name" value="Spore_Ger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03323">
        <property type="entry name" value="GerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005690">
        <property type="entry name" value="GerBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="431"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="567" mass="62055" checksum="36378A4C91907ED4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX0</accession>
    <name>Q67NX0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cell division protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1638</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1638"/>
    <dbReference type="EnsemblBacteria" id="BAD40623">
        <property type="protein sequence ID" value="BAD40623"/>
        <property type="gene ID" value="STH1638"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1638"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_9"/>
    <dbReference type="OMA" id="HMEYDQT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="8">
        <location>
            <begin position="85"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="493" mass="53801" checksum="486EBA176B425EF0" modified="2004-10-11" version="1">MLAYLAITGVNVLPVVFLAGLVWLMYQSSVLRGPSAAAVRSPATTAVPQVRFEDIGGQAAAKKELLEAIEFIANREQIARMGIRPLKGILLTGPPGTGKTLLAKAAAHHTDSVFLAAAGSEFVEMYAGVGAQRVRELFRRARELARKERKRSAIIFIDEIEVLGARRGSHSTHMEYDQTLNQLLTEMDGIAVDEEIQVLVMAATNRADMMDPALLRPGRFDRMVNVDLPDKEARLAILRLHTRQKPLGDDVDLEAIARQTFGFSGAHLESLANEAAILALREGLSEVRQRHLVEAVDKVMLGERLDRKPTEEEKRRVAVHEAGHALVGEWVDPGSVATVTVTPRGRAMGYVRTSPSDDQYLYTRSQLEARIQVALAGFLAEELFFGEASTGAAGDFEQATALARHIVHAGLSPLGIVDPANLDKGLEAAMVQRIIADQREMVAWFLAERRPLLQRVADVLVDTEAIDGEAIRRMLADAPPLPPVQGPPLPETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P08</accession>
    <name>Q67P08_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1600"/>
    <dbReference type="EnsemblBacteria" id="BAD40585">
        <property type="protein sequence ID" value="BAD40585"/>
        <property type="gene ID" value="STH1600"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1600"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_561311_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="3">
        <location>
            <begin position="170"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="486" mass="53447" checksum="E5CEBB8E22309EF6" modified="2004-10-11" version="1">MWMSDGLGLPALHYLGEQRLALGARRTVEVPSVRYGRFVWSDSCSLLAVGEGVRGRWSCRVAQGEVLLLCPDEQVWVTSLSDHAGELRMLYFETRGEGHGHSIASWVPPVPVRLPGVEPALQARADAANGGSAPLIDFFRIQAHLYELMAAYLSGARRRPGQYSLLSYAEQVRSQMAAHSEREYSIELIARNSGVSPHRFYEAFRLLTGLTPHKYLSALRLRKALRLLAGDFRSVAEVAHAVGYEDEFYFSRVFKRELGLAPSAFVRQIRARPDVNVPCGDLAIFGLAAPPHDSAEPVPESAASPPACPREEEEAPPGAAWSRRVRKLGQLLGLESVAAHWTAIMERRLCHLKGLVRSRFGDTPFLVVGVDTDGYRVFGTRHPGLGDLLYISGGFTPCRTVHGLREVRVASLDEVAAFGCRTALFLVQGRARPSLPAEWADAVGPEGTRCLAVGWCGVEDAARYEWLVERLTLHLLEPEFAADTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P91</accession>
    <name>Q67P91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195647.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1517"/>
    <dbReference type="EnsemblBacteria" id="BAD40502">
        <property type="protein sequence ID" value="BAD40502"/>
        <property type="gene ID" value="STH1517"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1517"/>
    <dbReference type="HOGENOM" id="CLU_1467501_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="184" mass="20379" checksum="CF882FB376AC7A69" modified="2004-10-11" version="1">MTTETKKTESIQFGLTLIQKILYGVLLFGAAFAVTLLSPLFAGPGGTVFFVFYGILVGVLSWFVSSGWVFSLELTPREIRVRDAGRIVAIPLEKVGMVVRGGRSPFLPAVWLVLRGVDIGRELPAKGVNPVTQEMLTHFQRRNPGKKITFVPIPIIYLRSAGEFVGELKRRIPPLTVDERLGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF6</accession>
    <name>Q67PF6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">Acyl carrier protein</fullName>
            <shortName evidence="7">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acpP</name>
        <name evidence="11" type="ordered locus">STH1452</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 9">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1452"/>
    <dbReference type="EnsemblBacteria" id="BAD40437">
        <property type="protein sequence ID" value="BAD40437"/>
        <property type="gene ID" value="STH1452"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1452"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_1_9"/>
    <dbReference type="OMA" id="CEIPDEQ"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 7 8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="10">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="7 8">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="9285" checksum="DFCADC4A0DACF5DE" modified="2004-10-11" version="1">MNLPMSTDPIFEKVKAIIVDQLSVEEEEVTMEASFIEDLGADSLDIVELIMALEEEFDLEVPDEDAEKLTTVGAAVNYIKEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM2</accession>
    <name>Q67PM2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative cell division control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195516.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1386"/>
    <dbReference type="EnsemblBacteria" id="BAD40371">
        <property type="protein sequence ID" value="BAD40371"/>
        <property type="gene ID" value="STH1386"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1386"/>
    <dbReference type="eggNOG" id="COG0464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_437284_0_0_9"/>
    <dbReference type="OMA" id="YRGKIIW"/>
    <dbReference type="OrthoDB" id="1115436at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="306"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="562" mass="62768" checksum="264E97E6B559D776" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PM4</accession>
    <name>Q67PM4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="3 8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">recG</name>
        <name evidence="11" type="ordered locus">STH1384</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1384"/>
    <dbReference type="EnsemblBacteria" id="BAD40369">
        <property type="protein sequence ID" value="BAD40369"/>
        <property type="gene ID" value="STH1384"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1384"/>
    <dbReference type="eggNOG" id="COG1200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_7_1_9"/>
    <dbReference type="OMA" id="DNGFQAC"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004609">
        <property type="entry name" value="ATP-dep_DNA_helicase_RecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00643">
        <property type="entry name" value="recG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="295"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="479"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD40369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="709" mass="78388" checksum="55BD97E4DF19F42E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PQ3</accession>
    <name>Q67PQ3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ordered locus">STH1355</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195485.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1355"/>
    <dbReference type="EnsemblBacteria" id="BAD40340">
        <property type="protein sequence ID" value="BAD40340"/>
        <property type="gene ID" value="STH1355"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1355"/>
    <dbReference type="eggNOG" id="COG0036">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_9"/>
    <dbReference type="OMA" id="WLQVDGG"/>
    <dbReference type="OrthoDB" id="1697639at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="14">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD40340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="220" mass="23159" checksum="F05A2F3C4D673903" modified="2004-10-11" version="1">MKVAPSLLSADFAALGAELAAIEAAGADWAHLDVMDGHFVPNITFGPPVIAKLRPRSKLFFDAHLMIRHPERYIGDFVKAGADLITVHAEACDHLHRVLGQIREAGVKAGVALNPHTPVSVLAHVLDMVDLVLIMTVNPGFGGQRFIDACLPKVRQARAMLAEAGLADRVEIQVDGGVDPHTAPRCVEAGATVLVAGTAVFGKDDYAAAIAALRGQPSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU9</accession>
    <name>Q67PU9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1309"/>
    <dbReference type="EnsemblBacteria" id="BAD40294">
        <property type="protein sequence ID" value="BAD40294"/>
        <property type="gene ID" value="STH1309"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1309"/>
    <dbReference type="eggNOG" id="COG3472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764886_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="362" mass="40248" checksum="74BB84E29C9CC12E" modified="2004-10-11" version="1">MKGRDEDMGSPLEREFHEAMVSICERATRECGYNPRDLRRMIFDHGGLGAARLLLRTDRVSDGFVRLWELKRLDLTVEAHVLLPRFAALFTEEEHRIARRRLQDYGYPVPEPRPAATRPSTQAGEEAVPPAAEAVATSQMATGGSRHHLQRLVGDNPELLNCLLLSASESLRAFAGGHPHWVWPLATNGYRENRDVTYLDALGLADLTPRLAEFWPSDGPVWDGLAQVSGRHGIRGVLLLEARSHVQEITGPGYQVGRESLERMQQRLEQVKAVLGADPAADWVGKYYQAASRIAHLYFLNILAQVPAWLVNLHFVGDREQSGPQTVAEWEVSFKSLDTALGLPPGHLLAGRIITAFLPVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV6</accession>
    <name>Q67PV6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Endonuclease III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195433.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1302"/>
    <dbReference type="EnsemblBacteria" id="BAD40287">
        <property type="protein sequence ID" value="BAD40287"/>
        <property type="gene ID" value="STH1302"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1302"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_4_9"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="34"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="25724" checksum="7A1DC99E823595EC" modified="2004-10-11" version="1">MDWEGLSRALAARYGSPRHGNPTDPTDCLFYLMLSRKTPIRTAARVYKRLRATVRGWDGLLGLTQVELVRLLWGSGLEEIRAGHLLAVAGLLRDRFGAVTLEPLRQWPDDECLAFLMSLPGMGMKTALCVMLYGLDRPVFPADAHCIRVLKRMGVIDARLAHRPAQRELARIVPGHLGYVLHVNLVAHGQQICTARCPRCAECVVANYCLLGEAAQQAAEGQLPYGESLRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PW9</accession>
    <name>Q67PW9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1289"/>
    <dbReference type="EnsemblBacteria" id="BAD40274">
        <property type="protein sequence ID" value="BAD40274"/>
        <property type="gene ID" value="STH1289"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1289"/>
    <dbReference type="HOGENOM" id="CLU_2557038_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="9401" checksum="E30556BCE614D974" modified="2004-10-11" version="1">MNAENDVRRRTSRLGQRSVRATPWSIRKDLSELTFEFHMGREYRQHLIPLLDQVGAHCTCPVAGLPIAERLHFYGQGCPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q14</accession>
    <name>Q67Q14_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">OMP decarboxylase</fullName>
            <shortName evidence="7">OMPDCase</shortName>
            <shortName evidence="7">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrF</name>
        <name evidence="9" type="ordered locus">STH1244</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the OMP decarboxylase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195375.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1244"/>
    <dbReference type="EnsemblBacteria" id="BAD40229">
        <property type="protein sequence ID" value="BAD40229"/>
        <property type="gene ID" value="STH1244"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1244"/>
    <dbReference type="eggNOG" id="COG0284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060704_1_1_9"/>
    <dbReference type="OMA" id="QSAFFER"/>
    <dbReference type="OrthoDB" id="1181405at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01215">
        <property type="entry name" value="OMPdecase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011995">
        <property type="entry name" value="OMPdecase_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43375">
        <property type="entry name" value="PTHR43375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02127">
        <property type="entry name" value="pyrF_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="317" mass="33378" checksum="64AC5EF44FD21233" modified="2004-10-11" version="1">MDQHFGDRLCAAVTRRRSHAVVGIDPVLSRLPEFIRRDAAARYGKTARGAAAALLAFSRLVIDAVADQVALVKPQSAFYEMYGQWGIAAFWETIRYARSRGLLTIADAKRGDIGSTAEAYAAAFFGHPSPLETWEDPDQWADALTVNPYLGSDGLVPFVRRAGARGTGVFLLVKTSNPSSGELQDQPLFSGESLAQQVAKLADSLGEPLVGRSGYSSVGAVVGATYPEDLKLYRAEMPRAILLVPGYGAQGGTAADVVGAFNADGLGAVVSASRSVIYAYRATDPSPAEAVAAIAGAAEAMNRDINAALRAAGKLAW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q16</accession>
    <name>LSPA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="1">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Signal peptidase II</fullName>
            <shortName evidence="1">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lspA</name>
        <name type="ordered locus">STH1242</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195373.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q16"/>
    <dbReference type="SMR" id="Q67Q16"/>
    <dbReference type="STRING" id="292459.STH1242"/>
    <dbReference type="EnsemblBacteria" id="BAD40227">
        <property type="protein sequence ID" value="BAD40227"/>
        <property type="gene ID" value="STH1242"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1242"/>
    <dbReference type="eggNOG" id="COG0597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083252_3_3_9"/>
    <dbReference type="OMA" id="NRWYFPA"/>
    <dbReference type="OrthoDB" id="1575081at2"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0064">Aspartyl protease</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000076937" description="Lipoprotein signal peptidase">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <sequence length="152" mass="16885" checksum="E72EA890C03745C0" modified="2004-10-11" version="1">MLSIVLAIVAVVVDQVTKWLVATRMALHSEIEIIPGFFSLQYVHNTGAAFGMLRNGRWFFVAVAALAVAGILYYLRQPESRHPLLRVALGLVMGGAVGNMIDRIATGRVVDFLLFYWRDYYFPNFNVADICVTVGVGLLFLHLVLVERKGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q65</accession>
    <name>Q67Q65_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195324.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40178">
        <property type="protein sequence ID" value="BAD40178"/>
        <property type="gene ID" value="STH1193"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1193"/>
    <dbReference type="HOGENOM" id="CLU_1546797_0_0_9"/>
    <dbReference type="OrthoDB" id="1433958at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="19568" checksum="F7C8ACFE645A3813" modified="2004-10-11" version="1">MATVCRVDTLYHYLMGGDESLPGILDKGLLPASAFPESERWQRFESFYRSLYAQWAEPLLGPFRNSGVYFTPIDFRLLPGTYLHRRTRIAVPLSEIPLEQAVLTYELDGRRTVVPLTAEALEEAAALWTADLVRAWYARNPNMSFYYVPQVAVFPDGGIAVHTAWVERAPAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q94</accession>
    <name>Q67Q94_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sporulation protein SpoIID</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">spoIID</name>
        <name evidence="3" type="ordered locus">STH1164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1164"/>
    <dbReference type="EnsemblBacteria" id="BAD40149">
        <property type="protein sequence ID" value="BAD40149"/>
        <property type="gene ID" value="STH1164"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1164"/>
    <dbReference type="eggNOG" id="COG2385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021203_3_1_9"/>
    <dbReference type="OMA" id="HTENNED"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013486">
        <property type="entry name" value="SpoIID/LytB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013693">
        <property type="entry name" value="SpoIID/LytB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30032:SF4">
        <property type="entry name" value="PTHR30032:SF4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08486">
        <property type="entry name" value="SpoIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02669">
        <property type="entry name" value="SpoIID_LytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SpoIID" evidence="2">
        <location>
            <begin position="151"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="569" mass="58905" checksum="09A1B1598B8FEDEC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE8</accession>
    <name>Q67QE8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1110"/>
    <dbReference type="EnsemblBacteria" id="BAD40095">
        <property type="protein sequence ID" value="BAD40095"/>
        <property type="gene ID" value="STH1110"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1110"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OMA" id="PHGRVKA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="5"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="327" mass="35730" checksum="39BC926C9F964260" modified="2004-10-11" version="1">MEKLLVIENLSVEFPSPRGPLRAVDGVTLEVEAGEIVGLIGESGSGKSTVLLAVMGLIARPGRILPGSSVRLKGRELTRLTPKEYRAVRGYEIGMVFQDPMTALNPALPIGEQVRESLRVHRYCRSRAEEHARVIELLEAVGIVGAAQRYRSYPHQFSGGMLQRVLIASALACNPRLLLADEPTTALDVTIQAQILDLLKKINRERHTAILLVSHDIGLAAEFCDRIAVMYAGRIVEVGPAEQVVNAPRHPYTRGLVGCLPSWGGRRGRLEPIPGSLPDLTQLPRGCAFADRCAFALPQCREEEIPLRAMPDGRRVSCIRAEEAMVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QF2</accession>
    <name>SYFB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">STH1106</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195238.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QF2"/>
    <dbReference type="SMR" id="Q67QF2"/>
    <dbReference type="STRING" id="292459.STH1106"/>
    <dbReference type="EnsemblBacteria" id="BAD40091">
        <property type="protein sequence ID" value="BAD40091"/>
        <property type="gene ID" value="STH1106"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1106"/>
    <dbReference type="eggNOG" id="COG0072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016891_0_0_9"/>
    <dbReference type="OMA" id="ISYNWLK"/>
    <dbReference type="OrthoDB" id="53568at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000232822" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-binding" evidence="1">
        <location>
            <begin position="39"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="406"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="1">
        <location>
            <begin position="799"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Insert">
        <location>
            <begin position="442"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="547"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <sequence length="892" mass="96117" checksum="9F385BE2D6FF91EC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL3</accession>
    <name>Q67QL3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NifU-like nitrogen fixation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1045"/>
    <dbReference type="EnsemblBacteria" id="BAD40030">
        <property type="protein sequence ID" value="BAD40030"/>
        <property type="gene ID" value="STH1045"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1045"/>
    <dbReference type="eggNOG" id="COG0694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060555_4_3_9"/>
    <dbReference type="OMA" id="CSVNQMT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU" evidence="1">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8429" checksum="8B949CA8E7DF4E37" modified="2004-10-11" version="1">MAEETLFERVERALDLIRPAIRMDGGEVELVAVEDGVARIRMVGACGGCPMSTMTLKMGIERAVRQQVPEIRAVEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QN3</accession>
    <name>Q67QN3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-coenzyme A synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195157.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1025"/>
    <dbReference type="EnsemblBacteria" id="BAD40010">
        <property type="protein sequence ID" value="BAD40010"/>
        <property type="gene ID" value="STH1025"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1025"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_9"/>
    <dbReference type="OMA" id="AIKASWP"/>
    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACAS_N" evidence="3">
        <location>
            <begin position="31"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="90"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="532"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="649" mass="70912" checksum="93C615FBBF420C65" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU7</accession>
    <name>Q67QU7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH961</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH961"/>
    <dbReference type="EnsemblBacteria" id="BAD39946">
        <property type="protein sequence ID" value="BAD39946"/>
        <property type="gene ID" value="STH961"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH961"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_15_12_9"/>
    <dbReference type="OMA" id="VYVYFET"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039538">
        <property type="entry name" value="BetI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13977">
        <property type="entry name" value="TetR_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="22736" checksum="DAA40A615893D47D" modified="2004-10-11" version="1">MPRVSPAHMERRREAILNAAMEVFIAKGYQVATVGDIAQQAGLSVGAIYRYFPTKADLMLALVRERLGRAPALFARLTARVEDPWERLVRCVELFTAALRVRHPGTGRLLLVTMAEAVQNGEVRRGLHDRFAGLADYVAGIIAEGVARGRFRPDVDPRTVAALLLSMADGVAVYWVTGAPDLELNAMGQSLVAMLRSYLLPEPLLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R00</accession>
    <name>Q67R00_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195040.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH908"/>
    <dbReference type="EnsemblBacteria" id="BAD39893">
        <property type="protein sequence ID" value="BAD39893"/>
        <property type="gene ID" value="STH908"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH908"/>
    <dbReference type="HOGENOM" id="CLU_542814_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="502" mass="51652" checksum="68E2EB872558C1CD" modified="2004-10-11" version="1">MSDADKELRQLIKDEFDRSLAGWTFTPAMRQAVLDRIAGEGEPEATPVPTLSRRFRPAYWVAAAAAAFVLAINLWPRVSTDGRLTGTASDARSGGAPESAPGAEAAAYGLQATGELQALGAAPGQSGTADSAEADAASTLKAGGVRALSAISPVRLTLSVAEVPDVFVVERAGEKVSLHVSETTGRALTDGAEEAQAGATAAAVPDALDLNPLPDGNVVVVQRWAVKIVDQHGNPVAESPVEPEAAAVAEGPAGETVVVSSDALTLFTVTGKPAELIGLDQYPSLVAVSGTRMAVANDGGVEVYEGGARALTLPRLQPRAMALAPDGALAVLTGSPPDARLLIYAADGSLLLDRPVAPDGEGFGFIGDGLQVAVGSVVYDRTGDERWHFPFAPARVTALADGVSVLAWNSRQAARVLADGGTQVWMAEVTDADLVRASGSARGDLVVLVAAADDGAAVWVIDQGGNQRHAERLSRVPVDVTASGDRILLLNAEGLEVRPLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R03</accession>
    <name>Q67R03_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39890">
        <property type="protein sequence ID" value="BAD39890"/>
        <property type="gene ID" value="STH905"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH905"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_816180_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="351" mass="40903" checksum="4E9B08006F346181" modified="2004-10-11" version="1">MEALANARLHQKRPLDVRVPDLLWRDRGLTEGARYLWCFLWMTRTTCTKYTFAELRRATGLSQHSLLRHLEALSRKLWLQYTRSGRTVEVQPLWPRSCRYIVLTDDLLLDRSLPHAARWVWGVIRRLGRRFSYQKLIQLTGYCHNSLTKYLRVLQEKGHLTGTTCRVGRRKAFDLTASNPAESRRLAALAKFEKSKSLVQQWRAYSFGQFLLARMVEMLTGTVLLENGAASCLDNPETGARMQFDLFLPKYSVALEYQGPQHSRVTQRFPDAAELQRQQQRDRLKRQLSEAAGIRLIEVHPPDLSFRRLAELLREAGVPLRDLPDEERYVYQALLRHSQRYRAAVRQEAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R39</accession>
    <name>Q67R39_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH869"/>
    <dbReference type="EnsemblBacteria" id="BAD39854">
        <property type="protein sequence ID" value="BAD39854"/>
        <property type="gene ID" value="STH869"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH869"/>
    <dbReference type="eggNOG" id="COG2861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041643_2_1_9"/>
    <dbReference type="OMA" id="VMLHMPM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10936">
        <property type="entry name" value="CE4_DAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006837">
        <property type="entry name" value="Divergent_DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30105">
        <property type="entry name" value="PTHR30105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04748">
        <property type="entry name" value="Polysacc_deac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="27329" checksum="6E0D5D260EBA8342" modified="2004-10-11" version="1">MVAAFAPQDGYGSVFCNARPVPGQPPLLAIVIDDLTTGAPGLEEMLRLPYPLTFAILPDRPDAGALARRIAALGHEVILHLPMDAGEVDPQWYVGRPISSRQSDEEIQQLVSEWLAAVPEARGMNNHMGTVATQDERVVRAVLEVARRHGKYILDSMTTENTVVPRTAVEMGVPCMQRSLFLDHENGEEVVAAQLYKLAEWAETHGAAIGIGHVGVGREGTAAALAEVLPELEARGIRLVTLSQLLSYQQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R72</accession>
    <name>Q67R72_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH836</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194969.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH836"/>
    <dbReference type="EnsemblBacteria" id="BAD39821">
        <property type="protein sequence ID" value="BAD39821"/>
        <property type="gene ID" value="STH836"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH836"/>
    <dbReference type="eggNOG" id="COG0005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_1_0_9"/>
    <dbReference type="OMA" id="SCITDMA"/>
    <dbReference type="OrthoDB" id="1364220at2"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="PTHR11904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="4">
        <location>
            <begin position="25"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="273" mass="29255" checksum="9EF81A7A36DD42D4" modified="2004-10-11" version="1">MSDLLQRIEEARAAVAARGGVTPQVGLILGSGLGDLADQVEDAVKVPYNEIPHFPVSTAPGHAGRLVIGRLEGKPVVAMQGRVHFYEGYTMEQVTFPVRVMRALGVETLIVTCAAGGLNPSFSAGDLMLITDHINFMGQDPLRGPNDERLGPRFPATVGAYTPELRELAVAVAQEMGVSLRQGTYTAISGPCYNTSAELRMLQRLGGDAVGMSTVPEVVTARHMGLRVLGIACITDMALPDAPVHLTHEQVLEVANRTKPLFQNLVRGVVRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB0</accession>
    <name>Q67RB0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative long-chain fatty-acid-CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194931.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH798"/>
    <dbReference type="EnsemblBacteria" id="BAD39783">
        <property type="protein sequence ID" value="BAD39783"/>
        <property type="gene ID" value="STH798"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH798"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_9"/>
    <dbReference type="OMA" id="VHDHERI"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="37"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="419"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="501" mass="55882" checksum="12AB73AC27FC3080" modified="2004-10-11" version="1">MINTVGDWLGRRAIHTPFREALVVMPRAQRAGLGDPQAEPLRLTYDQWNRMANRMAHFLLEQGVRRGDRVAVLSQNCLEMLTLLFACGKLGAIYVPYNWRLTVAELRPLVADTDPRIFFYGPHFEQAVAELQPPKPVGLLNLDLSGYPEETPPAPDLDPEDPWMILYTGGTTGRSKGAVLSHRAVLWNAWNTIAGWTLSPDDRVPILTPFFHTGGLNVFTTPLVELGGCSILMGPFDPGELLDAVEKERLTVIFMVPTMFKMVMDHPRFAETDFSRVRFLISGGAACPEPVQLAYRAKGCQFKIGYGLTEAGPNTFGMPDHRSWDKPGSVGFPLPHIQLRIVAEDGRLCGTNEVGELLIKGGHVFSGYWRNPEATAEALQDGWLHTGDLARRDEEGFYYIVGRKKEMFISGGENVFPLEVEDAIYQHPAVAECAVVGIPDPKWGEVGKAFVVLKPGMRLTEEELIEHCRKLLAKYKIPKQVEFLPELPKNAAGKILKRELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB1</accession>
    <name>Q67RB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH797"/>
    <dbReference type="EnsemblBacteria" id="BAD39782">
        <property type="protein sequence ID" value="BAD39782"/>
        <property type="gene ID" value="STH797"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH797"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_908681_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="34855" checksum="C55C2A7935FB1A67" modified="2004-10-11" version="1">MGRSSEREEVRRMTTSLNPVSPAPRPSVWRQLRAGVTHPAGVLFLALWVAGAATLALRGRAELVLEVVFRLLIINLIPLVVLLRRTPYPPAPDAPRHRRWVLWVQVAFLAALIALGVLYVLGHYGLIPSRWTQVPVWTQANEALEHLGEKLFGQRRIVANPALNALLPGLVLWLGGVCPAELWLRRGHSSFAVAAPYLIFVAIAVVPPVIAGDTRTAAMVGLMLVRNFFVNGFTEEFLFRGALQTRLSQLLGEGWGLVLASLIFGVAHVGGLMPALDGDVLAALGLCITSRAAAGLIFGILAIRTRSLIAPTVAHLTLNLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ0</accession>
    <name>Q67RQ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH658</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH658"/>
    <dbReference type="EnsemblBacteria" id="BAD39643">
        <property type="protein sequence ID" value="BAD39643"/>
        <property type="gene ID" value="STH658"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH658"/>
    <dbReference type="eggNOG" id="COG0656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_9"/>
    <dbReference type="OMA" id="QGTWYLG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="18"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="31398" checksum="65F97641D14A26C6" modified="2004-10-11" version="1">MQPIPTLSLPSGERVPVLGQGTWTMGESRRSRREEAEALRLGLDLGMTLIDTAEMYADGGAEEVVAEAIAGRRDEVFLVSKVLPGNASRRGTIEACERSLRRLRTDRIDLYLLHWRGRHPLAETLEAFAELVRAGKIRYWGVSNFDTDDMEELTGLPGGAAVATNQVLYNLSRRGIEYDLLPWCRERRIPIMAYSPIEQGRILENTVLRRVAARHGATPAQVALAWLLRQKDVMVIPKAGRPEHVRENRAALDLELTAEDLAELDRAFPSPTRKVPLEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU3</accession>
    <name>Q67RU3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3825 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194748.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH615"/>
    <dbReference type="EnsemblBacteria" id="BAD39600">
        <property type="protein sequence ID" value="BAD39600"/>
        <property type="gene ID" value="STH615"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH615"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032124_0_0_9"/>
    <dbReference type="OMA" id="RIMYLAP"/>
    <dbReference type="OrthoDB" id="1668151at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024437">
        <property type="entry name" value="DUF3825"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12873">
        <property type="entry name" value="DUF3825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3825" evidence="1">
        <location>
            <begin position="132"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="367" mass="42952" checksum="4DBE9A44E7804B95" modified="2004-10-11" version="1">MRLEEFTYLPLDVKEKALRYLANMMGEPERPLDQLTADLERALDRAVRGGLFSYLREEQPVEDRARADAVLFPTGHTTPTGEEILAKCILNKNPNRQPWFGLFFQAARREGFVIGDLYFRTWNEGARFLEELASMAIPERWNYSQYQSKQQHPILKSYVEKTYERLKQQGRVLRNESKLLFNTGLLNVYFKEIYVLGEADPEYPQRVINARPVLENDRAVLELFLNQKPPMATYFDRITDVIFDPDLEINTDDIHIIDDNFDRIPPKYRNRKKSEIFALFQAAIEFARIMARRNYKLVVPQYYMGQIQFLMPIYLSGEFSGPPDFALVLQKMGDVYRGNTILTLDMAYQNARLIAKPDTTWLSPDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ5</accession>
    <name>Q67RZ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GntR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194697.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH563"/>
    <dbReference type="EnsemblBacteria" id="BAD39548">
        <property type="protein sequence ID" value="BAD39548"/>
        <property type="gene ID" value="STH563"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH563"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_2_1_9"/>
    <dbReference type="OMA" id="SRYSFEF"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="242" mass="28151" checksum="065C8C6E3059574C" modified="2004-10-11" version="1">MLDKQSPTPLYNQLVDLLIRTIQTSMKPDDPLPSEREICEKYGVSRTTVRAALSELEDLGYIYRRHGKGTYVSALWKEMKNLSEFYSFTEHMRQLGMKPETRILSFETGVANRPTAERMKIGEGSAIYRLRRLRLADGLPMMLELTFIPVHLFPGLTLEMLQSMPLYDLYSRRFNQEIRYADEEFYASLVQDKEAELLGVPVGSPCLRLVRTTCNLDNRVIEFTLSAARADQFVYKIRHRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S42</accession>
    <name>Q67S42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH516"/>
    <dbReference type="EnsemblBacteria" id="BAD39501">
        <property type="protein sequence ID" value="BAD39501"/>
        <property type="gene ID" value="STH516"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH516"/>
    <dbReference type="eggNOG" id="COG1149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077329_0_1_9"/>
    <dbReference type="OMA" id="LIDHETC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="60"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="203" mass="21407" checksum="A03DB7C15060C618" modified="2004-10-11" version="1">MPSRRGGWAVSAGKQTDRRGFFREGFRSLLRAFAETAQAAADEAAVRGAGGVRWLRPPGALPEAAFLLTCTRCGDCARACPAGAIRLLPESAGAAVGTPFIDPLMQPCDLCGRCMGACGPGALVPVAEPRQVRMGVAVIDPARCWAVQGSICDLCWQRCPFPDEAIRMVDGKPQVQPEQCTGCGQCAYVCVSTPPAITIQPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S69</accession>
    <name>Q67S69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH489</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194623.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH489"/>
    <dbReference type="EnsemblBacteria" id="BAD39474">
        <property type="protein sequence ID" value="BAD39474"/>
        <property type="gene ID" value="STH489"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH489"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_0_9"/>
    <dbReference type="OMA" id="FFQNAWY"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="23"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="229" mass="25119" checksum="6E28A0C8A4381BB8" modified="2004-10-11" version="1">MDRFAAFKWQALFRDWHILVEGLGRTASTAGLALLLALALGVVFGVLATAPWRSGRAVSRLYVSLIQNTPLVTQVFFLYYLLPHFDIMLPTPAVGVLGLGVYHGAYMTEVVRAGIQAIHRGQLEAALSQGFTYVQAMRHIILPQATQIVLPPMTNQAVALIKNSSILAMISGGDLMFQADSWSASTGYYGPAYLTVWAIYFAICFPLAQLARRWEERVRLSRGGEVSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S86</accession>
    <name>Q67S86_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH472"/>
    <dbReference type="EnsemblBacteria" id="BAD39457">
        <property type="protein sequence ID" value="BAD39457"/>
        <property type="gene ID" value="STH472"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH472"/>
    <dbReference type="HOGENOM" id="CLU_2902614_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="62" mass="7025" checksum="53A8DF641A06D837" modified="2004-10-11" version="1">MTPETHLLLCLLLMAVAVIAAMVIMDLPAPAQPPRRRRAKRRACRRGRAHLRVISPRKAMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA1</accession>
    <name>Q67SA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative branched-chain amino acid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH457</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194591.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39442">
        <property type="protein sequence ID" value="BAD39442"/>
        <property type="gene ID" value="STH457"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH457"/>
    <dbReference type="eggNOG" id="COG1296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065777_2_0_9"/>
    <dbReference type="OMA" id="FIVLFME"/>
    <dbReference type="OrthoDB" id="1866750at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="23610" checksum="59AA119921ECEAC9" modified="2004-10-11" version="1">MATTQTLPSLPPATFTAGSRAALPIVLGYLPIGFAFGVLAQTAGFSVAEVGLMSLLVYAGSAQFIGAEMFAAGAAAPAIISTTFLVNLRHLLLSTALLPSFRRNHPAVNALLAYGITDETFAVNASVLRGRPIGPGFVAGLHITSQASWIAASVAGALAGGIATNQEALGLDFALSAMFIGLLVPNLRGEEAGSRRVAALVAAALAVAMGLLGQPSWGVIVATVVAATVGVMLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-10-13" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC6</accession>
    <name>OBG_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Obg</fullName>
            <ecNumber evidence="1">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">obg</name>
        <name type="ordered locus">STH432</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194566.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SC6"/>
    <dbReference type="SMR" id="Q67SC6"/>
    <dbReference type="STRING" id="292459.STH432"/>
    <dbReference type="EnsemblBacteria" id="BAD39417">
        <property type="protein sequence ID" value="BAD39417"/>
        <property type="gene ID" value="STH432"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH432"/>
    <dbReference type="eggNOG" id="COG0536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011747_2_1_9"/>
    <dbReference type="OMA" id="VVFDWEP"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="SSF102741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000386330" description="GTPase Obg">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Obg" evidence="3">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="159"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="2">
        <location>
            <begin position="345"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="165"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="282"/>
            <end position="285"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="311"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01231"/>
        </source>
    </evidence>
    <sequence length="425" mass="46772" checksum="1454D41AD8A72913" modified="2004-10-11" version="1">MFVDVARIYVKGGDGGRGSNSVRREKYVPQGGPWGGDGGRGGDVVFVVDPGLNTLVDFKYQKHFKAERGEHGGPKGMHGRKGEDLVIKVPPGTVVKDDDTGEVLFDLVEPGQRAVVARGGRGGRGNMRFATPTNKCPTFYEKGEPGEERWLLLELKVVADVGLVGFPNAGKSTFLSAVSAARPKIANYPFTTLTPVLGVVDLGEGRSFVIADIPGLIEGAHQGVGLGHEFLRHVERTKVLIHVLDGAGTEGRDPLSDFDVIHNELRAYNPELAARPTLVAFNKMDLPDARENLPRVREALEKRGYRVFPISGATREGFRPLLEAAYDLIRQWVPPEPAAPEAEMVYRPKEEGWRIYKYGGVWHVEGKEIERLVAMTMWENDEAVARFLRILRLKGVEQALREAGAEDGDTVRVCDIEFELMADPV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD7</accession>
    <name>SPEH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="1">AdoMetDC</shortName>
            <shortName evidence="1">SAMDC</shortName>
            <ecNumber evidence="1">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">speH</name>
        <name type="ordered locus">STH421</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + S-adenosyl-L-methionine = CO2 + S-adenosyl 3-(methylsulfanyl)propylamine</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194555.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SD7"/>
    <dbReference type="SMR" id="Q67SD7"/>
    <dbReference type="STRING" id="292459.STH421"/>
    <dbReference type="EnsemblBacteria" id="BAD39406">
        <property type="protein sequence ID" value="BAD39406"/>
        <property type="gene ID" value="STH421"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH421"/>
    <dbReference type="eggNOG" id="COG1586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_3_9"/>
    <dbReference type="OMA" id="VYTCGEH"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006557">
        <property type="term" value="P:S-adenosylmethioninamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042286">
        <property type="entry name" value="AdoMetDC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042284">
        <property type="entry name" value="AdoMetDC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="PTHR33866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="SSF56276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_1000013683" description="S-adenosylmethionine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000315032" description="S-adenosylmethionine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="65"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="1">
        <location>
            <begin position="64"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <sequence length="132" mass="14274" checksum="430EB0E144D8DA4F" modified="2004-10-11" version="1" precursor="true">MAYSTYGRHITMDLREVAFEKLNDVRFLKEVMYEAAAQCGATVVGESFVQFSPQGVTGVLVLSESHLSIHTYPEEGFAAIDCYTCGYTVDPAVACDYLKAALGGRVVGYRALRRGSGAIEDLTTLHLAAANN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ2</accession>
    <name>SYV_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">STH366</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194500.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SJ2"/>
    <dbReference type="SMR" id="Q67SJ2"/>
    <dbReference type="STRING" id="292459.STH366"/>
    <dbReference type="PRIDE" id="Q67SJ2"/>
    <dbReference type="EnsemblBacteria" id="BAD39351">
        <property type="protein sequence ID" value="BAD39351"/>
        <property type="gene ID" value="STH366"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH366"/>
    <dbReference type="eggNOG" id="COG0525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_9"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224584" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="845"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="533"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <sequence length="911" mass="103067" checksum="FB05FF7210EF0EB9" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM7</accession>
    <name>Q67SM7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39316">
        <property type="protein sequence ID" value="BAD39316"/>
        <property type="gene ID" value="STH331"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH331"/>
    <dbReference type="HOGENOM" id="CLU_2319084_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023162">
        <property type="entry name" value="Apc36109-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116922">
        <property type="entry name" value="SSF116922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="11046" checksum="8A4E03A85BC736D4" modified="2004-10-11" version="1">MTMRDQDRLAAVTAVVTRAVNQTDPMGLLSIGAPEDEYEPEIRELVAGLGDAWPDPTAVLRLCESVFSHFFGARYVNAKRMKRLAGRIVMGLRELERTP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T14</accession>
    <name>GLMM_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="1">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmM</name>
        <name type="ordered locus">STH194</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194329.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67T14"/>
    <dbReference type="SMR" id="Q67T14"/>
    <dbReference type="STRING" id="292459.STH194"/>
    <dbReference type="EnsemblBacteria" id="BAD39179">
        <property type="protein sequence ID" value="BAD39179"/>
        <property type="gene ID" value="STH194"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH194"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_0_9"/>
    <dbReference type="OMA" id="PHNPEPL"/>
    <dbReference type="OrthoDB" id="1265792at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147984" description="Phosphoglucosamine mutase">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <sequence length="447" mass="47198" checksum="B89FFC086FD70B8E" modified="2004-10-11" version="1">MPRMFGTDGVRGVANTEDLSPELAFALGRAAATLARERSGGRVVGVIGRDTRRSGPMLAAALSAGICSAGGDVVDLGVITTPGVAYVTTHLKADFGVMISASHNPAPDNGIKFFSGDGYKLPDEVEDQLEALVKARPDTMPRPTGAELGSIRQSPEAVEAYVEHLVSTGSPLSGLRVVVDCGHGAAYRLSPEVLRRLGAEVIALNTAPDGLNINAGCGSTHPEALQEAVRAHGADAGIAHDGDADRCIAVDERGELVDGDQIMAICALDLKARGQLPNDTLVTTVMSNMGLEMLMRRHGIRLVRTKVGDRYVLEEMLKGGHGLGGEQSGHVIFGALSTTGDGILTAVQLLSIVAREKQPLSTLAGRMQRLPQWLENVRVGRKEGWEQNQAIQAAIARAEAEMAGQGRVLVRASGTEPLIRVMLEGTDMAHIQRLAASIAEVIRTELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T28</accession>
    <name>Q67T28_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH180"/>
    <dbReference type="EnsemblBacteria" id="BAD39165">
        <property type="protein sequence ID" value="BAD39165"/>
        <property type="gene ID" value="STH180"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH180"/>
    <dbReference type="eggNOG" id="COG1266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_799077_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="347" mass="37089" checksum="82DB3645FA346D15" modified="2004-10-11" version="1">MTTRQQVALLRVLAFYLTALVLLLVAGSALQYTLGLAGVVVSQLLIFVALPLVFTLAVEKRPVRPFLRLRMLSLRGVGRAVVLGLIGWLAAQLMGAVLVLLVQQMGGQMVQTYQILLDAGSTLALLVGALVPAFCEELSFRGYVLGALRPLGPGAAVVLTGLLFGALHLSLVRLIPLTLLGMLWALAVQRSGSILPGMIMHLINNGTALLLTFFVQDRATPAELQGLEMLPDTAVWGSMMMLTLAAAGLSALAYSLAAGFSPRHLARPVEAEVEWDWQAEGRTEPVPAPEALAAADGDVELQRLAAELAVLRRRRRRMLSAASLLTGGLTLSIYLWAVLQELATVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T50</accession>
    <name>Q67T50_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194293.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH158"/>
    <dbReference type="EnsemblBacteria" id="BAD39143">
        <property type="protein sequence ID" value="BAD39143"/>
        <property type="gene ID" value="STH158"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH158"/>
    <dbReference type="eggNOG" id="COG4732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100509_1_0_9"/>
    <dbReference type="OMA" id="PSFIMAT"/>
    <dbReference type="OrthoDB" id="1870679at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012652">
        <property type="entry name" value="ThiW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09512">
        <property type="entry name" value="ThiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024534">
        <property type="entry name" value="ThiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02359">
        <property type="entry name" value="thiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="181" mass="17996" checksum="CA87721C48ABEDDD" modified="2004-10-11" version="1">MNRAGLLTRMAILMALGVASSSFSAIPLFGAKLFPAQHAINVVAGAMLGPVYAGVVGLGIATIRILLGSGTLLAIPGTVFGAVLAGLLYRRTGSRLAAMIGEVIGTGLIGAVAAYPVAVLLLGNAKAAAGGIGFYIPSFAASSAAGALIGGQALAVLQRFRLVVTPRALQVPASGCDRPDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T62</accession>
    <name>Q67T62_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH146</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH146"/>
    <dbReference type="EnsemblBacteria" id="BAD39131">
        <property type="protein sequence ID" value="BAD39131"/>
        <property type="gene ID" value="STH146"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH146"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_9"/>
    <dbReference type="OMA" id="LMETAPW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="96"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="299" mass="32629" checksum="8E53C21BE02C1409" modified="2004-10-11" version="1">MAVAAEKKTEAHYGQAPKETTQLQQIVRRFMRNKAAVVGLVVLLLMYLVAIFAPYLASESLDAMNIPARFQPPSLEHPFGTDNLGRDVLSRMIWGSRVSLSVGFVAMTMAVAIGSLVGAVAGFYGGTWIDIVLMRIAEAIDIIPVFFLLITVVSVIRPSIFNIMLVIGVTSWPGLAQIVRSQFLSLRQRDFTEASRAMGARDARLIFRHILPNAVGPIIVSATLRIGSAILTESSLSFLGLGTQPPLTSWGQMLSQGRPFLLQAPWVSIFPGLAIFVVVICFNFVGDGLRDALDPKMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA1</accession>
    <name>Q67TA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH107</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194242.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH107"/>
    <dbReference type="EnsemblBacteria" id="BAD39092">
        <property type="protein sequence ID" value="BAD39092"/>
        <property type="gene ID" value="STH107"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH107"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050656_0_1_9"/>
    <dbReference type="OMA" id="THFCTMG"/>
    <dbReference type="OrthoDB" id="1899767at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="23"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="315" mass="33614" checksum="429314565C95303E" modified="2004-10-11" version="1">MTTQHILGWLLIAGVLSGFVFQRSRLCFVSALRDLFLFRALRMTRAMLLLFLLTVTGGATLQAVRGVLVEGFPGPTPAGALAGGAIFAVGMVLAGSCIAGAFWRLGEGQLSQLAVLAGVLAGTWLYVNLPFLTTVTVQETFNAWIAVGLLLLGLMAVAAWERRQPRVGEELPGARRLSWVRAPWAPELGAVVMAVLLVTFMALTGTGWGVSRAFLLTDLSAAAYAGGLLIGGFLGARLGREFRVRGPGGRAPTVIRLLGGVLMGYGARVGWGCTIGAVMTGMVNLSLHPWYWMLGAMLGAWVGAELLRRFMYQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA4</accession>
    <name>Q67TA4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mreB</name>
        <name evidence="3" type="ordered locus">STH104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194239.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH104"/>
    <dbReference type="EnsemblBacteria" id="BAD39089">
        <property type="protein sequence ID" value="BAD39089"/>
        <property type="gene ID" value="STH104"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH104"/>
    <dbReference type="eggNOG" id="COG1077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_9"/>
    <dbReference type="OMA" id="AIVRYMR"/>
    <dbReference type="OrthoDB" id="410781at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="206"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="340" mass="36205" checksum="D056A70E61266660" modified="2004-10-11" version="1">MFGWATDVGVDLGTATVLVYVKGKGVVLCEPSVVAMHRDTRQVLAIGEEARRMLGRTPGNIVAIRPLREGVIADYDVTEAMLKAFLRRVSGPRLLARPRMVICIPANVTTVEKRAVMEAAIEAGARTVTMVEEPLAAALGAGLDITQPAGHMVIDIGGGTTDVAVLSLGGVVLSESIRIGGDKFDEAIVRYMKMKYNLLIGERTAEEVKITVGSAGCDSRNESIEVRGRDLMSGLPKTVTITSAEVREAVSELVTSLCDTIKAVLEATPPELSADIQERGIVMTGGGAMLHGLDQVIAQETGVPAYLADDPVSCVARGTGRVLEEFEVEDNHLVVLRKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC4</accession>
    <name>Q67TC4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH84</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194219.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH84"/>
    <dbReference type="EnsemblBacteria" id="BAD39069">
        <property type="protein sequence ID" value="BAD39069"/>
        <property type="gene ID" value="STH84"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH84"/>
    <dbReference type="HOGENOM" id="CLU_2358630_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="96" mass="10415" checksum="E787BF4E6F10E0A5" modified="2004-10-11" version="1">MAAKIVAGILFGCAWGWVCNLVLFRQMANNRAAGFDSLRGIGVVFFVRYLLDAAALVLFYLIVRSGYALMAAALSITVAVKASLLYVYARKGGKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE0</accession>
    <name>Q67TE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH68</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194203.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH68"/>
    <dbReference type="EnsemblBacteria" id="BAD39053">
        <property type="protein sequence ID" value="BAD39053"/>
        <property type="gene ID" value="STH68"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH68"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1102343_0_0_9"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="3">
        <location>
            <begin position="150"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="252" mass="26167" checksum="266685264628362E" modified="2004-10-11" version="1">MQRRGNWQRIDWKKPHSAAGRGAAGGVSRRVPGVAGLPPVVVGLLIMGLVLGAGYLGWRRAVTPAAPAGDLPPARGGFLVAPPAGPAAFFAGVDEPGEPGDRAARMARWRQAAEAVVAARGGAGTDLTGAFAWPVTAPISSPFGPRWGRHHNGIDLAADHGEPIRASRAGEVLLAGEVEGYGLTVVIGHDDGTRTLYAHASALLVEAGEWVEQGQPIARVGSTGNSTGPHLHFEIIVGDRPVDPLDYLPPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF1</accession>
    <name>Q67TF1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative middle wall protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH57</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH57"/>
    <dbReference type="EnsemblBacteria" id="BAD39042">
        <property type="protein sequence ID" value="BAD39042"/>
        <property type="gene ID" value="STH57"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH57"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262217_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269533" evidence="2">
        <location>
            <begin position="32"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="90"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1291" mass="134541" checksum="00D46ABF0769DC49" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF3</accession>
    <name>Q67TF3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH55</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194190.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH55"/>
    <dbReference type="EnsemblBacteria" id="BAD39040">
        <property type="protein sequence ID" value="BAD39040"/>
        <property type="gene ID" value="STH55"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH55"/>
    <dbReference type="eggNOG" id="COG3267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024125_1_0_9"/>
    <dbReference type="OMA" id="TNFQLDD"/>
    <dbReference type="OrthoDB" id="947558at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="278" mass="31836" checksum="FF8A2992FBFE8987" modified="2004-10-11" version="1">MLAEFFGFRVLPFSKEIPTTDLFPCAQHQELLARLQYCVRHRAFGLITGEVGSGKTTAIRTLYDLLDRSRNPFVYIADSKLTPTAFYRDVLTQLGVAAPYHFLSREARRLFEQTILDGYRLHGLQPVIVLDEAHLLPHAMLQEVRFLLNFHLDSACPMTFLLVGQSELRGMLRLRTFEAIAHRVQVRYHLGPLSEEESVAYIRHHLRRTGVDHDIFSEQALQKIAAESRGLPRVINTLCTNCLLDACSREQRFVDEANVDRVLFELQDLQTAKGSSPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG3</accession>
    <name>Q67TG3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transposase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH45</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH45"/>
    <dbReference type="EnsemblBacteria" id="BAD39030">
        <property type="protein sequence ID" value="BAD39030"/>
        <property type="gene ID" value="STH45"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH45"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_8_0_9"/>
    <dbReference type="OMA" id="RQKHTKL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="17"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="170" mass="19641" checksum="2E98194A34012BC1" modified="2004-10-11" version="1">MDGFDRNEWSNPSGIRILVGNSGTGKTYLATALGIAACRQGYRVRFWRVGQFVAELQEAQNEHRLSRLEKQWLRFDLVVLDELGYVSLSRPASELLFRFVAARYERGSLIITTNLEFSEWPQVFGDQKMTAALADRVTHKAHIIEMNGESYRFRETLRRQEALKHHPETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI0</accession>
    <name>Q67TI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH28</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH28"/>
    <dbReference type="EnsemblBacteria" id="BAD39013">
        <property type="protein sequence ID" value="BAD39013"/>
        <property type="gene ID" value="STH28"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH28"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_1_9"/>
    <dbReference type="OMA" id="HWRRIER"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="104"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="19725" checksum="9B2CE86BBBFC678E" modified="2004-10-11" version="1">MTMEERFFALYRPEQGKLYRTAWAILGNEADAADALQEATIRAYRAFGQLRGGDVAFSAWMRRILVNTCTQILRKRMRVIPVERPEDLTAHPVTEVDLPLDGDVWDAVRSLDERYRAVVVLRFLNDMQLEEIAAALDIPLGTVKSRLHAAMKQLRSRLNALEERKEGRGVDAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TJ2</accession>
    <name>SYE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">STH16</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194151.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TJ2"/>
    <dbReference type="SMR" id="Q67TJ2"/>
    <dbReference type="STRING" id="292459.STH16"/>
    <dbReference type="EnsemblBacteria" id="BAD39001">
        <property type="protein sequence ID" value="BAD39001"/>
        <property type="gene ID" value="STH16"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH16"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_9"/>
    <dbReference type="OMA" id="WDEGPFF"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020752">
        <property type="entry name" value="Glu-tRNA-synth_I_codon-bd_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000119673" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <sequence length="482" mass="55490" checksum="EEC40EB190A7FBFD" modified="2004-10-11" version="1">MTVRVRIAPSPTGPIHVGNVHTALFNWLFARHHGGKFILRFEDTDLERSRPEWEQVIFEDLKWLGIDWDEGPDIGGPYGPYRQTERLDLYRKYAQQLLESGHVYKCYCTKEEEDADRREAQAAGRPYQYKGRCRDLTPEQQAAFEAEGRKPVLRFRVPRGEVIRFNDLVRGPIEVPTDSIGDFIIMRANGMPLYNFAVVVDDVTMNITHVLRGEGHIPNTPVQILIYQALGFPVPEFGHLGHMTNPERGKLSKRKGEAAIRDYREQGYLPEAMLNFMSLLGWTPPGAESGREFLTKEELIREFDLSRVTKASSVFDRNKLNWMNGVYIRKKSLEEFAELALPFVVSAGLCTEEQARARWDWFKEVMAQVHERVETLAEIPQHVDIFLKDEIEMDEKAARKFLTEAVKPFFQRVSEGLRSVEWSVPAIEQLVRSIQEEMGLTPKESFQPIRVAITGRTASPGLFETIYLIGRERVLERMAPYC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-02" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J28</accession>
    <name>RL34_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmH</name>
        <name type="ordered locus">STH3341</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J28"/>
    <dbReference type="SMR" id="Q67J28"/>
    <dbReference type="STRING" id="292459.STH3341"/>
    <dbReference type="EnsemblBacteria" id="BAD42322">
        <property type="protein sequence ID" value="BAD42322"/>
        <property type="gene ID" value="STH3341"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3341"/>
    <dbReference type="eggNOG" id="COG0230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129938_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00391">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000271">
        <property type="entry name" value="Ribosomal_L34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020939">
        <property type="entry name" value="Ribosomal_L34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14503">
        <property type="entry name" value="PTHR14503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00468">
        <property type="entry name" value="Ribosomal_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01030">
        <property type="entry name" value="rpmH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00784">
        <property type="entry name" value="RIBOSOMAL_L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000187482" description="50S ribosomal protein L34">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="9"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="47" mass="5693" checksum="92D1FF131D7B2211" modified="2004-10-11" version="1">MFLLKRTFQPHNRSRKRTHGFLSRMSTRGGRRVLKARRLKGRKRLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J38</accession>
    <name>Q67J38_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42312">
        <property type="protein sequence ID" value="BAD42312"/>
        <property type="gene ID" value="STH3331"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3331"/>
    <dbReference type="HOGENOM" id="CLU_1234491_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24984" checksum="0D8A25E485A136F3" modified="2004-10-11" version="1">MATLRRTQALPRRDLDCCRRTKSHEQHSSPTRTPRSHGLSRPDRLRSVSRGVWTVLVFHAEHRAPEGSAISRRWPFVPVAYRLQCRSCPPLSPRVSCSTVFHVEHRVCPALTTAASRASVSTPCQRSTWNTGPSGQHKLTTANRALPFTRTPYTARVRRRHTPQAETASDPSPTAPRCSTWNTLSGCLPPLLPSHGDTNLPRPPAHLLAARERARHAVRSTSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J59</accession>
    <name>Q67J59_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IDEAL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197422.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3310"/>
    <dbReference type="EnsemblBacteria" id="BAD42291">
        <property type="protein sequence ID" value="BAD42291"/>
        <property type="gene ID" value="STH3310"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3310"/>
    <dbReference type="eggNOG" id="COG5582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1593717_0_0_9"/>
    <dbReference type="OMA" id="KRRECAW"/>
    <dbReference type="OrthoDB" id="1613080at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1530.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014957">
        <property type="entry name" value="IDEAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014963">
        <property type="entry name" value="UPF0302_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038091">
        <property type="entry name" value="UPF0302_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027393">
        <property type="entry name" value="Virus_scaffolding_prot_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08858">
        <property type="entry name" value="IDEAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08864">
        <property type="entry name" value="UPF0302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00914">
        <property type="entry name" value="IDEAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IDEAL" evidence="1">
        <location>
            <begin position="127"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="167" mass="19606" checksum="206858605322D308" modified="2004-10-11" version="1">MERPRGTKSKADQIKAFLEQYQPKLSEARGFLQFMLSKEEILRRTDLVSDLTGYDCAILVSAKGSGTWPFLFRRGEKVYTKTSQAVVELMKSLPERIGLCLSTAPPPWSEEREEFLRMLARWHDEVFHEDLERMTRRQELLRQIDGALDRRDRELFEQLSAELRRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J81</accession>
    <name>Q67J81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3288"/>
    <dbReference type="EnsemblBacteria" id="BAD42269">
        <property type="protein sequence ID" value="BAD42269"/>
        <property type="gene ID" value="STH3288"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3288"/>
    <dbReference type="eggNOG" id="ENOG50313VY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141470_0_0_9"/>
    <dbReference type="OMA" id="CGMEANH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025673">
        <property type="entry name" value="PCYCGC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13798">
        <property type="entry name" value="PCYCGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="151" mass="16308" checksum="EDDA483BBC238073" modified="2004-10-11" version="1">MAVLLLALSGVVAGCSGQTGNHSRDAAQEATLPEGFPEAPDWYHHAPKPIQETYLAAAHHHDELQHIPCYCGCGAFHASNSDCYFQRDETGAVVAFDRHAVGCQICLDITRQTVAGLESGRSLTRIRQEIDEAYQAMGLTPTPTPLPPPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J83</accession>
    <name>Q67J83_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197398.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3286"/>
    <dbReference type="EnsemblBacteria" id="BAD42267">
        <property type="protein sequence ID" value="BAD42267"/>
        <property type="gene ID" value="STH3286"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3286"/>
    <dbReference type="eggNOG" id="COG1664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1377507_0_0_9"/>
    <dbReference type="OrthoDB" id="1968497at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007607">
        <property type="entry name" value="BacA/B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04519">
        <property type="entry name" value="Bactofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="198" mass="20362" checksum="3AD2AE7BB1717855" modified="2004-10-11" version="1">MFFGWRWKQDALPYSVIDRGTEVQGPIRSRGVLEVRGHVRGAVIHEGRMVVAPGGVCSGPVRSDDLYLQGEIHGDVTVTGTLRIGNGGQLYGDADCRRLIIDPGGCFVGINRSELEGRAAGPAAAQAGGAPSAVPVSNGAVPSPPTGLGVRSRGRRAARPGGHCRDHGPADGAARPGAADHRLPRVHAHEAPVGAGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J95</accession>
    <name>Q67J95_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3274"/>
    <dbReference type="EnsemblBacteria" id="BAD42255">
        <property type="protein sequence ID" value="BAD42255"/>
        <property type="gene ID" value="STH3274"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3274"/>
    <dbReference type="eggNOG" id="COG4932">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_360524_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="SSF49452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="SSF49464"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269225" evidence="1">
        <location>
            <begin position="24"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="776" mass="81738" checksum="9D5DAAE30884F3C7" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JG8</accession>
    <name>Q67JG8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stage II sporulation protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spoIIE</name>
        <name evidence="4" type="ordered locus">STH3200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197313.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3200"/>
    <dbReference type="EnsemblBacteria" id="BAD42182">
        <property type="protein sequence ID" value="BAD42182"/>
        <property type="gene ID" value="STH3200"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3200"/>
    <dbReference type="eggNOG" id="COG2208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017349_2_0_9"/>
    <dbReference type="OMA" id="ERVCKNC"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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            <end position="142"/>
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        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
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        <location>
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            <end position="197"/>
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    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="519"/>
            <end position="728"/>
        </location>
    </feature>
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        <location>
            <begin position="734"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAD42182.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="769" mass="80760" checksum="B0D8F01FC7641980" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK5</accession>
    <name>Q67JK5_SYMTH</name>
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        <submittedName>
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        </submittedName>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="STRING" id="292459.STH3163"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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            <end position="38"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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            <end position="143"/>
        </location>
    </feature>
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        <location>
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        </location>
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            <end position="295"/>
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    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="689" mass="75512" checksum="9C6F6A52CE4B243C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JP2</accession>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
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                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    </dbReference>
    <dbReference type="STRING" id="292459.STH3126"/>
    <dbReference type="EnsemblBacteria" id="BAD42108">
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        <property type="gene ID" value="STH3126"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG1694">
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    </dbReference>
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    <dbReference type="OrthoDB" id="1970248at2"/>
    <dbReference type="Proteomes" id="UP000000417">
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="132" mass="15085" checksum="9BC1E8FDEBAC1ABF" modified="2004-10-11" version="1">MKDLLCDQFQETVSECLIRHRSVLDIMAKYQESNARVNRALTKSVTSCGCIEIHGTKQRYPQDATLEQLRDHAMEHITGKLCDHCREVIEAEIGNNLFYLAALCNVLDLNLYDVLLKEHKKLSALGLYNFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JR2</accession>
    <name>Q67JR2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH3106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
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            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
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            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
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                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD42088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="193" mass="21556" checksum="85FFE0500ACDDCD9" modified="2004-10-11" version="1">MPVVSPERFATGLTWSEYLAGATEHKERRQAVYASVTFTPEQQAALAALRPLGLKVLVLEEYWCGDAARTGPLLARFAEEAGMEARWFFRDQNLDIMDQYLENGTSRAIPCFIFMDRNCEYIVHWGSRPRAVKEAVRPLQPLPPQGDPARPAAFERFVAVLSEAYDRVMPHGVTDELLEVLNSALRKSASGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JS1</accession>
    <name>Q67JS1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3097</name>
    </gene>
    <organism evidence="3 4">
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        <lineage>
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            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    <dbReference type="EnsemblBacteria" id="BAD42079">
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        <property type="gene ID" value="STH3097"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3097"/>
    <dbReference type="eggNOG" id="COG4585">
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    </dbReference>
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    <dbReference type="OMA" id="WEWHADE"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HATPase_c" evidence="2">
        <location>
            <begin position="259"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="368" mass="39516" checksum="0D2070AB9CB48AA3" modified="2004-10-11" version="1">MDLQGVWPVQAEWVSRAVAALPAAGSEPELAAAVARAAAEALGWRAAGVRRAGRTEWLARFPEGAEVRGEVNLPLAGGLELAADGAGDAEGLALLGAVAGKLLEAAQERARLAQRAERAERRAREAVERYRSQTERLAALRGELSEAQQRQLLLSERNRIAQDLHDRAAQTAYLVALKLDWLLGDLPPADPLREELERLRGLAAEAAAQTREAIFALRAPELSEGGLHGGLRRLLREAERDGFTTRLTVAGRPRALPAEVEDALFKVGQEALTNARKHSGGSAVMVALRYEPAAVTLVVQDNGAGIKNRTDIEKPGRFGVRGMRERVARLGGRLELVDGDEGGLLVRASVPLKGVDTHAHPCPDRGRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JT4</accession>
    <name>RPOC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta'</fullName>
            <shortName evidence="1">RNAP subunit beta'</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta'</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoC</name>
        <name type="ordered locus">STH3084</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197199.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JT4"/>
    <dbReference type="SMR" id="Q67JT4"/>
    <dbReference type="STRING" id="292459.STH3084"/>
    <dbReference type="PRIDE" id="Q67JT4"/>
    <dbReference type="EnsemblBacteria" id="BAD42066">
        <property type="protein sequence ID" value="BAD42066"/>
        <property type="gene ID" value="STH3084"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3084"/>
    <dbReference type="eggNOG" id="COG0086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_3_0_9"/>
    <dbReference type="OMA" id="YRNIRVE"/>
    <dbReference type="OrthoDB" id="4421at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01322">
        <property type="entry name" value="RNApol_bact_RpoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012754">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02386">
        <property type="entry name" value="rpoC_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000225583" description="DNA-directed RNA polymerase subunit beta'">
        <location>
            <begin position="1"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="796"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="871"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="878"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="881"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01322"/>
        </source>
    </evidence>
    <sequence length="1179" mass="132941" checksum="3B52D62C4E5E7201" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU3</accession>
    <name>RL3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplC</name>
        <name type="ordered locus">STH3075</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197190.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU3"/>
    <dbReference type="SMR" id="Q67JU3"/>
    <dbReference type="STRING" id="292459.STH3075"/>
    <dbReference type="EnsemblBacteria" id="BAD42057">
        <property type="protein sequence ID" value="BAD42057"/>
        <property type="gene ID" value="STH3075"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3075"/>
    <dbReference type="eggNOG" id="COG0087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_4_1_9"/>
    <dbReference type="OMA" id="KRMAGRY"/>
    <dbReference type="OrthoDB" id="1270636at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000241420" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <sequence length="216" mass="23467" checksum="600CE995FCDE9C0B" modified="2004-10-11" version="1">MQKGILGKKLGMTQIFAPDGKIIPVTVVEAGPCVVVQKKTTATDGYNAVQLGFGEIRETLVNKPLKGHFEKHQVKATRYLREFRLDDVESLNVGDVIKADIFAEGELVDVTGISRGKGFAGGVKRWNFNRGPSSHGSKYHRRPGSLGQRRWARVPKGRKLPGRLGGERVTVLGLRVVKVDPEKNLILIKGAVPGAKGSLITIRDSVKAARAKAKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KD0</accession>
    <name>Q67KD0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sigma-54-dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2885</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2885"/>
    <dbReference type="EnsemblBacteria" id="BAD41868">
        <property type="protein sequence ID" value="BAD41868"/>
        <property type="gene ID" value="STH2885"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2885"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_7_9"/>
    <dbReference type="OMA" id="DMSPAIQ"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="7">
        <location>
            <begin position="31"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="328" mass="35988" checksum="1CFC2E6111F9C192" modified="2004-10-11" version="1">MHRLEGGGRMRSTIPQAKPSSHQGQDPFAQLVGRDPAWLSALERARRAARTSATVLITGETGTGKELVARALHAASDRARGPFIAINAGTLLPDLLASELFGYEPGMFTGGNPRGHTGLIQSADGGTLFLDEVSELPYEAQVALLRTLQEGEVVRVGGHRPIPVDVRIVAATNRDLREMVRQGRFRQDLFYRLSVIPVHLPPLRERPRDIIPLIVHGYHRLNRGVPRLPLSSWRRLLRYDWPGNVRELLNLVQLAVTLDEDPAALLPLPPLPLSQAEPGPAGEEAAIRAALDACGGNASAAARRLGMSRSTLYRKLELYGIRLKRQVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE2</accession>
    <name>Q67KE2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41856">
        <property type="protein sequence ID" value="BAD41856"/>
        <property type="gene ID" value="STH2873"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2873"/>
    <dbReference type="HOGENOM" id="CLU_1585641_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="168" mass="18413" checksum="6009F191027CE7F4" modified="2004-10-11" version="1">MYTLRYPYHLPYFGGGLVAALLAWGFLQMAVLLEPEVKPGVQFIVGAGFAVVSWAHISVVACLTIIPQVVRAGRLLDTFDPIWILGGLSPLVVPAAAVALRIAGRPLLPSLAAALFRFSVPLGFLSLIPRWLHLPKQFRWLFLFTGVMFLVLDGLGFDFRPKGRARSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE3</accession>
    <name>Q67KE3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin reductase</fullName>
            <ecNumber evidence="1">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196989.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2872"/>
    <dbReference type="EnsemblBacteria" id="BAD41855">
        <property type="protein sequence ID" value="BAD41855"/>
        <property type="gene ID" value="STH2872"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2872"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_9"/>
    <dbReference type="OMA" id="HEMENYP"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="6"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="410" mass="44359" checksum="82B1345F3BF01267" modified="2004-10-11" version="1">MAERWDLVIIGGGPAGLSAAVYGARARMSTLLIEKGRPGGQAATTEEVENYPGLGRTTGPELMKQFREHAEALGATIVRGEVGAVELQGPVKTIRTKKGEVYEARAVILCPGAEPRMLGVKGEGRLRGKGVSYCATCDADFFTDLDVVVVGNGDAAVEEAIYLTRFASSVTLICIHPEGIMDANKAAQERLFATPKIKVIWQTMVEEILGEDHVEKVALRHLGTGERTELPCDGVFVFVGTTPRTEFLRGTGIELDRKGYIIADPDTMETNIPGVFAAGDARRKWLRQIVTAAADGAIAACAAEKFLAEEEQVDREILSPEEPVLAVFWSPADSLSVHVLQQAERIVAGMGGQMRLARIDCYKSARTAARLGVFDVPAVRIFHKGIPLATLTEDFNHLEEWVVGHLPARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE4</accession>
    <name>Q67KE4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2871</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196988.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2871"/>
    <dbReference type="EnsemblBacteria" id="BAD41854">
        <property type="protein sequence ID" value="BAD41854"/>
        <property type="gene ID" value="STH2871"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2871"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_1_9"/>
    <dbReference type="OMA" id="IQVDVDK"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="2">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="12024" checksum="C2FD9D2C3EA8ED68" modified="2004-10-11" version="1">MLEVNKENFEQEVLQASGPVVVDWWSPKCDPCMELLPEVERLAETYGDRVKFCKVNVLENRRLAISQRVLGLPTFLFFRDGEKVAELAGPEACTAEAIEAELQRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP2</accession>
    <name>Q67KP2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit J</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nuoJ2</name>
        <name evidence="2" type="ordered locus">STH2771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 6 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2771"/>
    <dbReference type="EnsemblBacteria" id="BAD41756">
        <property type="protein sequence ID" value="BAD41756"/>
        <property type="gene ID" value="STH2771"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2771"/>
    <dbReference type="eggNOG" id="COG0839">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085957_2_2_9"/>
    <dbReference type="OMA" id="MLVNKRE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0874">Quinone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="176" mass="19056" checksum="C79701D2B545AE1E" modified="2004-10-11" version="1">MTTDLLMSGQFWTFIILAVVTLVSTYRMVTTPLITHAALFMALSFTAVAGIFLLMQAEFIAAIQVLVYAGAITTMVIFAIMLSGLRDIKRQQQERVKLWRSNPAVLVVGAVFAAFMLYLYSTANLPAGGGERQVVPVSAIGSQLFTTYVIPFEVASILLLVAMIGAIILTAKEAES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KQ0</accession>
    <name>NPD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent protein deacetylase</fullName>
            <ecNumber evidence="1">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobB</name>
        <name type="ordered locus">STH2762</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class U subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196881.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67KQ0"/>
    <dbReference type="SMR" id="Q67KQ0"/>
    <dbReference type="STRING" id="292459.STH2762"/>
    <dbReference type="EnsemblBacteria" id="BAD41747">
        <property type="protein sequence ID" value="BAD41747"/>
        <property type="gene ID" value="STH2762"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2762"/>
    <dbReference type="eggNOG" id="COG0846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_0_9"/>
    <dbReference type="OMA" id="SMQVYPA"/>
    <dbReference type="OrthoDB" id="1264438at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034979">
        <property type="term" value="F:NAD-dependent protein deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006476">
        <property type="term" value="P:protein deacetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01968">
        <property type="entry name" value="Sirtuin_ClassU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028628">
        <property type="entry name" value="Sirtuin_class_U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000110361" description="NAD-dependent protein deacetylase">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="192"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="218"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01968"/>
        </source>
    </evidence>
    <sequence length="251" mass="27338" checksum="B5679993F077C151" modified="2004-10-11" version="1">MLEQIQDFARALQASRYAVALTGAGASTESGLPDFRSNTGLWKDVDPVSLISMTALRRRPVDFYRFYRMRFSHLWGAQPNPVHKVLAALQREGLLKRLITQNVDGLHQAAGSPDVIELHGSLRECQCLRCGRRFPSRLIDVEVETEADIPRCPECGGVLKPGVVLFEEALPADAIEAAIEAAMKADLFLVVGSSLEVGPANQLPVLAVQHGGRLAIFNLTPTFLDPRATWIFREKAGQALGALAAELGISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KV4</accession>
    <name>Q67KV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2707"/>
    <dbReference type="EnsemblBacteria" id="BAD41692">
        <property type="protein sequence ID" value="BAD41692"/>
        <property type="gene ID" value="STH2707"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2707"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_1_9"/>
    <dbReference type="OMA" id="WQGVEFL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="2"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="173"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="359" mass="40540" checksum="59DF890E8B8CCEFF" modified="2004-10-11" version="1">MEVLYCDLISQIAKAEVVIACPRPWVARFKSELGAKARVIELRDYNPASGLSWGRLRAVAQAIEDIKPDIVHTNEARMRVAVQLVRRLPPFRSGTFKHIHMQHIREVWRKSYLPERILTGSGPDLWIAVSHSVREFLINCRRVKPTSILFVPNYLALVHQYSPIESHDPGLRDSLGVSHDEVLIVAAGRLERQKGFDILIQAAHQLTSRCNAFRIVIAGEGSERERLQGLIEEYGLVERVRLLGWRNDLQELYSVADVFVLSSRWEGMPLTLIEALMSGPALVATKVDGVVDVMAGNHEVCGLIVPPDDPKALSDALFTVVADSKCRHELRKRVRQRATEMRHESNALPRLLDQAYQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KW3</accession>
    <name>Q67KW3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IS21 family transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196820.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2698"/>
    <dbReference type="EnsemblBacteria" id="BAD41683">
        <property type="protein sequence ID" value="BAD41683"/>
        <property type="gene ID" value="STH2698"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2698"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_1_1_9"/>
    <dbReference type="OMA" id="YLPMDRE"/>
    <dbReference type="OrthoDB" id="1792718at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="99"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="29957" checksum="11CFF5F730B30D5C" modified="2004-10-11" version="1">MSQELLLDYYLKRLKLPTVRRLYKELARDAAEHNKTFEEYLQALMEQEVIHREDNQLQRRLKAAGFPVPKTLESFDFTVMPSVNKAKILALSKSEYIRDRENILLVGNSGTGKTHLATALGIAACRQGYRVRFWRVGQFVAELQEAQNEHRLSRLEKQWLRFDLVVLDELGYVSLSRPASELLFHFVAARYERGSLIITTNLEFSEWPQVFGDQKMTAALADRVTHKAHIIEMNGESYRFRETLRRQEALKHHPETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KX7</accession>
    <name>Q67KX7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Acetolactate synthase</fullName>
            <ecNumber evidence="4 8">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH2684</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2684"/>
    <dbReference type="EnsemblBacteria" id="BAD41669">
        <property type="protein sequence ID" value="BAD41669"/>
        <property type="gene ID" value="STH2684"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2684"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_9"/>
    <dbReference type="OMA" id="FQEWPQV"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="11">
        <location>
            <begin position="3"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="9">
        <location>
            <begin position="192"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="10">
        <location>
            <begin position="394"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD41669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="571" mass="61492" checksum="07790DA1E096F98B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KZ7</accession>
    <name>Q67KZ7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196786.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2664"/>
    <dbReference type="EnsemblBacteria" id="BAD41649">
        <property type="protein sequence ID" value="BAD41649"/>
        <property type="gene ID" value="STH2664"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2664"/>
    <dbReference type="eggNOG" id="COG4586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="VEGPRQW"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="58"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="329" mass="37559" checksum="4A920D62FD968B41" modified="2004-10-11" version="1">MGAVIEVRGLRKEFKVARRKEGPFAALRTLFSREFDTRVAVDDVSFTIERGELVGYIGPNGAGKSTTIKMLTGILVPTAGEVRVRGVVPYLRRVENARQIGAVFGQRTQLWWDLPTVESFELLRHIYKVPRERYRQNMDLFRDLLGLDEFLNTPVRQLSLGQRMRADLAAALLHDPEIVYLDEPTIGLDVVAKERIRDFIRTINRERGVTVILTTHDMQDIEKICERMILIDHGRVIYDGPVAQIKERFGKHRTLVVDLEPNGPVVDVAVEGAELVRRDGHRIWLRFNRDELSASELIARVSARCAIRDLTVEEPEIEGIIARIYQEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L26</accession>
    <name>Q67L26_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2635"/>
    <dbReference type="EnsemblBacteria" id="BAD41620">
        <property type="protein sequence ID" value="BAD41620"/>
        <property type="gene ID" value="STH2635"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2635"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_9"/>
    <dbReference type="OMA" id="KHGAQGY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="7">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24186" checksum="3D57C5B4099AB1E2" modified="2004-10-11" version="1">MVDDHPLVRNGIRQVLSLEPDMEVVGEAGDDQEALRLAQELRPDVILLDLALPRRTGLELLPRLREELPETQVVILTYSASEQDAIAALQAGAAGYLVKSMAPEALAARIREAFRGDVPVSGSVMRNVLRAMSTPSELPPPTPGGQPGAGEAAESLLSPREAQILRRIAAGNTNREIAEQLGLSENTVKNYVKSILAKLNLENRVQAAAWALNNPWAVHEGDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L32</accession>
    <name>Q67L32_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycine betaine/carnitine/choline ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196751.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2629"/>
    <dbReference type="EnsemblBacteria" id="BAD41614">
        <property type="protein sequence ID" value="BAD41614"/>
        <property type="gene ID" value="STH2629"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2629"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_7_2_9"/>
    <dbReference type="OMA" id="NMGQATP"/>
    <dbReference type="OrthoDB" id="1233189at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="11"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="204" mass="21392" checksum="DFD05FFD0A3E1CC0" modified="2004-10-11" version="1">MITWAKLASLTLEHLRLSLSAVLLAVLVAVPAGILLSRIRRLADPVMVALGLFQTLPAIALLAFMIPLMGIGTKPALTALFLYALLPILSNTYKGLTGVDPAVKEAARGMGMTALQMLLSVELPLAFRVIMSGVRTSTVLIIGWATLGAYVGAGGLGEPIVSGFATVSPRLILAGGVPVTLMALLADFLLGRLERRVTPRGLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L61</accession>
    <name>Q67L61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Formate dehydrogenase beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196722.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2600"/>
    <dbReference type="EnsemblBacteria" id="BAD41585">
        <property type="protein sequence ID" value="BAD41585"/>
        <property type="gene ID" value="STH2600"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2600"/>
    <dbReference type="eggNOG" id="COG0437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_0_0_9"/>
    <dbReference type="OMA" id="PKYDYDN"/>
    <dbReference type="OrthoDB" id="1762646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="263" mass="28451" checksum="95748D60167292C5" modified="2004-10-11" version="1">MTYGMLIDLSKCMGCRGCQVACKQWNDLPGEDTSVGPDFTNPQKRSAYTWTTIDFVTAEVNGRTVATFTKSGCMHCLEPACESVCIAGAIKRQANGAVTIDHDKCIGCRYCQLGCPFGVPKYEYDKPVPRMQKCTLCYDRISEGLEPACAATCPSGAILFGEREELLKLARQRIAEGGGRYIDHVYGEHEAGGTAVLYISPVPLDLTALNTDVTTESVPGFTWKAMRQLPVLVGSVAALSLGLMAYSRRRAQLEALAKGGGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L81</accession>
    <name>Q67L81_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2580"/>
    <dbReference type="EnsemblBacteria" id="BAD41565">
        <property type="protein sequence ID" value="BAD41565"/>
        <property type="gene ID" value="STH2580"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2580"/>
    <dbReference type="eggNOG" id="COG4632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_844474_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269890" evidence="2">
        <location>
            <begin position="29"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="329" mass="35547" checksum="27B789A8A37FBC82" modified="2004-10-11" version="1">MRKRTRTLSATLALGLAASLSVGSVALAGTEVRVPVDGALDAYLAITSVVAAYPEEFYYEAEAPVTVTFHGDNLSWEDIEYLGEGYVEDGFVYLPDSGDTVRFAVKNYTYYEEAQVHDELTEEPMDEVFYVSGNYAVLTTPGYYSVVAAPLAVAPTRAIIQVRSSSAESGHTGQAASEPAAESPPESVVASPTTARVLVDGKEVAFEAYTIDGYNYFKLRDLAMALNVSEKQFQVTWDGEFNAINLVSGHPYTAVGGELTVCTDPEARQAVPTASKVFVNGRQVDLTAYNIGGYNYFKLRDVAKAMDFGVTWHQATSTIGIDTTSGYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LA4</accession>
    <name>Q67LA4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Threonine synthase</fullName>
            <ecNumber evidence="6 10">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">STH2557</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 10">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="6 10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196680.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2557"/>
    <dbReference type="EnsemblBacteria" id="BAD41542">
        <property type="protein sequence ID" value="BAD41542"/>
        <property type="gene ID" value="STH2557"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2557"/>
    <dbReference type="eggNOG" id="COG0498">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_0_0_9"/>
    <dbReference type="OMA" id="MWGFQAS"/>
    <dbReference type="OrthoDB" id="428821at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="11">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="BAD41542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="399" mass="41416" checksum="8A0F13D20CB89BD5" modified="2004-10-11" version="1">MARWHGVIRQYQRFLPVSERTPVVTLLEGNTPLVPAPRLGAELGLDLYLKFEGLNPTGSFKDRGMTLAMSKAVEAGAKAVICASTGNTSASAAAYAARAGLPCYVVIPDGAVAMGKLAQALMYGARTIQIEGNFDDGLRLVRRLTQEHPEIALVNSVNPYRLQGQKTAAFEVVDALGGYPDFLAIPVGNAGNITAYWMGFQEYAAAGVGAVESCSSSGAPAGALLPRMLGFQAAGAAPLVHGGEVEDPQTVGTAIRIGRPASRAGALAAARESGGAFWAVTDEEMLAAYRRLAATEGIFAEPASCASVAGLIKLCRLGYFEGRTGSAGDVSGVGGFIDGVPAPVPTHRPFTGRGGRPKVVAVLTGHGLKDPDTAIRVSPLPEKLPADYGRLERLILSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB7</accession>
    <name>Q67LB7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="1 5">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="1 5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2544"/>
    <dbReference type="EnsemblBacteria" id="BAD41529">
        <property type="protein sequence ID" value="BAD41529"/>
        <property type="gene ID" value="STH2544"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2544"/>
    <dbReference type="eggNOG" id="COG0538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_7_1_9"/>
    <dbReference type="OMA" id="CVRPCRY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004439">
        <property type="entry name" value="Isocitrate_DH_NADP_dimer_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43504">
        <property type="entry name" value="PTHR43504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00183">
        <property type="entry name" value="prok_nadp_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Iso_dh" evidence="6">
        <location>
            <begin position="29"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="421" mass="46540" checksum="F68B82DA31F355EF" modified="2004-10-11" version="1">MAKFTKLTPPEGQKITMENGRLVVPDNPIIPFIEGDGTGPDIWAAAQPVIDAAVEKAYGGRRKIAWFEVYAGEKCLNKFGEWLIDDTVEAIREYRVAIKGPLTTPVGGGIRSLNVTLRQVLDLYACVRPVRWFPGVPSPVKEPHKTDVVIFRENTEDIYAGIEWKAGTPEARKVQALLAEEMGVKFPNSDKSDMIGVGIKPVSEFGSKRLVRAAIQYAIDNKRKSVTLVHKGNIQKFTEGAFRDWGYQVALEEFRPYVITEEELWSDEYKGKMPEGKILIRDRIADITFQQLLLRPEEWDVIATLNLNGDYLSDHVAAIVGGIGIAPGANISYSGGYGLFEATHGTAPKYAGLDKVNPGSVILSGEMMLRYMGWHEAADLIIKGLSATIAQKTVTYDFARLTEGATEVKCSEFGRLIIANM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-09-13" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC1</accession>
    <name>PROB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gamma-glutamyl kinase</fullName>
            <shortName evidence="1">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proB</name>
        <name type="ordered locus">STH2540</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate = ADP + L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196663.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67LC1"/>
    <dbReference type="SMR" id="Q67LC1"/>
    <dbReference type="STRING" id="292459.STH2540"/>
    <dbReference type="EnsemblBacteria" id="BAD41525">
        <property type="protein sequence ID" value="BAD41525"/>
        <property type="gene ID" value="STH2540"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2540"/>
    <dbReference type="eggNOG" id="COG0263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025400_0_2_9"/>
    <dbReference type="OMA" id="INCYEGL"/>
    <dbReference type="OrthoDB" id="1480250at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000109741" description="Glutamate 5-kinase">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00456"/>
        </source>
    </evidence>
    <sequence length="262" mass="28006" checksum="F5F8F27132C6C5B6" modified="2004-10-11" version="1">MRERLRHCKRVIIKVGTSTLTHPGGHLHLGRMEALVRQIADLHFEGRQVILVTSGAVGAGLGRLGLAERPAEVAAKQALAAVGQGLLMQRYEGLFSEYGLVVGQVLLTREDLEDPDRRASSAQVMERLLAWGVIPIVNENDTVTSEEIRVGDNDTLSARVAALVRADLLILLSDVDGLYPADPHLHPGLSPIPWVSPDDDLDRFAGGPGSANGTGGMVTKVAAARICAEHGIPMVLACGERPDVLRQILAGEEIGTLFSREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD7</accession>
    <name>Q67LD7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2524"/>
    <dbReference type="EnsemblBacteria" id="BAD41509">
        <property type="protein sequence ID" value="BAD41509"/>
        <property type="gene ID" value="STH2524"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2524"/>
    <dbReference type="HOGENOM" id="CLU_756308_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="366" mass="40462" checksum="EF09BEB66915124B" modified="2004-10-11" version="1">MGMMAGQPIPFLRPALHALVRGPWRTLWIVSPSLHLAALQGLLPELERAGAEIRVLTDLSYQAIGDGRVELAALQRLRRLEGCEVRWQPDLGACIYASDEGGALVGSGPLTLDGLDGPRQYGVHLAEAGPVLSDLEQWWESARRLSPQEWAALEERVGLRRNALRIGAEITRLGAFVRVSAHGTRRTRRLDPRDFGVLALGNVRLRPMDVTLCRLDLVSRAKDELDAILAERGLEWNGQYLVPRHFLEQEWPGIFAAREKQLAEDLRSPEQQAAVAAQLAQARSAIAGFLGDLYPFVDADGRPADQWVAEQLERLVPEGLAAAVCAEAGVEYRVLTILPEDARSIAEMDELLRDPRLRSVQLTLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LI1</accession>
    <name>Q67LI1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2480"/>
    <dbReference type="EnsemblBacteria" id="BAD41465">
        <property type="protein sequence ID" value="BAD41465"/>
        <property type="gene ID" value="STH2480"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2480"/>
    <dbReference type="HOGENOM" id="CLU_3297565_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="40" mass="4399" checksum="93F7F177EA56E521" modified="2004-10-11" version="1">MAGTGNKWYQSPAVVGLIITLLGFGLFTLAFWLISIWLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LR7</accession>
    <name>Q67LR7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2394</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196517.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2394"/>
    <dbReference type="EnsemblBacteria" id="BAD41379">
        <property type="protein sequence ID" value="BAD41379"/>
        <property type="gene ID" value="STH2394"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2394"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_9"/>
    <dbReference type="OMA" id="QWYDSAK"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="145" mass="15173" checksum="97E97144C4002AB5" modified="2004-10-11" version="1">MTKVLLATDGSAHSVRAAQSLRKMAERDQTIEVTVFHVAPLPDLTTPAAAGAPVPNPLLPDTYAQAQAERALSVTLAALGLPPERARGSYVIGVPGEAILAEARQGGYDLIVMGRRGLSPLKELLLGSVSQVVLHRAPCPVLIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LS0</accession>
    <name>Q67LS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196514.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2391"/>
    <dbReference type="EnsemblBacteria" id="BAD41376">
        <property type="protein sequence ID" value="BAD41376"/>
        <property type="gene ID" value="STH2391"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2391"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_0_1_9"/>
    <dbReference type="OMA" id="GPIAMVP"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030489">
        <property type="entry name" value="TR_Rrf2-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01332">
        <property type="entry name" value="HTH_RRF2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="146" mass="15784" checksum="60CD69CE888372D2" modified="2004-10-11" version="1">MKLSTKGKYGVKALFELAMHEGSGPVSLRTIAERQGLSEHYLEQLAAPLRRAGIITAVRGAQGGYMLARPANQITVGDIIRVLEGPIGYTDCATEGEPDPECASTCPVHGVWERVTQQIIAVIDSITLQDLVDQALAERGNPMYHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT5</accession>
    <name>Q67LT5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2376"/>
    <dbReference type="EnsemblBacteria" id="BAD41361">
        <property type="protein sequence ID" value="BAD41361"/>
        <property type="gene ID" value="STH2376"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2376"/>
    <dbReference type="eggNOG" id="COG3764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1184550_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06166">
        <property type="entry name" value="Sortase_D_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042000">
        <property type="entry name" value="Sortase_D_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="24336" checksum="6E214591917CFFBD" modified="2004-10-11" version="1">MAGVVNGVSRREQARLMERYEREVAALAAAGGTAPGLAGLRPFAAAAPSGAPPALPEEGADTGAGLPAMETAGPEAGTGEESPDRADQAPGPEVIGILTIPKIDLTVAIAEGTDDRTLRHAVGHFAETAGPGEPGNFALVGHRGHIYGPFFLRLDQVEVGDPILVRSGGATYVYRASEIFVVEPDETWVLDPTEQAQITLITCTPKWINTHRLVVRGVLEAADLPEPVHTAGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX7</accession>
    <name>Q67LX7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2334"/>
    <dbReference type="EnsemblBacteria" id="BAD41319">
        <property type="protein sequence ID" value="BAD41319"/>
        <property type="gene ID" value="STH2334"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2334"/>
    <dbReference type="eggNOG" id="COG3883">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_690632_0_0_9"/>
    <dbReference type="OMA" id="NISKLPW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="399" mass="43202" checksum="AD1B18EED69B5FA9" modified="2004-10-11" version="1">MRSAVRPRGPDARRRGPHRAGEGSPAVVMTRERTARGGACMRGARLVGLLCLMALLLAVPAPRAQVAGAAGQDRAAVLQELFVLNRRLEEARAALADVEARLRAVSSQEEAALADLARLQEELAARREQYGRRLRYYREQGNRGPWVLLLTAGSLADFLWRLDALKQIMDYDARLARVLMETQAAVAAQAERLAQARAEADRLRDEQLARVAELEAAIADREAILAGLGDERAEVEEALAAVEADWQQSAMPVLDALGQALQQLGTAGLQPDDLRFSLFPPSAVATITEEQLNAYIGGYDALAGLRVDLVGEEEAFVLSGTFADVPVEISGGFLVLPDGRLRFEPSLMQVREFRVPEGVIAEIVSQGLLDIDVSSLVAPLTLTGVELTDGRMTVRAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M24</accession>
    <name>Q67M24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196409.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2286"/>
    <dbReference type="EnsemblBacteria" id="BAD41271">
        <property type="protein sequence ID" value="BAD41271"/>
        <property type="gene ID" value="STH2286"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2286"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_2_0_9"/>
    <dbReference type="OMA" id="WDHERTT"/>
    <dbReference type="OrthoDB" id="362657at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="124"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="501" mass="57521" checksum="C97CD29250CD9D92" modified="2004-10-11" version="1">MKIEMAHVERIRWLLHVEGKSQRQVAKALGISRKTVAKYAQLTKVPRYQRQKPAQPVVLTPEFQAEIERRLAENEILPRKQRWTGRTIYEDLVALGYQGSEPTVRRYIAKIRKLKRVQPAFIPLAHDPGESIEVDWGEAVVVLDGAEVTVQILCVRLRWSGMPVVIAYPTQRQEAMFDGLRRALERLSGVPRRMTLDNLKQAVKRILEGRNREEQDAFLSFRTHYLIDSNFCNPASGNEKGSVENLVGLAQRYIVGPHREARTWDELNAILWQRCLEYARRIPQGSTQSILERWEEEKKYLRPLPARPFDCCKVALVSSNKVSCVTFETCRYSVPVQYANRPLQVRAYWDRIDIYAGQEKIASHLRCYERNREILDLDHYLELLHVKPGALEQAKPYRMAQLPPVYHQFRAEMQAQGRSDREFIEILMLHRAYSVSEVSTALELALAQRTACYTAVKQLLAVQPDLGAEASTSTAGELAGIRVQQPSLAEYNRLLKGGVVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC7</accession>
    <name>Q67MC7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycerol-3-phosphate ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2181</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196306.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2181"/>
    <dbReference type="EnsemblBacteria" id="BAD41166">
        <property type="protein sequence ID" value="BAD41166"/>
        <property type="gene ID" value="STH2181"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2181"/>
    <dbReference type="eggNOG" id="COG1175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_0_2_9"/>
    <dbReference type="OMA" id="LWYSVQS"/>
    <dbReference type="OrthoDB" id="1688736at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="86"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="309" mass="34319" checksum="82BA66F0FCB2110C" modified="2004-10-11" version="1">MASQPAPATRARPRAPARPGFRRAERLEPYLYLTPSLALLTIFTFYPIVRSIYLSLFLTNPRGEPRVFVGLEHYVNALQSTFLQSLGVTVLFVLYTVPAGLLLGLVLALLAYQPLKGTRIFQLIFSSPLAISAAIGSTIWVMMLNPVSGMVNYLLSLVGIAPVHWLTDPRWALLAVGMVSIWLRLGFNFVLLLSGLQNVPEELHEAALVDGAGFWAKTWHITLPMLSPTLFFAAVVGVIHAFQVFAEIEIMTAGGPSDATNTVVYRIYQVAFRQYEFGAASAQAMLLFIVMVVLTYLQFRLGERRVHYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ME3</accession>
    <name>Q67ME3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196290.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41150">
        <property type="protein sequence ID" value="BAD41150"/>
        <property type="gene ID" value="STH2165"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2165"/>
    <dbReference type="HOGENOM" id="CLU_2921095_0_0_9"/>
    <dbReference type="OrthoDB" id="1823189at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="61" mass="6956" checksum="90CAFA2F1C9A9E0B" modified="2004-10-11" version="1">MGHGQPPRGRTGEKVTESGTYQCEDQTRWTYQAGERFRECPSTGKPTVWEKTSEPEHADSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MG1</accession>
    <name>Q67MG1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2147"/>
    <dbReference type="EnsemblBacteria" id="BAD41132">
        <property type="protein sequence ID" value="BAD41132"/>
        <property type="gene ID" value="STH2147"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2147"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3206144_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="45" mass="5509" checksum="5521DBDFCB93445D" modified="2004-10-11" version="1">MEMEPVQMRLKAPRERVFADWTEPDLLVRWWGPEARAEVDLLVRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK0</accession>
    <name>Q67MK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2108</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2108"/>
    <dbReference type="EnsemblBacteria" id="BAD41093">
        <property type="protein sequence ID" value="BAD41093"/>
        <property type="gene ID" value="STH2108"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2108"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="LADFCTH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025302">
        <property type="entry name" value="DUF4162"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13732">
        <property type="entry name" value="DUF4162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="296" mass="31801" checksum="175EB8BA6FF9A460" modified="2004-10-11" version="1">MRDVSFTVEPGAIYGFVGPNGAGKTTTMKIIATLLRPDAGSVFIGDTDALKRPGEARTRLGYMPDFFGVYDNLKVDEYMQFYADCYDVPTDGRDRLIGSLLGLVGLADKRGAYVDTLSRGMKQRLCLARSLVHDPDVLILDEPASGLDPRNRVEMREILLELSRRGKTILISSHILAELAEMCTHIGIIHAGRMVASGPVEQILREAGARVIEVRIAGAPADDGSARAAELARAVPGVRSVSVDDGALRVALDGDDQAAAELLARLVQAGLPVAHFAEVKSKLEDVFMAITGGEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT7</accession>
    <name>Q67MT7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-alanyl-D-alanine carboxypeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196147.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2021"/>
    <dbReference type="EnsemblBacteria" id="BAD41006">
        <property type="protein sequence ID" value="BAD41006"/>
        <property type="gene ID" value="STH2021"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2021"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020027_2_1_9"/>
    <dbReference type="OMA" id="HTGGIDG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023650">
        <property type="entry name" value="Beta-lactam_class-A_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00146">
        <property type="entry name" value="BETA_LACTAMASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="60"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="402" mass="42756" checksum="6C41ED6D5A5598DC" modified="2004-10-11" version="1">MRRWQRLALVALAGAVALGAGSIGYLRWHTRAVVTAAVPAGSHAVATTLDPAASDRLMQVVSDARERLGVPALQVAVVKGGELVWTGAVGWADPPAGRPVTAEDRFHIGSVSKLYTAALVLRLAEEGRLSLEDPVSRFVPDVPNGDRITVRQLLNHTSGLANYTEDTAFNLKTVLLRRRWSVDEVLDVIRQQAPRSAPGTEHYYSNSNYVLLGRIAEVAGGRPFADLLHDEVLRPLGLTNTYLAAGEPESVGTVRGYDVTVLGTGRMGIKLDMERFRAPFETSAFAAGAVTASAADVARFTSGLLGGRLLADSTLQSMLTFVDAPDEDMPAQTGYGLGVRRLVIGGEEMVGHTGTLPGYSNVALYAPAHDHAVAVLSNLSMSDVTDVLAEVQAVLLEPASTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MT8</accession>
    <name>Q67MT8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore coat protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Spore coat</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CotF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196146.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2020"/>
    <dbReference type="EnsemblBacteria" id="BAD41005">
        <property type="protein sequence ID" value="BAD41005"/>
        <property type="gene ID" value="STH2020"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2020"/>
    <dbReference type="eggNOG" id="COG5577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163858_1_0_9"/>
    <dbReference type="OrthoDB" id="1983281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012851">
        <property type="entry name" value="Spore_coat_CotF-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07875">
        <property type="entry name" value="Coat_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0167">Capsid protein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0946">Virion</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="10786" checksum="4E66AA0198C9014E" modified="2004-10-11" version="1">MSSVISAIGEAMGMKPDDRVIAEGLLTAAKLKATAYCAAVLETTNPDLRRLLTTHLTDALAEHERCTRLAIERGWYRAHASPEDLVSQSLQDARHVLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MU2</accession>
    <name>Q67MU2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH2016</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196142.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2016"/>
    <dbReference type="EnsemblBacteria" id="BAD41001">
        <property type="protein sequence ID" value="BAD41001"/>
        <property type="gene ID" value="STH2016"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2016"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_4_9"/>
    <dbReference type="OMA" id="CDEYRSI"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="16"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="332"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD41001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="575" mass="62846" checksum="F09304FF6BFA8D77" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MV6</accession>
    <name>Q67MV6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sigma70_r4_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196129.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2002"/>
    <dbReference type="EnsemblBacteria" id="BAD40987">
        <property type="protein sequence ID" value="BAD40987"/>
        <property type="gene ID" value="STH2002"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2002"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2276043_0_0_9"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r4_2" evidence="2">
        <location>
            <begin position="39"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="102" mass="11481" checksum="B4AFAA4EB53F2832" modified="2004-10-11" version="1">MSRFHGALREARKARKPPTAAPSLPCAPPDQAVEAREERLRLARALASLPEAYREALILREYEGLRYAEVGQVLGGSETWARVTCFRARRMLKEAYLRLEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX4</accession>
    <name>Q67MX4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anaerobic glycerol-3-phosphate dehydrogenase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196111.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1984"/>
    <dbReference type="EnsemblBacteria" id="BAD40969">
        <property type="protein sequence ID" value="BAD40969"/>
        <property type="gene ID" value="STH1984"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1984"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_7_1_9"/>
    <dbReference type="OMA" id="CGMPKLE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017753">
        <property type="entry name" value="G3P_DH_GlpC_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03379">
        <property type="entry name" value="glycerol3P_GlpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="44814" checksum="17B3FF13459F7710" modified="2004-10-11" version="1">MIHEYDACLKCTVCEMHCPVLRVAPEFPGPKNGGPGLARLRAAGEVVELDHLDLCLGCRTCDTVCPSGIRPAELVNAPKVAQVSRRGLPLREWVLTHTYLFGPLAVRMPGLVNGVLANGPIRWTMEKVLHIDRHKPMPKYANHSFREWFRRRRSPWQDRPAPNGRVVYFHGCSVQYMEPHIGRHVVEVLEHNGFEVVLPPQKCCGLPLIANGDLKSAAGNGRYNVQHLREWAAQGVPIVVSSTSCSLTLKHDYPGLLGLDGAGAVAEQVYDLFEFLMACHEQGLLRTDFRPIRERLPYHQPCHQRAQAIGLPAVELLNLIPGVEAWNLDAGCCGSAGTYGFKEEKYGVSMRVGQAMADALRASGASRAVSDCETCRWQIEYQAGMQAVHPVSVLWGAYGLGDRKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX7</accession>
    <name>Q67MX7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1981</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1981"/>
    <dbReference type="EnsemblBacteria" id="BAD40966">
        <property type="protein sequence ID" value="BAD40966"/>
        <property type="gene ID" value="STH1981"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1981"/>
    <dbReference type="eggNOG" id="COG0428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015114_1_2_9"/>
    <dbReference type="OMA" id="IAIPMRA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="271" mass="28174" checksum="04B2EA17A4FE096F" modified="2004-10-11" version="1">MVRCMREVGRPMDLAANANTILLGLLASLLAGLATVIGALPTLFTRSISPRIQDGLLGMAAGVMLAATIFSLLIPAMEYGGGGLRGALVAAVGLFVGGAFLDLIDRYSPHQHFIKGPEGGGATSSLQRIWLFIIAITIHNFPEGLAVGVGVGSGDLGSGLSLAMGIGLQNIPEGMAVALALLSERYALREVFLITLLTGLVEPVGGLLGVAAVTLMHPILPWALAFAGGAMLFVISDEIIPETHERGNERTATYMLLLGFVIMMLLDTTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N02</accession>
    <name>Q67N02_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1956</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1956"/>
    <dbReference type="EnsemblBacteria" id="BAD40941">
        <property type="protein sequence ID" value="BAD40941"/>
        <property type="gene ID" value="STH1956"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1956"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1626224_0_0_9"/>
    <dbReference type="OMA" id="YKHALAG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="13"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="163" mass="17232" checksum="B252ECAE45382556" modified="2004-10-11" version="1">MPVAWDEARPVASMVLPVACLALLPLVTVARITAQAMAGVYGQDYIRTARAKGLPERVVIYKHALAGALVPILDAMPGVLTVVFSNALVVELLFHYPGVTNLLQDAASPPGLVADLRRSLPPPDVPVLVAAGASLGLIFALLYAAVSVLRRVVDPRLRGRDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N69</accession>
    <name>Q67N69_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1889"/>
    <dbReference type="EnsemblBacteria" id="BAD40874">
        <property type="protein sequence ID" value="BAD40874"/>
        <property type="gene ID" value="STH1889"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1889"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027872_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="572"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="369"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="652" mass="74176" checksum="0CF153E13A65E65C" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N87</accession>
    <name>Q67N87_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195998.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40856">
        <property type="protein sequence ID" value="BAD40856"/>
        <property type="gene ID" value="STH1871"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1871"/>
    <dbReference type="eggNOG" id="ENOG5032SV6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070500_1_0_9"/>
    <dbReference type="OMA" id="CRSEVVQ"/>
    <dbReference type="OrthoDB" id="1695367at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043749">
        <property type="entry name" value="DUF5694"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18950">
        <property type="entry name" value="DUF5694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="27403" checksum="58777FB5A9F0FBE1" modified="2004-10-11" version="1">MVQERAQVMVLGTFHMENPNLDYVKTGYGDVLSEPYQQQIQEVVARLLRYRPTHVAVEAPPDAAPALQERYDQYRAGGYTLGRNEIDQLGFRTAAAMGHSRIFGIDYRLDLDFSAVLDEAGKLGLTGFLNAFERMTRSVAEAQERAEADGGVLGLLRYLNSPDHDAMHGVYLQIAAVGAGASYVGARLVADWYRRNLFMFSHIADLARQPEDGILVLVGAGHRPLLRQFITESPDLEYVDPLPYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N99</accession>
    <name>Q67N99_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stage III sporulation protein AD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spoIIIAD</name>
        <name evidence="2" type="ordered locus">STH1859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195987.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1859"/>
    <dbReference type="EnsemblBacteria" id="BAD40844">
        <property type="protein sequence ID" value="BAD40844"/>
        <property type="gene ID" value="STH1859"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1859"/>
    <dbReference type="eggNOG" id="ENOG5032SJW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159353_1_1_9"/>
    <dbReference type="OMA" id="GAQICRD"/>
    <dbReference type="OrthoDB" id="1696273at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025664">
        <property type="entry name" value="Spore_III_AC/AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014211">
        <property type="entry name" value="Spore_III_AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06686">
        <property type="entry name" value="SpoIIIAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02849">
        <property type="entry name" value="spore_III_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="128" mass="13541" checksum="D63F543264DA7ADE" modified="2004-10-11" version="1">MEIVQIVGLGLLAGMLISVLRQHRPELAMQLSIAAGVMLFALMMAKVLRVVEVIQSLAARASLEQAHIDTVLKIIGIAYITDFGAQVLSDAGEKAVATKVEMAGKIIIMLLAVPIILGVLDAILNLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NB0</accession>
    <name>Q67NB0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="2">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1848</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1848"/>
    <dbReference type="EnsemblBacteria" id="BAD40833">
        <property type="protein sequence ID" value="BAD40833"/>
        <property type="gene ID" value="STH1848"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1848"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_3_0_9"/>
    <dbReference type="OMA" id="SHQEGHL"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaD" evidence="7">
        <location>
            <begin position="40"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="320" mass="34127" checksum="AA00DEE264EDE565" modified="2004-10-11" version="1">MQTRLFLGIDTSYYTCSVALVDEDGRLLEDQRRLISVAEGERGVAPNEAVWAHLNALPDLAEPVLRRHGRSVAAVAASVKPRPVEGSYLPPFRVGEGFGRALASALGVPFYPTTHQEMHIQAGLWSADTPPRGDRFLAVHLSGGTTELIRVTRRPGGFGEELLGGTQDLHAGQFVDRVGVALGLPFPAGPHLERLAASGEPGRVRLPSAVRGYTVSFSGPESAAQRLVGQAAPADLALAVLHTVARTVEKWLLHAMEETGLREVLIVGGVAANQIVRARLRHRLEHPAVGARLAFAQPRFSTDNAVGTALLARACHGVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND6</accession>
    <name>Q67ND6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Alanine dehydrogenase</fullName>
            <ecNumber evidence="3 6">1.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH1822</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + L-alanine + NAD(+) = H(+) + NADH + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18405"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="1.4.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.1" evidence="3 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195950.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1822"/>
    <dbReference type="EnsemblBacteria" id="BAD40807">
        <property type="protein sequence ID" value="BAD40807"/>
        <property type="gene ID" value="STH1822"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1822"/>
    <dbReference type="eggNOG" id="COG0686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003376_3_0_9"/>
    <dbReference type="OMA" id="HFLTKQA"/>
    <dbReference type="OrthoDB" id="673280at2"/>
    <dbReference type="UniPathway" id="UPA00527">
        <property type="reaction ID" value="UER00585"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000286">
        <property type="term" value="F:alanine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05305">
        <property type="entry name" value="L-AlaDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008141">
        <property type="entry name" value="Ala_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008143">
        <property type="entry name" value="Ala_DH/PNT_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42795">
        <property type="entry name" value="PTHR42795"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000183">
        <property type="entry name" value="Alanine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00518">
        <property type="entry name" value="alaDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00837">
        <property type="entry name" value="ALADH_PNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AlaDh_PNT_N" evidence="11">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="AlaDh_PNT_C" evidence="10">
        <location>
            <begin position="149"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="239"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="267"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000183-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="372" mass="39777" checksum="43687C008069032B" modified="2004-10-11" version="1">MRVGVVRELKAYENRVAMTPAGVMAMVRAGHEVYVEHDAGRGSGFDDSEYVAAGARILPSNEDVWATADMIVKVKEPLPPEYDLMREGQIIFTYLHLAAEPELTRKLIERKSIAIAYETVQPADRSLPLLTPMSEVAGRMAVQIGAHYLEKAHGGRGVLLGGVPGVAPGRVAIIGGGTVGTNAAKIALGMGAHVTILDINADRLRYLDDIFGSRVETRISNEFEIANAVAQADLVIGAVLIPGAKAPKLVTAEMVKRMAPGSVIVDVAIDQGGSVETIDRVTTHQDPVYERFGVVHYAVANIPGAVPRTSTWALTNVTLPYALKLANLGWKEALRQDPALAKGANVVSGHVTYPAVAEAHGLEYVELDNVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NE4</accession>
    <name>Q67NE4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Anti-sigma F factor antagonist</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Stage II sporulation protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">spoIIAA</name>
        <name evidence="7" type="ordered locus">STH1814</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts SpoIIAB and thus releases sigma f from inhibition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the anti-sigma-factor antagonist family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195942.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1814"/>
    <dbReference type="EnsemblBacteria" id="BAD40799">
        <property type="protein sequence ID" value="BAD40799"/>
        <property type="gene ID" value="STH1814"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1814"/>
    <dbReference type="eggNOG" id="COG1366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115403_7_0_9"/>
    <dbReference type="OMA" id="ELDQYNA"/>
    <dbReference type="OrthoDB" id="1864829at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043856">
        <property type="term" value="F:anti-sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045152">
        <property type="term" value="F:antisigma factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003658">
        <property type="entry name" value="Anti-sigma_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014237">
        <property type="entry name" value="Anti-sigma_F_ant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00377">
        <property type="entry name" value="ant_ant_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02886">
        <property type="entry name" value="spore_II_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0749">Sporulation</keyword>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="123" mass="13540" checksum="36CBB1CEBDF975DD" modified="2004-10-11" version="1">MSSMEYELVGQSLIVRLSGELDLVAAARFKETVEDIMDRRPVRHLYVNLARVTFVDSAFLGALFGRYRRITREGGRVGVIGVPPQVRSALEFSGVFRTMEEFRSEQEALSADRAGSGAGEGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NL8</accession>
    <name>Q67NL8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195868.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1740"/>
    <dbReference type="EnsemblBacteria" id="BAD40725">
        <property type="protein sequence ID" value="BAD40725"/>
        <property type="gene ID" value="STH1740"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1740"/>
    <dbReference type="HOGENOM" id="CLU_2221884_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035238">
        <property type="entry name" value="DUF5345"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17280">
        <property type="entry name" value="DUF5345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="11603" checksum="D17866F6C1474D70" modified="2004-10-11" version="1">MKREPEPWERSLRDGLDAVAGAVDGEQPPDLGALIMLVDDVQRAQRLALRRDLRRFVAVAALILFGWLWAWLQFPAYFLVAQGLLAAALAAGAALAQAAGRRVSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN6</accession>
    <name>Q67NN6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyanophycinase</fullName>
            <ecNumber evidence="4">3.4.15.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1722</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) into aspartate-arginine dipeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[L-4-(L-arginin-2-N-yl)aspartate](n) + H2O = [L-4-(L-arginin-2-N-yl)aspartate](n-1) + L-4-(L-arginin-2-N-yl)aspartate</text>
            <dbReference type="Rhea" id="RHEA:12845"/>
            <dbReference type="Rhea" id="RHEA-COMP:13728"/>
            <dbReference type="Rhea" id="RHEA-COMP:13734"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:137986"/>
            <dbReference type="ChEBI" id="CHEBI:137991"/>
            <dbReference type="EC" id="3.4.15.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S51 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.15.6" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195850.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1722"/>
    <dbReference type="EnsemblBacteria" id="BAD40707">
        <property type="protein sequence ID" value="BAD40707"/>
        <property type="gene ID" value="STH1722"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1722"/>
    <dbReference type="eggNOG" id="COG4242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876962_0_0_9"/>
    <dbReference type="OrthoDB" id="1485646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005320">
        <property type="entry name" value="Peptidase_S51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011811">
        <property type="entry name" value="Peptidase_S51_cyanophycinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03575">
        <property type="entry name" value="Peptidase_S51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032067">
        <property type="entry name" value="Cyanophycinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="317" mass="33476" checksum="7E48DDE4CDA1CF5E" modified="2004-10-11" version="1">MNLERLGSITSELSTSLLKSIVVPQRSLRAWFARHRSSNPRNKNGRPDGRPERELDVSTAEFPAGPVILMGGTPVPDEAVVAAVHLSGGRSARLAVVPAAAGGDAAEAAAVAARAFTRFGMKHVEPVLLDSREKAADPEWVARLREYDAIVLCGDSPARGLHVLQATAAAAALRDHVKAGRLLVGIDAGAAILGSRLFPHEAEDGITVGLGIVPALLIDPGFSGAQRFSRLVRAMSAPEAAAMMGVGLDAQTGVLIADGEAKVLGEARVTVLDPWERGTPSEDRPGGLKVHLLTDGYRLDFRTRRAFPPERPEPNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS0</accession>
    <name>Q67NS0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C4-dicarboxylate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1688</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DcuC/DcuD transporter (TC 2.A.61) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195816.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1688"/>
    <dbReference type="EnsemblBacteria" id="BAD40673">
        <property type="protein sequence ID" value="BAD40673"/>
        <property type="gene ID" value="STH1688"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1688"/>
    <dbReference type="eggNOG" id="COG3069">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030262_1_0_9"/>
    <dbReference type="OMA" id="LSHAPYN"/>
    <dbReference type="OrthoDB" id="1345750at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015556">
        <property type="term" value="F:C4-dicarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004669">
        <property type="entry name" value="C4_dicarb_anaerob_car"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018385">
        <property type="entry name" value="C4_dicarb_anaerob_car-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42002">
        <property type="entry name" value="PTHR42002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03606">
        <property type="entry name" value="DcuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="418" mass="42470" checksum="70C33D29471FB03A" modified="2004-10-11" version="1">MLPWVGVLIVILTVYAILKRYETRLVLFTAGLLMALIALKPMEAFSQFQKSMTNAGLISAICSVMGFAYVMKLTQCDQHLVHAVANTVARARAILIPATTIATFLINIALPSAAGCAAAVGAVMIPVLMAAGVHPAVAAAAVLAGTFGSVLSPGSSHIVMVAEMSGASVVDTIATVTPATVVTGLIGAGVLTILAFLRREDRGHASETVTESPQFRPNPLYALVPIVPLVLLVLGATVPAMKNWRLTVPACMLIGTILALLVTRMNPAEVTKSFFDGMGKAYGDVIGIIIAAGVFAAGLTQVGLIDLLLNAATGARGAIGAAGTWGPFLVAILSGSGDAATIAFNEAVTPHAASFGLTIPKLGTLASLAGSLGRSMSPVAGAAIIVSGIAGVSPIEVAKRNAPGMILASVVAFLMQGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NT4</accession>
    <name>IDI2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="1">IPP isomerase</shortName>
            <ecNumber evidence="1">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="1">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fni</name>
        <name type="ordered locus">STH1674</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195802.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NT4"/>
    <dbReference type="SMR" id="Q67NT4"/>
    <dbReference type="STRING" id="292459.STH1674"/>
    <dbReference type="EnsemblBacteria" id="BAD40659">
        <property type="protein sequence ID" value="BAD40659"/>
        <property type="gene ID" value="STH1674"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1674"/>
    <dbReference type="eggNOG" id="COG1304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065515_0_0_9"/>
    <dbReference type="OMA" id="WDWGIPT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="PTHR43665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229514" description="Isopentenyl-diphosphate delta-isomerase">
        <location>
            <begin position="1"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="270"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <sequence length="363" mass="38117" checksum="C42AB15F019412A4" modified="2004-10-11" version="1">MDLRQQRKRDHVRLAAAWQERRPPPAAAGPGAGWEDVHLVNHSLPELALAEIDLTTSVAGVRLAQPVVINAMTGGADDVTAINRDLAAVAADLGLAMAVGSQTAGLRDPAVADSYRVVRRVNPKGIVLANVGSDATPEQARAAVEMVEADLLQIHLNAPQELRMPEGDRDFRGRLEAIARMVEEAPVPVVVKECGFGVSRDVAVLLHQAGVRAVDVSGRGGTNFAWIEDRRAGLSDPDPGLQNWGIPTACALAEVAALGLPELDLIASGGIRHGSDAAKALALGARAAAVAGPVLLRQQREGARGVMAYLQQFLTDLRAAMLLAGAGSVAAMGQVPVVVTGFTGEWCRLRGVDLMALANRSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NX9</accession>
    <name>Q67NX9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1629"/>
    <dbReference type="EnsemblBacteria" id="BAD40614">
        <property type="protein sequence ID" value="BAD40614"/>
        <property type="gene ID" value="STH1629"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1629"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_603988_0_0_9"/>
    <dbReference type="OMA" id="MERWADY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="209"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="241"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="453" mass="48479" checksum="CF617D746C18386E" modified="2004-10-11" version="1">MERFTYTRTGQALKNKYSSLILLSLADLVAWVIPPLLMPRPAGLWVGAGGLVATLAVYWWLTASLRTCHVLDGDRLILRMGRSALAVDLADVESARMAGHGLRVPGEAGVMYAPRTDTLFMVADHRQLVSVDLSRPYAMKVPGQGICQFTRIVLSLDEPERFLQAVAGRRGQVHLDRPQPAAGGEGRPGATSPAPPVVSEGPAPGGAALALEGLVKRFGGFTAVAGVDLAVRYGEIVAFLGSNGAGKSTTIRMATGLVRPSAGRVLVEGRDLWAEGAPVRRLLGYVPDTPLLYESLTAREFLWLMAGLYGLSRVEGRRRADELLRQVGMERWADYPIRAFSLGMKRKMAIAAALVHRPRVLLLDEVTNGLDPRAAREVKDFIRQAAGEGAAVLLTTHALDVARELADRIAILHGGRLRAVGDLEALRSAAGLPGAGLEELFLALTAEAEGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P71</accession>
    <name>Q67P71_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemotaxis protein CheA</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cheA</name>
        <name evidence="13" type="ordered locus">STH1537</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195667.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1537"/>
    <dbReference type="EnsemblBacteria" id="BAD40522">
        <property type="protein sequence ID" value="BAD40522"/>
        <property type="gene ID" value="STH1537"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1537"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_3_6_9"/>
    <dbReference type="OMA" id="MMDMAKS"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037052">
        <property type="entry name" value="CheA-like_P2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010808">
        <property type="entry name" value="CheA_P2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035891">
        <property type="entry name" value="CheY-binding_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07194">
        <property type="entry name" value="P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55052">
        <property type="entry name" value="SSF55052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="12">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="330"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="11">
        <location>
            <begin position="564"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="9">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD40522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="710" mass="76319" checksum="9930F88D63E3B72D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PE6</accession>
    <name>Q67PE6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195592.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1462"/>
    <dbReference type="EnsemblBacteria" id="BAD40447">
        <property type="protein sequence ID" value="BAD40447"/>
        <property type="gene ID" value="STH1462"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1462"/>
    <dbReference type="eggNOG" id="ENOG502ZVNA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_505184_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="515"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="539" mass="59750" checksum="2012EF73C673DB60" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PK7</accession>
    <name>Q67PK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40386">
        <property type="protein sequence ID" value="BAD40386"/>
        <property type="gene ID" value="STH1401"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1401"/>
    <dbReference type="HOGENOM" id="CLU_069368_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="272" mass="29728" checksum="3175718205A4A73D" modified="2004-10-11" version="1">MDLNGDGRVEGIIGALGEWHRDEVIVVDGGGAPLLILNDTLSSFSQGRVDLVAVPGVADAVLVNQVGDPGDWHSIQFIWMEGDRFVSPQGWLPKNSLAHGQGYTIRPDGTVDITGSLAGYTFVRQYRMEKAPPESSWPYQAVLVSQEVTPGPYPTSAADLLTAVFLARWYGLTDQLEQYIPDPVVREAFLNQEMDEIRYWPLPVQVGRLVEGETGPRIEPASPGDDGAVEFLAVVQQYEGGTYRTGRAVIATAEDGRMVVTDLEILEQGWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PT9</accession>
    <name>Q67PT9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Malate oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1319"/>
    <dbReference type="EnsemblBacteria" id="BAD40304">
        <property type="protein sequence ID" value="BAD40304"/>
        <property type="gene ID" value="STH1319"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1319"/>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034446_2_1_9"/>
    <dbReference type="OMA" id="RTVNTEM"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="476" mass="50995" checksum="D84770535771AADA" modified="2004-10-11" version="1">MAQRLASMRIIVRLEIENTGSIFSYIATAIGASGGDIVAVDLIQSRRDVVVRDITIAVENRAHGDRIVQALAALPGVKVVNVSDRIFLAHLGGKIEVKPRVQVKTRDDLSVVYTPGVADVCRAIAEDPAKAFTLTIKRNTVAVVTDGTAVLGLGNIGPAAALPVMEGKAMLFKQFAGVDAFPICLDTTDPDEIVRIVKAIAPAFGGINLEDISSPRCFEIEERLKEELDIPVFHDDQHGTAVVVLAGLMNAARVVGKELADLRVVVCGIGAAGVAISKMLMAAGITNIVGVDRVGIIERGKTYENPMWQWYAEHTNPENRHGTLDDAIEGADAFIGVSAPNVLKVEHVKKMAKDPIVFAMANPVPEIDPDEAAPYVAVMATGRSDYPNQVNNLLCFPGIFRGALNCRARQINEAMKLAAARAIASVVSDEELHAEYIIPSVFNKRVVEAVAAAVEQAAYETGVARRERQPRTGGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PU5</accession>
    <name>Q67PU5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sugar ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1313</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1313"/>
    <dbReference type="EnsemblBacteria" id="BAD40298">
        <property type="protein sequence ID" value="BAD40298"/>
        <property type="gene ID" value="STH1313"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1313"/>
    <dbReference type="eggNOG" id="COG0395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_1_1_9"/>
    <dbReference type="OMA" id="DIFAWPP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="73"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="282" mass="32236" checksum="438298BD82F36C86" modified="2004-10-11" version="1">MNRKLSDLIKGFLTYFLLTLGAAVIVVPLFWMVSTSLKGSDALFTYPPDWIPRPPVWRNYVDAWRMLPFGRFLLNTLFITVLAMAAELLTTSLVAFGFARFQFRGRDTLFFILLCTMMLPGVVTMIPSFMIWARLKQVDTFVPLTVGAWFAWGPAYIFMMRQFFLTIPREMEEAAIIDGANIVQIYWHIMLPLIRPALLAIGVLAFQGNWNNFQSPLIYLHSTDHFPLILGLKFFEQSLSKEAPMWNYMMAISVLMALPILGLFFAAQRYFIEGLNVGATKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV7</accession>
    <name>Q67PV7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1301"/>
    <dbReference type="EnsemblBacteria" id="BAD40286">
        <property type="protein sequence ID" value="BAD40286"/>
        <property type="gene ID" value="STH1301"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1301"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1160632_0_0_9"/>
    <dbReference type="OMA" id="EMPAGQW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="WYL" evidence="3">
        <location>
            <begin position="103"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="239" mass="26449" checksum="3F17912423BFA961" modified="2004-10-11" version="1">MAIYRKCKYCGAPLSLREMPAGQWVAFDFGTDIVHQCRLAEAQVAKADAMAWPSSNDRSKAGSRPRSGASTRSQASTRNRSRRSAAKALTADARELATEYTAILRLLERAIAERRCVWMRYYTAYRQDVTERVVEPTSLTPAGSGYILEAYCHFRGEYRSFAVGNIRRAQLLNETFTPRPRARGTPEPHYTGPPANRERGQLAERPTEEKAAASAAVCGCLLPILIALLVLILTALLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q00</accession>
    <name>Q67Q00_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dihydroorotase</fullName>
            <shortName evidence="5">DHOase</shortName>
            <ecNumber evidence="5">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrC</name>
        <name evidence="7" type="ordered locus">STH1258</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195389.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1258"/>
    <dbReference type="EnsemblBacteria" id="BAD40243">
        <property type="protein sequence ID" value="BAD40243"/>
        <property type="gene ID" value="STH1258"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1258"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_0_9"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_B">
        <property type="entry name" value="PyrC_classI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="53"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="327"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="434" mass="46290" checksum="B89383947402155E" modified="2004-10-11" version="1">MSSLLIRGGRLLDPANGVDRVADVLVADGRIRAIGEGLPVPPGATVIEASGKVVAPGFIDVHVHLRVPGQEYKEDMRTGTAAAVRGGFVGVVCMPNTRPVIDSAPLVAHVLDLAGREGLCRVWPAGAITKGSQGEELAEIGEMARAGAVAVTDDGKPVDRAETMRQAMIYARQFDLPVMDHCEDRSLSAGGAMHEGRVSSLLGLKGIPAAAEEVHVARDILLALETGARVHLQHLSSRRSVELVRWGKAQGARVTAEATPHHFTLTDQAVADMAYSPNTKMNPPLREEADRQAILEGLADGTIDVIATDHAPHHVDEKDVEYPLAAFGITGLETAVGLALDRLVRPGLIRLEQLILMLSTRPARLINRTPPALREGAEADITVLDLDRTWTVSADEMASKSRNSPFIGWTLTGAPYATVVGGRLRMLEMKVIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q10</accession>
    <name>Q67Q10_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PTS system N-acetylgalactosamine-specific IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">agaC</name>
        <name evidence="8" type="ordered locus">STH1248</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195379.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1248"/>
    <dbReference type="EnsemblBacteria" id="BAD40233">
        <property type="protein sequence ID" value="BAD40233"/>
        <property type="gene ID" value="STH1248"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1248"/>
    <dbReference type="eggNOG" id="COG3715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069101_2_1_9"/>
    <dbReference type="OMA" id="WHMIALA"/>
    <dbReference type="OrthoDB" id="1687744at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004700">
        <property type="entry name" value="PTS_IIC_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03609">
        <property type="entry name" value="EII-Sor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51106">
        <property type="entry name" value="PTS_EIIC_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="267" mass="27799" checksum="4A10E970F5CD0CF6" modified="2004-10-11" version="1">MSFEITALQIILVFIVTFIAAIDQFNFLESLYQPIVTGAVIGAILGDVRTGLIVGGTYQLMTIGNMPIGGAQPPNAVIGGIMAAILAITLGLQPTVAVATAIPFSLLGQYAVTLLFTAMSPVMSYADRAAQEASPGKIVAINYFAMAVLGLLFGVIVTLFFVGGAAFGNRVVSAIPQWLMSGLSAAGGMMRFVGFAILLKVMASRDMWGFYFMGFALANIVAGIPQLSSSALLLLALIGFALAFWDFQHQTALKAAAVSAGGDDDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q80</accession>
    <name>Q67Q80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1178"/>
    <dbReference type="EnsemblBacteria" id="BAD40163">
        <property type="protein sequence ID" value="BAD40163"/>
        <property type="gene ID" value="STH1178"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1178"/>
    <dbReference type="HOGENOM" id="CLU_535193_0_0_9"/>
    <dbReference type="OMA" id="DTLWFAS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269311" evidence="2">
        <location>
            <begin position="37"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="509" mass="50570" checksum="C6C8EBDEB48617F7" modified="2004-10-11" version="1">MRALGRQIRESGSRLPLVLIPVTMVISSLAAGHVTGATFALNQDVGQAQSIQFQALTTSQVLRFTSGRVQLVEDGGPDQVGSEVVRVFRIMNISDRRVDLSVELTGIPWARAALSRSVLGPGETAEIVVSGTPDQPGQYQGYLTVYGMGGFLELGMEFTVVIVPAPDPCRPAEWPAPGARGGEASGDPVREPDPEETEESEAVIGLHPDLLGLPAEDAPEPAAAEPDGEPEAPEAADEAGHEPGEGAGEPAAPGDDGSASESGDEAVPSGDGVDGGAAAPPDTTDPCADAPGGQLPDPAPDCGVPAEAVVEEEEGEQAAEPDDCAPAAAPPGDEAAEPGDGDQDGRKEEDEDAAGEPVEDDAEGTGDPHDDELTEGDTAGEPEDDADDSGSGAEQPGDAVGGTDEAADDGIAGGDGDAGSGETDSDEPDSDGPEDGAPDDGDPGVSDDADGPGAEDADADTGDADGGSPGGEQAHGDSAGDDGADPDDTGQPLTGSDAADDADAGGQTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM7</accession>
    <name>MURD_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murD</name>
        <name type="ordered locus">STH1031</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QM7"/>
    <dbReference type="SMR" id="Q67QM7"/>
    <dbReference type="STRING" id="292459.STH1031"/>
    <dbReference type="EnsemblBacteria" id="BAD40016">
        <property type="protein sequence ID" value="BAD40016"/>
        <property type="gene ID" value="STH1031"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1031"/>
    <dbReference type="eggNOG" id="COG0771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032540_0_1_9"/>
    <dbReference type="OMA" id="KGMHNVE"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000109107" description="UDP-N-acetylmuramoylalanine--D-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <sequence length="455" mass="48635" checksum="AC9D2DC65CA0668B" modified="2004-10-11" version="1">MAGVKLQERAAVVGIGISNMALIRYLVARGVRVTACDRTSAEKLGERAAELARLGVPLVAGEGYLEPLADHDLIFLTPGMPKHLPEIEAARRRGAAISGEIGLVLDLCRAPVIGVTGSAGKTTTTTLIGEILRAAGRETYVGGNIGKPLIEQVEAIPSEAVVVLELSSFQLQLVHRSPHIAVVTNVSPNHLDVHATMEEYVEAKKAIFRRQSPADWLVLNADDPTVRAFAAEARSRVVLFSRRADPGGRDAAFVREDRVIWRRGGRETPILFLDEIRLPGLHNQENVLAAVAACFLAGAPLSAVREVVTQFTGVEHRIEPVRVLDGVKWYNDSKATSPAEAVAALTTLPAPIVLIAGGSDKGIPFDPIAPLVAEKVKTLILMGPTAPKIEEAVRRGGYAGPIRRAADMAEAVALAREAAAPGDTVLLSPACASFDAYRNFEERGRHFKSLVEALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QM8</accession>
    <name>Q67QM8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195162.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1030"/>
    <dbReference type="EnsemblBacteria" id="BAD40015">
        <property type="protein sequence ID" value="BAD40015"/>
        <property type="gene ID" value="STH1030"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1030"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_9"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="31"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="397" mass="43433" checksum="2E2315360BC3802E" modified="2004-10-11" version="1">MRLSARARALTPSPTMAIDAMSKQMVAEGIDVVNFSVGEPDFDTPESIKAAGIAAIQQGETKYTPAAGTLELRKAICEKLERDNGLQYRPEEIVVSNGGKQSLYNAYQVLLDPGEEVIIQAPYWVSYPEIVRLAGGVPVVVETDESTGFRLTADMIREKLTNRTRVINLNSPSNPTGAVLSRRELEEIAALAVEHDLMIVTDEIYEKLLYDGAEHVSIASLGEEVKRRTITVNGLSKAYAMTGWRMGYTASERIYAKAMADLQSQSTSGPSSISQAAAVAALRGSQESVEQMRQEFDRRRRHMLERLNRLPGFSVKVAPAGAFYLFPNVSALFGETIAGRKITSSDDLAEVILEQAHVAVVPGTGFGAPHHIRFSYATSMERIDEGLDRIARLLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR2</accession>
    <name>Q67QR2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum ABC transporter molybdenum-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH996</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein ModA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH996"/>
    <dbReference type="EnsemblBacteria" id="BAD39981">
        <property type="protein sequence ID" value="BAD39981"/>
        <property type="gene ID" value="STH996"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH996"/>
    <dbReference type="eggNOG" id="COG0725">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065520_1_0_9"/>
    <dbReference type="OMA" id="VCAPQVP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13539">
        <property type="entry name" value="PBP2_AvModA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044084">
        <property type="entry name" value="AvModA-like_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004846">
        <property type="entry name" value="ModA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01256">
        <property type="entry name" value="modA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004846-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="253" mass="26154" checksum="C110748D321A1A70" modified="2004-10-11" version="1">MLILVTGCAPRGPGGGGAPGNGAGDAARPLTVAAASDLHFAFSEIGKLFTEETGVEVTFTFGASGSLARQIENGAPVDLFAAADTAYVDHLIEAGAILPDSRRVYALGHLVLAAGAAAPVDDLEDLLDPAIRHLAVANPDHAPYGRAAREALESAGLWEALQPKLVLAENVRQALQYVETGNADAGLVPRSLADVPDITFVAVDPSLYAPLEQTLGIVAGSPREAEARRFAELVTGPEGRAVLERYGFGLPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QR4</accession>
    <name>Q67QR4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH994"/>
    <dbReference type="EnsemblBacteria" id="BAD39979">
        <property type="protein sequence ID" value="BAD39979"/>
        <property type="gene ID" value="STH994"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH994"/>
    <dbReference type="HOGENOM" id="CLU_1958483_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="128" mass="14392" checksum="002052B904179CCF" modified="2004-10-11" version="1">MRPAGRYTEGERGSFAVAGDRWKGATTERGKPRVYIQQYLDLKLPEERYKRICQEMGLEKPFTEEAATAVMEAYLEDLADGSQCDTVHLHRSGGDVVLMTVVSFPYSQAAYDRMMAAYLAKKSLGQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QT8</accession>
    <name>Q67QT8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH970"/>
    <dbReference type="EnsemblBacteria" id="BAD39955">
        <property type="protein sequence ID" value="BAD39955"/>
        <property type="gene ID" value="STH970"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH970"/>
    <dbReference type="HOGENOM" id="CLU_1239613_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="223" mass="24416" checksum="2C266393CA368F99" modified="2004-10-11" version="1">MGMAAVLSSRPEVSREVSMPMPTGGPVRLPAQVAGSMSRMQGLLQAAQPEIQSAYAAHWLAARQAAGRPYFQDLSQHMMMGLYGTTALGGLLRYALSGRATPEVVGGIIDQVQLIRQSYEGAQQALERFLEDEEVRSLSGVQLMSRTLPHLDRFYQQMEQPGQAVLNGIEWQPSEEVQGWPVRRAEVGGPARGWQPPLPRWESPGELDRTGDVSELPPPEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QX9</accession>
    <name>Q67QX9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH929"/>
    <dbReference type="EnsemblBacteria" id="BAD39914">
        <property type="protein sequence ID" value="BAD39914"/>
        <property type="gene ID" value="STH929"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH929"/>
    <dbReference type="HOGENOM" id="CLU_653267_0_0_9"/>
    <dbReference type="OMA" id="HFQYNGW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="436" mass="46622" checksum="B70933064B09E43E" modified="2004-10-11" version="1">MGASSCAVTRVTGPIGCTAVWLKRKVRPDRRSTEMHAANILLRSSARWLAVVLPLGVLIRSAFVWLYTPGPFYWGDLIHAHSHTAYFGWAGLGLMGLILHLLPRFTGRPVAESRPLTWLLRLAPWAVGGALVTFAWWGYAGPSIGFAALNEVLWVLFAVVFWREVRGRPLRDWPAPLVLMGAAVLLLLLSISGTTLVIISEAVLDGAFPLLYQAGVYLFLELYADGWVEIGLMGVVLALAEREAAGGQADEGPAARRVATAALLLVGPAALRMLIPRGLEGPLVWLSIAAGALFGLAQLYFLAIARRLRLPAAAGPWWRLAALSLALKAALEFMPVLPAWSALALDRNPVIAYLHLKLLLLASSGLLGALAAVRGAAEPWSFRLYAAGSVVMVAALAAHGLLARAIPAVNWPLYAVAFAAAFPAAAGGIWGAQPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QY4</accession>
    <name>Q67QY4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH924"/>
    <dbReference type="EnsemblBacteria" id="BAD39909">
        <property type="protein sequence ID" value="BAD39909"/>
        <property type="gene ID" value="STH924"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH924"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_9"/>
    <dbReference type="OMA" id="FHAGIHY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="22"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="18302" checksum="710F749030579968" modified="2004-10-11" version="1">MRYRWGAAPRPFPNWRRRWVVKKILLATDGSESSLKAARIAGELASLQNAQVIVLYVAHVPRAYYATDEFGNKVTPEVPMDVLIRRTGEPILRKTVSAIGLPESQVVTEVQVGEPAEEIVDYARLEEVDLIVMGSRGLSPIRELLLGSVSDKVLRTAPCPVLIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R27</accession>
    <name>Q67R27_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-coenzyme A synthetase</fullName>
            <shortName evidence="5">AcCoA synthetase</shortName>
            <shortName evidence="5">Acs</shortName>
            <ecNumber evidence="5">6.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acetate--CoA ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Acyl-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acsA</name>
        <name evidence="9" type="ordered locus">STH881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="5">Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="5"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195013.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="BAD39866">
        <property type="protein sequence ID" value="BAD39866"/>
        <property type="gene ID" value="STH881"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH881"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_9"/>
    <dbReference type="OMA" id="HAAIRWE"/>
    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046872">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
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        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACAS_N" evidence="8">
        <location>
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            <end position="83"/>
        </location>
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        <location>
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            <end position="527"/>
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        <location>
            <begin position="536"/>
            <end position="614"/>
        </location>
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    <feature type="region of interest" description="Coenzyme A binding" evidence="5">
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            <end position="196"/>
        </location>
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        <location>
            <position position="315"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
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        <location>
            <begin position="391"/>
            <end position="393"/>
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        <ligand>
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            <begin position="415"/>
            <end position="420"/>
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        <ligand>
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        </ligand>
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            <position position="505"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
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        <ligand>
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        </location>
        <ligand>
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            <position position="542"/>
        </location>
        <ligand>
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        <location>
            <position position="544"/>
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        </source>
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        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="BAD39866.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="654" mass="73396" checksum="4E62FEE8F0CCBDC8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD2</accession>
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        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
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        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
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        <source>
            <dbReference type="EMBL" id="BAD39761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="145" mass="15894" checksum="40DCC8CB1F24107F" modified="2004-10-11" version="1">MLLEIISEAVVGRAEHTFTWTHTLPADGVVQVLGVRPGPSEARVRAEGGSPQAEVTVDVDLWFLGQDGTRVTRTRCQTVQPAPVALRGNLLSDPSYDLRLLGNARTTDVRVEDGSVHLDMAVTVEVEARALTRYWVRAEDHVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RI4</accession>
    <name>Q67RI4_SYMTH</name>
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        <submittedName>
            <fullName evidence="2">Stage V sporulation protein R</fullName>
        </submittedName>
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        <name evidence="2" type="primary">spoVR</name>
        <name evidence="2" type="ordered locus">STH724</name>
    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Clostridia</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
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    </dbReference>
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        <location>
            <begin position="163"/>
            <end position="213"/>
        </location>
    </feature>
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        <location>
            <begin position="163"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="453" mass="53300" checksum="463F4AAF305DBBE7" modified="2004-10-11" version="1">MDDRDLPALEEAIGEIWETARRLGLDPFPTHFEIVPATILYEFGAYLLPGRFSHWTHGKAYYTMKSSYDYGLSKIYELVINANPSYAFLLETNSLLQNKFVVAHVFGHTDFFKNNIWFAPTNRQMLETVSQNAQRIRQYGIEEGDLEVERFLDAVLSISDHIDPYPRREAPEPEREPPPSAHDGPYADLFPREETGERKAGGKPGKPARRRIPPAPEKDLLLFLLEHADHLEDWQRDIIAIVREESLYFVPQMQTKIMNEGWASFWHMRIMRELDLTDEEFVEFGKLHSAVCTPGHTRINPYYVGLKIFEDIEKRFGREKIFEVREMENDVSFLRTYLTEELCRDLDLFVYELKDDAWKITDKQWERVRDALCDAMTNFGHPYIVVEDGDYRGRRELLLRHQHDGRDLDLPYAEKTMQYLYQLWGRPVHLETQVEGRRTRITCEGEKVTMEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM0</accession>
    <name>Q67RM0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH688</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194821.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH688"/>
    <dbReference type="EnsemblBacteria" id="BAD39673">
        <property type="protein sequence ID" value="BAD39673"/>
        <property type="gene ID" value="STH688"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH688"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_439997_0_0_9"/>
    <dbReference type="OrthoDB" id="1433825at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="621" mass="64947" checksum="C96E68577EBAC763" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RQ5</accession>
    <name>Q67RQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH653</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194786.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH653"/>
    <dbReference type="EnsemblBacteria" id="BAD39638">
        <property type="protein sequence ID" value="BAD39638"/>
        <property type="gene ID" value="STH653"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH653"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_9"/>
    <dbReference type="OMA" id="ETACEEM"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="28108" checksum="E381B1CCB9FD0C5E" modified="2004-10-11" version="1">MAITAESVRFGYRGGEVIRGVSFTVPEGAITCLLGRNGSGKTTLIRLVNGILRPHDGSIRIDGMDVLRSPRAAVARRVAFVPQEHQGVFPYGVEELVVMGRNPHLGPVARPGRSDRAAARAAMEAVGIAHLADRDYMQISGGERQLVLIARALTQETPYLLMDEPTSHLDFTNQHAILSIVRRIARERGVGVLVAMHDPNLALAFADRVILLREGRVFQAGPAAEVMTPENLQTLYQMPIDVVRLPDGRRLIVTEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT1</accession>
    <name>Q67RT1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194760.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH627"/>
    <dbReference type="EnsemblBacteria" id="BAD39612">
        <property type="protein sequence ID" value="BAD39612"/>
        <property type="gene ID" value="STH627"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH627"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_1_1_9"/>
    <dbReference type="OMA" id="RMFIPRE"/>
    <dbReference type="OrthoDB" id="1606021at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006504">
        <property type="entry name" value="Tscrpt_reg_Spx/MgsR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01617">
        <property type="entry name" value="arsC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="121" mass="13938" checksum="5BEDDE5AF10ACE15" modified="2004-10-11" version="1">MKPEVTFFTYPTCTSCKKAKQLLDEKPVEVHERRFFKEKPTPEEVRWLAARLPGGVRDLLSTRSRRYKELGLAERELTDDELVELLAAEPGLWRRPVVVRGDQVVVGYDPASLEELLRVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RT3</accession>
    <name>Q67RT3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ThrE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH625"/>
    <dbReference type="EnsemblBacteria" id="BAD39610">
        <property type="protein sequence ID" value="BAD39610"/>
        <property type="gene ID" value="STH625"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH625"/>
    <dbReference type="eggNOG" id="COG2966">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070277_0_0_9"/>
    <dbReference type="OMA" id="HNEAYPR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE" evidence="2">
        <location>
            <begin position="12"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="25679" checksum="C01C6E8C00AA60C8" modified="2004-10-11" version="1">MGSGSATAALQVAAAAGVVLLQSGADVARVEDTVSRILRAYGVGEPEIYATPTGIFISVADQEATVVRRVRSRTIALDRVSAVNALSRSLTADPKDPAEALRLIRAIGEQPPPFPRWLDVPASGLAAAACTMLVGGSLAGVLPAFLANLVVQAGERICRWLGLPDAVADLISGANAVFCATLLHRWLGVPVGPVVAGGIMILVPGIAFTNALRDAIAGDLVSATARGLEAFVKVTALAAGVGAALYLVGGGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ6</accession>
    <name>Q67RZ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tagatose-6-phosphate aldose/ketose isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH562</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-galactosamine 6-phosphate + H2O = D-tagatopyranose 1-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:47680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:71674"/>
            <dbReference type="ChEBI" id="CHEBI:138150"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. AgaS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH562"/>
    <dbReference type="EnsemblBacteria" id="BAD39547">
        <property type="protein sequence ID" value="BAD39547"/>
        <property type="gene ID" value="STH562"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH562"/>
    <dbReference type="eggNOG" id="COG2222">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012520_0_0_9"/>
    <dbReference type="OMA" id="YHLIITC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05010">
        <property type="entry name" value="SIS_AgaS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035464">
        <property type="entry name" value="SIS_AgaS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SIS" evidence="5">
        <location>
            <begin position="40"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="5">
        <location>
            <begin position="216"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="386" mass="42223" checksum="BFE281DBC4020113" modified="2004-10-11" version="1">MEELKRLGAALTAREIAQQPELWAETFATYRREQARFEGFLQTIRERHGRVRVLFAGAGSSAYVGETVLPFVKRHGDEHAFEFQVTPTTSLVADPTSYLRADQPTLLVSFARSGNSPESVATVHLAKQLVPDLYQIIITCAPDGNLARQADGDERSLLWLMPQRANDQAFAMTGSFTCMVLAALLLFDRRPLAEKERYVSQICALGRSVIEREAEIAALAGLDFGRIVYLGSGPLAGIAREVQLKILELTAGRIATLFDTSLGFRHGPKSFVDEQTLVFVLISNDPYTRKYDLDMLRELHDDGIARLACGVLADDGDGPAAPTFGFPAAYAALPDGYLALPFALFGQILALHTAVKLGNTPDTPNPRGTVNRVVKGVTIHEFPQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S06</accession>
    <name>Q67S06_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphopentomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphopentomutase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194686.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH552"/>
    <dbReference type="EnsemblBacteria" id="BAD39537">
        <property type="protein sequence ID" value="BAD39537"/>
        <property type="gene ID" value="STH552"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH552"/>
    <dbReference type="eggNOG" id="COG1015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053861_1_0_9"/>
    <dbReference type="OMA" id="HQEIMGT"/>
    <dbReference type="OrthoDB" id="1772797at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043094">
        <property type="term" value="P:cellular metabolic compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16009">
        <property type="entry name" value="PPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010045">
        <property type="entry name" value="DeoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024052">
        <property type="entry name" value="Phosphopentomutase_DeoB_cap_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21110">
        <property type="entry name" value="PTHR21110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001491">
        <property type="entry name" value="Ppentomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="5">
        <location>
            <begin position="267"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="406" mass="43320" checksum="AD8CE6701FC0FA57" modified="2004-10-11" version="1">MAKRAIVLVLDGFGVGAMADVEQVQPRDAGAHTLASLVRSQGALRIPSLVRLGLPHIAPEAGLEPAGPPLAAWGRCNLAHWGADTFAGHNEIQGNRPLRPVISLFSTVAGQVRAHLEQAGHRVEDALPGGSALLVDGQILVGDNLETDPGRIYNVTGPLDTVPFERILEVGRRVRQVVQVSRVIALGGVGITVEDILAHTRITPAGQTGVVTPELHIYNEHYRVRHLGYGIDPAVQAPELVASTGLPVAMIGKMADVVECPSARWRDPVVDTEAVLEQVLEQLEQMPDGGYIAATVQETDLMGHEQDPVRYAEVLARADRGIARLLDRLRPGDLLIILADHGNDPLIGHSLHTREQVPLLAFGPGFRPAALGVRDTMADVAATVCEHLGAPAPEFGTSFLGLLTEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S53</accession>
    <name>DNAJ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaJ</name>
        <name type="ordered locus">STH505</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaJ family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S53"/>
    <dbReference type="SMR" id="Q67S53"/>
    <dbReference type="STRING" id="292459.STH505"/>
    <dbReference type="EnsemblBacteria" id="BAD39490">
        <property type="protein sequence ID" value="BAD39490"/>
        <property type="gene ID" value="STH505"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH505"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017633_0_7_9"/>
    <dbReference type="OMA" id="DLHCTVT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000070910" description="Chaperone protein DnaJ">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="152"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="195"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif">
        <location>
            <begin position="209"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="139"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <sequence length="386" mass="42541" checksum="E579253D353BA3FF" modified="2004-10-11" version="1">MDRLAKRDYYEILGVPRNATEAEIKKAFRNLARKYHPDANKDDPDAAEKFKEINEAYQVLSDPEKRARYDQFGHAAEQMGGAGGNPFEGFGGFGDFGGFSDIFEMFFGGAGRQRNPRGPVRGADLEYELELTLKEAAFGCTKELRVPRVEDCDTCHGSGARPGTQPVTCPKCGGTGQVQMTQHTVFGRFVNVMTCDRCRGEGKIVESPCPTCRGRGRVQKTQRVEVKVPGGVETGTRLRMPGYGEAGERGGPPGDLFIVMRVRPDRRFRREGDDLFTTTEISFIQAALGTEIEVETLDGPELIKIPEGTQPGDTIRLKGKGTHRLRGSGRGDLHVVISVKTPGRLSERERELLLEVAALRGERVAGMTENKEKSFLKKMKDALGGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S57</accession>
    <name>GUAA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaA</name>
        <name type="ordered locus">STH501</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194635.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67S57"/>
    <dbReference type="SMR" id="Q67S57"/>
    <dbReference type="STRING" id="292459.STH501"/>
    <dbReference type="MEROPS" id="C26.957"/>
    <dbReference type="EnsemblBacteria" id="BAD39486">
        <property type="protein sequence ID" value="BAD39486"/>
        <property type="gene ID" value="STH501"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH501"/>
    <dbReference type="eggNOG" id="COG0518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_9"/>
    <dbReference type="OMA" id="KRKIIGH"/>
    <dbReference type="OrthoDB" id="504464at2"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000229478" description="GMP synthase [glutamine-hydrolyzing]">
        <location>
            <begin position="1"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase" evidence="1">
        <location>
            <begin position="199"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="226"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <sequence length="513" mass="57567" checksum="A81ED8589FF6554B" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SA3</accession>
    <name>Q67SA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH455"/>
    <dbReference type="EnsemblBacteria" id="BAD39440">
        <property type="protein sequence ID" value="BAD39440"/>
        <property type="gene ID" value="STH455"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH455"/>
    <dbReference type="eggNOG" id="COG1555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052011_1_0_9"/>
    <dbReference type="OMA" id="MVDIKGA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004509">
        <property type="entry name" value="Competence_ComEA_HhH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00426">
        <property type="entry name" value="TIGR00426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="230" mass="23092" checksum="05EF3497DC144954" modified="2004-10-11" version="1">MAALILLSVLGLGWRAIVLYQADQRALKAAAFLAQLEAGAAGSAADASPAVAGGTPGGAPDEGAGAPPARQVIVHVEGAVQRPDIYALPEGARVNDAILAAGGPLPDAVPGVLNLAAPLVDGAKIYVYREDELQPQAPPPPKAQGATYQPVTSTLGGSGAAAGSGGTTLVNINTAGQAELETVPGIGPALARAIITYRTEHGPFQSVDDLINVSGIGSKTLEKIRPYVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI2</accession>
    <name>Q67SI2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Septum site-determining protein MinD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cell division inhibitor MinD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">minD1</name>
        <name evidence="8" type="ordered locus">STH376</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194510.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH376"/>
    <dbReference type="EnsemblBacteria" id="BAD39361">
        <property type="protein sequence ID" value="BAD39361"/>
        <property type="gene ID" value="STH376"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH376"/>
    <dbReference type="eggNOG" id="COG2894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_1_9"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010223">
        <property type="entry name" value="MinD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01968">
        <property type="entry name" value="minD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="7">
        <location>
            <begin position="5"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="268" mass="29299" checksum="4F65FC711824651C" modified="2004-10-11" version="1">MGQVIVVTSGKGGVGKTTTTANLGTALAQLGNRVVLVDADIGLRNLDVVMGLENRIVYDLVDVVEGNARLKQALIKDKRNENLYLLAAAQTREKKDVTAEQMRDLTEQLAREFDFVLVDCPAGIEDGFKNAIAGAQKAIIVANPEVSSVRDADRVIGLWDAQDGDRSPAMLIVNRVRPRMVARGDMLEIDDMLEMLAVDLLGVVPEDDHIIVSTNRGEPAVYSRDSKAGKAFQNIARRLMGEAVPIMDLETEESLWSRFKRIVGLGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SI9</accession>
    <name>Q67SI9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH369"/>
    <dbReference type="EnsemblBacteria" id="BAD39354">
        <property type="protein sequence ID" value="BAD39354"/>
        <property type="gene ID" value="STH369"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH369"/>
    <dbReference type="HOGENOM" id="CLU_166657_4_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025470">
        <property type="entry name" value="DUF4321"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14209">
        <property type="entry name" value="DUF4321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="8329" checksum="7AB7CA3351EFF4CA" modified="2004-10-11" version="1">MKRGWLVFMWALIGAVVGQLFGAALAHYVPFLNYHIPLGMAPATIDLNFLVVTFGVSFKLTVGGAIGLVLALWLALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SN7</accession>
    <name>Q67SN7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribonucleoside-diphosphate reductase subunit beta</fullName>
            <ecNumber evidence="3">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH321</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 5">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="3 5">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="3"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194455.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH321"/>
    <dbReference type="EnsemblBacteria" id="BAD39306">
        <property type="protein sequence ID" value="BAD39306"/>
        <property type="gene ID" value="STH321"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH321"/>
    <dbReference type="eggNOG" id="COG0208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035339_1_0_9"/>
    <dbReference type="OMA" id="KVGEYQR"/>
    <dbReference type="OrthoDB" id="1384440at2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="PTHR23409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000355">
        <property type="entry name" value="NrdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000355-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="40511" checksum="6C1B6EBE93CDD1ED" modified="2004-10-11" version="1">MERLAERKRLFNEHGERDWGKRRIIGGNPTNIMELTNVKYEWAQKLSDIMLRNFWIPEEVPLLDDAKQYLELTADEQWAYDRMLSFVIFMDSILTVSMPNLINYVTAPEINMCLSIHNFHEALHSKSYGYVLESVVPAERRQYIYDLWREDRQLLERNRYIANTFQLFADNPTDEHFVRACMAQYVLEGIYFYSGFAFFYALARMGKMKGTATEIQFINRDEITHVAVFQNILNGLRQERPQLFTPQLDADLRAMIRQAAEHEMAWAAYCVGNRIQGLSGEVLQGYVKHLADQRARALGFEPLYGNHPNPIKWVESFSNLNQTKADFFETRVTNYAKSTNIDLDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SR2</accession>
    <name>Q67SR2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligoeptide ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH296</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194431.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH296"/>
    <dbReference type="EnsemblBacteria" id="BAD39281">
        <property type="protein sequence ID" value="BAD39281"/>
        <property type="gene ID" value="STH296"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH296"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_9"/>
    <dbReference type="OMA" id="PRHTVYD"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="340" mass="37927" checksum="4BA091C41C00B10F" modified="2004-10-11" version="1">MSLVSLENVSVYFKSERNPREQVGALVGVNLSIEPGEIIAVVGESGCGKTTLGKVITDIYQPTEGRVLYKGKELRKLTKQEYKEYRLGVQMIHQDSYAALNPNRTIFQSLSLPLLQHRLAHNQHDAWDQLSELFVEIGLTPPAQFLQKYPHQLSGGQRQRILLARALSVKPTLIVADEPVSMVDVSLRISLLDLMARANQKLGVSFVYITHDLGTARYIAHHGRIAVMYLGRMVEINQIQAALDHPAHPYFHALISAVPEADPTLRGERLTQLPLRSFDMPSVTNAPPGCKFHPRCPYAQEICEREEPKLREFEGGLVACHLAESIRAGMNGRFSTSSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SU5</accession>
    <name>Q67SU5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194398.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH263"/>
    <dbReference type="EnsemblBacteria" id="BAD39248">
        <property type="protein sequence ID" value="BAD39248"/>
        <property type="gene ID" value="STH263"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH263"/>
    <dbReference type="HOGENOM" id="CLU_2669738_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="75" mass="8572" checksum="69135F74E8DDB864" modified="2004-10-11" version="1">METDPEYRATWQRVRLVEHALEVLTPVERQLIQAIYIDQTHNLVGAALLIGYSRAQTARIRQRALARLAMAMGLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SV5</accession>
    <name>METN_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine import ATP-binding protein MetN</fullName>
            <ecNumber evidence="1">7.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metN</name>
        <name type="ordered locus">STH253</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-methionine(out) = ADP + H(+) + L-methionine(in) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.4.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-methionine(out) + H2O = ADP + D-methionine(in) + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29767"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57932"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.4.2.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetI) and a solute-binding protein (MetQ).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.11" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194388.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67SV5"/>
    <dbReference type="SMR" id="Q67SV5"/>
    <dbReference type="STRING" id="292459.STH253"/>
    <dbReference type="EnsemblBacteria" id="BAD39238">
        <property type="protein sequence ID" value="BAD39238"/>
        <property type="gene ID" value="STH253"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH253"/>
    <dbReference type="eggNOG" id="COG1135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_3_9"/>
    <dbReference type="OMA" id="VFITHEI"/>
    <dbReference type="OrthoDB" id="1058434at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033232">
        <property type="term" value="F:ABC-type D-methionine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03258">
        <property type="entry name" value="ABC_MetN_methionine_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026253">
        <property type="entry name" value="ABC_MetN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041701">
        <property type="entry name" value="MetN_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF5">
        <property type="entry name" value="PTHR43166:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51264">
        <property type="entry name" value="METN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000270431" description="Methionine import ATP-binding protein MetN">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01719"/>
        </source>
    </evidence>
    <sequence length="332" mass="36364" checksum="8C5C7B32B1E60B0F" modified="2004-10-11" version="1">MIELKGLTKVFRQNGQEIVALSDVNLHVPRGQIFGILGQSGAGKSTLIRCVNLLERPTAGEVWVDGREITRLSPGELRAARREMGMIFQQFNLFDSRTVFGNVAYPLEVAGWPRARIRERVEELLALVGLADKAGAYPNQLSGGQKQRVGIARALAPGPKLLLSDEATSALDPDTTRSVLALLREINRRLGLTILLITHQMEVVKQVCDSVAILEEGKVVEQGGVLELIGRPGSRLRELFYESTEEAAHRIHPDGVRVLLFFVGASADRPLISETVRRFRVDANILQGAVERIAGATVGRLLVEFTGLPEDIQDALRFLEEQGASPEVIPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX3</accession>
    <name>Q67SX3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate ABC transporter phosphate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194370.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH235"/>
    <dbReference type="EnsemblBacteria" id="BAD39220">
        <property type="protein sequence ID" value="BAD39220"/>
        <property type="gene ID" value="STH235"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH235"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026228_5_1_9"/>
    <dbReference type="OMA" id="RAISPDM"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269966" evidence="1">
        <location>
            <begin position="16"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="29"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="259" mass="26867" checksum="407892D531644622" modified="2004-10-11" version="1">MRVRVKPLTLVVALAAALLVGCGRPSVGLRIAGSTSLQPLAEMLAEGYRAAGGARVSTQGVGSTAGLQALRSGVADLATVSRRLTPEEMREGFQAHVIAWDVLAIAVHPSNSVDGLTLAELRRLFAGEIRDWAQLGGTAGRVHLISREAGSGSRQAFRELVGPIHPLAAVQNTNGAIRLAVRNDPAAVGYLSLGVARAGGVKLLRIDGYLPGEPGYPLVRPLSLVTLGPPAGEARAFLAYALSPAGQKLVADEGMLPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T00</accession>
    <name>Q67T00_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="8">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lysS2</name>
        <name evidence="8" type="synonym">lysS</name>
        <name evidence="10" type="ordered locus">STH208</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="8"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH208"/>
    <dbReference type="EnsemblBacteria" id="BAD39193">
        <property type="protein sequence ID" value="BAD39193"/>
        <property type="gene ID" value="STH208"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH208"/>
    <dbReference type="eggNOG" id="COG1384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anticodon_2" evidence="9">
        <location>
            <begin position="402"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="35"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="287"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="509" mass="58530" checksum="F48FABCB10C54B65" modified="2004-10-11" version="1">MMSNYPNLWPYQEAQRIIERWPDKPRYILETGFGASGHPHMGTIGEVVRTFYVAKALREMGRESEIIVFSDDMDGLRKIPTNIDAPWLQEHLGKPVTRIPDPYGCHQSFGHHMDAELLEMMAKTEIQATFRSATDCYADGTFDEGIRHMFRHMQGIIDLVLPTMRPENREGWFPWLPVCENCGKVYTTRVESYDPDAVTVTYSCTGSFRDVHGCGYRGTQSALGGRGKFFWKADWALRWYVFGVNFEMHGKDLIDSAKLSKAIVRLLGGDPPNTMFYELFLTEEGRKISKSSGIGISMENWGRWGTQDSLNLLMFRNPRQQKRLSPETVIQYTDETLTMSPEDPQYPFIYYHGDRPDLGGLRYSDIINLVNAISETDPDVLLEYLGRSFGHERIRANEPFVRELLEKAIHYYEDFILPTRRTPELTEAEWALVEEFEQLIERTDDAEEIQTGVFDIARAHGMQPKRFFQILYGVLLGSDSGPRIGGFVTLVGKDRIRELIRTAREKARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T89</accession>
    <name>Q67T89_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH119"/>
    <dbReference type="EnsemblBacteria" id="BAD39104">
        <property type="protein sequence ID" value="BAD39104"/>
        <property type="gene ID" value="STH119"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH119"/>
    <dbReference type="eggNOG" id="ENOG5033P8J">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2083668_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="117" mass="12383" checksum="D156B03BEBFD6005" modified="2004-10-11" version="1">MSAVARAAELIGRLRPSGDPADEPAACVVLVRDQADRIEGALDRIARAADGELVLVDLGSRDETAAILNRLACRYGHALVLQLGHLTRQEAIARAVAATTAPRVVIVDLEGHLEKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TG6</accession>
    <name>Q67TG6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH42</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194177.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH42"/>
    <dbReference type="EnsemblBacteria" id="BAD39027">
        <property type="protein sequence ID" value="BAD39027"/>
        <property type="gene ID" value="STH42"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH42"/>
    <dbReference type="eggNOG" id="COG1432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_936216_0_0_9"/>
    <dbReference type="OMA" id="DYENIWT"/>
    <dbReference type="OrthoDB" id="990092at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="3"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="264" mass="30240" checksum="1D8E2AE4EFEA1F6B" modified="2004-10-11" version="1">MRSVIFMDYENIYWSMKQQYGVAPDLDRLITSLREMGERGNGQVCLMHAYADFDHEEFRGLMSDLQRRSVEPRHVFSKNYEDGTRKNAADIEMSLDALEIMYTRSDIDTFVLVCGDRDMIQVIRKLRARGKQVHVVAVEKTMSKDVMSFVNQFTTIEALLGMLPPAMYDMEMMIRKLDSAECGKPFVGLKYFLRILAGSDIVDRKTYELVNQAISEGIIDTYKVPNPKDELFPTTACKLNREHELVKRVLGEKAQKGQPGTVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI7</accession>
    <name>Q67TI7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfate transporter family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH21</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194156.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH21"/>
    <dbReference type="EnsemblBacteria" id="BAD39006">
        <property type="protein sequence ID" value="BAD39006"/>
        <property type="gene ID" value="STH21"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH21"/>
    <dbReference type="eggNOG" id="COG0659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_13_1_9"/>
    <dbReference type="OMA" id="VTNKFPI"/>
    <dbReference type="OrthoDB" id="751256at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="PTHR11814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13466">
        <property type="entry name" value="STAS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="390"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="484" mass="49704" checksum="B05F74932EFA9FDD" modified="2004-10-11" version="1">MKERNVAGFFSRMTEHWRGDLFGGVTAAVVALPLALAFGVLSGAGPMAGLYGAVFTGILAAIFGGSPAQVSGPTAGMTVVLVPIYQEFGLTGLYMAMFLGSALLLLLSFLKVGRYVHLISQPVLSGFTNGIALLIFLQQIDSLRGASGAIVPAEAGLAGAVVAIMLVWPRITRKVPGSLVALVLSTAAVRLFWPEVFTTIGPIPGGWPTPQLAFFGELANLGALLRPALTIALLGAVESLLSAVVVDEMAGTRHDPDRELFGQGLGNMVATLFGGIVGTGAIVRSAARIQAGGRTRLAAVTHGLVILLVAVALGDLAGLIPRATLAGILMGTAINMVDRRSLADVRRVPVGDAAIMLTTAAITVVFDLVTAVAVGVLLSMIRFAVTVTDAPLTVKRMGKVTAIRLTGPLYFGAAKPFLDAVDAVPEGSTLVLDLRGVTSLDATGAQALERGYARATARGCEVSGPGCSPRFTGCCPAGTGCRCS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TI9</accession>
    <name>SYR_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">STH19</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194154.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TI9"/>
    <dbReference type="SMR" id="Q67TI9"/>
    <dbReference type="STRING" id="292459.STH19"/>
    <dbReference type="EnsemblBacteria" id="BAD39004">
        <property type="protein sequence ID" value="BAD39004"/>
        <property type="gene ID" value="STH19"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH19"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_0_1_9"/>
    <dbReference type="OMA" id="YEFKWER"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000242103" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="558" mass="63061" checksum="BA0FA42459141ED8" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J52</accession>
    <name>RL9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplI</name>
        <name type="ordered locus">STH3317</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67J52"/>
    <dbReference type="SMR" id="Q67J52"/>
    <dbReference type="STRING" id="292459.STH3317"/>
    <dbReference type="EnsemblBacteria" id="BAD42298">
        <property type="protein sequence ID" value="BAD42298"/>
        <property type="gene ID" value="STH3317"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3317"/>
    <dbReference type="eggNOG" id="COG0359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078938_3_0_9"/>
    <dbReference type="OMA" id="MKIILTH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00651">
        <property type="entry name" value="RIBOSOMAL_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000236600" description="50S ribosomal protein L9">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <sequence length="146" mass="15884" checksum="BCCCC114B6C9E7DA" modified="2004-10-11" version="1">MILKADVKGTGKKGQTVEVADGYARNYLIPRGLAVAASEGALRSIEAERKAQQEKQQRQVAELSALRDRLDGQTIQLRAKCGEGGRLFGSVTNKDVADAIARHIGKPFDRKMVELDAPIKTLGVHLVTLRFGHNITGKVNVEVLPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J55</accession>
    <name>PURA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">STH3314</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011197425.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
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    <dbReference type="AlphaFoldDB" id="Q67J55"/>
    <dbReference type="SMR" id="Q67J55"/>
    <dbReference type="STRING" id="292459.STH3314"/>
    <dbReference type="EnsemblBacteria" id="BAD42295">
        <property type="protein sequence ID" value="BAD42295"/>
        <property type="gene ID" value="STH3314"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3314"/>
    <dbReference type="eggNOG" id="COG0104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_9"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="232152at2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
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        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
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    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
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    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
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        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
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        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224328" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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        </ligand>
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        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>IMP</name>
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        <location>
            <begin position="298"/>
            <end position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
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    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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            <position position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
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        <location>
            <begin position="330"/>
            <end position="332"/>
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        <ligand>
            <name>GTP</name>
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        <location>
            <begin position="417"/>
            <end position="419"/>
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        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="432" mass="47444" checksum="C00EECC8876CDE0A" modified="2004-10-11" version="1">MPVVVVMGAQWGDEGKGKFVDLLAERAQVVVRSTGGSNAGHTVWAGGRQYKLHQVPSGILYPGTLCVIGHGVVLDPPKLLEEMDRLASQGVDLSSLRISGGAHIVFPFHIRLDEAEEDRKGDRKIGTTRRGIGPAYMDKFARVGIRLVDMLDRDEFLPKLTALVEEKNRILEKVYGLPGFTVEEIAEPYLEYAERLRPYVANTVELVNDAIDAGKNVLFEGAQGHLLDIDFGTYPYVTASHPIAAGAIIGAGVGPTKVSRVVGVVKAYTSRVGEGPFPTELHGEEAHRIREEGHEYGTTTGRPRRIGWLDLVMVRYACRVSGITDLAVPHLDTLAKTGLPTLKVCVGYRMPDGTVTREFPVGLKALSQVEPVYEELPNWEWSAEMSTARQYDELPEGARRYVRLIEDVTGVRVSILGVGSERTQAIYRSAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J67</accession>
    <name>Q67J67_SYMTH</name>
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        <submittedName>
            <fullName evidence="2">Putative flavin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42283.1"/>
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    </dbReference>
    <dbReference type="RefSeq" id="WP_011197414.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3302"/>
    <dbReference type="EnsemblBacteria" id="BAD42283">
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        <property type="gene ID" value="STH3302"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3302"/>
    <dbReference type="eggNOG" id="COG0702">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_4_5_9"/>
    <dbReference type="OMA" id="EQPEHYG"/>
    <dbReference type="OrthoDB" id="948454at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="207" mass="22732" checksum="212D43F486768255" modified="2004-10-11" version="1">MKIAVIGATRGIGLEVVKQALEDDHDVTALVRDPDRMPVRHPRLHLVQGDARDPESVATAVHGQDVVCDCLGTKNVFARTTLFSTCAQNLARALRPEQLLIAVTGIGTGDSRGHGTFLYDHVVLPLVLGRIYADKERQERIIRDHIERWIIVRPGILTNGPRTGRYRALVDLHGVRGGRISRADVADFVLSQAKSPTFIGKTPLLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JC6</accession>
    <name>Q67JC6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3242"/>
    <dbReference type="EnsemblBacteria" id="BAD42224">
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        <property type="gene ID" value="STH3242"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3242"/>
    <dbReference type="HOGENOM" id="CLU_2653161_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="76" mass="7858" checksum="EA9D6F4EB3862B3B" modified="2004-10-11" version="1">MAHRPASWFRNVAVAGLVVALAMVPAIAALLHGALPLWLHIPLTVLALSLLALGSAAAGVAAVEFRQRQQPAKSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JF6</accession>
    <name>Q67JF6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH3212</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197325.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3212"/>
    <dbReference type="EnsemblBacteria" id="BAD42194">
        <property type="protein sequence ID" value="BAD42194"/>
        <property type="gene ID" value="STH3212"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3212"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_9"/>
    <dbReference type="OMA" id="AHMCDRV"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD42194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="258" mass="28291" checksum="CA7A281E7342F47B" modified="2004-10-11" version="1">MIVLEAKNLTKVYGGRGKVATRALNNLSLQVQKGEFLGIMGPSGSGKTTLLNLLATIDRPTSGTVAIEGTDVSQLRGEQLALFRRRRLGFVFQDYNLLDSLTLGENIALPLVLDRVPVRIIKERLRMLAGQLGIGDILHKYPYEVSGGQQQRAAAARAIIHEPAVILADEPTGNLDSRSAKDLMETLVMLNNMAGVTIVMVTHDAFAASYCRRIQFIRDGQIYTEIRRGDSREAFFQQILDVLALMGGGRDEPLANRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH8</accession>
    <name>Q67JH8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formiminotetrahydrofolate cyclodeaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH3190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197303.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3190"/>
    <dbReference type="EnsemblBacteria" id="BAD42172">
        <property type="protein sequence ID" value="BAD42172"/>
        <property type="gene ID" value="STH3190"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3190"/>
    <dbReference type="eggNOG" id="COG3404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088419_0_0_9"/>
    <dbReference type="OMA" id="VAMVARY"/>
    <dbReference type="OrthoDB" id="1965647at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007044">
        <property type="entry name" value="Cyclodeamin/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036178">
        <property type="entry name" value="Formintransfe-cycloase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04961">
        <property type="entry name" value="FTCD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101262">
        <property type="entry name" value="SSF101262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="FTCD_C" evidence="2">
        <location>
            <begin position="7"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD42172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="22749" checksum="8AAB83D833E95166" modified="2004-10-11" version="1">MRIGEQTVKGFCEQVAASTPAPGGGTVAAVAGAMGASLVAMVAGLTRGREKFRDVEADMAAAQEAGLKEAEALLGLADQDQAAFNQVMAAFALPKGTPEEKSARRQAVQAAYREATRTPLETMDHCLAVMRHALAAVARGNPNAASDAVVGLLMASAGFEGALWNVAINLGSITDEAFRQETLEQVERMRAEREEVLQAFHSLVPDPVVRFLKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JJ3</accession>
    <name>Q67JJ3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Succinate dehydrogenase flavoprotein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3175</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3175"/>
    <dbReference type="EnsemblBacteria" id="BAD42157">
        <property type="protein sequence ID" value="BAD42157"/>
        <property type="gene ID" value="STH3175"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3175"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_7_1_9"/>
    <dbReference type="OMA" id="DPIPIQP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011280">
        <property type="entry name" value="Succ_DH/Fum_Rdt_flav_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF53">
        <property type="entry name" value="PTHR11632:SF53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01811">
        <property type="entry name" value="sdhA_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="9">
        <location>
            <begin position="5"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="10">
        <location>
            <begin position="466"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="593" mass="65572" checksum="7B0BECBCA885B040" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ1</accession>
    <name>TYSY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate synthase</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
            <ecNumber evidence="1">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyA</name>
        <name type="ordered locus">STH3117</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JQ1"/>
    <dbReference type="SMR" id="Q67JQ1"/>
    <dbReference type="STRING" id="292459.STH3117"/>
    <dbReference type="EnsemblBacteria" id="BAD42099">
        <property type="protein sequence ID" value="BAD42099"/>
        <property type="gene ID" value="STH3117"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3117"/>
    <dbReference type="eggNOG" id="COG0207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_0_9"/>
    <dbReference type="OMA" id="KQYLDLC"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141038" description="Thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="185"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <sequence length="283" mass="32760" checksum="6281432CE06D02AC" modified="2004-10-11" version="1">MNIEIQYLELCREILAENEQEIAEGRWTPNRTGMGTVGVFGRMLKHDFKEGFPALTTKKLFFNSVKAELLWFLAGRTDLEFLHERGCHIWDKDAARNGGKLGPIYGKQWRAWEGADGRTYDQIAWVIQQIREIAADPERPHPNSRRLVVSPWNVADLEEMALPPCHGPFQFHVKDGRLHLAVWQRSADLFLGVPFNIASYSLLLHMVAQVTGLAVGTYTHYLNDVHIYRNLLDQVRLQLTREPYPLPRLWLNPEVREIDQFTMDDIQLVDYKHHPPIRGELST</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ2</accession>
    <name>Q67JQ2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3 6">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3116</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3116"/>
    <dbReference type="EnsemblBacteria" id="BAD42098">
        <property type="protein sequence ID" value="BAD42098"/>
        <property type="gene ID" value="STH3116"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3116"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_5_2_9"/>
    <dbReference type="OMA" id="RDNQLPW"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="165" mass="18940" checksum="265CAC09ACA2995F" modified="2004-10-11" version="1">MGRNRAIGREGRLPWRLPAEMRHFRRTTMGKAVVMGRRTYESIGGPLKGRTNIVLTRDPGFRAPGCEVRHSVAELLQDRRPLYVIGGAEVYRQFLPHADEMILTRIDHDFEGDAFFPAWSEDEWELVGSTPHPRDEENPYDFVVEHYRRRRIPAGGAGQTEPEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JQ6</accession>
    <name>Q67JQ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein, histidine-and aspartate-rich</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH3112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD42094">
        <property type="protein sequence ID" value="BAD42094"/>
        <property type="gene ID" value="STH3112"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3112"/>
    <dbReference type="HOGENOM" id="CLU_1577703_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD42094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="169" mass="18985" checksum="4A1515DCD1886478" modified="2004-10-11" version="1">MSRAGCRSYAGGREGGERMSWRDDHDRRSGLCADLEDDRDCGCGGHGWNGRHDQDGGDRWDGDHGHGHHDHRGDHDHHGHHDHHHNHHGGHDGHHHRHRRRQRRLIPIGSVTVDCTPVTFRTPGQAINVVSCPVSWIIPGTRCIATIHTPPVQTPVLPIHRHRKACGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K24</accession>
    <name>Q67K24_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Flagellar motor switch protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fliY</name>
        <name evidence="9" type="ordered locus">STH2993</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FliN/MopA/SpaO family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197108.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2993"/>
    <dbReference type="EnsemblBacteria" id="BAD41976">
        <property type="protein sequence ID" value="BAD41976"/>
        <property type="gene ID" value="STH2993"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2993"/>
    <dbReference type="eggNOG" id="COG1886">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097058_3_1_9"/>
    <dbReference type="OrthoDB" id="1875967at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012826">
        <property type="entry name" value="FliN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001172">
        <property type="entry name" value="FliN_T3SS_HrcQb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00956">
        <property type="entry name" value="FLGMOTORFLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02480">
        <property type="entry name" value="fliN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="8">
        <location>
            <begin position="49"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="126" mass="13278" checksum="559D6CA98A1BEA7A" modified="2004-10-11" version="1">MAKHGLTQEEIAALFAQARQGEQKPEAAAFPDLNPEGSAQGDPSPLGLLADVELELTVELGQSRRSLREILAMGPGSVLELDKHAGEAVDLLVNGQLVARGEVVVIGENYGIRITELINPEGSAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K54</accession>
    <name>Q67K54_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41944">
        <property type="protein sequence ID" value="BAD41944"/>
        <property type="gene ID" value="STH2961"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2961"/>
    <dbReference type="HOGENOM" id="CLU_3104665_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="51" mass="5617" checksum="ED6437C01E13A2F4" modified="2004-10-11" version="1">MSDQPQGRTGERCKVAGTYSCTAGVKQYYNEGDAFGPCPQTGEETRWVRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K60</accession>
    <name>Q67K60_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197070.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2955"/>
    <dbReference type="EnsemblBacteria" id="BAD41938">
        <property type="protein sequence ID" value="BAD41938"/>
        <property type="gene ID" value="STH2955"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2955"/>
    <dbReference type="HOGENOM" id="CLU_1795501_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="144" mass="15673" checksum="8F2A42770F7E16AC" modified="2004-10-11" version="1">MEGQELFAGGGEPVVYLPTEAGTAIAPDGRKLVFFSVPALDVMIKQVLAAQPREYTYRWGYHPGERLHVLLFGWPTGHGAGLAIPEGVGDAILNFMQGTTDVYITAAPVGDKLRGPVTPEVIDELRFGMTVYLPEVKFKPEGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KE0</accession>
    <name>Q67KE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative cadmium-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196992.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2875"/>
    <dbReference type="EnsemblBacteria" id="BAD41858">
        <property type="protein sequence ID" value="BAD41858"/>
        <property type="gene ID" value="STH2875"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2875"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_4_9"/>
    <dbReference type="OMA" id="QNIWFSI"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="608"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="656" mass="69112" checksum="234676A527380E62" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KM6</accession>
    <name>Q67KM6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endonuclease III</fullName>
            <ecNumber evidence="10">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nth</name>
        <name evidence="13" type="ordered locus">STH2787</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="10"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196906.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2787"/>
    <dbReference type="EnsemblBacteria" id="BAD41772">
        <property type="protein sequence ID" value="BAD41772"/>
        <property type="gene ID" value="STH2787"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2787"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_3_9"/>
    <dbReference type="OMA" id="QIIWYGR"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10576">
        <property type="entry name" value="EndIII_4Fe-2S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="12">
        <location>
            <begin position="40"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD41772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="235" mass="26254" checksum="A00B1E36EBAC7662" modified="2004-10-11" version="1">MARKSVDTEAILRKLEEMYPDAKCALNHRNAFELLVATVLSAQCTDARVNIVTARIFPRYNRPEHFAALSVDEIGEMIRDCGLWKSKAKNIQGLSQMLLEKHGGEVPSTMEELIQLPGVGRKTANVVLSNAFGIPAIAVDTHVFRVANRLGLAEAKTPEETERQLMERIPREYWSQAHHWLIYHGRQVCHARNPQCSQCPLLPHCRFGRQAGKGSVQRARVSSGRAARRAGGPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KR9</accession>
    <name>Q67KR9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2743</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196862.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2743"/>
    <dbReference type="EnsemblBacteria" id="BAD41728">
        <property type="protein sequence ID" value="BAD41728"/>
        <property type="gene ID" value="STH2743"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2743"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_9"/>
    <dbReference type="OMA" id="QADWMTL"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="80"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="30724" checksum="682164404DB1D2A0" modified="2004-10-11" version="1">MSESGGVLVAWRRFRANRRAMLGLYVLFALILASACAPLFTHHDPDLPDIRQAEQPPSREHWLGTDELGRDVYARLLYGGRVSLTVGLVAVSIYMAIGVVLGAVAGYYGGAVDSAIMRLTDVVMIVPFFPLALTMAAALRPSVYNTMLVIGLLGWTGVCRLVRGEFLTLRSREYVEAARAIGARDGRIIFRHILPNALGPILVAATLGVGSAILSEAGLSFLGFGVQQPTPSWGNMLAAALSLKVLLREPWIWLPPGLLIFVAVLAVNFVGEGLREALDPRLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KU0</accession>
    <name>Q67KU0_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6431 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41706">
        <property type="protein sequence ID" value="BAD41706"/>
        <property type="gene ID" value="STH2721"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2721"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1320339_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045536">
        <property type="entry name" value="DUF6431"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20020">
        <property type="entry name" value="DUF6431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6431" evidence="1">
        <location>
            <begin position="29"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="204" mass="23737" checksum="C49243DCA89C0C0A" modified="2004-10-11" version="1">MCILLPLGQNVQTYLRKYGTCGPDLPLHCPGCGGRMRRHGRYWRWVFTAHQKAYIPIYRWWCPGCRKTCAILPDFLKPYARFITLVREAVVRGRVRRGLPWSTLARRLSSPTVSWLSEKTLRRWLVRARALAGEWSQYLAERVLRFWPDTDLDALTPRREGPDATLHFLLDVGDWYRRQMGRRPEEHGGVFAALNRLGEGTASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KY6</accession>
    <name>Q67KY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Endo-1,4-beta-glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196797.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41660">
        <property type="protein sequence ID" value="BAD41660"/>
        <property type="gene ID" value="STH2675"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2675"/>
    <dbReference type="eggNOG" id="COG1363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_0_2_9"/>
    <dbReference type="OMA" id="FATRYIH"/>
    <dbReference type="OrthoDB" id="900854at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="366" mass="39857" checksum="1BA24D84E3E9706C" modified="2004-10-11" version="1">MEREAQLVDQRLKMYEELTSANGISGFEEPVRQVMRKYLEPLCDEIISDNLGSIAGIKKGQAGGPRIMLAGHLDEVGWMVTQITDKGFLKIQPVGGWWSQVMLAQRVKVLTRKGELIGVVGSKPPHILTQEERNKVVQIKDMFIDIGASSKEEAQEWGVRPGDPIVPYCDFHVMPNPKILMNKAWDNRAGCAVAIMVLEELKGQDHPNEVYAVGTVQEEVGLRGATTMANTIEPDIAFALDVGLATDVPGGDGQVEGKMGAGPLIMIYDASMVPHRGLRDLVIDTAEELEIPIQFQAMAGGGTDAGRIHIQGRGVPSLAVGFATRYIHSHAALIHRDDMENAAKLLAAVIKKLDAETVARLKNNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L27</accession>
    <name>Q67L27_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2634</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2634"/>
    <dbReference type="EnsemblBacteria" id="BAD41619">
        <property type="protein sequence ID" value="BAD41619"/>
        <property type="gene ID" value="STH2634"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2634"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512582_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13492">
        <property type="entry name" value="GAF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="430"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="536" mass="59343" checksum="E579CD394EE57C82" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L44</accession>
    <name>Q67L44_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminoglycoside 6'-N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196739.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2617"/>
    <dbReference type="EnsemblBacteria" id="BAD41602">
        <property type="protein sequence ID" value="BAD41602"/>
        <property type="gene ID" value="STH2617"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2617"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_12_2_9"/>
    <dbReference type="OMA" id="NHLIMIK"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019432">
        <property type="entry name" value="Acyltransferase_MbtK/IucB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM01006">
        <property type="entry name" value="AlcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="192" mass="21997" checksum="AA12ADA270432177" modified="2004-10-11" version="1">MQQEIRFRRLTEDDLDRMHRWLNTPHVMRWYSPGGRTRAQVEQEFGPYIRREKPTEPYLILYGNTPIGYIQTYRINHWPDYARHIGVDDDTAGVDVIIGEPEFVGRGLGPAAIRAFMRQVVFGSFGARSCVIGPEEGNLAAIRAYEKAGFRFWKRADVPGEPAPEYLMRMTPEDLQADDVHAAARPDANVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD4</accession>
    <name>Q67LD4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196650.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2527"/>
    <dbReference type="EnsemblBacteria" id="BAD41512">
        <property type="protein sequence ID" value="BAD41512"/>
        <property type="gene ID" value="STH2527"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2527"/>
    <dbReference type="HOGENOM" id="CLU_727454_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="40985" checksum="6C425CB49F551143" modified="2004-10-11" version="1">MSRYADITELIAEQAADPTEQKALEAVARKVARELERPVPYRPEFRDALREELMRTARRRLRPWYRRPAVVGPGLAAVAATVALAVGLQLAQPPAGQGPGQVAQHEPAPSVAPPLSTDPAPPGAQGGADTPYLVALPADLPEVRLADERTTEESLLAPAASPVIASVQLMRLTARPGEAEFRTMAARLGFRGESRRTDRGWVVADEDRTLTMTMDGTVQYADLTEPDADAPRVDEQAAGQAAQRFLDQAALPVHSQPDITAREDGFMVVYTEHVEGRPVVNARTEIAVNQAGTVVRAKAYVPSGVTIHATYTEFVSERQAVEMAESRGGSFRRAELVWVRSVGDGTVYLQPAWRVLGTNAQGTPVARYVAALTQQDGAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LD6</accession>
    <name>Q67LD6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2525"/>
    <dbReference type="REBASE" id="10274">
        <property type="entry name" value="M.StpORF2525P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41510">
        <property type="protein sequence ID" value="BAD41510"/>
        <property type="gene ID" value="STH2525"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2525"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_1_9"/>
    <dbReference type="OMA" id="MIEMWDD"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="8">
        <location>
            <begin position="65"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="304" mass="33627" checksum="BCE07E060437151B" modified="2004-10-11" version="1">MQCAPMIRRSFAPRREIIPGRTIADVRIRSPRPAGRRMTGGRCMFANRIYNADARNMSFIPDGVVDLVVTSPPYNVGKNYATHDDCLSMEEYLDLLEQVWRECYRVLAPGGRIAINVAGVDRKPYLPLHAYITLQMIRLGFQMRGEIIWNKGASVGVSTAWGSWCSPSNPTLRDLHEYILVFSKEDWRMGHRGETDLTPEEFVTYTKSIWEFPTVSAKKVGHPAPFPLELPSRLIKLYTYKGDLVLDPFNGSGTTCQAAALLGRRWIGVDIDPGYCALAEKNMRTLVDGAGGAGLGAASGVAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK7</accession>
    <name>Q67LK7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spore germination protein GerKC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gerXC</name>
        <name evidence="4" type="ordered locus">STH2454</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKC lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196577.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2454"/>
    <dbReference type="EnsemblBacteria" id="BAD41439">
        <property type="protein sequence ID" value="BAD41439"/>
        <property type="gene ID" value="STH2454"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2454"/>
    <dbReference type="eggNOG" id="ENOG502Z849">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051140_0_2_9"/>
    <dbReference type="OMA" id="IDCTFRI"/>
    <dbReference type="OrthoDB" id="1421701at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008844">
        <property type="entry name" value="Spore_GerAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038501">
        <property type="entry name" value="Spore_GerAC_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35789">
        <property type="entry name" value="PTHR35789"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05504">
        <property type="entry name" value="Spore_GerAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02887">
        <property type="entry name" value="spore_ger_x_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0309">Germination</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="412" mass="45523" checksum="F6B0670A992C4437" modified="2004-10-11" version="1">MPRSLLVLLLVLPLLAGCWNKMEIEEGAYVLALGVDEGRGGSLAITVVIAKPRALAGKEGGSPEEPPVLITTVEAPGIAAATNILHGYIGRRVQFHHVQAVFVHEQLAREKGLVFLDEVARFRQLRETAFLIVTREPAAEFLQRVAPELDINPIKFIEQLTYHTRTSGTLPSASQISSFIALLNAEYQEPIAYYAALSAEESGSETISPERESQIEAGVLPRSGGPAVEMIGAAVFRGRRMVGVLNGEEVRALLVLQNRFQGAFDAIPDPGDPDEFIILHVSRGRPTRIFVDRLGEKPSIRAYITLEAEVVGVPSGIDYALPDRQDELEQAIGQHVLQTMERLIEKTQRWESDVAGFGRHAVSAFPTVQTWEAYNWPRRYAEAEITATVDVRLRRFGLTLAPTRSSEERMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL7</accession>
    <name>Q67LL7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2444"/>
    <dbReference type="EnsemblBacteria" id="BAD41429">
        <property type="protein sequence ID" value="BAD41429"/>
        <property type="gene ID" value="STH2444"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2444"/>
    <dbReference type="eggNOG" id="COG3294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081221_0_0_9"/>
    <dbReference type="OMA" id="ICPVMEY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="57"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="241" mass="26810" checksum="D8EAC0ABBAFC7CF4" modified="2004-10-11" version="1">MFRRRQRGSRQVADVTGGPGLGKVTFEQVRQDPEVRAYIVKANEMLAAIGFTEHGLRHVGLVADRAMGLMAELDRPPRLRELAGIAALLHDIGNIVNRQGHGQTSALLAHHILSRMGMAPEEIAIIMSAIGNHEEEIGNPVHDVSAALILADKSDVHRTRVYNRDVATFDIHDRVNCAVTHSELHAFPAEKVIRLSLTVDTSIIAIMEYFEIFLSRMIMCRRAAAHLGCQFQLVMNENRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LN8</accession>
    <name>Q67LN8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2423</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S51 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196546.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2423"/>
    <dbReference type="EnsemblBacteria" id="BAD41408">
        <property type="protein sequence ID" value="BAD41408"/>
        <property type="gene ID" value="STH2423"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2423"/>
    <dbReference type="eggNOG" id="COG3340">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067063_0_0_9"/>
    <dbReference type="OMA" id="LFPMPNV"/>
    <dbReference type="OrthoDB" id="1485646at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03146">
        <property type="entry name" value="GAT1_Peptidase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005320">
        <property type="entry name" value="Peptidase_S51"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03575">
        <property type="entry name" value="Peptidase_S51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="26755" checksum="804EB5CD27A2B582" modified="2004-10-11" version="1">MKQIVAMGGGGFSMEPENPLLDRYVLGLTGKARPKVCFLPTASGDAEGYIVNFYRAMTQLGAEPTHLSLLRPTVRDMAALLLVQDVIYVGGGNTKNMLALWREWELDQVLHTAWEQGIILAGLSAGSICWFEQGVTDSIPGELTALPGLGFLKGSNCPHYDGEPERRPAYHRLRKAGVLSDGIAADDGVALHYVGEHLFKVVSSRPGARAYWVYAVNGEVREEPIDPVYLGESEPLTTTGRGEDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LZ9</accession>
    <name>Q67LZ9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative PTS system, IIA component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2312"/>
    <dbReference type="EnsemblBacteria" id="BAD41297">
        <property type="protein sequence ID" value="BAD41297"/>
        <property type="gene ID" value="STH2312"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2312"/>
    <dbReference type="eggNOG" id="COG1762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013442_1_1_9"/>
    <dbReference type="OMA" id="CNSSCKY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00211">
        <property type="entry name" value="PTS_IIA_fru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013011">
        <property type="entry name" value="PTS_EIIB_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51099">
        <property type="entry name" value="PTS_EIIB_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRD" evidence="4">
        <location>
            <begin position="267"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-2" evidence="3">
        <location>
            <begin position="379"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-2" evidence="2">
        <location>
            <begin position="478"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="634" mass="69029" checksum="E8C958CB957C11AE" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M42</accession>
    <name>Q67M42_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41253">
        <property type="protein sequence ID" value="BAD41253"/>
        <property type="gene ID" value="STH2268"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2268"/>
    <dbReference type="HOGENOM" id="CLU_1554519_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269900" evidence="1">
        <location>
            <begin position="25"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="172" mass="19491" checksum="FC719EBCC910B2A9" modified="2004-10-11" version="1">MSSMRRTILLLVVSALLVGCSARANLTQLPDAVTFTPITEATDFNIDAELVLQPHPELPNSWQGSMTFRFVSLVDEDLHNVRVAIFYPEQMVEMLLIPEETLVLPPSGRRFDVTPERPEWRFTHTVAAFDWEQLGEVKAAILNPIRLRIVWDGAERFLEIPPSEIAVREPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M64</accession>
    <name>Q67M64_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2246</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2246"/>
    <dbReference type="EnsemblBacteria" id="BAD41231">
        <property type="protein sequence ID" value="BAD41231"/>
        <property type="gene ID" value="STH2246"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2246"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1721434_0_0_9"/>
    <dbReference type="OMA" id="WIDGAPC"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="152" mass="17769" checksum="BCF23AB26ACB6004" modified="2004-10-11" version="1">MRSRTSTSRRRIPQVTTFRIALEELLRKTGVDDFDFLREGLRVLAQGLMELEVSQRIGADRYERSAERSTYRNGYRERQWDTRVGTIDLQIPKLRKGSYMPSFLEPRRRAEKALVAVVLEAYVNGVSTRKVDDLAQVSREQLDIRGVEKVHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M80</accession>
    <name>Q67M80_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2228"/>
    <dbReference type="EnsemblBacteria" id="BAD41213">
        <property type="protein sequence ID" value="BAD41213"/>
        <property type="gene ID" value="STH2228"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2228"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_9"/>
    <dbReference type="OMA" id="VDMLHIP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="346" mass="36600" checksum="54715DDA60C16128" modified="2004-10-11" version="1">MAGCGSSGTSTDATSPASTEQSATHGTPAEQTASQQTSTEPAGQEETKTWKPEKAVTLIVPYSAGGSTDVLARVVEKIWPKYVDQPLTVVNQPGGSGWVGREAVAKAAPDGYTLMIGYGSGEDLIAPQLREAPFDPINDFQPIALLSNHAILFATSTESGYETMEQLLEAAKSAEVTAALSGVGNLMDITVRAVAKAAGGEITGVPFAGGGPATTAMVGNQVDFGPGHQSELMPHVKGGRLRILAVASPERESMLADVPTLKELGIDYVNAGSLKGIAAPAGLPEEILAYYEDLFAKITSDPEFQQAMADVYQPVNYKNPEEFGAMIREYYEMYSRLIKELDIHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M86</accession>
    <name>Q67M86_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2222</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196347.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2222"/>
    <dbReference type="EnsemblBacteria" id="BAD41207">
        <property type="protein sequence ID" value="BAD41207"/>
        <property type="gene ID" value="STH2222"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2222"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037802_0_1_9"/>
    <dbReference type="OMA" id="VISWFRI"/>
    <dbReference type="OrthoDB" id="1518604at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="19"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="19840" checksum="0001D6E8E3E6DFF5" modified="2004-10-11" version="1">MELLLGLLIGTAFGFILRRSRVASQACIRGALALMDFHMLKLLLTAVGVTLILVFPLSTLGIVNFSVKTTYVVGIVVGGLLFGIGMAVAGFCPGTMLAALPGGDRRFWWAVAGGLAGSFAYALLYEPLQPLLIAPLNFGKLTLPELLGLNPVVTGVGAGFLLLAGVVLLDRLTRPKQAEIPDLAKLGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MC0</accession>
    <name>Q67MC0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2188"/>
    <dbReference type="EnsemblBacteria" id="BAD41173">
        <property type="protein sequence ID" value="BAD41173"/>
        <property type="gene ID" value="STH2188"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2188"/>
    <dbReference type="HOGENOM" id="CLU_1824343_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="141" mass="14687" checksum="1A44A413D7EB07EF" modified="2004-10-11" version="1">MGNTTGAGVSGMDRTEELLRASFSRLEGLIGEVRDLRYKIKSVQGDVESLRAALGGGGNGNAEPSGSAEGEPSAAARSDGTGSEPGESGSAAAGAKAGPGDSAFEERLRRIEQRLDYLASKWLELDEKLFRLQKRLGSTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF6</accession>
    <name>Q67MF6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">4Fe-4S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH2152</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196277.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2152"/>
    <dbReference type="EnsemblBacteria" id="BAD41137">
        <property type="protein sequence ID" value="BAD41137"/>
        <property type="gene ID" value="STH2152"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2152"/>
    <dbReference type="eggNOG" id="COG1600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030790_2_0_9"/>
    <dbReference type="OMA" id="ICDTDLS"/>
    <dbReference type="OrthoDB" id="1478472at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004453">
        <property type="entry name" value="QueG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013542">
        <property type="entry name" value="QueG_DUF1730"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30002">
        <property type="entry name" value="PTHR30002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08331">
        <property type="entry name" value="DUF1730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00276">
        <property type="entry name" value="TIGR00276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="181"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD41137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="387" mass="42842" checksum="880F341FB010B0BC" modified="2004-10-11" version="1">MPDLQRLKAEMKDRAREIGIDLVGVAPAEPFLRERQILEERIARGRGPNPYEHPVVEERVYPERLLPGVKAIIACGIAYYHPDAPRPPEDRVALRGWLSRYCRGRDYHQILKDRILALAAWLERRAPGHRHVAYVDTGPPLDRAVAERAGIGKFGKHTNLITREYGTWVFLGELLTTLPLPPDEPQPPACGACTLCIDACPTGALSEWRLEWDRCLSHITQMKGIIPAEYREVMGNRLFGCDDCQDVCPYNRRARPSRHPEFAPSPDLDGGEPDLLRLMAMTKGDFRRWFEPTAAGWRGKTTIQRNALIALGNSGDPAALPALTAALESESAILRAHAAWALGRLGRLAPDARDAARSALARRATCETDPAVRLELGAALESLGGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MF9</accession>
    <name>Q67MF9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ArsR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196274.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2149"/>
    <dbReference type="EnsemblBacteria" id="BAD41134">
        <property type="protein sequence ID" value="BAD41134"/>
        <property type="gene ID" value="STH2149"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2149"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_0_3_9"/>
    <dbReference type="OMA" id="AWIDRYR"/>
    <dbReference type="OrthoDB" id="1617768at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="129" mass="14995" checksum="A598CD45E868305D" modified="2004-10-11" version="1">MGVRRMERVLQALADPTRRRILDLLAQSDLAAGEIARHFPDISRPAVSQHLAVLREAGLVRERREGRRRIYRLEPEPLRELWEGWLSHYQRFWDDRLAGLKRLVESEDWRGEGEQTDGGSGIPTDRRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MH0</accession>
    <name>Q67MH0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferrous ion uptake protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">feoA</name>
        <name evidence="3" type="ordered locus">STH2138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196263.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2138"/>
    <dbReference type="EnsemblBacteria" id="BAD41123">
        <property type="protein sequence ID" value="BAD41123"/>
        <property type="gene ID" value="STH2138"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2138"/>
    <dbReference type="eggNOG" id="COG1918">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186691_0_0_9"/>
    <dbReference type="OMA" id="QQIAIGR"/>
    <dbReference type="OrthoDB" id="1934041at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="7887" checksum="84AB43AB40BFC83B" modified="2004-10-11" version="1">MTLDQTKPGQTFLIRSIDDPAARLAAIRFGIAEGSRASCQTVLPGGPVVVRKGKQEMALGRSLAHRITVELVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MY5</accession>
    <name>Q67MY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1973"/>
    <dbReference type="EnsemblBacteria" id="BAD40958">
        <property type="protein sequence ID" value="BAD40958"/>
        <property type="gene ID" value="STH1973"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1973"/>
    <dbReference type="eggNOG" id="COG2153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056607_6_4_9"/>
    <dbReference type="OMA" id="PHVEMRK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="PTHR13355"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="147" mass="16306" checksum="74C6BF8F5ED4515A" modified="2004-10-11" version="1">MAIRTVRVTTPEQLEAAFAIRRKVFIEEQNVPPEEELDGLDHTAAHVLALDPAGRPVATGRIIPYGEGTGKLQRIAVLPEFRGQGYGRAVVGALEEMGRAMGFRRFVLGAQTHAEPFYRRLGYRTTSPEVFLDAGIPHVHMEKVLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N07</accession>
    <name>Q67N07_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Type IV prepilin-like leader peptide processing enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196078.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1951"/>
    <dbReference type="MEROPS" id="A24.019"/>
    <dbReference type="EnsemblBacteria" id="BAD40936">
        <property type="protein sequence ID" value="BAD40936"/>
        <property type="gene ID" value="STH1951"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1951"/>
    <dbReference type="eggNOG" id="COG1989">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1288347_0_0_9"/>
    <dbReference type="OMA" id="TEYNIFN"/>
    <dbReference type="OrthoDB" id="2046608at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_A24" evidence="3">
        <location>
            <begin position="69"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="214" mass="22700" checksum="CF5B95CE78AF711E" modified="2004-10-11" version="1">MSGQAWPAALLGVALGAALGYLGVRFSPRWLTRPAPRWLSASLVAGIALAAGLLGYFHRLTPYFWHQLALVAILLAAAFIDLQDHIIPNDLVLVGFAVWLPAMLLFPYDGKTWLSALGGGAAAFAFFYLLAVLVPDGMGMGDVKLALVMGLFLGAGWVAMALILAFLLGGLASAVLLATRRVGRRGHIPFGPFLAAGGLITILWGSQIWNWYAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N21</accession>
    <name>Q67N21_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1937</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196064.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1937"/>
    <dbReference type="EnsemblBacteria" id="BAD40922">
        <property type="protein sequence ID" value="BAD40922"/>
        <property type="gene ID" value="STH1937"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1937"/>
    <dbReference type="eggNOG" id="COG1820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_2_1_9"/>
    <dbReference type="OMA" id="HIHGRAG"/>
    <dbReference type="OrthoDB" id="1026967at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="PTHR11113:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="8">
        <location>
            <begin position="52"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="309"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="401" mass="42148" checksum="037239858E67C08B" modified="2004-10-11" version="1">MMLFTCSRIHAEGGLVDGYVLVDGRRIAAVEAGPVPAELQGVDRVDLPDCDLIPGMIDLHIHGAGGWPVEGADVGLLQGLGRFLAGFGVTGFLPSASARPLEELEEVARQVRAATEAEYDGAAILGLHLEGPFLNPKRPGAMHIHNLRTPSVAEAERLLAAGGGTVRRVSLAPELPGAPELIRYLVAQGVTVAGAHTDATYAETVAGIEAGVSLATHTYNAMRGLHHREPGAVGAYLTDDRVTCEIICDLLHVHPAAVRIALRAAGPDRVALISDAIPAAGLKPGHYLLWGRDLYIDEQGYSKLADGTIAGSTKLMLHGLRNLVEVLGVPWAEAVRMASLIPARAIGLADRKGSLAPGKDADLVAIGPGWQVAWCVVEGRVVRRPGDPPPAYNPEAKAIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N32</accession>
    <name>Q67N32_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="7 19">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cobS</name>
        <name evidence="20" type="ordered locus">STH1926</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="7 19"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196053.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1926"/>
    <dbReference type="EnsemblBacteria" id="BAD40911">
        <property type="protein sequence ID" value="BAD40911"/>
        <property type="gene ID" value="STH1926"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1926"/>
    <dbReference type="eggNOG" id="COG0368">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_3_1_9"/>
    <dbReference type="OMA" id="FMDSMDG"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="PTHR34148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="BAD40911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="270" mass="27339" checksum="A8F4EE8A557AD0E7" modified="2004-10-11" version="1">MRIALSFLTRIPMGRLETPDYMRDLGRAAGFFPLVGLLVGAICLIAWLAGRLLFGPAVAGVAAVAAGLGATGAVHLDGLMDAADGLLSARSRERMLEIMKDSRVGVMGAAAGALALMLRWALLLELTPPRAAAALLVAPAVGRMVIPLAAVRWPPARAEGMGAAFGRHVGGAQLAGALLGGLTLAVGVPGLLAWWGQSPAAPPGLTAAVTVSALRGLGAWLAALGVGLAGGRWLSRRLGGLTGDTYGALCELAELAAIACFALQTGRWTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N55</accession>
    <name>Q67N55_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1903"/>
    <dbReference type="EnsemblBacteria" id="BAD40888">
        <property type="protein sequence ID" value="BAD40888"/>
        <property type="gene ID" value="STH1903"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1903"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057821_0_0_9"/>
    <dbReference type="OMA" id="PHAGQTR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="153"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="312" mass="34897" checksum="49C234B9D4202EFA" modified="2004-10-11" version="1">MQLTGFHHLTAMTAHIRENRRFYTEVLGLRLAKRTVNQDDVRAYHLYYTDGAGSPGTDITFFDWDIPPERRGTRSIVRTMFRVAGAEALSWWQRRLADAGLAVGEVMDWAGRPTLPFEDPEGQRLALVDDGGQGPDPVIWGRSPVPPEYQLRGLGPVVLSVPDPGPTAAVLIQVLGMRRVRDLPSPEDGRHVLHVFAFGEGGPAAELHVLAQPDLPAAQAGAGGVHHVAFRTPNEREYQAWLRRLNQLGVRNSGPVDRFWFRSLYFREPGGILFEIATDGPGFAIDEPPESWARSWRFRPSWSRGGRRSRPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N61</accession>
    <name>Q67N61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative phosphoserine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1897"/>
    <dbReference type="EnsemblBacteria" id="BAD40882">
        <property type="protein sequence ID" value="BAD40882"/>
        <property type="gene ID" value="STH1897"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1897"/>
    <dbReference type="eggNOG" id="COG4359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058495_2_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="225" mass="25009" checksum="C86034D3B8B2255B" modified="2004-10-11" version="1">MLLTDFDFTISLVDVGDLICGTLAPYPADVLARYARGEAGSRDLWLASFAHVDRDEAVALADRVDIDPGFRDLVAWAEREGIPLAVVSDGFTLYIEHILGREGLGHLPVFANRYVERGRLEWPNGNPACPLCGCCKAAVARRLKASGSRVIYFGDGSSDVYGASFADWVFAKSTLARFLEQNRSPYFPFTGFADALDVLRQNLDRFRDGTMQPRSTLRPHPRCRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N85</accession>
    <name>TDH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-threonine 3-dehydrogenase</fullName>
            <shortName evidence="1">TDH</shortName>
            <ecNumber evidence="1">1.1.1.103</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tdh</name>
        <name type="ordered locus">STH1873</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-threonine + NAD(+) = (2S)-2-amino-3-oxobutanoate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:13161"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78948"/>
            <dbReference type="EC" id="1.1.1.103"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.103" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N85"/>
    <dbReference type="SMR" id="Q67N85"/>
    <dbReference type="STRING" id="292459.STH1873"/>
    <dbReference type="EnsemblBacteria" id="BAD40858">
        <property type="protein sequence ID" value="BAD40858"/>
        <property type="gene ID" value="STH1873"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1873"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_0_9"/>
    <dbReference type="OMA" id="VCEMSGH"/>
    <dbReference type="UniPathway" id="UPA00046">
        <property type="reaction ID" value="UER00505"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008743">
        <property type="term" value="F:L-threonine 3-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019518">
        <property type="term" value="P:L-threonine catabolic process to glycine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00627">
        <property type="entry name" value="Thr_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004627">
        <property type="entry name" value="L-Threonine_3-DHase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00692">
        <property type="entry name" value="tdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000160861" description="L-threonine 3-dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="266"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atom" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00627"/>
        </source>
    </evidence>
    <sequence length="351" mass="38050" checksum="2CB65F2279AB6279" modified="2004-10-11" version="1">MAETMRALRKLEAGPGATLQEVPIPTIGPRDVLVKVRAASICGTDYHIYTWDPWSAGRVKPPLTIGHELAGEVVAVGREVTACKVGDYVSAETHIVCNRCPRCHMGEYHLCENTKILGVDTDGAFAEYVAVPEQNIWVNDKDIPFELQSIQEPLGNAVHTALNGDLTARSVLITGCGPIGIMSVPVAKMAGAEIVMAMDINEYRLQLAGQLGADVLINPTKQDPVEVVRSYTRGYGADVVLEMSGNPTAIRQGLKAARNGARISLLGLPGRPLELDLAADVIMRGLVLQGITGRRMWQTWYQVRSLYRAGLAERLRPLVTHRMPLEQIDAAMELMGSGQSGKIVLVPDLKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NF6</accession>
    <name>SCPB_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Segregation and condensation protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">scpB</name>
        <name type="ordered locus">STH1802</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Homodimerization may be required to stabilize the binding of ScpA to the Smc head domains. Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Associated with two foci at the outer edges of the nucleoid region in young cells, and at four foci within both cell halves in older cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ScpB family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195930.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NF6"/>
    <dbReference type="SMR" id="Q67NF6"/>
    <dbReference type="STRING" id="292459.STH1802"/>
    <dbReference type="PRIDE" id="Q67NF6"/>
    <dbReference type="EnsemblBacteria" id="BAD40787">
        <property type="protein sequence ID" value="BAD40787"/>
        <property type="gene ID" value="STH1802"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1802"/>
    <dbReference type="eggNOG" id="COG1386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045647_5_2_9"/>
    <dbReference type="OMA" id="DGWRFYT"/>
    <dbReference type="OrthoDB" id="1771906at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01804">
        <property type="entry name" value="ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00281">
        <property type="entry name" value="TIGR00281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0159">Chromosome partition</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000273316" description="Segregation and condensation protein B">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="219" mass="23850" checksum="1A53B420DFBB727C" modified="2004-10-11" version="1">MIWNHGKMILEALLLASPEPLTIKRIAEVIGLDERDAALLMADLQKDYEAPHRGLAIREMAGGYVLTTRPEAAEYVERLLQPKSRGLSHAALETLAIIAYRQPITKAEIENVRGVKVDRALETLLERGLIEDKGRKEAPGRPILYGTTAEFLRYFGLRELSELPPVEIDTSEPGLIVPTRTGGGEAEREVQASLFAGGEEPSAEAADGGAGESTHGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ2</accession>
    <name>Q67NJ2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1766"/>
    <dbReference type="EnsemblBacteria" id="BAD40751">
        <property type="protein sequence ID" value="BAD40751"/>
        <property type="gene ID" value="STH1766"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1766"/>
    <dbReference type="HOGENOM" id="CLU_2095635_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="116" mass="12676" checksum="0986D79CA2FC4ACF" modified="2004-10-11" version="1">MDRVREGDARNSPVFGKPAHRSPSRKPMIGYAISAEIATPDLNLLCVSGADRPSGRPDPSARGFRHRVPARNRPRSWTWLLRRASRPDVGRESAAAPAPPLACRSCCPAYAHLLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NJ6</accession>
    <name>Q67NJ6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1762"/>
    <dbReference type="EnsemblBacteria" id="BAD40747">
        <property type="protein sequence ID" value="BAD40747"/>
        <property type="gene ID" value="STH1762"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1762"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_9"/>
    <dbReference type="OMA" id="CVVTSMA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="11"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="306" mass="32925" checksum="6BDB478C01D11929" modified="2004-10-11" version="1">MCAVAEDRHWDIVIVGAGMAGLATAIWARRLGLEPLVLEAAPRPGGQLNQIRGRIFDYPGLDLPSGEALTERLMRQAAEAGARVDLGRTVRAVSAAERTCLTSEGPVTGRALVIATGLRARRLGVEGERALYEGNWVRRPSHDLPWFRGRRVAVIGGGDRALENAGLLLPVAREVHLLSRGPLRARTDLSAPVLANDRLVHHERARVGRFDVRPDGVTVEVHQADRVWPLEVDAVCIYIGNEPNSQLVAGQVELDPEGYIVTDRFGQTSVPGVFAAGDVCTPPAYQSLANAAGQAMVVAKRIALSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM4</accession>
    <name>Q67NM4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195862.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1734"/>
    <dbReference type="EnsemblBacteria" id="BAD40719">
        <property type="protein sequence ID" value="BAD40719"/>
        <property type="gene ID" value="STH1734"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1734"/>
    <dbReference type="HOGENOM" id="CLU_2221883_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="106" mass="12006" checksum="81B6B473ACACCEC9" modified="2004-10-11" version="1">MGVVVQSFDGRMPGDLYERLVRLRRRHRTVLQAAAAGDFSNEAELHALSRRLGRMLAETARMPVEQRGGLRMLEISLAVDRMFPELRAWNQQAAFRYPPAGRAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NN5</accession>
    <name>Q67NN5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dmu-9_3-mt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1723"/>
    <dbReference type="EnsemblBacteria" id="BAD40708">
        <property type="protein sequence ID" value="BAD40708"/>
        <property type="gene ID" value="STH1723"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1723"/>
    <dbReference type="eggNOG" id="COG2764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_17_3_9"/>
    <dbReference type="OMA" id="LEKQMWG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06588">
        <property type="entry name" value="PhnB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028973">
        <property type="entry name" value="PhnB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06983">
        <property type="entry name" value="3-dmu-9_3-mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dmu-9_3-mt" evidence="1">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="139" mass="15245" checksum="E904CA7219BFDD9C" modified="2004-10-11" version="1">MRIVPYLVFGGNAREAIQFYADVLDGRIEGILTFGDNPFPGMPEEAENRVMHAQLTVGDSALFFSDTYPGQDHRPGNQVAVTLLVDTVEEAERIYTALAEGAQEIALPLQQTDWSPAYAQVTDKFGVPFQINTVPADQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY5</accession>
    <name>Q67NY5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1623</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195752.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1623"/>
    <dbReference type="EnsemblBacteria" id="BAD40608">
        <property type="protein sequence ID" value="BAD40608"/>
        <property type="gene ID" value="STH1623"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1623"/>
    <dbReference type="eggNOG" id="COG1111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_3_2_9"/>
    <dbReference type="OMA" id="CIQSPKC"/>
    <dbReference type="OrthoDB" id="35275at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="DUF1998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="64"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="268"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="758" mass="84162" checksum="0EFA8EFA819E8A27" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY7</accession>
    <name>Q67NY7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195750.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40606">
        <property type="protein sequence ID" value="BAD40606"/>
        <property type="gene ID" value="STH1621"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1621"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_0_0_9"/>
    <dbReference type="OMA" id="WRRVPAT"/>
    <dbReference type="OrthoDB" id="1701876at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269292" evidence="1">
        <location>
            <begin position="39"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="56"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="246" mass="27125" checksum="51AD951CFD40F9F3" modified="2004-10-11" version="1">MLFIDRAWLVRRAVVVATLCLALFTISFLRSPAFQPTAATAWQADRPLTRADSPDDRVALTFDVTWQKQELAKILDILDQAGVKATFFVGGTFLQLYPDMVKEIARRGHEVGTLGQQIRDLSGMPENEIVSNLLGSQSALAKILGGPVRYFRPPSGKAPPEVLRAAHQARLVTVTYALDGQDHLGLTADQIVRRVVRKAQRGDIIRLTASDFVTETAEALPRILEGLQRRGFKLVRISDLVPADDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P31</accession>
    <name>Q67P31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195706.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1577"/>
    <dbReference type="EnsemblBacteria" id="BAD40562">
        <property type="protein sequence ID" value="BAD40562"/>
        <property type="gene ID" value="STH1577"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1577"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_9"/>
    <dbReference type="OMA" id="AMEMIMT"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="256" mass="27185" checksum="48511A0A380C39BB" modified="2004-10-11" version="1">MEKVILTREGHVAWLALNNPDRHNALSRQVVRDLQEAAHQLAVDRSVRAVVVHGGVSKSFCSGADLKERQGMDEPAVLDTVHALRSAVNSVAQLPMPVIAAIHGMAFGGGFELALACDIRIAAEDAQMGLTEVGWGIIPGAGGCARLPALIGPARAKELIFTARRLAAGEALALGLVNRVVGREELLDAARALAEEIARQAPLAVRAAKRAIDAGPGLAGALAAEWEAYRSIVPTRDRLEGLRAFAERRPPEFRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD5</accession>
    <name>Q67PD5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Ribosome biogenesis GTPase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1473</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1473"/>
    <dbReference type="EnsemblBacteria" id="BAD40458">
        <property type="protein sequence ID" value="BAD40458"/>
        <property type="gene ID" value="STH1473"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1473"/>
    <dbReference type="eggNOG" id="COG1161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_1_0_9"/>
    <dbReference type="OMA" id="GVLWPKF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019991">
        <property type="entry name" value="GTP-bd_ribosome_bgen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03596">
        <property type="entry name" value="GTPase_YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="9"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="129"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006230-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="286" mass="31212" checksum="C732630C9D113922" modified="2004-10-11" version="1">MIQWFPGHMAKARRILQENAPLVDVVLEIVDARCPLASRNPDLMALVAHKPQVLILNKADLADPAATRLWVERLNEPGRPAVALDAVRGTGAREVLAAIRRAFAPVLRAWTAKGRKPRPARVMAVGIPNVGKSSAINRLVGARRAPVGDKPGVTRGKQWIRIGKDVELLDMPGILVPKFEDQRDGLLLAATGAIKDEVFDQREVANHLLHLIWESLGAAVAERYGLTRLDPEPVANLEAIGRFRGLLRAGEQVDLDAAAQLVLREFREGRLGRVTLQPPDPPPGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PD6</accession>
    <name>Q67PD6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Signal peptidase I</fullName>
            <ecNumber evidence="4 6">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1472</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S26 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195602.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1472"/>
    <dbReference type="EnsemblBacteria" id="BAD40457">
        <property type="protein sequence ID" value="BAD40457"/>
        <property type="gene ID" value="STH1472"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1472"/>
    <dbReference type="eggNOG" id="COG0681">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028723_5_1_9"/>
    <dbReference type="OMA" id="RAEFIFW"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S26" evidence="7">
        <location>
            <begin position="17"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="189" mass="20883" checksum="9AE83D4D52534269" modified="2004-10-11" version="1">MADVPDERPAVRRKSPLREVLETLVLALLFALIIRTFVVEVYQVSGSSMTNTLYDQERVLVNKFIYKLVRDPRPGDIIVFKYPRQPERDFIKRVVAVAGDTVEMRGGVVYVNGEPFNEAPTVRLSAGDFGPVVVPPDSVFVLGDNRSNSEDSRYFGEVPLSHIRGLAVARIWPLTEISALALPAAEGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PF3</accession>
    <name>Q67PF3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smc</name>
        <name evidence="6" type="ordered locus">STH1455</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195585.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1455"/>
    <dbReference type="EnsemblBacteria" id="BAD40440">
        <property type="protein sequence ID" value="BAD40440"/>
        <property type="gene ID" value="STH1455"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1455"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_9"/>
    <dbReference type="OMA" id="HNKIAME"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="5">
        <location>
            <begin position="525"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="836"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="248"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="994"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="836"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1193" mass="132127" checksum="044B4174F83F89CD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PH9</accession>
    <name>Q67PH9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1429"/>
    <dbReference type="EnsemblBacteria" id="BAD40414">
        <property type="protein sequence ID" value="BAD40414"/>
        <property type="gene ID" value="STH1429"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1429"/>
    <dbReference type="HOGENOM" id="CLU_2319087_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="10177" checksum="989BC54F57674DD4" modified="2004-10-11" version="1">MRPQALLLPLVSAAALLPAVAGLSQGPVVGMAAVFIAVIVIAIVIIVVIAAAVVFIGMRAGHRPGVPFFLPAFLPAVRPNRRRGRRFSPTSCTPAAGVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PJ0</accession>
    <name>Q67PJ0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prephenate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1418"/>
    <dbReference type="EnsemblBacteria" id="BAD40403">
        <property type="protein sequence ID" value="BAD40403"/>
        <property type="gene ID" value="STH1418"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1418"/>
    <dbReference type="eggNOG" id="COG0287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055968_0_0_9"/>
    <dbReference type="OMA" id="EDVRIDH"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="32844" checksum="09A06AC0AFA734EC" modified="2004-10-11" version="1">MSSGFGTVAIWGVGLIGGSIGMALRQRGLADEVIGIGRPATRAAAADASGPGGGAGAWEVPEAVRLGAVDRMVTDPAAALARADLVILAMPVQPMIDLAPRAAPLLRPGTVVTDVASVKEAVVAAWERHLPDGVAFVGGHPMFGRERSGVAWASADLIPGCRWVLTPGQRAVTVLKAGRSGAEVSPLELVWDLAAALGALPVVMSPAEHDRRVAGASHLPQLVATALAAAALDLDETQPGTLELAAGGFRDATRIADSPAALWHEIWANNRPALREAVAAFRGVLADLEAAVDAGDFGALAAVFERAHAARRRVGGRTGGPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PL7</accession>
    <name>MSCL_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large-conductance mechanosensitive channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mscL</name>
        <name type="ordered locus">STH1391</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MscL family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195521.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PL7"/>
    <dbReference type="STRING" id="292459.STH1391"/>
    <dbReference type="EnsemblBacteria" id="BAD40376">
        <property type="protein sequence ID" value="BAD40376"/>
        <property type="gene ID" value="STH1391"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1391"/>
    <dbReference type="eggNOG" id="COG1970">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095787_2_3_9"/>
    <dbReference type="OMA" id="AWIIFLM"/>
    <dbReference type="OrthoDB" id="1863650at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00115">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019823">
        <property type="entry name" value="Mechanosensitive_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001185">
        <property type="entry name" value="MS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037673">
        <property type="entry name" value="MSC/AndL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036019">
        <property type="entry name" value="MscL_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30266">
        <property type="entry name" value="PTHR30266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01741">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01264">
        <property type="entry name" value="MECHCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81330">
        <property type="entry name" value="SSF81330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00220">
        <property type="entry name" value="mscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01327">
        <property type="entry name" value="MSCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000238042" description="Large-conductance mechanosensitive channel">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00115"/>
        </source>
    </evidence>
    <sequence length="146" mass="15956" checksum="17B8449FE9D4E4F3" modified="2004-10-11" version="1">MLSEFKRFALRGNVLDLAVGVVIGAAFNQIVNSLVNDVIMPPLGFLVGKMDFSNLYLNLSLTPYASLAEAREAGAPVIAYGLFLNNLLNFLIVAFAVFLLVRQVNRWRPTPPPEPPKTKECPYCLSTVPVKATRCAHCTSDLTAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PN4</accession>
    <name>Q67PN4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195504.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1374"/>
    <dbReference type="EnsemblBacteria" id="BAD40359">
        <property type="protein sequence ID" value="BAD40359"/>
        <property type="gene ID" value="STH1374"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1374"/>
    <dbReference type="HOGENOM" id="CLU_1446970_0_0_9"/>
    <dbReference type="OrthoDB" id="1844826at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="187" mass="20476" checksum="EE29342833051514" modified="2004-10-11" version="1">MVFKLRTRNAEFETGTFIPWVASGDATVSDDVACSGFWTARLGPGTGYIQQDGVAYPNRSFEVQFRVGAVGGMATSPLVMTLDFYDQPQFGRLFQWEQQRERLGGAYLPEPEPQPQGRLLRRFTLTIPAADIPTVDGATPVGCLQLNVDAGLSPNNTRRFRLTFAKPTAGGADLIVDAIHVIERTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q61</accession>
    <name>Q67Q61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1197</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195328.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1197"/>
    <dbReference type="EnsemblBacteria" id="BAD40182">
        <property type="protein sequence ID" value="BAD40182"/>
        <property type="gene ID" value="STH1197"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1197"/>
    <dbReference type="eggNOG" id="COG4586">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="ERMRCEI"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="24"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="330" mass="36817" checksum="84EAEF8223E43AAF" modified="2004-10-11" version="1">MTIITVRGLRKVFRVHRKPESSWARLRAALRPQTVEVEAVRGIDLTVDRGEMLAFIGPNGAGKSTTIKMLTGILHPTAGQAEVLGLVPWRDRQRLAFRIGSVFGQRSQLWLHLPPVDTFNLLARIYELDWLAYLRRRAELVELFELGEIMHTPARKLSLGQRMRCEIAASLLHRPEILFLDEPTIGLDPVAKGAIRDLIRRANREERVTVFLTSHDAGDIEHLCKRVVIINRGQVILDGSVGALKRDYMPERQIDLKLAAPVADELSLPGVRVLKASAYGLKLAVDTRVSPIEATVSQLLRTYPVQDIAITMPPMEQVIAAIYQGKGVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QA1</accession>
    <name>Q67QA1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195288.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1157"/>
    <dbReference type="EnsemblBacteria" id="BAD40142">
        <property type="protein sequence ID" value="BAD40142"/>
        <property type="gene ID" value="STH1157"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1157"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_0_2_9"/>
    <dbReference type="OMA" id="GQELAWK"/>
    <dbReference type="OrthoDB" id="1567509at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026045">
        <property type="entry name" value="Ferric-bd"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002825">
        <property type="entry name" value="CfbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269615" evidence="3">
        <location>
            <begin position="28"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="356" mass="38292" checksum="30FAFC5AC6A0BF83" modified="2004-10-11" version="1">MNLRQRAAVALLSLCALVLSACGGSQQPPSNQPPSGGGQQQQQQEPQGSAEPKEIVVYTSHQADIHEPLFKAFEEATGIKVTPVYAATGELFQRMRAEANNPLGDVIFGGGAETHEANKDLLQPYVPKEIDKLAPGTVAKDNSWTGFTALPIVIMYNTNLLTADEAPKSFADLTDPKWAGKIAMPDAAKSGSAYTTVVTILHAMGRDDGKGWEVMKQIAANAKILASSSQGPKGTNDGEYAVSLTHEEAAFKYVAAGGPVGIVYPAEGTSNVPDAVAIIKAAKHPENAKAFIDFMVSQQMQEYVAKELNRRPVRTDVAPPEGLEDVSKIKFLDYDMDWAANQREAVLDQWKDIVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC4</accession>
    <name>Q67QC4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1134"/>
    <dbReference type="EnsemblBacteria" id="BAD40119">
        <property type="protein sequence ID" value="BAD40119"/>
        <property type="gene ID" value="STH1134"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1134"/>
    <dbReference type="eggNOG" id="COG3386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1026467_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="271" mass="29506" checksum="4401B4C947DAE4F6" modified="2004-10-11" version="1">MVNFASLAGADSGPTSFELSAIFERLPDSTEPVVLDRTDKVTLLAKLDSNSPQFWIELPDGGRKSQYGWSPVLAADGSVIFVDSEDLSLKKLLINGDEQQLLPPGSAAGFLRISSDRKYLGYAIPINLSPGSNWVGLYGAGIMDLETGKVLVSRTKENFFTSPIGWYGDQLIVHEWDATQTQDSLDLYALSLDGELTKFALGLPQANSYPEISPDHQWLVYADTHGDAILLRLADGSFGTVPNVEFPSWTQNGLTGLVDGRRVLIKITSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QL0</accession>
    <name>Q67QL0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195180.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1048"/>
    <dbReference type="EnsemblBacteria" id="BAD40033">
        <property type="protein sequence ID" value="BAD40033"/>
        <property type="gene ID" value="STH1048"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1048"/>
    <dbReference type="HOGENOM" id="CLU_2319086_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="99" mass="11109" checksum="1B0815A40D097B33" modified="2004-10-11" version="1">MEQDGRRAPGRDGGGRLTEQELYFVTRALDAELLALQKNDHYAEEMADENARRLMQEHADIHHRRVRLLLGLLDAPDDITTHAKLLLQSGEGVQAGAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP1</accession>
    <name>Q67QP1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1017"/>
    <dbReference type="EnsemblBacteria" id="BAD40002">
        <property type="protein sequence ID" value="BAD40002"/>
        <property type="gene ID" value="STH1017"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1017"/>
    <dbReference type="HOGENOM" id="CLU_3174056_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="47" mass="5312" checksum="9F33C73E0B87E911" modified="2004-10-11" version="1">MLSLVLIRLRGQDRVDPADWARIRMPDCRVVDTDAMQVVQPFGAWGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QQ3</accession>
    <name>IF11_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor IF-1 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">infA1</name>
        <name type="ordered locus">STH1005</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195137.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QQ3"/>
    <dbReference type="SMR" id="Q67QQ3"/>
    <dbReference type="STRING" id="292459.STH1005"/>
    <dbReference type="EnsemblBacteria" id="BAD39990">
        <property type="protein sequence ID" value="BAD39990"/>
        <property type="gene ID" value="STH1005"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1005"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_1_0_9"/>
    <dbReference type="OrthoDB" id="2066663at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000095887" description="Translation initiation factor IF-1 1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <sequence length="75" mass="8736" checksum="08ECF0D54CA139ED" modified="2004-10-11" version="1">MARSDMIEVDGVIVEPRSNGFFTVRLDLENQPEVIAHLGGKLRRHYIRVVPGDRVTVELSPYDLTRGRIVYRYRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QS5</accession>
    <name>Q67QS5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe-S_biosyn domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195115.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH983"/>
    <dbReference type="EnsemblBacteria" id="BAD39968">
        <property type="protein sequence ID" value="BAD39968"/>
        <property type="gene ID" value="STH983"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH983"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_2_9"/>
    <dbReference type="OMA" id="YQLYFDE"/>
    <dbReference type="OrthoDB" id="1891602at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="124" mass="12953" checksum="C739BE92C6EA8CA9" modified="2004-10-11" version="1">MNITLTEKASNRLKEIIKSKGENLALRVLVRPGGCSGFEYGMALERNPRPDDNISEFDGIKVVIDPASLPYLDGSTIDYVDSLMGGGFAINNPNAVTGCACGQSFRTANAAGAPRSCGSGGCAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QU1</accession>
    <name>Q67QU1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195099.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH967"/>
    <dbReference type="EnsemblBacteria" id="BAD39952">
        <property type="protein sequence ID" value="BAD39952"/>
        <property type="gene ID" value="STH967"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH967"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1194393_0_0_9"/>
    <dbReference type="OMA" id="FQWIRAS"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="24811" checksum="D830F748F8D4D67E" modified="2004-10-11" version="1">MIKALFFAPQALAYGPDEPDLLAGILQAEGYAVDRERVEKALAALPAAVREAGRAVRTPEEERAYHRMLWSALLPLVGHPAPDPALVSRLGAARDDYAAWWSLHPETLPVLNELHRRGFFLGAVGRWAPSLSRFLAEFEIAGFFQWIRASAAAGVAWPDPQLLREAVAGAGCGPAEALYCAASVRDDVSCAQAAGVTPVWVNRTGIVTGHEVIAVSDLRGLLVLLGDGGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV4</accession>
    <name>Q67QV4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH954</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195086.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH954"/>
    <dbReference type="EnsemblBacteria" id="BAD39939">
        <property type="protein sequence ID" value="BAD39939"/>
        <property type="gene ID" value="STH954"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH954"/>
    <dbReference type="eggNOG" id="COG2723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001859_0_2_9"/>
    <dbReference type="OMA" id="QGEYPQY"/>
    <dbReference type="OrthoDB" id="654705at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="479" mass="55235" checksum="BB91FF97D741C93B" modified="2004-10-11" version="1">MSGVPFLFPDQFLFGGAIAANQAEGAFDKDGKGLSIADVHPYVPVKSRDDRKEDATVKDSRDALRIVPGLHYPKQRGIDFYYTFRQDLALMKECGLQCFRTSFNWARIFPRGDERTPNEAGLAYYDQLIDAIIENGMEPVMTISHYEMPLALCLEYGGWYNRKLVDFYARFCEVLFERYHSKVKYWITFNQINLTTFNSLGILGEDHAHMLEATYQAVHHQFLAQAHAKRISRRYPGLMVGTMLSDKIAHPATCKPEDVLFSYRKNQMQYFFGDVAMRGSYPGYALRFFRDHGIRIAMEPGDLDLLAQHRMDFLSFSYYYTKINDAARDSWDPSDKSVNPYLPQSEWGWEIDPLGLRTALNQYYDRYQCPLFIVENGLGARDAVEADGRVHDSYRIAYIAEHLRQVAEAIRDGVDVMGYCLWSPIDIVSCSSAEMEKRYGLIYVDLDNLGRGTGQRIRKDSFFWYRDVIASRGRTLYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R74</accession>
    <name>Q67R74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative rRNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH834</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194967.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH834"/>
    <dbReference type="EnsemblBacteria" id="BAD39819">
        <property type="protein sequence ID" value="BAD39819"/>
        <property type="gene ID" value="STH834"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH834"/>
    <dbReference type="eggNOG" id="COG0144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_6_1_9"/>
    <dbReference type="OMA" id="PWCAEGF"/>
    <dbReference type="OrthoDB" id="1064993at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027391">
        <property type="entry name" value="Nol1_Nop2_Fmu_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031340">
        <property type="entry name" value="RsmF_methylt_CI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13636">
        <property type="entry name" value="Methyltranf_PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17126">
        <property type="entry name" value="RsmF_methylt_CI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="36"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD39819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="492" mass="53944" checksum="CD3F2D522F5BC446" modified="2004-10-11" version="1">MFESPDKGVVGLEDRALPQPFVARMSALLQDEFPAFLATYSSPPERGIRANRLKIAREELAERMGLTAEPVPWNPDGLYCPPSLRPGWHPYHAAGLYYGQEPSAMVVAPLVGARPGERVLDLAAAPGGKTTQLAAQMENRGLLVANEVDPGRVRALVENLERLGITCAAVVSERPERLAERLPGFFDRVLVDAPCSGEGMFRKTPEVRDRWRPDMPEACARVQGEILNSAVELLRPGGRLVYSTCTFAPEENEEVLLQLLTRRADLRMLPLPPVPGIAPGRPEWTRDPARAATVGVGLAGRLWPHRVRGEGHFVAVLERVADGAGRQRPPLRRDWQRPSKEALADFRTFCRETLTPAGQDALPPEAELRQHGEWLFAPPADSRALVGMHVVRAGLQLGRALRGRFEPSHPLALALRPGHVQRVRDLAAGSPELLAYLRGETLPAGGERGWTLVTVDGFPLGWAKAANGILKNHYPKVLRWDAGAPEPIDPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R79</accession>
    <name>Q67R79_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative mercury ion binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194962.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH829"/>
    <dbReference type="EnsemblBacteria" id="BAD39814">
        <property type="protein sequence ID" value="BAD39814"/>
        <property type="gene ID" value="STH829"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH829"/>
    <dbReference type="eggNOG" id="COG2608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134973_10_4_9"/>
    <dbReference type="OMA" id="AMHEETI"/>
    <dbReference type="OrthoDB" id="2061355at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="6786" checksum="6F734BB6D9646DEF" modified="2004-10-11" version="1">MTTVKIAGMSCNHCVMGVKKALSAIPGIENLQVEIGAARLEGNVDLEAVKRAIEEEGYEVVEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R87</accession>
    <name>Q67R87_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Branched chain amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH821</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH821"/>
    <dbReference type="EnsemblBacteria" id="BAD39806">
        <property type="protein sequence ID" value="BAD39806"/>
        <property type="gene ID" value="STH821"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH821"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_1_9"/>
    <dbReference type="OMA" id="PEYTKQG"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269947" evidence="2">
        <location>
            <begin position="32"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="3">
        <location>
            <begin position="64"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="414" mass="43612" checksum="36D08F6EF6082A58" modified="2004-10-11" version="1">MRGVSMKRLLVGLLAVSLLVLAACGGSQSQAPGGSTSQGGTPQGGTSAGSSSSGPSTSAGDKVIKIGLIAPLTGSVKTFGESSQKGFRLALEQAGYKAGDFTIEVVEGDDKGDATEGQNLATRFITRDGVKAIVGSITSGVTIPVSRIANENKIPMITGTATAEGVTVEEDGTRKPFIFRACFIDPFQGQVAARFALQELGVKTAAVFYDRGNPYTVGLAESFRAEFEAGGGQIVAWEAYTEADTDFSAVMTNVAALKPELLYLPDYYNKVSLLAKAARDKGLGDVPMLGGDGWDSEDLDFEALAGNYFTAHYSADDPSPAVQKFVQEFEAKYGHKPDSFAALTYDATNILLEAIRVANTDDTDAIRQAMQDLKGFQAVGGSISFDEKGNPIKAATILRVNPDKTYEFVTTVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RB2</accession>
    <name>Q67RB2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PTS system fructose-specific IIBC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">STH796</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH796"/>
    <dbReference type="EnsemblBacteria" id="BAD39781">
        <property type="protein sequence ID" value="BAD39781"/>
        <property type="gene ID" value="STH796"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH796"/>
    <dbReference type="eggNOG" id="COG1299">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1445">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013155_0_0_9"/>
    <dbReference type="OMA" id="TMEGLMP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022877">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05569">
        <property type="entry name" value="PTS_IIB_fructose"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013011">
        <property type="entry name" value="PTS_EIIB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003501">
        <property type="entry name" value="PTS_EIIB_2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013014">
        <property type="entry name" value="PTS_EIIC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003353">
        <property type="entry name" value="PTS_IIB_fruc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006327">
        <property type="entry name" value="PTS_IIC_fruc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02302">
        <property type="entry name" value="PTS_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00829">
        <property type="entry name" value="FRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01427">
        <property type="entry name" value="PTS_IIC_fructo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51099">
        <property type="entry name" value="PTS_EIIB_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51104">
        <property type="entry name" value="PTS_EIIC_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269630" evidence="13">
        <location>
            <begin position="25"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-2" evidence="14">
        <location>
            <begin position="8"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-2" evidence="15">
        <location>
            <begin position="127"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="BAD39781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="457" mass="47350" checksum="DC752664E2A0258B" modified="2004-10-11" version="1">MALVAKKLVAVTSCPTGIAHTYMAAEALQKAAAARQLSIKVETRGSIGAENVLTDEDIREADAVIIAADTAVDTGRFAGKPLVKVGVSEAIKDPAGLIDRALAAKPQDLVARVEEIKQERKSQATGWYKHLMTGVSYMIPFTVAGGILIAISFMFGIEAAATEGTFAYVLNVIGGQAGFGFMIPILAGFIAYSIADRPGIAPGMAAGFLANSVGAGFLGAIVGGFLAGYLVYYLNKWIRLPRTLEGIKPVLVLPLLGVGITGLIMVYVLNEPMAAIMAGLQNWLTGLRGGSAVVLGFILGAMMAFDMGGPVNKAAYAFSTGLLASQIYEPMAAVMAAGMTPPLGLFLATLLAPKKYTGEEREAGKAAAVLGISFITEGAIPFAAADPFRVIPAIMAGSAVTGALSMLFGCQLKVPHGGIWVLPMGNVVTNLGMYAVAIVVGTVITALLVNLLKPNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD1</accession>
    <name>Q67RD1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-1-phosphate thymidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH777"/>
    <dbReference type="EnsemblBacteria" id="BAD39762">
        <property type="protein sequence ID" value="BAD39762"/>
        <property type="gene ID" value="STH777"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH777"/>
    <dbReference type="eggNOG" id="COG0663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_9"/>
    <dbReference type="OMA" id="ITHAIQW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04189">
        <property type="entry name" value="G1P_TT_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005908">
        <property type="entry name" value="G1P_thy_trans_l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="364" mass="37642" checksum="C0899CA825AE62E3" modified="2004-10-11" version="1">MERGKAGMDVRALVLCASRGTRLRPLTHTWAKASLPVAGRPIWQHIFSYLAAHGFDEIGVVVGPGQEELARSVKRWSPQRVTVILQAEPAGIAHAVAAARPFLADQPFLLYLGDNLTNADLGPALRRFRAEAPSALITLQRVADPRTFGVAVLDGGRVTAVAEKPAEPTSDLAIAGIYFFSPEVHAAIAGLTPSARGELEITDAIARLIAGGRIVLGHELSGWWVDAGTAAGLLAANARLLEALAPGVDPTAVLEGVEMEGPVVVGPSAVLRRVRLRGPAFVGPGCRLTDAEVGPATSVGAGAELEGVRVWNSILLPGSRLAGPGLCLADSVVGAGATVSASPEGPATLVVGDDAHLAIPPRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE6</accession>
    <name>Q67RE6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH762"/>
    <dbReference type="EnsemblBacteria" id="BAD39747">
        <property type="protein sequence ID" value="BAD39747"/>
        <property type="gene ID" value="STH762"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH762"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_1_9"/>
    <dbReference type="OMA" id="GLEWINW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="57"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="283" mass="30476" checksum="BCF60B259A5EB355" modified="2004-10-11" version="1">MDAASHWLFLLAIVVALAVLAILLWDLLSTGLPRLSWSFLTSHASRFADRTGILAPLVGSFYVIVLTAAFALPVGVATAVFLEFYAEGDRPSPLRQSLTQFLKLNIDNLAGVPSIVYGLFGLAFFVRWMQFGRSILAAALTLALMVLPVIIVNTREAIRAVPPSLAHGAYALGASKTQVIFTVILPAALGGILTATILSVSRALGESAPLLAVGAWVYVTSLPRTPLDSFTVLPVQIYYWAGMPQKAFQEVASAGILVLLALLLSLNGIAIWLRNKYQKKAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RH3</accession>
    <name>Q67RH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH735</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194868.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH735"/>
    <dbReference type="EnsemblBacteria" id="BAD39720">
        <property type="protein sequence ID" value="BAD39720"/>
        <property type="gene ID" value="STH735"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH735"/>
    <dbReference type="eggNOG" id="COG1333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034630_1_0_9"/>
    <dbReference type="OMA" id="AHTNKNW"/>
    <dbReference type="OrthoDB" id="1518243at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023494">
        <property type="entry name" value="Cyt_c_bgen_Ccs1/CcsB/ResB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007816">
        <property type="entry name" value="ResB-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566">
        <property type="entry name" value="PTHR31566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05140">
        <property type="entry name" value="ResB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="ResB" evidence="7">
        <location>
            <begin position="23"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="ResB" evidence="7">
        <location>
            <begin position="373"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="471" mass="53224" checksum="6C829A7653A437A5" modified="2004-10-11" version="1">MDGQDVKPRKDLLDQTWDFFASVRVTTVLLFLLAVASIGGTLIEQEGMYASNLPPEEYYPQRYGPVLGMLLLRTGMTHAYSSWWYLTLLFLIAAALVICSIERLVPLWRAVYRPNPTPDKEFVRRLRQGFTFPARGEQPLAELAAALRARRYLVIERDGRLYADKGRLGRLGPYITHIGLLFIMIGAGMRAVPGVYLEQYIWVRDGEIVKVPGSDFYVESLGFAVEFYETGQPRSYQTHTRVIDAGGNVVKTETIAMNEPLTVGRVQLYQSSYYAEYGHAEVALVDRQSGAEIDRFTLDLSQPQRAYTVGDYRLQLLAYYPDFTVGEDGRPTTRSSQVNNPGLVLEVTPPDGEPFTTWFFPLYPGMEFDDTTPVRFLTQDVSPVYTSGLQVKKDLGLPVIYLGLIITTAGAFATFYLAHRRFWAFAEGGQVIVGGWTNRNAGSFEREMRQIAHQLDPERYATESDMEGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL1</accession>
    <name>Q67RL1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194830.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH697"/>
    <dbReference type="ESTHER" id="symth-q67rl1">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.B04"/>
    <dbReference type="EnsemblBacteria" id="BAD39682">
        <property type="protein sequence ID" value="BAD39682"/>
        <property type="gene ID" value="STH697"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH697"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_739124_0_0_9"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269953" description="Hydrolase_4 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="84"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="373" mass="39396" checksum="10F7E74078A44A0E" modified="2004-10-11" version="1">MRKLTAALLALLLLAGCAPGDGPAGGEIRRRALTIALDETWTAAAELTYPAGGDGPWPAVILFHGSGPMDMDATNVDAGGRVVSANFRLLAERLARSGYVVLRFHKRGVRRPGDYDPEQAYRATTNQLIADAGRVIAAAREQPEVDGGRIYLYGWSQGAQVAAHAALADRGVAGVILQGPPTGGWAETLRYQHLELGLRLLAEVDADGDGKLSAREWMDVPPGPAALMGSFYVWAPDSTPWASKMRADTDRDGDGLIDLEAELRPAIEALIANPAVNPMMDPANEPAESLAEIAPRLGRPVLILHGEHDGFVPVSDGEAIAAAAPETATLRRLPGLGHALSPTADPARDEFGPMDEAAIAALLEWLQAQAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RM9</accession>
    <name>Q67RM9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH679</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194812.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH679"/>
    <dbReference type="EnsemblBacteria" id="BAD39664">
        <property type="protein sequence ID" value="BAD39664"/>
        <property type="gene ID" value="STH679"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH679"/>
    <dbReference type="eggNOG" id="ENOG502ZVBX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1676959_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17920" checksum="5D83897A11CCC648" modified="2004-10-11" version="1">MREQLLAARNLVMGTTNILLLAVPDGWRVLEGPSQPEIDRWSERADQRWMSEGRATYRLVAVAPEAPEFARAEVELRLTATPSAPDVHDVRHRFTTVRRGLLPRREVPAAEVEIDCPFTGRRLRLELSPAMRGNRPTARPEDLQRLVHTMLEGLRCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RV3</accession>
    <name>Q67RV3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH605</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194738.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH605"/>
    <dbReference type="EnsemblBacteria" id="BAD39590">
        <property type="protein sequence ID" value="BAD39590"/>
        <property type="gene ID" value="STH605"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH605"/>
    <dbReference type="eggNOG" id="COG1820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032482_2_1_9"/>
    <dbReference type="OMA" id="YMKNPRL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="PTHR11113:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="8">
        <location>
            <begin position="54"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="308"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="385" mass="41106" checksum="A0A9A147896AAB9A" modified="2004-10-11" version="1">MKRWFRAPLILPDRVIPDGLLAYDAGRITDVWDLADPAAPPVPAEAEVVERGYLAPGYLDIHVHGGGGGDFMDADPEAVVAITTIHARHGTVGLLATTLTAPEEQIIRAIRTVRQAPRKGARVLGYHIEGPYINLAHKGAQNPEYVRPASIAEIDRWMAEGGPEDRWHVTLAPETDGALEAIRYLVRRGATVSAGHTDATYDQMRAAVEAGLSHATHLYNGMRGLHHREPGTVGSALALPGVTVELIADGVHVHPAAMQVAVRARGPERVLLVTDAMRATGMGDGEFTLGGLPVTVRNGEARLHSGSLAGSVLTMDRAVQNAVRLIGLDLPTAVAMATLHPARLLRLDDRKGSLAVGKDADLLVLDEDLNVKATIIGGEVVYDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RW1</accession>
    <name>Q67RW1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194730.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH597"/>
    <dbReference type="EnsemblBacteria" id="BAD39582">
        <property type="protein sequence ID" value="BAD39582"/>
        <property type="gene ID" value="STH597"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH597"/>
    <dbReference type="eggNOG" id="COG2384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071037_1_0_9"/>
    <dbReference type="OMA" id="GTDHGYI"/>
    <dbReference type="OrthoDB" id="1627266at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006901">
        <property type="entry name" value="TrmK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04816">
        <property type="entry name" value="TrmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018637">
        <property type="entry name" value="TrmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="231" mass="24347" checksum="E018452551173176" modified="2004-10-11" version="1">MIQLSPRLAAVARHVPAGRPFADIGTDHAYLPVHLVQSGRVPRAVAGDLLPGPLQAARATVAAAGLADRIDVRLGSGLTVLAPGEAPCVAVCGMGGPLIARILAEGPLAGVERLVLQPMGGEAQLRRWLADNGWRLVAEEVVEDGGRLYPVLVAEPGRMGLTRMEEEVGPLLLRQGGPLVVRYVDEKLELARRALAGASRSDQPAAQERAAQLSELVELLARALRICAGDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-04" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ3</accession>
    <name>DEOC_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="1">DERA</shortName>
            <ecNumber evidence="1">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="1">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">deoC</name>
        <name type="ordered locus">STH565</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-deoxy-D-ribose 5-phosphate = acetaldehyde + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194699.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67RZ3"/>
    <dbReference type="SMR" id="Q67RZ3"/>
    <dbReference type="STRING" id="292459.STH565"/>
    <dbReference type="EnsemblBacteria" id="BAD39550">
        <property type="protein sequence ID" value="BAD39550"/>
        <property type="gene ID" value="STH565"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH565"/>
    <dbReference type="eggNOG" id="COG0274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053595_0_1_9"/>
    <dbReference type="OMA" id="MNACIPP"/>
    <dbReference type="OrthoDB" id="1227369at2"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="PTHR10889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="PTHR10889:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000231569" description="Deoxyribose-phosphate aldolase">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="1">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <sequence length="220" mass="22871" checksum="02B139F10EA5E369" modified="2004-10-11" version="1">MTRRELARMIDHTLLKPDATEAQIRQLCAEGREHGFMSVCVNPYWVPLCAELLSGTEVKVCTTIGFPLGANRSEIKAVEAEDAVARGAREVDMVINVGALKSGRRDVVLNDIRAVVSAVAGEALVKVIIETGLLTDEEKVIACQLAQEAGADFVKTSTGFGTGGATVHDIALMRRTVGAGMGVKASGGVRDLETALAMIGAGANRIGASAGVAILAGLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S00</accession>
    <name>Q67S00_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH558"/>
    <dbReference type="EnsemblBacteria" id="BAD39543">
        <property type="protein sequence ID" value="BAD39543"/>
        <property type="gene ID" value="STH558"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH558"/>
    <dbReference type="eggNOG" id="ENOG502Z8E9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051800_0_0_9"/>
    <dbReference type="OMA" id="MGEHIRT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019733">
        <property type="entry name" value="Uncharacterised_YhfT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10797">
        <property type="entry name" value="YhfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="423" mass="43784" checksum="4F1614825C28D7FE" modified="2004-10-11" version="1">METLQLVVAALMGGLAAFLAQQGIAVFNDGLRPLLPEFLEGRMNRRELALTSFALCFGLVIGFGIPFSLTSQIILIHSVFLATDIIGTSSPNKWLAAGLGAAWGVLLTIGLQALVDLFALLPVNFLDALGQVSSPITAAFAVFPALAVALHHGWKKGAITFALQMLARQIVVRVNPIQFGTASINLNAEGTALVIGMILLLVFAAREKAEVTADASLAAVFSDRVQRIKKNVLVLSIMGALVAAAANLGVVAGDPISLGLAAEGNIVDAGIAALARGIGFVPLVATTAVATGVYGPVGMTFVFAAGFFSPHWIVAAILGAVIVFLEVMLLDVIARFLDRFPGVRAAGEHIRTAMTKVLEIALLVGGMIAGNAMAPGLGYFIVAAIVVLNEAAGLPVNRLAIGPVAAILTGILVNILKVLTLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S11</accession>
    <name>Q67S11_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH547</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH547"/>
    <dbReference type="EnsemblBacteria" id="BAD39532">
        <property type="protein sequence ID" value="BAD39532"/>
        <property type="gene ID" value="STH547"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH547"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="238" mass="26007" checksum="3F85F56428A1F504" modified="2004-10-11" version="1">MRGVIELLHVTKRYGPVTALEDLTLEVHEGEIFGLLGPNGAGKTTTLRLVAGLARPTQGQVRVAGLDPWQRGEPVRRQMGVVLDGGLLYDSLTVRENLLLFAGLFGVREQAVRDAMVQMDIADLRDRLAGRLSKGQRQRVALARALLHGPRLLYLDEPTSGLDPLATDDLHRLIGNLRGQGITILLSSHDMAEVEALCDRVCILQRGRMIACGTPAELKARHGPSLTDAFIQLTRRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S26</accession>
    <name>Q67S26_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Putative diacylglycerol kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH532</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194666.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH532"/>
    <dbReference type="EnsemblBacteria" id="BAD39517">
        <property type="protein sequence ID" value="BAD39517"/>
        <property type="gene ID" value="STH532"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH532"/>
    <dbReference type="eggNOG" id="COG0818">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112343_2_1_9"/>
    <dbReference type="OMA" id="GIVHCIR"/>
    <dbReference type="OrthoDB" id="2028997at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14265">
        <property type="entry name" value="UDPK_IM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000829">
        <property type="entry name" value="DAGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036945">
        <property type="entry name" value="DAGK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033717">
        <property type="entry name" value="UDPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34299">
        <property type="entry name" value="PTHR34299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01219">
        <property type="entry name" value="DAGK_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD39517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="161" mass="17086" checksum="85D5515A2BF512A0" modified="2004-10-11" version="1">MSGRPFRHKALLPRFRDAGRGLVRAYREEPNLRFHVFAGTCAAVAAYAVGVSGWEVAYLGGTIALVLSAELVNTAVERATDLAAGGRRHPLAGAAKEVAAGAVLLTALHALFAATLLFALRRDLAESVQAVFRLLIHRPLWLLPPAIAGVLGLFGGSRHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S47</accession>
    <name>Q67S47_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH511"/>
    <dbReference type="EnsemblBacteria" id="BAD39496">
        <property type="protein sequence ID" value="BAD39496"/>
        <property type="gene ID" value="STH511"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH511"/>
    <dbReference type="HOGENOM" id="CLU_2208713_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="107" mass="11635" checksum="FF90754294B457F2" modified="2004-10-11" version="1">MTVGGAAVEPHERVQAYERLLFRRNAAAWTTIIAAFVVLPLAARGWDPRVAGLVALGIFGAYIWFIWPHWRCPQCGHPLGRPARIMRCAWCGAPLTPDTPGGGASGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S93</accession>
    <name>Q67S93_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH465"/>
    <dbReference type="EnsemblBacteria" id="BAD39450">
        <property type="protein sequence ID" value="BAD39450"/>
        <property type="gene ID" value="STH465"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH465"/>
    <dbReference type="HOGENOM" id="CLU_2588423_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="80" mass="8019" checksum="E8C366CAB84601B8" modified="2004-10-11" version="1">MDRLVVWNVDGLDPDEVDALEAWVFAGGTALVGGPLSGFRGTFQPAAEGTARPAAPHPATLGIRAVSVGSGRFGGARGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SC1</accession>
    <name>Q67SC1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Penicillin-binding protein 1A</fullName>
            <ecNumber evidence="8">2.4.1.129</ecNumber>
            <ecNumber evidence="7">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">STH437</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="8"/>
    <dbReference type="EC" id="3.4.16.4" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194571.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH437"/>
    <dbReference type="EnsemblBacteria" id="BAD39422">
        <property type="protein sequence ID" value="BAD39422"/>
        <property type="gene ID" value="STH437"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH437"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_7_9"/>
    <dbReference type="OMA" id="GYYMEEV"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269352" description="Penicillin-binding protein 1A" evidence="19">
        <location>
            <begin position="34"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="21">
        <location>
            <begin position="54"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="20">
        <location>
            <begin position="345"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="768"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="BAD39422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="799" mass="87851" checksum="C14F05FBA0B605B4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD0</accession>
    <name>Q67SD0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194562.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH428"/>
    <dbReference type="EnsemblBacteria" id="BAD39413">
        <property type="protein sequence ID" value="BAD39413"/>
        <property type="gene ID" value="STH428"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH428"/>
    <dbReference type="HOGENOM" id="CLU_2902613_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="62" mass="6581" checksum="1365F063CAA53B72" modified="2004-10-11" version="1">MRNRRNLLIWIATLLSAVGVFLLVVNGQMMLGLSAAVAVALFGAGRRACRLGPDSTPSLDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SF7</accession>
    <name>Q67SF7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194535.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH401"/>
    <dbReference type="EnsemblBacteria" id="BAD39386">
        <property type="protein sequence ID" value="BAD39386"/>
        <property type="gene ID" value="STH401"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH401"/>
    <dbReference type="HOGENOM" id="CLU_1214285_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="228" mass="24502" checksum="754537758CB33D34" modified="2004-10-11" version="1">MEIALVLPALVLLVIGGWVAGYMTFAKMALAMAANRAAREFAAAVSLHDRLKEPERAYRYDGGFAESFGLPRWAVHALIMRTQAAPGPSRSDQAVVVAMCYRLPLALPGGLPAGVRTAEAAAGSWREIRSEAEEWEALLRRAEALAARGEQLTGQARWAADLWRQVIEGPPADFTPLDGADLQGPEGLEQAARKLCEPPAGDGRSLIVTARAAYLLQTVFEPEGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SG9</accession>
    <name>Q67SG9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Septum site-determining protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">minD2</name>
        <name evidence="2" type="ordered locus">STH389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194523.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH389"/>
    <dbReference type="EnsemblBacteria" id="BAD39374">
        <property type="protein sequence ID" value="BAD39374"/>
        <property type="gene ID" value="STH389"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH389"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1045556_0_0_9"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="7"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="266" mass="27990" checksum="06EE5F4A4625F7AE" modified="2004-10-11" version="1">MAKGLVIALWSPKGGVGKTLLAVAVALRLSERHRTAIIDGNADNPDLVTLLQSAGLPNIGTWPGRVAPEEIENHLVRWASRLFVLPGPARFIDEAVLSGEAMEAAVRSLADAGMHVVVDLCTGLRDSTLVALDLADWVLMPVTPDLLALTPLKRIARELDFLRLPAGKFRIVVNRVSEGQEIGTADIRELSPFPVVGEVPSCRQAAQAINRGDLQAALAASTPVGKAAAALLRGIVETDAAPQPAAPPFGAGLLSRLWPIAGREGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SJ7</accession>
    <name>Q67SJ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Lon protease</fullName>
            <ecNumber evidence="5 6">3.4.21.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent protease La</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lon</name>
        <name evidence="13" type="ordered locus">STH361</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 9">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="5">By heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6 9">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="5 6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194495.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH361"/>
    <dbReference type="MEROPS" id="S16.001"/>
    <dbReference type="EnsemblBacteria" id="BAD39346">
        <property type="protein sequence ID" value="BAD39346"/>
        <property type="gene ID" value="STH361"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH361"/>
    <dbReference type="eggNOG" id="COG0466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004109_4_3_9"/>
    <dbReference type="OMA" id="GAWQVVD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01973">
        <property type="entry name" value="lon_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027543">
        <property type="entry name" value="Lon_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001174">
        <property type="entry name" value="Lon_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="12">
        <location>
            <begin position="12"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="11">
        <location>
            <begin position="595"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="781"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7 9">
        <location>
            <position position="682"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7 9">
        <location>
            <position position="725"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <begin position="358"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001174-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="BAD39346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="803" mass="90152" checksum="E231E5B6AFD70F13" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM2</accession>
    <name>Q67SM2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH336</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH336"/>
    <dbReference type="EnsemblBacteria" id="BAD39321">
        <property type="protein sequence ID" value="BAD39321"/>
        <property type="gene ID" value="STH336"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH336"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_18_11_9"/>
    <dbReference type="OMA" id="AMMFISS"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038109">
        <property type="entry name" value="DNA_bind_recomb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011109">
        <property type="entry name" value="DNA_bind_recombinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025827">
        <property type="entry name" value="Zn_ribbon_recom_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07508">
        <property type="entry name" value="Recombinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13408">
        <property type="entry name" value="Zn_ribbon_recom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51737">
        <property type="entry name" value="RECOMBINASE_DNA_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="7">
        <location>
            <begin position="35"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Recombinase" evidence="8">
        <location>
            <begin position="191"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="4 5">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="532" mass="60392" checksum="640BF3943698BAAD" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SQ5</accession>
    <name>Q67SQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH303"/>
    <dbReference type="EnsemblBacteria" id="BAD39288">
        <property type="protein sequence ID" value="BAD39288"/>
        <property type="gene ID" value="STH303"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH303"/>
    <dbReference type="eggNOG" id="COG3876">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033227_1_0_9"/>
    <dbReference type="OMA" id="YIWTLYD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033922">
        <property type="term" value="F:peptidoglycan beta-N-acetylmuramidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008302">
        <property type="entry name" value="NamZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42915">
        <property type="entry name" value="PTHR42915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07075">
        <property type="entry name" value="DUF1343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016719">
        <property type="entry name" value="UCP016719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="376" mass="41587" checksum="B099AC42AFBD2ED9" modified="2004-10-11" version="1">MLLAARKELITGVRVGLITNHTGTNSQLESLVDLLVADPAVRVTALYSPEHGIRGDAAAGEKVSSSTDPVTGLPVYSLYGEHRKPTPEMLREVDVLLFDIQDAGARFYTYTWTLALCMQAAAESGKRLVVLDRPNPIGGVQVEGGVVRPGLESFVGLYPVATRHGLTHGEIARWLNDRFGLGCDLTVVPMEGWRRTMWWDETGLPFVPPSPAANGLDMLTLYPGLCFLEGTNLSEGRGTALPFQVFGAPWLDERRTVAELKRRNLPGVLFRPAYFTPWTSKYQGQLCRGVQVHIVNRQAVRPVALGAHVLDVVRRLHPGEFQWVSWDGTRTVDRLSGSDLLYRAVEGELALEAVLAQWEEEARRFAAESAAYHLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SW7</accession>
    <name>Q67SW7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194376.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH241"/>
    <dbReference type="EnsemblBacteria" id="BAD39226">
        <property type="protein sequence ID" value="BAD39226"/>
        <property type="gene ID" value="STH241"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH241"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_9"/>
    <dbReference type="OMA" id="ECGSLDW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="33908" checksum="8279B85AF9FB99EC" modified="2004-10-11" version="1">MDVQGLEAFWWIAKTGSFNRAAERLFLTQPSVTARIQALEKELGQQLFERRPRGVRLTDAGRALLPHAERVLQDIKRARQAVLDLQTASGGTLSVGSALTTSAYLLPDILLRFKRGYPNVEVLVHTGRSHQVQQLVLDDTVQVGLVHAPLPSHAEIVSYPLSEEPILLVVPPDHPVARHSEVTLDELTQESFITTDRASGYWALVEQWWASQGFVPHITMELDSIEAAKRMVLCGLGLTMLPYSTVKSEIELSQVVAVPLANSATLKRQNILIHRRGKIWSGTAKAFLRVLEEMFQVNLGVKDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T45</accession>
    <name>Q67T45_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAGPA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194298.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH163"/>
    <dbReference type="EnsemblBacteria" id="BAD39148">
        <property type="protein sequence ID" value="BAD39148"/>
        <property type="gene ID" value="STH163"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH163"/>
    <dbReference type="eggNOG" id="COG4632">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004974_0_0_9"/>
    <dbReference type="OMA" id="WRFAVMS"/>
    <dbReference type="OrthoDB" id="1242748at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018711">
        <property type="entry name" value="NAGPA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09992">
        <property type="entry name" value="NAGPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269666" description="NAGPA domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="NAGPA" evidence="2">
        <location>
            <begin position="203"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="921" mass="97057" checksum="70D73AF79E147E03" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T49</accession>
    <name>Q67T49_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Medium-chain fatty-acid-CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH159"/>
    <dbReference type="EnsemblBacteria" id="BAD39144">
        <property type="protein sequence ID" value="BAD39144"/>
        <property type="gene ID" value="STH159"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH159"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_9"/>
    <dbReference type="OMA" id="FAIPSMG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="444"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="539" mass="60219" checksum="873C49D1B6FE22DC" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T61</accession>
    <name>Q67T61_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oligopeptide ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194282.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH147"/>
    <dbReference type="EnsemblBacteria" id="BAD39132">
        <property type="protein sequence ID" value="BAD39132"/>
        <property type="gene ID" value="STH147"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH147"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_8_6_9"/>
    <dbReference type="OMA" id="RANKANI"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269364" evidence="4">
        <location>
            <begin position="29"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="5">
        <location>
            <begin position="107"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD39132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="572" mass="63260" checksum="9A8D6C851B3943EA" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T99</accession>
    <name>Q67T99_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysulfide reductase subunit A, N-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH109</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194244.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH109"/>
    <dbReference type="EnsemblBacteria" id="BAD39094">
        <property type="protein sequence ID" value="BAD39094"/>
        <property type="gene ID" value="STH109"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH109"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747798_0_0_9"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10518">
        <property type="entry name" value="TAT_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269670" evidence="6">
        <location>
            <begin position="32"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="7">
        <location>
            <begin position="36"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD39094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="379" mass="40402" checksum="331B938BB4D7A188" modified="2004-10-11" version="1">MAELSRRDFLKLGGVAAGALAVGLPQRPARAAQADVRKVPTLCEMCTSRCGVFAVVENGRVTRIEGNPAHPVNLGRPCARGNAGASALYDPDRLKEPMKRGEDGKLYPITWEQAVEEIGAKLNEIRKRHGPEALVFAEYNNLNSTLTKRWTEAFGSPNHVGHAANCFANRNVGYSAVFGALPSVDYENVKFYLSPGRNLLGGIKVSEVAALAKAKANGARIVVLDPRHSELAGWGEWIPIKSAGDLAFLLAVANVLITEGIYNKAWVEAHCNGFEQLAEGIREYTPEWQEQHTDIPAEKVRQLAREMAAAAPATVVDPGWHGGNGMYWNGYEAARAGAIVNALLGNLGAKGGLKLSPKVALGTIDNPPEGAVLEAAGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC2</accession>
    <name>Q67TC2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">F-type ATPase subunit c</fullName>
            <shortName evidence="12">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">atpE</name>
        <name evidence="15" type="ordered locus">STH86</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ATPase C chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194221.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH86"/>
    <dbReference type="EnsemblBacteria" id="BAD39071">
        <property type="protein sequence ID" value="BAD39071"/>
        <property type="gene ID" value="STH86"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH86"/>
    <dbReference type="eggNOG" id="COG0636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_5_1_9"/>
    <dbReference type="OMA" id="NIATVGY"/>
    <dbReference type="OrthoDB" id="2078221at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005953">
        <property type="entry name" value="ATP_synth_csu_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="PTHR10031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01260">
        <property type="entry name" value="ATP_synt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269381" description="ATP synthase subunit c" evidence="13">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="14">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="12">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="BAD39071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="77" mass="7870" checksum="CF6DC2F4371A22BF" modified="2004-10-11" version="1">MTETAWIALAAALSISVAAIGATVAQGKATTAAMDAIWRQPEAANDVRGALIVSLALMEAIAIYGLLIGLLIIFMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TC6</accession>
    <name>Q67TC6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine 2-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH82</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-N-acetylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194217.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH82"/>
    <dbReference type="EnsemblBacteria" id="BAD39067">
        <property type="protein sequence ID" value="BAD39067"/>
        <property type="gene ID" value="STH82"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH82"/>
    <dbReference type="eggNOG" id="COG0381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041674_1_0_9"/>
    <dbReference type="OMA" id="RYNTERP"/>
    <dbReference type="OrthoDB" id="1117971at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase_2" evidence="2">
        <location>
            <begin position="19"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="380" mass="42421" checksum="6AE99E1056831510" modified="2004-10-11" version="1">MAIFGSRPEGIKMAPVVQALRRHPDWFETIVVSTGQHREQLDQVFAAFGLAADHDLAIMQPRQTLTDIMVRALSGLDELLARERPDLVLVHGDTSTTAAGALAAFYHKIPLGHVEAGLRTFNKYNPYPEEINRRIAGIIADIQFAPTPRAREHLIAEHIDPGTIFVTGQTGVDAALTVLAQPHTFTVPALRQVDFSRHRVVLMTAHRRENIGEPMRNMFRAVRDLVDRFPDVLLIYAVHLNPAVREIAWPILDGHPRILLLDPLPYPDMIHLAARSYMAMTDSGGIQEETPSMGVPHILMRETTERPEALEAGVILLSGTSYEGVYAAGERLLTDQALYQQMARARNPFGDGQASRRIAEYLGWYFGFTADRPEEFTPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD4</accession>
    <name>Q67TD4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Release factor glutamine methyltransferase</fullName>
            <shortName evidence="6">RF MTase</shortName>
            <ecNumber evidence="6">2.1.1.297</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N5-glutamine methyltransferase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-(glutamine-N5) MTase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-glutamine N-methyltransferase PrmC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prmC</name>
        <name evidence="9" type="ordered locus">STH74</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="6"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH74"/>
    <dbReference type="EnsemblBacteria" id="BAD39059">
        <property type="protein sequence ID" value="BAD39059"/>
        <property type="gene ID" value="STH74"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH74"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_3_1_9"/>
    <dbReference type="OMA" id="FDARYWE"/>
    <dbReference type="OrthoDB" id="1816476at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018364">
        <property type="term" value="P:peptidyl-glutamine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02126">
        <property type="entry name" value="RF_methyltr_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="21"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="136"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="141"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="210"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="305" mass="31642" checksum="6C0BBE76CFFB4093" modified="2004-10-11" version="1">MAAPREAAPDRAPDGPQTVGEALAQARAQLAAAGVADAEREAAWLLAHVLGCRAGALVARRSEPLAPEASDEYAALVARRAAREPLQYILGTEEFMGLTFRVTPAVLIPRLDTAALVEQAVARLTGGAAEARGVLRVADIGTGSGAIAVAVAHLLPHAQVVAVDLSPEALAVAAENARLNGVADRVRFRQGDLLAPLAEEGGRFAAILSNPPYIREDEIAGLMPEVRDWEPRLALVAGDDGLAFYRRLAREAPAFLEPGGFLGVEVGIGQAPAVAALFREAGLKDVAVCRDTAGVDRAVLGSVAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TE2</accession>
    <name>TAL_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transaldolase</fullName>
            <ecNumber evidence="1">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tal</name>
        <name type="ordered locus">STH66</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = beta-D-fructose 6-phosphate + D-erythrose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194201.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TE2"/>
    <dbReference type="SMR" id="Q67TE2"/>
    <dbReference type="STRING" id="292459.STH66"/>
    <dbReference type="EnsemblBacteria" id="BAD39051">
        <property type="protein sequence ID" value="BAD39051"/>
        <property type="gene ID" value="STH66"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH66"/>
    <dbReference type="eggNOG" id="COG0176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079764_0_0_9"/>
    <dbReference type="OMA" id="VRHPMHV"/>
    <dbReference type="OrthoDB" id="784333at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0570">Pentose shunt</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000126362" description="Probable transaldolase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00494"/>
        </source>
    </evidence>
    <sequence length="216" mass="23580" checksum="2E0AFCEC02DF73AE" modified="2004-10-11" version="1">MKLFIDTANVDDIREVASWGVLSGVTTNPSLVAKEGRDFMQVLREILEIVDGPISAEVISLQADDMVEEARQYYELHKNIVIKLPMTAEGLKACARLSAKGVRCNMTLIFSPNQALLCARAGAAFVSPFVGRLDDISTDGIQLIRDTAEIFDLHGIDTEIIAASIRTPGQVVEAAKAGAHIATIPPKVFHQMLKHPLTDSGIERFLKDWEAAKGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TF0</accession>
    <name>Q67TF0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative middle wall protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH58</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194193.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH58"/>
    <dbReference type="EnsemblBacteria" id="BAD39043">
        <property type="protein sequence ID" value="BAD39043"/>
        <property type="gene ID" value="STH58"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH58"/>
    <dbReference type="eggNOG" id="COG2373">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262217_0_0_9"/>
    <dbReference type="OrthoDB" id="149270at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008964">
        <property type="entry name" value="Invasin/intimin_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001119">
        <property type="entry name" value="SLH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00395">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49373">
        <property type="entry name" value="SSF49373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51272">
        <property type="entry name" value="SLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269384" evidence="2">
        <location>
            <begin position="30"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="SLH" evidence="3">
        <location>
            <begin position="85"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1297" mass="138093" checksum="138C5CCEA58B3B08" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-16" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK4</accession>
    <name>RECF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication and repair protein RecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recF</name>
        <name type="ordered locus">STH4</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194139.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67TK4"/>
    <dbReference type="SMR" id="Q67TK4"/>
    <dbReference type="STRING" id="292459.STH4"/>
    <dbReference type="EnsemblBacteria" id="BAD38989">
        <property type="protein sequence ID" value="BAD38989"/>
        <property type="gene ID" value="STH4"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH4"/>
    <dbReference type="eggNOG" id="COG1195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040267_0_1_9"/>
    <dbReference type="OMA" id="GESWSYA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00365">
        <property type="entry name" value="RecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001238">
        <property type="entry name" value="DNA-binding_RecF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018078">
        <property type="entry name" value="DNA-binding_RecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042174">
        <property type="entry name" value="RecF_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182:SF0">
        <property type="entry name" value="PTHR32182:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00611">
        <property type="entry name" value="recf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00617">
        <property type="entry name" value="RECF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00618">
        <property type="entry name" value="RECF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000236153" description="DNA replication and repair protein RecF">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00365"/>
        </source>
    </evidence>
    <sequence length="375" mass="42534" checksum="54AF6FF2E05C521C" modified="2004-10-11" version="1">MYLSTLQLGAFRNYDSLTIHFSPGLNVLYGDNAQGKTNLLEAIHFLATGRSHRTSRDPDMVQEGREELLARAAVVRRTGTIELELRCGLQTRKQLKINGIAERKIARLVGSLAVVLFSPDDLQLLKGPPSGRRRFLDLELSQISQTYLHHLMAYNRLVAQRNTLLKQPVIDEGLMAVYDEQLVETGAQLVVRRAEAVRRLSPIASRYHRMLAEDREDLELAYQSQGVGDDGAADLETVRRRLERELARLRSEERRRQVTLVGPHRDDVGFWVAGRDARLYASQGQQRTAVLALKLAELEFMSEEIGEPPLLLLDDVASELDPHRRHYLLSAVREGVQSFITCTDLEDLMVREWPADHRLFRVRAGTVVLDDRGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J58</accession>
    <name>Q67J58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative metalloendopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH3311</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3311"/>
    <dbReference type="MEROPS" id="M23.009"/>
    <dbReference type="EnsemblBacteria" id="BAD42292">
        <property type="protein sequence ID" value="BAD42292"/>
        <property type="gene ID" value="STH3311"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3311"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027710_2_1_9"/>
    <dbReference type="OMA" id="DIFASNP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011098">
        <property type="entry name" value="G5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07501">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01208">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51109">
        <property type="entry name" value="G5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269863" evidence="3">
        <location>
            <begin position="37"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="5">
        <location>
            <begin position="176"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="G5" evidence="4">
        <location>
            <begin position="227"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD42292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="435" mass="46855" checksum="E165F47DAED1E209" modified="2004-10-11" version="1">MPRRPLLLVVLAAIISLGLAADLGVRNLQRSQPAAAADGQETGTGTAGAEPDQLASADTTDRERLDIASRQAQGPRTARDPLGEPAAAQVVEMKKAAVITVNGEPVVAVADEAAAREVVDTILNSYRESYLSDATVVEELSFAEAVTWEEGEVAADSIKTVEEAINVLLLGTDKVATYTVASGDTAWDIALEYGLTTDQLAKANPGVDIELLQIGQQLTITYKEPYVHPQSVSQRVVYESIPFAEQRIEDDTLWPWESVVVTPGRSGTREKVIRERRQDGQIVSTEVLSSRVTAEPVTQVRRVGVKQVPDLGSGSLILPVVGEISSYYGPRWGTWHNGIDIAVPHGTPVRAADSGMVVYRGWNGNYGNMIEIDHGGGKMVTWYAHLSGFNVSVGQQVNKGDIIGYVGSTGYSTGPHLHFEIRIDGDPVNPLNYYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J69</accession>
    <name>Q67J69_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3300</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3300"/>
    <dbReference type="EnsemblBacteria" id="BAD42281">
        <property type="protein sequence ID" value="BAD42281"/>
        <property type="gene ID" value="STH3300"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3300"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_39_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="17"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="127"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="200"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="272"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="491" mass="53837" checksum="F84AF94FDA899149" modified="2004-10-11" version="1">MKEGTTIAAQAGERVPLPSWLRGLLDPLSEPVLLVGADGRIRYGNPSAGRLLGCSPEALPGLAVGAVLPDGEALLGRRQEDPSDTDAVRRDGGRIRVAVTARPGPEGLTQLLLRPVAEAPEGRLRESDPRYRAVVEDQTDLIRRFTPDGRLTFVNPAFCRYFGRSEEELLGSDFIRLIHPEDREVVHRQLAVLSPENPTTVTEARIPRPDGGVGWVQFVNRGIFDAEGRLVECQSVGRDITAQKEAEARIAEAREAVNRAARVTTLAIIGGGIVHEINQPLNAIRVLAETGLCLLVQADPPQADEIVAILSRISRQVDRIDSIVNHLRDVLKNSRNQRYEYCDLNEVVERALLLMDNRIQAHGIRLRLEKADQLPKVFGYSVNLEEVVLNLLNNAMQALDEVDWPHKEIAVRTWADDRVYLEVADNGPGFDPAIRERVLEPFFTTKSGSSLGMGLPIVKAIVENADGRLELADAHGGGAVVRVSLPIATPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J79</accession>
    <name>Q67J79_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH3290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197402.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3290"/>
    <dbReference type="EnsemblBacteria" id="BAD42271">
        <property type="protein sequence ID" value="BAD42271"/>
        <property type="gene ID" value="STH3290"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3290"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_9"/>
    <dbReference type="OMA" id="FTIIETV"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="8"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="8">
        <location>
            <begin position="130"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="130"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD42271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="232" mass="25459" checksum="61264B40C8965EFB" modified="2004-10-11" version="1">MAPGKRPVVLVVDDEPRMRELIRLYLAAECTVLEAGDGLTALRAVRDAGPDLVLLDVMMPRLDGWQTLQRIREESAVPVIMLTARGGVADRVQGLRMGADDYIAKPFDGRELAARVQAVLRRSAGQVEANVPIRRGSLTIRPAERTALYAGRPLPLTPKEFDLLALLAAHPGQVFSREKLLDRIWGPDFQGDARTVDAHIKNLRDKLGEGAGLILTVWGVGYRFAEVPDAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q67J80</accession>
    <name>Q67J80_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH3289</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197401.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3289"/>
    <dbReference type="EnsemblBacteria" id="BAD42270">
        <property type="protein sequence ID" value="BAD42270"/>
        <property type="gene ID" value="STH3289"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3289"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_9"/>
    <dbReference type="OMA" id="HGGQASC"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="177"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="237"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD42270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="448" mass="48599" checksum="7F75A466952AB45A" modified="2004-10-11" version="1">MRSLSLVARFWLSLSLLLLVVLVPMEYALDQLLERFYATQVTEPLLYHGRQLADMLAAEDAALAAAPLMAEMVGGEVVVLDREGRPRSFPGASAVEVPEDIRRAVVVGRTHVDQLRTPEGATYIVTGVPVQGGEGGVLLLAPADPLRQSFDRARRGLWLAGAAALLLGTGLALVMARSLTRPVLAMERATRAIARGDFSARVAPAGANELGRLGEAINRMAEQLEGHERRRREFLADVAHELRTPLSYIRGYTQALAEGVVTDPAEVRRFQRVVMDEVDRLSRLVDDLMDLAQIEEGQMAFERVPLDLRVPVEQAVRTVRSQAEEKGVALVVGLPDALPPVLADGGRIQQVVFNLLDNALRHTPPGGVVRVSAAVTRDAVAVRVRDTGEGIDPAQLPHVFDRFRGRRKGGRGLGLAVVRSIVRAHGGDVGVESRPGGGAAFWFWLPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JD6</accession>
    <name>Q67JD6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stage V sprulation protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">spoVB</name>
        <name evidence="8" type="ordered locus">STH3232</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197345.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3232"/>
    <dbReference type="EnsemblBacteria" id="BAD42214">
        <property type="protein sequence ID" value="BAD42214"/>
        <property type="gene ID" value="STH3232"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3232"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_2_1_9"/>
    <dbReference type="OMA" id="QYTVADV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13124">
        <property type="entry name" value="MATE_SpoVB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024923">
        <property type="entry name" value="PG_synth_SpoVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038958">
        <property type="entry name" value="PG_synth_SpoVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="364"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="536" mass="57383" checksum="0FCA88A06E73E909" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JH7</accession>
    <name>HUTI_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazolonepropionase</fullName>
            <ecNumber evidence="1">3.5.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazolone-5-propionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutI</name>
        <name type="ordered locus">STH3191</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolytic cleavage of the carbon-nitrogen bond in imidazolone-5-propanoate to yield N-formimidoyl-L-glutamate. It is the third step in the universal histidine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="3.5.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc or iron ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. HutI family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JH7"/>
    <dbReference type="SMR" id="Q67JH7"/>
    <dbReference type="STRING" id="292459.STH3191"/>
    <dbReference type="EnsemblBacteria" id="BAD42173">
        <property type="protein sequence ID" value="BAD42173"/>
        <property type="gene ID" value="STH3191"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3191"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041647_0_1_9"/>
    <dbReference type="OMA" id="CAPHARW"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00551"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01296">
        <property type="entry name" value="Imidazolone-5PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00372">
        <property type="entry name" value="HutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="PTHR42752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000306531" description="Imidazolonepropionase">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00372"/>
        </source>
    </evidence>
    <sequence length="425" mass="44842" checksum="319A7906D27B57DA" modified="2004-10-11" version="1">MRPVDLLIVGAGELVTMAGGPRRGERMKDAAVIPGGALAARDGRIVAVGPEDEVLRTVETGPDTRVIDARGRAVIPGFVDPHTHLCFAGDRAEEFALRLGGATYQEIAARGGGILETVRATRAASQAELVELGLARLDQLALNGTTTVEVKSGYGLSLADELKQLRAIRAMARRHPLTVVPTFMGAHEVPPEYRSRREAYVDLLVEEMLPAVAAEPGLARFADVFTEAGVFSVAESRRILERAKALGFGVKVHADELSDLGGAALAAELGAISAEHLLHASDEALAKLAEAGTVAVCLPGTSFCLMNAPYARARRMIELGCTVALGSDYNPGSCPAYAMPFIITLACMHLGLNPSEALAAATINAAAAIGMEAEVGSLEVGKLADVVILSTPTHWHIPYHMGMGVVAKVVKRGRLIVDEGKVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JK6</accession>
    <name>Q67JK6_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH3162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197275.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3162"/>
    <dbReference type="EnsemblBacteria" id="BAD42144">
        <property type="protein sequence ID" value="BAD42144"/>
        <property type="gene ID" value="STH3162"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3162"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_9"/>
    <dbReference type="OMA" id="NVLRKTQ"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="154"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD42144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="221" mass="24184" checksum="5963C21BBA1505E6" modified="2004-10-11" version="1">MDGRKIRIMIVDDQSLLRRGLAALLNRNPDMEVVGEASSGEEALRMAAELKPDVILMDVRMPGMDGVTATRELVRSGTAAGVIILTTFDDDEYIFEGLAAGARGYLLKDAEYDELSQAIRTVAAGESLLQPQITTRVLKEFSRLSSMAASRPKARPLVEPLTERETEVLRLMASGASNQDIAEKLFIGQGTVKHHVRSIFAKLGARDRVHAILLAQELNLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67JU6</accession>
    <name>RL2_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplB</name>
        <name type="ordered locus">STH3072</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD42054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197187.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67JU6"/>
    <dbReference type="SMR" id="Q67JU6"/>
    <dbReference type="STRING" id="292459.STH3072"/>
    <dbReference type="EnsemblBacteria" id="BAD42054">
        <property type="protein sequence ID" value="BAD42054"/>
        <property type="gene ID" value="STH3072"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3072"/>
    <dbReference type="eggNOG" id="COG0090">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_2_1_9"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="961486at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000237251" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="277" mass="30602" checksum="EA60C986BB1BED0A" modified="2004-10-11" version="1">MGIKHYKPTSPGIRQMTRHTFEELTSTRPEKSLLERLTRKAGRNVYGRITVRHRGGGHKRMYRIIDFKRNEFDGIPAKVVSIEYDPNRTCRIAKVVYANGAKRYILHPVGLNVGDTVVSGPDADIKVGNALPLENIPVGTIVHNIELYPGRGGQLVRSAGASAQIMAKEGRYALLRLPSGEQRKVLLACRATVGQVGNLEHENIVIGKAGRKRHLGFRPAVRGVVMNPVDHPHGGGEGRSPIGMPSPVTPWGKPTLGYKTRKPNKKSDRLIVSRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K11</accession>
    <name>Q67K11_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Flagellar basal body rod protein FlgB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flgB</name>
        <name evidence="7" type="ordered locus">STH3006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197121.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH3006"/>
    <dbReference type="EnsemblBacteria" id="BAD41989">
        <property type="protein sequence ID" value="BAD41989"/>
        <property type="gene ID" value="STH3006"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH3006"/>
    <dbReference type="eggNOG" id="COG1815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125463_3_2_9"/>
    <dbReference type="OMA" id="NIQAHHF"/>
    <dbReference type="OrthoDB" id="1914857at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006300">
        <property type="entry name" value="FlgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30435:SF12">
        <property type="entry name" value="PTHR30435:SF12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002889">
        <property type="entry name" value="Rod_FlgB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="119" mass="13261" checksum="B7BCF7DBC8D89EBC" modified="2004-10-11" version="1">MIGRDLAARIVEQAIDAASLRQRVLANNLANAMTPGFKRSRVDFETELARALAKGGDPDQVRPTVVRETDSIGRPDGNNVDVEAESVKMAANQIWYAALTRQINDHFDRLRMVITEGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K19</accession>
    <name>Q67K19_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MgtE_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2998</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197113.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2998"/>
    <dbReference type="EnsemblBacteria" id="BAD41981">
        <property type="protein sequence ID" value="BAD41981"/>
        <property type="gene ID" value="STH2998"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2998"/>
    <dbReference type="HOGENOM" id="CLU_1234490_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006668">
        <property type="entry name" value="Mg_transptr_MgtE_intracell_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038076">
        <property type="entry name" value="MgtE_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03448">
        <property type="entry name" value="MgtE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="MgtE_N" evidence="4">
        <location>
            <begin position="163"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="106"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="224" mass="24385" checksum="78E84BD009CACAC7" modified="2004-10-11" version="1">MSAVAPEPTSTPGEELPRKRRLRLPRLRRRRAEEAGAEPPPEPRRRPRIRPRRPRQPPSIAVYLTLFALLTGAAAITLHLRPDLIPGLTPVEEAPGQAPADAAPSAEQLQRAVELTRKQADLLQREADLNQREAALQEREAQVDQLLEELGAAGRSGATAVQTARLLTEMSPYRAAVLLAEMGNGTAVAVLKQMKTDDAAAILAAMEENRAAALLRAYAEEVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q67K92</accession>
    <name>Q67K92_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosomal-protein-alanine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197040.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2923"/>
    <dbReference type="EnsemblBacteria" id="BAD41906">
        <property type="protein sequence ID" value="BAD41906"/>
        <property type="gene ID" value="STH2923"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2923"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_3_9"/>
    <dbReference type="OMA" id="GERYLNY"/>
    <dbReference type="OrthoDB" id="1390922at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="157" mass="17775" checksum="949CFFB8C63B3AA9" modified="2004-10-11" version="1">MGVPEIAVHPMTPADLDQVMEVERLSYLTPWSREAFESELLQRYTVYLVARAGDRVVGFAGMHVLWEMAHVTNVAVHPEFRGRGVGERLMRELIRIAYRRGAARMTLEVRVGNAPAQALYRKLGFVTEPGAVRKGYYTDTGEDAIIMWKEPLVEEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KA3</accession>
    <name>Q67KA3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">3-hydroxybutyryl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2912</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; butanoate metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197029.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2912"/>
    <dbReference type="EnsemblBacteria" id="BAD41895">
        <property type="protein sequence ID" value="BAD41895"/>
        <property type="gene ID" value="STH2912"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2912"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_9"/>
    <dbReference type="OMA" id="FYDYSES"/>
    <dbReference type="OrthoDB" id="862072at2"/>
    <dbReference type="UniPathway" id="UPA00863"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019605">
        <property type="term" value="P:butyrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="6">
        <location>
            <begin position="5"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="5">
        <location>
            <begin position="185"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="3">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="293" mass="32103" checksum="2D3B08784FE418F3" modified="2004-10-11" version="1">MIKRVMVVGAGQMGSGIAQVAAQAGLQVWLRDLEPRFVERGLGVIEKNLARDVAKGRRTEEEKAAILGRITGTTELGPAAECDLVIEAIVESLEAKLALFRELDSICPEVTLFASNTSSLPITQLAAVTRRPDRFIGMHFMNPVPVMKLVEVIRGIATSDETYAAIRNLAEAMGKTPVEVNDFPGFVSNRVLMPMLNEAMFCVYEGIAGVEEVDTVMKLGMNHPMGPLTLADFIGLDTCLAILNVLYEGFKDPKYRPCPLLVQMVQAGYLGRKSGRGFYIYDENGATPMKFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KC4</accession>
    <name>Q67KC4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GNAT family acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011197008.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2891"/>
    <dbReference type="EnsemblBacteria" id="BAD41874">
        <property type="protein sequence ID" value="BAD41874"/>
        <property type="gene ID" value="STH2891"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2891"/>
    <dbReference type="eggNOG" id="COG1246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119519_0_0_9"/>
    <dbReference type="OMA" id="IQEFWVA"/>
    <dbReference type="OrthoDB" id="1823496at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="175" mass="19866" checksum="A859161E103CC70A" modified="2004-10-11" version="1">MLFRKAVMADIPAAHEIINGYAAQGLMLRRPLMMLYESVRDFTVAVDDDGQVLGVAALHIMWRDLAEIRSLAVRPGLTRRGVGRGLVEQLLEEARALGLKRIFALTYQPGFFAKCGFEVVPKESLPQKVWKECVYCDKFHNCDEIAMMRLLVPPEELDDEPWEIPLVARPNWVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KH3</accession>
    <name>Q67KH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196959.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2840"/>
    <dbReference type="EnsemblBacteria" id="BAD41825">
        <property type="protein sequence ID" value="BAD41825"/>
        <property type="gene ID" value="STH2840"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2840"/>
    <dbReference type="eggNOG" id="COG0724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_8_9"/>
    <dbReference type="OrthoDB" id="2029304at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="86" mass="9667" checksum="CCB72C106EA52C84" modified="2004-10-11" version="1">MPFSIYVGNLPWSTTPEDLRALFEPYGEVENARIITDRETGRSRGFGFVDMADEEAARRAIAELHNYEYGGRPLTVNEAQARQGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KL8</accession>
    <name>Q67KL8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase ECF-type sigma factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2795"/>
    <dbReference type="EnsemblBacteria" id="BAD41780">
        <property type="protein sequence ID" value="BAD41780"/>
        <property type="gene ID" value="STH2795"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2795"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_9"/>
    <dbReference type="OMA" id="TWMYRIV"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="124"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="188" mass="21073" checksum="93032AA23EEC1679" modified="2004-10-11" version="1">MEGGKGVVEQEWIRRAQGGDAEAFAALVRLHQDGVYGFCLRMTGRREDALDLTSEAFLRAYRSLAGFDVTQPFRPWLLRIAANLCRDHLRARQQRPAPVEDAVLADLPDPAPPPPGQVLAVHDRAAVRQAVAELPEPYRTAVTLHYFNHLSYQEISEVLNIPVGTVGTHLHRAKRLLRKRLVEQEVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KP7</accession>
    <name>Q67KP7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2765"/>
    <dbReference type="EnsemblBacteria" id="BAD41750">
        <property type="protein sequence ID" value="BAD41750"/>
        <property type="gene ID" value="STH2765"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2765"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_657084_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="3">
        <location>
            <begin position="130"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="418" mass="43861" checksum="3364C336390BD0F1" modified="2004-10-11" version="1">MKQSPWSRGGIPVFWRASASSLVTGGIVAVAFLIPSPMARYSLLAAALLVSVLGGLWVGRAAQAGAAAPPDMQLSPPETGSVAVSVSAAAEAAAGLQPHADTHRTGSADRADLARACDGLIIGAQRTQEAVQEVFRLTGRVNDVLNQLHGASRDQLDDLDRTRGLAHQVVAVFEDMIAAARAAREEAARHRQAAEAVQQALGQIGAGMEGIRAATDASARALRDLDAQSGEIGAIVKLIRDVADQTNLLSLNAAIEAARAGEAGRGFAVVASEVRALADRSRNATRQIQELVAKIQAGTAAAMTAMQESQEEVNRGAATVESTRASIVQVLQSFEGLTMTVEGFGERAEATAGEMAELVKAVEEATRLANQNTTMANELAEADWFSRAIREAEQRAGELVAEARRLEVYTRLPSGGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q67KT4</accession>
    <name>Q67KT4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2728"/>
    <dbReference type="EnsemblBacteria" id="BAD41713">
        <property type="protein sequence ID" value="BAD41713"/>
        <property type="gene ID" value="STH2728"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2728"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_9"/>
    <dbReference type="OMA" id="DQMMVRE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="6">
        <location>
            <begin position="143"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="210" mass="23133" checksum="74DCF2A3E0ABD2DA" modified="2004-10-11" version="1">MIRLLIVDDHKMVREGLKIYLSTEPDIVVVGEAENGEEAARLTAELKPDVILMDLIMPRVDGIEGTKRCLEAHPGVRVIVLTSMPDDELVVPAIRAGALSYVLKDIAADELAETIRKAVSGQPTLHPVAAQRMMQELTAPPKASAGVEEITPRELEVLKLIAQGLSNKEIGERLFIGERTVKTHVSHLLEKLQLQDRTQLAIYALQNKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LB5</accession>
    <name>Q67LB5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196669.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2546"/>
    <dbReference type="EnsemblBacteria" id="BAD41531">
        <property type="protein sequence ID" value="BAD41531"/>
        <property type="gene ID" value="STH2546"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2546"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046586_2_2_9"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="47"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="268" mass="28935" checksum="83AE533856BB9236" modified="2004-10-11" version="1">MDGEKARLAMGYSRTSALYDLLAGHGYLAAIRRLLPLVRVRHRPAILDVGCGTGLNLFEAARWFAPTGPLVGIDLSPGMVAVAAAKARQLGIPATILLGDAERLPLPDASFDLVLCNSVFHWFRDRPAAMREMARVLKPGGQLALITATAPGFREWFLLIDAVIRVVLGPDRAPPIPELPTAEEVAALMQAAGLAVERLQNRIQRDLIVQPLPFVQLMSVIAPTWPGDLSDAEVARVQQAAAQLMAVAWPQGFPFTWSAVEALATKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC3</accession>
    <name>Q67LC3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2538</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2538"/>
    <dbReference type="EnsemblBacteria" id="BAD41523">
        <property type="protein sequence ID" value="BAD41523"/>
        <property type="gene ID" value="STH2538"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2538"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_1_9"/>
    <dbReference type="OMA" id="DTTETIH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="61"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="236" mass="25618" checksum="3C65DA79E28DF371" modified="2004-10-11" version="1">MERALDVLLCFAGERGGLGVTQIAEKLGLYKSTVHRILAALESRGFVRRDPATGRYHLGLRALELAQVYLSSGDLPTIALGEMLQLRDLAQETVSLYVRDGAERVRVQRAEGPLTVRRVVGLGERLPLYLGASGKVLLAWCPPEERARILDAQLPAGFDRTALEARLAEAREQGWALSLEEREEGVASVAAPVIDRAGRCVAALAISGPVSRFTDDRVAMFSRAVQATARSIGMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LC9</accession>
    <name>Q67LC9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="2">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="2">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sucD</name>
        <name evidence="7" type="ordered locus">STH2532</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196655.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2532"/>
    <dbReference type="EnsemblBacteria" id="BAD41517">
        <property type="protein sequence ID" value="BAD41517"/>
        <property type="gene ID" value="STH2532"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2532"/>
    <dbReference type="eggNOG" id="COG0074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_9"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="OrthoDB" id="1081709at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2 3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="290" mass="30467" checksum="FE5B66BD3128705E" modified="2004-10-11" version="1">MAVIIDRNTRAIVQGITGSQGQFHTGQMLAFGTNIVGGVNFKKAGQQVQGVPVFATVHEAVEKTGANASVMFVPAPGVKDAAFEAIHAGIKVLVLIPEHVPTQETMEIMAYAKRHGVRVIGPNTFGIISPGEKCKLGIMPNHIYKPGPVGIVARSGTLSYEIAFNLSNAGLGQTTVIGMGGDRVIGTNFVDALELFNADPATEAIVMVGEIGGIQEELAAEYIKQHVRKPVVAYLAGKSAPPGKRMGHAGAIIEGNRGTYQSKVEALTAAGVKVAALPWEIVDLVREALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LH1</accession>
    <name>Q67LH1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycerate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycerate kinase type-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196613.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2490"/>
    <dbReference type="EnsemblBacteria" id="BAD41475">
        <property type="protein sequence ID" value="BAD41475"/>
        <property type="gene ID" value="STH2490"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2490"/>
    <dbReference type="eggNOG" id="COG1929">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028255_0_1_9"/>
    <dbReference type="OMA" id="YTAVHEK"/>
    <dbReference type="OrthoDB" id="1292731at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031388">
        <property type="term" value="P:organic acid phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018193">
        <property type="entry name" value="Glyc_kinase_flavodox-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004381">
        <property type="entry name" value="Glycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018197">
        <property type="entry name" value="Glycerate_kinase_RE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036129">
        <property type="entry name" value="Glycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21599">
        <property type="entry name" value="PTHR21599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02595">
        <property type="entry name" value="Gly_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006078">
        <property type="entry name" value="GlxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110738">
        <property type="entry name" value="SSF110738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00045">
        <property type="entry name" value="TIGR00045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="381" mass="38614" checksum="F6D13472F080A69D" modified="2004-10-11" version="1">MKIIVAPDSFKGSLTALKAAEAMRQGVLDAMPDAEVVALPMADGGEGTTQALVAATGGQLVEAEVTGPLGEPVRAAFGLLGDGDTAVIEMAAASGLLLVPPERRDPKVTTTYGTGELIRAALDRGVRRILVGIGGSATNDGGVGMAQALGGRFLKADGSPVDRGGAALLELERIDLSGLDPRLKGVELMVACDVDNPLTGPRGAAAVYGPQKGATAEDVALLDRALTRLADVMVRDLGRDVRNLPGAGAAGGLGAGLMGFLGARLRPGIDVVMEVTGLRRQLQGAALLLTGEGRTDGQTLAGKVPLGLARAAAPCGVPVVVISGAVTDDADALLEQGVAALLSTAPGPVTLDEAMARADQWLRRATAQAMRLVRVGIQVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LJ1</accession>
    <name>Q67LJ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196593.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2470"/>
    <dbReference type="EnsemblBacteria" id="BAD41455">
        <property type="protein sequence ID" value="BAD41455"/>
        <property type="gene ID" value="STH2470"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2470"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1694627_0_0_9"/>
    <dbReference type="OrthoDB" id="1526822at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="154" mass="16389" checksum="989AB6278B62C012" modified="2004-10-11" version="1">MYQYRHGHAGDLPWLLQAAAAAAWESLDPEERARVHPLQAAQRAALQCQQVLAAPGSVLLVAQHGAAPVGYLLLALAQDGSTEEPTALLIDLWVHPAHRRRGVGSALLAAAERGVAALGLRKIKLWTGLHHTPVVEFALSRGFVPAGLIGVKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LK4</accession>
    <name>Q67LK4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sec-independent protein translocase protein TatA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tatA</name>
        <name evidence="8" type="ordered locus">STH2457</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms a complex with TatC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TatA/E family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196580.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2457"/>
    <dbReference type="EnsemblBacteria" id="BAD41442">
        <property type="protein sequence ID" value="BAD41442"/>
        <property type="gene ID" value="STH2457"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2457"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_6_2_9"/>
    <dbReference type="OMA" id="WQHILIV"/>
    <dbReference type="OrthoDB" id="1907955at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00236">
        <property type="entry name" value="TatA_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="60" mass="6716" checksum="76593439395BC5BF" modified="2004-10-11" version="1">MFGRLGTMEILLIVLVILLLFGGAKLPQLARGLGDSIKEFRKAVKQDDDKNVSDKEKEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LL0</accession>
    <name>Q67LL0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2451"/>
    <dbReference type="EnsemblBacteria" id="BAD41436">
        <property type="protein sequence ID" value="BAD41436"/>
        <property type="gene ID" value="STH2451"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2451"/>
    <dbReference type="HOGENOM" id="CLU_1895132_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="134" mass="13914" checksum="0C93F04A385871BA" modified="2004-10-11" version="1">MRRAAIPGPARARRRPRASYERHPTRSQKGGIGTVRVRQRLFSLMLALVLAVGAVTVSGCMPQQQPESQQQHSQGGGEDREKQEREEGEKDQEEKETGGGKGGKSGGSGGSGQGGGQQQGGSGGGSQDSTGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LQ7</accession>
    <name>Q67LQ7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2404"/>
    <dbReference type="EnsemblBacteria" id="BAD41389">
        <property type="protein sequence ID" value="BAD41389"/>
        <property type="gene ID" value="STH2404"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2404"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_2_9"/>
    <dbReference type="OMA" id="QVGVAPK"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="112" mass="12233" checksum="574CB470E8C39E2E" modified="2004-10-11" version="1">MAGDKVVTLSASNWAAEVEQSSLPVLVDFWAVWCGPCRMIAPVVEEIAADYEGRMKVGKLNVDENNDLAARYGVMSIPTLILFKNGEPVERIVGFQPKKELAAKIDRALAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LT2</accession>
    <name>Q67LT2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2379</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2379"/>
    <dbReference type="EnsemblBacteria" id="BAD41364">
        <property type="protein sequence ID" value="BAD41364"/>
        <property type="gene ID" value="STH2379"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2379"/>
    <dbReference type="HOGENOM" id="CLU_2439681_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="90" mass="10140" checksum="41DBD47EAD432969" modified="2004-10-11" version="1">MQHAVLQSRCGWRSMAMHEDSADQHFHGEMTVARHRVLLVVAAILVGCSRPTDTVTSTPSPRPSSLRRSRRHLQTPLRIPTLCAQTLIWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LU5</accession>
    <name>Q67LU5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mutator MutT protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mutT</name>
        <name evidence="4" type="ordered locus">STH2366</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196489.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2366"/>
    <dbReference type="EnsemblBacteria" id="BAD41351">
        <property type="protein sequence ID" value="BAD41351"/>
        <property type="gene ID" value="STH2366"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2366"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082381_1_0_9"/>
    <dbReference type="OMA" id="TLPGGWC"/>
    <dbReference type="OrthoDB" id="1308416at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="68"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="208" mass="23875" checksum="6A43E53006D0A3F1" modified="2004-10-11" version="1">MSQNPLWLQWAQQIQSIAQAGITYTKDPYDRERFEQLRAIAGEILARYTSIPDDILSQELAAEQGYLTPKVDVRAVVFNPRGELLLVRERKEGLWSLPGGWADVGESPAEAAVREVREESGYEVRPTKMLAVYDRARHDHPPLIWYVYKLFIRCELVAGTPGNSLETDQVGFFDRDHLPPLSTSRVTEAQIRRMFQHYDNPDLPTDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67LX3</accession>
    <name>Q67LX3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribulose-5-phosphate 3-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2338</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2338"/>
    <dbReference type="EnsemblBacteria" id="BAD41323">
        <property type="protein sequence ID" value="BAD41323"/>
        <property type="gene ID" value="STH2338"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2338"/>
    <dbReference type="eggNOG" id="COG0036">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_9"/>
    <dbReference type="OMA" id="WDGACGA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016857">
        <property type="term" value="F:racemase and epimerase activity, acting on carbohydrates and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="213" mass="23145" checksum="206352BB72F617FC" modified="2004-10-11" version="1">MCAHFGNLAHEVAELEAAGIDMFHLDVMDGRFVPNFGMGLQDIEFIAAHATKPADVHLMIEDPGNYVEKFAKLGIKVIYTHPEADYHPARTLLKIADAGAAPGIAINPGTPVEAIEPLLHLAQYVLVMTVNPGFAGQPYLSFVDEKVDRLAELKERYGYRIVIDGACTAAVVERLAPRGVDGFVLGTKGLFGRGKPYGEVIAELRSRSGDCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M05</accession>
    <name>Q67M05_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA_23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2306"/>
    <dbReference type="EnsemblBacteria" id="BAD41291">
        <property type="protein sequence ID" value="BAD41291"/>
        <property type="gene ID" value="STH2306"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2306"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_271461_0_0_9"/>
    <dbReference type="OMA" id="YLTHHRH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_23" evidence="3">
        <location>
            <begin position="11"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="208"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="501"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="690"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1220" mass="135006" checksum="573A8483B13D5265" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M08</accession>
    <name>Q67M08_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative gluconate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196426.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2303"/>
    <dbReference type="EnsemblBacteria" id="BAD41288">
        <property type="protein sequence ID" value="BAD41288"/>
        <property type="gene ID" value="STH2303"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2303"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_9"/>
    <dbReference type="OMA" id="GMCADWG"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="254" mass="26966" checksum="074902EEE0695182" modified="2004-10-11" version="1">MQLFSLEGRVALVTGAGRGIGRALALGLADAGADVVCLARTGSEVEAAAEEVRARGRRALAVTADVTSQAQVTEAVEAALDRFGKIDILVNNAGINIRKPALEVAEADWDRVVQTNLKGPFLVAQTVGRHMCERGYGRIINVASVGGAVALRTGVAYGASKAGLMHMTRILAMEWARYGVTVNGIGPWYFRTPLTEKLLQDEQYVAEILARTPMRRIGDLAELVGPVVFLASDASSYVTGQVLMVDGGMSVYGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-19" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M29</accession>
    <name>RL251_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L25 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">General stress protein CTC 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplY1</name>
        <name evidence="1" type="synonym">ctc1</name>
        <name type="ordered locus">STH2281</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196404.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67M29"/>
    <dbReference type="SMR" id="Q67M29"/>
    <dbReference type="STRING" id="292459.STH2281"/>
    <dbReference type="EnsemblBacteria" id="BAD41266">
        <property type="protein sequence ID" value="BAD41266"/>
        <property type="gene ID" value="STH2281"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2281"/>
    <dbReference type="eggNOG" id="COG1825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075939_2_1_9"/>
    <dbReference type="OMA" id="HVDFYEV"/>
    <dbReference type="OrthoDB" id="1673077at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000181604" description="50S ribosomal protein L25 1">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <sequence length="205" mass="21816" checksum="A76ADA4A03558BCF" modified="2004-10-11" version="1">MNAVLQLEARTRETGSRASRRLRQAGFVPGIIYGPGVEPLAISVPTIQLERLVDRHGRGHLINAHVEGEANPRQVVIKQLQRDVLTAQVTHVDFLQVDIHRTITLTVPIAVVGEEQAKRRGLIVTHELAEVEIKCRPTEIPEAITLDISGVTEPGPVTVASLAAPPGVEVLEDPDTVVLSCTLGFGGVEEAEAEAEAGSSTGPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M41</accession>
    <name>Q67M41_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2269"/>
    <dbReference type="EnsemblBacteria" id="BAD41254">
        <property type="protein sequence ID" value="BAD41254"/>
        <property type="gene ID" value="STH2269"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2269"/>
    <dbReference type="HOGENOM" id="CLU_2653160_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="76" mass="8924" checksum="BCC570F58C62600F" modified="2004-10-11" version="1">MGLGRSGTQRAPKRRGRERHYFSLDRHGHFSARRSIKQKCNGRWWSRSSRCTRILPWRTPSCSVFARCSVSTKWAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M55</accession>
    <name>Q67M55_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TetR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2255</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2255"/>
    <dbReference type="EnsemblBacteria" id="BAD41240">
        <property type="protein sequence ID" value="BAD41240"/>
        <property type="gene ID" value="STH2255"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2255"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_2_9"/>
    <dbReference type="OMA" id="KLIMAES"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039536">
        <property type="entry name" value="TetR_C_Proteobacteria"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14246">
        <property type="entry name" value="TetR_C_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="215" mass="23462" checksum="50162AF042DE1F94" modified="2004-10-11" version="1">MTTGPASPMSPRPKNPPPDRRAEILDAALRLFAEKGYAEATNAEIAKEAGVTAAALYYYFPSKEELFRTAVRQHIGRFVPTLRALTAQAPADASGPEAVGMILRNALAFFTEEKTQLVLRIVLAEGPRRPEIRQIWIDQITGIFEFLAPFVMNAIASGQIRPIDPRLIFVLLQGPMLSTVIIRDLLQVPALEGLTNDAVVDAVLETTLAGLRTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M88</accession>
    <name>Q67M88_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2220"/>
    <dbReference type="EnsemblBacteria" id="BAD41205">
        <property type="protein sequence ID" value="BAD41205"/>
        <property type="gene ID" value="STH2220"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2220"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2994984_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="57" mass="5836" checksum="7EB000405E1D3F8E" modified="2004-10-11" version="1">MAGGIALGLLSTLAMVLLKPMGVSTVFPSSLAIALKPLFPGFVEANAYFQTIPLSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M91</accession>
    <name>Q67M91_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41202">
        <property type="protein sequence ID" value="BAD41202"/>
        <property type="gene ID" value="STH2217"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2217"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018574_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="514" mass="54299" checksum="801AAAD0C002D315" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M98</accession>
    <name>Q67M98_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH2210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2210"/>
    <dbReference type="EnsemblBacteria" id="BAD41195">
        <property type="protein sequence ID" value="BAD41195"/>
        <property type="gene ID" value="STH2210"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2210"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_9"/>
    <dbReference type="OMA" id="YHRYFEG"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD41195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="323" mass="34922" checksum="8C695711EB2BB3C4" modified="2004-10-11" version="1">MIETRGLVKRYGEFTAVDGLDLEVQAGEVFGLLGPNGAGKTTTILMLLGLTEPTEGWIRVLGHDPVRDPIAVKRVTGYLPDNVGFYDDMTGRQNLMYTAELNGIPRREAAAEIDRLLTRVGLAHAADTRVGAYSRGMRQRLGVADVLLKRPRVVIMDEPTLGLDPEGARAFLELIRQLADEGMTVMLSSHLLYQVQEVCDRVAIFVKGQMIACGRVDELADQALAGEPLRLELELAPCSNGLLTALEQLDGVAELQRLGGSGESVRLALVCRKDLRAEVTRAVASHGAAVLHLSQAGRSLDDIYRRYFADGGETGGRSTEGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MI4</accession>
    <name>Q67MI4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196249.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD41109">
        <property type="protein sequence ID" value="BAD41109"/>
        <property type="gene ID" value="STH2124"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2124"/>
    <dbReference type="eggNOG" id="ENOG502ZW3A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108875_0_0_9"/>
    <dbReference type="OMA" id="VFLPMHI"/>
    <dbReference type="OrthoDB" id="1564524at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="179" mass="19089" checksum="63D6B17744D2EACC" modified="2004-10-11" version="1">MNRRPHQITLSAVFLALALILPFFTGQIPQFGQMLLPMHLPVLICGFVCGWREGLLVGFLAPLLRSAAFGMPPLYPTAVAMAFELATYGTVTGLLQARLPRSTASLLASLVAAMLAGRIAWAAATFALTGALTLELFLAAAFLNAWPGIVLQLLLVPPVVAGIARLVQEEPAMRGSPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ5</accession>
    <name>Q67MJ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH2113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2113"/>
    <dbReference type="EnsemblBacteria" id="BAD41098">
        <property type="protein sequence ID" value="BAD41098"/>
        <property type="gene ID" value="STH2113"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2113"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979790_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD41098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="284" mass="31438" checksum="3333FA39212003C7" modified="2004-10-11" version="1">MKAGEAMTDEDLIRQLQQGSQAAVEPLVRRYHRPLVAYFYRLSHDYHLAQDLAQECLFRLVSRADQYRYPLPLKPWIYRIAVNLWRDVRKSAAYRQGAAALPLDRAVVRAEVLGPLILMGLTASVLLLDQRVDERWGASPRGLHGLFLQRWLLTAAYYVLAIGLFIWLAGARAGDVSEVRVFASSLVTGALFSGACHLFHHLSGSPVAGWAAGLAVYFVTLAIATIWCPYDSVYQLWLPFAGLSDATAQELALSKGVYALAGLALLGMSARLLTVPERLIRGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MJ8</accession>
    <name>Q67MJ8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH2110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2110"/>
    <dbReference type="EnsemblBacteria" id="BAD41095">
        <property type="protein sequence ID" value="BAD41095"/>
        <property type="gene ID" value="STH2110"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2110"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_0_2_9"/>
    <dbReference type="OMA" id="MCIGWGD"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="52"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="194"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="335" mass="35540" checksum="8A69D4F4FCD24B1E" modified="2004-10-11" version="1">MARFQVKVPREQTAPPGPVIFRSVGPFRCSRAPGDGRGPPNGGIVPLPLITLLGLLVAVLAVGCSAILIRLSDAPALVLSFYRLAISVALLAVPALARERETLRRLTGRDWLLLAGSAVCLALHFYVWIESLNFTTVASSTVLVTSSPFIVLALGWRLLGERTNRIGLAAVAIGVAGGVIVGWGDFRVSGSALYGDLLAFLGAVTVSGYTIAGRIARQKMSTTLYTTAVYALAAALLWLMMLPRGIPLAPYPPREWALFAALAVIPTILGHNLFNWALRWVSAATVSMCTLGEPVIASALAWILFREAPGWATVVGGILLMAGIALFVRYGAQPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MK2</accession>
    <name>Q67MK2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spore germination protein KA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gerKA1</name>
        <name evidence="4" type="ordered locus">STH2106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GerABKA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2106"/>
    <dbReference type="EnsemblBacteria" id="BAD41091">
        <property type="protein sequence ID" value="BAD41091"/>
        <property type="gene ID" value="STH2106"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2106"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021639_4_1_9"/>
    <dbReference type="OMA" id="CEIAILY"/>
    <dbReference type="OrthoDB" id="576172at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009847">
        <property type="term" value="P:spore germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004995">
        <property type="entry name" value="Spore_Ger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03323">
        <property type="entry name" value="GerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005690">
        <property type="entry name" value="GerBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="457"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD41091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="551" mass="60667" checksum="99C5617ED274D2FF" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ML4</accession>
    <name>Q67ML4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH2094</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196219.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2094"/>
    <dbReference type="EnsemblBacteria" id="BAD41079">
        <property type="protein sequence ID" value="BAD41079"/>
        <property type="gene ID" value="STH2094"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2094"/>
    <dbReference type="eggNOG" id="COG1999">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_2_1_9"/>
    <dbReference type="OrthoDB" id="1994201at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="PTHR12151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="41"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD41079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27739" checksum="170F053B0715452D" modified="2004-10-11" version="1">MVQEAALPRGVRVGLAVSISVVLLSLAGLLALFASARQPALPVFGQVPDFDFVDQEGRPFSDEDLAGRVHLVGFIYTSCPDICPAITAQMRALQTELARLGLTDQVHLVSITVDPEYDTPERLAAYARMFGADTSSWHFLTGRPNHVRTVVEKGFLVGMDRIATDYPIHAGHHQGHDHGTHAHGPDEAAGAGTTHRSDDALGADHGHGTASAVDYRVEHGGRVALVDREGRIRAYHHGNLLDTDEVMAQIRLLLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MQ5</accession>
    <name>Q67MQ5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196179.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2053"/>
    <dbReference type="EnsemblBacteria" id="BAD41038">
        <property type="protein sequence ID" value="BAD41038"/>
        <property type="gene ID" value="STH2053"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2053"/>
    <dbReference type="HOGENOM" id="CLU_2940149_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269276" evidence="1">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="60" mass="6295" checksum="99790F318CBDA92B" modified="2004-10-11" version="1">MKTTRRNALLTLLTGALAALAVAAGGRGLPGARAAARRLARPAQRQAKVEMYGYTEVNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR7</accession>
    <name>Q67MR7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH2041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196167.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2041"/>
    <dbReference type="EnsemblBacteria" id="BAD41026">
        <property type="protein sequence ID" value="BAD41026"/>
        <property type="gene ID" value="STH2041"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2041"/>
    <dbReference type="eggNOG" id="ENOG5032YTV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1784078_0_0_9"/>
    <dbReference type="OMA" id="HEDNVES"/>
    <dbReference type="OrthoDB" id="1640234at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD41026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="160" mass="17574" checksum="768F8452FDD4869C" modified="2004-10-11" version="1">MISVRGLRPAIWSGLLILMLALLYPRSTRVQTAGAWETTGTFEVPAVAADGTAFSYTMRGHEGQFGFIDSPFVAGKTDKYMWHFWGNQEDFLYKTLEVTGVNRRGQRVPVLTTTLGGEHNGAVAHTPSMMSLPTPGLWRLEVRVDGNHLGDIVVEVLPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MW8</accession>
    <name>Q67MW8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1990"/>
    <dbReference type="EnsemblBacteria" id="BAD40975">
        <property type="protein sequence ID" value="BAD40975"/>
        <property type="gene ID" value="STH1990"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1990"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_9"/>
    <dbReference type="OMA" id="DNTDEQW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0753">Steroid metabolism</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="251" mass="26656" checksum="F0E18CD1822EC364" modified="2004-10-11" version="1">MTAASKGLGVASAMALAREGCDLAICSRNGEAIGAAAERIRQETGRRVLALAADVTRREDLERFITAAMDEYGRVDVVVSNTGGPPPGGFLDFDDAAWEAAFHSLLMPAIRLTRAAIPSMRERGFGRLIYITSSGVKEPIPNLILSNALRAALTNMMKTLAREVAAFGITANTVAPGRIHTDRVDSLDQGEAARTGRTIEEVRRAQEARIPAGRYGRPAEFGQVVAFLASEQAGYITGSAIHVDGGMTVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MX1</accession>
    <name>Q67MX1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Oligoendopeptidase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1987</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="MEROPS" id="M03.A08"/>
    <dbReference type="EnsemblBacteria" id="BAD40972">
        <property type="protein sequence ID" value="BAD40972"/>
        <property type="gene ID" value="STH1987"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1987"/>
    <dbReference type="eggNOG" id="COG1164">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021290_3_1_9"/>
    <dbReference type="OMA" id="HPYMWAV"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09607">
        <property type="entry name" value="M3B_PepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034006">
        <property type="entry name" value="M3B_PepF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011977">
        <property type="entry name" value="Pept_M3B_clade3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02290">
        <property type="entry name" value="M3_fam_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="8">
        <location>
            <begin position="117"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="7">
        <location>
            <begin position="206"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="599" mass="67261" checksum="87979ED485BD2CF6" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N06</accession>
    <name>AROE_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">STH1952</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196079.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N06"/>
    <dbReference type="SMR" id="Q67N06"/>
    <dbReference type="STRING" id="292459.STH1952"/>
    <dbReference type="EnsemblBacteria" id="BAD40937">
        <property type="protein sequence ID" value="BAD40937"/>
        <property type="gene ID" value="STH1952"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1952"/>
    <dbReference type="eggNOG" id="COG0169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_0_1_9"/>
    <dbReference type="OMA" id="FGNPIKH"/>
    <dbReference type="OrthoDB" id="1054867at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000325174" description="Shikimate dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <sequence length="294" mass="31067" checksum="6E002B860832413C" modified="2004-10-11" version="1">MTKRRSTSRGPGRILGILGHPVAHSASPAMHNAAFAAQGMHAMYGAFDVPPSQLEKAIAGIRALGLLGVNVTIPHKEAVMAYLDDVAPTARQVGAVNTIVNRGGRLIGYNTDGWGFLLSLEERGVRVAGRNAVVLGAGGAARAVALHLGMAGVARLTIINRSRQRAEFLAADLARAKTPVRAEVADPGSEEARAALAEAGLVVNCTPLGMEPDTESTPLEDVGLLPAHCVVYDTVYRPLETRLLREARQHGLITVNGLAMLVHQGACAWEYWFGRRGPVDVMRTAALAALEGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N23</accession>
    <name>Q67N23_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1935</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1935"/>
    <dbReference type="EnsemblBacteria" id="BAD40920">
        <property type="protein sequence ID" value="BAD40920"/>
        <property type="gene ID" value="STH1935"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1935"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_8_9"/>
    <dbReference type="OMA" id="KVYFEEW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269456" evidence="2">
        <location>
            <begin position="34"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="94"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="36157" checksum="AC4E2840CE7A3E9C" modified="2004-10-11" version="1">MRKQRLGALLISALLAALLAACSGGSPGDGSGAAGGQAGGQQASVGQSTGEQTTGEPATGEQTGGQQAGGETAYPITLVDGAGREVTIDAEPQRILSLGPSATETLFALGRGDRLVGRTDWCNFPEAALEVPSVGSLFPPDYERILSTEPDLVVMIEGSVDVRERLEQEYGLTVFVYAPASFEQLYAQMVALGQAVNAREAAEAIVAEIQGEVDAIYAKVLPISYSPSVFYQVWPDPLSTAGPGSFIDDMIRIAGGSNVAGDADGPWPVYSLEQLLAADPAIIVAGSEAMAQDILTRPGWESLRAVREGRVYGVPNEDIVVRPGPRLVEGLRWFAETLHPGLFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N38</accession>
    <name>Q67N38_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable glycine dehydrogenase (decarboxylating) subunit 1</fullName>
            <ecNumber evidence="4">1.4.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glycine cleavage system P-protein subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Glycine decarboxylase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Glycine dehydrogenase (aminomethyl-transferring) subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gcvPA</name>
        <name evidence="5" type="ordered locus">STH1920</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-N(6)-lipoyl-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = (R)-N(6)-(S(8)-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GcvP family. N-terminal subunit subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="4"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196047.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1920"/>
    <dbReference type="EnsemblBacteria" id="BAD40905">
        <property type="protein sequence ID" value="BAD40905"/>
        <property type="gene ID" value="STH1920"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1920"/>
    <dbReference type="eggNOG" id="COG0403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004620_0_2_9"/>
    <dbReference type="OMA" id="MYDGASA"/>
    <dbReference type="OrthoDB" id="870147at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00712">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023010">
        <property type="entry name" value="GcvPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42806">
        <property type="entry name" value="PTHR42806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006815">
        <property type="entry name" value="GcvPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="458" mass="49072" checksum="C8A76E2F8061757B" modified="2004-10-11" version="1">MRYVPITQADKAAMLREIGVSSTEELFAANIPAEVRLNRPLNLPPALTEMELLAHLRELAAQNAHTGEYACFLGGGAYDHYVPAAVDAVIRRGEFLTAYTPYQPEISQGVLQAIYEYQSLMCELTGMDLSNASMYDGASALAEAAGMVVNQTGRSKLLVARSVSPLYRRVVQTYMHGIGVELIEVPLDGVTLDLNRLEALMTEEVGGLLLQTPNYLGYLEKVREIEALVHAKNGLFVVAVDPISLGLLEAPGSYGADIVVAEGQGLGVPLSYGGPYLGVLATRDRFARRMPGRIAGATVDNRGQRGFVLTMQAREQHIRREKATSNICTNQALIALAATVYLALVGKEGLREVANLSLQKAHYAAQQIARLPGWRLAGDAPFFKEFTVLAPAAAAEVSRRLVDRKILGGLDVGADYPELLGGAAGSAGGPAAALSFAVTEKRTRAEIDRLVAALGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NC8</accession>
    <name>Q67NC8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">STH1830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1830"/>
    <dbReference type="EnsemblBacteria" id="BAD40815">
        <property type="protein sequence ID" value="BAD40815"/>
        <property type="gene ID" value="STH1830"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1830"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_0_0_9"/>
    <dbReference type="OMA" id="AADQFCE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016211">
        <property type="entry name" value="Glu/Phe/Leu/Val_DH_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42722">
        <property type="entry name" value="PTHR42722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000188">
        <property type="entry name" value="Phe_leu_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="5">
        <location>
            <begin position="144"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="180"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000188-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="BAD40815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="357" mass="39097" checksum="700CD2439DDF10E2" modified="2004-10-11" version="1">MGVFERMMRDGHEQVVFCYDKATGLRAIIAIHDTTLGPALGGCRMWPYATEEEALEDALRLARGMTYKSAASGQNHGGGKVVIWGDPARDKSEPLFRALGRFVGTLRGRIITGTDVGTDKTDFVWARQESPWFVGLPEDEGGSGDTAVLTAYGVWQGMRACARFLWGESSLRNRRIALQGLGKVGSRLLAHLLEDGARVTVTDLRPERVEAVCARHPEVVGVEPDAIYDVPCDIFSPSALGGVLNDATIPRLRCAAVAGSANNQLAEARHGDLLHARGILYAPDYVINAGGLIQVADEILGFNPERARRKTAAIHDLLLRIFAISRDERIPTYQAADRLAEQRIDRIGRRRGIYIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q67ND9</accession>
    <name>Q67ND9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195947.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD40804">
        <property type="protein sequence ID" value="BAD40804"/>
        <property type="gene ID" value="STH1819"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1819"/>
    <dbReference type="HOGENOM" id="CLU_2686481_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="74" mass="8209" checksum="6DCDDF2A0CE1FDC1" modified="2004-10-11" version="1">MREPVTLSREEAVAATLDQLGNRNLQMVDATYTGEYEWRGNRCRAWALRLSTDIGEEVTVTVAVHACTGDLLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI0</accession>
    <name>Q67NI0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195906.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1778"/>
    <dbReference type="EnsemblBacteria" id="BAD40763">
        <property type="protein sequence ID" value="BAD40763"/>
        <property type="gene ID" value="STH1778"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1778"/>
    <dbReference type="eggNOG" id="ENOG5031J4X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125481_0_0_9"/>
    <dbReference type="OMA" id="RYPEVCS"/>
    <dbReference type="OrthoDB" id="1530349at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="171" mass="19766" checksum="C137F5E9517EAEEC" modified="2004-10-11" version="1">MNKRRGDEPSARTIQLLVSYAIRYPEIATVRYDPGRQALRLSFLVKGPLTDEEFSAAAGRIRETLEVYHLMNQREADLIEVRRTAFGELNTIAVERDVHSLSPEELYTVVAVVRSLFQQRLLTEPMEYFGEEELMAQDELIEEVMASLSNQRQQPNLIAIRENGRLMIFHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NI5</accession>
    <name>Q67NI5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1773"/>
    <dbReference type="EnsemblBacteria" id="BAD40758">
        <property type="protein sequence ID" value="BAD40758"/>
        <property type="gene ID" value="STH1773"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1773"/>
    <dbReference type="eggNOG" id="COG0613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067347_1_0_9"/>
    <dbReference type="OMA" id="CTWGGAT"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="249" mass="26915" checksum="E665425CB62B3210" modified="2004-10-11" version="1">MGITDHDTIAGWAEAKEAAEEAGIELVPGCELSTEVGGTEVHILAYYFDPEDPGMADLLRRMRGGRRKRVEEAVAKLHALGYADVSLERILARGGESVGRPHIAAELVDLGIVRTLKEAFERFLAQGRPAYVPRPKLEPAEAIAVVRQAGGVPVLAHPGLIGDDRWVREAIAAGVMGIEAYHTDHSELQRQFYARWGHDAGLVVTGGTDSHGPQGTRTVVPGTVNVGMDVVERLKALSLWYQRSRGSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NM6</accession>
    <name>Q67NM6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome C biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195860.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1732"/>
    <dbReference type="EnsemblBacteria" id="BAD40717">
        <property type="protein sequence ID" value="BAD40717"/>
        <property type="gene ID" value="STH1732"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1732"/>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053225_2_0_9"/>
    <dbReference type="OMA" id="LYFDWMT"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="DsbD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="234" mass="24497" checksum="4638B497D3FA1E4A" modified="2004-10-11" version="1">MASPNLFLAFGAGVLSFVSPCVLPIYPSFLSYLTGVSVDQLSEGSRAVRAHVLKHSLAFFAGFSVIYVALGLTASALGSFFYSSRSWLPVVGGIFIALMGLSMMGILRVPFLMRDTRRQLASRPEGYLGSALIGLTFAAGWTPCIGPILGAVLALAATSPGYGGLLLLAYSVGFALPFIGMAYALGSVRSLARHSVAIQRVGGAVMVFMGILLATGGLERISAWLLEITGFTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NR7</accession>
    <name>Q67NR7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome D ubiquinol oxidase subunit II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH1691</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome ubiquinol oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1691"/>
    <dbReference type="EnsemblBacteria" id="BAD40676">
        <property type="protein sequence ID" value="BAD40676"/>
        <property type="gene ID" value="STH1691"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1691"/>
    <dbReference type="eggNOG" id="COG1294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049294_1_1_9"/>
    <dbReference type="OMA" id="LQFAFAW"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003317">
        <property type="entry name" value="Cyt-d_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43141">
        <property type="entry name" value="PTHR43141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02322">
        <property type="entry name" value="Cyt_bd_oxida_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD40676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="36488" checksum="4FA5182D3658073E" modified="2004-10-11" version="1">MTKGGGHAMEPWLGLDPVAGVAGVMLLALMAYAVLGGADFGGGIWDLLATGPRREQHRQAIAKAMGPVWEANHVWLIFVIVILFTVFPTGYRALGIAFFGLFHLVLVGIVLRGAAFAFRAGASVDSRQWRVWSPVFGGASAVTPFLLGLAAAAVSGGGIRVGAGGVVQVDPGRTWFSPVSVMMGFLTVAMCAYLAAVFLTVETRGDLQEDFRRRALGSGVVLALLAAALLPLLPTGMPQLWAHFSQPRAGPLLLAGAALAVLSAAAIYTRRYLVARVSAVGQVILLLTGWALGQWPYLIYPDVTFHGAAASGPSARFVLQTLPFGLLVLLPSLWLLFRVFKTAVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NS7</accession>
    <name>GPDA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="1">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpsA</name>
        <name type="ordered locus">STH1681</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67NS7"/>
    <dbReference type="SMR" id="Q67NS7"/>
    <dbReference type="STRING" id="292459.STH1681"/>
    <dbReference type="EnsemblBacteria" id="BAD40666">
        <property type="protein sequence ID" value="BAD40666"/>
        <property type="gene ID" value="STH1681"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1681"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033449_0_2_9"/>
    <dbReference type="OMA" id="NRMFGNM"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138044" description="Glycerol-3-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <sequence length="342" mass="35969" checksum="BF41913E0E237341" modified="2004-10-11" version="1">MDMAILPAGVWGTALAVPASAAGRRVRLWRRTPGWAESWDRGHPVLPGLKLPENVVACDSAQAAVSDADLVILSPAGAGLRPVCRLVRPHLRPDAVIVCATKSIEPETHLLVHQVVEEELPGHRGRIVALSGPNFAHEVAAGLPTGAVAACPDLSLADWVQQALMTDRFRVYTNPDLVGVELAGALKNVIALGAGISDGLGMGDNARAALITRGLVEMARLGRAMGANPLTFAGLAGMGDLVLSCTGDSSRNRRAGLAIGRGQSAEAFLAETGLTVEGITTARAGWQLAQRLGVRMPITEAIYQVLYEGLPPLTAMERLMARPRAHELEEVAGADLKPPFDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NV2</accession>
    <name>Q67NV2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na+/H+ antiporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH1656</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CPA3 antiporters (TC 2.A.63) subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195785.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1656"/>
    <dbReference type="EnsemblBacteria" id="BAD40641">
        <property type="protein sequence ID" value="BAD40641"/>
        <property type="gene ID" value="STH1656"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1656"/>
    <dbReference type="eggNOG" id="COG2111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101659_1_1_9"/>
    <dbReference type="OMA" id="HPGFLMP"/>
    <dbReference type="OrthoDB" id="1962104at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="MnhB" evidence="9">
        <location>
            <begin position="11"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD40641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="164" mass="17115" checksum="48EB59B5122E0EAE" modified="2004-10-11" version="1">MVRAQIEQNPIVRVVVHTSLYGLKLLAVWILLRGHHEPGGGFIAGLLIAAAIAMQGVAFGTDAAQAIFPLPPQYLLGGGLLISFATVLGPALAGHAFMEHSAGVIVLPLLGELHWSTAVLFDIGVFLVVVGTMKTVLLSIATDPVRRRPSAAEGGAPSPDREVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NY1</accession>
    <name>Q67NY1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BlaI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BlaI transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1627"/>
    <dbReference type="EnsemblBacteria" id="BAD40612">
        <property type="protein sequence ID" value="BAD40612"/>
        <property type="gene ID" value="STH1627"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1627"/>
    <dbReference type="eggNOG" id="COG3682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119090_1_2_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005650">
        <property type="entry name" value="BlaI_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34134">
        <property type="entry name" value="PTHR34134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03965">
        <property type="entry name" value="Penicillinase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019455">
        <property type="entry name" value="CopR_AtkY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="15539" checksum="2C7B094E9F1506AF" modified="2004-10-11" version="1">MTHLTGTTGFDLGPLEADIMRLVWEKGEVQVDDIHQALLRDREIAYTTVMTVMSRLAAKGLLTRRKHGRAYLYRAALPREEMAESTLHEWSRRFFGGRILPAVSFLLGSERLTPEEVAELRRLVERLEKGDDGGWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67NZ4</accession>
    <name>Q67NZ4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched chain amino acid ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1614"/>
    <dbReference type="EnsemblBacteria" id="BAD40599">
        <property type="protein sequence ID" value="BAD40599"/>
        <property type="gene ID" value="STH1614"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1614"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_1_9"/>
    <dbReference type="OMA" id="MEFTGAR"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="2"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="37302" checksum="68AE4C677CB087DF" modified="2004-10-11" version="1">MKIGWIGPLSGANKTYGESAKRGVELALEEAGYKVGDLTVELIAGDDRSDSTEAVNLATKMMTQDRVAAIIGPVTSGSAIAVSAIAETYKVPMITATATAPKVTVNDDGKRKPFVFRACFIDPFQGQAAANFASRELGLKRAAVFYDKGNDYTIGLAEAFRETFEANGGEVVAWQSYSSEDTDFSAIMTNVAALKPDVLYLPDYYGRVSLIAKAAKDKGINVPMLGGDGWDSPDLDSATLAGGYVTAHYSEQDQREAVQQFATRFQERYGERPDSFAALGYDAAKLLLGAIERAGSTDGEALRAALQSTQDFPAVGGQLSFDEWGNPIKSAALLQVQADGSYKYITNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P01</accession>
    <name>Q67P01_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">tRNA/rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195736.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1607"/>
    <dbReference type="EnsemblBacteria" id="BAD40592">
        <property type="protein sequence ID" value="BAD40592"/>
        <property type="gene ID" value="STH1607"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1607"/>
    <dbReference type="eggNOG" id="COG0565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_0_1_9"/>
    <dbReference type="OrthoDB" id="1637132at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="257" mass="27808" checksum="54E7DB130A003684" modified="2004-10-11" version="1">MPMQPSPLDRVALVLYQPQDVVNVAAIIRVMSNFGLSDLRLVEPAAFDPHRIAGIAHHTEPLIARTRRFPTLEAALADCGLTLGTTGRPRETERQVLPPRAAAPMILDQARTQRVAILFGPERDGLPNAALDLCHGVITIPTHPFNRSLNLAQAALVIAYELWMAWTQPNDGPAHRPPAAAAPPDATESLATGAEREALFAAVDELLSALYPGGSPGRRSSAAARLRALLLRAQPRREEARALTNLLRHAARTGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P20</accession>
    <name>Q67P20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase I subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nuoC1</name>
        <name evidence="2" type="ordered locus">STH1588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1588"/>
    <dbReference type="EnsemblBacteria" id="BAD40573">
        <property type="protein sequence ID" value="BAD40573"/>
        <property type="gene ID" value="STH1588"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1588"/>
    <dbReference type="eggNOG" id="COG0852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_6_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="SSF143243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex1_30kDa" evidence="1">
        <location>
            <begin position="46"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="200" mass="23151" checksum="BB91A51ECDFEF1B8" modified="2004-10-11" version="1">MASAMHERVAAARERVNHEVIPALIEAFPGKVEQIPPTNDLIPQVYVASDVHEPVARWLKERGFHMLVDITAADYWPKREPRFELVYHLREMPEKDPKGDKGLGMIRVRVKLTEKEPVQSLSHIWEMANPAEREIWDQFGLNIVGHPNLTRILNPDDWEGHPLRRDYPLRGPRALINLELPADENRYHPFVETEPAKEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P39</accession>
    <name>Q67P39_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH1569</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1569"/>
    <dbReference type="EnsemblBacteria" id="BAD40554">
        <property type="protein sequence ID" value="BAD40554"/>
        <property type="gene ID" value="STH1569"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1569"/>
    <dbReference type="eggNOG" id="COG1470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045984_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.90.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035328">
        <property type="entry name" value="DUF3048_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021416">
        <property type="entry name" value="DUF3048_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023158">
        <property type="entry name" value="YerB-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11258">
        <property type="entry name" value="DUF3048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17479">
        <property type="entry name" value="DUF3048_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159774">
        <property type="entry name" value="SSF159774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269581" evidence="2">
        <location>
            <begin position="24"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3048" evidence="3">
        <location>
            <begin position="79"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3048_C" evidence="4">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD40554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="345" mass="37393" checksum="3D98FB36EC97C0BC" modified="2004-10-11" version="1">MMRRYLPIVLIVLLLAGCGTRTATPTEPTTPETPGQSTEKPPVSESVHVPEPEPEPVEPTLTTDPVYLLAPSAATLWGGPVSVVVENSPGARPQAGVNQADLVVETLTEAEITRFFTLFWTKPADKIGPVRSARQGFVDMADAYNTPFAHSGGSAEALAMLRAAWGPRNLDEIYTAGGYFYRTGDREPPHNLYTSTDLLGQAIVDRGIDMTSVPTTARSAAVPTVGDHTAVDVFWHRLNEARWVWEDGRYVRYTDGVLHTDEEGRPITAVNLLFLGVGGVNRGVDLGWTLYLWDGGPATVLVAGHRYEGTWRLEPGGFVLYPPEGGQLPPPGARAHLGAPDHRRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-24" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P80</accession>
    <name>RS15_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsO</name>
        <name type="ordered locus">STH1528</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195658.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67P80"/>
    <dbReference type="SMR" id="Q67P80"/>
    <dbReference type="STRING" id="292459.STH1528"/>
    <dbReference type="EnsemblBacteria" id="BAD40513">
        <property type="protein sequence ID" value="BAD40513"/>
        <property type="gene ID" value="STH1528"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1528"/>
    <dbReference type="eggNOG" id="COG0184">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148518_0_0_9"/>
    <dbReference type="OMA" id="FKTHVKD"/>
    <dbReference type="OrthoDB" id="1990141at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000115564" description="30S ribosomal protein S15">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <sequence length="92" mass="10929" checksum="E328393CE8B5A5CB" modified="2004-10-11" version="1">MPLSQEEKQRIINEFKTHETDTGSPEVQIALLTHRINELTEHLRVHKKDHHSRRGLLKMVGQRRALLVYLNKISPERYAKLIDRLNIRTVIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PB4</accession>
    <name>RRF_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-recycling factor</fullName>
            <shortName evidence="1">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">frr</name>
        <name type="ordered locus">STH1494</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PB4"/>
    <dbReference type="SMR" id="Q67PB4"/>
    <dbReference type="STRING" id="292459.STH1494"/>
    <dbReference type="EnsemblBacteria" id="BAD40479">
        <property type="protein sequence ID" value="BAD40479"/>
        <property type="gene ID" value="STH1494"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1494"/>
    <dbReference type="eggNOG" id="COG0233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073981_2_0_9"/>
    <dbReference type="OMA" id="FNPMNNG"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000167560" description="Ribosome-recycling factor">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <sequence length="185" mass="21214" checksum="2B08B65D2CFB7663" modified="2004-10-11" version="1">MTKEIIKDAEERMKKAVEVFRQELAGMRANRATPALLDKVRVEAYGSEVPVNNVATIEVPDPRTLVIKPWDRSLIKAIERAINASDLGLNPTNDGQVIRLSIPPMTEERRRELVKVVAKRTEEQRVAIRNIRRDANEQIKKLEKDKAVSEDESKRAQDEVQKLTDKYIKEVDQLMAAKEKEIMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PC8</accession>
    <name>Q67PC8_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DprA_WH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH1480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1480"/>
    <dbReference type="EnsemblBacteria" id="BAD40465">
        <property type="protein sequence ID" value="BAD40465"/>
        <property type="gene ID" value="STH1480"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1480"/>
    <dbReference type="eggNOG" id="COG0758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029601_0_3_9"/>
    <dbReference type="OMA" id="LALWFEL"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041614">
        <property type="entry name" value="DprA_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17782">
        <property type="entry name" value="DprA_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DprA_WH" evidence="2">
        <location>
            <begin position="326"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD40465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="386" mass="41559" checksum="1C47BED186A689EB" modified="2004-10-11" version="1">MEGTKPKAGCPRAAGRVGESGMEGALVWLIYQRAPGVGRRRMAQLMRTYPDPEVAWRLGKAELAAVRGFRPDIVEALLAVRKSAEARRDAERELERARRAGLRVLCYGTRGYPERLAEIPVPPPVLYQYGPWEPDGRPVVAIVGTRRPTAYGLAVAEQLGRELAQLGAVVVSGMARGIDTEAHRGALEAGGTSVAVLGGGADVCYPQESAALYRQMRETGAILSEQPPGTAPRPENFPERNRIITGLSHGVILVEAGERSGTLITAAHAIEQDRDLFVVPGPVTSPLSRGLYKYVRDGAGLAVSAAQVLEDLGFLPSAEGPLHFTPRGLTELEQRVLGWMGSAPWWPGDLAESSGLPLPEVQACLTMLEIRSAVRRLPDGQYIRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PV2</accession>
    <name>Q67PV2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1306"/>
    <dbReference type="EnsemblBacteria" id="BAD40291">
        <property type="protein sequence ID" value="BAD40291"/>
        <property type="gene ID" value="STH1306"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1306"/>
    <dbReference type="eggNOG" id="COG1061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004880_0_0_9"/>
    <dbReference type="OMA" id="HATFYKH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="826"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="322"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1120" mass="125371" checksum="88709BAA9B5DEA76" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PY6</accession>
    <name>Q67PY6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195403.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1272"/>
    <dbReference type="EnsemblBacteria" id="BAD40257">
        <property type="protein sequence ID" value="BAD40257"/>
        <property type="gene ID" value="STH1272"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1272"/>
    <dbReference type="eggNOG" id="COG3554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055771_2_1_9"/>
    <dbReference type="OMA" id="FDGCIDI"/>
    <dbReference type="OrthoDB" id="1626014at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009467">
        <property type="entry name" value="Glycolipid-bd_prot_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06475">
        <property type="entry name" value="Glycolipid_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="180" mass="19794" checksum="C650E9DC584F8FC6" modified="2004-10-11" version="1">MRQTLRWAAVDSTETEELVLQTEGGGIVADAVVSGVQGGDGTDVTYHLELDPRWQVLRLSVTEGDREVDLTRDSRGTWRDAGGAVLPELQGCADVDISVTPFTNTLPIRRLQLAEGESAEIRVAYVQVPGLVLRPVRQRYTNLGGGRYRYEALDGGYTAVLSVDESGFVLEYPGRFRRLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q50</accession>
    <name>MRAY_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="1">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mraY</name>
        <name type="ordered locus">STH1208</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195339.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67Q50"/>
    <dbReference type="SMR" id="Q67Q50"/>
    <dbReference type="STRING" id="292459.STH1208"/>
    <dbReference type="EnsemblBacteria" id="BAD40193">
        <property type="protein sequence ID" value="BAD40193"/>
        <property type="gene ID" value="STH1208"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1208"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_0_1_9"/>
    <dbReference type="OMA" id="LMSPLHH"/>
    <dbReference type="OrthoDB" id="1151822at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000108912" description="Phospho-N-acetylmuramoyl-pentapeptide-transferase">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <sequence length="334" mass="35215" checksum="0ADC08D8015D558C" modified="2004-10-11" version="1">MEQTTLLRLAGAGLTALAGALALGPVVIPLMRRLRAGQTIRAEMSARHQAKAGTPTMGGVIFIIPAILATLIFAPRSGDALPRLIIALVLTVGHGLVGFADDYIKVVLKRSLGLRARDKLLAQVGLAAVLGYGAVEVLGLGTAVTLPVLNLTLELGRPLYYLLVLIMVWGTASAVNFADGLDGLLGGLSVITFSFYGLVVALALGQTDMAVLGTALVGGVLGFLHYNRHPARIFMGDVGSFALGGALAALAVLTKTEFLLVIVGAVYVIEVISVILQVLSFRLTGRRIFKMAPLHHHFELLGWSEGQVLRLFWGAGLLFTLLGWLVLPGMLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q86</accession>
    <name>Q67Q86_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195303.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1172"/>
    <dbReference type="EnsemblBacteria" id="BAD40157">
        <property type="protein sequence ID" value="BAD40157"/>
        <property type="gene ID" value="STH1172"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1172"/>
    <dbReference type="eggNOG" id="COG2766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028588_2_0_9"/>
    <dbReference type="OMA" id="AYEDSAR"/>
    <dbReference type="OrthoDB" id="49410at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013153">
        <property type="entry name" value="Prk_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010650">
        <property type="entry name" value="PrkA_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016230">
        <property type="entry name" value="Ser_kinase_PrkA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08298">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06798">
        <property type="entry name" value="PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000549">
        <property type="entry name" value="Ser_prot_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00763">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA_PrkA" evidence="1">
        <location>
            <begin position="22"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD40157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="637" mass="72964" checksum="B1E11177F6B99186" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB1</accession>
    <name>Q67QB1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1147"/>
    <dbReference type="EnsemblBacteria" id="BAD40132">
        <property type="protein sequence ID" value="BAD40132"/>
        <property type="gene ID" value="STH1147"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1147"/>
    <dbReference type="eggNOG" id="COG1178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021838_2_1_9"/>
    <dbReference type="OMA" id="VWAHTAN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="61"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="358"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="559" mass="61330" checksum="4526EC909BF50419" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QB9</accession>
    <name>Q67QB9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug efflux protein variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">STH1139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1139"/>
    <dbReference type="EnsemblBacteria" id="BAD40124">
        <property type="protein sequence ID" value="BAD40124"/>
        <property type="gene ID" value="STH1139"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1139"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1155918_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="97"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD40124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="240" mass="26033" checksum="00ECA8BD31D65143" modified="2004-10-11" version="1">MRLSRRARRGVTGESGDASFRAQSKGRRSIMRRNLHRYYVYTVLSSLAFTWTTWFVFVDSRGGNAGWAESAFHLAILLGEVPTGVVADLLGRRRSMLIGLVLGAASFFGYGLIRDTLTACLMLGITGLSLTFISGADAALLYETAAALGGEDFARRATARASALQMMASPRRRSWPAGWLSATPQPRSSPTAPSTSWPPSWSGGCRSHPLRPRRRGALRGPGRTPSRPWLSCAGTRASWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QC9</accession>
    <name>Q67QC9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mutator family transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH1129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the transposase mutator family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1129"/>
    <dbReference type="EnsemblBacteria" id="BAD40114">
        <property type="protein sequence ID" value="BAD40114"/>
        <property type="gene ID" value="STH1129"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1129"/>
    <dbReference type="eggNOG" id="COG3328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036805_8_0_9"/>
    <dbReference type="OMA" id="MESWESA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001207">
        <property type="entry name" value="Transposase_mutator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33217">
        <property type="entry name" value="PTHR33217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00872">
        <property type="entry name" value="Transposase_mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4 6" id="KW-0815">Transposition</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD40114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="407" mass="46135" checksum="D4CC93763C884CD0" modified="2004-10-11" version="1">MTTFRIALEELLRKTGVDDFDFLREGLRVLAQGLMELEVSQRIGADRYERSAERSTYRNGYRERQWDTRVGTIDLQIPKLRKGSYMPSFLEPRRRAEKALVAVVLEAYVNGVSTRKVDDLVQALGMTGVSKSQVSRLCAELDEVVQAFRNRPLESRYPYVWLDAKYVKVRENGRVCTMALVVAVGVREDGDREVLGLDVGPSEDGAFWTAFLRQLVTRGLKGVLLAISDSHVGLQEAIRTVLSGASWQRSRVHFMRNLLGYVPKHWQSMVAAAVRTIFAQPDQQAARRQLAVVADNLRPQFPRAAQLLEEAEDDILAYMAFPTEHWRQLHSTNPLERLNREIGRRTDVVGIFPNREAFIRLAGAVLIEQQDEWTAAPRRYFSQASMAKLYTSNQSVSGTDFLALSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QE0</accession>
    <name>Q67QE0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">STH1118</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1118"/>
    <dbReference type="EnsemblBacteria" id="BAD40103">
        <property type="protein sequence ID" value="BAD40103"/>
        <property type="gene ID" value="STH1118"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1118"/>
    <dbReference type="eggNOG" id="COG1109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_9_1_9"/>
    <dbReference type="OMA" id="KCSQVMY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular transferase activity, phosphotransferases"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="9">
        <location>
            <begin position="12"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="10">
        <location>
            <begin position="173"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="11">
        <location>
            <begin position="274"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="8">
        <location>
            <begin position="389"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="BAD40103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="477" mass="52285" checksum="74F62CC419AD0880" modified="2004-10-11" version="1">MTQTISPLPEVIFREYDIRGVYGRTITDETARLVGRAFARLARERGFREVLVGRDNRLSSPALARALTEGITREGLDVLDIGQVVTPVLYWAREFYGIDPAVMITASHNPGDENGFKLCLGPGTLYGEAIQDLRRRAGALADASPEAEMDPRGGTGPAAGAGRVRTRDPIPSYCEMIAGRVALHRPLKVVVDAGNGTAGPIAPGLFQALGCEVIPLYCESDPTFPNHHPDPVRPENLQDLIRTVRETGADLGLAFDGDGDRLGVVDDQGQILWGDQLMILFWREILPRYPGATALVEVKCSQALVEEIERLGGRPQFHRTGHSYVKKSLRELGGPFAGEMSGHLFFADGYYGFDDALYAGARLCRLVAESGRPLSALWADVPRYPSTPEIRVDCPEERKAAVVEGVVRHFRDRYEVLTVDGARVLFPGGWGLVRASNTQPVLVLRCEGRSTEDLERVKAEMAEALARFPELGPVSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QK0</accession>
    <name>Q67QK0_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1058"/>
    <dbReference type="EnsemblBacteria" id="BAD40043">
        <property type="protein sequence ID" value="BAD40043"/>
        <property type="gene ID" value="STH1058"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1058"/>
    <dbReference type="HOGENOM" id="CLU_3367780_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="35" mass="3927" checksum="A004B6F94FC2A478" modified="2004-10-11" version="1">MNLSESTKAALILDSTSARDQLNLLWHEVSARLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP6</accession>
    <name>Q67QP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small multi-drug export protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1012</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39997">
        <property type="protein sequence ID" value="BAD39997"/>
        <property type="gene ID" value="STH1012"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1012"/>
    <dbReference type="eggNOG" id="COG2426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075669_1_0_9"/>
    <dbReference type="OMA" id="GIKFRHA"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009577">
        <property type="entry name" value="Sm_multidrug_ex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36007">
        <property type="entry name" value="PTHR36007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06695">
        <property type="entry name" value="Sm_multidrug_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="173" mass="18354" checksum="A5B44ED2E7B0F8DB" modified="2004-10-11" version="1">MFVTRLRRADTANPGGFLVFNWSAALKVMAISAIPFLELRFGIPVGIVSGLDPAVAVALGVLGNVLQVPLIIFIMYMLRRIAQLVPWAARILARIDRAAERHEAKVRRYGWLGLALLIGIPIPGTGLWTGAAVANLMRMPLMLTALSMAAGVAIAGVLVGAVTTGAIAVIDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QP9</accession>
    <name>Q67QP9_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycine-rich RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH1009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195141.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1009"/>
    <dbReference type="EnsemblBacteria" id="BAD39994">
        <property type="protein sequence ID" value="BAD39994"/>
        <property type="gene ID" value="STH1009"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1009"/>
    <dbReference type="eggNOG" id="COG0724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_8_9"/>
    <dbReference type="OrthoDB" id="2029304at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="86" mass="9426" checksum="D715E6FEEEF37A2B" modified="2004-10-11" version="1">MAVVSIFVGNLPWATTDEELEALFAPYGEVKRSKIVLDRETRRSRGFGFVDMEEEAAAGAIEALNGQPYNGRVLTVNLAQSRPARA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV9</accession>
    <name>SFSA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar fermentation stimulation protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sfsA</name>
        <name type="ordered locus">STH949</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SfsA family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QV9"/>
    <dbReference type="SMR" id="Q67QV9"/>
    <dbReference type="STRING" id="292459.STH949"/>
    <dbReference type="EnsemblBacteria" id="BAD39934">
        <property type="protein sequence ID" value="BAD39934"/>
        <property type="gene ID" value="STH949"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH949"/>
    <dbReference type="eggNOG" id="COG1489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052299_1_0_9"/>
    <dbReference type="OMA" id="VTAHCPN"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00095">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005224">
        <property type="entry name" value="SfsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040452">
        <property type="entry name" value="SfsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041465">
        <property type="entry name" value="SfsA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30545">
        <property type="entry name" value="PTHR30545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03749">
        <property type="entry name" value="SfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17746">
        <property type="entry name" value="SfsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00230">
        <property type="entry name" value="sfsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152308" description="Sugar fermentation stimulation protein homolog">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00095"/>
        </source>
    </evidence>
    <sequence length="251" mass="27111" checksum="FE21329E1F922694" modified="2004-10-11" version="1">MKGAEPLAELRLRATFLERPNRFVARVRLEDGREVPVHVASSGRMKELLVPGAPVIVTLQGDASAQPTRPFGGRKTAGRLLMVRTGSTWVSVDTSLPGKLFHQAVVAGSCAPFAGYTEVRPEYRYGGSRIDFLLTAPDLPPCLVEVKSVTSVLPDADGARVARFPDAPTARGARHLDELAGAVREGYRAAVCFITQRDDAQAFGPWDEIDPFFGETLRKVARAGVEIRAFVTHVTPEGAVLGGELPVRLAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-19" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QZ8</accession>
    <name>ISPH_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="1">HMBPP reductase</shortName>
            <ecNumber evidence="1">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispH</name>
        <name type="ordered locus">STH910</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195042.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67QZ8"/>
    <dbReference type="SMR" id="Q67QZ8"/>
    <dbReference type="STRING" id="292459.STH910"/>
    <dbReference type="EnsemblBacteria" id="BAD39895">
        <property type="protein sequence ID" value="BAD39895"/>
        <property type="gene ID" value="STH910"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH910"/>
    <dbReference type="eggNOG" id="COG0761">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027486_0_0_9"/>
    <dbReference type="OMA" id="HNKYVVD"/>
    <dbReference type="OrthoDB" id="1311145at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00097"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00105"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051745">
        <property type="term" value="F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13944">
        <property type="entry name" value="lytB_ispH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00191">
        <property type="entry name" value="IspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003451">
        <property type="entry name" value="LytB/IspH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30426">
        <property type="entry name" value="PTHR30426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02401">
        <property type="entry name" value="LYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00216">
        <property type="entry name" value="ispH_lytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000128876" description="4-hydroxy-3-methylbut-2-enyl diphosphate reductase">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="227"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00191"/>
        </source>
    </evidence>
    <sequence length="295" mass="32616" checksum="7854F96479D22429" modified="2004-10-11" version="1">MEVIKITPRGYCHGVVGAIQLVRRVARDPNVPRPIYVLGQIVHNRHVVEEMEALGVITLDGEDRLSLLEKIDAGTVIFTAHGVSPAVKIRAREKGLYVVDATCPDVTRTHELIAELVSRGYEVIYIGKKGHPEPEGAVGVAPGHVHLVERAEDLEALSFAPGQKLAVTNQTTLSQWDTQALMEQVKARWPQTEIYNEICLATQQRQEAVARMAPEADLVIVVGDRRSNNSNRLVQVAQELAAREAHLVDSVEEIDPAWLKGKRKVAVTSGASTPTHITRAVIEFLEKYNVEVEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R01</accession>
    <name>Q67R01_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH907</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195039.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH907"/>
    <dbReference type="EnsemblBacteria" id="BAD39892">
        <property type="protein sequence ID" value="BAD39892"/>
        <property type="gene ID" value="STH907"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH907"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_1_9"/>
    <dbReference type="OMA" id="RIAINQC"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="122"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="186" mass="21562" checksum="17455B75C701ADB5" modified="2004-10-11" version="1">MSQSDRAALPSDRTAAIDHLMREYGTKVLHLAYSYLKDRHLAEDVAQEVFIKAYRNWENFRGESSAYTWLYRITVNLCRDKARSAWWRRLLPTDDPRAAGTPVEPDTAGDDPEEAVVLSDQRERLLDYVMQLSDAYREVIILYYYHDLTTVEIAEVTGQNENTVKTRLFRARAMLKQMLQKGGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R21</accession>
    <name>Q67R21_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH887"/>
    <dbReference type="EnsemblBacteria" id="BAD39872">
        <property type="protein sequence ID" value="BAD39872"/>
        <property type="gene ID" value="STH887"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH887"/>
    <dbReference type="HOGENOM" id="CLU_456278_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="598" mass="64644" checksum="F2B33795BD4BC31D" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R64</accession>
    <name>Q67R64_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Stage 0 sporulation protein A homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH844</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH844"/>
    <dbReference type="EnsemblBacteria" id="BAD39829">
        <property type="protein sequence ID" value="BAD39829"/>
        <property type="gene ID" value="STH844"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH844"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_9"/>
    <dbReference type="OMA" id="QTCLEIM"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="18"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="135" mass="14581" checksum="C33E98D5CF111568" modified="2004-10-11" version="1">MRSGAGAPRRWRESHMALILVADDDPDIARLVAFHLKYNGYEVTLARDGTEALARARETAPDLILLDWMMPGMDGLECLTALKGDPATRGIPVVLMTARAQQADVQAGIAAGAAAYLVKPFELDHLIRTVKEAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R77</accession>
    <name>Q67R77_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH831</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AfsR/DnrI/RedD regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH831"/>
    <dbReference type="EnsemblBacteria" id="BAD39816">
        <property type="protein sequence ID" value="BAD39816"/>
        <property type="gene ID" value="STH831"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH831"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_287694_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005158">
        <property type="entry name" value="BTAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03704">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01043">
        <property type="entry name" value="BTAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="804"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="804"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="1070" mass="117282" checksum="81BBEDF0EBFE4FB4" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RD4</accession>
    <name>Q67RD4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH774"/>
    <dbReference type="EnsemblBacteria" id="BAD39759">
        <property type="protein sequence ID" value="BAD39759"/>
        <property type="gene ID" value="STH774"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH774"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863125_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269948" evidence="1">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="322" mass="33965" checksum="9A3CE9D905A3AAC4" modified="2004-10-11" version="1">MLRRVAAVLLALLLVGSAAYVNRDRSPETAAAGTPGGPVRLLLAVPAGSGSMALRPVEPDTLADLPGFDPIPVPLGLTVIDTAGLRAVGCLDLPVSDLALAPDGRRLLLWGASWDCTTGCAREGHGVYVVDPARLTQVAHLLPGNVATPRGFSPDGRYAYVEEDTGQGKLRHRVLHLATGRLGAGPVMRGPALRVLDAELLRRDREPLRPVRGHQEGGLQPHRAHAGEEELGLHRDHVAGLQRGGLLRSQDRPFVQLEPHAVADEPYPAVARPHEVLLQARPARDVHASLPELLAGGAGARLPGDHVQDLPGGVVGLHELPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RE5</accession>
    <name>Q67RE5_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH763</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194896.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH763"/>
    <dbReference type="EnsemblBacteria" id="BAD39748">
        <property type="protein sequence ID" value="BAD39748"/>
        <property type="gene ID" value="STH763"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH763"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_0_9"/>
    <dbReference type="OMA" id="ETMALYM"/>
    <dbReference type="OrthoDB" id="1325025at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="92"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="317" mass="33495" checksum="FF6AFB2E55BC11EA" modified="2004-10-11" version="1">MSATTAQHPPASAAAALAPDRRRARQIRKERAIAGLLLAAGLVSVVTTVGIVLTLFTETVQFFSLVSVREFLTDRLWTPTFSPAHFGVAPLVSGTFIIAGGAALVAVPLGLGSAIYLSEYAPDRVRRILKPLLELLAGIPTVVYGFFALTFVTPLLKKIIPDIEVFNALSAFITVGIMITPMICSMSEDAMLAVPRSLREAGFALGATRLEVALKVVLPAAFSGVVSSVILAVSRAIGETMIVALAAGSTPKLTLNPLESIQTMTGYIAQVAQGDLEIGSAAYTSMYAVGMALFLITLAMNLLARAISRRYQEGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RF2</accession>
    <name>Q67RF2_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194889.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH756"/>
    <dbReference type="EnsemblBacteria" id="BAD39741">
        <property type="protein sequence ID" value="BAD39741"/>
        <property type="gene ID" value="STH756"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH756"/>
    <dbReference type="eggNOG" id="ENOG5033E1C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1395690_0_0_9"/>
    <dbReference type="OrthoDB" id="1815372at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269335" evidence="1">
        <location>
            <begin position="28"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="195" mass="20634" checksum="D3B119E67D37DE03" modified="2004-10-11" version="1">MKMRKLHLLLATAATIAAFGVGGATLALLTDQASPSSHEFVAGTLRLDGRRGGGDTVPGPMFYLGGDQGLNPTGEWAPGDTQVRWFEIQNVGTLAARMTKISATMSEGADTTLAEQLFVRVTDEYGLEVAAGRLSDFLDEEGEPFRGPAIELEPGDILTLTFEVSLPLGTTDAFQGLSAESVTFSVSAEQVRNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RL7</accession>
    <name>Q67RL7_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">STH691</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194824.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH691"/>
    <dbReference type="EnsemblBacteria" id="BAD39676">
        <property type="protein sequence ID" value="BAD39676"/>
        <property type="gene ID" value="STH691"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH691"/>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_9"/>
    <dbReference type="OMA" id="YVAVCKE"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="174"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="241"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="BAD39676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="459" mass="48842" checksum="B1930AE0D4BCEBFD" modified="2004-10-11" version="1">MTRRWSLRTLALTAVLAALLLPLLALGGGLVGTVALNAGMVSLAEDEVAAAERAVARLVTERWDELGGPAFAAELGELQSRWSFALEVRGLDDRVRYASPSVADGRLVSSGFRLEPTQRVRLLQRNGEPLGLLVLWVWPQAALSGVGAAARVGFGLALASLLALLVGVLVWTGRAVLRPLRALEAATRAVAEGDLDFALPRSRVQELDALGQAFTAMRDRLQAALERQAALQEERRQLIAAIGHDLRTPLSSVRAFAEGLRDGLAREPAKAAHYADVILRKTAEVERLVEDLFQFSRLELPETRAALREVDLAAFLKATLGAFGAQAEQKGVVLAAEGPDLTAGLDPALLGRALDNLIANALRHTPPGGQIRLTWGRHGGEVCIAVADTGEGIPPEELPHLFTPMHRPDRSRSRRSGGAGLGLAIAARIAALHGGRITCESRVGEGTTFTLILPAAPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RU4</accession>
    <name>Q67RU4_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194747.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH614"/>
    <dbReference type="EnsemblBacteria" id="BAD39599">
        <property type="protein sequence ID" value="BAD39599"/>
        <property type="gene ID" value="STH614"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH614"/>
    <dbReference type="HOGENOM" id="CLU_2119886_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019649">
        <property type="entry name" value="DUF2512"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10710">
        <property type="entry name" value="DUF2512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12284" checksum="197B2F630C682EC8" modified="2004-10-11" version="1">MRHVNTLCLKLAAYILIFALALPAAGHRAFPQSLMMAAVHTLVLWLGDLIVLPRYGRTTALAGDAAALVLGSLLMLRAMGAALRFGGLLAAVGMAVLFEAWFHYHLTENRLLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RX1</accession>
    <name>Q67RX1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaG</name>
        <name evidence="17" type="ordered locus">STH587</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13 14">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194721.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH587"/>
    <dbReference type="EnsemblBacteria" id="BAD39572">
        <property type="protein sequence ID" value="BAD39572"/>
        <property type="gene ID" value="STH587"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH587"/>
    <dbReference type="eggNOG" id="COG0305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013501_3_1_9"/>
    <dbReference type="OMA" id="LMWPIRD"/>
    <dbReference type="OrthoDB" id="1071997at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036185">
        <property type="entry name" value="DNA_heli_DnaB-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019475">
        <property type="entry name" value="DNA_primase_DnaB-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013173">
        <property type="entry name" value="DNA_primase_DnaG_DnaB-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10410">
        <property type="entry name" value="DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08278">
        <property type="entry name" value="DnaG_DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002811">
        <property type="entry name" value="DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00766">
        <property type="entry name" value="DnaG_DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48024">
        <property type="entry name" value="SSF48024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="16">
        <location>
            <begin position="257"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="12 14">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002811-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="BAD39572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="623" mass="70199" checksum="6188CBD3E9192A51" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RZ1</accession>
    <name>Q67RZ1_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH567"/>
    <dbReference type="EnsemblBacteria" id="BAD39552">
        <property type="protein sequence ID" value="BAD39552"/>
        <property type="gene ID" value="STH567"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH567"/>
    <dbReference type="eggNOG" id="COG4243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047686_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="332" mass="35501" checksum="2A48FD72DDB0837F" modified="2004-10-11" version="1">MLRIGLALPSLSPTLSGGHGPLMISGFLGTVIGLERAVAMGRPWMFAAPALAAAGGLLILCGAPAAAGALSMALSSAALILLFTRIVRVQPTLFNYVLTLAAAVWLGGNLSWLFGTPITHVVPAWAGFLVLTIAGERLELARLVRISRGGTAAFLAVLAVVVAGAALTPFHYSLGWRVTGLGLAALSLWLLRYDIARRTVRTRGLTRFIALCMLPGYFWLLVGGLIPLRYGFIYGGIYDAALHAVFLGFVFSMIFGHAPVIFPAVLGVRMAFTPRFYSHLVLLHLSVLLRVGAGLAEWLPGRQWAVVLNVAAVLLFLLNTVRSVRRKPAQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S21</accession>
    <name>Q67S21_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore peptidoglycan hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH537"/>
    <dbReference type="EnsemblBacteria" id="BAD39522">
        <property type="protein sequence ID" value="BAD39522"/>
        <property type="gene ID" value="STH537"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH537"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037415_4_1_9"/>
    <dbReference type="OMA" id="WYEAHDY"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02874">
        <property type="entry name" value="GH18_CFLE_spore_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041704">
        <property type="entry name" value="CFLE_GH18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="47"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="2">
        <location>
            <begin position="99"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="426" mass="47663" checksum="9A0C9DDC46255EF0" modified="2004-10-11" version="1">MVEPGQSLYAIARMYSVTPESIAQANQLNGLPYLVVGQALVIPITGRYYWVQPGDSLWSVGQRFGLDPSELARINGIPTGAMLPVGLRLYIPPAPRPRTEVNAYVEPFGESVSAGLVAASREAAPHLTYLAPFSFRVQQDGTLQAPPLDDLLQIARDAGAVPMLVVTNLEEGGFSDELGRIILNDQAVQDRLLGEIQRLIREMGFRDVHFDFEFLRPQDREAYNRFLRRAADLIHGEGALISTALAPKVSAGQTGQWYEAHDYAAHGQTVDFVVLMTYEWGYSGGPPMAVSPIDQVRRVLEFAVTQIPREKIMMGQNLYGYDWTLPYRPAGEYARAISPQEAIRQAARRGAAIEFDTRAQAPFYRYVDEQGRTHEVWFEDARSIQAKFNLLKELGLRGISYWKLGLDFPQNWLLLEDNFTVAKRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S45</accession>
    <name>Q67S45_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH513</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194647.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH513"/>
    <dbReference type="EnsemblBacteria" id="BAD39498">
        <property type="protein sequence ID" value="BAD39498"/>
        <property type="gene ID" value="STH513"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH513"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_8_1_9"/>
    <dbReference type="OMA" id="SDCLFCK"/>
    <dbReference type="OrthoDB" id="1934382at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="6"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="99"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="114" mass="12265" checksum="89D5EFADB3F1CF04" modified="2004-10-11" version="1">MAHDCVFCKIAAGEIPSSKVYEDEHVLAFRDINPVAPVHVLVIPKQHVPSVAEFGPEHDELLSRLFAAVRKVAAETGVAETGYRVVTNVGKDAGQQVFHVHLHVLGGRPLGPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SD3</accession>
    <name>Q67SD3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4342 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH425"/>
    <dbReference type="EnsemblBacteria" id="BAD39410">
        <property type="protein sequence ID" value="BAD39410"/>
        <property type="gene ID" value="STH425"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH425"/>
    <dbReference type="eggNOG" id="COG1308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115782_2_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025642">
        <property type="entry name" value="DUF4342"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14242">
        <property type="entry name" value="DUF4342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4342" evidence="2">
        <location>
            <begin position="60"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="136" mass="15273" checksum="66199A750B9E7A5A" modified="2004-10-11" version="1">MPEEEGDSMEEREKLEKMDLLRNRFKISYARAREVLEQNGWDAVAAAVQLEEEQTPSGFFTEELKVSGRDLVETLKRILHEGNVNRIIVRDPKGYEILNLPVGGAVVFALVLPMLTALSAVVILALDYTVVVERSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SH3</accession>
    <name>Q67SH3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tungstate ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH385"/>
    <dbReference type="EnsemblBacteria" id="BAD39370">
        <property type="protein sequence ID" value="BAD39370"/>
        <property type="gene ID" value="STH385"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH385"/>
    <dbReference type="eggNOG" id="COG2005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2998">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061511_1_0_9"/>
    <dbReference type="OMA" id="SATMWKE"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="125"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="353" mass="37972" checksum="D7A251CC74A120CF" modified="2004-10-11" version="1">MPADYRPLLARDDQFDRICAILGAVAEQGSLRKATEVLGVSYRYAWGLIRRAEEEIGTPLLVRRVGGASGGGAELTPTAQDLLRRHRLLRREAAAILGPERADAALERPLLMASTIGPVEVGLVDALVGAYRAETGQWVRYIAAGSGQALDLGRAGRVDLALVHAPELEERFLAEGYGTGRYPLAQNDFVVCGPADDPARVRQAASAVDAMRRIAEGRHPFVSRNDQSGTNVRELRLWQLAGISPEPPWYEPWPLGGQGNIATLRHAAAIGAYTLIDSATLVMACPEGYAGLFRGDPALVNQFCLIPVNPELFPFVNGAGARRFAEWATGPAGRAVVRAFGTAAYGEPLFRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SM4</accession>
    <name>Q67SM4_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH334</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH334"/>
    <dbReference type="REBASE" id="10266">
        <property type="entry name" value="M.StpORF334P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="BAD39319">
        <property type="protein sequence ID" value="BAD39319"/>
        <property type="gene ID" value="STH334"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH334"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_0_9"/>
    <dbReference type="OMA" id="DIIWHKP"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="8">
        <location>
            <begin position="36"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="289" mass="33057" checksum="217E044DC8E3AE19" modified="2004-10-11" version="1">MQHERITPPPKLELQGSLILEGDARLALQRIPDNSIQCVVTSPPYWSLRDYGIEGQIGLEDSVYQYINTLVSVFREVRRVLKPDGTLWLNIGDSYTSGGRTWRAPDKKNQARAMSIRPDTPEGLKPKDLIGVPWRLAFALQQDGWYLRSDIIWHKPNAMPESVKDRPTRSHEYIFLFSKSERYYYDYQAIREENGRNRRSVWHINTQPNKEAHFAVFPTTLVEPCILAGSKPGDYVLDPFLGSGTTAVVCQNLDRKYVGIELNPEYIQIAVKRLTSILTEAPQVTKVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SP6</accession>
    <name>Q67SP6_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194447.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH312"/>
    <dbReference type="EnsemblBacteria" id="BAD39297">
        <property type="protein sequence ID" value="BAD39297"/>
        <property type="gene ID" value="STH312"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH312"/>
    <dbReference type="eggNOG" id="COG3649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071770_2_0_9"/>
    <dbReference type="OMA" id="MGRKPIV"/>
    <dbReference type="OrthoDB" id="598606at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006482">
        <property type="entry name" value="Cas7_Csh2/Csh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013418">
        <property type="entry name" value="CRISPR-assoc_prot_Csd2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05107">
        <property type="entry name" value="Cas_Cas7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02589">
        <property type="entry name" value="cas_Csd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01595">
        <property type="entry name" value="cas_CT1132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="370" mass="41383" checksum="AC0687674432E745" modified="2004-10-11" version="1">MSHTDVSKRHEFVLLFDVRDGNPNGDPDAGNLPRIDPETMHGLVTDVALKRKVRDYVSGVLGKPIFIQSKVALNTLILGAFRHVGYEPVVTSLQGEELEDEELMAWLAGKADAGFAVDGERLLYSGESLRQQDIIRALTEGIDEEQRELNRKLRDLGRRLADAAKARKGELTAETQDKARKWLCQTYYDIRMFGAVLSTGLNAGQVRGPVQLTFARSQHPITPLDLSITRQARTTTVRMATGPTEMGRKPIVPYGLYRAHGFFNPFLAEKTGVTDDDLRILWDALQHLFDYDRSAVRGEMNMRGLWVFTHDDAKGCAPTHKLFELIQTDKLRNGVEVPRDFSHYDVQSPPEGPLDALGYPGVTLTCLVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS7</accession>
    <name>Q67SS7_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194416.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH281"/>
    <dbReference type="EnsemblBacteria" id="BAD39266">
        <property type="protein sequence ID" value="BAD39266"/>
        <property type="gene ID" value="STH281"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH281"/>
    <dbReference type="HOGENOM" id="CLU_1304354_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="211" mass="24411" checksum="DC2DA90A61516951" modified="2004-10-11" version="1">MGNDPVERHIRTAFDYVVRVYEELHFLFQDLRTALQERDRNLQPLVGRIYGFQSGMDKPRHWLARYVSECWVDDRHGLMEGRRAAYLSATVSLLTPDDQPIEPLFSYGVVAPMNHPQAMDFCHQGVWYATANHQDLFSYRVDDNEVSGHSLPMNTEIWFRSKLPDGSYAWPKRGVMVIQPLTAIRDTNSVYEIAGKMVSLWNKYADQVARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SX1</accession>
    <name>Q67SX1_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194372.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH237"/>
    <dbReference type="EnsemblBacteria" id="BAD39222">
        <property type="protein sequence ID" value="BAD39222"/>
        <property type="gene ID" value="STH237"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH237"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_2_9"/>
    <dbReference type="OMA" id="GWFFLFE"/>
    <dbReference type="OrthoDB" id="1763210at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004269361" description="Phosphate transport system permease protein PstA" evidence="7">
        <location>
            <begin position="25"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="53"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD39222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="265" mass="26748" checksum="2F75A7DFFBF0CB1B" modified="2004-10-11" version="1">MRPLLSALALAVTGVMVGLLAALAAGGAGSLTPEFLFTLPAAAGREGGILPAILSTLWLAAGSLALALPLGVGTALYLAEYAPPGRWVAAIRSLTETLAGVPSILFGLFGFSVFVVRLGWGPSLLAGSATLALMLLPTVIRTSEEAVAAVPQELREGAAALGATRWDAVRRIVLPQAAPGILTGALLALGRAAGETAAVLLTAGTDLALPRSPLDPGRSMAVHLYLLAGEGLSDGRAHATALALVCGVMVLNALAQLLMGGRRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T20</accession>
    <name>Q67T20_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sugar isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">STH188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH188"/>
    <dbReference type="EnsemblBacteria" id="BAD39173">
        <property type="protein sequence ID" value="BAD39173"/>
        <property type="gene ID" value="STH188"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH188"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637645_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008731">
        <property type="entry name" value="PTS_EIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036618">
        <property type="entry name" value="PtsI_HPr-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05524">
        <property type="entry name" value="PEP-utilisers_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47831">
        <property type="entry name" value="SSF47831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="2">
        <location>
            <begin position="219"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="BAD39173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="430" mass="46349" checksum="3FE6783F777E9796" modified="2004-10-11" version="1">MAVYRAQGVWPGEAEGRIVWVDPEWQPERAGCADRPAEIGRLREAVGRALHDLDAWMDRQPTLGGRVLLQHCREALQDPAFMERVTMLIDQHGLTAPAALMEAASLVGGIMARSEELRERAAALRQTARWLARRLDGAAHPEDAILASRAFSALELLDRTRPAVMAAGEPVVVGDAPLLWGVDAVAPEWAGRPARIRGGTLILGEEDEAGGLLHEAARRAVLAVARRMKAEGLVRMKEGNVSCRIPGTSLFAVTPSGLPYESLEPADICILDLDGRVVDARRRPSTETALHRFVYRRRPDVGGVVHTHSLHATAFACTGREIPVISTELAALVGAEVPCAPYAPAGTERLAEVVAEALGDEGAAALLQNHGVVTVGETVEQAYAAAVAVEVAAQVFLLARQLGEPIVLPPEERRRIFRSMRTGYGQPREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q67T75</accession>
    <name>Q67T75_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Potassium channel TrkA variant</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194268.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH133"/>
    <dbReference type="EnsemblBacteria" id="BAD39118">
        <property type="protein sequence ID" value="BAD39118"/>
        <property type="gene ID" value="STH133"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH133"/>
    <dbReference type="eggNOG" id="COG0490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2132274_0_0_9"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0407">Ion channel</keyword>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="113" mass="12634" checksum="B4DF812D3EA70A22" modified="2004-10-11" version="1">MPGIRETDLPGIGRKFLIETRSGDKMVVVHDDGRRAIYHFDQEDPDEPVSMVTLDDTEARQVAAIVGGLAYQPKVLEEMDMDVNDLMIKWYRLGPHSPGAPSCSSWWSSARSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TA5</accession>
    <name>Q67TA5_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stage III sporulation protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spoIIID</name>
        <name evidence="4" type="ordered locus">STH103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194238.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH103"/>
    <dbReference type="EnsemblBacteria" id="BAD39088">
        <property type="protein sequence ID" value="BAD39088"/>
        <property type="gene ID" value="STH103"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH103"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174628_0_0_9"/>
    <dbReference type="OMA" id="VHKDMTE"/>
    <dbReference type="OrthoDB" id="1848020at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014208">
        <property type="entry name" value="Spore_III_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018356">
        <property type="entry name" value="Tscrpt_reg_HTH_DeoR_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12116">
        <property type="entry name" value="SpoIIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02844">
        <property type="entry name" value="spore_III_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00894">
        <property type="entry name" value="HTH_DEOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="BAD39088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="82" mass="9488" checksum="7F2FD1C00B40CFFC" modified="2004-10-11" version="1">MKDHIWKRVLEIGNHICRTKDTVRETASVFGVSKSTVHKDVTERLPRINPELAAKVKRVLDQNKAERHIRGGAATRQKYLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TD3</accession>
    <name>Q67TD3_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="7">TC-AMP synthase</shortName>
            <ecNumber evidence="4 7">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">STH75</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="4 7"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194210.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH75"/>
    <dbReference type="EnsemblBacteria" id="BAD39060">
        <property type="protein sequence ID" value="BAD39060"/>
        <property type="gene ID" value="STH75"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH75"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_0_0_9"/>
    <dbReference type="OMA" id="GMLKSHY"/>
    <dbReference type="OrthoDB" id="1913202at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="9">
        <location>
            <begin position="16"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="BAD39060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="349" mass="36519" checksum="A561707188B7E1AF" modified="2004-10-11" version="1">MQGTRVLEVDPADPEPEVVAQAAAILRAGDVVAFPTETVYGLGANALSSEAVARIFAAKGRPSDNPLIVHIADTAQLEGLVESVPPTAERLMERCWPGPLTLVMRKGPAVPPEVTAGLDTVGIRMPDHPVALALIRAAGVPIAAPSANASGRPSPTTAEHVLEDLAGSIPAVLDGGETGVGVESTVLDMTTDPPMILRPGGLPREELEAAIGPVVLAPGVEGGTVERPRSPGMKYRHYAPQAPLILVEGPVLEMQEKIRDLAMEYAEEGKRVGILCSAESRGVYVAPVILEYGARGDLAGIASDLFGTLRAFDRHQVDVILAEGVPEEGLGLAIMNRLRRAAGGRVIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q67L31</accession>
    <name>Q67L31_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycine betaine/carnitine/choline ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">STH2630</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196752.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2630"/>
    <dbReference type="EnsemblBacteria" id="BAD41615">
        <property type="protein sequence ID" value="BAD41615"/>
        <property type="gene ID" value="STH2630"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2630"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_2_2_9"/>
    <dbReference type="OMA" id="RMINQLD"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031460">
        <property type="term" value="P:glycine betaine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005892">
        <property type="entry name" value="Gly-betaine_transp_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01186">
        <property type="entry name" value="proV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="255"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="315"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="BAD41615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="376" mass="41958" checksum="B83E9B3053F4700C" modified="2004-10-11" version="1">MVRFEHVSKVYPDGTKAVEDLNLHIARGEFVCLIGPSGCGKTTTLKMINRLHEATSGKIYVDGRDIATVSPVELRRNIGYVIQQVGLFPHMTIAQNIELVPRLLGWDRERRRKRVDELLEMVGLDPATYRDRYPRELSGGQQQRVGVLRALAAEPDLILMDEPFGALDPITRETLQDELKRLQARLRKTVVFVTHDMDEALKLADRIVVMKDGRIHQVASPEELLRNPKDEFVAQFVGRQRMVRPAESLTVADVMIPDPVTAGPEYGIAEAVATMRRRRVNSVLVVDGDGRLLGIVTARAVERGLASHRTLGEIMETRLTTVLPHQPVTHAVQRMLLERLEFLPVVDEQGRLQGLVTNTSLVGTLSRAERAGGATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67M39</accession>
    <name>Q67M39_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase III-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH2271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2271"/>
    <dbReference type="EnsemblBacteria" id="BAD41256">
        <property type="protein sequence ID" value="BAD41256"/>
        <property type="gene ID" value="STH2271"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2271"/>
    <dbReference type="eggNOG" id="COG2176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707732_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="1">
        <location>
            <begin position="23"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD41256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="390" mass="43794" checksum="09279805B00E6D26" modified="2004-10-11" version="1">MAITGAGFLGENGTKGGVDMRYNLVVIDTETTGLEYDQHRIIQIGVVAIKEQRIVKTWESKLRLIDGEVSAYAMQVNRLDPSDMRSEKYPHPKAVLRKLQEEFLRPGVLLGAHNLSFDLGFLQAEFARHGLPQWQGNFVDSMALGRALLPDLSASLESLARHFRVELTQHHDALADAVATAHVLLHLLDLAQQRGLDPLNLLIERQGRPIRYRPDNARVVTQEEILAELAKIQQQRAAATDTASARSASSGGLFSALRAAVRETVQKMAAPKYPLLPSPREALAYARDHQSGSRFVVRGVSGRDVEVVVKYHPIRSSHPQHRHKLQRYQIFYRHKGQLYPYANAHTEKHQMDGRTLYLWEYHDAYQALSEAALRQWLTKVLAGASQSIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q67MR3</accession>
    <name>Q67MR3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">STH2045</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD41030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH2045"/>
    <dbReference type="ESTHER" id="symth-q67mr3">
        <property type="family name" value="CarbLipBact_2"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.946"/>
    <dbReference type="EnsemblBacteria" id="BAD41030">
        <property type="protein sequence ID" value="BAD41030"/>
        <property type="gene ID" value="STH2045"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH2045"/>
    <dbReference type="eggNOG" id="COG1647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076594_0_0_9"/>
    <dbReference type="OMA" id="WLDDSYH"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012354">
        <property type="entry name" value="Esterase_lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017388">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="6">
        <location>
            <begin position="52"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="6">
        <location>
            <begin position="193"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR017388-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR017388-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR017388-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="BAD41030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="280" mass="30212" checksum="8430D164BA42DEF6" modified="2004-10-11" version="1">MSRKSRNCRNPPRSGDAQQRPRERSGSGMSTTPLQVLPGAEPLYSVGSRIGVLVSHGFTGSPQSMRFLAEGFARAGYTVAMPRLTGHGTTPAEMAASTASDWTADIVAAMRWLEERCDVLFMTGLSMGGALTVWAAGQFPERFAGIMPINAALRMESPDLAALAFNPDAPAELPGIGSDIKAEGVKELAYPVTPVPAIKHLITIGAVAEMLLPRVKCPALIIQSREDHVVPPHNGELIYNGIGSTEKELLWLENSYHVATLDNDKELILERSLAFIRKHS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q67N41</accession>
    <name>GLYA_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">STH1917</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011196044.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67N41"/>
    <dbReference type="SMR" id="Q67N41"/>
    <dbReference type="STRING" id="292459.STH1917"/>
    <dbReference type="EnsemblBacteria" id="BAD40902">
        <property type="protein sequence ID" value="BAD40902"/>
        <property type="gene ID" value="STH1917"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1917"/>
    <dbReference type="eggNOG" id="COG0112">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_9"/>
    <dbReference type="OMA" id="SHPAGLI"/>
    <dbReference type="OrthoDB" id="861782at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113680" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="412" mass="45508" checksum="D481E40DFB2B0462" modified="2004-10-11" version="1">MDALKRYDPEVFAAIQQEVERQQRNIELIASENFVPKAVLEAAGTVLTNKYAEGYPGRRYYGGCEYVDIVENIARERLKAAFGAEHVNVQPHSGANANTAVYFAFLQPGDTVLGMNLAQGGHLTHGSPVNFSGRTYNFVPYGLDPETERINMDQVAELARQHRPKLIVAGYSAYPRVLDFKRFREIAEEVGAILMVDMAHFAGLAATGYYPNPVEHAHVVTTTTHKTLRGPRGGAILCKKEFAKEIDKAVFPGMQGGPLMHIIAAKAVAFKQLSDPDYRAYCGQVVKNAKALAQALLERGYRLVTGGTDNHLMLVDLRPKGITGRDAEHLLDRVSITVNKNAIPNDPEKPMVTSGIRIGTPAMTTRGMKEAEMVQIADLIDRAITHRNDEAELDRIRAEVHELTARFPLYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q67P74</accession>
    <name>Q67P74_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH1534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195664.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1534"/>
    <dbReference type="EnsemblBacteria" id="BAD40519">
        <property type="protein sequence ID" value="BAD40519"/>
        <property type="gene ID" value="STH1534"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1534"/>
    <dbReference type="HOGENOM" id="CLU_2866163_0_0_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD40519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="64" mass="7672" checksum="99965ACD12F72D19" modified="2004-10-11" version="1">MDSERETRARIEELRQRLHRQVSGPLTPHQLQGLLPISQEIDRLAVDFIRRRWQQTAVKQAQRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q67PR8</accession>
    <name>RPOZ_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="1">RNAP omega subunit</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoZ</name>
        <name type="ordered locus">STH1340</name>
    </gene>
    <organism>
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195471.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q67PR8"/>
    <dbReference type="SMR" id="Q67PR8"/>
    <dbReference type="STRING" id="292459.STH1340"/>
    <dbReference type="EnsemblBacteria" id="BAD40325">
        <property type="protein sequence ID" value="BAD40325"/>
        <property type="gene ID" value="STH1340"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1340"/>
    <dbReference type="eggNOG" id="COG1758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125406_6_1_9"/>
    <dbReference type="OMA" id="KRARMLT"/>
    <dbReference type="OrthoDB" id="1864134at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000237515" description="DNA-directed RNA polymerase subunit omega">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <sequence length="69" mass="7703" checksum="4DCAFC00D2BBBF36" modified="2004-10-11" version="1">MIKPSLDQLMHLVDSKYTLVILAARRARDLQDGKHQMVESKSNKPVTIALEELAAGKLFFERGKPTPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q67Q08</accession>
    <name>Q67Q08_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">STH1250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">T / IAM 14863</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YajC family.</text>
    </comment>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD40235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH1250"/>
    <dbReference type="EnsemblBacteria" id="BAD40235">
        <property type="protein sequence ID" value="BAD40235"/>
        <property type="gene ID" value="STH1250"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH1250"/>
    <dbReference type="eggNOG" id="COG1862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116157_6_1_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="BAD40235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="104" mass="11464" checksum="94E85FA1A7D8B023" modified="2004-10-11" version="1">MWESILAASIVMLAFVLVTLAIWYALAIRGLRRQREQFARLHAELAPGQVVTFGNGIYGTTVAVYEDTADIQVKSGAVLTVSRYAISAIVRKEKQHAQHLADAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q67QV8</accession>
    <name>Q67QV8_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription antiterminator BglG family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">STH950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011195082.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH950"/>
    <dbReference type="EnsemblBacteria" id="BAD39935">
        <property type="protein sequence ID" value="BAD39935"/>
        <property type="gene ID" value="STH950"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH950"/>
    <dbReference type="eggNOG" id="COG1762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013442_5_2_9"/>
    <dbReference type="OMA" id="TIDAQVF"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007737">
        <property type="entry name" value="Mga_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05043">
        <property type="entry name" value="Mga"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PRD" evidence="4">
        <location>
            <begin position="171"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="PRD" evidence="4">
        <location>
            <begin position="285"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-2" evidence="3">
        <location>
            <begin position="495"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="BAD39935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="642" mass="73930" checksum="B7A41D05E210874E" modified="2004-10-11" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q67R58</accession>
    <name>Q67R58_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194982.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH850"/>
    <dbReference type="EnsemblBacteria" id="BAD39835">
        <property type="protein sequence ID" value="BAD39835"/>
        <property type="gene ID" value="STH850"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH850"/>
    <dbReference type="eggNOG" id="ENOG502ZKAD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2829900_0_0_9"/>
    <dbReference type="OMA" id="FADYRIE"/>
    <dbReference type="OrthoDB" id="2086659at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="67" mass="8010" checksum="42C08AFD201E2BDD" modified="2004-10-11" version="1">MQYLVFEFPSEKELRDTVKKLWDGYNVSGEMAIKPIGNGKWRLEMYTEKELRESTLEKFADYRIEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q67RJ9</accession>
    <name>Q67RJ9_SYMTH</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH709"/>
    <dbReference type="EnsemblBacteria" id="BAD39694">
        <property type="protein sequence ID" value="BAD39694"/>
        <property type="gene ID" value="STH709"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH709"/>
    <dbReference type="eggNOG" id="COG1541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_3_9"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="132"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="391" mass="41424" checksum="C95CC23F491C8E75" modified="2004-10-11" version="1">MRKVLAAARAGSPVWRERLDAAGLADAEPGGDGMPGGPRWQALPVLRKELLGSVQAGSPPFGGLLGVPVEQLARIFVSPGDVYDPQGTDPDYWRFGKVLAAAGFERGERVLNCFSYHLTPAGFMFDAAARALGCVVVPAGVGQQDLQIRVARAVGATGYTGLPSYLLALLDRAAAAGTPLPLRRAVVTGEPLPEALRARIEAHGVTVRQAYGTADVGLIGYECGRGAGLHIDDGVIVEICDPDGRTLPPGEVGEVVVTALQETYPLIRFGTGDLSALVAEPCPCGRPSARLKGWMGRVSEVTKVRGIFLYPKQLEAAMAGLAGGVQRWRAVVERDADHRDVLRIEFAGQADPRAVRDAVRAAVRLNPDVLVVPPGAIPADGPLFQDRRNWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q67S05</accession>
    <name>Q67S05_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alanine racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">STH553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194687.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH553"/>
    <dbReference type="EnsemblBacteria" id="BAD39538">
        <property type="protein sequence ID" value="BAD39538"/>
        <property type="gene ID" value="STH553"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH553"/>
    <dbReference type="eggNOG" id="COG3457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060710_0_0_9"/>
    <dbReference type="OMA" id="AFRTQIF"/>
    <dbReference type="OrthoDB" id="357103at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="1">
        <location>
            <begin position="33"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="BAD39538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="383" mass="40800" checksum="86A96EBCFF679004" modified="2004-10-11" version="1">MFLEVTARRNPRLIDAALRFHAEGRLPPNTYVLDLDAIRNNARTLAEAARREGIQLYFMSKQLNRNPQVIAAVAEAGIPRGVAVDIDDARAFREAGVALGHVGHLVQIPRAFLPQVLSWRPEVMTVFGLAQARAVSDAAVGLGLRQDVLLRVASGCLYPGQEGGIPLGELPAVALAISRLPGVRVAGVTAFPCLVAEDGRPVATPNLEAVTRAAALLRDELGMEIAQVNAPSLTCAATLALLRQAGATHGEPGHAITGTTPLHAVSDQPELPAMVYLTEVSHTYGGRAYVYGGGSYPRGHMQNALVGTALRRAPLHRPAAENIDYYLTLDDPLAREGEPVVAAFRTQAFTARANLALVEGVQQGAPRLAGLYDRANRPLDLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q67SS3</accession>
    <name>Q67SS3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase, partial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD39270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH285"/>
    <dbReference type="EnsemblBacteria" id="BAD39270">
        <property type="protein sequence ID" value="BAD39270"/>
        <property type="gene ID" value="STH285"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH285"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036902_12_1_9"/>
    <dbReference type="OMA" id="KFERYIG"/>
    <dbReference type="OrthoDB" id="655500at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD39270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="137" mass="15770" checksum="76B0932BE527F6F2" modified="2004-10-11" version="1">MHEVRVIHAKLELGEVGDLRRYADWRQLRKLAGYNLTENTSGQRDRSRTPISKRGRPGLRHVLYQMAMTAAAKNREFRALYEHFRTRQQNPLKGKQALVALACKLLRVLFTLVRKQCSYDPARALGAWRAEQLGLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-10-11" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q67TK3</accession>
    <name>Q67TK3_SYMTH</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">STH5</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Symbiobacterium thermophilum (strain T / IAM 14863)</name>
        <dbReference type="NCBI Taxonomy" id="292459"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Clostridia</taxon>
            <taxon>Eubacteriales</taxon>
            <taxon>Symbiobacteriaceae</taxon>
            <taxon>Symbiobacterium</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2004" name="Nucleic Acids Res." volume="32" first="4937" last="4944">
            <title>Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism.</title>
            <authorList>
                <person name="Ueda K."/>
                <person name="Yamashita A."/>
                <person name="Ishikawa J."/>
                <person name="Shimada M."/>
                <person name="Watsuji T."/>
                <person name="Morimura K."/>
                <person name="Ikeda H."/>
                <person name="Hattori M."/>
                <person name="Beppu T."/>
            </authorList>
            <dbReference type="PubMed" id="15383646"/>
            <dbReference type="DOI" id="10.1093/nar/gkh830"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">T / IAM 14863</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AP006840">
        <property type="protein sequence ID" value="BAD38990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011194140.1">
        <property type="nucleotide sequence ID" value="NC_006177.1"/>
    </dbReference>
    <dbReference type="STRING" id="292459.STH5"/>
    <dbReference type="EnsemblBacteria" id="BAD38990">
        <property type="protein sequence ID" value="BAD38990"/>
        <property type="gene ID" value="STH5"/>
    </dbReference>
    <dbReference type="KEGG" id="sth:STH5"/>
    <dbReference type="HOGENOM" id="CLU_173118_1_1_9"/>
    <dbReference type="OrthoDB" id="2054164at2"/>
    <dbReference type="Proteomes" id="UP000000417">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007169">
        <property type="entry name" value="RemA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04025">
        <property type="entry name" value="DUF370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="BAD38990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000000417"/>
        </source>
    </evidence>
    <sequence length="83" mass="8960" checksum="DD186E5EC87BDB8B" modified="2004-10-11" version="1">MYLHVGADVVVALRQVIAIVNLRTVRKGGPTQELLDKLRRENRLIQVEGGEARSLVLTDAGGILSPISATTLKRRSESAVLLG</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>